From amurvosh at advancederm.net Tue Aug 2 10:23:37 2022 From: amurvosh at advancederm.net (Anne Murvosh) Date: Tue, 2 Aug 2022 15:23:37 +0000 Subject: [Histonet] Small processors Message-ID: We are looking forward to ordering a new or used processor. We have the Sakura VIP5 however, I was wondering if there were smaller processors out there. We only do a max of about 60 skin specimens and the VIP is a bit large for that quantity. I would say a max of 100 specimens for future growth. Any thoughts. Thanks Anne Murvosh HT HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return or destruction. From robrankin at rankinbiomed.com Tue Aug 2 23:50:00 2022 From: robrankin at rankinbiomed.com (Rob Rankin) Date: Wed, 3 Aug 2022 00:50:00 -0400 Subject: [Histonet] Histonet Digest, Vol 225, Issue 1 In-Reply-To: References: Message-ID: I will request to cancel this Histonet Digest, if you are receiving them. Respectfully, *ROB RANKIN* | FOUNDER ROB at RANKINBIOMED.COM ? DIRECT: (248) 708-0012 <+2482155390> 14515 Mackey Rd, Holly, MI 48442 Equipment New | Equipment Refurbished | Consumables | Customer Support | Parts | Customer Reviews On Tue, Aug 2, 2022 at 1:12 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Small processors (Anne Murvosh) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 2 Aug 2022 15:23:37 +0000 > From: Anne Murvosh > To: "histonet (histonet at lists.utsouthwestern.edu)" > > Subject: [Histonet] Small processors > Message-ID: > < > MW4PR15MB4561A69B18BB95A52345398DC69D9 at MW4PR15MB4561.namprd15.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > We are looking forward to ordering a new or used processor. We have the > Sakura VIP5 however, I was wondering if there were smaller processors out > there. We only do a max of about 60 skin specimens and the VIP is a bit > large for that quantity. I would say a max of 100 specimens for future > growth. Any thoughts. Thanks Anne Murvosh HT > HIPAA Confidentiality Notice: The information and documents accompanying > this e-mail may contain confidential information that is legally privileged > and protected by federal and state law. The information is intended for use > only by the entity or individual to whom it is addressed, the authorized > recipient. The authorized recipient is obligated to maintain the > information in a safe, secure, and confidential manner. The authorized > recipient is prohibited from using the information for purposes other than > intended, prohibited from disclosing the information to any other party > unless required to do so by law or regulation, and is required to destroy > the information after its stated need has been fulfilled. If you are in > possession of this information, and are not the intended recipient, you are > hereby notified that any improper disclosure, copying, or distribution of > the information is strictly prohibited. Please notify the owner of the > information immediately and arrange for its return or destruction. > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 225, Issue 1 > **************************************** > From jmbrooksokstate at gmail.com Wed Aug 3 12:26:08 2022 From: jmbrooksokstate at gmail.com (Joseph Brooks) Date: Wed, 3 Aug 2022 11:26:08 -0600 Subject: [Histonet] Histonet Digest, Vol 225, Issue 1 In-Reply-To: References: Message-ID: Hello Anne, Have you looked at any of the Milestone Medical processors? I have used their benchtop processors in the past and they are great. Regards, Matt On Tue, Aug 2, 2022 at 11:11 AM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Small processors (Anne Murvosh) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 2 Aug 2022 15:23:37 +0000 > From: Anne Murvosh > To: "histonet (histonet at lists.utsouthwestern.edu)" > > Subject: [Histonet] Small processors > Message-ID: > < > MW4PR15MB4561A69B18BB95A52345398DC69D9 at MW4PR15MB4561.namprd15.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > We are looking forward to ordering a new or used processor. We have the > Sakura VIP5 however, I was wondering if there were smaller processors out > there. We only do a max of about 60 skin specimens and the VIP is a bit > large for that quantity. I would say a max of 100 specimens for future > growth. Any thoughts. Thanks Anne Murvosh HT > HIPAA Confidentiality Notice: The information and documents accompanying > this e-mail may contain confidential information that is legally privileged > and protected by federal and state law. The information is intended for use > only by the entity or individual to whom it is addressed, the authorized > recipient. The authorized recipient is obligated to maintain the > information in a safe, secure, and confidential manner. The authorized > recipient is prohibited from using the information for purposes other than > intended, prohibited from disclosing the information to any other party > unless required to do so by law or regulation, and is required to destroy > the information after its stated need has been fulfilled. If you are in > possession of this information, and are not the intended recipient, you are > hereby notified that any improper disclosure, copying, or distribution of > the information is strictly prohibited. Please notify the owner of the > information immediately and arrange for its return or destruction. > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 225, Issue 1 > **************************************** > From cforster at umn.edu Thu Aug 4 17:15:11 2022 From: cforster at umn.edu (Colleen Forster) Date: Thu, 4 Aug 2022 17:15:11 -0500 Subject: [Histonet] slide printers Message-ID: HEllo Histonetters, We are a research lab that has been using the Leica IPC/IPS printed for many years. Today the flash motor went out in the IPS. We are now looking into updating the slide printer. We are research only and NOT hooked into any LIS system. It would be a stand alone process. Can some of you share what you have and the pros/cons? I need to start the search for the replacement I would like. Thank you in advance. -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From tbraud at holyredeemer.com Fri Aug 5 14:03:56 2022 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 5 Aug 2022 19:03:56 +0000 Subject: [Histonet] Slide Printer Message-ID: We've been using our Leica IPS and IPC printers for over 15 years, first as stand-alone units, and now, integrated into CoPath. What a workhorse these instruments this have been! Their modern equivalent is almost identical, so parts are readily available. My recommendation would be to repair or replace with the same. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Friday, August 5, 2022 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 225, Issue 4 CAUTION: This email originated from outside Redeemer Health. Do not click links or open attachments unless you recognize the sender and know the content is safe. Contact our IT Support Center at 215-938-3900 with questions. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. slide printers (Colleen Forster) ---------------------------------------------------------------------- Message: 1 Date: Thu, 4 Aug 2022 17:15:11 -0500 From: Colleen Forster To: histonet-request Subject: [Histonet] slide printers Message-ID: Content-Type: text/plain; charset="UTF-8" HEllo Histonetters, We are a research lab that has been using the Leica IPC/IPS printed for many years. Today the flash motor went out in the IPS. We are now looking into updating the slide printer. We are research only and NOT hooked into any LIS system. It would be a stand alone process. Can some of you share what you have and the pros/cons? I need to start the search for the replacement I would like. Thank you in advance. -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 225, Issue 4 **************************************** From bcooper at chla.usc.edu Fri Aug 5 15:00:01 2022 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Fri, 5 Aug 2022 20:00:01 +0000 Subject: [Histonet] Slide Printer In-Reply-To: References: Message-ID: Similar story here, except we went with SlideMates. Before AB&T LIS integration, we were able to have CoPath print a barcode on our cassettes (Leica IPC cassette printer). The SlideMate Field Service Reps were able to translate that barcode info on the slide printers (which were still standalone at that point). That barcode on the cassettes translated to a patient name and accession number on each slide. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu -----Original Message----- From: Terri Braud via Histonet Sent: Friday, August 5, 2022 12:04 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Slide Printer (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. We've been using our Leica IPS and IPC printers for over 15 years, first as stand-alone units, and now, integrated into CoPath. What a workhorse these instruments this have been! Their modern equivalent is almost identical, so parts are readily available. My recommendation would be to repair or replace with the same. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Friday, August 5, 2022 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 225, Issue 4 CAUTION: This email originated from outside Redeemer Health. Do not click links or open attachments unless you recognize the sender and know the content is safe. Contact our IT Support Center at 215-938-3900 with questions. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://secure-web.cisco.com/1F1KV_fZiex-EE6KRGTKG8qxzUJkERovSHEyqtL7ewOuQw9H20h9v0cnDLmIbsPeAXEVKgrfixq0lEZVTa5F5rexX-Jl8Z6bpkCrBcBIdttsZepxqO6EEv7istC-HSWnsFZxEcsAU9395WISqpYJH-KAJXBTMGT9psOn0jZazN5BLIikJMYDv_VoQRKVGEoX2XSFKpkXI55DT2iSLJdwJXU5MyMnHCEqZcJrMBzuWnSOFX6zQ5FyaGiuDHRWo7ZOVog6FTvPwREuRy1SkPXgIRd3jZmihEjXKX1kHV3hrLhi5BWK_KNQ11gnXRjXbebVcP3o1ENBgvMR-_1_JeIXmo41nDm-3sbAqoo7sEVQMuhIfhyM6Yk_f3Qc4fAuuv_aFDlXTvalVC3A3vvocnlCyMNzLUAdraEIpObE_1h5T8vW0H4TDzdTZTTIysSa1lbpK7rueOPTRv7KpQ0PJSdOntg/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. slide printers (Colleen Forster) ---------------------------------------------------------------------- Message: 1 Date: Thu, 4 Aug 2022 17:15:11 -0500 From: Colleen Forster To: histonet-request Subject: [Histonet] slide printers Message-ID: Content-Type: text/plain; charset="UTF-8" HEllo Histonetters, We are a research lab that has been using the Leica IPC/IPS printed for many years. Today the flash motor went out in the IPS. We are now looking into updating the slide printer. We are research only and NOT hooked into any LIS system. It would be a stand alone process. Can some of you share what you have and the pros/cons? I need to start the search for the replacement I would like. Thank you in advance. -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1F1KV_fZiex-EE6KRGTKG8qxzUJkERovSHEyqtL7ewOuQw9H20h9v0cnDLmIbsPeAXEVKgrfixq0lEZVTa5F5rexX-Jl8Z6bpkCrBcBIdttsZepxqO6EEv7istC-HSWnsFZxEcsAU9395WISqpYJH-KAJXBTMGT9psOn0jZazN5BLIikJMYDv_VoQRKVGEoX2XSFKpkXI55DT2iSLJdwJXU5MyMnHCEqZcJrMBzuWnSOFX6zQ5FyaGiuDHRWo7ZOVog6FTvPwREuRy1SkPXgIRd3jZmihEjXKX1kHV3hrLhi5BWK_KNQ11gnXRjXbebVcP3o1ENBgvMR-_1_JeIXmo41nDm-3sbAqoo7sEVQMuhIfhyM6Yk_f3Qc4fAuuv_aFDlXTvalVC3A3vvocnlCyMNzLUAdraEIpObE_1h5T8vW0H4TDzdTZTTIysSa1lbpK7rueOPTRv7KpQ0PJSdOntg/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet ------------------------------ End of Histonet Digest, Vol 225, Issue 4 **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1F1KV_fZiex-EE6KRGTKG8qxzUJkERovSHEyqtL7ewOuQw9H20h9v0cnDLmIbsPeAXEVKgrfixq0lEZVTa5F5rexX-Jl8Z6bpkCrBcBIdttsZepxqO6EEv7istC-HSWnsFZxEcsAU9395WISqpYJH-KAJXBTMGT9psOn0jZazN5BLIikJMYDv_VoQRKVGEoX2XSFKpkXI55DT2iSLJdwJXU5MyMnHCEqZcJrMBzuWnSOFX6zQ5FyaGiuDHRWo7ZOVog6FTvPwREuRy1SkPXgIRd3jZmihEjXKX1kHV3hrLhi5BWK_KNQ11gnXRjXbebVcP3o1ENBgvMR-_1_JeIXmo41nDm-3sbAqoo7sEVQMuhIfhyM6Yk_f3Qc4fAuuv_aFDlXTvalVC3A3vvocnlCyMNzLUAdraEIpObE_1h5T8vW0H4TDzdTZTTIysSa1lbpK7rueOPTRv7KpQ0PJSdOntg/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From john at imebinc.com Sat Aug 6 12:20:49 2022 From: john at imebinc.com (=?utf-8?Q?John_O=E2=80=99Brien?=) Date: Sat, 6 Aug 2022 10:20:49 -0700 Subject: [Histonet] Histonet Digest, Vol 225, Issue 5 In-Reply-To: References: Message-ID: To whom it concerns Please contact IMEB Inc for info on refurbished Leica Slide or cassette printer we been selling refurbishing Leica slide and cassette printers for 15 years, As long as u have software and stand alone unit I have refurbished printers with complete warranty of 9 months On this unit You can use your current software to drive a refurbished slide or cassette Leica printer. Regards IMEB Inc > On Aug 6, 2022, at 10:13 AM, histonet-request at lists.utsouthwestern.edu wrote: > > ?Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Slide Printer (Terri Braud) > 2. Re: Slide Printer (Cooper, Brian) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 5 Aug 2022 19:03:56 +0000 > From: "Terri Braud" > To: "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Slide Printer > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > We've been using our Leica IPS and IPC printers for over 15 years, first as stand-alone units, and now, integrated into CoPath. What a workhorse these instruments this have been! Their modern equivalent is almost identical, so parts are readily available. My recommendation would be to repair or replace with the same. > > Terri L. Braud, HT(ASCP) > HNL Laboratories for > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3689 > Fax: 215-938-3874 > ????????? Honesty > AccouNtability > ??? AgiLity > ??? CoLlaboration > ? CoMpassion > > -----Original Message----- > From: histonet-request at lists.utsouthwestern.edu > Sent: Friday, August 5, 2022 1:00 PM > To: histonet at lists.utsouthwestern.edu > Subject: [EXTERNAL] Histonet Digest, Vol 225, Issue 4 > > CAUTION: This email originated from outside Redeemer Health. Do not click links or open attachments unless you recognize the sender and know the content is safe. Contact our IT Support Center at 215-938-3900 with questions. > > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. slide printers (Colleen Forster) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 4 Aug 2022 17:15:11 -0500 > From: Colleen Forster > To: histonet-request > Subject: [Histonet] slide printers > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > HEllo Histonetters, > > We are a research lab that has been using the Leica IPC/IPS printed for many years. Today the flash motor went out in the IPS. We are now looking into updating the slide printer. > > We are research only and NOT hooked into any LIS system. It would be a stand alone process. Can some of you share what you have and the pros/cons? > I need to start the search for the replacement I would like. > > Thank you in advance. > > -- > Colleen Forster HT(ASCP)QIHC > BLS Histology and IHC Laboratory > Jackson Hall, Room 2-155 > 321 Church St. SE > Minneapolis, MN 55455 > 612-626-1930 > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 225, Issue 4 > **************************************** > > > > > ------------------------------ > > Message: 2 > Date: Fri, 5 Aug 2022 20:00:01 +0000 > From: "Cooper, Brian" > To: Terri Braud , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Slide Printer > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Similar story here, except we went with SlideMates. Before AB&T LIS integration, we were able to have CoPath print a barcode on our cassettes (Leica IPC cassette printer). The SlideMate Field Service Reps were able to translate that barcode info on the slide printers (which were still standalone at that point). That barcode on the cassettes translated to a patient name and accession number on each slide. > > Thanks, > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 > bcooper at chla.usc.edu > > -----Original Message----- > From: Terri Braud via Histonet > Sent: Friday, August 5, 2022 12:04 PM > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Slide Printer (EXTERNAL EMAIL) > > ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. > > We've been using our Leica IPS and IPC printers for over 15 years, first as stand-alone units, and now, integrated into CoPath. What a workhorse these instruments this have been! Their modern equivalent is almost identical, so parts are readily available. My recommendation would be to repair or replace with the same. > > Terri L. Braud, HT(ASCP) > HNL Laboratories for > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3689 > Fax: 215-938-3874 > ????????? Honesty > AccouNtability > ??? AgiLity > ??? CoLlaboration > ? CoMpassion > > -----Original Message----- > From: histonet-request at lists.utsouthwestern.edu > Sent: Friday, August 5, 2022 1:00 PM > To: histonet at lists.utsouthwestern.edu > Subject: [EXTERNAL] Histonet Digest, Vol 225, Issue 4 > > CAUTION: This email originated from outside Redeemer Health. Do not click links or open attachments unless you recognize the sender and know the content is safe. Contact our IT Support Center at 215-938-3900 with questions. > > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://secure-web.cisco.com/1F1KV_fZiex-EE6KRGTKG8qxzUJkERovSHEyqtL7ewOuQw9H20h9v0cnDLmIbsPeAXEVKgrfixq0lEZVTa5F5rexX-Jl8Z6bpkCrBcBIdttsZepxqO6EEv7istC-HSWnsFZxEcsAU9395WISqpYJH-KAJXBTMGT9psOn0jZazN5BLIikJMYDv_VoQRKVGEoX2XSFKpkXI55DT2iSLJdwJXU5MyMnHCEqZcJrMBzuWnSOFX6zQ5FyaGiuDHRWo7ZOVog6FTvPwREuRy1SkPXgIRd3jZmihEjXKX1kHV3hrLhi5BWK_KNQ11gnXRjXbebVcP3o1ENBgvMR-_1_JeIXmo41nDm-3sbAqoo7sEVQMuhIfhyM6Yk_f3Qc4fAuuv_aFDlXTvalVC3A3vvocnlCyMNzLUAdraEIpObE_1h5T8vW0H4TDzdTZTTIysSa1lbpK7rueOPTRv7KpQ0PJSdOntg/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. slide printers (Colleen Forster) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 4 Aug 2022 17:15:11 -0500 > From: Colleen Forster > To: histonet-request > Subject: [Histonet] slide printers > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > HEllo Histonetters, > > We are a research lab that has been using the Leica IPC/IPS printed for many years. Today the flash motor went out in the IPS. We are now looking into updating the slide printer. > > We are research only and NOT hooked into any LIS system. It would be a stand alone process. Can some of you share what you have and the pros/cons? > I need to start the search for the replacement I would like. > > Thank you in advance. > > -- > Colleen Forster HT(ASCP)QIHC > BLS Histology and IHC Laboratory > Jackson Hall, Room 2-155 > 321 Church St. SE > Minneapolis, MN 55455 > 612-626-1930 > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://secure-web.cisco.com/1F1KV_fZiex-EE6KRGTKG8qxzUJkERovSHEyqtL7ewOuQw9H20h9v0cnDLmIbsPeAXEVKgrfixq0lEZVTa5F5rexX-Jl8Z6bpkCrBcBIdttsZepxqO6EEv7istC-HSWnsFZxEcsAU9395WISqpYJH-KAJXBTMGT9psOn0jZazN5BLIikJMYDv_VoQRKVGEoX2XSFKpkXI55DT2iSLJdwJXU5MyMnHCEqZcJrMBzuWnSOFX6zQ5FyaGiuDHRWo7ZOVog6FTvPwREuRy1SkPXgIRd3jZmihEjXKX1kHV3hrLhi5BWK_KNQ11gnXRjXbebVcP3o1ENBgvMR-_1_JeIXmo41nDm-3sbAqoo7sEVQMuhIfhyM6Yk_f3Qc4fAuuv_aFDlXTvalVC3A3vvocnlCyMNzLUAdraEIpObE_1h5T8vW0H4TDzdTZTTIysSa1lbpK7rueOPTRv7KpQ0PJSdOntg/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet > > ------------------------------ > > End of Histonet Digest, Vol 225, Issue 4 > **************************************** > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://secure-web.cisco.com/1F1KV_fZiex-EE6KRGTKG8qxzUJkERovSHEyqtL7ewOuQw9H20h9v0cnDLmIbsPeAXEVKgrfixq0lEZVTa5F5rexX-Jl8Z6bpkCrBcBIdttsZepxqO6EEv7istC-HSWnsFZxEcsAU9395WISqpYJH-KAJXBTMGT9psOn0jZazN5BLIikJMYDv_VoQRKVGEoX2XSFKpkXI55DT2iSLJdwJXU5MyMnHCEqZcJrMBzuWnSOFX6zQ5FyaGiuDHRWo7ZOVog6FTvPwREuRy1SkPXgIRd3jZmihEjXKX1kHV3hrLhi5BWK_KNQ11gnXRjXbebVcP3o1ENBgvMR-_1_JeIXmo41nDm-3sbAqoo7sEVQMuhIfhyM6Yk_f3Qc4fAuuv_aFDlXTvalVC3A3vvocnlCyMNzLUAdraEIpObE_1h5T8vW0H4TDzdTZTTIysSa1lbpK7rueOPTRv7KpQ0PJSdOntg/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 225, Issue 5 > **************************************** From carlos.torresvega at gmail.com Sat Aug 6 13:36:55 2022 From: carlos.torresvega at gmail.com (Carlos Torres Vega) Date: Sat, 6 Aug 2022 13:36:55 -0500 Subject: [Histonet] Asking for software Message-ID: <627704CA-FEE5-4FD1-BDB8-3B9BA0A5DA71@gmail.com> Terry Braud You have used a Leica ISP slide printer as stand alone unit, what software did you use. ? Because I have one but no software Enviado desde mi iPhone From Donna.Willis at BSWHealth.org Sat Aug 6 14:29:32 2022 From: Donna.Willis at BSWHealth.org (Willis, Donna G) Date: Sat, 6 Aug 2022 19:29:32 +0000 Subject: [Histonet] Asking for software In-Reply-To: <627704CA-FEE5-4FD1-BDB8-3B9BA0A5DA71@gmail.com> References: <627704CA-FEE5-4FD1-BDB8-3B9BA0A5DA71@gmail.com> Message-ID: Software comes with the unit when you purchase it from Leica. Donna Willis Anatomic Pathology Manager Baylor University Medical Center Baylor Scott&White Health 214-820-2465 office ________________________________ From: Carlos Torres Vega via Histonet Sent: Saturday, August 6, 2022 1:36:55 PM To: histonet at lists.utsouthwestern.edu Subject: {EXTERNAL} [Histonet] Asking for software CAUTION: This email originated outside of BSWH. The actual sender is (histonet-bounces at lists.utsouthwestern.edu) which may be different from the display address in the From: field. Avoid action unless you know the content is safe. Report suspicious emails using the PhishAlarm button located in your Outlook ribbon. Terry Braud You have used a Leica ISP slide printer as stand alone unit, what software did you use. ? Because I have one but no software Enviado desde mi iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!B2MyJx_RiLO4q4tAgv3NdR2bTxnBePAyENrAlgr7I-omug3pBifrdzsNMGN_aClD0Vp5SXXWzYO-RKREHx334_BTsBh3bz-0$ ********************************************************************** The information contained in this e-mail may be privileged, confidential, and/or protected from disclosure. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly prohibited and no waiver of any attorney-client, work product, or other privilege is intended. No binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, is permitted by e-mail without express written confirmation by a duly authorized representative of Baylor Scott & White Health. From cforster at umn.edu Sat Aug 6 14:43:35 2022 From: cforster at umn.edu (Colleen Forster) Date: Sat, 6 Aug 2022 14:43:35 -0500 Subject: [Histonet] Asking for software In-Reply-To: References: <627704CA-FEE5-4FD1-BDB8-3B9BA0A5DA71@gmail.com> Message-ID: Donna, I know first hand, if you have any computer issues that take out your software, ( power surge for example) Leica does NOT reinstall the program. You are left to find a different program. It took us a long time to get a program so we could use both the IPC/IPS again. The new software isn't even close to the program it came with but it beats handwriting them. This person may have had a similar problem so, like us, has the units and no software to run them. ???? On Sat, Aug 6, 2022, 2:29 PM Willis, Donna G via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Software comes with the unit when you purchase it from Leica. > > Donna Willis > Anatomic Pathology Manager > Baylor University Medical Center > Baylor Scott&White Health > 214-820-2465 office > ________________________________ > From: Carlos Torres Vega via Histonet > Sent: Saturday, August 6, 2022 1:36:55 PM > To: histonet at lists.utsouthwestern.edu > Subject: {EXTERNAL} [Histonet] Asking for software > > > CAUTION: This email originated outside of BSWH. The actual sender is ( > histonet-bounces at lists.utsouthwestern.edu) which may be different from > the display address in the From: field. Avoid action unless you know the > content is safe. Report suspicious emails using the PhishAlarm button > located in your Outlook ribbon. > > Terry Braud > You have used a Leica ISP slide printer as stand alone unit, what > software did you use. ? > Because I have one but no software > > > Enviado desde mi iPhone > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!B2MyJx_RiLO4q4tAgv3NdR2bTxnBePAyENrAlgr7I-omug3pBifrdzsNMGN_aClD0Vp5SXXWzYO-RKREHx334_BTsBh3bz-0$ > > ********************************************************************** > The information contained in this e-mail may be privileged, confidential, > and/or protected from disclosure. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you are > not the intended recipient (or have received this e-mail in error) please > notify the sender immediately and destroy this e-mail. Any unauthorized > copying, disclosure or distribution of the material in this e-mail is > strictly prohibited and no waiver of any attorney-client, work product, or > other privilege is intended. No binding agreement on behalf of Baylor Scott > & White Health, or any affiliated entity, is permitted by e-mail without > express written confirmation by a duly authorized representative of Baylor > Scott & White Health. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From michael.gudo at morphisto.de Sat Aug 6 16:06:38 2022 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Sat, 6 Aug 2022 23:06:38 +0200 Subject: [Histonet] Asking for software In-Reply-To: References: <627704CA-FEE5-4FD1-BDB8-3B9BA0A5DA71@gmail.com> Message-ID: <64AB4774-0720-4602-9F54-BFBDF1558811@morphisto.de> Hello all IPS/IPS users, we used both printer, IPS and IPC just with the original printer driver and a database-application which offers us much more options than the application that comes with the printers (NiceLabel - as far as I know). So I think it should be possible to reinstall the printer driver from the CD ROM that comes with the printers and then to use the printers via Excel, Word, or any other software, best case from a database. Kind regards Michael > Am 06.08.2022 um 21:43 schrieb Colleen Forster via Histonet : > > Donna, > > I know first hand, if you have any computer issues that take out your > software, ( power surge for example) Leica does NOT reinstall the program. > You are left to find a different program. It took us a long time to get a > program so we could use both the IPC/IPS again. The new software isn't even > close to the program it came with but it beats handwriting them. > > This person may have had a similar problem so, like us, has the units and > no software to run them. ???? > > On Sat, Aug 6, 2022, 2:29 PM Willis, Donna G via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Software comes with the unit when you purchase it from Leica. >> >> Donna Willis >> Anatomic Pathology Manager >> Baylor University Medical Center >> Baylor Scott&White Health >> 214-820-2465 office >> ________________________________ >> From: Carlos Torres Vega via Histonet >> Sent: Saturday, August 6, 2022 1:36:55 PM >> To: histonet at lists.utsouthwestern.edu >> Subject: {EXTERNAL} [Histonet] Asking for software >> >> >> CAUTION: This email originated outside of BSWH. The actual sender is ( >> histonet-bounces at lists.utsouthwestern.edu) which may be different from >> the display address in the From: field. Avoid action unless you know the >> content is safe. Report suspicious emails using the PhishAlarm button >> located in your Outlook ribbon. >> >> Terry Braud >> You have used a Leica ISP slide printer as stand alone unit, what >> software did you use. ? >> Because I have one but no software >> >> >> Enviado desde mi iPhone >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> >> https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!B2MyJx_RiLO4q4tAgv3NdR2bTxnBePAyENrAlgr7I-omug3pBifrdzsNMGN_aClD0Vp5SXXWzYO-RKREHx334_BTsBh3bz-0$ >> >> ********************************************************************** >> The information contained in this e-mail may be privileged, confidential, >> and/or protected from disclosure. If you are the intended recipient, >> further disclosures are prohibited without proper authorization. If you are >> not the intended recipient (or have received this e-mail in error) please >> notify the sender immediately and destroy this e-mail. Any unauthorized >> copying, disclosure or distribution of the material in this e-mail is >> strictly prohibited and no waiver of any attorney-client, work product, or >> other privilege is intended. No binding agreement on behalf of Baylor Scott >> & White Health, or any affiliated entity, is permitted by e-mail without >> express written confirmation by a duly authorized representative of Baylor >> Scott & White Health. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************** MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Schumannstrasse 144 63069 Offenbach am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From jsimmonds at biocare.net Mon Aug 8 07:38:42 2022 From: jsimmonds at biocare.net (Jill Simmonds) Date: Mon, 8 Aug 2022 12:38:42 +0000 Subject: [Histonet] Histonet Digest, Vol 225, Issue 5--REMOVE FROM LIST In-Reply-To: References: Message-ID: Please remove me from subscription list. Thanks, Jill Jill Simmonds |?Account Executive Clinical and Research Reagents Phone:?925.586.6268 |?www.biocare.net Biocare Medical, LLC 60 Berry Drive, Pacheco CA 94553 This electronic transmission (and any attached document) contains information from Biocare Medical, LLC. They are intended only for named recipients above, and contain information that may be confidential or privileged. If you are not an intended recipient, you must not read, copy, use or disclose this communication. Please also notify the sender by replying to this message, and then delete all copies of it from your system. -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Saturday, August 6, 2022 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 225, Issue 5 CAUTION: This email originated from outside of Biocare Medical. Do not click links or open attachments unless you recognize the sender and know the content is safe. Please contact the IT Help Desk if you need assistance. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From akemiat3377 at gmail.com Tue Aug 9 16:50:48 2022 From: akemiat3377 at gmail.com (Akemi) Date: Tue, 9 Aug 2022 14:50:48 -0700 Subject: [Histonet] Absence of Crystals in stains Message-ID: <61239A7F-1975-43D9-B9F8-D26F1E9C3D6B@gmail.com> I received this from a pathologist friend and need your help. Unfortunately, I can?t attach the images he sent me on histonet. ?I?ve got a histopuzzle for you. This is a fungus ball from a maxillary sinus. On the H&E, it is loaded with these birefringent crystals, which I hypothesize are calcium oxalate. On the GMS stain, however, the crystals are completely absent. Do you think there?s some reagent in the GMS procedure that dissolves crystals that would be resistant to tissue processing and the H&E stain? ? Thanks in advance! Akemi Allison BS, HT/HTL Sent from my iPhone From gu.lang at gmx.at Wed Aug 10 04:30:03 2022 From: gu.lang at gmx.at (Gudrun Lang) Date: Wed, 10 Aug 2022 11:30:03 +0200 Subject: [Histonet] Absence of Crystals in stains In-Reply-To: <61239A7F-1975-43D9-B9F8-D26F1E9C3D6B@gmail.com> References: <61239A7F-1975-43D9-B9F8-D26F1E9C3D6B@gmail.com> Message-ID: <000f01d8ac9b$c5b85550$5128fff0$@gmx.at> My first thought about calciumcrystals is solving by the chromatic acid. I would recommend a vonKossa stain for calcium deposits. Regards Gudrun Lang -----Urspr?ngliche Nachricht----- Von: Akemi via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Dienstag, 9. August 2022 23:51 An: Histonet Betreff: [Histonet] Absence of Crystals in stains I received this from a pathologist friend and need your help. Unfortunately, I can?t attach the images he sent me on histonet. ?I?ve got a histopuzzle for you. This is a fungus ball from a maxillary sinus. On the H&E, it is loaded with these birefringent crystals, which I hypothesize are calcium oxalate. On the GMS stain, however, the crystals are completely absent. Do you think there?s some reagent in the GMS procedure that dissolves crystals that would be resistant to tissue processing and the H&E stain? ? Thanks in advance! Akemi Allison BS, HT/HTL Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Toni.Rathborne at RWJBH.org Mon Aug 15 08:45:41 2022 From: Toni.Rathborne at RWJBH.org (Rathborne, Toni) Date: Mon, 15 Aug 2022 13:45:41 +0000 Subject: [Histonet] Stainer comparisons Message-ID: Good morning all, We're at the point where we are looking at replacing our H&E stainer, and I'm wondering what opinions are out there about the Leica HistoCore Spectra ST - High Throughput Routine & Special Stainer and the Sakura Prisma Plus Special Stainer Configuration. What specials do you run on these stainers? Does this significantly increase the TAT of the H&E slides? Overall, what do you like or not like about either? Thank you, Toni ------------------------------------------------------------------------------------ NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. From rbasilio9 at sbcglobal.net Mon Aug 15 13:09:50 2022 From: rbasilio9 at sbcglobal.net (RENE MORALES) Date: Mon, 15 Aug 2022 11:09:50 -0700 Subject: [Histonet] Stainer comparisons In-Reply-To: References: Message-ID: <40CBA05C-4304-40DA-81A7-BA5901024E04@sbcglobal.net> Toni, the complete information can be found on the manufacturer specifications for these auto stainers. The only factor to be solved, no matter what instrument you will be selecting, is that you keep close monitoring of the service required for them. Important to be on the correct TAT. Other factors are influencing the good results as well, Thanks for sharing, Ren? Morales Sent from my iPhone > On Aug 15, 2022, at 07:23, Rathborne, Toni via Histonet wrote: > > ? > Good morning all, > > We're at the point where we are looking at replacing our H&E stainer, and I'm wondering what opinions are out there about the Leica HistoCore Spectra ST - High Throughput Routine & Special Stainer and the Sakura Prisma Plus Special Stainer Configuration. What specials do you run on these stainers? Does this significantly increase the TAT of the H&E slides? Overall, what do you like or not like about either? > > Thank you, > > Toni > > > ------------------------------------------------------------------------------------ > > NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Toni.Rathborne at RWJBH.org Mon Aug 15 14:01:30 2022 From: Toni.Rathborne at RWJBH.org (Rathborne, Toni) Date: Mon, 15 Aug 2022 19:01:30 +0000 Subject: [Histonet] Stainer comparisons In-Reply-To: <40CBA05C-4304-40DA-81A7-BA5901024E04@sbcglobal.net> References: <40CBA05C-4304-40DA-81A7-BA5901024E04@sbcglobal.net> Message-ID: <3f09c991b34b42a6a8ee727ee6232fe7@RWJBH.org> Rene, I have the information from the vendors as you suggested. What I'm looking for is opinions. If you (or anyone else on histonet) have experience with either or both of these stainers, I'd like to know what you feel are the best features. Also looking to see what features are not as well praised or useful. Which specials do you run, and how do they compare to what you were previously using? How did getting one of these stainers impact your day-to-day operations? Did the positives outweigh the negatives, if there were any. Thank you, Toni -----Original Message----- From: RENE MORALES Sent: Monday, August 15, 2022 2:10 PM To: Rathborne, Toni Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Stainer comparisons *** This is an External Email *** Toni, the complete information can be found on the manufacturer specifications for these auto stainers. The only factor to be solved, no matter what instrument you will be selecting, is that you keep close monitoring of the service required for them. Important to be on the correct TAT. Other factors are influencing the good results as well, Thanks for sharing, Ren? Morales Sent from my iPhone > On Aug 15, 2022, at 07:23, Rathborne, Toni via Histonet wrote: > > ? > Good morning all, > > We're at the point where we are looking at replacing our H&E stainer, and I'm wondering what opinions are out there about the Leica HistoCore Spectra ST - High Throughput Routine & Special Stainer and the Sakura Prisma Plus Special Stainer Configuration. What specials do you run on these stainers? Does this significantly increase the TAT of the H&E slides? Overall, what do you like or not like about either? > > Thank you, > > Toni > > > ------------------------------------------------------------------------------------ > > NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KU82p_qNqnQ!-1hvuN85tc17G4Eh9AR9fMAJfXtxQlF_52qRRP2hAEvASOsPKZee7BUhShCJ1qYbM8qYzVDjtu0f0eNgajxq5vVJag$ From rbasilio9 at sbcglobal.net Mon Aug 15 15:12:46 2022 From: rbasilio9 at sbcglobal.net (RENE MORALES) Date: Mon, 15 Aug 2022 13:12:46 -0700 Subject: [Histonet] Stainer comparisons In-Reply-To: <3f09c991b34b42a6a8ee727ee6232fe7@RWJBH.org> References: <3f09c991b34b42a6a8ee727ee6232fe7@RWJBH.org> Message-ID: <8F2FCC70-EDE2-44CE-A00A-3C15BDB6E425@sbcglobal.net> Toni, hope my 5 cents contribution will Help in deciding your future auto stainer. I have experience in Sakura units, 6 + years, and an old Leica unit, 10 + years. The most positive feature for the first unit is that once is giving the proper maintenance is that it works pretty well, the good advantage is that you can load in every oven, (coming with 2 oven dwells), but if you overloaded it will delay your H&E staining. I think with other models is occurring the same results? Other advantage now is that you can attach an automatic cover slipper. (Film cover slipped)? It?s important to have at least 2 units for a big number of H&E slides? I was working in a facility with at least 45 hundred H&E slides, no counting levels per protocols, recuts, etc., The only downside for all units, is that they cannot left without proper regular maintenance and service. At this moment you may have almost decided which unit serves your lab needs better. Thanks for your reply, Ren? Morales Sent from my iPhone > On Aug 15, 2022, at 12:03, Rathborne, Toni wrote: > > ?Rene, > > I have the information from the vendors as you suggested. What I'm looking for is opinions. If you (or anyone else on histonet) have experience with either or both of these stainers, I'd like to know what you feel are the best features. Also looking to see what features are not as well praised or useful. Which specials do you run, and how do they compare to what you were previously using? How did getting one of these stainers impact your day-to-day operations? Did the positives outweigh the negatives, if there were any. > > Thank you, > Toni > > -----Original Message----- > From: RENE MORALES > Sent: Monday, August 15, 2022 2:10 PM > To: Rathborne, Toni > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Stainer comparisons > > > *** This is an External Email *** > > Toni, the complete information can be found on the manufacturer specifications for these auto stainers. The only factor to be solved, no matter what instrument you will be selecting, is that you keep close monitoring of the service required for them. Important to be on the correct TAT. > Other factors are influencing the good results as well, > Thanks for sharing, > Ren? Morales > > Sent from my iPhone > >> On Aug 15, 2022, at 07:23, Rathborne, Toni via Histonet wrote: >> >> ? >> Good morning all, >> >> We're at the point where we are looking at replacing our H&E stainer, and I'm wondering what opinions are out there about the Leica HistoCore Spectra ST - High Throughput Routine & Special Stainer and the Sakura Prisma Plus Special Stainer Configuration. What specials do you run on these stainers? Does this significantly increase the TAT of the H&E slides? Overall, what do you like or not like about either? >> >> Thank you, >> >> Toni >> >> >> ------------------------------------------------------------------------------------ >> >> NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KU82p_qNqnQ!-1hvuN85tc17G4Eh9AR9fMAJfXtxQlF_52qRRP2hAEvASOsPKZee7BUhShCJ1qYbM8qYzVDjtu0f0eNgajxq5vVJag$ > From wilfong1923 at gmail.com Wed Aug 17 10:41:27 2022 From: wilfong1923 at gmail.com (John Frazier) Date: Wed, 17 Aug 2022 11:41:27 -0400 Subject: [Histonet] Stainer comparisons In-Reply-To: <40CBA05C-4304-40DA-81A7-BA5901024E04@sbcglobal.net> References: <40CBA05C-4304-40DA-81A7-BA5901024E04@sbcglobal.net> Message-ID: <29E51433-19F4-420B-B5E4-B098EF4BE6A2@gmail.com> Are used to work for Roche. The HE600 is an option that I would not choose. The instrument has too many moving parts and the rate of service is much higher than any of the dip and dunk strainers on the market. The reagents are proprietary, so you are forced to use Roche staining reagents. The Dako H&E strainer is bad too. Sent from my iPhone > On Aug 15, 2022, at 2:09 PM, RENE MORALES wrote: > > ?Toni, the complete information can be found on the manufacturer specifications for these auto stainers. The only factor to be solved, no matter what instrument you will be selecting, is that you keep close monitoring of the service required for them. Important to be on the correct TAT. > Other factors are influencing the good results as well, > Thanks for sharing, > Ren? Morales > > Sent from my iPhone > >> On Aug 15, 2022, at 07:23, Rathborne, Toni via Histonet wrote: >> >> ? >> Good morning all, >> >> We're at the point where we are looking at replacing our H&E stainer, and I'm wondering what opinions are out there about the Leica HistoCore Spectra ST - High Throughput Routine & Special Stainer and the Sakura Prisma Plus Special Stainer Configuration. What specials do you run on these stainers? Does this significantly increase the TAT of the H&E slides? Overall, what do you like or not like about either? >> >> Thank you, >> >> Toni >> >> >> ------------------------------------------------------------------------------------ >> >> NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From c.tague at pathologyarts.com Mon Aug 22 11:05:36 2022 From: c.tague at pathologyarts.com (Curt Tague) Date: Mon, 22 Aug 2022 16:05:36 +0000 Subject: [Histonet] Miami histology on the job training question Message-ID: Hi all, specifically in the Miami area... I have a friend who is interested in learning more about being a histotech... unfortunately I'm across the country and can't help train. Do any of you know of a facility who might be looking for a tech and is willing to train, from scratch, zero experience? Hospital, private lab, really anything to get the training and experience. Thanks, Curt From criley at udel.edu Wed Aug 24 13:55:18 2022 From: criley at udel.edu (Charles Riley) Date: Wed, 24 Aug 2022 14:55:18 -0400 Subject: [Histonet] Immunoflouresence staining Message-ID: I have been given an antibody that is used for flow cytometry yet the researcher wants to use it for IF staining. The protocol they provided is for the flow cytometry staining. Will this produce the immunoflourescense results they are looking for or do i need to use a different method? From cforster at umn.edu Wed Aug 24 14:16:49 2022 From: cforster at umn.edu (Colleen Forster) Date: Wed, 24 Aug 2022 14:16:49 -0500 Subject: [Histonet] Immunoflouresence staining In-Reply-To: References: Message-ID: In my experience if the antibody are designed for flow they rarely work on tissue samples. Colleen Forster HT(ASCP)QIHC University of MN On Wed, Aug 24, 2022 at 1:56 PM Charles Riley via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I have been given an antibody that is used for flow cytometry yet the > researcher wants to use it for IF staining. The protocol they provided is > for the flow cytometry staining. Will this produce the immunoflourescense > results they are looking for or do i need to use a different method? > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From jkiernan at uwo.ca Wed Aug 24 22:09:52 2022 From: jkiernan at uwo.ca (John Kiernan) Date: Thu, 25 Aug 2022 03:09:52 +0000 Subject: [Histonet] Immunofluorescense staining In-Reply-To: References: Message-ID: If the antibody's supplier does not provide instructions for IHC you will need to test a wide range of concentrations of the primary antibody on sections known to contain the antigen in easily identifiable cell-types or other sites. Use a secondary antibody and detection system that you have already found reliable for primary antibodies raised in the same species as that in which the antibody for flow cytometry was raised. This would probably be a fluorescently labelled secondary antibody if "immunoflourescense" is specified. The researcher probably needs to read a book about immunohistochemistry before expecting someone else to do it for her/him. An investment of $100 or so for a lab, along with a few hours of reading (by boss and workers), can save many hundreds of dollars that might otherwise be spent on antibodies and other reagents that don't work as expected. A good one to start with is Suvarna, S. K., Layton, C., Bancroft, J. D., eds. 2018. Bancroft's Theory and Practice of Histological Techniques 8th ed. London: Churchill Livingstone Elsevier. ISBN: 978-0-7020-6864-5. (NO, I don't have any vested interest!). I'm sure other histonetters will come up with similar and perhaps better suggestions. Beware of working from "protocols" informally passed around among technicians, grad students and research fellows without published references (papers, books) that you can check before investing time, effort and money in a technique that's new to you. Informal lab notes are often locally treated as if they "have Authority, not as the Scribes". In practical science it's probably best to go first with the Scribes, because their writings have been severely reviewed (if in good journals). Good books have references to peer-reviewed papers. I could go on and on, but that's enough of my grumpy advice for now. John Kiernan London, Canada https://www.schulich.uwo.ca/anatomy/people/faculty/emeriti/kiernan_john.html = = = ________________________________ From: Charles Riley via Histonet Sent: August 24, 2022 2:55 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Immunoflouresence staining I have been given an antibody that is used for flow cytometry yet the researcher wants to use it for IF staining. The protocol they provided is for the flow cytometry staining. Will this produce the immunoflourescense results they are looking for or do i need to use a different method? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at udel.edu Tue Aug 30 14:50:18 2022 From: criley at udel.edu (Charles Riley) Date: Tue, 30 Aug 2022 15:50:18 -0400 Subject: [Histonet] Automated ihc staining of bone Message-ID: I am working on trying to get IHC stains optimized on bone samples of rat tibias. The issue I am running into is that the periosteum is separating from the bone marrow and wrinkling up over top of the rest of the sample. If anyone has any techniques to prevent this wrinkling/detachment of tissue from the main section during automated ihc staining it would be greatly appreciated. From bcooper at chla.usc.edu Tue Aug 30 15:09:14 2022 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Tue, 30 Aug 2022 20:09:14 +0000 Subject: [Histonet] Automated ihc staining of bone In-Reply-To: References: Message-ID: <9fb4ec5cbe5a49d5b8cba5c9cef93a57@chla.usc.edu> Charles, Try and blot your sections dry before placing them into the oven. After picking up your section, place your slide onto a flat surface and take a paper towel or piece of filter paper, and gently press it down on top of the section to wick away all of the moisture. We use this technique frequently here on bone marrow cores. Thanks, Brian D. Cooper, HT (ASCP)CM QIHC| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper at chla.usc.edu -----Original Message----- From: Charles Riley via Histonet Sent: Tuesday, August 30, 2022 12:50 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Automated ihc staining of bone (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. I am working on trying to get IHC stains optimized on bone samples of rat tibias. The issue I am running into is that the periosteum is separating from the bone marrow and wrinkling up over top of the rest of the sample. If anyone has any techniques to prevent this wrinkling/detachment of tissue from the main section during automated ihc staining it would be greatly appreciated. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1srYnJjJBwOg5qJEJ_epFgbAXCjZWoirSFghf1tBQDTt4Kt86glQhWXDUFKHHe2BviKDOGHc_YOZrH3k3sKiGazjO1fRhU2GFJi18RGa2bb0f-VI2JMFwgoRCh3Mv4BFJnHqPjO4A8wLuoBUVteRZSMMDezrs0-eKxExtxe_Bn1OanJnxSFmzWIQXJl9XzUCwT1uCqSj9CFXMwW4swVLbFy3qpaq_74-LnLDO6yiQlBPyYP1upb0iwltU9iWQo-lfrXjFfKlYAHruMGUbJftFh1zBiA4sOKi90ubPDFxSvFr3-Aerb35g3yHJKIG5lZ2K0RuznvEbH-EjWmb7n3uhym4J61BoaaMBF1sH4MG7XG7yiiK5CIqm9SvxKGHyM5MeQXiTb_Ehnc74qW2NkSkEur2f06DKlv742nj6i9Ge70vNqu9tXdlIft0Q4nj4mBsO/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From alida.bailleul at gmail.com Tue Aug 30 21:27:56 2022 From: alida.bailleul at gmail.com (Alida Bailleul) Date: Wed, 31 Aug 2022 10:27:56 +0800 Subject: [Histonet] Information on the resistance/viability of different tissues/cells to different freezing temperatures? In-Reply-To: References: Message-ID: Where can I find information on the resistance of different tissues/cells (e.g., bone, cartilage) to different freezing temperatures (e.g., -4C; -24C; -70C ) ? I am asking about frozen tissues that were not cryopreserved. I think there may be a lot of experimental studies that compared the resistance of cells/tissues to freeze-thaw cycles at different temperatures (and also compared fresh frozen versus cryopreserved) but I didn't really find a lot of studies. Moreover studies don't really compare different tissue types, they focus on one type of tissue per study. Please guide me towards some papers (perhaps in the old literature? Even before cryopreservation was discovered?). I think there might be information in papers about how to store osteochondral grafts, but I did not find the information I was looking for. Thank you in advance Best wishes Alida -- Dr. Alida M. Bailleul Associate Professor Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences www.ivpp-avianevolution.com & Research Associate of Paleontology, Museum of the Rockies, Montana State University Google Scholar - ResearchGate From Charles.Bacon at baystatehealth.org Wed Aug 31 12:23:34 2022 From: Charles.Bacon at baystatehealth.org (Bacon, Charles) Date: Wed, 31 Aug 2022 17:23:34 +0000 Subject: [Histonet] Automated ihc staining of bone Message-ID: When we have section lifting during IHC, we will use a concentrated Poly-L Lysine solution. You can dilute this solution but instead we will use a direct application (wet the tip of a gloved finger with the solution, and wipe down the slide, let dry) on one of our charged slides. This may cause a degree of counter stain background but always improves the IHC result for the pathologist. We use this for bone or heavily keratinized skin sections. Supplies: Poly-L: Sigma Diagnostics? Poly-L-lysine Solution - SDP8920A Slides: TYPENEX Medical Microscope Slides - PS0101P Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786? Fax: 413-322-4790 Charles.Bacon at baystatehealth.org -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Wednesday, August 31, 2022 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 225, Issue 14 ? 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