From rsrichmond at gmail.com Wed Jul 1 13:27:23 2020 From: rsrichmond at gmail.com (Bob Richmond) Date: Wed, 1 Jul 2020 14:27:23 -0400 Subject: [Histonet] Gram staining In-Reply-To: References: Message-ID: > > In answer to a query about tissue Gram staining, Tony Henwood adds > instability of the iodine solution to the long list of reasons why tissue > Gram staining doesn't work very well. > The Gram stain done on smears is of course one of the most useful microtechniques in microbiology, but tissue Gram stains work much less well, and are of very limited use. Unfortunately pathologists are fixated on them, so they're very hard to ban from the laboratory. Pathologist, if you wanna see bugs, get a toluidine blue or Diff-Quik 2 or whatever blue bug stain your lab uses. Quantitating bacteria in tissue sections has its uses (in burn tissue, for example), but if you want identification, somebody shoulda got a culture. Or use a specific immunohistochemical technique for the organism you suspect. Bob Richmond Samurai Pathologist Maryville TN From megaphred91 at gmail.com Thu Jul 2 11:49:18 2020 From: megaphred91 at gmail.com (fred fred) Date: Thu, 2 Jul 2020 12:49:18 -0400 Subject: [Histonet] Frozen sections: H&E and TMA questions Message-ID: Hi, I'm at a new lab where we use Leica Infinity stains for our frozen H&E stains. I've noticed our stains are very pale and poorly differentiated. Does anyone have experience with this particular Leica product? Or do you guys have a recommendation for a better H&E stain kit? At my previous job we used a very simple staining procedure that produced excellent results. 100% EtoH, water, Mayers 3 Hematoxylin, 4 rounds of water, Eosin-Y, 4 rounds of 100% EtoH Also, does anyone have experience making frozen TMAs? I would be making cores out of snap frozen tissue, so I'm trying to think of a way of extracting cores without shattering the tissue. Thanks in advance, Phred Washington DC From Samantha.Golden at HCAHealthcare.com Sat Jul 4 07:29:24 2020 From: Samantha.Golden at HCAHealthcare.com (Samantha.Golden at HCAHealthcare.com) Date: Sat, 4 Jul 2020 12:29:24 +0000 Subject: [Histonet] Lab assistant/histotech Message-ID: <37341A94-5268-4D7A-AFE0-B591A62E3F84@HCAHealthcare.com> I was curious if there is a source, publication, report, study, general information, regarding the use of lab assistants in the histology lab. I would like the know the percentage of labs that utilize assistants versus labs with techs only. I would also like to know that for labs that do utilize assistants, what duties do they perform that the techs are exempt from. I?m simply curious to see how other labs have workflow setup. Thanks for any insight. Samantha --- Sent from Workspace ONE Boxer From garreyf at gmail.com Sat Jul 4 12:08:38 2020 From: garreyf at gmail.com (Garrey Faller) Date: Sat, 4 Jul 2020 13:08:38 -0400 Subject: [Histonet] Tissue processor errors, failures and what to do Message-ID: <0D1826B0-96F1-4F9F-AB57-FB3621AE2D99@gmail.com> Happy 4th to all. Does anyone have a procedure on what to do when a tissue processor fails or alarms. I want to learn more about the science behind tissue processing so I know what to do when the machine fails. This happened to a friend recently and I want to prevent my tissues/biopsies from being ruined. Thanks. Garrey Sent from my iPhone From mojtaba.akhtari at gmail.com Sat Jul 4 12:24:34 2020 From: mojtaba.akhtari at gmail.com (Mojtaba Akhtari) Date: Sat, 4 Jul 2020 10:24:34 -0700 Subject: [Histonet] Please, unsubscribe Message-ID: From patpxs at gmail.com Sat Jul 4 15:39:18 2020 From: patpxs at gmail.com (Patpxs) Date: Sat, 4 Jul 2020 13:39:18 -0700 Subject: [Histonet] Tissue processor errors, failures and what to do In-Reply-To: <0D1826B0-96F1-4F9F-AB57-FB3621AE2D99@gmail.com> References: <0D1826B0-96F1-4F9F-AB57-FB3621AE2D99@gmail.com> Message-ID: <6044A425-BA87-4CAB-8B66-832CEEE85A70@gmail.com> Hi Garrey, The answer is ?it depends?. What you do when a processor fails depends on the failure point. If the tissue is still in dehydrant it gets treated differently than if it fails in the intermediate solvent. Paula Sent from my iPhone > On Jul 4, 2020, at 10:08 AM, Garrey Faller via Histonet wrote: > > ?Happy 4th to all. > Does anyone have a procedure on what to do when a tissue processor fails or alarms. I want to learn more about the science behind tissue processing so I know what to do when the machine fails. This happened to a friend recently and I want to prevent my tissues/biopsies from being ruined. > Thanks. > Garrey > > Sent from my iPhone > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From eroy at illinois.edu Sat Jul 4 20:08:26 2020 From: eroy at illinois.edu (Roy, Edward J) Date: Sun, 5 Jul 2020 01:08:26 +0000 Subject: [Histonet] Fixed frozen non-paraffin mouse brain Message-ID: As a research lab, we sometimes would like to use paraformaldehyde-fixed but non-paraffin embedded tissues; paraffin embedding alters antigens and necessitates antigen retrieval, but simple fixation does not. We have done the traditional 30% sucrose before OCT and freezing, with cryostat sectioning, but results are inconsistent, sometimes producing Swiss-cheese brains. Does anybody have an alternative to 30% sucrose that is more reliable? I didn?t see anything in the Archives after a search for ?30% sucrose?. Thanks very much, Ed Roy Edward J. Roy, PhD Professor Emeritus Department of Molecular and Integrative Physiology University of Illinois at Urbana-Champaign Urbana, IL 61801 217 333-3375 From ewj at pigs.ag Sat Jul 4 20:28:10 2020 From: ewj at pigs.ag (E. Wayne Johnson) Date: Sun, 5 Jul 2020 09:28:10 +0800 Subject: [Histonet] Tissue processor errors, failures and what to do In-Reply-To: <6044A425-BA87-4CAB-8B66-832CEEE85A70@gmail.com> References: <0D1826B0-96F1-4F9F-AB57-FB3621AE2D99@gmail.com> <6044A425-BA87-4CAB-8B66-832CEEE85A70@gmail.com> Message-ID: Automation is a wonderful thing but it is only a replacement for what people used to do by hand. We have incubators that can be set to 60C and we have a Rube Goldberg-ized microwave oven with a thermal controller and relays (and the not-to-be-forgotten flyback diode) and a K-type thermal probe covered with aluminum foil (dont try this at home, kids) so we are ready for any incident or mishap with our cantankerous VIP5. There is no such thing as a convenient time for it to quit but lots of times the trouble is that it was time to change the solutions and nobody did it even though anybody could have done it. If there is nothing we can do to get the VIP5 going again pronto we finish the processing by hand. there are repair manuals for some of those machines available online or you can call the repairman.? If you are out in Bufooee somewhere the repair manual and the secret repair modes on the machine could be the difference between joy and the slough of despond. E. Wayne Johnson DVM Enable AgTech Beijing Patpxs via Histonet wrote: > Hi Garrey, > > The answer is ?it depends?. What you do when a processor fails depends on the failure point. If the tissue is still in dehydrant it gets treated differently than if it fails in the intermediate solvent. > > Paula > > Sent from my iPhone > >> On Jul 4, 2020, at 10:08 AM, Garrey Faller via Histonet wrote: >> >> ?Happy 4th to all. >> Does anyone have a procedure on what to do when a tissue processor fails or alarms. I want to learn more about the science behind tissue processing so I know what to do when the machine fails. This happened to a friend recently and I want to prevent my tissues/biopsies from being ruined. >> Thanks. >> Garrey >> >> Sent from my iPhone >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera at msu.edu Sat Jul 4 20:28:26 2020 From: portera at msu.edu (Porter, Amy) Date: Sun, 5 Jul 2020 01:28:26 +0000 Subject: [Histonet] Fixed frozen non-paraffin mouse brain In-Reply-To: References: Message-ID: You need to add sucrose to your PFA we use 4%PFA+4%Sucrose to fix and then cryoprotect in 30% sucrose which all need to be prepared in Phosphate buffer solution NO saline .... if you message me directly I would be happy to share our SOP this was a very hard thing to learn.... not a lot in technical references about this process. Sent from my iPhone > On Jul 4, 2020, at 9:09 PM, Roy, Edward J via Histonet wrote: > > ?As a research lab, we sometimes would like to use paraformaldehyde-fixed but non-paraffin embedded tissues; paraffin embedding alters antigens and necessitates antigen retrieval, but simple fixation does not. We have done the traditional 30% sucrose before OCT and freezing, with cryostat sectioning, but results are inconsistent, sometimes producing Swiss-cheese brains. Does anybody have an alternative to 30% sucrose that is more reliable? I didn?t see anything in the Archives after a search for ?30% sucrose?. > Thanks very much, > Ed Roy > > > Edward J. Roy, PhD > Professor Emeritus > Department of Molecular and Integrative Physiology > University of Illinois at Urbana-Champaign > Urbana, IL 61801 > 217 333-3375 > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.com%2Fv3%2F__http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet__%3B!!HXCxUKc!jkI5NdLT3gGvmm9DK87HJOVfSDogjkNNjK6aobOZKeIdVbQSf5Y3zN9OCgerig%24&data=01%7C01%7Cportera%40msu.edu%7C7cacd5e670c74a9bcac208d8208004ac%7C22177130642f41d9921174237ad5687d%7C0&sdata=CE67aooP%2BsILBNDemkXRjDcNpQv4XmQa7eb%2BBP4d8HA%3D&reserved=0 From tina.vanmeter at gmail.com Sun Jul 5 00:02:42 2020 From: tina.vanmeter at gmail.com (Tina Van Meter) Date: Sun, 5 Jul 2020 01:02:42 -0400 Subject: [Histonet] Fixed frozen non-paraffin mouse brain In-Reply-To: References: Message-ID: Hi Ed, I have been using the 30% sucrose technique to cryoprotect animal tissue for over 40 years without any problem. Did the tissue sink to the bottom of the specimens jar? After sinking, I blot the excess sucrose from the tissue on a paper towel before transfering to OCT. What is your procedure to freeze the tissue? Thank you, Tina Van Meter Scripps Research Histology Core Manager Jupiter, FL On Sat, Jul 4, 2020, 9:26 PM Roy, Edward J via Histonet < histonet at lists.utsouthwestern.edu> wrote: > As a research lab, we sometimes would like to use paraformaldehyde-fixed > but non-paraffin embedded tissues; paraffin embedding alters antigens and > necessitates antigen retrieval, but simple fixation does not. We have done > the traditional 30% sucrose before OCT and freezing, with cryostat > sectioning, but results are inconsistent, sometimes producing Swiss-cheese > brains. Does anybody have an alternative to 30% sucrose that is more > reliable? I didn?t see anything in the Archives after a search for ?30% > sucrose?. > Thanks very much, > Ed Roy > > > Edward J. Roy, PhD > Professor Emeritus > Department of Molecular and Integrative Physiology > University of Illinois at Urbana-Champaign > Urbana, IL 61801 > 217 333-3375 > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jkiernan at uwo.ca Sun Jul 5 00:16:25 2020 From: jkiernan at uwo.ca (John Kiernan) Date: Sun, 5 Jul 2020 05:16:25 +0000 Subject: [Histonet] Fixed frozen non-paraffin mouse brain In-Reply-To: References: Message-ID: Dear Ed, Adequate fixation is important. Formaldehyde penetrates quickly but reacts slowly with proteins. 4% formaldehyde made by depolymerizing paraformaldehyde (an insoluble high polymer) is the same as formaldehyde made by 10X dilution of formalin (a mixture of soluble low polymers). For cryoprotection the sucrose must thoroughly penetrate the fixed specimen, which must sink in the concentrated solution. Your Swiss cheese artifact is due to slow freezing. In badly frozen brains the ice crystal holes are as big as large neurons and the tissue architecture is destroyed. Unless specimens are really tiny and frozen super-fast (special methods for electron microscopy), ice crystals always form and show later as holes in the sections. You get bigger holes with bigger specimens and slower freezing. With very fast freezing, a skilled technician can get good cryostat sections even of small unfixed specimens such as muscle biopsies. These are needed for enzyme activity histochemistry methods used in diagnostic pathology and in research. Cryoprotection of fixed specimens slows the growth of ice crystals. With luck, the holes are too small to interfere with light microscope studies of sections. In neuroscience research, quite thick frozen sections of samll animals' brains have been the norm for more than 50 years. About 20 years ago I wrote a chapter that gave some quite detailed instructions and explanations, with references. (Don't do anything important just because a chapter or a review says so; check at least some of the refs!) Here is the reference. Kiernan, J. A. 2002. Freezing and fixation. Chapter 8 in Microscopy and Histology for Molecular Biologists. A User's Guide, ed. Kiernan, J. A. & Mason, I. G. pp. 103-143. London: Portland Press. ISBN 1855781417. Your university's library in Urbana might have the book. It's out-of-print with its publisher. There are used copies on the web for much less than the original price. John Kiernan Emeritus neuroanatomist and histochemist London, Canada https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html = = = ________________________________ From: Roy, Edward J via Histonet Sent: 04 July 2020 20:08 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fixed frozen non-paraffin mouse brain As a research lab, we sometimes would like to use paraformaldehyde-fixed but non-paraffin embedded tissues; paraffin embedding alters antigens and necessitates antigen retrieval, but simple fixation does not. We have done the traditional 30% sucrose before OCT and freezing, with cryostat sectioning, but results are inconsistent, sometimes producing Swiss-cheese brains. Does anybody have an alternative to 30% sucrose that is more reliable? I didn?t see anything in the Archives after a search for ?30% sucrose?. Thanks very much, Ed Roy Edward J. Roy, PhD Professor Emeritus Department of Molecular and Integrative Physiology University of Illinois at Urbana-Champaign Urbana, IL 61801 217 333-3375 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Sun Jul 5 14:25:51 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sun, 5 Jul 2020 19:25:51 +0000 Subject: [Histonet] Fixed frozen non-paraffin mouse brain Message-ID: Prof. Kiernan, as usual, provides us all with such a depth/breadth of particular information/advice. His Histological and Histochemical methods BIBLE is still my favourite read. Respect Most researchers fix in depolymerised Paraformaldehyde because someone must've originally thought: " Hang on, if we fix in commercially bought 40% Formalin, it's got 10% methanol added ( to slow rate of formaldehyde repolymerisation) ...that will compete with Formaldehyde fixation. So, we get coagulative and additive fixation. That is not good, folks....let's get pure and use depolymerised Paraformaldehyde: pure methylene glycol polymer" I am sure Professor Kiernan can correct my inaccuracies! Anyway......I've never noticed any difference: I've worked in diagnostic labs ( unfixed frozen muscle/renal/rectal bx) and also research labs ( unfixed/ fixed frozen tissues) using both fixing solutions I have not noticed any IHC/IF difference in reactivity. Many primary abs do NOT work even with fixed/unfixed frozen....some of them WILL need HIER ( at 90C rather than M/W or pressure cooker AR but, only fixed frozen of course), imho. Part of the problem is whether the antigen is linear or 3D...sorry for simplicity. I can successfully snap-freeze fixed/unfixed rat/ms brain hemispheres without using sucrose ( success measured by lack of holes at the LM level). This is because I was trained in a diagnostic lab to freeze fast but, effectively. It is a technique that requires experience for consistency of success....sometimes I fail! The reason most use 20/30% sucrose is to give poor a snap-freezing technique a chance to avoid ice-crystal artefact, as stated by Kiernan). Sucrose is no panacea.....technique is everything. My pennyworth-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From Christopher.Sheeder at seattlechildrens.org Mon Jul 6 09:44:16 2020 From: Christopher.Sheeder at seattlechildrens.org (Sheeder, Christopher) Date: Mon, 6 Jul 2020 14:44:16 +0000 Subject: [Histonet] Lab assistant/histotech In-Reply-To: <37341A94-5268-4D7A-AFE0-B591A62E3F84@HCAHealthcare.com> References: <37341A94-5268-4D7A-AFE0-B591A62E3F84@HCAHealthcare.com> Message-ID: Hi Samantha, I have used lab assistants throughout my career. Here is a list of duties they would perform: Accession specimens File blocks and slides Clean glassware Restock supplies Clean work areas Fax/scan/print reports Answer phone Recycle solvents Dump tissue Assist PA's in grossing (make cassettes/document workload) Have a great Monday! Chris Christopher Sheeder, HT(ASCP)CMQIHC AP Manager | Department of Laboratories Seattle Children?s Hospital 4800 Sand Point Way NE Seattle, WA 98105 Office: 206-987-6259 christopher.sheeder at seattlechildrens.org -----Original Message----- From: Samantha.Golden at HCAHealthcare.com Sent: Saturday, July 04, 2020 5:29 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Lab assistant/histotech I was curious if there is a source, publication, report, study, general information, regarding the use of lab assistants in the histology lab. I would like the know the percentage of labs that utilize assistants versus labs with techs only. I would also like to know that for labs that do utilize assistants, what duties do they perform that the techs are exempt from. I?m simply curious to see how other labs have workflow setup. Thanks for any insight. Samantha --- Sent from Workspace ONE Boxer CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Muhammad.Azam2 at va.gov Mon Jul 6 11:14:57 2020 From: Muhammad.Azam2 at va.gov (Azam, Muhammad) Date: Mon, 6 Jul 2020 16:14:57 +0000 Subject: [Histonet] Fee based grossing histotech Message-ID: Hi: Does anybody has reference for paying fee based grossing histotech. We used to pay a dollar amount per paraffin block. Thanks; V/r Muhammad Azam, MD Chief, Pathology & Laboratory Medicine Service (113) James H. Quillen VAMC Mountain Home, TN Phone: 423-979-3529 From Samantha.Golden at HCAHealthcare.com Mon Jul 6 11:52:21 2020 From: Samantha.Golden at HCAHealthcare.com (Samantha.Golden at HCAHealthcare.com) Date: Mon, 6 Jul 2020 16:52:21 +0000 Subject: [Histonet] Lab assistant/histotech In-Reply-To: References: <37341A94-5268-4D7A-AFE0-B591A62E3F84@HCAHealthcare.com>, Message-ID: <1792f7a42bb34d6c8e9b6a99e7c5e19e@HCAHealthcare.com> Thank you for your input. We currently have a lab assistant but need techs to help fill in the gap. Our techs do not like this, but we all are on the same team at the end of the day and working for our patients. Thanks again. Have a great day! Sam ________________________________ From: Sheeder, Christopher Sent: Monday, July 6, 2020 10:44:16 AM To: Golden Samantha - Asheville; histonet at lists.utsouthwestern.edu Subject: {EXTERNAL} RE: [Histonet] Lab assistant/histotech Hi Samantha, I have used lab assistants throughout my career. Here is a list of duties they would perform: Accession specimens File blocks and slides Clean glassware Restock supplies Clean work areas Fax/scan/print reports Answer phone Recycle solvents Dump tissue Assist PA's in grossing (make cassettes/document workload) Have a great Monday! Chris Christopher Sheeder, HT(ASCP)CMQIHC AP Manager | Department of Laboratories Seattle Children?s Hospital 4800 Sand Point Way NE Seattle, WA 98105 Office: 206-987-6259 christopher.sheeder at seattlechildrens.org -----Original Message----- From: Samantha.Golden at HCAHealthcare.com Sent: Saturday, July 04, 2020 5:29 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Lab assistant/histotech I was curious if there is a source, publication, report, study, general information, regarding the use of lab assistants in the histology lab. I would like the know the percentage of labs that utilize assistants versus labs with techs only. I would also like to know that for labs that do utilize assistants, what duties do they perform that the techs are exempt from. I?m simply curious to see how other labs have workflow setup. Thanks for any insight. Samantha --- Sent from Workspace ONE Boxer CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Timothy.Morken at ucsf.edu Mon Jul 6 12:16:22 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 6 Jul 2020 17:16:22 +0000 Subject: [Histonet] Lab assistant/histotech In-Reply-To: <1792f7a42bb34d6c8e9b6a99e7c5e19e@HCAHealthcare.com> References: <37341A94-5268-4D7A-AFE0-B591A62E3F84@HCAHealthcare.com>, <1792f7a42bb34d6c8e9b6a99e7c5e19e@HCAHealthcare.com> Message-ID: Samantha, Yes there is: Staffing Benchmarks for Histology Laboratories Rene Buesa Annals of Diagnostic Pathology Volume 14, Issue 3, June 2010, Pages 182-193 He details here how lab assistants make labs more productive by allowing trained histotechs to do the embedding, sectioning and staining and leave everything else to lab assistants. BTW, if you go to Google Scholar and search Histology RJ Buesa you will see a series of articles specific to histology laboratory management by Rene. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Samantha Golden, HT(ASCP) via Histonet Sent: Monday, July 06, 2020 9:52 AM To: Christopher.Sheeder at seattlechildrens.org; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Lab assistant/histotech Thank you for your input. We currently have a lab assistant but need techs to help fill in the gap. Our techs do not like this, but we all are on the same team at the end of the day and working for our patients. Thanks again. Have a great day! Sam ________________________________ From: Sheeder, Christopher Sent: Monday, July 6, 2020 10:44:16 AM To: Golden Samantha - Asheville; histonet at lists.utsouthwestern.edu Subject: {EXTERNAL} RE: [Histonet] Lab assistant/histotech Hi Samantha, I have used lab assistants throughout my career. Here is a list of duties they would perform: Accession specimens File blocks and slides Clean glassware Restock supplies Clean work areas Fax/scan/print reports Answer phone Recycle solvents Dump tissue Assist PA's in grossing (make cassettes/document workload) Have a great Monday! Chris Christopher Sheeder, HT(ASCP)CMQIHC AP Manager | Department of Laboratories Seattle Children's Hospital 4800 Sand Point Way NE Seattle, WA 98105 Office: 206-987-6259 christopher.sheeder at seattlechildrens.org -----Original Message----- From: Samantha.Golden at HCAHealthcare.com Sent: Saturday, July 04, 2020 5:29 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Lab assistant/histotech I was curious if there is a source, publication, report, study, general information, regarding the use of lab assistants in the histology lab. I would like the know the percentage of labs that utilize assistants versus labs with techs only. I would also like to know that for labs that do utilize assistants, what duties do they perform that the techs are exempt from. I'm simply curious to see how other labs have workflow setup. Thanks for any insight. Samantha --- Sent from Workspace ONE Boxer CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIF-g&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=ttkxt_KK5qihyzumNjUQK5P-qnv8y-N4QobaL4fKQuQ&s=QYGbxW_sp8tjUqqrRT0r4fBCWIbkNx8IAa6IqHUGKSQ&e= From jkiernan at uwo.ca Tue Jul 7 10:19:39 2020 From: jkiernan at uwo.ca (John Kiernan) Date: Tue, 7 Jul 2020 15:19:39 +0000 Subject: [Histonet] Fixed frozen non-paraffin mouse brain In-Reply-To: References: Message-ID: And a very good pennyworth it is, Carl! You wrote, "... someone must've originally thought: 'Hang on, if we fix in commercially bought 40% Formalin, it's got 10% methanol added (to slow rate of formaldehyde repolymerisation) ...that will compete with formaldehyde fixation. So, we get coagulative and additive fixation. That is not good, folks....let's get pure and use depolymerised paraformaldehyde: pure methylene glycol polymer'". That's almost how it came about: let's get pure. A fixative made from PFA should have the same composition every time it is freshly prepared. The 10% of methanol (MeOH) in formalin (40% HCHO) isn't enough to coagulate proteins, and neither is the 1% MeOH in 10% formalin (with 4% HCHO). You need 60-70% alcohol to coagulate proteins, viruses etc. Formalin also contains some formic acid; the amount increases with age, from oxidation of the aldehyde by air. Dilution with water always gives an acidic solution. Marble chips can be added bring the pH up to neutrality. Buffering also takes care of the formic acid and can provide a neutral (pH7) or a "physiological" (pH7.4) fixative solution. The usual phosphate buffer also makes the fixative solution approximately iso-osmotic with mammalian extracellular fluid. Before the 1960s, dilution of formalin with with saline (0.9% NaCl) provided "formal saline", which had some advantages over 4% aqueous formaldehyde. See books by J. R. Baker, which are available as free downloads from http://archive.com. Polymerization also increases with age. That's why you see a white precipitate in bottles of formalin stored for a long time. The precipitate is paraformaldehyde (PFA); its presence reduces the amount of formaldehyde that can be easily released by simple dilution of the formalin with water. According to R. Cares (1945: A note on stored formaldehyde and its easy reconditioning. J. Tech. Methods & Bull. Int. Ass. Med. Museums 25, 67-70), milky formalin can be cleared by autoclaving, for 30 m in Kilner jars. I wonder if anyone else has done this? John Kiernan Anatomy & Cell Biology UWO, London, Canada = = = ________________________________ From: Hobbs, Carl via Histonet Sent: 05 July 2020 14:25 To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Fixed frozen non-paraffin mouse brain Prof. Kiernan, as usual, provides us all with such a depth/breadth of particular information/advice. His Histological and Histochemical methods BIBLE is still my favourite read. Respect Most researchers fix in depolymerised Paraformaldehyde because someone must've originally thought: " Hang on, if we fix in commercially bought 40% Formalin, it's got 10% methanol added ( to slow rate of formaldehyde repolymerisation) ...that will compete with Formaldehyde fixation. So, we get coagulative and additive fixation. That is not good, folks....let's get pure and use depolymerised Paraformaldehyde: pure methylene glycol polymer" I am sure Professor Kiernan can correct my inaccuracies! Anyway......I've never noticed any difference: I've worked in diagnostic labs ( unfixed frozen muscle/renal/rectal bx) and also research labs ( unfixed/ fixed frozen tissues) using both fixing solutions I have not noticed any IHC/IF difference in reactivity. Many primary abs do NOT work even with fixed/unfixed frozen....some of them WILL need HIER ( at 90C rather than M/W or pressure cooker AR but, only fixed frozen of course), imho. Part of the problem is whether the antigen is linear or 3D...sorry for simplicity. I can successfully snap-freeze fixed/unfixed rat/ms brain hemispheres without using sucrose ( success measured by lack of holes at the LM level). This is because I was trained in a diagnostic lab to freeze fast but, effectively. It is a technique that requires experience for consistency of success....sometimes I fail! The reason most use 20/30% sucrose is to give poor a snap-freezing technique a chance to avoid ice-crystal artefact, as stated by Kiernan). Sucrose is no panacea.....technique is everything. My pennyworth-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Tue Jul 7 13:29:38 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Tue, 7 Jul 2020 18:29:38 +0000 Subject: [Histonet] Fixed frozen non-paraffin mouse brain Message-ID: Chuckle Thank you, John Kiernan Yes...I appreciate your filling in them gaps. I do understand/know them....having read, over the years many of the Giants of Histology who gave me such inciteful/usable knowledge, including yourself. I just didn't want to bloat my "pennyworth" otherwise it would become several Poundsworth , chuckle. I am not worthy.... I recall having pp in unbuffered Formalin...we chucked it Well, in those days one emptied it down the sink! Sure, re Formic acid.... However, imho...that is a more theoretical problem, as Formalin was used up within a couple of weeks...in any of my Labs I used to check the pH once a week. In practice, there is much leeway in the use of Formalin....provided one is knowlegable regarding all the parameters? Sure, it is not the "best" fixing fluid but....it all depends, as you intimated, on one's needs ( eg: Ultrastructural integrity v immunoreactivity) Thank you very much for your elucidations I always learn more....am happy to I have the ORANGE book ( at work..I am on furlough) ...grrr...I fail to recall the most excellent author...on fixation. 1st edition I thoroughly enjoy/ed reading it It is another of my "Bibles"....it expands my mind just like any Bible should...imho. A bible is like a learning curve: one must go back to it when in doubt but.....go forward when in no doubt but, supported/stayed by that original knowledge Hence, I can see further by standing on the shoulders of the giants that came before me? Paraphrasingly...surely. Your opinion re the Orange-covered book ? Sure, there are many resources/publications regarding fixation...most are idiosyncratic....imho. Respectfully Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From jaylundgren at gmail.com Tue Jul 7 16:09:21 2020 From: jaylundgren at gmail.com (Jay Lundgren) Date: Tue, 7 Jul 2020 16:09:21 -0500 Subject: [Histonet] Fee based grossing histotech In-Reply-To: References: Message-ID: I think it's more fair to pay them a share of the professional fee, based on the CPT code of the tissue being grossed. That's basically how a PA working for a group gets paid (although most are salaried), and you are asking a histotech to do a PA's job. From making at ufl.edu Wed Jul 8 10:32:49 2020 From: making at ufl.edu (King,Michael A) Date: Wed, 8 Jul 2020 15:32:49 +0000 Subject: [Histonet] Histonet Digest, Vol 200, Issue 5 In-Reply-To: References: Message-ID: <1594222369355.47223@ufl.edu> Happy to second that opinion, that was probably the single most valuable training and reference text in my career as a research neurobiologist (also hunt down his archived Histonet posts on formaldehyde fixation, essential reading for lab pros). Others apparently agreed, as I had to replace a couple copies that got 'lost' over the years, but that was reason to get the latest editions. It was good to see him still responding here, and hope he will be gladdened to know that dozens of students who passed through my lab came to appreciate and benefit from his terrific approach to the technology. --------------- 5 Jul 2020 19:25:51 +0000 From: "Hobbs, Carl" To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Fixed frozen non-paraffin mouse brain Message-ID: Content-Type: text/plain; charset="iso-8859-1" Prof. Kiernan, as usual, provides us all with such a depth/breadth of particular information/advice. His Histological and Histochemical methods BIBLE is still my favourite read. From ajju33 at gmail.com Wed Jul 8 12:46:52 2020 From: ajju33 at gmail.com (Muhammad Azam) Date: Wed, 8 Jul 2020 13:46:52 -0400 Subject: [Histonet] Histonet Digest, Vol 200, Issue 5 In-Reply-To: <1594222369355.47223@ufl.edu> References: <1594222369355.47223@ufl.edu> Message-ID: <0D16983D-37DF-4517-A0D7-72BB09A57F53@gmail.com> I take it u r talking about Prof. Kieran. We r lucky to have access to Prof Kiernan?s vast experience and wisdom. Dr Azam Sent from my iPhone > On Jul 8, 2020, at 11:49 AM, King,Michael A via Histonet wrote: > > ?Happy to second that opinion, that was probably the single most valuable training and reference text in my career as a research neurobiologist (also hunt down his archived Histonet posts on formaldehyde fixation, essential reading for lab pros). Others apparently agreed, as I had to replace a couple copies that got 'lost' over the years, but that was reason to get the latest editions. It was good to see him still responding here, and hope he will be gladdened to know that dozens of students who passed through my lab came to appreciate and benefit from his terrific approach to the technology. > --------------- > 5 Jul 2020 19:25:51 +0000 > From: "Hobbs, Carl" > To: "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Fixed frozen non-paraffin mouse brain > Message-ID: > > > Content-Type: text/plain; charset="iso-8859-1" > > Prof. Kiernan, as usual, provides us all with such a depth/breadth of particular information/advice. > His Histological and Histochemical methods BIBLE is still my favourite read. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Wed Jul 8 13:23:40 2020 From: rsrichmond at gmail.com (Bob Richmond) Date: Wed, 8 Jul 2020 14:23:40 -0400 Subject: [Histonet] AFIP Manual In-Reply-To: References: Message-ID: > > The edition of the "AFIP Manual" - Manual of Histologic Staining Methods > of the Armed Forces Institute of Pathology, Third Edition - most of us are > familiar with was edited by Lee G. Luna. I recall buying a copy of it in > 1968 when I was a resident. I now have a copy of what I think is the last > printing, which I bought in 1992. > Googling it, I found that this book is long out of print, extremely scarce and expensive, and I'm not sure it's available online. I think it should be reprinted, or at least made available online. (I note that Dezna Sheehan's somewhat later book is also out of print.) The publication history of the AFIP manual before 1968 is rather murky. The "first edition", never formally published, was loose mimeographed notes written by (Miss) Mary Francis Gridley (1908-1954), which after her early death (of acute leukemia) was published with no definite authorship in 1957, and probably revised in 1960. I recall seeing this edition more than 50 years ago, but don't remember the color of the cover. I can't find an online record of the 1960 edition. The 1957 edition is available online from Google Books at https://tinyurl.com/y8wosf2j Apparently there was a 4th edition in 1992, but I found no record of it. Histotechnologists in my day regarded the book as well-nigh scriptural. It was not a very satisfactory reference for a pathologist, since it did not go into detail either on theory or on interpretation. I much preferred R.D. Lillie's Histologic Technic and Practical Histochemistry (3rd ed., 1965), but it wasn't formatted for practical use as a manual. I have several more books of that vintage, none entirely satisfactory. Bob Richmond Samurai Pathologist Maryville TN From ASelf at tidelandshealth.org Wed Jul 8 14:26:00 2020 From: ASelf at tidelandshealth.org (Amy Self) Date: Wed, 8 Jul 2020 19:26:00 +0000 Subject: [Histonet] FNA Procedures Message-ID: Hope everyone is having a wonderful Wednesday... We (histology) have been doing what seems like tons of FNA procedures (Radiology/EBUS\EUS) here lately as well as BM procedures. With all of the Covid going on around us we are working short staffed and doing the best we can with what we have, but it seems like the physicians are scheduling these procedure's around what works best for them and this seems to be creating some issues here in pathology, especially trying to work between two hospitals because they are scheduling these procedure's when we don't seem to have staff available or they will call and say we have an add-on at the last minute and will need pathology when in fact they end not needing our services. Oh yea and we do not have an on-site cytotech so histology techs are the ones that are helping assist with FNA's. I was wondering what other facilities are doing as far as working with the other departments with these FNA procedures after hours. Do you have cut-off times, or have them place the aspirated material in formalin for cell block, train the nursing staff to make smears and hope they are good samples or do you just accommodate and go to the procedure if needed? I think that it's time for a SOP to be put in place. Thanks, Amy Self Senior Histology Technologist Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 Office: (843) 520-8711 aself at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From john.garratt at ciqc.ca Wed Jul 8 19:40:02 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 09 Jul 2020 00:40:02 +0000 Subject: [Histonet] FNA Procedures In-Reply-To: References: Message-ID: We published the following article a few years back in Acta Cytologica that outlines what we are doing to manage cases without on site evaluation. Stats like these help to make your case. Ultimately we found the imaging department preferred not to be waiting for lab and they were able to become more efficient. I hope this helps - John Gastrointestinal Endoscopic Ultrasound-Guided Fine-Needle Aspiration Biopsy Specimens: Adequate Diagnostic Yield and Accuracy Can Be Achieved without On-Site Evaluation Article?in?[Acta cytologica](https://www.researchgate.net/publication/journal/0001-5547_Acta_cytologica) 59(4) ? September 2015 On Wed, Jul 8, 2020 at 12:26 PM, Amy Self via Histonet wrote: > Hope everyone is having a wonderful Wednesday... > > We (histology) have been doing what seems like tons of FNA procedures (Radiology/EBUS\EUS) here lately as well as BM procedures. With all of the Covid going on around us we are working short staffed and doing the best we can with what we have, but it seems like the physicians are scheduling these procedure's around what works best for them and this seems to be creating some issues here in pathology, especially trying to work between two hospitals because they are scheduling these procedure's when we don't seem to have staff available or they will call and say we have an add-on at the last minute and will need pathology when in fact they end not needing our services. Oh yea and we do not have an on-site cytotech so histology techs are the ones that are helping assist with FNA's. > > I was wondering what other facilities are doing as far as working with the other departments with these FNA procedures after hours. Do you have cut-off times, or have them place the aspirated material in formalin for cell block, train the nursing staff to make smears and hope they are good samples or do you just accommodate and go to the procedure if needed? > > I think that it's time for a SOP to be put in place. > > Thanks, > Amy Self > Senior Histology Technologist > Tidelands Georgetown Memorial Hospital > 606 Black River Road > Georgetown, SC 29440 > Office: (843) 520-8711 > aself at tidelandshealth.org > Our mission: We help people live better lives through better health. > > NOTE: > The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From habumugisha at iue.ac.cn Wed Jul 8 21:07:29 2020 From: habumugisha at iue.ac.cn (Habumugisha) Date: Thu, 9 Jul 2020 10:07:29 +0800 (GMT+08:00) Subject: [Histonet] Request for information Message-ID: <5433eee9.25063.1733153e9db.Coremail.habumugisha@iue.ac.cn> Hello everyone, I want to confirm nanoplastic particles in zebrafish tissues using tranmission electron microscope (TEM), but I am not sure of how to prepare the sample. is there anyone has the protocol to share with me (especially about fixation,infiltration, embedding, staining and so forth) ? anything that you may have that I can refer to in the TEM sample preparation should be a tremendous help. I am looking forward to getting your good response, thanks. Regards! From amurvosh at advancederm.net Thu Jul 9 10:53:03 2020 From: amurvosh at advancederm.net (Anne Murvosh) Date: Thu, 9 Jul 2020 15:53:03 +0000 Subject: [Histonet] Frozen tissue Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7CC1737@Exchange.Advancederm.net> I'm testing a faster Mart 1 for melanoma on Mohs specimens. Its been a while since I've done any frozen tissue IHC stains and I can't remember the fixation to keep it viable for testing the next day, or same day but later on. Will it hold up if fixed in acetone and dried or do I need to refrigerate or freeze? Also how long do they last, I can't remember the protocol. Thanks Anne HT From tbraud at holyredeemer.com Thu Jul 9 11:38:53 2020 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 9 Jul 2020 16:38:53 +0000 Subject: [Histonet] Crystals forming in Hematoxylin Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C4BE42A4@HRHEX02-HOS.holyredeemer.local> Hello Histo Peeps - What are the clear crystals that form in Hematoxylin? We use Richard-Allen 7211. We love the stain, but occasionally, when a bottle has been previously opened, we find clear crystalline precipitate forming in the bottom. Today, we had a huge one (over an 2" in greatest diameter) that was quite beautiful. It had a few occlusions of purple streaks, but otherwise was clear. Doesn't seem to affect the stain, so I was just curious. Inquisitively yours, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From Valerie.Hannen at parrishmed.com Thu Jul 9 12:09:28 2020 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Thu, 9 Jul 2020 17:09:28 +0000 Subject: [Histonet] Crystals forming in Hematoxylin In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F801C4BE42A4@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F801C4BE42A4@HRHEX02-HOS.holyredeemer.local> Message-ID: Hi Terri, The crystals form during cold winter months, usually during shipping or can form if the Hematoxylin 7211 is stored in a cold environment. They also form due to the Mordant being put into solution at it's saturation point. The crystals have no adverse effect on the stain itself. If your bottle has a few crystals, just filter it and it should remain crystal free. If there are a lot, you can gently warm it, using a "hot" plate and constant stirring until the crystals go back into solution, then filter it and is should be good to go. I only learned this when I "googled" about it myself. I hope this helps!! Regards, Valerie Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Terri Braud via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 09, 2020 12:39 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL Sender] [Histonet] Crystals forming in Hematoxylin This message came from an external source. Please do not click links or open attachments if unexpected or unusual. Begin Original Message: ---------------------------------------------------------------------- Hello Histo Peeps - What are the clear crystals that form in Hematoxylin? We use Richard-Allen 7211. We love the stain, but occasionally, when a bottle has been previously opened, we find clear crystalline precipitate forming in the bottom. Today, we had a huge one (over an 2" in greatest diameter) that was quite beautiful. It had a few occlusions of purple streaks, but otherwise was clear. Doesn't seem to affect the stain, so I was just curious. Inquisitively yours, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PaPH0eDnAlaiSnq7Yv-U!vBWHyLqdMq6OOl66sorBVdmBIxsRePjH1moP6w3bOijwraARJ_4VQ8f_A8SaZWSs2RVXMg$ From paula at excaliburpathology.com Thu Jul 9 12:21:29 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Thu, 9 Jul 2020 17:21:29 +0000 (UTC) Subject: [Histonet] Crystals forming in Hematoxylin In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F801C4BE42A4@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F801C4BE42A4@HRHEX02-HOS.holyredeemer.local> Message-ID: <114995151.2633909.1594315289274@mail.yahoo.com> That is the aluminum salt precipitating out. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, July 9, 2020, 11:47:53 AM CDT, Terri Braud via Histonet wrote: Hello Histo Peeps - What are the clear crystals that form in Hematoxylin?? We use Richard-Allen 7211.? We love the stain, but occasionally, when a bottle has been previously opened, we find clear crystalline precipitate forming in the bottom.? Today, we had a huge one (over an 2" in greatest diameter) that was quite beautiful.? It had a few occlusions of purple streaks, but otherwise was clear.? Doesn't seem to affect the stain, so I was just curious. Inquisitively yours, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Thu Jul 9 13:28:54 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Thu, 9 Jul 2020 18:28:54 +0000 Subject: [Histonet] Histology text book "bibles" Message-ID: Good to read of others' xp I remember the Armed Forces book...good in parts, unlike the Curate's egg! Disbrey and Rack...wonderfully refreshing. Exceedingly well-informed person was Brenda! I reckon she swung the panel in my favour for a Histology Manager job.....YEARS ago! Imho the forerunner of the best: Kiernan Sure, neck and neck with Bancroft and Stevens....both game-changers Pearce...wonderfully instructive yet....dense? What about the 70's "bible"...I forget the English author......dash it! His surname begins with a C? Carleton! The book was THE text at that time ( in UK)...however, I found it rather didactic....no depth I could be wrong, of course! I did learn a lot from it...then found it lacing in depth...thirst for Histo teck knowledge, chuckle It gave me a seminal Taster for more, however. Met him at many London Histology discussion meetings...at one of which he dismisssed me for using DPX mountant ( cheaper for my lab/quickersetting...close RI , I recall?) Harrumph, he said....use natural resin: Canada balsam! Much closer RI....hmm What does Prof. Kiernan think re DPX v Canada balsam?? Luverly smell..tho ( C.balsam) Does anyone use CBalsam still? Also.... sure, Harry Cook has a special place in my Histology career...such a humble yet...knowledgeable person. He opened up my mind to things Histological...his depth and breadth of things Histological was...humbling and mesmeric. His Carbohydrate booklet was so good for me, when I needed it! Curious-illy Always Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From ajju33 at gmail.com Thu Jul 9 16:29:58 2020 From: ajju33 at gmail.com (Muhammad Azam) Date: Thu, 9 Jul 2020 17:29:58 -0400 Subject: [Histonet] Histology text book "bibles" In-Reply-To: References: Message-ID: <9992E63F-8B3C-4E50-A2C4-43024BCA3AB9@gmail.com> These last few posts r so wonderful and revealing. Where we came from and all the glorious history. Very nostalgic Dr Azam Sent from my iPhone > On Jul 9, 2020, at 2:44 PM, Hobbs, Carl via Histonet wrote: > > ?Good to read of others' xp > I remember the Armed Forces book...good in parts, unlike the Curate's egg! > Disbrey and Rack...wonderfully refreshing. > Exceedingly well-informed person was Brenda! > I reckon she swung the panel in my favour for a Histology Manager job.....YEARS ago! > Imho the forerunner of the best: Kiernan > Sure, neck and neck with Bancroft and Stevens....both game-changers > Pearce...wonderfully instructive yet....dense? > What about the 70's "bible"...I forget the English author......dash it! > His surname begins with a C? > Carleton! > The book was THE text at that time ( in UK)...however, I found it rather didactic....no depth > I could be wrong, of course! > I did learn a lot from it...then found it lacing in depth...thirst for Histo teck knowledge, chuckle > It gave me a seminal Taster for more, however. > Met him at many London Histology discussion meetings...at one of which he dismisssed me for using DPX mountant > ( cheaper for my lab/quickersetting...close RI , I recall?) > Harrumph, he said....use natural resin: Canada balsam! > Much closer RI....hmm > What does Prof. Kiernan think re DPX v Canada balsam?? > Luverly smell..tho ( C.balsam) > Does anyone use CBalsam still? > Also.... > sure, Harry Cook has a special place in my Histology career...such a humble yet...knowledgeable person. > He opened up my mind to things Histological...his depth and breadth of things Histological was...humbling and mesmeric. > His Carbohydrate booklet was so good for me, when I needed it! > Curious-illy > Always > > Carl > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > > 020 7848 6813 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From maxim_71 at mail.ru Fri Jul 10 12:23:53 2020 From: maxim_71 at mail.ru (=?UTF-8?B?0J/QtdGI0LrQvtCyINCc0LDQutGB0LjQvA==?=) Date: Fri, 10 Jul 2020 20:23:53 +0300 Subject: [Histonet] =?utf-8?q?Fwd=5B2=5D=3A_Re=3A__AFIP_Manual?= Message-ID: <1594401833.122036037@f750.i.mail.ru> ? Dear Bob! Free download AFIP manuals: 4th ed:? https://books.google.ru/books/about/Laboratory_methods_in_histotechnology.html?id=R1xrAAAAMAAJ&redir_esc=y 3rd ed:? https://archive.org/details/ManualHistologicalStaining/mode/2up?q=staining+methods+afip+manual 2nd ed:? https://archive.org/details/manualofhistolog00arme/page/n1/mode/2up ????? https://books.google.ru/books?id=CFRBAAAAYAAJ&printsec=frontcover&hl=ru&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false 1st ed:? https://books.google.ru/books?id=vx1rAAAAMAAJ&printsec=frontcover&hl=ru&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false Enjoy, please. Maxim Peshkov, Russia, Taganrog. ? ? -- ?????? ?????? ? ---------------------------------------------------------------------- ? -- ?????? ?????? ? ? ---------------------------------------------------------------------- ? -- ?????? ?????? ? From carl.hobbs at kcl.ac.uk Fri Jul 10 13:36:22 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 10 Jul 2020 18:36:22 +0000 Subject: [Histonet] Not forgetting... Message-ID: ...Ham's Histology! Forerunner and...still inciteful! Set the std for rigorous Histology books. What a wonderful read, still. He talks to me.... Sure, Junquiera too! A more....instant need ? Also the graphic Freeman and Bracegirdle...my students still want to take images of their images...simple( Black N white) yet...perfect! Of course they cannot. I love the simplicity of it.. Also...I cannot remember the author ...but..an orange-coloured 1st edition of Basics of chemical fixation ( not that title..I can't remember..grrr ) I love reading it tho...I don't understand most of it, chuckle ( I have it, Dear Readers...it is at work but, ...I am furloughed) Who was it written by?? Frustrated-illy Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From tony.henwood at health.nsw.gov.au Fri Jul 10 16:25:31 2020 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 10 Jul 2020 21:25:31 +0000 Subject: [Histonet] Fwd[2]: Re: AFIP Manual In-Reply-To: <1594401833.122036037@f750.i.mail.ru> References: <1594401833.122036037@f750.i.mail.ru> Message-ID: <1594416332395.84155@health.nsw.gov.au> Hi Maxim, Excellent At least this amazing piece of history can live on. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: ?????? ?????? via Histonet Sent: Saturday, 11 July 2020 03:23 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fwd[2]: Re: AFIP Manual Dear Bob! Free download AFIP manuals: 4th ed: https://books.google.ru/books/about/Laboratory_methods_in_histotechnology.html?id=R1xrAAAAMAAJ&redir_esc=y 3rd ed: https://archive.org/details/ManualHistologicalStaining/mode/2up?q=staining+methods+afip+manual 2nd ed: https://archive.org/details/manualofhistolog00arme/page/n1/mode/2up ??? https://books.google.ru/books?id=CFRBAAAAYAAJ&printsec=frontcover&hl=ru&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false 1st ed: https://books.google.ru/books?id=vx1rAAAAMAAJ&printsec=frontcover&hl=ru&source=gbs_ge_summary_r&cad=0#v=onepage&q&f=false Enjoy, please. Maxim Peshkov, Russia, Taganrog. -- ?????? ?????? ---------------------------------------------------------------------- -- ?????? ?????? ---------------------------------------------------------------------- -- ?????? ?????? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From kenu2 at comcast.net Fri Jul 10 16:56:14 2020 From: kenu2 at comcast.net (Ken Urban) Date: Fri, 10 Jul 2020 16:56:14 -0500 Subject: [Histonet] Histonet Digest, Vol 200, Issue 9 In-Reply-To: References: Message-ID: Charles Culling is the name/person you?re trying to remember. Sent from my iPhone > On Jul 10, 2020, at 12:00 PM, histonet-request at lists.utsouthwestern.edu wrote: > > ?Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Crystals forming in Hematoxylin (Hannen, Valerie) > 2. Re: Crystals forming in Hematoxylin (Paula Keene Pierce) > 3. Histology text book "bibles" (Hobbs, Carl) > 4. Re: Histology text book "bibles" (Muhammad Azam) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 9 Jul 2020 17:09:28 +0000 > From: "Hannen, Valerie" > To: 'Terri Braud' > Cc: "Histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Crystals forming in Hematoxylin > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hi Terri, > > The crystals form during cold winter months, usually during shipping or can form if the Hematoxylin 7211 is stored in a cold environment. They also form due to the Mordant being put into solution at it's saturation point. > The crystals have no adverse effect on the stain itself. If your bottle has a few crystals, just filter it and it should remain crystal free. If there are a lot, you can gently warm it, using a "hot" plate and constant stirring until the crystals go back into solution, then filter it and is should be good to go. I only learned this when I "googled" about it myself. I hope this helps!! > > Regards, > > Valerie > > > Valerie Hannen,MLT(ASCP),HTL,SU (FL) > Section Chief, Histology > Parrish Medical Center > 951 N. Washington Ave. > Titusville,Florida 32796 > T: (321)268-6333 ext. 7506 > F: (321) 268-6149 > valerie.hannen at parrishmed.com > www.parrishmed.com > > > > -----Original Message----- > From: Terri Braud via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, July 09, 2020 12:39 PM > To: histonet at lists.utsouthwestern.edu > Subject: [EXTERNAL Sender] [Histonet] Crystals forming in Hematoxylin > > > This message came from an external source. Please do not click links or open attachments if unexpected or unusual. > > Begin Original Message: > > ---------------------------------------------------------------------- > Hello Histo Peeps - What are the clear crystals that form in Hematoxylin? We use Richard-Allen 7211. We love the stain, but occasionally, when a bottle has been previously opened, we find clear crystalline precipitate forming in the bottom. Today, we had a huge one (over an 2" in greatest diameter) that was quite beautiful. It had a few occlusions of purple streaks, but otherwise was clear. Doesn't seem to affect the stain, so I was just curious. > Inquisitively yours, Terri > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PaPH0eDnAlaiSnq7Yv-U!vBWHyLqdMq6OOl66sorBVdmBIxsRePjH1moP6w3bOijwraARJ_4VQ8f_A8SaZWSs2RVXMg$ > > > > ------------------------------ > > Message: 2 > Date: Thu, 9 Jul 2020 17:21:29 +0000 (UTC) > From: Paula Keene Pierce > To: "histonet at lists.utsouthwestern.edu" > , Terri Braud > > Subject: Re: [Histonet] Crystals forming in Hematoxylin > Message-ID: <114995151.2633909.1594315289274 at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > That is the aluminum salt precipitating out. > Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com > > A sharp knife is nothing without a sharp eye. - Klingon Proverb > > On Thursday, July 9, 2020, 11:47:53 AM CDT, Terri Braud via Histonet wrote: > > Hello Histo Peeps - What are the clear crystals that form in Hematoxylin?? We use Richard-Allen 7211.? We love the stain, but occasionally, when a bottle has been previously opened, we find clear crystalline precipitate forming in the bottom.? Today, we had a huge one (over an 2" in greatest diameter) that was quite beautiful.? It had a few occlusions of purple streaks, but otherwise was clear.? Doesn't seem to affect the stain, so I was just curious. > Inquisitively yours, Terri > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 3 > Date: Thu, 9 Jul 2020 18:28:54 +0000 > From: "Hobbs, Carl" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Histology text book "bibles" > Message-ID: > > > Content-Type: text/plain; charset="iso-8859-1" > > Good to read of others' xp > I remember the Armed Forces book...good in parts, unlike the Curate's egg! > Disbrey and Rack...wonderfully refreshing. > Exceedingly well-informed person was Brenda! > I reckon she swung the panel in my favour for a Histology Manager job.....YEARS ago! > Imho the forerunner of the best: Kiernan > Sure, neck and neck with Bancroft and Stevens....both game-changers > Pearce...wonderfully instructive yet....dense? > What about the 70's "bible"...I forget the English author......dash it! > His surname begins with a C? > Carleton! > The book was THE text at that time ( in UK)...however, I found it rather didactic....no depth > I could be wrong, of course! > I did learn a lot from it...then found it lacing in depth...thirst for Histo teck knowledge, chuckle > It gave me a seminal Taster for more, however. > Met him at many London Histology discussion meetings...at one of which he dismisssed me for using DPX mountant > ( cheaper for my lab/quickersetting...close RI , I recall?) > Harrumph, he said....use natural resin: Canada balsam! > Much closer RI....hmm > What does Prof. Kiernan think re DPX v Canada balsam?? > Luverly smell..tho ( C.balsam) > Does anyone use CBalsam still? > Also.... > sure, Harry Cook has a special place in my Histology career...such a humble yet...knowledgeable person. > He opened up my mind to things Histological...his depth and breadth of things Histological was...humbling and mesmeric. > His Carbohydrate booklet was so good for me, when I needed it! > Curious-illy > Always > > Carl > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge? > Kings College London > London > SE1 1UL > ? > > 020 7848 6813 > > > ------------------------------ > > Message: 4 > Date: Thu, 9 Jul 2020 17:29:58 -0400 > From: Muhammad Azam > To: "Hobbs, Carl" > Cc: "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Histology text book "bibles" > Message-ID: <9992E63F-8B3C-4E50-A2C4-43024BCA3AB9 at gmail.com> > Content-Type: text/plain; charset=utf-8 > > These last few posts r so wonderful and revealing. Where we came from and all the glorious history. Very nostalgic > > Dr Azam > > > Sent from my iPhone > >> On Jul 9, 2020, at 2:44 PM, Hobbs, Carl via Histonet wrote: >> >> ?Good to read of others' xp >> I remember the Armed Forces book...good in parts, unlike the Curate's egg! >> Disbrey and Rack...wonderfully refreshing. >> Exceedingly well-informed person was Brenda! >> I reckon she swung the panel in my favour for a Histology Manager job.....YEARS ago! >> Imho the forerunner of the best: Kiernan >> Sure, neck and neck with Bancroft and Stevens....both game-changers >> Pearce...wonderfully instructive yet....dense? >> What about the 70's "bible"...I forget the English author......dash it! >> His surname begins with a C? >> Carleton! >> The book was THE text at that time ( in UK)...however, I found it rather didactic....no depth >> I could be wrong, of course! >> I did learn a lot from it...then found it lacing in depth...thirst for Histo teck knowledge, chuckle >> It gave me a seminal Taster for more, however. >> Met him at many London Histology discussion meetings...at one of which he dismisssed me for using DPX mountant >> ( cheaper for my lab/quickersetting...close RI , I recall?) >> Harrumph, he said....use natural resin: Canada balsam! >> Much closer RI....hmm >> What does Prof. Kiernan think re DPX v Canada balsam?? >> Luverly smell..tho ( C.balsam) >> Does anyone use CBalsam still? >> Also.... >> sure, Harry Cook has a special place in my Histology career...such a humble yet...knowledgeable person. >> He opened up my mind to things Histological...his depth and breadth of things Histological was...humbling and mesmeric. >> His Carbohydrate booklet was so good for me, when I needed it! >> Curious-illy >> Always >> >> Carl >> >> Carl Hobbs FIBMS >> Histology and Imaging Manager >> Wolfson CARD >> Guys Campus, London Bridge >> Kings College London >> London >> SE1 1UL >> >> >> 020 7848 6813 >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 200, Issue 9 > **************************************** From LRaff at uropartners.com Tue Jul 14 09:27:11 2020 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 14 Jul 2020 14:27:11 +0000 Subject: [Histonet] An occasional blog post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF173B6103@COLOEXCH01.uropartners.local> Hope everyone is well. Once in a while I like to post a blog link when there is a bit of a lab link, so here goes. http://www.chicagonow.com/downsize-maybe/2020/07/four-things-i-know-doing-crossword-puzzles-in-ink/ Lester J. Raff, MD MBA FCAP Laboratory Director UroPartners LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 Telephone 708-486-0076 Fax 708-492-0203 From Blanca.Lopez at UTSouthwestern.edu Tue Jul 14 10:08:03 2020 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Tue, 14 Jul 2020 15:08:03 +0000 Subject: [Histonet] M.O.M. Message-ID: Is anybody would like to share the protocol to run MOM from vector kit in the DAKO autostainer? ________________________________ UT Southwestern Medical Center The future of medicine, today. From relia1 at earthlink.net Tue Jul 14 11:13:47 2020 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 14 Jul 2020 12:13:47 -0400 Subject: [Histonet] RELIA Hot Histology Opportunities! Message-ID: <000001d659f9$c21827a0$464876e0$@earthlink.net> Hello Histopeeps, I hope you are enjoying your Summer!!! The only thing hotter than the temperatures are my phone lines!! My phones are ringing off the hook with exciting opportunities. Please take a look at my current openings. All of these positions are full time permanent positions with excellent salaries and great benefits. Some of these clients offer generous SIGN ON BONUSES and most of them are RELIA EXCLUSIVES!! Histopeeps, My current openings are located in: ? California ? Kentucky ? North Carolina ? Mohs Tech!!! ? South Carolina ? Georgia ? Mohs Tech!! We have new opportunities coming in daily!! If you or anyone you know might be interested in any of these opportunities please contact me ASAP on my cell at 407-353-5070 or I can be reached at relia1 at earthlink.net or toll free at 866-607-3542. Refer a friend earn a bonus $$$ Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From tgenade at gmail.com Tue Jul 14 11:52:03 2020 From: tgenade at gmail.com (Tyrone Genade) Date: Tue, 14 Jul 2020 12:52:03 -0400 Subject: [Histonet] stain to demonstrate silver ions in tissue? Message-ID: Hello, A colleague wants to determine the location and relative abundance of silver impregnation in tissue samples (skin). Is there an histological method for this? I know silver will stain the tissue all by itself, but it is important we are able to identify the limit of the penetration of the silver, which will likely be greater than we could detect by eye from silver's natural tendency to react with protein. Silver apparently slightly enhances the staining with DAB. My experience with Ni/Co intensification is that this metal catalyzed process happens without the need for HRP. So after blocking the silver + H2O2 should cause the DAB to precipitate locally at sites of Ag accumulation. Any thoughts on this idea? Thanks -- Tyrone Genade Johnson City, Tennessee tel: (+1) 712 230 4101 ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. *Don't panic if I don't reply to your emails sent over the weekend. I don't usually have time to check my email over the weekend and will get back to you on Monday.* From carl.hobbs at kcl.ac.uk Tue Jul 14 12:45:59 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Tue, 14 Jul 2020 17:45:59 +0000 Subject: [Histonet] An occasional blog post Message-ID: Strange/coincidence that your link has my name in it, chuckle I can't access the Chicago Tribune link.... Or, is it a scam?? Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 From ariel at cta-lab.com Tue Jul 21 10:37:54 2020 From: ariel at cta-lab.com (Ariel Liberda) Date: Tue, 21 Jul 2020 15:37:54 +0000 Subject: [Histonet] Lab pregnancy protocol? Message-ID: Hello everyone! I had an peer announce that she is expecting! While it's a very exiting time here, this lab doesn't seem to have a protocol in place for pregnant histotechs. A quick google search doesn't turn up much either. Does anyone know of a clear protocol or can anyone point me in the right direction of one? SDS's mostly do not mention pregnancy. I look forward to your replies! -Ari -- Ariel Liberda Lead Histotech CTALab c. [503.906.7300](https://urldefense.proofpoint.com/v2/url?u=https-3A__webmail.networksolutionsemail.com_edgedesk_cgi-2Dbin_tel-3A503.906.7300&d=DQMFAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=HKyZElUZ9A6Yyf6DYydB4h3JuSpSmPINBXYcEI2kzIM&m=kk9btPrCg75YkgEZBePfoDnCBgWXID9HnQfCIK51c80&s=Sd5gQGqV6e2f-nZgaLPMCVPVBCSZEDZQCN-XstC2-74&e=) | f. [503.245.8219](https://urldefense.proofpoint.com/v2/url?u=https-3A__webmail.networksolutionsemail.com_edgedesk_cgi-2Dbin_tel-3A503.245.8219&d=DQMFAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=HKyZElUZ9A6Yyf6DYydB4h3JuSpSmPINBXYcEI2kzIM&m=kk9btPrCg75YkgEZBePfoDnCBgWXID9HnQfCIK51c80&s=Ba4mH8xxABXuh3rvnefIcx6NSVFWm_tOxUI1yrS533k&e=) 12254 SW Garden Pl. | Tigard, Oregon 97223 Your skin, hair and nail pathology experts! From Sharon at nsh.org Tue Jul 21 12:36:41 2020 From: Sharon at nsh.org (Sharon Kneebone) Date: Tue, 21 Jul 2020 17:36:41 +0000 Subject: [Histonet] Lab pregnancy protocol? In-Reply-To: References: Message-ID: Hi, Here is a blog post on pregnancy in the lab, based on an NSH poster presentation by the author. https://www.fixationonhistology.com/post/my-pregnancy-in-the-lab-researching-safety-considerations Sharon H. Kneebone, CAE, IOM Executive Director P: 443-535-4060 F: 443-535-4055 E: sharon at nsh.org nsh.org? |histoconvention.org Check out NSH?s Covid-19 for Histotechs Resource Page Register for the Virtual Symposium/Convention, October 13-15th Join the conversation:? The Block |Twitter |LinkedIn |Facebook -----Original Message----- From: Ariel Liberda Sent: Tuesday, July 21, 2020 11:38 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Lab pregnancy protocol? Hello everyone! I had an peer announce that she is expecting! While it's a very exiting time here, this lab doesn't seem to have a protocol in place for pregnant histotechs. A quick google search doesn't turn up much either. Does anyone know of a clear protocol or can anyone point me in the right direction of one? SDS's mostly do not mention pregnancy. I look forward to your replies! -Ari -- Ariel Liberda Lead Histotech CTALab c. [503.906.7300](https://urldefense.proofpoint.com/v2/url?u=https-3A__webmail.networksolutionsemail.com_edgedesk_cgi-2Dbin_tel-3A503.906.7300&d=DQMFAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=HKyZElUZ9A6Yyf6DYydB4h3JuSpSmPINBXYcEI2kzIM&m=kk9btPrCg75YkgEZBePfoDnCBgWXID9HnQfCIK51c80&s=Sd5gQGqV6e2f-nZgaLPMCVPVBCSZEDZQCN-XstC2-74&e=) | f. [503.245.8219](https://urldefense.proofpoint.com/v2/url?u=https-3A__webmail.networksolutionsemail.com_edgedesk_cgi-2Dbin_tel-3A503.245.8219&d=DQMFAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=HKyZElUZ9A6Yyf6DYydB4h3JuSpSmPINBXYcEI2kzIM&m=kk9btPrCg75YkgEZBePfoDnCBgWXID9HnQfCIK51c80&s=Ba4mH8xxABXuh3rvnefIcx6NSVFWm_tOxUI1yrS533k&e=) 12254 SW Garden Pl. | Tigard, Oregon 97223 Your skin, hair and nail pathology experts! From Erin.Martin at ucsf.edu Tue Jul 21 20:13:56 2020 From: Erin.Martin at ucsf.edu (Martin, Erin) Date: Wed, 22 Jul 2020 01:13:56 +0000 Subject: [Histonet] Warm formalin Message-ID: Hello everyone! We have a referring clinician that is concerned about leaving his specimens in an outdoor lockbox in the summer because the formalin will get hot. I don't think that having some specimens in formalin in hot weather would cause any problems but I can't find any references one way or another. Does anyone have any policies regarding this? Thanks so much! Erin Martin, Histology Supervisor UCSF Dermatopathology & Oral Pathology Service Phone: 415-3537248 | Fax: 415-353-7543 CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. From patpxs at gmail.com Wed Jul 22 00:04:15 2020 From: patpxs at gmail.com (Patpxs) Date: Tue, 21 Jul 2020 22:04:15 -0700 Subject: [Histonet] Warm formalin In-Reply-To: References: Message-ID: <75291F70-8ABE-48F0-A373-CA2D76A8F98C@gmail.com> Hi Erin, Often heat is applied to formalin to speed up fixation. That said there is probably a temperature point where it goes from fixing tissue to cooking it. Paula Sent from my iPhone > On Jul 21, 2020, at 6:14 PM, Martin, Erin via Histonet wrote: > > ?Hello everyone! > > We have a referring clinician that is concerned about leaving his specimens in an outdoor lockbox in the summer because the formalin will get hot. I don't think that having some specimens in formalin in hot weather would cause any problems but I can't find any references one way or another. Does anyone have any policies regarding this? > > Thanks so much! > > > Erin Martin, Histology Supervisor > > UCSF Dermatopathology & Oral Pathology Service > > Phone: 415-3537248 | Fax: 415-353-7543 > > > CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garreyf at gmail.com Wed Jul 22 06:03:04 2020 From: garreyf at gmail.com (Garrey Faller) Date: Wed, 22 Jul 2020 07:03:04 -0400 Subject: [Histonet] Warm formalin In-Reply-To: <75291F70-8ABE-48F0-A373-CA2D76A8F98C@gmail.com> References: <75291F70-8ABE-48F0-A373-CA2D76A8F98C@gmail.com> Message-ID: <404245D4-1971-43DF-9991-37AD4F65AECC@gmail.com> I agree with Paula. I think the doc has a legitimate concern. Garrey Sent from my iPhone > On Jul 22, 2020, at 1:20 AM, Patpxs via Histonet wrote: > > ?Hi Erin, > > Often heat is applied to formalin to speed up fixation. That said there is probably a temperature point where it goes from fixing tissue to cooking it. > > Paula > > Sent from my iPhone > >> On Jul 21, 2020, at 6:14 PM, Martin, Erin via Histonet wrote: >> >> ?Hello everyone! >> >> We have a referring clinician that is concerned about leaving his specimens in an outdoor lockbox in the summer because the formalin will get hot. I don't think that having some specimens in formalin in hot weather would cause any problems but I can't find any references one way or another. Does anyone have any policies regarding this? >> >> Thanks so much! >> >> >> Erin Martin, Histology Supervisor >> >> UCSF Dermatopathology & Oral Pathology Service >> >> Phone: 415-3537248 | Fax: 415-353-7543 >> >> >> CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Wed Jul 22 12:55:14 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Wed, 22 Jul 2020 17:55:14 +0000 Subject: [Histonet] Warm formalin Message-ID: Depends on what you are doing with the sections. IHC or just dye -staining? Sure...too hot ( cooking) is not recommended, as stated Also stated is that high -temp fixation may also be used with no deleterious effects as long as the fixation time is not extended. However, RT -ish even for a week won't be a problem...imho Needs must? Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From Christopher.Sheeder at seattlechildrens.org Wed Jul 22 13:17:12 2020 From: Christopher.Sheeder at seattlechildrens.org (Sheeder, Christopher) Date: Wed, 22 Jul 2020 18:17:12 +0000 Subject: [Histonet] Warm formalin In-Reply-To: References: Message-ID: <10666c42ebb04ecf8c8f6b2a8b1e6913@seattlechildrens.org> How hot are we talking about? San Francisco hot or Phoenix hot? Christopher Sheeder, HT(ASCP)CMQIHC Histology Supervisor | Department of Laboratories Seattle Children's Hospital 4800 Sand Point Way NE Seattle, WA 98105 Office: 206-987-6259 christopher.sheeder at seattlechildrens.org -----Original Message----- From: Martin, Erin Sent: Tuesday, July 21, 2020 6:14 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Warm formalin Hello everyone! We have a referring clinician that is concerned about leaving his specimens in an outdoor lockbox in the summer because the formalin will get hot. I don't think that having some specimens in formalin in hot weather would cause any problems but I can't find any references one way or another. Does anyone have any policies regarding this? Thanks so much! Erin Martin, Histology Supervisor UCSF Dermatopathology & Oral Pathology Service Phone: 415-3537248 | Fax: 415-353-7543 CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From lynettepav at gmail.com Wed Jul 22 13:19:53 2020 From: lynettepav at gmail.com (Lynette Pavelich) Date: Wed, 22 Jul 2020 14:19:53 -0400 Subject: [Histonet] Warm formalin In-Reply-To: References: Message-ID: I would suggest to always refer to your reagent?s IFU insert. This will advise at what temperature you should use/store. All inspectors (CAP, JC, CLIA, etc.) will make you adhere to these specifications. Unless you do a well documented validation study that goes outside of these restrictions from the IFU that proves no patient harm, you honestly must go by the IFU recommendations. This would apply to all of our stains/reagents/solutions/antibodies. Our world is becoming more restrictive?? hope this helps, Lynette Pavelich, HT(ASCP), QIHC > On Jul 22, 2020, at 1:55 PM, Hobbs, Carl via Histonet wrote: > > Depends on what you are doing with the sections. > IHC or just dye -staining? > Sure...too hot ( cooking) is not recommended, as stated > Also stated is that high -temp fixation may also be used with no deleterious effects as long as the fixation time is not extended. > However, RT -ish even for a week won't be a problem...imho > Needs must? > > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > > 020 7848 6813 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Wed Jul 22 13:27:55 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Wed, 22 Jul 2020 18:27:55 +0000 Subject: [Histonet] Warm formalin In-Reply-To: References: , Message-ID: You a re right, Lynette De rigueur for Diagnostic labs! My apologies for forgetting that ( I am now in research labs where...it is less restricted, unfortunately). Essential to be well-documented/adherent to SOPs. Respectful-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From: Lynette Pavelich Sent: 22 July 2020 19:19 To: Hobbs, Carl Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Warm formalin ? I would suggest to always refer to your reagent?s IFU insert. This will advise at what temperature you should use/store. All inspectors (CAP, JC, CLIA, etc.) will make you adhere to these specifications. Unless you do a well documented validation study that goes outside of these restrictions from the IFU that proves no patient harm, you honestly must go by the IFU recommendations. This would apply to all of our stains/reagents/solutions/antibodies. Our world is becoming more restrictive?? hope this helps, Lynette Pavelich, HT(ASCP), QIHC > On Jul 22, 2020, at 1:55 PM, Hobbs, Carl via Histonet wrote: > > Depends on what you are doing with the sections. > IHC or just dye -staining? > Sure...too hot ( cooking) is not recommended, as stated > Also stated is that high -temp fixation may also be used with no deleterious effects as long as the fixation time is not extended. > However, RT -ish even for a week won't be a problem...imho > Needs must? > > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > > 020 7848 6813 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C2915a105cfa5436de65e08d82e6bd68b%7C8370cf1416f34c16b83c724071654356%7C0&sdata=YZGiBCOwM5NmjBuxHh%2B%2FuhltOh%2F0HxJs5Kb8FaurKQM%3D&reserved=0 From lynettepav at gmail.com Wed Jul 22 13:46:22 2020 From: lynettepav at gmail.com (Lynette Pavelich) Date: Wed, 22 Jul 2020 14:46:22 -0400 Subject: [Histonet] Warm formalin In-Reply-To: References: Message-ID: <49119531-EED9-45FA-845B-8FDB0BA36460@gmail.com> Carl, My only other thought is to do a documented study of the temperature of these outside storage boxes (they can be little hot/cold boxes depending on your location. Because, according to the inspectors, you must be able to assure temperature is within the required temps prior to receiving, including transportation. (Pre-analytical) So, say you do a study of the temperatures during the hottest time of the day of the boxes for a period of time (30 days of the hottest month(s)) to give an indication of a potential problem, and document. That would be one portion of pre-analytical. Then temperature during transportation (cars/vans can get quite hot/cold also needs to be addressed and documented daily just like the temperature logs in the labs. Many labs are getting cited for not providing good pre-analytical evidence. Courier services have to have temperature monitoring systems in place to provide as evidence. Some are quite savvy with alarm systems in place with monitoring. (ShipCom for example) So much documentation these days?? Lynette > On Jul 22, 2020, at 2:27 PM, Hobbs, Carl wrote: > > You a re right, Lynette > De rigueur for Diagnostic labs! > My apologies for forgetting that ( I am now in research labs where...it is less restricted, unfortunately). > Essential to be well-documented/adherent to SOPs. > Respectful-illy > > Carl > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > > 020 7848 6813 > > > From: Lynette Pavelich > Sent: 22 July 2020 19:19 > To: Hobbs, Carl > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Warm formalin > > I would suggest to always refer to your reagent?s IFU insert. This will advise at what temperature you should use/store. All inspectors (CAP, JC, CLIA, etc.) will make you adhere to these specifications. > Unless you do a well documented validation study that goes outside of these restrictions from the IFU that proves no patient harm, you honestly must go by the IFU recommendations. This would apply to all of our stains/reagents/solutions/antibodies. > Our world is becoming more restrictive?? > > hope this helps, > Lynette Pavelich, HT(ASCP), QIHC > > >> On Jul 22, 2020, at 1:55 PM, Hobbs, Carl via Histonet wrote: >> >> Depends on what you are doing with the sections. >> IHC or just dye -staining? >> Sure...too hot ( cooking) is not recommended, as stated >> Also stated is that high -temp fixation may also be used with no deleterious effects as long as the fixation time is not extended. >> However, RT -ish even for a week won't be a problem...imho >> Needs must? >> >> >> >> Carl Hobbs FIBMS >> Histology and Imaging Manager >> Wolfson CARD >> Guys Campus, London Bridge >> Kings College London >> London >> SE1 1UL >> >> >> 020 7848 6813 >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C2915a105cfa5436de65e08d82e6bd68b%7C8370cf1416f34c16b83c724071654356%7C0&sdata=YZGiBCOwM5NmjBuxHh%2B%2FuhltOh%2F0HxJs5Kb8FaurKQM%3D&reserved=0 From ajju33 at gmail.com Wed Jul 22 14:10:44 2020 From: ajju33 at gmail.com (Muhammad Azam) Date: Wed, 22 Jul 2020 15:10:44 -0400 Subject: [Histonet] Warm formalin In-Reply-To: <49119531-EED9-45FA-845B-8FDB0BA36460@gmail.com> References: <49119531-EED9-45FA-845B-8FDB0BA36460@gmail.com> Message-ID: And IFU would be what Sent from my iPhone > On Jul 22, 2020, at 3:02 PM, Lynette Pavelich via Histonet wrote: > > ?Carl, > My only other thought is to do a documented study of the temperature of these outside storage boxes (they can be little hot/cold boxes depending on your location. Because, according to the inspectors, you must be able to assure temperature is within the required temps prior to receiving, including transportation. (Pre-analytical) > So, say you do a study of the temperatures during the hottest time of the day of the boxes for a period of time (30 days of the hottest month(s)) to give an indication of a potential problem, and document. That would be one portion of pre-analytical. > > Then temperature during transportation (cars/vans can get quite hot/cold also needs to be addressed and documented daily just like the temperature logs in the labs. Many labs are getting cited for not providing good pre-analytical evidence. Courier services have to have temperature monitoring systems in place to provide as evidence. Some are quite savvy with alarm systems in place with monitoring. (ShipCom for example) > > So much documentation these days?? > > Lynette > > >> On Jul 22, 2020, at 2:27 PM, Hobbs, Carl wrote: >> >> You a re right, Lynette >> De rigueur for Diagnostic labs! >> My apologies for forgetting that ( I am now in research labs where...it is less restricted, unfortunately). >> Essential to be well-documented/adherent to SOPs. >> Respectful-illy >> >> Carl >> >> >> Carl Hobbs FIBMS >> Histology and Imaging Manager >> Wolfson CARD >> Guys Campus, London Bridge >> Kings College London >> London >> SE1 1UL >> >> >> 020 7848 6813 >> >> >> From: Lynette Pavelich >> Sent: 22 July 2020 19:19 >> To: Hobbs, Carl >> Cc: histonet at lists.utsouthwestern.edu >> Subject: Re: [Histonet] Warm formalin >> >> I would suggest to always refer to your reagent?s IFU insert. This will advise at what temperature you should use/store. All inspectors (CAP, JC, CLIA, etc.) will make you adhere to these specifications. >> Unless you do a well documented validation study that goes outside of these restrictions from the IFU that proves no patient harm, you honestly must go by the IFU recommendations. This would apply to all of our stains/reagents/solutions/antibodies. >> Our world is becoming more restrictive?? >> >> hope this helps, >> Lynette Pavelich, HT(ASCP), QIHC >> >> >>>> On Jul 22, 2020, at 1:55 PM, Hobbs, Carl via Histonet wrote: >>> >>> Depends on what you are doing with the sections. >>> IHC or just dye -staining? >>> Sure...too hot ( cooking) is not recommended, as stated >>> Also stated is that high -temp fixation may also be used with no deleterious effects as long as the fixation time is not extended. >>> However, RT -ish even for a week won't be a problem...imho >>> Needs must? >>> >>> >>> >>> Carl Hobbs FIBMS >>> Histology and Imaging Manager >>> Wolfson CARD >>> Guys Campus, London Bridge >>> Kings College London >>> London >>> SE1 1UL >>> >>> >>> 020 7848 6813 >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C2915a105cfa5436de65e08d82e6bd68b%7C8370cf1416f34c16b83c724071654356%7C0&sdata=YZGiBCOwM5NmjBuxHh%2B%2FuhltOh%2F0HxJs5Kb8FaurKQM%3D&reserved=0 > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lynettepav at gmail.com Wed Jul 22 14:12:06 2020 From: lynettepav at gmail.com (Lynette Pavelich) Date: Wed, 22 Jul 2020 15:12:06 -0400 Subject: [Histonet] Warm formalin In-Reply-To: References: <49119531-EED9-45FA-845B-8FDB0BA36460@gmail.com> Message-ID: (Instructions For Use) The paperwork that you receive with each reagent/solution/stain, etc. > On Jul 22, 2020, at 3:10 PM, Muhammad Azam wrote: > > And IFU would be what > > Sent from my iPhone > >> On Jul 22, 2020, at 3:02 PM, Lynette Pavelich via Histonet wrote: >> >> ?Carl, >> My only other thought is to do a documented study of the temperature of these outside storage boxes (they can be little hot/cold boxes depending on your location. Because, according to the inspectors, you must be able to assure temperature is within the required temps prior to receiving, including transportation. (Pre-analytical) >> So, say you do a study of the temperatures during the hottest time of the day of the boxes for a period of time (30 days of the hottest month(s)) to give an indication of a potential problem, and document. That would be one portion of pre-analytical. >> >> Then temperature during transportation (cars/vans can get quite hot/cold also needs to be addressed and documented daily just like the temperature logs in the labs. Many labs are getting cited for not providing good pre-analytical evidence. Courier services have to have temperature monitoring systems in place to provide as evidence. Some are quite savvy with alarm systems in place with monitoring. (ShipCom for example) >> >> So much documentation these days?? >> >> Lynette >> >> >>> On Jul 22, 2020, at 2:27 PM, Hobbs, Carl wrote: >>> >>> You a re right, Lynette >>> De rigueur for Diagnostic labs! >>> My apologies for forgetting that ( I am now in research labs where...it is less restricted, unfortunately). >>> Essential to be well-documented/adherent to SOPs. >>> Respectful-illy >>> >>> Carl >>> >>> >>> Carl Hobbs FIBMS >>> Histology and Imaging Manager >>> Wolfson CARD >>> Guys Campus, London Bridge >>> Kings College London >>> London >>> SE1 1UL >>> >>> >>> 020 7848 6813 >>> >>> >>> From: Lynette Pavelich >>> Sent: 22 July 2020 19:19 >>> To: Hobbs, Carl >>> Cc: histonet at lists.utsouthwestern.edu >>> Subject: Re: [Histonet] Warm formalin >>> >>> I would suggest to always refer to your reagent?s IFU insert. This will advise at what temperature you should use/store. All inspectors (CAP, JC, CLIA, etc.) will make you adhere to these specifications. >>> Unless you do a well documented validation study that goes outside of these restrictions from the IFU that proves no patient harm, you honestly must go by the IFU recommendations. This would apply to all of our stains/reagents/solutions/antibodies. >>> Our world is becoming more restrictive?? >>> >>> hope this helps, >>> Lynette Pavelich, HT(ASCP), QIHC >>> >>> >>>>> On Jul 22, 2020, at 1:55 PM, Hobbs, Carl via Histonet wrote: >>>> >>>> Depends on what you are doing with the sections. >>>> IHC or just dye -staining? >>>> Sure...too hot ( cooking) is not recommended, as stated >>>> Also stated is that high -temp fixation may also be used with no deleterious effects as long as the fixation time is not extended. >>>> However, RT -ish even for a week won't be a problem...imho >>>> Needs must? >>>> >>>> >>>> >>>> Carl Hobbs FIBMS >>>> Histology and Imaging Manager >>>> Wolfson CARD >>>> Guys Campus, London Bridge >>>> Kings College London >>>> London >>>> SE1 1UL >>>> >>>> >>>> 020 7848 6813 >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet at lists.utsouthwestern.edu >>>> https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C2915a105cfa5436de65e08d82e6bd68b%7C8370cf1416f34c16b83c724071654356%7C0&sdata=YZGiBCOwM5NmjBuxHh%2B%2FuhltOh%2F0HxJs5Kb8FaurKQM%3D&reserved=0 >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DThomas at thomasderm.com Wed Jul 22 21:39:05 2020 From: DThomas at thomasderm.com (Douglas Thomas) Date: Thu, 23 Jul 2020 02:39:05 +0000 Subject: [Histonet] New Dermatopath lab in Las Vegas Message-ID: <98717efe43694745a2c80df3c8697b25@TD-V-EX.thomasderm.local> Hi, My name is Doug Thomas MD of Thomas Dermatology in Las Vegas, NV. We are in the process of building a dermatopathology lab. We are looking to hire a couple of histology technicians and possibly a laboratory assistant. Please email me if you are interested at dthomas at thomasderm.com. Thank you, dt From relia1 at earthlink.net Thu Jul 23 11:04:04 2020 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 23 Jul 2020 12:04:04 -0400 Subject: [Histonet] ICYMI My latest article -Fixation on Histology-Maximizing your Career Potential During the COVID-19 Pandemic and New Job Opportunities!! Message-ID: <000001d6610a$e41b7420$ac525c60$@earthlink.net> Hi Histopeeps, ICYMI - In case you missed it!! I am so excited!! My latest article has been published in the NSH Quarterly Career Center Newsletter under my byline: Make the Cut My latest article is entitled: Maximizing your career potential during the COVID-19 pandemic. This is the link to my article on the NSH?s Fixation on Histology Blog and it is also in the current edition of the NSH Career Newsletter. Here is the link to the article: https://www.fixationonhistology.com/post/maximizing-your-career-potential-du ring-the-covid-19-pandemic If you have a minute to read it I would love to hear what you think. If you can?t get to the article with this link let me know and I will send you a copy of it. Histopeeps, we also have amazing opportunities nationwide! 1. N. Carolina Greensboro - Histology Manager 2. N. Carolina Wilmington - Histology Tech 3. N. Carolina Wilmington - Mohs Tech 4. California Central CA - IHC Specialist 5. California Central CA - Histotech 6. California Northern CA - Histology tech 7. Georgia Atlanta ? Mohs Tech 8. S. Carolina Greenville ? Histology Tech 9. Florida Panhandle ? Histo tech 10. Florida Sarasota area - Histotech Please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral reward! My clients offer competitive pay rates, excellent benefits and in most cases Relocation assistance or a sign on bonus. They are ready to interview and hire!!! If You or Anyone You Know Might Be Interested In a New Opportunity, Please Contact Me ASAP If you want to chat ASAP call or text me on my cell At 407-353-5070. If you want some additional information or to set up a time to chat please call me toll free at 866-607-3542 or email me at relia1 at earthlink.net My clients are ready to interview and hire right away!!! And my phone is still ringing off the hook so if you don?t see the location you want yet drop me a line so I can let you know when something DOES come up. I could be talking to a client about your next opportunity RIGHT NOW!! Have a great day. I look forward to hearing back from you. Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From chak_bou at yahoo.com Mon Jul 27 07:31:30 2020 From: chak_bou at yahoo.com (Chakib Boussahmain) Date: Mon, 27 Jul 2020 12:31:30 +0000 (UTC) Subject: [Histonet] Human Tissue Blocks References: <147597083.5365618.1595853090439.ref@mail.yahoo.com> Message-ID: <147597083.5365618.1595853090439@mail.yahoo.com> Dear histoneters,I am working on some immunofluorescence multiplex staining/ optimization of the different kind of antibodies and I am wondering if someone can help me find human tissue block suppliers. I will need different type of tissue normal/disease and different organs.Any advice/ help you guys would give me would be much appreciated.Thank youChakib BoussahmainHistotechnologistCambridge MA From Katherine.Bummer at tevapharm.com Mon Jul 27 09:46:00 2020 From: Katherine.Bummer at tevapharm.com (Katherine Bummer) Date: Mon, 27 Jul 2020 14:46:00 +0000 Subject: [Histonet] Giemsa stain kit recommendations? Message-ID: <5ab5d6ab5a7c4e198b42d02bad5fbce2@USEXCAPP23.Teva.Corp> Hello Histonetters, Does anyone have a Giemsa stain they can recommend? I prefer a kit and I will be staining for neutrophils from a frozen cell pellet? Thank you! Kate This message is intended solely for the designated recipient(s). It may contain confidential or proprietary information and may be subject to attorney-client privilege or other confidentiality protections. If you are not a designated recipient you may not review, copy or distribute this message. If you receive this in error, please notify the sender by reply e-mail and delete this message. Thank you. From erin.mccarthy at tempus.com Mon Jul 27 10:49:49 2020 From: erin.mccarthy at tempus.com (Erin McCarthy) Date: Mon, 27 Jul 2020 10:49:49 -0500 Subject: [Histonet] Human Tissue Blocks In-Reply-To: <147597083.5365618.1595853090439@mail.yahoo.com> References: <147597083.5365618.1595853090439.ref@mail.yahoo.com> <147597083.5365618.1595853090439@mail.yahoo.com> Message-ID: Hi Chakib, I am not sure what kind of budget you have for control tissues, I work in the private sector unaffiliated with any one medical center so we routinely have to buy tissue for control blocks and validations of new antibodies/assays. We use two different suppliers - RS Diagnostics and Cooperative Human Tissue Network (CHTN ) They can be pricey, but that may be due to our business structure and lack of signed agreements to commit to a specific quantity of samples over time. I hope this helps! On Mon, Jul 27, 2020 at 7:48 AM Chakib Boussahmain via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Dear histoneters,I am working on some immunofluorescence multiplex > staining/ optimization of the different kind of antibodies and I am > wondering if someone can help me find human tissue block suppliers. I will > need different type of tissue normal/disease and different organs.Any > advice/ help you guys would give me would be much appreciated.Thank > youChakib BoussahmainHistotechnologistCambridge MA > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Erin McCarthy, HT (ASCP) Histology Supervisor Tempus Labs 600 W. Chicago Ave. Chicago IL 60654 Cell: (708)269-8610 -- This email and any attachments may contain confidential information. If you believe that you have received it in error, please delete the email and attachments and then notify Tempus by calling *800.739.4137*. From edmartin26 at gmail.com Mon Jul 27 13:56:17 2020 From: edmartin26 at gmail.com (Eddie Martin) Date: Mon, 27 Jul 2020 14:56:17 -0400 Subject: [Histonet] Recommend Harleco Giemsa stain reagents In-Reply-To: <5ab5d6ab5a7c4e198b42d02bad5fbce2@USEXCAPP23.Teva.Corp> References: <5ab5d6ab5a7c4e198b42d02bad5fbce2@USEXCAPP23.Teva.Corp> Message-ID: <1FD781C7-0FDD-4675-BA4A-D82CD050E96A@gmail.com> Milliliter sigma makes a brand called harleco for their giemsa?s line. I don?t think they make a kit. But you will need an 1. neutral buffer reagent (harleco) 2.giemsa reagent 3. Azure B reagent And anhydrous methanol solution call me when you get these ans I can provide a working solution for your line up. V/r, Eddie Martin Hematology Team Leader NIH/CC/DLM Hematology Service 301-594-2054 Sent from my iPhone > On Jul 27, 2020, at 10:46 AM, Katherine Bummer wrote: > > ?Hello Histonetters, > > Does anyone have a Giemsa stain they can recommend? I prefer a kit and I will be staining for neutrophils from a frozen cell pellet? > > Thank you! > > Kate > > > This message is intended solely for the designated recipient(s). It may contain confidential or proprietary information and may be subject to attorney-client privilege or other confidentiality protections. If you are not a designated recipient you may not review, copy or distribute this message. If you receive this in error, please notify the sender by reply e-mail and delete this message. Thank you. > From kdean70 at hotmail.com Tue Jul 28 13:43:14 2020 From: kdean70 at hotmail.com (Ken M) Date: Tue, 28 Jul 2020 18:43:14 +0000 Subject: [Histonet] Apple Green Birefringence in Amyliod slides Message-ID: Hi everyone. I was wondering if anyone out there has any experience with diagnosing Amyloid tissue using Congo Red stained Kidney using polarized lenses. Is it common to use polarized light to detect Amyloid deposits? Does the absence of the "apple green birefringence" indicate a problem with the control tissue or the control slides? Should this green bifringence always appear to confirm the diagnosis? I know that the tissue should be cut thicker than normal (we usually cut at 5), but in the future maybe we will cut at 7 or 8? From Timothy.Morken at ucsf.edu Tue Jul 28 13:57:06 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 28 Jul 2020 18:57:06 +0000 Subject: [Histonet] Apple Green Birefringence in Amyliod slides In-Reply-To: References: Message-ID: Ken, Yes, polarized light and apple green birefringence is diagnostic for amyloid with congo red and is the best practice. If you have a problem with known control slides there are two possibilities: 1) make up fresh solution. The pH has to be right. Or 2) try other control slides. Maybe you cut through the amyloid area. Because we have hundreds of microscopes in our department most just use polarized film as the polarizer (put over the light source) and another put over the top of the slide as the analyzer. Turn one of the polarizing slides and you will see the birefringence appear. Source: "Polarizing film, 2"" x 2"" , PK/10 (BEST For use as a microscope polarizer)" Cat# S07372 Thermo Fisher Sci Health $36.75 PK/10 "2" x 2" These are polarized film mounted in 2" film holders (like the old Kodachrome slides). Cheap and effective. (and avoids consternation from people losing expensive microscope polarizers) Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Ken M via Histonet Sent: Tuesday, July 28, 2020 11:43 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Apple Green Birefringence in Amyliod slides Hi everyone. I was wondering if anyone out there has any experience with diagnosing Amyloid tissue using Congo Red stained Kidney using polarized lenses. Is it common to use polarized light to detect Amyloid deposits? Does the absence of the "apple green birefringence" indicate a problem with the control tissue or the control slides? Should this green bifringence always appear to confirm the diagnosis? I know that the tissue should be cut thicker than normal (we usually cut at 5), but in the future maybe we will cut at 7 or 8? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=2jEHe8Hf3ieiSbv1r-ZtSy-mm4FVj1XtTmUSGcfJmE8&s=Q_PpmT_KF2fDUvt9ltFVZLp6ctjM3xkK0RsfuUYW73c&e= From Timothy.Morken at ucsf.edu Tue Jul 28 14:02:19 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 28 Jul 2020 19:02:19 +0000 Subject: [Histonet] Apple Green Birefringence in Amyliod slides In-Reply-To: References: Message-ID: I neglected to mention sections should be cut at 10um for best results. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Morken, Timothy via Histonet Sent: Tuesday, July 28, 2020 11:57 AM To: Ken M Cc: Histonet Subject: Re: [Histonet] Apple Green Birefringence in Amyliod slides Ken, Yes, polarized light and apple green birefringence is diagnostic for amyloid with congo red and is the best practice. If you have a problem with known control slides there are two possibilities: 1) make up fresh solution. The pH has to be right. Or 2) try other control slides. Maybe you cut through the amyloid area. Because we have hundreds of microscopes in our department most just use polarized film as the polarizer (put over the light source) and another put over the top of the slide as the analyzer. Turn one of the polarizing slides and you will see the birefringence appear. Source: "Polarizing film, 2"" x 2"" , PK/10 (BEST For use as a microscope polarizer)" Cat# S07372 Thermo Fisher Sci Health $36.75 PK/10 "2" x 2" These are polarized film mounted in 2" film holders (like the old Kodachrome slides). Cheap and effective. (and avoids consternation from people losing expensive microscope polarizers) Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Ken M via Histonet Sent: Tuesday, July 28, 2020 11:43 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Apple Green Birefringence in Amyliod slides Hi everyone. I was wondering if anyone out there has any experience with diagnosing Amyloid tissue using Congo Red stained Kidney using polarized lenses. Is it common to use polarized light to detect Amyloid deposits? Does the absence of the "apple green birefringence" indicate a problem with the control tissue or the control slides? Should this green bifringence always appear to confirm the diagnosis? I know that the tissue should be cut thicker than normal (we usually cut at 5), but in the future maybe we will cut at 7 or 8? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=2jEHe8Hf3ieiSbv1r-ZtSy-mm4FVj1XtTmUSGcfJmE8&s=Q_PpmT_KF2fDUvt9ltFVZLp6ctjM3xkK0RsfuUYW73c&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=oqbw0CqyFQtZ-sA1KdA3LJ03sCqCrnmvdp3OwwX6wNM&s=60acNUmFhia7wnxpRNPpNBpiz35pC5Rd-Xullc7MbNk&e= From baschainker at gmail.com Tue Jul 28 14:32:50 2020 From: baschainker at gmail.com (Bruce Schainker) Date: Tue, 28 Jul 2020 15:32:50 -0400 Subject: [Histonet] Apple Green Birefringence in Amyliod slides In-Reply-To: References: Message-ID: I would add three additional pointers: 1) Assess in a darkened room with eyes acclimated to the dark; 2) Use a powerful light source--the apple green birefringence will be more obvious and easier to discriminate from the white of collegen; and 3) Assessment sensitivity is further increased if instead of using the inexpensive films that Tim describes you use a more traditional polarizing microscope or a regular light microscope with an polarized lens above the objectives (my microscope has a slide-in slot) and another above the field lens (I use a removable circular polarizer that fits over the entire field lens). Bruce On Tue, Jul 28, 2020 at 3:06 PM Morken, Timothy via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I neglected to mention sections should be cut at 10um for best results. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > -----Original Message----- > From: Morken, Timothy via Histonet > Sent: Tuesday, July 28, 2020 11:57 AM > To: Ken M > Cc: Histonet > Subject: Re: [Histonet] Apple Green Birefringence in Amyliod slides > > Ken, Yes, polarized light and apple green birefringence is diagnostic for > amyloid with congo red and is the best practice. If you have a problem with > known control slides there are two possibilities: 1) make up fresh > solution. The pH has to be right. Or 2) try other control slides. Maybe you > cut through the amyloid area. > > Because we have hundreds of microscopes in our department most just use > polarized film as the polarizer (put over the light source) and another put > over the top of the slide as the analyzer. Turn one of the polarizing > slides and you will see the birefringence appear. > > Source: > "Polarizing film, 2"" x 2"" , PK/10 (BEST For use as a microscope > polarizer)" Cat# S07372 Thermo Fisher Sci Health $36.75 > PK/10 "2" x 2" > > These are polarized film mounted in 2" film holders (like the old > Kodachrome slides). > > Cheap and effective. (and avoids consternation from people losing > expensive microscope polarizers) > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies Department > of Pathology UC San Francisco Medical Center > > > -----Original Message----- > From: Ken M via Histonet > Sent: Tuesday, July 28, 2020 11:43 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Apple Green Birefringence in Amyliod slides > > Hi everyone. I was wondering if anyone out there has any experience with > diagnosing Amyloid tissue using Congo Red stained Kidney using polarized > lenses. Is it common to use polarized light to detect Amyloid deposits? > Does the absence of the "apple green birefringence" indicate a problem with > the control tissue or the control slides? Should this green bifringence > always appear to confirm the diagnosis? I know that the tissue should be > cut thicker than normal (we usually cut at 5), but in the future maybe we > will cut at 7 or 8? > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=2jEHe8Hf3ieiSbv1r-ZtSy-mm4FVj1XtTmUSGcfJmE8&s=Q_PpmT_KF2fDUvt9ltFVZLp6ctjM3xkK0RsfuUYW73c&e= > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=oqbw0CqyFQtZ-sA1KdA3LJ03sCqCrnmvdp3OwwX6wNM&s=60acNUmFhia7wnxpRNPpNBpiz35pC5Rd-Xullc7MbNk&e= > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From john.garratt at ciqc.ca Tue Jul 28 15:00:20 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Tue, 28 Jul 2020 20:00:20 +0000 Subject: [Histonet] Apple Green Birefringence in Amyliod slides In-Reply-To: References: Message-ID: <428VObdXNMn_RRaDDyUUvc6q8G24ywuZRxcgAty0xfvJTjMopEO9FXx-q-4CJZjF3FJOypoY88MZF7qHYJZ-OPlckj7xp2fk0w1ShIQWo7g=@ciqc.ca> Here are a couple of short videos that might be of interest. - John https://www.bing.com/videos/search?q=amyloid+birefringence&&view=detail&mid=0663C1FAAFFA319FD8EA0663C1FAAFFA319FD8EA&&FORM=VRDGAR&ru=%2Fvideos%2Fsearch%3Fq%3Damyloid%2Bbirefringence%26FORM%3DHDRSC3 https://www.bing.com/videos/search?q=amyloid+birefringence&ru=%2fvideos%2fsearch%3fq%3damyloid%2bbirefringence%26FORM%3dHDRSC3&view=detail&mid=25C492C77CCC4B969E6625C492C77CCC4B969E66&&FORM=VDRVRV www.cpqa.ca ??????? Original Message ??????? On Tuesday, July 28, 2020 11:43 AM, Ken M via Histonet wrote: > Hi everyone. I was wondering if anyone out there has any experience with diagnosing Amyloid tissue using Congo Red stained Kidney using polarized lenses. Is it common to use polarized light to detect Amyloid deposits? Does the absence of the "apple green birefringence" indicate a problem with the control tissue or the control slides? Should this green bifringence always appear to confirm the diagnosis? I know that the tissue should be cut thicker than normal (we usually cut at 5), but in the future maybe we will cut at 7 or 8? > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan at uwo.ca Wed Jul 29 13:39:31 2020 From: jkiernan at uwo.ca (John Kiernan) Date: Wed, 29 Jul 2020 18:39:31 +0000 Subject: [Histonet] Apple Green Birefringence in Amyliod slides In-Reply-To: References: , Message-ID: For another source of polarizing filters, go to a 3D movie, take home the glasses they provide, and poke out the lenses. They work very nicely as polarizer and analyzer with an ordinary microscope. John Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada = = = ________________________________ From: Morken, Timothy via Histonet Sent: 28 July 2020 13:57 To: Ken M Cc: Histonet Subject: Re: [Histonet] Apple Green Birefringence in Amyliod slides Ken, Yes, polarized light and apple green birefringence is diagnostic for amyloid with congo red and is the best practice. If you have a problem with known control slides there are two possibilities: 1) make up fresh solution. The pH has to be right. Or 2) try other control slides. Maybe you cut through the amyloid area. Because we have hundreds of microscopes in our department most just use polarized film as the polarizer (put over the light source) and another put over the top of the slide as the analyzer. Turn one of the polarizing slides and you will see the birefringence appear. Source: "Polarizing film, 2"" x 2"" , PK/10 (BEST For use as a microscope polarizer)" Cat# S07372 Thermo Fisher Sci Health $36.75 PK/10 "2" x 2" These are polarized film mounted in 2" film holders (like the old Kodachrome slides). Cheap and effective. (and avoids consternation from people losing expensive microscope polarizers) Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Ken M via Histonet Sent: Tuesday, July 28, 2020 11:43 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Apple Green Birefringence in Amyliod slides Hi everyone. I was wondering if anyone out there has any experience with diagnosing Amyloid tissue using Congo Red stained Kidney using polarized lenses. Is it common to use polarized light to detect Amyloid deposits? Does the absence of the "apple green birefringence" indicate a problem with the control tissue or the control slides? Should this green bifringence always appear to confirm the diagnosis? I know that the tissue should be cut thicker than normal (we usually cut at 5), but in the future maybe we will cut at 7 or 8? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=2jEHe8Hf3ieiSbv1r-ZtSy-mm4FVj1XtTmUSGcfJmE8&s=Q_PpmT_KF2fDUvt9ltFVZLp6ctjM3xkK0RsfuUYW73c&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robrankin at rankinbiomed.com Thu Jul 30 09:26:57 2020 From: robrankin at rankinbiomed.com (Rob Rankin) Date: Thu, 30 Jul 2020 10:26:57 -0400 Subject: [Histonet] Sakura Tissue-Tek versus Tanner embedding station Comparison Message-ID: Is anyone using a Tanner embedding station? If so, what has your experience been like? I'm trying to compare between a Sakura Tissue-Tek and Tanner (the Tanner is much cheaper). Respectfully, *Rob Rankin, MSM, SM(ASCP)* Founder & CEO Office: 248 625-4104 ext 0012 www.rankinbiomed.com 14515 Mackey Rd. Holly, MI 48442 *Have not I commanded thee? Be strong and of good courage; be not afraid, neither be thou dismayed: for the LORD thy God is with thee whithersoever thou goest. * *Joshua 1:9* From madeleinehuey at gmail.com Thu Jul 30 13:06:25 2020 From: madeleinehuey at gmail.com (Madeleine Huey) Date: Thu, 30 Jul 2020 11:06:25 -0700 Subject: [Histonet] Histonet Digest, Vol 200, Issue 19 In-Reply-To: References: Message-ID: Original Message----- From: Ken M via Histonet Sent: Tuesday, July 28, 2020 11:43 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Apple Green Birefringence in Amyliod slides Hi Ken, May I give you my personal experience on Congo Red? 1) Always cut thick tissue (ie,10um thickness, but 8 um will work) 2) Always fresh cut tissue. You can pre-cut control slides, but store them in the *dark* @ *4C (ie. fridge, never RT)*. Amyloid will fade if pre-cut and stored at room temperature with light. We always stored our stained control slide as reference along with our pre-cut unstained slides in the fridge, but "apple green birefringence" will also fade with time (unstable). *Madeleine Huey, **B.S., HTL & QIHC (ASCP)* Supervisor, Pathology/Histology & IPOX (MV & LG) El Camino Hospital 2500 Grant Road (Rm GC-33) Mountain View, CA 94040 Phone: 650-940-7038 Fax: 650-988-8387 Email: madeleine_h at elcaminohospital.org Hi everyone. I was wondering if anyone out there has any experience with diagnosing Amyloid tissue using Congo Red stained Kidney using polarized lenses. Is it common to use polarized light to detect Amyloid deposits? Does the absence of the "apple green birefringence" indicate a problem with the control tissue or the control slides? Should this green bifringence always appear to confirm the diagnosis? I know that the tissue should be cut thicker than normal (we usually cut at 5), but in the future maybe we will cut at 7 or 8? On Thu, Jul 30, 2020 at 10:04 AM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Apple Green Birefringence in Amyliod slides (John Kiernan) > 2. Sakura Tissue-Tek versus Tanner embedding station Comparison > (Rob Rankin) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 29 Jul 2020 18:39:31 +0000 > From: John Kiernan > To: Ken M , "Morken, Timothy" > > Cc: Histonet > Subject: Re: [Histonet] Apple Green Birefringence in Amyliod slides > Message-ID: > < > YTOPR0101MB161161FC59817A6B556CCDEFA5700 at YTOPR0101MB1611.CANPRD01.PROD.OUTLOOK.COM > > > > Content-Type: text/plain; charset="iso-8859-1" > > For another source of polarizing filters, go to a 3D movie, take home the > glasses they provide, and poke out the lenses. They work very nicely as > polarizer and analyzer with an ordinary microscope. > John Kiernan > Anatomy & Cell Biology > University of Western Ontario > London, Canada > = = = > ________________________________ > From: Morken, Timothy via Histonet > Sent: 28 July 2020 13:57 > To: Ken M > Cc: Histonet > Subject: Re: [Histonet] Apple Green Birefringence in Amyliod slides > > Ken, Yes, polarized light and apple green birefringence is diagnostic for > amyloid with congo red and is the best practice. If you have a problem with > known control slides there are two possibilities: 1) make up fresh > solution. The pH has to be right. Or 2) try other control slides. Maybe you > cut through the amyloid area. > > Because we have hundreds of microscopes in our department most just use > polarized film as the polarizer (put over the light source) and another put > over the top of the slide as the analyzer. Turn one of the polarizing > slides and you will see the birefringence appear. > > Source: > "Polarizing film, 2"" x 2"" , PK/10 (BEST For use as a microscope > polarizer)" Cat# S07372 Thermo Fisher Sci Health $36.75 > PK/10 "2" x 2" > > These are polarized film mounted in 2" film holders (like the old > Kodachrome slides). > > Cheap and effective. (and avoids consternation from people losing > expensive microscope polarizers) > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > -----Original Message----- > From: Ken M via Histonet > Sent: Tuesday, July 28, 2020 11:43 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Apple Green Birefringence in Amyliod slides > > Hi everyone. I was wondering if anyone out there has any experience with > diagnosing Amyloid tissue using Congo Red stained Kidney using polarized > lenses. Is it common to use polarized light to detect Amyloid deposits? > Does the absence of the "apple green birefringence" indicate a problem with > the control tissue or the control slides? Should this green bifringence > always appear to confirm the diagnosis? I know that the tissue should be > cut thicker than normal (we usually cut at 5), but in the future maybe we > will cut at 7 or 8? > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=2jEHe8Hf3ieiSbv1r-ZtSy-mm4FVj1XtTmUSGcfJmE8&s=Q_PpmT_KF2fDUvt9ltFVZLp6ctjM3xkK0RsfuUYW73c&e= > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 2 > Date: Thu, 30 Jul 2020 10:26:57 -0400 > From: Rob Rankin > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Sakura Tissue-Tek versus Tanner embedding station > Comparison > Message-ID: > Wmhh-Kh51DVHSt8Cg at mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Is anyone using a Tanner embedding station? If so, what has your experience > been like? I'm trying to compare between a Sakura Tissue-Tek and Tanner > (the Tanner is much cheaper). > > Respectfully, > *Rob Rankin, MSM, SM(ASCP)* > Founder & CEO > Office: 248 625-4104 ext 0012 > www.rankinbiomed.com > > > > 14515 Mackey Rd. > > Holly, MI 48442 > > > > > > *Have not I commanded thee? Be strong and of good courage; be not > afraid, neither be thou dismayed: for the LORD thy God is with thee > whithersoever thou goest. * > > *Joshua 1:9* > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 200, Issue 19 > ***************************************** > From carl.hobbs at kcl.ac.uk Fri Jul 31 12:38:42 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 31 Jul 2020 17:38:42 +0000 Subject: [Histonet] Apple Green Birefringence in Amyliod slides Message-ID: I have images on Histonet image archive Congo red brightfield and also fluorescence using the TRITC channel. No green birefringence images tho I find fluorescence more sensitive for Congo red However, my std is to use an anti Amyloid beta antibody. If anyone cares to look and comment.....I'm always listening/reading.... to improve my practise/practice ( which is the korrekt word?....chuckle) Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813