From djemge11 at gmail.com Wed Sep 1 10:49:57 2021 From: djemge11 at gmail.com (Donna Emge) Date: Wed, 1 Sep 2021 10:49:57 -0500 Subject: [Histonet] Anyone Using LaserTrack PH6 or PH8 Tissue Cassette Printer General Data In-Reply-To: References: Message-ID: Is anyone using to the LaserTrack PH6 or PH8 Tissue Cassette Printer from General Data? I am looking for information about how you like it and if there are any issues to consider. Pros and cons. Donna Emge, HT(ASCP) Histology Laboratory Manager Ascension Seton Medical Center Austin, TX djemge11 at gmail.com On Tue, Aug 31, 2021, 12:17 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RELIA HOT Histology Job Alert!! Exciting NEW Opportunities in > Denver, CO, Chicago, IL, Gainesville, FL and San Diego, CA! > These are RELIA EXCLUSIVES!! (Pam Barker) > 2. Cryostat vacuums (Wooten, Jennifer) > 3. need some research help (Horner, Roberta J) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 30 Aug 2021 14:04:15 -0400 > From: "Pam Barker" > To: "Histopeeps Histonet" > Subject: [Histonet] RELIA HOT Histology Job Alert!! Exciting NEW > Opportunities in Denver, CO, Chicago, IL, Gainesville, FL and San > Diego, CA! These are RELIA EXCLUSIVES!! > Message-ID: <01a801d79dc9$72d68e10$5883aa30$@earthlink.net> > Content-Type: text/plain; charset="us-ascii" > > Hi Histopeeps! > I hope this is the beginning of a fantastic week for you. > I wanted to drop a quick post on some pretty amazing new opportunities I am > working on. > All of these are full time permanent Day Shift positions and my clients are > offering excellent compensation and stellar benefits. > Most are offering some sort of relocation/sign on bonus > They are willing to accommodate your travel plans and/or completion of your > travel assignment. > My clients are looking forward to meeting you and showing you the > opportunities that they have to offer. > Here are the locations: > *Chicago, IL* > *Gainesville, FL* > *Denver, CO* > *San Diego, CA* > > I also have some great opportunities in: > *Ft. Myers, FL* > *Panama City, FL* > *Aliso Viejo, CA* > *San Diego, CA* > *Modesto, CA* > *Austin, TX* > *Norfolk, VA* > If you or anyone you know might be interested in hearing more about any of > these opportunities before or after the Labor Day Weekend contact me. > I can be reached toll free at 866-607-3542 or email me at > relia1 at earthlink.net or cell/text me at 407-353-5070. > I hope you have a wonderful Labor Day weekend. > > Thanks-Pam > > Right Time, Right Place, Right Move with RELIA! > Providing excellent service exclusively to the Histology Community! > > Thank You! > Pam M. Barker > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1 at earthlink.net > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > www.facebook.com/PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > check out our latest opportunities at: > http://www.jobvertise.com/members/relia1 > #jobs4myhistopeeps > #ilovemyhistopeeps > #histopeeps > Follow my hashtags and make your day great and your career greater!! > > > > > > ------------------------------ > > Message: 2 > Date: Mon, 30 Aug 2021 20:43:04 +0000 > From: "Wooten, Jennifer" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Cryostat vacuums > Message-ID: > < > B603C480D0D3034BBF606031A22BB9DE0345CE33F3 at trl-mail-00.tricore.org> > Content-Type: text/plain; charset="us-ascii" > > Does anyone use a vacuum to clean out their cryostats, either on or > off-board? > > Jennifer Wooten, BA, BS, HTL (ASCP)CM > Technical Supervisor | Anatomic Pathology | University Hospital > > IMPORTANT: This message originates from TriCore Reference Laboratories or > one of its affiliate organizations or representatives. Please note that the > information contained in this e-mail message (including any attachments) > should be considered confidential and intended for the use of the > individual or entity named appropriately. If the reader of this message is > any other than the individual named above or an agent responsible for > delivery, you are hereby notified that any inappropriate dissemination, > distribution, or copying of this communication is strictly prohibited. If > you have received this communication in error, please notify the sender > immediately. Any errant copy of this message must be deleted. > > > > ------------------------------ > > Message: 3 > Date: Tue, 31 Aug 2021 15:03:07 +0000 > From: "Horner, Roberta J" > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > Subject: [Histonet] need some research help > Message-ID: > < > BN8PR02MB57778B13CA387043700F96198ACC9 at BN8PR02MB5777.namprd02.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > I received the following from a researcher in the forensics department. I > am not sure exactly what he wants and am not sure if this is possible. > > I have been asked to assist a researcher in examining thin sections of > coagulated blood drops in order to distniguish individual drops that were > deposited at different times onto a substrate. The investigator intends to > deposit one drop of fresh blood onto a surface (perhaps paper or paraffin) > and allow the blood to coagulate. After that is coagulated he will deposit > a second drop on top of the first. Once the second drop is also coagulated > he will preserve the sample as recommended for cross sectioning and > evalaution. He intends to cut cross sections of the samples and examine > them in order to determine how coagulation time changes the interface of > the two droplets. The underlying question is "does time/extent of > coagulation of deposited blood affect the deposition pattern of additional > layers of blood on top of the first layer?" If the entire sample was > encased in paraffin would there be a way to cut sections for microscopy, > either with or without staining? > > Roberta J Horner > Penn State > Animal Diagnostic Lab > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 213, Issue 22 > ***************************************** > From Doug.Valarik at sonoraquest.com Wed Sep 1 12:08:47 2021 From: Doug.Valarik at sonoraquest.com (Valarik, Doug-SQL) Date: Wed, 1 Sep 2021 17:08:47 +0000 Subject: [Histonet] Anyone Using LaserTrack PH6 or PH8 Tissue Cassette Printer General Data In-Reply-To: References: Message-ID: We switched to those a couple of years ago. If you are low volume they should work fine for you. We are high volume and own around 20, we have had to have numerous lasers replaced which are very expensive, around $13,000. The first laser went out during warranty which prompted us to get a service contract that covers the laser, they don't typically cover them under the standard contract. I'm not 100% sure but I believe all of the replacement lasers are rebuilt, because I tried to contact the vendor of the laser to get one for less and it seems like they are no longer made. Don't quote me on that though, they might have a special OEM deal with the laser manufacturer. -----Original Message----- From: Donna Emge Sent: Wednesday, September 1, 2021 8:50 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Anyone Using LaserTrack PH6 or PH8 Tissue Cassette Printer General Data Is anyone using to the LaserTrack PH6 or PH8 Tissue Cassette Printer from General Data? I am looking for information about how you like it and if there are any issues to consider. Pros and cons. Donna Emge, HT(ASCP) Histology Laboratory Manager Ascension Seton Medical Center Austin, TX djemge11 at gmail.com On Tue, Aug 31, 2021, 12:17 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li > stinfo/histonet__;!!J-3uQMwJ!dA32wjgVkWztzSovppmICrej2Ogj-nKYunsypfPp5 > P2rXmmNaUxjuYD0Pab74XOUEZBmHew$ or, via email, send a message with > subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RELIA HOT Histology Job Alert!! Exciting NEW Opportunities in > Denver, CO, Chicago, IL, Gainesville, FL and San Diego, CA! > These are RELIA EXCLUSIVES!! (Pam Barker) > 2. Cryostat vacuums (Wooten, Jennifer) > 3. need some research help (Horner, Roberta J) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 30 Aug 2021 14:04:15 -0400 > From: "Pam Barker" > To: "Histopeeps Histonet" > Subject: [Histonet] RELIA HOT Histology Job Alert!! Exciting NEW > Opportunities in Denver, CO, Chicago, IL, Gainesville, FL and San > Diego, CA! These are RELIA EXCLUSIVES!! > Message-ID: <01a801d79dc9$72d68e10$5883aa30$@earthlink.net> > Content-Type: text/plain; charset="us-ascii" > > Hi Histopeeps! > I hope this is the beginning of a fantastic week for you. > I wanted to drop a quick post on some pretty amazing new opportunities > I am working on. > All of these are full time permanent Day Shift positions and my > clients are offering excellent compensation and stellar benefits. > Most are offering some sort of relocation/sign on bonus They are > willing to accommodate your travel plans and/or completion of your > travel assignment. > My clients are looking forward to meeting you and showing you the > opportunities that they have to offer. > Here are the locations: > *Chicago, IL* > *Gainesville, FL* > *Denver, CO* > *San Diego, CA* > > I also have some great opportunities in: > *Ft. Myers, FL* > *Panama City, FL* > *Aliso Viejo, CA* > *San Diego, CA* > *Modesto, CA* > *Austin, TX* > *Norfolk, VA* > If you or anyone you know might be interested in hearing more about > any of these opportunities before or after the Labor Day Weekend contact me. > I can be reached toll free at 866-607-3542 or email me at > relia1 at earthlink.net or cell/text me at 407-353-5070. > I hope you have a wonderful Labor Day weekend. > > Thanks-Pam > > Right Time, Right Place, Right Move with RELIA! > Providing excellent service exclusively to the Histology Community! > > Thank You! > Pam M. Barker > Pam Barker > President/Senior Recruiting Specialist-Histology RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1 at earthlink.net > https://urldefense.com/v3/__https://www.facebook.com/RELIASolutionsfor > histologyprofessionals__;!!J-3uQMwJ!dA32wjgVkWztzSovppmICrej2Ogj-nKYun > sypfPp5P2rXmmNaUxjuYD0Pab74XOUP6NMB6s$ > https://urldefense.com/v3/__http://www.facebook.com/PamBarkerRELIA__;! > !J-3uQMwJ!dA32wjgVkWztzSovppmICrej2Ogj-nKYunsypfPp5P2rXmmNaUxjuYD0Pab7 > 4XOUAvdPeS0$ > https://urldefense.com/v3/__http://www.linkedin.com/in/reliasolutions_ > _;!!J-3uQMwJ!dA32wjgVkWztzSovppmICrej2Ogj-nKYunsypfPp5P2rXmmNaUxjuYD0P > ab74XOU5WW67yc$ > https://urldefense.com/v3/__http://www.twitter.com/pamatrelia__;!!J-3u > QMwJ!dA32wjgVkWztzSovppmICrej2Ogj-nKYunsypfPp5P2rXmmNaUxjuYD0Pab74XOU1 > 9lHGrA$ check out our latest opportunities at: > https://urldefense.com/v3/__http://www.jobvertise.com/members/relia1__ > ;!!J-3uQMwJ!dA32wjgVkWztzSovppmICrej2Ogj-nKYunsypfPp5P2rXmmNaUxjuYD0Pa > b74XOU-QjwMIw$ > #jobs4myhistopeeps > #ilovemyhistopeeps > #histopeeps > Follow my hashtags and make your day great and your career greater!! > > > > > > ------------------------------ > > Message: 2 > Date: Mon, 30 Aug 2021 20:43:04 +0000 > From: "Wooten, Jennifer" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Cryostat vacuums > Message-ID: > < > B603C480D0D3034BBF606031A22BB9DE0345CE33F3 at trl-mail-00.tricore.org> > Content-Type: text/plain; charset="us-ascii" > > Does anyone use a vacuum to clean out their cryostats, either on or > off-board? > > Jennifer Wooten, BA, BS, HTL (ASCP)CM > Technical Supervisor | Anatomic Pathology | University Hospital > > IMPORTANT: This message originates from TriCore Reference Laboratories > or one of its affiliate organizations or representatives. Please note > that the information contained in this e-mail message (including any > attachments) should be considered confidential and intended for the > use of the individual or entity named appropriately. If the reader of > this message is any other than the individual named above or an agent > responsible for delivery, you are hereby notified that any > inappropriate dissemination, distribution, or copying of this > communication is strictly prohibited. If you have received this > communication in error, please notify the sender immediately. Any errant copy of this message must be deleted. > > > > ------------------------------ > > Message: 3 > Date: Tue, 31 Aug 2021 15:03:07 +0000 > From: "Horner, Roberta J" > To: "Histonet (histonet at lists.utsouthwestern.edu)" > > Subject: [Histonet] need some research help > Message-ID: > < > BN8PR02MB57778B13CA387043700F96198ACC9 at BN8PR02MB5777.namprd02.prod.out > look.com > > > > Content-Type: text/plain; charset="us-ascii" > > I received the following from a researcher in the forensics > department. I am not sure exactly what he wants and am not sure if this is possible. > > I have been asked to assist a researcher in examining thin sections of > coagulated blood drops in order to distniguish individual drops that > were deposited at different times onto a substrate. The investigator > intends to deposit one drop of fresh blood onto a surface (perhaps > paper or paraffin) and allow the blood to coagulate. After that is > coagulated he will deposit a second drop on top of the first. Once > the second drop is also coagulated he will preserve the sample as > recommended for cross sectioning and evalaution. He intends to cut > cross sections of the samples and examine them in order to determine > how coagulation time changes the interface of the two droplets. The > underlying question is "does time/extent of coagulation of deposited > blood affect the deposition pattern of additional layers of blood on > top of the first layer?" If the entire sample was encased in paraffin > would there be a way to cut sections for microscopy, either with or without staining? > > Roberta J Horner > Penn State > Animal Diagnostic Lab > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li > stinfo/histonet__;!!J-3uQMwJ!dA32wjgVkWztzSovppmICrej2Ogj-nKYunsypfPp5 > P2rXmmNaUxjuYD0Pab74XOUEZBmHew$ > > ------------------------------ > > End of Histonet Digest, Vol 213, Issue 22 > ***************************************** > From Nancy.Schmitt at mercyhealth.com Wed Sep 1 12:20:01 2021 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Wed, 1 Sep 2021 17:20:01 +0000 Subject: [Histonet] solvent recycling waste Message-ID: What are folks doing with the waste from solvent recycling that contains traces of xylene? Thanks Nancy Mercy, Dubuque Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From john at imebinc.com Wed Sep 1 12:21:17 2021 From: john at imebinc.com (=?utf-8?Q?John_O=E2=80=99Brien?=) Date: Wed, 1 Sep 2021 10:21:17 -0700 Subject: [Histonet] Histonet Digest, Vol 214, Issue 1 In-Reply-To: References: Message-ID: Be careful Cost of cassette are 50 cents each when using general data systems Pathtrade Sent from my iPhone > On Sep 1, 2021, at 10:18 AM, histonet-request at lists.utsouthwestern.edu wrote: > > ?Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Histonet Digest, Vol 213, Issue 22 (Patsy Ruegg) > 2. Anyone Using LaserTrack PH6 or PH8 Tissue Cassette Printer > General Data (Donna Emge) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 31 Aug 2021 17:08:45 +0000 > From: Patsy Ruegg > To: "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Histonet Digest, Vol 213, Issue 22 > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > I would suggest using something like histogel or agar to make layers of blood drops in a tube, then freeze them and make frozen sections. Sounds tricky. > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 39037 N 11th Ave > Phoenix, AZ 85086 > C 720-281-5406 > pruegghm at hotmail.com > Doug Ruegg > C720-281-5407 > douglasr19 at hotmail.com > > ________________________________ > From: histonet-request at lists.utsouthwestern.edu > Sent: Tuesday, August 31, 2021 11:00 AM > To: histonet at lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 213, Issue 22 > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > ------------------------------ > > Message: 2 > Date: Wed, 1 Sep 2021 10:49:57 -0500 > From: Donna Emge > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Anyone Using LaserTrack PH6 or PH8 Tissue Cassette > Printer General Data > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > Is anyone using to the LaserTrack PH6 or PH8 Tissue Cassette Printer from > General Data? I am looking for information about how you like it and if > there are any issues to consider. Pros and cons. > > Donna Emge, HT(ASCP) > Histology Laboratory Manager > Ascension Seton Medical Center > Austin, TX > djemge11 at gmail.com > >> On Tue, Aug 31, 2021, 12:17 PM >> wrote: >> >> Send Histonet mailing list submissions to >> histonet at lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-request at lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-owner at lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. RELIA HOT Histology Job Alert!! Exciting NEW Opportunities in >> Denver, CO, Chicago, IL, Gainesville, FL and San Diego, CA! >> These are RELIA EXCLUSIVES!! (Pam Barker) >> 2. Cryostat vacuums (Wooten, Jennifer) >> 3. need some research help (Horner, Roberta J) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Mon, 30 Aug 2021 14:04:15 -0400 >> From: "Pam Barker" >> To: "Histopeeps Histonet" >> Subject: [Histonet] RELIA HOT Histology Job Alert!! Exciting NEW >> Opportunities in Denver, CO, Chicago, IL, Gainesville, FL and San >> Diego, CA! These are RELIA EXCLUSIVES!! >> Message-ID: <01a801d79dc9$72d68e10$5883aa30$@earthlink.net> >> Content-Type: text/plain; charset="us-ascii" >> >> Hi Histopeeps! >> I hope this is the beginning of a fantastic week for you. >> I wanted to drop a quick post on some pretty amazing new opportunities I am >> working on. >> All of these are full time permanent Day Shift positions and my clients are >> offering excellent compensation and stellar benefits. >> Most are offering some sort of relocation/sign on bonus >> They are willing to accommodate your travel plans and/or completion of your >> travel assignment. >> My clients are looking forward to meeting you and showing you the >> opportunities that they have to offer. >> Here are the locations: >> *Chicago, IL* >> *Gainesville, FL* >> *Denver, CO* >> *San Diego, CA* >> >> I also have some great opportunities in: >> *Ft. Myers, FL* >> *Panama City, FL* >> *Aliso Viejo, CA* >> *San Diego, CA* >> *Modesto, CA* >> *Austin, TX* >> *Norfolk, VA* >> If you or anyone you know might be interested in hearing more about any of >> these opportunities before or after the Labor Day Weekend contact me. >> I can be reached toll free at 866-607-3542 or email me at >> relia1 at earthlink.net or cell/text me at 407-353-5070. >> I hope you have a wonderful Labor Day weekend. >> >> Thanks-Pam >> >> Right Time, Right Place, Right Move with RELIA! >> Providing excellent service exclusively to the Histology Community! >> >> Thank You! >> Pam M. Barker >> Pam Barker >> President/Senior Recruiting Specialist-Histology >> RELIA Solutions >> Specialists in Allied Healthcare Recruiting >> 5703 Red Bug Lake Road #330 >> Winter Springs, FL 32708-4969 >> Phone: (407)657-2027 >> Cell: (407)353-5070 >> FAX: (407)678-2788 >> E-mail: relia1 at earthlink.net >> https://www.facebook.com/RELIASolutionsforhistologyprofessionals >> www.facebook.com/PamBarkerRELIA >> www.linkedin.com/in/reliasolutions >> www.twitter.com/pamatrelia >> check out our latest opportunities at: >> http://www.jobvertise.com/members/relia1 >> #jobs4myhistopeeps >> #ilovemyhistopeeps >> #histopeeps >> Follow my hashtags and make your day great and your career greater!! >> >> >> >> >> >> ------------------------------ >> >> Message: 2 >> Date: Mon, 30 Aug 2021 20:43:04 +0000 >> From: "Wooten, Jennifer" >> To: "histonet at lists.utsouthwestern.edu" >> >> Subject: [Histonet] Cryostat vacuums >> Message-ID: >> < >> B603C480D0D3034BBF606031A22BB9DE0345CE33F3 at trl-mail-00.tricore.org> >> Content-Type: text/plain; charset="us-ascii" >> >> Does anyone use a vacuum to clean out their cryostats, either on or >> off-board? >> >> Jennifer Wooten, BA, BS, HTL (ASCP)CM >> Technical Supervisor | Anatomic Pathology | University Hospital >> >> IMPORTANT: This message originates from TriCore Reference Laboratories or >> one of its affiliate organizations or representatives. Please note that the >> information contained in this e-mail message (including any attachments) >> should be considered confidential and intended for the use of the >> individual or entity named appropriately. If the reader of this message is >> any other than the individual named above or an agent responsible for >> delivery, you are hereby notified that any inappropriate dissemination, >> distribution, or copying of this communication is strictly prohibited. If >> you have received this communication in error, please notify the sender >> immediately. Any errant copy of this message must be deleted. >> >> >> >> ------------------------------ >> >> Message: 3 >> Date: Tue, 31 Aug 2021 15:03:07 +0000 >> From: "Horner, Roberta J" >> To: "Histonet (histonet at lists.utsouthwestern.edu)" >> >> Subject: [Histonet] need some research help >> Message-ID: >> < >> BN8PR02MB57778B13CA387043700F96198ACC9 at BN8PR02MB5777.namprd02.prod.outlook.com >>> >> >> Content-Type: text/plain; charset="us-ascii" >> >> I received the following from a researcher in the forensics department. I >> am not sure exactly what he wants and am not sure if this is possible. >> >> I have been asked to assist a researcher in examining thin sections of >> coagulated blood drops in order to distniguish individual drops that were >> deposited at different times onto a substrate. The investigator intends to >> deposit one drop of fresh blood onto a surface (perhaps paper or paraffin) >> and allow the blood to coagulate. After that is coagulated he will deposit >> a second drop on top of the first. Once the second drop is also coagulated >> he will preserve the sample as recommended for cross sectioning and >> evalaution. He intends to cut cross sections of the samples and examine >> them in order to determine how coagulation time changes the interface of >> the two droplets. The underlying question is "does time/extent of >> coagulation of deposited blood affect the deposition pattern of additional >> layers of blood on top of the first layer?" If the entire sample was >> encased in paraffin would there be a way to cut sections for microscopy, >> either with or without staining? >> >> Roberta J Horner >> Penn State >> Animal Diagnostic Lab >> >> >> ------------------------------ >> >> Subject: Digest Footer >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ------------------------------ >> >> End of Histonet Digest, Vol 213, Issue 22 >> ***************************************** >> > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 214, Issue 1 > **************************************** From Kelly.Pairan at nationwidechildrens.org Thu Sep 2 10:47:26 2021 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Thu, 2 Sep 2021 15:47:26 +0000 Subject: [Histonet] Running animal tissue on instrument validated for human clinical use Message-ID: Good Afternoon, There is an opportunity for our lab to run some animal tissue for a research project. I know I need to take into consideration whether the antibodies are compatible with the species and we should be running control tissue from the same species. Are there CAP rules about running animal tissue on an instrument that used for human clinical use? I feel like I have heard something about this before but couldn't find it in the CAP checklist. Thanks, Kelly Kelly Pairan, BS, HT (ASCP)CM, QIHC (ASCP) Technical Specialist -Anatomic Pathology Department of Pathology and Laboratory Medicine Email: kelly.pairan at nationwidechildrens.org ph: 614-722-5414 fx: 614-722-3033 From simmca at UPMC.EDU Tue Sep 7 06:39:13 2021 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Tue, 7 Sep 2021 11:39:13 +0000 Subject: [Histonet] Mohs Tech needed Message-ID: All, The University of Pittsburgh Medical Center, Physicians Services Division, is looking for a Mohs tech for immediate hire! If interested, please apply at UPMC.com Thank you! Chris Simmons, BS, HTL(ASCP)cm, AS Manager, Dermatopathology and Histology Medical Arts Building 3708 5th Ave Suite 500.11 Pgh, PA 15213 O: 412.864.3880 C: 412.612.0881 F: 412.864.3890 This email may contain confidential information of the sending organization. Any unauthorized or improper disclosure, copying, distribution, or use of the contents of this email and attached document(s) is prohibited. The information contained in this email and attached document(s) is intended only for the personal and confidential use of the recipient(s) named above. If you have received this communication in error, please notify the sender immediately by email and delete the original email and attached document(s). From relia1 at earthlink.net Tue Sep 7 12:54:15 2021 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 7 Sep 2021 13:54:15 -0400 Subject: [Histonet] Educators, Quality Specialists AP leadership and histotechs Exciting opportunities in Florida with up to 10K in bonus/relo Message-ID: <003201d7a411$607e53d0$217afb70$@earthlink.net> Hello Histopeeps, I hope you are having a great week! I am Sooo excited about the opportunities I am working on and here?s why: 1. The Florida license is easier than EVER to get. Here?s the link: www.floridasclinicallabs.gov 2. My clients are excited to meet you and ready to make a decision right away. 3. Most of these positions offer up to 10K in bonuses and relocation assistance!! 4. If you have plans made for time off you don?t have to cancel them!! 5. These are some of the top employers in their areas and I have techs that have been working in some of these places for YEARS! Histopeeps!! I have some unusual opportunities that don?t come along every day: I need: Dermpath Histotechs AP Leadership Histology Trainers/Educators Quality Specialist Histopeeps!! I have some amazing job opportunities in: ? Gainesville, Dermpath Days! ? Panama City Dermpath/Days ? Ft. Myers Dermpath/Days ? Ft. Myers Night Shift Supervisor ? Ft. Myers Quality Specialist/Days ? Ft. Myers Histotech/ all shifts Full time part time per diem ? Ft. Myers Histotech /All Shifts New Grads welcome ? Sarasota Histotechs HT/HTL and new grads ? Sarasota AP Supervisor Days!! And New Opportunities Nationwide coming in on a daily basis!! All of these clients are offering full time permanent positions with excellent compensation packages including VERY competitive pay rates, fantastic benefits, relocation and/or sign on bonuses (Up to 10K) and a great team to work with!! I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542, on my cell at 407-353-5070 or relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From Donna.Willis at BSWHealth.org Wed Sep 8 07:29:36 2021 From: Donna.Willis at BSWHealth.org (Willis, Donna G) Date: Wed, 8 Sep 2021 12:29:36 +0000 Subject: [Histonet] Beaker Tracking vs Vantage Message-ID: Good Morning everyone, I would like to know how many of you are using Beaker Tracking from Epic. We are moving from SoftPathDX to Beaker. Currently we have Vantage totally bi-directional with SoftPathDX for our Tracking/Patient Safety system. Would love to hear from Beaker users about the Beaker Tracking System. If there is someone that had Vantage and went to Beaker can you give detail about the differences between the 2 and your preference. Any helpful hints for a Beaker Build. Thanks and have a Blessed Day, Donna Willis Anatomic Pathology Manager Baylor Scott&White Health Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile ********************************************************************** The information contained in this e-mail may be privileged, confidential, and/or protected from disclosure. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly prohibited and no waiver of any attorney-client, work product, or other privilege is intended. No binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, is permitted by e-mail without express written confirmation by a duly authorized representative of Baylor Scott & White Health. From alida.bailleul at gmail.com Thu Sep 9 02:12:38 2021 From: alida.bailleul at gmail.com (Alida Bailleul) Date: Thu, 9 Sep 2021 15:12:38 +0800 Subject: [Histonet] Best DNA/nuclear fluorochrome for BONE In-Reply-To: References: Message-ID: Dear everyone, I recently stained some paraffin slides of demineralized bone with DAPI. The bone (and the calcified cartilage) was so autofluorescent that the real DAPI signal of the cell nuclei was completely lost. (either it was autofluorescent, or the DAPI was not washed away properly and infiltrated the bone matrix). I read online that it is possible to find a better (more recent) fluorochrome that emits further away for the autofluorescence emission of the tissue. Does anyone have any suggestion of a DNA fluorochrome for bone cells (in paraffin sessions?). Any other insight? The cells were permealized. As a control tissue, I used skin, and the protocol worked perfectly, only the cells were blue, but for the bone of the same animal, everything was blue. So there is a problem with the bone matrix. Thank you for your help in advance All the best -- Dr. Alida M. Bailleul Associate Professor Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences www.ivpp-avianevolution.com & Research Associate of Paleontology, Museum of the Rockies, Montana State University Google Scholar - ResearchGate From Timothy.Morken at ucsf.edu Thu Sep 9 11:26:11 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 9 Sep 2021 16:26:11 +0000 Subject: [Histonet] Retirement in sight! Message-ID: After 40 years in the lab I've decided to retire this year - in a week actually! It has been an interesting 4 decades... I started out in an EM lab after getting a degree in Physiology and then competing a 2 year EM course at Delta College in Stockton, CA - the only dedicated EM program at that time. I started out running a scanning EM lab for an electronics company looking at microchips but after a couple years moved to a hosptial lab in Fresno, CA running their EM lab. I was the only one, so from day one was the "Manager" of the lab! I did about 150 EM cases a year and in those days it was a mix of kidney and tumor cases - there was no IHC yet so some tumor diagnostics depended on EM. I did not have quite enough work to keep me busy so I started hanging out in the histology lab. As with many people in this field the day I started working there was the first I had heard of "histology." At first it was helping set up grossing, coverslipping slides and doing immunofluorescence for the kidney cases (and taking "kodachromes" of the results! Does anyone under 30 know what a Kodachrome is?!). But then our director wanted to bring in IHC and so had a tech from a lab at Cedars Sinai in LA come to teach us how to do it. We did all of 10 stains at first. Of course it was all manual and so had to know what was going on with every step. I didn't use an automated stainer for the first 12 years that I did IHC, and at times was doing 150 slides a day manually. Gradually I ended up doing half time in histology and learned cutting, special stains, muscle histochemistry, immunofluorescence for kidney cases. I decided to work on the HT exam since I was doing all that work anyway. We had a lab of four men - pretty rare, Imagine - and we started a study group to all take the test. We met after work a couple times a week for 6 months pretty much memorizing the Sheehan book. We all took the HT and all but one passed. Later I passed the HTL as well. After 11 years of that I moved on to a job in Saudi Arabia - and my wife and daughter went along. I managed the IHC and muscle lab at King Faisal Specialist Hospital in Riyadh. My wife was lucky enough to get a teaching position at the American School where our daughter was in 9th grade. That made all the difference in our life there because if she had not gotten a job I don't think we would have stayed there 5 years. She would have been stuck doing pretty much nothing. I moved on to managing the histology lab as whole. Living in another country is a great experience, even if it is a totally different culture. It certainly changed our outlook on the world and I would not trade that experience for anything. We also did a lot of travelling during those years - being on "that" side of world makes traveling there much easier! Once we decided to leave Saudi I looked for a job back in the States and was lucky enough to land one at the Centers for Disease Control in Atlanta in their Infectious Disease Pathology division. I worked with 5 infectious disease pathology specialists and a dozen technologists from histotechs to EM techs, to microbiologists to molecular biologists. We worked on routine cases to world-wide outbreak cases. During the 5 years I was there we identified at least one novel human virus every year that caused outbreaks. And that was in addition to numerous cases of outbreaks of known diseases for which we received samples from all over the world. Probably the most notorious case was the anthrax attack after 9/11. Four of us histotechs manned the lab 24 hours a day, 7 days a week for 6 weeks running IHC tests on endless samples while trying to get on top of that case. In the middle of it all the power went out to the facility and we had to work on generator power with temporary lighting set up in the lab and battery packs to keep the equipment running. After 9/11 and then anthrax everyone was thinking it was a bioterror attack by the same group, so things were crazy. When I think of all the efforts we made to enhance our detection and diagnostic capabilities, and all our meetings about how to handle outbreaks, it was hard to see the stumbles the CDC made in this current pandemic. But I can say that we had discussed, studied and predicted pretty much everything that has happened in this Covid 19 era. Indeed, we had the first-hand experience with SARS in the last year I was there, so knew exactly how it could play out. Finally we decided to move back to California and I was able to connect with an old friend to get a position at Lab Vision in Fremont, CA. This company made the Dako Autostainer and also had a large offering of antibodies. We only had 25 people but were doing very well and still had a "Startup" culture. That was a very interesting experience after being on the "customer" side for so long. I got to see a lot of different labs, go to a lot of meetings, make a lot of contacts and travel to many other countries to work with distributors. I would recommend working for vendor to anyone to get a real idea of the whole breadth of our field. Once Lab Vision was taken over by Thermo Fisher it became very corporate I decided to go back to the medical lab and ended up at UCSF managing the histology lab. After a few years of that I moved back to the EM lab after the supervisor there retired and they could not find anyone else. So, I ended up back in the place I began! But it is a great place with great people so has been a very good time the last 12 years. When I think back, I started in the lab when the only automation was the tissue processor. Even our specimen logs were all hand-written. Slide labels were typed on a typewriter - there were no computers. We had a staining run set up by a microtome so that person could move the slides along when the timer went off. And there was no fume hood over it, so the histo lab always smelled of xylene, alcohol and formalin fumes. People from outside the lab would come in and almost keel over. Our lab today has no smell at all - we have so many hoods nothing can escape! And much of the lab is automated - IHC, specials, H&E, embedding machines. I always recommend to any new tech that they learn whatever there is to learn in any lab they happen to be in and to not be limited by a job description. You never know when some seemingly obscure thing you learn will come in handy down the road. Take opportunities as they come up. You never know if you will get that opportunity again. Anyway, it has been a good run. I hope to do some other things in in the histology world in the next few years. You never know what will come up! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From bill at dornandhart.com Thu Sep 9 12:17:08 2021 From: bill at dornandhart.com (Bill Hart) Date: Thu, 9 Sep 2021 12:17:08 -0500 Subject: [Histonet] Plastic embedded histology labs Message-ID: Hello Histoland, For the plastic histology labs dealing with the shortage of MMA: we have MMA available to ship.? Please contact me for more details. Thank you, -- Bill Hart Dorn & Hart Microedge, Inc. Bill at dornandhart.com From victor_tobias at comcast.net Thu Sep 9 12:35:44 2021 From: victor_tobias at comcast.net (Victor Tobias) Date: Thu, 9 Sep 2021 10:35:44 -0700 Subject: [Histonet] Retirement in sight! In-Reply-To: References: Message-ID: Congratulations Tim, It has been a long road. Interesting how we both worked at VMC at different times and I would have to say it was prominent in our careers. It was where I was first exposed to Histology. I have enjoyed your friendship and comradeship. Take care Victor Sent from my iPad > On Sep 9, 2021, at 9:26 AM, Morken, Timothy via Histonet wrote: > > ? > After 40 years in the lab I've decided to retire this year - in a week actually! > > It has been an interesting 4 decades... > > I started out in an EM lab after getting a degree in Physiology and then competing a 2 year EM course at Delta College in Stockton, CA - the only dedicated EM program at that time. I started out running a scanning EM lab for an electronics company looking at microchips but after a couple years moved to a hosptial lab in Fresno, CA running their EM lab. I was the only one, so from day one was the "Manager" of the lab! I did about 150 EM cases a year and in those days it was a mix of kidney and tumor cases - there was no IHC yet so some tumor diagnostics depended on EM. I did not have quite enough work to keep me busy so I started hanging out in the histology lab. As with many people in this field the day I started working there was the first I had heard of "histology." At first it was helping set up grossing, coverslipping slides and doing immunofluorescence for the kidney cases (and taking "kodachromes" of the results! Does anyone under 30 know what a Kodachrome is?!). But then our director wanted to bring in IHC and so had a tech from a lab at Cedars Sinai in LA come to teach us how to do it. We did all of 10 stains at first. Of course it was all manual and so had to know what was going on with every step. I didn't use an automated stainer for the first 12 years that I did IHC, and at times was doing 150 slides a day manually. > > Gradually I ended up doing half time in histology and learned cutting, special stains, muscle histochemistry, immunofluorescence for kidney cases. I decided to work on the HT exam since I was doing all that work anyway. We had a lab of four men - pretty rare, Imagine - and we started a study group to all take the test. We met after work a couple times a week for 6 months pretty much memorizing the Sheehan book. We all took the HT and all but one passed. Later I passed the HTL as well. > > After 11 years of that I moved on to a job in Saudi Arabia - and my wife and daughter went along. I managed the IHC and muscle lab at King Faisal Specialist Hospital in Riyadh. My wife was lucky enough to get a teaching position at the American School where our daughter was in 9th grade. That made all the difference in our life there because if she had not gotten a job I don't think we would have stayed there 5 years. She would have been stuck doing pretty much nothing. I moved on to managing the histology lab as whole. Living in another country is a great experience, even if it is a totally different culture. It certainly changed our outlook on the world and I would not trade that experience for anything. We also did a lot of travelling during those years - being on "that" side of world makes traveling there much easier! > > Once we decided to leave Saudi I looked for a job back in the States and was lucky enough to land one at the Centers for Disease Control in Atlanta in their Infectious Disease Pathology division. I worked with 5 infectious disease pathology specialists and a dozen technologists from histotechs to EM techs, to microbiologists to molecular biologists. We worked on routine cases to world-wide outbreak cases. During the 5 years I was there we identified at least one novel human virus every year that caused outbreaks. And that was in addition to numerous cases of outbreaks of known diseases for which we received samples from all over the world. Probably the most notorious case was the anthrax attack after 9/11. Four of us histotechs manned the lab 24 hours a day, 7 days a week for 6 weeks running IHC tests on endless samples while trying to get on top of that case. In the middle of it all the power went out to the facility and we had to work on generator power with temporary lighting set up in the lab and battery packs to keep the equipment running. After 9/11 and then anthrax everyone was thinking it was a bioterror attack by the same group, so things were crazy. When I think of all the efforts we made to enhance our detection and diagnostic capabilities, and all our meetings about how to handle outbreaks, it was hard to see the stumbles the CDC made in this current pandemic. But I can say that we had discussed, studied and predicted pretty much everything that has happened in this Covid 19 era. Indeed, we had the first-hand experience with SARS in the last year I was there, so knew exactly how it could play out. > > Finally we decided to move back to California and I was able to connect with an old friend to get a position at Lab Vision in Fremont, CA. This company made the Dako Autostainer and also had a large offering of antibodies. We only had 25 people but were doing very well and still had a "Startup" culture. That was a very interesting experience after being on the "customer" side for so long. I got to see a lot of different labs, go to a lot of meetings, make a lot of contacts and travel to many other countries to work with distributors. I would recommend working for vendor to anyone to get a real idea of the whole breadth of our field. > > Once Lab Vision was taken over by Thermo Fisher it became very corporate I decided to go back to the medical lab and ended up at UCSF managing the histology lab. After a few years of that I moved back to the EM lab after the supervisor there retired and they could not find anyone else. So, I ended up back in the place I began! But it is a great place with great people so has been a very good time the last 12 years. > > When I think back, I started in the lab when the only automation was the tissue processor. Even our specimen logs were all hand-written. Slide labels were typed on a typewriter - there were no computers. We had a staining run set up by a microtome so that person could move the slides along when the timer went off. And there was no fume hood over it, so the histo lab always smelled of xylene, alcohol and formalin fumes. People from outside the lab would come in and almost keel over. Our lab today has no smell at all - we have so many hoods nothing can escape! And much of the lab is automated - IHC, specials, H&E, embedding machines. > > I always recommend to any new tech that they learn whatever there is to learn in any lab they happen to be in and to not be limited by a job description. You never know when some seemingly obscure thing you learn will come in handy down the road. Take opportunities as they come up. You never know if you will get that opportunity again. > > Anyway, it has been a good run. I hope to do some other things in in the histology world in the next few years. You never know what will come up! > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Thu Sep 9 12:50:26 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 9 Sep 2021 17:50:26 +0000 Subject: [Histonet] Retirement in sight! In-Reply-To: References: Message-ID: Victor, thanks, and I still appreciate your showing me around your lab and barcoding system when we were setting that up. It helped a lot in how we implemented it. VMC was a good place to learn - a small lab, doing a lot of different things. Besides newer methods in molecular biology I can say that I learned pretty much everything at VMC and since then it has just been refinement. And when I was there Dr Price was interested in developing "super techs" and actually paid out of his own pocket for a couple of us to go to NSH and EM meetings. I have not been anywhere else where that happened! We're actually moving back to Fresno! My wife is from there and has family there. We had looked at other places, and in other states but decided that was the easiest place to go to. What really made me decide to take the plunge was the spike I housing prices this year. We were able to sell and make quite a bit. My wife had serious case of Fear of Missing Out - thought the market would drop and we would lose the gains. So, here we are! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Victor Tobias Sent: Thursday, September 09, 2021 10:36 AM To: Morken, Timothy Cc: Histonet Subject: Re: [Histonet] Retirement in sight! Congratulations Tim, It has been a long road. Interesting how we both worked at VMC at different times and I would have to say it was prominent in our careers. It was where I was first exposed to Histology. I have enjoyed your friendship and comradeship. Take care Victor Sent from my iPad > On Sep 9, 2021, at 9:26 AM, Morken, Timothy via Histonet wrote: > > ? > After 40 years in the lab I've decided to retire this year - in a week actually! > > It has been an interesting 4 decades... > > I started out in an EM lab after getting a degree in Physiology and then competing a 2 year EM course at Delta College in Stockton, CA - the only dedicated EM program at that time. I started out running a scanning EM lab for an electronics company looking at microchips but after a couple years moved to a hosptial lab in Fresno, CA running their EM lab. I was the only one, so from day one was the "Manager" of the lab! I did about 150 EM cases a year and in those days it was a mix of kidney and tumor cases - there was no IHC yet so some tumor diagnostics depended on EM. I did not have quite enough work to keep me busy so I started hanging out in the histology lab. As with many people in this field the day I started working there was the first I had heard of "histology." At first it was helping set up grossing, coverslipping slides and doing immunofluorescence for the kidney cases (and taking "kodachromes" of the results! Does anyone under 30 know what a Kodachrome is?!). But then our director wanted to bring in IHC and so had a tech from a lab at Cedars Sinai in LA come to teach us how to do it. We did all of 10 stains at first. Of course it was all manual and so had to know what was going on with every step. I didn't use an automated stainer for the first 12 years that I did IHC, and at times was doing 150 slides a day manually. > > Gradually I ended up doing half time in histology and learned cutting, special stains, muscle histochemistry, immunofluorescence for kidney cases. I decided to work on the HT exam since I was doing all that work anyway. We had a lab of four men - pretty rare, Imagine - and we started a study group to all take the test. We met after work a couple times a week for 6 months pretty much memorizing the Sheehan book. We all took the HT and all but one passed. Later I passed the HTL as well. > > After 11 years of that I moved on to a job in Saudi Arabia - and my wife and daughter went along. I managed the IHC and muscle lab at King Faisal Specialist Hospital in Riyadh. My wife was lucky enough to get a teaching position at the American School where our daughter was in 9th grade. That made all the difference in our life there because if she had not gotten a job I don't think we would have stayed there 5 years. She would have been stuck doing pretty much nothing. I moved on to managing the histology lab as whole. Living in another country is a great experience, even if it is a totally different culture. It certainly changed our outlook on the world and I would not trade that experience for anything. We also did a lot of travelling during those years - being on "that" side of world makes traveling there much easier! > > Once we decided to leave Saudi I looked for a job back in the States and was lucky enough to land one at the Centers for Disease Control in Atlanta in their Infectious Disease Pathology division. I worked with 5 infectious disease pathology specialists and a dozen technologists from histotechs to EM techs, to microbiologists to molecular biologists. We worked on routine cases to world-wide outbreak cases. During the 5 years I was there we identified at least one novel human virus every year that caused outbreaks. And that was in addition to numerous cases of outbreaks of known diseases for which we received samples from all over the world. Probably the most notorious case was the anthrax attack after 9/11. Four of us histotechs manned the lab 24 hours a day, 7 days a week for 6 weeks running IHC tests on endless samples while trying to get on top of that case. In the middle of it all the power went out to the facility and we had to work on generator power with temporary lighting set up in the lab and battery packs to keep the equipment running. After 9/11 and then anthrax everyone was thinking it was a bioterror attack by the same group, so things were crazy. When I think of all the efforts we made to enhance our detection and diagnostic capabilities, and all our meetings about how to handle outbreaks, it was hard to see the stumbles the CDC made in this current pandemic. But I can say that we had discussed, studied and predicted pretty much everything that has happened in this Covid 19 era. Indeed, we had the first-hand experience with SARS in the last year I was there, so knew exactly how it could play out. > > Finally we decided to move back to California and I was able to connect with an old friend to get a position at Lab Vision in Fremont, CA. This company made the Dako Autostainer and also had a large offering of antibodies. We only had 25 people but were doing very well and still had a "Startup" culture. That was a very interesting experience after being on the "customer" side for so long. I got to see a lot of different labs, go to a lot of meetings, make a lot of contacts and travel to many other countries to work with distributors. I would recommend working for vendor to anyone to get a real idea of the whole breadth of our field. > > Once Lab Vision was taken over by Thermo Fisher it became very corporate I decided to go back to the medical lab and ended up at UCSF managing the histology lab. After a few years of that I moved back to the EM lab after the supervisor there retired and they could not find anyone else. So, I ended up back in the place I began! But it is a great place with great people so has been a very good time the last 12 years. > > When I think back, I started in the lab when the only automation was the tissue processor. Even our specimen logs were all hand-written. Slide labels were typed on a typewriter - there were no computers. We had a staining run set up by a microtome so that person could move the slides along when the timer went off. And there was no fume hood over it, so the histo lab always smelled of xylene, alcohol and formalin fumes. People from outside the lab would come in and almost keel over. Our lab today has no smell at all - we have so many hoods nothing can escape! And much of the lab is automated - IHC, specials, H&E, embedding machines. > > I always recommend to any new tech that they learn whatever there is to learn in any lab they happen to be in and to not be limited by a job description. You never know when some seemingly obscure thing you learn will come in handy down the road. Take opportunities as they come up. You never know if you will get that opportunity again. > > Anyway, it has been a good run. I hope to do some other things in in the histology world in the next few years. You never know what will come up! > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology UC San Francisco Medical Center > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li > stinfo/histonet__;!!LQC6Cpwp!5-R2p1GHuJSQMNi8V50GRnRzuvvOVXs6i8o0KO8SbWM3OGfVEpyzaKFYcTnZR9gqeOvvLg$ From carl.hobbs at kcl.ac.uk Thu Sep 9 13:20:43 2021 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Thu, 9 Sep 2021 18:20:43 +0000 Subject: [Histonet] Retirement in sight! Message-ID: Well, Dr Morken I do not know you but......I have read/absorbed/acted upon occasionally....... your most informative Posts, over the years Your Professional History reads such a wide spectrum of interests/skills/locations! I assume that you will still be contributing to Histonet so that your valuable insights/experiences may still be available to us that have not yet retired. I trust that your Family equally enjoyed your trajectories across the World! I wish you the very best in your new "Post" Sincerely Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6810 From POWELL_SA at mercer.edu Thu Sep 9 14:35:41 2021 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Thu, 9 Sep 2021 19:35:41 +0000 Subject: [Histonet] Tim's Retirement Message-ID: Congratulations Tim, Your world travels have been interesting and for us in the field very rewarding because we all have benefited from your education received in your professional career. I envy your experiences in different parts of the world. All of my 59 years have been in the Macon, GA area. Only 3 institutions, first 2 hospitals and the present one in a medical school. I too hope you will keep hanging with us still working, you have a wealth of knowledge from which we all can benefit. I wish you the best in your retirement. But don't forget about us and keep in touch. Have fun, Shirley Shirley Powell, HTL(ASCP) Technical Director Histology Curricular Support Laboratory Pathology Department Mercer University School of Medicine 1550 College St, Macon, GA 31207 O: 478-301-2374/F:478-301-5489 medicine.mercer.edu -----Original Message----- From: Morken, Timothy via Histonet Sent: Thursday, September 9, 2021 12:26 PM To: Histonet Subject: [Histonet] Retirement in sight! After 40 years in the lab I've decided to retire this year - in a week actually! It has been an interesting 4 decades... I started out in an EM lab after getting a degree in Physiology and then competing a 2 year EM course at Delta College in Stockton, CA - the only dedicated EM program at that time. I started out running a scanning EM lab for an electronics company looking at microchips but after a couple years moved to a hosptial lab in Fresno, CA running their EM lab. I was the only one, so from day one was the "Manager" of the lab! I did about 150 EM cases a year and in those days it was a mix of kidney and tumor cases - there was no IHC yet so some tumor diagnostics depended on EM. I did not have quite enough work to keep me busy so I started hanging out in the histology lab. As with many people in this field the day I started working there was the first I had heard of "histology." At first it was helping set up grossing, coverslipping slides and doing immunofluorescence for the kidney cases (and taking "kodachromes" of the results! Does anyone under 30 know what a Kodachrome is?!). But then our director wanted to bring in IHC and so had a tech from a lab at Cedars Sinai in LA come to teach us how to do it. We did all of 10 stains at first. Of course it was all manual and so had to know what was going on with every step. I didn't use an automated stainer for the first 12 years that I did IHC, and at times was doing 150 slides a day manually. Gradually I ended up doing half time in histology and learned cutting, special stains, muscle histochemistry, immunofluorescence for kidney cases. I decided to work on the HT exam since I was doing all that work anyway. We had a lab of four men - pretty rare, Imagine - and we started a study group to all take the test. We met after work a couple times a week for 6 months pretty much memorizing the Sheehan book. We all took the HT and all but one passed. Later I passed the HTL as well. After 11 years of that I moved on to a job in Saudi Arabia - and my wife and daughter went along. I managed the IHC and muscle lab at King Faisal Specialist Hospital in Riyadh. My wife was lucky enough to get a teaching position at the American School where our daughter was in 9th grade. That made all the difference in our life there because if she had not gotten a job I don't think we would have stayed there 5 years. She would have been stuck doing pretty much nothing. I moved on to managing the histology lab as whole. Living in another country is a great experience, even if it is a totally different culture. It certainly changed our outlook on the world and I would not trade that experience for anything. We also did a lot of travelling during those years - being on "that" side of world makes traveling there much easier! Once we decided to leave Saudi I looked for a job back in the States and was lucky enough to land one at the Centers for Disease Control in Atlanta in their Infectious Disease Pathology division. I worked with 5 infectious disease pathology specialists and a dozen technologists from histotechs to EM techs, to microbiologists to molecular biologists. We worked on routine cases to world-wide outbreak cases. During the 5 years I was there we identified at least one novel human virus every year that caused outbreaks. And that was in addition to numerous cases of outbreaks of known diseases for which we received samples from all over the world. Probably the most notorious case was the anthrax attack after 9/11. Four of us histotechs manned the lab 24 hours a day, 7 days a week for 6 weeks running IHC tests on endless samples while trying to get on top of that case. In the middle of it all the power went out to the facility and we had to work on generator power with temporary lighting set up in the lab and battery packs to keep the equipment running. After 9/11 and then anthrax everyone was thinking it was a bioterror attack by the same group, so things were crazy. When I think of all the efforts we made to enhance our detection and diagnostic capabilities, and all our meetings about how to handle outbreaks, it was hard to see the stumbles the CDC made in this current pandemic. But I can say that we had discussed, studied and predicted pretty much everything that has happened in this Covid 19 era. Indeed, we had the first-hand experience with SARS in the last year I was there, so knew exactly how it could play out. Finally we decided to move back to California and I was able to connect with an old friend to get a position at Lab Vision in Fremont, CA. This company made the Dako Autostainer and also had a large offering of antibodies. We only had 25 people but were doing very well and still had a "Startup" culture. That was a very interesting experience after being on the "customer" side for so long. I got to see a lot of different labs, go to a lot of meetings, make a lot of contacts and travel to many other countries to work with distributors. I would recommend working for vendor to anyone to get a real idea of the whole breadth of our field. Once Lab Vision was taken over by Thermo Fisher it became very corporate I decided to go back to the medical lab and ended up at UCSF managing the histology lab. After a few years of that I moved back to the EM lab after the supervisor there retired and they could not find anyone else. So, I ended up back in the place I began! But it is a great place with great people so has been a very good time the last 12 years. When I think back, I started in the lab when the only automation was the tissue processor. Even our specimen logs were all hand-written. Slide labels were typed on a typewriter - there were no computers. We had a staining run set up by a microtome so that person could move the slides along when the timer went off. And there was no fume hood over it, so the histo lab always smelled of xylene, alcohol and formalin fumes. People from outside the lab would come in and almost keel over. Our lab today has no smell at all - we have so many hoods nothing can escape! And much of the lab is automated - IHC, specials, H&E, embedding machines. I always recommend to any new tech that they learn whatever there is to learn in any lab they happen to be in and to not be limited by a job description. You never know when some seemingly obscure thing you learn will come in handy down the road. Take opportunities as they come up. You never know if you will get that opportunity again. Anyway, it has been a good run. I hope to do some other things in in the histology world in the next few years. You never know what will come up! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cpowell_sa%40mercer.edu%7Cc95befcfb66d4f47665808d973ae9ba6%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C1%7C637668016038704694%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=yjO96DTZFqCsUsuwciRg3YU7VJbVIfkNc%2FCeGRrm1nc%3D&reserved=0 From deewolfe at anatechltdusa.com Thu Sep 9 14:43:24 2021 From: deewolfe at anatechltdusa.com (Dee Wolfe) Date: Thu, 9 Sep 2021 15:43:24 -0400 Subject: [Histonet] Ada's Mail Retirement in sight! In-Reply-To: References: Message-ID: <98C6947C-F565-4D25-AE6F-116C1EBCC438@anatechltdusa.com> Congratulations, Tim?and what a story! Enjoyed "working with you" on the Michigan Society website when Thermo sponsored state society sites! Dee Dee Wolfe MSH Immediate Past President On Sep 9, 2021, at 12:26 PM, Morken, Timothy via Histonet wrote: > > After 40 years in the lab I've decided to retire this year - in a week actually! > > It has been an interesting 4 decades... > > I started out in an EM lab after getting a degree in Physiology and then competing a 2 year EM course at Delta College in Stockton, CA - the only dedicated EM program at that time. I started out running a scanning EM lab for an electronics company looking at microchips but after a couple years moved to a hosptial lab in Fresno, CA running their EM lab. I was the only one, so from day one was the "Manager" of the lab! I did about 150 EM cases a year and in those days it was a mix of kidney and tumor cases - there was no IHC yet so some tumor diagnostics depended on EM. I did not have quite enough work to keep me busy so I started hanging out in the histology lab. As with many people in this field the day I started working there was the first I had heard of "histology." At first it was helping set up grossing, coverslipping slides and doing immunofluorescence for the kidney cases (and taking "kodachromes" of the results! Does anyone under 30 know what a Kodachrome is?!). But then our > director wanted to bring in IHC and so had a tech from a lab at Cedars Sinai in LA come to teach us how to do it. We did all of 10 stains at first. Of course it was all manual and so had to know what was going on with every step. I didn't use an automated stainer for the first 12 years that I did IHC, and at times was doing 150 slides a day manually. > > Gradually I ended up doing half time in histology and learned cutting, special stains, muscle histochemistry, immunofluorescence for kidney cases. I decided to work on the HT exam since I was doing all that work anyway. We had a lab of four men - pretty rare, Imagine - and we started a study group to all take the test. We met after work a couple times a week for 6 months pretty much memorizing the Sheehan book. We all took the HT and all but one passed. Later I passed the HTL as well. > > After 11 years of that I moved on to a job in Saudi Arabia - and my wife and daughter went along. I managed the IHC and muscle lab at King Faisal Specialist Hospital in Riyadh. My wife was lucky enough to get a teaching position at the American School where our daughter was in 9th grade. That made all the difference in our life there because if she had not gotten a job I don't think we would have stayed there 5 years. She would have been stuck doing pretty much nothing. I moved on to managing the histology lab as whole. Living in another country is a great experience, even if it is a totally different culture. It certainly changed our outlook on the world and I would not trade that experience for anything. We also did a lot of travelling during those years - being on "that" side of world makes traveling there much easier! > > Once we decided to leave Saudi I looked for a job back in the States and was lucky enough to land one at the Centers for Disease Control in Atlanta in their Infectious Disease Pathology division. I worked with 5 infectious disease pathology specialists and a dozen technologists from histotechs to EM techs, to microbiologists to molecular biologists. We worked on routine cases to world-wide outbreak cases. During the 5 years I was there we identified at least one novel human virus every year that caused outbreaks. And that was in addition to numerous cases of outbreaks of known diseases for which we received samples from all over the world. Probably the most notorious case was the anthrax attack after 9/11. Four of us histotechs manned the lab 24 hours a day, 7 days a week for 6 weeks running IHC tests on endless samples while trying to get on top of that case. In the middle of it all the power went out to the facility and we had to work on generator power with temporary lighting set > up in the lab and battery packs to keep the equipment running. After 9/11 and then anthrax everyone was thinking it was a bioterror attack by the same group, so things were crazy. When I think of all the efforts we made to enhance our detection and diagnostic capabilities, and all our meetings about how to handle outbreaks, it was hard to see the stumbles the CDC made in this current pandemic. But I can say that we had discussed, studied and predicted pretty much everything that has happened in this Covid 19 era. Indeed, we had the first-hand experience with SARS in the last year I was there, so knew exactly how it could play out. > > Finally we decided to move back to California and I was able to connect with an old friend to get a position at Lab Vision in Fremont, CA. This company made the Dako Autostainer and also had a large offering of antibodies. We only had 25 people but were doing very well and still had a "Startup" culture. That was a very interesting experience after being on the "customer" side for so long. I got to see a lot of different labs, go to a lot of meetings, make a lot of contacts and travel to many other countries to work with distributors. I would recommend working for vendor to anyone to get a real idea of the whole breadth of our field. > > Once Lab Vision was taken over by Thermo Fisher it became very corporate I decided to go back to the medical lab and ended up at UCSF managing the histology lab. After a few years of that I moved back to the EM lab after the supervisor there retired and they could not find anyone else. So, I ended up back in the place I began! But it is a great place with great people so has been a very good time the last 12 years. > > When I think back, I started in the lab when the only automation was the tissue processor. Even our specimen logs were all hand-written. Slide labels were typed on a typewriter - there were no computers. We had a staining run set up by a microtome so that person could move the slides along when the timer went off. And there was no fume hood over it, so the histo lab always smelled of xylene, alcohol and formalin fumes. People from outside the lab would come in and almost keel over. Our lab today has no smell at all - we have so many hoods nothing can escape! And much of the lab is automated - IHC, specials, H&E, embedding machines. > > I always recommend to any new tech that they learn whatever there is to learn in any lab they happen to be in and to not be limited by a job description. You never know when some seemingly obscure thing you learn will come in handy down the road. Take opportunities as they come up. You never know if you will get that opportunity again. > > Anyway, it has been a good run. I hope to do some other things in in the histology world in the next few years. You never know what will come up! > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From 11z at comcast.net Thu Sep 9 15:36:28 2021 From: 11z at comcast.net (11z at comcast.net) Date: Thu, 9 Sep 2021 13:36:28 -0700 Subject: [Histonet] looking for cassettes Message-ID: <000001d7a5ba$62243880$266ca980$@comcast.net> HI. Anybody have a supplier for Klinipath cassettes? I am having a very difficult time finding them these days. Thanks LeRoy Brown HT(ASCP) HTL From brettmc31 at comcast.net Thu Sep 9 16:18:38 2021 From: brettmc31 at comcast.net (brettmc31) Date: Thu, 09 Sep 2021 17:18:38 -0400 Subject: [Histonet] Retirement in sight! In-Reply-To: Message-ID: Hi Tim,Congratulations and welcome to the club... where everyday is a Saturday!Best regards,Brett Connolly, HTL, PhDSent via the Samsung Galaxy S8, an AT&T 5G Evolution capable smartphone -------- Original message --------From: "Morken, Timothy via Histonet" Date: 9/9/21 12:26 PM (GMT-05:00) To: Histonet Subject: [Histonet] Retirement in sight! After 40 years in the lab I've decided to retire this year - in a week actually!It has been an interesting 4 decades...I started out in an EM lab after getting a degree in Physiology and then? competing a 2 year EM course at Delta College in Stockton, CA - the only dedicated EM program at that time. I started out running a scanning EM lab for an electronics company looking at microchips but after a couple years moved to a hosptial lab in Fresno, CA running their EM lab. I was the only one, so from day one was the "Manager" of the lab! I did about 150 EM cases a year and in those days it was a mix of kidney and tumor cases - there was no IHC yet so some tumor diagnostics depended on EM. I did not have quite enough work to keep me busy so I started hanging out in the histology lab. As with many people in this field the day I started working there was the first I had heard of "histology."? At first it was helping set up grossing, coverslipping slides and doing immunofluorescence for the kidney cases (and taking "kodachromes" of the results! Does anyone under 30 know what a Kodachrome is?!). But then our director wanted to bring in IHC and so had a tech from a lab at Cedars Sinai in LA come to teach us how to do it. We did all of 10 stains at first. Of course it was all manual and so had to know what was going on with every step. I didn't use an automated stainer for the first 12 years that I did IHC, and at times was doing 150 slides a day manually.Gradually I ended up doing half time in histology and learned cutting, special stains, muscle histochemistry, immunofluorescence for kidney cases. I decided to work on the HT exam since I was doing all that work anyway. We had a lab of four men - pretty rare, Imagine - and we started a study group to all take the test. We met after work a couple times a week for 6 months pretty? much memorizing the Sheehan book. We all took the HT and all but one passed. Later I passed the HTL as well.After 11 years of that I moved on to a job in Saudi Arabia - and my wife and daughter went along. I managed the IHC and muscle lab at King Faisal Specialist Hospital in Riyadh. My wife was lucky enough to get a teaching position at the American School where our daughter was in 9th grade. That made all the difference in our life there because if she had not gotten a job I don't think we would have stayed there? 5 years. She would have been stuck doing pretty much nothing. I moved on to managing the histology lab as? whole. Living in another country is a great experience, even if it is a totally different culture. It certainly changed our outlook on the world and I would not trade that experience for anything. We also did a lot of travelling during those years - being on "that" side of? world makes traveling there much easier!Once we decided to leave Saudi I looked for a job back in the States and was lucky enough to land one at the Centers for Disease Control in Atlanta in their Infectious Disease Pathology division. I worked with 5 infectious disease pathology specialists and a dozen technologists from histotechs to EM techs, to microbiologists to molecular biologists. We worked on routine cases to world-wide outbreak cases. During the 5 years I was there we identified at least one novel human virus every year that caused outbreaks. And that was in addition to numerous cases of outbreaks of known diseases for which we received samples from all over the world. Probably the most notorious case was the anthrax attack after 9/11. Four of us histotechs manned the lab 24 hours a day, 7 days a week for 6 weeks running IHC tests on endless samples while trying to get on top of that case. In the middle of it all the power went out to the facility and we had to work on generator power with temporary lighting set up in the lab and battery packs to keep the equipment running. After 9/11 and then anthrax everyone was thinking it was a bioterror attack by the same group, so things were crazy. When? I think of all the efforts we made to enhance our detection and diagnostic capabilities, and all our meetings about how to handle outbreaks, it was hard to see the stumbles the CDC made in this current pandemic. But I can say that we had discussed, studied and predicted pretty much everything that has happened in this Covid 19 era. Indeed, we had the first-hand experience with SARS in the last year I was there, so knew exactly how it could play out.Finally we decided to move back to California and I was able to connect with an old friend to get a position at Lab Vision in Fremont, CA. This company made the Dako Autostainer and also had a large offering of antibodies. We only had 25 people but were doing very well and still had a "Startup" culture. That was a very interesting experience after being on the "customer" side for so long. I got to see a lot of different labs, go to a lot of meetings, make a lot of contacts and travel to many other countries to work with distributors. I would recommend working for vendor to anyone to get a real idea of the whole breadth of our field.Once Lab Vision was taken over by Thermo Fisher it became very corporate I decided to go back to the medical lab and ended up at UCSF managing the histology lab. After a few years of that I moved back to the EM lab after the supervisor there retired and they could not find anyone else. So, I ended up back in the place I began! But it is a great place with great people so has been a very good time the last 12 years.When I think back, I started in the lab when the only automation was the tissue processor. Even our specimen logs were all hand-written. Slide labels were typed on a typewriter - there were no computers. We had a staining run set up by a microtome so that person could move the slides along when the timer went off. And there was no fume hood over it, so the histo lab always smelled of? xylene, alcohol and formalin fumes. People from outside the lab would come in and almost keel over. Our lab today has no smell at all - we have so many hoods nothing can escape! And much of the lab is automated - IHC, specials, H&E, embedding machines.I always recommend to any new tech that they learn whatever there is to learn in any lab they happen to be in and to not be limited by a job description. You never know when some seemingly obscure thing you learn will come in handy down the road. Take opportunities as they come up. You never know if you will get that opportunity again.Anyway, it has been a good run. I hope to do some other things in in the histology world in the next few years. You never know what will come up!Tim MorkenSupervisor, Electron Microscopy/Neuromuscular Special StudiesDepartment of PathologyUC San Francisco Medical Center_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From duraine at bcm.edu Thu Sep 9 16:38:50 2021 From: duraine at bcm.edu (Duraine, Lita R) Date: Thu, 9 Sep 2021 21:38:50 +0000 Subject: [Histonet] Retirement in sight! In-Reply-To: References: Message-ID: Congratulations Tim, I too was a graduate of the San Joaquin Delta EM School under Dr Murphy. Your story is similar to most of us. We have been able to go through doors that others only wish they could. Over the years , we often experience many accomplishments, and get to meet many colleagues in the field. I certainly hope that we can continue to see your posts and hear your comments. Enjoy your new journey in life! Best regards, Lita Duraine Certified Electron Microscopist NRI TEM Core Baylor College of Medicine -----Original Message----- From: Morken, Timothy via Histonet Sent: Thursday, September 9, 2021 12:50 PM To: Victor Tobias Cc: Histonet Subject: Re: [Histonet] Retirement in sight! Victor, thanks, and I still appreciate your showing me around your lab and barcoding system when we were setting that up. It helped a lot in how we implemented it. VMC was a good place to learn - a small lab, doing a lot of different things. Besides newer methods in molecular biology I can say that I learned pretty much everything at VMC and since then it has just been refinement. And when I was there Dr Price was interested in developing "super techs" and actually paid out of his own pocket for a couple of us to go to NSH and EM meetings. I have not been anywhere else where that happened! We're actually moving back to Fresno! My wife is from there and has family there. We had looked at other places, and in other states but decided that was the easiest place to go to. What really made me decide to take the plunge was the spike I housing prices this year. We were able to sell and make quite a bit. My wife had serious case of Fear of Missing Out - thought the market would drop and we would lose the gains. So, here we are! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Victor Tobias Sent: Thursday, September 09, 2021 10:36 AM To: Morken, Timothy Cc: Histonet Subject: Re: [Histonet] Retirement in sight! Congratulations Tim, It has been a long road. Interesting how we both worked at VMC at different times and I would have to say it was prominent in our careers. It was where I was first exposed to Histology. I have enjoyed your friendship and comradeship. Take care Victor Sent from my iPad > On Sep 9, 2021, at 9:26 AM, Morken, Timothy via Histonet wrote: > > ? > After 40 years in the lab I've decided to retire this year - in a week actually! > > It has been an interesting 4 decades... > > I started out in an EM lab after getting a degree in Physiology and then competing a 2 year EM course at Delta College in Stockton, CA - the only dedicated EM program at that time. I started out running a scanning EM lab for an electronics company looking at microchips but after a couple years moved to a hosptial lab in Fresno, CA running their EM lab. I was the only one, so from day one was the "Manager" of the lab! I did about 150 EM cases a year and in those days it was a mix of kidney and tumor cases - there was no IHC yet so some tumor diagnostics depended on EM. I did not have quite enough work to keep me busy so I started hanging out in the histology lab. As with many people in this field the day I started working there was the first I had heard of "histology." At first it was helping set up grossing, coverslipping slides and doing immunofluorescence for the kidney cases (and taking "kodachromes" of the results! Does anyone under 30 know what a Kodachrome is?!). But then our director wanted to bring in IHC and so had a tech from a lab at Cedars Sinai in LA come to teach us how to do it. We did all of 10 stains at first. Of course it was all manual and so had to know what was going on with every step. I didn't use an automated stainer for the first 12 years that I did IHC, and at times was doing 150 slides a day manually. > > Gradually I ended up doing half time in histology and learned cutting, special stains, muscle histochemistry, immunofluorescence for kidney cases. I decided to work on the HT exam since I was doing all that work anyway. We had a lab of four men - pretty rare, Imagine - and we started a study group to all take the test. We met after work a couple times a week for 6 months pretty much memorizing the Sheehan book. We all took the HT and all but one passed. Later I passed the HTL as well. > > After 11 years of that I moved on to a job in Saudi Arabia - and my wife and daughter went along. I managed the IHC and muscle lab at King Faisal Specialist Hospital in Riyadh. My wife was lucky enough to get a teaching position at the American School where our daughter was in 9th grade. That made all the difference in our life there because if she had not gotten a job I don't think we would have stayed there 5 years. She would have been stuck doing pretty much nothing. I moved on to managing the histology lab as whole. Living in another country is a great experience, even if it is a totally different culture. It certainly changed our outlook on the world and I would not trade that experience for anything. We also did a lot of travelling during those years - being on "that" side of world makes traveling there much easier! > > Once we decided to leave Saudi I looked for a job back in the States and was lucky enough to land one at the Centers for Disease Control in Atlanta in their Infectious Disease Pathology division. I worked with 5 infectious disease pathology specialists and a dozen technologists from histotechs to EM techs, to microbiologists to molecular biologists. We worked on routine cases to world-wide outbreak cases. During the 5 years I was there we identified at least one novel human virus every year that caused outbreaks. And that was in addition to numerous cases of outbreaks of known diseases for which we received samples from all over the world. Probably the most notorious case was the anthrax attack after 9/11. Four of us histotechs manned the lab 24 hours a day, 7 days a week for 6 weeks running IHC tests on endless samples while trying to get on top of that case. In the middle of it all the power went out to the facility and we had to work on generator power with temporary lighting set up in the lab and battery packs to keep the equipment running. After 9/11 and then anthrax everyone was thinking it was a bioterror attack by the same group, so things were crazy. When I think of all the efforts we made to enhance our detection and diagnostic capabilities, and all our meetings about how to handle outbreaks, it was hard to see the stumbles the CDC made in this current pandemic. But I can say that we had discussed, studied and predicted pretty much everything that has happened in this Covid 19 era. Indeed, we had the first-hand experience with SARS in the last year I was there, so knew exactly how it could play out. > > Finally we decided to move back to California and I was able to connect with an old friend to get a position at Lab Vision in Fremont, CA. This company made the Dako Autostainer and also had a large offering of antibodies. We only had 25 people but were doing very well and still had a "Startup" culture. That was a very interesting experience after being on the "customer" side for so long. I got to see a lot of different labs, go to a lot of meetings, make a lot of contacts and travel to many other countries to work with distributors. I would recommend working for vendor to anyone to get a real idea of the whole breadth of our field. > > Once Lab Vision was taken over by Thermo Fisher it became very corporate I decided to go back to the medical lab and ended up at UCSF managing the histology lab. After a few years of that I moved back to the EM lab after the supervisor there retired and they could not find anyone else. So, I ended up back in the place I began! But it is a great place with great people so has been a very good time the last 12 years. > > When I think back, I started in the lab when the only automation was the tissue processor. Even our specimen logs were all hand-written. Slide labels were typed on a typewriter - there were no computers. We had a staining run set up by a microtome so that person could move the slides along when the timer went off. And there was no fume hood over it, so the histo lab always smelled of xylene, alcohol and formalin fumes. People from outside the lab would come in and almost keel over. Our lab today has no smell at all - we have so many hoods nothing can escape! And much of the lab is automated - IHC, specials, H&E, embedding machines. > > I always recommend to any new tech that they learn whatever there is to learn in any lab they happen to be in and to not be limited by a job description. You never know when some seemingly obscure thing you learn will come in handy down the road. Take opportunities as they come up. You never know if you will get that opportunity again. > > Anyway, it has been a good run. I hope to do some other things in in the histology world in the next few years. You never know what will come up! > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology UC San Francisco Medical Center > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li > stinfo/histonet__;!!LQC6Cpwp!5-R2p1GHuJSQMNi8V50GRnRzuvvOVXs6i8o0KO8Sb > WM3OGfVEpyzaKFYcTnZR9gqeOvvLg$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Z-Quiwr_oQfGYLNUELwQ1A&m=Yz10VGEI3Vq64qr8uave1q0dG6hjI0rBsYLZbk_4-vo&s=lcJlTEZAfZ2sHxZkdh--JgwtZ4ZofvGiPevHBTZkEsU&e= From deewolfe at anatechltdusa.com Thu Sep 9 17:15:25 2021 From: deewolfe at anatechltdusa.com (Dee Wolfe) Date: Thu, 9 Sep 2021 18:15:25 -0400 Subject: [Histonet] Ada's Mail Re: looking for cassettes In-Reply-To: <000001d7a5ba$62243880$266ca980$@comcast.net> References: <000001d7a5ba$62243880$266ca980$@comcast.net> Message-ID: <278C5A9C-DB86-4F1B-968F-6087CED17FE3@anatechltdusa.com> Hi LeRoy, Check out the Cancer Diagnostics website. Not sure what size you are looking for but they carry a similar cassette. https://www.cancerdiagnostics.com/consumables/cassettes Dee Dee Wolfe Anatech Ltd. | VP Technical Service & Manufacturing |1020 Harts Lake Road | Battle Creek, MI 49037 USA | phone +1.800.262.8324 ext 111 | fax +1.269.964.8084 | deewolfe at anatechltdusa.com | www.anatechltdusa.com On Sep 9, 2021, at 4:36 PM, LEROY H BROWN via Histonet wrote: > HI. Anybody have a supplier for Klinipath cassettes? I am having a very > difficult time finding them these days. > > Thanks > > LeRoy Brown HT(ASCP) HTL > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Thu Sep 9 18:19:17 2021 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 9 Sep 2021 23:19:17 +0000 Subject: [Histonet] Retirement in sight! In-Reply-To: References: Message-ID: <038e40b00d634e819cf130acba614de0@SVDCMBX-MEX024.nswhealth.net> I am reminded of the Beatles classic: "Will you still need me, will you still feed me, When I'm sixty-four" 40 years! - a sh.. load of experience, a sh.. load of knowledge. Histotechnology worldwide still needs your wisdom so we hope you can keep an eye on us through Histonet and the Block. Enjoy retirement and hope to see you soon Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 10 September 2021 2:26 AM To: Histonet Subject: [Histonet] Retirement in sight! After 40 years in the lab I've decided to retire this year - in a week actually! It has been an interesting 4 decades... I started out in an EM lab after getting a degree in Physiology and then competing a 2 year EM course at Delta College in Stockton, CA - the only dedicated EM program at that time. I started out running a scanning EM lab for an electronics company looking at microchips but after a couple years moved to a hosptial lab in Fresno, CA running their EM lab. I was the only one, so from day one was the "Manager" of the lab! I did about 150 EM cases a year and in those days it was a mix of kidney and tumor cases - there was no IHC yet so some tumor diagnostics depended on EM. I did not have quite enough work to keep me busy so I started hanging out in the histology lab. As with many people in this field the day I started working there was the first I had heard of "histology." At first it was helping set up grossing, coverslipping slides and doing immunofluorescence for the kidney cases (and taking "kodachromes" of the results! Does anyone under 30 know what a Kodachrome is?!). But then our director wanted to bring in IHC and so had a tech from a lab at Cedars Sinai in LA come to teach us how to do it. We did all of 10 stains at first. Of course it was all manual and so had to know what was going on with every step. I didn't use an automated stainer for the first 12 years that I did IHC, and at times was doing 150 slides a day manually. Gradually I ended up doing half time in histology and learned cutting, special stains, muscle histochemistry, immunofluorescence for kidney cases. I decided to work on the HT exam since I was doing all that work anyway. We had a lab of four men - pretty rare, Imagine - and we started a study group to all take the test. We met after work a couple times a week for 6 months pretty much memorizing the Sheehan book. We all took the HT and all but one passed. Later I passed the HTL as well. After 11 years of that I moved on to a job in Saudi Arabia - and my wife and daughter went along. I managed the IHC and muscle lab at King Faisal Specialist Hospital in Riyadh. My wife was lucky enough to get a teaching position at the American School where our daughter was in 9th grade. That made all the difference in our life there because if she had not gotten a job I don't think we would have stayed there 5 years. She would have been stuck doing pretty much nothing. I moved on to managing the histology lab as whole. Living in another country is a great experience, even if it is a totally different culture. It certainly changed our outlook on the world and I would not trade that experience for anything. We also did a lot of travelling during those years - being on "that" side of world makes traveling there much easier! Once we decided to leave Saudi I looked for a job back in the States and was lucky enough to land one at the Centers for Disease Control in Atlanta in their Infectious Disease Pathology division. I worked with 5 infectious disease pathology specialists and a dozen technologists from histotechs to EM techs, to microbiologists to molecular biologists. We worked on routine cases to world-wide outbreak cases. During the 5 years I was there we identified at least one novel human virus every year that caused outbreaks. And that was in addition to numerous cases of outbreaks of known diseases for which we received samples from all over the world. Probably the most notorious case was the anthrax attack after 9/11. Four of us histotechs manned the lab 24 hours a day, 7 days a week for 6 weeks running IHC tests on endless samples while trying to get on top of that case. In the middle of it all the power went out to the facility and we had to work on generator power with temporary lighting set up in the lab and battery packs to keep the equipment running. After 9/11 and then anthrax everyone was thinking it was a bioterror attack by the same group, so things were crazy. When I think of all the efforts we made to enhance our detection and diagnostic capabilities, and all our meetings about how to handle outbreaks, it was hard to see the stumbles the CDC made in this current pandemic. But I can say that we had discussed, studied and predicted pretty much everything that has happened in this Covid 19 era. Indeed, we had the first-hand experience with SARS in the last year I was there, so knew exactly how it could play out. Finally we decided to move back to California and I was able to connect with an old friend to get a position at Lab Vision in Fremont, CA. This company made the Dako Autostainer and also had a large offering of antibodies. We only had 25 people but were doing very well and still had a "Startup" culture. That was a very interesting experience after being on the "customer" side for so long. I got to see a lot of different labs, go to a lot of meetings, make a lot of contacts and travel to many other countries to work with distributors. I would recommend working for vendor to anyone to get a real idea of the whole breadth of our field. Once Lab Vision was taken over by Thermo Fisher it became very corporate I decided to go back to the medical lab and ended up at UCSF managing the histology lab. After a few years of that I moved back to the EM lab after the supervisor there retired and they could not find anyone else. So, I ended up back in the place I began! But it is a great place with great people so has been a very good time the last 12 years. When I think back, I started in the lab when the only automation was the tissue processor. Even our specimen logs were all hand-written. Slide labels were typed on a typewriter - there were no computers. We had a staining run set up by a microtome so that person could move the slides along when the timer went off. And there was no fume hood over it, so the histo lab always smelled of xylene, alcohol and formalin fumes. People from outside the lab would come in and almost keel over. Our lab today has no smell at all - we have so many hoods nothing can escape! And much of the lab is automated - IHC, specials, H&E, embedding machines. I always recommend to any new tech that they learn whatever there is to learn in any lab they happen to be in and to not be limited by a job description. You never know when some seemingly obscure thing you learn will come in handy down the road. Take opportunities as they come up. You never know if you will get that opportunity again. Anyway, it has been a good run. I hope to do some other things in in the histology world in the next few years. You never know what will come up! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From idimenstein at hotmail.com Thu Sep 9 22:22:56 2021 From: idimenstein at hotmail.com (Izak Dimenstein) Date: Fri, 10 Sep 2021 03:22:56 +0000 Subject: [Histonet] Retirement in sight! In-Reply-To: References: Message-ID: A great man. I was lucky to know you. People like you retire from a job, but not from the histology field. Izak Dimenstein Sent from my iPad > On Sep 9, 2021, at 12:26, Morken, Timothy wrote: > > ? > After 40 years in the lab I've decided to retire this year - in a week actually! > > It has been an interesting 4 decades... > > I started out in an EM lab after getting a degree in Physiology and then competing a 2 year EM course at Delta College in Stockton, CA - the only dedicated EM program at that time. I started out running a scanning EM lab for an electronics company looking at microchips but after a couple years moved to a hosptial lab in Fresno, CA running their EM lab. I was the only one, so from day one was the "Manager" of the lab! I did about 150 EM cases a year and in those days it was a mix of kidney and tumor cases - there was no IHC yet so some tumor diagnostics depended on EM. I did not have quite enough work to keep me busy so I started hanging out in the histology lab. As with many people in this field the day I started working there was the first I had heard of "histology." At first it was helping set up grossing, coverslipping slides and doing immunofluorescence for the kidney cases (and taking "kodachromes" of the results! Does anyone under 30 know what a Kodachrome is?!). But then our director wanted to bring in IHC and so had a tech from a lab at Cedars Sinai in LA come to teach us how to do it. We did all of 10 stains at first. Of course it was all manual and so had to know what was going on with every step. I didn't use an automated stainer for the first 12 years that I did IHC, and at times was doing 150 slides a day manually. > > Gradually I ended up doing half time in histology and learned cutting, special stains, muscle histochemistry, immunofluorescence for kidney cases. I decided to work on the HT exam since I was doing all that work anyway. We had a lab of four men - pretty rare, Imagine - and we started a study group to all take the test. We met after work a couple times a week for 6 months pretty much memorizing the Sheehan book. We all took the HT and all but one passed. Later I passed the HTL as well. > > After 11 years of that I moved on to a job in Saudi Arabia - and my wife and daughter went along. I managed the IHC and muscle lab at King Faisal Specialist Hospital in Riyadh. My wife was lucky enough to get a teaching position at the American School where our daughter was in 9th grade. That made all the difference in our life there because if she had not gotten a job I don't think we would have stayed there 5 years. She would have been stuck doing pretty much nothing. I moved on to managing the histology lab as whole. Living in another country is a great experience, even if it is a totally different culture. It certainly changed our outlook on the world and I would not trade that experience for anything. We also did a lot of travelling during those years - being on "that" side of world makes traveling there much easier! > > Once we decided to leave Saudi I looked for a job back in the States and was lucky enough to land one at the Centers for Disease Control in Atlanta in their Infectious Disease Pathology division. I worked with 5 infectious disease pathology specialists and a dozen technologists from histotechs to EM techs, to microbiologists to molecular biologists. We worked on routine cases to world-wide outbreak cases. During the 5 years I was there we identified at least one novel human virus every year that caused outbreaks. And that was in addition to numerous cases of outbreaks of known diseases for which we received samples from all over the world. Probably the most notorious case was the anthrax attack after 9/11. Four of us histotechs manned the lab 24 hours a day, 7 days a week for 6 weeks running IHC tests on endless samples while trying to get on top of that case. In the middle of it all the power went out to the facility and we had to work on generator power with temporary lighting set up in the lab and battery packs to keep the equipment running. After 9/11 and then anthrax everyone was thinking it was a bioterror attack by the same group, so things were crazy. When I think of all the efforts we made to enhance our detection and diagnostic capabilities, and all our meetings about how to handle outbreaks, it was hard to see the stumbles the CDC made in this current pandemic. But I can say that we had discussed, studied and predicted pretty much everything that has happened in this Covid 19 era. Indeed, we had the first-hand experience with SARS in the last year I was there, so knew exactly how it could play out. > > Finally we decided to move back to California and I was able to connect with an old friend to get a position at Lab Vision in Fremont, CA. This company made the Dako Autostainer and also had a large offering of antibodies. We only had 25 people but were doing very well and still had a "Startup" culture. That was a very interesting experience after being on the "customer" side for so long. I got to see a lot of different labs, go to a lot of meetings, make a lot of contacts and travel to many other countries to work with distributors. I would recommend working for vendor to anyone to get a real idea of the whole breadth of our field. > > Once Lab Vision was taken over by Thermo Fisher it became very corporate I decided to go back to the medical lab and ended up at UCSF managing the histology lab. After a few years of that I moved back to the EM lab after the supervisor there retired and they could not find anyone else. So, I ended up back in the place I began! But it is a great place with great people so has been a very good time the last 12 years. > > When I think back, I started in the lab when the only automation was the tissue processor. Even our specimen logs were all hand-written. Slide labels were typed on a typewriter - there were no computers. We had a staining run set up by a microtome so that person could move the slides along when the timer went off. And there was no fume hood over it, so the histo lab always smelled of xylene, alcohol and formalin fumes. People from outside the lab would come in and almost keel over. Our lab today has no smell at all - we have so many hoods nothing can escape! And much of the lab is automated - IHC, specials, H&E, embedding machines. > > I always recommend to any new tech that they learn whatever there is to learn in any lab they happen to be in and to not be limited by a job description. You never know when some seemingly obscure thing you learn will come in handy down the road. Take opportunities as they come up. You never know if you will get that opportunity again. > > Anyway, it has been a good run. I hope to do some other things in in the histology world in the next few years. You never know what will come up! > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > From tbraud at holyredeemer.com Fri Sep 10 08:23:23 2021 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 10 Sep 2021 13:23:23 +0000 Subject: [Histonet] Happy Retirement, Tim Message-ID: <48E053DDF6CE074DB6A7414BA05403F80223444C64@HRHEX03-HOS.holyredeemer.local> Wishing you all the best in your retirement, Tim. In reading your history in Histology and EM, I can see how your experience has led to so many wise, educational posts on the Histonet. As others have also expressed, I hope you will continue to be a contributing part of the Histology world. Thank you for all the experiences you've shared and congratulations! Sincerely, Terri Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 Honesty AccouNtability AgiLity CoLlaboration CoMpassion From beth.oneil1 at wvumedicine.org Fri Sep 10 09:09:19 2021 From: beth.oneil1 at wvumedicine.org (O'Neil, Beth A.) Date: Fri, 10 Sep 2021 14:09:19 +0000 Subject: [Histonet] Frozen tissue from Immunofluorescence Message-ID: Would anyone be willing to share with me what you do with residual frozen tissue from immunofluorescence testing or frozen tissue remaining from muscle biopsy? CAP requirements only address what you do with residual frozen tissue remaining from intraoperative consultations. We keep the frozen tissue from IF in our -70 C freezer and then periodically dispose of the oldest cases in order to make room for the newer. Does anyone out there actually take the frozen tissue, after a certain period of time, thaw it out and then process into a FFPE block for storage? Beth ONeil beth.oneil1 at wvumedicine.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From relia1 at earthlink.net Mon Sep 13 11:51:01 2021 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 13 Sep 2021 12:51:01 -0400 Subject: [Histonet] Hello Histopeeps! RELIA Histology Career Bulletin Check out positions in FL, NC, CA, CO, IL and more with bonuses of up to 10K Message-ID: <000301d7a8bf$89650d40$9c2f27c0$@earthlink.net> Hello Histopeeps, I hope you are having a great week! I am Sooo excited about the opportunities I am working on and here?s why: 1. The Florida license is easier than EVER to get. Here?s the link: www.floridasclinicallabs.gov 2. My clients are excited to meet you and ready to make a decision right away. 3. Most of these positions offer up to 10K in bonuses and relocation assistance!! 4. If you have plans made for time off you don?t have to cancel them!! 5. These are some of the top employers in their areas and I have techs that have been working in some of these places for YEARS! Histopeeps!! I have some unusual opportunities that don?t come along every day: I need: Dermpath Histotechs AP Leadership Histology Trainers/Educators Quality Specialist Histopeeps!! I have some amazing job opportunities in: ? Gainesville, Dermpath Days! ? Panama City Dermpath/Days ? Ft. Myers Dermpath/Days ? Ft. Myers Night Shift Supervisor ? Ft. Myers Quality Specialist/Days ? Ft. Myers Histotech/ all shifts Full time part time per diem ? Ft. Myers Histotech /All Shifts New Grads welcome ? Sarasota Histotechs HT/HTL and new grads ? Sarasota AP Supervisor Days!! And New Opportunities Nationwide coming in on a daily basis!! All of these clients are offering full time permanent positions with excellent compensation packages including VERY competitive pay rates, fantastic benefits, relocation and/or sign on bonuses (Up to 10K) and a great team to work with!! I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542, on my cell at 407-353-5070 or relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From relia1 at earthlink.net Tue Sep 14 09:45:13 2021 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 14 Sep 2021 10:45:13 -0400 Subject: [Histonet] The NSH Virtual Symposium/Convention starts TODAY! Message-ID: <000001d7a977$20612a00$61237e00$@earthlink.net> Hi Histopeeps! The NSH Virtual Symposium/Convention starts TODAY! Here is the link: www.histoconvention.org Have a great day! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From jamie at watson-home.com Thu Sep 16 14:31:54 2021 From: jamie at watson-home.com (Jamie Watson) Date: Thu, 16 Sep 2021 19:31:54 +0000 Subject: [Histonet] Fwd: Ionis Pharmaceuticals has a fulltime position open in Carlsbad, Ca.: In-Reply-To: References: Message-ID: Hello Everyone, We have a fulltime Research Associate/Senior Research Associate, Histology position https://recruiting.ultipro.com/ISI1000ISIS/JobBoard/14a66400-68fa-4847-8593-af776328cfd2/OpportunityDetail?opportunityId=7ecb097d-bf27-4db4-8d4d-1b6676353338 Ionis is seeking a talented and highly motivated Histotechnician to expand our excellent team. Work in a state-of the art histology lab and join a highly collaborative team. Under general supervision of the Histology Associate Director, prepare microscopic tissue slides for research purposes. Histological procedures such as: tissue preparation, sectioning, histochemical standard and special staining, single/multiplex IHC and ISH staining. Antibody/ISH probe workups, digitalizing glass slides and Image Analysis. Quality control, trouble shooting, adding new ideas/technologies. A great environment to expand your histology knowledge and experience. RESPONSIBILITIES: ? Preparing microscopic slides on human/animal tissue for research/diagnostic purposes, including processing, embedding, microtomy, and mounting to include both paraffin processed tissue and frozen sectioning ? Performing complex histochemical and immunohistochemical stains, data recording, and maintaining instruments ? Quality control and quality assurance procedures, performing routine and special staining ? Slide organization, preparing and maintaining all necessary reagents, including stains, alcohols, and paraffins according to proper specifications ? Handling and disposing of hazardous materials ? Processing specimen accessioning to include all manual and computerized data entry ? Performing other duties as assigned QUALIFICATIONS: ? 3-10 years of Histology laboratory experience ? Proficient with tissue processing, embedding, microtomy, histochemical stains and automated IHC equipment ? Strong interpersonal skills and ability to be successful in a team environment ? Computer data entry (confident/proficient with Word, Excel, SharePoint) ? Understanding and basic use of Image Analysis desired ? Proficiency in algebraic equations ? Willingness to work with hazardous chemicals MINIMUM QUALIFICATIONS: ? Education and experience equivalent to: Associate Degree ? HT(ASCP) Histotechnician Certification desired Jamie Watson Associate Director, Histology jwatson at ionisph.com D: (760)603-2583855 Gazelle Court, Carlsbad, CA 92010 From mcaliger at alliedsearchpartners.com Mon Sep 20 10:07:43 2021 From: mcaliger at alliedsearchpartners.com (Melissa Caliger) Date: Mon, 20 Sep 2021 15:07:43 +0000 Subject: [Histonet] Histotech Temp Needed In Ithaca New York Message-ID: Hello, This is Melissa Caliger with Allied Search Partners I am currently staffing a temporary position in Ithaca, New York for a Histotech Day shift Mon-Fri Housing + Meals + Competitive Payrate offered If you are interested in learning more about the position, please reach out to me, my contact info is below Melissa Caliger Allied Search Partners AN MRINETWORK MEMBER Direct (Call) Line: 386-846-9947 From mim9060 at nyp.org Mon Sep 20 12:06:32 2021 From: mim9060 at nyp.org (Maimone-Schoen, Michele) Date: Mon, 20 Sep 2021 17:06:32 +0000 Subject: [Histonet] Histology Vacancies Message-ID: <4b41c34720084f78a8e1857d380f6d3f@nyp.org> Please post 2 night Histotech Vacancies at New York Presbyterian Hospital - Weill Cornell Division with my contact information below. Thanks Michele Maimone-Schoen, MS Manager, Anatomic Pathology New York-Presbyterian Hospital 525 East 68th Street, Starr 1003 New York, NY 10065 212-746-2633 (Office) 212-746-5007 (Fax) 646-856-1738 (Cell) Be Amazing! ---------------------------------------------------------------------- This electronic message is intended to be for the use only of the named recipient, and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. Thank you. code:d34y From mim9060 at nyp.org Mon Sep 20 15:11:16 2021 From: mim9060 at nyp.org (Maimone-Schoen, Michele) Date: Mon, 20 Sep 2021 20:11:16 +0000 Subject: [Histonet] Histology Supervisor 1 p - 9 p NYP Weill Cornell, Message-ID: <8ea6d4adabeb4b6cb8d6cd267aaaa4d9@nyp.org> Can you please post this position on the site: Histology Supervisor Shift 1 p - 9 p New York Presbyterian Hospital/Weill Cornell Division 525 E. 68th Street NY 10065 Respond/inquire to information below - thank you. Michele Maimone-Schoen, MS Manager, Anatomic Pathology New York-Presbyterian Hospital 525 East 68th Street, Starr 1003 New York, NY 10065 212-746-2633 (Office) 212-746-5007 (Fax) 646-856-1738 (Cell) Be Amazing! ---------------------------------------------------------------------- This electronic message is intended to be for the use only of the named recipient, and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. Thank you. code:d34y From amurvosh at advancederm.net Wed Sep 22 12:36:46 2021 From: amurvosh at advancederm.net (Anne Murvosh) Date: Wed, 22 Sep 2021 17:36:46 +0000 Subject: [Histonet] Mohs Tech Wage? Message-ID: We are trying to hire a part-time Mohs technician in Washington state that is a train on the job. I feel like the wage is too low, what are beginning Mohs techs making if they are not a histology tech? Thanks Anne HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return or destruction. From jaylundgren at gmail.com Wed Sep 22 13:23:49 2021 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 22 Sep 2021 13:23:49 -0500 Subject: [Histonet] Mohs Tech Wage? In-Reply-To: References: Message-ID: Depends on the qualifications you're looking for. If it's just a B.S., or less, then you're competing with Wal-Mart and Burger King and everyone else. The market sets the rate at about $15-$20 bucks an hour, as far as I know, outside of top 10 cities and West coast, right now. Just because you're planning to train them to be a Mohs tech doesn't make them anything special, right now. If the circumstances are special, e.g. you're in San Francisco, you need to pay them more. Just so people can afford to live/commute there. From rmccormick10 at yahoo.com Wed Sep 22 13:26:33 2021 From: rmccormick10 at yahoo.com (Rhonda McCormick) Date: Wed, 22 Sep 2021 18:26:33 +0000 (UTC) Subject: [Histonet] AP References: <1345081427.757653.1632335193786.ref@mail.yahoo.com> Message-ID: <1345081427.757653.1632335193786@mail.yahoo.com> Would anyone please share any information regarding staining FFPE tissue with Alkaline Phosphatase for osteosarcomas. Is it solely performed by IHC or are there any special stains that could be used?TIA Rhonda McCormick BS, HT (ASCP)cm Histology Diagnostic?Lab Supervisor College of Veterinary Medicine Texas A&M University Lab:?979.845.5149rmccormick10 at tamu.edu From LIHANSON at lhs.org Wed Sep 22 13:28:47 2021 From: LIHANSON at lhs.org (Hanson, Leslie I :LLS Lab) Date: Wed, 22 Sep 2021 18:28:47 +0000 Subject: [Histonet] Multiple H&E stainer maintenance Message-ID: Hi all, We recently acquired a second H&E stainer and are working up a maintenance log. Looking for input from others who have already tackled this issue! * Do you have a log for EACH instrument or one combined log? * Do you have a space for listing reagent lots? * What is your rotation schedule for changing reagents? * Other tips and tricks are always appreciated! Thanks! Leslie Hanson, HT(ASCP) Tech Specialist IHC / Pathology Phone: (503)944-7923 lihanson at lhs.org From Charles.Bacon at baystatehealth.org Thu Sep 23 12:17:42 2021 From: Charles.Bacon at baystatehealth.org (Bacon, Charles) Date: Thu, 23 Sep 2021 17:17:42 +0000 Subject: [Histonet] Multiple H&E stainer maintenance In-Reply-To: References: Message-ID: <2211e9fd3b204d3790182cdbf0026398@ZXSWEXCHMXPR06.bhs.org> Hi Leslie, I have attached our current log. You can augment it based off the solutions you are using and how many shifts you have. We have two stainers and each has their own log. Our stainers are connected to running water thus the drain lines that need cleaning. If you have any questions reach out anytime. Good luck! Best, Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786? Fax: 413-322-4790 Charles.Bacon at baystatehealth.org -----Original Message----- From: Hanson, Leslie I :LLS Lab [mailto:LIHANSON at lhs.org] Sent: Wednesday, September 22, 2021 2:29 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Multiple H&E stainer maintenance Hi all, We recently acquired a second H&E stainer and are working up a maintenance log. Looking for input from others who have already tackled this issue! * Do you have a log for EACH instrument or one combined log? * Do you have a space for listing reagent lots? * What is your rotation schedule for changing reagents? * Other tips and tricks are always appreciated! Thanks! Leslie Hanson, HT(ASCP) Tech Specialist IHC / Pathology Phone: (503)944-7923 lihanson at lhs.org ---------------------------------------------------------------------- Please view our annual report at http://www.bhannualreport.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at 413-794-0000 and destroy all copies of this communication and any attachments. For further information regarding Baystate Health's privacy policy, please visit our Internet site at https://www.baystatehealth.org. From jordhood at med.umich.edu Thu Sep 23 14:49:52 2021 From: jordhood at med.umich.edu (Hood, Jordan) Date: Thu, 23 Sep 2021 19:49:52 +0000 Subject: [Histonet] Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions Message-ID: <48bd31f00b074079baca10d963e1bc2f@med.umich.edu> Hello, I'm new to histology (and new to histonet), and I work in a small histology lab specializing in animal tissues that receives requests/submissions from researchers. I tried (and failed) to perform a Jones' Methenamine Silver stain on a client's submission of pig kidneys (formalin-fixed, paraffin-embedded, cut at 2.5 microns), and I need some help troubleshooting this stain since my co-workers are stumped, too. I used the following procedure from Rowley Biochemical: ~~~~~ "Fixation: 10% Buffered Neutral Formalin (F-113) or Bouin's Solution (F-40) or Zenker's (F-155) Sections: Paraffin, 2 microns Procedure: Acid washed glassware must be used!!!! 1. Deparaffinize and hydrate to distilled water. 2. Oxidize in Periodic Acid 0.5% (F-396-1) 11 minutes. Wash in chloride-free water. 3. Prepare Methenamine Silver solution by mixing: 42.5 ml Methenamine 3% (F-396-2), 2.5 ml Silver Nitrate, 5% (F-396-3) and 12.0 ml Borate Buffer, pH 8.2 (F-396-4). 4. Place slides in the solution and the entire jar in a water bath at 70?C for approx. 60-75 minutes. Check under microscope when slides appear medium brown microscopically. Every 10 minutes, once the medium brown color has been established, rinse a slide in 70?C, chloride free water and check under a microscope. Rinse again in hot water and return to the hot staining solution. As the staining time approaches the end point, check the slides, as above, every 1-2 minutes. The entire procedure must be performed quickly to prevent an uneven staining of the tissues. The slides should exhibit a brownish- yellow background, intense black reticulum fibers, and black basement membranes. If the slides become oversaturated, i.e. too black, destain in a dilute Potassium Ferricyanide Solution (F-396-11) for one or two dips. 5. Rinse well in distilled water. Tone in Gold Chloride 0.2% (F-396-5), 1 minute. If sections are overtoned place in Sodium Metabisulfite, 3% (F-396-12) for 1-3 minutes. Rinse well in distilled water. 6. Sodium Thiosulfate 3% (F-396-9), 1-2 miutes. Wash in running tap water, 10 minutes. Rinse well in distilled water. 7. Stain in Harris' Hematoxylin (F-396-6) containing 2-4ml of Glacial Acetic Acid per 100 ml for 5-15 minutes. Wash in water. 8. Differentiate in Acid Alcohol 1% (F-396-13) until the sections turn red. 9. Blue section in Ammonia Water, 0.3% (F-396-14). Wash thoroughly. 10. Counterstain in Eosin Y, 1%, Alcoholic Solution (F-396-7). 11. Dehydrate in 95% alcohol, absolute alcohol and clear in xylene 3 changes each. Mount. Stain Results: Basement membranes, reticulum fibers: Black Nuclei: Blue Cytoplasm, collagen, connective tissue: Pink-orange References: Jones, D.B., Amer.J.Path. 27:99 (1951). AFIP Manual of Histolocical Staining Methods, 3rd ed., Ed. L. Luna: NY: McGraw-Hill Publications, c. 1968, p. 97." ~~~~~ It became apparent that something went wrong during Step 4 when the slides were in the glass container (not a coplin jar - we have ten slides that we need to stain so we're using a rectangular glass container that holds ten slides on their sides - it does require a metal handle to move, but the handle is flexible and easy to remove after the glass slide rack has been transferred between containers) of silver solution in the water bath because there was lots of precipitate on the slides and floating on the surface of the silver solution. In my first test, I used five test slides (extra slides that we cut from the same blocks that were submitted to us). I deparaffinized them in coplin jars (moving them with plastic forceps) and hydrated them to deionized water. I transferred the slides to a glass slide rack that holds ten slides on their sides, added five blank slides that were rinsed in deionized water (so that the displacement of reagents would be equivalent to when we stain our ten "real" slides after testing is complete), and completed Step 2. I don't recall exactly how long the glass container of silver solution and the glass container of deionized water had been heating up in the water bath, but I would estimate ~15-30 minutes. The thermometer said that the water in the bath (not inside the containers) reached ~60-65 degrees Celsius. The silver solution was clear and colorless when I made it up, but by the time I put the slides into the warm silver solution, the solution was beginning to turn a light brown color (though it was still clear and I did not see any precipitate floating around). I removed the metal handle of the glass slide rack after the rack was transferred into the silver solution, but the metal handle did dip into the silver solution briefly. At some point, I noticed precipitate floating around of the surface of the silver solution. After ~80 minutes, I used plastic forceps to remove one test slide from the warm silver solution, dipped it several times into the warm deionized water to rinse it, and wiped off the back of the slide with gauze. The amount of precipitate was so extreme that the gauze did nearly nothing. I showed the slide to one of our pathologists and they could hardly see beyond the precipitate, but said that they couldn't see any staining of the structure that they were looking for (I forget exactly what it was, but I know it's supposed to turn black). In my second test (to see if the metal holder was the problem) that I performed immediately after the first test, I used one test slide. I deparaffinized it in the same coplin jars as before (moving it with plastic forceps) and hydrated it to deionized water. I used new glass containers for the periodic acid and deionized water rinse in Step 2, for making the silver solution in Step 3, and for the warm silver solution and warm deionized water in Step 4. I used plastic forceps to move the slide into the periodic acid, and propped it up in the container so that no glass rack or metal handle was used at all. I used plastic forceps to transfer the slide to the deionized water rinse, and dunked it several times and swished the slide around a bit. I used plastic forceps to transfer the slide into the warm(-ish) silver solution and propped it up against the side again. After approximately 20 minutes, I saw precipitate floating around, and I used plastic forceps to remove the slide from the silver solution. I dipped the slide into the warm(-ish) deionized water several times, and saw that the precipitate was again covering the slide and the tissue so I stopped there for the day. We purchased all of the reagents listed in the above procedure from Rowley Biochemical (except for the Glacial Acetic Acid mentioned in Step 7, but I didn't even get that far). Questions: 1. Could this indicate that the acid-washing was not done correctly? I made up a ~1% Hydrochloric Acid solution (with deionized water) and filled a plastic bin with the solution (I rinsed the bin with deionized water first). I then submerged all glassware (in several batches) for at least five minutes, then rinsed well with deionized water (not by filling a bin - I just used the hose of deionized water in our lab sink and poured it over the glassware) and left them to air-dry overnight. 2. Are using acid-washed glassware and avoiding metal even necessary precautions after the sodium thiosulphate in Step 6? I read that sodium thiosulphate "stops the reaction," and the procedure stops specifically saying to use deionized water after Step 6 and starts saying to use just "water" or "tap water." My lab refers to our waters as either "tap" or "deionized," so I'm assuming that using my deionized water is fine when the procedure calls for "distilled" or "dechlorinated." I don't even know enough to ask more questions, but I'm sure many more will arise after I test the stain again next week, so I welcome any and all advice about silver stains, acid-cleaning glassware, and literally anything else... Thank you!!! Jordan H. University of Michigan Ann Arbor, MI ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues From mward at wakehealth.edu Thu Sep 23 14:55:42 2021 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Thu, 23 Sep 2021 19:55:42 +0000 Subject: [Histonet] Bone marrow clot IHC tissue sections washing Message-ID: It was brought to my attention that we had significant washing on 3 of 8 bone marrow clot sections the other day; this is not the first time so we would like to get to the bottom of this. We use positively charged slides and all 8 cases were cut and run the same morning but allowed to air dry and then bake at 60C for 20 minutes before being run on our Bond 3 stainer. Has anyone out there experienced this type of problem and if so, what were your solutions? The repeat of the 3 cases today showed similar washing of tissue. This hasn't just started but has occurred periodically but the pathologists have tried to live with it and usually we can finally get enough tissue to stay on after 1-2 attempts. Suggestions include cutting and drying the slides overnight and/or going to a gelatinated slide versus a sialylated slide. We have been using this particular brand of positive charged slide with good results for several years and rarely have issues with other tissue types unless they are particularly bloody. Thoughts or suggestions are greatly appreciated. Martha Ward MT(ASCP) QIHC Atrium Health Wake Forest Baptist From regan.fulton at gmail.com Thu Sep 23 15:16:27 2021 From: regan.fulton at gmail.com (Regan Fulton) Date: Thu, 23 Sep 2021 13:16:27 -0700 Subject: [Histonet] Bone marrow clot IHC tissue sections washing In-Reply-To: References: Message-ID: Martha, We reported our study of 15 brands of adhesive slides for "wash off" and found little difference among the different slides when well-fixed cell culture material was examined. On the other hand, poorly-fixed breast cancer tissues did appear to adhere more strongly to some slides than others (TOMO being among the best). Additional factors need to be considered, though, and I note that your baking procedure is different from what is recommended by many slide vendors. In general, baking at 60-65 deg C for 1 hour is said to be optimal, although we did not examine that parameter specifically. Please see our poster at https://www.arrayscience.com/publications#Posters Best regards, Regan Regan Fulton, M.D., Ph.D. CEO and Co-Founder Array Science, LLC 475 Gate 5 Road, #100 Sausalito, CA 94965 (415) 577-7360 email: fulton at arrayscience.com www.arrayscience.com On Thu, Sep 23, 2021 at 1:02 PM Martha Ward-Pathology via Histonet < histonet at lists.utsouthwestern.edu> wrote: > It was brought to my attention that we had significant washing on 3 of 8 > bone marrow clot sections the other day; this is not the first time so we > would like to get to the bottom of this. We use positively charged slides > and all 8 cases were cut and run the same morning but allowed to air dry > and then bake at 60C for 20 minutes before being run on our Bond 3 > stainer. Has anyone out there experienced this type of problem and if so, > what were your solutions? The repeat of the 3 cases today showed similar > washing of tissue. > > This hasn't just started but has occurred periodically but the > pathologists have tried to live with it and usually we can finally get > enough tissue to stay on after 1-2 attempts. Suggestions include cutting > and drying the slides overnight and/or going to a gelatinated slide versus > a sialylated slide. We have been using this particular brand of positive > charged slide with good results for several years and rarely have issues > with other tissue types unless they are particularly bloody. > > Thoughts or suggestions are greatly appreciated. > > > Martha Ward MT(ASCP) QIHC > Atrium Health Wake Forest Baptist > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From llewllew at shaw.ca Thu Sep 23 16:47:24 2021 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Thu, 23 Sep 2021 14:47:24 -0700 Subject: [Histonet] Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions In-Reply-To: <48bd31f00b074079baca10d963e1bc2f@med.umich.edu> References: <48bd31f00b074079baca10d963e1bc2f@med.umich.edu> Message-ID: <9e1fe5b8-be5e-ae7f-ca29-e57dac959071@shaw.ca> Hi, Try the method given in StainsFile at: http://stainsfile.info/stain/metallic/jones.htm Bryan Llewellyn Hood, Jordan via Histonet wrote: > Hello, > > I'm new to histology (and new to histonet), and I work in a small histology lab specializing in animal tissues that receives requests/submissions from researchers. I tried (and failed) to perform a Jones' Methenamine Silver stain on a client's submission of pig kidneys (formalin-fixed, paraffin-embedded, cut at 2.5 microns), and I need some help troubleshooting this stain since my co-workers are stumped, too. I used the following procedure from Rowley Biochemical: > > > ~~~~~ > "Fixation: 10% Buffered Neutral Formalin (F-113) or Bouin's Solution (F-40) or Zenker's (F-155) > > Sections: Paraffin, 2 microns > > Procedure: Acid washed glassware must be used!!!! > 1. Deparaffinize and hydrate to distilled water. > 2. Oxidize in Periodic Acid 0.5% (F-396-1) 11 minutes. Wash in chloride-free water. > 3. Prepare Methenamine Silver solution by mixing: 42.5 ml Methenamine 3% (F-396-2), 2.5 ml Silver Nitrate, 5% (F-396-3) and 12.0 ml Borate Buffer, pH 8.2 (F-396-4). > 4. Place slides in the solution and the entire jar in a water bath at 70?C for approx. 60-75 minutes. Check under microscope when slides appear medium brown microscopically. Every 10 minutes, once the medium brown color has been established, rinse a slide in 70?C, chloride free water and check under a microscope. Rinse again in hot water and return to the hot staining solution. As the staining time approaches the end point, check the slides, as above, every 1-2 minutes. The entire procedure must be performed quickly to prevent an uneven staining of the tissues. The slides should exhibit a brownish- yellow background, intense black reticulum fibers, and black basement membranes. If the slides become oversaturated, i.e. too black, destain in a dilute Potassium Ferricyanide Solution (F-396-11) for one or two dips. > 5. Rinse well in distilled water. Tone in Gold Chloride 0.2% (F-396-5), 1 minute. If sections are overtoned place in Sodium Metabisulfite, 3% (F-396-12) for 1-3 minutes. Rinse well in distilled water. > 6. Sodium Thiosulfate 3% (F-396-9), 1-2 miutes. Wash in running tap water, 10 minutes. Rinse well in distilled water. > 7. Stain in Harris' Hematoxylin (F-396-6) containing 2-4ml of Glacial Acetic Acid per 100 ml for 5-15 minutes. Wash in water. > 8. Differentiate in Acid Alcohol 1% (F-396-13) until the sections turn red. > 9. Blue section in Ammonia Water, 0.3% (F-396-14). Wash thoroughly. > 10. Counterstain in Eosin Y, 1%, Alcoholic Solution (F-396-7). > 11. Dehydrate in 95% alcohol, absolute alcohol and clear in xylene 3 changes each. Mount. > > Stain Results: > Basement membranes, reticulum fibers: Black > Nuclei: Blue > Cytoplasm, collagen, connective tissue: Pink-orange > > References: Jones, D.B., Amer.J.Path. 27:99 (1951). AFIP Manual of Histolocical Staining Methods, 3rd ed., Ed. L. Luna: NY: McGraw-Hill Publications, c. 1968, p. 97." > ~~~~~ > > > It became apparent that something went wrong during Step 4 when the slides were in the glass container (not a coplin jar - we have ten slides that we need to stain so we're using a rectangular glass container that holds ten slides on their sides - it does require a metal handle to move, but the handle is flexible and easy to remove after the glass slide rack has been transferred between containers) of silver solution in the water bath because there was lots of precipitate on the slides and floating on the surface of the silver solution. > > In my first test, I used five test slides (extra slides that we cut from the same blocks that were submitted to us). I deparaffinized them in coplin jars (moving them with plastic forceps) and hydrated them to deionized water. I transferred the slides to a glass slide rack that holds ten slides on their sides, added five blank slides that were rinsed in deionized water (so that the displacement of reagents would be equivalent to when we stain our ten "real" slides after testing is complete), and completed Step 2. I don't recall exactly how long the glass container of silver solution and the glass container of deionized water had been heating up in the water bath, but I would estimate ~15-30 minutes. The thermometer said that the water in the bath (not inside the containers) reached ~60-65 degrees Celsius. The silver solution was clear and colorless when I made it up, but by the time I put the slides into the warm silver solution, the solution was beginning to turn a light brown color (though it was still clear and I did not see any precipitate floating around). I removed the metal handle of the glass slide rack after the rack was transferred into the silver solution, but the metal handle did dip into the silver solution briefly. At some point, I noticed precipitate floating around of the surface of the silver solution. After ~80 minutes, I used plastic forceps to remove one test slide from the warm silver solution, dipped it several times into the warm deionized water to rinse it, and wiped off the back of the slide with gauze. The amount of precipitate was so extreme that the gauze did nearly nothing. I showed the slide to one of our pathologists and they could hardly see beyond the precipitate, but said that they couldn't see any staining of the structure that they were looking for (I forget exactly what it was, but I know it's supposed to turn black). > > In my second test (to see if the metal holder was the problem) that I performed immediately after the first test, I used one test slide. I deparaffinized it in the same coplin jars as before (moving it with plastic forceps) and hydrated it to deionized water. I used new glass containers for the periodic acid and deionized water rinse in Step 2, for making the silver solution in Step 3, and for the warm silver solution and warm deionized water in Step 4. I used plastic forceps to move the slide into the periodic acid, and propped it up in the container so that no glass rack or metal handle was used at all. I used plastic forceps to transfer the slide to the deionized water rinse, and dunked it several times and swished the slide around a bit. I used plastic forceps to transfer the slide into the warm(-ish) silver solution and propped it up against the side again. After approximately 20 minutes, I saw precipitate floating around, and I used plastic forceps to remove the slide from the silver solution. I dipped the slide into the warm(-ish) deionized water several times, and saw that the precipitate was again covering the slide and the tissue so I stopped there for the day. > > We purchased all of the reagents listed in the above procedure from Rowley Biochemical (except for the Glacial Acetic Acid mentioned in Step 7, but I didn't even get that far). > > Questions: > > 1. Could this indicate that the acid-washing was not done correctly? I made up a ~1% Hydrochloric Acid solution (with deionized water) and filled a plastic bin with the solution (I rinsed the bin with deionized water first). I then submerged all glassware (in several batches) for at least five minutes, then rinsed well with deionized water (not by filling a bin - I just used the hose of deionized water in our lab sink and poured it over the glassware) and left them to air-dry overnight. > > 2. Are using acid-washed glassware and avoiding metal even necessary precautions after the sodium thiosulphate in Step 6? I read that sodium thiosulphate "stops the reaction," and the procedure stops specifically saying to use deionized water after Step 6 and starts saying to use just "water" or "tap water." My lab refers to our waters as either "tap" or "deionized," so I'm assuming that using my deionized water is fine when the procedure calls for "distilled" or "dechlorinated." > > I don't even know enough to ask more questions, but I'm sure many more will arise after I test the stain again next week, so I welcome any and all advice about silver stains, acid-cleaning glassware, and literally anything else... > > Thank you!!! > > Jordan H. > University of Michigan > Ann Arbor, MI > ********************************************************** > Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tony.henwood at health.nsw.gov.au Thu Sep 23 18:13:40 2021 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 23 Sep 2021 23:13:40 +0000 Subject: [Histonet] Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions In-Reply-To: <9e1fe5b8-be5e-ae7f-ca29-e57dac959071@shaw.ca> References: <48bd31f00b074079baca10d963e1bc2f@med.umich.edu> <9e1fe5b8-be5e-ae7f-ca29-e57dac959071@shaw.ca> Message-ID: <5a34030c402f4f4b97dec87e4856d6f6@SVDCMBX-MEX024.nswhealth.net> I agree with Bryan, The introduction of thiosemicarbazide before the silver step improves the staining immensely. I would also look at the periodic acid. Is it too dilute, though 0.5% should work? I usually cover this by using a 1% solution for 20 minutes. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Bryan Llewellyn via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 24 September 2021 7:47 AM To: Jordan ; Histonet Subject: Re: [Histonet] Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions Hi, Try the method given in StainsFile at: http://stainsfile.info/stain/metallic/jones.htm Bryan Llewellyn Hood, Jordan via Histonet wrote: > Hello, > > I'm new to histology (and new to histonet), and I work in a small histology lab specializing in animal tissues that receives requests/submissions from researchers. I tried (and failed) to perform a Jones' Methenamine Silver stain on a client's submission of pig kidneys (formalin-fixed, paraffin-embedded, cut at 2.5 microns), and I need some help troubleshooting this stain since my co-workers are stumped, too. I used the following procedure from Rowley Biochemical: > > > ~~~~~ > "Fixation: 10% Buffered Neutral Formalin (F-113) or Bouin's Solution > (F-40) or Zenker's (F-155) > > Sections: Paraffin, 2 microns > > Procedure: Acid washed glassware must be used!!!! > 1. Deparaffinize and hydrate to distilled water. > 2. Oxidize in Periodic Acid 0.5% (F-396-1) 11 minutes. Wash in chloride-free water. > 3. Prepare Methenamine Silver solution by mixing: 42.5 ml Methenamine 3% (F-396-2), 2.5 ml Silver Nitrate, 5% (F-396-3) and 12.0 ml Borate Buffer, pH 8.2 (F-396-4). > 4. Place slides in the solution and the entire jar in a water bath at 70?C for approx. 60-75 minutes. Check under microscope when slides appear medium brown microscopically. Every 10 minutes, once the medium brown color has been established, rinse a slide in 70?C, chloride free water and check under a microscope. Rinse again in hot water and return to the hot staining solution. As the staining time approaches the end point, check the slides, as above, every 1-2 minutes. The entire procedure must be performed quickly to prevent an uneven staining of the tissues. The slides should exhibit a brownish- yellow background, intense black reticulum fibers, and black basement membranes. If the slides become oversaturated, i.e. too black, destain in a dilute Potassium Ferricyanide Solution (F-396-11) for one or two dips. > 5. Rinse well in distilled water. Tone in Gold Chloride 0.2% (F-396-5), 1 minute. If sections are overtoned place in Sodium Metabisulfite, 3% (F-396-12) for 1-3 minutes. Rinse well in distilled water. > 6. Sodium Thiosulfate 3% (F-396-9), 1-2 miutes. Wash in running tap water, 10 minutes. Rinse well in distilled water. > 7. Stain in Harris' Hematoxylin (F-396-6) containing 2-4ml of Glacial Acetic Acid per 100 ml for 5-15 minutes. Wash in water. > 8. Differentiate in Acid Alcohol 1% (F-396-13) until the sections turn red. > 9. Blue section in Ammonia Water, 0.3% (F-396-14). Wash thoroughly. > 10. Counterstain in Eosin Y, 1%, Alcoholic Solution (F-396-7). > 11. Dehydrate in 95% alcohol, absolute alcohol and clear in xylene 3 changes each. Mount. > > Stain Results: > Basement membranes, reticulum fibers: Black > Nuclei: Blue > Cytoplasm, collagen, connective tissue: Pink-orange > > References: Jones, D.B., Amer.J.Path. 27:99 (1951). AFIP Manual of Histolocical Staining Methods, 3rd ed., Ed. L. Luna: NY: McGraw-Hill Publications, c. 1968, p. 97." > ~~~~~ > > > It became apparent that something went wrong during Step 4 when the slides were in the glass container (not a coplin jar - we have ten slides that we need to stain so we're using a rectangular glass container that holds ten slides on their sides - it does require a metal handle to move, but the handle is flexible and easy to remove after the glass slide rack has been transferred between containers) of silver solution in the water bath because there was lots of precipitate on the slides and floating on the surface of the silver solution. > > In my first test, I used five test slides (extra slides that we cut from the same blocks that were submitted to us). I deparaffinized them in coplin jars (moving them with plastic forceps) and hydrated them to deionized water. I transferred the slides to a glass slide rack that holds ten slides on their sides, added five blank slides that were rinsed in deionized water (so that the displacement of reagents would be equivalent to when we stain our ten "real" slides after testing is complete), and completed Step 2. I don't recall exactly how long the glass container of silver solution and the glass container of deionized water had been heating up in the water bath, but I would estimate ~15-30 minutes. The thermometer said that the water in the bath (not inside the containers) reached ~60-65 degrees Celsius. The silver solution was clear and colorless when I made it up, but by the time I put the slides into the warm silver solution, the solution was beginning to turn a light brown color (though it was still clear and I did not see any precipitate floating around). I removed the metal handle of the glass slide rack after the rack was transferred into the silver solution, but the metal handle did dip into the silver solution briefly. At some point, I noticed precipitate floating around of the surface of the silver solution. After ~80 minutes, I used plastic forceps to remove one test slide from the warm silver solution, dipped it several times into the warm deionized water to rinse it, and wiped off the back of the slide with gauze. The amount of precipitate was so extreme that the gauze did nearly nothing. I showed the slide to one of our pathologists and they could hardly see beyond the precipitate, but said that they couldn't see any staining of the structure that they were looking for (I forget exactly what it was, but I know it's supposed to turn black). > > In my second test (to see if the metal holder was the problem) that I performed immediately after the first test, I used one test slide. I deparaffinized it in the same coplin jars as before (moving it with plastic forceps) and hydrated it to deionized water. I used new glass containers for the periodic acid and deionized water rinse in Step 2, for making the silver solution in Step 3, and for the warm silver solution and warm deionized water in Step 4. I used plastic forceps to move the slide into the periodic acid, and propped it up in the container so that no glass rack or metal handle was used at all. I used plastic forceps to transfer the slide to the deionized water rinse, and dunked it several times and swished the slide around a bit. I used plastic forceps to transfer the slide into the warm(-ish) silver solution and propped it up against the side again. After approximately 20 minutes, I saw precipitate floating around, and I used plastic forceps to remove the slide from the silver solution. I dipped the slide into the warm(-ish) deionized water several times, and saw that the precipitate was again covering the slide and the tissue so I stopped there for the day. > > We purchased all of the reagents listed in the above procedure from Rowley Biochemical (except for the Glacial Acetic Acid mentioned in Step 7, but I didn't even get that far). > > Questions: > > 1. Could this indicate that the acid-washing was not done correctly? I made up a ~1% Hydrochloric Acid solution (with deionized water) and filled a plastic bin with the solution (I rinsed the bin with deionized water first). I then submerged all glassware (in several batches) for at least five minutes, then rinsed well with deionized water (not by filling a bin - I just used the hose of deionized water in our lab sink and poured it over the glassware) and left them to air-dry overnight. > > 2. Are using acid-washed glassware and avoiding metal even necessary precautions after the sodium thiosulphate in Step 6? I read that sodium thiosulphate "stops the reaction," and the procedure stops specifically saying to use deionized water after Step 6 and starts saying to use just "water" or "tap water." My lab refers to our waters as either "tap" or "deionized," so I'm assuming that using my deionized water is fine when the procedure calls for "distilled" or "dechlorinated." > > I don't even know enough to ask more questions, but I'm sure many more will arise after I test the stain again next week, so I welcome any and all advice about silver stains, acid-cleaning glassware, and literally anything else... > > Thank you!!! > > Jordan H. > University of Michigan > Ann Arbor, MI > ********************************************************** > Electronic Mail is not secure, may not be read every day, and should > not be used for urgent or sensitive issues > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From cforster at umn.edu Thu Sep 23 18:28:03 2021 From: cforster at umn.edu (Colleen Forster) Date: Thu, 23 Sep 2021 18:28:03 -0500 Subject: [Histonet] Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions In-Reply-To: <5a34030c402f4f4b97dec87e4856d6f6@SVDCMBX-MEX024.nswhealth.net> References: <48bd31f00b074079baca10d963e1bc2f@med.umich.edu> <9e1fe5b8-be5e-ae7f-ca29-e57dac959071@shaw.ca> <5a34030c402f4f4b97dec87e4856d6f6@SVDCMBX-MEX024.nswhealth.net> Message-ID: Make sure the periodic acid is made fresh EACH time you run the stain. That can also make a big difference in the stain quality. Colleen Forster HT(ASCP)QIHC On Thu, Sep 23, 2021 at 6:14 PM Tony Henwood (SCHN) via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I agree with Bryan, > > The introduction of thiosemicarbazide before the silver step improves the > staining immensely. > > I would also look at the periodic acid. Is it too dilute, though 0.5% > should work? I usually cover this by using a 1% solution for 20 minutes. > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children?s Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: Bryan Llewellyn via Histonet [mailto: > histonet at lists.utsouthwestern.edu] > Sent: Friday, 24 September 2021 7:47 AM > To: Jordan ; Histonet < > histonet at lists.utsouthwestern.edu> > Subject: Re: [Histonet] Jones' Methenamine Silver Stain for Basement > Membranes of Kidney - Issues and Questions > > Hi, > Try the method given in StainsFile at: > http://stainsfile.info/stain/metallic/jones.htm > > Bryan Llewellyn > > > Hood, Jordan via Histonet wrote: > > Hello, > > > > I'm new to histology (and new to histonet), and I work in a small > histology lab specializing in animal tissues that receives > requests/submissions from researchers. I tried (and failed) to perform a > Jones' Methenamine Silver stain on a client's submission of pig kidneys > (formalin-fixed, paraffin-embedded, cut at 2.5 microns), and I need some > help troubleshooting this stain since my co-workers are stumped, too. I > used the following procedure from Rowley Biochemical: > > > > > > ~~~~~ > > "Fixation: 10% Buffered Neutral Formalin (F-113) or Bouin's Solution > > (F-40) or Zenker's (F-155) > > > > Sections: Paraffin, 2 microns > > > > Procedure: Acid washed glassware must be used!!!! > > 1. Deparaffinize and hydrate to distilled water. > > 2. Oxidize in Periodic Acid 0.5% (F-396-1) 11 minutes. Wash in > chloride-free water. > > 3. Prepare Methenamine Silver solution by mixing: 42.5 ml Methenamine 3% > (F-396-2), 2.5 ml Silver Nitrate, 5% (F-396-3) and 12.0 ml Borate Buffer, > pH 8.2 (F-396-4). > > 4. Place slides in the solution and the entire jar in a water bath at > 70?C for approx. 60-75 minutes. Check under microscope when slides appear > medium brown microscopically. Every 10 minutes, once the medium brown color > has been established, rinse a slide in 70?C, chloride free water and check > under a microscope. Rinse again in hot water and return to the hot staining > solution. As the staining time approaches the end point, check the slides, > as above, every 1-2 minutes. The entire procedure must be performed quickly > to prevent an uneven staining of the tissues. The slides should exhibit a > brownish- yellow background, intense black reticulum fibers, and black > basement membranes. If the slides become oversaturated, i.e. too black, > destain in a dilute Potassium Ferricyanide Solution (F-396-11) for one or > two dips. > > 5. Rinse well in distilled water. Tone in Gold Chloride 0.2% (F-396-5), > 1 minute. If sections are overtoned place in Sodium Metabisulfite, 3% > (F-396-12) for 1-3 minutes. Rinse well in distilled water. > > 6. Sodium Thiosulfate 3% (F-396-9), 1-2 miutes. Wash in running tap > water, 10 minutes. Rinse well in distilled water. > > 7. Stain in Harris' Hematoxylin (F-396-6) containing 2-4ml of Glacial > Acetic Acid per 100 ml for 5-15 minutes. Wash in water. > > 8. Differentiate in Acid Alcohol 1% (F-396-13) until the sections turn > red. > > 9. Blue section in Ammonia Water, 0.3% (F-396-14). Wash thoroughly. > > 10. Counterstain in Eosin Y, 1%, Alcoholic Solution (F-396-7). > > 11. Dehydrate in 95% alcohol, absolute alcohol and clear in xylene 3 > changes each. Mount. > > > > Stain Results: > > Basement membranes, reticulum fibers: Black > > Nuclei: Blue > > Cytoplasm, collagen, connective tissue: Pink-orange > > > > References: Jones, D.B., Amer.J.Path. 27:99 (1951). AFIP Manual of > Histolocical Staining Methods, 3rd ed., Ed. L. Luna: NY: McGraw-Hill > Publications, c. 1968, p. 97." > > ~~~~~ > > > > > > It became apparent that something went wrong during Step 4 when the > slides were in the glass container (not a coplin jar - we have ten slides > that we need to stain so we're using a rectangular glass container that > holds ten slides on their sides - it does require a metal handle to move, > but the handle is flexible and easy to remove after the glass slide rack > has been transferred between containers) of silver solution in the water > bath because there was lots of precipitate on the slides and floating on > the surface of the silver solution. > > > > In my first test, I used five test slides (extra slides that we cut from > the same blocks that were submitted to us). I deparaffinized them in coplin > jars (moving them with plastic forceps) and hydrated them to deionized > water. I transferred the slides to a glass slide rack that holds ten slides > on their sides, added five blank slides that were rinsed in deionized water > (so that the displacement of reagents would be equivalent to when we stain > our ten "real" slides after testing is complete), and completed Step 2. I > don't recall exactly how long the glass container of silver solution and > the glass container of deionized water had been heating up in the water > bath, but I would estimate ~15-30 minutes. The thermometer said that the > water in the bath (not inside the containers) reached ~60-65 degrees > Celsius. The silver solution was clear and colorless when I made it up, but > by the time I put the slides into the warm silver solution, the solution > was beginning to turn a light brown color (though it was still clear and I > did not see any precipitate floating around). I removed the metal handle of > the glass slide rack after the rack was transferred into the silver > solution, but the metal handle did dip into the silver solution briefly. At > some point, I noticed precipitate floating around of the surface of the > silver solution. After ~80 minutes, I used plastic forceps to remove one > test slide from the warm silver solution, dipped it several times into the > warm deionized water to rinse it, and wiped off the back of the slide with > gauze. The amount of precipitate was so extreme that the gauze did nearly > nothing. I showed the slide to one of our pathologists and they could > hardly see beyond the precipitate, but said that they couldn't see any > staining of the structure that they were looking for (I forget exactly what > it was, but I know it's supposed to turn black). > > > > In my second test (to see if the metal holder was the problem) that I > performed immediately after the first test, I used one test slide. I > deparaffinized it in the same coplin jars as before (moving it with plastic > forceps) and hydrated it to deionized water. I used new glass containers > for the periodic acid and deionized water rinse in Step 2, for making the > silver solution in Step 3, and for the warm silver solution and warm > deionized water in Step 4. I used plastic forceps to move the slide into > the periodic acid, and propped it up in the container so that no glass rack > or metal handle was used at all. I used plastic forceps to transfer the > slide to the deionized water rinse, and dunked it several times and swished > the slide around a bit. I used plastic forceps to transfer the slide into > the warm(-ish) silver solution and propped it up against the side again. > After approximately 20 minutes, I saw precipitate floating around, and I > used plastic forceps to remove the slide from the silver solution. I dipped > the slide into the warm(-ish) deionized water several times, and saw that > the precipitate was again covering the slide and the tissue so I stopped > there for the day. > > > > We purchased all of the reagents listed in the above procedure from > Rowley Biochemical (except for the Glacial Acetic Acid mentioned in Step 7, > but I didn't even get that far). > > > > Questions: > > > > 1. Could this indicate that the acid-washing was not done correctly? I > made up a ~1% Hydrochloric Acid solution (with deionized water) and filled > a plastic bin with the solution (I rinsed the bin with deionized water > first). I then submerged all glassware (in several batches) for at least > five minutes, then rinsed well with deionized water (not by filling a bin - > I just used the hose of deionized water in our lab sink and poured it over > the glassware) and left them to air-dry overnight. > > > > 2. Are using acid-washed glassware and avoiding metal even necessary > precautions after the sodium thiosulphate in Step 6? I read that sodium > thiosulphate "stops the reaction," and the procedure stops specifically > saying to use deionized water after Step 6 and starts saying to use just > "water" or "tap water." My lab refers to our waters as either "tap" or > "deionized," so I'm assuming that using my deionized water is fine when the > procedure calls for "distilled" or "dechlorinated." > > > > I don't even know enough to ask more questions, but I'm sure many more > will arise after I test the stain again next week, so I welcome any and all > advice about silver stains, acid-cleaning glassware, and literally anything > else... > > > > Thank you!!! > > > > Jordan H. > > University of Michigan > > Ann Arbor, MI > > ********************************************************** > > Electronic Mail is not secure, may not be read every day, and should > > not be used for urgent or sensitive issues > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain > confidential information. If you are not the intended recipient, please > delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and > are not necessarily the views of NSW Health or any of its entities. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From amosbrooks at gmail.com Sat Sep 25 07:02:43 2021 From: amosbrooks at gmail.com (Amos Brooks) Date: Sat, 25 Sep 2021 08:02:43 -0400 Subject: [Histonet] Jones problems Message-ID: Hi, When I first did this stain, I had really light staining of the GBM. StainsFile has a note in the procedure that describes thiosemicarbizide. Being a complete nerd, I wanted to see another source as well. Here is a link to an article where it is used... https://pubmed.ncbi.nlm.nih.gov/2482996/ I also make these chemicals freshly each time. I accomplish this by pre-weighing the solids sufficient for a 50ml volume and storing it in an eppendorf tube. When you need it, pop the tube into the requirement volume of water and you're golden. I do this because in addition to having good freshly made solutions, I found the thiosemicarbizide goes bad rather quickly after use. TSC is the lynch pin for the procedure. The silver on it's own does not react very strongly with the aldehydes that are reduced by the periodic acid without the TSC to facilitate the precipitation. I hope this helps, Amos Brooks From kathryn.perkinson at duke.edu Tue Sep 28 06:51:15 2021 From: kathryn.perkinson at duke.edu (Kathryn Perkinson) Date: Tue, 28 Sep 2021 11:51:15 +0000 Subject: [Histonet] Histonet Digest, Vol 214, Issue 14 In-Reply-To: References: Message-ID: LABORATORY SUPERVISOR POSITION AT DUKE UNIVERSITY MEDICAL CENTER DURHAM NC We have an opening for a Laboratory Supervisor position in the Division of Anatomic Pathology and Digital Analytics. The position requires a BS degree in biological science, certification as HTL or HT, and 5 years of Histology experience. Experience preferred but not required includes supervisory, IHC, FISH, or digital pathology. Contact Kathryn.perkinson at duke.edu. Thank you Kathy Kathryn R. Perkinson, BS, HTL(ASCP), CSSGB Manager, Division of Anatomic Pathology and Digital Analytics Box 3712 Room 4710 Duke South Clinic Building, Yellow Zone Duke University Health System Durham, NC 27710 Phone: 919-684-5822 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Friday, September 24, 2021 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 214, Issue 14 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrVmY_FPmCDYnGHGszj4cR01z2-BarQ$ or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Multiple H&E stainer maintenance (Bacon, Charles) 2. Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions (Hood, Jordan) 3. Bone marrow clot IHC tissue sections washing (Martha Ward-Pathology) 4. Re: Bone marrow clot IHC tissue sections washing (Regan Fulton) 5. Re: Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions (Bryan Llewellyn) 6. Re: Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions (Tony Henwood (SCHN)) 7. Re: Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions (Colleen Forster) ---------------------------------------------------------------------- Message: 1 Date: Thu, 23 Sep 2021 17:17:42 +0000 From: "Bacon, Charles" To: "Hanson, Leslie I :LLS Lab" , "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] Multiple H&E stainer maintenance Message-ID: <2211e9fd3b204d3790182cdbf0026398 at ZXSWEXCHMXPR06.bhs.org> Content-Type: text/plain; charset="iso-8859-1" Hi Leslie, I have attached our current log. You can augment it based off the solutions you are using and how many shifts you have. We have two stainers and each has their own log. Our stainers are connected to running water thus the drain lines that need cleaning. If you have any questions reach out anytime. Good luck! Best, Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786? Fax: 413-322-4790 Charles.Bacon at baystatehealth.org -----Original Message----- From: Hanson, Leslie I :LLS Lab [mailto:LIHANSON at lhs.org] Sent: Wednesday, September 22, 2021 2:29 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Multiple H&E stainer maintenance Hi all, We recently acquired a second H&E stainer and are working up a maintenance log. Looking for input from others who have already tackled this issue! * Do you have a log for EACH instrument or one combined log? * Do you have a space for listing reagent lots? * What is your rotation schedule for changing reagents? * Other tips and tricks are always appreciated! Thanks! Leslie Hanson, HT(ASCP) Tech Specialist IHC / Pathology Phone: (503)944-7923 lihanson at lhs.org ---------------------------------------------------------------------- Please view our annual report at https://urldefense.com/v3/__http://www.bhannualreport.org__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrVmY_FPmCDYnGHGszj4cR01_x1WikQ$ CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at 413-794-0000 and destroy all copies of this communication and any attachments. For further information regarding Baystate Health's privacy policy, please visit our Internet site at https://urldefense.com/v3/__https://www.baystatehealth.org__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrVmY_FPmCDYnGHGszj4cR01w9eeq74$ . ------------------------------ Message: 2 Date: Thu, 23 Sep 2021 19:49:52 +0000 From: "Hood, Jordan" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions Message-ID: <48bd31f00b074079baca10d963e1bc2f at med.umich.edu> Content-Type: text/plain; charset="iso-8859-1" Hello, I'm new to histology (and new to histonet), and I work in a small histology lab specializing in animal tissues that receives requests/submissions from researchers. I tried (and failed) to perform a Jones' Methenamine Silver stain on a client's submission of pig kidneys (formalin-fixed, paraffin-embedded, cut at 2.5 microns), and I need some help troubleshooting this stain since my co-workers are stumped, too. I used the following procedure from Rowley Biochemical: ~~~~~ "Fixation: 10% Buffered Neutral Formalin (F-113) or Bouin's Solution (F-40) or Zenker's (F-155) Sections: Paraffin, 2 microns Procedure: Acid washed glassware must be used!!!! 1. Deparaffinize and hydrate to distilled water. 2. Oxidize in Periodic Acid 0.5% (F-396-1) 11 minutes. Wash in chloride-free water. 3. Prepare Methenamine Silver solution by mixing: 42.5 ml Methenamine 3% (F-396-2), 2.5 ml Silver Nitrate, 5% (F-396-3) and 12.0 ml Borate Buffer, pH 8.2 (F-396-4). 4. Place slides in the solution and the entire jar in a water bath at 70?C for approx. 60-75 minutes. Check under microscope when slides appear medium brown microscopically. Every 10 minutes, once the medium brown color has been established, rinse a slide in 70?C, chloride free water and check under a microscope. Rinse again in hot water and return to the hot staining solution. As the staining time approaches the end point, check the slides, as above, every 1-2 minutes. The entire procedure must be performed quickly to prevent an uneven staining of the tissues. The slides should exhibit a brownish- yellow background, intense black reticulum fibers, and black basement membranes. If the slides become oversaturated, i.e. too black, destain in a dilute Potassium Ferricyanide Solution (F-396-11) for one or two dips. 5. Rinse well in distilled water. Tone in Gold Chloride 0.2% (F-396-5), 1 minute. If sections are overtoned place in Sodium Metabisulfite, 3% (F-396-12) for 1-3 minutes. Rinse well in distilled water. 6. Sodium Thiosulfate 3% (F-396-9), 1-2 miutes. Wash in running tap water, 10 minutes. Rinse well in distilled water. 7. Stain in Harris' Hematoxylin (F-396-6) containing 2-4ml of Glacial Acetic Acid per 100 ml for 5-15 minutes. Wash in water. 8. Differentiate in Acid Alcohol 1% (F-396-13) until the sections turn red. 9. Blue section in Ammonia Water, 0.3% (F-396-14). Wash thoroughly. 10. Counterstain in Eosin Y, 1%, Alcoholic Solution (F-396-7). 11. Dehydrate in 95% alcohol, absolute alcohol and clear in xylene 3 changes each. Mount. Stain Results: Basement membranes, reticulum fibers: Black Nuclei: Blue Cytoplasm, collagen, connective tissue: Pink-orange References: Jones, D.B., Amer.J.Path. 27:99 (1951). AFIP Manual of Histolocical Staining Methods, 3rd ed., Ed. L. Luna: NY: McGraw-Hill Publications, c. 1968, p. 97." ~~~~~ It became apparent that something went wrong during Step 4 when the slides were in the glass container (not a coplin jar - we have ten slides that we need to stain so we're using a rectangular glass container that holds ten slides on their sides - it does require a metal handle to move, but the handle is flexible and easy to remove after the glass slide rack has been transferred between containers) of silver solution in the water bath because there was lots of precipitate on the slides and floating on the surface of the silver solution. In my first test, I used five test slides (extra slides that we cut from the same blocks that were submitted to us). I deparaffinized them in coplin jars (moving them with plastic forceps) and hydrated them to deionized water. I transferred the slides to a glass slide rack that holds ten slides on their sides, added five blank slides that were rinsed in deionized water (so that the displacement of reagents would be equivalent to when we stain our ten "real" slides after testing is complete), and completed Step 2. I don't recall exactly how long the glass container of silver solution and the glass container of deionized water had been heating up in the water bath, but I would estimate ~15-30 minutes. The thermometer said that the water in the bath (not inside the containers) reached ~60-65 degrees Celsius. The silver solution was clear and colorless when I made it up, but by the time I put the slides into the warm silver solution, the solution was beginning to turn a light brown color (though it was still clear and I did not see any precipitate floating around). I removed the metal handle of the glass slide rack after the rack was transferred into the silver solution, but the metal handle did dip into the silver solution briefly. At some point, I noticed precipitate floating around of the surface of the silver solution. After ~80 minutes, I used plastic forceps to remove one test slide from the warm silver solution, dipped it several times into the warm deionized water to rinse it, and wiped off the back of the slide with gauze. The amount of precipitate was so extreme that the gauze did nearly nothing. I showed the slide to one of our pathologists and they could hardly see beyond the precipitate, but said that they couldn't see any staining of the structure that they were looking for (I forget exactly what it was, but I know it's supposed to turn black). In my second test (to see if the metal holder was the problem) that I performed immediately after the first test, I used one test slide. I deparaffinized it in the same coplin jars as before (moving it with plastic forceps) and hydrated it to deionized water. I used new glass containers for the periodic acid and deionized water rinse in Step 2, for making the silver solution in Step 3, and for the warm silver solution and warm deionized water in Step 4. I used plastic forceps to move the slide into the periodic acid, and propped it up in the container so that no glass rack or metal handle was used at all. I used plastic forceps to transfer the slide to the deionized water rinse, and dunked it several times and swished the slide around a bit. I used plastic forceps to transfer the slide into the warm(-ish) silver solution and propped it up against the side again. After approximately 20 minutes, I saw precipitate floating around, and I used plastic forceps to remove the slide from the silver solution. I dipped the slide into the warm(-ish) deionized water several times, and saw that the precipitate was again covering the slide and the tissue so I stopped there for the day. We purchased all of the reagents listed in the above procedure from Rowley Biochemical (except for the Glacial Acetic Acid mentioned in Step 7, but I didn't even get that far). Questions: 1. Could this indicate that the acid-washing was not done correctly? I made up a ~1% Hydrochloric Acid solution (with deionized water) and filled a plastic bin with the solution (I rinsed the bin with deionized water first). I then submerged all glassware (in several batches) for at least five minutes, then rinsed well with deionized water (not by filling a bin - I just used the hose of deionized water in our lab sink and poured it over the glassware) and left them to air-dry overnight. 2. Are using acid-washed glassware and avoiding metal even necessary precautions after the sodium thiosulphate in Step 6? I read that sodium thiosulphate "stops the reaction," and the procedure stops specifically saying to use deionized water after Step 6 and starts saying to use just "water" or "tap water." My lab refers to our waters as either "tap" or "deionized," so I'm assuming that using my deionized water is fine when the procedure calls for "distilled" or "dechlorinated." I don't even know enough to ask more questions, but I'm sure many more will arise after I test the stain again next week, so I welcome any and all advice about silver stains, acid-cleaning glassware, and literally anything else... Thank you!!! Jordan H. University of Michigan Ann Arbor, MI ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues ------------------------------ Message: 3 Date: Thu, 23 Sep 2021 19:55:42 +0000 From: Martha Ward-Pathology To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Bone marrow clot IHC tissue sections washing Message-ID: Content-Type: text/plain; charset="us-ascii" It was brought to my attention that we had significant washing on 3 of 8 bone marrow clot sections the other day; this is not the first time so we would like to get to the bottom of this. We use positively charged slides and all 8 cases were cut and run the same morning but allowed to air dry and then bake at 60C for 20 minutes before being run on our Bond 3 stainer. Has anyone out there experienced this type of problem and if so, what were your solutions? The repeat of the 3 cases today showed similar washing of tissue. This hasn't just started but has occurred periodically but the pathologists have tried to live with it and usually we can finally get enough tissue to stay on after 1-2 attempts. Suggestions include cutting and drying the slides overnight and/or going to a gelatinated slide versus a sialylated slide. We have been using this particular brand of positive charged slide with good results for several years and rarely have issues with other tissue types unless they are particularly bloody. Thoughts or suggestions are greatly appreciated. Martha Ward MT(ASCP) QIHC Atrium Health Wake Forest Baptist ------------------------------ Message: 4 Date: Thu, 23 Sep 2021 13:16:27 -0700 From: Regan Fulton To: Martha Ward-Pathology , Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Bone marrow clot IHC tissue sections washing Message-ID: Content-Type: text/plain; charset="UTF-8" Martha, We reported our study of 15 brands of adhesive slides for "wash off" and found little difference among the different slides when well-fixed cell culture material was examined. On the other hand, poorly-fixed breast cancer tissues did appear to adhere more strongly to some slides than others (TOMO being among the best). Additional factors need to be considered, though, and I note that your baking procedure is different from what is recommended by many slide vendors. In general, baking at 60-65 deg C for 1 hour is said to be optimal, although we did not examine that parameter specifically. Please see our poster at https://urldefense.com/v3/__https://www.arrayscience.com/publications*Posters__;Iw!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrVmY_FPmCDYnGHGszj4cR01HGjCd-A$ Best regards, Regan Regan Fulton, M.D., Ph.D. CEO and Co-Founder Array Science, LLC 475 Gate 5 Road, #100 Sausalito, CA 94965 (415) 577-7360 email: fulton at arrayscience.com https://urldefense.com/v3/__http://www.arrayscience.com__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrVmY_FPmCDYnGHGszj4cR01q5l4Oys$ On Thu, Sep 23, 2021 at 1:02 PM Martha Ward-Pathology via Histonet < histonet at lists.utsouthwestern.edu> wrote: > It was brought to my attention that we had significant washing on 3 > of 8 bone marrow clot sections the other day; this is not the first time so we > would like to get to the bottom of this. We use positively charged slides > and all 8 cases were cut and run the same morning but allowed to air > dry and then bake at 60C for 20 minutes before being run on our Bond 3 > stainer. Has anyone out there experienced this type of problem and if so, > what were your solutions? The repeat of the 3 cases today showed similar > washing of tissue. > > This hasn't just started but has occurred periodically but the > pathologists have tried to live with it and usually we can finally get > enough tissue to stay on after 1-2 attempts. Suggestions include cutting > and drying the slides overnight and/or going to a gelatinated slide versus > a sialylated slide. We have been using this particular brand of positive > charged slide with good results for several years and rarely have > issues with other tissue types unless they are particularly bloody. > > Thoughts or suggestions are greatly appreciated. > > > Martha Ward MT(ASCP) QIHC > Atrium Health Wake Forest Baptist > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li > stinfo/histonet__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrV > mY_FPmCDYnGHGszj4cR01z2-BarQ$ > ------------------------------ Message: 5 Date: Thu, 23 Sep 2021 14:47:24 -0700 From: Bryan Llewellyn To: Jordan , Histonet Subject: Re: [Histonet] Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions Message-ID: <9e1fe5b8-be5e-ae7f-ca29-e57dac959071 at shaw.ca> Content-Type: text/plain; charset=UTF-8; format=flowed Hi, Try the method given in StainsFile at: https://urldefense.com/v3/__http://stainsfile.info/stain/metallic/jones.htm__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrVmY_FPmCDYnGHGszj4cR01CJSb57Q$ Bryan Llewellyn Hood, Jordan via Histonet wrote: > Hello, > > I'm new to histology (and new to histonet), and I work in a small histology lab specializing in animal tissues that receives requests/submissions from researchers. I tried (and failed) to perform a Jones' Methenamine Silver stain on a client's submission of pig kidneys (formalin-fixed, paraffin-embedded, cut at 2.5 microns), and I need some help troubleshooting this stain since my co-workers are stumped, too. I used the following procedure from Rowley Biochemical: > > > ~~~~~ > "Fixation: 10% Buffered Neutral Formalin (F-113) or Bouin's Solution > (F-40) or Zenker's (F-155) > > Sections: Paraffin, 2 microns > > Procedure: Acid washed glassware must be used!!!! > 1. Deparaffinize and hydrate to distilled water. > 2. Oxidize in Periodic Acid 0.5% (F-396-1) 11 minutes. Wash in chloride-free water. > 3. Prepare Methenamine Silver solution by mixing: 42.5 ml Methenamine 3% (F-396-2), 2.5 ml Silver Nitrate, 5% (F-396-3) and 12.0 ml Borate Buffer, pH 8.2 (F-396-4). > 4. Place slides in the solution and the entire jar in a water bath at 70?C for approx. 60-75 minutes. Check under microscope when slides appear medium brown microscopically. Every 10 minutes, once the medium brown color has been established, rinse a slide in 70?C, chloride free water and check under a microscope. Rinse again in hot water and return to the hot staining solution. As the staining time approaches the end point, check the slides, as above, every 1-2 minutes. The entire procedure must be performed quickly to prevent an uneven staining of the tissues. The slides should exhibit a brownish- yellow background, intense black reticulum fibers, and black basement membranes. If the slides become oversaturated, i.e. too black, destain in a dilute Potassium Ferricyanide Solution (F-396-11) for one or two dips. > 5. Rinse well in distilled water. Tone in Gold Chloride 0.2% (F-396-5), 1 minute. If sections are overtoned place in Sodium Metabisulfite, 3% (F-396-12) for 1-3 minutes. Rinse well in distilled water. > 6. Sodium Thiosulfate 3% (F-396-9), 1-2 miutes. Wash in running tap water, 10 minutes. Rinse well in distilled water. > 7. Stain in Harris' Hematoxylin (F-396-6) containing 2-4ml of Glacial Acetic Acid per 100 ml for 5-15 minutes. Wash in water. > 8. Differentiate in Acid Alcohol 1% (F-396-13) until the sections turn red. > 9. Blue section in Ammonia Water, 0.3% (F-396-14). Wash thoroughly. > 10. Counterstain in Eosin Y, 1%, Alcoholic Solution (F-396-7). > 11. Dehydrate in 95% alcohol, absolute alcohol and clear in xylene 3 changes each. Mount. > > Stain Results: > Basement membranes, reticulum fibers: Black > Nuclei: Blue > Cytoplasm, collagen, connective tissue: Pink-orange > > References: Jones, D.B., Amer.J.Path. 27:99 (1951). AFIP Manual of Histolocical Staining Methods, 3rd ed., Ed. L. Luna: NY: McGraw-Hill Publications, c. 1968, p. 97." > ~~~~~ > > > It became apparent that something went wrong during Step 4 when the slides were in the glass container (not a coplin jar - we have ten slides that we need to stain so we're using a rectangular glass container that holds ten slides on their sides - it does require a metal handle to move, but the handle is flexible and easy to remove after the glass slide rack has been transferred between containers) of silver solution in the water bath because there was lots of precipitate on the slides and floating on the surface of the silver solution. > > In my first test, I used five test slides (extra slides that we cut from the same blocks that were submitted to us). I deparaffinized them in coplin jars (moving them with plastic forceps) and hydrated them to deionized water. I transferred the slides to a glass slide rack that holds ten slides on their sides, added five blank slides that were rinsed in deionized water (so that the displacement of reagents would be equivalent to when we stain our ten "real" slides after testing is complete), and completed Step 2. I don't recall exactly how long the glass container of silver solution and the glass container of deionized water had been heating up in the water bath, but I would estimate ~15-30 minutes. The thermometer said that the water in the bath (not inside the containers) reached ~60-65 degrees Celsius. The silver solution was clear and colorless when I made it up, but by the time I put the slides into the warm silver solution, the solution was beginning to turn a light brown color (though it was still clear and I did not see any precipitate floating around). I removed the metal handle of the glass slide rack after the rack was transferred into the silver solution, but the metal handle did dip into the silver solution briefly. At some point, I noticed precipitate floating around of the surface of the silver solution. After ~80 minutes, I used plastic forceps to remove one test slide from the warm silver solution, dipped it several times into the warm deionized water to rinse it, and wiped off the back of the slide with gauze. The amount of precipitate was so extreme that the gauze did nearly nothing. I showed the slide to one of our pathologists and they could hardly see beyond the precipitate, but said that they couldn't see any staining of the structure that they were looking for (I forget exactly what it was, but I know it's supposed to turn black). > > In my second test (to see if the metal holder was the problem) that I performed immediately after the first test, I used one test slide. I deparaffinized it in the same coplin jars as before (moving it with plastic forceps) and hydrated it to deionized water. I used new glass containers for the periodic acid and deionized water rinse in Step 2, for making the silver solution in Step 3, and for the warm silver solution and warm deionized water in Step 4. I used plastic forceps to move the slide into the periodic acid, and propped it up in the container so that no glass rack or metal handle was used at all. I used plastic forceps to transfer the slide to the deionized water rinse, and dunked it several times and swished the slide around a bit. I used plastic forceps to transfer the slide into the warm(-ish) silver solution and propped it up against the side again. After approximately 20 minutes, I saw precipitate floating around, and I used plastic forceps to remove the slide from the silver solution. I dipped the slide into the warm(-ish) deionized water several times, and saw that the precipitate was again covering the slide and the tissue so I stopped there for the day. > > We purchased all of the reagents listed in the above procedure from Rowley Biochemical (except for the Glacial Acetic Acid mentioned in Step 7, but I didn't even get that far). > > Questions: > > 1. Could this indicate that the acid-washing was not done correctly? I made up a ~1% Hydrochloric Acid solution (with deionized water) and filled a plastic bin with the solution (I rinsed the bin with deionized water first). I then submerged all glassware (in several batches) for at least five minutes, then rinsed well with deionized water (not by filling a bin - I just used the hose of deionized water in our lab sink and poured it over the glassware) and left them to air-dry overnight. > > 2. Are using acid-washed glassware and avoiding metal even necessary precautions after the sodium thiosulphate in Step 6? I read that sodium thiosulphate "stops the reaction," and the procedure stops specifically saying to use deionized water after Step 6 and starts saying to use just "water" or "tap water." My lab refers to our waters as either "tap" or "deionized," so I'm assuming that using my deionized water is fine when the procedure calls for "distilled" or "dechlorinated." > > I don't even know enough to ask more questions, but I'm sure many more will arise after I test the stain again next week, so I welcome any and all advice about silver stains, acid-cleaning glassware, and literally anything else... > > Thank you!!! > > Jordan H. > University of Michigan > Ann Arbor, MI > ********************************************************** > Electronic Mail is not secure, may not be read every day, and should > not be used for urgent or sensitive issues > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li > stinfo/histonet__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrV > mY_FPmCDYnGHGszj4cR01z2-BarQ$ > ------------------------------ Message: 6 Date: Thu, 23 Sep 2021 23:13:40 +0000 From: "Tony Henwood (SCHN)" To: Bryan Llewellyn , Jordan Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions Message-ID: <5a34030c402f4f4b97dec87e4856d6f6 at SVDCMBX-MEX024.nswhealth.net> Content-Type: text/plain; charset="utf-8" I agree with Bryan, The introduction of thiosemicarbazide before the silver step improves the staining immensely. I would also look at the periodic acid. Is it too dilute, though 0.5% should work? I usually cover this by using a 1% solution for 20 minutes. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Bryan Llewellyn via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 24 September 2021 7:47 AM To: Jordan ; Histonet Subject: Re: [Histonet] Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions Hi, Try the method given in StainsFile at: https://urldefense.com/v3/__http://stainsfile.info/stain/metallic/jones.htm__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrVmY_FPmCDYnGHGszj4cR01CJSb57Q$ Bryan Llewellyn Hood, Jordan via Histonet wrote: > Hello, > > I'm new to histology (and new to histonet), and I work in a small histology lab specializing in animal tissues that receives requests/submissions from researchers. I tried (and failed) to perform a Jones' Methenamine Silver stain on a client's submission of pig kidneys (formalin-fixed, paraffin-embedded, cut at 2.5 microns), and I need some help troubleshooting this stain since my co-workers are stumped, too. I used the following procedure from Rowley Biochemical: > > > ~~~~~ > "Fixation: 10% Buffered Neutral Formalin (F-113) or Bouin's Solution > (F-40) or Zenker's (F-155) > > Sections: Paraffin, 2 microns > > Procedure: Acid washed glassware must be used!!!! > 1. Deparaffinize and hydrate to distilled water. > 2. Oxidize in Periodic Acid 0.5% (F-396-1) 11 minutes. Wash in chloride-free water. > 3. Prepare Methenamine Silver solution by mixing: 42.5 ml Methenamine 3% (F-396-2), 2.5 ml Silver Nitrate, 5% (F-396-3) and 12.0 ml Borate Buffer, pH 8.2 (F-396-4). > 4. Place slides in the solution and the entire jar in a water bath at 70?C for approx. 60-75 minutes. Check under microscope when slides appear medium brown microscopically. Every 10 minutes, once the medium brown color has been established, rinse a slide in 70?C, chloride free water and check under a microscope. Rinse again in hot water and return to the hot staining solution. As the staining time approaches the end point, check the slides, as above, every 1-2 minutes. The entire procedure must be performed quickly to prevent an uneven staining of the tissues. The slides should exhibit a brownish- yellow background, intense black reticulum fibers, and black basement membranes. If the slides become oversaturated, i.e. too black, destain in a dilute Potassium Ferricyanide Solution (F-396-11) for one or two dips. > 5. Rinse well in distilled water. Tone in Gold Chloride 0.2% (F-396-5), 1 minute. If sections are overtoned place in Sodium Metabisulfite, 3% (F-396-12) for 1-3 minutes. Rinse well in distilled water. > 6. Sodium Thiosulfate 3% (F-396-9), 1-2 miutes. Wash in running tap water, 10 minutes. Rinse well in distilled water. > 7. Stain in Harris' Hematoxylin (F-396-6) containing 2-4ml of Glacial Acetic Acid per 100 ml for 5-15 minutes. Wash in water. > 8. Differentiate in Acid Alcohol 1% (F-396-13) until the sections turn red. > 9. Blue section in Ammonia Water, 0.3% (F-396-14). Wash thoroughly. > 10. Counterstain in Eosin Y, 1%, Alcoholic Solution (F-396-7). > 11. Dehydrate in 95% alcohol, absolute alcohol and clear in xylene 3 changes each. Mount. > > Stain Results: > Basement membranes, reticulum fibers: Black > Nuclei: Blue > Cytoplasm, collagen, connective tissue: Pink-orange > > References: Jones, D.B., Amer.J.Path. 27:99 (1951). AFIP Manual of Histolocical Staining Methods, 3rd ed., Ed. L. Luna: NY: McGraw-Hill Publications, c. 1968, p. 97." > ~~~~~ > > > It became apparent that something went wrong during Step 4 when the slides were in the glass container (not a coplin jar - we have ten slides that we need to stain so we're using a rectangular glass container that holds ten slides on their sides - it does require a metal handle to move, but the handle is flexible and easy to remove after the glass slide rack has been transferred between containers) of silver solution in the water bath because there was lots of precipitate on the slides and floating on the surface of the silver solution. > > In my first test, I used five test slides (extra slides that we cut from the same blocks that were submitted to us). I deparaffinized them in coplin jars (moving them with plastic forceps) and hydrated them to deionized water. I transferred the slides to a glass slide rack that holds ten slides on their sides, added five blank slides that were rinsed in deionized water (so that the displacement of reagents would be equivalent to when we stain our ten "real" slides after testing is complete), and completed Step 2. I don't recall exactly how long the glass container of silver solution and the glass container of deionized water had been heating up in the water bath, but I would estimate ~15-30 minutes. The thermometer said that the water in the bath (not inside the containers) reached ~60-65 degrees Celsius. The silver solution was clear and colorless when I made it up, but by the time I put the slides into the warm silver solution, the solution was beginning to turn a light brown color (though it was still clear and I did not see any precipitate floating around). I removed the metal handle of the glass slide rack after the rack was transferred into the silver solution, but the metal handle did dip into the silver solution briefly. At some point, I noticed precipitate floating around of the surface of the silver solution. After ~80 minutes, I used plastic forceps to remove one test slide from the warm silver solution, dipped it several times into the warm deionized water to rinse it, and wiped off the back of the slide with gauze. The amount of precipitate was so extreme that the gauze did nearly nothing. I showed the slide to one of our pathologists and they could hardly see beyond the precipitate, but said that they couldn't see any staining of the structure that they were looking for (I forget exactly what it was, but I know it's supposed to turn black). > > In my second test (to see if the metal holder was the problem) that I performed immediately after the first test, I used one test slide. I deparaffinized it in the same coplin jars as before (moving it with plastic forceps) and hydrated it to deionized water. I used new glass containers for the periodic acid and deionized water rinse in Step 2, for making the silver solution in Step 3, and for the warm silver solution and warm deionized water in Step 4. I used plastic forceps to move the slide into the periodic acid, and propped it up in the container so that no glass rack or metal handle was used at all. I used plastic forceps to transfer the slide to the deionized water rinse, and dunked it several times and swished the slide around a bit. I used plastic forceps to transfer the slide into the warm(-ish) silver solution and propped it up against the side again. After approximately 20 minutes, I saw precipitate floating around, and I used plastic forceps to remove the slide from the silver solution. I dipped the slide into the warm(-ish) deionized water several times, and saw that the precipitate was again covering the slide and the tissue so I stopped there for the day. > > We purchased all of the reagents listed in the above procedure from Rowley Biochemical (except for the Glacial Acetic Acid mentioned in Step 7, but I didn't even get that far). > > Questions: > > 1. Could this indicate that the acid-washing was not done correctly? I made up a ~1% Hydrochloric Acid solution (with deionized water) and filled a plastic bin with the solution (I rinsed the bin with deionized water first). I then submerged all glassware (in several batches) for at least five minutes, then rinsed well with deionized water (not by filling a bin - I just used the hose of deionized water in our lab sink and poured it over the glassware) and left them to air-dry overnight. > > 2. Are using acid-washed glassware and avoiding metal even necessary precautions after the sodium thiosulphate in Step 6? I read that sodium thiosulphate "stops the reaction," and the procedure stops specifically saying to use deionized water after Step 6 and starts saying to use just "water" or "tap water." My lab refers to our waters as either "tap" or "deionized," so I'm assuming that using my deionized water is fine when the procedure calls for "distilled" or "dechlorinated." > > I don't even know enough to ask more questions, but I'm sure many more will arise after I test the stain again next week, so I welcome any and all advice about silver stains, acid-cleaning glassware, and literally anything else... > > Thank you!!! > > Jordan H. > University of Michigan > Ann Arbor, MI > ********************************************************** > Electronic Mail is not secure, may not be read every day, and should > not be used for urgent or sensitive issues > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li > stinfo/histonet__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrV > mY_FPmCDYnGHGszj4cR01z2-BarQ$ > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrVmY_FPmCDYnGHGszj4cR01z2-BarQ$ This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ------------------------------ Message: 7 Date: Thu, 23 Sep 2021 18:28:03 -0500 From: Colleen Forster To: "Tony Henwood (SCHN)" Cc: Bryan Llewellyn , Jordan , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions Message-ID: Content-Type: text/plain; charset="UTF-8" Make sure the periodic acid is made fresh EACH time you run the stain. That can also make a big difference in the stain quality. Colleen Forster HT(ASCP)QIHC On Thu, Sep 23, 2021 at 6:14 PM Tony Henwood (SCHN) via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I agree with Bryan, > > The introduction of thiosemicarbazide before the silver step improves > the staining immensely. > > I would also look at the periodic acid. Is it too dilute, though 0.5% > should work? I usually cover this by using a 1% solution for 20 minutes. > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children?s Hospital at Westmead Adjunct > Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, > Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: Bryan Llewellyn via Histonet [mailto: > histonet at lists.utsouthwestern.edu] > Sent: Friday, 24 September 2021 7:47 AM > To: Jordan ; Histonet < > histonet at lists.utsouthwestern.edu> > Subject: Re: [Histonet] Jones' Methenamine Silver Stain for Basement > Membranes of Kidney - Issues and Questions > > Hi, > Try the method given in StainsFile at: > https://urldefense.com/v3/__http://stainsfile.info/stain/metallic/jone > s.htm__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrVmY_FPmCDYn > GHGszj4cR01CJSb57Q$ > > Bryan Llewellyn > > > Hood, Jordan via Histonet wrote: > > Hello, > > > > I'm new to histology (and new to histonet), and I work in a small > histology lab specializing in animal tissues that receives > requests/submissions from researchers. I tried (and failed) to perform > a Jones' Methenamine Silver stain on a client's submission of pig > kidneys (formalin-fixed, paraffin-embedded, cut at 2.5 microns), and I > need some help troubleshooting this stain since my co-workers are > stumped, too. I used the following procedure from Rowley Biochemical: > > > > > > ~~~~~ > > "Fixation: 10% Buffered Neutral Formalin (F-113) or Bouin's Solution > > (F-40) or Zenker's (F-155) > > > > Sections: Paraffin, 2 microns > > > > Procedure: Acid washed glassware must be used!!!! > > 1. Deparaffinize and hydrate to distilled water. > > 2. Oxidize in Periodic Acid 0.5% (F-396-1) 11 minutes. Wash in > chloride-free water. > > 3. Prepare Methenamine Silver solution by mixing: 42.5 ml > > Methenamine 3% > (F-396-2), 2.5 ml Silver Nitrate, 5% (F-396-3) and 12.0 ml Borate > Buffer, pH 8.2 (F-396-4). > > 4. Place slides in the solution and the entire jar in a water bath > > at > 70?C for approx. 60-75 minutes. Check under microscope when slides > appear medium brown microscopically. Every 10 minutes, once the medium > brown color has been established, rinse a slide in 70?C, chloride free > water and check under a microscope. Rinse again in hot water and > return to the hot staining solution. As the staining time approaches > the end point, check the slides, as above, every 1-2 minutes. The > entire procedure must be performed quickly to prevent an uneven > staining of the tissues. The slides should exhibit a > brownish- yellow background, intense black reticulum fibers, and black > basement membranes. If the slides become oversaturated, i.e. too > black, destain in a dilute Potassium Ferricyanide Solution (F-396-11) > for one or two dips. > > 5. Rinse well in distilled water. Tone in Gold Chloride 0.2% > > (F-396-5), > 1 minute. If sections are overtoned place in Sodium Metabisulfite, 3% > (F-396-12) for 1-3 minutes. Rinse well in distilled water. > > 6. Sodium Thiosulfate 3% (F-396-9), 1-2 miutes. Wash in running tap > water, 10 minutes. Rinse well in distilled water. > > 7. Stain in Harris' Hematoxylin (F-396-6) containing 2-4ml of > > Glacial > Acetic Acid per 100 ml for 5-15 minutes. Wash in water. > > 8. Differentiate in Acid Alcohol 1% (F-396-13) until the sections > > turn > red. > > 9. Blue section in Ammonia Water, 0.3% (F-396-14). Wash thoroughly. > > 10. Counterstain in Eosin Y, 1%, Alcoholic Solution (F-396-7). > > 11. Dehydrate in 95% alcohol, absolute alcohol and clear in xylene 3 > changes each. Mount. > > > > Stain Results: > > Basement membranes, reticulum fibers: Black > > Nuclei: Blue > > Cytoplasm, collagen, connective tissue: Pink-orange > > > > References: Jones, D.B., Amer.J.Path. 27:99 (1951). AFIP Manual of > Histolocical Staining Methods, 3rd ed., Ed. L. Luna: NY: McGraw-Hill > Publications, c. 1968, p. 97." > > ~~~~~ > > > > > > It became apparent that something went wrong during Step 4 when the > slides were in the glass container (not a coplin jar - we have ten > slides that we need to stain so we're using a rectangular glass > container that holds ten slides on their sides - it does require a > metal handle to move, but the handle is flexible and easy to remove > after the glass slide rack has been transferred between containers) of > silver solution in the water bath because there was lots of > precipitate on the slides and floating on the surface of the silver solution. > > > > In my first test, I used five test slides (extra slides that we cut > > from > the same blocks that were submitted to us). I deparaffinized them in > coplin jars (moving them with plastic forceps) and hydrated them to > deionized water. I transferred the slides to a glass slide rack that > holds ten slides on their sides, added five blank slides that were > rinsed in deionized water (so that the displacement of reagents would > be equivalent to when we stain our ten "real" slides after testing is > complete), and completed Step 2. I don't recall exactly how long the > glass container of silver solution and the glass container of > deionized water had been heating up in the water bath, but I would > estimate ~15-30 minutes. The thermometer said that the water in the > bath (not inside the containers) reached ~60-65 degrees Celsius. The > silver solution was clear and colorless when I made it up, but by the > time I put the slides into the warm silver solution, the solution was > beginning to turn a light brown color (though it was still clear and I > did not see any precipitate floating around). I removed the metal > handle of the glass slide rack after the rack was transferred into the > silver solution, but the metal handle did dip into the silver solution > briefly. At some point, I noticed precipitate floating around of the > surface of the silver solution. After ~80 minutes, I used plastic > forceps to remove one test slide from the warm silver solution, dipped > it several times into the warm deionized water to rinse it, and wiped > off the back of the slide with gauze. The amount of precipitate was so > extreme that the gauze did nearly nothing. I showed the slide to one > of our pathologists and they could hardly see beyond the precipitate, > but said that they couldn't see any staining of the structure that they were looking for (I forget exactly what it was, but I know it's supposed to turn black). > > > > In my second test (to see if the metal holder was the problem) that > > I > performed immediately after the first test, I used one test slide. I > deparaffinized it in the same coplin jars as before (moving it with > plastic > forceps) and hydrated it to deionized water. I used new glass > containers for the periodic acid and deionized water rinse in Step 2, > for making the silver solution in Step 3, and for the warm silver > solution and warm deionized water in Step 4. I used plastic forceps to > move the slide into the periodic acid, and propped it up in the > container so that no glass rack or metal handle was used at all. I > used plastic forceps to transfer the slide to the deionized water > rinse, and dunked it several times and swished the slide around a bit. > I used plastic forceps to transfer the slide into the warm(-ish) silver solution and propped it up against the side again. > After approximately 20 minutes, I saw precipitate floating around, and > I used plastic forceps to remove the slide from the silver solution. I > dipped the slide into the warm(-ish) deionized water several times, > and saw that the precipitate was again covering the slide and the > tissue so I stopped there for the day. > > > > We purchased all of the reagents listed in the above procedure from > Rowley Biochemical (except for the Glacial Acetic Acid mentioned in > Step 7, but I didn't even get that far). > > > > Questions: > > > > 1. Could this indicate that the acid-washing was not done correctly? > > I > made up a ~1% Hydrochloric Acid solution (with deionized water) and > filled a plastic bin with the solution (I rinsed the bin with > deionized water first). I then submerged all glassware (in several > batches) for at least five minutes, then rinsed well with deionized > water (not by filling a bin - I just used the hose of deionized water > in our lab sink and poured it over the glassware) and left them to air-dry overnight. > > > > 2. Are using acid-washed glassware and avoiding metal even necessary > precautions after the sodium thiosulphate in Step 6? I read that > sodium thiosulphate "stops the reaction," and the procedure stops > specifically saying to use deionized water after Step 6 and starts > saying to use just "water" or "tap water." My lab refers to our waters > as either "tap" or "deionized," so I'm assuming that using my > deionized water is fine when the procedure calls for "distilled" or "dechlorinated." > > > > I don't even know enough to ask more questions, but I'm sure many > > more > will arise after I test the stain again next week, so I welcome any > and all advice about silver stains, acid-cleaning glassware, and > literally anything else... > > > > Thank you!!! > > > > Jordan H. > > University of Michigan > > Ann Arbor, MI > > ********************************************************** > > Electronic Mail is not secure, may not be read every day, and should > > not be used for urgent or sensitive issues > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/ > > listinfo/histonet__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6 > > HlrVmY_FPmCDYnGHGszj4cR01z2-BarQ$ > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li > stinfo/histonet__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrV > mY_FPmCDYnGHGszj4cR01z2-BarQ$ > > This message is intended for the addressee named and may contain > confidential information. If you are not the intended recipient, > please delete it and notify the sender. > > Views expressed in this message are those of the individual sender, > and are not necessarily the views of NSW Health or any of its entities. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li > stinfo/histonet__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrV > mY_FPmCDYnGHGszj4cR01z2-BarQ$ > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrVmY_FPmCDYnGHGszj4cR01z2-BarQ$ ------------------------------ End of Histonet Digest, Vol 214, Issue 14 ***************************************** From relia1 at earthlink.net Tue Sep 28 10:54:45 2021 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 28 Sep 2021 11:54:45 -0400 Subject: [Histonet] Tired of Cutting and Embedding ALL Day? Want to learn MORE? Want to DO More? Message-ID: <000001d7b481$29ccafa0$7d660ee0$@earthlink.net> Hi Histopeeps! Tired of Cutting and Embedding ALL DAY? Do you want to DO more? LEARN More? My Clients want that TOO!! I have opportunities nationwide with top labs offering YOU the chance to take it to the next level. My hottest opportunities are in: Florida California New York Illinois Colorado North Carolina Contact Me: Pam Barker relia1 at earthlink.net or call/txt 407-353-5070! #ilovemyhistopeeps #jobs4myhistopeeps Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From mcaliger at alliedsearchpartners.com Tue Sep 28 11:15:26 2021 From: mcaliger at alliedsearchpartners.com (Melissa Caliger) Date: Tue, 28 Sep 2021 16:15:26 +0000 Subject: [Histonet] Job Alert - Tarpon Springs, Florida Message-ID: Histotech-Tarpon Springs, FL (34689) Benefits * Health Insurance * Dental Insurance and Vision Insurance * Basic Life & Disability * Retirement * Miscellaneous benefits * Relocation Assistance Job Description Histotech (Healthcare Sciences) Job Responsibilities Position Title: Histotech Location: Tarpon Springs, FL Shift: Day Shift 5am-1:30pm As a Histotechnologist, you will perform daily, routine, and specialized histology techniques and procedures, including: embedding, microtomy, grossing, processing, h&e staining, special staining, immunohistochemistry (IHC), and equipment maintenance. Job Requirements Successful candidates for the Histotechnologist will have certification by American Society for Clinical Pathology (ASCP) as a Histotechnician (HT) or Histotechnologist (HTL) preferred. Additional requirements of the histology technologist role include: * Associates or Bachelor's degree in Histotechnology or related Life Sciences/Health Sciences degree. * 1-3 Years of experience working as a Histotechnologist or Histotechnician * Experience in embedding tissue, microtomy, H&E staining, labeling slides, filing slides, accessioning and grossing tissue * Must be a team player and be able to work in a fast paced/busy environment * Must be well-versed in computers and a quick learner * Florida Clinical Histology Technician/Technologist license Want to discuss a position? Please send resume & schedule a meeting: https://calendly.com/mcaliger Melissa Caliger Allied Search Partners AN MRINETWORK MEMBER http://www.alliedsearchpartners.com From krapp at rchsd.org Tue Sep 28 12:29:08 2021 From: krapp at rchsd.org (Rapp, Keith) Date: Tue, 28 Sep 2021 17:29:08 +0000 Subject: [Histonet] H Pylori IHC Message-ID: Hello, we have just purchased the Leica Bond Max IHC stainer and love it. It's easy to use and produces beautiful stains. However, Helico Pylori has been giving us problems with weak and incomplete staining (not all of the organisms staining). Is there anyone using the Bond Max and successfully staining H. Pylori? We have tried two different antibody vendors, Leica and Biocare Medical giving similar results. We have also tried several different Antigen Retrieval methods. Thank you, Keith Rapp Histology Technical Specialist Rady Children's Hospital Department of Pathology krapp at rchsd.org 858-966-1700 x225181 From historuiz at yahoo.com Tue Sep 28 13:18:08 2021 From: historuiz at yahoo.com (Misty) Date: Tue, 28 Sep 2021 13:18:08 -0500 Subject: [Histonet] Histotech Ocean Springs, MS References: <6C9F4BB2-216A-4980-ABCB-79B3132CDCE6.ref@yahoo.com> Message-ID: <6C9F4BB2-216A-4980-ABCB-79B3132CDCE6@yahoo.com> ? ? We have open positions for histotechs at Singing River Health System Ocean Springs, MS. Thank you Misty Ruiz https://singingriverhealthsystem.csod.com/ux/ats/careersite/1/home/requisition/6385?c=singingriverhealthsystem Sent from my iPhone From kpfefferle at carisls.com Tue Sep 28 20:07:21 2021 From: kpfefferle at carisls.com (Pfefferle, Katrina) Date: Wed, 29 Sep 2021 01:07:21 +0000 Subject: [Histonet] Glass Slide QC and Control Tissue Procurement Message-ID: Hello fellow histotechnologists! I was wondering if any of you might have some advice or information on the following two subjects: * Glass slide QC (plus (+) slides used for IHC) - do any of you have a QC process for these that may differ from just running IHC on a small sample group from a shipment of slides, such as performing any kind of electron microscopy or other analyses to determine if the coating on the slides is consistent and adequate? * Control Tissue Procurement-I know many of you may be in your own surgical pathology labs where you have access to extra surgical tissue that you processed in house to use for constructing IHC controls. For those of you that do not-do you purchase any control tissue elsewhere? Do any of your labs work with other labs in a type of supplier/client capacity to procure viable tissue controls that include well known and documented pre-analytics? Any info you may have is greatly appreciated! Thank you, Katrina Pfefferle, MB, HTL (ASCP) QIHC and ASQ Certified Quality Engineer Supervisor-Quality Control Caris Life Sciences Email: kpfefferle at carisls.com From patpxs at gmail.com Wed Sep 29 08:49:47 2021 From: patpxs at gmail.com (P Sicurello) Date: Wed, 29 Sep 2021 06:49:47 -0700 Subject: [Histonet] Sakura microtomes Message-ID: Hello everyone, Has anyone used, or is familiar with, the Sakura SRM 300 LT microtome? Sincerely, Paula Sicurello Sent from my iPhone From katherine at ka-recruiting.com Thu Sep 30 12:57:39 2021 From: katherine at ka-recruiting.com (Katherine Marano) Date: Thu, 30 Sep 2021 13:57:39 -0400 Subject: [Histonet] New Histotech Opening Message-ID: Hi Histonetters, Hope you are having a great week. I wanted to reach out because I just got a new job opening with a client of mine in the Lansing, Michigan area. They are looking to hire a permanent Histology Technician due to expansive growth. Duties include preparing, grossing, cutting, and staining specimens. ASCP required within 1 year of hire. They are offering competitive compensation and benefits including health, dental, vision, 401K with employer contribution. If you are interested, could you send me a resume and a good time to give you a call? If not, feel free to forward this along! Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From bcooper at chla.usc.edu Thu Sep 30 18:37:04 2021 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Thu, 30 Sep 2021 23:37:04 +0000 Subject: [Histonet] Stat6 control material Message-ID: <009385cf-ef63-4e78-ab5e-61f1fc5558de@chla.usc.edu> Good afternoon Histonet, Do any of you have any STAT6 control material available to share? Please message me offline if you're able to help; we have lots of control materials that we can trade. Thanks, Brian Cooper Histology Supervisor, Children's Hospital Los Angeles Bcooper at chla.usc.edu Sent from my mobile phone CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From rmgriffey at ucdavis.edu Thu Sep 30 19:29:53 2021 From: rmgriffey at ucdavis.edu (Rebecca M Griffey) Date: Fri, 1 Oct 2021 00:29:53 +0000 Subject: [Histonet] Histology Lab Supervisor Position UC Davis Message-ID: HISTOLOGY LABORATORY SUPERVISOR POSITION AT THE UNIVERSITY OF CALIFORNIA, DAVIS We have an opening for a Histology Laboratory Supervisor position in the School of Veterinary Medicine Anatomic Pathology Service. The position requires a BS degree in biological science, certification as HTL or HT, and experience to provide effective supervision and leadership in a histology lab. Experience preferred but not required includes supervisory, IHC, FISH, or digital pathology. Contact rmgriffey at ucdavis.edu Job Posting Here Thank you Becky Rebecca M. Griffey Anatomic Pathology Service Manager William R. Pritchard Veterinary Medical Teaching Hospital 1345 VM3A; 1 Shields Ave. University of California, Davis Davis, CA 95616 Voice: 530-752-1369 Fax: 530-754-1400 rmgriffey at ucdavis.edu #1 Ranked Veterinary School in the Nation Residency Training in Anatomic Pathology of UC Davis VMTH Anatomic Pathology Service