From PKRichar at gundersenhealth.org Tue Feb 1 12:44:17 2022 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Tue, 1 Feb 2022 18:44:17 +0000 Subject: [Histonet] Slide printers Message-ID: Looking for input on the Epso slide printers. What do you like and what would you change? Best Wishes Pam ~ National Histology Professionals Day 3/10/22 Pathologists' Assistant Day 4/14/2022 Medical Laboratory Professionals Week April 25-29, 2022 National Cytotechnology Day 5/13/2022 +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org From jmacdonald at mtsac.edu Tue Feb 1 17:06:57 2022 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Tue, 1 Feb 2022 23:06:57 +0000 Subject: [Histonet] Detection Systems Message-ID: Are labs using LSAB detection? I know the polymers are more popular, but was wondering if avidin/biotin is still being used? Thanks, Jennifer From carl.hobbs at kcl.ac.uk Wed Feb 2 13:29:23 2022 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Wed, 2 Feb 2022 19:29:23 +0000 Subject: [Histonet] Detection Systems Message-ID: Hi I'm a loner Biomed Scientist ( Histology specialist) in Research I use stABCpx-DAB regularly Yes, polymer kits are quicker and very sensitive. However, I have tested 3 popular such kits and they ONLY work well( in my hands) if you also use their DAB: I tested this by substituting my DAB for theirs ( and vice versa) and got the same signal strength as my "LSAB" std method I also used my LSAB/their DAB and got greater sensitivity So, imho....it's the DAB that gives greater sensitivity thus more dilute most expensive primary abs. Sure, if time/simplicity is of the essence...Polymer kits are the current "best" However....for an extra max 60 mins...I just add Imidazole to my DAB visualisation solution after stABCpx detection That gives me identical sensitivity to polmer kits at much less cost. Sure, I can "play"...if you are in Diagnostic Histopath......you can't. Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6810 From modz9636 at gmail.com Thu Feb 3 11:43:05 2022 From: modz9636 at gmail.com (M.O.) Date: Thu, 3 Feb 2022 09:43:05 -0800 Subject: [Histonet] fresh frozen OCT samples, high quality RNA extraction Message-ID: Hello, This might be a longshot, but has anyone had success in extracting high quality RNA from fresh frozen OCT samples/sections? I'm trying to get high RINs from human samples and succeeded only once (out of what seems to be 1 million attempts). I've trimmed off as much OCT as possible, and even picked the sample out of the OCT when sectioning. Any tips or hints would be most appreciated. Sincerely, Merissa From cmmathis at wakehealth.edu Thu Feb 3 15:15:35 2022 From: cmmathis at wakehealth.edu (Cathy M. Mathis/Comparative Medicine) Date: Thu, 3 Feb 2022 21:15:35 +0000 Subject: [Histonet] 5 Yr old Leica ST5020 is down Message-ID: Hello Histology friends, We are a research lab with a 5 year old Leica ST5020 autostainer that looks like new. We run less than 5000 slides a year on it. We noticed that (of course just after the warranty ended) that one of the water rinse stations stayed full of water all the time, even when the instrument was off. It may have been like this from the beginning and we just did not notice it. Leica quoted a $6000 repair but said we could run it as is. So, we skipped the repair and went on using it. Now the instrument is completely down and the repair is a new "slave controller board" for $17,600, stepper motor for arm, etc. with total quote for over $23,800. Has anyone else had major issues with this instrument over time. We are trying to decide whether to fix it or go for a new stainer. I'm kinda wondering if we got a lemon. Thank you for your time, Cathy Cathy M. Mathis Lab Technician IV Dept. of Pathology \ Comparative Medicine Wake Forest School of Medicine p 336.716.1538 \ f 336.716.1515 cmmathis at wakehealth.edu www.wakehealth.edu From histotrek at gmail.com Thu Feb 3 19:20:33 2022 From: histotrek at gmail.com (HistoTrek) Date: Thu, 3 Feb 2022 20:20:33 -0500 Subject: [Histonet] For those of you interested in travel work! Message-ID: Hi histology friends! With all the staffing shortages, overtime, and pandemic nonsense, it's very likely that you?ve heard about travel work a handful of times. I am a travel histotechnologist who started traveling after COVID19 hit and the dynamic in my hospital lab changed A LOT. I was ready for something new! I have been working on a *Getting Started with Travel Work* series on my blog and would like to invite everyone interested in travel work to check it out! I publish every Wednesday, and the getting started series is nearly complete. I?d love to get some feedback from the community about what is important to you, what you?d like to read about, and how I can help make the transition into travel work a little easier. Please check out the website at https://www.histotrek.com, the travel series at https://histotrek.com/start-here/, and let me know your feedback! I am on all social media platforms at HistoTrek, or you can email me directly at contact at histotrek.com. From mim9060 at nyp.org Fri Feb 4 12:04:35 2022 From: mim9060 at nyp.org (Maimone-Schoen, Michele) Date: Fri, 4 Feb 2022 18:04:35 +0000 Subject: [Histonet] Positions Available at New York Presbyterian Hospital/Weill Cornell Message-ID: <324d36b7c2f04d17b35668c857956946@nyp.org> We have the following Histotech positions available at NYPWC in New York City: Evening Shift - 2 p 10 p or 3 p - 11 p Night Shift (2) - varies: 10 p - 6 a ; 11 p - 7 a ; 12 a - 8 a Sign on Bonus Please reach out to me or go to our Web Site - NYP.Org/careers thanks Michele Maimone-Schoen, MS Manager, Anatomic Pathology New York-Presbyterian Hospital 525 East 68th Street, Starr 1003 New York, NY 10065 212-746-2633 (Office) 212-746-5007 (Fax) 646-856-1738 (Cell) Be Amazing! ---------------------------------------------------------------------- This electronic message is intended to be for the use only of the named recipient, and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. Thank you. code:d34y From amosbrooks at gmail.com Sat Feb 5 12:22:03 2022 From: amosbrooks at gmail.com (Amos Brooks) Date: Sat, 5 Feb 2022 13:22:03 -0500 Subject: [Histonet] Detection Systems In-Reply-To: References: Message-ID: Hi, I use polymers regularly, but keep a small kit of LSAB or the like on hand for oddball solutions like when someone gives me a biotinylated antibody or an antibody with next to no information about what it was raised in. I don't mind a biotinylated antibody all that much, but no species info is really frustrating. It has it's uses on occasion. Amos Brooks > ------------------------------ > Message: 2 > Date: Tue, 1 Feb 2022 23:06:57 +0000 > From: "Mac Donald, Jennifer" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Detection Systems > Message-ID: > < > CH0P223MB0345D83872230694C4CD154CC1269 at CH0P223MB0345.NAMP223.PROD.OUTLOOK.COM > > > > Content-Type: text/plain; charset="us-ascii" > > Are labs using LSAB detection? I know the polymers are more popular, but > was wondering if avidin/biotin is still being used? > Thanks, > Jennifer > > From jmacdonald at mtsac.edu Sat Feb 5 15:26:34 2022 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Sat, 5 Feb 2022 21:26:34 +0000 Subject: [Histonet] Detection Systems In-Reply-To: References: Message-ID: Thank you. Get Outlook for iOS ________________________________ From: Amos Brooks via Histonet Sent: Saturday, February 5, 2022 10:22:03 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Detection Systems EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Hi, I use polymers regularly, but keep a small kit of LSAB or the like on hand for oddball solutions like when someone gives me a biotinylated antibody or an antibody with next to no information about what it was raised in. I don't mind a biotinylated antibody all that much, but no species info is really frustrating. It has it's uses on occasion. Amos Brooks > ------------------------------ > Message: 2 > Date: Tue, 1 Feb 2022 23:06:57 +0000 > From: "Mac Donald, Jennifer" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Detection Systems > Message-ID: > < > CH0P223MB0345D83872230694C4CD154CC1269 at CH0P223MB0345.NAMP223.PROD.OUTLOOK.COM > > > > Content-Type: text/plain; charset="us-ascii" > > Are labs using LSAB detection? I know the polymers are more popular, but > was wondering if avidin/biotin is still being used? > Thanks, > Jennifer > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cjmacdonald%40mtsac.edu%7Ce3286475aef14a26165308d9e8d48521%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637796821730008483%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=ZzcJDQ%2FtKC8oY3%2BtrZqnZ5FrhJry9mhTeuGYiFbmdto%3D&reserved=0 From carl.hobbs at kcl.ac.uk Sun Feb 6 13:32:49 2022 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sun, 6 Feb 2022 19:32:49 +0000 Subject: [Histonet] Detection Systems (Mac Donald, Jennifer) Message-ID: As I stated: Polymer systems only work GREAT when you also use the DAB in the kit You pay dearly for it.... Check out my comparison in Histonet Images Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6810 From sprice2003 at gmail.com Sun Feb 6 14:09:15 2022 From: sprice2003 at gmail.com (Sally Price) Date: Sun, 6 Feb 2022 15:09:15 -0500 Subject: [Histonet] Detection Systems In-Reply-To: References: Message-ID: Like Amos, I have extensive experience in working with biotin/streptavidin (BSA) detection systems, and use them primarily because, in my analyses, such reagents cost about half as much as polymers kits in the same volume containers. In addition, knowing that endogenous biotin can be problematic in a small number of different tissues, our (clinical) lab simply avoids using BSA in those situations. As required by CAP standards, our IHC policy acknowledges that we?ve conducted the necessary testing to determine if/when endogenous biotin makes interpretation challenging, and refrain from doing so when applicable. Finally, although I don?t agree with Carl?s belief that one MUST use the DAB chromogen+substrate components offered by the same vendor as the detection components, we DO purchase such a reagents from the same manufacturer simply because they?re more inclined to assist us in troubleshooting if we run into a problem with EITHER reagent. Sally On Sun, Feb 6, 2022 at 2:40 PM Hobbs, Carl via Histonet < histonet at lists.utsouthwestern.edu> wrote: > As I stated: > Polymer systems only work GREAT when you also use the DAB in the kit > You pay dearly for it.... > Check out my comparison in Histonet Images > Carl > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > > > 020 7848 6810 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Sally Price From mjdessoye at commonwealthhealth.net Mon Feb 7 09:06:19 2022 From: mjdessoye at commonwealthhealth.net (Michael Dessoye) Date: Mon, 7 Feb 2022 10:06:19 -0500 Subject: [Histonet] [EXTERNAL] Histonet Digest, Vol 219, Issue 3 In-Reply-To: References: Message-ID: Based on my experience with this stainer, you may want to think about replacing. The water valve assembly/manifold (and associated parts) are notorious for having expensive parts fail, especially the very expensive manifold. I'd look elsewhere for a replacement. ---------- Forwarded message ---------- From: "Cathy M. Mathis/Comparative Medicine" To: "histonet at lists.utsouthwestern.edu" Cc: Bcc: Date: Thu, 3 Feb 2022 21:15:35 +0000 Subject: [Histonet] 5 Yr old Leica ST5020 is down Hello Histology friends, We are a research lab with a 5 year old Leica ST5020 autostainer that looks like new. We run less than 5000 slides a year on it. We noticed that (of course just after the warranty ended) that one of the water rinse stations stayed full of water all the time, even when the instrument was off. It may have been like this from the beginning and we just did not notice it. Leica quoted a $6000 repair but said we could run it as is. So, we skipped the repair and went on using it. Now the instrument is completely down and the repair is a new "slave controller board" for $17,600, stepper motor for arm, etc. with total quote for over $23,800. Has anyone else had major issues with this instrument over time. We are trying to decide whether to fix it or go for a new stainer. I'm kinda wondering if we got a lemon. Thank you for your time, Cathy Cathy M. Mathis Lab Technician IV Dept. of Pathology \ Comparative Medicine Wake Forest School of Medicine p 336.716.1538 \ f 336.716.1515 cmmathis at wakehealth.edu www.wakehealth.edu On Fri, Feb 4, 2022 at 1:00 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. 5 Yr old Leica ST5020 is down > (Cathy M. Mathis/Comparative Medicine) > 2. For those of you interested in travel work! (HistoTrek) > > > > ---------- Forwarded message ---------- > From: "Cathy M. Mathis/Comparative Medicine" > To: "histonet at lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Thu, 3 Feb 2022 21:15:35 +0000 > Subject: [Histonet] 5 Yr old Leica ST5020 is down > Hello Histology friends, > We are a research lab with a 5 year old Leica ST5020 autostainer that > looks like new. We run less than 5000 slides a year on it. We noticed > that (of course just after the warranty ended) that one of the water rinse > stations stayed full of water all the time, even when the instrument was > off. It may have been like this from the beginning and we just did not > notice it. Leica quoted a $6000 repair but said we could run it as is. > So, we skipped the repair and went on using it. > Now the instrument is completely down and the repair is a new "slave > controller board" for $17,600, stepper motor for arm, etc. with total quote > for over $23,800. Has anyone else had major issues with this instrument > over time. We are trying to decide whether to fix it or go for a new > stainer. I'm kinda wondering if we got a lemon. > Thank you for your time, > Cathy > > Cathy M. Mathis > Lab Technician IV > Dept. of Pathology \ Comparative Medicine > Wake Forest School of Medicine > p 336.716.1538 \ f 336.716.1515 > cmmathis at wakehealth.edu > www.wakehealth.edu > > > > > > > > ---------- Forwarded message ---------- > From: HistoTrek > To: histonet at lists.utsouthwestern.edu > Cc: > Bcc: > Date: Thu, 3 Feb 2022 20:20:33 -0500 > Subject: [Histonet] For those of you interested in travel work! > Hi histology friends! > > With all the staffing shortages, overtime, and pandemic nonsense, it's > very likely that you?ve heard about travel work a handful of times. > > I am a travel histotechnologist who started traveling after COVID19 hit > and the dynamic in my hospital lab changed A LOT. I was ready for > something new! > > I have been working on a *Getting Started with Travel Work* series on my > blog and would like to invite everyone interested in travel work to > check it out! > > I publish every Wednesday, and the getting started series is nearly > complete. > > I?d love to get some feedback from the community about what is important > to you, what you?d like to read about, and how I can help make the > transition into travel work a little easier. > > Please check out the website at https://www.histotrek.com, the travel > series at https://histotrek.com/start-here/, and let me know your > feedback! I am on all social media platforms at HistoTrek, or you can > email me directly at contact at histotrek.com. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Michael J. Dessoye, M.S. | Laboratory Supervisor | Commonwealth Health Laboratory Services | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 From jaylundgren at gmail.com Mon Feb 7 09:13:10 2022 From: jaylundgren at gmail.com (Jay Lundgren) Date: Mon, 7 Feb 2022 09:13:10 -0600 Subject: [Histonet] 5 Yr old Leica ST5020 is down In-Reply-To: References: Message-ID: > > Never buy an instrument without an extended service contract. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Richard.Cartun at hhchealth.org Mon Feb 7 09:14:27 2022 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 7 Feb 2022 15:14:27 +0000 Subject: [Histonet] Pneumocystis Control Tissue Message-ID: <38e0d93ea30f485a87944bd630ee86ee@hhchealth.org> I apologize .... someone contacted me several weeks ago about the availability of Pneumocystis control tissue, and I misplaced his name and contact information. Please contact me again if you are still in need of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Assistant Director, Anatomic Pathology Department of Pathology & Laboratory Medicine Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 (Fax) This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From eddessa at emory.edu Mon Feb 7 10:52:15 2022 From: eddessa at emory.edu (Dessasau, Evan D.) Date: Mon, 7 Feb 2022 16:52:15 +0000 Subject: [Histonet] FW: [External] Job ad in Athens, GA. Message-ID: Hi All, I'm posting this for a PI that has a position to fill Athens, Georgia. The Hyperlink can be copied and pasted to the search bar. Please read below. From: sanjeev.gumber at boehringer-ingelheim.com Sent: Monday, February 07, 2022 8:36 AM Subject: [External] Job ad Hi , I am looking to fill a histology/molecular pathology position in our lab. The position is based in Athens, GA. Job Description - US_Scientist III, Molecular Pathology (220976) (taleo.net) Thanks, Sanjeev This e-mail is confidential and may also be legally privileged. If you are not the intended recipient please reply to sender, delete the e-mail and do not disclose its contents to any person. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. From Jessica.Piche at wtbyhosp.org Tue Feb 8 06:49:39 2022 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Tue, 8 Feb 2022 12:49:39 +0000 Subject: [Histonet] procedure manuals for special stains Message-ID: Good Morning, I hope everyone is doing well. We have the Ventana Roche Benchmark special stainer. How is everyone doing their procedure manual for the actual stain protocols? What is the CAP requirement? Thank you, Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 From Jeffrey.Rinker at SanfordHealth.org Thu Feb 10 10:22:13 2022 From: Jeffrey.Rinker at SanfordHealth.org (Rinker,Jeffrey) Date: Thu, 10 Feb 2022 16:22:13 +0000 Subject: [Histonet] Tiny folds in colon bx Message-ID: I have been having a terrible time with small folds in my colon bxs. They tend to be perpendicular to the cutting edge and i have tried all the standard ways to get rid of them. My Dr says that the sections are fine and that they don't effect the diagnosis but i would really like to figure out why hey are there. ----------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain privileged and confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From LNormington at uwhealth.org Thu Feb 10 10:51:15 2022 From: LNormington at uwhealth.org (Normington, Lacy) Date: Thu, 10 Feb 2022 16:51:15 +0000 Subject: [Histonet] Flooring Message-ID: What does your institution do to mitigate paraffin collection on the floors? Do you strip the floors at defined periods of time? Do you use mats throughout entire laboratory, under microtomy stations? Currently our institution uses a sticky plastic green flooring, which is ripped up every quarter and replaced. However, during this time there are many spots which the plastic breaks down due to consistent activity over a spot. This requires the flooring to be scraped, stripped, etc. Thanks Lacy Normington UW Health From c.tague at pathologyarts.com Thu Feb 10 11:42:18 2022 From: c.tague at pathologyarts.com (Curt Tague) Date: Thu, 10 Feb 2022 17:42:18 +0000 Subject: [Histonet] Flooring In-Reply-To: References: Message-ID: We use some of this clear vinyl, like you'd see in a walk in fridge... get the wide stuff and cut it out to fit under a cutting station. It completely saves the floors and when it's trashed out we can just throw it away, get he floors cleaned and put some more down... works great for me. https://www.marinevinylfabric.com/products/clear-marine-vinyl?variant=39274692935764¤cy=USD&utm_medium=product_sync&utm_source=google&utm_content=sag_organic&utm_campaign=sag_organic&gclid=Cj0KCQiAjJOQBhCkARIsAEKMtO10i6dntCGz7nN7NIKDOxpa2a9Lp3o7EsglXSl9Mh-3TdVmL1pm0ugaAlvhEALw_wcB https://www.strip-curtains.com/proCat/bulkVinylRolls.php we use the 48" roll here I'm sure you can google other vendors too... best of luck! Curt -----Original Message----- From: Normington, Lacy via Histonet Sent: Thursday, February 10, 2022 8:51 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Flooring What does your institution do to mitigate paraffin collection on the floors? Do you strip the floors at defined periods of time? Do you use mats throughout entire laboratory, under microtomy stations? Currently our institution uses a sticky plastic green flooring, which is ripped up every quarter and replaced. However, during this time there are many spots which the plastic breaks down due to consistent activity over a spot. This requires the flooring to be scraped, stripped, etc. Thanks Lacy Normington UW Health _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=2t7_gY_QgCsQnEhIbW0hGqXohzrIjiJRpXu4w5m7YEQ&m=hWEl-W67C0HeYK26kkJwaxwYz25-7T9gHERINBtJ8wo&s=xJ1cq8UebwyPgqtjwY7XKdG0q7IfyadzT9FjxdFswX8&e= From tbraud at holyredeemer.com Thu Feb 10 12:08:41 2022 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 10 Feb 2022 18:08:41 +0000 Subject: [Histonet] paraffin on floors Message-ID: <48E053DDF6CE074DB6A7414BA05403F8023419F753@HRHEX02-HOS.holyredeemer.local> After a ba-zillion years in Histology, I've found there is no substitution for having the floors stripped every 2 weeks. The trick is NOT to apply floor wax after stripping. We've also been currently using mats in key areas where the most traffic is. This prevents wax from being ground into the floor and becoming slippery. The mats are changed out every 2 weeks, too. The techs are expected to scrape up excess floor paraffin at the end of their shift or at the end of daily cutting. I hope this helps. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Thursday, February 10, 2022 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 219, Issue 8 CAUTION: This email originated from outside Redeemer Health. Do not click links or open attachments unless you recognize the sender and know the content is safe. Contact our IT Support Center at 215-938-3900 with questions. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Tiny folds in colon bx (Rinker,Jeffrey) 2. Flooring (Normington, Lacy) 3. Re: Flooring (Curt Tague) ---------------------------------------------------------------------- Message: 1 Date: Thu, 10 Feb 2022 16:22:13 +0000 From: "Rinker,Jeffrey" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Tiny folds in colon bx Message-ID: Content-Type: text/plain; charset="iso-8859-1" I have been having a terrible time with small folds in my colon bxs. They tend to be perpendicular to the cutting edge and i have tried all the standard ways to get rid of them. My Dr says that the sections are fine and that they don't effect the diagnosis but i would really like to figure out why hey are there. ----------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain privileged and confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 2 Date: Thu, 10 Feb 2022 16:51:15 +0000 From: "Normington, Lacy" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Flooring Message-ID: Content-Type: text/plain; charset="us-ascii" What does your institution do to mitigate paraffin collection on the floors? Do you strip the floors at defined periods of time? Do you use mats throughout entire laboratory, under microtomy stations? Currently our institution uses a sticky plastic green flooring, which is ripped up every quarter and replaced. However, during this time there are many spots which the plastic breaks down due to consistent activity over a spot. This requires the flooring to be scraped, stripped, etc. Thanks Lacy Normington UW Health ------------------------------ Message: 3 Date: Thu, 10 Feb 2022 17:42:18 +0000 From: Curt Tague To: "Normington, Lacy" , "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] Flooring Message-ID: Content-Type: text/plain; charset="us-ascii" We use some of this clear vinyl, like you'd see in a walk in fridge... get the wide stuff and cut it out to fit under a cutting station. It completely saves the floors and when it's trashed out we can just throw it away, get he floors cleaned and put some more down... works great for me. https://www.marinevinylfabric.com/products/clear-marine-vinyl?variant=39274692935764¤cy=USD&utm_medium=product_sync&utm_source=google&utm_content=sag_organic&utm_campaign=sag_organic&gclid=Cj0KCQiAjJOQBhCkARIsAEKMtO10i6dntCGz7nN7NIKDOxpa2a9Lp3o7EsglXSl9Mh-3TdVmL1pm0ugaAlvhEALw_wcB https://www.strip-curtains.com/proCat/bulkVinylRolls.php we use the 48" roll here I'm sure you can google other vendors too... best of luck! Curt -----Original Message----- From: Normington, Lacy via Histonet Sent: Thursday, February 10, 2022 8:51 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Flooring What does your institution do to mitigate paraffin collection on the floors? Do you strip the floors at defined periods of time? Do you use mats throughout entire laboratory, under microtomy stations? Currently our institution uses a sticky plastic green flooring, which is ripped up every quarter and replaced. However, during this time there are many spots which the plastic breaks down due to consistent activity over a spot. This requires the flooring to be scraped, stripped, etc. Thanks Lacy Normington UW Health _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=2t7_gY_QgCsQnEhIbW0hGqXohzrIjiJRpXu4w5m7YEQ&m=hWEl-W67C0HeYK26kkJwaxwYz25-7T9gHERINBtJ8wo&s=xJ1cq8UebwyPgqtjwY7XKdG0q7IfyadzT9FjxdFswX8&e= ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 219, Issue 8 **************************************** From jaylundgren at gmail.com Thu Feb 10 12:38:31 2022 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 10 Feb 2022 12:38:31 -0600 Subject: [Histonet] paraffin on floors In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8023419F753@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8023419F753@HRHEX02-HOS.holyredeemer.local> Message-ID: These work pretty well to keep wax from being spread around too much. https://www.grainger.com/product/PLASTICOVER-Floor-Protection-Mats-45MT85 Place them strategically in thresholds and high traffic areas. Unlike the blue semi-permanent ones you mentioned, which break down, when these get dirty, you just peel off the dirty layer, and there is a clean sheet underneath. 30 sheets a box. Use with the aforementioned twice monthly floor stripping. Or get the floor stripped once and tell your techs that they are responsible for keeping the floor in that condition. Make a clean floor a priority just like getting slides out. As in, you can't leave for the day until your area is scraped and swept. Depends on whether or not you want to pay histotechs to scrape floors. Maybe assign it to lab aides? It only takes a few minutes a day if you start with a clean floor. Personally, I hate working in a dirty lab. From jaylundgren at gmail.com Thu Feb 10 12:54:04 2022 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 10 Feb 2022 12:54:04 -0600 Subject: [Histonet] Tiny folds in colon bx In-Reply-To: References: Message-ID: If you're not, use actual ice to cool and hydrate, works better than cold plate, etc. Cool and soak the heck out of your bxs, like, rough cut them and go to breakfast. Seriously, like 30 minutes on ice, and they'll cut like butter. Try raising the temperature of your water bath 2-3 degrees. Also, try to cut nice long ribbons. Sections from the middle of a nice, long ribbons of 12-15 sections will have the weight of the rest of the ribbon to provide tension as the ribbon is laid on the water bath. Avoids microscopic wrinkles. If you are just throwing 4-5 sections on the waterbath, they can never be as perfect as sections from the middle of a longer ribbon. I was told in training at AFIP that sections shouldn't even be taken from a ribbon that was less than 5 sections long. Obviously there are exceptions. From ASelf at tidelandshealth.org Fri Feb 11 09:49:27 2022 From: ASelf at tidelandshealth.org (Amy Self) Date: Fri, 11 Feb 2022 15:49:27 +0000 Subject: [Histonet] Telepathology Message-ID: Good Morning and Happy Friday ? I am looking to see if anyone has any information or recommended websites they could share with me on Telepathology as far as getting things set up and running. Looking for validation work-up, policies and training/competency checklist. Please forward any information to my personal email at histoamy at yahoo.com. Thanks in advance and I hope that everyone has a fantabulous weekend and stay safe. Thanks, Amy Self Senior Histology Technologist Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 Office: (843) 520-8711 aself at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From carl.hobbs at kcl.ac.uk Fri Feb 11 13:58:13 2022 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 11 Feb 2022 19:58:13 +0000 Subject: [Histonet] Tiny folds in colon bx Message-ID: Imho....your waterbath is not hot enough The sections are not expanding Sure, if you are floating sections directly onto water bath....baaad Place your ribbon on a slide that has 20% alcohol on it Float that onto your waterbath The alcohol teases the sections nicely so....no creases. NB: Leave in the waterbath until you see no creases Works for me every time NB: I do many species of gut and have no problems if.....I do the above Good luck Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 From greg.dobbin at gmail.com Tue Feb 15 10:10:01 2022 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Tue, 15 Feb 2022 12:10:01 -0400 Subject: [Histonet] Cold ischemic times Message-ID: This is more of a survey than a question: For those of you tracking and documenting your cold ischemic times for breast tissue (ie time out of body to time sliced [as needed] and immersed in formalin), and I assume most of you are...*what is your average time?* *Background:* *I ask because my director was looking for suggestions for quality indicators to report and while I feel like our cold ischemic average time is impressive at ~17 mins, she says that is pretty standard for everyone.* Thanks, Greg -- *Greg Dobbin* Chief Technologist - Anatomic Pathology Queen Elizabeth Hospital Charlottetown, PE Canada C1A 8T5 Ph: 902-894-2337 *Everything in moderation...even moderation itself**!* From ewj at pigs.ag Tue Feb 15 11:37:07 2022 From: ewj at pigs.ag (E. Wayne Johnson) Date: Wed, 16 Feb 2022 01:37:07 +0800 Subject: [Histonet] Cold ischemic times In-Reply-To: References: Message-ID: <6448fbeb-59c7-0b54-3d69-9d77ee0c5172@pigs.ag> When I first saw the title of your email I thought it was a publication OR a commentary on The Era. New York Times Cold Ischemic Times Global Times Los Angeles Times. * It was the worst of times it was the best of times Man, these are such cold ischemic times, (Brother, can you paradigm?) We never knew what time it was but we knew how sublime it was. E. Wayne Johnson DVM Enable Ag Tech Beijing. Greg Dobbin via Histonet wrote: > This is more of a survey than a question: > > For those of you tracking and documenting your cold ischemic times for > breast tissue (ie time out of body to time sliced [as needed] and immersed > in formalin), and I assume most of you are...*what is your average time?* > > *Background:* > *I ask because my director was looking for suggestions for quality > indicators to report and while I feel like our cold ischemic average time > is impressive at ~17 mins, she says that is pretty standard for everyone.* > > Thanks, > Greg > From tbraud at holyredeemer.com Tue Feb 15 12:23:47 2022 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 15 Feb 2022 18:23:47 +0000 Subject: [Histonet] cold ischemic time Message-ID: <48E053DDF6CE074DB6A7414BA05403F8023419FF12@HRHEX02-HOS.holyredeemer.local> We average within 1 minute for needle core and small biopsies, within 15 for lumpectomy or mastectomy. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, February 15, 2022 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 219, Issue 11 Today's Topics: 1. Cold ischemic times (Greg Dobbin) 2. Re: Cold ischemic times (E. Wayne Johnson) ---------------------------------------------------------------------- Message: 1 Date: Tue, 15 Feb 2022 12:10:01 -0400 From: Greg Dobbin To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cold ischemic times This is more of a survey than a question: For those of you tracking and documenting your cold ischemic times for breast tissue (ie time out of body to time sliced [as needed] and immersed in formalin), and I assume most of you are...*what is your average time?* *Background:* *I ask because my director was looking for suggestions for quality indicators to report and while I feel like our cold ischemic average time is impressive at ~17 mins, she says that is pretty standard for everyone.* Thanks, Greg *Greg Dobbin* Chief Technologist - Anatomic Pathology Queen Elizabeth Hospital Charlottetown, PE Canada C1A 8T5 Ph: 902-894-2337 *Everything in moderation...even moderation itself**!* From jennifer.koblinski at vcuhealth.org Wed Feb 16 17:01:14 2022 From: jennifer.koblinski at vcuhealth.org (Jennifer Koblinski) Date: Wed, 16 Feb 2022 23:01:14 +0000 Subject: [Histonet] job opportunity- VCU Richmond, VA Message-ID: Dear all- I am co-director of Virginia Commonwealth University Tissue Data Acquisition and Analysis Core. Unfortunately, our outstanding research technician that does our grossing, formalin-fixed paraffin processing, paraffin embedding, paraffin sectioning and automated H&E staining is retiring. We are looking to fill this position ASAP. Please see the following job positing if you are interested. We are a great group of people to work with! https://vcu.csod.com/ux/ats/careersite/1/home/requisition/486?c=vcu Best wishes, Jennifer Jennifer Koblinski, PhD (Pronouns: She/Her/Hers) Assistant Professor Director, Cancer Mouse Models Core CoDirector, Tissue and Data Acquistion and Analysis Core Virginia Commonwealth University School of Medicine Department of Pathology Massey Cancer Center 1101 E. Marshall St Sanger 4-013 Box 980662 Richmond, VA 23298 https://massey.vcu.edu/research/cores/cmmc/ https://pathology.vcu.edu/research/tissue-and-data-acquisition/tdaac-services/ From relia1 at earthlink.net Thu Feb 17 10:14:30 2022 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 17 Feb 2022 11:14:30 -0500 Subject: [Histonet] Happy Belated Valentines, Galentines and Palentines Day! Here are some sweets for the sweet! Message-ID: <002001d82419$74ad0cb0$5e072610$@earthlink.net> Hi Histopeeps, I hope this is an especially sweet week since Monday was Valentines' Day. Let me wish you a belated: Happy Valentines' Day!!! Happy "Gal"entine's Day And Happy "Pal"entine's Day!! I also have some great histology opportunities to tell you about. Please feel free to take a second and peruse the list kind of the way one peruses an Assortment of chocolates in a heart shaped box! My personal favorites are Chocolate Covered Cherries!! (Especially the ones that are 50% for the rest of the week! LOL) How about you? All of these are permanent full time positions and our clients offer excellent compensation, benefits, relocation and or sign on bonuses. Histopeeps, Here is a list of my current openings!! Let me know if anything looks good!! Management and Specialty Opportunities: * Histology Supervisor St. Louis, MO * Histology Supervisor Ft. Myers, FL * Histology Supervisor San Diego, CA * Histology Educator Aliso Viejo, CA HT/HTL Opportunities: * Salt Lake City, UT * Maryville, TN * Port Charlotte, FL * Fort Myers, FL * Abilene, TX * Irvine, CA * Detroit, MI * San Diego, CA * Aliso Viejo, CA And I have new positions coming in every day so if you don't see something you like drop me a line and let me know what you ARE looking for. I would love to help. If you are interested in any of these positions please call or text me on my cell at 407-353-5070 or toll free at 866-607-3542 or e-mail me at relia1 at earthlink.net * If you would like you can e-mail me your resume and a number where I can reach you at a time that is convenient for you. * If you are interested in looking into new job opportunities in other areas that are not mentioned above please contact me as well. * I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember... It never hurts to keep an eye open EVEN IF you are happy in your present job. I hope you had a Wonderful Valentine's Day!! Or Galentine's Day or Palentine's Day!! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions From criley at udel.edu Tue Feb 22 11:34:46 2022 From: criley at udel.edu (Charles Riley) Date: Tue, 22 Feb 2022 12:34:46 -0500 Subject: [Histonet] Sakura 4070 coverslipper troubleshooting Message-ID: Can anyone give me suggestions on how to fix an E18 error code? The machine initiates with no issues and homes itself with no problems. When I load a rack in to begin coverslipping the base moves to the far back position and then I get the error code as the basin won't move to the front position. However once I hit the stop button to abort it moves to the front on its own with no issues. Any guidance or suggestions would be helpful. Trying to get it up and running by the end of the day if possible From Johanna.Haddock at propath.com Tue Feb 22 13:19:28 2022 From: Johanna.Haddock at propath.com (Johanna Haddock) Date: Tue, 22 Feb 2022 19:19:28 +0000 Subject: [Histonet] FW: Hood - manufacturer booklet Message-ID: Hi, Does any of you have the manufacturer booklet of the hood listed below that you can share with me? ThermoFisher Scientific Gross Lab Junior 9600 series Part No. 96003 S/N 9600913639 Johanna Haddock BS, MT(ASCP) Quality System Manager/Safety Officer 1355 River Bend Dr., Dallas, TX 75247 Ph: (214) 237-1623 / Fax: (214) 237-1731 E-MAIL CONFIDENTIALITY NOTICE: The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. -----Original Message----- From: Terri Braud via Histonet Sent: Tuesday, February 15, 2022 12:24 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] cold ischemic time [CAUTION - EXTERNAL EMAIL: Do not click any links or open any attachments unless you trust the sender and know the content is safe.] We average within 1 minute for needle core and small biopsies, within 15 for lumpectomy or mastectomy. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 Honesty AccouNtability AgiLity CoLlaboration CoMpassion -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, February 15, 2022 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 219, Issue 11 Today's Topics: 1. Cold ischemic times (Greg Dobbin) 2. Re: Cold ischemic times (E. Wayne Johnson) ---------------------------------------------------------------------- Message: 1 Date: Tue, 15 Feb 2022 12:10:01 -0400 From: Greg Dobbin To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cold ischemic times This is more of a survey than a question: For those of you tracking and documenting your cold ischemic times for breast tissue (ie time out of body to time sliced [as needed] and immersed in formalin), and I assume most of you are...*what is your average time?* *Background:* *I ask because my director was looking for suggestions for quality indicators to report and while I feel like our cold ischemic average time is impressive at ~17 mins, she says that is pretty standard for everyone.* Thanks, Greg *Greg Dobbin* Chief Technologist - Anatomic Pathology Queen Elizabeth Hospital Charlottetown, PE Canada C1A 8T5 Ph: 902-894-2337 *Everything in moderation...even moderation itself**!* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cjohanna.haddock%40propath.com%7C1d8e98d40dbe42f0bf7908d9f0b23d62%7Ceab7e4b5d8f8463b8a4ac63f87390803%7C1%7C0%7C637805470589696855%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=m3FxpVdU4lko9gWdvN18uN1c8076PjxwtKVxOQsBp84%3D&reserved=0 From Branka.BruknerDabovic at nyulangone.org Tue Feb 22 16:01:41 2022 From: Branka.BruknerDabovic at nyulangone.org (Brukner Dabovic, Branka) Date: Tue, 22 Feb 2022 22:01:41 +0000 Subject: [Histonet] RBC & IHC Message-ID: <5C9E2C9A-7852-411B-8231-241E369307DB@contoso.com> Hi, Does anyone know how to reduce non-specific staining of RBC on HRP-DAB IHC slides? It happens only with some antibodies and we always treat the slides with peroxide before the staining. Branka Branka Brukner Dabovic, PhD DART Experimental Pathology Research Lab NYU Langone Health New York, NY ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= From criley at udel.edu Mon Feb 28 13:25:30 2022 From: criley at udel.edu (Charles Riley) Date: Mon, 28 Feb 2022 14:25:30 -0500 Subject: [Histonet] IHC staining of cartilage Message-ID: I recently tried running an automated ihc test on a few pieces of cartilage tissue. All of the sections fell off my slides. I am using the TOMO charged slides and have never had any issues. Do cartilage sections require longer drying times in order for the tissue to adhere better?