From Nancy.Schmitt at mercyhealth.com Mon Oct 4 10:16:31 2021 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Mon, 4 Oct 2021 15:16:31 +0000 Subject: [Histonet] dissect aid Message-ID: Happy Monday! For those using Dissect Aid (Statlab) - are you re-using this product for multiple specimens? Can you please share what your process is for this? Much appreciated, Nancy Schmitt MLT, HT(ASCP) MercyOne Dubuque Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From tbraud at holyredeemer.com Mon Oct 4 12:48:32 2021 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 4 Oct 2021 17:48:32 +0000 Subject: [Histonet] Dissect Aid - Histonet Digest, Vol 215, Issue 2 Message-ID: <48E053DDF6CE074DB6A7414BA05403F8022F4CE90A@HRHEX03-HOS.holyredeemer.local> We do not re-use. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Monday, October 4, 2021 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 215, Issue 2 CAUTION: This email originated from outside Redeemer Health. Do not click links or open attachments unless you recognize the sender and know the content is safe. Contact our IT Support Center at 215-938-3900 with questions. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. dissect aid (Nancy Schmitt) ---------------------------------------------------------------------- Message: 1 Date: Mon, 4 Oct 2021 15:16:31 +0000 From: Nancy Schmitt To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] dissect aid Message-ID: Content-Type: text/plain; charset="us-ascii" Happy Monday! For those using Dissect Aid (Statlab) - are you re-using this product for multiple specimens? Can you please share what your process is for this? Much appreciated, Nancy Schmitt MLT, HT(ASCP) MercyOne Dubuque Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 215, Issue 2 **************************************** From ccorbin at bloomu.edu Mon Oct 4 15:58:17 2021 From: ccorbin at bloomu.edu (Corbin, Clay) Date: Mon, 4 Oct 2021 20:58:17 +0000 Subject: [Histonet] Fixative in diff-quick Message-ID: Hey folks, I am shopping for a diff-quick kit. However, all I really need is the fixative. Generally, there is a blue stain (triarylmethane) added to the methanol in the fixative solution. I have a giant jug of lab grade methanol. What would I lose by using methanol alone compared to the fixative solution included in a diff-quick kit? Thanks! Clay Clay Corbin, PhD Professor of Biology Bloomsburg University From llewllew at shaw.ca Mon Oct 4 16:40:53 2021 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Mon, 4 Oct 2021 14:40:53 -0700 Subject: [Histonet] Fixative in diff-quick In-Reply-To: References: Message-ID: <89ffb821-c41c-4e82-7b99-b9e378cea52b@shaw.ca> Diff Quick appears to be a modified Field's stain. The blue dye in the methanol is one of the modifications. Field's stain gives much the same staining, so using plain methanol should be of no concern. The simplest way to find out is surely to try it and see. http://stainsfile.info/stain/micro/field.htm Bryan Llewellyn Corbin, Clay via Histonet wrote: > Hey folks, > I am shopping for a diff-quick kit. However, all I really need is the fixative. Generally, there is a blue stain (triarylmethane) added to the methanol in the fixative solution. I have a giant jug of lab grade methanol. What would I lose by using methanol alone compared to the fixative solution included in a diff-quick kit? > Thanks! > Clay > > Clay Corbin, PhD > Professor of Biology > Bloomsburg University > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From chak_bou at yahoo.com Tue Oct 5 07:49:47 2021 From: chak_bou at yahoo.com (Chakib Boussahmain) Date: Tue, 5 Oct 2021 12:49:47 +0000 (UTC) Subject: [Histonet] Tissue Quality References: <671944432.1235051.1633438187745.ref@mail.yahoo.com> Message-ID: <671944432.1235051.1633438187745@mail.yahoo.com> Hi Guys, We normally buy human tissue blocks to use them as controlsor to develop IF assays in our laboratory. However, most of these blocksappeared to be fixed or processed adequately. I am wondering if anybody has amethod to assess the quality of these tissue blocks besides H&E stain. Forinstance, is there an IHC marker or a panel of the markers that I can use as agood indicator to qualify these tissue blocks? Thank you so much in advance for any suggestions! Best Chakib From sbonner at pathregional.com Tue Oct 5 11:19:29 2021 From: sbonner at pathregional.com (Silvia Bonner) Date: Tue, 5 Oct 2021 16:19:29 +0000 Subject: [Histonet] microtome Message-ID: Hello, We are in the market for a new/refurbished microtome. We currently have a Micron HM355s, and Micron HM 335E. We are interested in buying something comparable. Any suggestions? We are looking at Micron and Leica. Good or bad reviews would be appreciated. Thanks, Silvia Bonner, BS, HT(ASCP) CM Histology Supervisor sbonner at pathregional.com Pathologists? Regional Laboratory Anatomical Pathology 2841 Juniper Dr. Suite 1 Lewiston, ID 83501 (208)746-5533 This email may contain physician, patient and/or attorney material that is confidential or privileged for the sole use of the intended recipient. YOU ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED. If you are not the intended recipient, please notify us immediately by telephone at (208) 746-0516 or (208) 746-5533 and delete all copies. This email may contain physician, patient and/or attorney material that is confidential or privileged for the sole use of the intended recipient. YOU ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED. If you are not the intended recipient, please notify us immediately by telephone at (208) 746-0516 or (208) 746-5533 and delete all copies. Pathologist's Regional Laboratory From Valerie.Hannen at parrishmed.com Tue Oct 5 11:30:46 2021 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Tue, 5 Oct 2021 16:30:46 +0000 Subject: [Histonet] FW: Biopsy processing In-Reply-To: <46ff805667fd4c069705fab75b175398@PMSVRLEX01.parrishmed.local> References: <46ff805667fd4c069705fab75b175398@PMSVRLEX01.parrishmed.local> Message-ID: From: Hannen, Valerie Sent: Monday, October 04, 2021 2:54 PM To: 'histonet at list.utsouthwestern.edu' Subject: FW: Biopsy processing From: Hannen, Valerie Sent: Monday, October 4, 2021 11:03 AM To: 'histonet at list.utsouthwestern.edu' Subject: Biopsy processing Good Morning All... My Pathologist is wondering if there is any benefit to having a separate processing run just for small biopsies? He said that what is seeing now, he has no issue with, the sections are just as good as all the other tissue sections. He just asked for me to get the consensus of the group. Thank You in advance!! Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Avenue Titusville, Florida 32796 P: 321-268-6333 Ext. 7506 F: 321-268-6149 valerie.hannen at parrishmed.com www.parrishmed.com From wilfong1923 at gmail.com Tue Oct 5 12:35:37 2021 From: wilfong1923 at gmail.com (John Frazier) Date: Tue, 5 Oct 2021 13:35:37 -0400 Subject: [Histonet] Histonet Digest, Vol 215, Issue 3 In-Reply-To: References: Message-ID: If you are looking to prioritize certain tissues slides to the pathologist, I think separate small biopsy runs are a great idea. You can process them with a much shorter run cycle.(90-120 min). You can actually do several runs per day. This is a good way of decreasing your TAT for all blocks and putting the signout TAT back in the pathologist's hands. If you are interested, I can suggest a run schedule. *John Frazier* *MT(ASCP), MBA, * *Lean 6 Six Sigma Black Belt* *Healthcare Consultant* *C - 302-310-7567 * *H - 704-847-0566* *wilfong1923 at gmail.com * On Tue, Oct 5, 2021 at 1:00 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Re: Dissect Aid - Histonet Digest, Vol 215, Issue 2 (Terri Braud) > 2. Fixative in diff-quick (Corbin, Clay) > 3. Re: Fixative in diff-quick (Bryan Llewellyn) > 4. Tissue Quality (Chakib Boussahmain) > 5. microtome (Silvia Bonner) > 6. FW: Biopsy processing (Hannen, Valerie) > > > > ---------- Forwarded message ---------- > From: Terri Braud > To: "histonet at lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Mon, 4 Oct 2021 17:48:32 +0000 > Subject: Re: [Histonet] Dissect Aid - Histonet Digest, Vol 215, Issue 2 > We do not re-use. > > Terri L. Braud, HT(ASCP) > HNL Laboratories for > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3689 > Fax: 215-938-3874 > Honesty > AccouNtability > AgiLity > CoLlaboration > CoMpassion > > -----Original Message----- > From: histonet-request at lists.utsouthwestern.edu < > histonet-request at lists.utsouthwestern.edu> > Sent: Monday, October 4, 2021 1:00 PM > To: histonet at lists.utsouthwestern.edu > Subject: [EXTERNAL] Histonet Digest, Vol 215, Issue 2 > > CAUTION: This email originated from outside Redeemer Health. Do not click > links or open attachments unless you recognize the sender and know the > content is safe. Contact our IT Support Center at 215-938-3900 with > questions. > > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific than > "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. dissect aid (Nancy Schmitt) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 4 Oct 2021 15:16:31 +0000 > From: Nancy Schmitt > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] dissect aid > Message-ID: > < > CH2PR10MB40698BE87B2D69782E45D007E0AE9 at CH2PR10MB4069.namprd10.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > Happy Monday! > For those using Dissect Aid (Statlab) - are you re-using this product for > multiple specimens? > Can you please share what your process is for this? > Much appreciated, > Nancy Schmitt MLT, HT(ASCP) > MercyOne Dubuque > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 215, Issue 2 > **************************************** > > > > > > > ---------- Forwarded message ---------- > From: "Corbin, Clay" > To: "histonet at lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Mon, 4 Oct 2021 20:58:17 +0000 > Subject: [Histonet] Fixative in diff-quick > Hey folks, > I am shopping for a diff-quick kit. However, all I really need is the > fixative. Generally, there is a blue stain (triarylmethane) added to the > methanol in the fixative solution. I have a giant jug of lab grade > methanol. What would I lose by using methanol alone compared to the > fixative solution included in a diff-quick kit? > Thanks! > Clay > > Clay Corbin, PhD > Professor of Biology > Bloomsburg University > > > > ---------- Forwarded message ---------- > From: Bryan Llewellyn > To: Clay , Histonet > > Cc: > Bcc: > Date: Mon, 4 Oct 2021 14:40:53 -0700 > Subject: Re: [Histonet] Fixative in diff-quick > Diff Quick appears to be a modified Field's stain. The blue dye in the > methanol is one of the modifications. Field's stain gives much the same > staining, so using plain methanol should be of no concern. The simplest > way to find out is surely to try it and see. > > http://stainsfile.info/stain/micro/field.htm > > Bryan Llewellyn > > > Corbin, Clay via Histonet wrote: > > Hey folks, > > I am shopping for a diff-quick kit. However, all I really need is the > fixative. Generally, there is a blue stain (triarylmethane) added to the > methanol in the fixative solution. I have a giant jug of lab grade > methanol. What would I lose by using methanol alone compared to the > fixative solution included in a diff-quick kit? > > Thanks! > > Clay > > > > Clay Corbin, PhD > > Professor of Biology > > Bloomsburg University > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ---------- Forwarded message ---------- > From: Chakib Boussahmain > To: "histonet at lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Tue, 5 Oct 2021 12:49:47 +0000 (UTC) > Subject: [Histonet] Tissue Quality > > Hi Guys, > > We normally buy human tissue blocks to use them as controlsor to develop > IF assays in our laboratory. However, most of these blocksappeared to be > fixed or processed adequately. I am wondering if anybody has amethod to > assess the quality of these tissue blocks besides H&E stain. Forinstance, > is there an IHC marker or a panel of the markers that I can use as agood > indicator to qualify these tissue blocks? > > Thank you so much in advance for any suggestions! > > Best > > Chakib > > > > > > ---------- Forwarded message ---------- > From: Silvia Bonner > To: "histonet at lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Tue, 5 Oct 2021 16:19:29 +0000 > Subject: [Histonet] microtome > Hello, > > We are in the market for a new/refurbished microtome. We currently have a > Micron HM355s, and Micron HM 335E. We are interested in buying something > comparable. Any suggestions? We are looking at Micron and Leica. Good or > bad reviews would be appreciated. > > Thanks, > > > Silvia Bonner, BS, HT(ASCP) CM > > Histology Supervisor > > > > sbonner at pathregional.com > > Pathologists? Regional Laboratory > > Anatomical Pathology > > 2841 Juniper Dr. Suite 1 > > Lewiston, ID 83501 > > (208)746-5533 > > > > This email may contain physician, patient and/or attorney material that > is confidential or privileged for the sole use of the intended recipient. > YOU ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR > COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY > PROHIBITED. If you are not the intended recipient, please notify us > immediately by telephone at (208) 746-0516 or (208) 746-5533 and delete all > copies. > > > > This email may contain physician, patient and/or attorney material that is > confidential or privileged for the sole use of the intended recipient. YOU > ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR > COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY > PROHIBITED. If you are not the intended recipient, please notify us > immediately by telephone at (208) 746-0516 or (208) 746-5533 and delete all > copies. > > Pathologist's Regional Laboratory > > > > > > ---------- Forwarded message ---------- > From: "Hannen, Valerie" > To: "Histonet at lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Tue, 5 Oct 2021 16:30:46 +0000 > Subject: [Histonet] FW: Biopsy processing > > > From: Hannen, Valerie > Sent: Monday, October 04, 2021 2:54 PM > To: 'histonet at list.utsouthwestern.edu' > Subject: FW: Biopsy processing > > > > From: Hannen, Valerie > Sent: Monday, October 4, 2021 11:03 AM > To: 'histonet at list.utsouthwestern.edu' > Subject: Biopsy processing > > Good Morning All... My Pathologist is wondering if there is any > benefit to having a separate processing run just for small biopsies? He > said that what is seeing now, he has no issue with, the sections are just > as good as all the other tissue sections. He just asked for me to get the > consensus of the group. > > Thank You in advance!! > > > > > Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Avenue > Titusville, Florida 32796 > P: 321-268-6333 Ext. 7506 > F: 321-268-6149 > valerie.hannen at parrishmed.com > www.parrishmed.com > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cxk340 at case.edu Tue Oct 5 13:17:40 2021 From: cxk340 at case.edu (Chaitanya Kolluru) Date: Tue, 5 Oct 2021 14:17:40 -0400 Subject: [Histonet] Using LR white with a tungsten carbide knife Message-ID: Hi, I?m looking for some advice on using LR white on an osmium stained tissue. Unfortunately, I don?t have access to an ultra-microtome or diamond knives. Has someone here tried sectioning LR white with tungsten carbide knives on a motorized rotary microtome? Are there any sectioning artifacts that I might see? Also, LR white seems to be available in three grades - soft, medium and hard. Is the soft grade recommended with my setup? Thank you, Chaitanya From john.garratt at ciqc.ca Tue Oct 5 13:23:05 2021 From: john.garratt at ciqc.ca (John Garratt) Date: Tue, 05 Oct 2021 18:23:05 +0000 Subject: [Histonet] FW: Biopsy processing In-Reply-To: References: <46ff805667fd4c069705fab75b175398@PMSVRLEX01.parrishmed.local> Message-ID: We (CPQA) ran our first EQA to included a review of tissue processing and staining of various tissue and the report might be of interest to you and others. Contact me directly if you, or anybody else, would like a copy of the report. John Sent from ProtonMail for iOS On Tue, Oct 5, 2021 at 9:30 AM, Hannen, Valerie via Histonet wrote: > From: Hannen, Valerie > Sent: Monday, October 04, 2021 2:54 PM > To: 'histonet at list.utsouthwestern.edu' > Subject: FW: Biopsy processing > > From: Hannen, Valerie > Sent: Monday, October 4, 2021 11:03 AM > To: 'histonet at list.utsouthwestern.edu' > Subject: Biopsy processing > > Good Morning All... My Pathologist is wondering if there is any benefit to having a separate processing run just for small biopsies? He said that what is seeing now, he has no issue with, the sections are just as good as all the other tissue sections. He just asked for me to get the consensus of the group. > > Thank You in advance!! > > Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Avenue > Titusville, Florida 32796 > P: 321-268-6333 Ext. 7506 > F: 321-268-6149 > valerie.hannen at parrishmed.com > www.parrishmed.com > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Tue Oct 5 14:17:01 2021 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue, 5 Oct 2021 19:17:01 +0000 Subject: [Histonet] Fw: Fixative in diff-quick In-Reply-To: References: , Message-ID: <1633461421894.99531@health.nsw.gov.au> Hi Clay, The first solution is methanol. So lab grade methanol will do. The blue colour is from an innocuous dye added to differentiate from water (and ethanol used in cytology fixation). This helps us poor cytologists when we are doing ROSE at FNAs. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Corbin, Clay via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, 5 October 2021 7:58 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fixative in diff-quick Hey folks, I am shopping for a diff-quick kit. However, all I really need is the fixative. Generally, there is a blue stain (triarylmethane) added to the methanol in the fixative solution. I have a giant jug of lab grade methanol. What would I lose by using methanol alone compared to the fixative solution included in a diff-quick kit? Thanks! Clay Clay Corbin, PhD Professor of Biology Bloomsburg University _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From michelle.perrins at uct.ac.za Wed Oct 6 07:24:55 2021 From: michelle.perrins at uct.ac.za (Michelle Perrins) Date: Wed, 6 Oct 2021 12:24:55 +0000 Subject: [Histonet] Vacuum packing of formalin fixed tissues for archiving Message-ID: Hello I would like to know if any histology laboratory stores their fixed specimens this way and what instrument do you use? Regards Michelle Sent from Mail for Windows Disclaimer - University of Cape Town This email is subject to UCT policies and email disclaimer published on our website at http://www.uct.ac.za/main/email-disclaimer or obtainable from +27 21 650 9111. If this email is not related to the business of UCT, it is sent by the sender in an individual capacity. Please report security incidents or abuse via https://csirt.uct.ac.za/page/report-an-incident.php. From Kelly.Pairan at nationwidechildrens.org Wed Oct 6 11:53:36 2021 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Wed, 6 Oct 2021 16:53:36 +0000 Subject: [Histonet] GLUT-1 and Desmin Message-ID: Good Afternoon, Our current vendor of GLUT-1 and Desmin has a backorder until next year. Would you mind sharing what your institutions use? We run them on Leica Bonds. Thanks, Kelly From JULIA.CATES at AdventHealth.com Wed Oct 6 12:00:59 2021 From: JULIA.CATES at AdventHealth.com (Cates, Julia) Date: Wed, 6 Oct 2021 17:00:59 +0000 Subject: [Histonet] Dry Film Coverslipping? Message-ID: Hello Histonet, I have been asked to consider going Xylene free. The only hang up that I am having is the coverslipping part. We have an old Sakura Film coverslipper and I don't know how that would work without xylene. When I did some searching in the archives, I found some comments made by Rene, that he used this type of coverslipper on oven dried slides but never referenced how. Does anyone use this method in your lab? If so, would you share your protocol and are there any problems to consider? Thanks, Julia C. This message (including any attachments) is intended only for the use of the individual or entity to which it is addressed and may contain information that is non-public, proprietary, privileged, confidential, and exempt from disclosure under applicable law or may constitute as attorney work product. If you are not the intended recipient, you are hereby notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, notify us immediately by telephone and (i) destroy this message if a facsimile or (ii) delete this message immediately if this is an electronic communication. Thank you. From mcruz84pr at gmail.com Wed Oct 6 13:09:47 2021 From: mcruz84pr at gmail.com (Maria Cruz) Date: Wed, 6 Oct 2021 14:09:47 -0400 Subject: [Histonet] GLUT-1 and Desmin In-Reply-To: References: Message-ID: The lab I work gets both of these antibodies from Biocare, and we rarely, if ever, experience a back order from that vendor. On Wed, Oct 6, 2021 at 1:06 PM Pairan, Kelly via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good Afternoon, > Our current vendor of GLUT-1 and Desmin has a backorder until next year. > Would you mind sharing what your institutions use? We run them on Leica > Bonds. > > Thanks, > Kelly > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JULIA.CATES at AdventHealth.com Wed Oct 6 12:00:59 2021 From: JULIA.CATES at AdventHealth.com (Cates, Julia) Date: Wed, 6 Oct 2021 17:00:59 +0000 Subject: [Histonet] Dry Film Coverslipping? Message-ID: Hello Histonet, I have been asked to consider going Xylene free. The only hang up that I am having is the coverslipping part. We have an old Sakura Film coverslipper and I don't know how that would work without xylene. When I did some searching in the archives, I found some comments made by Rene, that he used this type of coverslipper on oven dried slides but never referenced how. Does anyone use this method in your lab? If so, would you share your protocol and are there any problems to consider? Thanks, Julia C. This message (including any attachments) is intended only for the use of the individual or entity to which it is addressed and may contain information that is non-public, proprietary, privileged, confidential, and exempt from disclosure under applicable law or may constitute as attorney work product. If you are not the intended recipient, you are hereby notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, notify us immediately by telephone and (i) destroy this message if a facsimile or (ii) delete this message immediately if this is an electronic communication. Thank you. From tony.henwood at health.nsw.gov.au Wed Oct 6 17:43:41 2021 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 6 Oct 2021 22:43:41 +0000 Subject: [Histonet] Vacuum packing of formalin fixed tissues for archiving In-Reply-To: References: Message-ID: <7741c551a0e547beb73d9b688ab62444@SVDCMBX-MEX024.nswhealth.net> Hi Michelle, We have used the Milestone Tissue Safe Plus unit with Integrated Data Logger card reader to seal formalin-fixed placentas since 2019. Placentas are drained of excess formalin in a chemical hood, placed into a labelled bag and sealed. We instituted the process to reduce the formalin fume leakage that often accompanies the storage of these specimens and to decrease the storage space required. The process has lived up to its aims (formalin fume containment, no liquid leakage and reduced storage requirements) and we are currently sampling the early specimens (3 years in storage) to analyse any adverse morphology and Immunohistochemical staining that might occur. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Michelle Perrins via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 6 October 2021 11:25 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Vacuum packing of formalin fixed tissues for archiving Hello I would like to know if any histology laboratory stores their fixed specimens this way and what instrument do you use? Regards Michelle Sent from Mail for Windows Disclaimer - University of Cape Town This email is subject to UCT policies and email disclaimer published on our website at http://www.uct.ac.za/main/email-disclaimer or obtainable from +27 21 650 9111. If this email is not related to the business of UCT, it is sent by the sender in an individual capacity. Please report security incidents or abuse via https://csirt.uct.ac.za/page/report-an-incident.php. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From rjbuesa at yahoo.com Thu Oct 7 07:54:19 2021 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 7 Oct 2021 12:54:19 +0000 (UTC) Subject: [Histonet] Dry Film Coverslipping? In-Reply-To: References: Message-ID: <1509241347.617448.1633611259496@mail.yahoo.com> A Sakura film cover slipper will not work without xylene.Now that you have decided to go "xylene free" just go "all the way in" and after staining the slides DRY them in an oven at 60?C during 5 minutes and cover.If you want further information on "cover slipping without xylene" send me your e-mail address and I will send you a copy of a short paper I published on this subject.Ren?? On Wednesday, October 6, 2021, 01:17:48 PM GMT-4, Cates, Julia via Histonet wrote: Hello Histonet, I have been asked to consider going Xylene free.? The only hang up that I am having is the coverslipping part.? We have an old Sakura Film coverslipper and I don't know how that would work without xylene.? When I did some searching in the archives, I found some comments made by Rene, that he used this type of coverslipper on oven dried slides but never referenced how.? Does anyone use this method in your lab?? If so, would you share your protocol and are there any problems to consider? Thanks, Julia C. This message (including any attachments) is intended only for the use of the individual or entity to which it is addressed and may contain information that is non-public, proprietary, privileged, confidential, and exempt from disclosure under applicable law or may constitute as attorney work product. If you are not the intended recipient, you are hereby notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, notify us immediately by telephone and (i) destroy this message if a facsimile or (ii) delete this message immediately if this is an electronic communication. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From miranda at alliedsearchpartners.com Thu Oct 7 08:05:42 2021 From: miranda at alliedsearchpartners.com (Miranda Sanchez) Date: Thu, 7 Oct 2021 13:05:42 +0000 Subject: [Histonet] Histotech Position in Southern Oregon Area Message-ID: Hello, Histotech Position in Southern Oregon Area * Monday-Friday (Full Time/Permanent) * 4:30am-12:30pm * Pay Ranges from $25-$35/hr * Benefits * Health, Dental, Vision * Disability * Retirement * Associates or Bachelors Degree Required * 1-3 Years of Experience Required * Relocation Assistance Offered To apply: Please send resume to miranda at alliedsearchpartners.com -Miranda From katy at alliedsearchpartners.com Thu Oct 7 08:06:58 2021 From: katy at alliedsearchpartners.com (Katy Boehm) Date: Thu, 7 Oct 2021 13:06:58 +0000 Subject: [Histonet] Histotech Opportunity in Philly!! Message-ID: Hello, I am looking for a talented Histotechnician to take over a permanent position in the Philadelphia area. Day shift, Mon-Fri, from 9AM-5:30PM. (Certification is preferred) To move forward in the hiring process, or to request the full job description, please email your resume to katy at alliedsearchpartners.com . Thanks for your time & have a wonderful day ? -Katy From jordhood at med.umich.edu Thu Oct 7 11:53:49 2021 From: jordhood at med.umich.edu (Hood, Jordan) Date: Thu, 7 Oct 2021 16:53:49 +0000 Subject: [Histonet] Jones problems In-Reply-To: References: Message-ID: <3cb0bc06b6654d4a980b6984cdc27e62@med.umich.edu> Hi Amos, It definitely helps, thank you! I brought this to my pathologist and she said that it makes complete sense. I plan on ordering some TSC in solid form. Is there a chance that I didn?t do the acid-washing of the glassware correctly, leading to contamination? Or could all of the precipitate floating around on the warm silver solution and on the slides be due to the instability of the silver solution? Thank you so much! Jordan From: Amos Brooks Sent: Saturday, September 25, 2021 8:03 AM To: Hood, Jordan ; histonet at lists.utsouthwestern.edu Subject: Jones problems External Email - Use Caution Hi, When I first did this stain, I had really light staining of the GBM. StainsFile has a note in the procedure that describes thiosemicarbizide. Being a complete nerd, I wanted to see another source as well. Here is a link to an article where it is used... https://pubmed.ncbi.nlm.nih.gov/2482996/ I also make these chemicals freshly each time. I accomplish this by pre-weighing the solids sufficient for a 50ml volume and storing it in an eppendorf tube. When you need it, pop the tube into the requirement volume of water and you're golden. I do this because in addition to having good freshly made solutions, I found the thiosemicarbizide goes bad rather quickly after use. TSC is the lynch pin for the procedure. The silver on it's own does not react very strongly with the aldehydes that are reduced by the periodic acid without the TSC to facilitate the precipitation. I hope this helps, Amos Brooks ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues From beth.villarreal at novartis.com Thu Oct 7 12:29:51 2021 From: beth.villarreal at novartis.com (Villarreal, Beth) Date: Thu, 7 Oct 2021 17:29:51 +0000 Subject: [Histonet] job opportunity for research histologist in Cambridge MA Message-ID: Hello Histonet, We're growing our team! The Cardiovascular and Metabolism Department at the Novartis Institute for Biomedical Research (Cambridge, MA) is hiring a research scientist with strong histology experience. The ideal candidate would have a research background, particularly in mouse models of atherosclerosis. See the link below for a full description and to apply. https://sjobs.brassring.com/TGnewUI/Search/home/HomeWithPreLoad?PageType=JobDetails&partnerid=13617&siteid=5268&Areq=328099BR#jobDetails=2746324_5268 From beth.villarreal at novartis.com Thu Oct 7 12:41:28 2021 From: beth.villarreal at novartis.com (Villarreal, Beth) Date: Thu, 7 Oct 2021 17:41:28 +0000 Subject: [Histonet] job opportunity for research histologist in Cambridge MA Message-ID: Hello Histonet, We're growing our team! The Cardiovascular and Metabolism Department at the Novartis Institute for Biomedical Research (Cambridge, MA) is hiring a research scientist with strong histology experience. The ideal candidate would have a research background, particularly in mouse models of atherosclerosis. Please reach out with any questions. For a full description and to apply: Search for job ID 328099BR at https://www.novartis.com/careers/career-search From TNMayer at mdanderson.org Fri Oct 8 16:34:29 2021 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Fri, 8 Oct 2021 21:34:29 +0000 Subject: [Histonet] Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions Message-ID: Hi Jordan, So wow! You are doing a Jones with an H&E. What you are seeing is common when using a water bath and preheating. While you can perform the stain with this many sections, I would start off with 1-3 slides until you have perfected the technique. I know that makes you have multiple runs, but you don't want to waste the tissue or have a lot of precipitate. 1. I would only preheat silver for 5-10 min to avoid excess precipitation and agitate the solution before immersing your slides. 2. If you use the glass staining rack, make sure no excess water from the water bath gets in. 3. Rinse silver stains 6x with de-I or distilled water (my original trainers taught me that). 4. If you can, use an oven, not water bath to heat. Same temp settings as on the water bath. 5. No metal at all. Use plastic hemostats and rinse them well between uses. 6. Mix your silver and methenemine first, then filter your sliver. Add the borax sol'n just before use, cut it down to 5ml. 7. The glomeruli take a long while to get to the point where they start to turn and they have to be dark. Paper bag brown is the what we say. 8. Always use de-I after periodic acid. When I teach this stain to students, we sometimes use the oven and sometimes the water bath. The oven cuts down on the precip and we can agitate the slides better. The gold chloride and hypo will help with the precip, but wipe the back and sides of the slides well between each change. Be careful not to push the solution back onto the section. The new Carson's version uses 50 mL of methenamine silver (5% silver in 3% methenamine) and 5mL of Borax. It also uses 1% periodic. I was always taught that 0.5% would give me non-specific staining because it was not sensitive enough. I trained using the formulation you did, but we also microwaved. We were specifically animal where I trained, and I have the microwave procedure somewhere if you want. Also, others mentioned the procedure on stainsfile. It works as well. I taught that method for a few years as well. Good luck and keep me posted. Sincerely, Toysha N. Mayer, DHSc, MBA, HT (ASCP) Assistant Professor/Associate Program Director HTL Program School of Health Professions MD Anderson Cancer Center tnmayer at mdanderson.org 713-563-3481 wk 832-710-1837 cell ------------------------------ Message: 2 Date: Thu, 23 Sep 2021 19:49:52 +0000 From: "Hood, Jordan" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Jones' Methenamine Silver Stain for Basement Membranes of Kidney - Issues and Questions Message-ID: <48bd31f00b074079baca10d963e1bc2f at med.umich.edu> Content-Type: text/plain; charset="iso-8859-1" Hello, I'm new to histology (and new to histonet), and I work in a small histology lab specializing in animal tissues that receives requests/submissions from researchers. I tried (and failed) to perform a Jones' Methenamine Silver stain on a client's submission of pig kidneys (formalin-fixed, paraffin-embedded, cut at 2.5 microns), and I need some help troubleshooting this stain since my co-workers are stumped, too. I used the following procedure from Rowley Biochemical: ~~~~~ "Fixation: 10% Buffered Neutral Formalin (F-113) or Bouin's Solution (F-40) or Zenker's (F-155) Sections: Paraffin, 2 microns Procedure: Acid washed glassware must be used!!!! 1. Deparaffinize and hydrate to distilled water. 2. Oxidize in Periodic Acid 0.5% (F-396-1) 11 minutes. Wash in chloride-free water. 3. Prepare Methenamine Silver solution by mixing: 42.5 ml Methenamine 3% (F-396-2), 2.5 ml Silver Nitrate, 5% (F-396-3) and 12.0 ml Borate Buffer, pH 8.2 (F-396-4). 4. Place slides in the solution and the entire jar in a water bath at 70?C for approx. 60-75 minutes. Check under microscope when slides appear medium brown microscopically. Every 10 minutes, once the medium brown color has been established, rinse a slide in 70?C, chloride free water and check under a microscope. Rinse again in hot water and return to the hot staining solution. As the staining time approaches the end point, check the slides, as above, every 1-2 minutes. The entire procedure must be performed quickly to prevent an uneven staining of the tissues. The slides should exhibit a brownish- yellow background, intense black reticulum fibers, and black basement membranes. If the slides become oversaturated, i.e. too black, destain in a dilute Potassium Ferricyanide Solution (F-396-11) for one or two dips. 5. Rinse well in distilled water. Tone in Gold Chloride 0.2% (F-396-5), 1 minute. If sections are overtoned place in Sodium Metabisulfite, 3% (F-396-12) for 1-3 minutes. Rinse well in distilled water. 6. Sodium Thiosulfate 3% (F-396-9), 1-2 miutes. Wash in running tap water, 10 minutes. Rinse well in distilled water. 7. Stain in Harris' Hematoxylin (F-396-6) containing 2-4ml of Glacial Acetic Acid per 100 ml for 5-15 minutes. Wash in water. 8. Differentiate in Acid Alcohol 1% (F-396-13) until the sections turn red. 9. Blue section in Ammonia Water, 0.3% (F-396-14). Wash thoroughly. 10. Counterstain in Eosin Y, 1%, Alcoholic Solution (F-396-7). 11. Dehydrate in 95% alcohol, absolute alcohol and clear in xylene 3 changes each. Mount. Stain Results: Basement membranes, reticulum fibers: Black Nuclei: Blue Cytoplasm, collagen, connective tissue: Pink-orange References: Jones, D.B., Amer.J.Path. 27:99 (1951). AFIP Manual of Histolocical Staining Methods, 3rd ed., Ed. L. Luna: NY: McGraw-Hill Publications, c. 1968, p. 97." ~~~~~ It became apparent that something went wrong during Step 4 when the slides were in the glass container (not a coplin jar - we have ten slides that we need to stain so we're using a rectangular glass container that holds ten slides on their sides - it does require a metal handle to move, but the handle is flexible and easy to remove after the glass slide rack has been transferred between containers) of silver solution in the water bath because there was lots of precipitate on the slides and floating on the surface of the silver solution. In my first test, I used five test slides (extra slides that we cut from the same blocks that were submitted to us). I deparaffinized them in coplin jars (moving them with plastic forceps) and hydrated them to deionized water. I transferred the slides to a glass slide rack that holds ten slides on their sides, added five blank slides that were rinsed in deionized water (so that the displacement of reagents would be equivalent to when we stain our ten "real" slides after testing is complete), and completed Step 2. I don't recall exactly how long the glass container of silver solution and the glass container of deionized water had been heating up in the water bath, but I would estimate ~15-30 minutes. The thermometer said that the water in the bath (not inside the containers) reached ~60-65 degrees Celsius. The silver solution was clear and colorless when I made it up, but by the time I put the slides into the warm silver solution, the solution was beginning to turn a light brown color (though it was still clear and I did not see any precipitate floating around). I removed the metal handle of the glass slide rack after the rack was transferred into the silver solution, but the metal handle did dip into the silver solution briefly. At some point, I noticed precipitate floating around of the surface of the silver solution. After ~80 minutes, I used plastic forceps to remove one test slide from the warm silver solution, dipped it several times into the warm deionized water to rinse it, and wiped off the back of the slide with gauze. The amount of precipitate was so extreme that the gauze did nearly nothing. I showed the slide to one of our pathologists and they could hardly see beyond the precipitate, but said that they couldn't see any staining of the structure that they were looking for (I forget exactly what it was, but I know it's supposed to turn black). In my second test (to see if the metal holder was the problem) that I performed immediately after the first test, I used one test slide. I deparaffinized it in the same coplin jars as before (moving it with plastic forceps) and hydrated it to deionized water. I used new glass containers for the periodic acid and deionized water rinse in Step 2, for making the silver solution in Step 3, and for the warm silver solution and warm deionized water in Step 4. I used plastic forceps to move the slide into the periodic acid, and propped it up in the container so that no glass rack or metal handle was used at all. I used plastic forceps to transfer the slide to the deionized water rinse, and dunked it several times and swished the slide around a bit. I used plastic forceps to transfer the slide into the warm(-ish) silver solution and propped it up against the side again. After approximately 20 minutes, I saw precipitate floating around, and I used plastic forceps to remove the slide from the silver solution. I dipped the slide into the warm(-ish) deionized water several times, and saw that the precipitate was again covering the slide and the tissue so I stopped there for the day. We purchased all of the reagents listed in the above procedure from Rowley Biochemical (except for the Glacial Acetic Acid mentioned in Step 7, but I didn't even get that far). Questions: 1. Could this indicate that the acid-washing was not done correctly? I made up a ~1% Hydrochloric Acid solution (with deionized water) and filled a plastic bin with the solution (I rinsed the bin with deionized water first). I then submerged all glassware (in several batches) for at least five minutes, then rinsed well with deionized water (not by filling a bin - I just used the hose of deionized water in our lab sink and poured it over the glassware) and left them to air-dry overnight. 2. Are using acid-washed glassware and avoiding metal even necessary precautions after the sodium thiosulphate in Step 6? I read that sodium thiosulphate "stops the reaction," and the procedure stops specifically saying to use deionized water after Step 6 and starts saying to use just "water" or "tap water." My lab refers to our waters as either "tap" or "deionized," so I'm assuming that using my deionized water is fine when the procedure calls for "distilled" or "dechlorinated." I don't even know enough to ask more questions, but I'm sure many more will arise after I test the stain again next week, so I welcome any and all advice about silver stains, acid-cleaning glassware, and literally anything else... Thank you!!! Jordan H. University of Michigan Ann Arbor, MI ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues ------------------------------ The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From c.tague at pathologyarts.com Tue Oct 12 10:14:54 2021 From: c.tague at pathologyarts.com (Curt Tague) Date: Tue, 12 Oct 2021 15:14:54 +0000 Subject: [Histonet] reprocessing tissue Message-ID: I have a problem... some tissue got processed very poorly, there was water in the system somewhere and a few blocks just look burnt.. the nuclei are faint and cloudy, no detail at all. I've tried the process of rehydrating with the 30% formaldehyde, glycerol and sodium acetate solution but they still process poorly, come out very brittle and just don't look good under the scope. Does anyone have a magic bullet to salvage these specimens? I can send a pic directly if it helps. Thanks, Curt From Jose.R.deGuzman at gunet.georgetown.edu Tue Oct 12 13:48:42 2021 From: Jose.R.deGuzman at gunet.georgetown.edu (De Guzman, Jose V) Date: Tue, 12 Oct 2021 18:48:42 +0000 Subject: [Histonet] reprocessing tissue In-Reply-To: References: Message-ID: Are these FFPE tissue? We usually just do the program in reverse order by deparaffinizing, 100% alc., 95% alc., 70% alc., water, formalin. Jose -----Original Message----- From: Curt Tague Sent: Tuesday, October 12, 2021 11:15 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] reprocessing tissue I have a problem... some tissue got processed very poorly, there was water in the system somewhere and a few blocks just look burnt.. the nuclei are faint and cloudy, no detail at all. I've tried the process of rehydrating with the 30% formaldehyde, glycerol and sodium acetate solution but they still process poorly, come out very brittle and just don't look good under the scope. Does anyone have a magic bullet to salvage these specimens? I can send a pic directly if it helps. Thanks, Curt ---------------------------------------------------------------------- MedStar Health is a not-for-profit, integrated healthcare delivery system, the largest in Maryland and the Washington, D.C., region. Nationally recognized for clinical quality in heart, orthopedics, cancer and GI. IMPORTANT: This e-mail (including any attachments) may contain information that is private, confidential, or protected by attorney-client or other privilege. If you received this e-mail in error, please delete it from your system without copying it and notify sender by reply e-mail, so that our records can be corrected... Thank you. Help conserve valuable resources - only print this email if necessary. From Sandra.Etheridge at gov.bc.ca Tue Oct 12 16:19:10 2021 From: Sandra.Etheridge at gov.bc.ca (Etheridge, Sandra AFF:EX) Date: Tue, 12 Oct 2021 21:19:10 +0000 Subject: [Histonet] Reprocessing Protocol Message-ID: Hi Curt, We have used Taggart's Method quite successfully in the past for poorly processed tissues. You can find it online. The isotonic saline may help to rehydrate your tissues. 1. Melt down the tissue block in the embedding centre block tray area and gently blot off the excess wax. Place the tissue in a newly labelled cassette. 2. Place the cassette into a beaker of isotonic saline (0.9% sodium chloride) and place it in the 65 C incubator/oven for one hour. This will melt the residual wax which will rise to the surface of the saline. 3. Remove the cassette from the saline, drain briefly and place in your processor from formalin, using a schedule that would have been of appropriate length as used initially. 4. Embed and section as per usual. Good luck! Sandra Etheridge -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: October 12, 2021 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 215, Issue 8 [EXTERNAL] This email came from an external source. Only open attachments or links that you are expecting from a known sender. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://can01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Csandra.etheridge%40gov.bc.ca%7Cbdb6ae0c78de43df498d08d98da1c4da%7C6fdb52003d0d4a8ab036d3685e359adc%7C0%7C0%7C637696548191633375%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=BrEkw92hcrZCE2E7LfNRrM3Zi%2BIxWyNUSevhTPcXqdQ%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. reprocessing tissue (Curt Tague) ---------------------------------------------------------------------- Message: 1 Date: Tue, 12 Oct 2021 15:14:54 +0000 From: Curt Tague To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] reprocessing tissue Message-ID: Content-Type: text/plain; charset="us-ascii" I have a problem... some tissue got processed very poorly, there was water in the system somewhere and a few blocks just look burnt.. the nuclei are faint and cloudy, no detail at all. I've tried the process of rehydrating with the 30% formaldehyde, glycerol and sodium acetate solution but they still process poorly, come out very brittle and just don't look good under the scope. Does anyone have a magic bullet to salvage these specimens? I can send a pic directly if it helps. Thanks, Curt ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://can01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Csandra.etheridge%40gov.bc.ca%7Cbdb6ae0c78de43df498d08d98da1c4da%7C6fdb52003d0d4a8ab036d3685e359adc%7C0%7C0%7C637696548191633375%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=BrEkw92hcrZCE2E7LfNRrM3Zi%2BIxWyNUSevhTPcXqdQ%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 215, Issue 8 **************************************** From jkiernan at uwo.ca Wed Oct 13 13:55:23 2021 From: jkiernan at uwo.ca (John Kiernan) Date: Wed, 13 Oct 2021 18:55:23 +0000 Subject: [Histonet] Reprocessing Protocol In-Reply-To: References: Message-ID: It makes no sense to heat a paraffin-infiltrated specimen in saline. Sodium chloride isn't soluble in hot paraffin or in any of the organic solvents used in tissue processing. Heating in water may melt and float out all the wax and rehydrate a specimen, but does it matter if some wax remains? Reprocessing is an attempt to correct the effects of incomplete dehydration. This can be done by taking the specimen back into the clearing agent (xylene or similar) and then into 2 changes of 100% alcohol (methyl, ethyl or isopropyl). Why rehydrate? For a rehydrated specimen, why go "from formalin, using a schedule that would have been of appropriate length as used initially"? This will surely produce, again, a block that is incompletely dehydrated. John Kiernan London, Canada = = = ________________________________ From: Etheridge, Sandra AFF:EX via Histonet Sent: October 12, 2021 5:19 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Reprocessing Protocol Hi Curt, We have used Taggart's Method quite successfully in the past for poorly processed tissues. You can find it online. The isotonic saline may help to rehydrate your tissues. 1. Melt down the tissue block in the embedding centre block tray area and gently blot off the excess wax. Place the tissue in a newly labelled cassette. 2. Place the cassette into a beaker of isotonic saline (0.9% sodium chloride) and place it in the 65 C incubator/oven for one hour. This will melt the residual wax which will rise to the surface of the saline. 3. Remove the cassette from the saline, drain briefly and place in your processor from formalin, using a schedule that would have been of appropriate length as used initially. 4. Embed and section as per usual. Good luck! Sandra Etheridge -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: October 12, 2021 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 215, Issue 8 [EXTERNAL] This email came from an external source. Only open attachments or links that you are expecting from a known sender. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://can01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Csandra.etheridge%40gov.bc.ca%7Cbdb6ae0c78de43df498d08d98da1c4da%7C6fdb52003d0d4a8ab036d3685e359adc%7C0%7C0%7C637696548191633375%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=BrEkw92hcrZCE2E7LfNRrM3Zi%2BIxWyNUSevhTPcXqdQ%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. reprocessing tissue (Curt Tague) ---------------------------------------------------------------------- Message: 1 Date: Tue, 12 Oct 2021 15:14:54 +0000 From: Curt Tague To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] reprocessing tissue Message-ID: Content-Type: text/plain; charset="us-ascii" I have a problem... some tissue got processed very poorly, there was water in the system somewhere and a few blocks just look burnt.. the nuclei are faint and cloudy, no detail at all. I've tried the process of rehydrating with the 30% formaldehyde, glycerol and sodium acetate solution but they still process poorly, come out very brittle and just don't look good under the scope. Does anyone have a magic bullet to salvage these specimens? I can send a pic directly if it helps. Thanks, Curt ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://can01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Csandra.etheridge%40gov.bc.ca%7Cbdb6ae0c78de43df498d08d98da1c4da%7C6fdb52003d0d4a8ab036d3685e359adc%7C0%7C0%7C637696548191633375%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=BrEkw92hcrZCE2E7LfNRrM3Zi%2BIxWyNUSevhTPcXqdQ%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 215, Issue 8 **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PKRichar at gundersenhealth.org Wed Oct 13 15:36:32 2021 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Wed, 13 Oct 2021 20:36:32 +0000 Subject: [Histonet] tissue disposal Message-ID: I am interested to learn how others are handling tissue disposal. How long to you keep wet tissue? Are you reusing containers? We currently dispose of the tissue and wash the containers and I am questioning if there is a better system? Best Wishes Pam ~ National Histology Professionals Day 3/10/21 Pathologists' Assistant Day 4/14/2021 Medical Laboratory Professionals Week April 18-24, 2021 National Cytotechnology Day 5/13/2021 +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org From tpodawiltz at yahoo.com Wed Oct 13 17:08:36 2021 From: tpodawiltz at yahoo.com (Thomas Podawiltz) Date: Wed, 13 Oct 2021 22:08:36 +0000 (UTC) Subject: [Histonet] tissue disposal In-Reply-To: References: Message-ID: <1337843513.2818844.1634162916174@mail.yahoo.com> We dumped tissue two after the final report is issued and toss the containers. Sent from Yahoo Mail for iPad On Wednesday, October 13, 2021, 3:36 PM, Richardson, Pam K via Histonet wrote: I am interested to learn how others are handling tissue disposal. How long to you keep wet tissue? Are you reusing containers? We currently dispose of the tissue and wash the containers and I am questioning if there is a better system? Best Wishes Pam ~ National Histology Professionals Day 3/10/21 Pathologists' Assistant Day 4/14/2021 Medical Laboratory Professionals Week April 18-24, 2021 National Cytotechnology Day 5/13/2021 +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Wed Oct 13 17:15:51 2021 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 13 Oct 2021 22:15:51 +0000 Subject: [Histonet] tissue disposal In-Reply-To: References: Message-ID: <662591041a27428eb37fd178f46cf931@SVDCMBX-MEX024.nswhealth.net> Hi Pam, We have used the Milestone Tissue Safe Plus unit with Integrated Data Logger card reader to seal formalin-fixed placentas (and other specimens if needed) since 2019. Placentas are drained of excess formalin in a chemical hood, placed into a labelled bag and sealed. So possibly for long term storage, the Tissue Safe Plus might be worth looking at. To save the environment, we try and re-cycle the larger plastic containers. As clean as possible but we try to not spend too much time on them. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Richardson, Pam K via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 14 October 2021 7:37 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] tissue disposal I am interested to learn how others are handling tissue disposal. How long to you keep wet tissue? Are you reusing containers? We currently dispose of the tissue and wash the containers and I am questioning if there is a better system? Best Wishes Pam ~ National Histology Professionals Day 3/10/21 Pathologists' Assistant Day 4/14/2021 Medical Laboratory Professionals Week April 18-24, 2021 National Cytotechnology Day 5/13/2021 +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://secure-web.cisco.com/1NGyXQUNr0j3MPQIjvmHqDwbUHBOGTSAJGY8v49mWsvp1_J2JSv0VL0YU2v5fBMwm9P9gkTaqYKmqnmP-QuNOClh6woo0CZiF0odkeVPTcoU5KS5ryxDxp16U_vnAsVLijWu1CwBGDPkJLePBE4OKDeXzL3cX-bzf0r-dKm0B9hw9e_yFECTUr-x3L_AVKV3OEhuj1kxg-ohIeIRtgqr4e0OlWI1MflK2moiDwp3XHYOx1kk8j4WPwEsuQqG1sLi6FKEDmyeUND1yNAGvWc5mhpyyyEHi9-3_9hd-8JUIk379SYu2qAHtKWCIdXHaovxUfgrnfqQp4X8G2t6pER6kt9RMNNAg7H_Kp9rl_V_SspLOu3QVAddkglNqSBHC3oFxLtxU5-HyiKOdHf5WqYOXdldkvybWkSXOf78Vl0gzJyIE4_lFYSwx26nqcd78P3vMVctVFNiNZry0Yo0oU6m-TjQlve8-a6kR98wk7nR04dQ/http%3A%2F%2Fwww.gundersenhealth.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From katherine at ka-recruiting.com Wed Oct 13 19:13:55 2021 From: katherine at ka-recruiting.com (Katherine Marano) Date: Wed, 13 Oct 2021 20:13:55 -0400 Subject: [Histonet] New opening Message-ID: Hi Histonetters, I just got in an exciting new job opportunity! I am working with a lab in Southern Connecticut that is looking to hire a *Manager of Anatomic Pathology, Molecular Pathology, and Digital Pathology*. My client needs someone who understands these three areas and who can support the transition in the lab from only derm to doing more. It is a permanent, full-time position. If you are interested, could you send me a resume and a good time for me to give you a call? If not, feel free to forward my info along to anyone you think could be a good fit! Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From LRaff at uropartners.com Thu Oct 14 07:23:00 2021 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 14 Oct 2021 12:23:00 +0000 Subject: [Histonet] thinking about why the lab is so important Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF17E4A444@COLOEXCH01.uropartners.local> Today's blog post: https://www.chicagonow.com/downsize-maybe/2021/10/paper-clips/ Lester J. Raff, MD MBA FCAP Laboratory Director UroPartners LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 Telephone 708-486-0076 Fax 708-492-0203 From amosbrooks at gmail.com Sun Oct 17 09:56:19 2021 From: amosbrooks at gmail.com (Amos Brooks) Date: Sun, 17 Oct 2021 10:56:19 -0400 Subject: [Histonet] Jones problems In-Reply-To: <3cb0bc06b6654d4a980b6984cdc27e62@med.umich.edu> References: <3cb0bc06b6654d4a980b6984cdc27e62@med.umich.edu> Message-ID: Hi, I think your silver precipitation issue could be cured by the thiosemicarbizide step too. If the reaction is sped up by this, you won't need to have the slides in long enough to develop the precipitate. That said, since it is a silver stain, you still need the glassware to be acid cleaned, but what you've been doing is probably just fine. I'm pretty sure this will solve your problem. Keep us posted though. Cheers, Amos On Thu, Oct 7, 2021, 12:57 PM Hood, Jordan wrote: > Hi Amos, > > > > It definitely helps, thank you! I brought this to my pathologist and she > said that it makes complete sense. I plan on ordering some TSC in solid > form. > > > > Is there a chance that I didn?t do the acid-washing of the glassware > correctly, leading to contamination? Or could all of the precipitate > floating around on the warm silver solution and on the slides be due to the > instability of the silver solution? > > > > Thank you so much! > > Jordan > > > > *From:* Amos Brooks > *Sent:* Saturday, September 25, 2021 8:03 AM > *To:* Hood, Jordan ; > histonet at lists.utsouthwestern.edu > *Subject:* Jones problems > > > > *External Email - Use Caution * > > Hi, > > When I first did this stain, I had really light staining of the GBM. > StainsFile has a note in the procedure that describes thiosemicarbizide. > Being a complete nerd, I wanted to see another source as well. Here is a > link to an article where it is used... > > https://pubmed.ncbi.nlm.nih.gov/2482996/ > > I also make these chemicals freshly each time. I accomplish this by > pre-weighing the solids sufficient for a 50ml volume and storing it in an > eppendorf tube. When you need it, pop the tube into the requirement volume > of water and you're golden. > > I do this because in addition to having good freshly made solutions, > I found the thiosemicarbizide goes bad rather quickly after use. TSC is the > lynch pin for the procedure. The silver on it's own does not react very > strongly with the aldehydes that are reduced by the periodic acid without > the TSC to facilitate the precipitation. > > > > I hope this helps, > > Amos Brooks > > ********************************************************** > Electronic Mail is not secure, may not be read every day, and should not > be used for urgent or sensitive issues > From criley at udel.edu Mon Oct 18 08:03:01 2021 From: criley at udel.edu (Charles Riley) Date: Mon, 18 Oct 2021 09:03:01 -0400 Subject: [Histonet] Frozen sectioning bones Message-ID: I am starting an all new research center and the researchers are looking to do frozen sections on bones. I have never done this before and would like some tips/suggestions. 1. What is/are the best cryostats to use for cutting bones if any? 2. What is the best process to embedding/cutting these specimens? From ehughes at invicro.com Tue Oct 19 10:35:57 2021 From: ehughes at invicro.com (Erik Hughes) Date: Tue, 19 Oct 2021 15:35:57 +0000 Subject: [Histonet] Temp HT's needed Message-ID: Good morning Histonet, Invicro is in need of two temp HT's at our Aliso Viejo CA lab. We are a CRO that does a lot of interesting projects so your days will never be the same. The daily work will be general Histology and IHC. I need people that are comfortable with the Leica Bond RX, Ventana Ultra & Discovery. These positions will be thru March 2022 at least and could move to fulltime with opportunities for advancement. Please reach out to me to discuss if there is interest, thank you. Take Care Erik Erik Hughes | Director of Lab Operations, Advanced Pathology Services Invicro, A Konica Minolta Company 1 Enterprise, Aliso Viejo, CA 92656 Cell: (970) 817-3097 www.invicro.com | ehughes at invicro.com This message contains confidential information and is intended only for the specified individual(s) named. If you are not an addressee, it is strictly forbidden to copy or share any part of this message with a third party without written consent of the sender. Please notify the sender immediately if you have inadvertently received this message and delete it from all your email-enabled devices. From relia1 at earthlink.net Wed Oct 20 07:47:27 2021 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 20 Oct 2021 08:47:27 -0400 Subject: [Histonet] Tips for Self-Care at work - What a great read. And Histology Career opportunities you want to check out. Message-ID: <000001d7c5b0$a3e8fbf0$ebbaf3d0$@earthlink.net> Hello Histopeeps, I just read this article online and had to share it! With the nationwide histotech shortage at a more critical stage than ever you Histopeeps my histopeep need to stay strong and take care of yourself. You can?t take care of others if you don?t take care of yourself. This is a great read on ?Self-Care Activities to reduce burnout at work. If you have time to read it I would love to know what you think! https://www.mscareergirl.com/self-care-activities-to-reduce-burnout-at-work/ If you or anyone you know might be in the market for a new opportunity The compensation has never been better. The opportunity to take on more responsibility has never been better. The relocation/bonus packages have never been better. And, Histopeeps!! I have some amazing job opportunities in: ? California ? Florida ? Texas ? Tennessee ? Colorado ? Illinois And New Opportunities coming in on a daily basis!! All of these clients are offering full time permanent positions with excellent compensation packages including VERY competitive pay rates, fantastic benefits, relocation and/or sign on bonuses and a great team to work with!! I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. ? So if you think you or someone you know might be interested please contact me. We can look at these positions or let?s talk about where you want to go! I can be reached at 866-607-3542, on my cell at 407-353-5070 or relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! To unsubscribe please reply to this email with ?unsubscribe? From relia1 at earthlink.net Thu Oct 21 11:18:45 2021 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 21 Oct 2021 12:18:45 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin 10/21/2021 Tips for Self Care at work: A great read and some exciting new opportunities with starting bonuses up to 15K Message-ID: <000001d7c697$53373f90$f9a5beb0$@earthlink.net> Hi Histopeeps! I just read this article online and had to share it! With the nationwide histotech shortage at a more critical stage than ever self-care has never been more important. You can't take care of others if you don't take care of yourself. This is a great read on "Self-Care Activities to reduce burnout at work. If you have time to read it I would love to know what you think! https://www.mscareergirl.com/self-care-activities-to-reduce-burnout-at-work/ If you or anyone you know might be in the market for a new opportunity. The compensation HAS NEVER BEEN BETTER! The opportunity to take on more responsibility HAS NEVER BEEN BETTER! The relocation/bonus packages HAVE NEVER BEEN BETTER!! And, I have some amazing job opportunities in: California Florida Texas Tennessee Colorado Illinois New York New Jersey And New Opportunities coming in on a daily basis!! All of these clients are offering full time permanent positions with excellent compensation packages including VERY competitive pay rates, fantastic benefits, relocation and/or sign on bonuses and a great team to work with!! I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. We can look at these positions or let's talk about where you want to go! I can be reached at 866-607-3542, on my cell at 407-353-5070 or relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From relia1 at earthlink.net Thu Oct 21 12:04:08 2021 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 21 Oct 2021 13:04:08 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin 10/21/2021 Tips for Self Care at work: A great read and some exciting new opportunities with starting bonuses up to 15K Message-ID: <000001d7c69d$a9c69d50$fd53d7f0$@earthlink.net> Hi Histopeeps! I just read this article online and had to share it! With the nationwide histotech shortage at a more critical stage than ever self-care has never been more important. You can't take care of others if you don't take care of yourself. This is a great read on "Self-Care Activities to reduce burnout at work. If you have time to read it I would love to know what you think! https://www.mscareergirl.com/self-care-activities-to-reduce-burnout-at-work/ If you or anyone you know might be in the market for a new opportunity. The compensation HAS NEVER BEEN BETTER! The opportunity to take on more responsibility HAS NEVER BEEN BETTER! The relocation/bonus packages HAVE NEVER BEEN BETTER!! And, I have some amazing job opportunities in: California Florida Texas Tennessee Colorado Illinois New York New Jersey And New Opportunities coming in on a daily basis!! All of these clients are offering full time permanent positions with excellent compensation packages including VERY competitive pay rates, fantastic benefits, relocation and/or sign on bonuses and a great team to work with!! I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. We can look at these positions or let's talk about where you want to go! I can be reached at 866-607-3542, on my cell at 407-353-5070 or relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From THuynh at mdanderson.org Fri Oct 22 13:20:49 2021 From: THuynh at mdanderson.org (Huynh,Thomas) Date: Fri, 22 Oct 2021 18:20:49 +0000 Subject: [Histonet] [EXT] Histonet Digest, Vol 215, Issue 16 In-Reply-To: References: Message-ID: Hello Administrator, I would like to ask if it is okay or allow to post jobs on the histonet site? I have a friend who is in desperate need of help. Thanks, Thomas Thomas Huynh, BS, HT(ASCP) CM Chief Histology Laboratory Department of Veterinary Medicine & Surgery UT MD Anderson Cancer Center 1515 Holcombe Unit 063 THuynh at mdanderson.org T 713-745-5077 Lab 713-792-2793 C 832-794-0741 True Colors: Gold -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Friday, October 22, 2021 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXT] Histonet Digest, Vol 215, Issue 16 WARNING: This email originated from outside of MD Anderson. Please validate the sender's email address before clicking on links or attachments as they may not be safe. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PfbeBCCAmug!034AOEtqBSB9JjzBTQzrgMy7HGZE4e3JivpZKpKN5l1xXYyLCZZJ2TnM3TF0D4jA$ or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From thomas6093 at yahoo.com Sun Oct 24 09:42:16 2021 From: thomas6093 at yahoo.com (Thomas Huynh) Date: Sun, 24 Oct 2021 14:42:16 +0000 (UTC) Subject: [Histonet] Histology Jobs Houston, TX References: <1278571686.377822.1635086536306.ref@mail.yahoo.com> Message-ID: <1278571686.377822.1635086536306@mail.yahoo.com> Hello Histonetter, I am posting this for a friend:? We are a private reference laboratory that is derm focused with 3 full time histotech?openings,?3 PRN histotech openings, 1 PRN transcriptionist opening, and 1 PRN grossing opening.? The?full time histotech?openings have shifts of?11pm- 7:30am, 12am- 8:30am, and?3:30am - 12pm. PRN histotech?shifts vary for when coverage is needed, Monday through Saturday.? Our?PRN transcriptionist shift varies based on need and has a start time of?5pm, Tuesday through Friday.? Our PRN grossing position has a start time of 10am and 2pm.??All shifts include a shift differential of $3/hr from 10pm - 5am.? Thanks,Thomas From kathryn.perkinson at duke.edu Mon Oct 25 08:01:26 2021 From: kathryn.perkinson at duke.edu (Kathryn Perkinson) Date: Mon, 25 Oct 2021 13:01:26 +0000 Subject: [Histonet] Histonet Digest, Vol 215, Issue 18 In-Reply-To: References: Message-ID: Open Position: Laboratory Supervisor Histology which is part of the Division of Anatomic Pathology and Digital Analytics. This position offers an opportunity to supervise a large laboratory with 3 shifts and weekends. It is part of a larger division which includes IHC, FISH, and Digital Imaging. In this position, you have room to learn new technologies and become part of a dynamic team. Please contact me if you have 5 years Histology experience and a BS degree and HT or HTL certification and supervisory experience (preferred not required). Send your resume today to get started on a new role! Kathryn R. Perkinson, BS, HTL(ASCP), CSSGB Manager, Division of Anatomic Pathology and Digital Analytics Box 3712, Room 4710 Duke South Clinic Building Duke University Medical Center Durham, NC 27710 Office: 919-684-5822 Cell: 919-603-7672 -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Sunday, October 24, 2021 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 215, Issue 18 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!OToaGQ!6PMpgI1jz4K3oUc5rBIIq3ZbAow6DL__aJ7AM4KSraakh4MbyCEZhdtqfqoCGu9PpkOcQTc$ or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Histology Jobs Houston, TX (Thomas Huynh) ---------------------------------------------------------------------- Message: 1 Date: Sun, 24 Oct 2021 14:42:16 +0000 (UTC) From: Thomas Huynh To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Histology Jobs Houston, TX Message-ID: <1278571686.377822.1635086536306 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Hello Histonetter, I am posting this for a friend:? We are a private reference laboratory that is derm focused with 3 full time histotech?openings,?3 PRN histotech openings, 1 PRN transcriptionist opening, and 1 PRN grossing opening.? The?full time histotech?openings have shifts of?11pm- 7:30am, 12am- 8:30am, and?3:30am - 12pm. PRN histotech?shifts vary for when coverage is needed, Monday through Saturday.? Our?PRN transcriptionist shift varies based on need and has a start time of?5pm, Tuesday through Friday.? Our PRN grossing position has a start time of 10am and 2pm.??All shifts include a shift differential of $3/hr from 10pm - 5am.? Thanks,Thomas ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!OToaGQ!6PMpgI1jz4K3oUc5rBIIq3ZbAow6DL__aJ7AM4KSraakh4MbyCEZhdtqfqoCGu9PpkOcQTc$ ------------------------------ End of Histonet Digest, Vol 215, Issue 18 ***************************************** From relia1 at earthlink.net Tue Oct 26 11:07:23 2021 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 26 Oct 2021 12:07:23 -0400 Subject: [Histonet] Histonetters!!! The Florida Society for Histotechnology is having their Fall Meeting virtually. Here is the info. Message-ID: <000501d7ca83$908b6520$b1a22f60$@earthlink.net> Hello Histopeeps, I hope you are doing well. I just wanted to drop you a quick line and let you know that the Florida Society for Histotechnology is having their Fall Meeting. It is a one day virtual event on Saturday November 19th. This is could be an inexpensive and a great way to for you to get your ceus! And you will be supporting our state society. Here is the link for more information: https://fshgroup.org/meetinginfo.php?id=1 &ts=1635189663&fbclid=IwAR3ZHO-_pbokowCtGXoffVZXVPg4Bjv0weSw_7wGQ3lHhgKusQrN 86YI_2c If you or anyone you know is looking for a new opportunity, I have some awesome positions available in Florida. All of these positions are permanent full time positions and my clients offer very competitive compensation and perks! Here are the locations: Fort Myers Gainesville Sarasota Panama City I also have amazing opportunities in: Dallas, TX Abilene, TX Aurora, CO Long Island, NY NYC NJ locations Chicago, IL Aliso Viejo, CA San Diego, CA Wilmington, NC And new opportunities coming in DAILY!! If you or any of your friends would like more information on any of these positions or if you would like to discuss opportunities in other areas or future job searches please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or on my cell/text at 407-353-5070 or at relia1 at earthlink.net Remember I offer over 20 years of recruiting and employment counseling experience, knowledgeable, confidential and responsive service to you and your friends and a permanent placement practice dedicated to the histology profession. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember. It never hurts to keep an eye open, Even if you are happy in your present job. p.s. How are you celebrating Halloween? Happy Halloween!!!!!!!!!!!! Please let me know if there is anything I can do for you. Have a great week! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From amurvosh at advancederm.net Tue Oct 26 11:26:44 2021 From: amurvosh at advancederm.net (Anne Murvosh) Date: Tue, 26 Oct 2021 16:26:44 +0000 Subject: [Histonet] Stain line order Message-ID: When doing frozen section staining for Mohs we have DI water and don't need a clearing agent. I am helping set up someone who is using tap water and needs to clear it. They mentioned that they used to clear first, rinse, then use bluing. I thought it was the opposite after heme. Rinse, bluing, rinse, clearing. I don't remember which is first. Actually do you need a bluing if using tap water since that blues better then DI water? Thanks for your input, it's been 10 years since I had to think about this. Anne Anne Murvosh Histology Technician This electronic transmission and any documents accompanying this electronic transmission may contain information that is confidential and/or legally privileged. The information is intended only for the use of the individual or entity named above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on or regarding the contents of this electronically transmitted information is strictly prohibited. If you have received this e-mail in error, please notify the sender and delete this message immediately. HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return or destruction. From jmacdonald at mtsac.edu Tue Oct 26 11:58:44 2021 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Tue, 26 Oct 2021 16:58:44 +0000 Subject: [Histonet] Stain line order In-Reply-To: References: Message-ID: That step is known as differentiation. Clearing involves an organic solvent to prepare the slides for coverslipping in a synthetic mounting medium. A regressive H&E uses differentiation, usually with 1% acid alcohol. A progressive stain does not. Some companies use a modified progressive using clarifier which is an acid alcohol using acetic acid. -----Original Message----- From: Anne Murvosh Sent: Tuesday, October 26, 2021 9:56 AM To: Mac Donald, Jennifer Subject: RE: Stain line order EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Clear; the tap water makes a blue film on the slides. I think this person used to use HCL water. I used clarifier 2 in the past. Thanks Anne -----Original Message----- From: Mac Donald, Jennifer Sent: Tuesday, October 26, 2021 9:49 AM To: Anne Murvosh Subject: RE: Stain line order Clear or differentiate? -----Original Message----- From: Anne Murvosh via Histonet Sent: Tuesday, October 26, 2021 9:27 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Stain line order EXTERNAL SENDER- Exercise caution with requests, links, and attachments. When doing frozen section staining for Mohs we have DI water and don't need a clearing agent. I am helping set up someone who is using tap water and needs to clear it. They mentioned that they used to clear first, rinse, then use bluing. I thought it was the opposite after heme. Rinse, bluing, rinse, clearing. I don't remember which is first. Actually do you need a bluing if using tap water since that blues better then DI water? Thanks for your input, it's been 10 years since I had to think about this. Anne Anne Murvosh Histology Technician This electronic transmission and any documents accompanying this electronic transmission may contain information that is confidential and/or legally privileged. The information is intended only for the use of the individual or entity named above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on or regarding the contents of this electronically transmitted information is strictly prohibited. If you have received this e-mail in error, please notify the sender and delete this message immediately. HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return or destruction. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C4dab97c684334d4b097608d998a17b6f%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637708641600584572%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=sr3vBKDrB1vzDxN1r30xsGa%2FjxhBgy%2FrhOoHWnsqvaI%3D&reserved=0 From deewolfe at anatechltdusa.com Tue Oct 26 12:41:52 2021 From: deewolfe at anatechltdusa.com (Dee Wolfe) Date: Tue, 26 Oct 2021 13:41:52 -0400 Subject: [Histonet] Ada's Mail Stain line order In-Reply-To: References: Message-ID: Hi Anne, Are they using the term "clearing" loosely, but really mean "rinsing"? The term "clearing" typically refers to the step after the alcohol (dehydration) and before coverslipping (if using a permanent, solvent based mounting media). Not sure why someone would "clear" before the rinse and bluing step in frozen section staining. For paraffin embedded specimens, the slide would need to Deparaffinize (in clearing agent-xylene or xylene substitute), dehydrate (graded alcohols?high to low), water rinse (preferably DI), stain the nuclei (hematoxylin), water rinse, decolorize (acid alcohol-if staining regressively), water rinse(s), bluing, water rinses, graded alcohol?low to high, cytoplasmic staining (Eosin), alcohol rises, clearing. For frozen section: 100% alcohol (or other fixative), water rise, hematoxylin (usually progressive, so no need to decolorize), bluing, water rinse, etc? You may choose to skip bluing as tap water can do it. Although for consistency it should be kept in (tap water pH can with seasons, water treatment facilty processes, etc.) Dee Wolfe On Oct 26, 2021, at 12:26 PM, Anne Murvosh via Histonet wrote: > When doing frozen section staining for Mohs we have DI water and don't need a clearing agent. I am helping set up someone who is using tap water and needs to clear it. They mentioned that they used to clear first, rinse, then use bluing. I thought it was the opposite after heme. Rinse, bluing, rinse, clearing. I don't remember which is first. Actually do you need a bluing if using tap water since that blues better then DI water? Thanks for your input, it's been 10 years since I had to think about this. Anne > > > Anne Murvosh > Histology Technician > > > > This electronic transmission and any documents accompanying this electronic transmission may contain information that is confidential and/or legally privileged. The information is intended only for the use of the individual or entity named above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on or regarding the contents of this electronically transmitted information is strictly prohibited. If you have received this e-mail in error, please notify the sender and delete this message immediately. > > HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return > or destruction. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed Oct 27 08:43:23 2021 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 27 Oct 2021 09:43:23 -0400 Subject: [Histonet] The Florida Society for Histotechnology is holding a Fall Virtual Meeting on Saturday November 20th and Here is the info Message-ID: <004d01d7cb38$9cdaa1a0$d68fe4e0$@earthlink.net> Hello Histopeeps, I hope you are doing well. I just wanted to drop you a quick line and let you know that the Florida Society for Histotechnology is having their Fall Meeting. It is a one day virtual event on Saturday November 20th. This is could be an inexpensive and a great way to for you to get your ceus! And you will be supporting our state society. Here is the link for more information: https://fshgroup.org/meetinginfo.php?id=1 &ts=1635189663&fbclid=IwAR3ZHO-_pbokowCtGXoffVZXVPg4Bjv0weSw_7wGQ3lHhgKusQrN 86YI_2c If you or anyone you know is looking for a new opportunity, I have some awesome positions available in Florida. All of these positions are permanent full time positions and my clients offer very competitive compensation and perks! Here are the locations: Fort Myers Gainesville Sarasota Panama City And new opportunities coming in DAILY!! If you or any of your friends would like more information on any of these positions or if you would like to discuss opportunities in other areas or future job searches please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or on my cell/text at 407-353-5070 or at relia1 at earthlink.net Remember I offer over 20 years of recruiting and employment counseling experience, knowledgeable, confidential and responsive service to you and your friends and a permanent placement practice dedicated to the histology profession. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember. It never hurts to keep an eye open, Even if you are happy in your present job. p.s. How are you celebrating Halloween? Happy Halloween!!!!!!!!!!!! Please let me know if there is anything I can do for you. Have a great week! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! To unsubscribe please reply to relia1 at earthlink.net with unsubscribe. From tcampbell at fgamd.com Wed Oct 27 09:59:30 2021 From: tcampbell at fgamd.com (Campbell, Tasha) Date: Wed, 27 Oct 2021 14:59:30 +0000 Subject: [Histonet] cellblock Message-ID: <771d6312e68043649bd0279052b7f341@fgamd.com> What is the best collection fluid for FNAs for cellblock? I used to use Saccomano fluid at another lab, but we are bringing them in house at my current lab and the cytopath told me to have them do the needle rinse in formalin. Is formalin fine for that? Thanks! Tasha Campbell, B.S., HTL (ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 From csheil at jcu.edu Wed Oct 27 12:05:19 2021 From: csheil at jcu.edu (Sheil, Christopher) Date: Wed, 27 Oct 2021 13:05:19 -0400 Subject: [Histonet] request to post on Histonet Message-ID: Dear Histonet admin, I'm writing to ask if it is possible for me to post a recruiting message on Histonet. I am hoping to recruit an MS student into my lab next year for a project that involves histology of snake tissues. If it is possible for me to post a recruiting message on Histonet, I'll send specific information about the project, links to our grad studies office, and information about our MS program. Sincerely, -Chris Christopher A. Sheil Professor, Chair Department of Biology John Carroll University University Heights, OH 44118 Email: csheil at jcu.edu Phone: 216.397.3088 From John.Shelton at UTSouthwestern.edu Wed Oct 27 12:15:47 2021 From: John.Shelton at UTSouthwestern.edu (John Shelton) Date: Wed, 27 Oct 2021 17:15:47 +0000 Subject: [Histonet] request to post on Histonet In-Reply-To: References: Message-ID: Feel free Chris Hope you get the support you need in the Ohio area. Ssssnake histology - ssssounds cool John John M Shelton Core Operations Manager Histonet List Owner & Moderator UT Southwestern Histo Pathology Core 5323 Harry Hines Blvd Dallas, Texas 75390-8573 (214)648-1451 Visit the Core's website at: http://www.utsouthwestern.edu/labs/histo-pathology/ Visit Histonet Archives at: http://www.histosearch.com/histonet.html ________________________________ From: zzz_Tag_histonet-lists Sent: Wednesday, October 27, 2021 12:05 PM To: zzz_Tag_histonet-lists Subject: [Histonet] request to post on Histonet Dear Histonet admin, I'm writing to ask if it is possible for me to post a recruiting message on Histonet. I am hoping to recruit an MS student into my lab next year for a project that involves histology of snake tissues. If it is possible for me to post a recruiting message on Histonet, I'll send specific information about the project, links to our grad studies office, and information about our MS program. Sincerely, -Chris Christopher A. Sheil Professor, Chair Department of Biology John Carroll University University Heights, OH 44118 Email: csheil at jcu.edu Phone: 216.397.3088 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ UT Southwestern Medical Center The future of medicine, today. From portera at msu.edu Thu Oct 28 13:13:04 2021 From: portera at msu.edu (Porter, Amy) Date: Thu, 28 Oct 2021 18:13:04 +0000 Subject: [Histonet] Question for Research on Bone Message-ID: So I am working with Canine femurs and tibias that have been dissected as medial and lateral - they have been decaled in formic acid - rinsed in running tap well - processed on a routine 1 hour processing program ..... cutting very well!! My issue is that the articular cartilage on the bone surface keep flipping up and over onto itself - I am using a 37C slide warmer overnight to allow them to hopefully flatten out more than on the water bath and then drying in the 56C oven for about 1 hour - then staining on automated Leica stainer - they are intact at 37..... look like the might be curling at 56C?? Anyone have any suggestions??? TIA - Amy Amy S. Porter, HT (ASCP) - Lab Supervisor Michigan State University Investigative HistoPathology Lab 567 Wilson Road - Rm 2133 East Lansing, MI 48824 Ph: 517-884-5026 (she/her/hers) https://sites.google.com/msu.edu/ihpl/home "Life is like riding a bicycle. To keep your balance, you must keep moving." - Albert Einstein From Prabhakar.Ashwin at hcahealthcare.com Fri Oct 29 12:36:37 2021 From: Prabhakar.Ashwin at hcahealthcare.com (Ashwin Ashwin) Date: Fri, 29 Oct 2021 17:36:37 +0000 Subject: [Histonet] SAKURA XP 120 Message-ID: Hello Everyone, Do you have XP120 rapid tissue processor? If so, could you please let me know your usage protocol and any tips and trade for validation. I would appreciate your help with this. Thank you Prabhakar Ashwin Regional Director of Histology IRL Ft Lauderdale 5361 NW 33rd Ave, Fort Lauderdale, FL 33309 HCA | Hospital Corporation of America