From ASelf at tidelandshealth.org Thu Jan 7 13:22:22 2021 From: ASelf at tidelandshealth.org (Amy Self) Date: Thu, 7 Jan 2021 19:22:22 +0000 Subject: [Histonet] Joint Commission Standards for Pathology Message-ID: Good Afternoon and I hope that everyone had a safe and Happy New Year, Question: Does Joint Commission offer a checklist like CAP does that is specific to pathology? Thanks, Amy Self Senior Histology Technologist Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 Office: (843) 520-8711 aself at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From Timothy.Morken at ucsf.edu Thu Jan 7 13:34:35 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 7 Jan 2021 19:34:35 +0000 Subject: [Histonet] Joint Commission Standards for Pathology In-Reply-To: References: Message-ID: Amy, they don't have a specific AP and General checklist like CAP has. They have a Standards book that lists all the requirements and you need to go thru and figure out which apply to your area. It is also on line at the TJC site. The thing is that TJC covers the entire hospital, not just the labs, so much of it is for other areas besides the lab. They don't do inspections in the way CAP does it. CAP will camp out in your lab for days going over every detail on the checklist. Instead TJC uses "Tracers" and pulls random patient files for random dates of the year and then asks for everything related to those files. So any lab work, reports, QC results, equipment records, personnel records (qualifications, competencies, etc) of anyone who worked on tests for those patients. That is really the part that has to be focused on. In the last 5 inspections I've had from TJC they spent about 30 min in the lab each time. The rest of the time was going over tracer records. In the end it checks the same things, but in a very different way. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Amy Self via Histonet Sent: Thursday, January 07, 2021 11:22 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Joint Commission Standards for Pathology Good Afternoon and I hope that everyone had a safe and Happy New Year, Question: Does Joint Commission offer a checklist like CAP does that is specific to pathology? Thanks, Amy Self Senior Histology Technologist Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 Office: (843) 520-8711 aself at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=v__WI8KN5S058FvjkjGLPeXWuiR09WJe6dXFJr0VOSc&s=5-ix0KHOTuJHpbWl1_XB9Cs-bKZ3ONUyiw0KAMLF5ps&e= From sandra.cheasty at wisc.edu Fri Jan 8 10:08:21 2021 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Fri, 8 Jan 2021 16:08:21 +0000 Subject: [Histonet] New Tissue Processor? Message-ID: Hello all, Our lab is looking to replace our 10 year old VIP6. We process anywhere from 50 to 200 cassettes daily. Any recommendations are appreciated. Thank you, Sandra Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From mwhitaker1 at gmail.com Fri Jan 8 10:59:40 2021 From: mwhitaker1 at gmail.com (Melissa Whitaker) Date: Fri, 8 Jan 2021 11:59:40 -0500 Subject: [Histonet] New Tissue Processor? Message-ID: Hello Sandra, My name is Melissa Whitaker and I work with a company called Dakewe. We offer a full line of pathology devices and in particular one of our best models happens to be our tissue processor the HP300. If you would like more information, please let me know and I would be happy to discuss the instrument with you. Best Regards, Melissa A. Whitaker, BA, HTL(ASCP)cm Dakewe Medical, Product Specialist Www.Dakewe medical.com Sent from my iPhone > On Jan 8, 2021, at 11:24 AM, Sandra Cheasty via Histonet wrote: > From John.Shelton at UTSouthwestern.edu Fri Jan 8 11:04:21 2021 From: John.Shelton at UTSouthwestern.edu (John Shelton) Date: Fri, 8 Jan 2021 17:04:21 +0000 Subject: [Histonet] New Tissue Processor? In-Reply-To: References: Message-ID: Forum is for consumer traffic alone, and for the benefit of the front-line histologist. We are happy to have you as a member that monitors the boards, but please refrain from marketing practices. John M Shelton Core Operations Manager Histonet List Owner & Moderator UT Southwestern Histo Pathology Core 5323 Harry Hines Blvd Dallas, Texas 75390-8573 (214)648-1451 Visit Histonet Archives at: http://www.histosearch.com/histonet.html ________________________________ From: zzz_Tag_histonet-lists Sent: Friday, January 8, 2021 10:59 AM To: zzz_Tag_histonet-lists Subject: Re: [Histonet] New Tissue Processor? Hello Sandra, My name is Melissa Whitaker and I work with a company called Dakewe. We offer a full line of pathology devices and in particular one of our best models happens to be our tissue processor the HP300. If you would like more information, please let me know and I would be happy to discuss the instrument with you. Best Regards, Melissa A. Whitaker, BA, HTL(ASCP)cm Dakewe Medical, Product Specialist Www.Dakewe medical.com Sent from my iPhone > On Jan 8, 2021, at 11:24 AM, Sandra Cheasty via Histonet wrote: > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ UT Southwestern Medical Center The future of medicine, today. From SteveM at mcclainlab.com Fri Jan 8 12:59:20 2021 From: SteveM at mcclainlab.com (Steve McClain) Date: Fri, 8 Jan 2021 18:59:20 +0000 Subject: [Histonet] Histonet Digest, Vol 206, Issue 1 New Tissue Processor? (Sandra Cheasty) In-Reply-To: References: Message-ID: <31BEBA9A-3CF5-4AB0-B0FA-F505D73ECD6D@mcclainlab.com> While (conceptually) looking forward to the virtues of new and improved modern tissue processors w WiFi and SMS messaging capability, I fully expect to be operating the 1979 K-series for years to come. I reckon we may be behind the times, but with regular annual maintenance, our VIP5 and even more reliable K-series VIP processors refuse to die. These run/process 6 days a week and twice a day, if/when rapid processing is needed. PS we also have two spare operational (but slightly hacked into) K-series processors from when we were contemplating an experiment in cold processing using refrigerated alcohols and xylenes to preserve some obscure research antigens. In hindsight, it was a goofy academic exercise and soon was abandoned and rarely mentioned, after we (meaning Joel) adjusted the antigen retrieval. Now seriously, has anyone else ever modified their tissue processor with a Saws-all? Please reply confidentially. Steve Steve A. McClain, MD 631-361-4000 Cell 631-926-3655 From deedeebrina at aol.com Fri Jan 8 13:47:02 2021 From: deedeebrina at aol.com (Dee Neamand) Date: Fri, 8 Jan 2021 14:47:02 -0500 Subject: [Histonet] Pay range for histology supervisor References: <5507F394-BC31-4619-8AAE-81D3DCB8334B.ref@aol.com> Message-ID: <5507F394-BC31-4619-8AAE-81D3DCB8334B@aol.com> Hello. Can anyone give me some ideas for appropriate pay range for a histology supervisor that is also a grossing technician? Thanks, Dee Neamand Sent from my iPhone From Timothy.Morken at ucsf.edu Fri Jan 8 14:03:45 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 8 Jan 2021 20:03:45 +0000 Subject: [Histonet] Pay range for histology supervisor In-Reply-To: <5507F394-BC31-4619-8AAE-81D3DCB8334B@aol.com> References: <5507F394-BC31-4619-8AAE-81D3DCB8334B.ref@aol.com> <5507F394-BC31-4619-8AAE-81D3DCB8334B@aol.com> Message-ID: Dee, it depends on where you are.... Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Dee Neamand via Histonet Sent: Friday, January 08, 2021 11:47 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pay range for histology supervisor Hello. Can anyone give me some ideas for appropriate pay range for a histology supervisor that is also a grossing technician? Thanks, Dee Neamand Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=NaX6XjBugVH18gPZlDuE5RjCCnpWHLOBXNYBYKNXv8c&s=TQLXICIv79QWw90-3SOTCzVnOm1ZFje63WSLogxwLwU&e= From Timothy.Morken at ucsf.edu Fri Jan 8 16:48:30 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 8 Jan 2021 22:48:30 +0000 Subject: [Histonet] FW: Pay range for histology supervisor In-Reply-To: <0A58B6B9-DD92-46F0-AD4D-109D5D034D7E@aol.com> References: <0A58B6B9-DD92-46F0-AD4D-109D5D034D7E@aol.com> Message-ID: I?m in central PA Sent from my iPhone > On Jan 8, 2021, at 3:03 PM, Morken, Timothy wrote: > > ?Dee, it depends on where you are.... > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology UC San Francisco Medical Center > > -----Original Message----- > From: Dee Neamand via Histonet > Sent: Friday, January 08, 2021 11:47 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Pay range for histology supervisor > > Hello. Can anyone give me some ideas for appropriate pay range for a histology supervisor that is also a grossing technician? > > Thanks, > Dee Neamand > > Sent from my iPhone > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=NaX6XjBugVH18gPZlDuE5RjCCnpWHLOBXNYBYKNXv8c&s=TQLXICIv79QWw90-3SOTCzVnOm1ZFje63WSLogxwLwU&e= From Timothy.Morken at ucsf.edu Fri Jan 8 17:48:28 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 8 Jan 2021 23:48:28 +0000 Subject: [Histonet] Pre-made chrome alum slides source? Message-ID: Our source for pre-made chrome alum-subbed slides does not exist anymore. Does anyone know of a company that produces these? (American Master Tech had them but when StatLab bought them they discontinued the chrome alum slides) We used these for EM thick sections because they hold a water drop in place, not allowing it to spread out as other adhesion slides do. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From carl.hobbs at kcl.ac.uk Sat Jan 9 12:40:04 2021 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sat, 9 Jan 2021 18:40:04 +0000 Subject: [Histonet] Pre-made chrome alum slides source? Message-ID: Hi I know that you are well aware that you can make your own, easily. (I agree re the hydrophobicity induced) I am interested to know why you do not wish to, Tim I have to admit I don't make silanised slides anymore...I buy Superfrost Plus, for example. Shame on me ....home-silanised are equally good, imho. Interested in differing opinions.... Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From Nancy.Schmitt at mercyhealth.com Mon Jan 11 10:18:28 2021 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Mon, 11 Jan 2021 16:18:28 +0000 Subject: [Histonet] CBG recycling Message-ID: Hello- We have been happily and successfully using the CBG recyclers for formalin and solvent recycling for many years. In the last three months, we have been seeing failure with the Reagent #2 for the formalin testing- it should be good for 3 months after opening, but we are seeing failure consistently after two weeks; wasting a LOT of reagent. Company has been VERY difficult to in touch with and isn't being too helpful......... Anybody else having issue with this? Thank You! Nancy Schmitt HT, MLT(ASCP) Pathology Support Services MercyOne Dubuque Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From sandra.cheasty at wisc.edu Tue Jan 12 12:41:00 2021 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Tue, 12 Jan 2021 18:41:00 +0000 Subject: [Histonet] Block Holder for Leica Rm2255 Message-ID: Hello all, The handle snapped off our RM2255 microtome block holder, and the replacement we were sent does not open the clamp far enough. (We are currently managing by using an allen wrench in the drilled out hole...) Does anyone have any suggestions? Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From Kelly.Pairan at nationwidechildrens.org Wed Jan 13 22:40:49 2021 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Thu, 14 Jan 2021 04:40:49 +0000 Subject: [Histonet] Wilm Tumor Carboxy Terminus Message-ID: Good Evening Histoland, We currently offer Wilms Tumor carboxy-terminus IHC on FFPE at our institution. Our current supplier has had a backorder that has changed dates multiple times. If your institution runs this antibody, I would really appreciate it if you would share your vendor with me. I'm having a hard time finding another supplier. Thanks for your help, Kelly Kelly Pairan, BS, HT (ASCP)CM, QIHC (ASCP) Histology Supervisor-Anatomic Pathology Department of Pathology and Laboratory Medicine Email: kelly.pairan at nationwidechildrens.org ph: 614-722-5414 fx: 614-722-3033 From relia1 at earthlink.net Thu Jan 14 11:48:22 2021 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 14 Jan 2021 12:48:22 -0500 Subject: [Histonet] Considering a Job Change? RELIA Solutions offers free career services to all histology professionals!! Happy NEW YEAR! Message-ID: <075801d6ea9d$73f3f060$5bdbd120$@earthlink.net> Hello Histopeeps, Wherever you want to go; whatever you want to do. If you are looking for a new opportunity I can help. I offer over 20 years of professional recruiting and a recruiting practice dedicated solely to permanent placement in the Histology profession. Introducing: RELIA's Career Coaching Program. I offer to you FREE of charge: * Assistance with updating or creating your resume * Tips on interviewing * Encouragement and Assistance during the course of your job search * Responsiveness - I will respond to your e-mails/phone calls within 24 hours or less. * Troubleshooting - if your job search is stalled or you can't get in the company you are interested in. * Personalized Job Search - customized to your experience, wants and needs. * Complete Confidentiality - Your career and my coaching is a relationship of trust. I have a nationwide network of contacts with the premier employers of histology professionals in clinical, research and biotech settings. I only work with full time permanent positions at companies that offer excellent compensation and benefits programs. Here is where my most exciting opportunities are located: . Florida . Texas . California . North Carolina . New York If you or any of your friends would like more information on RELIA'S Career Coaching or about any of the positions listed please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or relia1 at earthlink.net Remember it never hurts to look. Hope to hear from you soon. Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From adesupo2002 at hotmail.com Sun Jan 17 21:39:37 2021 From: adesupo2002 at hotmail.com (ADESUPO ADESUYI) Date: Mon, 18 Jan 2021 03:39:37 +0000 Subject: [Histonet] Standards/Benchmarks Message-ID: Hi, Does anyone have an idea of how many blocks a tech can embed/cut/gross per hour according to the ASCP/NSH Standards? Thanks, Adesupo Adesuyi From akemiat3377 at gmail.com Tue Jan 19 17:08:04 2021 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Tue, 19 Jan 2021 16:08:04 -0700 Subject: [Histonet] GLP lot# documentation Message-ID: Hello Histo Land: I am the new IHC manager for a research lab which works primarily with animal tissue and we are moving towards GLP compliance for our department. We have an extremely large client base which consists of Big Pharma, private research companies, universities, etc? We are in the process of updating our SOW and SOP forms, Stain line maintenance forms, solution stainer change logs, etc? I have a question regarding documenting lot #?s for our hematoxylin counterstain protocol for IHC's. Do any of you who are GLP compliant document lot numbers for your alcohols and xylenes? Our director and QA officer would like to incorporate documenting lot numbers for alcohols and xylenes. Our histology department who is GLP certified only documents their lot numbers for Hematoxylin, Eosin, Bluing solution and High Def solutions for their H&E stain line. They currently do not document lot numbers for alcohols and xylenes. I have primarily worked for Clinical pathology labs, who did not document lot numbers for alcohols or xylenes. Please let me know if you do. Thanks, Akemi Eileen (Akemi) Allison, BS, HT/HTL (ASCP) Manager ? IHC Department HistoTox Labs, Inc. 2108 55th St. Suite 120 Boulder, CO 80301 Cell: 408-335-9994 www.histotoxlabs.com From relia1 at earthlink.net Wed Jan 20 10:35:09 2021 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 20 Jan 2021 11:35:09 -0500 Subject: [Histonet] ICYMI Here's my latest article - The Importance of Mentorship and some exciting job opportunities in FL, CA, TX, NC, NY and GA!! Message-ID: <000001d6ef4a$384ae8a0$a8e0b9e0$@earthlink.net> Hi Histopeeps, ICYMI - In case you missed it!! I wanted to let you know my latest article has been published in the NSH Quarterly Career Center Newsletter under my byline: Make the Cut My latest article is entitled: "The Importance of Mentorship". I was inspired to write about this after realizing how many titans of histology we have lost recently. This is the link to my article on the NSH's Fixation on Histology Blog and it is also in the current edition of the NSH Career Newsletter. Here is the link to the article: https://www.fixationonhistology.com/post/the-importance-of-mentorship If you have a minute to read it I would love to hear what you think. If you can't get to the article with this link let me know and I will send you a copy of it. Histopeeps, we also have amazing opportunities nationwide in! Texas California Florida Georgia Tennessee New York North Carolina With new opportunities coming in from all over the U.S. daily. If you don't see your location PLEASE let me know. I might have the perfect position for you and might come in BEFORE the next newsletter!! Please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral reward! I LOVE TO GIVE REFERRAL REWARDS!! Several of these positions are RELIA exclusives! You won't see them anyplace else! My clients offer competitive pay rates, excellent benefits and in most cases Relocation assistance or a sign on bonus. They are ready to interview and hire!!! What more could you ask for? If You or Anyone You Know Might Be Interested In a New Opportunity, Please Contact Me ASAP If you want to chat ASAP call or text me on my cell At 407-353-5070. If you want some additional information or to set up a time to chat please call me toll free at 866-607-3542 or email me at relia1 at earthlink.net My clients are ready to interview and hire right away!!! And my phone is still ringing off the hook so if you don't see the location you want yet drop me a line so I can let you know when something DOES come up. I could be talking to a client about your next opportunity RIGHT NOW!! Have a great day. I look forward to hearing back from you. Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From cmcgrad1 at hurleymc.com Thu Jan 21 12:21:14 2021 From: cmcgrad1 at hurleymc.com (Cynthia McGrady) Date: Thu, 21 Jan 2021 13:21:14 -0500 Subject: [Histonet] H Pylori controls Message-ID: Hello Histonetters. Looking for positive H Pylori control block(s). We typically use positive case tissue from old surgical cases, but seem to be running low. If anyone has a surplus, and would be willing to share, or maybe we have tissue you can use?? We could do a swap..Let me know..Thanks...Cindy McGrady Cindy McGrady HT(ASCP) QIHC Hurley Medical Center One Hurley Plaza Flint, Mi 48503 810-262-9138 -- If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. From vollecra at gmail.com Thu Jan 21 12:35:25 2021 From: vollecra at gmail.com (Craig) Date: Thu, 21 Jan 2021 12:35:25 -0600 Subject: [Histonet] Service provider for growing fungal strains? Message-ID: Hello all! Does anyone know a lab that can grow specific fungal strains? We are looking to make internal FFPE control blocks for specific fungal strains. We have an internal method for producing FFPE blocks. Just need a lab or service provider that can grow specific fungal cultures and send us the fixed bugs. Any suggestions would super grateful! Best, Craig vollecra at gmail.com craig at champdx.com ? From thisisann at aol.com Fri Jan 22 09:43:26 2021 From: thisisann at aol.com (Ann Specian) Date: Fri, 22 Jan 2021 15:43:26 +0000 (UTC) Subject: [Histonet] Embedding References: <655838156.2928090.1611330206544.ref@mail.yahoo.com> Message-ID: <655838156.2928090.1611330206544@mail.yahoo.com> Is anyone aware of any NYS/CAP/CLIA regulations regarding credentialing required to perform embedding in NJ?Thank you, Ann From b-frederick at northwestern.edu Fri Jan 22 11:15:40 2021 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Fri, 22 Jan 2021 17:15:40 +0000 Subject: [Histonet] water under sectios Message-ID: Good morning (well, in Chicago anyway) We've recently been having issues with water under the sections when picked up from the waterbath. It seems to even affect the sections during antigen retrieval after removing excess water, drying and baking I was given a block from an outside source that I know is a different paraffin and there was no water to be seen. Same person,same techniques. Can paraffin play a part? Or is the processing throwing this off. We run various programs and it seems to happen with almost everything. TMA"s in particular, as cores get lost during retrieval. Bernice Bernice Frederick Pathology Core Facility Robert H. Lurie Cancer Center 710 North Fairbanks Court Olson 8-421 312-503-3723 b-frederick at northwestern.edu From greg.dobbin at gmail.com Fri Jan 22 12:45:34 2021 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Fri, 22 Jan 2021 14:45:34 -0400 Subject: [Histonet] Water under sections Message-ID: Hi Bernice, In my lab, water under the sections is unique to charged slides. And you are correct, if there is water under the section when the slides are heated for antigen retrieval, the boiling (or at least very hot) water will damage or entirely destroy the section. We allow the charged slides to drain (upright) for a few minutes and then carefully make a hole in the edge of the wax and use a Kimwipe or paper towel to carefully wick the excess water out as much as we can without touching the tissue section. Then we "flick" or shake sharply to remove any residual water that may remain before we bake them (FYI, we choose not to bake on the stainer). If after baking 30 mins at 60C, we notice water (maybe someone was not as diligent earlier?), we wick away the excess and bake for another 15 mins to ensure good adhesion of the section to the slide before proceeding to the immunostainer. Hope this helps. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From ASelf at tidelandshealth.org Fri Jan 22 14:05:39 2021 From: ASelf at tidelandshealth.org (Amy Self) Date: Fri, 22 Jan 2021 20:05:39 +0000 Subject: [Histonet] Order Test in Histology Message-ID: Happy Friday Everyone, How do your pathologist get orders to the histology department? Are they sent manual(pencil/paper) - electronic through the case - email? Also how do you receive orders from outside facilities? We currently receive our orders via email or fax. This process needs to be improved. Do pathology reports serve as a signed order for pathologist? Thanks in advance and I hope that everyone stays safe and has a great weekend. Amy Self Senior Histology Technologist Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 Office: (843) 520-8711 aself at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From Timothy.Morken at ucsf.edu Fri Jan 22 14:15:29 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 22 Jan 2021 20:15:29 +0000 Subject: [Histonet] Order Test in Histology In-Reply-To: References: Message-ID: Amy, all our orders are thru our LIS, Copath Plus. Residents or pathologist order on their own. Except for grossed cases. They get standard panels ordered upon accession or grossing. Outside cases are all consults. Some are routine (for instance our kidney lab where we do routine panels) so they are ordered on accession. Others are custom order per pathologist review. I'm not sure what you mean by whether a "pathology report serves as a signed order for pathologist." Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Amy Self via Histonet Sent: Friday, January 22, 2021 12:06 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Order Test in Histology Happy Friday Everyone, How do your pathologist get orders to the histology department? Are they sent manual(pencil/paper) - electronic through the case - email? Also how do you receive orders from outside facilities? We currently receive our orders via email or fax. This process needs to be improved. Do pathology reports serve as a signed order for pathologist? Thanks in advance and I hope that everyone stays safe and has a great weekend. Amy Self Senior Histology Technologist Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 Office: (843) 520-8711 aself at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=cGJYu3PO9kirz17mYDEBkw_UkuQ1vkrzTAaCtRXlZ2k&s=VFpVgPkbcsfI6eanqSHDquA7ercKv8XuAAC1jZwmm-w&e= From Hans at histologistics.com Fri Jan 22 14:50:28 2021 From: Hans at histologistics.com (Hans B Snyder) Date: Fri, 22 Jan 2021 15:50:28 -0500 Subject: [Histonet] H Pylori controls In-Reply-To: References: Message-ID: Hello Cynthia, We have HP blocks. Our tissues are great for IHC but not special stains. Please contact me directly if you are interested. Thank you Hans B Snyder JMD Histology & Histologistics Inc. 118 Main St, Store Front, Suite 1 Webster, MA 01570 Lab - 508-461-7207 www.histologistics.com http://jmdhistology.com/ -------------------------------------To Err is Human----------------------------------- The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. On Fri, Jan 22, 2021 at 1:12 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. H Pylori controls (Cynthia McGrady) > 2. Service provider for growing fungal strains? (Craig) > 3. Embedding (Ann Specian) > 4. water under sectios (Bernice Frederick) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 21 Jan 2021 13:21:14 -0500 > From: Cynthia McGrady > To: Histonet > Subject: [Histonet] H Pylori controls > Message-ID: > < > CAFn2HSHEzLsrD-8brzJ+8eZkNN3zxVogLZ93+bgmoV820sz07w at mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hello Histonetters. > Looking for positive H Pylori control block(s). We typically use > positive case tissue from old surgical cases, but seem to be running low. > If anyone has a surplus, and would be willing to share, or maybe we have > tissue you can use?? We could do a swap..Let me know..Thanks...Cindy > McGrady > > Cindy McGrady > HT(ASCP) QIHC > Hurley Medical Center > One Hurley Plaza > Flint, Mi 48503 > 810-262-9138 > > -- > > > If you are not the intended > recipient(s), you are notified that any > disclosure, copying, distribution or > any action taken or omitted to be > taken in reliance on the contents of this > information is prohibited and may > be unlawful. If you receive this message in > error, or are not the named > recipient(s), please notify the sender, delete this > e-mail from your > computer, and destroy any copies in any form immediately. > > > ------------------------------ > > Message: 2 > Date: Thu, 21 Jan 2021 12:35:25 -0600 > From: Craig > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Service provider for growing fungal strains? > Message-ID: > 9Lyz12AXOa67MV0CDFak1V0n5cmXNmFYU90VYDFe7kkA at mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hello all! > > Does anyone know a lab that can grow specific fungal strains? > > We are looking to make internal FFPE control blocks for specific fungal > strains. We have an internal method for producing FFPE blocks. Just need a > lab or service provider that can grow specific fungal cultures and send us > the fixed bugs. > > Any suggestions would super grateful! > > Best, > Craig > vollecra at gmail.com > craig at champdx.com > ? > > > ------------------------------ > > Message: 3 > Date: Fri, 22 Jan 2021 15:43:26 +0000 (UTC) > From: Ann Specian > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Embedding > Message-ID: <655838156.2928090.1611330206544 at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > Is anyone aware of any NYS/CAP/CLIA regulations regarding credentialing > required to perform embedding in NJ?Thank you, Ann > > ------------------------------ > > Message: 4 > Date: Fri, 22 Jan 2021 17:15:40 +0000 > From: Bernice Frederick > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] water under sectios > Message-ID: > < > MWHPR05MB294386DEF0662AF11F9FF66C8FA09 at MWHPR05MB2943.namprd05.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > Good morning (well, in Chicago anyway) > We've recently been having issues with water under the sections when > picked up from the waterbath. It seems to even affect the sections during > antigen retrieval after removing excess water, drying and baking I was > given a block from an outside source that I know is a different paraffin > and there was no water to be seen. Same person,same techniques. Can > paraffin play a part? Or is the processing throwing this off. We run > various programs and it seems to happen with almost everything. TMA"s in > particular, as cores get lost during retrieval. > Bernice > > Bernice Frederick > Pathology Core Facility > Robert H. Lurie Cancer Center > 710 North Fairbanks Court > Olson 8-421 > 312-503-3723 > b-frederick at northwestern.edu > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 206, Issue 9 > **************************************** > From mwerdler at gmail.com Fri Jan 22 14:58:36 2021 From: mwerdler at gmail.com (Mca Werdler) Date: Fri, 22 Jan 2021 21:58:36 +0100 Subject: [Histonet] H Pylori controls In-Reply-To: References: Message-ID: Unsubscribe Op vr 22 jan. 2021 9:53 p.m. schreef Hans B Snyder via Histonet < histonet at lists.utsouthwestern.edu>: > Hello Cynthia, > > We have HP blocks. Our tissues are great for IHC but not special stains. > Please contact me directly if you are interested. > > Thank you > > Hans B Snyder > JMD Histology & Histologistics Inc. > 118 Main St, Store Front, Suite 1 > Webster, MA 01570 > Lab - 508-461-7207 > www.histologistics.com > http://jmdhistology.com/ > -------------------------------------To Err is > Human----------------------------------- > > The information contained in this message and any attachments is intended > only > for the use of the individual or entity to which it is addressed, and may > contain > information that is privileged, confidential and exempt from disclosure > under > applicable law. If you have received this message in error, you are > prohibited > from copying, distributing or using the information. Please contact the > sender > immediately by return e-mail and delete the original message. > > > On Fri, Jan 22, 2021 at 1:12 PM > > wrote: > > > Send Histonet mailing list submissions to > > histonet at lists.utsouthwestern.edu > > > > To subscribe or unsubscribe via the World Wide Web, visit > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > or, via email, send a message with subject or body 'help' to > > histonet-request at lists.utsouthwestern.edu > > > > You can reach the person managing the list at > > histonet-owner at lists.utsouthwestern.edu > > > > When replying, please edit your Subject line so it is more specific > > than "Re: Contents of Histonet digest..." > > > > > > Today's Topics: > > > > 1. H Pylori controls (Cynthia McGrady) > > 2. Service provider for growing fungal strains? (Craig) > > 3. Embedding (Ann Specian) > > 4. water under sectios (Bernice Frederick) > > > > > > ---------------------------------------------------------------------- > > > > Message: 1 > > Date: Thu, 21 Jan 2021 13:21:14 -0500 > > From: Cynthia McGrady > > To: Histonet > > Subject: [Histonet] H Pylori controls > > Message-ID: > > < > > CAFn2HSHEzLsrD-8brzJ+8eZkNN3zxVogLZ93+bgmoV820sz07w at mail.gmail.com> > > Content-Type: text/plain; charset="UTF-8" > > > > Hello Histonetters. > > Looking for positive H Pylori control block(s). We typically use > > positive case tissue from old surgical cases, but seem to be running low. > > If anyone has a surplus, and would be willing to share, or maybe we have > > tissue you can use?? We could do a swap..Let me know..Thanks...Cindy > > McGrady > > > > Cindy McGrady > > HT(ASCP) QIHC > > Hurley Medical Center > > One Hurley Plaza > > Flint, Mi 48503 > > 810-262-9138 > > > > -- > > > > > > If you are not the intended > > recipient(s), you are notified that any > > disclosure, copying, distribution or > > any action taken or omitted to be > > taken in reliance on the contents of this > > information is prohibited and may > > be unlawful. If you receive this message in > > error, or are not the named > > recipient(s), please notify the sender, delete this > > e-mail from your > > computer, and destroy any copies in any form immediately. > > > > > > ------------------------------ > > > > Message: 2 > > Date: Thu, 21 Jan 2021 12:35:25 -0600 > > From: Craig > > To: histonet at lists.utsouthwestern.edu > > Subject: [Histonet] Service provider for growing fungal strains? > > Message-ID: > > > 9Lyz12AXOa67MV0CDFak1V0n5cmXNmFYU90VYDFe7kkA at mail.gmail.com> > > Content-Type: text/plain; charset="UTF-8" > > > > Hello all! > > > > Does anyone know a lab that can grow specific fungal strains? > > > > We are looking to make internal FFPE control blocks for specific fungal > > strains. We have an internal method for producing FFPE blocks. Just need > a > > lab or service provider that can grow specific fungal cultures and send > us > > the fixed bugs. > > > > Any suggestions would super grateful! > > > > Best, > > Craig > > vollecra at gmail.com > > craig at champdx.com > > ? > > > > > > ------------------------------ > > > > Message: 3 > > Date: Fri, 22 Jan 2021 15:43:26 +0000 (UTC) > > From: Ann Specian > > To: "histonet at lists.utsouthwestern.edu" > > > > Subject: [Histonet] Embedding > > Message-ID: <655838156.2928090.1611330206544 at mail.yahoo.com> > > Content-Type: text/plain; charset=UTF-8 > > > > Is anyone aware of any NYS/CAP/CLIA regulations regarding credentialing > > required to perform embedding in NJ?Thank you, Ann > > > > ------------------------------ > > > > Message: 4 > > Date: Fri, 22 Jan 2021 17:15:40 +0000 > > From: Bernice Frederick > > To: "histonet at lists.utsouthwestern.edu" > > > > Subject: [Histonet] water under sectios > > Message-ID: > > < > > > MWHPR05MB294386DEF0662AF11F9FF66C8FA09 at MWHPR05MB2943.namprd05.prod.outlook.com > > > > > > > Content-Type: text/plain; charset="us-ascii" > > > > Good morning (well, in Chicago anyway) > > We've recently been having issues with water under the sections when > > picked up from the waterbath. It seems to even affect the sections during > > antigen retrieval after removing excess water, drying and baking I was > > given a block from an outside source that I know is a different paraffin > > and there was no water to be seen. Same person,same techniques. Can > > paraffin play a part? Or is the processing throwing this off. We run > > various programs and it seems to happen with almost everything. TMA"s in > > particular, as cores get lost during retrieval. > > Bernice > > > > Bernice Frederick > > Pathology Core Facility > > Robert H. Lurie Cancer Center > > 710 North Fairbanks Court > > Olson 8-421 > > 312-503-3723 > > b-frederick at northwestern.edu > > > > > > > > ------------------------------ > > > > Subject: Digest Footer > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > > > End of Histonet Digest, Vol 206, Issue 9 > > **************************************** > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pruegghm at hotmail.com Sat Jan 23 18:22:57 2021 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Sun, 24 Jan 2021 00:22:57 +0000 Subject: [Histonet] Water under sections In-Reply-To: References: Message-ID: This is all good advise. I always picked up section and tapped the slide on counter and then dried standing up. This is really a problem if you are at all above 5K feet in altitude where water boils violently at 92-93dc, which will destroy your tissue section. I have written a couple of papers about on this. ________________________________ From: Greg Dobbin Sent: Friday, January 22, 2021 11:45 AM To: b-frederick at northwestern.edu Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Water under sections Hi Bernice, In my lab, water under the sections is unique to charged slides. And you are correct, if there is water under the section when the slides are heated for antigen retrieval, the boiling (or at least very hot) water will damage or entirely destroy the section. We allow the charged slides to drain (upright) for a few minutes and then carefully make a hole in the edge of the wax and use a Kimwipe or paper towel to carefully wick the excess water out as much as we can without touching the tissue section. Then we "flick" or shake sharply to remove any residual water that may remain before we bake them (FYI, we choose not to bake on the stainer). If after baking 30 mins at 60C, we notice water (maybe someone was not as diligent earlier?), we wick away the excess and bake for another 15 mins to ensure good adhesion of the section to the slide before proceeding to the immunostainer. Hope this helps. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From andrea at ka-recruiting.com Sun Jan 24 20:06:56 2021 From: andrea at ka-recruiting.com (Andrea Costello) Date: Sun, 24 Jan 2021 21:06:56 -0500 Subject: [Histonet] Histology Positions from Bench to Management from Florida to Nevada! Message-ID: Hello Histonetters! Happy Monday! Over the past month, we have gotten in more *high priority* histology jobs than we've had in the past year! Each of these positions is *permanent and full time* and our clients are extremely motivated to hire are all offering highly competitive compensation packages (most include sign on or relocation)! If you are interested in learning more OR if you are looking for a change feel free to reach out to me directly at andrea at ka-recruiting.com or call/text 6177462745! AZ - HIstology Supervisor FL - Histology Manager GA - Histotech - Early Days (either 2am or 3am start time) GA - Histology Supervisor - Nights ND - Histology Supervisor NV - Histotechnologist - Grossing (HTL Required) NY - Histology Supervisor - Evenings NY - Grossing Supervisor NY - Histotech - Day, Evening or Nights NY - Grossing Tech - Night Shift TN - Grossing/ Histology Supervisor - Nights TN- Histotech - Nights VA - Histotech (Afternoon/Evening Shift) WI - Histotech - Evenings or Nights - Possibility of Days Andrea Costello Associate Vice President Client Relationship Manager K.A. Recruiting, Inc. 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 *P: (617) 746-2745* *F: (617) 507-8009 * *E: andrea at ka-recruiting.com * From tcassidy2511 at gmail.com Mon Jan 25 10:51:24 2021 From: tcassidy2511 at gmail.com (Teri Cassidy) Date: Mon, 25 Jan 2021 11:51:24 -0500 Subject: [Histonet] BMKSS/Jones Message-ID: Reaching out to see if anyone else is having a problem with the Jones testing from Roche? We have had numerous failed stains since October with no real resolve. Some days it works some days it fails. Machines deconned and wash made correctly. All probable issues have been looked into and solved. At this point running the slides manually. Any help would be appreciated. Teri. From tgenade at protonmail.com Mon Jan 25 11:25:04 2021 From: tgenade at protonmail.com (Tyrone Genade) Date: Mon, 25 Jan 2021 17:25:04 +0000 Subject: [Histonet] Water under sections Message-ID: <0dXfWm3uSYDtE5qP8Zpi6QWytGlvYZIEBqaFu0Zd-4BNnCqHjdQx7rG02r0hIsUu__xxGgVQ38s3XrF8MiX80uZJCdLTjE4PS8Pq77nCmZo=@protonmail.com> Hello, On Saturday, January 23, 2021 1:00 PM, wrote: > In my lab, water under the sections is unique to charged slides. > And you > are correct, if there is water under the section when the slides > are heated > for antigen retrieval, the boiling (or at least very hot) water > will damage > or entirely destroy the section. I had this very problem and the simple solution was to bake the slides overnight at 56oC (melting temp of the wax I was working with). I would still heat on a hot plate before dewaxing. Baking at 45oC also seemed to work. Whether this would work for you would depend on the sensitivity of your epitopes to prolonged heat. Good luck... every now and then a section would still detach during antigen retrieval. From cforster at umn.edu Mon Jan 25 12:33:16 2021 From: cforster at umn.edu (Colleen Forster) Date: Mon, 25 Jan 2021 12:33:16 -0600 Subject: [Histonet] Water under sections In-Reply-To: <0dXfWm3uSYDtE5qP8Zpi6QWytGlvYZIEBqaFu0Zd-4BNnCqHjdQx7rG02r0hIsUu__xxGgVQ38s3XrF8MiX80uZJCdLTjE4PS8Pq77nCmZo=@protonmail.com> References: <0dXfWm3uSYDtE5qP8Zpi6QWytGlvYZIEBqaFu0Zd-4BNnCqHjdQx7rG02r0hIsUu__xxGgVQ38s3XrF8MiX80uZJCdLTjE4PS8Pq77nCmZo=@protonmail.com> Message-ID: I use charged slides all the time. The best way to clear the water is to cut the slides and air dry them overnight. The next day I follow the normal protocol, heat in 60 degree over and then on to the rest of the protocol. They don't really need the 56 degree oven all night long. Respectfully, Colleen Forster On Mon, Jan 25, 2021 at 11:26 AM Tyrone Genade via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello, > > > On Saturday, January 23, 2021 1:00 PM, < > histonet-request at lists.utsouthwestern.edu> wrote: > > > In my lab, water under the sections is unique to charged slides. > > And you > > are correct, if there is water under the section when the slides > > are heated > > for antigen retrieval, the boiling (or at least very hot) water > > will damage > > or entirely destroy the section. > > I had this very problem and the simple solution was to bake the slides > overnight at 56oC (melting temp of the wax I was working with). I would > still heat on a hot plate before dewaxing. Baking at 45oC also seemed to > work. Whether this would work for you would depend on the sensitivity of > your epitopes to prolonged heat. > > Good luck... every now and then a section would still detach during > antigen retrieval. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 612-626-1930 From Melissa.Kuhnla at chsli.org Tue Jan 26 12:08:18 2021 From: Melissa.Kuhnla at chsli.org (Kuhnla, Melissa) Date: Tue, 26 Jan 2021 18:08:18 +0000 Subject: [Histonet] paraffin block disposal Message-ID: <6ec7f8d26bb849f08feccdd00dc65d0e@chsli.org> Good Afternoon All, Asking for a colleague,. How are sites disposing of old formalin fixed paraffin embedded surgical blocks? Garbage? Red bag? Thank you :) Melissa Kuhnla Lead Medical Technologist for IHC and FISH testing Regional Laboratory Services Good Samaritan Hospital 631-609-2551 From wilfong1923 at gmail.com Wed Jan 27 12:09:32 2021 From: wilfong1923 at gmail.com (John Frazier) Date: Wed, 27 Jan 2021 13:09:32 -0500 Subject: [Histonet] Histonet Digest, Vol 206, Issue 14 In-Reply-To: References: Message-ID: Remember to consider HIPPA if there are any potential identifiers on the blocks. *John Frazier* *MT(ASCP), MBA* *Lean 6 Six Sigma Black Belt* *Healthcare Consultant* *C - 302-310-7567* *H - 704-847-0566* *wilfong1923 at gmail.com * On Wed, Jan 27, 2021 at 1:00 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. paraffin block disposal (Kuhnla, Melissa) > > > > ---------- Forwarded message ---------- > From: "Kuhnla, Melissa" > To: "'histonet at lists.utsouthwestern.edu'" < > histonet at lists.utsouthwestern.edu> > Cc: > Bcc: > Date: Tue, 26 Jan 2021 18:08:18 +0000 > Subject: [Histonet] paraffin block disposal > Good Afternoon All, > Asking for a colleague,. How are sites disposing of old formalin fixed > paraffin embedded surgical blocks? Garbage? Red bag? > > Thank you :) > > Melissa Kuhnla > Lead Medical Technologist > for IHC and FISH testing > Regional Laboratory Services > Good Samaritan Hospital > 631-609-2551 > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dawn.Olszewski at SGMC.ORG Wed Jan 27 13:45:10 2021 From: Dawn.Olszewski at SGMC.ORG (Olszewski, Dawn) Date: Wed, 27 Jan 2021 19:45:10 +0000 Subject: [Histonet] Histonet Digest, Vol 206, Issue 14 In-Reply-To: References: <12a3486f-aba0-466f-9fef-6a4ca11e0a30.3b5c5640-2195-4f35-b5ef-5e1ebee68512.fd8c1d6a-97da-40ae-862e-5f545251eeaa@emailsignatures365.codetwo.com> Message-ID: We use the red bag method after we pull out any we want to use as controls and after Oncology pulls out any they want for Tumor bank. Dawn Olszewski Pathology Manager - Histotechnologist Laboratory [cid:MasterLogo_190x61_362365a5-c941-4f16-9f99-9484fd6a6078.png] South Georgia Medical Center 2501 N. Patterson St. Valdosta, GA 31602 229-259-4830 (O) | 229-560-6191 (M) Dawn.Olszewski at SGMC.ORG | sgmc.org [cid:facebook_fb_32x32_0a6d9065-09e0-48f7-88c4-b2d459a5e547.png] [cid:twitter_32x32_5a3a5eeb-77c7-4a3c-aa09-b69b41f32bd2.png] [cid:linkedin_ln_32x32_bf1e5dc9-b886-480a-8c8b-ef8a1ae87168.png] [cid:youtube_play_32x32_572cd3ed-390b-48e0-8845-ba1f81953de3.png] ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Wednesday, January 27, 2021 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 206, Issue 14 WARNING: This email originated outside the SGMC?s email system. DO NOT CLICK: Links or attachments unless you recognize the sender and know the content is safe. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7CDawn.Olszewski%40sgmc.org%7C02acc8fc1203481a919408d8c2ed764c%7C90cc13a5f2044538be2d7055be683ef1%7C1%7C0%7C637473672470221210%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=luwIoTCgmuVkhi97VhE%2FGteVohDzo82CiIZOCRyoxKo%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. paraffin block disposal (Kuhnla, Melissa) ---------------------------------------------------------------------- Message: 1 Date: Tue, 26 Jan 2021 18:08:18 +0000 From: "Kuhnla, Melissa" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] paraffin block disposal Message-ID: <6ec7f8d26bb849f08feccdd00dc65d0e at chsli.org> Content-Type: text/plain; charset="us-ascii" Good Afternoon All, Asking for a colleague,. How are sites disposing of old formalin fixed paraffin embedded surgical blocks? Garbage? Red bag? Thank you :) Melissa Kuhnla Lead Medical Technologist for IHC and FISH testing Regional Laboratory Services Good Samaritan Hospital 631-609-2551 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7CDawn.Olszewski%40sgmc.org%7C02acc8fc1203481a919408d8c2ed764c%7C90cc13a5f2044538be2d7055be683ef1%7C1%7C0%7C637473672470221210%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=luwIoTCgmuVkhi97VhE%2FGteVohDzo82CiIZOCRyoxKo%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 206, Issue 14 ***************************************** From robrankin at rankinbiomed.com Wed Jan 27 00:42:24 2021 From: robrankin at rankinbiomed.com (Rob Rankin) Date: Wed, 27 Jan 2021 01:42:24 -0500 Subject: [Histonet] Veterinary Histopathology Lab Message-ID: The owner of a histopathology lab in Puerto Rico is seriously considering starting a veterinary histology lab and wants to know if there are any differences from a human histology lab. Any feedback is appreciated, including referring someone that may know. Respectfully, *Rob Rankin, MSM, SM(ASCP)* Founder & CEO Office: 248 625-4104 ext 0012 www.rankinbiomed.com 14515 Mackey Rd. Holly, MI 48442 *Have not I commanded thee? Be strong and of good courage; be not afraid, neither be thou dismayed: for the LORD thy God is with thee whithersoever thou goest. * *Joshua 1:9* From Christopher.Hagon at act.gov.au Wed Jan 27 21:20:05 2021 From: Christopher.Hagon at act.gov.au (Hagon, Christopher (Health)) Date: Thu, 28 Jan 2021 03:20:05 +0000 Subject: [Histonet] Tissue Tek AutoTEC a120 Message-ID: OFFICIAL Hello Histonet! Just wondering if anyone has had experience or comments regarding the TissueTek AutoTEC a120? We are looking into a new embedding station and it came up as a possibility. Kind regards, Chris Hagon | Senior Scientist, Anatomical Pathology ACT Pathology ----------------------------------------------------------------------- This email, and any attachments, may be confidential and also privileged. If you are not the intended recipient, please notify the sender and delete all copies of this transmission along with any attachments immediately. You should not copy or use it for any purpose, nor disclose its contents to any other person. ----------------------------------------------------------------------- From emartinez2 at echd.org Thu Jan 28 09:53:30 2021 From: emartinez2 at echd.org (Estela Martinez) Date: Thu, 28 Jan 2021 15:53:30 +0000 Subject: [Histonet] Dipp Kwik Stain Message-ID: Hello All, Do any of your pathologist use Dipp Kwik and if so, what kind of control do you use and do you document the control? TIA! Estela Martinez Histology Supervisor Medical Center Hospital Odessa, TX 79761 432-640-2348 emartinez2 at echd.org CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party without permission of original user and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From tony.henwood at health.nsw.gov.au Thu Jan 28 14:31:19 2021 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 28 Jan 2021 20:31:19 +0000 Subject: [Histonet] Dipp Kwik Stain In-Reply-To: References: Message-ID: <1611865882337.15518@health.nsw.gov.au> There is a generally accepted scheme of staining which is expected of Romanowsky stained preparations, namely purple chromatin, blue leucocyte cytoplasm, purple-black basophil granules, red-pink eosinophil granules, purple neutrophil granules, purple platelet granules, and pink red-cells (1). According to recommendations of the French Society of Clinical Cytology and of the French Association for Quality Assurance in Anatomic and Cytologic Pathology (2), red blood cells and polymorphonuclear leukocytes are the target cells for the quality evaluation of Romanowsky stains, and not the tumour cells. The tinctorial quality of Romanowsky stains is better and reliably judged on these target cells. Even if it is known that a ?good MGG? allows pink?orange (acidophilic) or buff-coloured RBCs to be obtained, many laboratories produce MGG slides showing blue or green erythrocytes, mainly because of a high (alkaline) pH of dilution/rinse solutions (3). Horobin (3) has provided a useful table to trouble-shoot Romanowsky staining. 1. Marshall PN, Bentley SA, Lewis SM. An evaluation of some commercial Romanowsky stains. Journal of clinical pathology. 1975 Aug 1;28(8):680-5. 2. Piaton E, Fabre M, Goubin?Versini I, Bretz?Grenier MF, Courtade?Sa?di M, Vincent S, Belleann?e G, Thivolet F, Boutonnat J, Debaque H, Fleury?Feith J. Guidelines for May?Gr?nwald?Giemsa staining in haematology and non?gynaecological cytopathology: recommendations of the French Society of Clinical Cytology (SFCC) and of the French Association for Quality Assurance in Anatomic and Cytologic Pathology (AFAQAP). Cytopathology. 2016 Oct;27(5):359-68. 3. Horobin RW. How Romanowsky stains work and why they remain valuable?including a proposed universal Romanowsky staining mechanism and a rational troubleshooting scheme. Biotechnic & Histochemistry. 2011 Feb 1;86(1):36-51. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Estela Martinez via Histonet Sent: Friday, 29 January 2021 02:53 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Dipp Kwik Stain Hello All, Do any of your pathologist use Dipp Kwik and if so, what kind of control do you use and do you document the control? TIA! Estela Martinez Histology Supervisor Medical Center Hospital Odessa, TX 79761 432-640-2348 emartinez2 at echd.org CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party without permission of original user and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From Timothy.Morken at ucsf.edu Fri Jan 29 13:55:21 2021 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 29 Jan 2021 19:55:21 +0000 Subject: [Histonet] Milestone Prestochill for muscle histochem Message-ID: Hi all, I was wondering if anyone has used the Milestone PrestoChill for freezing muscle for histochemistry. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From thisisann at aol.com Sat Jan 30 14:14:08 2021 From: thisisann at aol.com (Ann Specian) Date: Sat, 30 Jan 2021 20:14:08 +0000 (UTC) Subject: [Histonet] Hologic PAP Imaging References: <863988221.433371.1612037648922.ref@mail.yahoo.com> Message-ID: <863988221.433371.1612037648922@mail.yahoo.com> Is anyone using a xylene substitute when staining their PAPs and imaging using the Hologic Imagers?? If you are, can you tell me which xylene substitute you are using (as long as it is FDA approved).? Have you had any issues?? Thank you, Ann