[Histonet] Immunofluorescense staining

[Reuel Cornelia]

I just want to ask about precipitation reaction of secondary antibody especially on goat antibody conjugate Alexa dye 594 and 488 Invitrogen. I have noticed that the method I used using 1% BSA and 3% BSA blocking , then overnight with primary
has reacted to my secondary goat anti Rabbit and mouse alexa dyre 594 and 488 with too much precipitation. I tried to remove it with PBS with 0.05 tween wash overnight and the precipitate are still there. Any help would be appreciated with my technique. Thank you so much.

Reuel Cornelia
Cellular Pathology
Laboratory Supervisor
Scottish Rite Hospital for Children
2222 Welborn Street
Dallas, TX 75219
Tel. Number: 214-559-7766
FAX Number: 214-347-4806

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