From kunkeesh at gmail.com Thu Sep 1 12:52:57 2022 From: kunkeesh at gmail.com (kidist Shamebo) Date: Thu, 1 Sep 2022 12:52:57 -0500 Subject: [Histonet] Histonet Digest, Vol 226, Issue 1 In-Reply-To: References: Message-ID: On Thu, Sep 1, 2022 at 12:00 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Automated ihc staining of bone (Bacon, Charles) > > > > ---------- Forwarded message ---------- > From: "Bacon, Charles" > To: "histonet at lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Wed, 31 Aug 2022 17:23:34 +0000 > Subject: [Histonet] Automated ihc staining of bone > When we have section lifting during IHC, we will use a concentrated Poly-L > Lysine solution. You can dilute this solution but instead we will use a > direct application (wet the tip of a gloved finger with the solution, and > wipe down the slide, let dry) on one of our charged slides. This may cause > a degree of counter stain background but always improves the IHC result for > the pathologist. We use this for bone or heavily keratinized skin sections. > > Supplies: > Poly-L: Sigma Diagnostics? Poly-L-lysine Solution - SDP8920A > Slides: TYPENEX Medical Microscope Slides - PS0101P > > Chuck Bacon, HTL(ASCP)CM > Supervisor Histology > Baystate Medical Center > 361 Whitney Ave., Holyoke, MA 01040 > Telephone: 413-322-4786 Fax: 413-322-4790 > Charles.Bacon at baystatehealth.org > > -----Original Message----- > From: histonet-request at lists.utsouthwestern.edu < > histonet-request at lists.utsouthwestern.edu> > Sent: Wednesday, August 31, 2022 1:00 PM > To: histonet at lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 225, Issue 14 > > ? EXTERNAL EMAIL > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!P_vj-BUkwjF5!d9CyRRxx8zNIrA1NS-jWvW3aHfJ5ev1n6ROMxAqGaYdNfpEYN2i1jrtktmUJiY9znlYtKzBsQAgBvOfvV-hhR885RRn6ajjdI8N0LLdY3a56XfA$ > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific than > "Re: Contents of Histonet digest..." > > EXTERNAL EMAIL: This email came from outside of Baystate. Please use > extreme caution before clicking on any links or opening any attachments. > > ---------------------------------------------------------------------- > Please view our annual report at http://www.bhannualreport.org > > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may > contain confidential and privileged information for the use of the > designated recipients named above. If you are not the intended recipient, > you are hereby notified that you have received this communication in error > and that any review, disclosure, dissemination, distribution or copying of > it or its contents is prohibited. If you have received this communication > in error, please reply to the sender immediately or by telephone at > 413-794-0000 and destroy all copies of this communication and any > attachments. For further information regarding Baystate Health's privacy > policy, please visit our Internet site at https://www.baystatehealth.org. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy.Schmitt at mercyhealth.com Fri Sep 2 09:56:01 2022 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Fri, 2 Sep 2022 14:56:01 +0000 Subject: [Histonet] Cytology and Base mold release Message-ID: Happy Friday (unless you work the weekend), 1. Cytology - when cotest is ordered for Pap/HPV do you hold reading the pap until the HPV is completed? Is there a recommendation on this? 2. Histology - when using base mold release, do you spray the molds once at the start of embedding, or in between every block embedded? Your thoughts are appreciated, Nancy Schmitt Pathology Support Services Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From greg.dobbin at gmail.com Fri Sep 2 13:05:38 2022 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Fri, 2 Sep 2022 15:05:38 -0300 Subject: [Histonet] Processing artefact?? Message-ID: Hello experts, Every now and then we will get stained slides that look like the attached image. We run one processing schedule (about 11 hrs) for everything so yes, our smaller biopsies are somewhat overprocessed, but 90-95% of the time they look perfectly fine. We can have GI biopsies in a case that go from A to P and find only one that looks like this. Moreover, recuts do not remedy the problem either...the problem seems to be occurring prior to microtomy. We use vendor grade Xylenes in our processor (a Leica ASP6025). All specimens are fixed for 18-24 hours prior to processing. Our GI blocks get a short soak in phenol prior to microtomy to help with the effects of overprocessing. All blocks within a case are treated the same. In fact we have also seen it in skin biopsies occasionally and skins do not get soaked in phenol. It is the randomness of the problem that is throwing us! Any ideas? Thanks, *Greg Dobbin* Chief Technologist Anatomic Pathology Queen Elizabeth Hospital Charlottetown, PE, Canada From greg.dobbin at gmail.com Tue Sep 6 08:24:34 2022 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Tue, 6 Sep 2022 10:24:34 -0300 Subject: [Histonet] Processing artefact?? Message-ID: Since images cannot be attached, if any of you feel you have some experience that I may find helpful...please email me directly and I will attach the image to my reply. Thanks again, *Greg Dobbin* Chief Technologist Anatomic Pathology Queen Elizabeth Hospital Charlottetown, PE, Canada From edmartin26 at gmail.com Tue Sep 6 12:01:51 2022 From: edmartin26 at gmail.com (Eddie Martin) Date: Tue, 6 Sep 2022 13:01:51 -0400 Subject: [Histonet] Histonet Digest, Vol 225, Issue 14 In-Reply-To: References: Message-ID: Changing your IHC slides to another brand with stronger slide adhesion is suggested when working with bone and very fatty tissue to remain on the slide. shaking off as much moisture from the slide rack before placing them to dry in the oven is another. Some animal tissues benefit from leaving in the oven overnight at 60 degrees celsius. regarding slides with better tissue section adhesion, our bone marrow pathology laboratory likes using the Azer Scientific's EMS200W+ charged slides. There's another brand that's great for human brain, breast and also animal tissues: I believe they're called Autofrost by Cancer diagnostics (but I'm not sure if it's these, as the slides were used mostly at another laboratory I used to work at five years ago). I hope this all helps! Best regards, Eddie Martin, HTL, QIHC Bethesda, MD On Wed, Aug 31, 2022 at 1:00 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Automated ihc staining of bone (Charles Riley) > 2. Re: Automated ihc staining of bone (Cooper, Brian) > 3. Information on the resistance/viability of different > tissues/cells to different freezing temperatures? (Alida Bailleul) > > > > ---------- Forwarded message ---------- > From: Charles Riley > To: histonet at lists.utsouthwestern.edu > Cc: > Bcc: > Date: Tue, 30 Aug 2022 15:50:18 -0400 > Subject: [Histonet] Automated ihc staining of bone > I am working on trying to get IHC stains optimized on bone samples of rat > tibias. The issue I am running into is that the periosteum is > separating from the bone marrow and wrinkling up over top of the rest of > the sample. > > If anyone has any techniques to prevent this wrinkling/detachment of tissue > from the main section during automated ihc staining it would be greatly > appreciated. > > > > > ---------- Forwarded message ---------- > From: "Cooper, Brian" > To: Charles Riley , "histonet at lists.utsouthwestern.edu" < > histonet at lists.utsouthwestern.edu> > Cc: > Bcc: > Date: Tue, 30 Aug 2022 20:09:14 +0000 > Subject: Re: [Histonet] Automated ihc staining of bone > Charles, > > Try and blot your sections dry before placing them into the oven. After > picking up your section, place your slide onto a flat surface and take a > paper towel or piece of filter paper, and gently press it down on top of > the section to wick away all of the moisture. We use this technique > frequently here on bone marrow cores. > > Thanks, > > Brian D. Cooper, HT (ASCP)CM QIHC| Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > bcooper at chla.usc.edu > > -----Original Message----- > From: Charles Riley via Histonet > Sent: Tuesday, August 30, 2022 12:50 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Automated ihc staining of bone (EXTERNAL EMAIL) > > ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from > outside CHLA. Do not open attachments, click on links, or respond unless > you expected this message and recognize the email address: > histonet-bounces at lists.utsouthwestern.edu. > > I am working on trying to get IHC stains optimized on bone samples of rat > tibias. The issue I am running into is that the periosteum is separating > from the bone marrow and wrinkling up over top of the rest of the sample. > > If anyone has any techniques to prevent this wrinkling/detachment of > tissue from the main section during automated ihc staining it would be > greatly appreciated. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > http://secure-web.cisco.com/1srYnJjJBwOg5qJEJ_epFgbAXCjZWoirSFghf1tBQDTt4Kt86glQhWXDUFKHHe2BviKDOGHc_YOZrH3k3sKiGazjO1fRhU2GFJi18RGa2bb0f-VI2JMFwgoRCh3Mv4BFJnHqPjO4A8wLuoBUVteRZSMMDezrs0-eKxExtxe_Bn1OanJnxSFmzWIQXJl9XzUCwT1uCqSj9CFXMwW4swVLbFy3qpaq_74-LnLDO6yiQlBPyYP1upb0iwltU9iWQo-lfrXjFfKlYAHruMGUbJftFh1zBiA4sOKi90ubPDFxSvFr3-Aerb35g3yHJKIG5lZ2K0RuznvEbH-EjWmb7n3uhym4J61BoaaMBF1sH4MG7XG7yiiK5CIqm9SvxKGHyM5MeQXiTb_Ehnc74qW2NkSkEur2f06DKlv742nj6i9Ge70vNqu9tXdlIft0Q4nj4mBsO/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, > please contact the sender by reply e-mail and destroy all copies of this > original message. > > > > > > > ---------- Forwarded message ---------- > From: Alida Bailleul > To: histonet > Cc: > Bcc: > Date: Wed, 31 Aug 2022 10:27:56 +0800 > Subject: [Histonet] Information on the resistance/viability of different > tissues/cells to different freezing temperatures? > Where can I find information on the resistance of different tissues/cells > (e.g., bone, cartilage) to different freezing temperatures (e.g., -4C; > -24C; -70C ) ? I am asking about frozen tissues that were not > cryopreserved. I think there may be a lot of experimental studies that > compared the resistance of cells/tissues to freeze-thaw cycles at different > temperatures (and also compared fresh frozen versus cryopreserved) but I > didn't really find a lot of studies. Moreover studies don't really compare > different tissue types, they focus on one type of tissue per study. > Please guide me towards some papers (perhaps in the old literature? Even > before cryopreservation was discovered?). > I think there might be information in papers about how to store > osteochondral grafts, but I did not find the information I was looking for. > Thank you in advance > Best wishes > > Alida > > -- > Dr. Alida M. Bailleul > Associate Professor > Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy > of Sciences > www.ivpp-avianevolution.com > & Research Associate of Paleontology, Museum of the Rockies, Montana State > University > Google Scholar > - > ResearchGate > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nmargaryan88 at gmail.com Wed Sep 7 08:30:48 2022 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Wed, 7 Sep 2022 08:30:48 -0500 Subject: [Histonet] Molds- cold vs warm In-Reply-To: <29E51433-19F4-420B-B5E4-B098EF4BE6A2@gmail.com> References: <40CBA05C-4304-40DA-81A7-BA5901024E04@sbcglobal.net> <29E51433-19F4-420B-B5E4-B098EF4BE6A2@gmail.com> Message-ID: Hello histo geeks, Could you please help me with project and provide me best reasons why using warm molds are better than cold (room temperature) as well as the opposite why not to use room temperature molds during embedding tissues. Reason I am asking that we were suggested to use cold to easily remove paraffin blocks from molds and not to spend time on melting blocks. Thanks in advance, Naira From criley at udel.edu Wed Sep 7 11:33:19 2022 From: criley at udel.edu (Charles Riley) Date: Wed, 7 Sep 2022 12:33:19 -0400 Subject: [Histonet] Discussion help Message-ID: Hello everyone, I have been asked to give a presentation on the best practices for preparing histological study samples for research on animal tendons. Can anyone provide me with resources to reference and or any practices that you use for specific animal types or are they pretty much similar across all animals? From nmargaryan88 at gmail.com Wed Sep 7 13:35:25 2022 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Wed, 7 Sep 2022 13:35:25 -0500 Subject: [Histonet] Warm vs RT molds In-Reply-To: References: Message-ID: Thank you all who answered. I also would like to get any research behind that - maybe a publication to prove. If anyone come across with scientific publications please share it with me. Thanks in advance, Naira On Wed, Sep 7, 2022 at 1:23 PM Terri Braud wrote: > Just my 2 cents. Warm molds allow better positioning closer to the bottom > of the mold, so the tissue is flat against the bottom. Chill the embedded > tissue to release the block, then place the mold on its side and any > residual paraffin and tissue contaminant will melt off and the mold will be > ready to use again. > > > > > > > *Terri L. Braud, HT(ASCP)* > > *HNL Laboratories for * > > *Holy Redeemer Hospital* > > *1648 Huntingdon Pike > * > > *Meadowbrook, PA 19046 > * > > *Ph > : > 215-938-3689* > > *Fax: 215-938-3874* > > *H**onesty* > > *Accou**N**tability* > > * Agi**L**ity* > > * Co**L**laboration* > > * Co**M**passion* > > > From jaylundgren at gmail.com Fri Sep 9 16:36:44 2022 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 9 Sep 2022 16:36:44 -0500 Subject: [Histonet] Molds- cold vs warm In-Reply-To: References: <40CBA05C-4304-40DA-81A7-BA5901024E04@sbcglobal.net> <29E51433-19F4-420B-B5E4-B098EF4BE6A2@gmail.com> Message-ID: Whoever is telling you to use cold molds needs to go back to clown college. That is totally, 100%, absolutely, wrong. There is some debate as to embed "wet" (cassettes submerged in paraffin bath) or "dry", and I will accept either, as mostly a matter of personal preference. BUT, in both of these cases, the molds are hot. I have been a Histotech for five decades, trained at Armed Forces Institute of Pathology (back when that used to mean something) and I have NEVER seen anyone using cold molds. It is a guaranteed way to get cold fractures and cracks in your blocks, or to pop tissue out when you are cutting, which might be irretrievable. Just think how much time all those re-embedded blocks are going to save you! Also, you won't be able to easily re-position specimens in the block, to put them "on edge" or "on end", for example. The tissue will instantly stick to the cold mold. And if you want to re-position it, guess what, you'll have to warm the mold up to get the tissue unstuck. How's that (non-existent anyway) time savings now? If you want to prove to whatever jackass suggested this that they are wrong, get a big stack of every histopathology textbook you can find. There is nothing in any of them talking about paraffin embedding with cold molds. As a matter of fact, every single textbook will specify molds at the same temp as paraffin. Anyway, it doesn't even make sense, thermodynamically. Heat travels from hot to cold. Those "cold" molds will be the same temperature as the paraffin, almost instantly. Did it take a tiny amount of heat out of the hot paraffin? Yes, but not enough to noticeably cool the blocks faster. The amount of heat from the paraffin used to warm the mold is trivial compared to the total heat of the system. That's why cold plates have huge, noisy refrigeration units. You can't argue with thermodynamics. If you are having trouble getting your blocks to release, use mold release! Viola! https://www.statlab.com. I used to think it was superfluous, but now I consider it compulsory. This is probably the answer to most of your issues. I don't know who is suggesting using cold molds, but I can pretty much guarantee that it's a pathologist who thinks his slides are taking too long, and knows nothing about histopathology, or a lab manager, who knows nothing about histopathology. This next part is directly to them. To Whoever Suggested Cold Molds: The answer to getting your slides out quicker is buying more equipment and hiring more techs, and holding everyone to standards (30 blocks/hr cutting, 60 blocks/hr embedding). Making nonsensical, uninformed suggestions only exposes your ignorance. Please feel free to show them this reply. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) From bcooper at chla.usc.edu Fri Sep 9 17:00:16 2022 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Fri, 9 Sep 2022 22:00:16 +0000 Subject: [Histonet] Molds- cold vs warm In-Reply-To: References: <40CBA05C-4304-40DA-81A7-BA5901024E04@sbcglobal.net> <29E51433-19F4-420B-B5E4-B098EF4BE6A2@gmail.com> , Message-ID: Thanks for saying this Jay!! I have to say, it's been a while since we've had such a great response on Histonet!! Everything you said is spot on. Happy Friday everyone! Thanks, Brian Cooper Histology Supervisor Children's Hospital Los Angeles Sent from my mobile On Sep 9, 2022 2:37 PM, Jay Lundgren via Histonet wrote: ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. Whoever is telling you to use cold molds needs to go back to clown college. That is totally, 100%, absolutely, wrong. There is some debate as to embed "wet" (cassettes submerged in paraffin bath) or "dry", and I will accept either, as mostly a matter of personal preference. BUT, in both of these cases, the molds are hot. I have been a Histotech for five decades, trained at Armed Forces Institute of Pathology (back when that used to mean something) and I have NEVER seen anyone using cold molds. It is a guaranteed way to get cold fractures and cracks in your blocks, or to pop tissue out when you are cutting, which might be irretrievable. Just think how much time all those re-embedded blocks are going to save you! Also, you won't be able to easily re-position specimens in the block, to put them "on edge" or "on end", for example. The tissue will instantly stick to the cold mold. And if you want to re-position it, guess what, you'll have to warm the mold up to get the tissue unstuck. How's that (non-existent anyway) time savings now? If you want to prove to whatever jackass suggested this that they are wrong, get a big stack of every histopathology textbook you can find. There is nothing in any of them talking about paraffin embedding with cold molds. As a matter of fact, every single textbook will specify molds at the same temp as paraffin. Anyway, it doesn't even make sense, thermodynamically. Heat travels from hot to cold. Those "cold" molds will be the same temperature as the paraffin, almost instantly. Did it take a tiny amount of heat out of the hot paraffin? Yes, but not enough to noticeably cool the blocks faster. The amount of heat from the paraffin used to warm the mold is trivial compared to the total heat of the system. That's why cold plates have huge, noisy refrigeration units. You can't argue with thermodynamics. If you are having trouble getting your blocks to release, use mold release! Viola! https://secure-web.cisco.com/1vhENcmRngDgLubdLEYMzzWWUK4ILg_WIJNnMutz67Oikk5LSg5SqF6OvSQqMWpr4MIirbF_ExGbIXm9Usdm35LUk87pXYTIvPVdKY5u2dRCdo_Ss-iuZ4nCOa0nPTIpPec8zwvOBcVIE7eM7o-flt9BAIGK0ZOw4K3HOXwNiLmQBnD0hFb9pgrU0ZuPnk5llOYCeJ5b2Pmkp2B9UPlVvxPMI3-iHRILtOB4kPL45PII_yUJnJhFYAryeid5lrITtm-w0KNyKrfJVI0mHy47Niz0TEpxxvl3DoTDmq-umsyN3BucCj2B-aJFqJ-AW3thtXSEk-Nl0NzBBrSxw8cPzSrKsVww7cCLh_krbh7VXKlRiRGF41o3UKk_oEQuHGIEeYlUNLnpLndnkSH0cwR3nNWhq3Cy8hw6ws0Ka8kYRH8_TVttsOh_lQbO4tm6_i-fdNOZxcR_7t-QeE9aW5YP1hg/https%3A%2F%2Fwww.statlab.com I used to think it was superfluous, but now I consider it compulsory. This is probably the answer to most of your issues. I don't know who is suggesting using cold molds, but I can pretty much guarantee that it's a pathologist who thinks his slides are taking too long, and knows nothing about histopathology, or a lab manager, who knows nothing about histopathology. This next part is directly to them. To Whoever Suggested Cold Molds: The answer to getting your slides out quicker is buying more equipment and hiring more techs, and holding everyone to standards (30 blocks/hr cutting, 60 blocks/hr embedding). Making nonsensical, uninformed suggestions only exposes your ignorance. Please feel free to show them this reply. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1sst_F8q-xPNVIECxucZyjCFTGbDl-OZqoDxUAqSsYz8xAR-kLrSYbAJ1I5oMBLXtOcoOm7CKoMBxRIyL2NOyKolfXTBFuF7FYiWgJio8Ku7hKdyCkzHYmQCcne4XS1yWXzU-X4Szp0h5-1FvqjOhMNcN0drsSahjrT25jvMdlloIXxuy0EcG8_c1lTs0zgq-wevTMHkxwJkdLA8z_JJDz3Kloe4S_3DcwNmZjPIfdHx3BZdIiaKRE3Gauws2kOOKMMztVdzAOkyE-MMMfyehmZCrAryOUn-qd-XRsYHczeIAYf57VjR5N7HwW_sydi3gMX7NUNiR0j5ZI8-xq4pfDE5mhnYScwa1RGqaZ3VVqG5jFDIa0Js84cz0UXMeB7PbQRxosMdOPmtRX9ycvNM1S7iijUEFk5smWvT33f1CHH_9qPWBb9KM0q-faJj10SacllYXV1K3egxhFh1DNkihHw/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From nmargaryan88 at gmail.com Fri Sep 9 20:13:47 2022 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Fri, 9 Sep 2022 20:13:47 -0500 Subject: [Histonet] Molds- cold vs warm In-Reply-To: References: <40CBA05C-4304-40DA-81A7-BA5901024E04@sbcglobal.net> <29E51433-19F4-420B-B5E4-B098EF4BE6A2@gmail.com> Message-ID: Thank You so much Jay, for such detailed explanation and for permission to use your email to address. My sincere regards, Naira On Fri, Sep 9, 2022 at 5:00 PM Cooper, Brian wrote: > Thanks for saying this Jay!! I have to say, it's been a while since we've > had such a great response on Histonet!! Everything you said is spot on. > > Happy Friday everyone! > > Thanks, > > Brian Cooper > Histology Supervisor > Children's Hospital Los Angeles > Sent from my mobile > > > > > > On Sep 9, 2022 2:37 PM, Jay Lundgren via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** > This email came from outside CHLA. Do not open attachments, click on > links, or respond unless you expected this message and recognize the email > address: histonet-bounces at lists.utsouthwestern.edu. > > > Whoever is telling you to use cold molds needs to go back to clown college. > > That is totally, 100%, absolutely, wrong. > > There is some debate as to embed "wet" (cassettes submerged in paraffin > bath) or "dry", and I will accept either, as mostly a matter of personal > preference. BUT, in both of these cases, the molds are hot. > > I have been a Histotech for five decades, trained at Armed Forces Institute > of Pathology (back when that used to mean something) and I have NEVER seen > anyone using cold molds. > > It is a guaranteed way to get cold fractures and cracks in your blocks, or > to pop tissue out when you are cutting, which might be irretrievable. Just > think how much time all those re-embedded blocks are going to save you! > > Also, you won't be able to easily re-position specimens in the block, to > put them "on edge" or "on end", for example. The tissue will instantly > stick to the cold mold. And if you want to re-position it, guess what, > you'll have to warm the mold up to get the tissue unstuck. How's that > (non-existent anyway) time savings now? > > > If you want to prove to whatever jackass suggested this that they are > wrong, get a big stack of every histopathology textbook you can find. > There is nothing in any of them talking about paraffin embedding with cold > molds. > > As a matter of fact, every single textbook will specify molds at the same > temp as paraffin. > > Anyway, it doesn't even make sense, thermodynamically. Heat travels from > hot to cold. Those "cold" molds will be the same temperature as the > paraffin, almost instantly. Did it take a tiny amount of heat out of the > hot paraffin? Yes, but not enough to noticeably cool the blocks faster. The > amount of heat from the paraffin used to warm the mold is trivial compared > to the total heat of the system. That's why cold plates have huge, noisy > refrigeration units. You can't argue with thermodynamics. > > If you are having trouble getting your blocks to release, use mold > release! Viola! > https://secure-web.cisco.com/1vhENcmRngDgLubdLEYMzzWWUK4ILg_WIJNnMutz67Oikk5LSg5SqF6OvSQqMWpr4MIirbF_ExGbIXm9Usdm35LUk87pXYTIvPVdKY5u2dRCdo_Ss-iuZ4nCOa0nPTIpPec8zwvOBcVIE7eM7o-flt9BAIGK0ZOw4K3HOXwNiLmQBnD0hFb9pgrU0ZuPnk5llOYCeJ5b2Pmkp2B9UPlVvxPMI3-iHRILtOB4kPL45PII_yUJnJhFYAryeid5lrITtm-w0KNyKrfJVI0mHy47Niz0TEpxxvl3DoTDmq-umsyN3BucCj2B-aJFqJ-AW3thtXSEk-Nl0NzBBrSxw8cPzSrKsVww7cCLh_krbh7VXKlRiRGF41o3UKk_oEQuHGIEeYlUNLnpLndnkSH0cwR3nNWhq3Cy8hw6ws0Ka8kYRH8_TVttsOh_lQbO4tm6_i-fdNOZxcR_7t-QeE9aW5YP1hg/https%3A%2F%2Fwww.statlab.com > I used to think it was > superfluous, but now I consider it compulsory. This is probably the answer > to most of your issues. > > I don't know who is suggesting using cold molds, but I can pretty much > guarantee that it's a pathologist who thinks his slides are taking too > long, and knows nothing about histopathology, or a lab manager, who knows > nothing about histopathology. This next part is directly to them. > > To Whoever Suggested Cold Molds: The answer to getting your slides out > quicker is buying more equipment and hiring more techs, and holding > everyone to standards (30 blocks/hr cutting, 60 blocks/hr embedding). > Making nonsensical, uninformed suggestions only exposes your ignorance. > > Please feel free to show them this reply. > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > http://secure-web.cisco.com/1sst_F8q-xPNVIECxucZyjCFTGbDl-OZqoDxUAqSsYz8xAR-kLrSYbAJ1I5oMBLXtOcoOm7CKoMBxRIyL2NOyKolfXTBFuF7FYiWgJio8Ku7hKdyCkzHYmQCcne4XS1yWXzU-X4Szp0h5-1FvqjOhMNcN0drsSahjrT25jvMdlloIXxuy0EcG8_c1lTs0zgq-wevTMHkxwJkdLA8z_JJDz3Kloe4S_3DcwNmZjPIfdHx3BZdIiaKRE3Gauws2kOOKMMztVdzAOkyE-MMMfyehmZCrAryOUn-qd-XRsYHczeIAYf57VjR5N7HwW_sydi3gMX7NUNiR0j5ZI8-xq4pfDE5mhnYScwa1RGqaZ3VVqG5jFDIa0Js84cz0UXMeB7PbQRxosMdOPmtRX9ycvNM1S7iijUEFk5smWvT33f1CHH_9qPWBb9KM0q-faJj10SacllYXV1K3egxhFh1DNkihHw/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, > please contact the sender by reply e-mail and destroy all copies of this > original message. > From tpodawiltz at yahoo.com Fri Sep 9 20:41:35 2022 From: tpodawiltz at yahoo.com (Thomas Podawiltz) Date: Sat, 10 Sep 2022 01:41:35 +0000 (UTC) Subject: [Histonet] Molds- cold vs warm In-Reply-To: References: <40CBA05C-4304-40DA-81A7-BA5901024E04@sbcglobal.net> <29E51433-19F4-420B-B5E4-B098EF4BE6A2@gmail.com> Message-ID: <1899244810.950911.1662774095325@mail.yahoo.com> Tell us how you really feel Jay. LOL.? You say everything that I would say and have said while training people.? Like you having been doing this for a bit (1980) and had ?the privilege of meeting Mr. Lee Luna.? ? Sent from Yahoo Mail for iPad On Friday, September 9, 2022, 6:14 PM, Naira Margaryan via Histonet wrote: Thank You so much Jay, for such detailed explanation and for permission to use your email to address. My sincere regards, Naira On Fri, Sep 9, 2022 at 5:00 PM Cooper, Brian wrote: > Thanks for saying this Jay!! I have to say, it's been a while since we've > had such a great response on Histonet!! Everything you said is spot on. > > Happy Friday everyone! > > Thanks, > > Brian Cooper > Histology Supervisor > Children's Hospital Los Angeles > Sent from my mobile > > > > > > On Sep 9, 2022 2:37 PM, Jay Lundgren via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** > This email came from outside CHLA. Do not open attachments, click on > links, or respond unless you expected this message and recognize the email > address: histonet-bounces at lists.utsouthwestern.edu. > > > Whoever is telling you to use cold molds needs to go back to clown college. > > That is totally, 100%, absolutely, wrong. > > There is some debate as to embed "wet" (cassettes submerged in paraffin > bath) or "dry", and I will accept either, as mostly a matter of personal > preference.? BUT, in both of these cases, the molds are hot. > > I have been a Histotech for five decades, trained at Armed Forces Institute > of Pathology (back when that used to mean something) and I have NEVER seen > anyone using cold molds. > > It is a guaranteed way to get cold fractures and cracks in your blocks, or > to pop tissue out when you are cutting, which might be irretrievable. Just > think how much time all those re-embedded blocks are going to save you! > > Also, you won't be able to easily re-position specimens in the block, to > put them "on edge" or "on end", for example.? The tissue will instantly > stick to the cold mold.? And if you want to re-position it, guess what, > you'll have to warm the mold up to get the tissue unstuck.? How's that > (non-existent anyway) time savings now? > > > If you want to prove to whatever jackass suggested this that they are > wrong, get a big stack of every histopathology textbook you can find. > There is nothing in any of them talking about paraffin embedding with cold > molds. > > As a matter of fact, every single textbook will specify molds at the same > temp as paraffin. > > Anyway, it doesn't even make sense, thermodynamically.? Heat travels from > hot to cold.? Those "cold" molds will be the same temperature as the > paraffin, almost instantly. Did it take a tiny amount of heat out of the > hot paraffin? Yes, but not enough to noticeably cool the blocks faster. The > amount of heat from the paraffin used to warm the mold is trivial compared > to the total heat of the system. That's why cold plates have huge, noisy > refrigeration units.? You can't argue with thermodynamics. > > If you are having trouble getting your blocks to release, use mold > release!? Viola! > https://secure-web.cisco.com/1vhENcmRngDgLubdLEYMzzWWUK4ILg_WIJNnMutz67Oikk5LSg5SqF6OvSQqMWpr4MIirbF_ExGbIXm9Usdm35LUk87pXYTIvPVdKY5u2dRCdo_Ss-iuZ4nCOa0nPTIpPec8zwvOBcVIE7eM7o-flt9BAIGK0ZOw4K3HOXwNiLmQBnD0hFb9pgrU0ZuPnk5llOYCeJ5b2Pmkp2B9UPlVvxPMI3-iHRILtOB4kPL45PII_yUJnJhFYAryeid5lrITtm-w0KNyKrfJVI0mHy47Niz0TEpxxvl3DoTDmq-umsyN3BucCj2B-aJFqJ-AW3thtXSEk-Nl0NzBBrSxw8cPzSrKsVww7cCLh_krbh7VXKlRiRGF41o3UKk_oEQuHGIEeYlUNLnpLndnkSH0cwR3nNWhq3Cy8hw6ws0Ka8kYRH8_TVttsOh_lQbO4tm6_i-fdNOZxcR_7t-QeE9aW5YP1hg/https%3A%2F%2Fwww.statlab.com > I used to think it was > superfluous, but now I consider it compulsory.? This is probably the answer > to most of your issues. > > I don't know who is suggesting using cold molds, but I can pretty much > guarantee that it's a pathologist who thinks his slides are taking too > long, and knows nothing about histopathology, or a lab manager, who knows > nothing about histopathology.? This next part is directly to them. > > To Whoever Suggested Cold Molds:? The answer to getting your slides out > quicker is buying more equipment and hiring more techs, and holding > everyone to standards (30 blocks/hr cutting, 60 blocks/hr embedding). > Making nonsensical, uninformed suggestions only exposes your ignorance. > > Please feel free to show them this reply. > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > http://secure-web.cisco.com/1sst_F8q-xPNVIECxucZyjCFTGbDl-OZqoDxUAqSsYz8xAR-kLrSYbAJ1I5oMBLXtOcoOm7CKoMBxRIyL2NOyKolfXTBFuF7FYiWgJio8Ku7hKdyCkzHYmQCcne4XS1yWXzU-X4Szp0h5-1FvqjOhMNcN0drsSahjrT25jvMdlloIXxuy0EcG8_c1lTs0zgq-wevTMHkxwJkdLA8z_JJDz3Kloe4S_3DcwNmZjPIfdHx3BZdIiaKRE3Gauws2kOOKMMztVdzAOkyE-MMMfyehmZCrAryOUn-qd-XRsYHczeIAYf57VjR5N7HwW_sydi3gMX7NUNiR0j5ZI8-xq4pfDE5mhnYScwa1RGqaZ3VVqG5jFDIa0Js84cz0UXMeB7PbQRxosMdOPmtRX9ycvNM1S7iijUEFk5smWvT33f1CHH_9qPWBb9KM0q-faJj10SacllYXV1K3egxhFh1DNkihHw/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, > please contact the sender by reply e-mail and destroy all copies of this > original message. > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA at mercer.edu Sat Sep 10 08:12:35 2022 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Sat, 10 Sep 2022 13:12:35 +0000 Subject: [Histonet] Molds- cold vs warm In-Reply-To: References: <40CBA05C-4304-40DA-81A7-BA5901024E04@sbcglobal.net> <29E51433-19F4-420B-B5E4-B098EF4BE6A2@gmail.com> Message-ID: Well said Jay. Thanks, Shirley Powell -----Original Message----- From: Jay Lundgren via Histonet Sent: Friday, September 9, 2022 5:37 PM To: Naira Margaryan Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Molds- cold vs warm Whoever is telling you to use cold molds needs to go back to clown college. That is totally, 100%, absolutely, wrong. There is some debate as to embed "wet" (cassettes submerged in paraffin bath) or "dry", and I will accept either, as mostly a matter of personal preference. BUT, in both of these cases, the molds are hot. I have been a Histotech for five decades, trained at Armed Forces Institute of Pathology (back when that used to mean something) and I have NEVER seen anyone using cold molds. It is a guaranteed way to get cold fractures and cracks in your blocks, or to pop tissue out when you are cutting, which might be irretrievable. Just think how much time all those re-embedded blocks are going to save you! Also, you won't be able to easily re-position specimens in the block, to put them "on edge" or "on end", for example. The tissue will instantly stick to the cold mold. And if you want to re-position it, guess what, you'll have to warm the mold up to get the tissue unstuck. How's that (non-existent anyway) time savings now? If you want to prove to whatever jackass suggested this that they are wrong, get a big stack of every histopathology textbook you can find. There is nothing in any of them talking about paraffin embedding with cold molds. As a matter of fact, every single textbook will specify molds at the same temp as paraffin. Anyway, it doesn't even make sense, thermodynamically. Heat travels from hot to cold. Those "cold" molds will be the same temperature as the paraffin, almost instantly. Did it take a tiny amount of heat out of the hot paraffin? Yes, but not enough to noticeably cool the blocks faster. The amount of heat from the paraffin used to warm the mold is trivial compared to the total heat of the system. That's why cold plates have huge, noisy refrigeration units. You can't argue with thermodynamics. If you are having trouble getting your blocks to release, use mold release! Viola! https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.statlab.com%2F&data=05%7C01%7Cpowell_sa%40mercer.edu%7C84d81a9eddad4ba8159308da92ab72a7%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C637983562331339740%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=YDxIy7oMkibqOiMnl0D44LCG3XD0oKPY7jFHZBHBrsM%3D&reserved=0. I used to think it was superfluous, but now I consider it compulsory. This is probably the answer to most of your issues. I don't know who is suggesting using cold molds, but I can pretty much guarantee that it's a pathologist who thinks his slides are taking too long, and knows nothing about histopathology, or a lab manager, who knows nothing about histopathology. This next part is directly to them. To Whoever Suggested Cold Molds: The answer to getting your slides out quicker is buying more equipment and hiring more techs, and holding everyone to standards (30 blocks/hr cutting, 60 blocks/hr embedding). Making nonsensical, uninformed suggestions only exposes your ignorance. Please feel free to show them this reply. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cpowell_sa%40mercer.edu%7C84d81a9eddad4ba8159308da92ab72a7%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C637983562331339740%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=zA5yQmG4SG0GCEs7Wqw%2FUegWBgNiTZl5gVC5EaQRMMI%3D&reserved=0 From Shirley.Ennis at pbrc.edu Sat Sep 10 16:36:34 2022 From: Shirley.Ennis at pbrc.edu (Shirley Ennis) Date: Sat, 10 Sep 2022 21:36:34 +0000 Subject: [Histonet] Molds- cold vs warm In-Reply-To: References: <40CBA05C-4304-40DA-81A7-BA5901024E04@sbcglobal.net> <29E51433-19F4-420B-B5E4-B098EF4BE6A2@gmail.com> Message-ID: Jay ,I totally agree . Shirley Get Outlook for iOS ________________________________ From: Shirley A. Powell via Histonet Sent: Saturday, September 10, 2022 8:12:35 AM To: Jay Lundgren ; Naira Margaryan Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Molds- cold vs warm Well said Jay. Thanks, Shirley Powell -----Original Message----- From: Jay Lundgren via Histonet Sent: Friday, September 9, 2022 5:37 PM To: Naira Margaryan Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Molds- cold vs warm Whoever is telling you to use cold molds needs to go back to clown college. That is totally, 100%, absolutely, wrong. There is some debate as to embed "wet" (cassettes submerged in paraffin bath) or "dry", and I will accept either, as mostly a matter of personal preference. BUT, in both of these cases, the molds are hot. I have been a Histotech for five decades, trained at Armed Forces Institute of Pathology (back when that used to mean something) and I have NEVER seen anyone using cold molds. It is a guaranteed way to get cold fractures and cracks in your blocks, or to pop tissue out when you are cutting, which might be irretrievable. Just think how much time all those re-embedded blocks are going to save you! Also, you won't be able to easily re-position specimens in the block, to put them "on edge" or "on end", for example. The tissue will instantly stick to the cold mold. And if you want to re-position it, guess what, you'll have to warm the mold up to get the tissue unstuck. How's that (non-existent anyway) time savings now? If you want to prove to whatever jackass suggested this that they are wrong, get a big stack of every histopathology textbook you can find. There is nothing in any of them talking about paraffin embedding with cold molds. As a matter of fact, every single textbook will specify molds at the same temp as paraffin. Anyway, it doesn't even make sense, thermodynamically. Heat travels from hot to cold. Those "cold" molds will be the same temperature as the paraffin, almost instantly. Did it take a tiny amount of heat out of the hot paraffin? Yes, but not enough to noticeably cool the blocks faster. The amount of heat from the paraffin used to warm the mold is trivial compared to the total heat of the system. That's why cold plates have huge, noisy refrigeration units. You can't argue with thermodynamics. If you are having trouble getting your blocks to release, use mold release! Viola! https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.statlab.com%2F&data=05%7C01%7Cpowell_sa%40mercer.edu%7C84d81a9eddad4ba8159308da92ab72a7%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C637983562331339740%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=YDxIy7oMkibqOiMnl0D44LCG3XD0oKPY7jFHZBHBrsM%3D&reserved=0. I used to think it was superfluous, but now I consider it compulsory. This is probably the answer to most of your issues. I don't know who is suggesting using cold molds, but I can pretty much guarantee that it's a pathologist who thinks his slides are taking too long, and knows nothing about histopathology, or a lab manager, who knows nothing about histopathology. This next part is directly to them. To Whoever Suggested Cold Molds: The answer to getting your slides out quicker is buying more equipment and hiring more techs, and holding everyone to standards (30 blocks/hr cutting, 60 blocks/hr embedding). Making nonsensical, uninformed suggestions only exposes your ignorance. Please feel free to show them this reply. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cpowell_sa%40mercer.edu%7C84d81a9eddad4ba8159308da92ab72a7%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C637983562331339740%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=zA5yQmG4SG0GCEs7Wqw%2FUegWBgNiTZl5gVC5EaQRMMI%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SThompson4 at sonichealthcareusa.com Sat Sep 10 16:44:53 2022 From: SThompson4 at sonichealthcareusa.com (Stephanie L. Thompson) Date: Sat, 10 Sep 2022 21:44:53 +0000 Subject: [Histonet] Hiring Histotechnicians Message-ID: Sonic Healthcare is currently hiring for Histotechnicians in beautiful Exeter, New Hamphshire. The pay Is great, the staff is wonderful to work with, the beaches and mountains are some of the best in the United States. We have a sign-on bonus and offer relocation. Please reach out to me at sthompson4 at sonichealthcareusa.com. This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From steve8438 at gmail.com Sat Sep 10 17:02:54 2022 From: steve8438 at gmail.com (Steven Mello) Date: Sat, 10 Sep 2022 18:02:54 -0400 Subject: [Histonet] Molds- cold vs warm In-Reply-To: References: Message-ID: Very well stated! I too have been histology for 3 decades an never ever heard of cold mold embedding. Unbelievable!! Thank you Jay for your insight? Steven Mello, BS HT(ASCP) Sent from my iPhone > On Sep 10, 2022, at 5:47 PM, Shirley Ennis via Histonet wrote: > > ? Jay ,I totally agree . > > Shirley > > Get Outlook for iOS > ________________________________ > From: Shirley A. Powell via Histonet > Sent: Saturday, September 10, 2022 8:12:35 AM > To: Jay Lundgren ; Naira Margaryan > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Molds- cold vs warm > > Well said Jay. > Thanks, > Shirley Powell > > > -----Original Message----- > From: Jay Lundgren via Histonet > Sent: Friday, September 9, 2022 5:37 PM > To: Naira Margaryan > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Molds- cold vs warm > > Whoever is telling you to use cold molds needs to go back to clown college. > > That is totally, 100%, absolutely, wrong. > > There is some debate as to embed "wet" (cassettes submerged in paraffin > bath) or "dry", and I will accept either, as mostly a matter of personal preference. BUT, in both of these cases, the molds are hot. > > I have been a Histotech for five decades, trained at Armed Forces Institute of Pathology (back when that used to mean something) and I have NEVER seen anyone using cold molds. > > It is a guaranteed way to get cold fractures and cracks in your blocks, or to pop tissue out when you are cutting, which might be irretrievable. Just think how much time all those re-embedded blocks are going to save you! > > Also, you won't be able to easily re-position specimens in the block, to put them "on edge" or "on end", for example. The tissue will instantly stick to the cold mold. And if you want to re-position it, guess what, you'll have to warm the mold up to get the tissue unstuck. How's that (non-existent anyway) time savings now? > > > If you want to prove to whatever jackass suggested this that they are wrong, get a big stack of every histopathology textbook you can find. > There is nothing in any of them talking about paraffin embedding with cold molds. > > As a matter of fact, every single textbook will specify molds at the same temp as paraffin. > > Anyway, it doesn't even make sense, thermodynamically. Heat travels from hot to cold. Those "cold" molds will be the same temperature as the paraffin, almost instantly. Did it take a tiny amount of heat out of the hot paraffin? Yes, but not enough to noticeably cool the blocks faster. The amount of heat from the paraffin used to warm the mold is trivial compared to the total heat of the system. That's why cold plates have huge, noisy refrigeration units. You can't argue with thermodynamics. > > If you are having trouble getting your blocks to release, use mold release! Viola! https://nam04.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.statlab.com%2F&data=05%7C01%7Cpowell_sa%40mercer.edu%7C84d81a9eddad4ba8159308da92ab72a7%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C637983562331339740%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=YDxIy7oMkibqOiMnl0D44LCG3XD0oKPY7jFHZBHBrsM%3D&reserved=0. I used to think it was superfluous, but now I consider it compulsory. This is probably the answer to most of your issues. > > I don't know who is suggesting using cold molds, but I can pretty much guarantee that it's a pathologist who thinks his slides are taking too long, and knows nothing about histopathology, or a lab manager, who knows nothing about histopathology. This next part is directly to them. > > To Whoever Suggested Cold Molds: The answer to getting your slides out quicker is buying more equipment and hiring more techs, and holding everyone to standards (30 blocks/hr cutting, 60 blocks/hr embedding). > Making nonsensical, uninformed suggestions only exposes your ignorance. > > Please feel free to show them this reply. > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cpowell_sa%40mercer.edu%7C84d81a9eddad4ba8159308da92ab72a7%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C637983562331339740%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=zA5yQmG4SG0GCEs7Wqw%2FUegWBgNiTZl5gVC5EaQRMMI%3D&reserved=0 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann at aol.com Mon Sep 12 10:51:51 2022 From: thisisann at aol.com (Ann Specian) Date: Mon, 12 Sep 2022 15:51:51 +0000 (UTC) Subject: [Histonet] Clarapath SectionStar References: <682079647.1843596.1662997911032.ref@mail.yahoo.com> Message-ID: <682079647.1843596.1662997911032@mail.yahoo.com> Is anyone familiar with this new technology? Sent from the all new AOL app for iOS From amurvosh at advancederm.net Mon Sep 12 13:29:35 2022 From: amurvosh at advancederm.net (Anne Murvosh) Date: Mon, 12 Sep 2022 18:29:35 +0000 Subject: [Histonet] MOHS scheduling Message-ID: I was wondering, for those doing Mohs cases, how far apart you schedule your patients, and if you do them throughout the day or just in am or pm. Thanks Anne Anne Murvosh Histology Technician Three Locations to Serve YOU! Spokane Valley Northside Coeur d'Alene 1807 N Hutchinson Rd 59 E Queen Ave, Ste 102 1700 W. Riverstone Drive Spokane, WA 99212 Spokane, WA 99207 Coeur d'Alene, ID 83814 509-456-7414 Office 509-624-0763 Fax amurvosh at advancederm.net http://www.advancederm.net This electronic transmission and any documents accompanying this electronic transmission may contain information that is confidential and/or legally privileged. The information is intended only for the use of the individual or entity named above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on or regarding the contents of this electronically transmitted information is strictly prohibited. If you have received this e-mail in error, please notify the sender and delete this message immediately. HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return or destruction. From jaylundgren at gmail.com Mon Sep 12 17:10:30 2022 From: jaylundgren at gmail.com (Jay Lundgren) Date: Mon, 12 Sep 2022 17:10:30 -0500 Subject: [Histonet] Clarapath SectionStar In-Reply-To: <682079647.1843596.1662997911032@mail.yahoo.com> References: <682079647.1843596.1662997911032.ref@mail.yahoo.com> <682079647.1843596.1662997911032@mail.yahoo.com> Message-ID: >From their website, hilarious: "Long overdue for a change, the current process is entirely manual, non-scalable, and is dependent on a shrinking workforce. The COVID-19 pandemic has exacerbated labor shortages, creating unacceptable delays in the delivery of timely biopsy results and driving up healthcare costs." When they say "healthcare costs", they mean "Histotech salaries". Remember the story of John Henry? Jay From GKeyser at uwhealth.org Tue Sep 13 07:05:51 2022 From: GKeyser at uwhealth.org (Keyser, Geri L) Date: Tue, 13 Sep 2022 12:05:51 +0000 Subject: [Histonet] MOHS scheduling In-Reply-To: References: Message-ID: My institution schedules them 15 min apart (with an hour and a half break between 8:30AM and 10:00AM). We typically do 7 cases per day, per physician. Up to 2 physicians. Maybe two multi-site cases per physician. But, we have a fairly large clinic with a significant clinic staff and two to three techs (Three available workstations) working the lab in order to make this workload viable. I know many clinics are smaller operations. The early afternoon is used for closures and finishing up the procedures that haven't cleared yet. The rest of the day is used for closures. I don't know what is typical. I've been here for 15 years and have no experience with how other clinics do things. I'm very interested to hear what other clinics look like. ? Geri -----Original Message----- From: Anne Murvosh via Histonet Sent: Monday, September 12, 2022 1:30 PM To: histonet (histonet at lists.utsouthwestern.edu) Subject: [Histonet] MOHS scheduling WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. I was wondering, for those doing Mohs cases, how far apart you schedule your patients, and if you do them throughout the day or just in am or pm. Thanks Anne Anne Murvosh Histology Technician Three Locations to Serve YOU! Spokane Valley Northside Coeur d'Alene 1807 N Hutchinson Rd 59 E Queen Ave, Ste 102 1700 W. Riverstone Drive Spokane, WA 99212 Spokane, WA 99207 Coeur d'Alene, ID 83814 509-456-7414 Office 509-624-0763 Fax amurvosh at advancederm.net https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.advancederm.net%2F&data=05%7C01%7CGKeyser%40uwhealth.org%7Cd1e904baf325436f60d108da94eccf4e%7C0fd7902a3b4f49b0b1edaaa4d2b4f5f1%7C0%7C0%7C637986042059330692%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=9RJ4GGdIaZcq%2FuJQEeR%2FDj3nkMZ8jYjBs5ydVkkyNKE%3D&reserved=0 This electronic transmission and any documents accompanying this electronic transmission may contain information that is confidential and/or legally privileged. The information is intended only for the use of the individual or entity named above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on or regarding the contents of this electronically transmitted information is strictly prohibited. If you have received this e-mail in error, please notify the sender and delete this message immediately. HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return or destruction. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7CGKeyser%40uwhealth.org%7Cd1e904baf325436f60d108da94eccf4e%7C0fd7902a3b4f49b0b1edaaa4d2b4f5f1%7C0%7C0%7C637986042059486932%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=E%2F%2FCEJSNwiEa%2BL0e89qJvseFAvKcEuTgi5OblAIGO2g%3D&reserved=0 From tbraud at holyredeemer.com Tue Sep 13 16:03:21 2022 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 13 Sep 2022 21:03:21 +0000 Subject: [Histonet] Clarapath SectionStar Message-ID: <71a9173239ac428aad564087ef79a313@holyredeemer.com> I'm not holding my breath for this one. Not to say it will never happen, but don't look for this one anytime in the foreseeable future. Terri Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion From jaylundgren at gmail.com Tue Sep 13 16:47:34 2022 From: jaylundgren at gmail.com (Jay Lundgren) Date: Tue, 13 Sep 2022 16:47:34 -0500 Subject: [Histonet] Clarapath SectionStar In-Reply-To: <71a9173239ac428aad564087ef79a313@holyredeemer.com> References: <71a9173239ac428aad564087ef79a313@holyredeemer.com> Message-ID: If it does come out, it'll probably cost $9,000,000.00 anyway. A lot of places won't be able to afford it. Anyway, research scientists are making incredible advances in diagnosing cancer with circulating cancer dna blood testing. They can even tell if the tumor is primary or metastatic through a blood test now! Maybe that will make glass slides obsolete before they can get this thing to market. It's interesting though. We're at the stage in robotics roughly equivalent to the 1940s computer. In the 1940s, you would build a computer for one thing. If you wanted a range finder, you built a range finding computer. If you wanted a decoder, you built a decoding computer. Then the concept of one computer that could do many different tasks with different software came along. So now, you can decode, play games, communicate, edit video, etc., All with the same computer. Right now, most of the robots in the world are either welders or painters in car factories, some vacuum floors. Here's a robot to section paraffin blocks. Single purpose. But soon, sooner than we think, probably, we will have a single robot that can weld your car, vacuum your floor, section your blocks, cook you dinner in a Michelin star restaurant, and do heart surgery much better than any human surgeon. Then, there won't be any need for histotechs. Or welders, or surgeons, or chefs, or... Jay From Jose.R.deGuzman at gunet.georgetown.edu Thu Sep 15 08:45:58 2022 From: Jose.R.deGuzman at gunet.georgetown.edu (De Guzman, Jose V) Date: Thu, 15 Sep 2022 13:45:58 +0000 Subject: [Histonet] Molds- cold vs warm Message-ID: Hi Naira, If removing the blocks from the molds and removing the excess paraffin is taking too long, let's take a look at your equipment then technique. 1. How cold does the cold plate get? 2. How fast does it reach freezing temperature? 3. Does it maintain that same temperature the entire time spent embedding? Technique helps with how much force is needed to remove the block from the mold. Some need to pry the block while others can embed and the mold comes off easily. Technique also helps reduce the amount of excess paraffin you need to remove from the sides. If you have a Wax Trimmer or looking to get one, choose one with a higher working temperature. While equipment and technique can save time, goals and targets should be reasonable. How much time are you actually spending performing these tasks? Jose From plucas at biopath.org Thu Sep 22 10:55:33 2022 From: plucas at biopath.org (Paula) Date: Thu, 22 Sep 2022 08:55:33 -0700 Subject: [Histonet] Leica Prisma problem Message-ID: <003f01d8ce9b$c0dc85c0$42959140$@biopath.org> Hello everyone, We have the Leica Prisma film coverslipper and suddenly, there are bubbles underneath the film. I changed out the Xylene and inserted a new film roll but the problem persists. Is there anything that you recommend to solve the problem? Thank you in advance, Paula Lucas Lab Manager Bio-Path Medical Group From plucas at biopath.org Thu Sep 22 11:46:08 2022 From: plucas at biopath.org (Paula) Date: Thu, 22 Sep 2022 09:46:08 -0700 Subject: [Histonet] Leica Prisma problem In-Reply-To: <767eefa4e25a4911bca3f21f7dfe3ae2@RWJBH.org> References: <003f01d8ce9b$c0dc85c0$42959140$@biopath.org> <767eefa4e25a4911bca3f21f7dfe3ae2@RWJBH.org> Message-ID: <004b01d8cea2$d1e2dfc0$75a89f40$@biopath.org> Hi Toni, Yes, I'm sorry..it is a Sakura product. I have called tech support and they want to visit but hoping I can fix it myself quicker if it's a simple fix. Thanks, Paula -----Original Message----- From: Rathborne, Toni [mailto:Toni.Rathborne at RWJBH.org] Sent: Thursday, September 22, 2022 9:36 AM To: 'Paula' Subject: RE: [Histonet] Leica Prisma problem Paula, Isn't the Prisma a Sakura product? Have you called the vendor to ask for technical support over the phone? They are usually good about doing this, even if you don't have a service contract. You should also mention the lot number of the tape that you're using so they can document and track the problem if it has happened to other users. Toni Rathborne Pathology Supervisor Robert Wood Johnson University Hospital Somerset ------------------------------------------------------------------------------------ NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. -----Original Message----- From: Paula via Histonet Sent: Thursday, September 22, 2022 11:56 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Leica Prisma problem *** This is an External Email *** Hello everyone, We have the Leica Prisma film coverslipper and suddenly, there are bubbles underneath the film. I changed out the Xylene and inserted a new film roll but the problem persists. Is there anything that you recommend to solve the problem? Thank you in advance, Paula Lucas Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KU82p_qNqnQ!62MqADzSvCmn3BCClnb5s2_N3knTdw5FcHUsNGAHIOWQ9bhLFblOr-VTHzGGLyzIoxodLS-sW6xxh1lBS5Plhe6FKawFf-i9BDc$ From SThompson4 at sonichealthcareusa.com Thu Sep 22 12:08:16 2022 From: SThompson4 at sonichealthcareusa.com (Stephanie L. Thompson) Date: Thu, 22 Sep 2022 17:08:16 +0000 Subject: [Histonet] Jobs Available Message-ID: <72acdec52f374d4fb9d3cb369d3e67bd@sonichealthcareusa.com> We have Lead and Supervisory Histotechnician positions open in several states. We are hiring in Exeter, New Hampshire, it is a beautiful place to live, the beaches are about an hour away, and the white mountains are amazing! If you, or someone you know, is interested in learning more about positions visit our website at www.sonichealthcareusa.com. Or send me your resume to sthompson4 at sonichealthcareusa.com. This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From travis at imebinc.com Thu Sep 22 13:00:57 2022 From: travis at imebinc.com (travis at imebinc.com) Date: Thu, 22 Sep 2022 11:00:57 -0700 Subject: [Histonet] Leica Prisma problem In-Reply-To: <003f01d8ce9b$c0dc85c0$42959140$@biopath.org> References: <003f01d8ce9b$c0dc85c0$42959140$@biopath.org> Message-ID: <04d001d8cead$47014800$d503d800$@imebinc.com> Hello, Assuming you are staining correctly, this issue most likely isn?t the coverslipper itself. If the coverslipper is clean it should preform correctly. The issue most likely stems from the roll of tape (Part# 4770). It is a very hot time of the year, so make sure you are following all the rules of Sakura's tape. Take note of the 24 hour rule on the back of 4770 box ,"Allow the film to completely acclimate to the environment in which it will be used. The film package should be opened and stored in a dust-free environment and a temperature range (50 - 86 F) and humidity levels between 30% - 70% for at least 24 hours prior to use." Also, if the tape is exposed to temps over 104 F, it could ruin the product. Travis O?Brien ? International Medical Equipment, Inc. 170 Vallecitos De Oro San Marcos, CA 92069 ? 800.543.8496? Phone 760.761.0859? Fax www.imebinc.com -----Original Message----- From: Paula via Histonet Sent: Thursday, September 22, 2022 8:56 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Leica Prisma problem Hello everyone, We have the Leica Prisma film coverslipper and suddenly, there are bubbles underneath the film. I changed out the Xylene and inserted a new film roll but the problem persists. Is there anything that you recommend to solve the problem? Thank you in advance, Paula Lucas Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From penny.marr at nhs.net Fri Sep 23 01:51:09 2022 From: penny.marr at nhs.net (MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST)) Date: Fri, 23 Sep 2022 06:51:09 +0000 Subject: [Histonet] Leica Prisma problem In-Reply-To: <004b01d8cea2$d1e2dfc0$75a89f40$@biopath.org> References: <003f01d8ce9b$c0dc85c0$42959140$@biopath.org> <767eefa4e25a4911bca3f21f7dfe3ae2@RWJBH.org> <004b01d8cea2$d1e2dfc0$75a89f40$@biopath.org> Message-ID: I suggest an extended prime to ensure no air in the line then consider if more drops of xylene are needed on the slides. Hope this helps. Penny :) Penny Marr Senior BMS C/- Histology Conquest Hospital St Leonards-on-Sea TN37 7RD penny.marr at nhs.net 0300 131 4500 ext 734914 0300 131 4914 -----Original Message----- From: Paula Sent: 22 September 2022 17:46 To: 'Rathborne, Toni' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Leica Prisma problem Hi Toni, Yes, I'm sorry..it is a Sakura product. I have called tech support and they want to visit but hoping I can fix it myself quicker if it's a simple fix. Thanks, Paula -----Original Message----- From: Rathborne, Toni [mailto:Toni.Rathborne at RWJBH.org] Sent: Thursday, September 22, 2022 9:36 AM To: 'Paula' Subject: RE: [Histonet] Leica Prisma problem Paula, Isn't the Prisma a Sakura product? Have you called the vendor to ask for technical support over the phone? They are usually good about doing this, even if you don't have a service contract. You should also mention the lot number of the tape that you're using so they can document and track the problem if it has happened to other users. Toni Rathborne Pathology Supervisor Robert Wood Johnson University Hospital Somerset ------------------------------------------------------------------------------------ NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. -----Original Message----- From: Paula via Histonet Sent: Thursday, September 22, 2022 11:56 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Leica Prisma problem *** This is an External Email *** Hello everyone, We have the Leica Prisma film coverslipper and suddenly, there are bubbles underneath the film. I changed out the Xylene and inserted a new film roll but the problem persists. Is there anything that you recommend to solve the problem? Thank you in advance, Paula Lucas Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KU82p_qNqnQ!62MqADzSvCmn3BCClnb5s2_N3knTdw5FcHUsNGAHIOWQ9bhLFblOr-VTHzGGLyzIoxodLS-sW6xxh1lBS5Plhe6FKawFf-i9BDc$ ************************************************************************************** ****************************** This message may contain confidential information. If you are not the intended recipient please: i) inform the sender that you have received the message in error before deleting it; and ii) do not disclose, copy or distribute information in this e-mail or take any action in relation to its content (to do so is strictly prohibited and may be unlawful). Thank you for your co-operation. NHSmail is the secure email, collaboration and directory service available for all NHS staff in England. NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and other accredited email services. For more information and to find out how you can switch visit Joining NHSmail ? NHSmail Support From TNMayer at mdanderson.org Fri Sep 23 11:09:28 2022 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Fri, 23 Sep 2022 16:09:28 +0000 Subject: [Histonet] Leica Prisma problem Message-ID: I would check the roller to see if it is making good contact and the slide holder track as well. Not sure it that is what it is called, but where the slide is pushed under the roller. Sincerely, Toysha N. Mayer, DHSc, MBA, HT (ASCP) Assistant Professor/Associate Program Director HTL Program School of Health Professions MD Anderson Cancer Center tnmayer at mdanderson.org 713-563-3481 wk 832-710-1837 cell Message: 1 Date: Thu, 22 Sep 2022 08:55:33 -0700 From: "Paula" To: Subject: [Histonet] Leica Prisma problem Message-ID: <003f01d8ce9b$c0dc85c0$42959140$@biopath.org> Content-Type: text/plain; charset="us-ascii" Hello everyone, We have the Leica Prisma film coverslipper and suddenly, there are bubbles underneath the film. I changed out the Xylene and inserted a new film roll but the problem persists. Is there anything that you recommend to solve the problem? Thank you in advance, Paula Lucas Lab Manager Bio-Path Medical Group ------------------------------ ***************************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From Emmanuel-Geoffre.S.Gonzaga at kp.org Fri Sep 23 12:22:49 2022 From: Emmanuel-Geoffre.S.Gonzaga at kp.org (Emmanuel Geoffrey S. Gonzaga) Date: Fri, 23 Sep 2022 17:22:49 +0000 Subject: [Histonet] Leica Prisma problem In-Reply-To: References: Message-ID: In addition to the roller, make sure to check the springs that hold the rollers. -------- Emmanuel Gonzaga Operations Manager (Histology/IHC) Kaiser Permanente Histology Department- Chino Hills 13000 Peyton Drive Chino Hills, CA 91709 (909) 703-6931 (Office) / 263 (tie-line) (909) 703-6924 (Lab) (909) 703-6080 (Fax) --------- kp.org/thrive Upcoming PTO: -----Original Message----- From: Mayer,Toysha N via Histonet Sent: Friday, September 23, 2022 9:09 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Leica Prisma problem Caution: This email came from outside Kaiser Permanente. Do not open attachments or click on links if you do not recognize the sender. ______________________________________________________________________ I would check the roller to see if it is making good contact and the slide holder track as well. Not sure it that is what it is called, but where the slide is pushed under the roller. Sincerely, Toysha N. Mayer, DHSc, MBA, HT (ASCP) Assistant Professor/Associate Program Director HTL Program School of Health Professions MD Anderson Cancer Center tnmayer at mdanderson.org 713-563-3481 wk 832-710-1837 cell Message: 1 Date: Thu, 22 Sep 2022 08:55:33 -0700 From: "Paula" To: Subject: [Histonet] Leica Prisma problem Message-ID: <003f01d8ce9b$c0dc85c0$42959140$@biopath.org> Content-Type: text/plain; charset="us-ascii" Hello everyone, We have the Leica Prisma film coverslipper and suddenly, there are bubbles underneath the film. I changed out the Xylene and inserted a new film roll but the problem persists. Is there anything that you recommend to solve the problem? Thank you in advance, Paula Lucas Lab Manager Bio-Path Medical Group ------------------------------ ***************************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!BZ50a36bapWJ!qCRq0Sr10bKrOt35ujh73e-4qe5QwxEycc_CyW2Qf_pL8-v-5YNPYKW33hFv62b9Ammamyc54g-Az-_i0EQny7TjCfWaisfRNAb9Kg5AL0QL$ NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. v.173.295 Thank you. From criley at udel.edu Fri Sep 23 12:44:07 2022 From: criley at udel.edu (Charles Riley) Date: Fri, 23 Sep 2022 13:44:07 -0400 Subject: [Histonet] Manual IHC staining help Message-ID: I have worked mostly with pre-built kits for automated platforms. I am in the process of doing studies on rat tendons using IL-6, MMP3, Collagen I, and CD68 antibodies. I am purchasing all Rabbit polyclonal antibodies from Invitrogen/ThermoScientific. Can anyone provide me with protocols they use or suggestions as to what other reagents/antibodies I need to buy to get good quality results (i.e. secondary antibodies, blocking buffers, antigen retrieval methods, HRP or streptavidin/biotin conjugates, and or chromagens)? From patpxs at gmail.com Sat Sep 24 11:54:10 2022 From: patpxs at gmail.com (Paula Sicurello) Date: Sat, 24 Sep 2022 16:54:10 +0000 (UTC) Subject: [Histonet] QIHC certification References: <2048790689.182813.1664038450344.ref@mail.yahoo.com> Message-ID: <2048790689.182813.1664038450344@mail.yahoo.com> Hello Listers, Happy Saturday, it's a beautiful day here in San Diego. I have a question:? Is there an alternative route for the experience requirement for the QIHC???I need to get certified but I haven't been doing IHC at my new job.? I have been doing IHC since the early 1990's (remember the VectaStain ABC kits?).? At my prior gig I did manual IF on skin, kidneys (direct), and heart biopsies (indirect). I don't have enough time to work the required amount at my new job.? I can't get a letter from my previous supervisor (she left for greener pastures). Suggestions anybody? Thanks oodles. Sincerely, Paula Sicurello From Nancy.Schmitt at mercyhealth.com Sun Sep 25 15:55:27 2022 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Sun, 25 Sep 2022 20:55:27 +0000 Subject: [Histonet] pathologist competency Message-ID: Hello- Working with our Paths to update their competency policy - including the usual Gyn and Non gyn and surgical surveys along with their interdepartmental documentation; what are other folks including in this? Thank much, Nancy Pathology Support Services Supervisor Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From mcokertx at gmail.com Mon Sep 26 11:28:10 2022 From: mcokertx at gmail.com (Michelle Bell) Date: Mon, 26 Sep 2022 16:28:10 +0000 Subject: [Histonet] pathologist competency In-Reply-To: References: Message-ID: We included random reviews along with any discrepancies and how they were handled. If you look through the CLIA brochure for competency assessments, it defines all of the required elements. You need to cover each of the points mentioned somehow. I included the link :) https://www.cms.gov/regulations-and-guidance/legislation/clia/downloads/clia_compbrochure_508.pdf ________________________________ From: Nancy Schmitt via Histonet Sent: Sunday, September 25, 2022 3:55:27 PM To: Histonet Subject: [Histonet] pathologist competency Hello- Working with our Paths to update their competency policy - including the usual Gyn and Non gyn and surgical surveys along with their interdepartmental documentation; what are other folks including in this? Thank much, Nancy Pathology Support Services Supervisor Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Mon Sep 26 12:15:37 2022 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 26 Sep 2022 17:15:37 +0000 Subject: [Histonet] Pathology Competency Message-ID: <9824db49abda4558a840e14f936114db@holyredeemer.com> Besides the GYN reviews, we use our CAP surveys and circulate the ER/PR, Her2, CD20, Ki-67, and MMR panel between all pathologists for a blind read. The original survey is reported in rotation, then all other pathologists are given blank copies of the answer sheet and asked to fill it out. Then a comparison is made and the results reported, reconciled, and discussed and through the quarterly departmental QA meeting. This was suggested to me by CAP to meet their requirements. Additionally, the pathologists participate in others such as PIP, Non-Gyn Cyt, FNA-G, and of course, MK. I hope that helps. Terri Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion Today's Topics: 1. pathologist competency (Nancy Schmitt) 2. Re: pathologist competency (Michelle Bell) ---------------------------------------------------------------------- Message: 1 Date: Sun, 25 Sep 2022 20:55:27 +0000 From: Nancy Schmitt To: Histonet Subject: [Histonet] pathologist competency Hello- Working with our Paths to update their competency policy - including the usual Gyn and Non gyn and surgical surveys along with their interdepartmental documentation; what are other folks including in this? Thank much, Nancy Pathology Support Services Supervisor From bcooper at chla.usc.edu Wed Sep 28 14:27:38 2022 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 28 Sep 2022 19:27:38 +0000 Subject: [Histonet] Epon Resin Embedding Question Message-ID: <3bc9e59575f24a7abdd16cae92be48f5@chla.usc.edu> Good afternoon Histonet, Asking this question for a friend: Is there a way to correct an improperly prepared Epon resin embedded block? Following embedding, the block is soft and "tacky" to the touch. This block is proving VERY difficult to section. Any assistance from those of you doing EM in your labs would be greatly appreciated. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From patpxs at gmail.com Wed Sep 28 22:28:01 2022 From: patpxs at gmail.com (Paula Sicurello) Date: Thu, 29 Sep 2022 03:28:01 +0000 (UTC) Subject: [Histonet] Epon Resin Embedding Question In-Reply-To: <3bc9e59575f24a7abdd16cae92be48f5@chla.usc.edu> References: <3bc9e59575f24a7abdd16cae92be48f5@chla.usc.edu> Message-ID: <453034136.2224651.1664422081604@mail.yahoo.com> Hi Brian,Unfortunately, no.? The most you can do is to put it back in the oven and let it bake forva very long time. You won't be able to section it.? It will gum up the knife edge.? If they are using a diamond knife they need to clean it right away. To add insult to injury, the poorly polymerized blocks are a safety hazard.? ?Epoxy resins are all toxic, carcinogenic, mutagenic, a reproductive hazard, or all of the above combined.? It?s only when the resin is fully polymerized that it becomes an inert plastic. Perhaps someone else out in Histoland (Tim Morkin, are you out there?) has had luck dealing with blocks like those. Sincerely, Paula Sicurello On Wed, Sep 28, 2022 at 12:27 PM, Cooper, Brian via Histonet wrote: Good afternoon Histonet, Asking this question for a friend:? Is there a way to correct an improperly prepared Epon resin embedded block?? Following embedding, the block is soft and "tacky" to the touch.? This block is proving VERY difficult to section.? Any assistance from those of you doing EM in your labs would be greatly appreciated. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message.? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From duraine at bcm.edu Thu Sep 29 02:49:17 2022 From: duraine at bcm.edu (Duraine, Lita R) Date: Thu, 29 Sep 2022 07:49:17 +0000 Subject: [Histonet] Epon Resin Embedding Question Message-ID: Hi Brian, I regularly use epon for TEM work. Even though the instructions are good for some climates , epon is particular. Here in Houston, I let my blocks cure for 5 to 7 days depending on how humid or rainy it is. When I press a fingernail into the block , if it leaves a mark I know it needs a day or two more. I generally cure at 62 degrees Celsius. Dryer climates you can get away with shorter cure times. Just do the fingernail test before starting to section. If your blocks were in the oven for that many days already, then one of the components of the epon resin was no good or contaminated. If that is so you may salvage your specimen by going backwards in a few steps in propylene oxide, or whatever other dehydration solvent you used before trying to cure in epon. After you get your sample loose then continue forward with new epon, ddsa, nma, and the harder dmp-30. Regards, Lita Duraine Baylor College of Medicine NRI TEM Core 1250 Moursund St. Houston, Tx 77030 Sent from my Verizon, Samsung Galaxy smartphone -------- Original message -------- From: Paula Sicurello via Histonet Date: 9/28/22 10:29 PM (GMT-06:00) To: "Cooper, Brian" , "Cooper, Brian via Histonet" Cc: "Navarro, Alexander" Subject: Re: [Histonet] Epon Resin Embedding Question Hi Brian,Unfortunately, no. The most you can do is to put it back in the oven and let it bake forva very long time. You won't be able to section it. It will gum up the knife edge. If they are using a diamond knife they need to clean it right away. To add insult to injury, the poorly polymerized blocks are a safety hazard. Epoxy resins are all toxic, carcinogenic, mutagenic, a reproductive hazard, or all of the above combined. It?s only when the resin is fully polymerized that it becomes an inert plastic. Perhaps someone else out in Histoland (Tim Morkin, are you out there?) has had luck dealing with blocks like those. Sincerely, Paula Sicurello On Wed, Sep 28, 2022 at 12:27 PM, Cooper, Brian via Histonet wrote: Good afternoon Histonet, Asking this question for a friend: Is there a way to correct an improperly prepared Epon resin embedded block? Following embedding, the block is soft and "tacky" to the touch. This block is proving VERY difficult to section. Any assistance from those of you doing EM in your labs would be greatly appreciated. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Z-Quiwr_oQfGYLNUELwQ1A&m=pOUurHsVwv9Epol1Az0hHfhcrsBosmSdK2lBm64-Gm6FG5QcsL0fVfOTeIEel6cX&s=T-uEszcXUD8mlgTt_28n117WyuHtFJs8AADOpJ-5vyg&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Z-Quiwr_oQfGYLNUELwQ1A&m=pOUurHsVwv9Epol1Az0hHfhcrsBosmSdK2lBm64-Gm6FG5QcsL0fVfOTeIEel6cX&s=T-uEszcXUD8mlgTt_28n117WyuHtFJs8AADOpJ-5vyg&e= From plucas at biopath.org Thu Sep 29 13:59:36 2022 From: plucas at biopath.org (Paula) Date: Thu, 29 Sep 2022 11:59:36 -0700 Subject: [Histonet] IHC background Message-ID: <005601d8d435$9f7d3dd0$de77b970$@biopath.org> Hello everyone, We have the Bond III and we ran an S100. We placed 2 serial sections vertically on the slide with the control tissue at the top for staining. The patient's section at the top underneath the control tissue had background staining, while the section on the bottom of that had no background staining. Can someone determine why this would happen? Thank you in advance. Paula Bio-Path Medical Group From rbasilio9 at sbcglobal.net Thu Sep 29 14:21:46 2022 From: rbasilio9 at sbcglobal.net (RENE MORALES) Date: Thu, 29 Sep 2022 12:21:46 -0700 Subject: [Histonet] IHC background In-Reply-To: <005601d8d435$9f7d3dd0$de77b970$@biopath.org> References: <005601d8d435$9f7d3dd0$de77b970$@biopath.org> Message-ID: <50C0494C-0E38-43A9-8373-F006D1CAB819@sbcglobal.net> Paula, Regardless of the position of the tissue: control/ patient ?s tissue, most likely is the distribution of the reagents during staining, isn?t adequate. Syringe, staining reagents expiration, calibration, etc., Have you contacted service from Leica? Hope the solution is found, Thanks, Sent from my iPhone > On Sep 29, 2022, at 12:00, Paula via Histonet wrote: > > ?Hello everyone, > > > > We have the Bond III and we ran an S100. We placed 2 serial sections > vertically on the slide with the control tissue at the top for staining. The > patient's section at the top underneath the control tissue had background > staining, while the section on the bottom of that had no background > staining. Can someone determine why this would happen? > > > > Thank you in advance. > > Paula > > Bio-Path Medical Group > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From regan.fulton at gmail.com Thu Sep 29 14:42:11 2022 From: regan.fulton at gmail.com (Regan Fulton) Date: Thu, 29 Sep 2022 12:42:11 -0700 Subject: [Histonet] IHC background In-Reply-To: <50C0494C-0E38-43A9-8373-F006D1CAB819@sbcglobal.net> References: <005601d8d435$9f7d3dd0$de77b970$@biopath.org> <50C0494C-0E38-43A9-8373-F006D1CAB819@sbcglobal.net> Message-ID: Hi, The artifact you are seeing is a well-known artifact of the Leica covertile design. It does limit the available footprint for placing samples. Hope this helps. Regan Regan Fulton, M.D., Ph.D. CEO and Co-Founder Array Science, LLC 475 Gate 5 Road, #100 Sausalito, CA 94965 (415) 577-7360 www.arrayscience.com On Thu, Sep 29, 2022 at 12:37 PM RENE MORALES via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Paula, > Regardless of the position of the tissue: control/ patient ?s tissue, most > likely is the distribution of the reagents during staining, isn?t > adequate. Syringe, staining reagents expiration, calibration, etc., Have > you contacted service from Leica? Hope the solution is found, > Thanks, > > > Sent from my iPhone > > > On Sep 29, 2022, at 12:00, Paula via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > > ?Hello everyone, > > > > > > > > We have the Bond III and we ran an S100. We placed 2 serial sections > > vertically on the slide with the control tissue at the top for staining. > The > > patient's section at the top underneath the control tissue had background > > staining, while the section on the bottom of that had no background > > staining. Can someone determine why this would happen? > > > > > > > > Thank you in advance. > > > > Paula > > > > Bio-Path Medical Group > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Toni.Rathborne at RWJBH.org Thu Sep 29 14:54:50 2022 From: Toni.Rathborne at RWJBH.org (Rathborne, Toni) Date: Thu, 29 Sep 2022 19:54:50 +0000 Subject: [Histonet] IHC background In-Reply-To: References: <005601d8d435$9f7d3dd0$de77b970$@biopath.org> <50C0494C-0E38-43A9-8373-F006D1CAB819@sbcglobal.net> Message-ID: We have not encountered a covertile artifact with our Bond III, which we've had for over 10 years. It may be that the covertiles need to be replaced or cleaned more, but we have not experienced this. We also place a control tissue at the top of the slide, and often have large patient sections on the lower portion. Rene had some good suggestions. I would also call Leica to see if they can offer suggestions over the phone. Their technical support team has been very helpful over the years. Best of luck. ------------------------------------------------------------------------------------ NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. -----Original Message----- From: Regan Fulton via Histonet Sent: Thursday, September 29, 2022 3:42 PM To: RENE MORALES Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC background *** This is an External Email *** Hi, The artifact you are seeing is a well-known artifact of the Leica covertile design. It does limit the available footprint for placing samples. Hope this helps. Regan Regan Fulton, M.D., Ph.D. CEO and Co-Founder Array Science, LLC 475 Gate 5 Road, #100 Sausalito, CA 94965 (415) 577-7360 https://urldefense.com/v3/__http://www.arrayscience.com__;!!KU82p_qNqnQ!5wEmn3ZITfdL3bAIxqNnFTc42PyJck0rCawxWamgiaOTD5C-jR4wFY-vcvkycNgcEj5SqBCnxsZ_MciXREnRXzkpNiFxiUj3lZA$ On Thu, Sep 29, 2022 at 12:37 PM RENE MORALES via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Paula, > Regardless of the position of the tissue: control/ patient ?s tissue, most > likely is the distribution of the reagents during staining, isn?t > adequate. Syringe, staining reagents expiration, calibration, etc., Have > you contacted service from Leica? Hope the solution is found, > Thanks, > > > Sent from my iPhone > > > On Sep 29, 2022, at 12:00, Paula via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > > ?Hello everyone, > > > > > > > > We have the Bond III and we ran an S100. We placed 2 serial sections > > vertically on the slide with the control tissue at the top for staining. > The > > patient's section at the top underneath the control tissue had background > > staining, while the section on the bottom of that had no background > > staining. Can someone determine why this would happen? > > > > > > > > Thank you in advance. > > > > Paula > > > > Bio-Path Medical Group > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KU82p_qNqnQ!5wEmn3ZITfdL3bAIxqNnFTc42PyJck0rCawxWamgiaOTD5C-jR4wFY-vcvkycNgcEj5SqBCnxsZ_MciXREnRXzkpNiFxChzOwPk$ > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KU82p_qNqnQ!5wEmn3ZITfdL3bAIxqNnFTc42PyJck0rCawxWamgiaOTD5C-jR4wFY-vcvkycNgcEj5SqBCnxsZ_MciXREnRXzkpNiFxChzOwPk$ > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KU82p_qNqnQ!5wEmn3ZITfdL3bAIxqNnFTc42PyJck0rCawxWamgiaOTD5C-jR4wFY-vcvkycNgcEj5SqBCnxsZ_MciXREnRXzkpNiFxChzOwPk$