From Eric_Gilchrist at unc.edu Tue Jul 6 08:19:15 2021 From: Eric_Gilchrist at unc.edu (Gilchrist, Eric P) Date: Tue, 6 Jul 2021 13:19:15 +0000 Subject: [Histonet] Sectioning problems with Fisher Replacement Paraffin Message-ID: Good Morning, When Fisher stopped producing The TissuePrep 2 Paraffin formulation last year, we were told by the Fisher rep that the new "Histoplast PE" was an equivalent replacement for the TissuePrep 2. We have had nothing but irritation with this formulation with a magnitude increase in wrinkles and folds. In short, it is NOT an equivalent product. Does anyone have any recommendations? Is the "Histoplast LP" any better? Thanks in advance for your responses. Eric Gilchrist, Supervisor UNC Oral and Maxillofacial Pathology Laboratory This communication may contain information that is confidential, privileged or otherwise protected from use or disclosure under state and federal law. It is intended only for the person to whom it is addressed and the information should be kept confidential and secure. IF YOU HAVE RECEIVED THIS COMMUNICATION IN ERROR, PLEASE NOTIFY THE SENDER IMMEDIATELY, then destroy the entire communication and any attachments. Thank you for your cooperation. From Jennifer.Wooten at tricore.org Tue Jul 6 10:22:56 2021 From: Jennifer.Wooten at tricore.org (Wooten, Jennifer) Date: Tue, 6 Jul 2021 15:22:56 +0000 Subject: [Histonet] Histonet Digest, Vol 212, Issue 1 In-Reply-To: References: Message-ID: We surface decal with decal I for about 20-30 minutes (as long as you don't need to do any molecular studies). You can usually get 3-4 good sections before its crunchy again. Jennifer Wooten, BA, BS, HTL (ASCP)CM Pronouns: she/her/hers Technical Supervisor | Anatomic Pathology | University Hospital Jennifer.Wooten at tricore.org Desk: 505.272.5486 | Fax: 505.272.0240 TriCore Reference Laboratories 2211 Lomas Blvd NE Albuquerque, NM 87106 www.tricore.org -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Thursday, July 1, 2021 11:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 212, Issue 1 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!NYT1fPLx!rZGhPQFpkcDFHp1RyxjoEoWM4WM8t9SIjI6-QlfNFzm9oMImyxhM3BlAiaAfDKxDMPB-BQ$ or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." IMPORTANT: This message originates from TriCore Reference Laboratories or one of its affiliate organizations or representatives. Please note that the information contained in this e-mail message (including any attachments) should be considered confidential and intended for the use of the individual or entity named appropriately. If the reader of this message is any other than the individual named above or an agent responsible for delivery, you are hereby notified that any inappropriate dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately. Any errant copy of this message must be deleted. From Charles.Bacon at baystatehealth.org Tue Jul 6 13:22:25 2021 From: Charles.Bacon at baystatehealth.org (Bacon, Charles) Date: Tue, 6 Jul 2021 18:22:25 +0000 Subject: [Histonet] Sectioning problems with Fisher Replacement Paraffin In-Reply-To: References: Message-ID: <3e7629c88f5f4db58525693cdec04073@ZXSWEXCHMXPR06.bhs.org> Hi Eric, We use a great product from Polyscientific, called "Infiltrating/Embedding Paraffin Prills" Item Number: c827 https://www.polyrnd.com/product/infiltratingembedding-paraffin-prills/. I am in no way affiliated with this company so this is just a simple Histotech review. As the name suggests, it can actually be used for infiltration in processing and for embedding/microtomy. I have used it at two different medical centers here in the Western Massachusetts area with great luck. I will tell you that we have Sakura processors and embedding centers. We use Leica RM2255 microtomes, and we do keep our blocks on ice while cutting. I hope this info helps. Good luck, Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786? Fax: 413-322-4790 Charles.Bacon at baystatehealth.org -----Original Message----- From: Gilchrist, Eric P [mailto:Eric_Gilchrist at unc.edu] Sent: Tuesday, July 06, 2021 9:19 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Sectioning problems with Fisher Replacement Paraffin Good Morning, When Fisher stopped producing The TissuePrep 2 Paraffin formulation last year, we were told by the Fisher rep that the new "Histoplast PE" was an equivalent replacement for the TissuePrep 2. We have had nothing but irritation with this formulation with a magnitude increase in wrinkles and folds. In short, it is NOT an equivalent product. Does anyone have any recommendations? Is the "Histoplast LP" any better? Thanks in advance for your responses. Eric Gilchrist, Supervisor UNC Oral and Maxillofacial Pathology Laboratory This communication may contain information that is confidential, privileged or otherwise protected from use or disclosure under state and federal law. It is intended only for the person to whom it is addressed and the information should be kept confidential and secure. IF YOU HAVE RECEIVED THIS COMMUNICATION IN ERROR, PLEASE NOTIFY THE SENDER IMMEDIATELY, then destroy the entire communication and any attachments. Thank you for your cooperation. ---------------------------------------------------------------------- Please view our annual report at http://www.bhannualreport.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at 413-794-0000 and destroy all copies of this communication and any attachments. For further information regarding Baystate Health's privacy policy, please visit our Internet site at https://www.baystatehealth.org. From plucas at biopath.org Wed Jul 7 12:47:59 2021 From: plucas at biopath.org (Paula) Date: Wed, 7 Jul 2021 10:47:59 -0700 Subject: [Histonet] "cooked" biopsy Message-ID: <000001d77358$3ad2aa80$b077ff80$@biopath.org> Hello, good day, Our pathologist is complaining about the tissues today that they are "cooked, burnt, crushed, shrunken" those are the adjectives she is using. Can you tell me the cause? Usually, the work comes out beautiful but today they are not. Nothing has changed on our processing times. What should I investigate? Thank you in advance, Paula Bio-Path Medica Group From plucas at biopath.org Wed Jul 7 13:53:01 2021 From: plucas at biopath.org (Paula) Date: Wed, 7 Jul 2021 11:53:01 -0700 Subject: [Histonet] "cooked" biopsy In-Reply-To: <0FD39A81-2536-4909-9729-51150C9E1FD6@icloud.com> References: <000001d77358$3ad2aa80$b077ff80$@biopath.org> <0FD39A81-2536-4909-9729-51150C9E1FD6@icloud.com> Message-ID: <001d01d77361$50ae2150$f20a63f0$@biopath.org> Thank you, everyone... I looked at my reagents and saw the color pink in the xylene, which tells me that there is water or too much water in it so I changed it. We recycle xylene, so I need to get the recycler looked at now. Thanks again, Paula -----Original Message----- From: Erick Rodriguez [mailto:rodriguez.erick at icloud.com] Sent: Wednesday, July 07, 2021 11:24 AM To: Paula Subject: Re: [Histonet] "cooked" biopsy Did you change the processor reagents before running your tissues? Cooked tissue usually means the tissue wasn?t dehydrated properly and the leftover water boiled and fried your tissue. I would double check the alcohols. > On Jul 7, 2021, at 11:00 AM, Paula via Histonet wrote: > > ?Hello, good day, > > > > Our pathologist is complaining about the tissues today that they are > "cooked, burnt, crushed, shrunken" those are the adjectives she is using. > > > > Can you tell me the cause? Usually, the work comes out beautiful but today > they are not. Nothing has changed on our processing times. > > > > What should I investigate? > > > > Thank you in advance, > > Paula > > Bio-Path Medica Group > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Carole.Johnson at uchealth.org Wed Jul 7 14:14:37 2021 From: Carole.Johnson at uchealth.org (Johnson, Carole) Date: Wed, 7 Jul 2021 19:14:37 +0000 Subject: [Histonet] Iron stain on Bone Marrow crush specimens Message-ID: Hello, Would anyone be willing to share their protocol for performing iron stains on bone marrow crush specimens? We are trying to convert from doing the stain manually to automated on a Ventana Special Stainer using the iron stain kit from Roche. We are using the following protocol: 1. Fix slide in methanol x 2 minutes 2. Allow slide to air dry 3. Rinse slide in distilled water 4. Place slide on special stainer (Ventana only allows adjusting counterstain time, currently set at 4 minutes) My plan is to pull additional crush smears and trying different counterstain times, but if anyone has other suggestions, it would be greatly appreciated. Best Regards, Carole Carole L Johnson, HT, QIHC (ASCP) CONFIDENTIALITY NOTICE: This message and any attachments may contain privileged and confidential peer review, risk management, and/or quality management information pursuant to the Colorado Professional Review Act, C.R.S. 12-36.5-101, et seq., the Colorado Hospital Licensing law, C.R.S. 25-3-109, the Quality Management Programs law, C.R.S. 25-35-904, and other corresponding provisions of federal and state law. Please maintain the strict confidentiality of this information. If you are not the intended recipient, you are notified that any disclosure, copying, distribution, electronic storage or use of this communication is prohibited. If you received this communication in error, please notify us immediately by e-mail, attaching the original message, and delete the original message from your computer and any network to which your computer is connected. From olivia at ka-recruiting.com Wed Jul 7 14:34:42 2021 From: olivia at ka-recruiting.com (Olivia Sloane) Date: Wed, 7 Jul 2021 15:34:42 -0400 Subject: [Histonet] Histotechnician/Grosser Needed Long Island NY Message-ID: Good Afternoon- New permanent histology opening! *Histotechnician/Grosser Needed Long Island, NY* - Permanent full time position - Shift is Tuesday to Saturday 6:00am - 2:30pm but seems to have flexibility - Candidates must have New York State Clinical Laboratory Technologist/Technician or Histological Technician License - Ideally would like to see candidates with 1+ year of experience - In this role you will embed, cut, stain and coverslip specimens. Set up specimens and their paperwork for grossing. Maintain the tissue processor, embedding centers, microtomes, stainers and coverslipper, etc. - Full benefits included and much more!!! - Position is located on Western Long Island- only about 35 miles from Manhattan. If you would like to talk further about these openings I am working on please send an updated resume to olivia at ka-recruiting.com with the best time and phone number for me to reach you! Or feel free to reach out to my direct line which is 617-746-2743 . You can also schedule an appointment with my calendar: https://calendly.com/olivia-ka/15min *Other Permanent Histology Openings Nationwide* FL - Tampa Area- Histology Manager GA ? 1.5 Hours South of Atlanta - Histotech (3 am - 11 am) NC ? Winston-Salem Area - Histotech NY- Bronx Area- Histotech NY - Buffalo Area- CLT Histology Lab Pathology NY - Westchester - Histotech NY - Westchester - IHC Lab Tech (Histotech) NY - Westchester - IHC Lead Tech (Histotech) NY ? Syracuse Area - Histotech NY ? Long Island - Histotechnician or Histotechnologist NY - Rochester Area- Histotechnologist (Day shift) OR - Northwest- Grossing Histotech OR - Northwest - Histotech PA - Southeast - Histotechnologist PA - Southeast - Histotech TN - Nashville Area- Histotech VA - Southeast - Senior Histotech VA - Southeast - Histotech VA ? Roanoke Area - Histotech WI ? Greater Green Bay Area - Histotech WI ? Greater Green Bay Area - Lab Histotech Trainer Talk to you soon, Olivia Sloane Healthcare Recruiter, K.A. Recruiting, Inc. * 617-746-2743 * olivia at ka-recruiting.com www.ka-recruiting.com From tony.henwood at health.nsw.gov.au Wed Jul 7 15:24:33 2021 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 7 Jul 2021 20:24:33 +0000 Subject: [Histonet] "cooked" biopsy In-Reply-To: <001d01d77361$50ae2150$f20a63f0$@biopath.org> References: <000001d77358$3ad2aa80$b077ff80$@biopath.org> <0FD39A81-2536-4909-9729-51150C9E1FD6@icloud.com>, <001d01d77361$50ae2150$f20a63f0$@biopath.org> Message-ID: <1625689472577.81801@health.nsw.gov.au> Hi Paula, We can check the purity of the xylene quite easily: Xylene Purity Test Procedure Note: The recommended and most accurate method of determining the purity of the recycled xylene is by doing a Gas Chromatography analysis. The following method can be used to obtain an acceptable confidence level in the purity of the recycled xylene (CBG Biotech). Testing Procedure 1. To a clean, dry 100 ml mixing cylinder graduate, add sufficient recovered xylene so that the bottom of the meniscus is aligned with the top edge of the 85 ml mark on the graduate. 2. Add water to the graduate until the bottom of the meniscus aligns with the top edge of the 100 ml mark on the graduate. At this point, 15 ml of water will have been added to 85 ml of recovered xylene. 3. Stopper the graduate and invert the mixture. Allow the mixture to settle, making sure that all of the water settles to the bottom of the graduate. No water should remain clinging to the sides of the graduate above the xylene/water separation point. This separation point should be near the 15 ml level of the graduate. (Note: xylene floats on top of the water). 4. Carefully inspect and record the point of separation between the water and xylene using the bottom of the meniscus as the separation point. 5. Subtract 15 ml from the quantity of water indicated in step 5. The remainder plus an additional 0.1 correction factor equals the percentage of recovered xylene impurities. EXAMPLE: Xylene/Water separation point is indicated to be 15.5 ml. (15.5 - 15) + 0.1 = 0.6% impurities. Therefore, the recovered xylene is 99.4% pure. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Paula via Histonet Sent: Thursday, 8 July 2021 04:53 To: 'Erick Rodriguez' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] "cooked" biopsy Thank you, everyone... I looked at my reagents and saw the color pink in the xylene, which tells me that there is water or too much water in it so I changed it. We recycle xylene, so I need to get the recycler looked at now. Thanks again, Paula -----Original Message----- From: Erick Rodriguez [mailto:rodriguez.erick at icloud.com] Sent: Wednesday, July 07, 2021 11:24 AM To: Paula Subject: Re: [Histonet] "cooked" biopsy Did you change the processor reagents before running your tissues? Cooked tissue usually means the tissue wasn?t dehydrated properly and the leftover water boiled and fried your tissue. I would double check the alcohols. > On Jul 7, 2021, at 11:00 AM, Paula via Histonet wrote: > > ?Hello, good day, > > > > Our pathologist is complaining about the tissues today that they are > "cooked, burnt, crushed, shrunken" those are the adjectives she is using. > > > > Can you tell me the cause? Usually, the work comes out beautiful but today > they are not. Nothing has changed on our processing times. > > > > What should I investigate? > > > > Thank you in advance, > > Paula > > Bio-Path Medica Group > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From plucas at biopath.org Thu Jul 8 07:59:12 2021 From: plucas at biopath.org (Paula) Date: Thu, 8 Jul 2021 05:59:12 -0700 Subject: [Histonet] "cooked" biopsy In-Reply-To: <1625689472577.81801@health.nsw.gov.au> References: <000001d77358$3ad2aa80$b077ff80$@biopath.org> <0FD39A81-2536-4909-9729-51150C9E1FD6@icloud.com>, <001d01d77361$50ae2150$f20a63f0$@biopath.org> <1625689472577.81801@health.nsw.gov.au> Message-ID: <002101d773f9$0e5c7430$2b155c90$@biopath.org> Hi Tony, Thank you for the procedure. I do send out the recycled xylene to have its analysis done by an outside company every month, and I'll put into place this testing procedure that you outlined below in place ase well. Paula -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood at health.nsw.gov.au] Sent: Wednesday, July 07, 2021 1:25 PM To: 'Erick Rodriguez'; Paula Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] "cooked" biopsy Hi Paula, We can check the purity of the xylene quite easily: Xylene Purity Test Procedure Note: The recommended and most accurate method of determining the purity of the recycled xylene is by doing a Gas Chromatography analysis. The following method can be used to obtain an acceptable confidence level in the purity of the recycled xylene (CBG Biotech). Testing Procedure 1. To a clean, dry 100 ml mixing cylinder graduate, add sufficient recovered xylene so that the bottom of the meniscus is aligned with the top edge of the 85 ml mark on the graduate. 2. Add water to the graduate until the bottom of the meniscus aligns with the top edge of the 100 ml mark on the graduate. At this point, 15 ml of water will have been added to 85 ml of recovered xylene. 3. Stopper the graduate and invert the mixture. Allow the mixture to settle, making sure that all of the water settles to the bottom of the graduate. No water should remain clinging to the sides of the graduate above the xylene/water separation point. This separation point should be near the 15 ml level of the graduate. (Note: xylene floats on top of the water). 4. Carefully inspect and record the point of separation between the water and xylene using the bottom of the meniscus as the separation point. 5. Subtract 15 ml from the quantity of water indicated in step 5. The remainder plus an additional 0.1 correction factor equals the percentage of recovered xylene impurities. EXAMPLE: Xylene/Water separation point is indicated to be 15.5 ml. (15.5 - 15) + 0.1 = 0.6% impurities. Therefore, the recovered xylene is 99.4% pure. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Paula via Histonet Sent: Thursday, 8 July 2021 04:53 To: 'Erick Rodriguez' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] "cooked" biopsy Thank you, everyone... I looked at my reagents and saw the color pink in the xylene, which tells me that there is water or too much water in it so I changed it. We recycle xylene, so I need to get the recycler looked at now. Thanks again, Paula -----Original Message----- From: Erick Rodriguez [mailto:rodriguez.erick at icloud.com] Sent: Wednesday, July 07, 2021 11:24 AM To: Paula Subject: Re: [Histonet] "cooked" biopsy Did you change the processor reagents before running your tissues? Cooked tissue usually means the tissue wasn?t dehydrated properly and the leftover water boiled and fried your tissue. I would double check the alcohols. > On Jul 7, 2021, at 11:00 AM, Paula via Histonet wrote: > > ?Hello, good day, > > > > Our pathologist is complaining about the tissues today that they are > "cooked, burnt, crushed, shrunken" those are the adjectives she is using. > > > > Can you tell me the cause? Usually, the work comes out beautiful but today > they are not. Nothing has changed on our processing times. > > > > What should I investigate? > > > > Thank you in advance, > > Paula > > Bio-Path Medica Group > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From john.garratt at ciqc.ca Fri Jul 9 14:31:01 2021 From: john.garratt at ciqc.ca (John Garratt) Date: Fri, 09 Jul 2021 19:31:01 +0000 Subject: [Histonet] "cooked" biopsy In-Reply-To: <002101d773f9$0e5c7430$2b155c90$@biopath.org> References: <000001d77358$3ad2aa80$b077ff80$@biopath.org> <0FD39A81-2536-4909-9729-51150C9E1FD6@icloud.com> <001d01d77361$50ae2150$f20a63f0$@biopath.org> <1625689472577.81801@health.nsw.gov.au> <002101d773f9$0e5c7430$2b155c90$@biopath.org> Message-ID: Interesting discussion. At CPQA we recently started a H&E EQA program that includes fixation and processing in the feedback to participants and in a recent mini-survey I found that a third of labs use recycled product somewhere in the pre-analytic phase. When we are continuously challenged by pre-analytic variables in histotechnology why do labs continue to use recycled reagents? John On Thu, Jul 8, 2021 at 5:59 AM, Paula via Histonet wrote: > Hi Tony, > Thank you for the procedure. I do send out the recycled xylene to have its analysis done by an outside company every month, and I'll put into place this testing procedure that you outlined below in place ase well. > Paula > > -----Original Message----- > From: Tony Henwood (SCHN) [mailto:tony.henwood at health.nsw.gov.au] > Sent: Wednesday, July 07, 2021 1:25 PM > To: 'Erick Rodriguez'; Paula > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] "cooked" biopsy > > Hi Paula, > > We can check the purity of the xylene quite easily: > > Xylene Purity Test Procedure > > Note: The recommended and most accurate method of determining the purity of the recycled xylene is by doing a Gas Chromatography analysis. The following method can be used to obtain an acceptable confidence level in the purity of the recycled xylene (CBG Biotech). > > Testing Procedure > > 1. To a clean, dry 100 ml mixing cylinder graduate, add sufficient recovered xylene so that the bottom of the meniscus is aligned with the top edge of the 85 ml mark on the graduate. > > 2. Add water to the graduate until the bottom of the meniscus aligns with the top edge of the 100 ml mark on the graduate. At this point, 15 ml of water will have been added to 85 ml of recovered xylene. > > 3. Stopper the graduate and invert the mixture. Allow the mixture to settle, making sure that all of the water settles to the bottom of the graduate. No water should remain clinging to the sides of the graduate above the xylene/water separation point. This separation point should be near the 15 ml level of the graduate. (Note: xylene floats on top of the water). > > 4. Carefully inspect and record the point of separation between the water and xylene using the bottom of the meniscus as the separation point. > > 5. Subtract 15 ml from the quantity of water indicated in step 5. The remainder plus an additional 0.1 correction factor equals the percentage of recovered xylene impurities. > > EXAMPLE: > > Xylene/Water separation point is indicated to be 15.5 ml. > (15.5 - 15) + 0.1 = 0.6% impurities. > Therefore, the recovered xylene is 99.4% pure. > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children?s Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > ________________________________________ > From: Paula via Histonet > Sent: Thursday, 8 July 2021 04:53 > To: 'Erick Rodriguez' > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] "cooked" biopsy > > Thank you, everyone... > I looked at my reagents and saw the color pink in the xylene, which tells me that there is water or too much water in it so I changed it. > We recycle xylene, so I need to get the recycler looked at now. > Thanks again, > Paula > > -----Original Message----- > From: Erick Rodriguez [mailto:rodriguez.erick at icloud.com] > Sent: Wednesday, July 07, 2021 11:24 AM > To: Paula > Subject: Re: [Histonet] "cooked" biopsy > > Did you change the processor reagents before running your tissues? Cooked tissue usually means the tissue wasn?t dehydrated properly and the leftover water boiled and fried your tissue. I would double check the alcohols. > >> On Jul 7, 2021, at 11:00 AM, Paula via Histonet wrote: >> >> ?Hello, good day, >> >> >> >> Our pathologist is complaining about the tissues today that they are >> "cooked, burnt, crushed, shrunken" those are the adjectives she is using. >> >> >> >> Can you tell me the cause? Usually, the work comes out beautiful but today >> they are not. Nothing has changed on our processing times. >> >> >> >> What should I investigate? >> >> >> >> Thank you in advance, >> >> Paula >> >> Bio-Path Medica Group >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Fri Jul 9 20:07:53 2021 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sat, 10 Jul 2021 01:07:53 +0000 Subject: [Histonet] "cooked" biopsy In-Reply-To: References: <000001d77358$3ad2aa80$b077ff80$@biopath.org> <0FD39A81-2536-4909-9729-51150C9E1FD6@icloud.com> <001d01d77361$50ae2150$f20a63f0$@biopath.org> <1625689472577.81801@health.nsw.gov.au> <002101d773f9$0e5c7430$2b155c90$@biopath.org>, Message-ID: <1625879273267.76314@health.nsw.gov.au> Hi John, There is anecdotal evidence that recycled xylene is of a better quality than the original purchased product. We have found it to be so. No processing nor staining problems unless there has been a recycling issue (as shown by the CBG xylene purity test). I suppose we have to access each reagent that is recycled and determine the risk of using it. For example, unless care is taken, recycling alcohol can be an issue (eg xylene contamination). We do not recycle alcohol for this reason. "When we are continuously challenged by pre-analytic variables in histotechnology why do labs continue to use recycled reagents? " >From your survey, has re-cycling xylene been found to be a major, or minor issue? We definitely need evidence that re-cycling is a risk. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________ From: John Garratt Sent: Saturday, 10 July 2021 05:31 To: Paula; Tony Henwood (SCHN); 'Erick Rodriguez' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] "cooked" biopsy Interesting discussion. At CPQA we recently started a H&E EQA program that includes fixation and processing in the feedback to participants and in a recent mini-survey I found that a third of labs use recycled product somewhere in the pre-analytic phase. When we are continuously challenged by pre-analytic variables in histotechnology why do labs continue to use recycled reagents? John On Thu, Jul 8, 2021 at 5:59 AM, Paula via Histonet > wrote: Hi Tony, Thank you for the procedure. I do send out the recycled xylene to have its analysis done by an outside company every month, and I'll put into place this testing procedure that you outlined below in place ase well. Paula -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood at health.nsw.gov.au] Sent: Wednesday, July 07, 2021 1:25 PM To: 'Erick Rodriguez'; Paula Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] "cooked" biopsy Hi Paula, We can check the purity of the xylene quite easily: Xylene Purity Test Procedure Note: The recommended and most accurate method of determining the purity of the recycled xylene is by doing a Gas Chromatography analysis. The following method can be used to obtain an acceptable confidence level in the purity of the recycled xylene (CBG Biotech). Testing Procedure 1. To a clean, dry 100 ml mixing cylinder graduate, add sufficient recovered xylene so that the bottom of the meniscus is aligned with the top edge of the 85 ml mark on the graduate. 2. Add water to the graduate until the bottom of the meniscus aligns with the top edge of the 100 ml mark on the graduate. At this point, 15 ml of water will have been added to 85 ml of recovered xylene. 3. Stopper the graduate and invert the mixture. Allow the mixture to settle, making sure that all of the water settles to the bottom of the graduate. No water should remain clinging to the sides of the graduate above the xylene/water separation point. This separation point should be near the 15 ml level of the graduate. (Note: xylene floats on top of the water). 4. Carefully inspect and record the point of separation between the water and xylene using the bottom of the meniscus as the separation point. 5. Subtract 15 ml from the quantity of water indicated in step 5. The remainder plus an additional 0.1 correction factor equals the percentage of recovered xylene impurities. EXAMPLE: Xylene/Water separation point is indicated to be 15.5 ml. (15.5 - 15) + 0.1 = 0.6% impurities. Therefore, the recovered xylene is 99.4% pure. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Paula via Histonet Sent: Thursday, 8 July 2021 04:53 To: 'Erick Rodriguez' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] "cooked" biopsy Thank you, everyone... I looked at my reagents and saw the color pink in the xylene, which tells me that there is water or too much water in it so I changed it. We recycle xylene, so I need to get the recycler looked at now. Thanks again, Paula -----Original Message----- From: Erick Rodriguez [mailto:rodriguez.erick at icloud.com] Sent: Wednesday, July 07, 2021 11:24 AM To: Paula Subject: Re: [Histonet] "cooked" biopsy Did you change the processor reagents before running your tissues? Cooked tissue usually means the tissue wasn?t dehydrated properly and the leftover water boiled and fried your tissue. I would double check the alcohols. > On Jul 7, 2021, at 11:00 AM, Paula via Histonet wrote: > > ?Hello, good day, > > > > Our pathologist is complaining about the tissues today that they are > "cooked, burnt, crushed, shrunken" those are the adjectives she is using. > > > > Can you tell me the cause? Usually, the work comes out beautiful but today > they are not. Nothing has changed on our processing times. > > > > What should I investigate? > > > > Thank you in advance, > > Paula > > Bio-Path Medica Group > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From john.garratt at ciqc.ca Fri Jul 9 23:40:13 2021 From: john.garratt at ciqc.ca (John Garratt) Date: Sat, 10 Jul 2021 04:40:13 +0000 Subject: [Histonet] "cooked" biopsy In-Reply-To: References: <000001d77358$3ad2aa80$b077ff80$@biopath.org> <0FD39A81-2536-4909-9729-51150C9E1FD6@icloud.com> <001d01d77361$50ae2150$f20a63f0$@biopath.org> <1625689472577.81801@health.nsw.gov.au> <002101d773f9$0e5c7430$2b155c90$@biopath.org> <1625879273267.76314@health.nsw.gov.au> Message-ID: <1-E68nidKA5bkiRE1FiCqgPaNt6aiO_RunvB2IUrGDNvBPayPsckPvFLO-RB4bWsFC3rCPydprafGvbf76rX5x0dasQGls8VKjNjcBFUJ9Q=@ciqc.ca> Thank you for the insights into recycling. Our surveys are based on labs achieving a quality of staining and processing that ensures accurate clinical interpretation and labs are generally doing an excellent job regardless of the reagents used which is a tribute to their QC. It would be great to see more standardization in histotechnology with the increased reliance on anatomic pathology to guide therapy, but I suspect that is a pipe dream. Here is an article that may be of interest https://link.springer.com/article/10.1007/s00428-020-02960-z John On Fri, Jul 9, 2021 at 7:18 PM, Jamie Watson wrote: > Hello everyone, > > Tony is correct that generally recycled xylene is purer than what you buy. I have been using recycled xylene since the early 80s from 4 different types of recyclers. > > The best practice is to have a small amount of new xylene constantly coming into the recycling stream. We always use purchased xylene on our H&E stainer to achieve this. Also about every 1-1.5 years replace all the xylene in the recycling stream. > > Alcohol recycling is too labor intensive and can be difficult to get the the percentage correct. > > I have never used recycled formalin. > > Jamie > > On July 9, 2021 6:13:13 PM "Tony Henwood (SCHN) via Histonet" wrote: > >> Hi John, >> >> There is anecdotal evidence that recycled xylene is of a better quality than the original purchased product. We have found it to be so. No processing nor staining problems unless there has been a recycling issue (as shown by the CBG xylene purity test). >> >> I suppose we have to access each reagent that is recycled and determine the risk of using it. For example, unless care is taken, recycling alcohol can be an issue (eg xylene contamination). We do not recycle alcohol for this reason. >> >> "When we are continuously challenged by pre-analytic variables in histotechnology why do labs continue to use recycled reagents? " >> >> From your survey, has re-cycling xylene been found to be a major, or minor issue? >> >> We definitely need evidence that re-cycling is a risk. >> >> Regards >> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) >> Principal Scientist, the Children?s Hospital at Westmead >> Adjunct Fellow, School of Medicine, University of Western Sydney >> Tel: 612 9845 3306 >> Fax: 612 9845 3318 >> Pathology Department >> the children's hospital at westmead >> Cnr Hawkesbury Road and Hainsworth Street, Westmead >> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA >> ________________________________ >> From: John Garratt >> Sent: Saturday, 10 July 2021 05:31 >> To: Paula; Tony Henwood (SCHN); 'Erick Rodriguez' >> Cc: histonet at lists.utsouthwestern.edu >> Subject: Re: [Histonet] "cooked" biopsy >> >> Interesting discussion. >> At CPQA we recently started a H&E EQA program that includes fixation and processing in the feedback to participants and in a recent mini-survey I found that a third of labs use recycled product somewhere in the pre-analytic phase. >> When we are continuously challenged by pre-analytic variables in histotechnology why do labs continue to use recycled reagents? >> >> John >> >> On Thu, Jul 8, 2021 at 5:59 AM, Paula via Histonet > wrote: >> Hi Tony, >> Thank you for the procedure. I do send out the recycled xylene to have its analysis done by an outside company every month, and I'll put into place this testing procedure that you outlined below in place ase well. >> Paula >> >> -----Original Message----- >> From: Tony Henwood (SCHN) [mailto:tony.henwood at health.nsw.gov.au] >> Sent: Wednesday, July 07, 2021 1:25 PM >> To: 'Erick Rodriguez'; Paula >> Cc: histonet at lists.utsouthwestern.edu >> Subject: Re: [Histonet] "cooked" biopsy >> >> Hi Paula, >> >> We can check the purity of the xylene quite easily: >> >> Xylene Purity Test Procedure >> >> Note: The recommended and most accurate method of determining the purity of the recycled xylene is by doing a Gas Chromatography analysis. The following method can be used to obtain an acceptable confidence level in the purity of the recycled xylene (CBG Biotech). >> >> Testing Procedure >> >> 1. To a clean, dry 100 ml mixing cylinder graduate, add sufficient recovered xylene so that the bottom of the meniscus is aligned with the top edge of the 85 ml mark on the graduate. >> >> 2. Add water to the graduate until the bottom of the meniscus aligns with the top edge of the 100 ml mark on the graduate. At this point, 15 ml of water will have been added to 85 ml of recovered xylene. >> >> 3. Stopper the graduate and invert the mixture. Allow the mixture to settle, making sure that all of the water settles to the bottom of the graduate. No water should remain clinging to the sides of the graduate above the xylene/water separation point. This separation point should be near the 15 ml level of the graduate. (Note: xylene floats on top of the water). >> >> 4. Carefully inspect and record the point of separation between the water and xylene using the bottom of the meniscus as the separation point. >> >> 5. Subtract 15 ml from the quantity of water indicated in step 5. The remainder plus an additional 0.1 correction factor equals the percentage of recovered xylene impurities. >> >> EXAMPLE: >> >> Xylene/Water separation point is indicated to be 15.5 ml. >> (15.5 - 15) + 0.1 = 0.6% impurities. >> Therefore, the recovered xylene is 99.4% pure. >> >> Regards >> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) >> Principal Scientist, the Children?s Hospital at Westmead >> Adjunct Fellow, School of Medicine, University of Western Sydney >> Tel: 612 9845 3306 >> Fax: 612 9845 3318 >> Pathology Department >> the children's hospital at westmead >> Cnr Hawkesbury Road and Hainsworth Street, Westmead >> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA >> >> ________________________________________ >> From: Paula via Histonet >> Sent: Thursday, 8 July 2021 04:53 >> To: 'Erick Rodriguez' >> Cc: histonet at lists.utsouthwestern.edu >> Subject: Re: [Histonet] "cooked" biopsy >> >> Thank you, everyone... >> I looked at my reagents and saw the color pink in the xylene, which tells me that there is water or too much water in it so I changed it. >> We recycle xylene, so I need to get the recycler looked at now. >> Thanks again, >> Paula >> >> -----Original Message----- >> From: Erick Rodriguez [mailto:rodriguez.erick at icloud.com] >> Sent: Wednesday, July 07, 2021 11:24 AM >> To: Paula >> Subject: Re: [Histonet] "cooked" biopsy >> >> Did you change the processor reagents before running your tissues? Cooked tissue usually means the tissue wasn?t dehydrated properly and the leftover water boiled and fried your tissue. I would double check the alcohols. >> >>> On Jul 7, 2021, at 11:00 AM, Paula via Histonet wrote: >>> >>> ?Hello, good day, >>> >>> Our pathologist is complaining about the tissues today that they are >>> "cooked, burnt, crushed, shrunken" those are the adjectives she is using. >>> >>> Can you tell me the cause? Usually, the work comes out beautiful but today >>> they are not. Nothing has changed on our processing times. >>> >>> What should I investigate? >>> >>> Thank you in advance, >>> >>> Paula >>> >>> Bio-Path Medica Group >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. >> >> Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. >> >> Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Tue Jul 13 10:51:16 2021 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 13 Jul 2021 11:51:16 -0400 Subject: [Histonet] Need a Resume Refresh? I Can Help!! Resumes - NOT just for job hunting anymore!! Message-ID: <000001d777fe$eb29c260$c17d4720$@earthlink.net> Hi Histopeeps, Resumes they're not just for job hunting anymore! Did you know it is a great idea to always keep your resume updated? Resumes and CVs are used for many things besides job hunting: Looking at a raise or promotion? . Does your resume list all of your accomplishments so that you can present them to your supervisor? Ever thought about giving a presentation or writing an article? . A great CV always makes a presentation much more polished. How about just for you? . It feels great to see how far you have come in your career and might help with direction for what's next. How about if you are transitioning? . Say going back to permanent work from travel or vice versa or want to move into another area of histology. And of course good old fashioned job hunting! Histopeeps, Let me help you get your resume tuned up!! It's free of charge as a service to my Histopeeps!! Here's what you need to do: Reply to this email with I'm in and we can get started. Job Hunting? Here are some of our Hottest opportunities. All of these are permanent positions. Most are RELIA Exclusives and all of my clients offer excellent compensation, benefits and most offer relocation/sign on bonuses!! RELIA HOT HISTOLOGY Opportunities OR Bend, OR Full-time Days Amazing team to work with FL Ft. Myers, FL Assay Development Specialist -IHC your passion? FL Ft. Myers, FL HT/HTL all shifts full&part time. ***I can help with your Florida license!*** CA Orange County, CA HT/HTL All shifts FULL& PART TIME AVAILABLE!! CA San Diego, CA Brand New Lab!! All shifts available! CA Modesto, CA IHC Specialist&Histotech positions state of the art lab!! I also have some great opportunities in: Virginia, Texas, Maryland and new opportunities coming in on a daily basis!! I look forward to assisting you!! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From tkngflght at yahoo.com Wed Jul 14 17:03:24 2021 From: tkngflght at yahoo.com (Cheryl) Date: Wed, 14 Jul 2021 22:03:24 +0000 (UTC) Subject: [Histonet] PathCentre (ThermoShandon) protocols - help? References: <7393119.276682.1626300204430.ref@mail.yahoo.com> Message-ID: <7393119.276682.1626300204430@mail.yahoo.com> Help?? Starting a refurbished PathCentre and was hoping someone could share a biopsy protocol for xylene processing?? Routine human biopsies with specials and some IHC and molecular down the road. Open to all -? Much appreciated!! Cheryl Kerry, HT(ASCP) From mjdessoye at commonwealthhealth.net Thu Jul 15 09:11:19 2021 From: mjdessoye at commonwealthhealth.net (Michael Dessoye) Date: Thu, 15 Jul 2021 10:11:19 -0400 Subject: [Histonet] Air quality monitoring Message-ID: Hi all...can anyone recommended a vendor for air quality monitoring/air exchange testing? Unhappy with my current vendor and looking for a change. Thanks! -- Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | Commonwealth Health Laboratory Services | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 From richard.wild at wanadoo.fr Fri Jul 16 02:01:35 2021 From: richard.wild at wanadoo.fr (richard.wild at wanadoo.fr) Date: Fri, 16 Jul 2021 09:01:35 +0200 Subject: [Histonet] Histonet Digest, Vol 212, Issue 7 In-Reply-To: References: Message-ID: <7d8996b5-268a-15a1-2e55-e491f6010f6d@wanadoo.fr> > Message: 1 > Date: Wed, 14 Jul 2021 22:03:24 +0000 (UTC) > From: Cheryl > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] PathCentre (ThermoShandon) protocols - help? > Message-ID: <7393119.276682.1626300204430 at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > > Help?? Starting a refurbished PathCentre and was hoping someone could share a biopsy protocol for xylene processing?? Routine human biopsies with specials and some IHC and molecular down the road. > Open to all -? > Much appreciated!! > Cheryl Kerry, HT(ASCP) Hi Cherryl it's a good machine (we have 3 of them) Here under some "official" protocole If unreadable, i'll send you pdf - just ask for it Richard Wild ----------------- SHANDON PATHCENTRE PROCESSING SCHEDULE RAPID BIOPSY PROGRAMME TOTAL DURATION 1hr 14mins STEP REAGENT CONC % TEMP ?C VAC/PRES TIME Hrs:Mins DRAIN AGIT 1 Formalin 10 45 V 00:01 15 S 2 Formalin 70 45 V 00:01 15 S 3 Alcohol 95 45 V 00:01 15 S 4 Alcohol 100 45 V 00:01 15 S 5 Alcohol 100 45 V 00:01 15 S 6 Alcohol 100 45 V 00:01 15 S 7 Alcohol 100 45 V 00:01 15 S 8 Xylene 45 V 00:01 15 S 9 Xylene 45 V 00:01 15 S 10 Xylene 45 V 00:01 15 S 11 Wax 60 V 00:01 60 S 12 Wax 60 V 00:01 60 S 13 Wax 60 V 00:01 60 S 14 Wax 60 V 00:01 60 S SHANDON PATHCENTRE PROCESSING SCHEDULE RAPID PROCESSING TOTAL DURATION 0hr 56mins STEP REAGENT CONC % TEMP ?C VAC/PRES TIME Hrs:Mins DRAIN AGIT 1 Formalin 10 45 V 00:02 15 S 2 Formalin 70 45 V 00:02 15 S 3 Alcohol 95 45 V 00:02 15 S 4 Alcohol 00:00 S 5 Alcohol 00:00 S 6 Alcohol 100 45 V 00:02 15 S 7 Alcohol 100 45 V 00:02 15 S 8 Xylene 00:00 S 9 Xylene 45 V 00:02 15 S 10 Xylene 45 V 00:02 15 S 11 Wax 60 00:00 S 12 Wax 60 00:00 S 13 Wax 60 V 00:02 60 S 14 Wax 60 V 00:02 60 S SHANDON PATHCENTRE PROCESSING SCHEDULE LARGE SURGICALS TOTAL DURATION 22hrs 34mins STEP REAGENT CONC % TEMP ?C VAC/PRES TIME Hrs:Mins DRAIN AGIT 1 Buffered Formalin 10 A N 02:00 15 S 2 Alcohol Formalin 10 A N 02:00 15 S 3 90% IMS * 60 A V 01:30 15 S 4 IMS 80 A V 01:30 15 S 5 IMS A V 01:30 15 S 6 IMS A V 01:30 15 S 7 Xylene/IMS A V 01:30 15 S 8 Xylene A V 01:30 30 S 9 Xylene A V 01:30 60 S 10 Xylene A V 01:30 60 S 11 Wax 60 V 01:00 120 S 12 Wax 60 V 01:00 120 S 13 Wax 60 V 01:00 120 S 14 Wax 60 V 01:30 120 S From Cheryl.Newman-Bodden at va.gov Fri Jul 16 10:22:14 2021 From: Cheryl.Newman-Bodden at va.gov (Newman-Bodden, Cheryl L.) Date: Fri, 16 Jul 2021 15:22:14 +0000 Subject: [Histonet] Job Opportunity in Tampa FL Message-ID: External JOA Link: https://www.usajobs.gov/GetJob/ViewDetails/607696600 VA in Tampa has several openings for HTL's with a four year degree. See listing above for details Cheryl Bodden, BS, HTL(ASCP) Histology Supervisor Supervisor Health Science Specialist Pathology and Laboratory Medicine James A. Haley Veterans Hospital 13000 Bruce B. Downs Blvd. Tampa, FL 33612 (813) 972-2000 ext. 7844 From dorianne.m.bonello at gov.mt Fri Jul 16 10:25:08 2021 From: dorianne.m.bonello at gov.mt (Bonello Dorianne M at Health-MDH) Date: Fri, 16 Jul 2021 15:25:08 +0000 Subject: [Histonet] frozen section problem Message-ID: <347cf0c62cf04f2b951891b2d320c73d@gov.mt> Dear all, We are experiencing freezing artifacts on our frozen sections. Basically, we are seeing cavity-like structures under the microscope, mostly elongated, especially when it's a frozen section on brain tissue. This is most probably happening due to ice crystal formation. We're not using cryospray, relying only on the cryobar boost function. Does anyone has a solution to this problem please? Regards, Dorianne Bonello Allied Health Practitioner (MLS) Histology Laboratory - Pathology Health-Mater Dei Hospital [cid:image001.jpg at 01D67184.63288530] T +356 +356 25456434 E dorianne.m.bonello at gov.mt Mater Dei Hospital, Triq id-Donaturi tad-Demm, l-Imsida, Malta MSD 2090 | Tel +356 2545 0000 | https://deputyprimeminister.gov.mt/en/MDH/Pages/Home.aspx | https://www.facebook.com/materdeihospital/ Think before you print. Kindly consider your environmental responsibility. This email and any files transmitted with it are confidential, may be legally privileged and intended solely for the use of the individual or entity to whom they are addressed. From rsrichmond at gmail.com Fri Jul 16 13:19:54 2021 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 16 Jul 2021 14:19:54 -0400 Subject: [Histonet] Frozen section problem In-Reply-To: References: Message-ID: Dorianne Bonello, Allied Health Practitioner (MLS), Histology Laboratory - Pathology Health-Mater Dei Hospital, on the island of Malta asks: > >>We are experiencing freezing artifacts on our frozen sections. > Basically, we are seeing cavity-like structures under the microscope, > mostly elongated, especially when it's a frozen section on brain tissue. > This is most probably happening due to ice crystal formation. We're not > using cryospray, relying only on the cryobar boost function.<< > > You need to freeze faster. A low-tech solution is a "heat extractor" - a > flat-bottomed mass of metal with a handle, kept cold in the cryostat, that > you press on top of the specimen after it's mounted on the chuck with > frozen section embedding medium. Here's a picture of it: https://tinyurl.com/4djk85xd This 82 year old pathologist would expect to trouble-shoot the problem himself. Not every day I get e-mail from Malta! If anyone on Histonet doesn't know where Malta is, it's an island nation in the Mediterranean Sea, south of Sicily. If you look at the Mater Dei web site, you'll see that it's in English, but with occasional lines of Maltese, the native language of the island, a form of North African Arabic, but always written in the Roman alphabet. Wikipedia has good resources for the island, the language, and the Mater Dei Hospital. Bob Richmond Samurai Pathologist Maryville, Tennessee, USA From jkiernan at uwo.ca Fri Jul 16 15:43:22 2021 From: jkiernan at uwo.ca (John Kiernan) Date: Fri, 16 Jul 2021 20:43:22 +0000 Subject: [Histonet] frozen section problem In-Reply-To: <347cf0c62cf04f2b951891b2d320c73d@gov.mt> References: <347cf0c62cf04f2b951891b2d320c73d@gov.mt> Message-ID: Yes, definitely ice crystal holes! If the tissues are unfixed you will have to freeze much more rapidly (isopentane cooled with liquid nitrogen.) If fixed in formaldehyde, cryoprotect by immersing the pieces in 20% sucrose, until they sink. John Kiernan Anatomy & Cell Biology, UWO London, Canada = = = ________________________________ From: Bonello Dorianne M at Health-MDH via Histonet Sent: July 16, 2021 11:25 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] frozen section problem Dear all, We are experiencing freezing artifacts on our frozen sections. Basically, we are seeing cavity-like structures under the microscope, mostly elongated, especially when it's a frozen section on brain tissue. This is most probably happening due to ice crystal formation. We're not using cryospray, relying only on the cryobar boost function. Does anyone has a solution to this problem please? Regards, Dorianne Bonello Allied Health Practitioner (MLS) Histology Laboratory - Pathology Health-Mater Dei Hospital [cid:image001.jpg at 01D67184.63288530] T +356 +356 25456434 E dorianne.m.bonello at gov.mt Mater Dei Hospital, Triq id-Donaturi tad-Demm, l-Imsida, Malta MSD 2090 | Tel +356 2545 0000 | https://deputyprimeminister.gov.mt/en/MDH/Pages/Home.aspx | https://www.facebook.com/materdeihospital/ Think before you print. Kindly consider your environmental responsibility. This email and any files transmitted with it are confidential, may be legally privileged and intended solely for the use of the individual or entity to whom they are addressed. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Fri Jul 16 15:59:53 2021 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 16 Jul 2021 20:59:53 +0000 Subject: [Histonet] brain freeze artifact Message-ID: <48E053DDF6CE074DB6A7414BA05403F80202C1E5D7@HRHEX03-HOS.holyredeemer.local> First of all, freezing spray should NEVER be used in a cryostat. It produces dangerous aerosolized pathogens that linger in the air, just waiting to infect someone. There should be no exceptions. As to the ice artifact problem, not surprising to see this in brain since it is so fragile and often edematous. Your best bet to eliminate freeze artifact is to "snap freeze" the tissue using the technique used in muscle biopsy samples. A metal sample cup containing 2-Methyl Butane (Isopentane) is suspended in Liquid Nitrogen, stirring until it becomes a thickened, white slurry. The mounted tissue sample is immersed in this slurry to freeze, then placed in the cryostat to come up to cryostat temps before cutting. That is the short and sweet version, and there are many published versions of the technique. It is cumbersome, but produces no freezing artifact. Best of luck, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-3874 ------------------------------ Message: 3 Date: Fri, 16 Jul 2021 15:25:08 +0000 From: Bonello Dorianne M at Health-MDH To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] frozen section problem Dear all, We are experiencing freezing artifacts on our frozen sections. Basically, we are seeing cavity-like structures under the microscope, mostly elongated, especially when it's a frozen section on brain tissue. This is most probably happening due to ice crystal formation. We're not using cryospray, relying only on the cryobar boost function. Does anyone has a solution to this problem please Regards, Dorianne Bonello Allied Health Practitioner (MLS) Histology Laboratory - Pathology Health-Mater Dei Hospital From philip_manfre at merck.com Fri Jul 16 16:54:23 2021 From: philip_manfre at merck.com (Manfre, Philip) Date: Fri, 16 Jul 2021 21:54:23 +0000 Subject: [Histonet] frozen section problem Message-ID: Hi Dorianne, You need to freeze your tissue faster. Ideally, isopentane placed in a metal cup, that is that is then frozen in a dewar of liquid nitrogen, works best. The isopentane, once frozen, is thawed a little with a metal rod to produce a small liquid pool and your tissue is placed in this for about one minute. You need some equipment for this procedure, such as the metal cup that can sit inside a small, open dewar of liquid nitrogen. Alternatively, you can freeze directly in liquid nitrogen, though you need to beware of the tissue fracturing due to the sudden and extreme temperature reduction. Slower freezing of tissue (sitting on dry ice, etc.) allows ice crystals to form in the tissue, creating the vacuoles you describe. I hope this helps. Phil. Philip Manfre, BA, HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP81-406 770 Sumneytown Pike West Point, PA 19486 215-652-9750 philip_manfre at merck.com -----Original Message----- From: Bonello Dorianne M at Health-MDH via Histonet Sent: Friday, July 16, 2021 11:25 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] frozen section problem EXTERNAL EMAIL ? Use caution with any links or file attachments. Dear all, We are experiencing freezing artifacts on our frozen sections. Basically, we are seeing cavity-like structures under the microscope, mostly elongated, especially when it's a frozen section on brain tissue. This is most probably happening due to ice crystal formation. We're not using cryospray, relying only on the cryobar boost function. Does anyone has a solution to this problem please? Regards, Dorianne Bonello Allied Health Practitioner (MLS) Histology Laboratory - Pathology Health-Mater Dei Hospital [cid:image001.jpg at 01D67184.63288530] T +356 +356 25456434 E dorianne.m.bonello at gov.mt Mater Dei Hospital, Triq id-Donaturi tad-Demm, l-Imsida, Malta MSD 2090 | Tel +356 2545 0000 | https://deputyprimeminister.gov.mt/en/MDH/Pages/Home.aspx | https://www.facebook.com/materdeihospital/ Think before you print. Kindly consider your environmental responsibility. This email and any files transmitted with it are confidential, may be legally privileged and intended solely for the use of the individual or entity to whom they are addressed. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From tony.henwood at health.nsw.gov.au Fri Jul 16 17:31:38 2021 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 16 Jul 2021 22:31:38 +0000 Subject: [Histonet] frozen section problem In-Reply-To: References: Message-ID: <1626474691777.17386@health.nsw.gov.au> We have had this issue previously. We tracked it down to the brain biopsies arriving in "Isotonic" saline (which is really not isotonic). See: Henwood, A., (2007) ?Adverse effect of saline on brain intraoperative (frozen section) Histology? J Histotechnol 30(3):193. Ask the surgeons to send the biopsies to the lab on damp shiny (non-absorbent) card. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Manfre, Philip via Histonet Sent: Saturday, 17 July 2021 07:54 To: Bonello Dorianne M at Health-MDH; (histonet at lists.utsouthwestern.edu) Subject: Re: [Histonet] frozen section problem Hi Dorianne, You need to freeze your tissue faster. Ideally, isopentane placed in a metal cup, that is that is then frozen in a dewar of liquid nitrogen, works best. The isopentane, once frozen, is thawed a little with a metal rod to produce a small liquid pool and your tissue is placed in this for about one minute. You need some equipment for this procedure, such as the metal cup that can sit inside a small, open dewar of liquid nitrogen. Alternatively, you can freeze directly in liquid nitrogen, though you need to beware of the tissue fracturing due to the sudden and extreme temperature reduction. Slower freezing of tissue (sitting on dry ice, etc.) allows ice crystals to form in the tissue, creating the vacuoles you describe. I hope this helps. Phil. Philip Manfre, BA, HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP81-406 770 Sumneytown Pike West Point, PA 19486 215-652-9750 philip_manfre at merck.com -----Original Message----- From: Bonello Dorianne M at Health-MDH via Histonet Sent: Friday, July 16, 2021 11:25 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] frozen section problem EXTERNAL EMAIL ? Use caution with any links or file attachments. Dear all, We are experiencing freezing artifacts on our frozen sections. Basically, we are seeing cavity-like structures under the microscope, mostly elongated, especially when it's a frozen section on brain tissue. This is most probably happening due to ice crystal formation. We're not using cryospray, relying only on the cryobar boost function. Does anyone has a solution to this problem please? Regards, Dorianne Bonello Allied Health Practitioner (MLS) Histology Laboratory - Pathology Health-Mater Dei Hospital [cid:image001.jpg at 01D67184.63288530] T +356 +356 25456434 E dorianne.m.bonello at gov.mt Mater Dei Hospital, Triq id-Donaturi tad-Demm, l-Imsida, Malta MSD 2090 | Tel +356 2545 0000 | https://secure-web.cisco.com/108H5-f5Ylbr6vXriY71-fp4JDQxST7FvP8i3GNqBPS05qZlvu_1O6tMXRjgYucUESUfrxOSGo-3VSj277AHeUKp72a2u23a8E1gThp8T_C_qgaCkXVHMnAxY-YyXqqjb0pEmmV-J74L1UJXhwqdO88nHIMcK0Y7_wGmWPWFgvvMfmer4a-NFgWAPDQDYgBdnmJ-UysbSbmKuEVVZzOZFDBgn-f2wXW3JKjvg0h2q9rXdcxCMqWYFuQFY1xiKWJHR9NcNUphJ6xHBSRJSogoK53iKRSk1tEyOBPErMdipZOz4PXDp29G4cAm7LIrI_3TQWhJLOaI2wZTc0wJ7yRUScq8nh2P3hrxSuBaL3ivOfsU9eUtNGDZDo9XeNFEcGmivY-fPo1XKWjQy6pmHUaksuix_Jp9AvaCBh0IWf_zuPVGEv2oeS9aYQXub1b9B0QpJFqSicysNAHY65JYUzhjHXxuuvsaJ-m6zou-55OWaT8dK3Cjh6bvXTnQWzS3C4Uya/https%3A%2F%2Fdeputyprimeminister.gov.mt%2Fen%2FMDH%2FPages%2FHome.aspx | https://www.facebook.com/materdeihospital/ Think before you print. Kindly consider your environmental responsibility. This email and any files transmitted with it are confidential, may be legally privileged and intended solely for the use of the individual or entity to whom they are addressed. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From dorianne.m.bonello at gov.mt Sun Jul 18 01:19:22 2021 From: dorianne.m.bonello at gov.mt (Bonello Dorianne M at Health-MDH) Date: Sun, 18 Jul 2021 06:19:22 +0000 Subject: [Histonet] F/S issue Message-ID: Dear all, Thank you for your feedback regarding the frozen section issue. Very helpful. Regards, Dorianne Bonello Allied Health Practitioner (MLS) Histology Laboratory - Pathology Health-Mater Dei Hospital [cid:image001.jpg at 01D67184.63288530] T +356 +356 25456434 E dorianne.m.bonello at gov.mt Mater Dei Hospital, Triq id-Donaturi tad-Demm, l-Imsida, Malta MSD 2090 | Tel +356 2545 0000 | https://deputyprimeminister.gov.mt/en/MDH/Pages/Home.aspx | https://www.facebook.com/materdeihospital/ Think before you print. Kindly consider your environmental responsibility. This email and any files transmitted with it are confidential, may be legally privileged and intended solely for the use of the individual or entity to whom they are addressed. From relia1 at earthlink.net Tue Jul 20 11:02:50 2021 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 20 Jul 2021 12:02:50 -0400 Subject: [Histonet] ICYMI: My latest post - Top Tips for Working with Recruiters p.s. don't forget about my free resume tune ups! Message-ID: <00b401d77d80$b1f24710$15d6d530$@earthlink.net> Hi Histopeeps, I wanted to give you a heads up!! My latest blog post is up on Fixation on Histology. I think it contains some great observations on how to get the most out using a recruiter for your job search. As a matter of fact it could be useful to everyone not just my histopeeps - I am hoping this is helpful to you and your friends and family. My latest article is entitled: "Top Tips for Working with Recruiters" This is the link to my article on the NSH's Fixation on Histology Blog and it was also published in the latest edition of the NSH Career Newsletter. Here is the link to the article: https://www.nsh.org/blogs/pam-barker/2021/07/16/tips-for-working-with-a-recr uiter If you have a minute to read it I would love to hear what you think. If you can't get to the article with this link let me know and I will send you a copy of it. Histopeeps, we also have amazing opportunities nationwide in! California: . Orange County - all shifts learn new things!! . Ventura - run your own private lab . San Diego - leadership and tech positions all shifts! . Modesto - leading edge environment Florida: . Ft. Myers - leadership and tech positions all shifts . Ft. Myers - Assay Development Specialist . Panama City- live at the beach!!! NC - Wilmington MD - Annapolis TX - Austin VA - Norfolk Some of these positions are RELIA exclusives! You won't see them anyplace else! My clients offer competitive pay rates, excellent benefits and in most cases Relocation assistance and/or a sign on bonus. They are ready to interview and hire!!! We have new opportunities coming in from all over the U.S. daily. If you don't see your location PLEASE let me know. I might have the perfect position for you and might come in BEFORE the next newsletter!! Please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral reward! I LOVE TO GIVE REFERRAL REWARDS!! What more could you ask for? If You or Anyone You Know Might Be Interested In a New Opportunity, Please Contact Me ASAP If you want to chat ASAP call or text me on my cell At 407-353-5070. If you want some additional information or to set up a time to chat please call me toll free at 866-607-3542 or email me at relia1 at earthlink.net My clients are ready to interview and hire right away!!! And my phone is still ringing off the hook so if you don't see the location you want yet drop me a line so I can let you know when something DOES come up. I could be talking to a client about your next opportunity RIGHT NOW!! Have a great day. I look forward to hearing back from you. Thanks-Pam Right Time, Right Place, Right Move with RELIA! *16 Years!* Celebrating 16 years of service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From melissa at alliedsearchpartners.com Tue Jul 20 14:17:20 2021 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Tue, 20 Jul 2021 19:17:20 +0000 Subject: [Histonet] Histology Supervisor Job in Atlanta-Day Shift Message-ID: Hello, I hope everyone is doing well! Looking forward to seeing face to face meetings and events again in the future. I have a Full Time/Day Shift Histology Supervisor job for Permanent Full Time hire in Atlanta, GA. Please reply for more details and to schedule a call with me to discuss. Melissa Owens, CHP-ASA Allied Search Partners AN MRINETWORK MEMBER Direct (Call) Line: 407.697.1175 Toll Free: 888.388.7571 ext. 102 From raycher.caroline at marshfieldclinic.org Fri Jul 23 12:01:14 2021 From: raycher.caroline at marshfieldclinic.org (Raycher, Caroline L) Date: Fri, 23 Jul 2021 17:01:14 +0000 Subject: [Histonet] Histology Training Message-ID: The Marshfield Labs Histotechnician (HT) Student Program is seeking applicants for its 2021-2022 practicum class. The 11-month HT Practicum is ideal for people who have completed an associate or bachelor?s degree in biology and are looking for additional skills that will allow them to pursue a career in a clinical laboratory. Program Information, including prerequisites, can be found on the Marshfield Labs? Laboratory Student Programs web page. Application materials are available on the same web page. The application deadline has been extended. Questions can be sent to labeduc at marshfieldclinic.org . Carrie Raycher, HT(ASCP) HT Program Coordinator ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From raycher.caroline at marshfieldclinic.org Fri Jul 23 12:04:28 2021 From: raycher.caroline at marshfieldclinic.org (Raycher, Caroline L) Date: Fri, 23 Jul 2021 17:04:28 +0000 Subject: [Histonet] supply Chain Xylene Message-ID: <63ff0a21523e49f4909bf79e6849cc21@marshfieldclinic.org> Looking for xylene in 55 gallon drums. Our supplier has discontinued them. Is this a trend? Carrie Raycher, HT(ASCP) Marshfield Laboratories raycher.caroline at marshfieldclinic.org ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From sandra.cheasty at wisc.edu Fri Jul 23 18:47:04 2021 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Fri, 23 Jul 2021 23:47:04 +0000 Subject: [Histonet] Exciting position at the University of Wisconsin~Madison Message-ID: Hello all, The School of Veterinary Medicine at the University of Wisconsin, Madison has a full-time position for a Necropsy Technician. The position is responsible for assisting the veterinary pathologists in the biopsy and autopsy service - much like a human hospital but with a much wider variety of species from the veterinary teaching hospital, (dogs, cats, horses, cows), the DNR, (wolves, deer, raccoons, birds), and the Milwaukee County Zoo, (fish, primates, kudu, rhinos, penguins, and many other exotic species. I'm not gonna lie; It's pretty cool. This is a terrific group of co-workers and pathologists who are dedicated to furthering the knowledge of veterinary medicine. Here's the link... NECROPSY TECHNICIAN at UW-Madison (wisc.edu) Cheers, Sandy Sandra Cheasty, HT(ASCP), Emerita School of Veterinary Medicine University of Wisconsin From Nancy.Schmitt at mercyhealth.com Mon Jul 26 15:20:36 2021 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Mon, 26 Jul 2021 20:20:36 +0000 Subject: [Histonet] cytology billing Message-ID: Hello- Looking for direction on billing practices - specifically regarding non-gyn specimens that are screened by a cytotech and then passed on to the pathologist for sign-out. Can you bill twice for that? I am thinking not???thank you in advance? MercyOne Dubuque Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From idimenstein at hotmail.com Tue Jul 27 21:50:49 2021 From: idimenstein at hotmail.com (Izak Dimenstein) Date: Wed, 28 Jul 2021 02:50:49 +0000 Subject: [Histonet] Histonet Digest, Vol 212, Issue 14 In-Reply-To: References: Message-ID: In contrast to gynecological cytology, nongynecological cytology (NGYN) does not have a separate CPT code for screening. Details in my book's Procedural Coding in Anatomic Pathology Cytopathology section, pp. 162-176. Izak Dimenstein ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, July 27, 2021 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 212, Issue 14 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From jwwalker at rrmc.org Wed Jul 28 10:05:06 2021 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Wed, 28 Jul 2021 15:05:06 +0000 Subject: [Histonet] cytology billing In-Reply-To: References: Message-ID: Well, that is a trick question and depends on how your lab is setup. Most non-gyn CPT codes contain a technical (TC) and a professional component (PC). In my hospital lab, we bill the technical component but our contacted pathology group bills out the professional component. The TC from our perspective covers the cytotech's review. Keep in mind, that there is no CLIA requirement for a cytotech to prescreen a non-gyn specimen. Does this help? Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology and Interim Phlebotomy Manager P 802.747.1790 F 802.747.6525 joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Nancy Schmitt via Histonet Sent: Monday, July 26, 2021 4:21 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] cytology billing [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. Hello- Looking for direction on billing practices - specifically regarding non-gyn specimens that are screened by a cytotech and then passed on to the pathologist for sign-out. Can you bill twice for that? I am thinking not???thank you in advance? MercyOne Dubuque Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!I87qwjxLstg3H_X5!o9klCfkynm2765rSPOv0lji4TV5jm1QjBsVo1h3PQVw7QOtay-zYE04G0yub0oA$ [https://www.rrmc.org/app/files/public/3159/ValesEmailSig2020.jpg] From relia1 at earthlink.net Thu Jul 29 09:26:50 2021 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 29 Jul 2021 10:26:50 -0400 Subject: [Histonet] Exciting Opportunities in Florida and California with bonuses up to 10K!! Message-ID: <000001d78485$c6355620$52a00260$@earthlink.net> Hello Histopeeps, I hope you are having a great week! I am Sooo excited about the opportunities I am working on and here?s why: 1. The Florida license is easier than EVER to get. Here?s the link: www.floridasclinicallabs.gov 2. My clients are excited to meet you and ready to make a decision right away. 3. Most of these positions offer up to 10K in bonuses and relocation assistance!! 4. If you have plans made for time off you don?t have to cancel them!! 5. These are some of the top employers in their areas and I have techs that have been working in some of these places for YEARS! Histopeeps!! I have some amazing job opportunities in: Florida ? Panama City Dermpath/Days ? Ft. Myers Dermpath/Days ? Ft. Myers Night Shift Supervisor ? Ft. Myers IHC specialist/Days ? Ft. Myers Histotech/ all shifts Full time part time per diem ? Ft. Myers Histotech /All Shifts New Grads welcome California ? Aliso Viejo Histotech/ all shifts Full time part time per diem ? Aliso Viejo Histotech /All Shifts New Grads welcome ? San Diego Histotech/ all shifts Full time part time per diem ? San Diego Histology Supervisor ? Brand New Lab!! ? Modesto Histotech ? nights ? Modesto IHC Specialist And New Opportunities coming in on a daily basis!! All of these clients are offering full time permanent positions with excellent compensation packages including VERY competitive pay rates, fantastic benefits, relocation and/or sign on bonuses (Up to 10K) and a great team to work with!! I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542, on my cell at 407-353-5070 or relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From relia1 at earthlink.net Thu Jul 29 09:57:10 2021 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 29 Jul 2021 10:57:10 -0400 Subject: [Histonet] Exciting Opportunities in Florida and California with bonuses up to 10K!! Message-ID: <00e901d7848a$025c0fa0$07142ee0$@earthlink.net> Hello Histopeeps, I hope you are having a great week! I am Sooo excited about the opportunities I am working on and here?s why: 1. The Florida license is easier than EVER to get. Here?s the link: www.floridasclinicallabs.gov 2. My clients are excited to meet you and ready to make a decision right away. 3. Most of these positions offer up to 10K in bonuses and relocation assistance!! 4. If you have plans made for time off you don?t have to cancel them!! 5. These are some of the top employers in their areas and I have techs that have been working in some of these places for YEARS! Histopeeps!! I have some amazing job opportunities in: Florida ? Panama City Dermpath/Days ? Ft. Myers Dermpath/Days ? Ft. Myers Night Shift Supervisor ? Ft. Myers IHC specialist/Days ? Ft. Myers Histotech/ all shifts Full time part time per diem ? Ft. Myers Histotech /All Shifts New Grads welcome California ? Aliso Viejo Histotech/ all shifts Full time part time per diem ? Aliso Viejo Histotech /All Shifts New Grads welcome ? San Diego Histotech/ all shifts Full time part time per diem ? San Diego Histology Supervisor ? Brand New Lab!! ? Modesto Histotech ? nights ? Modesto IHC Specialist And New Opportunities coming in on a daily basis!! All of these clients are offering full time permanent positions with excellent compensation packages including VERY competitive pay rates, fantastic benefits, relocation and/or sign on bonuses (Up to 10K) and a great team to work with!! I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542, on my cell at 407-353-5070 or relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From relia1 at earthlink.net Thu Jul 29 10:02:48 2021 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 29 Jul 2021 11:02:48 -0400 Subject: [Histonet] Exciting Opportunities in Florida and California with bonuses up to 10K!! Message-ID: <013c01d7848a$cbeab970$63c02c50$@earthlink.net> Hello Histopeeps, I hope you are having a great week! I am Sooo excited about the opportunities I am working on and here?s why: 1. The Florida license is easier than EVER to get. Here?s the link: www.floridasclinicallabs.gov 2. My clients are excited to meet you and ready to make a decision right away. 3. Most of these positions offer up to 10K in bonuses and relocation assistance!! 4. If you have plans made for time off you don?t have to cancel them!! 5. These are some of the top employers in their areas and I have techs that have been working in some of these places for YEARS! Histopeeps!! I have some amazing job opportunities in: Florida ? Panama City Dermpath/Days ? Ft. Myers Dermpath/Days ? Ft. Myers Night Shift Supervisor ? Ft. Myers IHC specialist/Days ? Ft. Myers Histotech/ all shifts Full time part time per diem ? Ft. Myers Histotech /All Shifts New Grads welcome California ? Aliso Viejo Histotech/ all shifts Full time part time per diem ? Aliso Viejo Histotech /All Shifts New Grads welcome ? San Diego Histotech/ all shifts Full time part time per diem ? San Diego Histology Supervisor ? Brand New Lab!! ? Modesto Histotech ? nights ? Modesto IHC Specialist And New Opportunities coming in on a daily basis!! All of these clients are offering full time permanent positions with excellent compensation packages including VERY competitive pay rates, fantastic benefits, relocation and/or sign on bonuses (Up to 10K) and a great team to work with!! I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542, on my cell at 407-353-5070 or relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From PAGE.BALUCH at asu.edu Thu Jul 29 16:30:20 2021 From: PAGE.BALUCH at asu.edu (Page Baluch) Date: Thu, 29 Jul 2021 21:30:20 +0000 Subject: [Histonet] List of best resources for histotechnologists Message-ID: Hi All, Next week we will be having the Microscopy and Microanalysis 2021 virtual conference and I am organizing a session as part of the Technicians' Forum called Histology Helpline. The session will have a group of panelists who represent authors, listserv organizers and application scientists who will provide helpful resource information to histologists. Our own Timothy Morken will be there to talk about Histonet! In addition to the roundtable discussion, we wanted to provide a list of the best books, websites, listservs or other resources that established histologists have used in the past. If you are interested in helping, please send me the name of any resource that might benefit someone less established in the field. Thanks for your help! Page D. Page Baluch, PhD Assistant Director | Research Scientist Knowledge Enterprise | Biosciences Arizona State University From Timothy.Morken at ucsf.edu Thu Jul 29 17:25:05 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 29 Jul 2021 22:25:05 +0000 Subject: [Histonet] EM immunolabeling for kappa, lambda light chains in amyloid? Message-ID: Can anyone give me any tips on EM immuno-gold labeling for kappa and lambda light chains in amyloid cases? We use Eponate12 for embedding. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center