From greg.dobbin at gmail.com Sun Mar 1 17:01:11 2020 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Sun, 1 Mar 2020 19:01:11 -0400 Subject: [Histonet] IHC troubleshooting Message-ID: Nancy, You used the word ?blot? in your reply to Charles, and while I can?t be certain that you did not mean the same...I would like say in the interest of clarity, that it is safer to carefully ?wick? the water away from under each section prior to baking. Blotting (like we might do after a Gram stain) would likely remove parts or all of the section. All the best, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From ASelf at tidelandshealth.org Tue Mar 3 09:31:59 2020 From: ASelf at tidelandshealth.org (Amy Self) Date: Tue, 3 Mar 2020 15:31:59 +0000 Subject: [Histonet] Incomplete cross sections of all tissue in blocks Message-ID: Good Morning HistoNetters, I am reaching out to the histonet in hopes to get some suggestions from you on how to handle incomplete cross-sections of tissue in blocks. We are a small lab so this has not been an issue in the past but now that we are growing and our staff has increased I am getting feed-back from pathologist that the sections of tissue are not complete. They are asking for too many deepers that possibly could be avoided if it was cut deep enough to begin with. I have been given some managerial type duties ? which I don?t like cause I know nothing about managing people and I need to approach this but I need to approach this issue correctly. Do you have the histotech compare his/her cut slides to the block to make sure that a complete cross-section is obtained and is this documented somehow? Any and all suggestions I need. Thanks in advance for your help and as always you all rock.. ? Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 (843) 520-8711 ASelf at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From john.garratt at ciqc.ca Tue Mar 3 11:01:55 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Tue, 03 Mar 2020 17:01:55 +0000 Subject: [Histonet] Incomplete cross sections of all tissue in blocks In-Reply-To: References: Message-ID: I suggest that each histotech is responsible for the blocks they cut and they cut the deepers on the their own blocks when they are requested. With feedback on the reason for the deeper from the pathologists they (the techs) will become more confident and learn how deep to cut. John On Tue, Mar 3, 2020 at 7:31 AM, Amy Self via Histonet wrote: > Good Morning HistoNetters, > > I am reaching out to the histonet in hopes to get some suggestions from you on how to handle incomplete cross-sections of tissue in blocks. We are a small lab so this has not been an issue in the past but now that we are growing and our staff has increased I am getting feed-back from pathologist that the sections of tissue are not complete. They are asking for too many deepers that possibly could be avoided if it was cut deep enough to begin with. I have been given some managerial type duties ? which I don?t like cause I know nothing about managing people and I need to approach this but I need to approach this issue correctly. Do you have the histotech compare his/her cut slides to the block to make sure that a complete cross-section is obtained and is this documented somehow? Any and all suggestions I need. > > Thanks in advance for your help and as always you all rock.. ? > > Amy Self > Histology Lab Senior Tech > Lab > Tidelands Georgetown Memorial Hospital > 606 Black River Road > Georgetown, SC 29440 > (843) 520-8711 > ASelf at tidelandshealth.org > Our mission: We help people live better lives through better health. > > NOTE: > The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aperl at caremount.com Tue Mar 3 12:36:12 2020 From: aperl at caremount.com (Perl, Alison) Date: Tue, 3 Mar 2020 18:36:12 +0000 Subject: [Histonet] [EXTERNAL] Re: Incomplete cross sections of all tissue in blocks In-Reply-To: References: Message-ID: <96e787bcf5684af6bb3b701beb77202a@MK-EXMB02.mkmg.com> Each of our techs is responsible for fully facing their blocks and getting a representative section. If the docs see something is amiss (epidermis missing, incomplete cross section, etc), they can order a Technical Recut rather than a routine Recut. Unfortunately, it sounds like you (or someone else of authority), has to give some feedback/coaching that the sections are insufficient, particularly if it's a recurring problem. Is it one tech that needs a 1-on-1 convo, or a meeting with the whole staff to address widespread issues? Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at caremount.com -----Original Message----- From: John Garratt via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, March 3, 2020 12:02 PM To: Amy Self; histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Incomplete cross sections of all tissue in blocks I suggest that each histotech is responsible for the blocks they cut and they cut the deepers on the their own blocks when they are requested. With feedback on the reason for the deeper from the pathologists they (the techs) will become more confident and learn how deep to cut. John On Tue, Mar 3, 2020 at 7:31 AM, Amy Self via Histonet wrote: > Good Morning HistoNetters, > > I am reaching out to the histonet in hopes to get some suggestions from you on how to handle incomplete cross-sections of tissue in blocks. We are a small lab so this has not been an issue in the past but now that we are growing and our staff has increased I am getting feed-back from pathologist that the sections of tissue are not complete. They are asking for too many deepers that possibly could be avoided if it was cut deep enough to begin with. I have been given some managerial type duties ? which I don?t like cause I know nothing about managing people and I need to approach this but I need to approach this issue correctly. Do you have the histotech compare his/her cut slides to the block to make sure that a complete cross-section is obtained and is this documented somehow? Any and all suggestions I need. > > Thanks in advance for your help and as always you all rock.. ? > > Amy Self > Histology Lab Senior Tech > Lab > Tidelands Georgetown Memorial Hospital > 606 Black River Road > Georgetown, SC 29440 > (843) 520-8711 > ASelf at tidelandshealth.org > Our mission: We help people live better lives through better health. > > NOTE: > The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Tue Mar 3 14:46:17 2020 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 3 Mar 2020 20:46:17 +0000 Subject: [Histonet] Incomplete sectioning Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B77C52@HRHEX02-HOS.holyredeemer.local> A quality check can be accomplished in 2 places. It can be done at cutting, but it should already be being done and it doesn't seem to be working. Ideally, the stained H&E should be checked against the block face as it is pulled from the coverslipper to be given to the pathologist. Then it can be handed immediately to the tech that made the original error to "do over" Also, a common excuse will be "it was embedded poorly". The answer to that is that it is the cutting tech's responsibility to hand back a poorly embedded block for it to be re-embedded if they feel that they can't get a representation section. Remember, it could also be poorly cut gross, too. Then you can nip it in the bud before the slide reaches the pathologist, and you can quickly identify who is turning out inferior sections and counsel them appropriately if needed. Hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Tuesday, March 03, 2020 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 196, Issue 2 CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links or opening attachments. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Incomplete cross sections of all tissue in blocks (Amy Self) 2. Re: Incomplete cross sections of all tissue in blocks (John Garratt) ---------------------------------------------------------------------- Message: 1 Date: Tue, 3 Mar 2020 15:31:59 +0000 From: Amy Self To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Incomplete cross sections of all tissue in blocks Message-ID: Content-Type: text/plain; charset="utf-8" Good Morning HistoNetters, I am reaching out to the histonet in hopes to get some suggestions from you on how to handle incomplete cross-sections of tissue in blocks. We are a small lab so this has not been an issue in the past but now that we are growing and our staff has increased I am getting feed-back from pathologist that the sections of tissue are not complete. They are asking for too many deepers that possibly could be avoided if it was cut deep enough to begin with. I have been given some managerial type duties ? which I don?t like cause I know nothing about managing people and I need to approach this but I need to approach this issue correctly. Do you have the histotech compare his/her cut slides to the block to make sure that a complete cross-section is obtained and is this documented somehow? Any and all suggestions I need. Thanks in advance for your help and as always you all rock.. ? Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 (843) 520-8711 ASelf at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ------------------------------ Message: 2 Date: Tue, 03 Mar 2020 17:01:55 +0000 From: John Garratt To: Amy Self , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Incomplete cross sections of all tissue in blocks Message-ID: Content-Type: text/plain; charset=UTF-8 I suggest that each histotech is responsible for the blocks they cut and they cut the deepers on the their own blocks when they are requested. With feedback on the reason for the deeper from the pathologists they (the techs) will become more confident and learn how deep to cut. John On Tue, Mar 3, 2020 at 7:31 AM, Amy Self via Histonet wrote: > Good Morning HistoNetters, > > I am reaching out to the histonet in hopes to get some suggestions from you on how to handle incomplete cross-sections of tissue in blocks. We are a small lab so this has not been an issue in the past but now that we are growing and our staff has increased I am getting feed-back from pathologist that the sections of tissue are not complete. They are asking for too many deepers that possibly could be avoided if it was cut deep enough to begin with. I have been given some managerial type duties ? which I don?t like cause I know nothing about managing people and I need to approach this but I need to approach this issue correctly. Do you have the histotech compare his/her cut slides to the block to make sure that a complete cross-section is obtained and is this documented somehow? Any and all suggestions I need. > > Thanks in advance for your help and as always you all rock.. ? > > Amy Self > Histology Lab Senior Tech > Lab > Tidelands Georgetown Memorial Hospital > 606 Black River Road > Georgetown, SC 29440 > (843) 520-8711 > ASelf at tidelandshealth.org > Our mission: We help people live better lives through better health. > > NOTE: > The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 196, Issue 2 **************************************** From Christopher.Hagon at act.gov.au Tue Mar 3 15:21:08 2020 From: Christopher.Hagon at act.gov.au (Hagon, Christopher (Health)) Date: Tue, 3 Mar 2020 21:21:08 +0000 Subject: [Histonet] Cutting benchmarks Message-ID: UNCLASSIFIED Hello Histonetters, Just wondering if anyone has had any benchmarks put in place in regards to blocks cut or slides produced per histotech/scientist per day? I realise that there are differing levels of complexity per block, we range from 1 slide per block to 21 one micron sections per renal block. Has any lab has WHS involved as far as Repetitive Strain Injuries are concerned? I'm not looking for any definitive answers that I'll hold anyone to, just if anyone has expectations of maximum throughput. We have a tracking system that we can accurately see how much work each person is doing. There seem to be limits on how many cases a pathologist can report in a day (again, varying levels of complexity) but what about for lab staff? Any thoughts greatly appreciated. Chris Hagon | Senior Scientist, Anatomical Pathology Phone: (02) 5124 2874 | Email: christopher.hagon at act.gov.au ACT Pathology | Canberra Health Services | ACT Government Canberra Hospital | Building 10 Level 1 | PO Box 11, Woden ACT 2606 | health.act.gov.au ----------------------------------------------------------------------- This email, and any attachments, may be confidential and also privileged. If you are not the intended recipient, please notify the sender and delete all copies of this transmission along with any attachments immediately. You should not copy or use it for any purpose, nor disclose its contents to any other person. ----------------------------------------------------------------------- From cxk340 at case.edu Tue Mar 3 17:15:07 2020 From: cxk340 at case.edu (Chaitanya Kolluru) Date: Tue, 3 Mar 2020 18:15:07 -0500 Subject: [Histonet] Looking for a sliding microtome Message-ID: Hi, I'm searching online for a microtome where the sample is fixed and the knife is moving. The microtome cutting movement needs to be motorized and automatic. Is there a microtome that has this functionality or is this something that simply does not exist? Thank you! -- Chaitanya From Histology at nwlabs.co.uk Wed Mar 4 12:35:18 2020 From: Histology at nwlabs.co.uk (NWL - Histology) Date: Wed, 4 Mar 2020 18:35:18 +0000 Subject: [Histonet] Histonet Digest, Vol 196, Issue 3 In-Reply-To: References: Message-ID: Re: Histonet digest Incomplete sectioning of blocks. I had to manage this situation a few year back and found that the best place to capture this is at the waterbath. QC reviews I.e numbers of return slides categorised into fault type can be collated over a month and displayed easily to a team of people. This data can also be a useful KPI of your labs processes. You can then use as evidence if you had to go down the route of a capability process with a single member of staff. Do you microscopically check each slide that is sent out? Cheers Stuart Beaver Head of Histology Nationwide Laboratories Poulton-le-Fylde UK Get Outlook for iOS ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Wednesday, March 4, 2020 6:00:02 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 196, Issue 3 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From albert.dotson at duke.edu Wed Mar 4 12:40:36 2020 From: albert.dotson at duke.edu (Bert Dotson) Date: Wed, 4 Mar 2020 18:40:36 +0000 Subject: [Histonet] Looking for a sliding microtome In-Reply-To: References: Message-ID: Sounds like Fisher Thermo HM450. I think these are still made. Still on their web site at least. Regards, Bert -----Original Message----- From: Chaitanya Kolluru Sent: Tuesday, March 3, 2020 6:15 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Looking for a sliding microtome Hi, I'm searching online for a microtome where the sample is fixed and the knife is moving. The microtome cutting movement needs to be motorized and automatic. Is there a microtome that has this functionality or is this something that simply does not exist? Thank you! -- Chaitanya From SteveM at mcclainlab.com Wed Mar 4 13:04:18 2020 From: SteveM at mcclainlab.com (Steve McClain) Date: Wed, 4 Mar 2020 19:04:18 +0000 Subject: [Histonet] Histonet Digest, Vol 196, Issue 3 Incomplete sectioning Message-ID: Two methods to avoid incomplete sectioning are: 1) ink the specimens well, using acetic acid (vinegar) to fix the ink, thereby making the ink easier to see in the block 2) have a microscope or 10 x lens adjacent to the microtome to allow for visual inspection. One of my histotechs used an old (really old- had a mirror for a light source) for years. Gradually as scopes are upgraded, the 'retired microscopes can be put to use in the lab. It is unfair to the histotechs that their work is judged by pathologists w microscopes, when the histotechs do not have access to the same tool, but life is not fair. Thanks, Steve A. McClain, MD McClain Labs 45 Manor Road Smithtown, NY 11787 631 361-4000 cell 631 926-3655 -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Wednesday, March 4, 2020 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 196, Issue 3 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: [EXTERNAL] Re: Incomplete cross sections of all tissue in blocks (Perl, Alison) 2. Re: Incomplete sectioning (Terri Braud) 3. Cutting benchmarks (Hagon, Christopher (Health)) 4. Looking for a sliding microtome (Chaitanya Kolluru) ---------------------------------------------------------------------- Message: 1 Date: Tue, 3 Mar 2020 18:36:12 +0000 From: "Perl, Alison" To: 'John Garratt' , Amy Self , 'John Garratt via Histonet' Subject: Re: [Histonet] [EXTERNAL] Re: Incomplete cross sections of all tissue in blocks Message-ID: <96e787bcf5684af6bb3b701beb77202a at MK-EXMB02.mkmg.com> Content-Type: text/plain; charset="utf-8" Each of our techs is responsible for fully facing their blocks and getting a representative section. If the docs see something is amiss (epidermis missing, incomplete cross section, etc), they can order a Technical Recut rather than a routine Recut. Unfortunately, it sounds like you (or someone else of authority), has to give some feedback/coaching that the sections are insufficient, particularly if it's a recurring problem. Is it one tech that needs a 1-on-1 convo, or a meeting with the whole staff to address widespread issues? Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at caremount.com -----Original Message----- From: John Garratt via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, March 3, 2020 12:02 PM To: Amy Self; histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Incomplete cross sections of all tissue in blocks I suggest that each histotech is responsible for the blocks they cut and they cut the deepers on the their own blocks when they are requested. With feedback on the reason for the deeper from the pathologists they (the techs) will become more confident and learn how deep to cut. John On Tue, Mar 3, 2020 at 7:31 AM, Amy Self via Histonet wrote: > Good Morning HistoNetters, > > I am reaching out to the histonet in hopes to get some suggestions from you on how to handle incomplete cross-sections of tissue in blocks. We are a small lab so this has not been an issue in the past but now that we are growing and our staff has increased I am getting feed-back from pathologist that the sections of tissue are not complete. They are asking for too many deepers that possibly could be avoided if it was cut deep enough to begin with. I have been given some managerial type duties ? which I don?t like cause I know nothing about managing people and I need to approach this but I need to approach this issue correctly. Do you have the histotech compare his/her cut slides to the block to make sure that a complete cross-section is obtained and is this documented somehow? Any and all suggestions I need. > > Thanks in advance for your help and as always you all rock.. ? > > Amy Self > Histology Lab Senior Tech > Lab > Tidelands Georgetown Memorial Hospital > 606 Black River Road > Georgetown, SC 29440 > (843) 520-8711 > ASelf at tidelandshealth.org > Our mission: We help people live better lives through better health. > > NOTE: > The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 3 Mar 2020 20:46:17 +0000 From: "Terri Braud" To: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] Incomplete sectioning Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B77C52 at HRHEX02-HOS.holyredeemer.local> Content-Type: text/plain; charset="us-ascii" A quality check can be accomplished in 2 places. It can be done at cutting, but it should already be being done and it doesn't seem to be working. Ideally, the stained H&E should be checked against the block face as it is pulled from the coverslipper to be given to the pathologist. Then it can be handed immediately to the tech that made the original error to "do over" Also, a common excuse will be "it was embedded poorly". The answer to that is that it is the cutting tech's responsibility to hand back a poorly embedded block for it to be re-embedded if they feel that they can't get a representation section. Remember, it could also be poorly cut gross, too. Then you can nip it in the bud before the slide reaches the pathologist, and you can quickly identify who is turning out inferior sections and counsel them appropriately if needed. Hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Tuesday, March 03, 2020 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 196, Issue 2 CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links or opening attachments. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Incomplete cross sections of all tissue in blocks (Amy Self) 2. Re: Incomplete cross sections of all tissue in blocks (John Garratt) ---------------------------------------------------------------------- Message: 1 Date: Tue, 3 Mar 2020 15:31:59 +0000 From: Amy Self To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Incomplete cross sections of all tissue in blocks Message-ID: Content-Type: text/plain; charset="utf-8" Good Morning HistoNetters, I am reaching out to the histonet in hopes to get some suggestions from you on how to handle incomplete cross-sections of tissue in blocks. We are a small lab so this has not been an issue in the past but now that we are growing and our staff has increased I am getting feed-back from pathologist that the sections of tissue are not complete. They are asking for too many deepers that possibly could be avoided if it was cut deep enough to begin with. I have been given some managerial type duties ? which I don?t like cause I know nothing about managing people and I need to approach this but I need to approach this issue correctly. Do you have the histotech compare his/her cut slides to the block to make sure that a complete cross-section is obtained and is this documented somehow? Any and all suggestions I need. Thanks in advance for your help and as always you all rock.. ? Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 (843) 520-8711 ASelf at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ------------------------------ Message: 2 Date: Tue, 03 Mar 2020 17:01:55 +0000 From: John Garratt To: Amy Self , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Incomplete cross sections of all tissue in blocks Message-ID: Content-Type: text/plain; charset=UTF-8 I suggest that each histotech is responsible for the blocks they cut and they cut the deepers on the their own blocks when they are requested. With feedback on the reason for the deeper from the pathologists they (the techs) will become more confident and learn how deep to cut. John On Tue, Mar 3, 2020 at 7:31 AM, Amy Self via Histonet wrote: > Good Morning HistoNetters, > > I am reaching out to the histonet in hopes to get some suggestions from you on how to handle incomplete cross-sections of tissue in blocks. We are a small lab so this has not been an issue in the past but now that we are growing and our staff has increased I am getting feed-back from pathologist that the sections of tissue are not complete. They are asking for too many deepers that possibly could be avoided if it was cut deep enough to begin with. I have been given some managerial type duties ? which I don?t like cause I know nothing about managing people and I need to approach this but I need to approach this issue correctly. Do you have the histotech compare his/her cut slides to the block to make sure that a complete cross-section is obtained and is this documented somehow? Any and all suggestions I need. > > Thanks in advance for your help and as always you all rock.. ? > > Amy Self > Histology Lab Senior Tech > Lab > Tidelands Georgetown Memorial Hospital > 606 Black River Road > Georgetown, SC 29440 > (843) 520-8711 > ASelf at tidelandshealth.org > Our mission: We help people live better lives through better health. > > NOTE: > The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 196, Issue 2 **************************************** ------------------------------ Message: 3 Date: Tue, 3 Mar 2020 21:21:08 +0000 From: "Hagon, Christopher (Health)" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Cutting benchmarks Message-ID: Content-Type: text/plain; charset="us-ascii" UNCLASSIFIED Hello Histonetters, Just wondering if anyone has had any benchmarks put in place in regards to blocks cut or slides produced per histotech/scientist per day? I realise that there are differing levels of complexity per block, we range from 1 slide per block to 21 one micron sections per renal block. Has any lab has WHS involved as far as Repetitive Strain Injuries are concerned? I'm not looking for any definitive answers that I'll hold anyone to, just if anyone has expectations of maximum throughput. We have a tracking system that we can accurately see how much work each person is doing. There seem to be limits on how many cases a pathologist can report in a day (again, varying levels of complexity) but what about for lab staff? Any thoughts greatly appreciated. Chris Hagon | Senior Scientist, Anatomical Pathology Phone: (02) 5124 2874 | Email: christopher.hagon at act.gov.au ACT Pathology | Canberra Health Services | ACT Government Canberra Hospital | Building 10 Level 1 | PO Box 11, Woden ACT 2606 | health.act.gov.au ----------------------------------------------------------------------- This email, and any attachments, may be confidential and also privileged. If you are not the intended recipient, please notify the sender and delete all copies of this transmission along with any attachments immediately. You should not copy or use it for any purpose, nor disclose its contents to any other person. ----------------------------------------------------------------------- ------------------------------ Message: 4 Date: Tue, 3 Mar 2020 18:15:07 -0500 From: Chaitanya Kolluru To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Looking for a sliding microtome Message-ID: Content-Type: text/plain; charset="UTF-8" Hi, I'm searching online for a microtome where the sample is fixed and the knife is moving. The microtome cutting movement needs to be motorized and automatic. Is there a microtome that has this functionality or is this something that simply does not exist? Thank you! -- Chaitanya ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 196, Issue 3 **************************************** From andrea at ka-recruiting.com Wed Mar 4 18:18:03 2020 From: andrea at ka-recruiting.com (Andrea Costello) Date: Wed, 4 Mar 2020 19:18:03 -0500 Subject: [Histonet] Latest Histology Positions Update Message-ID: Hi Histonetters - I just wanted to reach out and see if you (or someone you know) might be interested in a new permanent bench or management level histology position? Here is a list of our latest openings: *Florida - Histology Manager * *Georgia- Histology Manager * *Michigan - Group Lead Histology* *North Dakota - Lead Histotech* *New York- Grosser* *New York- Histotech* *New York - IHC Supervisor* *New York - Histology Manager* *New York - Anatomic Pathology Manager* *Ohio - Histotech* *Oregon - Experienced Histotech (IHC experience preferred) * *Tennessee- Grossing Supervisor (Nights)* My clients range from state of the art private laboratories to some of the top hospitals in the country! All of our clients are all offering aggressive compensation packages. If you are interested call Andrea at 617-922-2939 or andrea at ka-recruitng.com! Andrea Costello Client Relationship Manager Senior Healthcare Recruiter K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 *P: (617) 746-2745* *F: (617) 507-8009 * andrea at ka-recruiting.com From Carole.Johnson at uchealth.org Thu Mar 5 13:18:54 2020 From: Carole.Johnson at uchealth.org (Johnson, Carole) Date: Thu, 5 Mar 2020 19:18:54 +0000 Subject: [Histonet] Cutting benchmarks Message-ID: There is an extensive article in Annals of Diagnostic Pathology by Rene Buesa which is a little on the old side (2010) but still good. You can find it at www.sciencedirect.com title is Staffing benchmarks for histology laboratories. Hope this helps. Carole L Johnson, HT(ASCP)cm, QIHC(ASCP)cm Histotechnologist UCH/Memorial Central Hospital Histology Laboratory 1400 Boulder Street Colorado Springs, CO O 719.365.5204 carole.johnson at uchealth.org CONFIDENTIALITY NOTICE: This message and any attachments may contain privileged and confidential peer review, risk management, and/or quality management information pursuant to the Colorado Professional Review Act, C.R.S. 12-36.5-101, et seq., the Colorado Hospital Licensing law, C.R.S. 25-3-109, the Quality Management Programs law, C.R.S. 25-35-904, and other corresponding provisions of federal and state law. Please maintain the strict confidentiality of this information. If you are not the intended recipient, you are notified that any disclosure, copying, distribution, electronic storage or use of this communication is prohibited. If you received this communication in error, please notify us immediately by e-mail, attaching the original message, and delete the original message from your computer and any network to which your computer is connected. From mtighe at trudeauinstitute.org Thu Mar 5 15:17:54 2020 From: mtighe at trudeauinstitute.org (Mike Tighe) Date: Thu, 5 Mar 2020 21:17:54 +0000 Subject: [Histonet] hard Tick histology Message-ID: Hello, I was wondering if someone might have some expertise on how to prepare a hard-tick for sectioning in paraffin. I have been decalcifying to soften the exoskeleton but not sure if that even helps. I have used acid decalcifying but would EDTA be more appropriate? I have punctured the ticks and used vacuum for all steps (fixation, decalcification, dehydration etc....). Still crunchy on the outside and soft in the middle! Any help would be greatly appreciated!! Thanks! Mike From paula at excaliburpathology.com Thu Mar 5 15:53:35 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Thu, 5 Mar 2020 21:53:35 +0000 (UTC) Subject: [Histonet] hard Tick histology In-Reply-To: References: Message-ID: <1596193495.390857.1583445215168@mail.yahoo.com> The exoskeletons of insects are made of chitin and contain little calcium. Lysol spray contains a type of phenol which will soften chitin. The phenol is what kills bacteria, mold, and viruses by destroying their outer shells. Try spraying Lysol in a small container so you have a few mls. It will not evaporate. Soak the tick in this for about 10-20 minutes. Instead, you may have a bottle of phenol sitting around somewhere. Or?DIAPHANOL?(a 50 per cent solution of glacial acetic acid saturated with chlorine dioxide) is also used, but can also damage he internal tissue. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, March 5, 2020, 03:26:47 PM CST, Mike Tighe via Histonet wrote: Hello, I was wondering if someone might have some expertise on how to prepare a hard-tick for sectioning in paraffin. I have been decalcifying to soften the exoskeleton but not sure if that even helps. I have used acid decalcifying but would EDTA be more appropriate? I have punctured the ticks and used vacuum for all steps (fixation, decalcification, dehydration etc....). Still crunchy on the outside and soft in the middle! Any help would be greatly appreciated!! Thanks! Mike _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Thu Mar 5 18:37:34 2020 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 5 Mar 2020 19:37:34 -0500 Subject: [Histonet] Incomplete cross sections of all tissue in blocks In-Reply-To: References: Message-ID: Steve A. McClain, MD at McClain Labs in Smithtown NY notes: >>...to avoid incomplete sectioning...: ink the specimens well, using acetic acid (vinegar) to fix the ink, thereby making the ink easier to see in the block.<< I don't like ink for this purpose, because it clutters up the microscopic section and interferes with photography. Better to use safranin O for this purpose (not fluorescent like eosin Y) - it isn't visible in the sections. The safranin solution used as a counterstain in the microbiology department's Gram stain works quite well. When I requested recuts for any reason, I'd always fetch the paraffin block myself and examine it. Saved many surprises. Bob Richmond Samurai Pathologist Maryville TN From redrose297 at gmail.com Fri Mar 6 02:26:38 2020 From: redrose297 at gmail.com (warda hassan) Date: Fri, 6 Mar 2020 12:26:38 +0400 Subject: [Histonet] Tissue processing Message-ID: Hello to all histonets Advanced thanking to whoever will give me a feedback on their selection for tissue processing We are to purchase a new tissue processing List are Histo pro 200 & 300( histoline) Milstone Diapth Shandon - Thermo Revos Sakura VIP TEK6 Any feedback on your hand-on experiences with these system will be highly appreciated Manythanks Rose Histo-Incharge Royal Hospital Oman From Valerie.Hannen at parrishmed.com Fri Mar 6 06:17:15 2020 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Fri, 6 Mar 2020 12:17:15 +0000 Subject: [Histonet] FW: [EXTERNAL Sender] Tissue processing In-Reply-To: References: Message-ID: <62b56d4440b0404fa760a1120435ce4e@PMSVRLEX01.parrishmed.local> -----Original Message----- From: Hannen, Valerie Sent: Friday, March 06, 2020 5:54 AM To: 'warda hassan' Subject: RE: [EXTERNAL Sender] [Histonet] Tissue processing I have always loved my VIP's, I am in the process of trying to get a new one this new fiscal year. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: warda hassan via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 06, 2020 3:27 AM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL Sender] [Histonet] Tissue processing This message came from an external source. Please do not click links or open attachments if unexpected or unusual. Begin Original Message: ---------------------------------------------------------------------- Hello to all histonets Advanced thanking to whoever will give me a feedback on their selection for tissue processing We are to purchase a new tissue processing List are Histo pro 200 & 300( histoline) Milstone Diapth Shandon - Thermo Revos Sakura VIP TEK6 Any feedback on your hand-on experiences with these system will be highly appreciated Manythanks Rose Histo-Incharge Royal Hospital Oman _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PaPH0eDnAlaiSnq7Yv-U!otxOz97nMNfuilgfR5Kdqr1mnQKtyYq03rKeQvEmnQ7A4WOSpx5OBdcZwFMD0IoakMI6Qg$ From patpxs at gmail.com Fri Mar 6 08:16:28 2020 From: patpxs at gmail.com (Patpxs) Date: Fri, 6 Mar 2020 06:16:28 -0800 Subject: [Histonet] Tissue processing In-Reply-To: References: Message-ID: I favor the VIP too. My lab has 4 VIPs and 1 Leica Peloris. The VIPs can process more cassettes on a run and cost a lot less than the Peloris. Paula Sent from my iPhone > On Mar 6, 2020, at 12:34 AM, warda hassan via Histonet wrote: > > ?Hello to all histonets > > Advanced thanking to whoever will give me a feedback on their selection for > tissue processing > We are to purchase a new tissue processing > List are > Histo pro 200 & 300( histoline) > Milstone > Diapth > Shandon - Thermo Revos > Sakura VIP TEK6 > > Any feedback on your hand-on experiences with these system will be > highly appreciated > > Manythanks > > Rose > Histo-Incharge > Royal Hospital > Oman > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward at wakehealth.edu Fri Mar 6 09:19:55 2020 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Fri, 6 Mar 2020 15:19:55 +0000 Subject: [Histonet] Mounting media for immunofluorescence slides Message-ID: This may be an odd request but I am hoping someone out there can help us with this problem. Our institution is purchasing new digital scanners (Olympus 200) to scan all our pathology case slides. It has come to our attention that the mounting media we have all been using for cover slipping our direct immunofluorescence slides (skins and renal biopsies using FITC IgG, etc. antibodies) is incompatible with the use of these new scanners. We use a hard set mounting media from Vector but it is never quite hard enough to prevent any movement of the cover slip, which is apparently critical to the scanning process and potentially damaging to the scanner. The techs that are using the scanners are advising us to use a permanent mounting media such as Cytoseal 60. Has anyone encountered this problem and was there any downside to using something like Cytoseal 60 on the slides? We plan to test a few control slides to see how they look. Thanks in advance for any advice you can give me. Martha Ward Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157 From tina.vanmeter at gmail.com Fri Mar 6 09:26:59 2020 From: tina.vanmeter at gmail.com (Tina Van Meter) Date: Fri, 6 Mar 2020 10:26:59 -0500 Subject: [Histonet] Mounting media for immunofluorescence slides In-Reply-To: References: Message-ID: ProLong Gold - with or without DAPI On Fri, Mar 6, 2020 at 10:23 AM Martha Ward-Pathology via Histonet < histonet at lists.utsouthwestern.edu> wrote: > This may be an odd request but I am hoping someone out there can help us > with this problem. Our institution is purchasing new digital scanners > (Olympus 200) to scan all our pathology case slides. It has come to our > attention that the mounting media we have all been using for cover slipping > our direct immunofluorescence slides (skins and renal biopsies using FITC > IgG, etc. antibodies) is incompatible with the use of these new scanners. > We use a hard set mounting media from Vector but it is never quite hard > enough to prevent any movement of the cover slip, which is apparently > critical to the scanning process and potentially damaging to the scanner. > The techs that are using the scanners are advising us to use a permanent > mounting media such as Cytoseal 60. Has anyone encountered this problem > and was there any downside to using something like Cytoseal 60 on the > slides? We plan to test a few control slides to see how they look. > > Thanks in advance for any advice you can give me. > > Martha Ward > Molecular Diagnostics Lab > Wake Forest Baptist Health > Winston-Salem, NC 27157 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From galinadeyneko at yahoo.com Fri Mar 6 13:02:33 2020 From: galinadeyneko at yahoo.com (Galina Deyneko) Date: Fri, 6 Mar 2020 19:02:33 +0000 (UTC) Subject: [Histonet] Glycogen detection In-Reply-To: References: Message-ID: <1184515399.1307014.1583521353976@mail.yahoo.com> Dear Colleagues.Does anybody have experience how fix the tissues for successful glycogen ? detection in murine and humane cardiomyocytes.I am wondering maybe the trace of methanol in 10% formalin will dissolve glycogen?? What would be better process for paraffin embedding or use OCT embedding without fixation. Of course I prefer FFPE blocks, since OCT blocks give bad morphology.Any advices?Great thank you in advance Galina Deyneko From rsrichmond at gmail.com Sat Mar 7 12:47:33 2020 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 7 Mar 2020 13:47:33 -0500 Subject: [Histonet] Glycogen detection In-Reply-To: References: Message-ID: Galina Deyneko (where? asks: >>Does anybody have experience how fix the tissues for successful glycogen ? detection in murine and humane cardiomyocytes. I am wondering maybe the trace of methanol in 10% formalin will dissolve glycogen?? - What would be better process for paraffin embedding or use OCT embedding without fixation? Of course I prefer FFPE blocks, since OCT blocks give bad morphology.<< Ordinary neutral buffered formalin and paraffin embedding should be adequate. R.D. Lillie (3rd ed.) notes good results with Carnoy's fixative, alcoholic formalin, and acetic alcoholic formalin also. The traditional stain for glycogen is periodic acid Schiff (PAS). You verify the presence of glycogen by doing the stain with and without amylase ("diastase") predigestion. (A crude but adequate source of amylase is to just spit on the slide.) Bob Richmond Samurai Pathologist Maryville, Tennessee From jkiernan at uwo.ca Sun Mar 8 00:34:55 2020 From: jkiernan at uwo.ca (John Kiernan) Date: Sun, 8 Mar 2020 06:34:55 +0000 Subject: [Histonet] Glycogen detection; also Spring Forward In-Reply-To: References: , Message-ID: Glycogen (MW about 1,000,000) is soluble in water but insoluble in alcohol (Merck Index 12th ed.,1996, p.766). For this reason, non-aqueous coagulant fixatives may have advantages, especially for small specimens or thin layers of cultured cells. Fixation immobilizes cytoplasmic proteins, which then entangle the big long polysacccharide molecules of glycogen, keeping them approximately in their intracellular positions. Formaldehyde penetrates specimens rapidly, but its chemical reactions with proteins, especially the cross-linking that stabilizes structure, are slow (meaning 12-48 hours). During this time, the glycogen in liver cells dissolves and is carried in solution along the direction of the fixative diffusing into the specimen. In each hepatocyte, this intracellular diffusion of glygogen is stopped by each hepatocyte's cell membrane, which has a lipid layers that are unchanged by an aqueous formalin solution. As a result, the stainable glygogen piles up in the side of each hepatocyte furthest from the surface of the specimen. This artifact is often called "polarix=zarion". With processing into paraffin, which removes lipids and coagulates any proteins not yet made insoluble by formaldehyde, glycogen is anchored into place by fixed cytoplasmic proteins, but it can still be attacked and removed by amylase/diastase/spittle. All this has been known for at least 60 years. It's in the textbooks, as Bob Richmond pointed out yesterday. (Or was it the day before?) It's now time for us all to advance our clocks by an hour, go to bed and wake up in time for Church on Sunday! John Kiernan = = = ________________________________ From: Bob Richmond via Histonet Sent: 07 March 2020 13:47 To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Glycogen detection Galina Deyneko (where? asks: >>Does anybody have experience how fix the tissues for successful glycogen ? detection in murine and humane cardiomyocytes. I am wondering maybe the trace of methanol in 10% formalin will dissolve glycogen?? - What would be better process for paraffin embedding or use OCT embedding without fixation? Of course I prefer FFPE blocks, since OCT blocks give bad morphology.<< Ordinary neutral buffered formalin and paraffin embedding should be adequate. R.D. Lillie (3rd ed.) notes good results with Carnoy's fixative, alcoholic formalin, and acetic alcoholic formalin also. The traditional stain for glycogen is periodic acid Schiff (PAS). You verify the presence of glycogen by doing the stain with and without amylase ("diastase") predigestion. (A crude but adequate source of amylase is to just spit on the slide.) Bob Richmond Samurai Pathologist Maryville, Tennessee _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Sun Mar 8 13:40:10 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sun, 8 Mar 2020 18:40:10 +0000 Subject: [Histonet] Glycogen detection Message-ID: Q has been answered: fix in "10% Formalin" made up from concentrated liquid which has 10% Alcohol to retard reformation of paraformaldehyde polymers. Once the 40% liquid has been diluted to 4% the fixative effects of alcohol are minimised. Alcohol does not dissolve glycogen ( otherwise the dehydration stage of Pwax processing would) The Main Man JK has OK'd this. I am glad that he still posts/passes on his extensive knowledge! I can see further by having his shoulders to stand on.... Glycogen is still demonstrable ( with PAS) but, as JK pointed out, the Formalin will "push" the glycogen up to the plasma membrane as it fixes the proteins, giving what I learnt to call "streaming" artefact In 2014, I posted an image in Histonet Images of glycogen streaming in a FFPWS skin section stained with PAS. Have a look? Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From galinadeyneko at yahoo.com Sun Mar 8 15:23:13 2020 From: galinadeyneko at yahoo.com (Galina Deyneko) Date: Sun, 8 Mar 2020 20:23:13 +0000 (UTC) Subject: [Histonet] Glycogen detection References: <1952280785.2137289.1583698993215.ref@mail.yahoo.com> Message-ID: <1952280785.2137289.1583698993215@mail.yahoo.com> Dear Colleagues,I really appreciate your prompt and detailed responses to my question about glycogen detection. Thank you for jolting my memory, I think I'm starting to forget the biochemistry basics. I'm gonna review the histochemical textbooks. Your advice is very helpful and I will definitely follow the suggestions. Tank you again! Sincerely, Galina Deyneko From relia1 at earthlink.net Mon Mar 9 12:00:31 2020 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 9 Mar 2020 13:00:31 -0400 Subject: [Histonet] RELIA HOT Histology Job Alert! Full time Permanent Florida Opportunities. Message-ID: <000001d5f634$3e839240$bb8ab6c0$@earthlink.net> Hi Histonetters! I hope this is the start to a great week for you and let me start by wishing you a Happy Histotechnology Professionals Day - 1 day early! * tomorrow is the 10th anniversary of Histotechnology Professionals Day! I also wanted to let you know about some exciting opportunities I am working on in Florida in the Tampa Bay area. I am in need of a supervisor/lead tech and a histology tech. Both of these positions are Full-time, Permanent, M-F, DAYS. Florida license is required and new grads will be considered for the tech position. For more information please contact Pam Barker at 407-353-5070 or relia1 at earthlink.net or toll free 866-607-3542 Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From relia1 at earthlink.net Tue Mar 10 11:48:41 2020 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 10 Mar 2020 12:48:41 -0400 Subject: [Histonet] Happy Histotechnology Professionals Day! #lovemyhistopeeps Message-ID: <085001d5f6fc$5c7a7b60$156f7220$@earthlink.net> Happy Histotechnology Professionals Day!! >From Pam Barker & RELIA Solutions Kudos to you. For everything you do. Saving Lives Every Day! Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From Kim.Kolman at va.gov Tue Mar 10 14:15:39 2020 From: Kim.Kolman at va.gov (Kolman, Kimberly D.) Date: Tue, 10 Mar 2020 19:15:39 +0000 Subject: [Histonet] room temperature in Histology labs Message-ID: Hello All; Can anyone suggest a required temperature range for Histology Labs? Specifically in regards to performing paraffin embedding and microtomy? I think room temperature swings of 66 to 83 degrees F are excessive and I am seeing some issues with paraffin cooling too quickly while embedding as well as my paraffin ribbons blowing around before I get them to the water bath. Engineering insists the temps and air speed are within their "required range" (according to the thermostat on the wall..........). Thanks for your input. Kim Kimberly D. Kolman, HT (ASCP) VA Eastern Kansas Health Care System Dwight D. Eisenhower VA Medical Center 4101 S. 4th St. Trfwy. Leavenworth, Ks 66048 913-682-2000 x 52537 Kim.Kolman at va.gov From relia1 at earthlink.net Wed Mar 11 10:25:14 2020 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 11 Mar 2020 11:25:14 -0400 Subject: [Histonet] RELIA Special Bulletin for Histology Leadership!! Message-ID: <000001d5f7b9$431d9400$c958bc00$@earthlink.net> Hello Histonetters, How are you? I hope you are having a wonderful day. I have several histology leadership positions and I need your help. I am currently working with clients in: ? Georgia ? California ? Florida* ? Wyoming ? Tennessee My clients are in need of exceptional histology professionals for a variety of leadership roles. My question is do you know of anyone who might be interested in management positions in any of these areas? Or Histonetters, Would you be interested in an opportunity in one of these areas? Incidentally, some of my clients are willing to consider lead techs ready to step up to supervisor! *The Florida Supervisor License is required for the Florida position but it is easy to get!!* I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542, on my cell at 407-353-5070 or relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From mpence at grhs.net Wed Mar 11 15:44:25 2020 From: mpence at grhs.net (Michael S Pence) Date: Wed, 11 Mar 2020 20:44:25 +0000 Subject: [Histonet] Validation of Tissue Processor Message-ID: When I am looking for answers I go to the experts. Who has written a procedure for validation of a new tissue processor and would be willing to share? I just want to make sure I have not overlooked something. Thanks for the help, Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 mpence at grhs.net | www.greatrivermedical.org. www.Facebook.com/GreatRiverHealthSystems | www.Twitter/GreatRiverMed Information in this communication, including attachments, is confidential and intended only for the addressee(s). This communication may contain privileged, confidential, proprietary or trade secret information entitled to protection or exemption from disclosure under law. If you are not an intended recipient, please know that any use, distribution or copying of this communication, or any action taken based on the information in this communication, is unauthorized and may be unlawful. If you received this communication in error, please notify the sender and delete this communication from your device. From c.tague at pathologyarts.com Wed Mar 11 16:54:06 2020 From: c.tague at pathologyarts.com (Curt Tague) Date: Wed, 11 Mar 2020 21:54:06 +0000 Subject: [Histonet] New processor and white paper, need pathologists to participate Message-ID: Hello histo-world, I've helped a company develop a new tissue processor, a rapid technology which does NOT utilize microwaves and process fresh, fatty, breast and colon type tissue, beautifully, in under 5 hrs. We are ready to put it in the market but first want to do some kind of official validation and publish a white paper on the process. We are considering pathologist's who might be interested in participating in this process and having their names included in the paper. Without disclosing any of the proprietary specifics of the technology at this point, would any of you know of a pathologist who might be interested in this project? All the best! Curt From garreyf at gmail.com Wed Mar 11 19:07:11 2020 From: garreyf at gmail.com (Garrey Faller) Date: Wed, 11 Mar 2020 20:07:11 -0400 Subject: [Histonet] New processor and white paper, need pathologists to participate Message-ID: <19CC4BA5-1784-44B6-AA06-AC4D96FD1F1D@gmail.com> I might be interested Curt. Please send me details. Garrey Faller MD Sent from my iPhone > On Mar 11, 2020, at 6:04 PM, Curt Tague via Histonet wrote: > From criley at dpspa.com Thu Mar 12 08:14:20 2020 From: criley at dpspa.com (Charles Riley) Date: Thu, 12 Mar 2020 09:14:20 -0400 Subject: [Histonet] Tissue Tek embedding centers Message-ID: Has anyone experienced an issue with their embedding center where is had continuous power outages (machine starts to cycle on then power kicks out, then immediately restarts)? I am trying to determine what the issue is to see if it can be fixed easily or if I need to look at a service call or if better to just buy new (my machines are around 11 years old) -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From greg.dobbin at gmail.com Thu Mar 12 12:19:03 2020 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Thu, 12 Mar 2020 14:19:03 -0300 Subject: [Histonet] Tissue Tek embedding centers Message-ID: Hi Charles, If memory serves, there is a thermal fuse or switch that will shut the unit down if it exceeds a certain temp. Once the unit cools, the switch is reset. So either that switch needs to be replaced or there is a problem elsewhere that is causing the unit to overheat. Replacing that switch should be an easy and cost-effecient thing to try first. Good luck, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From criley at dpspa.com Thu Mar 12 12:27:06 2020 From: criley at dpspa.com (Charles Riley) Date: Thu, 12 Mar 2020 13:27:06 -0400 Subject: [Histonet] Tissue Tek embedding centers In-Reply-To: References: Message-ID: Thank you for your help! On Thu, Mar 12, 2020 at 1:19 PM Greg Dobbin wrote: > Hi Charles, > If memory serves, there is a thermal fuse or switch that will shut the > unit down if it exceeds a certain temp. Once the unit cools, the switch is > reset. So either that switch needs to be replaced or there is a problem > elsewhere that is causing the unit to overheat. Replacing that switch > should be an easy and cost-effecient thing to try first. > Good luck, > Greg > > -- > *Greg Dobbin* > 1205 Pleasant Grove Rd > RR#2 York, > PE C0A 1P0 > > > *Everything in moderation...even moderation itself**!* > -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From mashalsmom at gmail.com Thu Mar 12 13:46:58 2020 From: mashalsmom at gmail.com (Emily Horst) Date: Thu, 12 Mar 2020 14:46:58 -0400 Subject: [Histonet] Histo tech openings! Message-ID: Hey everyone! I have a Sr Histotech position and 2 Histotech positions open in Cincinnati, Ohio. If you would like more information please contact me. Emily Horst Histology Supervisor From criley at dpspa.com Fri Mar 13 11:51:32 2020 From: criley at dpspa.com (Charles Riley) Date: Fri, 13 Mar 2020 12:51:32 -0400 Subject: [Histonet] Pap slides Message-ID: Can pap slides be left in 95% alcohol overnight to be stained the next morning without affecting the quality of the slides? Or should they be moved to xylene then run back prior to staining? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From aperl at caremount.com Fri Mar 13 12:19:31 2020 From: aperl at caremount.com (Perl, Alison) Date: Fri, 13 Mar 2020 17:19:31 +0000 Subject: [Histonet] [EXTERNAL] Pap slides In-Reply-To: References: Message-ID: We routinely leave our ThinPrep Paps in 95 overnight and stain the next day. If I remember our Hologic training correctly, they said the slides can be left up to 5 days - best to check with an FAS before trying it, though Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at caremount.com -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 13, 2020 12:52 PM To: Histo List Subject: [EXTERNAL] [Histonet] Pap slides Can pap slides be left in 95% alcohol overnight to be stained the next morning without affecting the quality of the slides? Or should they be moved to xylene then run back prior to staining? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen at parrishmed.com Sat Mar 14 08:10:01 2020 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Sat, 14 Mar 2020 13:10:01 +0000 Subject: [Histonet] [EXTERNAL Sender] Pap slides In-Reply-To: References: Message-ID: Yes, they can be left overnight in 95% alcohol before staining. We do at times and have not had any report of altered quality. -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 13, 2020 12:52 PM To: Histo List Subject: [EXTERNAL Sender] [Histonet] Pap slides This message came from an external source. Please do not click links or open attachments if unexpected or unusual. Begin Original Message: ---------------------------------------------------------------------- Can pap slides be left in 95% alcohol overnight to be stained the next morning without affecting the quality of the slides? Or should they be moved to xylene then run back prior to staining? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PaPH0eDnAlaiSnq7Yv-U!t41b5SyZJvJtfmoB17JVt_hvFQ_Fgvuf42TYKsSF1BIUruucoQ1Qjmkduo-4wzUXruaX9w$ From tony.henwood at health.nsw.gov.au Sat Mar 14 15:57:52 2020 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sat, 14 Mar 2020 20:57:52 +0000 Subject: [Histonet] Fw: [EXTERNAL] Pap slides In-Reply-To: <1584138098824.7215@health.nsw.gov.au> References: , , <1584138098824.7215@health.nsw.gov.au> Message-ID: <1584219472472.51675@health.nsw.gov.au> We regularly leave smeared slides in 95% overnight and often over the weekend before staining. There have not been any morphological issues noted. Remember to seal the lid since alcohol will evaporate quite quickly. You will notice an increased eosinophilia in the dried upper area of the slide. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Perl, Alison via Histonet Sent: Saturday, 14 March 2020 04:19 To: 'Charles Riley via Histonet' Subject: Re: [Histonet] [EXTERNAL] Pap slides We routinely leave our ThinPrep Paps in 95 overnight and stain the next day. If I remember our Hologic training correctly, they said the slides can be left up to 5 days - best to check with an FAS before trying it, though Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at caremount.com -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 13, 2020 12:52 PM To: Histo List Subject: [EXTERNAL] [Histonet] Pap slides Can pap slides be left in 95% alcohol overnight to be stained the next morning without affecting the quality of the slides? Or should they be moved to xylene then run back prior to staining? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From shannonegower at gmail.com Sun Mar 15 13:10:25 2020 From: shannonegower at gmail.com (Shannon Gower) Date: Sun, 15 Mar 2020 14:10:25 -0400 Subject: [Histonet] Cutting room and social distancing Message-ID: <5249177C-426A-40A8-985A-E31E315F5AEF@gmail.com> Hello all, In my lab, the histology room is small with 4-5 people in close proximity to one another. Is anyone else considering modifying shifts to separate staff and reduce exposure in this crazy world of COVID? Trying to make the best plan while expecting every to change day to day. Any ideas on how to keep labs free of virus? Any input is appreciated Shannon Gower Histology Manager PathGroup Salem, VA Sent from my iPhone From paula at excaliburpathology.com Sun Mar 15 14:26:44 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Sun, 15 Mar 2020 19:26:44 +0000 (UTC) Subject: [Histonet] Cutting room and social distancing In-Reply-To: <5249177C-426A-40A8-985A-E31E315F5AEF@gmail.com> References: <5249177C-426A-40A8-985A-E31E315F5AEF@gmail.com> Message-ID: <1425531296.3460752.1584300404313@mail.yahoo.com> Yes, that is one of the CDCs' recommendations. Use different shifts to spread out people. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Sunday, March 15, 2020, 01:23:45 PM CDT, Shannon Gower via Histonet wrote: Hello all, In my lab,? the histology room is small with 4-5 people in close proximity to one another. Is anyone else considering modifying shifts to separate staff and reduce exposure in this crazy world of COVID? Trying to make the best plan while expecting every to change day to day. Any ideas on how to keep labs free of virus? Any input is appreciated Shannon Gower Histology Manager PathGroup Salem, VA Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dane.Hill at ttuhsc.edu Mon Mar 16 09:01:05 2020 From: Dane.Hill at ttuhsc.edu (Hill, Dane) Date: Mon, 16 Mar 2020 14:01:05 +0000 Subject: [Histonet] Frozen section slides fading In-Reply-To: References: <7798957db4994224b1ac5dd70d2353af@ttuhsc.edu>, , Message-ID: <2e7c06b856444fb98694517e972ae430@ttuhsc.edu> Hello all, Thank you, in advance, for your help and for the information you've already posted online. It is helpful. I am hoping you can help me troubleshoot an issue we are having. I am the Mohs fellow at Texas Tech dermatology. For the last 9 months or so we have been noticing that the longer our frozen section slides are away from the day of surgery, the more they develop these pink, amorphous spots in certain areas of the tissue. The further away from the surgery, the more faded they get. The actual tissue remains intact on the slide, with complete epidermis evaluable, but it's almost as if all the hematoxylin fades or something because it just turns into that bright pink color, but does not hold any of the purple. We have tried changing our xylene substitutes and even changed the hematoxylin and eosin stains. The thinner (5 micron cuts) are affected long before the 20 micron cuts. Photos were too big to be attached, but I can PM someone with them, if needed. They are from slides prepared just a few weeks ago and are just starting to fade. In about 6 months they will turn all pink. Interestingly, this is not happening on any of our permanent sections. They remain perfectly stained for years. And it will happen, even if we don't "bake" any of the slides or heat them in storage. The only thing the techs say they do differently for the frozen sections is use xylene substitute in place of xylene. They also mix a little xylene substitute in the glue to cover slip the slides. I wasn't sure if that could be contributing. If there are any testing protocols of specific reagents we use or adjustments in staining that have previously remedied the issue, I'd be very grateful as we have been unsuccessful at pinning it down. Thank you, again! Dane Hill From kdean70 at hotmail.com Mon Mar 16 09:01:44 2020 From: kdean70 at hotmail.com (Ken M) Date: Mon, 16 Mar 2020 14:01:44 +0000 Subject: [Histonet] Tissue Blocks Message-ID: Hello All: I have thousands of tissue blocks from a large US certified reference lab. Most of them were used for IHC diagnosis. I am looking to trade what I have for more common Histology blocks for control slides such as Mast Cell, Pneumocystis, Copper, Gram, Aspergillus fungus, H-Pylori, Spirochete, Acid Fast Bacteria and other common Histology tissue for special stain control slides. I also have lots of normal tissue blocks. Please let me know if you are interested. Ken Marzinsky 4122 Bennett Memorial Road Durham, NC 27712 713-822-0256 kdean70 at hotmail.com From Nancy.Lowen at va.gov Mon Mar 16 11:26:47 2020 From: Nancy.Lowen at va.gov (Lowen, Nancy) Date: Mon, 16 Mar 2020 16:26:47 +0000 Subject: [Histonet] clearing agents Message-ID: Good Morning, Was wondering what preferences are as to CitriSolv versus Clearite 3 or Propar 3? Process lots of bone tissue and wondering which one everyone thinks works best. Thanks Nancy From tony.henwood at health.nsw.gov.au Mon Mar 16 17:58:53 2020 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon, 16 Mar 2020 22:58:53 +0000 Subject: [Histonet] Frozen section slides fading In-Reply-To: <2e7c06b856444fb98694517e972ae430@ttuhsc.edu> References: <7798957db4994224b1ac5dd70d2353af@ttuhsc.edu>, , <2e7c06b856444fb98694517e972ae430@ttuhsc.edu> Message-ID: <5c937278da004457bcaab783bf9bfce6@SVDCMBX-MEX024.nswhealth.net> Hi Dane, Fading and especially leaching of the eosin, will occur if slides are incompletely dehydrated. Flip out the subsidiary condenser lens (or drop the condenser) and look for refractile water droplets. Certain mountants can also cause fading of H&Es. I would also be concerned about the xylene substitute. Alternatively, after staining and ethanol dehydration, air dry the slides (making sure they are completely dry) and directly coverslip with your mountant (without the substitute added). This will reduce the risk of xylene exposure. Hopefully this is useful Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Hill, Dane via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, 17 March 2020 1:01 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Frozen section slides fading Hello all, Thank you, in advance, for your help and for the information you've already posted online. It is helpful. I am hoping you can help me troubleshoot an issue we are having. I am the Mohs fellow at Texas Tech dermatology. For the last 9 months or so we have been noticing that the longer our frozen section slides are away from the day of surgery, the more they develop these pink, amorphous spots in certain areas of the tissue. The further away from the surgery, the more faded they get. The actual tissue remains intact on the slide, with complete epidermis evaluable, but it's almost as if all the hematoxylin fades or something because it just turns into that bright pink color, but does not hold any of the purple. We have tried changing our xylene substitutes and even changed the hematoxylin and eosin stains. The thinner (5 micron cuts) are affected long before the 20 micron cuts. Photos were too big to be attached, but I can PM someone with them, if needed. They are from slides prepared just a few weeks ago and are just starting to fade. In about 6 months they will turn all pink. Interestingly, this is not happening on any of our permanent sections. They remain perfectly stained for years. And it will happen, even if we don't "bake" any of the slides or heat them in storage. The only thing the techs say they do differently for the frozen sections is use xylene substitute in place of xylene. They also mix a little xylene substitute in the glue to cover slip the slides. I wasn't sure if that could be contributing. If there are any testing protocols of specific reagents we use or adjustments in staining that have previously remedied the issue, I'd be very grateful as we have been unsuccessful at pinning it down. Thank you, again! Dane Hill _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From tony.henwood at health.nsw.gov.au Mon Mar 16 18:18:36 2020 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon, 16 Mar 2020 23:18:36 +0000 Subject: [Histonet] Flippin on the Bond Message-ID: <4da252a21eff4eccb7ffe9c3e4bb11a3@SVDCMBX-MEX024.nswhealth.net> Hi all, I was reading an article entitled ?Evaluation of Tissue Adhesion and Staining Performance of BOND Adhesive Microscope Slides: A Comparative Study on the BOND-III and BOND-MAX Platforms? (https://pdfs.semanticscholar.org/f5f1/ad0741bc5445d0e1079a0d07a32c8a3a26c1.pdf) and they make mention of the ?Flippin Protocol?, an amended protocol widely used by BOND customers. It is used for those antibodies that are adversely affected by the endogenous peroxidase block that is used in the Bond kits (and possibly with other non-Bond kits as well). In this protocol, the peroxidase block is applied after the localisation antibody is applied. Examples given by Leica are PAX5 and AMACR. The paper refers to several other antibodies as well (CD3, CD34, SMA and TTF1) that uses the ?Flippin protocol?. Now I have not had issues with the first three antibodies (they work quite well with the standard protocol, ie peroxidase block before the localisation antibody) and have no experience with TTF1 (we are a pediatric laboratory). Has anyone experienced this issue with antibodies and use the Flippin protocol? What antibodies are an issue? Thanks for your input Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) | Principal Scientist; Adjunct Fellow, School of Medicine, University of Western Sydney; Visiting Lecturer, School of Life Sciences, Faculty of Science, University of Technology Sydney | Histopathology t: (02) 9845 3306 | e: tony.henwood at health.nsw.gov.au | w: www.schn.health.nsw.gov.au [http://res.schn.health.nsw.gov.au/signatures/facebook.gif] [http://res.schn.health.nsw.gov.au/signatures/twitter.gif] [http://res.schn.health.nsw.gov.au/signatures/chw.gif] Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia [http://res.schn.health.nsw.gov.au/signatures/shared_banner.jpg] ? Please consider the environment before printing this email. This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From LRaff at uropartners.com Fri Mar 20 12:58:19 2020 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 20 Mar 2020 17:58:19 +0000 Subject: [Histonet] Brief thoughts as the lab winds down--Haiku in the Time of COVID Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF129955C8@COLOEXCH01.uropartners.local> http://www.chicagonow.com/downsize-maybe/2020/03/covid-19-haiku/ Lester J. Raff, MD MBA FCAP Laboratory Director UroPartners LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 Telephone 708-486-0076 Fax 708-492-0203 From kdean70 at hotmail.com Tue Mar 24 15:00:15 2020 From: kdean70 at hotmail.com (Ken M) Date: Tue, 24 Mar 2020 20:00:15 +0000 Subject: [Histonet] Paraffin Tissue Blocks Message-ID: To anyone interested: Please don't throw your old tissue blocks in the trash after they age out after 10 years. Many of them can be used by researchers and Pathologists and are badly needed. If you don't want to sort them out, we will pay you a fair price for the whole lot as long as we have a list of what they are and your lab is properly certified. Ken Marzinsky 4122 Bennett Memorial Road Durham, NC 27712 713-822-0256 kdean70 at hotmail.com From relia1 at earthlink.net Wed Mar 25 11:07:02 2020 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 25 Mar 2020 12:07:02 -0400 Subject: [Histonet] How are you? Message-ID: <000001d602bf$6c3881e0$44a985a0$@earthlink.net> Hi Histonetters, How are you? I hope you and your loved ones are safe and healthy. I know things are really crazy right now - Chaotic even. I want to assure you. Things Will Get Better. This Will End. & We Will Return To Our Normal Lives. I am so proud to be able to say that the people I work with and their colleagues are the heroes, the front liners, the ones that are giving more than anyone else. THANK YOU!! For everything you do TODAY & EVERYDAY! Instead of my newsletter I am sharing a beautiful and inspirational picture created by Sara Paglia an Italian artist: https://reliasolutionspambarker.wordpress.com/2020/03/19/ilovemyhistopeepsjo bs4myhistopeepsi-love-you-guys-and-am-so-proud-of-you%f0%9f%a5%b0/ Is there anything I can do for you? Were you actively looking for a new position before this crisis? Are you someone who is unemployed as opposed to furloughed? Are you feeling lonely or isolated and just need to hear a friendly voice? Call Me, Text Me, Email Me, Or Contact Me through Social Media. We are all in this together. Looking forward to the light at the end of the tunnel. Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From Tom_Wells at bcit.ca Wed Mar 25 14:34:56 2020 From: Tom_Wells at bcit.ca (Tom Wells) Date: Wed, 25 Mar 2020 19:34:56 +0000 Subject: [Histonet] On-line references Message-ID: Given that our Institute's library is closed due to the pandemic, is anyone aware of on-line versions of Histotechnology/ Histochemistry textbooks? Thanks. Tom Tom Wells BSc, MEd, MLT, ART Faculty Medical Laboratory Science School of Health Sciences SW03-3088 (604) 412-7594 BCIT From llewllew at shaw.ca Wed Mar 25 15:28:17 2020 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Wed, 25 Mar 2020 13:28:17 -0700 Subject: [Histonet] On-line references In-Reply-To: References: Message-ID: StainsFile is still available at http://stainsfile.info In the downloads section there is a scanned, corrected and reformatted copy of the Microtomist's Vade Mecum, 7th edition. This text covers other areas as well as medical and is in the public domain in the US and Canada, and likely elsewhere. The problem with putting textbooks on line is that they must have their copyright expired and be in the public domain, which means they will invariably have been published in the first half of the 20th century, i.e. 50 or 60 years, or more, ago. Bryan Llewellyn Tom Wells via Histonet wrote: > Given that our Institute's library is closed due to the pandemic, is anyone aware of on-line versions of Histotechnology/ Histochemistry textbooks? Thanks. Tom > > Tom Wells BSc, MEd, MLT, ART > Faculty > Medical Laboratory Science > School of Health Sciences > SW03-3088 > (604) 412-7594 > BCIT > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Tom_Wells at bcit.ca Wed Mar 25 15:54:02 2020 From: Tom_Wells at bcit.ca (Tom Wells) Date: Wed, 25 Mar 2020 20:54:02 +0000 Subject: [Histonet] On-line references In-Reply-To: References: Message-ID: <09bca9c4d03d48dabf4ff45ff54a3b7b@bcit.ca> Thanks Bryan, Yes I refer to it all the time. I will add it to my list. Cheers. Tom Tom Wells BSc, MEd, MLT, ART Faculty Medical Laboratory Science School of Health Sciences SW03-3088 (604) 412-7594 BCIT -----Original Message----- From: Bryan Llewellyn Sent: March 25, 2020 1:28 PM To: Tom Wells ; Histonet Subject: Re: [Histonet] On-line references CAUTION: This email originated from outside of BCIT. Do not click links or open attachments unless you recognize the sender and know the content is safe. StainsFile is still available at https://can01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fstainsfile.info&data=02%7C01%7Ctom_wells%40bcit.ca%7C32033609df8e4a57bd9e08d7d0fd2638%7C8322cefd0a4c4e2cbde5b17933e7b00f%7C0%7C0%7C637207658731731714&sdata=W1j3zzBIs5PibQUNjeY%2FcnOFBTy%2BshfBkvQOXPM1m%2BU%3D&reserved=0 In the downloads section there is a scanned, corrected and reformatted copy of the Microtomist's Vade Mecum, 7th edition. This text covers other areas as well as medical and is in the public domain in the US and Canada, and likely elsewhere. The problem with putting textbooks on line is that they must have their copyright expired and be in the public domain, which means they will invariably have been published in the first half of the 20th century, i.e. 50 or 60 years, or more, ago. Bryan Llewellyn Tom Wells via Histonet wrote: > Given that our Institute's library is closed due to the pandemic, is > anyone aware of on-line versions of Histotechnology/ Histochemistry > textbooks? Thanks. Tom > > Tom Wells BSc, MEd, MLT, ART > Faculty > Medical Laboratory Science > School of Health Sciences > SW03-3088 > (604) 412-7594 > BCIT > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://can01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists > .utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Ctom_wells%40bcit.ca%7C32033609df8e4a57bd9e08d7d0fd2638%7C8322cefd0a4c4e2cbde5b17933e7b00f%7C0%7C0%7C637207658731731714&sdata=qtCSG4nto2RPGw9lD4ZmGhGg%2BMT2T260%2FTLvJB92YeA%3D&reserved=0 > From jkiernan at uwo.ca Thu Mar 26 01:11:32 2020 From: jkiernan at uwo.ca (John Kiernan) Date: Thu, 26 Mar 2020 06:11:32 +0000 Subject: [Histonet] On-line references In-Reply-To: References: Message-ID: Hello, Tom. Some old classics are there for free, most notably JR Baker's "Principles of Biological Microtechnique" (1958), but almost anything more recent has to be bought. There are plenty of cheap older editions of histotechnology books on sites like AbeBooks. Check it out for the last edition of Pearse's Histochemistry! I was amazed. Even the latest editions of books in our field cost only about $100 from the publisher and most are good for several years. Compare this with the price of a few drops of an antibody or (more realistically) a staining machine in which you must only use the liquids sold by its vendor. John Kiernan = = = ________________________________ From: Tom Wells via Histonet Sent: 25 March 2020 14:34 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] On-line references Given that our Institute's library is closed due to the pandemic, is anyone aware of on-line versions of Histotechnology/ Histochemistry textbooks? Thanks. Tom Tom Wells BSc, MEd, MLT, ART Faculty Medical Laboratory Science School of Health Sciences SW03-3088 (604) 412-7594 BCIT _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rford at hcmhcares.org Thu Mar 26 09:08:24 2020 From: rford at hcmhcares.org (Rhonda Ford) Date: Thu, 26 Mar 2020 10:08:24 -0400 Subject: [Histonet] Reagent alcohol Message-ID: Good morning. I was wondering if any of you all are having problems receiving reagent alcohol in this time of crisis? Hope not! We need to keep our tissue processors rolling! -- Rhonda Ford, Histology Lab Henry Community Health Main Campus New Castle, Indiana 47362 (765) 521-1148 -- *"We Make Lasting Connections"*Henry Community Health From Tom_Wells at bcit.ca Thu Mar 26 11:27:07 2020 From: Tom_Wells at bcit.ca (Tom Wells) Date: Thu, 26 Mar 2020 16:27:07 +0000 Subject: [Histonet] On-line references In-Reply-To: References: Message-ID: <6684f1c1f59e48dcaf800cd28f3617c9@bcit.ca> John, Thank you for your response. I should have been a little clearer in my original post. I was posting the question on behalf of one of my students. In the course that I teach I have chosen to not use a required textbook. I list several recommended texts, including yours. Many of the students do in fact buy the texts. However, many use the texts I have reserved in our school's library. Since our library is closed, I wondered which current texts had e-versions so that the students could buy those and have access immediately. I was also wanting to direct our library to purchase e versions to prevent this kind of bottleneck from ever happening in the future. I am familiar with all of the common physical textbooks, but, not so for the electronic versions. I wondered if there were particularly good electronic versions. Thanks. Tom Tom Wells BSc, MEd, MLT, ART Faculty Medical Laboratory Science School of Health Sciences SW03-3088 (604) 412-7594 BCIT From: John Kiernan Sent: March 25, 2020 11:12 PM To: histonet at lists.utsouthwestern.edu; Tom Wells Subject: Re: On-line references CAUTION: This email originated from outside of BCIT. Do not click links or open attachments unless you recognize the sender and know the content is safe. Hello, Tom. Some old classics are there for free, most notably JR Baker's "Principles of Biological Microtechnique" (1958), but almost anything more recent has to be bought. There are plenty of cheap older editions of histotechnology books on sites like AbeBooks. Check it out for the last edition of Pearse's Histochemistry! I was amazed. Even the latest editions of books in our field cost only about $100 from the publisher and most are good for several years. Compare this with the price of a few drops of an antibody or (more realistically) a staining machine in which you must only use the liquids sold by its vendor. John Kiernan = = = ________________________________ From: Tom Wells via Histonet > Sent: 25 March 2020 14:34 To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] On-line references Given that our Institute's library is closed due to the pandemic, is anyone aware of on-line versions of Histotechnology/ Histochemistry textbooks? Thanks. Tom Tom Wells BSc, MEd, MLT, ART Faculty Medical Laboratory Science School of Health Sciences SW03-3088 (604) 412-7594 BCIT _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From maxim_71 at mail.ru Thu Mar 26 13:15:44 2020 From: maxim_71 at mail.ru (=?UTF-8?B?0J/QtdGI0LrQvtCyINCc0LDQutGB0LjQvA==?=) Date: Thu, 26 Mar 2020 21:15:44 +0300 Subject: [Histonet] =?utf-8?q?On-line_references?= In-Reply-To: References: Message-ID: <1585246544.31926596@f497.i.mail.ru> Many old books are here as?e-versions: www.archive.org -- Maxim Peshkov Russia Taganrog ? From ewj at pigs.ag Thu Mar 26 15:24:22 2020 From: ewj at pigs.ag (E. Wayne Johnson) Date: Fri, 27 Mar 2020 04:24:22 +0800 Subject: [Histonet] On-line references In-Reply-To: <1585246544.31926596@f497.i.mail.ru> References: <1585246544.31926596@f497.i.mail.ru> Message-ID: <273ebbbf-be33-629f-b28e-9a894c2747ff@pigs.ag> And I appreciate Bryan Llewellyn and the other old experienced hands and I really like Gray and Humason and even Lillie, and the others and the way they wrote and the way they thought, and their delight and fascination with the world they were discovering. E. Wayne Johnson Enable AgTech Beijing ?????? ?????? via Histonet wrote: > Many old books are here as?e-versions: www.archive.org > -- > Maxim Peshkov > Russia > Taganrog > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at dpspa.com Fri Mar 27 07:07:20 2020 From: criley at dpspa.com (Charles Riley) Date: Fri, 27 Mar 2020 08:07:20 -0400 Subject: [Histonet] BRAF testing Message-ID: Can anyone send me a block that can be used to validate BRAF V600E antibody. I have tried several cases that should work but our pathologists don't think it is working correctly. I just need a small core sample that is known to be positive. Any help would greatly be appreciated -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From Richard.Cartun at hhchealth.org Fri Mar 27 09:05:51 2020 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 27 Mar 2020 14:05:51 +0000 Subject: [Histonet] BRAF testing In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2ED459D1C3@HHCEXCHMB03.hhcsystem.org> This is a very difficult target to detect. I was told recently by a vendor that one needs to use prolonged, very aggressive antigen retrieval (pressure cooker with high pH buffer) to get consistent results. Also, the type of cancer may influence your success (colon vs. thyroid vs. melanoma). I would be interested in knowing what others' experiences are. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 27, 2020 8:07 AM To: Histo List Subject: [Histonet] BRAF testing This is an email from an external source. USE CAUTION opening attachments or links from unknown senders. Can anyone send me a block that can be used to validate BRAF V600E antibody. I have tried several cases that should work but our pathologists don't think it is working correctly. I just need a small core sample that is known to be positive. Any help would greatly be appreciated -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!Oq9k97gGgRatvdlIoqX-QkrcMRMS19Iuxle4pZY1FqKfIdEBOVk2YOJ3yVO0qAn3MBg$ Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From john.garratt at ciqc.ca Fri Mar 27 10:31:09 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Fri, 27 Mar 2020 15:31:09 +0000 Subject: [Histonet] BRAF testing In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2ED459D1C3@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2ED459D1C3@HHCEXCHMB03.hhcsystem.org> Message-ID: <_qsdiMyLLGH4iaWxoWdp9uOkgg59hnVQj69GPYCOeHNKrVTOAa-HO6iGtfBqyt7IRB7gFDIHDtPZXox9xHZ7nA0X_J8UrmPm3rUTLTnW73Y=@ciqc.ca> CPQA have performed at least six B-Raf quality assurance challenges over the last few years using TMAs made of 40 tissue cores with known mutational status. Participating labs have consistently done well and their protocols are available within the reports. In addition to the results there are some photographs for reference. Go to http://cpqa.ca/assessments/Run%20101%20BRAFV600E%20Summary.pdf to see the B-Raf report from last year. Should you be interested in a clinically based EQA programme for IHC or just wish to know more about our organisation go to www.cpqa.ca Also, I do have a detailed power point on B-Raf validation, which includes other tissue types and cell lines. Please contact me directly for that if interested. Since we have validated cell lines you may wish to consider using them for working up the antibody. Go to http://www.histocyte.com/ and https://www.statlab.com/truq for cell line information. John.garratt at cpqa.ca ??????? Original Message ??????? On Friday, March 27, 2020 7:05 AM, Cartun, Richard via Histonet wrote: > This is a very difficult target to detect. I was told recently by a vendor that one needs to use prolonged, very aggressive antigen retrieval (pressure cooker with high pH buffer) to get consistent results. Also, the type of cancer may influence your success (colon vs. thyroid vs. melanoma). I would be interested in knowing what others' experiences are. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 (Office) > (860) 545-2204 (Fax) > Richard.cartun at hhchealth.org > > -----Original Message----- > From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Friday, March 27, 2020 8:07 AM > To: Histo List > Subject: [Histonet] BRAF testing > > This is an email from an external source. USE CAUTION opening attachments or links from unknown senders. > > Can anyone send me a block that can be used to validate BRAF V600E antibody. I have tried several cases that should work but our pathologists don't think it is working correctly. I just need a small core sample that is known to be positive. Any help would greatly be appreciated > > ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/http://lists.utsouthwestern.edu/mailman/listinfo/histonet;!!KCs9X-8!Oq9k97gGgRatvdlIoqX-QkrcMRMS19Iuxle4pZY1FqKfIdEBOVk2YOJ3yVO0qAn3MBg$ > > Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Mon Mar 30 17:33:00 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 30 Mar 2020 22:33:00 +0000 Subject: [Histonet] EM as a "contract lab" lab for clia purposes Message-ID: Here's a question that came up. A stand-alone EM lab at a college that is associated with a medical center, but in a different city, wants to do the EM workup for the medical center. But they would only take in samples, do the EM processing and take images then the pathologist would read the images in the other city. Does that lab need a CLIA certificate? Can they come under the existing hospital lab certificate (interpretation and signout is all done at the medical center in the other city) and be audited as contract lab? My guess is they still need a certificate since they are handling human samples for diagnostics. But Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From paula at excaliburpathology.com Mon Mar 30 18:30:49 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Mon, 30 Mar 2020 23:30:49 +0000 (UTC) Subject: [Histonet] EM as a "contract lab" lab for clia purposes In-Reply-To: References: Message-ID: <215235278.932564.1585611049226@mail.yahoo.com> Yes, they would still need the certification, however as only the technical part. A pathologist will need to be designated the medical director and all pathologists reading the results will need to have their credentials added. The medical director will need to also sign off on any complex procedures, ie. gross examination, done by the lab personnel. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Monday, March 30, 2020, 05:43:58 PM CDT, Morken, Timothy via Histonet wrote: Here's a question that came up. A stand-alone EM lab at a college that is associated with a medical center, but in a different city, wants to do the EM workup for the medical center. But they would only take in samples, do the EM processing and take images then the pathologist would read the images in the other city. Does that lab need a CLIA certificate? Can they come under the existing hospital lab certificate (interpretation and signout is all done at the medical center in the other city) and be audited as contract lab? My guess is they still need a certificate since they are handling human samples for diagnostics. But Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff at uropartners.com Tue Mar 31 08:33:44 2020 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 31 Mar 2020 13:33:44 +0000 Subject: [Histonet] A follow up to a blog post that you liked last week-haikus, puzzles and labs Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF129CD2D3@COLOEXCH01.uropartners.local> Thanks for reading-and all be well http://www.chicagonow.com/downsize-maybe/2020/03/hits-misses-covid-haikus/ Lester J. Raff, MD MBA FCAP Laboratory Director UroPartners LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 Telephone 708-486-0076 Fax 708-492-0203