From amurvosh at advancederm.net Tue Mar 1 09:49:04 2022 From: amurvosh at advancederm.net (Anne Murvosh) Date: Tue, 1 Mar 2022 15:49:04 +0000 Subject: [Histonet] Once-a-month job opportunity Message-ID: We have a position available that is just once-a-month in Walla Walla, Washington. This is a good position for someone retired or free on a Friday who wants some extra cash and lives in Walla Walla or nearby Tri-cities Washington. The hours are 7:30 am to when Mohs is completed between 12-1:00 pm.Two years' experience preferable. Thanks Anne Murvosh H. Please Contact: Advanced Dermatology & Skin Surgery, in connection with United Derm Partners Mohs TECHNICIAN-WALLA WALLA, Washington 3RD Friday of every month Please contact: Anne Marie Poole APoole at Advancederm.net HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. 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From carl.hobbs at kcl.ac.uk Tue Mar 1 13:29:41 2022 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Tue, 1 Mar 2022 19:29:41 +0000 Subject: [Histonet] IHC staining of cartilage Message-ID: Yes...a big problem for me, too....if you are using HIER?? Cartilage and bone are major tissue retention problems I cut Pwax sections of mouse long bones/knee joints for anti GFP IHC I still get a % fall off but, using manual HIER at 90C I manage to get enough sections to get good results However, I imagine that your autoIHC stainer prob uses 90C HIER? I find Trajan 3 series slides more reliable than Superfrost Plus slides. Sure, I need to dry my slides for 24 hrs in an operating fume hood ( we can't turn ours off/down) Then I place slides in a 60C oven for 2hrs. Then dewax etc.... Be interesting to read of other suggestions Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6810 From regan.fulton at gmail.com Tue Mar 1 13:36:11 2022 From: regan.fulton at gmail.com (Regan Fulton) Date: Tue, 1 Mar 2022 11:36:11 -0800 Subject: [Histonet] IHC staining of cartilage In-Reply-To: References: Message-ID: Hi, With regard to adhesion, we find TOMO slides are the best among many that we have tried. Interestingly, a creative histotech I once worked with used a diluted Gorilla Glue smear on the slides just prior to picking up a cartilage section. It was tricky to get the section on while the glue was in a workable condition, but the adhesion was phenomenally improved. IHC worked very well on these slides. Best regards, Regan Regan Fulton, M.D., Ph.D. CEO and Co-Founder Array Science, LLC 475 Gate 5 Road, #100 Sausalito, CA 94965 (415) 577-7360 www.arrayscience.com On Tue, Mar 1, 2022 at 11:30 AM Hobbs, Carl via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Yes...a big problem for me, too....if you are using HIER?? > Cartilage and bone are major tissue retention problems > I cut Pwax sections of mouse long bones/knee joints for anti GFP IHC > I still get a % fall off but, using manual HIER at 90C I manage to get > enough sections to get good results > However, I imagine that your autoIHC stainer prob uses 90C HIER? > I find Trajan 3 series slides more reliable than Superfrost Plus slides. > Sure, I need to dry my slides for 24 hrs in an operating fume hood ( we > can't turn ours off/down) > Then I place slides in a 60C oven for 2hrs. > Then dewax etc.... > > Be interesting to read of other suggestions > > Best wishes > > Carl > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > > > 020 7848 6810 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From VKurth at uwhealth.org Tue Mar 1 14:19:13 2022 From: VKurth at uwhealth.org (Kurth, Virginia L) Date: Tue, 1 Mar 2022 20:19:13 +0000 Subject: [Histonet] IHC staining of cartilage In-Reply-To: References: Message-ID: Make sure they sit at room temperature longer than normal and then dry them longer. I found that cutting them thinner (3 microns) also helps. Ginny Kurth, HT, (ASCP)CM UW Health Surgical Pathology 1111 Highland Ave Madison, WI -----Original Message----- From: Hobbs, Carl via Histonet Sent: Tuesday, March 01, 2022 1:30 PM To: histonet Subject: Re: [Histonet] IHC staining of cartilage WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Yes...a big problem for me, too....if you are using HIER?? Cartilage and bone are major tissue retention problems I cut Pwax sections of mouse long bones/knee joints for anti GFP IHC I still get a % fall off but, using manual HIER at 90C I manage to get enough sections to get good results However, I imagine that your autoIHC stainer prob uses 90C HIER? I find Trajan 3 series slides more reliable than Superfrost Plus slides. Sure, I need to dry my slides for 24 hrs in an operating fume hood ( we can't turn ours off/down) Then I place slides in a 60C oven for 2hrs. Then dewax etc.... Be interesting to read of other suggestions Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thomas6093 at yahoo.com Wed Mar 2 20:54:57 2022 From: thomas6093 at yahoo.com (Thomas Huynh) Date: Thu, 3 Mar 2022 02:54:57 +0000 (UTC) Subject: [Histonet] Equipment donation References: <522278014.307353.1646276097171.ref@mail.yahoo.com> Message-ID: <522278014.307353.1646276097171@mail.yahoo.com> Good evening, I am posting for a friend who is a professor at the local university. They are teaching basic research protocols and in need of basic histology equipment such as: tissue processor, embedding unit and microtome. They would like to ask anyone who has aging equipment but still function and willing to donate to the school. Thank you in advance Thomas713-297-1606? From relia1 at earthlink.net Thu Mar 3 08:30:13 2022 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 3 Mar 2022 09:30:13 -0500 Subject: [Histonet] Histotechnology Professionals Day is Thursday March 10th. That's just a week away! Here are some great resources for you and your team. Message-ID: <059301d82f0b$32dd9dc0$9898d940$@earthlink.net> Hi Histopeeps! March 10th is Histotechnology Professionals Day! I hope you are doing well. Let me be among the first to wish you: Happy Histotechnology Professionals Day!! I Hope Your Facility Is Planning Some Fun, Informative And Special Events to Celebrate Because You and Your histopeeps deserve It! Many labs are celebrating with trivia contests, luncheons, themed days and general activities to promote their profession. Check out the NSH's website section for Histotechnology Professionals Day Here is the link: https://www.nsh.org/nsh-events/histotechnology-day Don't Miss the Virtual Happy Hours. They are scheduled in the evening and in the morning and are featuring fun games, socializing and a signature drink for the evening and a signature smoothie for the morning! Hope to see you there!! Also I would love to hear what you are doing to celebrate so please shoot me back an e-mail and let me know. If you need help with anything please contact me! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions From tissuearray at hotmail.com Fri Mar 4 06:57:22 2022 From: tissuearray at hotmail.com (Thom) Date: Fri, 4 Mar 2022 12:57:22 +0000 Subject: [Histonet] Florida travel Jobs and how to get in Message-ID: I am pondering on doing some travel histology. How hard is it to get a license in Florida? I have been in histology for over 25 years, received my HT (ASCP) in 1998. I have a BA but not in a science field. I have worked in EM, FISH, immunos, routine histology, research, TMA construction and I cut fast and clean. Am I qualified to work in the state of Florida or will they expect me to go back to school and other training? They seem to be in some kind of special club or something. I see a lot of histology job openings in Florida, more than anywhere else in the country. Thanks for your time. Thom Thom Jensen HT(ASCP) / TMA technician From mim9060 at nyp.org Fri Mar 4 09:17:46 2022 From: mim9060 at nyp.org (Maimone-Schoen, Michele) Date: Fri, 4 Mar 2022 15:17:46 +0000 Subject: [Histonet] contents of Histonet digest Message-ID: <7ba7d2d8b6c044ab9a7af79e82fa852b@nyp.org> How are labs addressing the new CAP standard ANP.10041: Quality of Formalin Monitoring? Thank you. Michele Maimone-Schoen, MS Manager, Anatomic Pathology New York-Presbyterian Hospital 525 East 68th Street, Starr 1003 New York, NY 10065 212-746-2633 (Office) 212-746-5007 (Fax) 646-856-1738 (Cell) Be Amazing! ---------------------------------------------------------------------- This electronic message is intended to be for the use only of the named recipient, and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. Thank you. code:d34y From bcooper at chla.usc.edu Fri Mar 4 09:41:37 2022 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Fri, 4 Mar 2022 15:41:37 +0000 Subject: [Histonet] contents of Histonet digest In-Reply-To: <7ba7d2d8b6c044ab9a7af79e82fa852b@nyp.org> References: <7ba7d2d8b6c044ab9a7af79e82fa852b@nyp.org> Message-ID: <93de7834-0eed-410a-b2de-2956e911c500@chla.usc.edu> Our formalin vendor is sending us certificates of analysis with every shipment now. We review these forms to ensure the lot is acceptable and file them in a binder. Thanks, Brian Cooper Histology Supervisor Children's Hospital Los Angeles Sent from my mobile On Mar 4, 2022 7:18 AM, "Maimone-Schoen, Michele via Histonet" wrote: ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. How are labs addressing the new CAP standard ANP.10041: Quality of Formalin Monitoring? Thank you. Michele Maimone-Schoen, MS Manager, Anatomic Pathology New York-Presbyterian Hospital 525 East 68th Street, Starr 1003 New York, NY 10065 212-746-2633 (Office) 212-746-5007 (Fax) 646-856-1738 (Cell) Be Amazing! ---------------------------------------------------------------------- This electronic message is intended to be for the use only of the named recipient, and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. Thank you. code:d34y _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/11bVCNysfrfYF_5_qCdAzdYHragLgW_t_YnBmnRG6Co7scldudfZCXCmU4ClRJOO_XOWxt5DQyih0z_l7OEfoqLHBPLIceNELSPtNOKgkAmy0Ililqyki59QBoIBOEdMf4TqJQZd9_1Q9DGX3kw-rz0lKwpyKoMhW16SuRtBBxlH90AlaVArg6Kyh4X3ZP_p9gXSvh0r7NWsyjN0_gEp9kk_8uykP7aZzuvirQmowNx6WxnF8-g9_d03HPnIunwAQvS3R8OSXec55fNqZ-BTCEL6Kl52DfnV3gSNjnPfySLlHd3iERr6q83IOgjaW2A-z-Bi8ot_IHyTJ0Ni5-TYtlvMLz4l3VUGOyVMPG93jx2UhBubHTdsmkdgSj0LGWWSuVcZqu_-AR3OBrhm6NQ1wzQpJ5YzNXSMsgS4MwBqPRZ8WngIUTcJyIPV85gw4FuDgw6FyBaEFq91GkAtDILNkOA/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From mim9060 at nyp.org Fri Mar 4 09:45:59 2022 From: mim9060 at nyp.org (Maimone-Schoen, Michele) Date: Fri, 4 Mar 2022 15:45:59 +0000 Subject: [Histonet] [EXTERNAL] Re: contents of Histonet digest In-Reply-To: <93de7834-0eed-410a-b2de-2956e911c500@chla.usc.edu> References: <7ba7d2d8b6c044ab9a7af79e82fa852b@nyp.org> <93de7834-0eed-410a-b2de-2956e911c500@chla.usc.edu> Message-ID: <2d29ef3dd93242389a608e8b42281c39@nyp.org> Who is your vendor? Thanks for this information. From: Cooper, Brian Sent: Friday, March 4, 2022 10:42 AM To: Maimone-Schoen, Michele ; histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] contents of Histonet digest Our formalin vendor is sending us certificates of analysis with every shipment now. We review these forms to ensure the lot is acceptable and file them in a binder. Thanks, Brian Cooper Histology Supervisor Children's Hospital Los Angeles Sent from my mobile On Mar 4, 2022 7:18 AM, "Maimone-Schoen, Michele via Histonet" > wrote: ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. How are labs addressing the new CAP standard ANP.10041: Quality of Formalin Monitoring? Thank you. Michele Maimone-Schoen, MS Manager, Anatomic Pathology New York-Presbyterian Hospital 525 East 68th Street, Starr 1003 New York, NY 10065 212-746-2633 (Office) 212-746-5007 (Fax) 646-856-1738 (Cell) Be Amazing! ---------------------------------------------------------------------- This electronic message is intended to be for the use only of the named recipient, and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. Thank you. code:d34y _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/11bVCNysfrfYF_5_qCdAzdYHragLgW_t_YnBmnRG6Co7scldudfZCXCmU4ClRJOO_XOWxt5DQyih0z_l7OEfoqLHBPLIceNELSPtNOKgkAmy0Ililqyki59QBoIBOEdMf4TqJQZd9_1Q9DGX3kw-rz0lKwpyKoMhW16SuRtBBxlH90AlaVArg6Kyh4X3ZP_p9gXSvh0r7NWsyjN0_gEp9kk_8uykP7aZzuvirQmowNx6WxnF8-g9_d03HPnIunwAQvS3R8OSXec55fNqZ-BTCEL6Kl52DfnV3gSNjnPfySLlHd3iERr6q83IOgjaW2A-z-Bi8ot_IHyTJ0Ni5-TYtlvMLz4l3VUGOyVMPG93jx2UhBubHTdsmkdgSj0LGWWSuVcZqu_-AR3OBrhm6NQ1wzQpJ5YzNXSMsgS4MwBqPRZ8WngIUTcJyIPV85gw4FuDgw6FyBaEFq91GkAtDILNkOA/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From jmacdonald at mtsac.edu Fri Mar 4 14:58:18 2022 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Fri, 4 Mar 2022 20:58:18 +0000 Subject: [Histonet] California Society for Histotechnology Annual Symposium Message-ID: https://www.californiahistology.org/events/annual-symposium We are back in person for our annual meeting!! From greg.dobbin at gmail.com Mon Mar 7 10:16:33 2022 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Mon, 7 Mar 2022 12:16:33 -0400 Subject: [Histonet] Fixation time and ISH Message-ID: Background: I read on a vendor website that tissues stained using In situ hybridization should not exceed 32 hours fixation. Greater than 32 hours can produce false negative results because the validated retrieving protocol can be inadequate. We in fact have seen evidence of this in our lab (mystifying negative kappa/lambda staining) and we are beginning to realize that over-fixation May we?ll be the issue for us (eg weekend processing). I am proposing that we do what was common practice many years ago. That is, transfer the properly fixed and decalcified bone marrow cores into 70% ETOH until they can be processed into paraffin. My questions: Is anyone else doing this?? Is 70% ETOH still a viable option for labs in this situation?? And? Is there another idea and/or more information out there that could help us in this regard?? Thank you, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From Taylor.Rosa at crl.com Mon Mar 7 13:28:40 2022 From: Taylor.Rosa at crl.com (Rosa, Taylor) Date: Mon, 7 Mar 2022 19:28:40 +0000 Subject: [Histonet] Fixation time and ISH In-Reply-To: References: Message-ID: Hi Greg, For any that have ISH staining, we add to the protocol that fixation should be 24-32h. Tissues are transferred to 70% EtOH after that. One thing to note is that for brain tissue, it should not be in 70% for longer than 24 hours, because tissue artifacts (vacuolization) will occur. Thanks, Taylor Rosa -----Original Message----- From: Greg Dobbin Sent: 07-Mar-2022 11:17 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fixation time and ISH Background: I read on a vendor website that tissues stained using In situ hybridization should not exceed 32 hours fixation. Greater than 32 hours can produce false negative results because the validated retrieving protocol can be inadequate. We in fact have seen evidence of this in our lab (mystifying negative kappa/lambda staining) and we are beginning to realize that over-fixation May we?ll be the issue for us (eg weekend processing). I am proposing that we do what was common practice many years ago. That is, transfer the properly fixed and decalcified bone marrow cores into 70% ETOH until they can be processed into paraffin. My questions: Is anyone else doing this?? Is 70% ETOH still a viable option for labs in this situation?? And? Is there another idea and/or more information out there that could help us in this regard?? Thank you, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From Charles.Bacon at baystatehealth.org Tue Mar 8 07:11:50 2022 From: Charles.Bacon at baystatehealth.org (Bacon, Charles) Date: Tue, 8 Mar 2022 13:11:50 +0000 Subject: [Histonet] IHC staining of cartilage Message-ID: We use P8920 Sigma-Aldrich Poly-L-lysine solution. Great retention without excess background staining. You can prep a rack of slides in diluted solution but we put a gloved finger over the end of the bottle and and swipe it down the slide at full strength. Let the slide dry and move forward as you normally would. Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786? Fax: 413-322-4790 Charles.Bacon at baystatehealth.org -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Wednesday, March 2, 2022 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 220, Issue 2 ? EXTERNAL EMAIL Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!DNKm7xijBw!QHRtbD7GLZW7UOIDd0PZve5c_AlDixDOj3pnc7epMKm8jxv4F9_wxmfqmGVNeJGbQ9lUKw$ or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." EXTERNAL EMAIL: This email came from outside of Baystate. 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From Nancy.Schmitt at mercyhealth.com Tue Mar 8 11:54:38 2022 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Tue, 8 Mar 2022 17:54:38 +0000 Subject: [Histonet] formalin in OR Message-ID: Hello- I would appreciate input on how you are getting formalin on to the specimens: * Is it done in OR * Are specimens brough to pathology and add formalin there * If so - does lab or OR add the formalin * Other? Are you Joint Commission? Thank you! Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Dubuque, IA Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From chenoa at pathologywatch.com Tue Mar 8 12:17:13 2022 From: chenoa at pathologywatch.com (Chenoa Hardwick) Date: Tue, 8 Mar 2022 12:17:13 -0600 Subject: [Histonet] Post message to Histonet In-Reply-To: References: Message-ID: Please post to histonet: Job opening for histotechs (all shifts) and Pathology laboratory manager position (Utah) Contact Chenoa at pathologywatch.com for inquiries. Thanks *Chenoa Hardwick* VP of Laboratory Services email: chenoa at pathologywatch.com mobile: +1 972.351.0774 On Tue, Mar 8, 2022 at 12:08 PM Chenoa Hardwick wrote: > I would like to post a job opening on Histonet. > > Please see attached job description. > > Thanks > > *Chenoa Hardwick* > > VP of Laboratory Services > > email: chenoa at pathologywatch.com > > mobile: +1 972.351.0774 > > > > ---- Secured by Paubox - HITRUST CSF certified https://www.paubox.com ---- From greg.dobbin at gmail.com Tue Mar 8 14:00:14 2022 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Tue, 8 Mar 2022 16:00:14 -0400 Subject: [Histonet] formalin in OR Message-ID: Hi Nancy, All routine specimens in our hospital are placed in formalin in the OR. Breast lumps and mastectomy specimens are sent up fresh so that they arrive STAT (to minimize cold ischemic times) and lymph nodes for lymphoma protocol are also sent up fresh. [*they actually send sentinel nodes up fresh too- it was just less confusing*] Diagnostic Imaging will place routine needle core biopsies directly in formalin (e.g needle cores of breast, prostate, non-lympoma lymph nodes and other misc. tissue masses). Lymph node cores for lymphoma protocol and renal cores are sent to the lab fresh, in a container on saline soaked Telfa pads. Endoscopy places all of their specimens directly in formalin. The derm clinic will place all routine skins directly into formalin but specimens destined for immunofluoresence are sent up to the lab fresh in a container on saline soaked Telfa pads. EBUS specimens are split between Cytology and Histology...the histo specimens go directly into formalin. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From chenoa at pathologywatch.com Tue Mar 8 14:19:11 2022 From: chenoa at pathologywatch.com (Chenoa Hardwick) Date: Tue, 8 Mar 2022 14:19:11 -0600 Subject: [Histonet] posting Message-ID: Please post: Job open - Salt Lake City, UT - Pathology Lab Manager Relocation, sign on bonus available. Please contact Chenoa Hardwick at chenoa at pathologywatch.com 972-351-0774 *Chenoa Hardwick* VP of Laboratory Services email: chenoa at pathologywatch.com mobile: +1 972.351.0774 ---- Secured by Paubox - HITRUST CSF certified https://www.paubox.com ---- From john.garratt at ciqc.ca Tue Mar 8 19:28:25 2022 From: john.garratt at ciqc.ca (John Garratt) Date: Wed, 09 Mar 2022 01:28:25 +0000 Subject: [Histonet] formalin in OR In-Reply-To: References: Message-ID: I would not normally promote a product but I have seen the TissueSafe and SealSafe (Milestone) in use at a couple of hospitals for formalin management in the OR and in the lab and was most impressed. John Sent from ProtonMail for iOS On Tue, Mar 8, 2022 at 9:54 AM, Nancy Schmitt via Histonet wrote: > Hello- > I would appreciate input on how you are getting formalin on to the specimens: > > * Is it done in OR > * Are specimens brough to pathology and add formalin there > * If so - does lab or OR add the formalin > * Other? > Are you Joint Commission? > Thank you! > Nancy Schmitt MLT, HT(ASCP) > Pathology Support Services > Dubuque, IA > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From psjayalakshmy at gmail.com Wed Mar 9 20:26:50 2022 From: psjayalakshmy at gmail.com (jayalakshmy p.s) Date: Thu, 10 Mar 2022 07:56:50 +0530 Subject: [Histonet] Histonet Digest, Vol 220, Issue 8 In-Reply-To: References: Message-ID: Hello all, I would like to know whether Immunohistochemistry markers can be used after its expiry date(atleast in resource poor countries). If yes, for how much period of time and what is the criteria to look for? Thanks and regards Jayalakshmy On Wed, Mar 9, 2022, 11:30 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Re: Post message to Histonet (Chenoa Hardwick) > 2. Re: formalin in OR (Greg Dobbin) > 3. posting (Chenoa Hardwick) > 4. Re: formalin in OR (John Garratt) > > > > ---------- Forwarded message ---------- > From: Chenoa Hardwick > To: histonet at lists.utsouthwestern.edu > Cc: > Bcc: > Date: Tue, 8 Mar 2022 12:17:13 -0600 > Subject: Re: [Histonet] Post message to Histonet > Please post to histonet: > > Job opening for histotechs (all shifts) and Pathology laboratory manager > position (Utah) > > Contact Chenoa at pathologywatch.com for inquiries. > > Thanks > > *Chenoa Hardwick* > > VP of Laboratory Services > > email: chenoa at pathologywatch.com > > mobile: +1 972.351.0774 > > > > > > On Tue, Mar 8, 2022 at 12:08 PM Chenoa Hardwick > > wrote: > > > I would like to post a job opening on Histonet. > > > > Please see attached job description. > > > > Thanks > > > > *Chenoa Hardwick* > > > > VP of Laboratory Services > > > > email: chenoa at pathologywatch.com > > > > mobile: +1 972.351.0774 > > > > > > > > > > > ---- > Secured by Paubox - HITRUST CSF certified > https://www.paubox.com > ---- > > > > ---------- Forwarded message ---------- > From: Greg Dobbin > To: Nancy.Schmitt at mercyhealth.com, histonet at lists.utsouthwestern.edu > Cc: > Bcc: > Date: Tue, 8 Mar 2022 16:00:14 -0400 > Subject: Re: [Histonet] formalin in OR > Hi Nancy, > All routine specimens in our hospital are placed in formalin in the OR. > Breast lumps and mastectomy specimens are sent up fresh so that they arrive > STAT (to minimize cold ischemic times) and lymph nodes for lymphoma > protocol are also sent up fresh. [*they actually send sentinel nodes up > fresh too- it was just less confusing*] > > Diagnostic Imaging will place routine needle core biopsies directly in > formalin (e.g needle cores of breast, prostate, non-lympoma lymph nodes and > other misc. tissue masses). Lymph node cores for lymphoma protocol and > renal cores are sent to the lab fresh, in a container on saline soaked > Telfa pads. > > Endoscopy places all of their specimens directly in formalin. The derm > clinic will place all routine skins directly into formalin but specimens > destined for immunofluoresence are sent up to the lab fresh in a container > on saline soaked Telfa pads. EBUS specimens are split between Cytology and > Histology...the histo specimens go directly into formalin. > > Greg > > -- > *Greg Dobbin* > 1205 Pleasant Grove Rd > RR#2 York, > PE C0A 1P0 > > > *Everything in moderation...even moderation itself**!* > > > > > ---------- Forwarded message ---------- > From: Chenoa Hardwick > To: histonet at lists.utsouthwestern.edu > Cc: > Bcc: > Date: Tue, 8 Mar 2022 14:19:11 -0600 > Subject: [Histonet] posting > Please post: > > Job open - Salt Lake City, UT - Pathology Lab Manager > Relocation, sign on bonus available. > > Please contact Chenoa Hardwick at chenoa at pathologywatch.com 972-351-0774 > > *Chenoa Hardwick* > > VP of Laboratory Services > > email: chenoa at pathologywatch.com > > mobile: +1 972.351.0774 > > > > > > ---- > Secured by Paubox - HITRUST CSF certified > https://www.paubox.com > ---- > > > > ---------- Forwarded message ---------- > From: John Garratt > To: Nancy Schmitt , " > histonet at lists.utsouthwestern.edu" > Cc: > Bcc: > Date: Wed, 09 Mar 2022 01:28:25 +0000 > Subject: Re: [Histonet] formalin in OR > I would not normally promote a product but I have seen the TissueSafe and > SealSafe (Milestone) in use at a couple of hospitals for formalin > management in the OR and in the lab and was most impressed. > > John > > Sent from ProtonMail for iOS > > On Tue, Mar 8, 2022 at 9:54 AM, Nancy Schmitt via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hello- > > I would appreciate input on how you are getting formalin on to the > specimens: > > > > * Is it done in OR > > * Are specimens brough to pathology and add formalin there > > * If so - does lab or OR add the formalin > > * Other? > > Are you Joint Commission? > > Thank you! > > Nancy Schmitt MLT, HT(ASCP) > > Pathology Support Services > > Dubuque, IA > > > > Confidentiality Notice: > > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jeffrey.Rinker at SanfordHealth.org Thu Mar 10 09:21:39 2022 From: Jeffrey.Rinker at SanfordHealth.org (Rinker,Jeffrey) Date: Thu, 10 Mar 2022 15:21:39 +0000 Subject: [Histonet] best paraffin Message-ID: I was wondering what people think is the best paraffin to use. I find it hard to get a good read on what is best for our lab. We do around 60-100 blocks a day and do a bx and surgical run overnight. I think that we are having problems with cutting because we are not optimized and i am trying to fix that. ----------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain privileged and confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From john.garratt at ciqc.ca Thu Mar 10 11:01:03 2022 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 10 Mar 2022 17:01:03 +0000 Subject: [Histonet] best paraffin In-Reply-To: References: Message-ID: The paraffins available from lab suppliers (not candle makers) is all of excellent quality these days. There is some variability, but choice of paraffin usually comes down to personal preference. If you are having problems cutting it may well be related to other factors like fixation and processing, and of course, the microtomy. What are the issues you are encountering? John www.cpqa.ca Sent from ProtonMail for iOS On Thu, Mar 10, 2022 at 7:21 AM, Rinker,Jeffrey via Histonet wrote: > I was wondering what people think is the best paraffin to use. I find it hard to get a good read on what is best for our lab. We do around 60-100 blocks a day and do a bx and surgical run overnight. I think that we are having problems with cutting because we are not optimized and i am trying to fix that. > ----------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > privileged and confidential information. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender by reply e-mail and destroy > all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren at gmail.com Thu Mar 10 11:22:20 2022 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 10 Mar 2022 11:22:20 -0600 Subject: [Histonet] best paraffin Message-ID: Paraplast plus is my personal favorite. On March 10, 2022, at 11:04 AM, John Garratt via Histonet wrote: The paraffins available from lab suppliers (not candle makers) is all of excellent quality these days. There is some variability, but choice of paraffin usually comes down to personal preference. If you are having problems cutting it may well be related to other factors like fixation and processing, and of course, the microtomy. What are the issues you are encountering? John www.cpqa.ca Sent from ProtonMail for iOS On Thu, Mar 10, 2022 at 7:21 AM, Rinker,Jeffrey via Histonet wrote: > I was wondering what people think is the best paraffin to use. I find it hard to get a good read on what is best for our lab. We do around 60-100 blocks a day and do a bx and surgical run overnight. I think that we are having problems with cutting because we are not optimized and i am trying to fix that. > ----------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > privileged and confidential information. Any unauthorized review, use, > disclosure or distribution is prohibited. If you are not the intended > recipient, please contact the sender by reply e-mail and destroy > all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper at chla.usc.edu Thu Mar 10 11:40:45 2022 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Thu, 10 Mar 2022 17:40:45 +0000 Subject: [Histonet] best paraffin In-Reply-To: References: Message-ID: <7af955ab47f14948800a783aa7b30275@chla.usc.edu> Paraplast for processing and Paraplast Xtra for microtomy at our institution. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper at chla.usc.edu -----Original Message----- From: Jay Lundgren via Histonet Sent: Thursday, March 10, 2022 9:22 AM To: John Garratt ; Rinker, Jeffrey ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] best paraffin (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. Paraplast plus is my personal favorite. On March 10, 2022, at 11:04 AM, John Garratt via Histonet wrote: The paraffins available from lab suppliers (not candle makers) is all of excellent quality these days. There is some variability, but choice of paraffin usually comes down to personal preference. If you are having problems cutting it may well be related to other factors like fixation and processing, and of course, the microtomy. What are the issues you are encountering? John http://secure-web.cisco.com/1071-nF34Jc0Huepy-CD2D7i4jVe0u3CQxXT-sfZxfJTPdesE2Cm8FUb5nKm9zlp5LAeGb2S-mRT5t0YNkJXRP9yxFC6eIbZWkvU2xGhG7zo9bs6HRYe3J3_UeSdo0VQTrph5P75aQOH1s58spSuO-R3u4LJK8DTNJuJq_j_OtDg2dWHytU5gKPuhKt-OY9w6NdjLPdy_fwi19pr6qF3YFMgPjoLMkuKN_5Xx2Eql8JtaJYuRKcyEDRScQsFXnwW9me2ojfswJ7R5PEGkbd-xjFKSxPuf8S9BimLtV6LbCgcnEhGxaKhr_PQX-UQjP4NUeHoJBE-ATPlQe-a5Im_F4mZt092VRWpnwASWIXe8ob5w6_695blw4gK0jAPU7PyEGtfhIhftxwnB6u-Bfc818ugk8ZBulD3LVuGYoXwjUo2B3s-1MnYxRvVbWseO_ulPMS29Byi2oEe5SRg8lHMc_Q/http%3A%2F%2Fwww.cpqa.ca Sent from ProtonMail for iOS On Thu, Mar 10, 2022 at 7:21 AM, Rinker,Jeffrey via Histonet wrote: > I was wondering what people think is the best paraffin to use. I find it hard to get a good read on what is best for our lab. We do around 60-100 blocks a day and do a bx and surgical run overnight. I think that we are having problems with cutting because we are not optimized and i am trying to fix that. > ---------------------------------------------------------------------- > - Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the intended recipient(s) and may > contain privileged and confidential information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://secure-web.cisco.com/1TKwpIkxGaOgvuz-4YCLvI6bDNgo5cwRKuxkZF_PPi > kd-8hxYiAzUd8CUPp6I5P9I1YiYCN1dFXYd0U7oY9b9LvQLn5S2CB6LbUUAsjXPqVmRR1L > 8d5XUahFQP8UfJFpIfqxeDRDdK6eJ-TsB6EDVR_r8yH8VIgC2D9VJbsxJCIN4_pikz4BhD > 2s49yLzHW8dy3SF9KteCp6ngy_q1ZkwU6KUsxFbMoFsABzVbBML3pnjbTMdFiPJchkFef1 > kh-fZtDii5YZa3jKDw5Phdxe6pnRaNm6kfONnkIB-kUeku6X_Vc6ygBFPKNI4UQD4mHdxY > RVhOQA6X4qIkM38yr4Q8b24xl9PAuSsVqD1IVadnSN58evCKM6-A7wmNm3oRTjMRHOgnaz > mRP9X0Q4mFbsvIgapbPiiLXV_DXuQHyL1cYOb2hT4uLf66Ckev4IKsSDsWL2Fu3h94c0Fs > aFZWFIa1g/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2 > Fhistonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1TKwpIkxGaOgvuz-4YCLvI6bDNgo5cwRKuxkZF_PPikd-8hxYiAzUd8CUPp6I5P9I1YiYCN1dFXYd0U7oY9b9LvQLn5S2CB6LbUUAsjXPqVmRR1L8d5XUahFQP8UfJFpIfqxeDRDdK6eJ-TsB6EDVR_r8yH8VIgC2D9VJbsxJCIN4_pikz4BhD2s49yLzHW8dy3SF9KteCp6ngy_q1ZkwU6KUsxFbMoFsABzVbBML3pnjbTMdFiPJchkFef1kh-fZtDii5YZa3jKDw5Phdxe6pnRaNm6kfONnkIB-kUeku6X_Vc6ygBFPKNI4UQD4mHdxYRVhOQA6X4qIkM38yr4Q8b24xl9PAuSsVqD1IVadnSN58evCKM6-A7wmNm3oRTjMRHOgnazmRP9X0Q4mFbsvIgapbPiiLXV_DXuQHyL1cYOb2hT4uLf66Ckev4IKsSDsWL2Fu3h94c0FsaFZWFIa1g/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1TKwpIkxGaOgvuz-4YCLvI6bDNgo5cwRKuxkZF_PPikd-8hxYiAzUd8CUPp6I5P9I1YiYCN1dFXYd0U7oY9b9LvQLn5S2CB6LbUUAsjXPqVmRR1L8d5XUahFQP8UfJFpIfqxeDRDdK6eJ-TsB6EDVR_r8yH8VIgC2D9VJbsxJCIN4_pikz4BhD2s49yLzHW8dy3SF9KteCp6ngy_q1ZkwU6KUsxFbMoFsABzVbBML3pnjbTMdFiPJchkFef1kh-fZtDii5YZa3jKDw5Phdxe6pnRaNm6kfONnkIB-kUeku6X_Vc6ygBFPKNI4UQD4mHdxYRVhOQA6X4qIkM38yr4Q8b24xl9PAuSsVqD1IVadnSN58evCKM6-A7wmNm3oRTjMRHOgnazmRP9X0Q4mFbsvIgapbPiiLXV_DXuQHyL1cYOb2hT4uLf66Ckev4IKsSDsWL2Fu3h94c0FsaFZWFIa1g/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From john.garratt at ciqc.ca Thu Mar 10 11:51:01 2022 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 10 Mar 2022 17:51:01 +0000 Subject: [Histonet] best paraffin In-Reply-To: <7af955ab47f14948800a783aa7b30275@chla.usc.edu> References: <7af955ab47f14948800a783aa7b30275@chla.usc.edu> Message-ID: Interesting that you are mixing the two. Is that common practice out there? John Sent from ProtonMail for iOS On Thu, Mar 10, 2022 at 9:40 AM, Cooper, Brian wrote: > Paraplast for processing and Paraplast Xtra for microtomy at our institution. > > Thanks, > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > bcooper at chla.usc.edu > > -----Original Message----- > From: Jay Lundgren via Histonet > Sent: Thursday, March 10, 2022 9:22 AM > To: John Garratt ; Rinker, Jeffrey ; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] best paraffin (EXTERNAL EMAIL) > > ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. > > Paraplast plus is my personal favorite. > > On March 10, 2022, at 11:04 AM, John Garratt via Histonet wrote: > > The paraffins available from lab suppliers (not candle makers) is all of excellent quality these days. There is some variability, but choice of paraffin usually comes down to personal preference. > If you are having problems cutting it may well be related to other factors like fixation and processing, and of course, the microtomy. > What are the issues you are encountering? > > John > http://secure-web.cisco.com/1071-nF34Jc0Huepy-CD2D7i4jVe0u3CQxXT-sfZxfJTPdesE2Cm8FUb5nKm9zlp5LAeGb2S-mRT5t0YNkJXRP9yxFC6eIbZWkvU2xGhG7zo9bs6HRYe3J3_UeSdo0VQTrph5P75aQOH1s58spSuO-R3u4LJK8DTNJuJq_j_OtDg2dWHytU5gKPuhKt-OY9w6NdjLPdy_fwi19pr6qF3YFMgPjoLMkuKN_5Xx2Eql8JtaJYuRKcyEDRScQsFXnwW9me2ojfswJ7R5PEGkbd-xjFKSxPuf8S9BimLtV6LbCgcnEhGxaKhr_PQX-UQjP4NUeHoJBE-ATPlQe-a5Im_F4mZt092VRWpnwASWIXe8ob5w6_695blw4gK0jAPU7PyEGtfhIhftxwnB6u-Bfc818ugk8ZBulD3LVuGYoXwjUo2B3s-1MnYxRvVbWseO_ulPMS29Byi2oEe5SRg8lHMc_Q/http%3A%2F%2Fwww.cpqa.ca > > Sent from ProtonMail for iOS > > On Thu, Mar 10, 2022 at 7:21 AM, Rinker,Jeffrey via Histonet wrote: > >> I was wondering what people think is the best paraffin to use. I find it hard to get a good read on what is best for our lab. We do around 60-100 blocks a day and do a bx and surgical run overnight. I think that we are having problems with cutting because we are not optimized and i am trying to fix that. >> ---------------------------------------------------------------------- >> - Confidentiality Notice: This e-mail message, including any >> attachments, is for the sole use of the intended recipient(s) and may >> contain privileged and confidential information. Any unauthorized >> review, use, disclosure or distribution is prohibited. If you are not >> the intended recipient, please contact the sender by reply e-mail and >> destroy all copies of the original message. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://secure-web.cisco.com/1TKwpIkxGaOgvuz-4YCLvI6bDNgo5cwRKuxkZF_PPi >> kd-8hxYiAzUd8CUPp6I5P9I1YiYCN1dFXYd0U7oY9b9LvQLn5S2CB6LbUUAsjXPqVmRR1L >> 8d5XUahFQP8UfJFpIfqxeDRDdK6eJ-TsB6EDVR_r8yH8VIgC2D9VJbsxJCIN4_pikz4BhD >> 2s49yLzHW8dy3SF9KteCp6ngy_q1ZkwU6KUsxFbMoFsABzVbBML3pnjbTMdFiPJchkFef1 >> kh-fZtDii5YZa3jKDw5Phdxe6pnRaNm6kfONnkIB-kUeku6X_Vc6ygBFPKNI4UQD4mHdxY >> RVhOQA6X4qIkM38yr4Q8b24xl9PAuSsVqD1IVadnSN58evCKM6-A7wmNm3oRTjMRHOgnaz >> mRP9X0Q4mFbsvIgapbPiiLXV_DXuQHyL1cYOb2hT4uLf66Ckev4IKsSDsWL2Fu3h94c0Fs >> aFZWFIa1g/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2 >> Fhistonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://secure-web.cisco.com/1TKwpIkxGaOgvuz-4YCLvI6bDNgo5cwRKuxkZF_PPikd-8hxYiAzUd8CUPp6I5P9I1YiYCN1dFXYd0U7oY9b9LvQLn5S2CB6LbUUAsjXPqVmRR1L8d5XUahFQP8UfJFpIfqxeDRDdK6eJ-TsB6EDVR_r8yH8VIgC2D9VJbsxJCIN4_pikz4BhD2s49yLzHW8dy3SF9KteCp6ngy_q1ZkwU6KUsxFbMoFsABzVbBML3pnjbTMdFiPJchkFef1kh-fZtDii5YZa3jKDw5Phdxe6pnRaNm6kfONnkIB-kUeku6X_Vc6ygBFPKNI4UQD4mHdxYRVhOQA6X4qIkM38yr4Q8b24xl9PAuSsVqD1IVadnSN58evCKM6-A7wmNm3oRTjMRHOgnazmRP9X0Q4mFbsvIgapbPiiLXV_DXuQHyL1cYOb2hT4uLf66Ckev4IKsSDsWL2Fu3h94c0FsaFZWFIa1g/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://secure-web.cisco.com/1TKwpIkxGaOgvuz-4YCLvI6bDNgo5cwRKuxkZF_PPikd-8hxYiAzUd8CUPp6I5P9I1YiYCN1dFXYd0U7oY9b9LvQLn5S2CB6LbUUAsjXPqVmRR1L8d5XUahFQP8UfJFpIfqxeDRDdK6eJ-TsB6EDVR_r8yH8VIgC2D9VJbsxJCIN4_pikz4BhD2s49yLzHW8dy3SF9KteCp6ngy_q1ZkwU6KUsxFbMoFsABzVbBML3pnjbTMdFiPJchkFef1kh-fZtDii5YZa3jKDw5Phdxe6pnRaNm6kfONnkIB-kUeku6X_Vc6ygBFPKNI4UQD4mHdxYRVhOQA6X4qIkM38yr4Q8b24xl9PAuSsVqD1IVadnSN58evCKM6-A7wmNm3oRTjMRHOgnazmRP9X0Q4mFbsvIgapbPiiLXV_DXuQHyL1cYOb2hT4uLf66Ckev4IKsSDsWL2Fu3h94c0FsaFZWFIa1g/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From Charles.Bacon at baystatehealth.org Thu Mar 10 12:22:13 2022 From: Charles.Bacon at baystatehealth.org (Bacon, Charles) Date: Thu, 10 Mar 2022 18:22:13 +0000 Subject: [Histonet] best paraffin Message-ID: Over all of my testing I found there are several factors to consider when choosing paraffin: 1. Processor type 2. Tissue type 3. Use - embedding vs. processing The processor type my dictate limitations on paraffin selection. For example we were told not to use Paraplast Plus in a processor that has a stainless steel retorts as the DSMO can ruin the surface over time. The tissue type may dictate as infiltration is different through biopsies vs. fatty breast cases. Most do well with both but you may want to factor this in during your search. The use is the biggest deal breaker when it comes to paraffin. If there is too much plastic vs. wax it will not infiltrate during processing. If there is not enough plastic it won't section well. Over the years I have had good luck with several products. I loved Paraplast Plus but when we upgraded our processor we had to switch because of the stainless steel retort. I will say, we were in search of a combination paraffin that we could use for both embedding and processing. We settled on the product from Polyscientific: Infiltrating/Embedding Paraffin Prills (Item Number: c827) We love it. Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786? Fax: 413-322-4790 Charles.Bacon at baystatehealth.org -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Thursday, March 10, 2022 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 220, Issue 9 ? EXTERNAL EMAIL Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!DNKm7xijBw!R-pIa9J6LbBwtKTRjjzM6FSZItqXod0NIyxzJCrVcydrRckGbc6IWcoWSZ_LicKdlkL7ew$ or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." EXTERNAL EMAIL: This email came from outside of Baystate. 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From alida.bailleul at gmail.com Fri Mar 11 00:50:34 2022 From: alida.bailleul at gmail.com (Alida Bailleul) Date: Fri, 11 Mar 2022 14:50:34 +0800 Subject: [Histonet] Vitality versus viability In-Reply-To: References: Message-ID: Dear Histonet, What is the difference between cell vitality or cell viability? Is there a difference between a viable cell and a vital cell? Than you very much for your help All the best Alida Bailleul -- Dr. Alida M. Bailleul Associate Professor Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences www.ivpp-avianevolution.com & Research Associate of Paleontology, Museum of the Rockies, Montana State University Google Scholar - ResearchGate From Reuel.Cornelia at tsrh.org Fri Mar 11 08:36:02 2022 From: Reuel.Cornelia at tsrh.org (Reuel Cornelia) Date: Fri, 11 Mar 2022 14:36:02 +0000 Subject: [Histonet] Multiplex IHC Message-ID: Anyone doing multiplex IHC. I have not heard of it until today. Any reference or recommendations with regards to instrumentation and protocol would be highly appreciated. Thank you. Reuel Cornelia Cellular Pathology Laboratory Supervisor Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel. Number: 214-559-7766 FAX Number: 214-347-4806 Scottish Rite for Children is a world-renowned leader in the treatment of orthopedic conditions such as scoliosis, clubfoot, hand differences, hip disorders, sports injuries and fractures, as well as certain related arthritic and neurological disorders and learning disorders, such as dyslexia. Patients receive treatment regardless of the family's ability to pay. For more information about services available at our Dallas or Frisco campuses, visit scottishriteforchildren.org. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. From jackdodo1959 at gmail.com Fri Mar 11 08:52:18 2022 From: jackdodo1959 at gmail.com (Jack Dodo) Date: Fri, 11 Mar 2022 14:52:18 +0000 Subject: [Histonet] Remove email Message-ID: Please remove my email. Thanks Sent from my Verizon, Samsung Galaxy smartphone Get Outlook for Android From jhill at vet.k-state.edu Wed Mar 16 11:07:29 2022 From: jhill at vet.k-state.edu (Jennifer Phinney) Date: Wed, 16 Mar 2022 16:07:29 +0000 Subject: [Histonet] Sakura SmartWrite software issue Message-ID: Hello Histonetters, For those using the Sakura SmartWrite cassette printer, how many of you are using the SmartWrite Software V 3.1.0 that came out in 2019? We don't have the autoloader, so we print one cassette at a time. We recently updated from the 2017 version, and have ran into an issue with the case information not clearing out after printing. We have to manually delete our accession number and reset the number of cassettes we are printing each time. I have worked with Sakura to try and figure out why the information is not clearing out as it's supposed to. We have rebuilt our templates and have not been able to correct the issue. Sakura is telling us no one else has had this issue with the current version of the software. Which is not the first time a company has tried to gaslight us by telling us we are the only customer experiencing a widespread issue. We are going to reinstall the older software version (with it's own bugs/issues) in the meantime, since it's less problematic. Has anyone else experienced this issue? Were you able to correct the problem? Thanks, Jennifer Phinney M.S. QIHC Histology Laboratory Administrator KSVDL From tony.henwood at health.nsw.gov.au Wed Mar 16 20:18:09 2022 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 17 Mar 2022 01:18:09 +0000 Subject: [Histonet] Histonet Digest, Vol 220, Issue 8 In-Reply-To: References: Message-ID: Hi Jayalakshmy, This our policy at the Kid's hospital in Sydney: Validation of Expired Antibodies Usually when a new concentrated antibody is received it will have an expiry date of around 2 years from receipt but usually we can continue to use antibodies well past this expiry date. If the antibody continues to stain control sections appropriately, with no loss of sensitivity and no increase in non-specific staining then its use should be continued. If positive control samples are deemed unsatisfactory, even if the antibody is within the manufacturer?s printed expiration date, evaluation of the clinical specimen is aborted and the test deemed invalid. The quality of the primary antibody is therefore not based on an expiration date, but rather on its performance on a case-by-case basis with appropriate positive and negative control samples (1). Several authors have investigated whether the shelf-life of diagnostic antibodies was longer than the expiry date on the label. They found them to work perfectly on routine histology sections (1-4). Monoclonal antibodies originally supplied as culture supernatants or as ascites (neat or diluted), of all isotypes, as well as all of the polyclonal antibodies, produced satisfactory staining irrespective of their age. Notable exceptions were ammonium-precipitated, IgM or conjugated antibodies. The policy at CHW is, when an antibody has reached past its expiry date, its control is tested to ensure that there has been no loss of sensitivity in the test. This is now controlled through iPassport, where a task is attached to the antibody requesting validation of control when the antibody is expired. This can be easily done by using the History Screen and looking for use of this antibody within the last two weeks. If results of control are acceptable, another task is instigated for 6 months hence. For antibody concentrates that are received without an expiry date, a verification is scheduled 12 months after receipt of the antibody. If an antibody fails to perform to expectations than a Corrective Action Request is instigated in iPassport and appropriate investigation is instituted. References: 1. Savage, E. C., & DeYoung, B. R. (2010). Antibody Expiration in the Context of Resource Limitation What Is the Evidence Basis?. American journal of clinical pathology, 134(1), 60-64. 2. Balaton, A. J., Drachenberg, C. B., Rucker, C., Vaury, P., & Papadimitriou, J. C. (1999). Satisfactory performance of primary antibodies beyond manufacturers' recommended expiration dates. Applied Immunohistochemistry & Molecular Morphology, 7(3), 221. 3. Argentieri, M. C., Pilla, D., Vanzati, A., Lonardi, S., Facchetti, F., Doglioni, C., & Cattoretti, G. (2013). Antibodies are forever: a study using 12-26?year?old expired antibodies. Histopathology, 63(6), 869-876. 4. Drachenberg, C. B., Papadimitriou, J. C., Balaton, A. J., & Vaury, P. (2001). The total test approach to standardization of immunohistochemistry. Archives of pathology & laboratory medicine, 125(4), 471-471. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: jayalakshmy p.s via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 10 March 2022 1:27 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 220, Issue 8 Hello all, I would like to know whether Immunohistochemistry markers can be used after its expiry date(atleast in resource poor countries). If yes, for how much period of time and what is the criteria to look for? Thanks and regards Jayalakshmy On Wed, Mar 9, 2022, 11:30 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Re: Post message to Histonet (Chenoa Hardwick) > 2. Re: formalin in OR (Greg Dobbin) > 3. posting (Chenoa Hardwick) > 4. Re: formalin in OR (John Garratt) > > > > ---------- Forwarded message ---------- > From: Chenoa Hardwick > To: histonet at lists.utsouthwestern.edu > Cc: > Bcc: > Date: Tue, 8 Mar 2022 12:17:13 -0600 > Subject: Re: [Histonet] Post message to Histonet Please post to > histonet: > > Job opening for histotechs (all shifts) and Pathology laboratory > manager position (Utah) > > Contact Chenoa at pathologywatch.com for inquiries. > > Thanks > > *Chenoa Hardwick* > > VP of Laboratory Services > > email: chenoa at pathologywatch.com > > mobile: +1 972.351.0774 > > > > > > On Tue, Mar 8, 2022 at 12:08 PM Chenoa Hardwick > > > wrote: > > > I would like to post a job opening on Histonet. > > > > Please see attached job description. > > > > Thanks > > > > *Chenoa Hardwick* > > > > VP of Laboratory Services > > > > email: chenoa at pathologywatch.com > > > > mobile: +1 972.351.0774 > > > > > > > > > > > ---- > Secured by Paubox - HITRUST CSF certified https://www.paubox.com > ---- > > > > ---------- Forwarded message ---------- > From: Greg Dobbin > To: Nancy.Schmitt at mercyhealth.com, histonet at lists.utsouthwestern.edu > Cc: > Bcc: > Date: Tue, 8 Mar 2022 16:00:14 -0400 > Subject: Re: [Histonet] formalin in OR > Hi Nancy, > All routine specimens in our hospital are placed in formalin in the OR. > Breast lumps and mastectomy specimens are sent up fresh so that they arrive > STAT (to minimize cold ischemic times) and lymph nodes for lymphoma > protocol are also sent up fresh. [*they actually send sentinel nodes up > fresh too- it was just less confusing*] > > Diagnostic Imaging will place routine needle core biopsies directly in > formalin (e.g needle cores of breast, prostate, non-lympoma lymph nodes and > other misc. tissue masses). Lymph node cores for lymphoma protocol and > renal cores are sent to the lab fresh, in a container on saline soaked > Telfa pads. > > Endoscopy places all of their specimens directly in formalin. The derm > clinic will place all routine skins directly into formalin but specimens > destined for immunofluoresence are sent up to the lab fresh in a container > on saline soaked Telfa pads. EBUS specimens are split between Cytology and > Histology...the histo specimens go directly into formalin. > > Greg > > -- > *Greg Dobbin* > 1205 Pleasant Grove Rd > RR#2 York, > PE C0A 1P0 > > > *Everything in moderation...even moderation itself**!* > > > > > ---------- Forwarded message ---------- > From: Chenoa Hardwick > To: histonet at lists.utsouthwestern.edu > Cc: > Bcc: > Date: Tue, 8 Mar 2022 14:19:11 -0600 > Subject: [Histonet] posting > Please post: > > Job open - Salt Lake City, UT - Pathology Lab Manager > Relocation, sign on bonus available. > > Please contact Chenoa Hardwick at chenoa at pathologywatch.com 972-351-0774 > > *Chenoa Hardwick* > > VP of Laboratory Services > > email: chenoa at pathologywatch.com > > mobile: +1 972.351.0774 > > > > > > ---- > Secured by Paubox - HITRUST CSF certified > https://www.paubox.com > ---- > > > > ---------- Forwarded message ---------- > From: John Garratt > To: Nancy Schmitt , " > histonet at lists.utsouthwestern.edu" > Cc: > Bcc: > Date: Wed, 09 Mar 2022 01:28:25 +0000 > Subject: Re: [Histonet] formalin in OR > I would not normally promote a product but I have seen the TissueSafe and > SealSafe (Milestone) in use at a couple of hospitals for formalin > management in the OR and in the lab and was most impressed. > > John > > Sent from ProtonMail for iOS > > On Tue, Mar 8, 2022 at 9:54 AM, Nancy Schmitt via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hello- > > I would appreciate input on how you are getting formalin on to the > specimens: > > > > * Is it done in OR > > * Are specimens brough to pathology and add formalin there > > * If so - does lab or OR add the formalin > > * Other? > > Are you Joint Commission? > > Thank you! > > Nancy Schmitt MLT, HT(ASCP) > > Pathology Support Services > > Dubuque, IA > > > > Confidentiality Notice: > > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From Richard.Cartun at hhchealth.org Thu Mar 17 08:45:44 2022 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Thu, 17 Mar 2022 13:45:44 +0000 Subject: [Histonet] Histonet Digest, Vol 220, Issue 8 In-Reply-To: References: Message-ID: <7abeea919527492bac16c86e4e485690@hhchealth.org> Well stated Tony, and thank for all this useful information. I am hopeful that we can get expiration dates overturned here in the United States. In the past, I have recommended a "Stability Guarantee" date to replace the "Expiration Date". After that date passes it would be the responsibility of the performing laboratory to validate that the antibody is still working properly. And, as you pointed out, this is easily accomplished with the positive control which is run in parallel. Laboratories cannot no longer afford to discard expensive reagents, especially those that are not being produced any longer. Also, with the current staffing crisis in Histology/IHC laboratories and supply-chain issues we need to simplify our work environment. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Assistant Director, Anatomic Pathology Department of Pathology & Laboratory Medicine Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 (Fax) -----Original Message----- From: Tony Henwood (SCHN) via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 16, 2022 9:18 PM To: jayalakshmy p.s Cc: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Histonet Digest, Vol 220, Issue 8 EXTERNAL email from Outside HHC! Do NOT open attachments or click links from unknown senders. Hi Jayalakshmy, This our policy at the Kid's hospital in Sydney: Validation of Expired Antibodies Usually when a new concentrated antibody is received it will have an expiry date of around 2 years from receipt but usually we can continue to use antibodies well past this expiry date. If the antibody continues to stain control sections appropriately, with no loss of sensitivity and no increase in non-specific staining then its use should be continued. If positive control samples are deemed unsatisfactory, even if the antibody is within the manufacturer?s printed expiration date, evaluation of the clinical specimen is aborted and the test deemed invalid. The quality of the primary antibody is therefore not based on an expiration date, but rather on its performance on a case-by-case basis with appropriate positive and negative control samples (1). Several authors have investigated whether the shelf-life of diagnostic antibodies was longer than the expiry date on the label. They found them to work perfectly on routine histology sections (1-4). Monoclonal antibodies originally supplied as culture supernatants or as ascites (neat or diluted), of all isotypes, as well as all of the polyclonal antibodies, produced satisfactory staining irrespective of their age. Notable exceptions were ammonium-precipitated, IgM or conjugated antibodies. The policy at CHW is, when an antibody has reached past its expiry date, its control is tested to ensure that there has been no loss of sensitivity in the test. This is now controlled through iPassport, where a task is attached to the antibody requesting validation of control when the antibody is expired. This can be easily done by using the History Screen and looking for use of this antibody within the last two weeks. If results of control are acceptable, another task is instigated for 6 months hence. For antibody concentrates that are received without an expiry date, a verification is scheduled 12 months after receipt of the antibody. If an antibody fails to perform to expectations than a Corrective Action Request is instigated in iPassport and appropriate investigation is instituted. References: 1. Savage, E. C., & DeYoung, B. R. (2010). Antibody Expiration in the Context of Resource Limitation What Is the Evidence Basis?. American journal of clinical pathology, 134(1), 60-64. 2. Balaton, A. J., Drachenberg, C. B., Rucker, C., Vaury, P., & Papadimitriou, J. C. (1999). Satisfactory performance of primary antibodies beyond manufacturers' recommended expiration dates. Applied Immunohistochemistry & Molecular Morphology, 7(3), 221. 3. Argentieri, M. C., Pilla, D., Vanzati, A., Lonardi, S., Facchetti, F., Doglioni, C., & Cattoretti, G. (2013). Antibodies are forever: a study using 12-26?year?old expired antibodies. Histopathology, 63(6), 869-876. 4. Drachenberg, C. B., Papadimitriou, J. C., Balaton, A. J., & Vaury, P. (2001). The total test approach to standardization of immunohistochemistry. Archives of pathology & laboratory medicine, 125(4), 471-471. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: jayalakshmy p.s via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 10 March 2022 1:27 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 220, Issue 8 Hello all, I would like to know whether Immunohistochemistry markers can be used after its expiry date(atleast in resource poor countries). If yes, for how much period of time and what is the criteria to look for? Thanks and regards Jayalakshmy On Wed, Mar 9, 2022, 11:30 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!O2p8lTsV6LoMmBm9XUcfdnPxm396TKAYsfEW6qApeA3m1iuOjJSke7kfCQhuyFursm4$ > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Re: Post message to Histonet (Chenoa Hardwick) > 2. Re: formalin in OR (Greg Dobbin) > 3. posting (Chenoa Hardwick) > 4. Re: formalin in OR (John Garratt) > > > > ---------- Forwarded message ---------- > From: Chenoa Hardwick > To: histonet at lists.utsouthwestern.edu > Cc: > Bcc: > Date: Tue, 8 Mar 2022 12:17:13 -0600 > Subject: Re: [Histonet] Post message to Histonet Please post to > histonet: > > Job opening for histotechs (all shifts) and Pathology laboratory > manager position (Utah) > > Contact Chenoa at pathologywatch.com for inquiries. > > Thanks > > *Chenoa Hardwick* > > VP of Laboratory Services > > email: chenoa at pathologywatch.com > > mobile: +1 972.351.0774 > > > > > > On Tue, Mar 8, 2022 at 12:08 PM Chenoa Hardwick > > > wrote: > > > I would like to post a job opening on Histonet. > > > > Please see attached job description. > > > > Thanks > > > > *Chenoa Hardwick* > > > > VP of Laboratory Services > > > > email: chenoa at pathologywatch.com > > > > mobile: +1 972.351.0774 > > > > > > > > > > > ---- > Secured by Paubox - HITRUST CSF certified https://urldefense.com/v3/__https://www.paubox.com__;!!KCs9X-8!O2p8lTsV6LoMmBm9XUcfdnPxm396TKAYsfEW6qApeA3m1iuOjJSke7kfCQhuxFo2x94$ > ---- > > > > ---------- Forwarded message ---------- > From: Greg Dobbin > To: Nancy.Schmitt at mercyhealth.com, histonet at lists.utsouthwestern.edu > Cc: > Bcc: > Date: Tue, 8 Mar 2022 16:00:14 -0400 > Subject: Re: [Histonet] formalin in OR > Hi Nancy, > All routine specimens in our hospital are placed in formalin in the OR. > Breast lumps and mastectomy specimens are sent up fresh so that they arrive > STAT (to minimize cold ischemic times) and lymph nodes for lymphoma > protocol are also sent up fresh. [*they actually send sentinel nodes up > fresh too- it was just less confusing*] > > Diagnostic Imaging will place routine needle core biopsies directly in > formalin (e.g needle cores of breast, prostate, non-lympoma lymph nodes and > other misc. tissue masses). Lymph node cores for lymphoma protocol and > renal cores are sent to the lab fresh, in a container on saline soaked > Telfa pads. > > Endoscopy places all of their specimens directly in formalin. The derm > clinic will place all routine skins directly into formalin but specimens > destined for immunofluoresence are sent up to the lab fresh in a container > on saline soaked Telfa pads. EBUS specimens are split between Cytology and > Histology...the histo specimens go directly into formalin. > > Greg > > -- > *Greg Dobbin* > 1205 Pleasant Grove Rd > RR#2 York, > PE C0A 1P0 > > > *Everything in moderation...even moderation itself**!* > > > > > ---------- Forwarded message ---------- > From: Chenoa Hardwick > To: histonet at lists.utsouthwestern.edu > Cc: > Bcc: > Date: Tue, 8 Mar 2022 14:19:11 -0600 > Subject: [Histonet] posting > Please post: > > Job open - Salt Lake City, UT - Pathology Lab Manager > Relocation, sign on bonus available. > > Please contact Chenoa Hardwick at chenoa at pathologywatch.com 972-351-0774 > > *Chenoa Hardwick* > > VP of Laboratory Services > > email: chenoa at pathologywatch.com > > mobile: +1 972.351.0774 > > > > > > ---- > Secured by Paubox - HITRUST CSF certified > https://urldefense.com/v3/__https://www.paubox.com__;!!KCs9X-8!O2p8lTsV6LoMmBm9XUcfdnPxm396TKAYsfEW6qApeA3m1iuOjJSke7kfCQhuxFo2x94$ > ---- > > > > ---------- Forwarded message ---------- > From: John Garratt > To: Nancy Schmitt , " > histonet at lists.utsouthwestern.edu" > Cc: > Bcc: > Date: Wed, 09 Mar 2022 01:28:25 +0000 > Subject: Re: [Histonet] formalin in OR > I would not normally promote a product but I have seen the TissueSafe and > SealSafe (Milestone) in use at a couple of hospitals for formalin > management in the OR and in the lab and was most impressed. > > John > > Sent from ProtonMail for iOS > > On Tue, Mar 8, 2022 at 9:54 AM, Nancy Schmitt via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hello- > > I would appreciate input on how you are getting formalin on to the > specimens: > > > > * Is it done in OR > > * Are specimens brough to pathology and add formalin there > > * If so - does lab or OR add the formalin > > * Other? > > Are you Joint Commission? > > Thank you! > > Nancy Schmitt MLT, HT(ASCP) > > Pathology Support Services > > Dubuque, IA > > > > Confidentiality Notice: > > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!O2p8lTsV6LoMmBm9XUcfdnPxm396TKAYsfEW6qApeA3m1iuOjJSke7kfCQhuyFursm4$ > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!O2p8lTsV6LoMmBm9XUcfdnPxm396TKAYsfEW6qApeA3m1iuOjJSke7kfCQhuyFursm4$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!O2p8lTsV6LoMmBm9XUcfdnPxm396TKAYsfEW6qApeA3m1iuOjJSke7kfCQhuyFursm4$ This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!O2p8lTsV6LoMmBm9XUcfdnPxm396TKAYsfEW6qApeA3m1iuOjJSke7kfCQhuyFursm4$ Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From tbraud at holyredeemer.com Thu Mar 17 12:14:55 2022 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 17 Mar 2022 17:14:55 +0000 Subject: [Histonet] Stability Guarantee vs Expiration date Message-ID: <4b2ef2c471504e12b86434db5daae005@holyredeemer.com> Richard's suggestion is the most sensible suggestion that I've seen in a while, however, it means less money for reagent manufacturers, so I doubt we will ever see it come to pass. - Terri ------------------------------ Message: 2 Date: Thu, 17 Mar 2022 13:45:44 +0000 From: "Cartun, Richard" Well stated Tony, and thank for all this useful information. I am hopeful that we can get expiration dates overturned here in the United States. In the past, I have recommended a "Stability Guarantee" date to replace the "Expiration Date". After that date passes it would be the responsibility of the performing laboratory to validate that the antibody is still working properly. And, as you pointed out, this is easily accomplished with the positive control which is run in parallel. Laboratories cannot no longer afford to discard expensive reagents, especially those that are not being produced any longer. Also, with the current staffing crisis in Histology/IHC laboratories and supply-chain issues we need to simplify our work environment. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Assistant Director, Anatomic Pathology Department of Pathology & Laboratory Medicine Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 (Fax) From tony.henwood at health.nsw.gov.au Thu Mar 17 17:54:39 2022 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 17 Mar 2022 22:54:39 +0000 Subject: [Histonet] Histonet Digest, Vol 220, Issue 8 In-Reply-To: References: Message-ID: Hello Jayalakshmy, iPassport is our digital quality management system. It is a commercial product that allows us to collect data, record audits, document Corrective Actions, manage controlled documents (eg manuals), expiry data and verification of antibodies, staff competencies and training and equipment maintenance reports etc in order to aid us in meeting the ISO 15189 standard for laboratory accreditation. The ?History? screen is available on the Bond automated immunostainer. This is our policy for quality assurance of out-dated antibodies, so the mechanics are our department?s procedure. I did not try to make it generic (too little time to edit ? ). Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) | Principal Scientist; Adjunct Fellow, School of Medicine, University of Western Sydney; Visiting Lecturer, School of Life Sciences, Faculty of Science, University of Technology Sydney | Histopathology t: (02) 9845 3306 | e: tony.henwood at health.nsw.gov.au | w: www.schn.health.nsw.gov.au [http://res.schn.health.nsw.gov.au/signatures/facebook.gif] [http://res.schn.health.nsw.gov.au/signatures/twitter.gif] [http://res.schn.health.nsw.gov.au/signatures/chw.gif] Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia [http://res.schn.health.nsw.gov.au/signatures/shared_banner.jpg] ? Please consider the environment before printing this email. From: jayalakshmy p.s [mailto:psjayalakshmy at gmail.com] Sent: Friday, 18 March 2022 12:45 AM To: Tony Henwood (SCHN) Subject: Re: [Histonet] Histonet Digest, Vol 220, Issue 8 Hello Henwood, Thank you for the reply to my post with elaborate explanation and appropriate references. We doubted about the validity of using after expiry date. We keep onslide controls for every case. So with your suggestion we are confident of using it looking at the control. We, in developing country has not much finance to replenish the reagents past expiry date frequently when it is sparingly used. Sorry to say that I didnt understand the words ipassport & history screen. Can you elaborate a little more about these terms? Thanks & Regards Jayalakshmy On Thu, Mar 17, 2022, 6:48 AM Tony Henwood (SCHN) > wrote: Hi Jayalakshmy, This our policy at the Kid's hospital in Sydney: Validation of Expired Antibodies Usually when a new concentrated antibody is received it will have an expiry date of around 2 years from receipt but usually we can continue to use antibodies well past this expiry date. If the antibody continues to stain control sections appropriately, with no loss of sensitivity and no increase in non-specific staining then its use should be continued. If positive control samples are deemed unsatisfactory, even if the antibody is within the manufacturer?s printed expiration date, evaluation of the clinical specimen is aborted and the test deemed invalid. The quality of the primary antibody is therefore not based on an expiration date, but rather on its performance on a case-by-case basis with appropriate positive and negative control samples (1). Several authors have investigated whether the shelf-life of diagnostic antibodies was longer than the expiry date on the label. They found them to work perfectly on routine histology sections (1-4). Monoclonal antibodies originally supplied as culture supernatants or as ascites (neat or diluted), of all isotypes, as well as all of the polyclonal antibodies, produced satisfactory staining irrespective of their age. Notable exceptions were ammonium-precipitated, IgM or conjugated antibodies. The policy at CHW is, when an antibody has reached past its expiry date, its control is tested to ensure that there has been no loss of sensitivity in the test. This is now controlled through iPassport, where a task is attached to the antibody requesting validation of control when the antibody is expired. This can be easily done by using the History Screen and looking for use of this antibody within the last two weeks. If results of control are acceptable, another task is instigated for 6 months hence. For antibody concentrates that are received without an expiry date, a verification is scheduled 12 months after receipt of the antibody. If an antibody fails to perform to expectations then a Corrective Action Request is instigated in iPassport and appropriate investigation is instituted. References: 1. Savage, E. C., & DeYoung, B. R. (2010). Antibody Expiration in the Context of Resource Limitation What Is the Evidence Basis?. American journal of clinical pathology, 134(1), 60-64. 2. Balaton, A. J., Drachenberg, C. B., Rucker, C., Vaury, P., & Papadimitriou, J. C. (1999). Satisfactory performance of primary antibodies beyond manufacturers' recommended expiration dates. Applied Immunohistochemistry & Molecular Morphology, 7(3), 221. 3. Argentieri, M. C., Pilla, D., Vanzati, A., Lonardi, S., Facchetti, F., Doglioni, C., & Cattoretti, G. (2013). Antibodies are forever: a study using 12-26?year?old expired antibodies. Histopathology, 63(6), 869-876. 4. Drachenberg, C. B., Papadimitriou, J. C., Balaton, A. J., & Vaury, P. (2001). The total test approach to standardization of immunohistochemistry. Archives of pathology & laboratory medicine, 125(4), 471-471. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: jayalakshmy p.s via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 10 March 2022 1:27 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 220, Issue 8 Hello all, I would like to know whether Immunohistochemistry markers can be used after its expiry date(atleast in resource poor countries). If yes, for how much period of time and what is the criteria to look for? Thanks and regards Jayalakshmy On Wed, Mar 9, 2022, 11:30 PM > wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Re: Post message to Histonet (Chenoa Hardwick) > 2. Re: formalin in OR (Greg Dobbin) > 3. posting (Chenoa Hardwick) > 4. Re: formalin in OR (John Garratt) > > > > ---------- Forwarded message ---------- > From: Chenoa Hardwick > > To: histonet at lists.utsouthwestern.edu > Cc: > Bcc: > Date: Tue, 8 Mar 2022 12:17:13 -0600 > Subject: Re: [Histonet] Post message to Histonet Please post to > histonet: > > Job opening for histotechs (all shifts) and Pathology laboratory > manager position (Utah) > > Contact Chenoa at pathologywatch.com for inquiries. > > Thanks > > *Chenoa Hardwick* > > VP of Laboratory Services > > email: chenoa at pathologywatch.com > > > mobile: +1 972.351.0774 > > > > > > On Tue, Mar 8, 2022 at 12:08 PM Chenoa Hardwick > > > > wrote: > > > I would like to post a job opening on Histonet. > > > > Please see attached job description. > > > > Thanks > > > > *Chenoa Hardwick* > > > > VP of Laboratory Services > > > > email: chenoa at pathologywatch.com > > > > > mobile: +1 972.351.0774 > > > > > > > > > > > ---- > Secured by Paubox - HITRUST CSF certified https://www.paubox.com > ---- > > > > ---------- Forwarded message ---------- > From: Greg Dobbin > > To: Nancy.Schmitt at mercyhealth.com, histonet at lists.utsouthwestern.edu > Cc: > Bcc: > Date: Tue, 8 Mar 2022 16:00:14 -0400 > Subject: Re: [Histonet] formalin in OR > Hi Nancy, > All routine specimens in our hospital are placed in formalin in the OR. > Breast lumps and mastectomy specimens are sent up fresh so that they arrive > STAT (to minimize cold ischemic times) and lymph nodes for lymphoma > protocol are also sent up fresh. [*they actually send sentinel nodes up > fresh too- it was just less confusing*] > > Diagnostic Imaging will place routine needle core biopsies directly in > formalin (e.g needle cores of breast, prostate, non-lympoma lymph nodes and > other misc. tissue masses). Lymph node cores for lymphoma protocol and > renal cores are sent to the lab fresh, in a container on saline soaked > Telfa pads. > > Endoscopy places all of their specimens directly in formalin. The derm > clinic will place all routine skins directly into formalin but specimens > destined for immunofluoresence are sent up to the lab fresh in a container > on saline soaked Telfa pads. EBUS specimens are split between Cytology and > Histology...the histo specimens go directly into formalin. > > Greg > > -- > *Greg Dobbin* > 1205 Pleasant Grove Rd > RR#2 York, > PE C0A 1P0 > > > *Everything in moderation...even moderation itself**!* > > > > > ---------- Forwarded message ---------- > From: Chenoa Hardwick > > To: histonet at lists.utsouthwestern.edu > Cc: > Bcc: > Date: Tue, 8 Mar 2022 14:19:11 -0600 > Subject: [Histonet] posting > Please post: > > Job open - Salt Lake City, UT - Pathology Lab Manager > Relocation, sign on bonus available. > > Please contact Chenoa Hardwick at chenoa at pathologywatch.com 972-351-0774 > > *Chenoa Hardwick* > > VP of Laboratory Services > > email: chenoa at pathologywatch.com > > > mobile: +1 972.351.0774 > > > > > > ---- > Secured by Paubox - HITRUST CSF certified > https://www.paubox.com > ---- > > > > ---------- Forwarded message ---------- > From: John Garratt > > To: Nancy Schmitt >, " > histonet at lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Wed, 09 Mar 2022 01:28:25 +0000 > Subject: Re: [Histonet] formalin in OR > I would not normally promote a product but I have seen the TissueSafe and > SealSafe (Milestone) in use at a couple of hospitals for formalin > management in the OR and in the lab and was most impressed. > > John > > Sent from ProtonMail for iOS > > On Tue, Mar 8, 2022 at 9:54 AM, Nancy Schmitt via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hello- > > I would appreciate input on how you are getting formalin on to the > specimens: > > > > * Is it done in OR > > * Are specimens brough to pathology and add formalin there > > * If so - does lab or OR add the formalin > > * Other? > > Are you Joint Commission? > > Thank you! > > Nancy Schmitt MLT, HT(ASCP) > > Pathology Support Services > > Dubuque, IA > > > > Confidentiality Notice: > > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From carl.hobbs at kcl.ac.uk Fri Mar 18 14:56:46 2022 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 18 Mar 2022 19:56:46 +0000 Subject: [Histonet] Stability Guarantee vs Expiration date Message-ID: Good info I have always made 1/10 stocks of my abs ( in TBS/BSA/azide) and frozen aliquoted neat stocks. For eg: I still use a 10 yr old "Dako" anti GFAP at 1/5000 ( stock 4C 1/10) Sure, I am in research Sure, I always add a positive control Today I used a 5 yr old 1/10 4C of anti PCNA ( Abcam ab29)...worked as well as when I 1st made the 1/10 stock Sure....one has to keep checking by adding controls ( a known positive like gut so that if crypt nuclei are exclusively +ve...OK) Mind you.....PCNA is NOT a great prolif marker : far better to use anti Ki67 ( half-life difference) However, I wanted to see if Ki67/PCNA was good for worm Pwax sections ( former -ve, latter TOO positive) Caveat: stored positive control sections can "go off" Some call it "oxidation" of epitopes It happens to some epitopes/ags Best wishes, Histonet Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6810 From relia1 at earthlink.net Tue Mar 22 13:30:44 2022 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 22 Mar 2022 14:30:44 -0400 Subject: [Histonet] Info on State and Regional Society Meetings and some exciting new job opportunities. Message-ID: <001e01d83e1a$f2541c60$d6fc5520$@earthlink.net> Hi Histopeeps, I hope you are having a great day! I have heard on the grapevine that some of the state and regional societies are having their meetings this year. I would love to share the information with all of your fellow histopeeps. So... This is a call for information regarding state and regional meetings to be published in the 03/30/2022 RELIA Careers Bulletin (free posting) If you would like the link to your website and or meeting info to be mentioned in the 03/30/2022 RELIA Careers Bulletin please email it to RELIA1 at earthlink.net by 03/29/2022. Sometimes your next career move is just a matter of being in the RIGHT place at the RIGHT time and ready for the RIGHT opportunity. Right Place, Right Time, Right Opportunity! Odds are that if you are contemplating a job change for whatever reason - better compensation, a more desirable location or more challenging work, you don't have the time to do a job search. I would like to offer my help... I have clients located throughout the country. All of the jobs that I represent are full time permanent positions with excellent compensation, benefits and relocation/sign-on bonuses. I will assist you with your resume, coordination of interviews and coaching throughout the process. My services are FREE of charge to you. Here is the latest update of the positions I am most excited to represent. If you are interested in any of these positions - give me a call toll free at 866-607-3542 or shoot me an e-mail at relia1 at earthlink.net and let's discuss it. Whether you are looking for a new position today, tomorrow or 6 months from now, it is never too early to have me keep a watch out for that perfect job for you! I have fantastic opportunities in: * Management * Immunohistochemistry * Research * Training * Cancer Diagnostics * Private Pathology Laboratories *I have positions on all shifts day, mid and night shifts!* These fantastic opportunities are located in: Utah: * Salt Lake City, UT Texas: * Abilene, TX Colorado: * Denver, CO Arizona: * Phoenix, AZ Tennessee: * Knoxville, TN area Florida: * Sarasota, FL * Boynton Beach, FL * Port Charlotte, FL * Ft. Myers, FL * Panama City, FL California: * Orange County, CA * San Diego, CA * Stockton, CA New York: * Middletown, NY If you are looking but not in one of these areas please let me know so I can pinpoint opportunities for you! please don't forget this is call for information regarding state and regional meetings to be published in the 03/30/2022 RELIA Careers Bulletin (free posting) If you would like the link to your website and or meeting info to be mentioned in the 03/30/2022 RELIA Careers Bulletin please email it to RELIA1 at earthlink.net by 03/29/2022. Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions From koellingr at comcast.net Tue Mar 22 14:46:26 2022 From: koellingr at comcast.net (RAY KOELLING) Date: Tue, 22 Mar 2022 12:46:26 -0700 (PDT) Subject: [Histonet] test Message-ID: <1771139662.9856.1647978387176@connect.xfinity.com> Sorry, just a test. Problematic with messages whether receiving or going. Ray Koelling Fair Director, Eastern Washington Regional Science and Engineering Fair Board Member, Washington State Science and Engineering Fair From relia1 at earthlink.net Wed Mar 23 10:28:35 2022 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 23 Mar 2022 11:28:35 -0400 Subject: [Histonet] Info on State and Regional Society Meetings and some exciting new job opportunities. Message-ID: <00c601d83eca$aa76fbf0$ff64f3d0$@earthlink.net> Hi Histopeeps, I hope you are having a great day! I have heard on the grapevine that some of the state and regional societies are having their meetings this year. I would love to share the information with all of your fellow histopeeps. So? This is a call for information regarding state and regional meetings to be published in the 03/30/2022 RELIA Careers Bulletin (free posting) If you would like the link to your website and or meeting info to be mentioned in the 03/30/2022 RELIA Careers Bulletin please email it to mailto:RELIA1 at earthlink.net by 03/29/2022. Sometimes your next career move is just a matter of being in the RIGHT place at the RIGHT time and ready for the RIGHT opportunity. Right Place, Right Time, Right Opportunity! Odds are that if you are contemplating a job change for whatever reason ? better compensation, a more desirable location or more challenging work, you don?t have the time to do a job search. I would like to offer my help? I have clients located throughout the country. All of the jobs that I represent are full time permanent positions with excellent compensation, benefits and relocation/sign-on bonuses. I will assist you with your resume, coordination of interviews and coaching throughout the process. My services are FREE of charge to you. Here is the latest update of the positions I am most excited to represent. If you are interested in any of these positions ? give me a call toll free at 866-607-3542 or shoot me an e-mail at mailto:relia1 at earthlink.net and let?s discuss it. Whether you are looking for a new position today, tomorrow or 6 months from now, it is never too early to have me keep a watch out for that perfect job for you! I have fantastic opportunities in: ? Management ? Immunohistochemistry ? Research ? Training ? Cancer Diagnostics ? Private Pathology Laboratories *I have positions on all shifts day, mid and night shifts!* These fantastic opportunities are located in: Utah: ? Salt Lake City, UT Texas: ? Abilene, TX Colorado: ? Denver, CO Arizona: ? Phoenix, AZ Tennessee: ? Knoxville, TN area Florida: ? Sarasota, FL ? Boynton Beach, FL ? Port Charlotte, FL ? Ft. Myers, FL ? Panama City, FL California: ? Orange County, CA ? San Diego, CA ? Stockton, CA New York: ? Middletown, NY If you are looking but not in one of these areas please let me know so I can pinpoint opportunities for you! Please don?t forget this is call for information regarding state and regional meetings to be published in the 03/30/2022 RELIA Careers Bulletin (free posting) If you would like the link to your website and or meeting info to be mentioned in the 03/30/2022 RELIA Careers Bulletin please email it to mailto:RELIA1 at earthlink.net by 03/29/2022. Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:? (407)353-5070 FAX:???? (407)678-2788 E-mail: mailto:relia1 at earthlink.net?? https://www.facebook.com/RELIASolutionsforhistologyprofessionals http://www.linkedin.com/in/reliasolutions From bcooper at chla.usc.edu Wed Mar 30 12:57:23 2022 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 30 Mar 2022 17:57:23 +0000 Subject: [Histonet] Melanoma Control Blocks? Message-ID: <1a9888db4d8a4926ac0740a9a601fecd@chla.usc.edu> Good morning Histonet! Does any of you have any melanoma control blocks you'd be willing to share? We have a bunch of other controls we can trade for: CMV, EBV, Pneumocystis, HSV 1 or HSV2 just to name a few. Please message me offline if you're able to help! Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From gu.lang at gmx.at Thu Mar 31 04:47:16 2022 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 31 Mar 2022 11:47:16 +0200 Subject: [Histonet] Looking for old Microm-bladeholders Message-ID: <000001d844e4$4ee01450$eca03cf0$@gmx.at> Dear histonetters, I am looking for old Microm bladeholders for sliding-microtomes. Those ones, that have a shorter coverplate than the standard blade. We prefer them for working, but they aren't sold any more. I would be happy for any idea or contact - preferable in Europe. Maybe someone has an unused bladeholder in the lab-storage. Thanks in advance Lang Gudrun Austria From bcooper at chla.usc.edu Thu Mar 31 11:04:57 2022 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Thu, 31 Mar 2022 16:04:57 +0000 Subject: [Histonet] Melanoma Control Blocks? In-Reply-To: <1a9888db4d8a4926ac0740a9a601fecd@chla.usc.edu> References: <1a9888db4d8a4926ac0740a9a601fecd@chla.usc.edu> Message-ID: <2c42641f699d4b2eb99674644e27016a@chla.usc.edu> Good morning Histonet! Just wanted to let you know that we've received a lot of offers for help on melanoma controls since my email yesterday. Thank you to all who have responded. I think we will likely have melanoma controls for many years to come if these all pan out! Much appreciated Histonet; when one of us needs help, this is always such a valuable resource. Happy almost Friday! Thanks, Brian -----Original Message----- From: Cooper, Brian via Histonet Sent: Wednesday, March 30, 2022 10:57 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Melanoma Control Blocks? (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. Good morning Histonet! Does any of you have any melanoma control blocks you'd be willing to share? We have a bunch of other controls we can trade for: CMV, EBV, Pneumocystis, HSV 1 or HSV2 just to name a few. Please message me offline if you're able to help! Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. 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