From Katherine.Racine at aah.org Sat May 1 01:46:24 2021 From: Katherine.Racine at aah.org (Racine, Katherine) Date: Sat, 1 May 2021 06:46:24 +0000 Subject: [Histonet] Reprocessing biopsy tissue Message-ID: Hello, I am wondering if anyone has had any success with reprocessing biopsy tissue, and if so, what process you used. We recently had an issue where water was reintroduced into the tissue during the dehydration step due to an incorrect alcohol percentage being entered. The Peloris thought it was ending the dehydration step in a 100% alcohol but in reality was only a 83%. Multiple tissue types were affected including prostate, liver, and lung biopsies. Any insight would be greatly appreciated!! Thanks, Katherine Racine (histology supervisor) Aurora Baycare Medical Center Advocate Aurora Health Get Outlook for iOS From jmacdonald at mtsac.edu Mon May 3 00:07:55 2021 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Mon, 3 May 2021 05:07:55 +0000 Subject: [Histonet] Animal Histology Message-ID: Can anyone recommend a good book for processing animal tissue? Thanks, Jennifer From jkiernan at uwo.ca Mon May 3 12:31:54 2021 From: jkiernan at uwo.ca (John Kiernan) Date: Mon, 3 May 2021 17:31:54 +0000 Subject: [Histonet] Animal Histology In-Reply-To: References: Message-ID: Jennifer MacDonald asked, "Can anyone recommend a good book for processing animal tissue?" Here's a list of 14 of the ones published since 1990, with very brief descriptive notes. There are, of course, plenty of good books that are older than these. Lyon,H (1991): Theory and Strategy in Histochemistry. A Guide to the Selection and Understanding of Techniques. Springer-Verlag, Berlin. All on theory; no instructions. All aspects of preparation, staining etc are covered. 32 chapters, by different people; one bibliography. Reprinted 2011. ISBN 9783642737442. Kok,LP; Boon,ME (1992): Microwave Cookbook for Microscopists. Art and Science of Visualization. 3rd ed. Coulomb Press Leyden, Leiden. 432 pages. Plenty of microwave theory. Applications in fixation, infiltr, embedding, decalcif., many staining methods including conn.tiss., cytol., neuro. Enzyme, immuno- & hybridization histochemistry. Sanderson,JB (1994): Biological Microtechnique. (Microscopy Handbooks, 28.) BIOS Scientific Publications & Royal Microscopical Society, Oxford. 224 pages. Detailed paractical instructions, with rationale, & titled bibliography for each of the 7 chapters. Collection, Fixation, Processing, Microtomy, Other prep. methods, Staining, Finishing (mounting etc). Chayen,J; Bitensky,L (1991): Practical Histochemistry. 2nd ed. Wiley, Chichester. 321 pages. Special emphasis on use of unfixed cryostat sections. Presnell,JK; Schreibman,MP (1997): Humason's Animal Tissue Techniques. 5th ed. Johns Hopkins University Press, Baltimore. 572 pages. Humason's Animal Tissue Techniques, 5th edition. Includes chapters on immunohistochem, safety, microwaves, lab hints, suppliers, etc. Hayat,MA (1993): Stains and Cytochemical Methods. Plenum Press, New York. Book of techniques. Many are critically discussed. emphasis on EM, but plenty of LM too. Carson,FL; Hladik,C (2009): Histotechnology. A Self-Instructional Text. 3rd ed. American Society of Clinical Pathologists, Chicago. 400 pages. Freida Carson's textbook. 3rd ed, with Hladik, is 96 pages longer than 2nd (1997); with EM and cytopreparation; glossary. ISBN 9780891895817. (There is a 4th ed, 2015, which I haven't seen.) Kiernan,JA (2015): Histological and Histochemical Methods: Theory and Practice. 5th ed. Scion, Banbury, UK. 606 pages. Kumar,GL; Kiernan,JA (Eds.) (2010): Education Guide: Special Stains and H & E. 2nd ed. Dako North America, Carpinteria, CA. 300 pages. 33 chapters. Photos. PDF file is a free download from https://www.agilent.com/en/dako-pathology-education-guides/. Wick,MR (Ed.) (2008): Diagnostic Histochemistry. Cambridge University Press, New York. 460 pages. Multi-author book. Applications of techniques to path. diagnosis. Chapters by systems, organs, diseases etc. Emphasizes value, economy of pre-1970s (before immuno) histochemical methods. Only Ch1, pp.1-27 describes techniques. Many colour photos. Orchard,G; Nation,B (Eds.) (2012): Histopathology. Oxford University Press, Oxford, UK. 396 pages. Multi-author textbook book. Applications of techniques to path. diagnosis. Chapters mainly by methods, with applications to systems, organs, diseases. One of an Inst. of Biomed. Science series, with web site at www.oxfordtextbooks.co.uk/orc/fbs/. Mulisch,M; Welsch,U (Eds.) (2015): Romeis - Mikroskopische Technik. 19th ed. Springer-Verlag, Berlin. 603 pages. 25 chapters, 3 appendices. All types of microscopy, staining, histochem, etc. (in German) ISBN 9783642551901. Exbrayat,JM (Ed.) (2013): Histochemical and Cytochemical Nethods of Visualization. (Series Ed: Morel,G. Methods in Visualization.) CRC Press, Boca Raton, FL. 335 pages. 13 chapters, 10 contributors. 1-7 are for LM; 8-12 for EM; 13 on image quantification. ISBN 9781439822227. Suvarna,SK; Layton,C; Bancroft,JD (Eds.) (2018): Bancroft's Theory and Practice of Histological Techniques. 8th ed. Churchill Livingstone Elsevier, London. 672 pages. In this 8th ed., histochem chapters for lipids, proteins, nucleic acids, enzyme activities are now compressed into Appx I (of VIII). Hope this helps. For older books, look in AbeBooks, Amazon etc for ones by GG Brown, HC Cook, CFA Culling, RAB Drury, M Gabe, P Gray, RW Horobin, G Humason, JFA McManus, AGE Pearse. John A. Kiernan Dept of Anatomy & Cell Biology University of Western Ontario London, Canada = = = ________________________________ From: Mac Donald, Jennifer via Histonet Sent: May 3, 2021 1:07 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Animal Histology Can anyone recommend a good book for processing animal tissue? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster at umn.edu Mon May 3 12:34:01 2021 From: cforster at umn.edu (Colleen Forster) Date: Mon, 3 May 2021 12:34:01 -0500 Subject: [Histonet] Animal Histology In-Reply-To: References: Message-ID: Wow, what an excellent list John. I didn't ask the question but will surely use the response. Thank you for sharing. Respectfully, Colleen Forster HT(ASCP)QIHC University of Minnesota On Mon, May 3, 2021 at 12:32 PM John Kiernan via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Jennifer MacDonald asked, "Can anyone recommend a good book for processing > animal tissue?" Here's a list of 14 of the ones published since 1990, with > very brief descriptive notes. There are, of course, plenty of good books > that are older than these. > > Lyon,H (1991): Theory and Strategy in Histochemistry. A Guide to the > Selection and Understanding of Techniques. Springer-Verlag, Berlin. All on > theory; no instructions. All aspects of preparation, staining etc are > covered. 32 chapters, by different people; one bibliography. Reprinted > 2011. ISBN 9783642737442. > > Kok,LP; Boon,ME (1992): Microwave Cookbook for Microscopists. Art and > Science of Visualization. 3rd ed. Coulomb Press Leyden, Leiden. 432 pages. > Plenty of microwave theory. Applications in fixation, infiltr, embedding, > decalcif., many staining methods including conn.tiss., cytol., neuro. > Enzyme, immuno- & hybridization histochemistry. > > Sanderson,JB (1994): Biological Microtechnique. (Microscopy Handbooks, > 28.) BIOS Scientific Publications & Royal Microscopical Society, Oxford. > 224 pages. Detailed paractical instructions, with rationale, & titled > bibliography for each of the 7 chapters. Collection, Fixation, Processing, > Microtomy, Other prep. methods, Staining, Finishing (mounting etc). > > Chayen,J; Bitensky,L (1991): Practical Histochemistry. 2nd ed. Wiley, > Chichester. 321 pages. Special emphasis on use of unfixed cryostat sections. > > Presnell,JK; Schreibman,MP (1997): Humason's Animal Tissue Techniques. 5th > ed. Johns Hopkins University Press, Baltimore. 572 pages. Humason's Animal > Tissue Techniques, 5th edition. Includes chapters on immunohistochem, > safety, microwaves, lab hints, suppliers, etc. > > Hayat,MA (1993): Stains and Cytochemical Methods. Plenum Press, New York. > Book of techniques. Many are critically discussed. emphasis on EM, but > plenty of LM too. > > Carson,FL; Hladik,C (2009): Histotechnology. A Self-Instructional Text. > 3rd ed. American Society of Clinical Pathologists, Chicago. 400 pages. > Freida Carson's textbook. 3rd ed, with Hladik, is 96 pages longer than 2nd > (1997); with EM and cytopreparation; glossary. ISBN 9780891895817. (There > is a 4th ed, 2015, which I haven't seen.) > > Kiernan,JA (2015): Histological and Histochemical Methods: Theory and > Practice. 5th ed. Scion, Banbury, UK. 606 pages. > > Kumar,GL; Kiernan,JA (Eds.) (2010): Education Guide: Special Stains and H > & E. 2nd ed. Dako North America, Carpinteria, CA. 300 pages. 33 chapters. > Photos. PDF file is a free download from > https://www.agilent.com/en/dako-pathology-education-guides/. > > Wick,MR (Ed.) (2008): Diagnostic Histochemistry. Cambridge University > Press, New York. 460 pages. Multi-author book. Applications of techniques > to path. diagnosis. Chapters by systems, organs, diseases etc. Emphasizes > value, economy of pre-1970s (before immuno) histochemical methods. Only > Ch1, pp.1-27 describes techniques. Many colour photos. > > Orchard,G; Nation,B (Eds.) (2012): Histopathology. Oxford University > Press, Oxford, UK. 396 pages. Multi-author textbook book. Applications of > techniques to path. diagnosis. Chapters mainly by methods, with > applications to systems, organs, diseases. One of an Inst. of Biomed. > Science series, with web site at www.oxfordtextbooks.co.uk/orc/fbs/. > > Mulisch,M; Welsch,U (Eds.) (2015): Romeis - Mikroskopische Technik. 19th > ed. Springer-Verlag, Berlin. 603 pages. 25 chapters, 3 appendices. All > types of microscopy, staining, histochem, etc. (in German) ISBN > 9783642551901. > > Exbrayat,JM (Ed.) (2013): Histochemical and Cytochemical Nethods of > Visualization. (Series Ed: Morel,G. Methods in Visualization.) CRC Press, > Boca Raton, FL. 335 pages. 13 chapters, 10 contributors. 1-7 are for LM; > 8-12 for EM; 13 on image quantification. ISBN 9781439822227. > > Suvarna,SK; Layton,C; Bancroft,JD (Eds.) (2018): Bancroft's Theory and > Practice of Histological Techniques. 8th ed. Churchill Livingstone > Elsevier, London. 672 pages. In this 8th ed., histochem chapters for > lipids, proteins, nucleic acids, enzyme activities are now compressed into > Appx I (of VIII). > > Hope this helps. For older books, look in AbeBooks, Amazon etc for ones by > GG Brown, HC Cook, CFA Culling, RAB Drury, M Gabe, P Gray, RW Horobin, G > Humason, JFA McManus, AGE Pearse. > > John A. Kiernan > Dept of Anatomy & Cell Biology > University of Western Ontario > London, Canada > = = = > ________________________________ > From: Mac Donald, Jennifer via Histonet > > Sent: May 3, 2021 1:07 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Animal Histology > > > Can anyone recommend a good book for processing animal tissue? > Thanks, > Jennifer > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 612-626-1930 From jmacdonald at mtsac.edu Mon May 3 12:37:14 2021 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Mon, 3 May 2021 17:37:14 +0000 Subject: [Histonet] Animal Histology In-Reply-To: References: Message-ID: Thank you John. From: John Kiernan Sent: Monday, May 3, 2021 10:32 AM To: histonet at lists.utsouthwestern.edu; Mac Donald, Jennifer Subject: Re: Animal Histology EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Jennifer MacDonald asked, "Can anyone recommend a good book for processing animal tissue?" Here's a list of 14 of the ones published since 1990, with very brief descriptive notes. There are, of course, plenty of good books that are older than these. Lyon,H (1991): Theory and Strategy in Histochemistry. A Guide to the Selection and Understanding of Techniques. Springer-Verlag, Berlin. All on theory; no instructions. All aspects of preparation, staining etc are covered. 32 chapters, by different people; one bibliography. Reprinted 2011. ISBN 9783642737442. Kok,LP; Boon,ME (1992): Microwave Cookbook for Microscopists. Art and Science of Visualization. 3rd ed. Coulomb Press Leyden, Leiden. 432 pages. Plenty of microwave theory. Applications in fixation, infiltr, embedding, decalcif., many staining methods including conn.tiss., cytol., neuro. Enzyme, immuno- & hybridization histochemistry. Sanderson,JB (1994): Biological Microtechnique. (Microscopy Handbooks, 28.) BIOS Scientific Publications & Royal Microscopical Society, Oxford. 224 pages. Detailed paractical instructions, with rationale, & titled bibliography for each of the 7 chapters. Collection, Fixation, Processing, Microtomy, Other prep. methods, Staining, Finishing (mounting etc). Chayen,J; Bitensky,L (1991): Practical Histochemistry. 2nd ed. Wiley, Chichester. 321 pages. Special emphasis on use of unfixed cryostat sections. Presnell,JK; Schreibman,MP (1997): Humason's Animal Tissue Techniques. 5th ed. Johns Hopkins University Press, Baltimore. 572 pages. Humason's Animal Tissue Techniques, 5th edition. Includes chapters on immunohistochem, safety, microwaves, lab hints, suppliers, etc. Hayat,MA (1993): Stains and Cytochemical Methods. Plenum Press, New York. Book of techniques. Many are critically discussed. emphasis on EM, but plenty of LM too. Carson,FL; Hladik,C (2009): Histotechnology. A Self-Instructional Text. 3rd ed. American Society of Clinical Pathologists, Chicago. 400 pages. Freida Carson's textbook. 3rd ed, with Hladik, is 96 pages longer than 2nd (1997); with EM and cytopreparation; glossary. ISBN 9780891895817. (There is a 4th ed, 2015, which I haven't seen.) Kiernan,JA (2015): Histological and Histochemical Methods: Theory and Practice. 5th ed. Scion, Banbury, UK. 606 pages. Kumar,GL; Kiernan,JA (Eds.) (2010): Education Guide: Special Stains and H & E. 2nd ed. Dako North America, Carpinteria, CA. 300 pages. 33 chapters. Photos. PDF file is a free download from https://www.agilent.com/en/dako-pathology-education-guides/. Wick,MR (Ed.) (2008): Diagnostic Histochemistry. Cambridge University Press, New York. 460 pages. Multi-author book. Applications of techniques to path. diagnosis. Chapters by systems, organs, diseases etc. Emphasizes value, economy of pre-1970s (before immuno) histochemical methods. Only Ch1, pp.1-27 describes techniques. Many colour photos. Orchard,G; Nation,B (Eds.) (2012): Histopathology. Oxford University Press, Oxford, UK. 396 pages. Multi-author textbook book. Applications of techniques to path. diagnosis. Chapters mainly by methods, with applications to systems, organs, diseases. One of an Inst. of Biomed. Science series, with web site at www.oxfordtextbooks.co.uk/orc/fbs/. Mulisch,M; Welsch,U (Eds.) (2015): Romeis - Mikroskopische Technik. 19th ed. Springer-Verlag, Berlin. 603 pages. 25 chapters, 3 appendices. All types of microscopy, staining, histochem, etc. (in German) ISBN 9783642551901. Exbrayat,JM (Ed.) (2013): Histochemical and Cytochemical Nethods of Visualization. (Series Ed: Morel,G. Methods in Visualization.) CRC Press, Boca Raton, FL. 335 pages. 13 chapters, 10 contributors. 1-7 are for LM; 8-12 for EM; 13 on image quantification. ISBN 9781439822227. Suvarna,SK; Layton,C; Bancroft,JD (Eds.) (2018): Bancroft's Theory and Practice of Histological Techniques. 8th ed. Churchill Livingstone Elsevier, London. 672 pages. In this 8th ed., histochem chapters for lipids, proteins, nucleic acids, enzyme activities are now compressed into Appx I (of VIII). Hope this helps. For older books, look in AbeBooks, Amazon etc for ones by GG Brown, HC Cook, CFA Culling, RAB Drury, M Gabe, P Gray, RW Horobin, G Humason, JFA McManus, AGE Pearse. John A. Kiernan Dept of Anatomy & Cell Biology University of Western Ontario London, Canada = = = ________________________________ From: Mac Donald, Jennifer via Histonet > Sent: May 3, 2021 1:07 AM To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Animal Histology Can anyone recommend a good book for processing animal tissue? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brettmc31 at comcast.net Mon May 3 13:22:21 2021 From: brettmc31 at comcast.net (Brett Connolly) Date: Mon, 3 May 2021 14:22:21 -0400 Subject: [Histonet] Animal Histology In-Reply-To: References: Message-ID: Jennifer, You might want to get the VIR Animal Processing manual from the NSH. Hopefully is it still available?see the bottom of this link. http://nsh.org/sites/default/files/EducationalMaterialsOrder2.7.08-2.pdf Brett Connolly, HTL, PhD (happily retired) Sent from Mail for Windows 10 From: Mac Donald, Jennifer via Histonet Sent: Monday, May 3, 2021 1:08 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Animal Histology Can anyone recommend a good book for processing animal tissue? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Richard.Cartun at hhchealth.org Mon May 3 15:00:05 2021 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 3 May 2021 20:00:05 +0000 Subject: [Histonet] Question - Bone marrow core biopy specimens Message-ID: <383236eef27947209b341c4b0628bb24@hhchealth.org> Hello everyone: I'm wonder what your protocol is for cutting formalin-fixed, paraffin-embedded bone marrow core biopsy specimens? Do you cut a limited number of slides upfront and then go back to the block if the pathologist requests histochemical stains or immunohistochemical tests? We are implementing a protocol today where we will cut "15" slides upfront; 3 H&Es at different levels and 12 unstains for potential histochemical stains and/or IHC tests. Are we crazy? Thank you, and I hope everyone is doing well. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From bakevictoria at gmail.com Mon May 3 15:18:15 2021 From: bakevictoria at gmail.com (Victoria Baker) Date: Mon, 3 May 2021 16:18:15 -0400 Subject: [Histonet] Question - Bone marrow core biopy specimens In-Reply-To: <383236eef27947209b341c4b0628bb24@hhchealth.org> References: <383236eef27947209b341c4b0628bb24@hhchealth.org> Message-ID: Hi Richard! That is the duplicate of what we do in our lab. We were putting both the core and the clot on the same slide for in house testing, but den outs we sometimes have to split the specimen and only on a slide. I have a question for you about ISH Kappa/Lambda. What decal protocol do you use and do you see edge non specific detection/substrate on your slides? We use the Roche Iview blue. I hope what I said helps. Vikki On Mon, May 3, 2021 at 4:12 PM Cartun, Richard via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello everyone: > > I'm wonder what your protocol is for cutting formalin-fixed, > paraffin-embedded bone marrow core biopsy specimens? Do you cut a limited > number of slides upfront and then go back to the block if the pathologist > requests histochemical stains or immunohistochemical tests? We are > implementing a protocol today where we will cut "15" slides upfront; 3 H&Es > at different levels and 12 unstains for potential histochemical stains > and/or IHC tests. Are we crazy? > > Thank you, and I hope everyone is doing well. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic > Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 (Office) > (860) 545-2204 (Fax) > Richard.cartun at hhchealth.org > > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From BMolinari at texasheart.org Wed May 5 08:46:45 2021 From: BMolinari at texasheart.org (Betsy Molinari) Date: Wed, 5 May 2021 13:46:45 +0000 Subject: [Histonet] Movats References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.512dadd3-f848-4ad4-94ad-ce6e3fde3cbe@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.0ddbd0b0-2d28-4166-96a0-c6a77b46dff6@emailsignatures365.codetwo.com> Message-ID: Hi Histonetters, I have received several human vessels for paraffin processing and to stain the sections for H&E and Movats. The H&E were fine. The human sections turned brownish yellow with the Movats.The control which is canine small intestine was perfect. The protocol is standard Bouins 1hr in 58C waterbath Rinse till yellow disappears Rinse in DH2O 1% Alcian Blue -20 min Rinse in running tap H2O -5min Alkaline alcohol-1hr Rinse 10 min tap H2O Rinse in DH2O Verhoff's Hematoxylin -15 min 3 changes DH2O Differentiate in 2% FeCl Rinse in DH2O 5% sodium Thiosulfate -1min Rinse in running tap-10 min Rinse in DH2O Woodstain scarlet/acid fuchsin-1.5 min Rinse in DH2O Rinse in 0.5% acetic acid water 5% aqueous phosphotungstic acid -2 changes 5 min each Rinse in 5% acetic acid water Rinse in 3 changes absolute ETOH 6% alcoholic Safran solution Absolute alcohol-xylene-coverslip The human slides were fine until the Safran step. When I removed them from the stain into the 100% they were a yellowish brown .Under the scope the colors were there, blue, red, yellow and black. But on the slide the tissue was that brownish yellow. The researcher does not like to strong yellow color. Since my control was fine I question if something was going on with their tissue. I do not know how the tissue was handled before it came into the lab. They were very calcified and were decaled for 1-3 days in Cal Rite. I do know they were not rinsed after decal and were put straight back into 10% NBF before I got them for processing. Should I have used a human control instead of canine? These were very large pieces that were crammed into the cassette. Thanks for the help. Sorry for the long post. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 1101 Bates St. Houston,TX 832-355=6524 (lab) 832-355-6812 (fax) Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | texasheartmedical.org | facebook | twitter CONFIDENTIALITY NOTICE: This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From relia1 at earthlink.net Wed May 5 11:03:16 2021 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 5 May 2021 12:03:16 -0400 Subject: [Histonet] Summer is almost here!! What are you doing this Summer? Message-ID: <000501d741c8$29d1a9f0$7d74fdd0$@earthlink.net> Hi Histopeeps, Summer is Almost HERE! I can tell the humidity and the afternoon showers have arrived!! #summerinflorida I am looking forward to beating the heat with some amazing beach days!!! So what are you doing for your Summer Vacation? . Going somewhere exciting? . A Staycation? . Visiting Family or Friends? . Attending a Graduation, A Wedding or Family Reunion? Visiting anyplace you might consider relocation to? If so drop me a line and let me know so I can make a note to keep you posted on positions in that area. Even if we have talked recently drop me a line and remind me!! Here is where my most exciting opportunities are located: . California . Florida . Virginia . Texas . North Carolina . Maryland . Pennsylvania All of my clients offer excellent compensation, benefits, relocation assistance and or sign on bonuses up to 15K! All of these jobs are full time & permanent 1st, 2nd and 3rd shift available! NEW POSITIONS ARE COMING IN DAILY!!! So Drop me a line and let me know where you want to go!! Histopeeps, if you or anyone you know is interested in hearing more about any of these opportunities please contact me. Remember if I place someone you refer to me you will earn a referral bonus!!! Is your lab shorthanded? Did you know that if I get the job opening with your help and place someone in the position you will also earn a referral fee? Ask me how!!!! I can be reached anytime on my cell/text at 407-353-5070 or toll free at 866-607-3542 or e-mail me at relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From karen.heckford at commonspirit.org Wed May 5 12:11:56 2021 From: karen.heckford at commonspirit.org (Karen Heckford CA-San Francisco) Date: Wed, 5 May 2021 10:11:56 -0700 Subject: [Histonet] Thin Prep Message-ID: Does anyone have the contact information for a Thin Prep rep in the US. I need to get in touch with them. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. From lmarie08 at uga.edu Wed May 5 13:59:48 2021 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Wed, 5 May 2021 18:59:48 +0000 Subject: [Histonet] GSH Virtual Summit May 22 Message-ID: Mark Your Calendars and Join Us!! The Georgia Society for Histotechnology invites everyone to our Virtual Summit on May 22, 2021 beginning at 8:00 am until 4:30 pm. This virtual meeting will be a great opportunity to get 6 CEU?s through some excellent speakers/sessions and we hope to do another in fall in order to stay committed to providing the needed education of histotechs. Please see the attached program with the topics of the sessions and visit our website athttps://georgiahistotech.org/gsh-spring-symposium/ for the registration form. Hope to see you there, Ely Klar From lmarie08 at uga.edu Wed May 5 14:29:23 2021 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Wed, 5 May 2021 19:29:23 +0000 Subject: [Histonet] Fw: GSH Virtual Symposium May 22! In-Reply-To: References: Message-ID: Hi All, Here is the clickable link to register: https://georgiahistotech.org/gsh-spring-symposium/ ________________________________ From: Lauren Sweeney Sent: Wednesday, May 5, 2021 2:16 PM To: Histonet at lists.utsouthwestern.edu Subject: GSH Virtual Symposium May 22! Mark Your Calendars and Join Us!! The Georgia Society for Histotechnology invites everyone to our Virtual Summit on May 22, 2021 beginning at 8:00 am until 4:30 pm. This virtual meeting will be a great opportunity to get 6 CEU?s through some excellent speakers/sessions and we hope to do another in fall in order to stay committed to providing the needed education of histotechs. Please see the attached program with the topics of the sessions and visit our website athttps://georgiahistotech.org/gsh-spring-symposium/ for the registration form. Hope to see you there, Ely Klar GSH President From katherine at ka-recruiting.com Thu May 6 08:37:19 2021 From: katherine at ka-recruiting.com (Katherine Marano) Date: Thu, 6 May 2021 09:37:19 -0400 Subject: [Histonet] Histotech opening in NY Message-ID: Hi Histonetters, I'm working with a great lab located on New York's Long Island that is looking to hire a full-time & permanent Histotech ASAP because their volume is growing! They are offering a great pay rate (up to $42/hour) and full benefits. It is a 1st shift position M-F, 5am-1:30pm (the lab isn't even open at nights). Must have experience with processing, embedding, grossing, microtomy, special stains and immunohistochemical stains. I wanted to see if you, or anyone you know, may be interested? Let me know! Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From thisisann at aol.com Thu May 6 09:06:32 2021 From: thisisann at aol.com (Ann Specian) Date: Thu, 6 May 2021 14:06:32 +0000 (UTC) Subject: [Histonet] Xylene Substitute Recycler Validation References: <484006703.1507074.1620309992508.ref@mail.yahoo.com> Message-ID: <484006703.1507074.1620309992508@mail.yahoo.com> We just received a recycler and would like to know if anyone has performed a validation on the recycler? prior to putting into use (separate from the processing validation).? If you have, is it possible to share it with me?? Thank you,? Ann? From jkiernan at uwo.ca Fri May 7 17:11:30 2021 From: jkiernan at uwo.ca (John Kiernan) Date: Fri, 7 May 2021 22:11:30 +0000 Subject: [Histonet] Movats In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.512dadd3-f848-4ad4-94ad-ce6e3fde3cbe@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.0ddbd0b0-2d28-4166-96a0-c6a77b46dff6@emailsignatures365.codetwo.com>, Message-ID: Dear Betsy, Don't say you are sorry for putting a long post on Histonet! To get troubleshooting help you need to say exactly what you did. If you wrote only, "why are my sections brown after Movat staining", nobody would understand your problem. Your procedure starts with an hour in hot Bouin. For many years this has been a routine prior to trichrome stains done on sections of specimens fixed in neutral formaldehyde. It isn't part of Movat's original method (Arch. Path. 60:209-295, 1955), which probably was devised for sections optimally fixed for trichrome staining (in mixtures containing mercuric chloride). Movat's pentachrome is a trichrome method preceded by alcian blue (for no obvious reason) and an iron-haematoxylin for nuclei and elastin. It differs from older trichromes in using a mixture of yellow polyene dyes (saffron) to stain the collagen, instead of the blues or greens as in the Mallory and Masson methods. Your method includes "5% sodium thiosulfate -1 min" after the iron-haematoxylin stain for black nuclei and elastic fibres. This also isn't part of Movat's pentachrome method, and I wonder why. Did you inherit an informal list of instructions passed on within the lab? After a mercuric fixative, hydrated sections are dipped in iodine, followed by thiosulphate, before staining, to remove a black deposit (probably mercurous chloride) introduced by the fixative.I've been seeing similar informal passing of bad staining instructions in research labs for many years. Are you a victim of this trend? The thiosulphate step in your procedure obviously does no harm, because you got the right results with the dog tissues. There may be something different about your human specimens: perhaps inadequate fixation, or excessive acid treatment (if that's what Cal rite is) for decalcification. If the sections of human arteries look OK with a microscope, it might not matter that grossly they are a different colour from the dog small intestine sections. They are, after all, different tissues. A rather long, and not very helpful reply! John Kiernan London, Canada = = = ________________________________ From: Betsy Molinari via Histonet Sent: May 5, 2021 9:46 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Movats Hi Histonetters, I have received several human vessels for paraffin processing and to stain the sections for H&E and Movats. The H&E were fine. The human sections turned brownish yellow with the Movats.The control which is canine small intestine was perfect. The protocol is standard Bouins 1hr in 58C waterbath Rinse till yellow disappears Rinse in DH2O 1% Alcian Blue -20 min Rinse in running tap H2O -5min Alkaline alcohol-1hr Rinse 10 min tap H2O Rinse in DH2O Verhoff's Hematoxylin -15 min 3 changes DH2O Differentiate in 2% FeCl Rinse in DH2O 5% sodium Thiosulfate -1min Rinse in running tap-10 min Rinse in DH2O Woodstain scarlet/acid fuchsin-1.5 min Rinse in DH2O Rinse in 0.5% acetic acid water 5% aqueous phosphotungstic acid -2 changes 5 min each Rinse in 5% acetic acid water Rinse in 3 changes absolute ETOH 6% alcoholic Safran solution Absolute alcohol-xylene-coverslip The human slides were fine until the Safran step. When I removed them from the stain into the 100% they were a yellowish brown .Under the scope the colors were there, blue, red, yellow and black. But on the slide the tissue was that brownish yellow. The researcher does not like to strong yellow color. Since my control was fine I question if something was going on with their tissue. I do not know how the tissue was handled before it came into the lab. They were very calcified and were decaled for 1-3 days in Cal Rite. I do know they were not rinsed after decal and were put straight back into 10% NBF before I got them for processing. Should I have used a human control instead of canine? These were very large pieces that were crammed into the cassette. Thanks for the help. Sorry for the long post. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 1101 Bates St. Houston,TX 832-355=6524 (lab) 832-355-6812 (fax) Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | texasheartmedical.org | facebook | twitter CONFIDENTIALITY NOTICE: This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From 11z at comcast.net Sun May 9 21:29:04 2021 From: 11z at comcast.net (11z at comcast.net) Date: Sun, 9 May 2021 19:29:04 -0700 Subject: [Histonet] doing Sudan Black B Message-ID: <001201d74544$3fa6d5f0$bef481d0$@comcast.net> Hi, I am looking for a protocol for Sudan Black B staining on paraffin embedded tissue. Does anyone have a working stain for this? Thanks LeRoy Brown HT(ASCP) HTL From jessica.vacca at lkmc.com Mon May 10 08:07:06 2021 From: jessica.vacca at lkmc.com (Jessica Vacca) Date: Mon, 10 May 2021 09:07:06 -0400 Subject: [Histonet] JCAHO labs Message-ID: Good Morning! I was wondering if those of you that are accredited by this entity if you wouldn't mind sharing some information. I've only worked in CAP labs and have a complete understanding of the checklists and have based P&P on those. What/Where do the differences lie and in what items should be more focused on. Also if you wouldn't mind sharing some P&P, QC/QA logs etc. that would be great. We have some here but would like to see what others may have consolidated etc. Also we are currently Old School in that we DO NOT have a LIS so everything done here is offline, so in regards to specimen searches, IHC positive stains and Cytology QA is all done manually if you have figured an easy way to do this or can guide me in a process that would work, I'd love to hear your thoughts. I have been on the IT side of things for hte past 12 years and the hospital that I am PRN'ing at needs desperate help in getting some/alot of things done online or availabe on ss that can be easily searched. Equipment/Process: Peloris Ventana ultra Cytology (manual Process) Special Stain (Poly Kits) Leica Stainer Thanks in advance for your help! -- *Jessica Vacca |Histology Technologist | Lower Keys Medical Center | * 5900 College Road I Key West, FL 33040 I | Jessica.Vacca at lkmc.com | Tel: 305-294-5531 x4736 I www.lkmc.com This is a confidential patient safety work product communication. It is protected from disclosure pursuant to the provisions of the Patient Safety and Quality Improvement Act (42 CFR, Part 3) and other state and federal laws. Unauthorized disclosure or duplication is prohibited *Disclaimer*: This electronic message may contain information that is Proprietary, Confidential, or Legally privileged or protected. It is intended only for the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Timothy.Morken at ucsf.edu Mon May 10 11:20:29 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 10 May 2021 16:20:29 +0000 Subject: [Histonet] JCAHO labs In-Reply-To: References: Message-ID: Jessica, CAP and JC apply the same CLIA regulations, just in a different manner. CAP tends to apply more of their own "upgrades." Ie, makes it a bit stricter, which is allowed. CLIA is just the baseline. If you have been thru CAP inspections you will not have any problem with JC. While CAP will campout in your lab for several days and look at EVERYTHING, JC relies on "tracers" to get at the same information, just in a random manner. They will give you several specific dates during the past two years and then ask for a number of random cases per day. Then they want to see everything you have that pertains to those specific cases. That is from accession logs to final report and everything in between - QC logs of stains and equipment, maintenance logs, service reports, controls for whatever stains were done. All slides and blocks on the case. Records of who did the staining. Personnel records of the personnel doing the work, competency records for those personnel. Everything. Like most inspections, if you can produce what they want quickly they are happy. They will also do a walk-through of the lab and ask personnel various questions about safety, where SOPs are, look at chemicals, whether it is too crowded or cluttered, etc. In our labs in 5 JC inspections I have been through here they have never spent more than an hour in any particular lab. But we have many labs, so if they come to inspect your one small lab they will be spending more time there, I imagine. None of the JC inspectors we have had come through had any experience at all in anatomic pathology. All were clinical lab techs or administrators. We got dinged for some things that were minor - chips in formica that a microbiology guy thought were infection hazards, to dust on tops of some cabinets. Not much else. Our JC inspections last 3-4 days but that also covers many hospital areas and other labs besides histo/cyto/grossing/morgue. We have dozens of labs across many facilities. If all they did was AP they would be done in two days at most. IF you have a very small lab it might be only one day. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Jessica Vacca via Histonet Sent: Monday, May 10, 2021 6:07 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] JCAHO labs Good Morning! I was wondering if those of you that are accredited by this entity if you wouldn't mind sharing some information. I've only worked in CAP labs and have a complete understanding of the checklists and have based P&P on those. What/Where do the differences lie and in what items should be more focused on. Also if you wouldn't mind sharing some P&P, QC/QA logs etc. that would be great. We have some here but would like to see what others may have consolidated etc. Also we are currently Old School in that we DO NOT have a LIS so everything done here is offline, so in regards to specimen searches, IHC positive stains and Cytology QA is all done manually if you have figured an easy way to do this or can guide me in a process that would work, I'd love to hear your thoughts. I have been on the IT side of things for hte past 12 years and the hospital that I am PRN'ing at needs desperate help in getting some/alot of things done online or availabe on ss that can be easily searched. Equipment/Process: Peloris Ventana ultra Cytology (manual Process) Special Stain (Poly Kits) Leica Stainer Thanks in advance for your help! -- *Jessica Vacca |Histology Technologist | Lower Keys Medical Center | * 5900 College Road I Key West, FL 33040 I | Jessica.Vacca at lkmc.com | Tel: 305-294-5531 x4736 I https://urldefense.com/v3/__http://www.lkmc.com__;!!LQC6Cpwp!4zmFXwgQHzwbpmPjmD9Vt_u3ESjTyQWRS_M16OLGK30HxLOqahEHjxFJ5h_gRWbxdY057A$ This is a confidential patient safety work product communication. It is protected from disclosure pursuant to the provisions of the Patient Safety and Quality Improvement Act (42 CFR, Part 3) and other state and federal laws. Unauthorized disclosure or duplication is prohibited *Disclaimer*: This electronic message may contain information that is Proprietary, Confidential, or Legally privileged or protected. It is intended only for the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!4zmFXwgQHzwbpmPjmD9Vt_u3ESjTyQWRS_M16OLGK30HxLOqahEHjxFJ5h_gRWaYVh8hCA$ From shultz11 at cox.net Mon May 10 12:09:49 2021 From: shultz11 at cox.net (kendra shultz) Date: Mon, 10 May 2021 12:09:49 -0500 Subject: [Histonet] Job opening Message-ID: <0f43d501-9512-4e48-8bf2-c0c32a250a92@email.android.com> Job opening Research Associate needed for Histology and Immuno at LSU. Apply online at lsu.edu. Thanks, Kendra Shultz 225-578-9724 Kshult1 at lsu.edu From Bonnie.Whitaker at osumc.edu Mon May 10 12:16:01 2021 From: Bonnie.Whitaker at osumc.edu (Whitaker, Bonnie) Date: Mon, 10 May 2021 17:16:01 +0000 Subject: [Histonet] Ohio State University job openings Message-ID: Hi All, We currently have an Evening/Night Supervisor position open, in the OSU Clinical Histology Lab. The hours are generally 4:30 PM-1:00 AM. If you are interested, please email Robyn Mulhall with your resume'. Her email address is Robyn.Mulhall at osumc.edu. Thanks! Bonnie Bonnie Whitaker, MT(HEW), HT(ASCP)QIHC Anatomic Pathology Operations Director The Ohio State University Wexner Medical Center Department of Pathology Suite 6100 Optometry Clinic & Health Sciences Faculty Building 241 11th Avenue Columbus, OH 43210 6 14.293.8418 From POWELL_SA at mercer.edu Tue May 11 09:06:05 2021 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Tue, 11 May 2021 14:06:05 +0000 Subject: [Histonet] GSH update Message-ID: Subject: GSH update The Georgia Society for Histotechnology invites everyone to our Virtual Summit on May 22, 2021 beginning at 8:00 am until 4:30 pm. This virtual meeting will be a great opportunity to get 6 CEU's through some excellent speakers for the 6 sessions. Each session is $10 but if you choose all 6 the cost is $50. We hope to do another in fall in order to stay committed to providing the needed education of histotechs. Please see the program with the topics of the sessions below. Also visit our website at https://georgiahistotech.org/gsh-spring-symposium/ for the program with abstracts, list of vendors and registration form. Hope to see you there, Ely Klar GSH President Georgia Society for Histotechnology GSH 2021 SUMMIT Saturday, May 22, 2021 **VIRTUAL** Itinerary - Saturday, May 22, 2021 8:15am Welcome to the GSH Virtual Summit! - Opening Remarks Ely Klar, MS, HTL(ASCP), Columbus State University, GSH President 8:30am Session I: Exploring Special Stains Carl Sagasser, MBA, BS, HT(ASCP), AmeriPath 9:30am Session II: Her2 Dual ISH: Built For You. Built For Her. Tammy Maury, HTL, Roche/Ventana Pathology Solutions Specialist 10:30am Break I with Virtual Vendor "Exhibits" Various Vendors - See Program for List of Vendors 11:00am Session III: CINtec Plus: The Next Evolution in Cancer Screening Tammy Maury, HTL, Roche/Ventana Pathology Solutions Specialist 12:00pm Session IV: The Veterinary Histology Mystic: Compare and Contrast Shaele Litteral, HT(ASCP), Tifton Veterinary Diagnostic and Investigation Laboratory (TVDIL) 1:00pm Lunch 1:30pm Break II with Virtual Vendor "Exhibits" Various Vendors - See Program for List of Vendors 2:00pm Session V: Performing IHC Procedure Properly Joe Myers, MS, CT(ASCP)QIHC, BioCare Medical LLC 3:00pm Break III with Virtual Vendor "Exhibits" Various Vendors - See Program for List of Vendors 3:30pm Session VI: Troubleshooting Immunohistochemical Staining Procedures Joe Myers, MS, CT(ASCP)QIHC, BioCare Medical LLC 4:30pm Thank you and Closing Remarks Ely Klar, MS, HTL(ASCP), Columbus State University, GSH President *Note: Session access information will be provided to registered participants before the meeting date From relia1 at earthlink.net Tue May 11 11:20:38 2021 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 11 May 2021 12:20:38 -0400 Subject: [Histonet] Histotech needed for day shift position in Maryland can you help? Message-ID: <001701d74681$94e65ed0$beb31c70$@earthlink.net> Hi Histopeeps, How are you? I hope you are having a great week!! I have an exciting opportunity to share and if you aren't interested maybe you know someone who might be! I have been engaged on an exclusive search by one of my best clients located in Annapolis, MD that is in need of a: Full Time Day Shift Histotech!! This is a full time permanent position in a hospital environment. My client is looking for a strong histotech that is ASCP certified HT/HTL, with strong routine histology. CLIA qualified to gross and grossing experience is a plus. They are offering an excellent compensation package. The help I need from you Histopeeps is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them or pass their contact information to me? *remember if I place someone you refer to me you will earn a referral bonus! If you are interested in this position please contact me ASAP on my cell/text 407-353-5070 or toll free at 866-607-3542 or via email at relia1 at earthlink.net I also have exciting opportunities in: . CA . FL . VA . MD . TX . PA . NC . SC If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won't release it to anyone without your permission. Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From 11z at comcast.net Tue May 11 22:05:32 2021 From: 11z at comcast.net (11z at comcast.net) Date: Tue, 11 May 2021 20:05:32 -0700 Subject: [Histonet] microtome part Message-ID: <000001d746db$ac423040$04c690c0$@comcast.net> Anyone happen to have a spare low profile knife holder laying around? Looking for one to fit my MICROM MODEL HM 330. Happy to pay you. It is for my histo tech. She needs a replacement for her microtome. Thanks, LeRoy Brown HT(ASCP) HTL HCS From jkiernan at uwo.ca Wed May 12 10:59:39 2021 From: jkiernan at uwo.ca (John Kiernan) Date: Wed, 12 May 2021 15:59:39 +0000 Subject: [Histonet] doing Sudan Black B In-Reply-To: <001201d74544$3fa6d5f0$bef481d0$@comcast.net> References: <001201d74544$3fa6d5f0$bef481d0$@comcast.net> Message-ID: Hydrated paraffin sections of formaldehyde-fixed tissue are stained the same way as frozen sections of formaldehyde-fixed tissue, but in paraffin sections lipofuscin inclusions are just about the only things that stain. They are, of course, brown or yellow without any staining. If you need to identify lipofuscin inclusions with certainty you'll have to do a few other stains and histochemical tests to distinguish them from ceroid (a similar glycolipoprotein pigment), melanin and haemosiderin. It is not a good idea to use a protocol informally passed on from someone else without checking its origins. Even a method printed in a book can contain errors, and for this reason just about all the methods books published since about 1950 have references to peer-reviewed papers that readers can and sometimes should check. Any book in our field is just a big collection of review articles that have not been reviewed as critically as more specialized reviews in a good journal. The late RD Lillie made important original contributions to pigment histochemistry (two are cited below) and his techniques book (also cited) intelligently reviews the subject and provides technical instructions. So does Pearse's Histochemistry. There are, of course, protocols for staining lipofuscin in all the ordinary histotechniques books. You don't need to have all the latest editions on your lab's bookshelf. The 3rd (1965) edition of Lillie is available for only $8 at https://www.abebooks.co.uk/book-search/title/histopathologic-technic-and-practical-histochemistry/author/r-d-lillie/ Histopathologic Technic and Practical Histochemistry by R D Lillie - AbeBooks Histopathologic Technic and Practical Histochemistry by Lillie, R D and a great selection of related books, art and collectibles available now at AbeBooks.co.uk. www.abebooks.co.uk Snap it up! The 4th and last (1976) edition of Lillie is now $700 from Amazon, but the 1965 edition is still OK for most of what the book is about, and it's easier to read than his rather rambling 4th edition. Lillie RD, Fullmer HM (1976) Histopathologic Technic and Practical Histochemistry. 4th ed. McGraw-Hill, New York. See comments above - the previous edition is cheaper and for you it may be just as good. Pearse AGE, Stoward PJ (1980,1985,1991) Histochemistry, Theoretical and Applied, 4th edn. Vol. 1. Preparative and Optical Technology. Vol. 2. Analytical Technique. Vol. 3. Enzyme Histochemistry. v. 1,2,3. Churchill-Livingstone, Edinburgh. Pigments are in Vol. 2. Third edn (1968. 2 vols) probably just as good for most labs. Lillie RD (1956) The mechanism of Nile blue staining of lipofuscins. J. Histochem. Cytochem. 4:377-381. I think all the papers in this journal are now free for anyone to download. Lillie RD (1956) A Nile blue staining technic for the differentiation of melanin and lipofuscin. Stain Technol. 31:151-153. Sadly, not free unless you have access to a subscribing library or are a member of the Biological Stain Commission. I hope this answer helps. John Kiernan = = = = ________________________________ From: LEROY H BROWN via Histonet Sent: May 9, 2021 10:29 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] doing Sudan Black B Hi, I am looking for a protocol for Sudan Black B staining on paraffin embedded tissue. Does anyone have a working stain for this? Thanks LeRoy Brown HT(ASCP) HTL _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jhill at vet.k-state.edu Thu May 13 08:17:58 2021 From: jhill at vet.k-state.edu (Jennifer Phinney) Date: Thu, 13 May 2021 13:17:58 +0000 Subject: [Histonet] Automated Coverslippers Message-ID: Hello Histonetters, My lab currently has a Leica CV5030 that is attached to our ST5010 Autostainer. We bought it way back in 2012 and it is starting to have progressively more issues working reliably. This is the only one that can be integrated with our stainer, so we know we'll have to get a standalone unit and transfer slides to a new rack. What automated coverslipper is everyone using? I know in the past there have been issues with tape coverslippers popping off of the slides when stored long term, but has this been fixed with newer units? What is everyone's experience with them? I got a quote for the Leica Spectra coverslipper, but I am not familiar with what other companies have available. Thanks all, Jennifer Phinney MS QIHC Histology Laboratory Administrator Kansas State Veterinary Diagnostic Lab From patpxs at gmail.com Thu May 13 09:31:12 2021 From: patpxs at gmail.com (Patpxs) Date: Thu, 13 May 2021 07:31:12 -0700 Subject: [Histonet] Automated Coverslippers In-Reply-To: References: Message-ID: <042A4EFB-5C0E-40DB-A324-B0B438C87E43@gmail.com> Happy Friday Eve, We use the Sakura glas2 cover slipper. We run hundreds of slides through them every day with very few problems. Any automated coverslipper can have issues. A word of warning- watching the g2 do its thing is hypnotic. Paula Sent from my iPhone > On May 13, 2021, at 6:18 AM, Jennifer Phinney via Histonet wrote: > > ?Hello Histonetters, > My lab currently has a Leica CV5030 that is attached to our ST5010 Autostainer. We bought it way back in 2012 and it is starting to have progressively more issues working reliably. This is the only one that can be integrated with our stainer, so we know we'll have to get a standalone unit and transfer slides to a new rack. > > What automated coverslipper is everyone using? I know in the past there have been issues with tape coverslippers popping off of the slides when stored long term, but has this been fixed with newer units? What is everyone's experience with them? > > I got a quote for the Leica Spectra coverslipper, but I am not familiar with what other companies have available. > > Thanks all, > Jennifer Phinney MS QIHC > Histology Laboratory Administrator > Kansas State Veterinary Diagnostic Lab > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari at texasheart.org Thu May 13 09:43:13 2021 From: BMolinari at texasheart.org (Betsy Molinari) Date: Thu, 13 May 2021 14:43:13 +0000 Subject: [Histonet] Movats In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.512dadd3-f848-4ad4-94ad-ce6e3fde3cbe@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.0ddbd0b0-2d28-4166-96a0-c6a77b46dff6@emailsignatures365.codetwo.com>, Message-ID: Hi John, Thank you so much for your reply. This staining protocol is a "hand me down" . I have been using it for years with positive feed back so I had the "if it ain't broke don't fix it" mentality. This changed with this batch of samples. I just finished the study with 40 Movats total. The controls with each batch were fine and all the tissue from the client stained consistently with the strong yellow staining. They are satisfied with the results and we have discussed how to treat future samples. I did run controls skipping the Bouins step and the sodium thiosulfate . There was no discernable difference , so I saved at least an hour. I will miss the aroma of hot Bouins (NOT!) . I have always learned something new or thought provoking with your posts. Thank you! Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | texasheartmedical.org | facebook | twitter From: John Kiernan Sent: Friday, May 7, 2021 5:12 PM To: histonet at lists.utsouthwestern.edu; Betsy Molinari Subject: Re: Movats *** Important *** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Dear Betsy, Don't say you are sorry for putting a long post on Histonet! To get troubleshooting help you need to say exactly what you did. If you wrote only, "why are my sections brown after Movat staining", nobody would understand your problem. Your procedure starts with an hour in hot Bouin. For many years this has been a routine prior to trichrome stains done on sections of specimens fixed in neutral formaldehyde. It isn't part of Movat's original method (Arch. Path. 60:209-295, 1955), which probably was devised for sections optimally fixed for trichrome staining (in mixtures containing mercuric chloride). Movat's pentachrome is a trichrome method preceded by alcian blue (for no obvious reason) and an iron-haematoxylin for nuclei and elastin. It differs from older trichromes in using a mixture of yellow polyene dyes (saffron) to stain the collagen, instead of the blues or greens as in the Mallory and Masson methods. Your method includes "5% sodium thiosulfate -1 min" after the iron-haematoxylin stain for black nuclei and elastic fibres. This also isn't part of Movat's pentachrome method, and I wonder why. Did you inherit an informal list of instructions passed on within the lab? After a mercuric fixative, hydrated sections are dipped in iodine, followed by thiosulphate, before staining, to remove a black deposit (probably mercurous chloride) introduced by the fixative.I've been seeing similar informal passing of bad staining instructions in research labs for many years. Are you a victim of this trend? The thiosulphate step in your procedure obviously does no harm, because you got the right results with the dog tissues. There may be something different about your human specimens: perhaps inadequate fixation, or excessive acid treatment (if that's what Cal rite is) for decalcification. If the sections of human arteries look OK with a microscope, it might not matter that grossly they are a different colour from the dog small intestine sections. They are, after all, different tissues. A rather long, and not very helpful reply! John Kiernan London, Canada = = = ________________________________ From: Betsy Molinari via Histonet > Sent: May 5, 2021 9:46 AM To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Movats Hi Histonetters, I have received several human vessels for paraffin processing and to stain the sections for H&E and Movats. The H&E were fine. The human sections turned brownish yellow with the Movats.The control which is canine small intestine was perfect. The protocol is standard Bouins 1hr in 58C waterbath Rinse till yellow disappears Rinse in DH2O 1% Alcian Blue -20 min Rinse in running tap H2O -5min Alkaline alcohol-1hr Rinse 10 min tap H2O Rinse in DH2O Verhoff's Hematoxylin -15 min 3 changes DH2O Differentiate in 2% FeCl Rinse in DH2O 5% sodium Thiosulfate -1min Rinse in running tap-10 min Rinse in DH2O Woodstain scarlet/acid fuchsin-1.5 min Rinse in DH2O Rinse in 0.5% acetic acid water 5% aqueous phosphotungstic acid -2 changes 5 min each Rinse in 5% acetic acid water Rinse in 3 changes absolute ETOH 6% alcoholic Safran solution Absolute alcohol-xylene-coverslip The human slides were fine until the Safran step. When I removed them from the stain into the 100% they were a yellowish brown .Under the scope the colors were there, blue, red, yellow and black. But on the slide the tissue was that brownish yellow. The researcher does not like to strong yellow color. Since my control was fine I question if something was going on with their tissue. I do not know how the tissue was handled before it came into the lab. They were very calcified and were decaled for 1-3 days in Cal Rite. I do know they were not rinsed after decal and were put straight back into 10% NBF before I got them for processing. Should I have used a human control instead of canine? These were very large pieces that were crammed into the cassette. Thanks for the help. Sorry for the long post. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 1101 Bates St. Houston,TX 832-355=6524 (lab) 832-355-6812 (fax) Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | texasheartmedical.org> | twitter> CONFIDENTIALITY NOTICE: This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From john.garratt at ciqc.ca Thu May 13 10:47:41 2021 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 13 May 2021 15:47:41 +0000 Subject: [Histonet] Automated Coverslippers In-Reply-To: <042A4EFB-5C0E-40DB-A324-B0B438C87E43@gmail.com> References: <042A4EFB-5C0E-40DB-A324-B0B438C87E43@gmail.com> Message-ID: <6iUk9Ms516R2AJdL2i8oSXd356BdELextV-LdI0o103lSX3CQk-AGv-oFR4dG-KTEzLDQXnY3_4jE1sV7-rGFkQIi2QcusaZHF4crn8smJI=@ciqc.ca> Having used tape coverslips for over 20 years previous to moving out of the clinical lab I can say that I have rarely had a problem with them. They are efficient because they dry so quickly so they can be filed efficiently. Important for a Lean operation. The key to success with tape coverslips is to use good quality xylene ( not recycled ) and make sure you use enough xylene (do not skimp). John. On Thu, May 13, 2021 at 7:31 AM, Patpxs via Histonet wrote: > Happy Friday Eve, > > We use the Sakura glas2 cover slipper. We run hundreds of slides through them every day with very few problems. > > Any automated coverslipper can have issues. A word of warning- watching the g2 do its thing is hypnotic. > > Paula > > Sent from my iPhone > >> On May 13, 2021, at 6:18 AM, Jennifer Phinney via Histonet wrote: >> >> ?Hello Histonetters, >> My lab currently has a Leica CV5030 that is attached to our ST5010 Autostainer. We bought it way back in 2012 and it is starting to have progressively more issues working reliably. This is the only one that can be integrated with our stainer, so we know we'll have to get a standalone unit and transfer slides to a new rack. >> >> What automated coverslipper is everyone using? I know in the past there have been issues with tape coverslippers popping off of the slides when stored long term, but has this been fixed with newer units? What is everyone's experience with them? >> >> I got a quote for the Leica Spectra coverslipper, but I am not familiar with what other companies have available. >> >> Thanks all, >> Jennifer Phinney MS QIHC >> Histology Laboratory Administrator >> Kansas State Veterinary Diagnostic Lab >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Thu May 13 11:17:13 2021 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 13 May 2021 12:17:13 -0400 Subject: [Histonet] Tomorrow is the LAST Day for the ASCP 2021 Wage Survey. Can you help? Message-ID: <007a01d74813$701a28a0$504e79e0$@earthlink.net> Hi Histopeeps! I am passing this important information along from NSH: Histopeeps!!! Tomorrow is the final day to participate in the ASCP 2021 Wage and Vacancy Survey. These surveys are a primary source for salary information and will aid you in your job searches and sourcing candidates. We prohibit salary discussion but will refer group participants to the surveys. The more data we collect on histotechnicians and histotechnologists, the more valuable the report will be. The Survey will take approximately 20 minutes to complete. Please share this link with your histotech colleagues. Take the Survey Now! https://app.keysurvey.com/f/41561267/15af/ Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From Timothy.Morken at ucsf.edu Thu May 13 11:59:37 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 13 May 2021 16:59:37 +0000 Subject: [Histonet] Automated Coverslippers In-Reply-To: References: Message-ID: Jennifer, we have used the Sakura Glas, Glas2 (attached to prisma stainer), and now the Sakura tape cover slipper attached to the Prisma. Also we have had the Leica CV5030 glass coverslipper. All worked as long as they are kept clean. Daily maintenance is a must. If in a service contract they can last until parts are no longer available. However, we moved to tape once we started scanning all our slides. Tape coverslipping is much faster and it dries instantly, allowing scanning immediately. The pop-off issues with the tape was solved 20 years ago, so it has not been a problem. The only problem we have seen is that the tape does get scratched if the slides are handled a lot outside of flats. Previous to scanning we pulled slides a lot for conferences so pathologists did not like it. Once we started scanning that became a moot issue - no one pulls slides anymore except for the rare send out consultation. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Jennifer Phinney via Histonet Sent: Thursday, May 13, 2021 6:18 AM To: HistoNet Subject: [Histonet] Automated Coverslippers Hello Histonetters, My lab currently has a Leica CV5030 that is attached to our ST5010 Autostainer. We bought it way back in 2012 and it is starting to have progressively more issues working reliably. This is the only one that can be integrated with our stainer, so we know we'll have to get a standalone unit and transfer slides to a new rack. What automated coverslipper is everyone using? I know in the past there have been issues with tape coverslippers popping off of the slides when stored long term, but has this been fixed with newer units? What is everyone's experience with them? I got a quote for the Leica Spectra coverslipper, but I am not familiar with what other companies have available. Thanks all, Jennifer Phinney MS QIHC Histology Laboratory Administrator Kansas State Veterinary Diagnostic Lab _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!70_GFpM7UgNBjvPgIE62P12XHESQtfDlKuGGOHe6yJF-mGRuEeRfXTyCr0yNyTybinplAA$ From histogirl57 at gmail.com Mon May 17 13:11:09 2021 From: histogirl57 at gmail.com (Histo Girl) Date: Mon, 17 May 2021 14:11:09 -0400 Subject: [Histonet] Controls Message-ID: Hi fellow Histo people! I am setting up a new histology lab and I am needing to find inventive ways to obtain controls for IHC and Special stains. I have lots of normal tissue but I am in need of melanoma, P16 positive, fungus, positive p63, and positive p504. If anyone would be interested in helping me out I would greatly appreciate it. Thanks! From rmgriffey at ucdavis.edu Mon May 17 16:25:50 2021 From: rmgriffey at ucdavis.edu (Rebecca M Griffey) Date: Mon, 17 May 2021 21:25:50 +0000 Subject: [Histonet] University of California job post: LAB RSCH SUPV 1 (Histology Lab Supervisor) Message-ID: Anyone interested in a full-time Histology lab Supervisor position at UC Davis in the School of Veterinary Medicine. Follow the link and/or contact me with questions. Position: LAB RSCH SUPV 1 (Histology Lab Supervisor) Location: Davis Type: Full Time Salary: $54400 - $109200 Category: Research and Laboratory Requisition: 17863 Posting Date: 4/27/2021 More Information: https://careerspub.universityofcalifornia.edu/psp/ucdavis/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_APP_SCHJOB.GBL?Page=HRS_APP_JBPST&JobOpeningId=17863&PostingSeq=1&SiteId=7&languageCd=ENG&FOCUS=Applicant Rebecca M. Griffey Anatomic Pathology Service Manager William R. Pritchard Veterinary Medical Teaching Hospital 1345 VM3A; 1 Shields Ave. University of California, Davis Davis, CA 95616 Voice: 530-752-1369 Fax: 530-754-1400 rmgriffey at ucdavis.edu #1 Ranked Veterinary School in the Nation Residency Training in Anatomic Pathology of UC Davis VMTH Anatomic Pathology Service From bhethcox at hcmhcares.org Wed May 19 09:21:02 2021 From: bhethcox at hcmhcares.org (Brittany Hethcox) Date: Wed, 19 May 2021 10:21:02 -0400 Subject: [Histonet] Cryostat Message-ID: Hello everyone! I am new to the Histology world. You all may know my previous coworker, Rhonda Ford. She officially retired and I am so happy for her! With that being said, I am in a pickle because the cryostat we had completely broke down. We were having issues with it during my training period, so I never officially got to use it. We are now looking for a new cryostat for our department. My supervisor is asking me about different models and features, but I don't know much about any of this. So I was hoping you all could help! Here are the details: We are looking at two different models: - HM525NX - CRYOSTAR NX50 We are also getting questions about UV versus fog mist (More specifically the "Cold D" fog mist). So my questions are: 1) Which model do you think is a better investment? 2) Is it ok to buy used? - It's about half the price, which my supervisor really appreciates! - A new cryostat will have to come from overseas. The seller says used ones will probably be available in the states. 3) Which is better, UV or fog mist? 4) Are there any other features that we should aim to include in our purchase (e.g. height adjustment, vacutome, etc.)? Thanks so much for your help! -- Brittany Hethcox Histology/Pathology 765-521-1284 Henry Community Health -- *"We Make Lasting Connections"*Henry Community Health From Timothy.Morken at ucsf.edu Wed May 19 10:26:13 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 19 May 2021 15:26:13 +0000 Subject: [Histonet] Cryostat In-Reply-To: References: Message-ID: Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're reliable and easy to use. We have the elevator on ours and it makes a difference for different size people in the lab. It also can have a vapor disinfectant system which we use and it works great. We use ours for kidney and muscle cryosectioning, not gross room/OR sections but these would work fine for that. They have a lot of room inside for specimens. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Brittany Hethcox via Histonet Sent: Wednesday, May 19, 2021 7:21 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cryostat Hello everyone! I am new to the Histology world. You all may know my previous coworker, Rhonda Ford. She officially retired and I am so happy for her! With that being said, I am in a pickle because the cryostat we had completely broke down. We were having issues with it during my training period, so I never officially got to use it. We are now looking for a new cryostat for our department. My supervisor is asking me about different models and features, but I don't know much about any of this. So I was hoping you all could help! Here are the details: We are looking at two different models: - HM525NX - CRYOSTAR NX50 We are also getting questions about UV versus fog mist (More specifically the "Cold D" fog mist). So my questions are: 1) Which model do you think is a better investment? 2) Is it ok to buy used? - It's about half the price, which my supervisor really appreciates! - A new cryostat will have to come from overseas. The seller says used ones will probably be available in the states. 3) Which is better, UV or fog mist? 4) Are there any other features that we should aim to include in our purchase (e.g. height adjustment, vacutome, etc.)? Thanks so much for your help! -- Brittany Hethcox Histology/Pathology 765-521-1284 Henry Community Health -- *"We Make Lasting Connections"*Henry Community Health _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!74SoiCnESMaSi9f-mZZu3TuQpthIBblOoUALh7lOXnMsH4PDJqAkjJJaOlLf5219UuzvTw$ From Timothy.Morken at ucsf.edu Wed May 19 10:41:31 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 19 May 2021 15:41:31 +0000 Subject: [Histonet] Cryostat In-Reply-To: References: Message-ID: Guess I missed your other questions. I think the fog mist (Cold D) may be better - it reaches all area of the chamber. UV light is shaded from some areas, though probably hits most areas you might be touching. The misting requires a specific solution, not just anything. Hydrogen peroxide is the disinfecting agent. Other features: We have the vacutome on the original units but we never use it. We do all kidney and muscle sectioning and these small samples do not produce much debris. On our new replacement unit (to replace a 7-year old NX70) we opted out of the vacuum. If using in a gross room/Or setting the vacuum may be very useful. It only adds a hose and suction manifold in the unit and does not get in the way of anything. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Morken, Timothy via Histonet Sent: Wednesday, May 19, 2021 8:26 AM To: Brittany Hethcox Cc: Histonet Subject: Re: [Histonet] Cryostat Brittany, we have two of the Epredia Cryostar NX70 and love them. They 're reliable and easy to use. We have the elevator on ours and it makes a difference for different size people in the lab. It also can have a vapor disinfectant system which we use and it works great. We use ours for kidney and muscle cryosectioning, not gross room/OR sections but these would work fine for that. They have a lot of room inside for specimens. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Brittany Hethcox via Histonet Sent: Wednesday, May 19, 2021 7:21 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cryostat Hello everyone! I am new to the Histology world. You all may know my previous coworker, Rhonda Ford. She officially retired and I am so happy for her! With that being said, I am in a pickle because the cryostat we had completely broke down. We were having issues with it during my training period, so I never officially got to use it. We are now looking for a new cryostat for our department. My supervisor is asking me about different models and features, but I don't know much about any of this. So I was hoping you all could help! Here are the details: We are looking at two different models: - HM525NX - CRYOSTAR NX50 We are also getting questions about UV versus fog mist (More specifically the "Cold D" fog mist). So my questions are: 1) Which model do you think is a better investment? 2) Is it ok to buy used? - It's about half the price, which my supervisor really appreciates! - A new cryostat will have to come from overseas. The seller says used ones will probably be available in the states. 3) Which is better, UV or fog mist? 4) Are there any other features that we should aim to include in our purchase (e.g. height adjustment, vacutome, etc.)? Thanks so much for your help! -- Brittany Hethcox Histology/Pathology 765-521-1284 Henry Community Health -- *"We Make Lasting Connections"*Henry Community Health _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!74SoiCnESMaSi9f-mZZu3TuQpthIBblOoUALh7lOXnMsH4PDJqAkjJJaOlLf5219UuzvTw$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!_ds9AtFEC5PZbUsRGfDv3ncG8lFW-7mf6aFJqWETo4wBI602GQBADwNr2eFqLbYJXlGeDw$ From TNMayer at mdanderson.org Wed May 19 13:04:16 2021 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Wed, 19 May 2021 18:04:16 +0000 Subject: [Histonet] Movats Message-ID: <265b9b0dd7a04f8e8b16a32f074df477@mdanderson.org> Hey Betsy, It could also be the woodstain scarlet and the saffron. I would omit the 5% acetic after the phosphotungstic. Take care, Toysha Mayer Message: 1 Date: Fri, 7 May 2021 22:11:30 +0000 From: John Kiernan To: "histonet at lists.utsouthwestern.edu" , Betsy Molinari Subject: Re: [Histonet] Movats Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dear Betsy, Don't say you are sorry for putting a long post on Histonet! To get troubleshooting help you need to say exactly what you did. If you wrote only, "why are my sections brown after Movat staining", nobody would understand your problem. Your procedure starts with an hour in hot Bouin. For many years this has been a routine prior to trichrome stains done on sections of specimens fixed in neutral formaldehyde. It isn't part of Movat's original method (Arch. Path. 60:209-295, 1955), which probably was devised for sections optimally fixed for trichrome staining (in mixtures containing mercuric chloride). Movat's pentachrome is a trichrome method preceded by alcian blue (for no obvious reason) and an iron-haematoxylin for nuclei and elastin. It differs from older trichromes in using a mixture of yellow polyene dyes (saffron) to stain the collagen, instead of the blues or greens as in the Mallory and Masson methods. Your method includes "5% sodium thiosulfate -1 min" after the iron-haematoxylin stain for black nuclei and elastic fibres. This also isn't part of Movat's pentachrome method, and I wonder why. Did you inherit an informal list of instructions passed on within the lab? After a mercuric fixative, hydrated sections are dipped in iodine, followed by thiosulphate, before staining, to remove a black deposit (probably mercurous chloride) introduced by the fixative.I've been seeing similar informal passing of bad staining instructions in research labs for many years. Are you a victim of this trend? The thiosulphate step in your procedure obviously does no harm, because you got the right results with the dog tissues. There may be something different about your human specimens: perhaps inadequate fixation, or excessive acid treatment (if that's what Cal rite is) for decalcification. If the sections of human arteries look OK with a microscope, it might not matter that grossly they are a different colour from the dog small intestine sections. They are, after all, different tissues. A rather long, and not very helpful reply! John Kiernan London, Canada = = = ________________________________ From: Betsy Molinari via Histonet Sent: May 5, 2021 9:46 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Movats Hi Histonetters, I have received several human vessels for paraffin processing and to stain the sections for H&E and Movats. The H&E were fine. The human sections turned brownish yellow with the Movats.The control which is canine small intestine was perfect. The protocol is standard Bouins 1hr in 58C waterbath Rinse till yellow disappears Rinse in DH2O 1% Alcian Blue -20 min Rinse in running tap H2O -5min Alkaline alcohol-1hr Rinse 10 min tap H2O Rinse in DH2O Verhoff's Hematoxylin -15 min 3 changes DH2O Differentiate in 2% FeCl Rinse in DH2O 5% sodium Thiosulfate -1min Rinse in running tap-10 min Rinse in DH2O Woodstain scarlet/acid fuchsin-1.5 min Rinse in DH2O Rinse in 0.5% acetic acid water 5% aqueous phosphotungstic acid -2 changes 5 min each Rinse in 5% acetic acid water Rinse in 3 changes absolute ETOH 6% alcoholic Safran solution Absolute alcohol-xylene-coverslip The human slides were fine until the Safran step. When I removed them from the stain into the 100% they were a yellowish brown .Under the scope the colors were there, blue, red, yellow and black. But on the slide the tissue was that brownish yellow. The researcher does not like to strong yellow color. Since my control was fine I question if something was going on with their tissue. I do not know how the tissue was handled before it came into the lab. They were very calcified and were decaled for 1-3 days in Cal Rite. I do know they were not rinsed after decal and were put straight back into 10% NBF before I got them for processing. Should I have used a human control instead of canine? These were very large pieces that were crammed into the cassette. Thanks for the help. Sorry for the long post. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 1101 Bates St. Houston,TX 832-355=6524 (lab) 832-355-6812 (fax) Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | texasheartmedical.org | facebook | twitter CONFIDENTIALITY NOTICE: This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PfbeBCCAmug!wzfab7qYW4xTqIVaO5gZ8hZnKbB0B7NR6-rNUpGwMSKTNuo2Q6VczFcFIeDklobUHg$ ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PfbeBCCAmug!wzfab7qYW4xTqIVaO5gZ8hZnKbB0B7NR6-rNUpGwMSKTNuo2Q6VczFcFIeDklobUHg$ ------------------------------ End of Histonet Digest, Vol 210, Issue 6 **************************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From lmarie08 at uga.edu Wed May 19 15:24:02 2021 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Wed, 19 May 2021 20:24:02 +0000 Subject: [Histonet] GSH Virtual Summit- THIS SATURDAY Message-ID: Hi Everyone, It is not too late to join us for the GSH Virtual Summit on May 22, 2021 beginning at 8:00 am until 4:30 pm. This virtual meeting will be a great opportunity to get 6 CEU?s through some excellent speakers/sessions and we hope to do another in the fall in order to stay committed to providing the needed education of histotechs. Please visit our website at https://georgiahistotech.org/gsh-spring-symposium/ for the registration form. Cost is $10.00 per session or $50.00 for all 6 sessions. You can do 1, 2 or more sessions for the day. Hope to see you there, Ely Klar GSH President From cforster at umn.edu Wed May 19 15:38:13 2021 From: cforster at umn.edu (Colleen Forster) Date: Wed, 19 May 2021 15:38:13 -0500 Subject: [Histonet] GSH Virtual Summit- THIS SATURDAY In-Reply-To: References: Message-ID: I'd love to join you but I will be working that entire day.... Colleen Forster On Wed, May 19, 2021 at 3:24 PM Lauren Sweeney via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Everyone, > > It is not too late to join us for the GSH Virtual Summit on May 22, 2021 > beginning at 8:00 am until 4:30 pm. > This virtual meeting will be a great opportunity to get 6 CEU?s through > some excellent speakers/sessions and we hope to do another in the fall in > order to stay committed to providing the needed education of histotechs. > Please visit our website at > https://georgiahistotech.org/gsh-spring-symposium/ for the registration > form. Cost is $10.00 per session or $50.00 for all 6 sessions. You can do > 1, 2 or more sessions for the day. > Hope to see you there, > Ely Klar > GSH President > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 612-626-1930 From penny.marr at nhs.net Thu May 20 03:32:47 2021 From: penny.marr at nhs.net (MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST)) Date: Thu, 20 May 2021 08:32:47 +0000 Subject: [Histonet] Cryostat In-Reply-To: References: Message-ID: Hi Brittany, Personally I do not like the HM525NX. It is awkward and difficult to control with no manual advance except the control panel. When advancing /trimming you are never certain how far forward the block will travel. The UV light fitting in the lid always seems to get in the way of my field of view. Ours doesn't have temperature control of the block holder which can make sectioning more difficult. Looking at the specs of the cryostar I would actually opt for the NX70. Kind regards, Penny :) Penny Marr Senior BMS C/- Histology Conquest Hospital St Leonards-on-Sea TN37 7RD penny.marr at nhs.net 0300 131 4500 ext 734914 0300 131 4914 -----Original Message----- From: Brittany Hethcox [mailto:bhethcox at hcmhcares.org] Sent: 19 May 2021 15:21 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cryostat Hello everyone! I am new to the Histology world. You all may know my previous coworker, Rhonda Ford. She officially retired and I am so happy for her! With that being said, I am in a pickle because the cryostat we had completely broke down. We were having issues with it during my training period, so I never officially got to use it. We are now looking for a new cryostat for our department. My supervisor is asking me about different models and features, but I don't know much about any of this. So I was hoping you all could help! Here are the details: We are looking at two different models: - HM525NX - CRYOSTAR NX50 We are also getting questions about UV versus fog mist (More specifically the "Cold D" fog mist). So my questions are: 1) Which model do you think is a better investment? 2) Is it ok to buy used? - It's about half the price, which my supervisor really appreciates! - A new cryostat will have to come from overseas. The seller says used ones will probably be available in the states. 3) Which is better, UV or fog mist? 4) Are there any other features that we should aim to include in our purchase (e.g. height adjustment, vacutome, etc.)? Thanks so much for your help! -- Brittany Hethcox Histology/Pathology 765-521-1284 Henry Community Health -- *"We Make Lasting Connections"*Henry Community Health ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in relation to its contents. To do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland. NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and other accredited email services. For more information and to find out how you can switch, https://portal.nhs.net/help/joiningnhsmail From BMolinari at texasheart.org Fri May 21 10:01:06 2021 From: BMolinari at texasheart.org (Betsy Molinari) Date: Fri, 21 May 2021 15:01:06 +0000 Subject: [Histonet] Movats In-Reply-To: <265b9b0dd7a04f8e8b16a32f074df477@mdanderson.org> References: <265b9b0dd7a04f8e8b16a32f074df477@mdanderson.org> <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.d67a75db-0fd9-42b4-8bf8-43da1dde7324@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.55951d76-de8d-4dc1-9c13-12e4f09f469e@emailsignatures365.codetwo.com> Message-ID: Thanks for the reply Toysha. That was a typo. I should have typed 0.5%. Thanks again. Hope all is well! Betsy Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | texasheartmedical.org | facebook | twitter -----Original Message----- From: Mayer,Toysha N via Histonet Sent: Wednesday, May 19, 2021 1:04 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Movats *** Important *** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Hey Betsy, It could also be the woodstain scarlet and the saffron. I would omit the 5% acetic after the phosphotungstic. Take care, Toysha Mayer Message: 1 Date: Fri, 7 May 2021 22:11:30 +0000 From: John Kiernan To: "histonet at lists.utsouthwestern.edu" , Betsy Molinari Subject: Re: [Histonet] Movats Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dear Betsy, Don't say you are sorry for putting a long post on Histonet! To get troubleshooting help you need to say exactly what you did. If you wrote only, "why are my sections brown after Movat staining", nobody would understand your problem. Your procedure starts with an hour in hot Bouin. For many years this has been a routine prior to trichrome stains done on sections of specimens fixed in neutral formaldehyde. It isn't part of Movat's original method (Arch. Path. 60:209-295, 1955), which probably was devised for sections optimally fixed for trichrome staining (in mixtures containing mercuric chloride). Movat's pentachrome is a trichrome method preceded by alcian blue (for no obvious reason) and an iron-haematoxylin for nuclei and elastin. It differs from older trichromes in using a mixture of yellow polyene dyes (saffron) to stain the collagen, instead of the blues or greens as in the Mallory and Masson methods. Your method includes "5% sodium thiosulfate -1 min" after the iron-haematoxylin stain for black nuclei and elastic fibres. This also isn't part of Movat's pentachrome method, and I wonder why. Did you inherit an informal list of instructions passed on within the lab? After a mercuric fixative, hydrated sections are dipped in iodine, followed by thiosulphate, before staining, to remove a black deposit (probably mercurous chloride) introduced by the fixative.I've been seeing similar informal passing of bad staining instructions in research labs for many years. Are you a victim of this trend? The thiosulphate step in your procedure obviously does no harm, because you got the right results with the dog tissues. There may be something different about your human specimens: perhaps inadequate fixation, or excessive acid treatment (if that's what Cal rite is) for decalcification. If the sections of human arteries look OK with a microscope, it might not matter that grossly they are a different colour from the dog small intestine sections. They are, after all, different tissues. A rather long, and not very helpful reply! John Kiernan London, Canada = = = ________________________________ From: Betsy Molinari via Histonet Sent: May 5, 2021 9:46 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Movats Hi Histonetters, I have received several human vessels for paraffin processing and to stain the sections for H&E and Movats. The H&E were fine. The human sections turned brownish yellow with the Movats.The control which is canine small intestine was perfect. The protocol is standard Bouins 1hr in 58C waterbath Rinse till yellow disappears Rinse in DH2O 1% Alcian Blue -20 min Rinse in running tap H2O -5min Alkaline alcohol-1hr Rinse 10 min tap H2O Rinse in DH2O Verhoff's Hematoxylin -15 min 3 changes DH2O Differentiate in 2% FeCl Rinse in DH2O 5% sodium Thiosulfate -1min Rinse in running tap-10 min Rinse in DH2O Woodstain scarlet/acid fuchsin-1.5 min Rinse in DH2O Rinse in 0.5% acetic acid water 5% aqueous phosphotungstic acid -2 changes 5 min each Rinse in 5% acetic acid water Rinse in 3 changes absolute ETOH 6% alcoholic Safran solution Absolute alcohol-xylene-coverslip The human slides were fine until the Safran step. When I removed them from the stain into the 100% they were a yellowish brown .Under the scope the colors were there, blue, red, yellow and black. But on the slide the tissue was that brownish yellow. The researcher does not like to strong yellow color. Since my control was fine I question if something was going on with their tissue. I do not know how the tissue was handled before it came into the lab. They were very calcified and were decaled for 1-3 days in Cal Rite. I do know they were not rinsed after decal and were put straight back into 10% NBF before I got them for processing. Should I have used a human control instead of canine? These were very large pieces that were crammed into the cassette. Thanks for the help. Sorry for the long post. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 1101 Bates St. Houston,TX 832-355=6524 (lab) 832-355-6812 (fax) Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | texasheartmedical.org | facebook | twitter CONFIDENTIALITY NOTICE: This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PfbeBCCAmug!wzfab7qYW4xTqIVaO5gZ8hZnKbB0B7NR6-rNUpGwMSKTNuo2Q6VczFcFIeDklobUHg$ ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PfbeBCCAmug!wzfab7qYW4xTqIVaO5gZ8hZnKbB0B7NR6-rNUpGwMSKTNuo2Q6VczFcFIeDklobUHg$ ------------------------------ End of Histonet Digest, Vol 210, Issue 6 **************************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=d6cee6RqBYY-X2zQVBgyPwvGIdeVt4xGBNORG-6pQQg&s=_1l6orXk4gnWz1brPJblJKVMfV-6UVaY3TQ5IuaDfKk&e= From relia1 at earthlink.net Fri May 21 10:07:40 2021 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 21 May 2021 11:07:40 -0400 Subject: [Histonet] OT but timely. Stressed Out? Just Breathe. Message-ID: <005901d74e53$0b591ac0$220b5040$@earthlink.net> Hi Histopeeps, This post is off topic but bear with me. I read this article and thought I would share it with everyone. If you can use it - fantastic if you can pass it along to someone else who needs it even better! Have a wonderful weekend. https://hbr.org/2020/09/research-why-breathing-is-so-effective-at-reducing-s tress?utm_medium=social&utm_campaign=hbr&utm_source=linkedin&tpcc=orgsocial_ edit&fbclid=IwAR2-FDmByfs0E3m9JQ3ZPkaM7S4Pt9IbhAEdJa7iwVN0bZ9ZANhNFcL4u6E Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From relia1 at earthlink.net Fri May 21 10:25:58 2021 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 21 May 2021 11:25:58 -0400 Subject: [Histonet] Repost OT Stressed out Just Breathe - correct link. Message-ID: <006601d74e55$9992f250$ccb8d6f0$@earthlink.net> Hi Histopeeps, Sorry for the multiple posts. Here is the link for the article on managing stress through breathing. https://hbr.org/2020/09/research-why-breathing-is-so-effective-at-reducing-s tress?utm_medium=social&utm_campaign=hbr&utm_source=linkedin&tpcc=orgsocial_ edit&fbclid=IwAR2-FDmByfs0E3m9JQ3ZPkaM7S4Pt9IbhAEdJa7iwVN0bZ9ZANhNFcL4u6E Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From jkiernan at uwo.ca Sat May 22 14:13:48 2021 From: jkiernan at uwo.ca (John Kiernan) Date: Sat, 22 May 2021 19:13:48 +0000 Subject: [Histonet] Luxol fast blue for staining myelin. Validity of sources. Message-ID: This Histonet query is addressed to vendors of stains (powders and/or solutions), and to anyone who makes up luxol fast blue in the lab, avoiding the high cost of buying and transporting the flammable ready-made staining solution. Most diagnostic labs buy ready-made staining solutions and follow a vendor's instructions, whereas research labs are likely to buy dye powders, make up their own solutions, and use them according to published books or papers. This means that the largest amounts of luxol fast blue (LFB) powder are sold to vendors of staining solutions. Am I right? Are vendors having any problems with getting dye powders called luxol fast blue (LFB) that are consistently OK for staining myelin sheaths of axons in sections of brain, spinal cord etc? The LFB name is informally attached to at least three dyes used as stains: LFB G (CI Solvent blue 34) and LFB ARN (CI Solvent blue37) are azo dyes, whereas LFB MBS (CI 74180, Solvent blue 38) is a copper phthalocyanine. All three have been used in similar (but not identical) published staining methods for myelin. LFB MBS is the dye most often used for this purpose, but not much is known about variation in the purity of commercial batches. It has many commercial uses and dozens of trade-names. See http://www.worlddyevariety.com/category/solvent-dyes. (It's free, unlike the Colour Index, but don't believe everything it says.) Luxol fast blue (whichever one) may or may not be the best stain for showing myelinated axons and regions of demyelination, but it probably is the dye most used for doing this job. Is there a need for independent third-party identification, testing and certification of LFB powders? If you have encountered a bad jar of powder labeled luxol fast blue, please reply, including if possible the name on the label and the source. The Biological Stain Commission (BSC) has never offered testing and certification for LFB as a stain for myelin, even though "luxol" dye have been in use for this job since 1953. Should third-party certification be made available for luxol fast blue? Would you submit samples for this service? For more information, see the link below. John Kiernan Secretary, Biological Stain Commission, Inc. https://biologicalstaincommission.org/for-vendors/ For Vendors and Users of Stains | The Biological Stain Commission Vendor FAQs: The Biological Stain Commission is a not-for-profit organization which is dedicated to the endorsement of histological materials. It is the mission of the BSC to ensure the quality of biological stains on the market that are sold by many US companies. biologicalstaincommission.org = = = From mssabater19 at gmail.com Wed May 26 13:28:00 2021 From: mssabater19 at gmail.com (Matthew Sabater) Date: Wed, 26 May 2021 11:28:00 -0700 Subject: [Histonet] Histotechnician Job Opportunity (Day Shift) -- San Francisco, CA Message-ID: SUTTER CALIFORNIA PACIFIC MEDICAL CENTER Job ID#: CPMC-2105689 Histotechnologist APPLY @ https://sutterhealth.taleo.net/careersection/sh/jobdetail.ftl?lang=en&job=CPMC-2105689 Description The Histotechnologist processes and stains tissue specimens; labels and files blocks and slides. This position participates in all quality and operation functions necessary for the Histology Department. EDUCATION: High school diploma or equivalent education/experience required. LICENSURES & CERTIFICATIONS: American Society of Clinical Pathologist - ASCP preferred. Certified Histotechnologist - CN-HISTO preferred. EXPERIENCE: Experience in a histopathology laboratory as typically acquired in one-year of one the job training required. SKILLS & KNOWLEDGE: Knowledge of software applications (such as MS Word and Excel) required. Basic knowledge of English language, grammar and composition. Ability to identify problems, prioritize and problem solve. Excellent interpersonal and customer service skills. Training skills required. Ability to maintain levels of inventory to assure availability. Basic math skills demonstrating accuracy and attention to details. Basic keyboarding and computer skills. Ability to gather data to complete reports on a timely basis. Ability to deal with problems involving a few concrete variables in standardized situations. Primary Location California-San Francisco-San Francisco If interested please contact Pathology Manager Matthew Sabater: sabatem at sutterhealth.org From Timothy.Morken at ucsf.edu Fri May 28 10:31:12 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 28 May 2021 15:31:12 +0000 Subject: [Histonet] IF with permanent mounting media? Message-ID: Has anyone tried using xylene/permanent mounting media for immunofluorescence stains? I had a question from a pathologist who wondered if we could do this. I have never heard of anyone doing it. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From paula at excaliburpathology.com Fri May 28 10:53:12 2021 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Fri, 28 May 2021 15:53:12 +0000 (UTC) Subject: [Histonet] IF with permanent mounting media? In-Reply-To: References: Message-ID: <1996829569.833583.1622217192240@mail.yahoo.com> Hi, you can use "Crystal Mount" first, let it dry, and then coverslip with xylene/resin. I do this routinely with AEC IHC and oil red O and other fat stains. Crystal Mount only on retinal flat mounts to be shipped out to others for future staining, as it can be removed with water, FYI: Crystal mount is no longer made under that name, but do a search on Electron Microscopy Sciences website and their version will come up in the results. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Friday, May 28, 2021, 10:43:39 AM CDT, Morken, Timothy via Histonet wrote: Has anyone tried using xylene/permanent mounting media for immunofluorescence stains? I had a question from a pathologist who wondered if we could do this. I have never heard of anyone doing it. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hclaiac at connect.ust.hk Fri May 28 10:53:52 2021 From: hclaiac at connect.ust.hk (Ho-chun LAI) Date: Fri, 28 May 2021 15:53:52 +0000 Subject: [Histonet] IF with permanent mounting media? In-Reply-To: References: Message-ID: Tim, Yes it is possible. I added a distilled water rinse and air dry step after the final wash so the slide became dehydrated enough to be mounted by DPX. JacksonImmuno does suggest Cy dyes for DPX mounts, but I found as long as you don't do the alcohol dehydration, alexa is far brighter. The fluorescent signal will indeed be much more long lived than temporary mount like mowiol, some of my first mounts (GAD67/GFAP) retains signal even after an entire year. But you should expect they would fade somewhere around 3 months. I realized I did not reply to the histonet the last time, I still need to get myself familiarized with this : ( John Lai Posdoctoral Fellow, School of Life Science, HKUST -----Original Message----- From: Morken, Timothy via Histonet Sent: Friday, May 28, 2021 11:31 PM To: Histonet Subject: [Histonet] IF with permanent mounting media? Has anyone tried using xylene/permanent mounting media for immunofluorescence stains? I had a question from a pathologist who wondered if we could do this. I have never heard of anyone doing it. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Fri May 28 10:57:18 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 28 May 2021 15:57:18 +0000 Subject: [Histonet] IF with permanent mounting media? In-Reply-To: References: Message-ID: Sounds interesting. How about with FITC label? Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Ho-chun LAI via Histonet Sent: Friday, May 28, 2021 8:54 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IF with permanent mounting media? Tim, Yes it is possible. I added a distilled water rinse and air dry step after the final wash so the slide became dehydrated enough to be mounted by DPX. JacksonImmuno does suggest Cy dyes for DPX mounts, but I found as long as you don't do the alcohol dehydration, alexa is far brighter. The fluorescent signal will indeed be much more long lived than temporary mount like mowiol, some of my first mounts (GAD67/GFAP) retains signal even after an entire year. But you should expect they would fade somewhere around 3 months. I realized I did not reply to the histonet the last time, I still need to get myself familiarized with this : ( John Lai Posdoctoral Fellow, School of Life Science, HKUST -----Original Message----- From: Morken, Timothy via Histonet Sent: Friday, May 28, 2021 11:31 PM To: Histonet Subject: [Histonet] IF with permanent mounting media? Has anyone tried using xylene/permanent mounting media for immunofluorescence stains? I had a question from a pathologist who wondered if we could do this. I have never heard of anyone doing it. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!60RRHLphVm7d74nChodBETeMNLNMTxDFxfKeOmzJbNfEFJKTdukuKJ0E1ZADKIgPcrjAqQ$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!60RRHLphVm7d74nChodBETeMNLNMTxDFxfKeOmzJbNfEFJKTdukuKJ0E1ZADKIgPcrjAqQ$ From hclaiac at connect.ust.hk Fri May 28 11:04:35 2021 From: hclaiac at connect.ust.hk (Ho-chun LAI) Date: Fri, 28 May 2021 16:04:35 +0000 Subject: [Histonet] IF with permanent mounting media? In-Reply-To: References: Message-ID: I personally did not tried FITC, but I'd used Cy dyes, Alexa Fluors, AF dyes as well as DAPI. I did not observe any harsh effect on fluorescence on those dyes. I'll guess FITC will also give good signal. John Lai Posdoctoral Fellow, School of Life Science, HKUST -----Original Message----- From: Morken, Timothy Sent: Friday, May 28, 2021 11:57 PM To: Ho-chun LAI Cc: Histonet Subject: RE: IF with permanent mounting media? Sounds interesting. How about with FITC label? Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Ho-chun LAI via Histonet Sent: Friday, May 28, 2021 8:54 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IF with permanent mounting media? Tim, Yes it is possible. I added a distilled water rinse and air dry step after the final wash so the slide became dehydrated enough to be mounted by DPX. JacksonImmuno does suggest Cy dyes for DPX mounts, but I found as long as you don't do the alcohol dehydration, alexa is far brighter. The fluorescent signal will indeed be much more long lived than temporary mount like mowiol, some of my first mounts (GAD67/GFAP) retains signal even after an entire year. But you should expect they would fade somewhere around 3 months. I realized I did not reply to the histonet the last time, I still need to get myself familiarized with this : ( John Lai Posdoctoral Fellow, School of Life Science, HKUST -----Original Message----- From: Morken, Timothy via Histonet Sent: Friday, May 28, 2021 11:31 PM To: Histonet Subject: [Histonet] IF with permanent mounting media? Has anyone tried using xylene/permanent mounting media for immunofluorescence stains? I had a question from a pathologist who wondered if we could do this. I have never heard of anyone doing it. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!60RRHLphVm7d74nChodBETeMNLNMTxDFxfKeOmzJbNfEFJKTdukuKJ0E1ZADKIgPcrjAqQ$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!60RRHLphVm7d74nChodBETeMNLNMTxDFxfKeOmzJbNfEFJKTdukuKJ0E1ZADKIgPcrjAqQ$ From sandra.cheasty at wisc.edu Fri May 28 13:37:42 2021 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Fri, 28 May 2021 18:37:42 +0000 Subject: [Histonet] Two University of Wisconsin Positions Message-ID: Hello, We have two positions open in anatomic pathology at the School of Veterinary Medicine at the University of Wisconsin, Madison, the links are below... https://jobs.hr.wisc.edu/en-us/job/509270/histology-technology-supervisor (application deadline June 10th) https://jobs.hr.wisc.edu/en-us/job/509189/anatomic-pathology-laboratory-manager (application deadline June 3rd) Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From jkiernan at uwo.ca Fri May 28 17:19:36 2021 From: jkiernan at uwo.ca (John Kiernan) Date: Fri, 28 May 2021 22:19:36 +0000 Subject: [Histonet] IF with permanent mounting media? In-Reply-To: References: Message-ID: Espada J, Juarranz A, Galaz S, Canete M, Villanueva A, Pacheco M, Stockert JC (2005) Non-aqueous permanent mounting for immunofluorescence microscopy. Histochem. Cell Biol. 123: 329-334. https://doi.org/10.1007/s00418-005-0769-2 A PDF can be downloaded from the Google Scholar entry for this article. John Kiernan London, Canada = = = ________________________________ From: Morken, Timothy via Histonet Sent: May 28, 2021 11:31 AM To: Histonet Subject: [Histonet] IF with permanent mounting media? Has anyone tried using xylene/permanent mounting media for immunofluorescence stains? I had a question from a pathologist who wondered if we could do this. I have never heard of anyone doing it. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Fri May 28 17:43:41 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 28 May 2021 22:43:41 +0000 Subject: [Histonet] IF with permanent mounting media? In-Reply-To: References: Message-ID: Thanks John! This looks interesting to say the least. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From: John Kiernan Sent: Friday, May 28, 2021 3:20 PM To: Histonet ; Morken, Timothy Subject: Re: IF with permanent mounting media? Espada J, Juarranz A, Galaz S, Canete M, Villanueva A, Pacheco M, Stockert JC (2005) Non-aqueous permanent mounting for immunofluorescence microscopy. Histochem. Cell Biol. 123: 329-334. https://doi.org/10.1007/s00418-005-0769-2 ? ? ? ? ? ? ? ? ? ? ZjQcmQRYFpfptBannerStart This Message Is From an External Sender This message came from outside your organization. ZjQcmQRYFpfptBannerEnd Espada J, Juarranz A, Galaz S, Canete M, Villanueva A, Pacheco M, Stockert JC (2005) Non-aqueous permanent mounting for immunofluorescence microscopy. Histochem. Cell Biol. 123: 329-334. https://doi.org/10.1007/s00418-005-0769-2 A PDF can be downloaded from the Google Scholar entry for this article. John Kiernan London, Canada = = = ________________________________ From: Morken, Timothy via Histonet > Sent: May 28, 2021 11:31 AM To: Histonet > Subject: [Histonet] IF with permanent mounting media? Has anyone tried using xylene/permanent mounting media for immunofluorescence stains? I had a question from a pathologist who wondered if we could do this. I have never heard of anyone doing it. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet