From Nancy.Schmitt at mercyhealth.com Fri Apr 1 08:59:43 2022 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Fri, 1 Apr 2022 13:59:43 +0000 Subject: [Histonet] ultrasafe formalin dispense Message-ID: Happy Friday! Is anyone using the Ultrasafe Formalin Dispensing system? Here is a link https://www.milestonemedsrl.com/us/product/ultrasafe/ Appreciate hearing from anyone on what your thoughts are - good or bad. Thank you much, Nancy Schmitt HT, MLT(ASCP) Pathology Support Services MercyOne Dubuque Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From ricca.dario at gmail.com Fri Apr 1 10:22:27 2022 From: ricca.dario at gmail.com (Dario Ricca) Date: Fri, 1 Apr 2022 17:22:27 +0200 Subject: [Histonet] ultrasafe formalin dispense In-Reply-To: References: Message-ID: Hi Nancy, I tested 3 formalin dispenser systems for my lab and the ultrasafe was one of them. This instrument is very easy to use, is one of the safest and the most flexible. It has many sensors that prevent formalin from escaping from the container that you are filling (we stressed this point with different tests), the aspiration is very quiet and is always turned on. It has the possibility to carry 4 collapsible boxes of formalin of 5 L each (or for examples 3 formalin and 1 dH2O or 2 formalin and 1dH2O and 1 PBS (not Carnoy!)) at the same time and allows you to know when you need to change them before it finishes in the way that you can manage easily the change. It's really easy to use, you can set some favourite filling protocols (e.g. 1:3, 1:5, 1:10) that you can choose at any time for any sample. You can manage operators for quality assessment, you can scan your sample QR code. You can print the ID of the sample on the "report label" (the report laber is made out of thermal paper not plastic label) Is the only instrument of the three that you can really push start and walk away. The containers are more expensive due to the silicon valve. Check the size of the instrument before buying, for some laboratories it may be too big. It has air filters but you can connect to an external aspiration system. if you need more info please let me know Dario Ricca Histology Technician Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico - Milano - Italy Il giorno ven 1 apr 2022 alle ore 15:59 Nancy Schmitt via Histonet < histonet at lists.utsouthwestern.edu> ha scritto: > Happy Friday! > Is anyone using the Ultrasafe Formalin Dispensing system? Here is a link > https://www.milestonemedsrl.com/us/product/ultrasafe/ > Appreciate hearing from anyone on what your thoughts are - good or bad. > Thank you much, > Nancy Schmitt HT, MLT(ASCP) > Pathology Support Services > MercyOne Dubuque > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From greg.dobbin at gmail.com Mon Apr 4 07:14:12 2022 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Mon, 4 Apr 2022 09:14:12 -0300 Subject: [Histonet] ultrasafe formalin dispense Message-ID: Honestly, I don't see the point of using this instrument in the histology lab (at least not my lab as we are set up). It would seem odd that we are being "ultra safe" when dispensing the formalin but then it's kind of the "wild west" when we have to discard the previously archived wet tissue! Obviously we are safe when discarding our used formalin but my point is that if there is no closed system for the discard process (none that I am aware of anyway) then why invest in the front end if the back end is still a mess! Now I can see Operating Rooms and Labour and Delivery suites loving this system as many nurses are less comfortable handling formalin. With this system they could safely fill their buckets and send the specimens off to us to let us worry about disposal. Just my 2 cents worth. Maybe someone on here will be able educate me about a safe system for tissue discards. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From michelle.perrins at uct.ac.za Mon Apr 4 07:33:09 2022 From: michelle.perrins at uct.ac.za (Michelle Perrins) Date: Mon, 4 Apr 2022 12:33:09 +0000 Subject: [Histonet] ultrasafe formalin dispense In-Reply-To: References: Message-ID: Hello As a matter of interest, what processes do labs use for the discarding of formlin, xylene and alcohol laboratory waste? Regards Michelle From: Greg Dobbin via Histonet Sent: Monday, April 4, 2022 2:14 PM To: Nancy.Schmitt at mercyhealth.com; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] ultrasafe formalin dispense CAUTION: This email originated outside the UCT network. Do not click any links or open attachments unless you know and trust the source. Honestly, I don't see the point of using this instrument in the histology lab (at least not my lab as we are set up). It would seem odd that we are being "ultra safe" when dispensing the formalin but then it's kind of the "wild west" when we have to discard the previously archived wet tissue! Obviously we are safe when discarding our used formalin but my point is that if there is no closed system for the discard process (none that I am aware of anyway) then why invest in the front end if the back end is still a mess! Now I can see Operating Rooms and Labour and Delivery suites loving this system as many nurses are less comfortable handling formalin. With this system they could safely fill their buckets and send the specimens off to us to let us worry about disposal. Just my 2 cents worth. Maybe someone on here will be able educate me about a safe system for tissue discards. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer - University of Cape Town This email is subject to UCT policies and email disclaimer published on our website at http://www.uct.ac.za/main/email-disclaimer or obtainable from +27 21 650 9111. If this email is not related to the business of UCT, it is sent by the sender in an individual capacity. Please report security incidents or abuse via https://csirt.uct.ac.za/page/report-an-incident.php. From melissa at alliedsearchpartners.com Mon Apr 4 15:45:30 2022 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Mon, 4 Apr 2022 20:45:30 +0000 Subject: [Histonet] Temp/Travel Histotechs Needed in Boca Raton Area- Message-ID: Hello, I have a Travel/Temp Histotech job in Boca Raton area! Position: Histotech Shift: 2pm-10:30pm Monday-Friday Pay: TBD/Discussed on a case by case basis Housing: Housing Offered/Travel Stipend Offered Duration: 4 month contract Temp to Permanent Potential: Yes Please reach out to me if you are available for this contract assignment! Melissa Owens, CHP-ASA Allied Search Partners AN MRINETWORK MEMBER Direct (Call) Line: 407.697.1175 Toll Free: 888.388.7571 ext. 102 From relia1 at earthlink.net Tue Apr 5 12:49:49 2022 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 5 Apr 2022 13:49:49 -0400 Subject: [Histonet] Free Resume Tune up! They're not just for job searches anymore and some exciting opportunities nationwide with bonuses up to 10K + RELIA Exclusives! Message-ID: <007601d84915$8d131ad0$a7395070$@earthlink.net> Hi Histopeeps! Resumes they're not just for job hunting anymore! Did you know it is a great idea to always keep your resume updated? Resumes and CVs are used for many things besides job hunting: Looking at a raise or promotion? Does your resume list all of your accomplishments so that you can present them to your supervisor? Ever thought about giving a presentation or writing an article? A great CV always makes a presentation much more polished. How about just for you? It feels great to see how far you have come in your career and might help with direction for what's next. How about if you are transitioning? Say going back to permanent work from travel or vice versa or want to move into another area of histology. And of course good old fashioned JOB HUNTING! Let me help you get your resume tuned up!! It's free of charge as a service to my Histopeeps!! Here's what you need to do: Reply to this email with I'M IN and we can get started. Job Hunting? Here are some of our Hottest opportunities. All of these are permanent positions. 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From plucas at biopath.org Tue Apr 5 12:55:31 2022 From: plucas at biopath.org (Paula) Date: Tue, 5 Apr 2022 10:55:31 -0700 Subject: [Histonet] Formalin Neutralizer Message-ID: <019801d84916$586a9dc0$093fd940$@biopath.org> Hello, Can you recommend the easiest and most cost-effective product to neutralize the formalin waste from our tissue processors? Thank you, Paula Lucas Bio-Path Medical Group From c.tague at pathologyarts.com Tue Apr 5 14:32:19 2022 From: c.tague at pathologyarts.com (Curt Tague) Date: Tue, 5 Apr 2022 19:32:19 +0000 Subject: [Histonet] Formalin Neutralizer In-Reply-To: <019801d84916$586a9dc0$093fd940$@biopath.org> References: <019801d84916$586a9dc0$093fd940$@biopath.org> Message-ID: I use neutralex from Scigen (www.scigenus.com)... https://www.scigenus.com/product-page/neutralex -----Original Message----- From: Paula via Histonet Sent: Tuesday, April 5, 2022 10:56 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Formalin Neutralizer Hello, Can you recommend the easiest and most cost-effective product to neutralize the formalin waste from our tissue processors? Thank you, Paula Lucas Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=2t7_gY_QgCsQnEhIbW0hGqXohzrIjiJRpXu4w5m7YEQ&m=Ce3kXQbDtBLRyR3Q3uGdJgjEcZd3mw8TP4kB1BZ8gWY&s=GzDUB7drhawt_5m_LnTTqFPwS0gUu9HoIIu2lL6jPqs&e= From cfields at mlkch.org Tue Apr 5 14:36:47 2022 From: cfields at mlkch.org (Carol Fields) Date: Tue, 5 Apr 2022 19:36:47 +0000 Subject: [Histonet] Formalin Neutralizer In-Reply-To: References: <019801d84916$586a9dc0$093fd940$@biopath.org> Message-ID: Neutralex from Scigen - It's the best and actually neutralizes. I use several of their products and pre fills. C. Fields Los Angeles -----Original Message----- From: Curt Tague via Histonet Sent: Tuesday, April 5, 2022 12:32 PM To: Paula ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Formalin Neutralizer I use neutralex from Scigen (https://urldefense.com/v3/__http://www.scigenus.com__;!!Lh6VCqc!UbdGgITm_NZyX_csGPR63Jb2F1TRcMQznHNJ6AUPd2PKx2vmebkq7dpEabGqzA7ZQZOXMSMrClXUTAsox-MAktN3KO9jcw$ )... https://urldefense.com/v3/__https://www.scigenus.com/product-page/neutralex__;!!Lh6VCqc!UbdGgITm_NZyX_csGPR63Jb2F1TRcMQznHNJ6AUPd2PKx2vmebkq7dpEabGqzA7ZQZOXMSMrClXUTAsox-MAktO4H7-Oyw$ -----Original Message----- From: Paula via Histonet Sent: Tuesday, April 5, 2022 10:56 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Formalin Neutralizer Hello, Can you recommend the easiest and most cost-effective product to neutralize the formalin waste from our tissue processors? Thank you, Paula Lucas Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=2t7_gY_QgCsQnEhIbW0hGqXohzrIjiJRpXu4w5m7YEQ&m=Ce3kXQbDtBLRyR3Q3uGdJgjEcZd3mw8TP4kB1BZ8gWY&s=GzDUB7drhawt_5m_LnTTqFPwS0gUu9HoIIu2lL6jPqs&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Lh6VCqc!UbdGgITm_NZyX_csGPR63Jb2F1TRcMQznHNJ6AUPd2PKx2vmebkq7dpEabGqzA7ZQZOXMSMrClXUTAsox-MAktM_bZrTBA$ This email message and any files transmitted are sent with confidentiality in mind and contain privileged or copyright information. You must not present this message to another party without gaining permission from the sender. 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From relia1 at earthlink.net Mon Apr 11 11:19:27 2022 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 11 Apr 2022 12:19:27 -0400 Subject: [Histonet] First Look: Exciting leadership opportunities nationwide including FL, CA, UT, AZ, GA and MO and more!! Message-ID: <034201d84dbf$eb24a810$c16df830$@earthlink.net> Hi Histonetters! How are you? I hope you are having a wonderful day. I have a couple of histology leadership positions and I need your help. I am working with clients in need of Pathology and Histology Managers in the following areas: Utah Arizona Georgia California Florida Missouri Histopeeps, would you be interested in any of these opportunities? 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Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net?? https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #hmuhistopeeps From psjayalakshmy at gmail.com Mon Apr 11 22:58:42 2022 From: psjayalakshmy at gmail.com (jayalakshmy p.s) Date: Tue, 12 Apr 2022 09:28:42 +0530 Subject: [Histonet] Clarification about Immunohistochemistry Message-ID: Hai all Histonetters Please anybody clarify my this doubt if possible. When doing Immunohistochemistry for confirmation of Melanoma with DAB chromogen(brown color)the interpretation is not possible because of obscuring by the dense melanin pigment. We dont have any other color chromogen. I tried ihc after bleaching but the section gets detached even from charged slides. Is there any other effective way to do this? Thanks in advance Regards Dr. P S Jayalakshmy From tony.henwood at health.nsw.gov.au Mon Apr 11 23:28:50 2022 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue, 12 Apr 2022 04:28:50 +0000 Subject: [Histonet] Clarification about Immunohistochemistry In-Reply-To: References: Message-ID: Hi there, I have had good results with Giemsa-like counterstaining (Stefanovi? et al 2013, Ravishankar et al 2016) : Azure blue, when substituted for hematoxylin as a counterstain in immunostain preparation, has been used to help differentiate melanocytes from melanophages. Azure blue preferentially stains cytoplasmic melanin granules blue-green, whereas melanocytes are highlighted by brown DAB chromogen. Melanophages, which contain melanin and lack melanocytic determinants, appear clear with blue-green granules in the cytoplasm (Hillesheim et al 2011). After the slides were bond washed for 4min and rinsed in distilled water, they were stained with a mixture of the following solution: 100 mg of Azure blue (Sigma) in 4 ml of distilled water. Solution 2 was prepared as follows: 0.6ml of 0.1M sodium acetate and 3.4 ml 0.1M acetic acid were added to 27ml distilled water. Both solutions 1 and 2 were combined and 5ml of acetone was added. The slides were incubated for 60 min at room temperature, differentiated in 95% ethanol and dehydrated in several changes of absolute ethanol, followed by clearing in xylene with subsequent mounting (Kamino & Tarn 1991, Hillesheim et al 2011). An alternate method is to counterstain the immunohistochemical reaction with a methylene blue solution (method courtesy of Dr Vince Munro, St Vincents Hospital in Sydney): Staining Solution 2.38gm Sodium acetate 4.7ml Acetic acid 5gm Methylene Blue Make up to 1 litre with distilled water Procedure 1. After immunostaining, wash slides in tap water 2. Stain in Methylene Blue solution for 2 minutes 3. Wash well in water 4. Counterstain in Haematoxylin, wash well & blue as usual. 5. Dehydrate, clear and mount Result: Melanin should stain green-blue. Hillesheim, P. B., Slone, S., Kelley, D., Malone, J., & Bahrami, S. (2011). An immunohistochemical comparison between MiTF and MART?1 with Azure blue counterstaining in the setting of solar lentigo and melanoma in situ. Journal of cutaneous pathology, 38(7), 565-569 Kamino H, Tarn ST (1991) Immunoperoxidase technique modified by counterstain with azure B as a diagnostic aid in evaluating heavily pigmented melanocytic neoplasms. Journal of cutaneous pathology, 18(6):436-439. Ravishankar, S., Nagarajan, P., Curry, J. L., Tetzlaff, M. T., Ivan, D., Torres-Cabala, C. A., ... & Prieto, V. G. (2016). Giemsa is the optimal counterstain for immunohistochemical detection of BRAF V600E mutation status in pigmented melanomas. Journal of cutaneous pathology, 43(8), 722-724. Stefanovi?, D., Stefanovi?, M., & Nikin, Z. (2013). Romanowsky-Giemsa as a counterstain for immunohistochemistry: optimizing a traditional reagent. Biotechnic & Histochemistry, 88(6), 329-335. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: jayalakshmy p.s via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, 12 April 2022 1:59 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Clarification about Immunohistochemistry Hai all Histonetters Please anybody clarify my this doubt if possible. When doing Immunohistochemistry for confirmation of Melanoma with DAB chromogen(brown color)the interpretation is not possible because of obscuring by the dense melanin pigment. We dont have any other color chromogen. I tried ihc after bleaching but the section gets detached even from charged slides. Is there any other effective way to do this? Thanks in advance Regards Dr. P S Jayalakshmy _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From psjayalakshmy at gmail.com Tue Apr 12 07:01:55 2022 From: psjayalakshmy at gmail.com (jayalakshmy p.s) Date: Tue, 12 Apr 2022 17:31:55 +0530 Subject: [Histonet] Clarification about Immunohistochemistry In-Reply-To: References: Message-ID: Thank you so much Susan and Tony. Let me prepare the reagents &try. To Susan- can you please elaborate on the steps of the giemsa method. To Tony Henwood - please tell what is meant by "bond washing" Thanks Dr. Jayalakshmy On Tue, Apr 12, 2022, 9:58 AM Tony Henwood (SCHN) < tony.henwood at health.nsw.gov.au> wrote: > Hi there, > > I have had good results with Giemsa-like counterstaining (Stefanovi? et > al 2013, Ravishankar et al 2016) : > > Azure blue, when substituted for hematoxylin as a counterstain in > immunostain preparation, has been used to help differentiate melanocytes > from melanophages. Azure blue preferentially stains cytoplasmic melanin > granules blue-green, whereas melanocytes are highlighted by brown DAB > chromogen. Melanophages, which contain melanin and lack melanocytic > determinants, appear clear with blue-green granules in the cytoplasm > (Hillesheim et al 2011). > > After the slides were bond washed for 4min and rinsed in distilled water, > they were stained with a mixture of the following solution: 100 mg of Azure > blue (Sigma) in 4 ml of distilled water. Solution 2 was prepared as > follows: 0.6ml of 0.1M sodium acetate and 3.4 ml 0.1M acetic acid were > added to 27ml distilled water. Both solutions 1 and 2 were combined and 5ml > of acetone was added. The slides were incubated for 60 min at room > temperature, differentiated in 95% ethanol and dehydrated in several > changes of absolute ethanol, followed by clearing in xylene with subsequent > mounting (Kamino & Tarn 1991, Hillesheim et al 2011). > > An alternate method is to counterstain the immunohistochemical reaction > with a methylene blue solution (method courtesy of Dr Vince Munro, St > Vincents Hospital in Sydney): > > Staining Solution > 2.38gm Sodium acetate > 4.7ml Acetic acid > 5gm Methylene Blue > Make up to 1 litre with distilled water > > Procedure > 1. After immunostaining, wash slides in tap water > 2. Stain in Methylene Blue solution for 2 minutes > 3. Wash well in water > 4. Counterstain in Haematoxylin, wash well & blue as usual. > 5. Dehydrate, clear and mount > > Result: Melanin should stain green-blue. > > Hillesheim, P. B., Slone, S., Kelley, D., Malone, J., & Bahrami, S. > (2011). An immunohistochemical comparison between MiTF and MART?1 with > Azure blue counterstaining in the setting of solar lentigo and melanoma in > situ. Journal of cutaneous pathology, 38(7), 565-569 > > Kamino H, Tarn ST (1991) Immunoperoxidase technique modified by > counterstain with azure B as a diagnostic aid in evaluating heavily > pigmented melanocytic neoplasms. Journal of cutaneous pathology, > 18(6):436-439. > > Ravishankar, S., Nagarajan, P., Curry, J. L., Tetzlaff, M. T., Ivan, D., > Torres-Cabala, C. A., ... & Prieto, V. G. (2016). Giemsa is the optimal > counterstain for immunohistochemical detection of BRAF V600E mutation > status in pigmented melanomas. Journal of cutaneous pathology, 43(8), > 722-724. > > Stefanovi?, D., Stefanovi?, M., & Nikin, Z. (2013). Romanowsky-Giemsa as a > counterstain for immunohistochemistry: optimizing a traditional reagent. > Biotechnic & Histochemistry, 88(6), 329-335. > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children?s Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: jayalakshmy p.s via Histonet [mailto: > histonet at lists.utsouthwestern.edu] > Sent: Tuesday, 12 April 2022 1:59 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Clarification about Immunohistochemistry > > Hai all Histonetters > Please anybody clarify my this doubt if possible. > When doing Immunohistochemistry for confirmation of Melanoma with DAB > chromogen(brown color)the interpretation is not possible because of > obscuring by the dense melanin pigment. We dont have any other color > chromogen. I tried ihc after bleaching but the section gets detached even > from charged slides. Is there any other effective way to do this? > Thanks in advance > Regards > Dr. P S Jayalakshmy > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain > confidential information. If you are not the intended recipient, please > delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and > are not necessarily the views of NSW Health or any of its entities. > From akbitting at geisinger.edu Tue Apr 12 07:39:17 2022 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Tue, 12 Apr 2022 12:39:17 +0000 Subject: [Histonet] [External] Clarification about Immunohistochemistry In-Reply-To: References: Message-ID: We use an Azure B counterstain. Melanin has a greenish tinge after counterstaining. -----Original Message----- From: jayalakshmy p.s via Histonet Sent: Monday, April 11, 2022 11:59 PM To: histonet at lists.utsouthwestern.edu Subject: [External] [Histonet] Clarification about Immunohistochemistry External: Be careful with links and files. If suspicious, alert the Information Security Office. Questions? Call 570-271-8092 Hai all Histonetters Please anybody clarify my this doubt if possible. When doing Immunohistochemistry for confirmation of Melanoma with DAB chromogen(brown color)the interpretation is not possible because of obscuring by the dense melanin pigment. We dont have any other color chromogen. I tried ihc after bleaching but the section gets detached even from charged slides. Is there any other effective way to do this? Thanks in advance Regards Dr. P S Jayalakshmy _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cakbitting%40geisinger.edu%7Ceafd323c5fa44fdf757908da1c38d5e6%7C37d46c567c664402a16055c2313b910d%7C0%7C0%7C637853327692391791%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=JxGzP940ehU4hgYAjtwoKI5EaLl0CUFjbi7iAvahMYw%3D&reserved=0 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From tony.henwood at health.nsw.gov.au Tue Apr 12 15:35:12 2022 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue, 12 Apr 2022 20:35:12 +0000 Subject: [Histonet] Clarification about Immunohistochemistry In-Reply-To: References: Message-ID: "Bond wash" is the propriety buffer used in the Bond Immunostainer. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________ From: jayalakshmy p.s Sent: 12 April 2022 22:01 To: Tony Henwood (SCHN) Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Clarification about Immunohistochemistry Thank you so much Susan and Tony. Let me prepare the reagents &try. To Susan- can you please elaborate on the steps of the giemsa method. To Tony Henwood - please tell what is meant by "bond washing" Thanks Dr. Jayalakshmy On Tue, Apr 12, 2022, 9:58 AM Tony Henwood (SCHN) > wrote: Hi there, I have had good results with Giemsa-like counterstaining (Stefanovi? et al 2013, Ravishankar et al 2016) : Azure blue, when substituted for hematoxylin as a counterstain in immunostain preparation, has been used to help differentiate melanocytes from melanophages. Azure blue preferentially stains cytoplasmic melanin granules blue-green, whereas melanocytes are highlighted by brown DAB chromogen. Melanophages, which contain melanin and lack melanocytic determinants, appear clear with blue-green granules in the cytoplasm (Hillesheim et al 2011). After the slides were bond washed for 4min and rinsed in distilled water, they were stained with a mixture of the following solution: 100 mg of Azure blue (Sigma) in 4 ml of distilled water. Solution 2 was prepared as follows: 0.6ml of 0.1M sodium acetate and 3.4 ml 0.1M acetic acid were added to 27ml distilled water. Both solutions 1 and 2 were combined and 5ml of acetone was added. The slides were incubated for 60 min at room temperature, differentiated in 95% ethanol and dehydrated in several changes of absolute ethanol, followed by clearing in xylene with subsequent mounting (Kamino & Tarn 1991, Hillesheim et al 2011). An alternate method is to counterstain the immunohistochemical reaction with a methylene blue solution (method courtesy of Dr Vince Munro, St Vincents Hospital in Sydney): Staining Solution 2.38gm Sodium acetate 4.7ml Acetic acid 5gm Methylene Blue Make up to 1 litre with distilled water Procedure 1. After immunostaining, wash slides in tap water 2. Stain in Methylene Blue solution for 2 minutes 3. Wash well in water 4. Counterstain in Haematoxylin, wash well & blue as usual. 5. Dehydrate, clear and mount Result: Melanin should stain green-blue. Hillesheim, P. B., Slone, S., Kelley, D., Malone, J., & Bahrami, S. (2011). An immunohistochemical comparison between MiTF and MART?1 with Azure blue counterstaining in the setting of solar lentigo and melanoma in situ. Journal of cutaneous pathology, 38(7), 565-569 Kamino H, Tarn ST (1991) Immunoperoxidase technique modified by counterstain with azure B as a diagnostic aid in evaluating heavily pigmented melanocytic neoplasms. Journal of cutaneous pathology, 18(6):436-439. Ravishankar, S., Nagarajan, P., Curry, J. L., Tetzlaff, M. T., Ivan, D., Torres-Cabala, C. A., ... & Prieto, V. G. (2016). Giemsa is the optimal counterstain for immunohistochemical detection of BRAF V600E mutation status in pigmented melanomas. Journal of cutaneous pathology, 43(8), 722-724. Stefanovi?, D., Stefanovi?, M., & Nikin, Z. (2013). Romanowsky-Giemsa as a counterstain for immunohistochemistry: optimizing a traditional reagent. Biotechnic & Histochemistry, 88(6), 329-335. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: jayalakshmy p.s via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, 12 April 2022 1:59 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Clarification about Immunohistochemistry Hai all Histonetters Please anybody clarify my this doubt if possible. When doing Immunohistochemistry for confirmation of Melanoma with DAB chromogen(brown color)the interpretation is not possible because of obscuring by the dense melanin pigment. We dont have any other color chromogen. I tried ihc after bleaching but the section gets detached even from charged slides. Is there any other effective way to do this? Thanks in advance Regards Dr. P S Jayalakshmy _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From psjayalakshmy at gmail.com Tue Apr 12 21:08:35 2022 From: psjayalakshmy at gmail.com (jayalakshmy p.s) Date: Wed, 13 Apr 2022 07:38:35 +0530 Subject: [Histonet] Clarification about Immunohistochemistry In-Reply-To: References: Message-ID: Thank you for the clarification and the answers of all others On Wed, Apr 13, 2022, 2:05 AM Tony Henwood (SCHN) < tony.henwood at health.nsw.gov.au> wrote: > "Bond wash" is the propriety buffer used in the Bond Immunostainer. > > *Regards* > *Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)* > *Principal Scientist, the Children?s Hospital at Westmead* > *Adjunct Fellow, School of Medicine, University of Western Sydney* > *Tel: 612 9845 3306* > *Fax: 612 9845 3318* > *Pathology Department* > *the** children's* *hospital* *at westmead* > *Cnr Hawkesbury Road and Hainsworth Street, Westmead* > *Locked Bag 4001, Westmead NSW 2145, AUSTRALIA* > ------------------------------ > *From:* jayalakshmy p.s > *Sent:* 12 April 2022 22:01 > *To:* Tony Henwood (SCHN) > *Cc:* histonet at lists.utsouthwestern.edu > > *Subject:* Re: [Histonet] Clarification about Immunohistochemistry > > Thank you so much Susan and Tony. Let me prepare the reagents &try. > To Susan- can you please elaborate on the steps of the giemsa method. > To Tony Henwood - please tell what is meant by "bond washing" > Thanks > Dr. Jayalakshmy > > On Tue, Apr 12, 2022, 9:58 AM Tony Henwood (SCHN) < > tony.henwood at health.nsw.gov.au> wrote: > > Hi there, > > I have had good results with Giemsa-like counterstaining (Stefanovi? et > al 2013, Ravishankar et al 2016) : > > Azure blue, when substituted for hematoxylin as a counterstain in > immunostain preparation, has been used to help differentiate melanocytes > from melanophages. Azure blue preferentially stains cytoplasmic melanin > granules blue-green, whereas melanocytes are highlighted by brown DAB > chromogen. Melanophages, which contain melanin and lack melanocytic > determinants, appear clear with blue-green granules in the cytoplasm > (Hillesheim et al 2011). > > After the slides were bond washed for 4min and rinsed in distilled water, > they were stained with a mixture of the following solution: 100 mg of Azure > blue (Sigma) in 4 ml of distilled water. Solution 2 was prepared as > follows: 0.6ml of 0.1M sodium acetate and 3.4 ml 0.1M acetic acid were > added to 27ml distilled water. Both solutions 1 and 2 were combined and 5ml > of acetone was added. The slides were incubated for 60 min at room > temperature, differentiated in 95% ethanol and dehydrated in several > changes of absolute ethanol, followed by clearing in xylene with subsequent > mounting (Kamino & Tarn 1991, Hillesheim et al 2011). > > An alternate method is to counterstain the immunohistochemical reaction > with a methylene blue solution (method courtesy of Dr Vince Munro, St > Vincents Hospital in Sydney): > > Staining Solution > 2.38gm Sodium acetate > 4.7ml Acetic acid > 5gm Methylene Blue > Make up to 1 litre with distilled water > > Procedure > 1. After immunostaining, wash slides in tap water > 2. Stain in Methylene Blue solution for 2 minutes > 3. Wash well in water > 4. Counterstain in Haematoxylin, wash well & blue as usual. > 5. Dehydrate, clear and mount > > Result: Melanin should stain green-blue. > > Hillesheim, P. B., Slone, S., Kelley, D., Malone, J., & Bahrami, S. > (2011). An immunohistochemical comparison between MiTF and MART?1 with > Azure blue counterstaining in the setting of solar lentigo and melanoma in > situ. Journal of cutaneous pathology, 38(7), 565-569 > > Kamino H, Tarn ST (1991) Immunoperoxidase technique modified by > counterstain with azure B as a diagnostic aid in evaluating heavily > pigmented melanocytic neoplasms. Journal of cutaneous pathology, > 18(6):436-439. > > Ravishankar, S., Nagarajan, P., Curry, J. L., Tetzlaff, M. T., Ivan, D., > Torres-Cabala, C. A., ... & Prieto, V. G. (2016). Giemsa is the optimal > counterstain for immunohistochemical detection of BRAF V600E mutation > status in pigmented melanomas. Journal of cutaneous pathology, 43(8), > 722-724. > > Stefanovi?, D., Stefanovi?, M., & Nikin, Z. (2013). Romanowsky-Giemsa as a > counterstain for immunohistochemistry: optimizing a traditional reagent. > Biotechnic & Histochemistry, 88(6), 329-335. > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children?s Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > > > -----Original Message----- > From: jayalakshmy p.s via Histonet [mailto: > histonet at lists.utsouthwestern.edu] > Sent: Tuesday, 12 April 2022 1:59 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Clarification about Immunohistochemistry > > Hai all Histonetters > Please anybody clarify my this doubt if possible. > When doing Immunohistochemistry for confirmation of Melanoma with DAB > chromogen(brown color)the interpretation is not possible because of > obscuring by the dense melanin pigment. We dont have any other color > chromogen. I tried ihc after bleaching but the section gets detached even > from charged slides. Is there any other effective way to do this? > Thanks in advance > Regards > Dr. P S Jayalakshmy > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain > confidential information. If you are not the intended recipient, please > delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and > are not necessarily the views of NSW Health or any of its entities. > > > This message is intended for the addressee named and may contain > confidential information. If you are not the intended recipient, please > delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and > are not necessarily the views of NSW Health or any of its entities. > From shannon.gower at pathologyserviceinc.com Wed Apr 13 10:46:46 2022 From: shannon.gower at pathologyserviceinc.com (SEG) Date: Wed, 13 Apr 2022 11:46:46 -0400 Subject: [Histonet] Intelsint tissue processors where are you? Message-ID: Hello out there Histology! Are there any labs currently using the Intelsint processors in the US? Previously in the US under model names such as RTP300, RTPH300, RTP360, TRPH360, and RVG1. Most recently Intelsint models are known by TP300, FTP300, ETP, and EFTP. We recently met a few HTs who love these processors and want to hear more about what experiences have been had. These processors are built like tanks, most not requiring service in 5-7 years despite no PMs! Let me know if you love yours? If you?re having trouble with yours please get in touch with me directly today to discuss how PSi can resolve them. My email is Shannon.Gower at pathologyserviceinc.com Thanks, Shannon Gower Account Executive & Application Specialist Pathology Services Inc. Toll Free: 866-398-9478 Cell: 727-515-8189 Shannon.Gower at pathologyserviceinc.com From POWELL_SA at mercer.edu Wed Apr 13 12:44:42 2022 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Wed, 13 Apr 2022 17:44:42 +0000 Subject: [Histonet] Intelsint tissue processors where are you? In-Reply-To: References: Message-ID: Hi Sannon, I have one. Love it. The cost is more reasonable that you can imagine. Shirley -----Original Message----- From: SEG via Histonet Sent: Wednesday, April 13, 2022 11:47 AM To: histonet at lists.utsouthwestern.edu Cc: Sean Draime Subject: [Histonet] Intelsint tissue processors where are you? Hello out there Histology! Are there any labs currently using the Intelsint processors in the US? Previously in the US under model names such as RTP300, RTPH300, RTP360, TRPH360, and RVG1. Most recently Intelsint models are known by TP300, FTP300, ETP, and EFTP. We recently met a few HTs who love these processors and want to hear more about what experiences have been had. These processors are built like tanks, most not requiring service in 5-7 years despite no PMs! Let me know if you love yours? If you're having trouble with yours please get in touch with me directly today to discuss how PSi can resolve them. My email is Shannon.Gower at pathologyserviceinc.com Thanks, Shannon Gower Account Executive & Application Specialist Pathology Services Inc. Toll Free: 866-398-9478 Cell: 727-515-8189 Shannon.Gower at pathologyserviceinc.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cpowell_sa%40mercer.edu%7C3b8b0c8d9aaf4dfeb2bc08da1d64e089%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C1%7C637854616355941985%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=wzkLje6j%2B4TVkIO74lwj2Oo%2BSG0MMrn4jzqudLYkOMo%3D&reserved=0 From shannon.gower at pathologyserviceinc.com Wed Apr 13 12:56:52 2022 From: shannon.gower at pathologyserviceinc.com (SEG) Date: Wed, 13 Apr 2022 13:56:52 -0400 Subject: [Histonet] Intelsint tissue processors where are you? In-Reply-To: References: Message-ID: Indeed!! Sean and I will be at GSM with one of the ETP series processors. Also plan to have the stainer/coverslipper combo at NSH in Reno. Hope to see everyone there! Shannon Gower Account Executive & Application Specialist Pathology Services Inc. Toll Free: 866-398-9478 Cell: 727-515-8189 Shannon.Gower at pathologyserviceinc.com > On Apr 13, 2022, at 1:53 PM, Shirley A. Powell wrote: > > Hi Shannon, > Are you guys going to bring one to the GSH meeting next week? Looking forward to seeing you and Sean. > Shirley > > -----Original Message----- > From: SEG via Histonet > > Sent: Wednesday, April 13, 2022 11:47 AM > To: histonet at lists.utsouthwestern.edu > Cc: Sean Draime > > Subject: [Histonet] Intelsint tissue processors where are you? > > Hello out there Histology! > > Are there any labs currently using the Intelsint processors in the US? > Previously in the US under model names such as RTP300, RTPH300, RTP360, TRPH360, and RVG1. Most recently Intelsint models are known by TP300, FTP300, ETP, and EFTP. > > We recently met a few HTs who love these processors and want to hear more about what experiences have been had. These processors are built like tanks, most not requiring service in 5-7 years despite no PMs! Let me know if you love yours? > > If you're having trouble with yours please get in touch with me directly today to discuss how PSi can resolve them. My email is Shannon.Gower at pathologyserviceinc.com > > > Thanks, > > Shannon Gower > Account Executive & Application Specialist Pathology Services Inc. > > Toll Free: 866-398-9478 > Cell: 727-515-8189 > Shannon.Gower at pathologyserviceinc.com > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cpowell_sa%40mercer.edu%7C3b8b0c8d9aaf4dfeb2bc08da1d64e089%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C1%7C637854616355941985%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=wzkLje6j%2B4TVkIO74lwj2Oo%2BSG0MMrn4jzqudLYkOMo%3D&reserved=0 From travis at imebinc.com Wed Apr 13 13:34:04 2022 From: travis at imebinc.com (travis at imebinc.com) Date: Wed, 13 Apr 2022 11:34:04 -0700 Subject: [Histonet] Intelsint tissue processors where are you? In-Reply-To: References: Message-ID: <032d01d84f65$0e0e9ec0$2a2bdc40$@imebinc.com> I would look into at the cost of the service contract after the warranty is up. It is an Italian company, they keep having to switch American distributors for some reason. They are not really sold on the 2nd hand market (yet), so there is not much resell value. Also, don?t get melted paraffin or xylene on the touch screen, it will render the unit unless. Travis O?Brien ? International Medical Equipment, Inc. 170 Vallecitos De Oro San Marcos, CA 92069 ? 800.543.8496? Phone 760.761.0859? Fax www.imebinc.com -----Original Message----- From: Shirley A. Powell via Histonet Sent: Wednesday, April 13, 2022 10:45 AM To: SEG ; Histonet Cc: Sean Draime Subject: Re: [Histonet] Intelsint tissue processors where are you? Hi Sannon, I have one. Love it. The cost is more reasonable that you can imagine. Shirley -----Original Message----- From: SEG via Histonet Sent: Wednesday, April 13, 2022 11:47 AM To: histonet at lists.utsouthwestern.edu Cc: Sean Draime Subject: [Histonet] Intelsint tissue processors where are you? Hello out there Histology! Are there any labs currently using the Intelsint processors in the US? Previously in the US under model names such as RTP300, RTPH300, RTP360, TRPH360, and RVG1. Most recently Intelsint models are known by TP300, FTP300, ETP, and EFTP. We recently met a few HTs who love these processors and want to hear more about what experiences have been had. These processors are built like tanks, most not requiring service in 5-7 years despite no PMs! Let me know if you love yours? If you're having trouble with yours please get in touch with me directly today to discuss how PSi can resolve them. My email is Shannon.Gower at pathologyserviceinc.com Thanks, Shannon Gower Account Executive & Application Specialist Pathology Services Inc. Toll Free: 866-398-9478 Cell: 727-515-8189 Shannon.Gower at pathologyserviceinc.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsou thwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cpowell_sa%4 0mercer.edu%7C3b8b0c8d9aaf4dfeb2bc08da1d64e089%7C4fb34d2889b247109bcc30824d1 7fc30%7C0%7C1%7C637854616355941985%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwM DAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=wzkLje6j% 2B4TVkIO74lwj2Oo%2BSG0MMrn4jzqudLYkOMo%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shannon.gower at pathologyserviceinc.com Wed Apr 13 13:51:24 2022 From: shannon.gower at pathologyserviceinc.com (SEG) Date: Wed, 13 Apr 2022 14:51:24 -0400 Subject: [Histonet] Intelsint tissue processors where are you? In-Reply-To: <032d01d84f65$0e0e9ec0$2a2bdc40$@imebinc.com> References: <032d01d84f65$0e0e9ec0$2a2bdc40$@imebinc.com> Message-ID: Yes it is an Italian company, and is used throughout Europe extensively. With the U.S. Import and Training Center all the necessary parts and certified training are in house, service isn?t a problem any longer. Also, the cost of service is marginally less than other OEM service contracts. If anybody wants to sell their instrument, we?ll take it! Shannon Gower Account Executive & Application Specialist Pathology Services Inc. Toll Free: 866-398-9478 Cell: 727-515-8189 Shannon.Gower at pathologyserviceinc.com > On Apr 13, 2022, at 2:34 PM, wrote: > > I would look into at the cost of the service contract after the warranty is > up. It is an Italian company, they keep having to switch American > distributors for some reason. They are not really sold on the 2nd hand > market (yet), so there is not much resell value. Also, don?t get melted > paraffin or xylene on the touch screen, it will render the unit unless. > > > Travis O?Brien > > International Medical Equipment, Inc. > 170 Vallecitos De Oro > San Marcos, CA 92069 > > 800.543.8496 Phone > 760.761.0859 Fax > www.imebinc.com > > -----Original Message----- > From: Shirley A. Powell via Histonet > Sent: Wednesday, April 13, 2022 10:45 AM > To: SEG ; Histonet > > Cc: Sean Draime > Subject: Re: [Histonet] Intelsint tissue processors where are you? > > Hi Sannon, I have one. Love it. The cost is more reasonable that you can > imagine. > Shirley > > -----Original Message----- > From: SEG via Histonet > Sent: Wednesday, April 13, 2022 11:47 AM > To: histonet at lists.utsouthwestern.edu > Cc: Sean Draime > Subject: [Histonet] Intelsint tissue processors where are you? > > Hello out there Histology! > > Are there any labs currently using the Intelsint processors in the US? > Previously in the US under model names such as RTP300, RTPH300, RTP360, > TRPH360, and RVG1. Most recently Intelsint models are known by TP300, > FTP300, ETP, and EFTP. > > We recently met a few HTs who love these processors and want to hear more > about what experiences have been had. These processors are built like > tanks, most not requiring service in 5-7 years despite no PMs! Let me > know if you love yours? > > If you're having trouble with yours please get in touch with me directly > today to discuss how PSi can resolve them. My email is > Shannon.Gower at pathologyserviceinc.com > > > Thanks, > > Shannon Gower > Account Executive & Application Specialist Pathology Services Inc. > > Toll Free: 866-398-9478 > Cell: 727-515-8189 > Shannon.Gower at pathologyserviceinc.com > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsou > thwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cpowell_sa%4 > 0mercer.edu%7C3b8b0c8d9aaf4dfeb2bc08da1d64e089%7C4fb34d2889b247109bcc30824d1 > 7fc30%7C0%7C1%7C637854616355941985%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwM > DAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=wzkLje6j% > 2B4TVkIO74lwj2Oo%2BSG0MMrn4jzqudLYkOMo%3D&reserved=0 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lora at pathinst.com Thu Apr 14 08:25:45 2022 From: lora at pathinst.com (Lora Jauregui) Date: Thu, 14 Apr 2022 13:25:45 +0000 Subject: [Histonet] Microwave Reproducibility Message-ID: Any recommendations on a protocol for testing for reproducibility on our microwave and documenting it for CAP? We are a new lab and just setting up equipment and such. We only use it to heat water and to heat silver solution for our GMS stain. Thank you Sent from Mail for Windows From Donna.Willis at BSWHealth.org Thu Apr 14 08:36:25 2022 From: Donna.Willis at BSWHealth.org (Willis, Donna G) Date: Thu, 14 Apr 2022 13:36:25 +0000 Subject: [Histonet] Microwave Reproducibility In-Reply-To: References: Message-ID: What brand/type of microwave are you using? Donna Willis Anatomic Pathology Manager Baylor Scott&White Health Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile -----Original Message----- From: Lora Jauregui via Histonet Sent: Thursday, April 14, 2022 8:26 AM To: histonet at lists.utsouthwestern.edu Subject: {EXTERNAL} [Histonet] Microwave Reproducibility CAUTION:??This email originated outside of BSWH. The actual sender is (histonet-bounces at lists.utsouthwestern.edu) which may be different from the display address in the From: field. Avoid action unless you know the content is safe. Report suspicious emails using the PhishAlarm button located in your Outlook ribbon. Any recommendations on a protocol for testing for reproducibility on our microwave and documenting it for CAP? We are a new lab and just setting up equipment and such. We only use it to heat water and to heat silver solution for our GMS stain. Thank you Sent from Mail for Windows _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!V1ztpMcrTn81HkgGZSDwsimYHmg-KdaPuEU08OP-2bqskn__3RzH_piJCWAM0fEIbw$ ********************************************************************** The information contained in this e-mail may be privileged, confidential, and/or protected from disclosure. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly prohibited and no waiver of any attorney-client, work product, or other privilege is intended. No binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, is permitted by e-mail without express written confirmation by a duly authorized representative of Baylor Scott & White Health. From Toni.Rathborne at RWJBH.org Thu Apr 14 08:52:01 2022 From: Toni.Rathborne at RWJBH.org (Rathborne, Toni) Date: Thu, 14 Apr 2022 13:52:01 +0000 Subject: [Histonet] Microwave Reproducibility In-Reply-To: References: Message-ID: <3261bee0af5240f481d707d74ffbb9de@RWJBH.org> I would suggest starting with a standard amount of water (50-500 ml) to be tested at predetermined intervals according to your policy. Heat the water and record the temperatures at a minimum of 3 different times. Make sure that you are using a certified thermometer. From one test cycle to the next, you can note the deviation from your initial testing, and show reproducibility this way. ------------------------------------------------------------------------------------ NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. -----Original Message----- From: Willis, Donna G via Histonet Sent: Thursday, April 14, 2022 9:36 AM To: Lora Jauregui ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Microwave Reproducibility *** This is an External Email *** What brand/type of microwave are you using? Donna Willis Anatomic Pathology Manager Baylor Scott&White Health Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile -----Original Message----- From: Lora Jauregui via Histonet Sent: Thursday, April 14, 2022 8:26 AM To: histonet at lists.utsouthwestern.edu Subject: {EXTERNAL} [Histonet] Microwave Reproducibility CAUTION: This email originated outside of BSWH. The actual sender is (histonet-bounces at lists.utsouthwestern.edu) which may be different from the display address in the From: field. Avoid action unless you know the content is safe. Report suspicious emails using the PhishAlarm button located in your Outlook ribbon. Any recommendations on a protocol for testing for reproducibility on our microwave and documenting it for CAP? We are a new lab and just setting up equipment and such. We only use it to heat water and to heat silver solution for our GMS stain. Thank you Sent from Mail for Windows _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!V1ztpMcrTn81HkgGZSDwsimYHmg-KdaPuEU08OP-2bqskn__3RzH_piJCWAM0fEIbw$ ********************************************************************** The information contained in this e-mail may be privileged, confidential, and/or protected from disclosure. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly prohibited and no waiver of any attorney-client, work product, or other privilege is intended. No binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, is permitted by e-mail without express written confirmation by a duly authorized representative of Baylor Scott & White Health. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KU82p_qNqnQ!6ECZ6WtAShIe_MhGr9qrLngXk8xJYj2aHt7oktnGRFCu4CnOB6f-ebxHUu6ip8SmoCjDuOBVSB2d13RiSrplD-AjYb1_sBXEYdQ$ From bonnie_salyer at tedpella.com Thu Apr 14 10:44:56 2022 From: bonnie_salyer at tedpella.com (Bonnie Salyer) Date: Thu, 14 Apr 2022 08:44:56 -0700 Subject: [Histonet] Sr. Life Sciences Product Specialist Message-ID: <000701d85016$97af99c0$c70ecd40$@tedpella.com> Are you a Life Sciences expert? Are you skilled on a Transmission Electron Microscope? Do you have Histology experience? Do you happen to have experience using the PELCO Biowave? If you can answer yes to all of these, you are my 'needle in a haystack' and I would love to talk to you. If you have answer yes to some of these questions, I still want to talk to you! We are seeking a Sr. Life Sciences Product Specialist. See our posting for complete details https://www.linkedin.com/jobs/view/2983097525 Did I mention we live in one of the most beautiful areas ever? Live and work in Redding, California -- big town, with NO big city hassles or traffic, and still affordable enough to buy a nice home - and be surrounded by the beautiful outdoors! #lifescience #transmissionelectronmicroscopy #histology #pelcobiowave Bonnie Salyer, SPHR, SHRM-SCP Human Resources Manager Ted Pella, Inc. bonnie_salyer at tedpella.com (530) 243-2200 x260 office (530) 227-0114 cell (530) 243-8278 fax From jordhood at med.umich.edu Wed Apr 20 12:05:37 2022 From: jordhood at med.umich.edu (Hood, Jordan) Date: Wed, 20 Apr 2022 17:05:37 +0000 Subject: [Histonet] Chromic vs Periodic Acid to oxidize tissues, and use of Thiosemicarbazide, for Grocott's Methenamine Silver (GMS) for fungi? Message-ID: Hi all, A while back I asked you about how to successfully perform Jones' Methenamine Silver (JMS) stain on formalin-fixed paraffin-embedded pig kidneys, and your suggestions to use Thiosemicarbazide (TSC) after oxidation with Periodic Acid solved my issue with the silver solution instability (since the slides didn't have to sit in there for too long) and it yielded great results. I have now been asked to generate a protocol (for my research histology/pathology lab specializing in animal tissues) for Grocott's Methenamine Silver (GMS) for Fungi, and while I plan on more-or-less following the protocol from Rowley Biochemical (https://rowleybiochem.wpengine.com/wp-content/uploads/A-121-Grocotts-Method-GMS-for-Fungi.pdf), I have a couple of questions for you: 1. Will TSC work after oxidation with Chromic Acid (Rowley says to use Chromic Acid)? 2. If TSC won't work after Chromic Acid, can I oxidize using Periodic Acid and then use TSC as I did for JMS? I found one article/abstract (https://pubmed.ncbi.nlm.nih.gov/25861133/) that says that it's possible to use heated Periodic Acid, but most other sources indicate that I should use Chromic Acid. 3. Can I use the same silver solution reagents and mixing procedure as I did in JMS (I already have these in the lab and already know that they work, so ideally I wouldn't have to deviate from my JMS protocol)? * For JMS (mostly taken from Rowley's protocol), I made up and filtered the silver solution immediately before use by mixing: i. 170 ml Methenamine 3% (Rowley; F-396-2) ii. 10 ml Silver Nitrate, 5% (Rowley; F-396-3) and iii. 48 ml Borate Buffer, pH 8.2 (Rowley; F-396-4). * Rowley says that for GMS, I should: i. First, prepare Methenamine-Silver Nitrate Stock: * Silver Nitrate, 5%, Aq. (Rowley; A-121-3A) - 5ml * Methenamine, 3%, Aq. (Rowley; A-121-3B) - 100ml ii. Use chemically clean glassware. Store in refrigerator. iii. Then, to prepare Methenamine-Silver Nitrate Working, mix just prior to use: * Methenamine Silver Nitrate Stock - 25.0ml * Distilled Water - 25.0ml * Sodium Borate, 5%, Aq. (Rowley; A-121-2) - 2.0ml I am certainly open to any general advice that you have for GMS as well. Thank you! Jordan H. University of Michigan Ann Arbor, MI ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues From jdhannasch at gmail.com Wed Apr 20 15:57:53 2022 From: jdhannasch at gmail.com (jdhannasch at gmail.com) Date: Wed, 20 Apr 2022 13:57:53 -0700 Subject: [Histonet] Processor Failure Troubleshooting Message-ID: <1DCDD0C9-3104-413D-9824-1CD4AFC0C1F3@gmail.com> Hello everyone, I?ve been fortunate enough to not deal with too many processor problems like a processor breaking down mid schedule or someone putting the wrong chemicals in wrong spot on the processor, but I want to plan for when these occurrences inevitably happens. Does anyone have any resources they could share about this? From katherine at ka-recruiting.com Wed Apr 20 20:28:14 2022 From: katherine at ka-recruiting.com (Katherine Marano) Date: Wed, 20 Apr 2022 21:28:14 -0400 Subject: [Histonet] New histology opening Message-ID: Hi Histonetters, I hope you are having a nice week. I wanted to reach out to you because I am working with a small, private lab located in Southern Louisiana that is looking to hire a full-time Histotech to work on their night shift performing routine histology duties. They are in a very affordable area and are offering full benefits which include health insurance, 401k, profit sharing, vacation and sick leave. I would love to give you some more information if you are interested in hearing about the opportunity? Let me know!! Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From Lisa.Freeman at fda.hhs.gov Thu Apr 21 07:41:34 2022 From: Lisa.Freeman at fda.hhs.gov (Freeman, Lisa*) Date: Thu, 21 Apr 2022 12:41:34 +0000 Subject: [Histonet] Question regarding posting job openings Message-ID: Is it permitted for a company to post job openings in Histonet? If so, how does that company go about posting it? Lisa Freeman, AAS, HT Histology Supervisor Toxicologic Pathology Associates National Center for Toxicological Research 3900 NCTR RD Jefferson, AR 72079 Phone: 870-543-7234 E-mail: Lisa.Freeman at fda.hhs.gov From John.Shelton at UTSouthwestern.edu Thu Apr 21 07:49:49 2022 From: John.Shelton at UTSouthwestern.edu (John Shelton) Date: Thu, 21 Apr 2022 12:49:49 +0000 Subject: [Histonet] Question regarding posting job openings In-Reply-To: References: Message-ID: Howdy Lisa and Histonet List Members, It is permitted. I think the membership benefits from knowing what opportunities exist in the field. Job postings via recruitment services, as well as those from individual hospitals, commercial, or academic labs are welcome. Logistics on how to accomplish... The poster would need to be a subscriber to Histonet. The message would need to be 60kb or smaller, so I suggest stripping any signature logos from the post. Good luck in your search for the right candidate. JS John M Shelton Core Operations Manager Histonet List Owner & Moderator UT Southwestern Histo Pathology Core 5323 Harry Hines Blvd Dallas, Texas 75390-8573 (214)648-1451 Visit the Core's website at: http://www.utsouthwestern.edu/labs/histo-pathology/ Visit Histonet Archives at: http://www.histosearch.com/histonet.html ________________________________ From: zzz_Tag_histonet-lists Sent: Thursday, April 21, 2022 7:41 AM To: zzz_Tag_histonet-lists Subject: [Histonet] Question regarding posting job openings Is it permitted for a company to post job openings in Histonet? If so, how does that company go about posting it? Lisa Freeman, AAS, HT Histology Supervisor Toxicologic Pathology Associates National Center for Toxicological Research 3900 NCTR RD Jefferson, AR 72079 Phone: 870-543-7234 E-mail: Lisa.Freeman at fda.hhs.gov _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ UT Southwestern Medical Center The future of medicine, today. From Lisa.Freeman at fda.hhs.gov Fri Apr 22 13:51:43 2022 From: Lisa.Freeman at fda.hhs.gov (Freeman, Lisa*) Date: Fri, 22 Apr 2022 18:51:43 +0000 Subject: [Histonet] Histotechnician Position Jefferson, AR Message-ID: Histotechnician Toxicologic Pathology Associates, Inc (TPA) is seeking candidates for a Histotechnician position. TPA, an on-site contractor of the Food and Drug Administration, provides pathology and pathology-related services at the National Center for Toxicological Research (NCTR) in Jefferson, Arkansas. Positions are available for individuals who have an HT or HTL (ASCP) certification or Associates degree and minimum of 3 years' experience in a histology laboratory. An equivalent combination of education and experience may be accepted as a satisfactory substitute for certification or Associates degree. The Histotechnician must possess considerable manual dexterity to be efficient and productive. Routine work can be tedious and repetitive. The Histotechnician will perform basic histology support functions to include processing, embedding, sectioning formalin-fixed paraffin-embedded (FFPE) tissue samples and staining tissue specimens (including special stains) utilizing tissue processors, embedding centers, microtomes, and stainers. Desirable skills include critical thinking and methods development, previous experience with rodent tissue, organoid and membrane histology preparations, frozen tissue sectioning on a cryostat and glycol methacrylate embedding of formalin-fixed tissue and sectioning of plastic blocks with a sliding microtome. Prepares and changes reagents and properly disposes chemical wastes; assists in training personnel as needed; completes detailed documentation; assists with maintenance of equipment; other duties as assigned. Basic computer skills. Learn/know correct anatomic terms. Follow Standard Operating Procedures (SOPs), Good Laboratory Practices (GLPs), and department policies/procedures in the performance of all duties. Maintain safety through appropriate use of personal protective equipment and by observing safe work practices. Good attendance and punctuality are basic requirements of the position. Other related laboratory duties may be requested at the direction of the laboratory supervisor. These duties benefit Pathology and are designed to give the Histotechnician an overall perspective of the flow of work through the Pathology laboratories. Requirements for the position include good hand and eye coordination; capable of reading, understanding, and following laboratory procedures; ability to work in a set environment; must have coordination, dexterity; adept at problem solving; work with high level of accuracy, ability to follow detailed instructions. TPA provides a comprehensive benefits package to its employees and a competitive starting salary commensurate with experience, expertise and skill set. Employment of successful candidates will be contingent upon receipt of a satisfactory drug screening report, obtaining the necessary government security clearance and may be required to undergo physical examination. Drug screening is provided by an off-site third party and is paid for by TPA. Interested candidates should submit their resume and contact information to kimberly.maynard at fda.hhs.gov. Lisa Freeman, AAS, HT Histology Supervisor Toxicologic Pathology Associates National Center for Toxicological Research 3900 NCTR RD Jefferson, AR 72079 Phone: 870-543-7234 E-mail: Lisa.Freeman at fda.hhs.gov From relia1 at earthlink.net Wed Apr 27 10:15:45 2022 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 27 Apr 2022 11:15:45 -0400 Subject: [Histonet] Please help a fellow histotech with their research Message-ID: <009001d85a49$ac6767c0$05363740$@earthlink.net> Hi Histonetters! A fellow Histotech is doing a research project and needs your help with a quick questionnaire. Here are the details: Hi, I am a Doctor of Business Administration (DBA) and Organizational Behavior candidate at the Drexel LeBow School of Business. I am at the stage in the program where I have the opportunity to conduct a research study about how regulatory frameworks might be influencing the work environment context, employee attitude and engagement, and perceived performance in a laboratory setting. The purpose of the study is to gain a better understanding of organizational behaviors and the effects of fairness perception, job satisfaction, and professional identity factors, might have on recruitment and retention withing the laboratory field. I would appreciate your participation in the collection of data. Participation in the study involves filling an online survey that will take approximately 10 minutes. Your participation in this survey is completely voluntary and your responses will be kept strictly anonymous. To take the survey, please click on the following link or copy and paste the link into your Internet browser https://drexel.qualtrics.com/jfe/form/SV_6yC2aO66KYbS48C If you know if any laboratory professionals who may be interested in participating in this research study, could you please forward this link or QR code to them? It would be very beneficial to this study. You are under no obligation to share this information and your participation is strictly confidential. Thank you for your time and cooperation. Your feedback is very important. If you have any questions about the research, please contact Jaime Nieto Sierra at jn827 at drexel.edu . This research has been reviewed according to Drexel University Institutional Review Board (IRB) procedures for research. Warm Regards, Jaime A Nieto Sierra, MHA, HTL(ASCP) CMQIHCCM Doctoral Student at Drexel University LeBow College of Business GSAA Anatomic Pathology Manager Kaiser Foundation Hospitals Northern California Cell Phone # 973-418-6447 Email jn827 at drexel.edu or jaime.nietosierra at gmail.com Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #hmuhistopeeps From relia1 at earthlink.net Wed Apr 27 11:26:19 2022 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 27 Apr 2022 12:26:19 -0400 Subject: [Histonet] Happy Lab Week! It's not too late to celebrate and here are some great ideas and some exciting job opportunities! Message-ID: <024801d85a53$8777cb30$96676190$@earthlink.net> Hello Histopeeps I hope you are doing well. I wanted to send a special bulletin wishing you a Happy National Medical Laboratory Professionals Week. I Hope Your Lab Is Having Some Fun, Informative & Special Events To Celebrate Because You Deserve It! I have heard of some really cool things that labs are doing to mark the occasion including luncheons, costume, trivia and photo contests and displays to educate others about what you do. If you get a chance check out the NSH and ASCP's websites section for Lab week. Here are the links: http://www.ascp.org/labweek Events - National Society for Histotechnology (nsh.org) And the NSH FB page is hosting daily activities here is the list: April 25: #MicroscopeMonday - Show us your fun blocks-smiley faces, animals, etc April 26: #TalkAboutTuesday - Leave us a comment and tell us how you got started in Histotechnology April 27: #WintheDayWednesday - Share your biggest wins in histology April 28: #TBT - Share a picture from the past of you working in the lab April 29: #TGIF - Tell us the top 3 things about working in the lab And don't forget to challenge your colleagues to a Lab Week puzzle. Print the crossword, word scramble, and word search for some friendly lab competition. They will post the answers keys in The Block. I would love to hear what you are doing in your lab to celebrate so please shoot me back an e-mail and let me know. And I can't wait to see you over on Facebook! I also wanted to let you know about some exciting new opportunities I am working on: Here Are The Exciting Opportunities I Am Working On! Leadership/Management: Sarasota, FL Ft. Myers, FL Madison, WI St. Louis, MO Atlanta, GA Modesto, CA Histology Positions: FL - Bradenton - GI lab FL - Ft. Myers - Cancer Diagnostics FL - Port Charlotte - Digital Pathology and Histology FL - Panama City - Dermatopathology CA - Los Angeles - Histology Educator OH - Dayton - Dermatopathology MI - Detroit - Research TN - Knoxville - Histology and Molecular! TX - Houston - Run your own satellite lab UT - Salt Lake City - Digital Pathology and Histology All of my positions are full time permanent positions. Most of my clients offer excellent compensation, benefits and relocation assistance and or sign on bonuses. If you or anyone you know might be interested please contact me. I can be reached immediately on my cell at 407-353-5070 or toll free at 866-607-3542 or at relia1 at earthlink.net If you are interested in looking into making a job change in another area please shoot me an email and let's see what I can do to help! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #hmuhistopeeps From mnwelch at newcomersupply.com Thu Apr 28 16:27:31 2022 From: mnwelch at newcomersupply.com (Michael Welch) Date: Thu, 28 Apr 2022 21:27:31 +0000 Subject: [Histonet] New histology opening Message-ID: Hello Histonet Newcomer Supply is a medical supply company based in Middleton, WI. We manufacture and distribute products for histology and pathology laboratories. A growing company we are hiring a Histology Manager to be a part of our talented and dedicated team. This position requires an individual with experience and passion for histology, histotechnologists, histology products, and patient care. If you have supervised or managed a histology laboratory and have an endless desire for improvement and solutions, then this is a great company for you. If you are interested to learn more please contact me about this opportunity. Best Michael Welch President Newcomer Supply 800-383-7799 Ph | 608-831-0866 Fax mnwelch at newcomersupply.com www.newcomersupply.com