From relia1 at earthlink.net Thu Apr 1 09:51:00 2021 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 1 Apr 2021 10:51:00 -0400 Subject: [Histonet] RELIA HOT Histology Opportunity Update! Brand New Lab and brand new opportunities!!! Message-ID: <005501d72706$6e7d2020$4b776060$@earthlink.net> Hello Histopeeps, I hope this has been an amazing week for you! Mine has been pretty great so far and I want to share it with you. I have some incredible new job opportunities. Most of them are RELIA exclusives: Super- HOT RELIA Exclusive Spotlight Opportunities: Bend, OR ? Histotechnician/Histotechnologist This is a full time day shift permanent position. You will be performing routine histology and IHC. ASCP certification is NOT required for this position. My client offers a very competitive pay rate, great benefits and relocation assistance. They have a great team to work with and are eager to have someone join their crew!! San Diego, CA ? Histologists ? BRAND NEW CLINICAL LAB!! That?s right!! A brand new lab opening soon and I have been engaged to staff it. These will be full time permanent positions in a brand new state of the art lab. If you are interested contact me ASAP for the details!! Histopeeps!! I also have amazing job opportunities in: ? California - Orange County and San Diego!! ? Oregon - Bend for you outdoorsy types!! ? Florida - Beachside on the SW Coast! ? Texas - Austin Funky and Fun! ? N. Carolina ?Fayetteville small town charm and Ft. Bragg. ? PA - Live and work at the foot of the mountains!! ? Tennessee - Chattanooga and Nashville!! ? Georgia - Atlanta HOTLanta #iykyk Most of these are RELIA Exclusives! That?s right you will only see them HERE! And New Opportunities are coming in on a daily basis!! All of these clients are offering full time permanent positions with excellent compensation packages including VERY competitive pay rates, fantastic benefits, relocation and/or sign on bonuses and a great team to work with!! I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. ? I can be reached toll free at 866-607-3542. 866-60RELIA ? Text or call me on my cell at 407-353-5070. ? Or email me at: relia1 at earthlink.net . Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From patpxs at gmail.com Mon Apr 5 12:36:54 2021 From: patpxs at gmail.com (P Sicurello) Date: Mon, 5 Apr 2021 10:36:54 -0700 Subject: [Histonet] UCSD Moore's Cancer Center needs a HIstotech Message-ID: Good Morning Listers, I have been asked by my lab manager to post this position. It appears they are having trouble finding a Histotech. Go to UCSD Title and Pay Information to find out the pay range. Here is the link: UC San Diego Jobs | search | 107387 (ucsd.edu) #107387 Histology Research Associate (Staff Research Associate II) Under direction of Pathologists, Lab Manager, and Lab Supervisor, the Histology Research Associate (HRA) will assist with clinical research and perform technical tasks in each lab section assigned. In the lab, they will work on the nanoString research platform (nCounter MAX, GeoMX/DSP) with human and mouse research tissues for multiplex tumor and immune profiling. May assist in PBMC, microbiome, and other specimen handling, processing, and clinical annotation. Maintains lab and performs lab inventory, ordering supplies, clerical, and other duties as directed. Prepares buffers and solutions as needed. Maintains laboratory environmental health and safety activities, including chemical and biological waste disposal. Coordinates development of tissue projects with lab manager, and coordinates associated activities. Will serve as the principal administrative liaison for the projects. Organizes and prepares specimens for distribution, and completes specimens in LIS, files slides and blocks, tracks specimens, maintains storage, retrieval and disposal of specimens per lab protocols. Under Pathology, they will conduct histological procedures in accordance with laboratory requirements. Duties include tissue accessioning, processing, embedding and staining. May assist with tissue accessioning & processing, embedding, microtomy and staining (routine and special), immunofluorescence, equipment preventive maintenance, quality control, slides cover-slipping, and delivers slides for pathologists. Assist with clinical research processing and clerical tasks. MINIMUM QUALIFICATIONS - Theoretical knowledge in biology, chemistry, histology and immunohistochemistry or a related field or an equivalent combination of education and experience. - Experience using balance, processor, embedder, and light and fluorescent microscopes. Comfortable and able to navigate new technology as the laboratory upgrades equipment. Ability to work on the NanoString research platform (nCounter MAX, GeoMx/DSP) with human and mouse research tissues for multiplex tumor and immune profiling. - Demonstrated experience cutting frozen and paraffin sections for histology. - Proven experience performing hematoxylin and eosin stains on paraffin or frozen sections. - Knowledge in histology, histochemistry and immunohistochemistry techniques. Experience performing immunohistochemistry assays after protocol review. - Experience performing special stains on paraffin or frozen sections, using adipose tissue as positive control. - Demonstrated experience calculating and preparing chemical solutions accurately. - Strong knowledge in basic math for maintaining ledgers, income and expenditures reports. - Proficient in the operation of a personal computer, including Access, MS Word and Excel. - Good communication skills (both verbal and written) to interact professionally and effectively in the work environment, as well as to effectively and accurately document research procedures. Ability to read, comprehend and discuss research materials. - Thorough knowledge of laboratory safety procedures involved in working with hazardous chemicals and appropriate use of Personal Protective Equipment. Ability to follow established Safety and Biohazardous Material Guidelines. - Experience to write Standard Operating Procedures (SOP) for using laboratory equipment following the manual instructions. Experience to instruct personnel with Standard Operating Procedures (SOP) for routine workflow processes. - Experience to train and instruct new staff, students assistant and volunteers with laboratory standards. Experience providing one-on-one training session for tissue grossing, tissue processing and tissue sectioning. - Demonstrated knowledge of chemical and biological waste disposal. - Skill and ability to keep accurate data. Ability to maintain confidentiality. Good organizational skills to order and maintain lab equipment and supplies. PREFERRED QUALIFICATIONS Histotechnician (HT)/Histotechnologist (HTL) Certification from American Society for Clinical Pathology (ASCP). Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From relia1 at earthlink.net Wed Apr 7 10:28:14 2021 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 7 Apr 2021 11:28:14 -0400 Subject: [Histonet] BRAND NEW LAB in San Diego!! A RELIA Exclusive!!! Certified and Uncertified techs wanted!!! Message-ID: <000701d72bc2$a06f56a0$e14e03e0$@earthlink.net> Hello Histopeeps, I hope you are having a great week. I am having a fantastic week! I am sending this quick note that could make your week fantastic too!! I have just been give an exciting new project and I want to share the details with you: This is a RELIA EXCLUSIVE you won?t see it anywhere else! This is a BRAND NEW LAB that is an arm of a well-known medical organization located in beautiful San Diego, CA! The lab is state of the art You get to work all stations in the lab Grossing is not required Positions are available for ASCP Certified and Uncertified Techs All Shifts Available All of these positions are full time and permanent!! My clients offer excellent compensation, benefits and relocation assistance. If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. ? If I place someone you refer You will earn a referral fee. ? If you refer someone to subscribe to my bulletins and I place them you will earn a referral fee. ? I love to give referral fees! If you are interested in these opportunities CALL /TEXT MY CELL ASAP!!! At 407-353-5070. I can also be reached toll free at the office at 866-607-3542 or at relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From tkngflght at yahoo.com Thu Apr 8 12:30:11 2021 From: tkngflght at yahoo.com (Cheryl) Date: Thu, 8 Apr 2021 17:30:11 +0000 (UTC) Subject: [Histonet] Is there a way to orient FFPE sections with an indicator that remains visible post staining? References: <1448590843.724181.1617903011238.ref@mail.yahoo.com> Message-ID: <1448590843.724181.1617903011238@mail.yahoo.com> Hi everyone- We have a researcher who needs to put multiple samples in one block for quality and cost control.? There are four different samples per tissue and they've been processed differently but all are FFPE.?? Is there a way to put an orientation mark in the block that transfers to the slide - like a section-able plastic bead or wax-based dye so we can embed these membranes in a specific order and she can keep track of the order on her future slide preps? I recall there was a cassette with little plastic 'fingers' to orient shave biopsies to embed later where the plastic cut and remained on the slide without interfering with any staining process or coverslipping- something like that?? Cheryl Kerry, HT(ASCP) Full Staff Inc. ? admin at fullstaff.orgtkgnflght@yahoo.com From cforster at umn.edu Thu Apr 8 12:50:01 2021 From: cforster at umn.edu (Colleen Forster) Date: Thu, 8 Apr 2021 12:50:01 -0500 Subject: [Histonet] Is there a way to orient FFPE sections with an indicator that remains visible post staining? In-Reply-To: <1448590843.724181.1617903011238@mail.yahoo.com> References: <1448590843.724181.1617903011238.ref@mail.yahoo.com> <1448590843.724181.1617903011238@mail.yahoo.com> Message-ID: Cheryl, When I have this I use a small cube of tissue that is completely unrelated to the project as my marker. You can even ink all surfaces on this small piece of tissue. I process something but don't block it and keep that piece to cut the smaller cubes from. I keep track on a "map" and then that goes with the block. It works well. Colleen Forster On Thu, Apr 8, 2021 at 12:30 PM Cheryl via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi everyone- > We have a researcher who needs to put multiple samples in one block for > quality and cost control. There are four different samples per tissue and > they've been processed differently but all are FFPE. > Is there a way to put an orientation mark in the block that transfers to > the slide - like a section-able plastic bead or wax-based dye so we can > embed these membranes in a specific order and she can keep track of the > order on her future slide preps? > I recall there was a cassette with little plastic 'fingers' to orient > shave biopsies to embed later where the plastic cut and remained on the > slide without interfering with any staining process or coverslipping- > something like that?? > > > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > > admin at fullstaff.orgtkgnflght@yahoo.com > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 612-626-1930 From erin.mccarthy at tempus.com Thu Apr 8 13:35:06 2021 From: erin.mccarthy at tempus.com (Erin McCarthy) Date: Thu, 8 Apr 2021 13:35:06 -0500 Subject: [Histonet] Is there a way to orient FFPE sections with an indicator that remains visible post staining? In-Reply-To: <1448590843.724181.1617903011238@mail.yahoo.com> References: <1448590843.724181.1617903011238.ref@mail.yahoo.com> <1448590843.724181.1617903011238@mail.yahoo.com> Message-ID: Hi Cheryl, I have heard of people using cucumber and processing it, but I have not actually tried it out myself! One of my R&D friends recommended it to me when trying to DIY some tumor microarrays. You might be able to try it out and see if it stains well - I have never actually seen it in person myself! On Thu, Apr 8, 2021 at 12:43 PM Cheryl via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi everyone- > We have a researcher who needs to put multiple samples in one block for > quality and cost control. There are four different samples per tissue and > they've been processed differently but all are FFPE. > Is there a way to put an orientation mark in the block that transfers to > the slide - like a section-able plastic bead or wax-based dye so we can > embed these membranes in a specific order and she can keep track of the > order on her future slide preps? > I recall there was a cassette with little plastic 'fingers' to orient > shave biopsies to embed later where the plastic cut and remained on the > slide without interfering with any staining process or coverslipping- > something like that?? > > > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > > admin at fullstaff.orgtkgnflght@yahoo.com > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Erin McCarthy, HT (ASCP) She/Her Histology Supervisor Tempus Labs 600 W. Chicago Ave. Chicago IL 60654 Cell: (708)269-8610 -- This email and any attachments may contain confidential information. If you believe that you have received it in error, please delete the email and attachments and then notify Tempus by calling *800.739.4137*. From jaylundgren at gmail.com Thu Apr 8 15:21:44 2021 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 8 Apr 2021 15:21:44 -0500 Subject: [Histonet] Is there a way to orient FFPE sections with an indicator that remains visible post staining? In-Reply-To: <1448590843.724181.1617903011238@mail.yahoo.com> References: <1448590843.724181.1617903011238.ref@mail.yahoo.com> <1448590843.724181.1617903011238@mail.yahoo.com> Message-ID: Those cassettes are for an auto-embedder and they are awful. Melt plain agar from micro in microwave, dye different colors with surgical ink, cool, cut cubes, embed with tissue. From yesyes at comcast.net Fri Apr 9 02:09:09 2021 From: yesyes at comcast.net (MARY ANN) Date: Fri, 09 Apr 2021 07:09:09 -0000 Subject: [Histonet] Ocular calibration Message-ID: <147532817.1.1617952144459@localhost> Can anyone share their ocular calibration procedure? Sent from Xfinity Connect Application From teri.johnson at decipherbio.com Fri Apr 9 11:09:07 2021 From: teri.johnson at decipherbio.com (Teri Johnson) Date: Fri, 9 Apr 2021 16:09:07 +0000 Subject: [Histonet] Temporary block and slide storage for referral specimens -nosig Message-ID: Good morning all and happy Friday! My company provides testing on FFPE material, and we received paraffin blocks and/or unstained slides and H&E stained slides. The blocks and H&E stained slides are returned to the originating path lab after the results are generated (currently 2-3 weeks after receipt). For those labs that do this type of work, how do you organize and store the material in a way that makes it easy and efficient to return? Are you using a particular system of containers, put things in numerical order, or by patient name, or other option? We receive about 60-100+ blocks a day and probably 20-40 H&E slides a day. Having to keep them for 2-3 weeks' time can give us a considerable inventory to manage. Thanks! Teri Johnson Sr. Histotechnician, Decipher Urologic Cancers From tbraud at holyredeemer.com Fri Apr 9 15:39:33 2021 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 9 Apr 2021 20:39:33 +0000 Subject: [Histonet] Microscope Calibration Message-ID: <48E053DDF6CE074DB6A7414BA05403F801E49C132E@HRHEX03-HOS.holyredeemer.local> A. USE EYEPIECE WITH OCULAR MICROMETER ALREADY IN PLACE. B. Place the stage micrometer on the stage and focus on some portion of the scale with the 10X objective. C. Looking through the microscope, examine the ruling of the stage micrometer so that you can distinguish between the large (0.1 mm.) and the small (0.01 mm.) divisions. D. Adjust the field so that the 0 line on the ocular micrometer is exactly superimposed upon the 0 line on the stage micrometer. E. Without moving the stage micrometer find another point at the extreme right where two other lines are exactly superimposed. The second set of superimposed lines should be as far as possible from the 0 lines, but the distance will vary with the objective and microscope. F. Knowing that each large division of the stage micrometer equals 0.1 mm. and each small division equals 0.01 mm., determine the total distance (in millimeters) between the two points of superimposition and the number of small ocular units necessary to cover the same distance (Figure 1). For example: Suppose 27 ocular units equal 2 large stage units or 0.2 mm. G. Calculate the number of millimeters that is measured by one small ocular unit. For example: Divide .2 mm. 27 = 0.0074 H. Since measurements of the protozoa and other small organisms or structures are usually given in microns rather than millimeters, the above determine must be converted to microns. Since 1 millimeter equals 1,000 microns, the millimeter determination multiplied by 1,000 will give the number of microns measured by 1 small ocular unit. For example: 0.0074 mm. X 1,000 = 7.4 microns (u). I. Record the calibrations of the ocular micrometer obtained with the 10X oculars combined with each of the three objectives. The lines of the stage micrometer will increase in magnification while those of the ocular micrometer will remain the same. With high and oil magnifications, the thinner ocular micrometer lined must be centered on the broader stage micrometer line for more accurate measurement. J. The size of any microscopic object can be quickly determined by measuring it with the ocular micrometer, noting the particular lens combination, and referring to the record of the ocular micrometer calibrations. RECORDING RESULTS: Record results of calibration on calibration card and is kept in the cabinet behind the scope in Special Microbiology. REFERENCES: Clinical Microbiology Procedures Handbook, Third edition, Vol 2. Lynne S. Garcia, ASM Press 2010. Date Approved by Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor HNL Labs, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From relia1 at earthlink.net Wed Apr 14 11:54:04 2021 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 14 Apr 2021 12:54:04 -0400 Subject: [Histonet] Leveraging your career opportunities as the pandemic ends -great info not just for histopeeps! Message-ID: <000001d7314e$c731f8b0$5595ea10$@earthlink.net> Hi Histopeeps, I wanted to give you a heads up!! My latest blog post is up on Fixation on Histology. I think it contains some great observations about the current job market and it could apply to everyone not just my histopeeps - I am hoping this is helpful to you and your friends and family. My latest article is entitled: "Leveraging Your Career Opportunities as the Pandemic ends." This is the link to my article on the NSH's Fixation on Histology Blog and it will also be published tomorrow in the next edition of the NSH Career Newsletter. Here is the link to the article: https://www.fixationonhistology.com/post/leveraging-your-career-opportunitie s-as-the-pandemic-ends If you have a minute to read it I would love to hear what you think. If you can't get to the article with this link let me know and I will send you a copy of it. Histopeeps, we also have amazing opportunities nationwide in! Texas California-Brand New Lab!!!/IHC/Generous relo/sign on Florida Generous relo and sign-on Virginia - 10-15K Sign on Bonus plus relo!! Oregon Maryland Kansas Pennsylvania South Carolina With new opportunities coming in from all over the U.S. daily. If you don't see your location PLEASE let me know. I might have the perfect position for you and might come in BEFORE the next newsletter!! Please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral reward! I LOVE TO GIVE REFERRAL REWARDS!! Some of these positions are RELIA exclusives! You won't see them anyplace else! My clients offer competitive pay rates, excellent benefits and in most cases Relocation assistance and/or a sign on bonus. They are ready to interview and hire!!! What more could you ask for? If You or Anyone You Know Might Be Interested In a New Opportunity, Please Contact Me ASAP If you want to chat ASAP call or text me on my cell At 407-353-5070. If you want some additional information or to set up a time to chat please call me toll free at 866-607-3542 or email me at relia1 at earthlink.net My clients are ready to interview and hire right away!!! And my phone is still ringing off the hook so if you don't see the location you want yet drop me a line so I can let you know when something DOES come up. I could be talking to a client about your next opportunity RIGHT NOW!! Have a great day. I look forward to hearing back from you. Thanks-Pam Right Time, Right Place, Right Move with RELIA! *16 Years!* Celebrating 16 years of service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From sandra.cheasty at wisc.edu Wed Apr 14 15:01:41 2021 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Wed, 14 Apr 2021 20:01:41 +0000 Subject: [Histonet] Cassette Labels Message-ID: Hello,r Has anyone has used printed labels successfully on cassettes that can withstand xylene/paraffin processing and long term storage? (I am NOT looking for a cassette printer...) Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From hdeziel at cnscro.com Thu Apr 15 07:38:19 2021 From: hdeziel at cnscro.com (Heather Deziel) Date: Thu, 15 Apr 2021 08:38:19 -0400 Subject: [Histonet] Tape Transfer Methods For Cryosectioned Brains In-Reply-To: <210c284cca9c0de705d880f731f4d917@cnscro.com> References: <210c284cca9c0de705d880f731f4d917@cnscro.com> Message-ID: <5769e79449f7ed6a864fc40aea4eb8bd@cnscro.com> Hello Histonet, I'm looking into working up a tape transfer method of collecting cryosections of brain while preserving infarct to be used in IHC. I find that when I try and section heavily damaged regions of the brain the tissue tears and and I lose it. Has anyone got any recommendations about the the Section-lab transfer tape (Kawamoto method), using the circuit plating tape recommended here (https://www.future-science.com/doi/full/10.2144/btn-2018-0021) or the cryojane system from Leica? Thank you, Heather Heather Deziel, MSc. Laboratory Technician, CNS|CRO 550 University Ave, Charlottetown, PE C1A 4P3 (a subsidiary of Neurodyn Life Sciences Inc.) From patpxs at gmail.com Thu Apr 15 09:29:35 2021 From: patpxs at gmail.com (Patpxs) Date: Thu, 15 Apr 2021 07:29:35 -0700 Subject: [Histonet] Tape Transfer Methods For Cryosectioned Brains In-Reply-To: <5769e79449f7ed6a864fc40aea4eb8bd@cnscro.com> References: <5769e79449f7ed6a864fc40aea4eb8bd@cnscro.com> Message-ID: Good Morning Heather, I have some questions about how you cut frozen brains. What temperature are you cutting at? How thick are your sections? How are your samples frozen? Flash freezing, slow freezing, iso-pentane in LN2? Your answers may provide clues to help you get better cryosections. Paula Sent from my iPhone > On Apr 15, 2021, at 5:39 AM, Heather Deziel via Histonet wrote: > > ?Hello Histonet, > > I'm looking into working up a tape transfer method of collecting > cryosections of brain while preserving infarct to be used in IHC. I > find that when I try and section heavily damaged regions of the brain > the tissue tears and and I lose it. Has anyone got any recommendations > about the the Section-lab transfer tape (Kawamoto method), using the > circuit plating tape recommended here > (https://www.future-science.com/doi/full/10.2144/btn-2018-0021) or the > cryojane system from Leica? > > Thank you, > > Heather > > Heather Deziel, MSc. > > Laboratory Technician, CNS|CRO 550 University Ave, Charlottetown, PE C1A > 4P3 > > (a subsidiary of Neurodyn Life Sciences Inc.) > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hdeziel at cnscro.com Thu Apr 15 13:24:01 2021 From: hdeziel at cnscro.com (Heather Deziel) Date: Thu, 15 Apr 2021 14:24:01 -0400 Subject: [Histonet] Tape Transfer Methods For Cryosectioned Brains In-Reply-To: References: <5769e79449f7ed6a864fc40aea4eb8bd@cnscro.com> Message-ID: <86f5bde9ca970bb24a3f5ddee47e7837@cnscro.com> Hi Paula, I am cutting at -24, but have tried going as warm as -18. I am currently learning with 30uM sections with the ultimate goal of moving towards 5 or 10uM. Our lab standard has been collecting into millonigs solution and doing free floating IHC. We generally have no issue with this technique, but do lose some of the peripheral damaged tissue near the infarct in our stroke brains. We're trying to work up painting the samples directly onto slides and skipping free floating staining to get a better end product. My current samples are very old, they were collected into 4%PFA in 2017 and cryoprotected by freezing in OCT cryomatrix in a little dish floating on LN2 in 2019. They've been stored at -80 since then. Usually we process the brains within a few weeks of collecting them so this particular tissue isn't our ideal situation. We're using old tissue to practice technique, so the current samples aren't going to be used for any actual analysis. When we heard about the tape method of collecting we were very curious as the the opinion other labs have about it. Have you tried it? Thanks for the answer! Heather Heather Deziel, MSc. Laboratory Technician, CNS|CRO 550 University Ave, Charlottetown, PE C1A 4P3 (a subsidiary of Neurodyn Life Sciences Inc.) On 2021-04-15 10:29, Patpxs wrote: > Good Morning Heather, > > I have some questions about how you cut frozen brains. > > What temperature are you cutting at? > How thick are your sections? > How are your samples frozen? Flash freezing, slow freezing, iso-pentane in LN2? > > Your answers may provide clues to help you get better cryosections. > > Paula > > Sent from my iPhone > >> On Apr 15, 2021, at 5:39 AM, Heather Deziel via Histonet wrote: >> >> Hello Histonet, >> >> I'm looking into working up a tape transfer method of collecting >> cryosections of brain while preserving infarct to be used in IHC. I >> find that when I try and section heavily damaged regions of the brain >> the tissue tears and and I lose it. Has anyone got any recommendations >> about the the Section-lab transfer tape (Kawamoto method), using the >> circuit plating tape recommended here >> (https://www.future-science.com/doi/full/10.2144/btn-2018-0021) or the >> cryojane system from Leica? >> >> Thank you, >> >> Heather >> >> Heather Deziel, MSc. >> >> Laboratory Technician, CNS|CRO 550 University Ave, Charlottetown, PE C1A >> 4P3 >> >> (a subsidiary of Neurodyn Life Sciences Inc.) >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward at wakehealth.edu Fri Apr 16 09:05:42 2021 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Fri, 16 Apr 2021 14:05:42 +0000 Subject: [Histonet] Filed slides sticking together Message-ID: I am posting this question for our Histology manager: Does anyone dry coverslipped slides in an oven before filing and if so how long and at what temperature. We are having issues with filed slides sticking together. Thanks in advance for your help with her question. Martha Ward, MT ASCP (QIHC) Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 From tpodawiltz at yahoo.com Fri Apr 16 10:57:26 2021 From: tpodawiltz at yahoo.com (Thomas Podawiltz) Date: Fri, 16 Apr 2021 15:57:26 +0000 (UTC) Subject: [Histonet] Filed slides sticking together In-Reply-To: References: Message-ID: <1557664378.3031344.1618588646612@mail.yahoo.com> I never have. Usually let them sit for two days before we filed them. Sent from Yahoo Mail for iPhone On Friday, April 16, 2021, 10:05 AM, Martha Ward-Pathology via Histonet wrote: I am posting this question for our Histology manager: Does anyone dry coverslipped slides in an oven before filing and if so how long and at what temperature.? We are having issues with filed slides sticking together. Thanks in advance for your help with her question. Martha? Ward, MT ASCP (QIHC) Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From terrie.dalton at icloud.com Fri Apr 16 12:57:23 2021 From: terrie.dalton at icloud.com (Theresa Dalton) Date: Fri, 16 Apr 2021 13:57:23 -0400 Subject: [Histonet] Filed slides sticking together In-Reply-To: <1557664378.3031344.1618588646612@mail.yahoo.com> References: <1557664378.3031344.1618588646612@mail.yahoo.com> Message-ID: <8BCA7015-64FC-4E75-BA70-89F43C105A0B@icloud.com> We leave ours in slide trays for at least 2 days prior to filing. We are a smaller derm lab so that?s probably not feasible for larger labs. Sent from my iPhone > On Apr 16, 2021, at 12:05 PM, Thomas Podawiltz via Histonet wrote: > > ?I never have. Usually let them sit for two days before we filed them. > > > Sent from Yahoo Mail for iPhone > > > On Friday, April 16, 2021, 10:05 AM, Martha Ward-Pathology via Histonet wrote: > > I am posting this question for our Histology manager: > > Does anyone dry coverslipped slides in an oven before filing and if so how long and at what temperature. We are having issues with filed slides sticking together. > > > Thanks in advance for your help with her question. > > Martha Ward, MT ASCP (QIHC) > Manager, Molecular Diagnostics Lab > Wake Forest Baptist Medical Center > Winston-Salem, NC 27157 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren at gmail.com Fri Apr 16 16:55:05 2021 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 16 Apr 2021 16:55:05 -0500 Subject: [Histonet] Filed slides sticking together In-Reply-To: <8BCA7015-64FC-4E75-BA70-89F43C105A0B@icloud.com> References: <1557664378.3031344.1618588646612@mail.yahoo.com> <8BCA7015-64FC-4E75-BA70-89F43C105A0B@icloud.com> Message-ID: I've seen labs that do this, like 30C for 24 hours, but if you use xylene, make sure to put the oven under a hood. It's a lot easier just to leave them out, in a well ventilated place, for 48hours. Buy more slide folders/trays. I worked one place where the slides were held for 24 hours before being given to the paths, because they were concerned about being exposed to xylene fumes. But that lab/hospital system had no competition within 100 miles and didn't care about turn around times. From john.garratt at ciqc.ca Fri Apr 16 19:25:18 2021 From: john.garratt at ciqc.ca (John Garratt) Date: Sat, 17 Apr 2021 00:25:18 +0000 Subject: [Histonet] Filed slides sticking together In-Reply-To: References: <1557664378.3031344.1618588646612@mail.yahoo.com> <8BCA7015-64FC-4E75-BA70-89F43C105A0B@icloud.com> Message-ID: Use tape if you want to have a lean mean operation. Instant filing, no sticking, no ovens. John On Fri, Apr 16, 2021 at 2:55 PM, Jay Lundgren via Histonet wrote: > I've seen labs that do this, like 30C for 24 hours, but if you use xylene, > make sure to put the oven under a hood. > > It's a lot easier just to leave them out, in a well ventilated place, for > 48hours. Buy more slide folders/trays. > > I worked one place where the slides were held for 24 hours before being > given to the paths, because they were concerned about being exposed to > xylene fumes. But that lab/hospital system had no competition within 100 > miles and didn't care about turn around times. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Fri Apr 16 22:11:54 2021 From: patpxs at gmail.com (Patpxs) Date: Fri, 16 Apr 2021 20:11:54 -0700 Subject: [Histonet] Filed slides sticking together In-Reply-To: References: Message-ID: <9D54D8A9-A88F-4F6E-AB42-1DB7AA3B5C2C@gmail.com> We used to use a drying oven set at 37 degrees C. We stopped using it because some people thought it smelled, plus our slide volume increased to the point where all the flats couldn?t fit. Now we dry them for two days, minimum, before filing. I prefer to let them dry for at least 4 days. Paula Sent from my iPhone > On Apr 16, 2021, at 5:26 PM, John Garratt via Histonet wrote: > > ?Use tape if you want to have a lean mean operation. Instant filing, no sticking, no ovens. > > John > >> On Fri, Apr 16, 2021 at 2:55 PM, Jay Lundgren via Histonet wrote: >> >> I've seen labs that do this, like 30C for 24 hours, but if you use xylene, >> make sure to put the oven under a hood. >> >> It's a lot easier just to leave them out, in a well ventilated place, for >> 48hours. Buy more slide folders/trays. >> >> I worked one place where the slides were held for 24 hours before being >> given to the paths, because they were concerned about being exposed to >> xylene fumes. But that lab/hospital system had no competition within 100 >> miles and didn't care about turn around times. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Fri Apr 16 22:27:35 2021 From: patpxs at gmail.com (Patpxs) Date: Fri, 16 Apr 2021 20:27:35 -0700 Subject: [Histonet] Tape Transfer Methods For Cryosectioned Brains In-Reply-To: <86f5bde9ca970bb24a3f5ddee47e7837@cnscro.com> References: <86f5bde9ca970bb24a3f5ddee47e7837@cnscro.com> Message-ID: <176ADC29-EAC5-4E21-BB12-E17209DEC4C1@gmail.com> Hi Heather, I can see why you?re having trouble. 30 micron sections are inherently unstable. Like paraffin, the thicker a section is the more difficult it is to cut. Plus since your practice samples are old they tend to be more brittle. Try cutting at 5 microns and see what happens. Remember to let the samples warm up in the cryostat if they have been stored at -70 or -80 degrees. If they are too cold they are brittle too. I have not tried the tape method. We just place the sections directly on the slide. Paula Sent from my iPhone > On Apr 15, 2021, at 11:24 AM, Heather Deziel wrote: > > ? > Hi Paula, > > I am cutting at -24, but have tried going as warm as -18. I am currently learning with 30uM sections with the ultimate goal of moving towards 5 or 10uM. Our lab standard has been collecting into millonigs solution and doing free floating IHC. We generally have no issue with this technique, but do lose some of the peripheral damaged tissue near the infarct in our stroke brains. We're trying to work up painting the samples directly onto slides and skipping free floating staining to get a better end product. > > My current samples are very old, they were collected into 4%PFA in 2017 and cryoprotected by freezing in OCT cryomatrix in a little dish floating on LN2 in 2019. They've been stored at -80 since then. Usually we process the brains within a few weeks of collecting them so this particular tissue isn't our ideal situation. We're using old tissue to practice technique, so the current samples aren't going to be used for any actual analysis. When we heard about the tape method of collecting we were very curious as the the opinion other labs have about it. Have you tried it? > > Thanks for the answer! > > Heather > > Heather Deziel, MSc. > Laboratory Technician, CNS|CRO > 550 University Ave, Charlottetown, PE C1A 4P3 > > (a subsidiary of Neurodyn Life Sciences Inc.) > > > >> On 2021-04-15 10:29, Patpxs wrote: >> >> Good Morning Heather, >> >> I have some questions about how you cut frozen brains. >> >> What temperature are you cutting at? >> How thick are your sections? >> How are your samples frozen? Flash freezing, slow freezing, iso-pentane in LN2? >> >> Your answers may provide clues to help you get better cryosections. >> >> Paula >> >> Sent from my iPhone >> >>> On Apr 15, 2021, at 5:39 AM, Heather Deziel via Histonet wrote: >>> >>> Hello Histonet, >>> >>> I'm looking into working up a tape transfer method of collecting >>> cryosections of brain while preserving infarct to be used in IHC. I >>> find that when I try and section heavily damaged regions of the brain >>> the tissue tears and and I lose it. Has anyone got any recommendations >>> about the the Section-lab transfer tape (Kawamoto method), using the >>> circuit plating tape recommended here >>> (https://www.future-science.com/doi/full/10.2144/btn-2018-0021) or the >>> cryojane system from Leica? >>> >>> Thank you, >>> >>> Heather >>> >>> Heather Deziel, MSc. >>> >>> Laboratory Technician, CNS|CRO 550 University Ave, Charlottetown, PE C1A >>> 4P3 >>> >>> (a subsidiary of Neurodyn Life Sciences Inc.) >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Thu Apr 22 11:21:22 2021 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 22 Apr 2021 12:21:22 -0400 Subject: [Histonet] Happy Lab Week Avengers of the Lab!! Check out the ASCP logo for Lab Week 2021! Message-ID: <088001d73793$88d6d5e0$9a8481a0$@earthlink.net> Hello Histopeeps!! I hope you are doing well. I wanted to send a special bulletin wishing you a Happy National Medical Laboratory Professionals Week. I Hope Your Lab Has Been Having Some Fun, Informative & Special Events To Celebrate Because You Deserve It! The theme for lab week this year is ?Avengers of the Lab? Let me know and I will be happy to send you the logo. I have heard of some really cool things that labs are doing to mark the occasion including luncheons, costume, trivia and photo contests and displays to educate others about what you do. If you get a chance check out the ASCP?s website section for Lab week. Here is the link: ?http://www.ascp.org/labweek I would love to hear what you are doing in your lab to celebrate so please shoot me back an e-mail and let me know. Histopeeps, I also wanted to let you know about some exciting new positions I am working on. HOT HISTO JOBS!!! 10-15K Sign On bonuses! Florida, California, Virginia! I also have exciting opportunities in: ? Austin, TX ? Bend, OR ? Greenville, SC ? 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(407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From Cheryl.Newman-Bodden at va.gov Fri Apr 23 09:30:15 2021 From: Cheryl.Newman-Bodden at va.gov (Newman-Bodden, Cheryl L.) Date: Fri, 23 Apr 2021 14:30:15 +0000 Subject: [Histonet] Job Opportunities USA Jobs Message-ID: I just wanted to give a notice to anyone looking for a histology job in the Tampa Bay area, with a four year degree, there will be several jobs available in the near future at the Tampa VA and will be listed on the USA Jobs website. Unfortunately, these postings have very short windows and when they close, they do not open again for 90 days. If I know of an applicant interested in a position, I can send an email when the window opens for accepting applications. Even if someone is not interested in a job presently, I would still encourage them to inquire; some openings will not be available until months from now. Cheryl Bodden, BS, HTL(ASCP) Histology Supervisor Supervisor Health Science Specialist Pathology and Laboratory Medicine James A. Haley Veterans Hospital 13000 Bruce B. Downs Blvd. Tampa, FL 33612 (813) 972-2000 ext. 7844 From jaylundgren at gmail.com Fri Apr 23 11:00:30 2021 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 23 Apr 2021 11:00:30 -0500 Subject: [Histonet] Job Opportunities USA Jobs In-Reply-To: References: Message-ID: Just curious, do you have to have a Florida license to work for the VA in Florida? Do you know the GS rating of the job? From Cheryl.Newman-Bodden at va.gov Fri Apr 23 11:54:10 2021 From: Cheryl.Newman-Bodden at va.gov (Newman-Bodden, Cheryl L.) Date: Fri, 23 Apr 2021 16:54:10 +0000 Subject: [Histonet] [EXTERNAL] Re: Job Opportunities USA Jobs In-Reply-To: References: Message-ID: GS 9 and no, you do not need a FL license for the VA. You do however, have to have an ASCP (American Society for Clinical Pathology) HTL (Histotechnologist) license. From: Jay Lundgren Sent: Friday, April 23, 2021 12:01 PM To: Newman-Bodden, Cheryl L. Cc: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Job Opportunities USA Jobs Just curious, do you have to have a Florida license to work for the VA in Florida? Do you know the GS rating of the job? From shultz11 at cox.net Fri Apr 23 12:22:35 2021 From: shultz11 at cox.net (kendra shultz) Date: Fri, 23 Apr 2021 12:22:35 -0500 Subject: [Histonet] (no subject) Message-ID: <89517dcb-4f94-469c-a2b8-8c11d94b0a96@email.android.com> Any suggestions on processing necropsy muscle. Sections are dry and pieces are falling off during H&E. We process many other tissue types but only have problems with muscle. Thanks, Kendra Shultz From relia1 at earthlink.net Tue Apr 27 11:03:59 2021 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 27 Apr 2021 12:03:59 -0400 Subject: [Histonet] Best Interview Question Ever!! And it's from Facebook!! Plus 10-15 in relo/sign on bonuses with MY clients! Message-ID: <000001d73b7e$ef7e0310$ce7a0930$@earthlink.net> Dear Histopeeps, I hope you are having a great week! Coming soon to an interview near you Have you seen Facebook?s Million Dollar interview question? Here it is ?On your very best day at work The day you come home and think you have the best job in the world What did you do that day?? Facebook uses this question to find out what their candidates are passionate about. Histopeeps, If you were asked this question how would you answer it? If your answer doesn?t match up with a day at your current position we need to talk! I can help you find the position that you are passionate about, the place where every day is your best day at work. There are many techs that I have worked with over the years that can answer that question with the enthusiasm it deserves because I helped them find that job. Let me help you too. If you are looking for a job today, tomorrow, in 3 months, 6 months, a year, 5 years. I am here for you. My best day at work is every day since my passion is to find the right opportunity for you! All of my opportunities are full time permanent positions with some of the leading employers nationwide. There are 1st, 2nd and 3rd shift positions open. Experienced and entry level, supervisory and tech, on and off the bench positions and ASCP or eligible opportunities!! My clients offer excellent compensation, benefits and up to 15K in relocation/sign on bonuses. And they can?t wait to speak to you!!! 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Please contact me at relia1 at earthlink.net or toll free at 866-607-3542 or call/text me on my cell at 407-353-5070 if you are interested in more information on any of these positions or if you would like for me to work on a custom job search JUST for you. Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From shultz11 at cox.net Thu Apr 29 08:21:14 2021 From: shultz11 at cox.net (kendra shultz) Date: Thu, 29 Apr 2021 08:21:14 -0500 Subject: [Histonet] Job Message-ID: <1249b02d-292e-4655-8268-ccadf43d8e4f@email.android.com> Louisiana State University Histology/IHC Research Associate 3 position Apply online at lsu.edu Thanks, Kendra Shultz From ASelf at tidelandshealth.org Thu Apr 29 12:43:28 2021 From: ASelf at tidelandshealth.org (Amy Self) Date: Thu, 29 Apr 2021 17:43:28 +0000 Subject: [Histonet] Molecular Ordering in Pathology Message-ID: How is your lab handling molecular orders that are coming from outside physician offices? We currently receive molecular orders via fax, and this is getting quite cumbersome. When we were only getting a few a week it was tolerable but now we are getting a few orders daily. What should be a simple process takes entirely too long because we end up having to call the physician's office to clarify the order or ask which PDL-1 you want, ICD-10 codes, etc. and then go to the reference lab website and order the test. I just feel there is some simpler way of doing this. I would like to see the ordering physician's office order their own test from the reference lab - we get a request to send the block out and it's over. Am I Hopeless in histology for wanting or expecting a simpler workflow for molecular orders? Our IT department is trying to simplify things but no matter what they come up with it seems to create more work for us in histology. We have Meditech LIS system. Just to throw this out there too - we have no pathology secretary or a mail-out department; we histotechs do all this work within the histology department ourselves. Thanks, Amy Self Senior Histology Technologist Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 Office: (843) 520-8711 aself at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From 11z at comcast.net Fri Apr 30 12:15:00 2021 From: 11z at comcast.net (11z at comcast.net) Date: Fri, 30 Apr 2021 10:15:00 -0700 Subject: [Histonet] cassette labeler Message-ID: <000f01d73de4$5f001e00$1d005a00$@comcast.net> HI. I am using a TBS cassette printer and it is on the blink. What are others using these days for cassette printers? Does anyone have an older one they wish to part with? The new ones are very expensive, out of my budget LOL Thanks LeRoy Brown HT(ASCP) HTL HCS, Inc.