[Histonet] frozen section problem

[John Kiernan]

Yes, definitely ice crystal holes! If the tissues are unfixed you will have to freeze much more rapidly (isopentane cooled with liquid nitrogen.) If fixed in formaldehyde, cryoprotect by immersing the pieces in 20% sucrose, until they sink.

John Kiernan
Anatomy & Cell Biology, UWO
London, Canada
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________________________________
From: Bonello Dorianne M at Health-MDH via Histonet <histonet at lists.utsouthwestern.edu>
Sent: July 16, 2021 11:25 AM
To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] frozen section problem

Dear all,


We are experiencing freezing artifacts on our frozen sections. Basically, we are seeing cavity-like structures under the microscope, mostly elongated, especially when it's a frozen section on brain tissue. This is most probably happening due to ice crystal formation. We're not using cryospray, relying only on the cryobar boost function.


Does anyone has a solution to this problem please?


Regards,



Dorianne Bonello
Allied Health Practitioner (MLS)
Histology Laboratory - Pathology
Health-Mater Dei Hospital


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T +356 +356 25456434

E dorianne.m.bonello at gov.mt


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