[Histonet] Dry Film Coverslipping?

[Rene J Buesa]

 A Sakura film cover slipper will not work without xylene.Now that you have decided to go "xylene free" just go "all the way in" and after staining the slides DRY them in an oven at 60ºC during 5 minutes and cover.If you want further information on "cover slipping without xylene" send me your e-mail address and I will send you a copy of a short paper I published on this subject.René 
    On Wednesday, October 6, 2021, 01:17:48 PM GMT-4, Cates, Julia via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
 
 Hello Histonet,

I have been asked to consider going Xylene free.  The only hang up that I am having is the coverslipping part.  We have an old Sakura Film coverslipper and I don't know how that would work without xylene.  When I did some searching in the archives, I found some comments made by Rene, that he used this type of coverslipper on oven dried slides but never referenced how.  Does anyone use this method in your lab?  If so, would you share your protocol and are there any problems to consider?

Thanks,

Julia C.


This message (including any attachments) is intended only for the use of the individual or entity to which it is addressed and may contain information that is non-public, proprietary, privileged, confidential, and exempt from disclosure under applicable law or may constitute as attorney work product. If you are not the intended recipient, you are hereby notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, notify us immediately by telephone and (i) destroy this message if a facsimile or (ii) delete this message immediately if this is an electronic communication. Thank you.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet