From histo at pathlab.us Fri May 1 08:13:32 2020 From: histo at pathlab.us (Histology) Date: Fri, 1 May 2020 13:13:32 +0000 Subject: [Histonet] Leica ST5020 vs Spectra Message-ID: Hi all, We have a Leica ST5010 that we have had forever and love. We have gotten a lot busier and need to eliminate the backup we currently get every day waiting to load the racks. I think we need an extra oven or 2. So we are looking into updating and will probably go with the ST5020 or the Spectra. Can anyone share their thoughts about these 2 stainers? We will probably only use this for one program (H&Es, no specials). Particularly, I am interested in the reagent usage. Do you have to use certain reagents and dump or rotate when the machine says? Any thoughts are greatly appreciated. Thanks, Mehndi Helgren From k.castillo at azdermgroup.com Fri May 1 10:00:49 2020 From: k.castillo at azdermgroup.com (Kristy Castillo) Date: Fri, 1 May 2020 08:00:49 -0700 Subject: [Histonet] IHC DAB Message-ID: Hi Histonetters, We are starting our IHC (fun times), we are having trouble with the DAB lighting up. Processed for 5 thru 10 minutes and still nothing. Our permanent red is working just fine. Any ideas. Thank you! Kristy From Daniel.Pesino at TrinityHealthOfNE.org Fri May 1 10:07:54 2020 From: Daniel.Pesino at TrinityHealthOfNE.org (Daniel Pesino) Date: Fri, 1 May 2020 15:07:54 +0000 Subject: [Histonet] [External] IHC DAB In-Reply-To: References: Message-ID: Hi Kristy, What platform and detection kit are you using? Would you mind sharing your DAB protocol? Thank you, Daniel Pesino, HTL(ASCP)CM QIHC CM Senior Histotechnologist Trinity Health Of New England Daniel.Pesino at TrinityHealthOfNE.org W? 860-714-4675 114 Woodland Street Hartford, CT 06105 TrinityHealthOfNE.org | Facebook | Twitter | Instagram -----Original Message----- From: Kristy Castillo via Histonet Sent: Friday, May 1, 2020 11:01 AM To: histonet at lists.utsouthwestern.edu Subject: [External] [Histonet] IHC DAB Warning: This email originated from the Internet! DO NOT CLICK links if the sender is unknown, and NEVER provide your password. Hi Histonetters, We are starting our IHC (fun times), we are having trouble with the DAB lighting up. Processed for 5 thru 10 minutes and still nothing. Our permanent red is working just fine. Any ideas. Thank you! Kristy _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From portera at msu.edu Fri May 1 10:16:36 2020 From: portera at msu.edu (Porter, Amy) Date: Fri, 1 May 2020 15:16:36 +0000 Subject: [Histonet] IHC DAB In-Reply-To: References: Message-ID: Hydrogen Peroxide gone bad / or not being added??? ________________________________ From: Kristy Castillo via Histonet Sent: Friday, May 1, 2020 11:00 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC DAB Hi Histonetters, We are starting our IHC (fun times), we are having trouble with the DAB lighting up. Processed for 5 thru 10 minutes and still nothing. Our permanent red is working just fine. Any ideas. Thank you! Kristy _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nqBcFt8qtbMg7kzneedVpPwolLzEfsp6AKUFOKtTOI2wNvOmwykUTuORZ3qW4g$ From vtolley25 at gmail.com Fri May 1 10:53:38 2020 From: vtolley25 at gmail.com (Val Tolley) Date: Fri, 1 May 2020 08:53:38 -0700 Subject: [Histonet] IHC DAB In-Reply-To: References: Message-ID: It?s difficult to know without your full protocol, but you can easily test your DAB. Take a paper towel and add a drop of your secondary/HRP. Add a drop of DAB to that. If it doesn?t immediately turn brown, your hydrogen peroxide has likely degraded into water. Make a fresh batch of DAB and try again. > On May 1, 2020, at 8:28 AM, Kristy Castillo via Histonet wrote: > > ?Hi Histonetters, > > We are starting our IHC (fun times), we are having trouble with the DAB > lighting up. Processed for 5 thru 10 minutes and still nothing. Our > permanent red is working just fine. Any ideas. > > Thank you! > > Kristy > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From k.castillo at azdermgroup.com Fri May 1 10:55:09 2020 From: k.castillo at azdermgroup.com (Kristy Castillo) Date: Fri, 1 May 2020 08:55:09 -0700 Subject: [Histonet] IHC DAB In-Reply-To: References: Message-ID: Thanks for the tip! On Fri, May 1, 2020 at 8:53 AM Val Tolley wrote: > It?s difficult to know without your full protocol, but you can easily test > your DAB. > > Take a paper towel and add a drop of your secondary/HRP. Add a drop of > DAB to that. If it doesn?t immediately turn brown, your hydrogen peroxide > has likely degraded into water. Make a fresh batch of DAB and try again. > > > > > On May 1, 2020, at 8:28 AM, Kristy Castillo via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > > ?Hi Histonetters, > > > > We are starting our IHC (fun times), we are having trouble with the DAB > > lighting up. Processed for 5 thru 10 minutes and still nothing. Our > > permanent red is working just fine. Any ideas. > > > > Thank you! > > > > Kristy > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From paula at excaliburpathology.com Fri May 1 11:01:17 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Fri, 1 May 2020 16:01:17 +0000 (UTC) Subject: [Histonet] IHC DAB In-Reply-To: References: Message-ID: <1506150376.300290.1588348877862@mail.yahoo.com> Since you are newbies, I am just putting IHC 101 out there.? Permanent red is for alkaline phosphatase detection and DAB is for HRP. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Friday, May 1, 2020, 10:29:00 AM CDT, Kristy Castillo via Histonet wrote: Hi Histonetters, We are starting our IHC (fun times), we are having trouble with the DAB lighting up.? Processed for 5 thru 10 minutes and still nothing.? Our permanent red is working just fine.? Any ideas. Thank you! Kristy _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmyers1 at aol.com Fri May 1 13:49:58 2020 From: jmyers1 at aol.com (Joe Myers) Date: Fri, 1 May 2020 14:49:58 -0400 Subject: [Histonet] IHC DAB References: <7A404F50-6D6A-40FE-8A6C-1BDC8FD8BD16.ref@aol.com> Message-ID: <7A404F50-6D6A-40FE-8A6C-1BDC8FD8BD16@aol.com> Kristy: I?m thinking along the same lines as Paula; is it possible that your detection reagent contains only Alkaline Phosphatase (ALP) as there a reactive enzyme? If so, a peroxide/DAB solution simply won?t react with it. Can?t wait to see your protocol, with detailed descriptions of the antigen, pretreatment reagent and ?labeling? enzyme. Cheers, Joe Myers, M.S.,CT/QIHC(ASCP) ________________________________ From: Kristy Castillo via Histonet Sent: Friday, May 1, 2020 11:00 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC DAB Hi Histonetters, We are starting our IHC (fun times), we are having trouble with the DAB lighting up. Processed for 5 thru 10 minutes and still nothing. Our permanent red is working just fine. Any ideas. Thank you! Kristy From Ana.Maluenda at baker.edu.au Sat May 2 19:29:55 2020 From: Ana.Maluenda at baker.edu.au (Ana Maluenda) Date: Sun, 3 May 2020 00:29:55 +0000 Subject: [Histonet] IHC DAB In-Reply-To: <7A404F50-6D6A-40FE-8A6C-1BDC8FD8BD16@aol.com> References: <7A404F50-6D6A-40FE-8A6C-1BDC8FD8BD16.ref@aol.com>, <7A404F50-6D6A-40FE-8A6C-1BDC8FD8BD16@aol.com> Message-ID: <90dd182188b94c5297b7c1ae26cad742@baker.edu.au> Hi Kristy, As the others said, so many things that could be on the way...But making sure your system is based on a HRP enzyme is definitely a good start, as mentioned by Joe. If you can share details, I might be able to help too. Welcome to the IHC world! Cheers, Ana Ana Maluenda Research Assistant/Laboratory Manager Atherothrombosis and Vascular Biology Laboratory ________________________________ From: Joe Myers Sent: Saturday, 2 May 2020 4:49 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC DAB Kristy: I?m thinking along the same lines as Paula; is it possible that your detection reagent contains only Alkaline Phosphatase (ALP) as there a reactive enzyme? If so, a peroxide/DAB solution simply won?t react with it. Can?t wait to see your protocol, with detailed descriptions of the antigen, pretreatment reagent and ?labeling? enzyme. Cheers, Joe Myers, M.S.,CT/QIHC(ASCP) ________________________________ From: Kristy Castillo via Histonet Sent: Friday, May 1, 2020 11:00 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC DAB Hi Histonetters, We are starting our IHC (fun times), we are having trouble with the DAB lighting up. Processed for 5 thru 10 minutes and still nothing. Our permanent red is working just fine. Any ideas. Thank you! Kristy Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting privacy at baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg From jfray80 at hotmail.com Sat May 2 21:56:45 2020 From: jfray80 at hotmail.com (Microsoft.com team) Date: Sun, 3 May 2020 02:56:45 +0000 Subject: [Histonet] CJD Decontamination Message-ID: Has anyone had to dispose of there processor for CJD contamination. And the embedding center. And is there a procedure for decontamination of equipment. Thank you. From making at ufl.edu Sun May 3 09:14:24 2020 From: making at ufl.edu (King,Michael A) Date: Sun, 3 May 2020 14:14:24 +0000 Subject: [Histonet] Histonet Digest, Vol 198, Issue 1 In-Reply-To: References: Message-ID: <1588515264240.15292@ufl.edu> Make sure sodium azide (antimicrobial preservative) is not used in any of your IHC reagents--it inhibits peroxidase. Mike ----------- Message: 3 Date: Fri, 1 May 2020 08:00:49 -0700 From: Kristy Castillo To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC DAB Message-ID: Content-Type: text/plain; charset="UTF-8" Hi Histonetters, We are starting our IHC (fun times), we are having trouble with the DAB lighting up. Processed for 5 thru 10 minutes and still nothing. Our permanent red is working just fine. Any ideas. Thank you! Kristy From jsagasser at gandhigi.com Mon May 4 09:40:47 2020 From: jsagasser at gandhigi.com (Jacque Sagasser) Date: Mon, 4 May 2020 14:40:47 +0000 Subject: [Histonet] p53 Message-ID: Hello, Our lab has been using this down time to validate a few antibodies. So far we have validated Ki67, AE 1/3, CK20, and CDX-2. I am in the process of validating the P53 (DO-7) antibody, but am having trouble producing positive expression. I am using the same colon adenocarcinoma tissue I used to validate all of the antibodies that I previously mentioned. We are using the Ventana Benchmark XT along with the OptiView DAB. The package insert says to use colon adenocarcinoma for a control tissue, and the iView DAB. In the past, I have worked at a facility that ran it using the UltraView DAB so I don't think using the OptiView DAB is the issue. My Roche representative said he has heard of customers having to use a different control for the P53. Do you have any suggestions for controls or tweaking the protocol for this antibody given the parameters we are using? Any advice would be appreciated. ?Jacque R. Sagasser, HT (ASCP)cm Gandhi GI Pathology, LLC Kettering OH From john.garratt at ciqc.ca Mon May 4 10:38:31 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Mon, 04 May 2020 15:38:31 +0000 Subject: [Histonet] p53 In-Reply-To: References: Message-ID: http://cpqa.ca/assessments/Run%2095%20p53%20Summary%20(final).pdf The above is an EQA report from CPQA /CIQC for p53 which gives specific protocols. In addition, if you go to the end of the report, there are the reporting guidelines for p53. EQA reports are also available for other antibodies when you go to our web site. John www.cpqa.ca ??????? Original Message ??????? On Monday, May 4, 2020 7:40 AM, Jacque Sagasser via Histonet wrote: > Hello, > > Our lab has been using this down time to validate a few antibodies. So far we have validated Ki67, AE 1/3, CK20, and CDX-2. I am in the process of validating the P53 (DO-7) antibody, but am having trouble producing positive expression. I am using the same colon adenocarcinoma tissue I used to validate all of the antibodies that I previously mentioned. We are using the Ventana Benchmark XT along with the OptiView DAB. The package insert says to use colon adenocarcinoma for a control tissue, and the iView DAB. In the past, I have worked at a facility that ran it using the UltraView DAB so I don't think using the OptiView DAB is the issue. My Roche representative said he has heard of customers having to use a different control for the P53. Do you have any suggestions for controls or tweaking the protocol for this antibody given the parameters we are using? Any advice would be appreciated. > > Jacque R. Sagasser, HT (ASCP)cm > Gandhi GI Pathology, LLC > Kettering OH > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDavis at che-east.org Mon May 4 13:25:40 2020 From: CDavis at che-east.org (Cassie P. Davis) Date: Mon, 4 May 2020 18:25:40 +0000 Subject: [Histonet] IHC control blocks, DAB kit Message-ID: Kristy, this might be of some help too: https://www.fixationonhistology.com/post/control-tissue-faqs [https://static.wixstatic.com/media/128854_2fda1d8f8d5c48cdad7701b3e866d885~mv2.jpg/v1/fit/w_1000,h_728,al_c,q_80/file.png] Control Tissue FAQs - fixationonhistology.com Should control slides be refrigerated? Some controls such as placenta, p53, and p57, for example, may lose antigenicity (the antigen?s ability to bind with the products of the cell mediated response, like B-cells and T-cells), if they are left out. Others are fine being left on the shelf. Whether or not you need to refrigerate them also depends on how far in advance they have been cut. www.fixationonhistology.com Cassie Davis Histology Department Anatomical Pathology Laboratory 302-575-8095 Email: CDavis at che-east.org W 302-575-8095 701 North Clayton St. Wilmington, DE 19805 trinity-health.org | Facebook | Twitter | LinkedIn [cid:image003.png at 01D29F21.CF07D5A0] Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From tissuearray at hotmail.com Tue May 5 20:48:22 2020 From: tissuearray at hotmail.com (Thom) Date: Wed, 6 May 2020 01:48:22 +0000 Subject: [Histonet] Tissue Microarray Consultant Questions Message-ID: Hi, I have a question for everyone. I have been creating tissue microarrays for 20 years. I have constructed hundreds of TMAs of all sizes and tissue types. I also have several articles on TMA construction published in journals (listed below). I am wondering if there is a need for my expertise in either training technicians or creating TMAs for laboratory needs. I can bring my own TMA instrument so no need to purchase your own equipment or I can use your TMA instrument. I have used different instruments over the years. For example, I can come to your lab and construct the TMA blocks using your materials, cut as many slides as needed also teach your techs my techniques and processes during my visit. Just wondering if there is a need for this type of consulting service? Thanks for your time. Thom Thom Jensen (HT ASCP) TMA Technician jensenthom at gmail.com TMA Publications The Tissue Microarray - A Technical Guid for Histologist The Journal of Histotechnology, / Vol 24, No. 4 / Dec. 2001 Thom Jensen, M. Elizabeth H. Hammond Tissue Microarray; Advanced Techniques The Journal of Histotechnology, / Vol 26, No. 2 / June 2003 Thom Jensen (HT ASCP) Tissue microarray advanced techniques for sampling donor blocks with limited tissues ? introduction to ?the radical-TMA? Journal of Histotechnology / Vol. 37, No. 1 / 2014 Thom Jensen, Melissa Cessna, Dylan Miller, Janet Hansen From k.castillo at azdermgroup.com Wed May 6 07:43:22 2020 From: k.castillo at azdermgroup.com (Kristy Castillo) Date: Wed, 6 May 2020 05:43:22 -0700 Subject: [Histonet] IHC validation Message-ID: Would like to know when validating for IHC for a dermatology lab and just for CLIA (no CAP), do you just need to show a shave, punch and excision lighting up? Thanks! From jmyers1 at aol.com Wed May 6 15:08:36 2020 From: jmyers1 at aol.com (Joe Myers) Date: Wed, 6 May 2020 16:08:36 -0400 Subject: [Histonet] IHC validation References: Message-ID: Kristy: When validating an IHC procedure, regulatory guidelines like CLIA are not concerned so much with the types of specimens upon which the procedure will be applied as they are with how appropriately a given procedure detects different levels of protein expression, which, in turn, usually correlates to the ?degree of disease?. In practical terms, this means that your lab?s efforts to validate should involve the staining of different tissues of the same type (i.e. skin), where the expression level ranges from ?low? to ?high?. There are certainly a great deal more issues involved in procedure validation, but this is my attempt to answer your initial question. Joe Myers, M.S., CT/QIHC(ASCP) *************************** Message: 2 Date: Wed, 6 May 2020 05:43:22 -0700 From: Kristy Castillo To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC validation Would like to know when validating for IHC for a dermatology lab and just for CLIA (no CAP), do you just need to show a shave, punch and excision lighting up? Thanks! From k.castillo at azdermgroup.com Fri May 8 07:41:23 2020 From: k.castillo at azdermgroup.com (Kristy Castillo) Date: Fri, 8 May 2020 05:41:23 -0700 Subject: [Histonet] Regulation for grossing derms Message-ID: My question is: What would be the regulation (CLIA) to gross dermatology specimens? Are they not considered high complexity? Also, don't you have to have a HT to do this? Someone is telling me they can gross as long as they have associates. Is that correct? Sorry, I am a little confused with this. Thankful for all who answer this question. Kristy AZDermgroup, Histology Lab From Mark.Pawlowski at jefferson.edu Fri May 8 12:17:14 2020 From: Mark.Pawlowski at jefferson.edu (Mark Pawlowski) Date: Fri, 8 May 2020 17:17:14 +0000 Subject: [Histonet] Grossing regulations In-Reply-To: References: Message-ID: <3EA87019-5EAF-498B-B913-159AF2695197@jefferson.edu> Hello Kristy, The regulation say that you must have at least an Associates degree with a certain amount of credits in the sciences. And yes, it is considered high complexity. Sorry I don?t remember the amount of credits. Mark > On May 8, 2020, at 1:00 PM, "histonet-request at lists.utsouthwestern.edu" wrote: > > ?WARNING: External Email - This email originated outside of Jefferson. > DO NOT CLICK links or attachments unless you recognize the sender and are expecting the email. > > > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cmark.pawlowski%40jefferson.edu%7C0b3de7ac2a3148c4bd3608d7f3714da2%7C55a89906c710436bbc444c590cb67c4a%7C0%7C0%7C637245540350393499&sdata=xL1wL5DeZ0wRHslbEnyPHyaXNzflfVriKNB%2BXTj%2FJ6g%3D&reserved=0 > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Regulation for grossing derms (Kristy Castillo) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 8 May 2020 05:41:23 -0700 > From: Kristy Castillo > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Regulation for grossing derms > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > My question is: What would be the regulation (CLIA) to gross dermatology > specimens? Are they not considered high complexity? Also, don't you have > to have a HT to do this? Someone is telling me they can gross as long as > they have associates. Is that correct? Sorry, I am a little confused with > this. Thankful for all who answer this question. > > Kristy > AZDermgroup, Histology Lab > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cmark.pawlowski%40jefferson.edu%7C0b3de7ac2a3148c4bd3608d7f3714da2%7C55a89906c710436bbc444c590cb67c4a%7C0%7C0%7C637245540350393499&sdata=xL1wL5DeZ0wRHslbEnyPHyaXNzflfVriKNB%2BXTj%2FJ6g%3D&reserved=0 > > ------------------------------ > > End of Histonet Digest, Vol 198, Issue 8 > **************************************** The information contained in this transmission contains privileged and confidential information. It is intended only for the use of the person named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. CAUTION: Intended recipients should NOT use email communication for emergent or urgent health care matters. From tbraud at holyredeemer.com Mon May 11 06:57:07 2020 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 11 May 2020 11:57:07 +0000 Subject: [Histonet] grossing regulations Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C4BD8E68@HRHEX02-HOS.holyredeemer.local> Actually, there is another route for some of us older techs. You can be grandfathered in without a degree if you have a certain prerequisite number of hours in specific science courses and prior documented training in grossing. Of course, I'm so old, I don't remember the details. LOL Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Saturday, May 09, 2020 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 198, Issue 9 CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links or opening attachments. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Grossing regulations (Mark Pawlowski) ---------------------------------------------------------------------- Message: 1 Date: Fri, 8 May 2020 17:17:14 +0000 From: Mark Pawlowski To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Grossing regulations Message-ID: <3EA87019-5EAF-498B-B913-159AF2695197 at jefferson.edu> Content-Type: text/plain; charset="utf-8" Hello Kristy, The regulation say that you must have at least an Associates degree with a certain amount of credits in the sciences. And yes, it is considered high complexity. Sorry I don?t remember the amount of credits. Mark > On May 8, 2020, at 1:00 PM, "histonet-request at lists.utsouthwestern.edu" wrote: > > ?WARNING: External Email - This email originated outside of Jefferson. > DO NOT CLICK links or attachments unless you recognize the sender and are expecting the email. > > > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cmark.pawlowski%40jefferson.edu%7C0b3de7ac2a3148c4bd3608d7f3714da2%7C55a89906c710436bbc444c590cb67c4a%7C0%7C0%7C637245540350393499&sdata=xL1wL5DeZ0wRHslbEnyPHyaXNzflfVriKNB%2BXTj%2FJ6g%3D&reserved=0 > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Regulation for grossing derms (Kristy Castillo) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 8 May 2020 05:41:23 -0700 > From: Kristy Castillo > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Regulation for grossing derms > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > My question is: What would be the regulation (CLIA) to gross dermatology > specimens? Are they not considered high complexity? Also, don't you have > to have a HT to do this? Someone is telling me they can gross as long as > they have associates. Is that correct? Sorry, I am a little confused with > this. Thankful for all who answer this question. > > Kristy > AZDermgroup, Histology Lab > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cmark.pawlowski%40jefferson.edu%7C0b3de7ac2a3148c4bd3608d7f3714da2%7C55a89906c710436bbc444c590cb67c4a%7C0%7C0%7C637245540350393499&sdata=xL1wL5DeZ0wRHslbEnyPHyaXNzflfVriKNB%2BXTj%2FJ6g%3D&reserved=0 > > ------------------------------ > > End of Histonet Digest, Vol 198, Issue 8 > **************************************** The information contained in this transmission contains privileged and confidential information. It is intended only for the use of the person named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. CAUTION: Intended recipients should NOT use email communication for emergent or urgent health care matters. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 198, Issue 9 **************************************** From Johanna.Haddock at propath.com Mon May 11 07:55:39 2020 From: Johanna.Haddock at propath.com (Johanna Haddock) Date: Mon, 11 May 2020 12:55:39 +0000 Subject: [Histonet] grossing regulations In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F801C4BD8E68@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F801C4BD8E68@HRHEX02-HOS.holyredeemer.local> Message-ID: Hi, Grossing is considered by CLIA a high complexity test therefore the testing personnel must be in compliance with the requirements to perform the test, in this case grossing . It doesn't matter what kind of tissue. Reference CLIA (a)(6) Specimen processing. Interpretive Guidelines ?493.1242(a)(6) Specimen processing may include receiving the specimen, accessioning the specimen, preparing the specimen for in-house analysis, preparation to send to a reference laboratory, preparing slides, and inoculating primary culture media, etc. Specimen processing also includes: Parasitology: the fixation and concentration of specimens; Virology: the pretreatment of specimens with antibiotics, the manipulation of cell culture tubes and inoculation of the cell cultures prior to incubation; Mycobacteriology: performing digestion-decontamination and concentration procedures on clinical specimens; and Histopathology: specimen accession with or without fixation, embedding the paraffin block, cutting the paraffin block, mounting the embedded cut tissue to a slide, preparing the slide for staining, staining and cover slipping the slide, or any other slide preparation procedures that do not involve examination resulting in diagnostic interpretation. Note: for histopathology specimens, specimen processing does not constitute a CLIA test. Only gross examinations (including weighing, measuring, describing color, specific orientation for diagnostic interpretation, and other characteristics of the tissue, or performing other mechanical procedures including dissection, inking, and marking) require a CLIA certificate. Microscopic examinations of tissue with diagnostic interpretation and reporting is a Histopathology test and requires CLIA certification. Personnel: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under the CLIA regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a chemical or biological science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes the following: ? 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes six semester hours of chemistry, six semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination, AND ? Laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLS, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least three months of recorded laboratory training in each specialty in which the individual performs high complexity testing. Thanks, Johanna Haddock QA Manager -----Original Message----- From: Terri Braud via Histonet Sent: Monday, May 11, 2020 6:57 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] grossing regulations [EXTERNAL] Actually, there is another route for some of us older techs. You can be grandfathered in without a degree if you have a certain prerequisite number of hours in specific science courses and prior documented training in grossing. Of course, I'm so old, I don't remember the details. LOL Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Saturday, May 09, 2020 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 198, Issue 9 CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links or opening attachments. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Grossing regulations (Mark Pawlowski) ---------------------------------------------------------------------- Message: 1 Date: Fri, 8 May 2020 17:17:14 +0000 From: Mark Pawlowski To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Grossing regulations Message-ID: <3EA87019-5EAF-498B-B913-159AF2695197 at jefferson.edu> Content-Type: text/plain; charset="utf-8" Hello Kristy, The regulation say that you must have at least an Associates degree with a certain amount of credits in the sciences. And yes, it is considered high complexity. Sorry I don?t remember the amount of credits. Mark > On May 8, 2020, at 1:00 PM, "histonet-request at lists.utsouthwestern.edu" wrote: > > ?WARNING: External Email - This email originated outside of Jefferson. > DO NOT CLICK links or attachments unless you recognize the sender and are expecting the email. > > > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > > https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists > .utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7 > Cmark.pawlowski%40jefferson.edu%7C0b3de7ac2a3148c4bd3608d7f3714da2%7C5 > 5a89906c710436bbc444c590cb67c4a%7C0%7C0%7C637245540350393499&sdata > =xL1wL5DeZ0wRHslbEnyPHyaXNzflfVriKNB%2BXTj%2FJ6g%3D&reserved=0 > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Regulation for grossing derms (Kristy Castillo) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 8 May 2020 05:41:23 -0700 > From: Kristy Castillo > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Regulation for grossing derms > Message-ID: > > > Content-Type: text/plain; charset="UTF-8" > > My question is: What would be the regulation (CLIA) to gross > dermatology specimens? Are they not considered high complexity? Also, don't you have > to have a HT to do this? Someone is telling me they can gross as long as > they have associates. Is that correct? Sorry, I am a little confused > with this. Thankful for all who answer this question. > > Kristy > AZDermgroup, Histology Lab > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists > .utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7 > Cmark.pawlowski%40jefferson.edu%7C0b3de7ac2a3148c4bd3608d7f3714da2%7C5 > 5a89906c710436bbc444c590cb67c4a%7C0%7C0%7C637245540350393499&sdata > =xL1wL5DeZ0wRHslbEnyPHyaXNzflfVriKNB%2BXTj%2FJ6g%3D&reserved=0 > > ------------------------------ > > End of Histonet Digest, Vol 198, Issue 8 > **************************************** The information contained in this transmission contains privileged and confidential information. It is intended only for the use of the person named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. CAUTION: Intended recipients should NOT use email communication for emergent or urgent health care matters. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 198, Issue 9 **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From redrose297 at gmail.com Wed May 13 03:05:47 2020 From: redrose297 at gmail.com (warda hassan) Date: Wed, 13 May 2020 12:05:47 +0400 Subject: [Histonet] Spectra Staining kits Message-ID: Dear Histonets Firstly would like to thank to all who had helped me in sorting out my previous issues with Spectra System, extremely appreciated. As going through different protocols i noticed none are bond to use leica staining kits but with me we are bond to use their kits. Currently we are using H2 kits, the kits states 2000 slides with constant staining pattern. Unfortunately we had encountered the kit quality drops after 800 slides only, in addition now it has started to drop after a week only, the option . of agitation was tried but it gave us extremely pale results. If anyone has experienced similar issues using Leica H/E kits please do share your opinion or suggestions Many thanks to all in advanced Kind Regards Warda Hassan From akemiat3377 at gmail.com Wed May 13 06:07:42 2020 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Wed, 13 May 2020 05:07:42 -0600 Subject: [Histonet] Bx Processing run Message-ID: <553BB819-7BE9-4D77-8EAB-AD511A6F4FB4@gmail.com> Good morning Histopeeps: I am reaching out to you this morning to ask for your processing protocols for your biopsies and routines. Our department has two 25 year old VIP Tissue processors. Prior to my arrival, the department was only using one processor at a time and running the biopsies and routine tissues together. They were also only rotating solutions once a week even though there were close to 300 cassettes a day. With that being said, we are now using both tissue processors and separating biopsies from routines and we rotate all solutions daily. The specimens are put on the machines at 5:00 PM. This is not an option. Our biopsies finish at 5:00 AM and the routines finish at 6:30 AM. I would like to have our biopsies finish by 3:00 AM and the routines finish by 5:00 AM. Naturally, I do not want to compromise the quality of our tissue samples. I would appreciate you sharing your processing protocols with me which would include solutions and time in each station. Thank you in advance for your information. Akemi Allison, BS, HT/HTL (ASCP) Histology Supervisor UMC El Paso, TX From craigak12 at gmail.com Wed May 13 16:24:23 2020 From: craigak12 at gmail.com (J B) Date: Wed, 13 May 2020 14:24:23 -0700 Subject: [Histonet] LIS System Recommendations: Message-ID: Hi there, I am looking for a new LIS system. Can anyone recommend a good product for a AP lab? We handle all specimen types. Thank you, Julie From greg.dobbin at gmail.com Thu May 14 06:30:24 2020 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Thu, 14 May 2020 08:30:24 -0300 Subject: [Histonet] Spectra Staining Kits Message-ID: Hi Wanda, If you have just purchased the Spectra stainer recently (ie still under the first year warranty), demand that Leica send their Application Specialist back on site. if they promised 2000 slides with no drop in quality over that period (I believe it is 2000 slides or 7 days, whichever come first)...then hold them to it! Make them work for you. You paid a lot of money for that system. You should not need to be on here looking for help. I have a Spectra and if you want to chat with me about our experience feel free to call me. I'll send you my number in a separate email. All the best, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From tbraud at holyredeemer.com Thu May 14 12:25:43 2020 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 14 May 2020 17:25:43 +0000 Subject: [Histonet] LIS Systems Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C4BD9795@HRHEX02-HOS.holyredeemer.local> I've had great personal experiences with Sunquest CoPath Plus. It is very flexible for customization and at 3 differing institutions, has been extremely reliable. This does, however, come at a rather expensive cost. I think that in choosing a system, one needs to take into account connectivity to instrumentation, EMRs, billing, and if the system has to interface with another IS, or is stand alone. I'm sure there are some other good systems out there. Best of luck, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, May 14, 2020 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 198, Issue 12 CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links or opening attachments. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. LIS System Recommendations: (J B) 2. Re: Spectra Staining Kits (Greg Dobbin) ---------------------------------------------------------------------- Message: 1 Date: Wed, 13 May 2020 14:24:23 -0700 From: J B To: histonet at lists.utsouthwestern.edu Subject: [Histonet] LIS System Recommendations: Message-ID: Content-Type: text/plain; charset="UTF-8" Hi there, I am looking for a new LIS system. Can anyone recommend a good product for a AP lab? We handle all specimen types. Thank you, Julie ------------------------------ Message: 2 Date: Thu, 14 May 2020 08:30:24 -0300 From: Greg Dobbin To: redrose297 at gmail.com Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Spectra Staining Kits Message-ID: Content-Type: text/plain; charset="UTF-8" Hi Wanda, If you have just purchased the Spectra stainer recently (ie still under the first year warranty), demand that Leica send their Application Specialist back on site. if they promised 2000 slides with no drop in quality over that period (I believe it is 2000 slides or 7 days, whichever come first)...then hold them to it! Make them work for you. You paid a lot of money for that system. You should not need to be on here looking for help. I have a Spectra and if you want to chat with me about our experience feel free to call me. I'll send you my number in a separate email. All the best, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 198, Issue 12 ***************************************** From plucas at biopath.org Thu May 14 13:28:56 2020 From: plucas at biopath.org (Paula) Date: Thu, 14 May 2020 11:28:56 -0700 Subject: [Histonet] UV Light in Fume Hood Message-ID: <005301d62a1d$882dcf10$98896d30$@biopath.org> Hello everyone.. I hope all is well. My admin wants me to look into possibly adding a UV light inside our fume hood, which currently does not have one. I'm thinking maybe it's not as effective to kill viruses as we think it would be. If anyone can share your thoughts about it, or if anyone has bought one and has any insights to share, that would be greatly appreciated. Have a great day and take care, Paula From paula at excaliburpathology.com Thu May 14 14:12:16 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Thu, 14 May 2020 19:12:16 +0000 (UTC) Subject: [Histonet] UV Light in Fume Hood In-Reply-To: <005301d62a1d$882dcf10$98896d30$@biopath.org> References: <005301d62a1d$882dcf10$98896d30$@biopath.org> Message-ID: <1803443367.238675.1589483536292@mail.yahoo.com> It will need to be UVC or UVD. UV light works by scrabbling the DNA in bacteria, viruses, and mold. But I will also scrabble yours too. Hospitals, airports, and other businesses are starting to use UVD robots to disinfect patient rooms and large areas with no people present at the time. UV light can be an added way to disinfect your hood, but get one with a timer and don't turn it on until you are ready to leave for the day and no housekeeping personnel will be in the lab during its use. Practically all of my work is shipped in and I have a UVC light in a closet and zap packages so I can open them sooner. Some household air cleaners also have UV light incorporated into their system. OION Technologies is one brand. No commercial stake, I am just an end user of it at home.? Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, May 14, 2020, 01:33:36 PM CDT, Paula via Histonet wrote: Hello everyone.. I hope all is well. My admin wants me to look into possibly adding a UV light inside our fume hood, which currently does not have one.? I'm thinking maybe it's not as effective to kill viruses as we think it would be. If anyone can share your thoughts about it, or if anyone has bought one and has any insights to share, that would be greatly appreciated. Have a great day and take care, Paula _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles at uwhealth.org Thu May 14 14:20:43 2020 From: CIngles at uwhealth.org (Ingles Claire) Date: Thu, 14 May 2020 19:20:43 +0000 Subject: [Histonet] UV Light in Fume Hood In-Reply-To: <005301d62a1d$882dcf10$98896d30$@biopath.org> References: <005301d62a1d$882dcf10$98896d30$@biopath.org> Message-ID: UV-C wavelength is the best for the virus killing. Claire ________________________________ From: Paula via Histonet Sent: Thursday, May 14, 2020 1:28 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] UV Light in Fume Hood WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Hello everyone.. I hope all is well. My admin wants me to look into possibly adding a UV light inside our fume hood, which currently does not have one. I'm thinking maybe it's not as effective to kill viruses as we think it would be. If anyone can share your thoughts about it, or if anyone has bought one and has any insights to share, that would be greatly appreciated. Have a great day and take care, Paula _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at dpspa.com Fri May 15 14:13:10 2020 From: criley at dpspa.com (Charles Riley) Date: Fri, 15 May 2020 19:13:10 +0000 Subject: [Histonet] Ventana question Message-ID: Does anyone know if Ventana offers a FFPE PCR testing platform From rmccormick10 at yahoo.com Fri May 15 15:42:38 2020 From: rmccormick10 at yahoo.com (Yahoo) Date: Fri, 15 May 2020 15:42:38 -0500 Subject: [Histonet] Brain and spinal cord References: Message-ID: Hi All! I?m looking for some suggestions please on fixation for brain tissue and spinal cord submissions from necropsies. We are currently using 10% NBF and ask our pathologists to leave the samples overnight (but that doesn?t always happen!!). Does anyone use alcohol-based fixatives? And if so, how long? Does it affect IHC or any other staining? Do you still process with other routine biopsies (14 hour program)? Thanks! From maxim_71 at mail.ru Mon May 18 01:30:54 2020 From: maxim_71 at mail.ru (=?UTF-8?B?0J/QtdGI0LrQvtCyINCc0LDQutGB0LjQvA==?=) Date: Mon, 18 May 2020 09:30:54 +0300 Subject: [Histonet] =?utf-8?q?Article_form_old_journal?= Message-ID: <1589783454.522386922@f345.i.mail.ru> Dear histonetters! Can anyone share with me a scan of?article by Hinshaw, J. R. and Pierce, H. E. (Surg Gyn Obstet, 103:726-730, 1956) please? This article is not available in internet. Sincerely, Maxim Peshkov Russia, Taganrog. ? ? From jkiernan at uwo.ca Mon May 18 10:02:50 2020 From: jkiernan at uwo.ca (John Kiernan) Date: Mon, 18 May 2020 15:02:50 +0000 Subject: [Histonet] Brain and spinal cord In-Reply-To: References: , Message-ID: 10% neutral buffered formaldehyde is good, but a whole human brain needs to be immersed for at least 2 weeks, suspended by a string under the basilar artery to prevent squashing against the bottom of the container. The soft tissue has to be properly hardened before the brain can be sliced and small pieces selected for embedding and sectioning. Fixatives other than formaldehyde can be OK for brains of small animals used in research, but for human neuropathology you need formaldehyde to get the expected results with regularly used stains and with immunohistochemistry. See Adams & Murray 1982 Atlas of post-mortem techniques in neuropathology. ISBN 9780521105682. Secondhand copies cost about US $30. Every lab should have plenty of books. They cost a lot less than making mistakes. John Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada = = = ________________________________ From: Yahoo via Histonet Sent: 15 May 2020 15:42 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Brain and spinal cord Hi All! I?m looking for some suggestions please on fixation for brain tissue and spinal cord submissions from necropsies. We are currently using 10% NBF and ask our pathologists to leave the samples overnight (but that doesn?t always happen!!). Does anyone use alcohol-based fixatives? And if so, how long? Does it affect IHC or any other staining? Do you still process with other routine biopsies (14 hour program)? Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy.Stedman at buschgardens.com Mon May 18 10:46:02 2020 From: Nancy.Stedman at buschgardens.com (Stedman, Nancy) Date: Mon, 18 May 2020 15:46:02 +0000 Subject: [Histonet] [EXTERNAL] Brain and spinal cord In-Reply-To: References: Message-ID: Hi ? I use Excell Plus (alcohol based) for small brains (dog size and smaller). I book them after 24 hours and then let them sit another 24 hours or more depending on the size, and changing the Excell. It?s definitely not good as formalin, but my cases are veterinary necropsies and usually already have some autolysis already when they are submitted. IHC seems to work just fine. Have not tried ISH or nucleic acid extraction but the company that makes Excell Plus says it is suitable for these applications. I have tried other alcohol based fixatives for small brains and none have been as good as Excell Plus. -Nancy Stedman -----Original Message----- From: Yahoo via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, May 15, 2020 4:43 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] Brain and spinal cord Hi All! I?m looking for some suggestions please on fixation for brain tissue and spinal cord submissions from necropsies. We are currently using 10% NBF and ask our pathologists to leave the samples overnight (but that doesn?t always happen!!). Does anyone use alcohol-based fixatives? And if so, how long? Does it affect IHC or any other staining? Do you still process with other routine biopsies (14 hour program)? Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!O5V8a9i_ipf_jKx3!sMfBb9Xlczrc1L4sG9pQM5VLmHM7-E16GgDofTy-UzBz-G7msgznl-x3zjNPzwCWAWPVdfs$ From relia1 at earthlink.net Wed May 20 10:20:07 2020 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 20 May 2020 11:20:07 -0400 Subject: [Histonet] Great Advice from "Ask A Manager" - If you are furloughed how long should you wait to look for a new job? #ilovemyhistopeeps Message-ID: <009a01d62eba$2519f060$6f4dd120$@earthlink.net> Hi Histopeeps, I hope you and your loved ones are looking forward to a safe and healthy Memorial Day Weekend! In many states elective surgeries have resumed with most others soon to follow suit and I think it is safe to say that where elective surgeries go Histology follows! Histotechs are starting to be called back to work!!! Here is a link to a great article from "Ask A Manager" If you are furloughed how long should you wait to look for a new job? https://www.askamanager.org/2020/05/if-youre-furloughed-how-long-should-you- wait-to-look-for-a-new-job.html What's happening on your end? Have you been called back to work - full time or part time? Unemployed? Laid off? Furloughed? Are you a traveler that wants to get back to permanent work? Are you a histotech that wants to change the type of histology you do? Do you need a resume tune up? Do you need some career advice? I am here if you need me and would love to know what is happening on your end. If you have a minute shoot me back an email and let me know what's up and I promise to share with you what I am seeing in other places. What I still believe and glean from this and bear in mind this only an opinion on my part based on what I have heard anecdotally. It seems that the best case scenario at this point is there was a shortage of histotechs before the pandemic and there will be just as great a need after the pandemic in other words we have just been pressing pause. Just Remember. Things Will Get Better. This Will End. & We Will Return To Our Normal Lives. In the meantime. Is there anything I can do for you? Are you feeling lonely or isolated and just need to hear a friendly voice? Call Me, Text Me, Email Me, Or Contact Me through Social Media. We are all in this together. Looks like we are starting to see the light at the end of the tunnel. Have a Great Memorial Day Weekend! Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From richard.wild at sfr.fr Wed May 27 07:20:09 2020 From: richard.wild at sfr.fr (richard.wild at sfr.fr) Date: Wed, 27 May 2020 14:20:09 +0200 Subject: [Histonet] Shandon-Cryotome-Fse battery change ? Message-ID: Hi Is it possible to change battery that keeps usual data (language etc) on Shandon-Cryotome-Fse*?* And where is it ? Best Richard From paula at excaliburpathology.com Wed May 27 07:57:19 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Wed, 27 May 2020 12:57:19 +0000 (UTC) Subject: [Histonet] Shandon-Cryotome-Fse battery change ? In-Reply-To: References: Message-ID: <1497519085.259162.1590584239938@mail.yahoo.com> Yes, it is the same as the battery on a computer tower motherboard to keep the clock going. Turn off. Unplug. Remove panel. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Wednesday, May 27, 2020, 07:32:56 AM CDT, richard.wild--- via Histonet wrote: Hi Is it possible to change battery that keeps usual data (language etc) on Shandon-Cryotome-Fse*?* And where is it ? Best Richard _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed May 27 11:26:12 2020 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 27 May 2020 12:26:12 -0400 Subject: [Histonet] Lead Tech/Histology Superviser needed for a Growing lab in the Tampa Bay area! Message-ID: <000b01d63443$89cbb410$9d631c30$@earthlink.net> Hi Histopeeps, How are you? I hope you are having a great week! I have several exciting opportunities to share and if you aren?t interested maybe you know someone who might be! I have been engaged on searches by several clients located in the state of Florida. Here is the info: ? Tampa Bay ? Histology Supervisor ? Days ? Tampa Bay ? Lead Histology Tech ? Days The help I need from you Histopeeps is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them or pass their contact information to me? *remember if I place someone you refer to me you earn a referral bonus* Both of these positions are full time and permanent. And my clients are ready to interview and hire!! If you are interested in this position please contact me ASAP On my cell/text 407-353-5070 or toll free at 866-607-3542 Or via email at relia1 at earthlink.net If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won?t release it to anyone without your permission. Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From acoscetti at gmail.com Wed May 27 14:25:59 2020 From: acoscetti at gmail.com (Amanda Coscetti) Date: Wed, 27 May 2020 15:25:59 -0400 Subject: [Histonet] Grossing Message-ID: ?Hi everyone! Wondering if anyone knows of a place to look for standards on how long it takes to gross in surgical specimens.....if there is such a thing! Thanks for your help! Amanda Sent from my iPhone From c.tague at pathologyarts.com Wed May 27 18:53:18 2020 From: c.tague at pathologyarts.com (Curt Tague) Date: Wed, 27 May 2020 23:53:18 +0000 Subject: [Histonet] CLIA license expiration Message-ID: Looking for someone who might have experience with this already.... My CLIA and State license are going to expire soon and I've heard nothing from either the state or CLIA.... So I took the liberty of reaching out to them with no luck... I then called an inspector I know who suggested the main office might be willing or able to submit a letter indicating that things are still good, labs are permitted to continue until further notice as this COVID issue has put them further behind than they already usually are. So, my simple question is this, has anyone had to deal with this issue yet and if so, how did you get it resolved? Thanks! Curt From kdean70 at hotmail.com Thu May 28 09:17:02 2020 From: kdean70 at hotmail.com (Ken M) Date: Thu, 28 May 2020 14:17:02 +0000 Subject: [Histonet] H&E Staining Protocol Message-ID: Hello: Does anyone have a good manual or automatic H&E staining protocol that will work well on multiple Paraffin embedded tissues? Ken Kdean70 at hotmail.com From criley at dpspa.com Thu May 28 10:38:34 2020 From: criley at dpspa.com (Charles Riley) Date: Thu, 28 May 2020 15:38:34 +0000 Subject: [Histonet] CAP checklist help Message-ID: Does anyone have a policy for assessing professional competency (checklist item ANP. 10010)? I want to get ideas on how everyone performs this to verify we are meeting the best standards From Lisa.White3 at va.gov Thu May 28 12:14:00 2020 From: Lisa.White3 at va.gov (White, Lisa M.) Date: Thu, 28 May 2020 17:14:00 +0000 Subject: [Histonet] CMV Message-ID: Hello All, We are in process of optimization/validation of CMV. Does anyone have any controls to help us in the validation process? I may have some control tissue to trade for another stain. Any help appreciated. Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC 423-979-3567 423-979-3401 From Blanca.Lopez at UTSouthwestern.edu Thu May 28 12:56:01 2020 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Thu, 28 May 2020 17:56:01 +0000 Subject: [Histonet] Brain mouse protocol Message-ID: Hello All! Is anybody has a brain mouse protocol to share. This is for research biorepository lab. Also if you Can recommended fixative type to use and the time of fixation. I will appreciate Thank you. Blanca Lopez HT(ASCP) Histotechnologist UT Southwestern Medical Center Harold C Simmons Comprehensive Cancer Center Pathology Lab NB5-102 214-648-7598 blanca.lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From ctorrence at kmcpa.com Thu May 28 14:05:28 2020 From: ctorrence at kmcpa.com (Carol Torrence) Date: Thu, 28 May 2020 19:05:28 +0000 Subject: [Histonet] In search of Leica 2000 series part Message-ID: Good afternoon! I am in search of a high profile back plate for an older (probably should say OLD) Leica RM2025 blade holder. I brought the microtome up from the basement for a teaching microtome and I forgot that we had used low profile blades on it. All of our microtomes use high profile. If anyone has this part available, I would like to switch this one out or purchase a high profile plate. Email me to spare the group. LOL. Please disregard if this post is not appropriate. Thanks! And be well! Carol Torrence HT(ASCP) KMC DERMATOLOGY, Topeka, Kansas