From k.castillo at azdermgroup.com Tue Jun 2 09:25:45 2020 From: k.castillo at azdermgroup.com (Kristy Castillo) Date: Tue, 2 Jun 2020 07:25:45 -0700 Subject: [Histonet] Job opportunity Message-ID: Job opportunity in beautiful "Cool" Prescott, Arizona Full time with benefits and 401K No weekends or holidays Privately owned Dermatology Pathology Lab Looking for a Histotech experienced in Dermatology including grossing specimens Immunohistochemistry (IHC) knowledge preferred. Would consider training if necessary. Day time hours Come live in one of the best towns in Arizona! Kristy Castillo k.castillo at azdermgroup.com From plucas at biopath.org Tue Jun 2 11:25:03 2020 From: plucas at biopath.org (Paula) Date: Tue, 2 Jun 2020 09:25:03 -0700 Subject: [Histonet] IHC validations Message-ID: <008c01d638fa$5ff6fd50$1fe4f7f0$@biopath.org> Hello, I see 10 positive and 10 negative cases for CAP guidelines, but what about for CLIA? What is their guideline to validate an IHC antibody? Thanks in advance, Paula From CIngles at uwhealth.org Tue Jun 2 12:44:50 2020 From: CIngles at uwhealth.org (Ingles Claire) Date: Tue, 2 Jun 2020 17:44:50 +0000 Subject: [Histonet] IHC validations In-Reply-To: <008c01d638fa$5ff6fd50$1fe4f7f0$@biopath.org> References: <008c01d638fa$5ff6fd50$1fe4f7f0$@biopath.org> Message-ID: Paula: Usually CLIA follows CAP's lead or has less strict guidelines. Following the CAP guidelines should be good for both. Claire ________________________________ From: Paula via Histonet Sent: Tuesday, June 2, 2020 11:25 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC validations WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Hello, I see 10 positive and 10 negative cases for CAP guidelines, but what about for CLIA? What is their guideline to validate an IHC antibody? Thanks in advance, Paula _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmyers1 at aol.com Tue Jun 2 13:24:57 2020 From: jmyers1 at aol.com (Joe Myers) Date: Tue, 2 Jun 2020 14:24:57 -0400 Subject: [Histonet] IHC validations References: <589B5F2B-13A8-452D-B139-728F30744827.ref@aol.com> Message-ID: <589B5F2B-13A8-452D-B139-728F30744827@aol.com> ?Dear Ms. Lucas: Unfortunately, there is no specific CLIA standard that addresses the quantity of positive/negative specimens that should be tested during the procedure validation process. As stated in the regulation excerpt shown below (section 2), it is up to each laboratory to establish its own ?performance specifications? for procedures that ate developed ?in-house?, as nearly all IHC procedures are. 42 CFR 493.1253 Standard: Establishment and verification of performance specifications. (a) Applicability. Laboratories are not required to verify or establish performance specifications for any test system used by the laboratory before April 24, 2003. (b) (1) Verification of performance specifications. Each laboratory that introduces an unmodified, FDA-cleared or approved test system must do the following before reporting patient test results: (i) Demonstrate that it can obtain performance specifications comparable to those established by the manufacturer for the following performance characteristics: (A) Accuracy. (B) Precision. (C) Reportable range of test results for the test system. (ii) Verify that the manufacturer's reference intervals (normal values) are appropriate for the laboratory's patient population. (2) Establishment of performance specifications. Each laboratory that modifies an FDA-cleared or approved test system, or introduces a test system not subject to FDAclearance or approval (including methods developed in-house and standardized methods such as text book procedures), or uses a test system in which performance specifications are not provided by the manufacturer must, before reporting patienttest results, establish for each test system the performance specifications for the following performance characteristics, as applicable: (i) Accuracy. (ii) Precision. (iii) Analytical sensitivity. (iv) Analytical specificity to include interfering substances. (v) Reportable range of test results for the test system. (vi) Reference intervals (normal values). (vii) Any other performance characteristic required for test performance. (3) Determination of calibration and control procedures. The laboratory must determine the test system's calibration procedures and control procedures based upon the performance specifications verified or established under paragraph (b)(1) or (b)(2) of this section. I hope you find this feedback useful. I?ve prepared a document that outlines how a laboratory can comply with the standards and would be happy to share it with you; simply send me a message and I will respond accordingly. Best Wishes, Joe Myers, M.S., CT/QIHC(ASCP) ------------------------------ Message: 2 Date: Tue, 2 Jun 2020 09:25:03 -0700 From: "Paula" To: Subject: [Histonet] IHC validations Hello, I see 10 positive and 10 negative cases for CAP guidelines, but what about for CLIA? What is their guideline to validate an IHC antibody? Thanks in advance, Paula From criley at dpspa.com Tue Jun 2 16:19:02 2020 From: criley at dpspa.com (Charles Riley) Date: Tue, 2 Jun 2020 21:19:02 +0000 Subject: [Histonet] ANSI or ASTM certified scales Message-ID: I am prepping our lab for our next CAP inspection. Does anyone know a good place to buy analytical scales that meet the CAP COM.30860/70/80/90 checklist items? And does anyone think it is cheaper to buy a new scale each year or to have a certified tech come out to your location to re-certify its accuracy each year? Thanks in advance for any guidance you could provide From k.castillo at azdermgroup.com Wed Jun 3 08:01:08 2020 From: k.castillo at azdermgroup.com (Kristy Castillo) Date: Wed, 3 Jun 2020 06:01:08 -0700 Subject: [Histonet] Dako Auto Plus Message-ID: Does anyone have any old tube racks they want to get rid of? We use the Dako Auto Plus for our IHC and could use some more racks. Thank you in advance. Kristy k.castillo at azdermgroup.com From brringer at SpringfieldClinic.com Wed Jun 3 09:26:57 2020 From: brringer at SpringfieldClinic.com (Ringer, Bradley) Date: Wed, 3 Jun 2020 14:26:57 +0000 Subject: [Histonet] Levels on biopsies Message-ID: <30F02E79E90E7B46BA9D7B3FC705099F0208C55F0B@E2K10DB.springfieldclinic.com> Hi All, I'm curious to know how many levels your lab cuts on the following biopsies: prostate core, cervical, vaginal, bladder, and anus biopsies. I ask this because we are currently (and have been for years) giving our pathologists 3 levels/slides for each of these and they recently requested that we only give them one slide with two sections. Thank you in advance for any responses. -Brad Brad Ringer, HTL (ASCP) Springfield Clinic Histology Manager 1025 S. 6th Street Springfield, IL 62703 (217)528-7541 ext. 14078 From BMolinari at texasheart.org Wed Jun 3 13:39:37 2020 From: BMolinari at texasheart.org (Betsy Molinari) Date: Wed, 3 Jun 2020 18:39:37 +0000 Subject: [Histonet] stored in 80% isopropanol References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.69d9d504-2cfa-4223-9f79-200c4561ace0@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.cee565ae-ecb0-4aba-831d-a1b9f4c03822@emailsignatures365.codetwo.com> Message-ID: Hi, If tissue is fixed in formalin ( I do not know if it is 10% NBF) then put in 80 % isopropanol can the tissue be processed as if it was in ETOH? What are the effects to the tissue stored in isopropanol? Thanks, Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From BMolinari at texasheart.org Wed Jun 3 13:49:16 2020 From: BMolinari at texasheart.org (Betsy Molinari) Date: Wed, 3 Jun 2020 18:49:16 +0000 Subject: [Histonet] stored in 80% isopropanol In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.69d9d504-2cfa-4223-9f79-200c4561ace0@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.cee565ae-ecb0-4aba-831d-a1b9f4c03822@emailsignatures365.codetwo.com>, Message-ID: I am also in research. I have very little to go on, but I presume it is not going to yield good results. Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter From: Porter, Amy Sent: Wednesday, June 3, 2020 1:47 PM To: Betsy Molinari Subject: Re: stored in 80% isopropanol *** Important *** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Rubbing alcohol - potentially really dehydrated already depending on how long??? I am research so no recommendation for clinical..... ________________________________ From: Betsy Molinari via Histonet > Sent: Wednesday, June 3, 2020 2:39 PM To: 'Histonet at lists.utsouthwestern.edu' > Subject: [Histonet] stored in 80% isopropanol Hi, If tissue is fixed in formalin ( I do not know if it is 10% NBF) then put in 80 % isopropanol can the tissue be processed as if it was in ETOH? What are the effects to the tissue stored in isopropanol? Thanks, Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org > | facebook > | twitter > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!g0oZkH9IjlZnl595iDir-L42QNjnDXR4fAz-6J8E2Nnlq46NtE0EQZYYGhZaig$ From BMolinari at texasheart.org Wed Jun 3 13:54:52 2020 From: BMolinari at texasheart.org (Betsy Molinari) Date: Wed, 3 Jun 2020 18:54:52 +0000 Subject: [Histonet] stored in 80% isopropanol In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.69d9d504-2cfa-4223-9f79-200c4561ace0@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.cee565ae-ecb0-4aba-831d-a1b9f4c03822@emailsignatures365.codetwo.com>, Message-ID: Thank you. I am waiting to get more information on the type of tissue, how long has it been in the alcohol etc... I have just never heard of tissue being stored in isopropanol. Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter -----Original Message----- From: Whitaker, Bonnie Sent: Wednesday, June 3, 2020 1:52 PM To: Betsy Molinari Subject: RE: stored in 80% isopropanol *** Important *** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Hi Betsy, I don't think it will be THAT bad. I would proceed as normal. Good luck! Bonnie Whitaker AP Operations Director 614.293.8418 -----Original Message----- From: Betsy Molinari via Histonet Sent: Wednesday, June 3, 2020 2:49 PM To: Porter, Amy ; 'Histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] stored in 80% isopropanol CAUTION: External Email I am also in research. I have very little to go on, but I presume it is not going to yield good results. Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org https://urldefense.proofpoint.com/v2/url?u=http-3A__texasheart.org&d=DwICAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=eJv4g8Iwos-eWZLS8tW0JKLm4RiP7wEVJMWf7v2rxpg&s=iItlfpQ62N9PxxdByB3prn57PIFj6WlH4J3CSBHamqM&e= | facebook | twitter From: Porter, Amy Sent: Wednesday, June 3, 2020 1:47 PM To: Betsy Molinari Subject: Re: stored in 80% isopropanol *** Important *** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Rubbing alcohol - potentially really dehydrated already depending on how long??? I am research so no recommendation for clinical..... ________________________________ From: Betsy Molinari via Histonet > Sent: Wednesday, June 3, 2020 2:39 PM To: 'Histonet at lists.utsouthwestern.edu' > Subject: [Histonet] stored in 80% isopropanol Hi, If tissue is fixed in formalin ( I do not know if it is 10% NBF) then put in 80 % isopropanol can the tissue be processed as if it was in ETOH? What are the effects to the tissue stored in isopropanol? Thanks, Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org https://urldefense.proofpoint.com/v2/url?u=http-3A__texasheart.org&d=DwICAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=eJv4g8Iwos-eWZLS8tW0JKLm4RiP7wEVJMWf7v2rxpg&s=iItlfpQ62N9PxxdByB3prn57PIFj6WlH4J3CSBHamqM&e= > | facebook > | twitter > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!g0oZkH9IjlZnl595iDir-L42QNjnDXR4fAz-6J8E2Nnlq46NtE0EQZYYGhZaig$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFEkBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=eJv4g8Iwos-eWZLS8tW0JKLm4RiP7wEVJMWf7v2rxpg&s=OC2TQMYrIXpkwBhDQPQFnuhwIhffQEwDVUCLTIpt00o&e= From Tracy.Pheneger at Colorado.EDU Wed Jun 3 14:00:22 2020 From: Tracy.Pheneger at Colorado.EDU (Tracy Pheneger) Date: Wed, 3 Jun 2020 19:00:22 +0000 Subject: [Histonet] stored in 80% isopropanol In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.69d9d504-2cfa-4223-9f79-200c4561ace0@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.cee565ae-ecb0-4aba-831d-a1b9f4c03822@emailsignatures365.codetwo.com>, , Message-ID: Isopropanol processing is routinely used with formalin fixation. Isopropanol is miscible in paraffin, so you can skip the clearing agents. Just run it down to 100% Isopropanol as you would do on a Ethanol processing schedule, then straight into paraffin. Check this out: https://pubmed.ncbi.nlm.nih.gov/14534624/ ? Tracy Tracy Pheneger, HT/HTL (ASCP), QIHC (ASCP), LATg (AALAS) Senior Professional Research Assistant BioServe Space Technologies University of Colorado c/o Kolicko AES Univ of Colorado Rm 224, 3775 Discovery Dr Boulder CO 80309 From: Betsy Molinari via Histonet Sent: Wednesday, June 3, 2020 12:50 PM To: Porter, Amy; 'Histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] stored in 80% isopropanol I am also in research. I have very little to go on, but I presume it is not going to yield good results. Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter From: Porter, Amy Sent: Wednesday, June 3, 2020 1:47 PM To: Betsy Molinari Subject: Re: stored in 80% isopropanol *** Important *** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Rubbing alcohol - potentially really dehydrated already depending on how long??? I am research so no recommendation for clinical..... ________________________________ From: Betsy Molinari via Histonet > Sent: Wednesday, June 3, 2020 2:39 PM To: 'Histonet at lists.utsouthwestern.edu' > Subject: [Histonet] stored in 80% isopropanol Hi, If tissue is fixed in formalin ( I do not know if it is 10% NBF) then put in 80 % isopropanol can the tissue be processed as if it was in ETOH? What are the effects to the tissue stored in isopropanol? Thanks, Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org > | facebook > | twitter > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!g0oZkH9IjlZnl595iDir-L42QNjnDXR4fAz-6J8E2Nnlq46NtE0EQZYYGhZaig$> _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Wed Jun 3 14:11:24 2020 From: rsrichmond at gmail.com (Bob Richmond) Date: Wed, 3 Jun 2020 15:11:24 -0400 Subject: [Histonet] Levels on biopsies In-Reply-To: References: Message-ID: > > Brad Ringer, HTL (ASCP). histology manager at Springfield Clinic in > Springfield IL asks: > >>I'm curious to know how many levels your lab cuts on the following > biopsies: prostate core, cervical, vaginal, bladder, and anus biopsies. I > ask this because we are currently (and have been for years) giving our > pathologists 3 levels/slides for each of these and they recently requested > that we only give them one slide with two sections. Thank you in advance > for any responses.<< > Speaking only about transrectal needle core biopsies of the prostate: It's customary to cut five sections. Stain 1,3, and 5, and reserve 2 and 4 for possible immunostains. If you don't do that, your immunostains will only be successful about half the time. I've worked in a number of labs that saw numbers of prostate specimens, including a specialty prostate lab, and this is the way it was done. Your pathologists should not stint on this. Bob Richmond Samurai Pathologist Maryville TN From BMolinari at texasheart.org Wed Jun 3 14:15:54 2020 From: BMolinari at texasheart.org (Betsy Molinari) Date: Wed, 3 Jun 2020 19:15:54 +0000 Subject: [Histonet] stored in 80% isopropanol In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.69d9d504-2cfa-4223-9f79-200c4561ace0@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.cee565ae-ecb0-4aba-831d-a1b9f4c03822@emailsignatures365.codetwo.com>, Message-ID: Great. Thanks Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter -----Original Message----- From: Shirley A. Powell Sent: Wednesday, June 3, 2020 2:05 PM To: Betsy Molinari Subject: RE: stored in 80% isopropanol *** Important *** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ If you stored it in isopropyl for a long time I would go down to 70. But it may not make that much difference. Shirley -----Original Message----- From: Betsy Molinari via Histonet Sent: Wednesday, June 3, 2020 2:55 PM To: Whitaker, Bonnie ; 'Histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] stored in 80% isopropanol Thank you. I am waiting to get more information on the type of tissue, how long has it been in the alcohol etc... I have just never heard of tissue being stored in isopropanol. Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter -----Original Message----- From: Whitaker, Bonnie Sent: Wednesday, June 3, 2020 1:52 PM To: Betsy Molinari Subject: RE: stored in 80% isopropanol *** Important *** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Hi Betsy, I don't think it will be THAT bad. I would proceed as normal. Good luck! Bonnie Whitaker AP Operations Director 614.293.8418 -----Original Message----- From: Betsy Molinari via Histonet Sent: Wednesday, June 3, 2020 2:49 PM To: Porter, Amy ; 'Histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] stored in 80% isopropanol CAUTION: External Email I am also in research. I have very little to go on, but I presume it is not going to yield good results. Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org https://urldefense.proofpoint.com/v2/url?u=https-3A__nam04.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.proofpoint.com-252Fv2-252Furl-253Fu-253Dhttp-2D3A-5F-5Ftexasheart.org-2526d-253DDwICAg-2526c-253Dk9MF1d71ITtkuJx-2DPdWme51dKbmfPEvxwt8SFEkBfs4-2526r-253DBgjUe6oLZB0OAcW6Y6Rn-2Dn0Q03Ac4dWC2x8Sg24AeiY-2526m-253DeJv4g8Iwos-2DeWZLS8tW0JKLm4RiP7wEVJMWf7v2rxpg-2526s-253DiItlfpQ62N9PxxdByB3prn57PIFj6WlH4J3CSBHamqM-2526e-253D-26amp-3Bdata-3D02-257C01-257Cpowell-5Fsa-2540mercer.edu-257C52d934a54d63480a2b8408d807efcf0c-257C4fb34d2889b247109bcc30824d17fc30-257C0-257C0-257C637268073856472712-26amp-3Bsdata-3DfPzEytcyfCP2NISn6Fr1VjG9pTmCSx1XVWJS44E0PKk-253D-26amp-3Breserved-3D0&d=DwIFAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=S_0pz3SElku7XDmfmfetEou6sqBc-7VXKt-24NnYL1U&s=0Mm1Am61kq_EOuoPTCi0A6fQNBvjFy2brGpOLpbdgv8&e= | facebook | twitter From: Porter, Amy Sent: Wednesday, June 3, 2020 1:47 PM To: Betsy Molinari Subject: Re: stored in 80% isopropanol *** Important *** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Rubbing alcohol - potentially really dehydrated already depending on how long??? I am research so no recommendation for clinical..... ________________________________ From: Betsy Molinari via Histonet > Sent: Wednesday, June 3, 2020 2:39 PM To: 'Histonet at lists.utsouthwestern.edu' > Subject: [Histonet] stored in 80% isopropanol Hi, If tissue is fixed in formalin ( I do not know if it is 10% NBF) then put in 80 % isopropanol can the tissue be processed as if it was in ETOH? What are the effects to the tissue stored in isopropanol? Thanks, Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org https://urldefense.proofpoint.com/v2/url?u=https-3A__nam04.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.proofpoint.com-252Fv2-252Furl-253Fu-253Dhttp-2D3A-5F-5Ftexasheart.org-2526d-253DDwICAg-2526c-253Dk9MF1d71ITtkuJx-2DPdWme51dKbmfPEvxwt8SFEkBfs4-2526r-253DBgjUe6oLZB0OAcW6Y6Rn-2Dn0Q03Ac4dWC2x8Sg24AeiY-2526m-253DeJv4g8Iwos-2DeWZLS8tW0JKLm4RiP7wEVJMWf7v2rxpg-2526s-253DiItlfpQ62N9PxxdByB3prn57PIFj6WlH4J3CSBHamqM-2526e-253D-26amp-3Bdata-3D02-257C01-257Cpowell-5Fsa-2540mercer.edu-257C52d934a54d63480a2b8408d807efcf0c-257C4fb34d2889b247109bcc30824d17fc30-257C0-257C0-257C637268073856472712-26amp-3Bsdata-3DfPzEytcyfCP2NISn6Fr1VjG9pTmCSx1XVWJS44E0PKk-253D-26amp-3Breserved-3D0&d=DwIFAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=S_0pz3SElku7XDmfmfetEou6sqBc-7VXKt-24NnYL1U&s=0Mm1Am61kq_EOuoPTCi0A6fQNBvjFy2brGpOLpbdgv8&e= > | facebook > | twitter > This email and attachments contain information that may be confidential or privileged. 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Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter From: Tracy Pheneger Sent: Wednesday, June 3, 2020 2:00 PM To: Betsy Molinari ; Porter, Amy ; 'Histonet at lists.utsouthwestern.edu' Subject: RE: stored in 80% isopropanol *** Important *** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Isopropanol processing is routinely used with formalin fixation. Isopropanol is miscible in paraffin, so you can skip the clearing agents. Just run it down to 100% Isopropanol as you would do on a Ethanol processing schedule, then straight into paraffin. Check this out: https://pubmed.ncbi.nlm.nih.gov/14534624/ 😊 Tracy Tracy Pheneger, HT/HTL (ASCP), QIHC (ASCP), LATg (AALAS) Senior Professional Research Assistant BioServe Space Technologies University of Colorado c/o Kolicko AES Univ of Colorado Rm 224, 3775 Discovery Dr Boulder CO 80309 From: Betsy Molinari via Histonet Sent: Wednesday, June 3, 2020 12:50 PM To: Porter, Amy; 'Histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] stored in 80% isopropanol I am also in research. I have very little to go on, but I presume it is not going to yield good results. Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook> | twitter> From: Porter, Amy > Sent: Wednesday, June 3, 2020 1:47 PM To: Betsy Molinari > Subject: Re: stored in 80% isopropanol *** Important *** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Rubbing alcohol - potentially really dehydrated already depending on how long??? I am research so no recommendation for clinical..... ________________________________ From: Betsy Molinari via Histonet > | facebook > | twitter > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!g0oZkH9IjlZnl595iDir-L42QNjnDXR4fAz-6J8E2Nnlq46NtE0EQZYYGhZaig$> _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at dpspa.com Wed Jun 3 14:50:19 2020 From: criley at dpspa.com (Charles Riley) Date: Wed, 3 Jun 2020 19:50:19 +0000 Subject: [Histonet] Levels on biopsies In-Reply-To: <30F02E79E90E7B46BA9D7B3FC705099F0208C55F0B@E2K10DB.springfieldclinic.com> References: <30F02E79E90E7B46BA9D7B3FC705099F0208C55F0B@E2K10DB.springfieldclinic.com> Message-ID: We usually cut 3 lvls/slides for prostate/vaginal/bladder and anus. 6 lvls/slides for cervical tissues. In the end it is the pathologists comfort level with sectioning to make the diagnosis. ________________________________ From: Ringer, Bradley via Histonet Sent: Wednesday, June 3, 2020 10:26 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Levels on biopsies Hi All, I'm curious to know how many levels your lab cuts on the following biopsies: prostate core, cervical, vaginal, bladder, and anus biopsies. I ask this because we are currently (and have been for years) giving our pathologists 3 levels/slides for each of these and they recently requested that we only give them one slide with two sections. Thank you in advance for any responses. -Brad Brad Ringer, HTL (ASCP) Springfield Clinic Histology Manager 1025 S. 6th Street Springfield, IL 62703 (217)528-7541 ext. 14078 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Donna.Willis at BSWHealth.org Wed Jun 3 17:43:26 2020 From: Donna.Willis at BSWHealth.org (Willis, Donna G) Date: Wed, 3 Jun 2020 22:43:26 +0000 Subject: [Histonet] stored in 80% isopropanol In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.69d9d504-2cfa-4223-9f79-200c4561ace0@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.cee565ae-ecb0-4aba-831d-a1b9f4c03822@emailsignatures365.codetwo.com>, Message-ID: 80% Isopropanol has 20% water. You will still have water in the tissue. Donna Willis Anatomic Pathology Manager Baylor University Medical Center Baylor Scott&White Health 214-820-2465 office ________________________________ From: Betsy Molinari via Histonet Sent: Wednesday, June 3, 2020 1:39:37 PM To: 'Histonet at lists.utsouthwestern.edu' Subject: {EXTERNAL} [Histonet] stored in 80% isopropanol CAUTION: This email originated outside of BSWH; avoid action unless you know the content is safe. Send suspicious emails as attachments to BadEmail at BSWHealth.org. Hi, If tissue is fixed in formalin ( I do not know if it is 10% NBF) then put in 80 % isopropanol can the tissue be processed as if it was in ETOH? What are the effects to the tissue stored in isopropanol? Thanks, Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!WzVzLwUdHwnEwfi21HycMVIR_Opvs33FnbnA3ypVvmqfCeTUg_D6vYwIHkKyJiyP5n-E7Q$ ********************************************************************** The information contained in this e-mail may be privileged and/or confidential, and protected from disclosure, and no waiver of any attorney-client, work product, or other privilege is intended. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. The sender and Baylor Scott & White Health, and its affiliated entities, hereby expressly reserve all privileges and confidentiality that might otherwise be waived as a result of an erroneous or misdirected e-mail transmission. No employee or agent is authorized to conclude any binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, by e-mail without express written confirmation by the CEO, the Senior Vice President of Supply Chain Services or other duly authorized representative of Baylor Scott & White Health. From BMolinari at texasheart.org Thu Jun 4 06:35:47 2020 From: BMolinari at texasheart.org (Betsy Molinari) Date: Thu, 4 Jun 2020 11:35:47 +0000 Subject: [Histonet] stored in 80% isopropanol In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.69d9d504-2cfa-4223-9f79-200c4561ace0@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.cee565ae-ecb0-4aba-831d-a1b9f4c03822@emailsignatures365.codetwo.com>, Message-ID: Yes, but I plan to put them in my processor starting at 95% ETOH and continue with my regular processing. I am assuming there should be no issues with that correct? Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter From: Willis, Donna G Sent: Wednesday, June 3, 2020 5:43 PM To: Betsy Molinari ; 'Histonet at lists.utsouthwestern.edu' Subject: Re: stored in 80% isopropanol *** Important *** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ 80% Isopropanol has 20% water. You will still have water in the tissue. Donna Willis Anatomic Pathology Manager Baylor University Medical Center Baylor Scott&White Health 214-820-2465 office ________________________________ From: Betsy Molinari via Histonet > Sent: Wednesday, June 3, 2020 1:39:37 PM To: 'Histonet at lists.utsouthwestern.edu' > Subject: {EXTERNAL} [Histonet] stored in 80% isopropanol CAUTION: This email originated outside of BSWH; avoid action unless you know the content is safe. Send suspicious emails as attachments to BadEmail at BSWHealth.org. Hi, If tissue is fixed in formalin ( I do not know if it is 10% NBF) then put in 80 % isopropanol can the tissue be processed as if it was in ETOH? What are the effects to the tissue stored in isopropanol? Thanks, Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org > | facebook > | twitter > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!WzVzLwUdHwnEwfi21HycMVIR_Opvs33FnbnA3ypVvmqfCeTUg_D6vYwIHkKyJiyP5n-E7Q$ ________________________________ The information contained in this e-mail may be privileged and/or confidential, and protected from disclosure, and no waiver of any attorney-client, work product, or other privilege is intended. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. The sender and Baylor Scott & White Health, and its affiliated entities, hereby expressly reserve all privileges and confidentiality that might otherwise be waived as a result of an erroneous or misdirected e-mail transmission. No employee or agent is authorized to conclude any binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, by e-mail without express written confirmation by the CEO, the Senior Vice President of Supply Chain Services or other duly authorized representative of Baylor Scott & White Health. From BMolinari at texasheart.org Thu Jun 4 06:37:04 2020 From: BMolinari at texasheart.org (Betsy Molinari) Date: Thu, 4 Jun 2020 11:37:04 +0000 Subject: [Histonet] stored in 80% isopropanol In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.69d9d504-2cfa-4223-9f79-200c4561ace0@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.cee565ae-ecb0-4aba-831d-a1b9f4c03822@emailsignatures365.codetwo.com>, , Message-ID: That is very interesting. Thank you for mentioning that! Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter -----Original Message----- From: Gilchrist, Eric P Sent: Wednesday, June 3, 2020 3:14 PM To: Tracy Pheneger ; Betsy Molinari ; Porter, Amy Subject: RE: stored in 80% isopropanol *** Important *** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ If I am not mistaken, Thermo-Fisher's "Reagent Alcohols" are all Isopropanol based. When we have providers who do not have a biopsy kit available and have a specimen I usually advise them to put the tissue in 70% isopropanol to fix and store and then overnight a biopsy kit to them. Eric Gilchrist | Supervisor Oral & Maxillofacial Pathology University of North Carolina Adams School of Dentistry P 919.537.3153 | F 919.843.6508 -----Original Message----- From: Tracy Pheneger via Histonet Sent: Wednesday, June 3, 2020 3:00 PM To: Betsy Molinari ; Porter, Amy ; 'Histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] stored in 80% isopropanol Isopropanol processing is routinely used with formalin fixation. Isopropanol is miscible in paraffin, so you can skip the clearing agents. Just run it down to 100% Isopropanol as you would do on a Ethanol processing schedule, then straight into paraffin. Check this out: https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_14534624_&d=DwIGaQ&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=Z8lCQROWymUZDTR2-MB-L6oGzMGD3l5tsvfWpQLIcGs&s=yJZyBp1_0VSLcTPQXOs29ban8nT7qsaQzMbA9StpsRA&e= ? Tracy Tracy Pheneger, HT/HTL (ASCP), QIHC (ASCP), LATg (AALAS) Senior Professional Research Assistant BioServe Space Technologies University of Colorado c/o Kolicko AES Univ of Colorado Rm 224, 3775 Discovery Dr Boulder CO 80309 From: Betsy Molinari via Histonet Sent: Wednesday, June 3, 2020 12:50 PM To: Porter, Amy; 'Histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] stored in 80% isopropanol I am also in research. I have very little to go on, but I presume it is not going to yield good results. Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter From: Porter, Amy Sent: Wednesday, June 3, 2020 1:47 PM To: Betsy Molinari Subject: Re: stored in 80% isopropanol *** Important *** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Rubbing alcohol - potentially really dehydrated already depending on how long??? I am research so no recommendation for clinical..... ________________________________ From: Betsy Molinari via Histonet > Sent: Wednesday, June 3, 2020 2:39 PM To: 'Histonet at lists.utsouthwestern.edu' > Subject: [Histonet] stored in 80% isopropanol Hi, If tissue is fixed in formalin ( I do not know if it is 10% NBF) then put in 80 % isopropanol can the tissue be processed as if it was in ETOH? What are the effects to the tissue stored in isopropanol? Thanks, Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org > | facebook > | twitter > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!g0oZkH9IjlZnl595iDir-L42QNjnDXR4fAz-6J8E2Nnlq46NtE0EQZYYGhZaig$> _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=Z8lCQROWymUZDTR2-MB-L6oGzMGD3l5tsvfWpQLIcGs&s=h_nMob8V8lpZ8yQVrgNN1ShHdD4CBYTaOTtGdGUnoMQ&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=Z8lCQROWymUZDTR2-MB-L6oGzMGD3l5tsvfWpQLIcGs&s=h_nMob8V8lpZ8yQVrgNN1ShHdD4CBYTaOTtGdGUnoMQ&e= From Katherine.Bummer at tevapharm.com Thu Jun 4 11:52:57 2020 From: Katherine.Bummer at tevapharm.com (Katherine Bummer) Date: Thu, 4 Jun 2020 16:52:57 +0000 Subject: [Histonet] Leica Bond Rx covertile cleaning question? Message-ID: Hello Histonetters, I wanted to find out how you are cleaning your covertiles that are used for chromogenic & Immunofluourescent IHC as well as RNAscope? Thank you! Kate This message is intended solely for the designated recipient(s). It may contain confidential or proprietary information and may be subject to attorney-client privilege or other confidentiality protections. If you are not a designated recipient you may not review, copy or distribute this message. If you receive this in error, please notify the sender by reply e-mail and delete this message. Thank you. From carl.hobbs at kcl.ac.uk Thu Jun 4 12:59:20 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Thu, 4 Jun 2020 17:59:20 +0000 Subject: [Histonet] stored in 80% isopropanol Message-ID: If you have a processer using IMS ( or the more expensive ethanol) take tissues from that 80% IPA ( iso Propyl alcohol) to a fresh 80% IMS/ethanol, whichever you use as std and continue as normal ( you have no idea if the specimens were taken from water/PFA directly into 80% IPA? Thus there will be more than 20% water in there....) IMS/IPA/EtOH are all compatible for Pwax processing, where a std clearing agent is used ( eg: Xylene, Histoclear) Sure, you can as suggested use increasing concs IPA and go directly into wax, bypassing the "clearing" stage but, imho longer Pwax times are needed as IPA is not miscible in Pwax unless at the high temp used.....no big deal. I have used this method in the past, with success Tho I have to admit that I still for my sins, find that IMS then Xylene gives the best final Pwax block! If others disagree, I'd be grateful to know what their safer equally as good clearing agent alternatives are and the cost/block compared with using xylene. Cost/benefit? Best wishes Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From relia1 at earthlink.net Wed Jun 10 10:30:18 2020 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 10 Jun 2020 11:30:18 -0400 Subject: [Histonet] Can I help? Message-ID: <000001d63f3c$0c4c4140$24e4c3c0$@earthlink.net> Hi Histopeeps, How are you? I have been hearing from many of our fellow histopeeps who have asked for help with resume tune ups and job hunting/ interview advice. So I thought I would reach out and see if there is anything I can do for you: ? A Resume Tune-up ? Help with Salary Negotiation ? Interview Advice In case you missed it-Here is a link to a great article from ?Ask a Manager? If you are furloughed how long should you wait to look for a new job? https://www.askamanager.org/2020/05/if-youre-furloughed-how-long-should-you- wait-to-look-for-a-new-job.html Histopeeps, What?s happening on your end? ? Have you been called back to work ? full time or part time? ? Unemployed? Laid off? Furloughed? ? Are you a traveler that wants to get back to permanent work? ? Are you a histotech that wants to change the type of histology you do? ? Do you need a resume tune up? ? Do you need some career advice? I am here if you need me and would love to know what is happening on your end. If you have a minute shoot me back an email and let me know what?s up and I promise to share with you what I am seeing in other places. Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From andrea at ka-recruiting.com Wed Jun 10 12:21:55 2020 From: andrea at ka-recruiting.com (Andrea Costello) Date: Wed, 10 Jun 2020 13:21:55 -0400 Subject: [Histonet] Good News! Surge in Hiring for Histology Positions Message-ID: Hello Histonetters - I hope you are all doing well. I have seen first hand that over the past few months there has been an increasing number of furloughs, layoffs or reduction of hours across the histology field. However, some great news! - I have noticed over the past two weeks a large influx of laboratories that are seeking *permanent and full time *histotechs from the bench to management positions! I have included a list of these new openings below - if you are interested please feel free to contact *andrea at ka-recruiting.com *with an updated resume and the best time & phone number for me to reach you. Florida - Histotech (Evening Shift) & Histology Manager Georgia - Histotech (Multiple Shifts Available) North Dakota - Histotech & Lead Histotech (Multiple Shifts Available) New York - Histology Manager South Carolina - Histotech Tennessee - Histotech (Multiple Shifts Available) Wisconsin - Lead Histotech (Evening or Night Shift) Andrea Costello Client Relationship Manager Senior Healthcare Recruiter K.A. Recruiting, Inc. *P: (617) 746-2745* *F: (617) 507-8009 (617) 507-8009 * andrea at ka-recruiting.com From mike_s_pence at msn.com Thu Jun 11 11:05:57 2020 From: mike_s_pence at msn.com (MICHAEL S KATHY PENCE) Date: Thu, 11 Jun 2020 16:05:57 +0000 Subject: [Histonet] Can't keep a good histo tech down Message-ID: Yesterday I was called into my director's office and informed by the section VP that they were eliminating my job of 35 years as Supervisor of Anatomic Pathology. So here I am on Histonet seeing if anyone out there is looking for a VERY skilled grossing HT for temp services. If you are feel free to contact me and we can talk. Sent from my U.S.Cellular? Smartphone From jaylundgren at gmail.com Thu Jun 11 12:22:30 2020 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 11 Jun 2020 12:22:30 -0500 Subject: [Histonet] Can't keep a good histo tech down In-Reply-To: References: Message-ID: If you want to be a temp, be a temp. There are literally dozens of agencies you can join. Histonet is not a job board. From relia1 at earthlink.net Fri Jun 12 11:03:08 2020 From: relia1 at earthlink.net (relia1) Date: Fri, 12 Jun 2020 12:03:08 -0400 Subject: [Histonet] FW: Details for MaSH webinar series In-Reply-To: Message-ID: Posting for a friend ?Thanks-PamRight place Right time Right move with RELIA!Thank youPam Barker?RELIA Solutions?Cell 407-353-5070relia1 at earthlink.net?#jobs4myhistopeeps?#ilovemyhistopeeps?Sent from my Sprint Samsung Galaxy S10+. -------- Original message --------From: Gina Benedetto Date: 6/9/20 8:14 AM (GMT-05:00) To: relia1 at earthlink.net Subject: Details for MaSH webinar series Hello Pam,?Thank you so much for your LinkedIn message! Here are the details for our webinar series coming this Monday:?Coming the week of June 15th - 20th, the Massachusetts Society of Histotechnology will be hosting a virtual Webinar Series! Each day features a different speaker who will discuss various engaging topics, ranging from multiplex immunofluorescence and next generation sequencing to inspirational leadership strategies. Join us for this chance to earn CEUs and learn something unique each day, as well as the chance to win a $50 Amazon giftcard! Visit our website for registration and additional information: https://www.mahistology.org/?Thank you so much for your help in spreading the word about this opportunity :)?Best wishes,?Gina??-- Gina Benedetto Associate Scientist II | Translational Neuropathology Biogen | 115 Broadway Cambridge, MA 02142 Cell: (781) 635-4193?| Work: (617) 914-7707 x47707 LinkedIn:?https://www.linkedin.com/in/ginabenedetto/ From PKRichar at gundersenhealth.org Mon Jun 15 15:19:31 2020 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Mon, 15 Jun 2020 20:19:31 +0000 Subject: [Histonet] COVID 19 Policy Message-ID: Hi, has anyone created a policy that you are willing to share for the handling of fresh specimens from suspect or COVID positive patients? Pam ~ National Histology Professionals Day 3/10/20 Pathologists' Assistant Day 4/14/2020 Medical Laboratory Professionals Week April 19-25, 2020 National Cytotechnology Day 5/13/2020 +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org From madeleinehuey at gmail.com Tue Jun 16 13:29:39 2020 From: madeleinehuey at gmail.com (Madeleine Huey) Date: Tue, 16 Jun 2020 11:29:39 -0700 Subject: [Histonet] Histonet Digest, Vol 199, Issue 9 In-Reply-To: References: Message-ID: Hello, Does anyone have Covid 19 FFPE tissues or Transient/Stable Cell Line (slides/block) ? I have some H.Pylori blocks for exchanges. *Madeleine Huey, B.S., HTL & QIHC (ASCP)* Supervisor, Pathology/Histology & IPOX (MV & LG) El Camino Hospital 2500 Grant Road (Rm GC-33) Mountain View, CA 94040 Phone: 650-940-7038 Fax: 650-988-8387 Email: madeleine_h at elcaminohospital.org On Tue, Jun 16, 2020 at 10:20 AM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. COVID 19 Policy (Richardson, Pam K) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 15 Jun 2020 20:19:31 +0000 > From: "Richardson, Pam K" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] COVID 19 Policy > Message-ID: > < > BYAPR10MB25820BE19A2D0ED3386B37C6DD9C0 at BYAPR10MB2582.namprd10.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > Hi, has anyone created a policy that you are willing to share for the > handling of fresh specimens from suspect or COVID positive patients? > > Pam ~ > National Histology Professionals Day 3/10/20 > Pathologists' Assistant Day 4/14/2020 > Medical Laboratory Professionals Week April 19-25, 2020 > National Cytotechnology Day 5/13/2020 > > +++++++++++++++++++++++++++ > Pam Richardson > Clinical Manager > Gundersen Health System Laboratory Services > Email: pkrichar at gundersenhealth.org > Phone: 608 775-4133 > Fax: 608 775-6136 > Interdepartmental Mail Stop: H04-007 > E-visit us at: http://www.gundersenhealth.org > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 199, Issue 9 > **************************************** > From kdean70 at hotmail.com Tue Jun 16 14:50:48 2020 From: kdean70 at hotmail.com (Ken M) Date: Tue, 16 Jun 2020 19:50:48 +0000 Subject: [Histonet] Anyone having difficulty with tissue adhesion? Message-ID: Hi everyone We have developed a new hydrophilic IHC microscope slide that uses a new adhesive process that dries up to 50% faster than a normal Silane coated charged slide and we are looking for someone that might be using a Ventana or who may be dealing with difficult tissue or a difficult protocol that may be causing them to lose tissue who can try them for us in a difficult real world setting. If Anyone is interested, I can send you several packs at no charge. Ken Kdean70 at hotmail.com From criley at dpspa.com Tue Jun 16 15:25:02 2020 From: criley at dpspa.com (Charles Riley) Date: Tue, 16 Jun 2020 20:25:02 +0000 Subject: [Histonet] Anyone having difficulty with tissue adhesion? In-Reply-To: References: Message-ID: My lab is having constant issue with tissues falling off. We use Leica Bonds but certain tissues continuously give us issues. Our address is 1253 college Park Drive, Dover DE 19904 I would love to test out your slides and give feedback ________________________________ From: Ken M via Histonet Sent: Tuesday, June 16, 2020 3:50 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Anyone having difficulty with tissue adhesion? Hi everyone We have developed a new hydrophilic IHC microscope slide that uses a new adhesive process that dries up to 50% faster than a normal Silane coated charged slide and we are looking for someone that might be using a Ventana or who may be dealing with difficult tissue or a difficult protocol that may be causing them to lose tissue who can try them for us in a difficult real world setting. If Anyone is interested, I can send you several packs at no charge. Ken Kdean70 at hotmail.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paula at excaliburpathology.com Tue Jun 16 15:48:19 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Tue, 16 Jun 2020 20:48:19 +0000 (UTC) Subject: [Histonet] Anyone having difficulty with tissue adhesion? In-Reply-To: References: Message-ID: <19326197.1316913.1592340499375@mail.yahoo.com> Hello, 99% of my work are eyes and they are notorious for not adhering well. One thing I have found is that white glass slides work the best and cannot use any green glass. I would like to try your slides. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Tuesday, June 16, 2020, 03:01:41 PM CDT, Ken M via Histonet wrote: Hi everyone We have developed a new hydrophilic IHC microscope slide that uses a new adhesive process that dries up to 50% faster than a normal Silane coated charged slide and we are looking for someone that might be using a Ventana or who may be dealing with difficult tissue or a difficult protocol that may be causing them to lose tissue who can try them for us in a difficult real world setting. If Anyone is interested, I can send you several packs at no charge. Ken Kdean70 at hotmail.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Wed Jun 17 12:32:08 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Wed, 17 Jun 2020 17:32:08 +0000 Subject: [Histonet] Anyone having difficulty with tissue adhesion? Message-ID: I am very interested. I do IHC on Pwax sections of mouse bone, human keloid tissue ( got a project aching to start which hopefully will result in a publication) and mouse/rat contused spinal cord. All are quite difficult to keep on the slides as I have to, mostly, use HIER to get the best IHC- positive result. The best slides I have used to date are Trajan's Series 3. ( superior to commercial Superfrost Plus, homemade Silanated or double-subbed slides) However, they do not retain bone/cartilage...sure, keloid collagen is much better retained but there is still some loss Sure, I get researchers to buy only them for the tissues stated above. I will not be returning to work until 3rd August....maybe later. It depends...?. Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From amurvosh at advancederm.net Tue Jun 23 09:39:59 2020 From: amurvosh at advancederm.net (Anne Murvosh) Date: Tue, 23 Jun 2020 14:39:59 +0000 Subject: [Histonet] Xylene recycle Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7CB9401@Exchange.Advancederm.net> I'm looking to switch from a disposal pick-up to recycling of Xylene. Can anyone tell me some companies that offer a recycling unit. The catch is we don't have very much so I need something for a small lab. Thanks Anne From deedeebrina at aol.com Tue Jun 23 18:53:46 2020 From: deedeebrina at aol.com (Dee Neamand) Date: Tue, 23 Jun 2020 19:53:46 -0400 Subject: [Histonet] Histonet Digest, Vol 199, Issue 12 In-Reply-To: References: Message-ID: Hi Anne. We?ve been recycling xylene for many years. We purchased our unit through CBG biotech. They have a formalin recycling unit and a solvent recycling unit. The solvent unit can be used to recycle ethanol, xylene, and xylene substitutes. Let me know if you need more info or would like to chat about them. Dee Neamand, HT (ASCP) Lead Tech Evangelical Community Hospital Lewisburg PA Sent from my iPhone > On Jun 23, 2020, at 1:17 PM, histonet-request at lists.utsouthwestern.edu wrote: > > ?Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Xylene recycle (Anne Murvosh) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 23 Jun 2020 14:39:59 +0000 > From: Anne Murvosh > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Xylene recycle > Message-ID: > <22BDD9AABC13E24E95D1CF064B75C4B7CB9401 at Exchange.Advancederm.net> > Content-Type: text/plain; charset="us-ascii" > > I'm looking to switch from a disposal pick-up to recycling of Xylene. Can anyone tell me some companies that offer a recycling unit. The catch is we don't have very much so I need something for a small lab. Thanks Anne > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 199, Issue 12 > ***************************************** From edmartin26 at gmail.com Tue Jun 23 19:52:14 2020 From: edmartin26 at gmail.com (Eddie Martin) Date: Tue, 23 Jun 2020 20:52:14 -0400 Subject: [Histonet] Xylene recycle or purchase fresh In-Reply-To: <22BDD9AABC13E24E95D1CF064B75C4B7CB9401@Exchange.Advancederm.net> References: <22BDD9AABC13E24E95D1CF064B75C4B7CB9401@Exchange.Advancederm.net> Message-ID: Hi Anne, If your xylene consumption is small, the footprint needed to recycle xylene in house and associated cost would be more expensive than buying xylene and shipping out the waste. V/r, Eddie Martin Sent from my iPhone > On Jun 23, 2020, at 10:39 AM, Anne Murvosh wrote: > > ?I'm looking to switch from a disposal pick-up to recycling of Xylene. Can anyone tell me some companies that offer a recycling unit. The catch is we don't have very much so I need something for a small lab. Thanks Anne > From john.garratt at ciqc.ca Tue Jun 23 20:24:56 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Wed, 24 Jun 2020 01:24:56 +0000 Subject: [Histonet] Xylene recycle or purchase fresh In-Reply-To: References: <22BDD9AABC13E24E95D1CF064B75C4B7CB9401@Exchange.Advancederm.net> Message-ID: You may wish to consider consulting with your local fire officer to find out what requirements are for installing a recycling unit in your facility. You may not even be allowed to place one. Also, consider the fumes from recycling xylene and if you want your expensive lab labour to be taken off the bench to process xylene. John www.cpqa.ca ??????? Original Message ??????? On Tuesday, June 23, 2020 5:52 PM, Eddie Martin via Histonet wrote: > Hi Anne, > > If your xylene consumption is small, the footprint needed to recycle xylene in house and associated cost would be more expensive than buying xylene and shipping out the waste. > > V/r, > Eddie Martin > > Sent from my iPhone > > > On Jun 23, 2020, at 10:39 AM, Anne Murvosh amurvosh at advancederm.net wrote: > > I'm looking to switch from a disposal pick-up to recycling of Xylene. Can anyone tell me some companies that offer a recycling unit. The catch is we don't have very much so I need something for a small lab. Thanks Anne > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From edmartin26 at gmail.com Tue Jun 23 20:42:19 2020 From: edmartin26 at gmail.com (Eddie Martin) Date: Tue, 23 Jun 2020 21:42:19 -0400 Subject: [Histonet] Can't keep a good histo tech down In-Reply-To: References: Message-ID: Hi Mike. I?m really sorry to hear that they?re terminating your role especially since you?ve filled that role for over 35 years at the same laboratory. FYI: Pam Barker with Relia is advertising a lead Ht role in a growing Tampa Bay lab. Pam?s number is 407-353-5070 I don?t want to just say ?Best wishes in your new job search?. If you would like to talk, there are other histology contract providers I can send your way or discuss further with you. My office number will be posted below. I can schedule a break from work and discuss with you. For starters: **Do you have a resume ready to go? **Have you marketed yourself on job boards like indeed.com or monster.com? **Have you created a LinkedIn account to friend potential future jobs or job related connections that might provide an introduction to a position you would be intrigued to pursue? Or even list tasks you?re highly skilled at? **do you have a digital trail of posts you replied to that show your subject matter expertise or willingness to help others that you could bring up during interviews? **being a subject matter expert in histology is too great a skill set to keep in place. Have you considered working as a vendor for histology products or applications like IHC or selling equipment like microtomes and stainers? ***or considered going solo as a consultant or MOHS technician? Your former job may have given you a dis-service...But it?s up to you on how you can make that turn around for yourself. This could be an opportunity to expand your wings into other histology related venues if you so wish. V/r, Eddie Martin NIH/CC/DLM Hematology Service Team Leader 301-594-2054 office number Sent from my iPhone > On Jun 11, 2020, at 12:05 PM, MICHAEL S KATHY PENCE wrote: > > ?Yesterday I was called into my director's office and informed by the section VP that they were eliminating my job of 35 years as Supervisor of Anatomic Pathology. So here I am on Histonet seeing if anyone out there is looking for a VERY skilled grossing HT for temp services. If you are feel free to contact me and we can talk. > > > > Sent from my U.S.Cellular? Smartphone > From relia1 at earthlink.net Wed Jun 24 12:47:46 2020 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 24 Jun 2020 13:47:46 -0400 Subject: [Histonet] Histo Tech needed in GA!! Can you help? Message-ID: <08cb01d64a4f$92569d30$b703d790$@earthlink.net> Hi Histopeeps, How are you? I hope you are having a great week. I have an exciting opportunity in Atlanta, GA to share for you or a fellow histopeep! I have been engaged on a search by a long time Atlanta area client who is in need of a Mohs Histotech that is CLIA qualified to gross. Here is the info: We are looking for a Grossing Mohs Tech who is CLIA qualified to gross. The practice is located North of the downtown area. (Buckhead/Peachtree) This is a full time permanent position 8-5 M-Thurs and 8-1 on Friday. My client offers a competitive pay rate and excellent benefits. There is excellent job security and opportunity for advancement as this client is part of a larger nationwide dermatology group. The help I need from you Histopeeps is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them or pass their contact information to me? *remember if I place someone you refer to me you earn a referral bonus* This positions is full time and permanent. And this client is ready to interview and hire!! If you are interested in this position please contact me ASAP On my cell/text 407-353-5070 or toll free at 866-607-3542 Or via email at relia1 at earthlink.net If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won't release it to anyone without your permission. Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From kdean70 at hotmail.com Tue Jun 30 16:20:54 2020 From: kdean70 at hotmail.com (Ken M) Date: Tue, 30 Jun 2020 21:20:54 +0000 Subject: [Histonet] Gram Staining Message-ID: Hi. We are trying to stain a supposed Gram Positive paraffin Tonsil tissue block using Gentian Violet, Iodine (Lugol), Gram's decolorizer, Carbol Fuchsin Counter and Tartrazine and are having trouble distinguishing the positive from the negative. Anyone have any suggestions? From tony.henwood at health.nsw.gov.au Tue Jun 30 18:35:54 2020 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue, 30 Jun 2020 23:35:54 +0000 Subject: [Histonet] Gram Staining In-Reply-To: References: Message-ID: Check the age of the Iodine. Iodine solutions lose their activity over time (?notice the brown discolouration on the parafilm sealing a coplin jar) even though the solution still looks as brown as when it was made. Magee, C. M., Rodeheaver, G., Edgerton, M. T., & Edlich, R. F. (1975). A more reliable gram staining technic for diagnosis of surgical infections. The American Journal of Surgery, 130(3), 341-346. "Opening the bottle in a warm room potentiated the loss of iodine so rapidly that no iodine could be detected in the solutions after fifteen days. The potentiation of loss of iodine by temperature elevation becomes a severe problem in the warm seasons of the year, particularly in tropical areas." And "When iodine concentration of the solution was less than 20 per cent of the original value, some of the stained slides revealed only gram-negative organisms." Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Ken M via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 1 July 2020 7:21 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Gram Staining Hi. We are trying to stain a supposed Gram Positive paraffin Tonsil tissue block using Gentian Violet, Iodine (Lugol), Gram's decolorizer, Carbol Fuchsin Counter and Tartrazine and are having trouble distinguishing the positive from the negative. Anyone have any suggestions? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities.