From relia1 at earthlink.net Tue Jun 1 11:15:48 2021 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 1 Jun 2021 12:15:48 -0400 Subject: [Histonet] Exciting Opportunities in CA and one in Dallas, TX to do something different with your Histology Skillz! Message-ID: <04f701d75701$628a8cf0$279fa6d0$@earthlink.net> Hello Histopeeps, I hope this is the start to a great week! I usually only contact you weekly but this absolutely amazing opportunity just came in and I thought I would see if you or anyone you know might be interested in hearing about it. This is a RELIA EXCLUSIVE!!! Full Time Permanent M-F Days!!! My client located in Dallas, Texas has engaged me to find them a talented histotech with strong skills in routine histology and IHC. You will be providing technical support to their customers. Histopeeps, this is an amazing opportunity to work onsite in a state of the art lab helping your fellow histopeeps! I also have exciting opportunities in: California in: Ventura San Diego Aliso Viejo Modesto And some great positions in: Virginia Florida Maryland And new opportunities coming in daily! My clients offer VERY competitive pay rates, excellent benefits, relocation/sign on bonuses and opportunity to grow. If you or anyone you know might be interested, please contact me. If I place someone you refer You will earn a referral fee! I can be reached toll free at the office at 866-607-3542 or relia1 at earthlink.net or you can always catch me ASAP on cell via call or text at 407-353-5070. Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From madeathridge at pastnashville.com Wed Jun 2 08:53:38 2021 From: madeathridge at pastnashville.com (Maryann Deathridge) Date: Wed, 2 Jun 2021 13:53:38 +0000 Subject: [Histonet] Fw: DIF on formalin fixed process tissue In-Reply-To: References: Message-ID: ________________________________________ From: Maryann Deathridge Sent: Wednesday, June 2, 2021 8:44 AM To: histonet at lists.utsouthwestern.edu> Subject: DIF on formalin fixed process tissue Histonetters: I know the answer but it "never hurt's to ask" Is there any means of salvaging a "DIF"tissue sample which was mistakenly processed as formalin fixed tissue. Any tips, thoughts or ideas welcomed! Last challenge before 44yr retirement 6/4/21 :) Thank you in advance... Maryann Deathridge, BS, HT(ASCP) (615) 298-4100 Lab Manager 4220 Harding Place, Suite 504 Nashville, TN 37205 From alida.bailleul at gmail.com Thu Jun 3 06:53:57 2021 From: alida.bailleul at gmail.com (Alida Bailleul) Date: Thu, 3 Jun 2021 19:53:57 +0800 Subject: [Histonet] Protocol for DAPI staining on paraffin sections In-Reply-To: References: Message-ID: Dear Histonet list, I have made some paraffin blocks of demineralized bone and cartilage. I would like to stain paraffin slides with DAPI (or another DNA stain). Does DAPI staining (or other fluorescent DNA stain) work well on paraffin sections? Does anyone have a protocol they would like to share? I am assuming that histological stains (like the Feulgen stain would work better?). I could also try both. Thank you in advance All the best Alida -- Dr. Alida M. Bailleul Associate Research Fellow/Associate Professor Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences www.ivpp-avianevolution.com & Research Associate of Paleontology, Museum of the Rockies, Montana State University Google Scholar - ResearchGate From madeathridge at pastnashville.com Thu Jun 3 09:53:12 2021 From: madeathridge at pastnashville.com (Maryann Deathridge) Date: Thu, 3 Jun 2021 14:53:12 +0000 Subject: [Histonet] Thank You EVERYONE for the "DIF" responses and Retirement Wishes Message-ID: Maryann Deathridge, BS, HT(ASCP) (615) 298-4100 Lab Manager 4220 Harding Place, Suite 504 Nashville, TN 37205 From relia1 at earthlink.net Thu Jun 3 10:52:23 2021 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 3 Jun 2021 11:52:23 -0400 Subject: [Histonet] RELIA HOT Leadership Opportunity in FL Brand new management role in a brand new lab!! A RELIA EXCLUSIVE!! Message-ID: <00ff01d75890$983ba6a0$c8b2f3e0$@earthlink.net> Hi Histopeeps, How are you? I hope you are having a great week! I have a very exciting opportunity to share and if you aren't interested maybe you know someone who might be! I have been engaged on a search by one of my very best clients located on the southwest coast of the state of Florida. My client needs a histology lab manager for a brand new lab facility. This is a newly created position. We need someone with strong histology skills and excellent leadership capabilities and experience. My client has made this position a TOP PRIORITY!! They are offering a fantastic compensation package including relocation, sign on and performance bonuses not to mention a very generous salary. The help I need from you Histopeeps is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them or pass their contact information to me? *remember if I place someone you refer to me you earn a referral bonus* This position is full time and permanent. And my client is ready to interview and hire!! If you are interested in this position please contact me ASAP On my cell/text 407-353-5070 or toll free at 866-607-3542 Or via email at relia1 at earthlink.net If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won't release it to anyone without your permission. Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From carl.hobbs at kcl.ac.uk Thu Jun 3 13:38:33 2021 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Thu, 3 Jun 2021 18:38:33 +0000 Subject: [Histonet] Re Protocol for DAPI staining on paraffin sections Message-ID: Use DAPI/HOECHST at the same concentration you use for cells/FS I use Hoechst H33258 Sigma 10mg/ml soln I dilute by adding 0.5 microL to 100 microL buffer then add 1/100 to Alexa secondaries ( final 1/20K diln factor) Some incubate in HOECHST or DAPI after secondary ab incubation, for IF Sure, if only "staining" nuclei, incubate in 1/20K Hoechst in same buffer This will vary for each lab Or, buy mounting medium that contains DAPI Good luck Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 From carl.hobbs at kcl.ac.uk Fri Jun 4 11:34:00 2021 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 4 Jun 2021 16:34:00 +0000 Subject: [Histonet] Re Protocol for DAPI staining on paraffin sections Message-ID: Dear Alida, That is a good point. I probably have only done HOECHST on Pwax sections after they have been heat-Antigen retrieved ( HIER) However, H ( or DAPI) works on fixed cell monolayers: they don't get HIER treatment) Nor do frozen sections ( well, some do) and the HOECHST/DAPI works fine on both However, perhaps you can just take a Pwax section to water and apply HOECHST for 10 mins, wash off, mount in anti fade aq. mountant and have a look down a Fluorescence scope? If you can wait, I will check this next Monday ( put H on a non HIER'd Pwax section and get back to you by Monday later) Unless, whoever that is doing the fluorescence DAPI/HOECHST nuclear staining is actually doing it on Pwax sections that have been HIER-ed? Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6810 From Nancy.Schmitt at mercyhealth.com Fri Jun 4 12:13:35 2021 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Fri, 4 Jun 2021 17:13:35 +0000 Subject: [Histonet] autopsy blocks Message-ID: Hello- Can you tell me what you are doing with autopsy blocks? How long are keeping these blocks/slides? Appreciated, Nancy Dubuque, IA Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From bakevictoria at gmail.com Fri Jun 4 15:14:53 2021 From: bakevictoria at gmail.com (Victoria Baker) Date: Fri, 4 Jun 2021 16:14:53 -0400 Subject: [Histonet] autopsy blocks In-Reply-To: References: Message-ID: Hi! We are required to keep all of our materials for 20 years. CAP I think is less, but we have to follow NYS. Vikki Baker On Fri, Jun 4, 2021 at 1:22 PM Nancy Schmitt via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello- > > Can you tell me what you are doing with autopsy blocks? > How long are keeping these blocks/slides? > Appreciated, > Nancy > Dubuque, IA > > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jkiernan at uwo.ca Sat Jun 5 14:30:06 2021 From: jkiernan at uwo.ca (John Kiernan) Date: Sat, 5 Jun 2021 19:30:06 +0000 Subject: [Histonet] Protocol for DAPI staining on paraffin sections In-Reply-To: References: , Message-ID: Not everyone knows that the product of the Feulgen reaction is fluorescent. If you do the method on paraffin sections, DNA in nuclear chromatin is red with ordinary illumination. If you look with a fluorescence microscope (blue excitation) it shows as a brownish-red fluorescence in the chromatin. A similar fluorescent colour is seen in conventional PAS-stained sections, in mucus, basement membranes, etc. You need a non-fluorescent mounting medium: DPX or one of the various poly(methyl methacrylate)-based media such as Entellan. John Kiernan Professor Emeritus, Dept of Anatomy & Cell Biology University of Western Ontario, London, Canada https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html Also Secretary, Biological Stain Commission, Inc. https://biologicalstaincommission.org = = = ________________________________ From: Alida Bailleul via Histonet Sent: June 3, 2021 7:53 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Protocol for DAPI staining on paraffin sections Dear Histonet list, I have made some paraffin blocks of demineralized bone and cartilage. I would like to stain paraffin slides with DAPI (or another DNA stain). Does DAPI staining (or other fluorescent DNA stain) work well on paraffin sections? Does anyone have a protocol they would like to share? I am assuming that histological stains (like the Feulgen stain would work better?). I could also try both. Thank you in advance All the best Alida -- Dr. Alida M. Bailleul Associate Research Fellow/Associate Professor Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences www.ivpp-avianevolution.com & Research Associate of Paleontology, Museum of the Rockies, Montana State University Google Scholar - ResearchGate _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmacdonald at mtsac.edu Sat Jun 5 23:33:33 2021 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Sun, 6 Jun 2021 04:33:33 +0000 Subject: [Histonet] Prussian Blue Reaction In-Reply-To: References: Message-ID: Does anyone know of a way to remove/reduce the Prussian blue reaction? Thanks, Jennifer From gu.lang at gmx.at Sun Jun 6 04:18:03 2021 From: gu.lang at gmx.at (Gudrun Lang) Date: Sun, 6 Jun 2021 11:18:03 +0200 Subject: [Histonet] Prussian Blue Reaction In-Reply-To: References: Message-ID: <000001d75ab4$db366ff0$91a34fd0$@gmx.at> Hi Jennifer, Why do you want to reduce the staining? I ask, because the impact of hydrochloric acid on the tissue may influence the following results anyway. I think, that the prussian blue pigment cannot be removed in an easy way. It is resistent to solvents and mineral acids. http://www.epsilonpigments.com/inorganic-pigment/prussian-blue/Prussian-Blue -for-Solvent-Based-Inks.html On the other hand, if the blue colour doesn't interfere with your following staining, you can try to simple make a "double stain". Regards Gudrun -----Urspr?ngliche Nachricht----- Von: Mac Donald, Jennifer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Sonntag, 6. Juni 2021 06:34 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Prussian Blue Reaction Does anyone know of a way to remove/reduce the Prussian blue reaction? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDISBROW at msn.com Sun Jun 6 19:34:24 2021 From: CDISBROW at msn.com (Carrie Disbrow) Date: Mon, 7 Jun 2021 00:34:24 +0000 Subject: [Histonet] Autopsy blocks and slides Message-ID: Autopsy per CAP is ten years block storage for both regular and forensic. Forensic slides must be kept thirty years. Found that info with a Google search. Get Outlook for Android From jmacdonald at mtsac.edu Sun Jun 6 23:47:15 2021 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Mon, 7 Jun 2021 04:47:15 +0000 Subject: [Histonet] Prussian Blue Reaction In-Reply-To: <000001d75ab4$db366ff0$91a34fd0$@gmx.at> References: <000001d75ab4$db366ff0$91a34fd0$@gmx.at> Message-ID: The tissue was overstained and the blue was interfering with interpretation -----Original Message----- From: Gudrun Lang Sent: Sunday, June 6, 2021 2:18 AM To: Mac Donald, Jennifer Cc: histonet at lists.utsouthwestern.edu Subject: AW: [Histonet] Prussian Blue Reaction EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Hi Jennifer, Why do you want to reduce the staining? I ask, because the impact of hydrochloric acid on the tissue may influence the following results anyway. I think, that the prussian blue pigment cannot be removed in an easy way. It is resistent to solvents and mineral acids. https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.epsilonpigments.com%2Finorganic-pigment%2Fprussian-blue%2FPrussian-Blue&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C0fbc82a2b13749a4222608d928cbfe52%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637585679205067185%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=KjvijcfrVPGZKGsAn6qX5rMKtulHpmsAzqHEkwz%2B96Y%3D&reserved=0 -for-Solvent-Based-Inks.html On the other hand, if the blue colour doesn't interfere with your following staining, you can try to simple make a "double stain". Regards Gudrun -----Urspr?ngliche Nachricht----- Von: Mac Donald, Jennifer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Sonntag, 6. Juni 2021 06:34 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Prussian Blue Reaction Does anyone know of a way to remove/reduce the Prussian blue reaction? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C0fbc82a2b13749a4222608d928cbfe52%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637585679205067185%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=gRrUmRDEU3BfcA0rEgQOgBvPHIQ05IRM6WozVZiiR1g%3D&reserved=0 From jkiernan at uwo.ca Mon Jun 7 11:50:18 2021 From: jkiernan at uwo.ca (John Kiernan) Date: Mon, 7 Jun 2021 16:50:18 +0000 Subject: [Histonet] Prussian Blue Reaction In-Reply-To: References: <000001d75ab4$db366ff0$91a34fd0$@gmx.at>, Message-ID: Overstained? Doesn't that mean the tissue contains a lot of iron and you are seeing where it is - which was the reason for doing Prussian blue histochemistry. Gudrun Lang correctly says that mineral acids won't remove it. Oxalic acid is said to dissolve Prussian blue (? by chelation); I've never tried this. If it works, you will no longer see where the iron is. To see features other than the distribution of iron, why not just stain another section from the block with a general-purpose stain like Giemsa or H&E? John Kiernan London, Canada = = = ________________________________ From: Mac Donald, Jennifer via Histonet Sent: June 7, 2021 12:47 AM To: Gudrun Lang Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Prussian Blue Reaction The tissue was overstained and the blue was interfering with interpretation -----Original Message----- From: Gudrun Lang Sent: Sunday, June 6, 2021 2:18 AM To: Mac Donald, Jennifer Cc: histonet at lists.utsouthwestern.edu Subject: AW: [Histonet] Prussian Blue Reaction EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Hi Jennifer, Why do you want to reduce the staining? I ask, because the impact of hydrochloric acid on the tissue may influence the following results anyway. I think, that the prussian blue pigment cannot be removed in an easy way. It is resistent to solvents and mineral acids. https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.epsilonpigments.com%2Finorganic-pigment%2Fprussian-blue%2FPrussian-Blue&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C0fbc82a2b13749a4222608d928cbfe52%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637585679205067185%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=KjvijcfrVPGZKGsAn6qX5rMKtulHpmsAzqHEkwz%2B96Y%3D&reserved=0 -for-Solvent-Based-Inks.html On the other hand, if the blue colour doesn't interfere with your following staining, you can try to simple make a "double stain". Regards Gudrun -----Urspr?ngliche Nachricht----- Von: Mac Donald, Jennifer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Sonntag, 6. Juni 2021 06:34 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Prussian Blue Reaction Does anyone know of a way to remove/reduce the Prussian blue reaction? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C0fbc82a2b13749a4222608d928cbfe52%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637585679205067185%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=gRrUmRDEU3BfcA0rEgQOgBvPHIQ05IRM6WozVZiiR1g%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmacdonald at mtsac.edu Mon Jun 7 11:58:19 2021 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Mon, 7 Jun 2021 16:58:19 +0000 Subject: [Histonet] Prussian Blue Reaction In-Reply-To: References: <000001d75ab4$db366ff0$91a34fd0$@gmx.at>, Message-ID: The instrument malfunction and it was overstained. From: John Kiernan Sent: Monday, June 7, 2021 9:50 AM To: Gudrun Lang ; Mac Donald, Jennifer Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Prussian Blue Reaction EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Overstained? Doesn't that mean the tissue contains a lot of iron and you are seeing where it is - which was the reason for doing Prussian blue histochemistry. Gudrun Lang correctly says that mineral acids won't remove it. Oxalic acid is said to dissolve Prussian blue (? by chelation); I've never tried this. If it works, you will no longer see where the iron is. To see features other than the distribution of iron, why not just stain another section from the block with a general-purpose stain like Giemsa or H&E? John Kiernan London, Canada = = = ________________________________ From: Mac Donald, Jennifer via Histonet > Sent: June 7, 2021 12:47 AM To: Gudrun Lang > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Prussian Blue Reaction The tissue was overstained and the blue was interfering with interpretation -----Original Message----- From: Gudrun Lang > Sent: Sunday, June 6, 2021 2:18 AM To: Mac Donald, Jennifer > Cc: histonet at lists.utsouthwestern.edu Subject: AW: [Histonet] Prussian Blue Reaction EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Hi Jennifer, Why do you want to reduce the staining? I ask, because the impact of hydrochloric acid on the tissue may influence the following results anyway. I think, that the prussian blue pigment cannot be removed in an easy way. It is resistent to solvents and mineral acids. https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.epsilonpigments.com%2Finorganic-pigment%2Fprussian-blue%2FPrussian-Blue&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C0fbc82a2b13749a4222608d928cbfe52%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637585679205067185%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=KjvijcfrVPGZKGsAn6qX5rMKtulHpmsAzqHEkwz%2B96Y%3D&reserved=0 -for-Solvent-Based-Inks.html On the other hand, if the blue colour doesn't interfere with your following staining, you can try to simple make a "double stain". Regards Gudrun -----Urspr?ngliche Nachricht----- Von: Mac Donald, Jennifer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Sonntag, 6. Juni 2021 06:34 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Prussian Blue Reaction Does anyone know of a way to remove/reduce the Prussian blue reaction? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C0fbc82a2b13749a4222608d928cbfe52%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637585679205067185%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=gRrUmRDEU3BfcA0rEgQOgBvPHIQ05IRM6WozVZiiR1g%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren at gmail.com Mon Jun 7 15:35:29 2021 From: jaylundgren at gmail.com (Jay Lundgren) Date: Mon, 7 Jun 2021 15:35:29 -0500 Subject: [Histonet] Prussian Blue Reaction In-Reply-To: References: <000001d75ab4$db366ff0$91a34fd0$@gmx.at> Message-ID: Supposed to be insoluble. Try potassium permanganate followed by oxalaic acid. But book says insoluble. On Monday, June 7, 2021, Mac Donald, Jennifer via Histonet < histonet at lists.utsouthwestern.edu> wrote: > The instrument malfunction and it was overstained. > > From: John Kiernan > Sent: Monday, June 7, 2021 9:50 AM > To: Gudrun Lang ; Mac Donald, Jennifer < > jmacdonald at mtsac.edu> > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Prussian Blue Reaction > > EXTERNAL SENDER- Exercise caution with requests, links, and attachments. > Overstained? Doesn't that mean the tissue contains a lot of iron and you > are seeing where it is - which was the reason for doing Prussian blue > histochemistry. Gudrun Lang correctly says that mineral acids won't remove > it. Oxalic acid is said to dissolve Prussian blue (? by chelation); I've > never tried this. If it works, you will no longer see where the iron is. To > see features other than the distribution of iron, why not just stain > another section from the block with a general-purpose stain like Giemsa or > H&E? > John Kiernan > London, Canada > = = = > ________________________________ > From: Mac Donald, Jennifer via Histonet > > Sent: June 7, 2021 12:47 AM > To: Gudrun Lang > > Cc: histonet at lists.utsouthwestern.edu utsouthwestern.edu> onet at lists.utsouthwestern.edu>> > Subject: Re: [Histonet] Prussian Blue Reaction > > The tissue was overstained and the blue was interfering with interpretation > > -----Original Message----- > From: Gudrun Lang > > Sent: Sunday, June 6, 2021 2:18 AM > To: Mac Donald, Jennifer >> > Cc: histonet at lists.utsouthwestern.edu utsouthwestern.edu> > Subject: AW: [Histonet] Prussian Blue Reaction > > EXTERNAL SENDER- Exercise caution with requests, links, and attachments. > > Hi Jennifer, > Why do you want to reduce the staining? > > I ask, because the impact of hydrochloric acid on the tissue may influence > the following results anyway. > I think, that the prussian blue pigment cannot be removed in an easy way. > It is resistent to solvents and mineral acids. > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww. > epsilonpigments.com%2Finorganic-pigment%2Fprussian-blue%2FPrussian- > Blue&data=04%7C01%7Cjmacdonald%40mtsac.edu% > 7C0fbc82a2b13749a4222608d928cbfe52%7Ccc4d4bf20a9e4240aedea7d1d688 > f935%7C0%7C0%7C637585679205067185%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAw > MDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata= > KjvijcfrVPGZKGsAn6qX5rMKtulHpmsAzqHEkwz%2B96Y%3D&reserved=0< > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww. > epsilonpigments.com%2Finorganic-pigment%2Fprussian-blue%2FPrussian- > Blue&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C2eeb915058594b1d14b508d929d4 > 55c4%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637586814671423758% > 7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik > 1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=xKy3v4qvO4spk0zrRxXWZfcsHWxbQC > %2FIQ1FbpMB390Y%3D&reserved=0> > -for-Solvent-Based-Inks.html > > On the other hand, if the blue colour doesn't interfere with your > following staining, you can try to simple make a "double stain". > > Regards > Gudrun > > -----Urspr?ngliche Nachricht----- > Von: Mac Donald, Jennifer via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > Gesendet: Sonntag, 6. Juni 2021 06:34 > An: histonet at lists.utsouthwestern.edu utsouthwestern.edu> > Betreff: [Histonet] Prussian Blue Reaction > > > Does anyone know of a way to remove/reduce the Prussian blue reaction? > Thanks, > Jennifer > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists. > utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet& > data=04%7C01%7Cjmacdonald%40mtsac.edu%7C0fbc82a2b13749a4222608d928cbfe52% > 7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637585679205067185%7CUnknown% > 7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik > 1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=gRrUmRDEU3BfcA0rEgQOgBvPHIQ05I > RM6WozVZiiR1g%3D&reserved=0 protection.outlook.com/?url=http%3A%2F%2Flists. > utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04% > 7C01%7Cjmacdonald%40mtsac.edu%7C2eeb915058594b1d14b508d929d455c4% > 7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637586814671433754%7CUnknown% > 7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik > 1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=1p0zF%2BbxdarMOeh8R% > 2B2HN3JrGroGYcbuplEXFxO6y1s%3D&reserved=0> > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet< > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists. > utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04% > 7C01%7Cjmacdonald%40mtsac.edu%7C2eeb915058594b1d14b508d929d455c4% > 7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637586814671443749%7CUnknown% > 7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik > 1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=%2FoXTqTKQY18uiQ%2BldVcTbiTv% > 2FA9bJGdcM4BfODypIC4%3D&reserved=0> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jmacdonald at mtsac.edu Mon Jun 7 15:41:11 2021 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Mon, 7 Jun 2021 20:41:11 +0000 Subject: [Histonet] Prussian Blue Reaction In-Reply-To: References: <000001d75ab4$db366ff0$91a34fd0$@gmx.at> Message-ID: Thank you. From: Jay Lundgren Sent: Monday, June 7, 2021 1:35 PM To: Mac Donald, Jennifer Cc: John Kiernan ; Gudrun Lang ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Prussian Blue Reaction EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Supposed to be insoluble. Try potassium permanganate followed by oxalaic acid. But book says insoluble. On Monday, June 7, 2021, Mac Donald, Jennifer via Histonet > wrote: The instrument malfunction and it was overstained. From: John Kiernan > Sent: Monday, June 7, 2021 9:50 AM To: Gudrun Lang >; Mac Donald, Jennifer > Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Prussian Blue Reaction EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Overstained? Doesn't that mean the tissue contains a lot of iron and you are seeing where it is - which was the reason for doing Prussian blue histochemistry. Gudrun Lang correctly says that mineral acids won't remove it. Oxalic acid is said to dissolve Prussian blue (? by chelation); I've never tried this. If it works, you will no longer see where the iron is. To see features other than the distribution of iron, why not just stain another section from the block with a general-purpose stain like Giemsa or H&E? John Kiernan London, Canada = = = ________________________________ From: Mac Donald, Jennifer via Histonet >> Sent: June 7, 2021 12:47 AM To: Gudrun Lang >> Cc: histonet at lists.utsouthwestern.edu> >> Subject: Re: [Histonet] Prussian Blue Reaction The tissue was overstained and the blue was interfering with interpretation -----Original Message----- From: Gudrun Lang >> Sent: Sunday, June 6, 2021 2:18 AM To: Mac Donald, Jennifer >> Cc: histonet at lists.utsouthwestern.edu> Subject: AW: [Histonet] Prussian Blue Reaction EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Hi Jennifer, Why do you want to reduce the staining? I ask, because the impact of hydrochloric acid on the tissue may influence the following results anyway. I think, that the prussian blue pigment cannot be removed in an easy way. It is resistent to solvents and mineral acids. https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.epsilonpigments.com%2Finorganic-pigment%2Fprussian-blue%2FPrussian-Blue&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C0fbc82a2b13749a4222608d928cbfe52%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637585679205067185%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=KjvijcfrVPGZKGsAn6qX5rMKtulHpmsAzqHEkwz%2B96Y%3D&reserved=0> -for-Solvent-Based-Inks.html On the other hand, if the blue colour doesn't interfere with your following staining, you can try to simple make a "double stain". Regards Gudrun -----Urspr?ngliche Nachricht----- Von: Mac Donald, Jennifer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Sonntag, 6. Juni 2021 06:34 An: histonet at lists.utsouthwestern.edu> Betreff: [Histonet] Prussian Blue Reaction Does anyone know of a way to remove/reduce the Prussian blue reaction? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu> https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C0fbc82a2b13749a4222608d928cbfe52%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637585679205067185%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=gRrUmRDEU3BfcA0rEgQOgBvPHIQ05IRM6WozVZiiR1g%3D&reserved=0> _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegghm at hotmail.com Mon Jun 7 17:09:32 2021 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Mon, 7 Jun 2021 22:09:32 +0000 Subject: [Histonet] Histonet Digest, Vol 211, Issue 7 In-Reply-To: References: Message-ID: The blue stain from prussian blue is a chemical reaction and indicates that there is a lot of iron in the section, you would not want it not to stain some of the iron there would you? I agree with John, take an adjacent section for a general stain. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 39037 N 11th Ave Phoenix, AZ 85086 C 720-281-5406 pruegghm at hotmail.com Doug Ruegg C720-281-5407 douglasr19 at hotmail.com ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Monday, June 7, 2021 11:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 211, Issue 7 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From tony.henwood at health.nsw.gov.au Mon Jun 7 18:24:41 2021 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon, 7 Jun 2021 23:24:41 +0000 Subject: [Histonet] Prussian Blue Reaction In-Reply-To: References: <000001d75ab4$db366ff0$91a34fd0$@gmx.at> Message-ID: <1f1d63b098cc4d9ebe26173a709a974d@SVDCMBX-MEX024.nswhealth.net> The following might be useful: Iron Histochemistry - A Review It is convenient to divide iron-containing complexes in human tissues into two categories: those in which the iron is loosely bound to proteins and easily released by mild acid treatment (eg hemosiderin) and those in which the iron is more strongly bound (masked iron) and cannot be released by mild acid hydrolysis (eg haemoglobin) (1). Iron in the body is stored in the forms of hemosiderin (ferric hydroxide polymer) or ferritin (a ferrous iron-protein complex) (1). Iron in tissues occurs mainly in the ferric state (2,3). The reactions that have been used for the detection of iron in tissues include (2-5): 1. The Quincke reaction using ammonium sulphide 2. The Perls reaction, using ferrocyanide, for ferric and the Turnbull Blue reaction, using ferricyanide for ferrous iron. 3. Coloured lakes, eg haematoxylin (Mallory's Method) 4. Coloured precipitates with organic chemicals not classified as dyes (eg bathophenanthroline). Ferric iron may be converted into ferrous iron by ammonium sulphide (Quinke's reaction) and the ferrous sulphide thus formed can then be demonstrated using the Turnbull blue reaction (3,5). Some iron-containing compounds (hemoglobin, malaria pigment, formalin pigment) do not react with the Perl's method because the iron is present in bound form. These compounds can be unmasked using hydrogen peroxide and then demonstrated using the Perl's reaction (1). Interestingly, it is possible to remove excess iron pigment from tissue sections. Iron can be removed by (5): . 15 min in 1% sodium dithionite in 0.1M acetate-HCl buffer (pH 4.5) . 3 hours in 2.4N HCl . 30 min in 3.7N H2SO4 . 15 min in 5% Oxalic acid Heavily pigmented tissues may need to have these times extended (5). References 1. Barka, T., Anderson, P.J., (1963) "Histochemistry: Theory, practice and bibliography" Harper & Row Publishers Inc, New York, p172-174. 2. Davenport, H.A., (1961) "Histological and Histochemical Technics" W.B. Saunders Co., Philadelphia, 280-284. 3. Gabe, M., (1976) "Histological Techniques" Masson, Paris, p311-317. 4. Lynch, M.J., Raphael, S., etal "Medical Laboratory Technology and Clinical Pathology" 2nd Ed, W.B Saunder Co, Philadelphia, p1135-1136. 5. Morton, D., (1978) "A comparison of iron histochemical methods for use on glycol methacrylate embedded tissues" Stain Tech 53(4):217-223. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Mac Donald, Jennifer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, 8 June 2021 6:41 AM To: Jay Lundgren Cc: histonet at lists.utsouthwestern.edu; John Kiernan Subject: Re: [Histonet] Prussian Blue Reaction Thank you. From: Jay Lundgren Sent: Monday, June 7, 2021 1:35 PM To: Mac Donald, Jennifer Cc: John Kiernan ; Gudrun Lang ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Prussian Blue Reaction EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Supposed to be insoluble. Try potassium permanganate followed by oxalaic acid. But book says insoluble. On Monday, June 7, 2021, Mac Donald, Jennifer via Histonet > wrote: The instrument malfunction and it was overstained. From: John Kiernan > Sent: Monday, June 7, 2021 9:50 AM To: Gudrun Lang >; Mac Donald, Jennifer > Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Prussian Blue Reaction EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Overstained? Doesn't that mean the tissue contains a lot of iron and you are seeing where it is - which was the reason for doing Prussian blue histochemistry. Gudrun Lang correctly says that mineral acids won't remove it. Oxalic acid is said to dissolve Prussian blue (? by chelation); I've never tried this. If it works, you will no longer see where the iron is. To see features other than the distribution of iron, why not just stain another section from the block with a general-purpose stain like Giemsa or H&E? John Kiernan London, Canada = = = ________________________________ From: Mac Donald, Jennifer via Histonet >> Sent: June 7, 2021 12:47 AM To: Gudrun Lang >> Cc: histonet at lists.utsouthwestern.edu> >> Subject: Re: [Histonet] Prussian Blue Reaction The tissue was overstained and the blue was interfering with interpretation -----Original Message----- From: Gudrun Lang >> Sent: Sunday, June 6, 2021 2:18 AM To: Mac Donald, Jennifer >> Cc: histonet at lists.utsouthwestern.edu> Subject: AW: [Histonet] Prussian Blue Reaction EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Hi Jennifer, Why do you want to reduce the staining? I ask, because the impact of hydrochloric acid on the tissue may influence the following results anyway. I think, that the prussian blue pigment cannot be removed in an easy way. It is resistent to solvents and mineral acids. https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.epsilonpigments.com%2Finorganic-pigment%2Fprussian-blue%2FPrussian-Blue&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C0fbc82a2b13749a4222608d928cbfe52%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637585679205067185%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=KjvijcfrVPGZKGsAn6qX5rMKtulHpmsAzqHEkwz%2B96Y%3D&reserved=0> -for-Solvent-Based-Inks.html On the other hand, if the blue colour doesn't interfere with your following staining, you can try to simple make a "double stain". Regards Gudrun -----Urspr?ngliche Nachricht----- Von: Mac Donald, Jennifer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Sonntag, 6. Juni 2021 06:34 An: histonet at lists.utsouthwestern.edu> Betreff: [Histonet] Prussian Blue Reaction Does anyone know of a way to remove/reduce the Prussian blue reaction? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu> https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C0fbc82a2b13749a4222608d928cbfe52%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637585679205067185%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=gRrUmRDEU3BfcA0rEgQOgBvPHIQ05IRM6WozVZiiR1g%3D&reserved=0> _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From jmacdonald at mtsac.edu Mon Jun 7 19:57:23 2021 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Tue, 8 Jun 2021 00:57:23 +0000 Subject: [Histonet] Prussian Blue Reaction In-Reply-To: <1f1d63b098cc4d9ebe26173a709a974d@SVDCMBX-MEX024.nswhealth.net> References: <000001d75ab4$db366ff0$91a34fd0$@gmx.at> <1f1d63b098cc4d9ebe26173a709a974d@SVDCMBX-MEX024.nswhealth.net> Message-ID: Thank you Tony. -----Original Message----- From: Tony Henwood (SCHN) Sent: Monday, June 7, 2021 4:25 PM To: Mac Donald, Jennifer ; Jay Lundgren Cc: John Kiernan ; 'histonet at lists.utsouthwestern.edu' Subject: RE: [Histonet] Prussian Blue Reaction EXTERNAL SENDER- Exercise caution with requests, links, and attachments. The following might be useful: Iron Histochemistry - A Review It is convenient to divide iron-containing complexes in human tissues into two categories: those in which the iron is loosely bound to proteins and easily released by mild acid treatment (eg hemosiderin) and those in which the iron is more strongly bound (masked iron) and cannot be released by mild acid hydrolysis (eg haemoglobin) (1). Iron in the body is stored in the forms of hemosiderin (ferric hydroxide polymer) or ferritin (a ferrous iron-protein complex) (1). Iron in tissues occurs mainly in the ferric state (2,3). The reactions that have been used for the detection of iron in tissues include (2-5): 1. The Quincke reaction using ammonium sulphide 2. The Perls reaction, using ferrocyanide, for ferric and the Turnbull Blue reaction, using ferricyanide for ferrous iron. 3. Coloured lakes, eg haematoxylin (Mallory's Method) 4. Coloured precipitates with organic chemicals not classified as dyes (eg bathophenanthroline). Ferric iron may be converted into ferrous iron by ammonium sulphide (Quinke's reaction) and the ferrous sulphide thus formed can then be demonstrated using the Turnbull blue reaction (3,5). Some iron-containing compounds (hemoglobin, malaria pigment, formalin pigment) do not react with the Perl's method because the iron is present in bound form. These compounds can be unmasked using hydrogen peroxide and then demonstrated using the Perl's reaction (1). Interestingly, it is possible to remove excess iron pigment from tissue sections. Iron can be removed by (5): . 15 min in 1% sodium dithionite in 0.1M acetate-HCl buffer (pH 4.5) . 3 hours in 2.4N HCl . 30 min in 3.7N H2SO4 . 15 min in 5% Oxalic acid Heavily pigmented tissues may need to have these times extended (5). References 1. Barka, T., Anderson, P.J., (1963) "Histochemistry: Theory, practice and bibliography" Harper & Row Publishers Inc, New York, p172-174. 2. Davenport, H.A., (1961) "Histological and Histochemical Technics" W.B. Saunders Co., Philadelphia, 280-284. 3. Gabe, M., (1976) "Histological Techniques" Masson, Paris, p311-317. 4. Lynch, M.J., Raphael, S., etal "Medical Laboratory Technology and Clinical Pathology" 2nd Ed, W.B Saunder Co, Philadelphia, p1135-1136. 5. Morton, D., (1978) "A comparison of iron histochemical methods for use on glycol methacrylate embedded tissues" Stain Tech 53(4):217-223. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Mac Donald, Jennifer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, 8 June 2021 6:41 AM To: Jay Lundgren Cc: histonet at lists.utsouthwestern.edu; John Kiernan Subject: Re: [Histonet] Prussian Blue Reaction Thank you. From: Jay Lundgren Sent: Monday, June 7, 2021 1:35 PM To: Mac Donald, Jennifer Cc: John Kiernan ; Gudrun Lang ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Prussian Blue Reaction EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Supposed to be insoluble. Try potassium permanganate followed by oxalaic acid. But book says insoluble. On Monday, June 7, 2021, Mac Donald, Jennifer via Histonet > wrote: The instrument malfunction and it was overstained. From: John Kiernan > Sent: Monday, June 7, 2021 9:50 AM To: Gudrun Lang >; Mac Donald, Jennifer > Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Prussian Blue Reaction EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Overstained? Doesn't that mean the tissue contains a lot of iron and you are seeing where it is - which was the reason for doing Prussian blue histochemistry. Gudrun Lang correctly says that mineral acids won't remove it. Oxalic acid is said to dissolve Prussian blue (? by chelation); I've never tried this. If it works, you will no longer see where the iron is. To see features other than the distribution of iron, why not just stain another section from the block with a general-purpose stain like Giemsa or H&E? John Kiernan London, Canada = = = ________________________________ From: Mac Donald, Jennifer via Histonet >> Sent: June 7, 2021 12:47 AM To: Gudrun Lang >> Cc: histonet at lists.utsouthwestern.edu> >> Subject: Re: [Histonet] Prussian Blue Reaction The tissue was overstained and the blue was interfering with interpretation -----Original Message----- From: Gudrun Lang >> Sent: Sunday, June 6, 2021 2:18 AM To: Mac Donald, Jennifer >> Cc: histonet at lists.utsouthwestern.edu> Subject: AW: [Histonet] Prussian Blue Reaction EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Hi Jennifer, Why do you want to reduce the staining? I ask, because the impact of hydrochloric acid on the tissue may influence the following results anyway. I think, that the prussian blue pigment cannot be removed in an easy way. It is resistent to solvents and mineral acids. https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.epsilonpigments.com%2Finorganic-pigment%2Fprussian-blue%2FPrussian-Blue&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C577f8cd8737f4691d4d408d92a0bbead%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637587052207325654%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=mON25kRZV0zqiRcFEcU9uNAi6P%2BiqIjkYcbhW4bOTRg%3D&reserved=0> -for-Solvent-Based-Inks.html On the other hand, if the blue colour doesn't interfere with your following staining, you can try to simple make a "double stain". Regards Gudrun -----Urspr?ngliche Nachricht----- Von: Mac Donald, Jennifer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Sonntag, 6. Juni 2021 06:34 An: histonet at lists.utsouthwestern.edu> Betreff: [Histonet] Prussian Blue Reaction Does anyone know of a way to remove/reduce the Prussian blue reaction? Thanks, Jennifer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu> https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C577f8cd8737f4691d4d408d92a0bbead%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637587052207335724%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=eLlGLeyeQlp2GuTUf4rKfnxTdDtd2xo4NufE%2F%2Fk2Y5w%3D&reserved=0> _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu> https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C577f8cd8737f4691d4d408d92a0bbead%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637587052207345631%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=xgOyi63vizFLhezv2qHQIFVINCA2xHnsioXMRPDGIt0%3D&reserved=0> _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C577f8cd8737f4691d4d408d92a0bbead%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637587052207345631%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=xgOyi63vizFLhezv2qHQIFVINCA2xHnsioXMRPDGIt0%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Cjmacdonald%40mtsac.edu%7C577f8cd8737f4691d4d408d92a0bbead%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C637587052207355951%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=8lusOF%2F8WQT9yYsHLfl46ukde10bEugXWVvQVWFvCuY%3D&reserved=0 This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From cxk340 at case.edu Tue Jun 8 09:57:24 2021 From: cxk340 at case.edu (Chaitanya Kolluru) Date: Tue, 8 Jun 2021 10:57:24 -0400 Subject: [Histonet] Issue with Technovit 7100 - nerve samples Message-ID: Hi, I'm working on a protocol to embed human nerve samples (about 3 mm in diameter, 5-7 mm in length) in glycol methacrylate and cut 2-3 micron thick sections. The sectioning seems fine at the start, but as I section deeper into the sample, I find a lot of gaps forming in between the fascicles, which I'm assuming are air bubbles. I'm attaching an image of the block surface after sectioning about 500 microns into the sample. I'm also attaching a text file of my protocol if that can help troubleshoot the issue. Any help on how I might be able to reduce this artifact would be much appreciated. If an alternative resin would alleviate this issue, please do let me know. Thanks in advance, Chaitanya Link to image of the block surface: https://cwru.box.com/s/5dsd7ngazlhf0jxga4apqh47a75uzb84 -------------- next part -------------- 1. Samples were extracted from cadavers and left in formalin for??more than a couple of month. 2. Cut 5 mm long nerve segments and rinse in PBS for 1h. Post-fix nerve in 2% osmium tetroxide for 2 hours. 3. Perform gradual dehydration in increasing steps of ethanol, about 2 steps per day for a total of 3 days (with rotation). 4. Pre-infiltrate specimen with a 1:1 mixture of 96% ethanol and 100% GMA solution for 2 days.??Perform vacuum cycles for about 30 min everyday with 35 psi in a vacuum pump. 5. Immerse sample in the infiltration mix (1g Hardener 1 in 100 ml GMA) for a total of 6 days, with 2 changes of fresh infiltration mix in between. Same vacuuming protocol as previous. 6. Polymerize with 15 parts infiltration mix and 1 part Hardener 2. 7. Polymerize in Teflon molds in vacuum at room temperature (25 deg C) for a couple of hours, followed by polymerization in the oven at 37 deg C. I see that the blocks are usually soft after this, so I let the samples continue to sit in the mold in the oven at 55 deg C for 48 hours. 8. Remove from mold with Technovit 3040, clamp on the motorized microtome (Microm) and section with tungsten carbide knife at 13 degrees clearance angle with a slow speed setting at 2-5 micron section thickness. From beth.oneil1 at wvumedicine.org Tue Jun 8 12:31:55 2021 From: beth.oneil1 at wvumedicine.org (O'Neil, Beth A.) Date: Tue, 8 Jun 2021 17:31:55 +0000 Subject: [Histonet] Problems with DIF staining of C4d Message-ID: We perform manual DIF staining at our facility and C4d has always been a problem. We end up repeating the stain more times than not due to lack of staining. We aliquot the FITC and freeze at -70C until ready for use. We also started doing the same with the C4d. Sometimes it works and sometimes it doesn't. My pathologist said it is not the FITC but the C4d itself. I have contacted the manufacturer of my C4d but they are very slow to respond. Any suggestions or experiences would be appreciated. Beth ONeil WVU Medicine, JW Ruby Memorial Hospital Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Timothy.Morken at ucsf.edu Tue Jun 8 12:48:47 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 8 Jun 2021 17:48:47 +0000 Subject: [Histonet] Problems with DIF staining of C4d In-Reply-To: References: Message-ID: Beth, which C4d are you using? And it is a FITC-labeled primary? We use it as a two-step rather than FITC-labeled primary. That increases the sensitivity significantly. Gloms have C4d so act as an internal control. We use C4d antibody, 100ul vial, unlabeled primary. Diluted 1:200 in Dako or Bond diluent. Quidel, Cat# A-213 Secondary Ab: Antibody, Goat anti Mouse IgG H+L FITC 1.5mg. Diluted 1:120 in Dako or Bond diluent. Jackson Immuno Research 115-095-062 We store the concentrate in the fridge. We use it daily so go through it pretty quickly, but we have it validated for two years at 2-8C. We make fresh dilutions a couple times per week. We stain on the Bond Autostainer, but manual will work fine as well. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: O'Neil, Beth A. via Histonet Sent: Tuesday, June 08, 2021 10:32 AM To: Histonet Subject: [Histonet] Problems with DIF staining of C4d We perform manual DIF staining at our facility and C4d has always been a problem. We end up repeating the stain more times than not due to lack of staining. We aliquot the FITC and freeze at -70C until ready for use. We also started doing the same with the C4d. Sometimes it works and sometimes it doesn't. My pathologist said it is not the FITC but the C4d itself. I have contacted the manufacturer of my C4d but they are very slow to respond. Any suggestions or experiences would be appreciated. Beth ONeil WVU Medicine, JW Ruby Memorial Hospital Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!4Xl35NDHqHTAYRldFRGqOf-tKFfRemSb2mAhe7ZD9pOA50Ae1cyajZI8a0ws-AKxODnSUA$ From relia1 at earthlink.net Tue Jun 8 13:07:22 2021 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 8 Jun 2021 14:07:22 -0400 Subject: [Histonet] How Can YOU Tell that Summer is HERE? Check out the exciting NEW Opportunities Nationwide with RELIA!! Message-ID: <000001d75c91$21e1d2e0$65a578a0$@earthlink.net> Hi Histopeeps, Summer is Here!!!! What tells you that Summer is here? ? That first bite of watermelon? ? That first sip of ice cold lemonade on a hot day? ? That first dip in the shimmering waters of a pool, lake or ocean? ? That Cookout, Baseball Game, State Fair, Fresh Corn? PROM, GRADUATION SUMMER VACATION!! I Love it all, except maybe the heat and bathing suit shopping (UGH!!) Another way I can tell that Summer is here is that my phone also starts ringing off the hook. Clients are calling from all over the country. It happens every year right after Memorial Day. I Have A Bunch Of New Opportunities To Tell You About and New Ones Coming In EVERY DAY!! Histopeeps, please take a look at my current openings. ? If something looks interesting let me know. ? If you know someone who might be interested please pass along the information. Remember if I place someone you refer to me you will earn a referral fee. If the opportunity for you is someplace else drop me an email to remind me where you want to be and what you want to do. I want YOU to be the person that pops in MY head when that client calls!!! Here are my Hottest!! Histology Opportunities: ? FL - Fort Myers Anatomic Pathology Manager Brand New Lab Facility!! ? FL - Fort Myers Assay Development Specialist ? IHC Guru!! ? TX ? Dallas IHC Tech Support Days! In house! ? CA - San Diego .Lead Tech ? Brand New Lab All shifts! ? CA ? Ventura Lead Histotech ? Days!! Run your own Lab!! ? CA - San Diego .Histotechs brand new lab all shifts!! ? CA - Aliso Viejo ...Histotechs all shifts!!! Opportunity for Advancement!! ? FL - Fort Myers ...Histotechs all shifts!! Opportunity for Advancement!! ? CA ? Modesto ... IHC Tech Nights ? State of the Art Lab! ? CA ? Modesto Histotech Nights ? State of the Art Lab! ? VA ? Norfolk .IHC Histology Tech ? Nights 10K Sign on Bonus!! ? TX ? Austin Histotech Nights ? Amazing team to work with! ? MD ? 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Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From igor.deyneko at gmail.com Tue Jun 8 22:51:13 2021 From: igor.deyneko at gmail.com (Igor) Date: Tue, 8 Jun 2021 23:51:13 -0400 Subject: [Histonet] Sample retention policy and tracking Message-ID: Hello fellow histonetters, I wanted to pick your minds with the following 2 questions pertaining to storing and trackingsamples in the research environment : 1. How do you track samples that you store long term? As far as slides and cassettes go, it makes sense that a lot of people use barcodes. Does anyone also store and track cell lines? How do you track them? 2. Do you have a sample retention policy for slides, cassettes, clinical samples and for cell lines?? If you do not mind sharing some details on how long you keep each kind of sample, I would greatly appreciate it! Thank you in advance! Igor Deyneko Scientific Operations Manager Novartis Institutes for Biomedical Research From carl.hobbs at kcl.ac.uk Wed Jun 9 08:48:51 2021 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Wed, 9 Jun 2021 13:48:51 +0000 Subject: [Histonet] Protocol for DAPI staining on paraffin sections Message-ID: Dear Alida my emails to your msu.montan.edu get returned to me as undeliverable I have incubated 2 dewaxed sections ( different slides) in HOECHST +/- HIER. Both stain nuclei very well. The HIER-treated section gave a brighter nuclear positivity ( so needed to lower exposure time on taking images) NSD though I can post on Histonet images, if you wish carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6810 From relia1 at earthlink.net Wed Jun 9 10:53:43 2021 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 9 Jun 2021 11:53:43 -0400 Subject: [Histonet] Histopeeps!! IHC Subject Matter Experts Needed in Florida, California, Texas and Virginia!! Excellent Opportunity to FLEX your IHC Muscles!!! Message-ID: <0b9a01d75d47$a10b4310$e321c930$@earthlink.net> Hi Histopeeps! I will make this short and sweet! RELIA HOT Histology Job Alert!! #histopeeps #ihc subject matter experts needed in #Florida #Texas #California & #Virginia Work in state of the art labs Excellent compensation including relo & sign on bonuses For more info contact Pam Barker DM/PM me Or email me at relia1 at earthlink.net Cell/Text 407-353-5070 #ilovemyhistopeeps #jobs4myhistopeeps Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From alida.bailleul at gmail.com Wed Jun 9 21:13:51 2021 From: alida.bailleul at gmail.com (Alida Bailleul) Date: Thu, 10 Jun 2021 10:13:51 +0800 Subject: [Histonet] Protocol for DAPI staining on paraffin sections In-Reply-To: References: Message-ID: Dear Carl, thank you so much for checking! Yes, may I please see some images? And would you mind to share your specific protocols and the brand also of the DAPI that you bought? (I am guessing it does not matter too much though). Thank you very very much, I really appreciate the help. All the best Alida ps: my sections are demineralized bone, it should work the same for any tissue right? Do you recommend to cut and then stain right away? Or would it also work on paraffin slides that were cut a few years ago? On Wed, Jun 9, 2021 at 10:01 PM Hobbs, Carl via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Dear Alida > my emails to your msu.montan.edu get returned to me as undeliverable > I have incubated 2 dewaxed sections ( different slides) in HOECHST +/- > HIER. > Both stain nuclei very well. > The HIER-treated section gave a brighter nuclear positivity ( so needed to > lower exposure time on taking images) > NSD though > I can post on Histonet images, if you wish > > carl > > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > > > 020 7848 6810 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Dr. Alida M. Bailleul Associate Research Fellow/Associate Professor Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences www.ivpp-avianevolution.com & Research Associate of Paleontology, Museum of the Rockies, Montana State University Google Scholar - ResearchGate From Taylor.Rosa at crl.com Thu Jun 10 06:30:51 2021 From: Taylor.Rosa at crl.com (Rosa, Taylor) Date: Thu, 10 Jun 2021 11:30:51 +0000 Subject: [Histonet] [External]: Cell Line Tracking Message-ID: Hello Igor, I used to work in a lab that tracked several hundred cell lines and I can tell you about the tracking system they used if you'd like. It was rudimentary but quite effective and the principles behind it could probably be updated/streamlined. Please feel free to send me an email at taylor.rosa at crl.com for more information. Thanks, Taylor C. Rosa, MS Associate Research Scientist Pathology Services | Charles River 4025 Stirrup Creek Drive, Suite 150, Durham, NC 27703 P: 919.206.7041 | F: 919.206.7001 taylor.rosa at crl.com | www.criver.com LinkedIn | Twitter | Facebook | Eureka -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: 09-Jun-2021 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [External]: Histonet Digest, Vol 211, Issue 9 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Ctaylor.rosa%40crl.com%7Cfd7d77f33a064ae4d2d608d92b682785%7C374f8930e1504031bb35483215fe5900%7C0%7C0%7C637588548610495286%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=%2Bda8%2F9hkJ1KoC8TMHL1au36wD0%2BVKQC1f6YaiX5RqE8%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From CDISBROW at msn.com Sun Jun 13 21:04:34 2021 From: CDISBROW at msn.com (Carrie Disbrow) Date: Mon, 14 Jun 2021 02:04:34 +0000 Subject: [Histonet] Unstained slides precut for IHC Message-ID: Hi! Current lab is cutting extra slides for IHC and putting in 60 degree oven for 45 mins to dry. If IHC is ordered a precut control and the already baked slides are again put in the 60 degree oven for 45 mins. Sometimes the control is cut and added to the dry slide or a separate slide. So, the questions have been should the slides be air dried first using a fan before baking and slides not baked until the pathologist places an order? Should the tissue and the control all have the same amount of time in the oven to ensure consistency? Also, is it better to rack slides standing or on edge for IHC? Additionally, when sending slides for IHC to other labs is it preferred to send dry slides or baked slides? Thanks for your input! Carrie Disbrow, HTL Get Outlook for Android From tony.henwood at health.nsw.gov.au Sun Jun 13 23:53:06 2021 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon, 14 Jun 2021 04:53:06 +0000 Subject: [Histonet] Unstained slides precut for IHC In-Reply-To: References: Message-ID: <1623646385966.11436@health.nsw.gov.au> Hi Carrie, Over-heating of sections (and tissue blocks) for IPX is probably one of the most significant and unappreciated pre-analytical factors that can affect immunolocalisation. We closely scrutinise slides closely for overheating, especially those sent to us for immunostaining. We strongly prefer air-dried sections that we place on our immunostaniners (we have both a Bond and Ventana Benchmark). The following might be useful: Controlled Section Baking for Immunohistochemistry One source of poor immunostaining is overheating of tissue and sections. Several authors have reported that heated slide drying adversely affects sensitivity in immunohistochemistry (1). Therefore, some have advocated the use of lower temperature drying using adhesive-coated slides to improve the sensitivity of the test (2-5). In one study (1), half the antigens were adversely affected by section drying at 80oC including 5D3, CMV, S100, HMB45 and CEA. Oates (4) used antisera to epithelial membrane antigen from three different companies and found that for slides dried at 58"C, staining was often paler than slides dried at room temperature or at 37?C. Low heat attachment of sections to slides can cause several issues including inadequate attachment of the tissue sections so that tissue sections may be lost during antigen recovery and/or immunostaining, the inability of some paraffins to melt well at 58oC, and the requirement of more than 1 hr before an immunohistochemical procedure may be started. It has been recommended that the most efficient protocol for mounting tissue sections to microscopic slides would be to attach the tissues overnight before applying the immunohistochemical procedure at a temperature at which all tissue mounting paraffins should melt (e.g., 65oC) (6). It should be remembered that a significant dewaxing of sections occurs when slides are heated a few degrees above the melting point of the wax. Laboratory ovens seem to be variable in their ability to maintain a constant temperature with the implication that it is possible to either over-cook sections thus adversely affecting antigens or under-heat them, possibly compromising subsequent de-waxing. There is also the human element. How often are slides removed from the oven at the required time? The modern automatic immunostainers have excellent on-board slide heating to achieve reproducible, accurate antigen retrieval. This feature also allows controlled ?baking? of sections and being able to programme a set time, removes the possibility of human error. At the Children?s Hospital, the Bond 3 is used for automated immunohistochemistry. A study was designed to assess the usefulness of on-board baking in routine immunohistochemistry. Control sections were immunostained for several antigens (see table) using the Bond 3 on-board baking and dewax facility. Freshly cut sections were dried at 37oC for 5 minutes to remove excess water. Slides were then loaded onto the Bond and the baking procedure used was 35 minutes at 63oC. Stained controls were compared with control slides stained prior to the instigation of the on-board bake procedure. The historic procedure involved heating sections at 63-65oC for 35 minutes in a large fan-forced dry-air oven (7). BCL-2 Mum-1 CD31 BCL-6 Calretinin SATB2 BOB-1 S100 CyclinD1 CD20 ALK-1 MPO CD21 Ki67 INI-1 CD3 Synaptophsin BRG-1 HMB-45 Chromogranin Inhibin Melan A Myogenin Desmin The results showed that there was no difference between controls stained with the historic compared to the on-board baking procedure except for BCL-6 which the new procedure gave stronger staining. (see figure). In conclusion, we expect that on-board baking of sections should allow laboratories to have better control over the pre-analytical variables that can adversely affect the immunohistochemistry staining. References 1. Henwood, A. F. (2005). Effect of slide drying at 80oC on immunohistochemistry. Journal of Histotechnology, 28(1), 45-46. 2. Wakins, J., Kellock, D., Gillet, C., Egan, M., Pontin, J. E., Millis, R. R., & Levinson, D. A. (1990). Enhancement of immunostaining. Histopathology, 17(2), 185-185. 3. Dodson, A., Davies, E., & Waring, J. (1991). APTES, a section adhesive for immunocytochemistry; and experiences of slide drying at room temperature. Histopathology, 19(5), 484-485. 4. Oates J. (1993) The effect of temperature on immunostaining. Br J Biomed Sci 50: 157-158, 5. Williams, J. H., Mepham, B. L., & Wright, D. H. (1997). Tissue preparation for immunocytochemistry. Journal of clinical pathology, 50(5), 422-428. 6. Jones, W. T., Stockard, C. R., & Grizzle, W. E. (2001). Effects of time and temperature during attachment of sections to microscope slides on immunohistochemical detection of antigens. Biotechnic & Histochemistry, 76(2), 55-58. 7. Henwood, A. F. (2012). The application of heated detergent dewaxing and rehydration to immunohistochemistry. Biotechnic & Histochemistry, 87(1), 46-50. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Carrie Disbrow via Histonet Sent: Monday, 14 June 2021 12:04 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Unstained slides precut for IHC Hi! Current lab is cutting extra slides for IHC and putting in 60 degree oven for 45 mins to dry. If IHC is ordered a precut control and the already baked slides are again put in the 60 degree oven for 45 mins. Sometimes the control is cut and added to the dry slide or a separate slide. So, the questions have been should the slides be air dried first using a fan before baking and slides not baked until the pathologist places an order? Should the tissue and the control all have the same amount of time in the oven to ensure consistency? Also, is it better to rack slides standing or on edge for IHC? Additionally, when sending slides for IHC to other labs is it preferred to send dry slides or baked slides? Thanks for your input! Carrie Disbrow, HTL Get Outlook for Android _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From ASelf at tidelandshealth.org Mon Jun 14 11:47:55 2021 From: ASelf at tidelandshealth.org (Amy Self) Date: Mon, 14 Jun 2021 16:47:55 +0000 Subject: [Histonet] Non-Gyn Cytology Competency Message-ID: Happy Monday and I hope that everyone had a great weekend ? Does anyone have a non-gyn and FNA cytology competency checklist that they would be willing to share with me? Thanks, Amy Self Senior Histology Technologist Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 Office: (843) 520-8711 aself at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From mward at wakehealth.edu Tue Jun 15 14:10:24 2021 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Tue, 15 Jun 2021 19:10:24 +0000 Subject: [Histonet] HSV1 and HSV2 Message-ID: We have always offered these two antibodies separately but recently we have begun having issues with our HSV2. In researching a new source I am seeing that some places do some sort of cocktail mixture of the two. What are others out there doing for this? Thanks in advance for your help. Martha Ward, Manager Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 From Richard.Cartun at hhchealth.org Tue Jun 15 14:26:01 2021 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 15 Jun 2021 19:26:01 +0000 Subject: [Histonet] HSV1 and HSV2 In-Reply-To: References: Message-ID: <8ec2667bfbf74174ba18691581be91ca@hhchealth.org> Hi Martha: We are using type-specific monoclonals from BioSB (Santa Barbara, CA). They also sell a cocktail of the two antibodies which is what we use for screening. Rarely, do we get requests for type-specific identification. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org -----Original Message----- From: Martha Ward-Pathology via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 15, 2021 3:10 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] HSV1 and HSV2 CAUTION: This email is from outside HHC. USE CARE when opening attachments or links. We have always offered these two antibodies separately but recently we have begun having issues with our HSV2. In researching a new source I am seeing that some places do some sort of cocktail mixture of the two. What are others out there doing for this? Thanks in advance for your help. Martha Ward, Manager Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!PM-BBy3bF7epr83ucPrDa0D21BtxagGkNt2mKWWY7SUaExNwbpL43czZRyw5F0A-LMQ$ Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From mward at wakehealth.edu Tue Jun 15 15:00:44 2021 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Tue, 15 Jun 2021 20:00:44 +0000 Subject: [Histonet] HSV1 and HSV2 In-Reply-To: <8ec2667bfbf74174ba18691581be91ca@hhchealth.org> References: <8ec2667bfbf74174ba18691581be91ca@hhchealth.org> Message-ID: Thank you for the information. I will look into your antibody source. Martha -----Original Message----- From: Cartun, Richard Sent: Tuesday, June 15, 2021 3:26 PM To: Martha Ward-Pathology Cc: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] RE: HSV1 and HSV2 Hi Martha: We are using type-specific monoclonals from BioSB (Santa Barbara, CA). They also sell a cocktail of the two antibodies which is what we use for screening. Rarely, do we get requests for type-specific identification. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org -----Original Message----- From: Martha Ward-Pathology via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 15, 2021 3:10 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] HSV1 and HSV2 CAUTION: This email is from outside HHC. USE CARE when opening attachments or links. We have always offered these two antibodies separately but recently we have begun having issues with our HSV2. In researching a new source I am seeing that some places do some sort of cocktail mixture of the two. What are others out there doing for this? Thanks in advance for your help. Martha Ward, Manager Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!PM-BBy3bF7epr83ucPrDa0D21BtxagGkNt2mKWWY7SUaExNwbpL43czZRyw5F0A-LMQ$ Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From relia1 at earthlink.net Wed Jun 16 09:37:58 2021 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 16 Jun 2021 10:37:58 -0400 Subject: [Histonet] You are gonna love this one: My coworker stole my spicy food, got sick and Blamed ME! Oh and some fantastic job opportunities that are RELIA EXCLUSIVES! Message-ID: <000001d762bd$34e123f0$9ea36bd0$@earthlink.net> Hello Histopeeps, I hope this will be an amazing week for you! Perhaps something in this email could make your week even more amazing. I have a thought provoking article to share and some incredible job opportunities. Here is that article that I found online! If you have time to read it I would love to know what you think! A coworker stole my spicy food, got sick and is blaming me! https://www.askamanager.org/2021/05/a-coworker-stole-my-spicy-food-got-sick- and-is-blaming-me-2.html Super- HOT RELIA Exclusive Spotlight Opportunities: Fort Myers, FL - IHC Subject Matter Specialist My client is in need of an IHC Guru to fill a role as their Assay Development Specialist. This is a full time permanent dayshift position and my client is offering a fantastic compensation package including relocation assistance and performance bonuses. San Diego, CA ? Histologists ? BRAND NEW CLINICAL LAB!! That?s right!! A brand new lab opening soon and I have been engaged to staff it. These will be full time permanent positions in a brand new state of the art lab. If you are interested contact me ASAP for the details!! Ft. Myers, FL Anatomic Pathology Manager This is an exciting opportunity and a newly created position for a brand new facility. Strong lab management and histology experience required. My client is offering a fantastic compensation package including relocation assistance and bonuses. Histopeeps!! I also have amazing job opportunities in: v California - Orange County and San Diego!! v California ? Just outside of San Francisco! v Florida - Beachside on the SW Coast! v Texas - Austin Funky and Fun! v N. Carolina ?Fayetteville small town charm and Ft. Bragg. v PA - Live and work at the foot of the mountains!! v Tennessee - Chattanooga and Nashville!! v Virginia - Histotech with IHC 10K Sign On bonus!! Most of these are RELIA Exclusives! That?s right you will only see them HERE! And New Opportunities are coming in on a daily basis!! All of these clients are offering full time permanent positions with excellent compensation packages including VERY competitive pay rates, fantastic benefits, relocation and/or sign on bonuses and a great team to work with!! I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. ? I can be reached toll free at 866-607-3542. 866-60RELIA ? Text or call me on my cell at 407-353-5070. ? Or email me at: relia1 at earthlink.net . Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From Jennifer.Titus at greenwichhospital.org Wed Jun 16 11:28:07 2021 From: Jennifer.Titus at greenwichhospital.org (Titus, Jennifer) Date: Wed, 16 Jun 2021 16:28:07 +0000 Subject: [Histonet] HSV control Message-ID: <7c1a9556de62447e84d67a1019123c16@greenwichhospital.org> Hi all, We ran out of good patient tissue controls for HSV IHC. We recently purchased slides from Statlab (not great), Mercedes medical (even less great) and Cellmarque (best ones). But they are pricey. Does anyone have extras, or want to set up a trade? Thank you, Jen Jennifer B Titus, PA (ASCP)CM Manager-Anatomic Pathology Greenwich Hospital 5 Perryridge Rd. Greenwich, CT 06830 Phone:203-863-3005 Jennifer.Titus at greenwichhospital.org www.greenwichhospital.org This message originates from the Yale New Haven Health System. The information contained in this message may be privileged and confidential. If you are the intended recipient you must maintain this message in a secure and confidential manner. If you are not the intended recipient, please notify the sender immediately and destroy this message. Thank you. From tkngflght at yahoo.com Mon Jun 21 19:14:10 2021 From: tkngflght at yahoo.com (Cheryl) Date: Tue, 22 Jun 2021 00:14:10 +0000 (UTC) Subject: [Histonet] startup lab validation materials - suggestions welcome! References: <1954162213.1932364.1624320850743.ref@mail.yahoo.com> Message-ID: <1954162213.1932364.1624320850743@mail.yahoo.com> Hi Guys - working with a little lab and we're starting from four walls and a floor.? It is a human tissue lab but we won't have access to human tissues until everything is set up and validated. I've read everything I can find- any thoughts on how to obtain tissues to test from processing through simple stains so we can get our program going?? It's all FFPE stuff. Hoping ya'll can help!! Cheryl Kerry, HT(ASCP) From jhill at vet.k-state.edu Tue Jun 22 10:59:26 2021 From: jhill at vet.k-state.edu (Jennifer Phinney) Date: Tue, 22 Jun 2021 15:59:26 +0000 Subject: [Histonet] IHC Research Technician Kansas State Veterinary Diagnostic Lab Message-ID: Hello HistoNetters, The Kansas State Veterinary Diagnostic Laboratory (KSVDL) within the College of Veterinary Medicine is seeking applicants for a Research Assistant. This position will have the primary responsibility of coordinating new incoming research projects and developing new tests related to immunohistochemistry. A large portion of this person's time will be spent developing new diagnostic and research assays along with coordinating research projects. This individual will also spend a decent amount of time performing diagnostic immunohistochemistry assays and will also assist in diagnostic routine histology duties related to service and research. KSVDL is an AAVLD accredited lab. If anyone is interested in applying, the link to the job posting may be found here: https://careers.k-state.edu/cw/en-us/job/510780/research-assistant-histology-veterinary-diagnostic-laboratory Jennifer Phinney MS, QIHC Histology Laboratory Administrator KSVDL From relia1 at earthlink.net Tue Jun 22 11:11:26 2021 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 22 Jun 2021 12:11:26 -0400 Subject: [Histonet] RELIA HOT Histology Job Alert 10-15K Bonuses - FL, CA, VA and MORE!!! Message-ID: <000501d76781$43645550$ca2cfff0$@earthlink.net> Hi Histopeeps, How are you? I hope you are having a great week like me! The phones haven?t stopped ringing Things are heating up just like the temps! So I thought I would shoot you a quick email and let you know about some new opportunities that came in late last week and over the weekend. I am really excited because these are great opportunities with stable companies experiencing growth, some are even RELIA EXCLIUSIVES!!! If you have vacations already planned my clients understand. You won?t have to change your plans if you change jobs. I PROMISE!!!!! So Histopeeps if you have a second grab a patch of shade and a cool beverage and take a second to browse the list of my current openings. I have histology leadership opportunities in: ? California ? Florida I have IHC Positions in: ? California ? Florida ? Virginia ? Texas I have histology technician/technologist opportunities in: ? Florida ? Texas ? Maryland ? Virginia ? California All of my clients offer excellent compensation, benefits. Relocation assistance and or sign on bonuses. (10-15K) All of these jobs are full time & permanent. I have opportunities available on ALL shifts! If you or anyone you know is interested in hearing more about any of these opportunities please contact me. If I place someone you refer to me you will earn a referral bonus!! I can be reached ASAP on my cell at 407-353-5070 or e-mail me at relia1 at earthlink.net or call me toll free at 866-607-3542. Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From c.tague at pathologyarts.com Tue Jun 22 11:14:18 2021 From: c.tague at pathologyarts.com (Curt Tague) Date: Tue, 22 Jun 2021 16:14:18 +0000 Subject: [Histonet] Specimen tracking scenario, accountability Message-ID: So we're in a reference lab setting here, it's a little different than the hospital environment... I've been in both. We receive specimens from a variety of different hospitals, physician offices and even other labs for histology (TC), we send the slides back. What I'd like to do is implement some system for tracking blocks throughout the process but with so many different clients and number systems/prefixes, most of which are NOT barcoded, it can be a challenge tracking the blocks throughout the whole process... from receipt to embedding to microtomy... Aside from manually documenting every blocks on a paper log sheet, would anyone have any suggestions on how to track these things? My initial thought is a simple digital photo of the basket at embedding (when removed from the processor) then another photo of blocks you take to your microtome and perhaps one last photo when blocks are taken from your cutting station to be filed or returned... the simple issue is tracking who is in possession of what blocks at all times... accountability... but this seems to present challenges too... Any thoughts are appreciated. Curt From Jennifer.Wooten at tricore.org Tue Jun 22 13:11:49 2021 From: Jennifer.Wooten at tricore.org (Wooten, Jennifer) Date: Tue, 22 Jun 2021 18:11:49 +0000 Subject: [Histonet] Specimen tracking scenario, accountability In-Reply-To: References: Message-ID: Hi Curt, That sounds like a very difficult position to be in, especially with many of them not barcoded. Photo documentation might be your best bet; we use the Vantage tracking system throughout our process, and the Arcos system for tracking filed blocks once completed. You might want to reach out to Epredia to see if their Arcos system allows for scanning of trays without barcodes. Good luck! Jennifer Wooten, BA, BS, HTL (ASCP)CM Pronouns: she/her/hers Technical Supervisor | Anatomic Pathology | University Hospital Jennifer.Wooten at tricore.org Desk: 505.272.5486 | Fax: 505.272.0240 TriCore Reference Laboratories 2211 Lomas Blvd NE Albuquerque, NM 87106 www.tricore.org -----Original Message----- From: Curt Tague Sent: Tuesday, June 22, 2021 10:14 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Specimen tracking scenario, accountability So we're in a reference lab setting here, it's a little different than the hospital environment... I've been in both. We receive specimens from a variety of different hospitals, physician offices and even other labs for histology (TC), we send the slides back. What I'd like to do is implement some system for tracking blocks throughout the process but with so many different clients and number systems/prefixes, most of which are NOT barcoded, it can be a challenge tracking the blocks throughout the whole process... from receipt to embedding to microtomy... Aside from manually documenting every blocks on a paper log sheet, would anyone have any suggestions on how to track these things? My initial thought is a simple digital photo of the basket at embedding (when removed from the processor) then another photo of blocks you take to your microtome and perhaps one last photo when blocks are taken from your cutting station to be filed or returned... the simple issue is tracking who is in possession of what blocks at all times... accountability... but this seems to present challenges too... Any thoughts are appreciated. Curt IMPORTANT: This message originates from TriCore Reference Laboratories or one of its affiliate organizations or representatives. Please note that the information contained in this e-mail message (including any attachments) should be considered confidential and intended for the use of the individual or entity named appropriately. If the reader of this message is any other than the individual named above or an agent responsible for delivery, you are hereby notified that any inappropriate dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately. Any errant copy of this message must be deleted. From Timothy.Morken at ucsf.edu Tue Jun 22 17:55:27 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 22 Jun 2021 22:55:27 +0000 Subject: [Histonet] Specimen tracking scenario, accountability In-Reply-To: References: Message-ID: Curt, You can make and use barcodes in MS Excel or Access and print labels. If you keep the clients items together in some kind of bag or container you can put the label on the outside and scan at each station to track them. You just need to be sure the items are all kept together to ensure nothing gets lost. A photo now and then at critical steps could be helpful (we still take pics of all racks going into our processors - trying to scan cassette barcodes in racks is difficult). https://www.smartsheet.com/content/excel-barcodes Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Curt Tague via Histonet Sent: Tuesday, June 22, 2021 9:14 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Specimen tracking scenario, accountability So we're in a reference lab setting here, it's a little different than the hospital environment... I've been in both. We receive specimens from a variety of different hospitals, physician offices and even other labs for histology (TC), we send the slides back. What I'd like to do is implement some system for tracking blocks throughout the process but with so many different clients and number systems/prefixes, most of which are NOT barcoded, it can be a challenge tracking the blocks throughout the whole process... from receipt to embedding to microtomy... Aside from manually documenting every blocks on a paper log sheet, would anyone have any suggestions on how to track these things? My initial thought is a simple digital photo of the basket at embedding (when removed from the processor) then another photo of blocks you take to your microtome and perhaps one last photo when blocks are taken from your cutting station to be filed or returned... the simple issue is tracking who is in possession of what blocks at all times... accountability... but this seems to present challenges too... Any thoughts are appreciated. Curt _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LQC6Cpwp!-M9oifTHePCDuG19yly7OSM-sno8gl8BZo2G6ibBbZnIAo18tESgLPHhT3avdf_k7IiVkw$ From relia1 at earthlink.net Wed Jun 23 10:47:45 2021 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 23 Jun 2021 11:47:45 -0400 Subject: [Histonet] RELIA Histology Special Careers Bulletin Leadership opportunities with bonuses up to 10K and more!! Message-ID: <000001d76847$1cc1ab20$56450160$@earthlink.net> Hello Histopeeps, How are you? I hope you are having a wonderful day. I have a couple of histology leadership positions and I need your help. I am currently working with clients in California and Florida* that are in need of exceptional histology professionals for a variety of leadership roles. My clients are offering excellent compensation, exciting work and fantastic relocation and bonus packages!! In Florida I am recruiting for: ? Anatomic Pathology Manager ? Newly created position in SW FL! ? Assay Development Specialist ? Top Notch IHC Guru in SW FL In California I am recruiting for: ? Dayshift Histology Supervisor ? Brand NEW LAB in San Diego ? Night Shift Supervisor ? Brand NEW LAB in San Diego ? Histology Supervisor/lead tech ? Run your own Lab in Ventura! In Texas I am recruiting for: ? Technical Support Specialist ? Days Full time Dallas! My question is do you know of anyone who might be interested in management positions in any of these areas? Or Histopeeps, Would you be interested in an opportunity in one of these areas? Incidentally, some of my clients are willing to consider lead techs ready to step up to supervisor! *The Florida Supervisor License is required for The Florida position but it is easy to get!!* I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542, on my cell at 407-353-5070 or relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From relia1 at earthlink.net Thu Jun 24 11:00:47 2021 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 24 Jun 2021 12:00:47 -0400 Subject: [Histonet] Histopeeps! Having trouble with open positions? I CAN help! Message-ID: <009201d76912$19f84db0$4de8e910$@earthlink.net> Hi Histopeeps! Lots of histology jobs out there! I can help!!! Put me in touch with your hiring manager and let me see what I can do to help you get an amazing histopeep to join your team. Here's my info: Pam Barker Phone: (407)657-2027 Cell: (407)353-5070 E-mail: relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From craigak12 at gmail.com Mon Jun 28 13:11:47 2021 From: craigak12 at gmail.com (J B) Date: Mon, 28 Jun 2021 11:11:47 -0700 Subject: [Histonet] Tech time/Staffing calculations Message-ID: Hello, Does anyone have any information on how they calculate staff for HT's, Lab Assistants, and transcription/send outs? Do you have a basic calculation for block count or case count? If so, can you share it with me? Anything is greatly appreciated. We are a small AP lab performing IHC, SS, etc. Thank you, Julie From pslath at gwu.edu Wed Jun 30 15:31:50 2021 From: pslath at gwu.edu (Patricia Latham) Date: Wed, 30 Jun 2021 16:31:50 -0400 Subject: [Histonet] Bone decalcification Message-ID: To Histopeeps, Does anyone know if there is a method to decalcify bone once it is FFPE? Thank you, Pat L George Washington University Washington, DC From llewllew at shaw.ca Wed Jun 30 17:12:31 2021 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Wed, 30 Jun 2021 15:12:31 -0700 Subject: [Histonet] Bone decalcification In-Reply-To: References: Message-ID: <99ab5b90-43f4-91b0-36e2-0c3f5f0375a3@shaw.ca> Use surface decalcification: Surface the block, then remove from the microtome. Do not use the microtome for another block, so as to avoid adjusting the position of the block face to the knife. Place the block face down in 4% nitric acid for 2 hours or longer. Rinse the block and cool down. Place back into the microtome. Use a fresh blade to ensure sharpness. You should be able to get 3 or 4 sections at 5 microns before hitting the calcium again. Bryan Llewellyn Patricia Latham via Histonet wrote: > To Histopeeps, > Does anyone know if there is a method to decalcify bone once it is FFPE? > Thank you, > Pat L > George Washington University > Washington, DC > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From umbellas at hotmail.com Wed Jun 30 21:36:21 2021 From: umbellas at hotmail.com (hotmail) Date: Thu, 1 Jul 2021 10:36:21 +0800 Subject: [Histonet] Bone decalcification In-Reply-To: References: Message-ID: In my experience soaking the block in water works just as well as surface decal. If you need to you can take the bone back, by reverse processing. Run the block through the clean cycle back to water, then leave the bone in NBF for 24-48hrs. The fixative will protect the tissue when you put it back into decal. Gerard Spoelstra Sent from my iPad > On 1 Jul 2021, at 4:37 am, Patricia Latham via Histonet wrote: > > ?To Histopeeps, > Does anyone know if there is a method to decalcify bone once it is FFPE? > Thank you, > Pat L > George Washington University > Washington, DC > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet