From nelsonrnch at verizon.net Thu Oct 1 09:33:16 2020 From: nelsonrnch at verizon.net (Patti Nelson - PN Lab Consultant) Date: Thu, 1 Oct 2020 14:33:16 +0000 (UTC) Subject: [Histonet] PART TIME / PER DIEM HISTOTECH IN MURRIETA, CALIF References: <1538358175.719826.1601562796816.ref@mail.yahoo.com> Message-ID: <1538358175.719826.1601562796816@mail.yahoo.com> Hello Histonet, WANTED: Experience preferred histotech/lab assistant for a high volume, fast pace GI laboratory in Murrieta, California.Interested parties email resumes to Symantha.Murphy at unitedmd.com. Sincerely, PATTI NELSON? H.T.(ASCP) 909-841-9761nelsonrnch at verizon.netCONFIDENTIALITY NOTICE:This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants?and are intended only for the addressee.?The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law.? Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful.? If you are not the addressee, please promptly delete this message and notify the sender ofthe delivery error by e-mail or you may call? 909-841-9761. From ariel at cta-lab.com Thu Oct 1 10:39:40 2020 From: ariel at cta-lab.com (Ariel Liberda) Date: Thu, 01 Oct 2020 15:39:40 +0000 Subject: [Histonet] Cassette and slide printer LIS systems Message-ID: I hope everyone is having a good day! My lab is looking to implement cassette printers and slide printing LIS systems. We currently hand write on all of our cassettes and slides. I was wondering which systems people like/dont like? What are the pros and cons? Thank you all so much in advance! I feel like I've been relying on the group of expertise a lot lately! -Ari -- Ariel Liberda Lead Histotech CTALab c. [503.906.7300](https://urldefense.proofpoint.com/v2/url?u=https-3A__webmail.networksolutionsemail.com_edgedesk_cgi-2Dbin_tel-3A503.906.7300&d=DQMFAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=HKyZElUZ9A6Yyf6DYydB4h3JuSpSmPINBXYcEI2kzIM&m=kk9btPrCg75YkgEZBePfoDnCBgWXID9HnQfCIK51c80&s=Sd5gQGqV6e2f-nZgaLPMCVPVBCSZEDZQCN-XstC2-74&e=) | f. [503.245.8219](https://urldefense.proofpoint.com/v2/url?u=https-3A__webmail.networksolutionsemail.com_edgedesk_cgi-2Dbin_tel-3A503.245.8219&d=DQMFAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=HKyZElUZ9A6Yyf6DYydB4h3JuSpSmPINBXYcEI2kzIM&m=kk9btPrCg75YkgEZBePfoDnCBgWXID9HnQfCIK51c80&s=Ba4mH8xxABXuh3rvnefIcx6NSVFWm_tOxUI1yrS533k&e=) 12254 SW Garden Pl. | Tigard, Oregon 97223 Your skin, hair and nail pathology experts! From narasimj at ohsu.edu Fri Oct 2 09:53:16 2020 From: narasimj at ohsu.edu (Jayasri Narasimhan) Date: Fri, 2 Oct 2020 14:53:16 +0000 Subject: [Histonet] Experience Documentation form vs. Work experience form (HTL Route 2)? Message-ID: <5da04327969142e08a03d7556dcdb8d9@ohsu.edu> Hi everyone, I got an update (Deficient) regarding my HTL application that I submitted mid-August. However, I'm unable to find the form that they are requesting. I got the one I used by downloading the form under "Applicant Resources" under the HTL category. This is the response: We received yoir work experience form and letter of authenticity completed by _________ however it is not the most recent form. Email revised forms to DocumentationSC.Boc at Ascp.org We require an experience documentation form(s). The experience documentation form must be completed by your immediate supervisor or laboratory director and attached to a letter of authenticity signed by this individual verifying the authenticity of the form(s). This letter must be printed on original letterhead and state that the experience documentation form(s) was completed by the employer, with the date and signature. Download and print the form from the ASCP website at www.ascp.org. Is there another form out there? Would anyone be able to email the latest one to me? Thank you, JN From sara.a.wells210 at gmail.com Fri Oct 2 10:13:14 2020 From: sara.a.wells210 at gmail.com (Sara Wells) Date: Fri, 2 Oct 2020 09:13:14 -0600 Subject: [Histonet] Experience Documentation form vs. Work experience form (HTL Route 2)? In-Reply-To: <5da04327969142e08a03d7556dcdb8d9@ohsu.edu> References: <5da04327969142e08a03d7556dcdb8d9@ohsu.edu> Message-ID: Hi Jayasri, It's a little different from when I did my HTL application due to COVID-19, but I used this form: https://www.ascp.org/content/docs/default-source/boc-pdfs/boc-us-routes/htl_route_2_3_doc.pdf?sfvrsn=2 I had to print it out, sign the information that I could on the page for myself, send this to my immediate supervisor, have him send it back to me, and then I could send it off to the BOC people. I also had to make sure the letter of authenticity was in there on the original letterhead. After that, everything was fine. I think the documentation has to be hand written, and the letter could be typed as long as it states something like " I ______ on this official letterhead, agree that _______ has the knowledge and work experience on the work documentation form to take the HTL exam through route 2." Something along those lines and have them sign it. I hope this helps. It's a little different since it seems like you have to send electronically now. If you have more issues, I would try chatting with them online or calling. Sara On Fri, Oct 2, 2020 at 9:00 AM Jayasri Narasimhan via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi everyone, > > > I got an update (Deficient) regarding my HTL application that I submitted > mid-August. However, I'm unable to find the form that they are requesting. > I got the one I used by downloading the form under "Applicant Resources" > under the HTL category. > > > This is the response: > > > We received yoir work experience form and letter of authenticity completed > by _________ however it is not the most recent form. Email revised forms to > DocumentationSC.Boc at Ascp.org > > We require an experience documentation form(s). The experience > documentation form must be completed by your immediate supervisor or > laboratory director and attached to a letter of authenticity signed by this > individual verifying the authenticity of the form(s). This letter must be > printed on original letterhead and state that the experience documentation > form(s) was completed by the employer, with the date and signature. > Download and print the form from the ASCP website at www.ascp.org< > http://www.ascp.org/>. > > > > > Is there another form out there? Would anyone be able to email the latest > one to me? > > > Thank you, > > JN > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *Sara Wells* From narasimj at ohsu.edu Fri Oct 2 10:18:18 2020 From: narasimj at ohsu.edu (Jayasri Narasimhan) Date: Fri, 2 Oct 2020 15:18:18 +0000 Subject: [Histonet] Experience Documentation form vs. Work experience form (HTL Route 2)? In-Reply-To: References: <5da04327969142e08a03d7556dcdb8d9@ohsu.edu>, Message-ID: <66557cadff584498b9515c0db6364ecb@ohsu.edu> Thank you, Sara! That's the form I sent along with a letterhead letter of authenticity. I called ASCP just now - It is possible that I didn't include that second page and that is the problem. The second page just has guidelines so I think my supervisor didn't scan it. Hopefully, that will fix it! Best, Jay ________________________________ From: Sara Wells Sent: Friday, October 2, 2020 8:13:14 AM To: Jayasri Narasimhan Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Experience Documentation form vs. Work experience form (HTL Route 2)? Hi Jayasri, It's a little different from when I did my HTL application due to COVID-19, but I used this form: https://www.ascp.org/content/docs/default-source/boc-pdfs/boc-us-routes/htl_route_2_3_doc.pdf?sfvrsn=2 I had to print it out, sign the information that I could on the page for myself, send this to my immediate supervisor, have him send it back to me, and then I could send it off to the BOC people. I also had to make sure the letter of authenticity was in there on the original letterhead. After that, everything was fine. I think the documentation has to be hand written, and the letter could be typed as long as it states something like " I ______ on this official letterhead, agree that _______ has the knowledge and work experience on the work documentation form to take the HTL exam through route 2." Something along those lines and have them sign it. I hope this helps. It's a little different since it seems like you have to send electronically now. If you have more issues, I would try chatting with them online or calling. Sara On Fri, Oct 2, 2020 at 9:00 AM Jayasri Narasimhan via Histonet > wrote: Hi everyone, I got an update (Deficient) regarding my HTL application that I submitted mid-August. However, I'm unable to find the form that they are requesting. I got the one I used by downloading the form under "Applicant Resources" under the HTL category. This is the response: We received yoir work experience form and letter of authenticity completed by _________ however it is not the most recent form. Email revised forms to DocumentationSC.Boc at Ascp.org We require an experience documentation form(s). The experience documentation form must be completed by your immediate supervisor or laboratory director and attached to a letter of authenticity signed by this individual verifying the authenticity of the form(s). This letter must be printed on original letterhead and state that the experience documentation form(s) was completed by the employer, with the date and signature. Download and print the form from the ASCP website at www.ascp.org. Is there another form out there? Would anyone be able to email the latest one to me? Thank you, JN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Sara Wells From rgonzalez at origene.com Mon Oct 5 10:22:31 2020 From: rgonzalez at origene.com (Rachel Gonzalez) Date: Mon, 5 Oct 2020 15:22:31 +0000 Subject: [Histonet] Shandon Micro Writer software Message-ID: Hi I am trying to reactivate a lab dinosaur. It was bought and used for a year then shelved. The computer is long gone but I would like to use it. Lamb; Shandon Micro Writer for cassettes Model No: E22.01MWR Does anyone have the software? Thanks for your help. M2 Disclaimer The information contained in this communication from the sender is confidential. It is intended solely for use by the recipient and others authorized to receive it. If you are not the recipient, you are hereby notified that any disclosure, copying, distribution or taking action in relation of the contents of this information is strictly prohibited and may be unlawful. This email has been scanned for viruses and malware, and may have been automatically archived by Mimecast Ltd, an innovator in Software as a Service (SaaS) for business. Providing a safer and more useful place for your human generated data. Specializing in; Security, archiving and compliance. To find out more visit the Mimecast website. From relia1 at earthlink.net Tue Oct 6 11:53:46 2020 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 6 Oct 2020 12:53:46 -0400 Subject: [Histonet] RELIA Careers Bulletin 10-6-2020 I will be speaking at the NSH Virtual Convention and I have some exciting NEW opportunities - Check it out! :) Message-ID: <000001d69c01$41eba170$c5c2e450$@earthlink.net> Hi Histopeeps, How are you? As you know I do the majority of my work via email and phone so I rarely get a chance to meet people in person. That is one of the many reasons I enjoy attending the NSH conventions. Like most other meetings and gatherings in 2020 the NSH will be held virtually. I wanted to drop you a line and see if you or anyone you work with is planning on attending the NSH convention ON LINE next week? If so I would love to have the opportunity to say Hi. I will be very easy to find because I will be speaking LIVE at the NSH membership booth on Tuesday 10/13, Wednesday 10/14 and Thursday 10/15 from 1:00 to 1:15 eastern. My topics will be: Day One - Career Planning Day Two - Education&Qualifications; What do employers look for? Day Three - Advocacy There is still time to register for the convention - what an amazing way to experience the NSH Symposium Convention from the comfort of your home! Here is a link for more info: https://l.feathr.co/landing-page---46th-annual-virtual-symposium/convention- -pam-barker I have some exciting leadership and technical opportunities! . North Carolina Management and Tech positions. . North Carolina IHC Specialist . SW Florida Dermpath Histotech . California Multiple Shifts Learn new things!! . Virginia Pathologists' Assistant. . Tennessee Flow Cytometrist . New Mexico Histology Tech . New York Great oppty for entry level or senior techs . Kentucky Senior Tech All of these positions are full time and permanent and most of my clients offer relocation assistance and or a sign on bonus. For more info please contact me at relia1 at earthlink.net or on my cell/text at 407-353-5070 or toll free at the office at 866-607-3542. Hope to see you online! J Have a great day!! Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From ASelf at tidelandshealth.org Tue Oct 6 13:51:17 2020 From: ASelf at tidelandshealth.org (Amy Self) Date: Tue, 6 Oct 2020 18:51:17 +0000 Subject: [Histonet] Temperature Checks Message-ID: Good Afternoon, Do any of you get your temperatures for your equipment and\or refrigerators monitored by a temperature monitoring company and if so how do you document this check? Thanks in Advance, Amy Self Senior Histology Technologist Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 Office: (843) 520-8711 aself at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From ricki.simoskevitz at medtronic.com Wed Oct 7 12:04:49 2020 From: ricki.simoskevitz at medtronic.com (Simoskevitz, Ricki) Date: Wed, 7 Oct 2020 17:04:49 +0000 Subject: [Histonet] Temperature Checks In-Reply-To: References: Message-ID: Our facilities department is in charge of this. If there is a problem they get an alarm from the company. It takes temperature readings every hour on some equipment. On other equipment it is more often. You can log in any time and see your temperatures for any day, time etc, Ricki Simoskevitz Scientist Group Leader OI Lab | Eatontown Quality Medtronic Osteoinductivity Lab 51 James Way| Eatontown, NJ/07724 | USA Office 732-578-6604|Fax 763-355-1224 ricki.simoskevitz at medtronic.com Medtronic.com|Facebook|Linkedin|Twitter|YouTube LET'S TAKE HEALTHCARE FURTHER, TOGETHER -----Original Message----- From: Amy Self Sent: Tuesday, October 6, 2020 2:51 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Temperature Checks Good Afternoon, Do any of you get your temperatures for your equipment and\or refrigerators monitored by a temperature monitoring company and if so how do you document this check? Thanks in Advance, Amy Self Senior Histology Technologist Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 Office: (843) 520-8711 aself at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From Timothy.Morken at ucsf.edu Wed Oct 7 13:00:55 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 7 Oct 2020 18:00:55 +0000 Subject: [Histonet] FW: Temperature Checks In-Reply-To: References: Message-ID: Amy, we have been using Awarepoint, but they are apparently going out of business so we are switching over to Stanley Aeroscout on all fridges, freezers and room temp/humidity throughout our facilities. We have direct access to the monitoring of each "asset" and can check it online at any time from any computer or mobile. We get email and text (and/or pager) alerts when it goes out of range for 30 minutes, the door is open (via door sensor) or immediately if the power goes off. We have multiple people set up to receive alerts in a hierarchy of escalations. We can see/print a report at any time of the per-minute temperature history of any asset. We can also print a report showing the temperature of each asset at a given time of day (like the old manual checks). But with immediate access to real-time temperature and graphs that is not useful anymore. We used to do a daily "check" of the online recording "to make sure the system is working" but after many years of zero problems we stopped doing that. Our Facilities Department maintains the system and sends out a notice if the network system goes down. The ONLY time we do manual checks is if the system goes down because we would not get alerts in that case. That has not happened in over two years. So we do not check it daily. We only check if we get an alert or if we suspect a problem. The "Tag" that is on each asset has a digital display of temperature at all times. If it goes out of range there is an audible alarm (if anyone is around to hear it) as well as the alert sent via email and text message. It records the temperature of every asset once per minute. The tag keeps recording even if the network goes down and once the system comes back up it will upload all the data to the central system once contact is restored. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center LET'S TAKE HEALTHCARE FURTHER, TOGETHER -----Original Message----- From: Amy Self > Sent: Tuesday, October 6, 2020 2:51 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Temperature Checks Good Afternoon, Do any of you get your temperatures for your equipment and\or refrigerators monitored by a temperature monitoring company and if so how do you document this check? Thanks in Advance, Amy Self Senior Histology Technologist Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 Office: (843) 520-8711 aself at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: https://urldefense.proofpoint.com/v2/url?u=http-3A__emaildisclaimer.medtronic.com&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=iY3yXm3PHBrYiJmNEaRhTf88wbgJlmeu3OJV0BhVebI&s=R_MvMwmBylgGoT7MxcG2NOlqTf0IFz_tiodHwe1U7h4&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=iY3yXm3PHBrYiJmNEaRhTf88wbgJlmeu3OJV0BhVebI&s=OZAoRr_3DRcm4o2f0k6EQpLGlbxIND0fhovgbGfQVbI&e= From m.jamison at elitechgroup.com Wed Oct 7 13:55:27 2020 From: m.jamison at elitechgroup.com (Michelle Jamison) Date: Wed, 7 Oct 2020 12:55:27 -0600 Subject: [Histonet] Mycobacteria prep, MycoPrep question Message-ID: <8b738473-9fd8-e651-558a-362f41db960f@elitechgroup.com> Hi All We are trying to simulate a matrix as a stand in for a patient sample. Currently using a Porcine stomach recipe. When we use MycoPrep (NALC-NaOH) the result is small chunks that drop to the bottom of a column and liquid staying on top. My question: Is this what happens to a real sample? Thanks much for any input, especially if you have a recipe you would like to share for artificial matrix! Michelle -- Best of all things to you, Michelle Jamison // Tel : +1.435.752.6011 Ext. 435.227.1474 m.jamison at elitechgroup.com ? www.elitechgroup.com ------------------------------------------------------------------------ Logo From igor.deyneko at gmail.com Wed Oct 7 14:11:10 2020 From: igor.deyneko at gmail.com (Igor Deyneko) Date: Wed, 7 Oct 2020 15:11:10 -0400 Subject: [Histonet] Temperature Checks In-Reply-To: References: Message-ID: Hello, We are actually in transition from manual temperature reading to an automated system called CenTrak, managed by Infinite Leap. We are doing a test run with them for our Clinical lab(Hematology, Microbiology, Chemistry and Blood Bank). They installed sensors on freezers, fridges, water baths, incubators and ambient room sensors too. We establish the parameters, go through the validation process. Then we set up alarms/emails when certain equipment is out of range. Everything is centralized in a web page from which we can monitor all sensors. Igor Deyneko Pathology Manager Montefiore Medical Center Bronx, NY On Wed, Oct 7, 2020 at 1:27 PM Simoskevitz, Ricki via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Our facilities department is in charge of this. If there is a problem > they get an alarm from the company. It takes temperature readings every > hour on some equipment. On other equipment it is more often. You can log > in any time and see your temperatures for any day, time etc, > > Ricki Simoskevitz > Scientist Group Leader OI Lab | Eatontown Quality > > Medtronic > Osteoinductivity Lab > 51 James Way| Eatontown, NJ/07724 | USA > Office 732-578-6604|Fax 763-355-1224 > ricki.simoskevitz at medtronic.com > Medtronic.com|Facebook|Linkedin|Twitter|YouTube > > > LET'S TAKE HEALTHCARE > FURTHER, TOGETHER > > -----Original Message----- > From: Amy Self > Sent: Tuesday, October 6, 2020 2:51 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Temperature Checks > > Good Afternoon, > > Do any of you get your temperatures for your equipment and\or > refrigerators monitored by a temperature monitoring company and if so how > do you document this check? > > Thanks in Advance, > Amy Self > Senior Histology Technologist > Tidelands Georgetown Memorial Hospital > 606 Black River Road > Georgetown, SC 29440 > Office: (843) 520-8711 > aself at tidelandshealth.org > Our mission: We help people live better lives through better health. > > > NOTE: > The information contained in this message may be privileged, confidential > and protected from disclosure. If the reader of this message is not the > intended recipient, or an employee or agent responsible for delivering this > message to the intended recipient, you are hereby notified that any > dissemination, distribution or copying of this communication is strictly > prohibited. If you have received this communication in error, please notify > us immediately by replying to this message and deleting it from your > computer. > Thank you. > [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email > is proprietary to Medtronic and is intended for use only by the individual > or entity to which it is addressed, and may contain information that is > private, privileged, confidential or exempt from disclosure under > applicable law. If you are not the intended recipient or it appears that > this mail has been forwarded to you without proper authority, you are > notified that any use or dissemination of this information in any manner is > strictly prohibited. In such cases, please delete this mail from your > records. To view this notice in other languages you can either select the > following link or manually copy and paste the link into the address bar of > a web browser: http://emaildisclaimer.medtronic.com > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From 1056149864 at qq.com Thu Oct 8 03:20:52 2020 From: 1056149864 at qq.com (=?gb18030?B?zs/Fow==?=) Date: Thu, 8 Oct 2020 16:20:52 +0800 Subject: [Histonet] Golgi staining problems Message-ID: Can Golgi staining be performed with preperfused brain tissue? And after the completion of perfusion, the brain tissue has been cryopreserved for more than a year, can it still be used? What to do if available From acanabal at ciencias.unam.mx Thu Oct 8 13:02:43 2020 From: acanabal at ciencias.unam.mx (=?UTF-8?Q?Alonso_Mart=C3=ADnez_Canabal?=) Date: Thu, 8 Oct 2020 13:02:43 -0500 Subject: [Histonet] Golgi staining problems In-Reply-To: References: Message-ID: Good morning, Golgi-Cox method does not suit well with prefixed tissue. With short fixation time in formaldehyde you have poorly stained neurons and many astrocytes, with a prolonged fixation time you only see astrocytes. The silver nitrate method uses formaldehyde but I am not sure if it is compatible with prefixation in formaldehyde. El jue., 8 oct. 2020 a las 3:27, ?? via Histonet (< histonet at lists.utsouthwestern.edu>) escribi?: > Can Golgi staining be performed with preperfused brain tissue? And after > the completion of perfusion, the brain tissue has been cryopreserved for > more than a year, can it still be used? What to do if available > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Dr. Alonso Mart?nez Canabal PhD Profesor Asociado "C" Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833 From bwood at IUHealth.org Thu Oct 8 13:25:12 2020 From: bwood at IUHealth.org (Wood, Barry A) Date: Thu, 8 Oct 2020 18:25:12 +0000 Subject: [Histonet] CAP required PT Message-ID: For which IHC stains is it required by CAP to do proficiency testing? Barry Wood CT (ASCP) Director of Anatomic Pathology Indiana University Health 317-491-6351 bwood at iuhealth.org Compassionate. Highly skilled. Personalized. Visit iuhealth.org From criley at dpspa.com Fri Oct 9 09:14:00 2020 From: criley at dpspa.com (Charles Riley) Date: Fri, 9 Oct 2020 14:14:00 +0000 Subject: [Histonet] Alcian Blue staining Message-ID: We are having an issue where the goblet cells in our control tissue are not staining pink even though the patient tissue is staining beautifully on the same slide. The stain is done manually. What can be some causes for the issues in staining on the control section? From Daniel.Pesino at TrinityHealthOfNE.org Fri Oct 9 09:24:22 2020 From: Daniel.Pesino at TrinityHealthOfNE.org (Daniel Pesino) Date: Fri, 9 Oct 2020 14:24:22 +0000 Subject: [Histonet] Alcian Blue staining In-Reply-To: References: Message-ID: Hi Charles - your control tissue may be autolyzed. Try using a well-processed control tissue. If that's not it, make sure that your control tissue is being completely deparaffinized and that it is being totally covered with the stains. Good luck! Daniel Pesino, HTL(ASCP)QIHC CM Senior Histotechnologist Trinity Health Of New England Daniel.Pesino at TrinityHealthOfNE.org W? 860-714-4675 114 Woodland Street Hartford, CT 06105 TrinityHealthOfNE.org | Facebook | Twitter | Instagram -----Original Message----- From: Charles Riley via Histonet Sent: Friday, October 9, 2020 10:14 AM To: histonet at lists.utsouthwestern.edu Subject: [External] [Histonet] Alcian Blue staining Warning: This email originated from the Internet! DO NOT CLICK links if the sender is unknown, and NEVER provide your password. We are having an issue where the goblet cells in our control tissue are not staining pink even though the patient tissue is staining beautifully on the same slide. The stain is done manually. What can be some causes for the issues in staining on the control section? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From jwwalker at rrmc.org Fri Oct 9 09:30:17 2020 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Fri, 9 Oct 2020 14:30:17 +0000 Subject: [Histonet] CAP required PT In-Reply-To: References: Message-ID: Any IHC that involves prognostic indications requires PT. A short list would include ER, PgR, HER2 but there are others. CAP info is here: https://documents.cap.org/documents/2021-Surveys-catalog-mobile.pdf Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology and Interim Phlebotomy Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Wood, Barry A via Histonet Sent: Thursday, October 8, 2020 2:25 PM To: 'Histonet at lists.utsouthwestern.edu' Subject: [Histonet] CAP required PT [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. For which IHC stains is it required by CAP to do proficiency testing? Barry Wood CT (ASCP) Director of Anatomic Pathology Indiana University Health 317-491-6351 bwood at iuhealth.org Compassionate. Highly skilled. Personalized. Visit iuhealth.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!I87qwjxLstg3H_X5!o2p8HxfxYtHkICrMPsW71rrdvq29jkFPVjiIz2MYp-cAVjHZ0nDKgGzWV_W8vUw$ [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] From jwwalker at rrmc.org Fri Oct 9 11:15:52 2020 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Fri, 9 Oct 2020 16:15:52 +0000 Subject: [Histonet] CAP required PT In-Reply-To: <9FEE1750-09E9-41F7-A325-4CADC5BA3523@gmail.com> References: <9FEE1750-09E9-41F7-A325-4CADC5BA3523@gmail.com> Message-ID: In the June 2020 Checklists, it has been moved to the Commons as COM.01520 Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology and Interim Phlebotomy Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790? F 802.747.6525 joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Garrey Faller Sent: Friday, October 9, 2020 11:35 AM To: Joe W. Walker, Jr. Subject: Re: [Histonet] CAP required PT [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. Hi joe, Can you point me to the cap reg for this? I can?t find it in the ap checklist. Is it in all common? Thanks. Garrey Sent from my iPhone > On Oct 9, 2020, at 10:49 AM, Joe W. Walker, Jr. via Histonet wrote: > > ?Any IHC that involves prognostic indications requires PT. A short > list would include ER, PgR, HER2 but there are others. CAP info is > here: > https://urldefense.com/v3/__https://documents.cap.org/documents/2021-S > urveys-catalog-mobile.pdf__;!!I87qwjxLstg3H_X5!v4MZvbrMCG7Y_tKHna_HwzE > HdcLZmuhYM0UllgXIW6tQcPbpGothba-dAsQAxd8$ > > Joe W. Walker, Jr. MS, SCT(ASCP) > Anatomical Pathology and Interim Phlebotomy Manager Rutland Regional > Medical Center > 160 Allen Street, Rutland, VT 05701 > P 802.747.1790 F 802.747.6525 > joewalker at rrmc.org, http://www.rrmc.org > > -----Original Message----- > From: Wood, Barry A via Histonet > Sent: Thursday, October 8, 2020 2:25 PM > To: 'Histonet at lists.utsouthwestern.edu' > > Subject: [Histonet] CAP required PT > > [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. > > > For which IHC stains is it required by CAP to do proficiency testing? > > Barry Wood CT (ASCP) > Director of Anatomic Pathology > Indiana University Health > 317-491-6351 > bwood at iuhealth.org > Compassionate. Highly skilled. Personalized. Visit iuhealth.org > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li > stinfo/histonet__;!!I87qwjxLstg3H_X5!o2p8HxfxYtHkICrMPsW71rrdvq29jkFPV > jiIz2MYp-cAVjHZ0nDKgGzWV_W8vUw$ > [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_fin > al.jpg] _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li > stinfo/histonet__;!!I87qwjxLstg3H_X5!v4MZvbrMCG7Y_tKHna_HwzEHdcLZmuhYM > 0UllgXIW6tQcPbpGothba-dTYA_vig$ From jkiernan at uwo.ca Sat Oct 10 12:32:00 2020 From: jkiernan at uwo.ca (John Kiernan) Date: Sat, 10 Oct 2020 17:32:00 +0000 Subject: [Histonet] Alcian Blue staining In-Reply-To: References: Message-ID: Pink with alcian blue? Are you using an alcian blue pH2.5-PAS sequence? If so you should get PAS-positive mucus (not in goblet cells) in the stomach and AB-positive mucus (greenish blue) in the intestine. Intestinal mucus, especially in the duodenum and jejunum, is also PAS +ve, so the cells appear purplish. In the colon the mucus is mostly sulphated rather than sialylated, so it is only AB +ve, even if the stain is done at pH 1. If a PAS stain is done before the AB, PAS +ve mucus also stains quite strongly with AB. For more about this, see Johannes ML & Klessen C (1984) Alcianblue/PAS or PAS/alcianblue. Remarks on a classical technic used in carbohydrate histochemistry. Histochemistry 80: 129-132. (Unfortunately the paper has only black & white photos.) Various dyes are currently sold as "alcian blue" and not all are suitable for all applications. The Biological Stain Commission now certifies alcian blue dyes as either "alcian blue 8G or equivalent" or as "alcian blue variant". See the recently updated (2020) entry for Alcian blue 8G (CI 74240) and other alcian blue dyes at https://biologicalstaincommission.org/new-dyes/. Current issues of interest to vendors and users of dyes and biological stains. | The Biological Stain Commission Dyes are becoming more expensive! January 2018. BASF, a major dyestuff manufacturing company, recently announced that it has increased its prices for many pigments and dyes by up to 15% worldwide. biologicalstaincommission.org John Kiernan Secretary. Biological Stain Commission = = = ________________________________ From: Charles Riley via Histonet Sent: October 9, 2020 10:14 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Alcian Blue staining We are having an issue where the goblet cells in our control tissue are not staining pink even though the patient tissue is staining beautifully on the same slide. The stain is done manually. What can be some causes for the issues in staining on the control section? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt at pa-ucl.com Sun Oct 11 10:34:07 2020 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Sun, 11 Oct 2020 15:34:07 +0000 Subject: [Histonet] AFB control and Recycling formalin Message-ID: Hello- 1. Does anyone have extra AFB control that we could obtain some from? 1. Recycling formalin: if you are recycling formalin, do you recycle for any other sites? * How do you charge for this? * Do you charge for what is coming in or what is going out? Working through a multi-site dissolution and trying to future plan...... Thoughts appreciated! Nancy Schmitt HT, MLT(ASCP) Pathology Support Services Manager United Clinical Laboratories 250 Mercy Drive Dubuque, IA 52001 Ph 563-589-9810 From 11z at comcast.net Sun Oct 11 17:45:10 2020 From: 11z at comcast.net (11z at comcast.net) Date: Sun, 11 Oct 2020 15:45:10 -0700 Subject: [Histonet] hexamethyleneimine Message-ID: <000001d6a020$2da2a600$88e7f200$@comcast.net> What do you use hexamethyleneimine for in your lab. I found an old bottle of this reagent and cannot recall why I have it. It must be used in making up a stain but I am not remembering what stain. Anyone know? Thanks LeRoy Brown HT(ASCP)HTL From john.garratt at ciqc.ca Sun Oct 11 17:58:22 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Sun, 11 Oct 2020 22:58:22 +0000 Subject: [Histonet] hexamethyleneimine In-Reply-To: <000001d6a020$2da2a600$88e7f200$@comcast.net> References: <000001d6a020$2da2a600$88e7f200$@comcast.net> Message-ID: It is better known as Hexamine in histo circles. Gomori Hexamine Silver, for example, can be used to nicely demonstrate Glomeruli. John On Sun, Oct 11, 2020 at 3:45 PM, LEROY H BROWN via Histonet wrote: > What do you use hexamethyleneimine for in your lab. I found an old bottle > of this reagent and cannot recall why I have it. > > It must be used in making up a stain but I am not remembering what stain. > > Anyone know? > > Thanks > > LeRoy Brown HT(ASCP)HTL > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Sun Oct 11 18:15:44 2020 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun, 11 Oct 2020 23:15:44 +0000 Subject: [Histonet] hexamethyleneimine In-Reply-To: References: <000001d6a020$2da2a600$88e7f200$@comcast.net> Message-ID: <134d1cd6c0ac489d88a179503ca31a51@SVDCMBX-MEX023.nswhealth.net> I agree Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: John Garratt via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, 12 October 2020 9:58 AM To: 11z at comcast.net; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] hexamethyleneimine It is better known as Hexamine in histo circles. Gomori Hexamine Silver, for example, can be used to nicely demonstrate Glomeruli. John On Sun, Oct 11, 2020 at 3:45 PM, LEROY H BROWN via Histonet wrote: > What do you use hexamethyleneimine for in your lab. I found an old > bottle of this reagent and cannot recall why I have it. > > It must be used in making up a stain but I am not remembering what stain. > > Anyone know? > > Thanks > > LeRoy Brown HT(ASCP)HTL > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From gu.lang at gmx.at Tue Oct 13 01:57:48 2020 From: gu.lang at gmx.at (Gudrun Lang) Date: Tue, 13 Oct 2020 08:57:48 +0200 Subject: [Histonet] hexamethyleneimine In-Reply-To: <000001d6a020$2da2a600$88e7f200$@comcast.net> References: <000001d6a020$2da2a600$88e7f200$@comcast.net> Message-ID: <000901d6a12e$2bba3740$832ea5c0$@gmx.at> Jones Methenamin Silver Impregnation, for staining basalmembrans in glomeruli. Gudrun -----Urspr?ngliche Nachricht----- Von: LEROY H BROWN via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Montag, 12. Oktober 2020 00:45 An: histonet at lists.utsouthwestern.edu Betreff: Re: [Histonet] hexamethyleneimine What do you use hexamethyleneimine for in your lab. I found an old bottle of this reagent and cannot recall why I have it. It must be used in making up a stain but I am not remembering what stain. Anyone know? Thanks LeRoy Brown HT(ASCP)HTL _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Tue Oct 13 09:35:59 2020 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 13 Oct 2020 10:35:59 -0400 Subject: [Histonet] Histology Supervisor needed in Florida! Can you help? Message-ID: <006301d6a16e$2b874c50$8295e4f0$@earthlink.net> Hi Histopeeps, How are you? I hope you are having a great week! I have several exciting opportunities to share and if you aren't interested maybe you know someone who might be! I have been engaged on searches by several clients located in the state of Florida. Here is the info: * Tampa Bay - Histology Supervisor - Days * Tampa Bay - Lead Histology Tech - Days The help I need from you Histopeeps is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them or pass their contact information to me? *remember if I place someone you refer to me you earn a referral bonus* Both of these positions are full time and permanent. And my clients are ready to interview and hire!! If you are interested in this position please contact me ASAP On my cell/text 407-353-5070 or toll free at 866-607-3542 Or via email at relia1 at earthlink.net If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won't release it to anyone without your permission. Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From brettmc31 at comcast.net Tue Oct 13 13:19:51 2020 From: brettmc31 at comcast.net (Brett Connolly) Date: Tue, 13 Oct 2020 14:19:51 -0400 Subject: [Histonet] Old Tissue Tek processor Message-ID: Does anyone have a pic of the old style Fisher Tissue Tek Processor?...the one that was popular in the early 1980s. I couldn?t find any on-line. Thanks. Brett Connolly, Phd, HTL(ASCP) Sent from Mail for Windows 10 From paula at excaliburpathology.com Tue Oct 13 14:05:37 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Tue, 13 Oct 2020 19:05:37 +0000 (UTC) Subject: [Histonet] Old Tissue Tek processor In-Reply-To: References: Message-ID: <178332677.200087.1602615937590@mail.yahoo.com> There is one for sale on ebay. https://www.ebay.com/itm/FISHER-HISTOMATIC-TISSUE-PROCESSOR-MODEL-166-MP/151784782912?hash=item235713fc40:g:CbkAAOSwgQ9V02Xl Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Tuesday, October 13, 2020, 01:33:49 PM CDT, Brett Connolly via Histonet wrote: Does anyone have a pic of the old style Fisher Tissue Tek Processor?...the one that was popular in the early 1980s. I couldn?t find any on-line. Thanks. Brett Connolly, Phd, HTL(ASCP) Sent from Mail for Windows 10 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brettmc31 at comcast.net Tue Oct 13 14:30:16 2020 From: brettmc31 at comcast.net (Brett Connolly) Date: Tue, 13 Oct 2020 15:30:16 -0400 Subject: [Histonet] Old Tissue Tek processor In-Reply-To: <178332677.200087.1602615937590@mail.yahoo.com> References: <178332677.200087.1602615937590@mail.yahoo.com> Message-ID: Thanks Paula, That?s pretty much the one I was looking for and what I first trained on. What a freakin? dinosaur!! Brett Sent from Mail for Windows 10 From: Paula Keene Pierce Sent: Tuesday, October 13, 2020 3:05 PM To: histonet at lists.utsouthwestern.edu; Brett Connolly Subject: Re: [Histonet] Old Tissue Tek processor There is one for sale on ebay. https://www.ebay.com/itm/FISHER-HISTOMATIC-TISSUE-PROCESSOR-MODEL-166-MP/151784782912?hash=item235713fc40:g:CbkAAOSwgQ9V02Xl Paula Keene Pierce, BS, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Drive Norman, OK 73069 PH 405-759-3953 http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Tuesday, October 13, 2020, 01:33:49 PM CDT, Brett Connolly via Histonet wrote: Does anyone have a pic of the old style Fisher Tissue Tek Processor?...the one that was popular in the early 1980s. I couldn?t find any on-line. Thanks. Brett Connolly, Phd, HTL(ASCP) Sent from Mail for Windows 10 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff at uropartners.com Thu Oct 15 07:34:52 2020 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 15 Oct 2020 12:34:52 +0000 Subject: [Histonet] For those of you who have cut autopsy slides (blog post) Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF175BE528@COLOEXCH01.uropartners.local> Hope all of you are doing well. http://www.chicagonow.com/downsize-maybe/2020/10/brain-cutting/ Lester J. Raff, MD MBA FCAP Laboratory Director UroPartners LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 Telephone 708-486-0076 Fax 708-492-0203 From jsagasser at gandhigi.com Thu Oct 15 11:46:32 2020 From: jsagasser at gandhigi.com (Jacque Sagasser) Date: Thu, 15 Oct 2020 16:46:32 +0000 Subject: [Histonet] Controls Message-ID: Good afternoon! Could any of you tell me off hand how old patient tissue has to be before you can use it as a control? My lab has only been operating since February. We run quite a few Alcian Blue PAS stains a week, and my last control is getting sparse. I have some beautiful patient tissue that I could use if the time limit has been exceeded. I am trying to avoid purchasing controls because we are a small GI lab, and that eats into our operating costs. That said, if tissue has to be older than the amount of time our lab has been open, does anyone have a spare control they could pass on to me please? I would be willing to assist you with anything I am able to assist with in the future. ?Jacque R. Sagasser, HT (ASCP)cm Gandhi GI Pathology, LLC 999 Brubaker Drive Suite 1 Kettering OH 45429 jsagasser at gandhigi.com 937-795-1099 phone 937-519-1321 fax From Kelly.Pairan at nationwidechildrens.org Thu Oct 15 12:35:05 2020 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Thu, 15 Oct 2020 17:35:05 +0000 Subject: [Histonet] Evaporation in frozen aliquots Message-ID: Good Afternoon, Do any of your labs aliquot antibody concentrates and store them at -20? Do you experience evaporation? Thanks for your help, Kelly From Richard.Cartun at hhchealth.org Thu Oct 15 13:15:56 2020 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Thu, 15 Oct 2020 18:15:56 +0000 Subject: [Histonet] Controls In-Reply-To: References: Message-ID: I would recommend waiting at least 2 weeks after the case is signed-out in case additional testing needs to be performed, especially if the specimen contains cancer. And, I would always make sure that you leave representative tissue in the block meaning don't consume the entire specimen when you cut your control sections unless the case has multiple blocks showing the same pathology. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org -----Original Message----- From: Jacque Sagasser via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, October 15, 2020 12:47 PM To: 'Histonet (histonet at lists.utsouthwestern.edu)' Subject: [Histonet] Controls STOP, THINK, READ. This is an external email. Exercise extra caution responding to it, opening attachments and following links. Good afternoon! Could any of you tell me off hand how old patient tissue has to be before you can use it as a control? My lab has only been operating since February. We run quite a few Alcian Blue PAS stains a week, and my last control is getting sparse. I have some beautiful patient tissue that I could use if the time limit has been exceeded. I am trying to avoid purchasing controls because we are a small GI lab, and that eats into our operating costs. That said, if tissue has to be older than the amount of time our lab has been open, does anyone have a spare control they could pass on to me please? I would be willing to assist you with anything I am able to assist with in the future. ?Jacque R. Sagasser, HT (ASCP)cm Gandhi GI Pathology, LLC 999 Brubaker Drive Suite 1 Kettering OH 45429 jsagasser at gandhigi.com 937-795-1099 phone 937-519-1321 fax _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!JE_DSMiJJOIqcoBfBHt09qvO0We4-zpHEADQfbNIr282bdD0Xju5hESmd9xhVlW2bMo$ Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From carl.hobbs at kcl.ac.uk Fri Oct 16 12:39:56 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 16 Oct 2020 17:39:56 +0000 Subject: [Histonet] Evaporation in frozen aliquots Message-ID: Hi Kelly If the tubes that hold your frozen ab aliquots do not have a complete seal....the water in them will lyophilise, over time. I have your problem occasionally, for that reason ( I have a couple of thousand frozen primary abs) Use snap-lid tubes ( I use 200microL PCR tubes) Hope that explains. Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From katherine at ka-recruiting.com Wed Oct 21 17:07:54 2020 From: katherine at ka-recruiting.com (Katherine Marano) Date: Wed, 21 Oct 2020 18:07:54 -0400 Subject: [Histonet] Hiring Histotechs Message-ID: Hi Histonetters, I am working with a facility in Atlanta, Georgia looking to hire an ASCP certified Histotech and/or grossing tech to work on their day shift, Monday-Friday. These are permanent positions with fantastic benefits including PTO, retirement plans, relocation assistance. I would love to give you more information if you are interested? Let me know! I also have some other permanent and full-time histology positions available ? Northern Florida - Histotech (3:30p-Midnight) Western Florida - Histology Manager (5 years histology supervisory experience required) Atlanta, Georgia - Histology Supervisor Atlanta, Georgia - Histotech (1st shift) Atlanta, Georgia - Grossing Tech (1st shift) Central Georgia - Histotech (3a-11:30am) Northwest Illinois - Histotech NYC - Grossing Tech (evenings) NYC - Histology Supervisor (evenings, 3p-1130p ) NYC - SurgPath (IHC) CLT (evenings) Westchester County, NY - Lead IHC Tech (must have tissue cutting exp) Westchester County, NY - Grossing Supervisor Syracuse, NY - Histotech (1st or 2nd shift) Long Island, NY - Histotechnician or Histotechnologist Central Texas - Histology Supervisor Southern Virginia - Histotech (rotating shift) Eastern Wisconsin - Histotech (2nd shift) Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From rmccormick10 at yahoo.com Thu Oct 22 14:30:11 2020 From: rmccormick10 at yahoo.com (Rhonda McCormick) Date: Thu, 22 Oct 2020 19:30:11 +0000 (UTC) Subject: [Histonet] MelanA References: <1986462356.2444984.1603395011208.ref@mail.yahoo.com> Message-ID: <1986462356.2444984.1603395011208@mail.yahoo.com> I thought I'd reach out and see if anyone can help with some IHC troubleshooting (or know someone who might). Our Melan A that once was working on our canine tissue, no longer is. We are using: 1:300 dilution of the Leica MelA antibody BioCare IHC platform Antigen Retrieval performed in pressure cooker Alkaline Phosphatase detection We are also using known controls and still... no staining. We'd appreciate any ideas or recommendations (or even questions that might lead to those "lightbulb" moments). Sometimes when you're so mired in the problem, it just takes someone else to shed some light on it. Thank you Rhonda From cforster at umn.edu Thu Oct 22 15:30:29 2020 From: cforster at umn.edu (Colleen Forster) Date: Thu, 22 Oct 2020 15:30:29 -0500 Subject: [Histonet] MelanA In-Reply-To: <1986462356.2444984.1603395011208@mail.yahoo.com> References: <1986462356.2444984.1603395011208.ref@mail.yahoo.com> <1986462356.2444984.1603395011208@mail.yahoo.com> Message-ID: Rhinda, Which Alkaline Phos detection? How old is the kit? I have found that the Alk Phos detection can die before the stated out date. I use a Biocare platform as well. I have learned to leave the chromagen 4 degrees until I am ready to stain. I make what I need and immediately [lace the whole kit back into 4 degrees. Just one thought. Colleen Forster HT(ASCP)QIHC U of MN On Thu, Oct 22, 2020 at 2:30 PM Rhonda McCormick via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I thought I'd reach out and see if anyone can help with some IHC > troubleshooting (or know someone who might). Our Melan A that once was > working on our canine tissue, no longer is. > > We are using: > > 1:300 dilution of the Leica MelA antibody > > BioCare IHC platform > > Antigen Retrieval performed in pressure cooker > > Alkaline Phosphatase detection > > > We are also using known controls and still... no staining. > > We'd appreciate any ideas or recommendations (or even questions that might > lead to those "lightbulb" moments). Sometimes when you're so mired in the > problem, it just takes someone else to shed some light on it. > > > Thank you > > > Rhonda > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 612-626-1930 From kimberly.glover at polysciences.com Fri Oct 23 13:52:01 2020 From: kimberly.glover at polysciences.com (Glover, Kimberly) Date: Fri, 23 Oct 2020 18:52:01 +0000 Subject: [Histonet] CLIA classification Message-ID: Does anyone know if Quick III stain or Diff Quick classifies as waiver, moderately, or highly complex per CLIA/FDA? Kim From eddessa at emory.edu Fri Oct 23 14:13:01 2020 From: eddessa at emory.edu (Dessasau, Evan D.) Date: Fri, 23 Oct 2020 19:13:01 +0000 Subject: [Histonet] [External] Re: hexamethyleneimine In-Reply-To: <000001d6a020$2da2a600$88e7f200$@comcast.net> References: <000001d6a020$2da2a600$88e7f200$@comcast.net> Message-ID: Hi LeRoy, we use it in the GMS stain. It is the Methenamine solution. Thank you, E-van Yerkes National Primate Research Center Emory University 954 Gatewood Road Atlanta, GA 30329, USA (404) 727-7744 (on campus 7-7744) Lab eddessa at emory.edu -----Original Message----- From: LEROY H BROWN via Histonet Sent: Sunday, October 11, 2020 6:45 PM To: histonet at lists.utsouthwestern.edu Subject: [External] Re: [Histonet] hexamethyleneimine What do you use hexamethyleneimine for in your lab. I found an old bottle of this reagent and cannot recall why I have it. It must be used in making up a stain but I am not remembering what stain. Anyone know? Thanks LeRoy Brown HT(ASCP)HTL _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=04%7C01%7Ceddessa%40emory.edu%7Cb74f0fc987e34435753108d86e376493%7Ce004fb9cb0a4424fbcd0322606d5df38%7C0%7C0%7C637380531580544639%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000&sdata=2rE0it4LwJpJEq%2BCyId4XSgpOOAIEXRbOmU3GyO5cfI%3D&reserved=0 ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From ASelf at tidelandshealth.org Mon Oct 26 07:28:35 2020 From: ASelf at tidelandshealth.org (Amy Self) Date: Mon, 26 Oct 2020 12:28:35 +0000 Subject: [Histonet] Non-Pathologist Grossing Message-ID: Good Morning and I hope that everyone had a wonderful, safe and blessed weekend, I have a question about non-pathologist grossing review.. We were inspected by TJC week before last and were cited for the Standard QSA.13.04.01 which reads; When a non-pathologist performs gross analysis under the supervision of a qualified pathologist; The individuals work is reviewed by the technical supervisor or qualified pathologist within 24 hours and this review is documented. How is this 24 hour review being performed by facilities that are closed on weekends and holidays? Our departments review would take place on the next business day - being Monday - but the inspector said that was unacceptable because it did not fall within the 24 hour review stated in the standard. Thanks in advance and I hope that everyone has a great Monday, Amy Self Senior Histology Technologist Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 Office: (843) 520-8711 aself at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From bakevictoria at gmail.com Mon Oct 26 08:33:05 2020 From: bakevictoria at gmail.com (Victoria Baker) Date: Mon, 26 Oct 2020 09:33:05 -0400 Subject: [Histonet] Frozen section QA Message-ID: Happy Monday morning! We recently had an incident which led us to review our policy on frozen section QA. In your institution does the same pathologist who did the frozen section read the permanent sections? Also if they do then who is the pathologist responsible for doing the QA? How is this work flow completed? Last, does any facility with less than 450 beds have their pathologists specialized. Thank you in advance for your help. Have a wonderful week ahead! Vikki Baker From rsrichmond at gmail.com Mon Oct 26 14:02:05 2020 From: rsrichmond at gmail.com (Bob Richmond) Date: Mon, 26 Oct 2020 15:02:05 -0400 Subject: [Histonet] Non-Pathologist Grossing (Amy Self) In-Reply-To: References: Message-ID: Vikki Baker (or Amy self) - where? - asks: > >>We recently had an incident which led us to review our policy on frozen > section QA. In your institution does the same pathologist who did the > frozen section read the permanent [paraffin] sections? Also if they do > then who is the pathologist responsible for doing the QA? > Last, does any facility with less than 450 beds have their pathologists specialized?<< This is a very serious question that should be decided by pathologists. It's a pretty basic principle in pathology practice that whenever possible (and it often isn't possible) a case should be the responsibility of a single pathologist from start to finish. A pathologist is a physician, not a technician (though this is news to a lot of people), and is not interchangeable in the care of a single patient. The most common problem here is the case with multiple frozen sections done on margins of resection. Often the initial record of where various sections came from is unclear, and only the responsible pathologist can straighten the record out in the final sign-out. As to the second question, most younger pathologists practicing general pathology have subspecialty qualifications. In any pathology group, cases in a pathologist's subspecialty will often be seen by that subspecialist. Finally, "paraffin sections", not "permanent sections". At the age of 81, I'm just old enough to remember the old "wet knife" frozen section technique, that resulted in sections set loosely on the slide, and not easily preserved. During the 1960s the cryostat replaced the old method. Correctly prepared frozen section slides are quite as permanent as the subsequent paraffin sections. Bob Richmond Samurai Pathologist Maryville TN > > From relia1 at earthlink.net Wed Oct 28 10:42:26 2020 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 28 Oct 2020 11:42:26 -0400 Subject: [Histonet] Florida Licensure Required Courses Message-ID: <00d701d6ad40$efb00210$cf100630$@earthlink.net> Hi Histopeeps! I hope you are having a great day. Could someone please send me a link to the required courses for Florida licensure? I have a candidate that needs to take the courses. Thanks in advance. Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From redrose297 at gmail.com Wed Oct 28 13:48:14 2020 From: redrose297 at gmail.com (warda hassan) Date: Wed, 28 Oct 2020 22:48:14 +0400 Subject: [Histonet] Selection of IHC system Message-ID: Dear Histonet Our lab is looking to purchase a new IHC system. Currently we are working with Dako. I would request your valuable feedback on :- 1- Bond max ( Leica) 2- Bench Mark Ultra (Roche Diagnostic) Workload is 15,000-20,000 slides/year. Many thanks in advance for your help. Kind regards W.A Hassan From tina.vanmeter at gmail.com Wed Oct 28 22:08:07 2020 From: tina.vanmeter at gmail.com (Tina Van Meter) Date: Wed, 28 Oct 2020 23:08:07 -0400 Subject: [Histonet] Selection of IHC system In-Reply-To: References: Message-ID: Bond Max On Wed, Oct 28, 2020, 2:53 PM warda hassan via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Dear Histonet > > Our lab is looking to purchase a new IHC system. > Currently we are working with Dako. > I would request your valuable feedback on :- > > 1- Bond max ( Leica) > 2- Bench Mark Ultra (Roche Diagnostic) > > Workload is 15,000-20,000 slides/year. > > Many thanks in advance for your help. > > Kind regards > W.A Hassan > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From edmartin26 at gmail.com Thu Oct 29 16:12:43 2020 From: edmartin26 at gmail.com (Eddie Martin) Date: Thu, 29 Oct 2020 17:12:43 -0400 Subject: [Histonet] Histonet Digest, Vol 203, Issue 21 In-Reply-To: References: Message-ID: Hi Histonet and W.A. Hassan, Supposing you have a slide volume of 15-20k slides per year & currently use a Dako instrument, but would like feedback on whether to get a: * Bond Max, or * Roche Ultra My response would depend more than what your yearly slide volume is. * If...continually loading of slides while having access to the reagent carousel is important to you, then I'd say Roche Ultra has the advantage. *If...having more control of your IHC protocols to change your retrieval conditions, or even loading one time retrieval conditions without saving it as a new protocol, then I'd say Leica Bond has the advantage. It really depends on what your lab will be staining is what I'm getting to. Another factor to include is ease of use. Roche Ultra is literally a lot easier to use than Leica Bond, and Leica Bond is easier to use than the old Dako's or Dako Flex or similarly Thermo or Biocare IHC instruments. * If you're staining odd protocols and want an instrument staining biomarker assays at variant conditions outside the norm: (ie: staining 50-100 micron frozen sections looking for nerve bundles, or trying to perform FISH protocols on the instrument without an installed FISH protocol on the instrument), then Leica is the choice. quasi molecular staining: 1. if your lab is interested in using m-RNA oligo probes, choose Leica, as they have an easier setup to using different vendors other than their own mRNA detection. 2. if your physician staff prefers to see probes with a matte background, only showing positive probe staining (though using an older, yet very robust detection method), then Roche Ultra has the advantage. If you're at a reference Lab and molecular performing testing that used to be molecular based, but is now available via IHC, then Roche Ultra has a huge advantage, particularly for the therapeutic and theranostic biomarkers commercially available. Other factors to consider in your choice: *Cost * footprint of the instrument in your lab * disposing of waste * daily, weekly & monthly maintenance of the instrument * cost of service. Using a third party biomedical group to repair or PM the instrument removes the IVD status of the instrument, especially if you're staining any Class II's or higher, like CD117, or ER, PR, Her2Neu...etc. ***Its ok if you're just using it for research if this doesn't apply. I hope this list helps you think what your team would like to use. if your pathologist and additional primary stakeholders would like to contact me... you can. I previously was a application specialist for IHC/probes with Leica Microsystems up to 8 years ago. However, I use a lot of Roche family of instruments in my lab, as I'm a contracted lead for a well known anatomic/clinical pathology laboratory. Best regards, Eddie Martin, HT, HTL, QIHC On Thu, Oct 29, 2020 at 1:00 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Selection of IHC system (warda hassan) > 2. Re: Selection of IHC system (Tina Van Meter) > > > > ---------- Forwarded message ---------- > From: warda hassan > To: "histonet at lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Wed, 28 Oct 2020 22:48:14 +0400 > Subject: [Histonet] Selection of IHC system > Dear Histonet > > Our lab is looking to purchase a new IHC system. > Currently we are working with Dako. > I would request your valuable feedback on :- > > 1- Bond max ( Leica) > 2- Bench Mark Ultra (Roche Diagnostic) > > Workload is 15,000-20,000 slides/year. > > Many thanks in advance for your help. > > Kind regards > W.A Hassan > > > > > ---------- Forwarded message ---------- > From: Tina Van Meter > To: warda hassan > Cc: Histonet at lists.utsouthwestern.edu > Bcc: > Date: Wed, 28 Oct 2020 23:08:07 -0400 > Subject: Re: [Histonet] Selection of IHC system > Bond Max > > On Wed, Oct 28, 2020, 2:53 PM warda hassan via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Dear Histonet > > > > Our lab is looking to purchase a new IHC system. > > Currently we are working with Dako. > > I would request your valuable feedback on :- > > > > 1- Bond max ( Leica) > > 2- Bench Mark Ultra (Roche Diagnostic) > > > > Workload is 15,000-20,000 slides/year. > > > > Many thanks in advance for your help. > > > > Kind regards > > W.A Hassan > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Bacon at baystatehealth.org Fri Oct 30 07:34:30 2020 From: Charles.Bacon at baystatehealth.org (Bacon, Charles) Date: Fri, 30 Oct 2020 12:34:30 +0000 Subject: [Histonet] Ventana Benchmark Ultra Message-ID: <3cc82d6084864ecc81db64bac7e0a25a@ZXSWEXCHMXPR01.bhs.org> Hello Warda, As you know the Dako Autostainer and PT link set up works well but has a good amount of tech time involved in its use. We currently run the Dako as well as the Benchmark Ultra. The Ultra is a major upgrade in ease of use. The antigen retrieval is onboard with no tech time to switch and load after that step, like on the Dako. Our pathologist do still like specific antibodies run on the Dako platform but that is getting very specific with antibodies. They all agree they work on the Ultra but "like it better on the Dako." To me it comes down to open or closed. With the Ventana we use mostly prediluted antibodies with some exceptions where we make a prep kit. On the Dako which we have had for years longer than the Ventana, there was more room for developing the specific nuance of a given antibody. We seem to be able to tweak the settings on the Dako more to our pathologist liking. In summation the Dako is a bit more open but to me you cannot weight that against tech time and TAT of the Benchmark. We run the PD-L1 developed by Dako so we will always need that platform, but I prefer the Ventana equipment overall. Best, Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786? Fax: 413-322-4790 Charles.Bacon at baystatehealth.org -----Original Message----- From: warda hassan Sent: Wednesday, October 28, 2020 2:48 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Selection of IHC system Dear Histonet Our lab is looking to purchase a new IHC system. Currently we are working with Dako. I would request your valuable feedback on :- 1- Bond max ( Leica) 2- Bench Mark Ultra (Roche Diagnostic) Workload is 15,000-20,000 slides/year. Many thanks in advance for your help. Kind regards W.A Hassan ---------------------------------------------------------------------- Please view our annual report at http://www.bhannualreport.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at 413-794-0000 and destroy all copies of this communication and any attachments. For further information regarding Baystate Health's privacy policy, please visit our Internet site at https://www.baystatehealth.org. From jhill at vet.k-state.edu Fri Oct 30 10:45:26 2020 From: jhill at vet.k-state.edu (Jennifer Phinney) Date: Fri, 30 Oct 2020 15:45:26 +0000 Subject: [Histonet] Crab histology Message-ID: Hello Histopeeps, Does anyone have experience with histology on crustaceans? My lab received a moon crab with shell lesions (of course) and I'm wondering what the best treatment is to ensure the lab can cut the best slides of the shell possible. I am currently thinking a combination of decal and Nair to deal with the chitin and calcium carbonate that makes up the shell. I have an insect histology book that details other ways to soften chitin, but this is on a larger scale than the small insects my lab has dealt with before. Thanks for any guidance, Jennifer Phinney MS QIHC Histology Laboratory Administrator Kansas State Veterinary Diagnostic Lab From edmartin26 at gmail.com Fri Oct 30 14:21:12 2020 From: edmartin26 at gmail.com (Eddie Martin) Date: Fri, 30 Oct 2020 15:21:12 -0400 Subject: [Histonet] Vol 203, Issue 21 In-Reply-To: References: Message-ID: Hi Warda, 1. Both Roche and Leica have different instruments that are both equally capable of breaking down. It's really not feasible to determine which will cost more in repairs on this premise alone. 2. Roche Ultra stains their probes with either a blue chromogen or you may also use a Silver chromogen. I hope this helps! Eddie Martin On Fri, Oct 30, 2020 at 3:30 AM warda hassan wrote: > Dear Eddie > > Thank you so much for your detailed explanation. > Its really helpful, I just had two questions since you had worked on both > the systems > > 1- Which one of them requires more maintenance and causes more breakdowns. > > 2- Does Roche performs FISH or is it SISH with probe IHC principle > > Thank you so much > W Hassan > > On Fri, 30 Oct 2020 at 1:12 AM Eddie Martin wrote: > >> Hi Histonet and W.A. Hassan, >> >> Supposing you have a slide volume of 15-20k slides per year & currently >> use a Dako instrument, but would like feedback on whether to get a: >> * Bond Max, or >> * Roche Ultra >> >> My response would depend more than what your yearly slide volume is. >> * If...continually loading of slides while having >> access to the reagent carousel is important to you, then I'd say Roche >> Ultra has the advantage. >> *If...having more control of your IHC protocols to >> change your retrieval conditions, or even loading one time retrieval >> conditions without saving it as a new protocol, then I'd say Leica Bond has >> the advantage. >> >> It really depends on what your lab will be staining is what I'm getting >> to. >> Another factor to include is ease of use. Roche Ultra is literally a lot >> easier to use than Leica Bond, and Leica Bond is easier to use than the old >> Dako's or Dako Flex or similarly Thermo or Biocare IHC instruments. >> * If you're staining odd protocols and want an >> instrument staining biomarker assays at variant conditions outside the >> norm: >> (ie: staining 50-100 micron frozen sections looking >> for nerve bundles, or trying to perform FISH protocols on the instrument >> without an installed FISH protocol on the instrument), then Leica is the >> choice. >> >> quasi molecular staining: >> 1. if your lab is interested in using m-RNA >> oligo probes, choose Leica, as they have an easier setup to using different >> vendors other than their own mRNA detection. >> 2. if your physician staff prefers to see >> probes with a matte background, only showing positive probe staining >> (though using an older, yet very robust detection method), then Roche Ultra >> has the advantage. >> >> If you're at a reference Lab and molecular performing testing that used >> to be molecular based, but is now available via IHC, then Roche Ultra has a >> huge advantage, particularly for the therapeutic and theranostic biomarkers >> commercially available. >> >> Other factors to consider in your choice: >> *Cost >> * footprint of the instrument in your lab >> * disposing of waste >> * daily, weekly & monthly maintenance of the instrument >> * cost of service. Using a third party biomedical >> group to repair or PM the instrument removes the IVD status of the >> instrument, especially if you're staining any Class II's or higher, like >> CD117, or ER, PR, Her2Neu...etc. >> ***Its ok if you're just >> using it for research if this doesn't apply. >> >> I hope this list helps you think what your team would like to use. if >> your pathologist and additional primary stakeholders would like to contact >> me... you can. >> I previously was a application specialist for IHC/probes with Leica >> Microsystems up to 8 years ago. However, I use a lot of Roche family of >> instruments in my lab, as I'm a contracted lead for a well known >> anatomic/clinical pathology laboratory. >> >> Best regards, >> Eddie Martin, HT, HTL, QIHC >> >> On Thu, Oct 29, 2020 at 1:00 PM < >> histonet-request at lists.utsouthwestern.edu> wrote: >> >>> Send Histonet mailing list submissions to >>> histonet at lists.utsouthwestern.edu >>> >>> To subscribe or unsubscribe via the World Wide Web, visit >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> or, via email, send a message with subject or body 'help' to >>> histonet-request at lists.utsouthwestern.edu >>> >>> You can reach the person managing the list at >>> histonet-owner at lists.utsouthwestern.edu >>> >>> When replying, please edit your Subject line so it is more specific >>> than "Re: Contents of Histonet digest..." >>> Today's Topics: >>> >>> 1. Selection of IHC system (warda hassan) >>> 2. Re: Selection of IHC system (Tina Van Meter) >>> >>> >>> >>> ---------- Forwarded message ---------- >>> From: warda hassan >>> To: "histonet at lists.utsouthwestern.edu" < >>> histonet at lists.utsouthwestern.edu> >>> Cc: >>> Bcc: >>> Date: Wed, 28 Oct 2020 22:48:14 +0400 >>> Subject: [Histonet] Selection of IHC system >>> Dear Histonet >>> >>> Our lab is looking to purchase a new IHC system. >>> Currently we are working with Dako. >>> I would request your valuable feedback on :- >>> >>> 1- Bond max ( Leica) >>> 2- Bench Mark Ultra (Roche Diagnostic) >>> >>> Workload is 15,000-20,000 slides/year. >>> >>> Many thanks in advance for your help. >>> >>> Kind regards >>> W.A Hassan >>> >>> >>> >>> >>> ---------- Forwarded message ---------- >>> From: Tina Van Meter >>> To: warda hassan >>> Cc: Histonet at lists.utsouthwestern.edu >>> Bcc: >>> Date: Wed, 28 Oct 2020 23:08:07 -0400 >>> Subject: Re: [Histonet] Selection of IHC system >>> Bond Max >>> >>> On Wed, Oct 28, 2020, 2:53 PM warda hassan via Histonet < >>> histonet at lists.utsouthwestern.edu> wrote: >>> >>> > Dear Histonet >>> > >>> > Our lab is looking to purchase a new IHC system. >>> > Currently we are working with Dako. >>> > I would request your valuable feedback on :- >>> > >>> > 1- Bond max ( Leica) >>> > 2- Bench Mark Ultra (Roche Diagnostic) >>> > >>> > Workload is 15,000-20,000 slides/year. >>> > >>> > Many thanks in advance for your help. >>> > >>> > Kind regards >>> > W.A Hassan >>> > _______________________________________________ >>> > Histonet mailing list >>> > Histonet at lists.utsouthwestern.edu >>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> > >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> From mbireir at yahoo.com Fri Oct 30 23:07:21 2020 From: mbireir at yahoo.com (MANAHIL EL BIREIR) Date: Sat, 31 Oct 2020 17:07:21 +1300 Subject: [Histonet] DIF in paraffin section References: <3DC46008-A29B-4F1A-BE83-95F2C0117FC9.ref@yahoo.com> Message-ID: <3DC46008-A29B-4F1A-BE83-95F2C0117FC9@yahoo.com> Hi Histonet: Happy Halloween ? Would like to know if any one shift from DIF in fresh frozen section to embedded paraffin section!? And which IHC auto machine are you using ? Cheers, Have a nice weekend Manahil Sent from my iPhone