From pslath at gwu.edu Wed Aug 2 18:06:33 2023 From: pslath at gwu.edu (Patricia Latham) Date: Wed, 2 Aug 2023 19:06:33 -0400 Subject: [Histonet] tissue in alcohol too long now brittle In-Reply-To: <005801d9aaa2$bfa5d380$3ef17a80$@biopath.org> References: <005801d9aaa2$bfa5d380$3ef17a80$@biopath.org> Message-ID: Hello, Would anyone be able to provide me with a digital copy of the operator manual for a Thermoshandon Histocentre 3 embedding station? If so, please send to my email. Thank you, Patricia (pslath at gwu.edu) On Thu, Jun 29, 2023 at 12:00?PM Paula via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello, > > This is a question for a colleague of mine. Her routine tissue samples were > left in 95% alcohol for 2 hours before she had a chance to get to it. She > finished the processing, but now the gi's are extremely brittle and have > holes. She is afraid of reprocessing and doesn't know what to do. > > Can anyone who experienced this have a remedy to share? > > Thank you, > > Paula > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From acanabal at ciencias.unam.mx Fri Aug 4 13:17:27 2023 From: acanabal at ciencias.unam.mx (=?UTF-8?Q?Alonso_Mart=C3=ADnez_Canabal?=) Date: Fri, 4 Aug 2023 12:17:27 -0600 Subject: [Histonet] maximum thickness brain in slide Message-ID: Good afternoon Histoneters, I have been doing IHC and IF during decades in rodent brain tissue, always in free floating. Now, I would like to do that sections placed over slides and perform the whole procedure over the slide. I am interested in using thick sections, usually I used 50micrometers, what is the maximum thickness that I could use in slide to guarantee the correct penetration of the antibodies and the ABC complex. Before the IHC I always use graded alcohols and xilene, and antigen retrieval with citrate buffer. Thank you! -- Dr. Alonso Mart?nez Canabal PhD Profesor Asociado "C" Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833 From cforster at umn.edu Fri Aug 4 13:28:52 2023 From: cforster at umn.edu (Colleen Forster) Date: Fri, 4 Aug 2023 13:28:52 -0500 Subject: [Histonet] maximum thickness brain in slide In-Reply-To: References: Message-ID: Alonso, For sections that thick you would collect them as you always have, stain them as floating sections and then mount onto a glass slide. The routine standard methods for IHC will never penetrate a 50um thick section. Colleen Forster HT(ASCP)QIHC On Fri, Aug 4, 2023 at 1:17?PM Alonso Mart?nez Canabal via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good afternoon Histoneters, > I have been doing IHC and IF during decades in rodent brain tissue, > always in free floating. Now, I would like to do that sections placed over > slides and perform the whole procedure over the slide. I am interested in > using thick sections, usually I used 50micrometers, what is the maximum > thickness that I could use in slide to guarantee the correct penetration of > the antibodies and the ABC complex. Before the IHC I always use graded > alcohols and xilene, and antigen retrieval with citrate buffer. > Thank you! > > -- > Dr. Alonso Mart?nez Canabal PhD > Profesor Asociado "C" > Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM > Investigador Nacional "I" > 56224833 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From acanabal at ciencias.unam.mx Fri Aug 4 14:05:08 2023 From: acanabal at ciencias.unam.mx (=?UTF-8?Q?Alonso_Mart=C3=ADnez_Canabal?=) Date: Fri, 4 Aug 2023 13:05:08 -0600 Subject: [Histonet] maximum thickness brain in slide In-Reply-To: References: Message-ID: Hi, thank you for your reply. I cannot do my usual free floating staining because this tissue is very brittle. So, I was thinking to do the staining in slide at 30 microns? Thank you El vie, 4 ago 2023 a las 12:29, Colleen Forster () escribi?: > Alonso, > > For sections that thick you would collect them as you always have, stain > them as floating sections and then mount onto a glass slide. The routine > standard methods for IHC will never penetrate a 50um thick section. > > Colleen Forster HT(ASCP)QIHC > > > > On Fri, Aug 4, 2023 at 1:17?PM Alonso Mart?nez Canabal via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Good afternoon Histoneters, >> I have been doing IHC and IF during decades in rodent brain tissue, >> always in free floating. Now, I would like to do that sections placed over >> slides and perform the whole procedure over the slide. I am interested in >> using thick sections, usually I used 50micrometers, what is the maximum >> thickness that I could use in slide to guarantee the correct penetration >> of >> the antibodies and the ABC complex. Before the IHC I always use graded >> alcohols and xilene, and antigen retrieval with citrate buffer. >> Thank you! >> >> -- >> Dr. Alonso Mart?nez Canabal PhD >> Profesor Asociado "C" >> Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM >> Investigador Nacional "I" >> 56224833 >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > -- > Colleen Forster HT(ASCP)QIHC > BLS Histology and IHC Laboratory > Jackson Hall, Room 2-155 > 321 Church St. SE > Minneapolis, MN 55455 > 612-626-1930 > > > > > > > -- Dr. Alonso Mart?nez Canabal PhD Profesor Asociado "C" Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833 From cforster at umn.edu Fri Aug 4 14:55:44 2023 From: cforster at umn.edu (Colleen Forster) Date: Fri, 4 Aug 2023 14:55:44 -0500 Subject: [Histonet] maximum thickness brain in slide In-Reply-To: References: Message-ID: Even 30um is too thick for the routine IHC on glass. You will never penetrate the whole thickness for staining. Colleen Forster HT(ASCP)QIHC On Fri, Aug 4, 2023 at 2:05?PM Alonso Mart?nez Canabal < acanabal at ciencias.unam.mx> wrote: > Hi, thank you for your reply. > I cannot do my usual free floating staining because this tissue is > very brittle. So, I was thinking to do the staining in slide at 30 microns? > Thank you > > El vie, 4 ago 2023 a las 12:29, Colleen Forster () > escribi?: > >> Alonso, >> >> For sections that thick you would collect them as you always have, stain >> them as floating sections and then mount onto a glass slide. The routine >> standard methods for IHC will never penetrate a 50um thick section. >> >> Colleen Forster HT(ASCP)QIHC >> >> >> >> On Fri, Aug 4, 2023 at 1:17?PM Alonso Mart?nez Canabal via Histonet < >> histonet at lists.utsouthwestern.edu> wrote: >> >>> Good afternoon Histoneters, >>> I have been doing IHC and IF during decades in rodent brain >>> tissue, >>> always in free floating. Now, I would like to do that sections placed >>> over >>> slides and perform the whole procedure over the slide. I am interested in >>> using thick sections, usually I used 50micrometers, what is the maximum >>> thickness that I could use in slide to guarantee the correct penetration >>> of >>> the antibodies and the ABC complex. Before the IHC I always use graded >>> alcohols and xilene, and antigen retrieval with citrate buffer. >>> Thank you! >>> >>> -- >>> Dr. Alonso Mart?nez Canabal PhD >>> Profesor Asociado "C" >>> Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM >>> Investigador Nacional "I" >>> 56224833 >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> -- >> Colleen Forster HT(ASCP)QIHC >> BLS Histology and IHC Laboratory >> Jackson Hall, Room 2-155 >> 321 Church St. SE >> Minneapolis, MN 55455 >> 612-626-1930 >> >> >> >> >> >> >> > > -- > Dr. Alonso Mart?nez Canabal PhD > Profesor Asociado "C" > Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM > Investigador Nacional "I" > 56224833 > > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From paula at excaliburpathology.com Fri Aug 4 15:32:31 2023 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Fri, 4 Aug 2023 20:32:31 +0000 (UTC) Subject: [Histonet] maximum thickness brain in slide In-Reply-To: References: Message-ID: <129306712.89270.1691181151402@mail.yahoo.com> Hi, we were trained to section human brain at 8-12 ?m. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Friday, August 4, 2023 at 03:03:44 PM CDT, Colleen Forster via Histonet wrote: Even 30um is too thick for the routine IHC on glass. You will never penetrate the whole thickness for staining. Colleen Forster HT(ASCP)QIHC On Fri, Aug 4, 2023 at 2:05?PM Alonso Mart?nez Canabal < acanabal at ciencias.unam.mx> wrote: > Hi, thank you for your reply. >? ? I cannot do my usual free floating staining because this tissue is > very brittle. So, I was thinking to do the staining in slide at 30 microns? > Thank you > > El vie, 4 ago 2023 a las 12:29, Colleen Forster () > escribi?: > >> Alonso, >> >> For sections that thick you would collect them as you always have, stain >> them as floating sections and then mount onto a glass slide. The routine >> standard methods for IHC will never penetrate a? 50um thick section. >> >> Colleen Forster HT(ASCP)QIHC >> >> >> >> On Fri, Aug 4, 2023 at 1:17?PM Alonso Mart?nez Canabal via Histonet < >> histonet at lists.utsouthwestern.edu> wrote: >> >>> Good afternoon Histoneters, >>>? ? ? ? I have been doing IHC and IF during decades in rodent brain >>> tissue, >>> always in free floating. Now, I would like to do that sections placed >>> over >>> slides and perform the whole procedure over the slide. I am interested in >>> using thick sections, usually I used 50micrometers, what is the maximum >>> thickness that I could use in slide to guarantee the correct penetration >>> of >>> the antibodies and the ABC complex. Before the IHC I always use graded >>> alcohols and xilene, and antigen retrieval with citrate buffer. >>>? ? ? Thank you! >>> >>> -- >>> Dr. Alonso Mart?nez Canabal PhD >>> Profesor Asociado "C" >>> Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM >>> Investigador Nacional "I" >>> 56224833 >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> -- >> Colleen Forster HT(ASCP)QIHC >> BLS Histology and IHC Laboratory >> Jackson Hall, Room 2-155 >> 321 Church St. SE >> Minneapolis, MN 55455 >> 612-626-1930 >> >> >> >> >> >> >> > > -- > Dr. Alonso Mart?nez Canabal PhD > Profesor Asociado "C" > Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM > Investigador Nacional "I" > 56224833 > > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Sat Aug 5 12:46:19 2023 From: carl.hobbs at kcl.ac.uk (Carl Hobbs) Date: Sat, 5 Aug 2023 17:46:19 +0000 Subject: [Histonet] maximum thickness brain in slide Message-ID: Hi Alonso Firstly, what antibody( ies) are you wanting to use on your Pwax sections? If you are looking just to identify proteins, 8 microns is sufficient I mount my brain sections ( human, ms, rat, axolotl, worm, fruitfly, pig...etc) then airdry them O/N in a working fume hood Then, before dewaxing, I put them into a 60C oven for 1hr. Imho, free-floating sections are useful only if you wish to study projections over depth Use Superfrost Plus ( or equivilent) slides Good luck! Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 From relia1 at earthlink.net Wed Aug 9 13:13:39 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 9 Aug 2023 14:13:39 -0400 Subject: [Histonet] Happy Hump Day Histopeeps!! Summer is Almost OVER!!! Here are some exciting new opportunities for you and your friends! Message-ID: <005201d9caed$3978fab0$ac6af010$@earthlink.net> Happy Hump Day Histopeeps!! It is hard to believe the summer is almost over. The kids are going back to school and Labor Day is just a few weeks away! A few more weeks after that ?Halloween; Then Thanksgiving, Christmas, and New Year?s Day!! ? Looking for a new job in the Fall? ? Planning a job change after the holidays? ? Considering making a move in 2024? Let?s Get The Ball Rolling!!! Histonetters! **Are you a histo tech looking for something better? **Are you a new or recent graduate of a histology school? **Are you a traveler transitioning into a permanent position? **Are you a Histotech looking to advance into leadership? **Are you a Supervisor looking to advance to lab management? Let?s Get The Ball Rolling!!! All you have to do is contact me at relia1 at earthlink.net or call/text me at 407-353-5070 or call me at 866-607-3542 my office is open M-F 7am-8pm EST or by appointment. If you know of anyone else who might be interested in subscribing to RELIA?s Histology Careers Bulletin, please feel free to pass this along to them. I have exciting opportunities in: ? Leadership ? Tech Support ? Mohs ? GI ? Derm ? Clinical ? AP ? Research These Opportunities are located in: ? Florida ? Brand New Lab! ? Washington ? South Carolina ? Arizona ? California ? Massachusetts ? Wisconsin ? Tennessee ? Georgia ? Alabama I invite you to join my group on Facebook: www.facebook.com/groups/histotechnologists Have a Great Week! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions I invite you to join my group on Facebook: www.facebook.com/groups/histotechnologists Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From BMolinari at texasheart.org Thu Aug 10 09:57:31 2023 From: BMolinari at texasheart.org (Betsy Molinari) Date: Thu, 10 Aug 2023 14:57:31 +0000 Subject: [Histonet] Sudan Black B References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.4370ed1c-b687-417b-a251-fd39c31d9bdc@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.b622bf03-c301-47fa-a9ff-dc103afc07bf@emailsignatures365.codetwo.com> Message-ID: Hi, I have been asked to do a Sudan stain on a heart biopsy for lipofuscin. The biopsy is in a paraffin block. They are looking to better report and understand the IHC. I am totally unfamiliar with this stain. I did some reading but have been unable to find a protocol for paraffin sections. I found a reference to Sheehan & Hrapchak (1973) but unfortunately I don't have that edition. Any ideas would be greatly appreciated . Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 1101 Bates St. Houston, Texas 77030 832-355-6524 Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research The Texas Heart Institute (r) 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | texasheartmedical.org | facebook | twitter CONFIDENTIALITY NOTICE: This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From jkiernan at uwo.ca Thu Aug 10 19:11:02 2023 From: jkiernan at uwo.ca (John Kiernan) Date: Fri, 11 Aug 2023 00:11:02 +0000 Subject: [Histonet] Sudan Black B In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.4370ed1c-b687-417b-a251-fd39c31d9bdc@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.b622bf03-c301-47fa-a9ff-dc103afc07bf@emailsignatures365.codetwo.com> Message-ID: The Sudan black B method for lipofuscin is exactly the same as for frozen sections, but it is applied to hydrated paraffin sections. Remember that the dye solution needs to be fairly fresh (less than 4 weeks old) and must be filtered immediately before using. Use Sudan black B (CI 26150) powder that has been certified by the Biological Stain Commission. Various controls and other stains are needed to be certain that the pigment is lipofuscin, not melanin or haemosiderin. John Kiernan Anatomy, UWO, London, Canada = = = ________________________________ From: Betsy Molinari via Histonet Sent: August 10, 2023 10:57 AM To: Histonet Subject: [Histonet] Sudan Black B Hi, I have been asked to do a Sudan stain on a heart biopsy for lipofuscin. The biopsy is in a paraffin block. They are looking to better report and understand the IHC. I am totally unfamiliar with this stain. I did some reading but have been unable to find a protocol for paraffin sections. I found a reference to Sheehan & Hrapchak (1973) but unfortunately I don't have that edition. Any ideas would be greatly appreciated . Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 1101 Bates St. Houston, Texas 77030 832-355-6524 Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research The Texas Heart Institute (r) 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | texasheartmedical.org | facebook | twitter CONFIDENTIALITY NOTICE: This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From: Betsy Molinari via Histonet Sent: August 10, 2023 10:57 AM To: Histonet Subject: [Histonet] Sudan Black B Hi, I have been asked to do a Sudan stain on a heart biopsy for lipofuscin. The biopsy is in a paraffin block. They are looking to better report and understand the IHC. I am totally unfamiliar with this stain. I did some reading but have been unable to find a protocol for paraffin sections. I found a reference to Sheehan & Hrapchak (1973) but unfortunately I don't have that edition. Any ideas would be greatly appreciated . Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 1101 Bates St. Houston, Texas 77030 832-355-6524 Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research The Texas Heart Institute (r) 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | texasheartmedical.org | facebook | twitter CONFIDENTIALITY NOTICE: This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Tue Aug 15 12:37:29 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 15 Aug 2023 13:37:29 -0400 Subject: [Histonet] RELIA HOT JOB ALERT! Exciting NEW Opportunities in FL, WA, TN, SC, AL, AZ, GA, WI, CA and more Message-ID: <01e301d9cf9f$2b0ea4f0$812beed0$@earthlink.net> Hi Histopeeps! I hope you are having a great day! I have exciting NEW Opportunities in FL,WA,TN,SC,AL,AZ,GA,WI,CA and more Leadership and Tech opportunities! Learn and GROW Some of these are RELIA Exclusives! Some are BRAND NEW LABS!! All of these clients are top tier employers offering excellent compensation, benefits and relo/sign on bonuses. My clients will wait until your contract ends. You can take the time off you have already scheduled. For more info you can contact me. Facebook/Instagram/LinkedIN/Twitter: message me!! call/text 407-353-5070 email me: relia1 at earthlink.net I welcome you to join my Facebook group: www.facebook.com/groups/histotechnologists Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at earthlink.net Thu Aug 17 11:21:11 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 17 Aug 2023 12:21:11 -0400 Subject: [Histonet] COOL AI Art Created Using Histology and HOT Histology Career Opportunities! Message-ID: <017e01d9d126$d6ec19a0$84c44ce0$@earthlink.net> Hi Histopeeps! I hope you are having a great day. I have something to share that I think is pretty cool and also some pretty HOT job opportunities! See what I did there? Cool Stuff First: I have been playing around with AI art and have generated a few images that are pretty cool. Due to the limits on Histonet I can't post them. Here is a list: 1. American Flag created using histology stains 2. A rainbow created using histology stains 3. Fireworks created using histology stains 4. Aaand my personal favorite - "A Histopeep" If you would like a copy of any of these images just shoot me an email to relia1 at earthlink.net and I will send them to you as jpg files. I also have some HOT histology job opportunities: South Carolina, Tennessee Florida California Arizona Washington Georgia Wisconsin Alabama Georgia All of my clients offer excellent compensation, benefits and some offer relocation assistance and or sign on bonuses. All of these jobs are full time & permanent Some are RELIA EXCLUSIVES and Some are Brand NEW LABS! NEW POSITIONS ARE COMING IN DAILY NATIONWIDE!!! So, Drop me a line and let me know where you want to go!! If you or anyone you know is interested in hearing more about any of these opportunities, please contact me. Remember! if I place someone you refer to me you will earn a referral bonus!!! I can be reached anytime on my cell/text at 407-353-5070 or toll free at 866-607-3542 or e-mail me at relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From beth.oneil1 at wvumedicine.org Wed Aug 23 07:27:11 2023 From: beth.oneil1 at wvumedicine.org (O'Neil, Beth) Date: Wed, 23 Aug 2023 12:27:11 +0000 Subject: [Histonet] Leica H&E 600 Message-ID: I would appreciate any feedback from anyone with experience using the Leica H&E 600. I recently attended a demo of the unit and we are still hung up on the large slide tray it uses instead of the traditional slide racks. I also spoke with another user who indicated that if you need to re-coverslip a slide it is very difficult to remove the existing coverslip and when you do the hematoxylin is removed from the stain ??? We already know we will need two units to handle our workload of approximately 900 H&E. Any information would be greatly appreciated. Beth O'Neil beth.oneil1 at wvumedicine.org West Virginia University, JW Ruby Memorial Hospital Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Donna.Willis at BSWHealth.org Wed Aug 23 07:53:06 2023 From: Donna.Willis at BSWHealth.org (Willis, Donna G) Date: Wed, 23 Aug 2023 12:53:06 +0000 Subject: [Histonet] Leica H&E 600 In-Reply-To: References: Message-ID: Good Morning Beth, The HE600 is a Roche product. The slides are stained flat with reagents being applied to each slide individually instead of batch staining like other instruments. This allows you to get the same staining quality on EACH slide. We have used the instrument in our lab since 2005 and got a second unit when we moved into a larger location 2 years ago. Coverslips are removed by apply heat from a hot plate and we have no issues with the hematoxylin if we remove a coverslip. We rarely have issues with coverslips that we need to remove one. Let me know if you have any other questions. Donna Willis HT/HTL (ASCP) Anatomic Pathology Manager Baylor Scott&White Health Baylor University Medical Center 3500 Gaston Ave. Dallas, Texas 75246 214-820-2465 office 214-725-6184 cell -----Original Message----- From: O'Neil, Beth via Histonet Sent: Wednesday, August 23, 2023 7:27 AM To: Histonet Subject: {EXTERNAL} [Histonet] Leica H&E 600 CAUTION:??This email originated outside of BSWH. The actual sender is (histonet-bounces at lists.utsouthwestern.edu) which may be different from the display address in the From: field. Avoid action unless you know the content is safe. Report suspicious emails using the PhishAlarm button located in your Outlook ribbon. I would appreciate any feedback from anyone with experience using the Leica H&E 600. I recently attended a demo of the unit and we are still hung up on the large slide tray it uses instead of the traditional slide racks. I also spoke with another user who indicated that if you need to re-coverslip a slide it is very difficult to remove the existing coverslip and when you do the hematoxylin is removed from the stain ??? We already know we will need two units to handle our workload of approximately 900 H&E. Any information would be greatly appreciated. Beth O'Neil beth.oneil1 at wvumedicine.org West Virginia University, JW Ruby Memorial Hospital Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!B6DvbYNaHNlr30S82_hQPy1iaJmOC83cw1cJ_Zo7eBt3Kjh06zRSuqlJBZqK3p7EmcpSulBVTLEaqBnj6kL3GMDm_ksorGDbgU4_Dg$ ********************************************************************** The information contained in this e-mail may be privileged, confidential, and/or protected from disclosure. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly prohibited and no waiver of any attorney-client, work product, or other privilege is intended. No binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, is permitted by e-mail without express written confirmation by a duly authorized representative of Baylor Scott & White Health. From bcooper at chla.usc.edu Wed Aug 23 08:52:44 2023 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 23 Aug 2023 13:52:44 +0000 Subject: [Histonet] Leica H&E 600 In-Reply-To: References: Message-ID: The Ventana HE600 is an awesome machine. You can't use xylene to remove the coverslips, you need to heat them on a hot plate to soften the glue, and then the slips just come right off. I used other dip and dunk stainers in the past and I was skeptical at first too. Those concerns have been put to rest! The machine requires no manual user maintenance (PM service is of course required) or filtering of reagents. You can't put expired reagents on the machine, nor can you put reagents in the wrong place. The machine performs a cleaning cycle every evening, so we literally do nothing on this machine except press start. No machine is completely perfect. Sometimes staining modules leak or fail. This machine has 3 separate staining modules so you can always disable the troubled module and the machine will still work. It's been very rare that our coverslipper has failed. It's certainly not the cheapest stainer on the market, but I've told people who come to our lab that the machine is like another employee! It just works and works and works. I'll be happy to answer any further questions. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu ________________________________ From: O'Neil, Beth via Histonet Sent: Wednesday, August 23, 2023 5:28:11 AM To: Histonet Subject: [Histonet] Leica H&E 600 (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. I would appreciate any feedback from anyone with experience using the Leica H&E 600. I recently attended a demo of the unit and we are still hung up on the large slide tray it uses instead of the traditional slide racks. I also spoke with another user who indicated that if you need to re-coverslip a slide it is very difficult to remove the existing coverslip and when you do the hematoxylin is removed from the stain ??? We already know we will need two units to handle our workload of approximately 900 H&E. Any information would be greatly appreciated. Beth O'Neil beth.oneil1 at wvumedicine.org West Virginia University, JW Ruby Memorial Hospital Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1TXiKtX-ZXeWSB5YN4ws_LAt1PaJWjPbNmfwbFI3RJeA5utxKLfq1Uyp4p9Eq9jpOzmY8X29yLiB6bViNANRItOGrPMv2h_fHASzepn1yMhtCPPVi2iw89GTSGQgOzTSwlSvdMNqxjXTJ6w1X3LsT3OIjlWEkIrf8moU04NZ3QRWHlXY4WaPQVnQq3gG47Fyv8MQ1QLPRzt4hrph_zD9VPXbW4OqiZikCWfhwqSGrXEdwAwrGgfZPGB5UoWZ2yHszYxZvXR9-aflXyVqlSO_j4DcHMo2ye8LgzgSYzCv9qc4G_vszrrgPFwL6Secvrt7lbDaJLARWiuedwxAU0zmbo5fQMl0o-zhDkq3xWz7wOKiX-3AVjOSYn1QP7zWAGDWhuMACrKrTjK_YYkeW3wJCw_e9CZ3PBkT6CwlM869wl1iW3CUEQosvULNlTmiiykIeQZfpo22o1K1IYkbzLS6RRA/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From Nancy.Schmitt at mercyhealth.com Thu Aug 24 14:21:05 2023 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Thu, 24 Aug 2023 19:21:05 +0000 Subject: [Histonet] moh's staining protocol Message-ID: Hello- What is a best staining protocol for Moh's? Is there one that starts in alcohol and ends in alcohol? With no xylene or formalin? Your thoughts are appreciated, Nancy Schmitt, MLT(ASCP) CM HT CM Pathology Support Services Supervisor Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From rmccormick10 at yahoo.com Wed Aug 30 13:42:28 2023 From: rmccormick10 at yahoo.com (Rhonda McCormick) Date: Wed, 30 Aug 2023 18:42:28 +0000 (UTC) Subject: [Histonet] Gram Stain References: <101525110.2730271.1693420948027.ref@mail.yahoo.com> Message-ID: <101525110.2730271.1693420948027@mail.yahoo.com> Howdy,Does anyone have a recommendation for a Gram stain (or modification of a gram stain) that?stains the background yellow with red gram-negative bacteria? (no blue - gram positive staining).Thank you! From jkiernan at uwo.ca Thu Aug 31 00:45:04 2023 From: jkiernan at uwo.ca (John Kiernan) Date: Thu, 31 Aug 2023 05:45:04 +0000 Subject: [Histonet] Gram Stain In-Reply-To: <101525110.2730271.1693420948027@mail.yahoo.com> References: <101525110.2730271.1693420948027.ref@mail.yahoo.com> <101525110.2730271.1693420948027@mail.yahoo.com> Message-ID: I don't know a method to obtain selective red coloration of Gram-negative organisms, with yellow for both Gram-positives and "background", and I cannot find one by looking in various books. It may not be possible because Gram-positivity relies on selective retention of an immobilized dye. Gram-negative bacteria stain only because the red dye is excluded by the organisms already filled with an insolubilized blue-purple dye in the Gram-positive cells. The Brown-Hopps modification of Gram staining for paraffin sections clearly separates blue from red bacteria and gives a quite different yellowy-brown counterstain to the "tissue background". This is explained and well illustrated in Freida Carson's textbook. (I have only the 2nd edition, 1997; there is now a 5th, 2020, ISBN 9780891896760.) John Kiernan (London, Ontario) = = = ________________________________ From: Rhonda McCormick via Histonet Sent: August 30, 2023 2:42 PM To: Histonet Subject: [Histonet] Gram Stain Howdy,Does anyone have a recommendation for a Gram stain (or modification of a gram stain) that stains the background yellow with red gram-negative bacteria (no blue - gram positive staining).Thank you! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet