From carlos.torresvega at gmail.com Wed Jan 1 19:58:30 2020 From: carlos.torresvega at gmail.com (Carlos Torres Vega) Date: Wed, 1 Jan 2020 19:58:30 -0600 Subject: [Histonet] asking for Leica ST 5050 manual Message-ID: Does somebody have an operation manual or service manual (great) for a Leica ST 5050 Immunosteiner. I will appreciate your help if somebody can share it wit me. thanks a have a wonderful new year ! From jkiernan at uwo.ca Thu Jan 2 00:17:32 2020 From: jkiernan at uwo.ca (John Kiernan) Date: Thu, 2 Jan 2020 06:17:32 +0000 Subject: [Histonet] Pap stains In-Reply-To: <5135B39B-B5D1-42A8-ADD0-9224273D34FC@slh.wisc.edu> References: , <5135B39B-B5D1-42A8-ADD0-9224273D34FC@slh.wisc.edu> Message-ID: As a student in the 1960s I was told by my elders and betters to filter all staining solutions before using. It was very good advice. Filtration does not prolong the life of a stain, but it does remove crud, a material that can be composed of polymerized dyes and bits of previously stained cells or tissues. Fifty years on, I can identify a few staining solutions that never deteriorate into insoluble materials that fall out of solution even after 10+ years. Even for these, filtration before use is good laboratory practice. Not filtering any stain may therefore be bad laboratory practice. Happy New Year, John Kiernan = = = ________________________________ From: Haas, Elizabeth via Histonet Sent: 31 December 2019 11:46 To: S hay Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pap stains I believe filtering stains daily is a CAP requirement Sent from my iPhone > On Dec 31, 2019, at 9:23 AM, S hay via Histonet wrote: > > ?1. Does everyone filter their pap stains daily? > 2. Are you chaining all other reagents daily? > > Thanks in advance. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ LEGAL DISCLAIMER: This message and all attachments may be confidential or protected by privilege. If you are not the intended recipient you are hereby notified that any disclosure, copying, distribution, or use of the information contained in or attached to this message is strictly prohibited. Please notify the sender of the delivery error by replying to this message and then delete it from your system. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From susanhay59 at gmail.com Thu Jan 2 06:55:30 2020 From: susanhay59 at gmail.com (S hay) Date: Thu, 2 Jan 2020 06:55:30 -0600 Subject: [Histonet] Pap stains In-Reply-To: References: <5135B39B-B5D1-42A8-ADD0-9224273D34FC@slh.wisc.edu> Message-ID: Hi John but what about ALL of the other solutions in a staining row that could be potentially contaminated. Why are folks only filtering the dyes? On Thu, Jan 2, 2020, 12:17 AM John Kiernan wrote: > As a student in the 1960s I was told by my elders and betters to filter > all staining solutions before using. It was very good advice. Filtration > does not prolong the life of a stain, but it does remove *crud*, a > material that can be composed of polymerized dyes and bits of previously > stained cells or tissues. > > Fifty years on, I can identify a few staining solutions that never > deteriorate into insoluble materials that fall out of solution even after > 10+ years. Even for these, filtration before use is good laboratory > practice. Not filtering any stain may therefore be bad laboratory practice. > > Happy New Year, * John Kiernan* > = = = > ------------------------------ > *From:* Haas, Elizabeth via Histonet > *Sent:* 31 December 2019 11:46 > *To:* S hay > *Cc:* histonet at lists.utsouthwestern.edu > > *Subject:* Re: [Histonet] Pap stains > > > I believe filtering stains daily is a CAP requirement > > > Sent from my iPhone > > > On Dec 31, 2019, at 9:23 AM, S hay via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > > ?1. Does everyone filter their pap stains daily? > > 2. Are you chaining all other reagents daily? > > > > Thanks in advance. > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ________________________________ > > > LEGAL DISCLAIMER: This message and all attachments may be confidential or > protected by privilege. If you are not the intended recipient you are > hereby notified that any disclosure, copying, distribution, or use of the > information contained in or attached to this message is strictly > prohibited. Please notify the sender of the delivery error by replying to > this message and then delete it from your system. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From LRaff at uropartners.com Thu Jan 2 08:00:22 2020 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 2 Jan 2020 14:00:22 +0000 Subject: [Histonet] A New Look at the Lab--Happy New Year! Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF128096DD@COLOEXCH01.uropartners.local> http://www.chicagonow.com/downsize-maybe/2020/01/timeline/ Lester J. Raff, MD MBA FCAP Laboratory Director UroPartners LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 Telephone 708-486-0076 Fax 708-492-0203 From criley at dpspa.com Thu Jan 2 08:51:21 2020 From: criley at dpspa.com (Charles Riley) Date: Thu, 2 Jan 2020 09:51:21 -0500 Subject: [Histonet] Nanticoke Memorial in Seaford DE Message-ID: I am trying to reach anyone who works in the histo lab at Nanticoke. If you do and happen to see this can you please reply back. I am trying to reach out about your H&E staining process -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From relia1 at earthlink.net Thu Jan 2 09:50:35 2020 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 2 Jan 2020 10:50:35 -0500 Subject: [Histonet] OT: Happy New Year Histopeeps!!! A Histology Matching Game to start 2020 with a smile! Message-ID: <000001d5c184$5f5afce0$1e10f6a0$@earthlink.net> Hi Histopeeps! Happy New Year!!!! Here?s to a terrific 2020!!!!!!! How did you ring in the New Year? I hope you had fun ushering out 2019 and welcoming in 2020. I know I am looking forward to an exciting new year. Here is something to get your New Year started off with a SMILE! It?s a Histology Matching Game. Check it out: https://matchthememory.com/HistologyMatch I want to let you know that I have been chatting with clients and have Some Exciting New Opportunities. I also have some new features added to my weekly bulletin that aren't going to be posted on histonet. Don't miss out! if you would like to subscribe just reply to this post with I'm IN! and be sure and give me the email address you want to receive this bulletin at. Happy New Year to You & Yours!! I wish you a year of love, laughter, good health & prosperity. Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From criley at dpspa.com Fri Jan 3 11:42:40 2020 From: criley at dpspa.com (Charles Riley) Date: Fri, 3 Jan 2020 12:42:40 -0500 Subject: [Histonet] H&E protocols Message-ID: Does everyone use the same protocol for routine H&E's as well as things like Cell blocks and or lymph nodes? If you use a different protocol how does it differ from your routine stain? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From tbraud at holyredeemer.com Fri Jan 3 12:22:19 2020 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 3 Jan 2020 18:22:19 +0000 Subject: [Histonet] H&E protocols Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B6EA3E@HRHEX02-HOS.holyredeemer.local> We use the same protocol for all H&Es Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Sent: Friday, January 03, 2020 1:00 PM Subject: Histonet Digest, Vol 194, Issue 2 Today's Topics: 1. H&E protocols (Charles Riley) Date: Fri, 3 Jan 2020 12:42:40 -0500 From: Charles Riley Does everyone use the same protocol for routine H&E's as well as things like Cell blocks and or lymph nodes? If you use a different protocol how does it differ from your routine stain? Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From Dawn.Olszewski at SGMC.ORG Fri Jan 3 13:43:05 2020 From: Dawn.Olszewski at SGMC.ORG (Olszewski, Dawn) Date: Fri, 3 Jan 2020 19:43:05 +0000 Subject: [Histonet] Histonet Digest, Vol 194, Issue 2 In-Reply-To: References: <12a3486f-aba0-466f-9fef-6a4ca11e0a30.3b5c5640-2195-4f35-b5ef-5e1ebee68512.4551b4f3-c8ed-4c2e-9dbd-951cdb6760f9@emailsignatures365.codetwo.com> Message-ID: Charles, We use the same protocol but we also cut everything at 5 microns. Dawn Olszewski HTL(ASCP)QIHC Pathology Manager South Georgia Medical Center P: (229) 259-4830 E: dawn.olszewski at sgmc.org Dawn Olszewski Pathology Manager - Histotechnologist Laboratory [cid:MasterLogo_190x61_362365a5-c941-4f16-9f99-9484fd6a6078.png] South Georgia Medical Center 2501 N. Patterson St. Valdosta, GA 31602 229-259-4830 (O) | 229-560-6191 (M) Dawn.Olszewski at SGMC.ORG | sgmc.org [cid:facebook_fb_32x32_0a6d9065-09e0-48f7-88c4-b2d459a5e547.png] [cid:twitter_32x32_5a3a5eeb-77c7-4a3c-aa09-b69b41f32bd2.png] [cid:linkedin_ln_32x32_bf1e5dc9-b886-480a-8c8b-ef8a1ae87168.png] [cid:youtube_play_32x32_572cd3ed-390b-48e0-8845-ba1f81953de3.png] ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Friday, January 3, 2020 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 194, Issue 2 WARNING: This email originated outside the SGMC?s email system. DO NOT CLICK: Links or attachments unless you recognize the sender and know the content is safe. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7CDawn.Olszewski%40sgmc.org%7C808461016dfa4bcba59708d79076e6ab%7C90cc13a5f2044538be2d7055be683ef1%7C0&sdata=wxxn88bBTFVLmItfsqz74MmP2H3idmaiGSYM4GzhtLc%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. H&E protocols (Charles Riley) ---------------------------------------------------------------------- Message: 1 Date: Fri, 3 Jan 2020 12:42:40 -0500 From: Charles Riley To: Histo List Subject: [Histonet] H&E protocols Message-ID: Content-Type: text/plain; charset="UTF-8" Does everyone use the same protocol for routine H&E's as well as things like Cell blocks and or lymph nodes? If you use a different protocol how does it differ from your routine stain? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7CDawn.Olszewski%40sgmc.org%7C808461016dfa4bcba59708d79076e6ab%7C90cc13a5f2044538be2d7055be683ef1%7C0&sdata=wxxn88bBTFVLmItfsqz74MmP2H3idmaiGSYM4GzhtLc%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 194, Issue 2 **************************************** From tony.henwood at health.nsw.gov.au Fri Jan 3 15:34:29 2020 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 3 Jan 2020 21:34:29 +0000 Subject: [Histonet] H&E protocols In-Reply-To: References: Message-ID: <1578087268748.17662@health.nsw.gov.au> Hi Charles, Yes we use the same protocol for all, though sometimes we need to modify if for example tissue has been overly decalcified (eg mandible). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Charles Riley via Histonet Sent: Saturday, 4 January 2020 04:42 To: Histo List Subject: [Histonet] H&E protocols Does everyone use the same protocol for routine H&E's as well as things like Cell blocks and or lymph nodes? If you use a different protocol how does it differ from your routine stain? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From cyang at sterlingpath.com Fri Jan 3 18:59:26 2020 From: cyang at sterlingpath.com (cyang at sterlingpath.com) Date: Sat, 4 Jan 2020 00:59:26 +0000 (UTC) Subject: [Histonet] Seeking Histotech References: <290582504.6536609.1578099566661.ref@mail.yahoo.com> Message-ID: <290582504.6536609.1578099566661@mail.yahoo.com> Sterling Pathology Natl Labs located in Seal Beach, California. We are seeking an experienced histotech. We are doing mostly GI and GU biopsies about 150 a day. Some skin and small surgicals from surgery centers. We use microwave process and 3 Vantana IHC machines. Average 1 bone marrow or blood a day. Please respond to email with resume. My best regards and happy new years ! Changgao Yang, MD From naira.margaryan at hsc.wvu.edu Tue Jan 7 12:21:31 2020 From: naira.margaryan at hsc.wvu.edu (Margaryan, Naira) Date: Tue, 7 Jan 2020 18:21:31 +0000 Subject: [Histonet] X-gal staining Message-ID: Hello histonetters, I have request from a scientist to perform X-gal staining on the formalin fixed paraffin-embedded kidney. I was wondering if there is any possibility to do this staining on FFPE tissue as well as if there any possibility to perform a peroxidase DAB staining for the X-gal? If so, may I ask you for a detailed protocol to perform this staining. Any input and explanation why it is impossible is appreciated. Thanks in advance, Naira From CWaitts at Centrastate.com Wed Jan 8 10:46:18 2020 From: CWaitts at Centrastate.com (Waitts, Celeste) Date: Wed, 8 Jan 2020 16:46:18 +0000 Subject: [Histonet] (no subject) Message-ID: <131bf77daa5d4ba08f9076d9afbf1f65@exchnode1.local.centrastate.com> Question VIP6 SHORT BIOPSY RUN USING CLEARITE ANYTHING Celeste Waitts Histology Supervisor Centrastate Medical Center Freehold, NJ 07728 (732-303-5071) From kimberly.glover at polysciences.com Thu Jan 9 12:39:18 2020 From: kimberly.glover at polysciences.com (Glover, Kimberly) Date: Thu, 9 Jan 2020 18:39:18 +0000 Subject: [Histonet] Need a Slide Scanner Message-ID: Hi, I am interested in purchasing a slide scanner for my lab so we will have the ability to share slides between sites. Can someone suggest a good scanner, make/model? Kimberly Glover Product Manager, Histology and Hematology Warrington, PA From regan.fulton at gmail.com Thu Jan 9 14:04:54 2020 From: regan.fulton at gmail.com (Fulton Regan) Date: Thu, 9 Jan 2020 12:04:54 -0800 Subject: [Histonet] Need a Slide Scanner In-Reply-To: References: Message-ID: <8B2BEB0F-1E75-4FBE-AD2C-BF4C27370BAC@gmail.com> Hi Kimberly, We recently purchased a Grundium Ocus model desktop scanner. They are a new manufacturer from Finland. The instrument scans one slide at a time and has a single objective (20X). Scan time ~2 minutes for 15x15mm. The ease of use for our low-throughput applications is very impressive. The image quality is great. The price point is also very attractive. We are very happy with it, so far. Good luck! Regan Regan Fulton, M.D., Ph.D. CEO and Founder Array Science, LLC 475 Gate 5 Road, #102 Sausalito, CA 94965 www.arrayscience.com > On Jan 9, 2020, at 10:39 AM, Glover, Kimberly via Histonet wrote: > > Hi, > > I am interested in purchasing a slide scanner for my lab so we will have the ability to share slides between sites. Can someone suggest a good scanner, make/model? > > Kimberly Glover > Product Manager, Histology and Hematology > Warrington, PA > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimberly.glover at polysciences.com Thu Jan 9 15:27:32 2020 From: kimberly.glover at polysciences.com (Glover, Kimberly) Date: Thu, 9 Jan 2020 21:27:32 +0000 Subject: [Histonet] FW: Need a Slide Scanner References: <5f32db95a3bf47f0afcfcffe2a1a9e03@chla.usc.edu> <11649bb528c74839bf098ae00a91d7c4@chla.usc.edu> Message-ID: -----Original Message----- From: Glover, Kimberly Sent: Thursday, January 09, 2020 2:14 PM To: 'Cooper, Brian' Subject: RE: [Histonet] Need a Slide Scanner Good information! It will be something exciting for us since it is a new feature in the lab. Hopefully the excitement will linger for many years to come. Thanks again, Kimberly -----Original Message----- From: Cooper, Brian [mailto:bcooper at chla.usc.edu] Sent: Thursday, January 09, 2020 2:07 PM To: Glover, Kimberly Subject: RE: [Histonet] Need a Slide Scanner It's actually been awhile since I did the scanning myself, but I think it was something like 40-50 or so slides an hour at 20X magnification. 40X takes considerably longer. We now have transferred scanning to another section, so I'm rusty for sure. I remember that the system takes a really quick pre-snap, and makes you add focus points, and select areas to scan/exclude. This was kind of tedious, but easy. If you're buying a brand new scanner, I imagine the process has been made soooo much better by now, and the scanning much faster. Thanks, Brian -----Original Message----- From: Glover, Kimberly [mailto:kimberly.glover at polysciences.com] Sent: Thursday, January 09, 2020 10:51 AM To: Cooper, Brian Subject: RE: [Histonet] Need a Slide Scanner (EXTERNAL EMAIL) Hi Brian, Thank you for the recommendation. I will definitely look for this one as it may also be more cost effective since it has been out for a while. How long does it take to scan slides? Kimberly -----Original Message----- From: Cooper, Brian [mailto:bcooper at chla.usc.edu] Sent: Thursday, January 09, 2020 1:45 PM To: Glover, Kimberly Subject: RE: [Histonet] Need a Slide Scanner We have the Leica Aperio AT2 here. Works pretty well. I understand that newer scanners are a whole lot faster than what we have here; ours is about 6-7 years old now. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu -----Original Message----- From: Glover, Kimberly via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, January 09, 2020 10:39 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Need a Slide Scanner (EXTERNAL EMAIL) Hi, I am interested in purchasing a slide scanner for my lab so we will have the ability to share slides between sites. Can someone suggest a good scanner, make/model? Kimberly Glover Product Manager, Histology and Hematology Warrington, PA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. --------------------------------------------------------------- This message was processed through Polysciences, Inc. email security system. Any issues can be reported to the Polysciences Helpdesk via emailing helpdesk at polysciences.com CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From kimberly.glover at polysciences.com Thu Jan 9 16:28:13 2020 From: kimberly.glover at polysciences.com (Glover, Kimberly) Date: Thu, 9 Jan 2020 22:28:13 +0000 Subject: [Histonet] Need a Slide Scanner In-Reply-To: References: Message-ID: Hi Tim, Thank you for your help with my search. It sounds like I will need to use a high resolution camera if there are no options for a combined system or either select 2 systems. Kimberly -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Thursday, January 09, 2020 5:12 PM To: Glover, Kimberly Subject: RE: Need a Slide Scanner No, it won't accommodate that. I'm not sure any will. It would take a long time to scan a slide at that magnification. You may be better off with individual pictures for that. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Glover, Kimberly Sent: Thursday, January 09, 2020 12:40 PM To: Morken, Timothy Cc: hisonet at list.utsouthwestern.edu Subject: RE: Need a Slide Scanner Hi Tim, Will the Mikroscan be good for oil immersion slides also? Kimberly -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Thursday, January 09, 2020 2:32 PM To: Glover, Kimberly Subject: RE: Need a Slide Scanner If you only do small batches the Mikroscan is great. The Philips is very expensive and designed for large batch high-throughput. You only need a scanner where you have the slides to scan. The images go on a server for viewing. We have one at Each gross room because the slides might be viewed by pathologists anywhere in our 3 facilities. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Glover, Kimberly Sent: Thursday, January 09, 2020 11:23 AM To: Morken, Timothy Subject: RE: Need a Slide Scanner Hi Tim, We will send slides out throughout the day for remote diagnosis. Is the Mikroscan L5 better for this? Do we need to have a scanner at both locations? Thanks, Kimberly -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Thursday, January 09, 2020 2:14 PM To: Glover, Kimberly Subject: RE: Need a Slide Scanner Kimberly, all at once, or staggered throughout the day? We have two types of scanners. One is a Philips high capacity that we use to scan all slides and can accommodate many 20-slide racks at once. It also requires a huge amount of server storage space. We have 4 of those with the plan to scan up to 1000 slides per day. It would do your 50 slides in about an 90 minutes total in one batch. We also have small scanner, a Mikroscan L5 that takes up to 6 slides in a tray that we use to scan frozen sections for remote diagnosis. It is good for random use all day in the gross room We have one at each of our three gross rooms. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Glover, Kimberly Sent: Thursday, January 09, 2020 10:55 AM To: Morken, Timothy Subject: RE: Need a Slide Scanner Tim, That is still to be determined. It will start as a small volume, maybe 20-50 slides a day just to collaborate. Kimberly -----Original Message----- From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] Sent: Thursday, January 09, 2020 1:51 PM To: Glover, Kimberly Cc: histonet-request at lists.utsouthwestern.edu Subject: RE: Need a Slide Scanner Kimberly, how many slides will you be scanning daily? Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Glover, Kimberly via Histonet Sent: Thursday, January 09, 2020 10:39 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Need a Slide Scanner Hi, I am interested in purchasing a slide scanner for my lab so we will have the ability to share slides between sites. Can someone suggest a good scanner, make/model? Kimberly Glover Product Manager, Histology and Hematology Warrington, PA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=a1l_72EbDiJpfYsMVR_8DnxWy_3aBtCFkmh3La5_ki4&s=Sf275owePKA4SXbjIkhgbG9vJAi3W06StjqAm7qpsJY&e= --------------------------------------------------------------- This message was processed through Polysciences, Inc. email security system. Any issues can be reported to the Polysciences Helpdesk via emailing helpdesk at polysciences.com From john.garratt at ciqc.ca Thu Jan 9 16:56:03 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 09 Jan 2020 22:56:03 +0000 Subject: [Histonet] Need a Slide Scanner In-Reply-To: References: Message-ID: I suggest contacting Hamamatsu in the US. DAriyakumar at hamamatsu.com John Sent from ProtonMail Mobile On Thu, Jan 9, 2020 at 2:28 PM, Glover, Kimberly via Histonet wrote: > Hi Tim, > > Thank you for your help with my search. It sounds like I will need to use a high resolution camera if there are no options for a combined system or either select 2 systems. > > Kimberly > -----Original Message----- > From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] > Sent: Thursday, January 09, 2020 5:12 PM > To: Glover, Kimberly > Subject: RE: Need a Slide Scanner > > No, it won't accommodate that. I'm not sure any will. It would take a long time to scan a slide at that magnification. You may be better off with individual pictures for that. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > -----Original Message----- > From: Glover, Kimberly > Sent: Thursday, January 09, 2020 12:40 PM > To: Morken, Timothy > Cc: hisonet at list.utsouthwestern.edu > Subject: RE: Need a Slide Scanner > > Hi Tim, > > Will the Mikroscan be good for oil immersion slides also? > > Kimberly > > -----Original Message----- > From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] > Sent: Thursday, January 09, 2020 2:32 PM > To: Glover, Kimberly > Subject: RE: Need a Slide Scanner > > If you only do small batches the Mikroscan is great. The Philips is very expensive and designed for large batch high-throughput. > > You only need a scanner where you have the slides to scan. The images go on a server for viewing. We have one at Each gross room because the slides might be viewed by pathologists anywhere in our 3 facilities. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center > > -----Original Message----- > From: Glover, Kimberly > Sent: Thursday, January 09, 2020 11:23 AM > To: Morken, Timothy > Subject: RE: Need a Slide Scanner > > Hi Tim, > > We will send slides out throughout the day for remote diagnosis. Is the Mikroscan L5 better for this? Do we need to have a scanner at both locations? > > Thanks, > Kimberly > > -----Original Message----- > From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] > Sent: Thursday, January 09, 2020 2:14 PM > To: Glover, Kimberly > Subject: RE: Need a Slide Scanner > > Kimberly, all at once, or staggered throughout the day? > > We have two types of scanners. > > One is a Philips high capacity that we use to scan all slides and can accommodate many 20-slide racks at once. It also requires a huge amount of server storage space. We have 4 of those with the plan to scan up to 1000 slides per day. It would do your 50 slides in about an 90 minutes total in one batch. > > We also have small scanner, a Mikroscan L5 that takes up to 6 slides in a tray that we use to scan frozen sections for remote diagnosis. It is good for random use all day in the gross room We have one at each of our three gross rooms. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center > > -----Original Message----- > From: Glover, Kimberly > Sent: Thursday, January 09, 2020 10:55 AM > To: Morken, Timothy > Subject: RE: Need a Slide Scanner > > Tim, > > That is still to be determined. It will start as a small volume, maybe 20-50 slides a day just to collaborate. > > Kimberly > > -----Original Message----- > From: Morken, Timothy [mailto:Timothy.Morken at ucsf.edu] > Sent: Thursday, January 09, 2020 1:51 PM > To: Glover, Kimberly > Cc: histonet-request at lists.utsouthwestern.edu > Subject: RE: Need a Slide Scanner > > Kimberly, how many slides will you be scanning daily? > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center > > -----Original Message----- > From: Glover, Kimberly via Histonet > Sent: Thursday, January 09, 2020 10:39 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Need a Slide Scanner > > Hi, > > I am interested in purchasing a slide scanner for my lab so we will have the ability to share slides between sites. Can someone suggest a good scanner, make/model? > > Kimberly Glover > Product Manager, Histology and Hematology Warrington, PA > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=a1l_72EbDiJpfYsMVR_8DnxWy_3aBtCFkmh3La5_ki4&s=Sf275owePKA4SXbjIkhgbG9vJAi3W06StjqAm7qpsJY&e= > --------------------------------------------------------------- > This message was processed through Polysciences, Inc. email security system. Any issues can be reported to the Polysciences Helpdesk via emailing helpdesk at polysciences.com > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dorianne.m.bonello at gov.mt Fri Jan 10 03:09:40 2020 From: dorianne.m.bonello at gov.mt (Bonello Dorianne M at Health-MDH) Date: Fri, 10 Jan 2020 09:09:40 +0000 Subject: [Histonet] slippery floors Message-ID: <62442f5c68b5478d858051d3d8e94748@gov.mt> Dear all, we are currently experiencing problems due to our lab/corridor linoleum floors which have become too slippery. Anyone experiencing the same problem? If so, can you please suggest any solutions/measures taken to make the floor less slippery. Regards, Dorianne From michael.gudo at morphisto.de Fri Jan 10 03:59:06 2020 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Fri, 10 Jan 2020 10:59:06 +0100 Subject: [Histonet] Need a Slide Scanner In-Reply-To: References: Message-ID: <7529AB03-0A05-4C6B-8365-8CFE9638CF99@morphisto.de> Dear Kimberly, we have two systems here in our lab, both with microscopes from ZEISS. The first one is a TissueFaxs-System which is for scanning of maximum 8 Slides and special optical requirements (polarization, phase contrast, etc.). This system is quite good and flexible and you can use almost all optical parameters of the microscope, including fluorescense. However, compared to slide scanners its relatively slow. Our system is about 5 or 6 years old, but I know that TissueGnostics developed larger and faster systems working with a complete microscope and a loading system for 100 or 200 slides. If you have larger slides, TissueGnostics also provides slide holder for very larger slides (as far as I remember 15 x 24 cm). Our second system for larger amounts is the ZEISS Axioscan (https://www.morphisto.de/en/service/digital-pathology/), which is in our opinion the best scanning system on the market, since it also works with a complete microscope and a very good Software (ZEN). ZEN looks a bit difficult at the first sight, but it offers many many features to get very very good scanning results. For scanning of slides speed is not the most important parameter, but correct optical representation. Many scanners are just fast, because they close the aperture diaphragm and light-field diaphragm, to generate high contrast, but as everybody knows who is familiar with working on a manual microscope, your produce virtual (fake) structures. With the ZEISS Axioscan you can make Z-stacks, which means that the same slide/region will be scanned several times at different focus layers, so that you can finally go through this stack on the computer. The ZEISS Axioscan starts with a focus map, to save focussing time, and its possible to optimize this focus map for speed or for quality. ZEISS also offers a free ZEN-light version, which can be used on every computer to open and analyse the scanned slides. The ZEISS Axioscan is also able to handle larger slides (50 x 76 mm) and it can be upgraded for fluorescence and polarisation. If you like to see some sample, please contact me, I think we can also make a contact to ZEISS, if you are interested. Kind regards Michael > Am 09.01.2020 um 19:39 schrieb Glover, Kimberly via Histonet : > > Hi, > > I am interested in purchasing a slide scanner for my lab so we will have the ability to share slides between sites. Can someone suggest a good scanner, make/model? > > Kimberly Glover > Product Manager, Histology and Hematology > Warrington, PA > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************** MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From tbraud at holyredeemer.com Fri Jan 10 12:18:21 2020 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 10 Jan 2020 18:18:21 +0000 Subject: [Histonet] Slippery floors Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B70B6A@HRHEX02-HOS.holyredeemer.local> Dear Dorianne - Over 40+ years, I've seen all sorts of attempts at solving the "slippery floor" issue; mats, special flooring, etc. In my experience, there is no substitute for having the Environmental Services folks, strip the floors every 2 weeks. I find that while applications of floor finish (wax)make the floors look nice and shiny, they only serve to exacerbate the slipperiness. Try it, you will like it! Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Message: 6 Date: Fri, 10 Jan 2020 09:09:40 +0000 From: Bonello Dorianne M at Health-MDH Subject: [Histonet] slippery floors Dear all, we are currently experiencing problems due to our lab/corridor linoleum floors which have become too slippery. Anyone experiencing the same problem? If so, can you please suggest any solutions/measures taken to make the floor less slippery. Regards, Dorianne From carl.hobbs at kcl.ac.uk Sat Jan 11 13:20:30 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sat, 11 Jan 2020 19:20:30 +0000 Subject: [Histonet] X-gal staining In-Reply-To: References: Message-ID: Hi No replies so far so.....my pennyworth. Imho ...no beta Gal enzyme is inactivated by std Pwax processing. So, when you apply the X-gal substrate and chromogen solution to Pwax sections, it will not give you a positive blue final reaction product If you want to detect beta Gal in FFPWS and visualise with DAB, you will have to use an anti beta Gal antibody. Finding an anti beta Gal enzyme antibody, that works in FFPWS +/- AR is not so easy. Good luck Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From: histonet-request at lists.utsouthwestern.edu Sent: 08 January 2020 18:00 To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 194, Issue 4 ? Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C5189a7f8df3b4f65b02708d79465e645%7C8370cf1416f34c16b83c724071654356%7C0&sdata=c06fBHXWIVgPOnNXhSw7mEFHHvThteuUFXxa5UYSlCI%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. X-gal staining (Margaryan, Naira) 2. (no subject) (Waitts, Celeste) ---------------------------------------------------------------------- Message: 1 Date: Tue, 7 Jan 2020 18:21:31 +0000 From: "Margaryan, Naira" To: "'histonet-request (histonet at lists.utsouthwestern.edu)'" Subject: [Histonet] X-gal staining Message-ID: Content-Type: text/plain; charset="us-ascii" Hello histonetters, I have request from a scientist to perform X-gal staining on the formalin fixed paraffin-embedded kidney. I was wondering if there is any possibility to do this staining on FFPE tissue as well as if there any possibility to perform a peroxidase DAB staining for the X-gal? If so, may I ask you for a detailed protocol to perform this staining. Any input and explanation why it is impossible is appreciated. Thanks in advance, Naira ------------------------------ Message: 2 Date: Wed, 8 Jan 2020 16:46:18 +0000 From: "Waitts, Celeste" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] (no subject) Message-ID: <131bf77daa5d4ba08f9076d9afbf1f65 at exchnode1.local.centrastate.com> Content-Type: text/plain; charset="us-ascii" Question VIP6 SHORT BIOPSY RUN USING CLEARITE ANYTHING Celeste Waitts Histology Supervisor Centrastate Medical Center Freehold, NJ 07728 (732-303-5071) ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C5189a7f8df3b4f65b02708d79465e645%7C8370cf1416f34c16b83c724071654356%7C0&sdata=c06fBHXWIVgPOnNXhSw7mEFHHvThteuUFXxa5UYSlCI%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 194, Issue 4 **************************************** From paula at excaliburpathology.com Sat Jan 11 13:36:28 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Sat, 11 Jan 2020 19:36:28 +0000 (UTC) Subject: [Histonet] X-gal staining In-Reply-To: References: Message-ID: <641614704.6605266.1578771388281@mail.yahoo.com> I have done paraffin processing and sectioning of X Gal stained eyes and embryo heads. The staining is done for the blue color first, post fixed, and then processed.Xylene should be avoided, as it is what removes the X Gal target, and a xylene substitute used such as StatLab's XS3. Deparaffinization and dehydration for coverslipping should also be done with the xylene substitute. I counterstain with Nuclear Fast Red. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Saturday, January 11, 2020, 01:22:43 PM CST, Hobbs, Carl via Histonet wrote: Hi No replies so far so.....my pennyworth. Imho ...no beta Gal enzyme is inactivated by std Pwax processing. So, when you apply the X-gal substrate and chromogen solution to Pwax sections,? it will not give you a positive blue final reaction product If you want to detect beta Gal in FFPWS and visualise with DAB,? you will have to use an anti beta Gal antibody. Finding an anti beta Gal enzyme antibody, that works in FFPWS +/- AR is not so easy. Good luck Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From: histonet-request at lists.utsouthwestern.edu Sent: 08 January 2020 18:00 To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 194, Issue 4 ? Send Histonet mailing list submissions to ? ? ? ? histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ? ? ? ? https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C5189a7f8df3b4f65b02708d79465e645%7C8370cf1416f34c16b83c724071654356%7C0&sdata=c06fBHXWIVgPOnNXhSw7mEFHHvThteuUFXxa5UYSlCI%3D&reserved=0 or, via email, send a message with subject or body 'help' to ? ? ? ? histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at ? ? ? ? histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. X-gal staining (Margaryan, Naira) ? 2. (no subject) (Waitts, Celeste) ---------------------------------------------------------------------- Message: 1 Date: Tue, 7 Jan 2020 18:21:31 +0000 From: "Margaryan, Naira" To: "'histonet-request (histonet at lists.utsouthwestern.edu)'" ? ? ? ? Subject: [Histonet] X-gal staining Message-ID: ? ? ? ? ? ? ? ? Content-Type: text/plain; charset="us-ascii" Hello histonetters, I have request from a scientist to perform X-gal staining on the formalin fixed paraffin-embedded kidney. I was wondering if there is any possibility to do this staining on FFPE tissue as well as if there any possibility to perform a peroxidase DAB? staining for the X-gal? If so, may I ask you for a detailed protocol to perform this staining. Any input and explanation why it is impossible is appreciated. Thanks in advance, Naira ------------------------------ Message: 2 Date: Wed, 8 Jan 2020 16:46:18 +0000 From: "Waitts, Celeste" To: "'histonet at lists.utsouthwestern.edu'" ? ? ? ? Subject: [Histonet] (no subject) Message-ID: ? ? ? ? <131bf77daa5d4ba08f9076d9afbf1f65 at exchnode1.local.centrastate.com> Content-Type: text/plain; charset="us-ascii" Question? ? ? VIP6? ? SHORT BIOPSY RUN? ? USING CLEARITE ANYTHING Celeste Waitts Histology Supervisor Centrastate Medical Center Freehold, NJ? 07728 (732-303-5071) ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C5189a7f8df3b4f65b02708d79465e645%7C8370cf1416f34c16b83c724071654356%7C0&sdata=c06fBHXWIVgPOnNXhSw7mEFHHvThteuUFXxa5UYSlCI%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 194, Issue 4 **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From heather.etchevers at inserm.fr Sun Jan 12 14:02:44 2020 From: heather.etchevers at inserm.fr (H. Etchevers) Date: Sun, 12 Jan 2020 21:02:44 +0100 Subject: [Histonet] X-gal staining In-Reply-To: References: Message-ID: <2e38ce90-6ab9-937e-e999-cc5b13f110e7@inserm.fr> I agree with Carl about the X-gal activity being destroyed by processing for paraffin sections (I'm a researcher, not a histologist). However, in our hands the anti-b-galactosidase antibody, reference A-11132, from ThermoFisher DOES work on paraffin sections (many others don't). It has been published with subsequent incubation with a biotinylated secondary (Vector BA-1000) and standard procedures thereafter to show good signal with DAB. We do immunofluorescence. MPBio used to sell a good polyclonal for this purpose, but it has since been discontinued. Best wishes, Heather -- *Marseille Medical Genetics Centre * INSERM logo AMU logo *Heather C. Etchevers, Ph.D.* *MMG - INSERM U1251* Facult? de M?decine AMU 27 boulevard Jean Moulin 13005 Marseille - France Tel: +33(0)4 91 32 49 37 heather.etchevers at inserm.fr CREST-NET groupement de recherche CNRS GDR 2031 *CREST-NET* From amosbrooks at gmail.com Sun Jan 12 15:40:57 2020 From: amosbrooks at gmail.com (Amos Brooks) Date: Sun, 12 Jan 2020 16:40:57 -0500 Subject: [Histonet] X-gal staining In-Reply-To: References: Message-ID: > > > Hi, I'd like to concur with Carl Hobbs. With formalin fixed, paraffin embedded tissue, beta Galactosidase is definitely the way to go. It is just an antibody so you would do it like any other IHC. Amos Hi > No replies so far so.....my pennyworth. > Imho ...no > beta Gal enzyme is inactivated by std Pwax processing. > So, when you apply the X-gal substrate and chromogen solution to Pwax > sections, it will not give you a positive blue final reaction product > If you want to detect beta Gal in FFPWS and visualise with DAB, you will > have to use an anti beta Gal antibody. > Finding an anti beta Gal enzyme antibody, that works in FFPWS +/- AR is > not so easy. > > Good luck > > Carl > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge? > Kings College London > London > SE1 1UL > ? > > 020 7848 6813 > > > Subject: [Histonet] X-gal staining > > Message-ID: > > < > MN2PR05MB71011189AB120F740D180AB0A53F0 at MN2PR05MB7101.namprd05.prod.outlook.com > > > > > > Content-Type: text/plain; charset="us-ascii" > > > Hello histonetters, > > > I have request from a scientist to perform X-gal staining on the formalin > fixed paraffin-embedded kidney. > > I was wondering if there is any possibility to do this staining on FFPE > tissue as well as if there any possibility to perform a peroxidase DAB > staining for the X-gal? If so, may I ask you for a detailed protocol to > perform this staining. > > Any input and explanation why it is impossible is appreciated. > > > > Thanks in advance, > > Naira > > From 11z at comcast.net Sun Jan 12 21:36:35 2020 From: 11z at comcast.net (11z at comcast.net) Date: Sun, 12 Jan 2020 19:36:35 -0800 Subject: [Histonet] Histonet Digest, Vol 194, Issue 7 In-Reply-To: References: Message-ID: <000001d5c9c2$a8e07b60$faa17220$@comcast.net> I am looking for a small sample of normal stomach for my IHC stain control. I am willing to trade any of my control blocks if you have a block you can spare. Thanks, LeRoy Brown HT(ASCP) HTL -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Sunday, January 12, 2020 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 194, Issue 7 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From Theresa.Oeler at nsabp.org Mon Jan 13 12:09:41 2020 From: Theresa.Oeler at nsabp.org (Oeler, Theresa) Date: Mon, 13 Jan 2020 18:09:41 +0000 Subject: [Histonet] My IP address has changed please up date so I may remain on the list. Message-ID: Update IP address! Thanks! Theresa Oeler NSABP Foundation, Inc. Federal North Building 1307 Federal Street - Suite 303 Pittsburgh, PA 15212 O # 412-697-6609 F # 412-697-6613 Toeler at nsabp.org From john.bigbee at vcuhealth.org Mon Jan 13 15:47:10 2020 From: john.bigbee at vcuhealth.org (John Bigbee) Date: Mon, 13 Jan 2020 21:47:10 +0000 Subject: [Histonet] =?windows-1252?q?Announcing_Digital_Histology_=96_A_fr?= =?windows-1252?q?ee-use=2C_open-access_instructional_package?= Message-ID: Dear colleagues, We are very pleased to announce our new free-use, open-access instructional package entitled Digital Histology. The package is compatible with both PC and Mac computers and is mobile friendly. Digital Histology can be used by a diverse group of learners and is available free of charge at https://digitalhistology.org. Digital Histology is organized as chapters that parallel those of most histology textbooks. Each of the over 1600 pages contains an original, high quality image accompanied by descriptive text and selectable labels. In addition, interactive quizzes with formative feedback accompany each chapter of Digital Histology. A review textbook with hyperlinks to images in the main package is also included. A brief introductory video is available here: Introduction to Digital Histology We invite you to take a look at Digital Histology and hope you find it to be a useful teaching and learning resource. Our best wishes, John W. Bigbee, Ph.D. Professor, Department of Anatomy and Neurobiology Virginia Commonwealth University (john.bigbee at vcuhealth.org) Alice S. Pakurar, Ph.D. Associate Professor (Retired), Department of Anatomy and Neurobiology Virginia Commonwealth University From thisisann at aol.com Tue Jan 14 09:07:04 2020 From: thisisann at aol.com (Ann Specian) Date: Tue, 14 Jan 2020 15:07:04 +0000 (UTC) Subject: [Histonet] Change in Histotech training/educational requirements References: <706933368.7476226.1579014424850.ref@mail.yahoo.com> Message-ID: <706933368.7476226.1579014424850@mail.yahoo.com> Can someone tell me if there has been a change in the training and education requirements in?the New Jersey/New York area for Histotech?s. Were there any regulatory changes? Sent from AOL Mobile Mail Get the new AOL app: mail.mobile.aol.com From relia1 at earthlink.net Wed Jan 15 10:51:04 2020 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 15 Jan 2020 11:51:04 -0500 Subject: [Histonet] RELIA HOT Job Alert. Opportunities nationwide FL, NC, SC, TN, TX, KS, CA, IL and MORE! Message-ID: <000001d5cbc3$f9ffa680$edfef380$@earthlink.net> Hello Histonetterss, I hope you are having a great week. Did you get a chance to check out my Brand new newsletter? If not here is a link: https://reliasolutionspambarker.wordpress.com/2020/01/10/introducing-relia-s olutions-histology-careers-newsletter/ I wanted to send a quick note to tell you about the positions that I am working on and am most excited about.? All of these positions are full time and permanent!! My clients offer excellent compensation, benefits and in most cases either relocation or a sign-on bonus. Histonetters, Here are the openings I am working on from these clients!! KS ? Topeka Histotech IL ? Chicagoland IHC Field Applications Specialist FL ? Orlando Histotechnology Program Director FL ? Ft Myers Histotechnologist 10K sign on bonus!! NC ? Greensboro Histotech TN ? Kingsport Histotech TN- Nashville Night Shift Supervisor SC ? Ridgeland Histotech TX ? Austin Histotech CA ? Modesto Histotech If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. ? If I place someone you refer You will earn a referral fee. ? If you refer someone to subscribe to my bulletins and I place them you will earn a referral fee. If you are interested in any of these opportunities CALL /TEXT MY CELL ASAP!!! At 407-353-5070. I can also be reached toll free at the office at 866-607-3542 or at relia1 at earthlink.net Happy New Year!! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Prabhakar.Ashwin at hcahealthcare.com Wed Jan 15 12:06:36 2020 From: Prabhakar.Ashwin at hcahealthcare.com (Ashwin Ashwin) Date: Wed, 15 Jan 2020 18:06:36 +0000 Subject: [Histonet] Job Opportunity Message-ID: Histology Technologist II Full-time: https://careers.hcahealthcare.com/jobs/4928406-histology-technologist-ii Prabhakar Ashwin Regional Director of Histology Ph: (954) 777-0018 Ext-260 IRL Ft Lauderdale 5361 NW 33rd Ave, Fort Lauderdale, FL 33309 HCA | Hospital Corporation of America -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Wednesday, January 15, 2020 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: {EXTERNAL} Histonet Digest, Vol 194, Issue 10 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=9fZnZOgPWmHmvevlab4V4DSjtBMjorSlbQYfK_MauDg&r=mAQEBd6CZoGLdw8_4q7KmqwvXMFxKOklfk484LTCdwG_1K1rwlVShXuPZbYK0XmP&m=w7pz_iCBTbPLLvTe6reps37E32X508bRc_VOsybMlo0&s=mk8n8RD0Htb54BVpLgqVJP6cGZAiGAxJYeHTseQk98Y&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From 11z at comcast.net Wed Jan 15 12:30:15 2020 From: 11z at comcast.net (11z at comcast.net) Date: Wed, 15 Jan 2020 10:30:15 -0800 Subject: [Histonet] Histonet Digest, Vol 194, Issue 10 In-Reply-To: References: Message-ID: <000101d5cbd1$d5814580$8083d080$@comcast.net> How do I sign up one of my employees to be given NSH membership. What is the cost? Thanks, LeRoy Brown HT(ASCP) HTL -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Wednesday, January 15, 2020 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 194, Issue 10 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From wangweixi at sjhmc.org Wed Jan 15 12:20:19 2020 From: wangweixi at sjhmc.org (Wang, Weixi) Date: Wed, 15 Jan 2020 18:20:19 +0000 Subject: [Histonet] H&E trouble shooting Message-ID: Hi, I have some issues on the staining quality of biopsies. Please see the image link below, the overall staining looks good but the edge looks weird, no nuclear staining picked up. Under-fixed? Over-dehydrated in the processor? Or dried during grossing? You expertise is greatly appreciated! https://imgur.com/gallery/kpLqRT9 Thank you, Weixi *** Important Notice About St. Josephs emails *** St. Josephs Regional Medical Center is using Zix to encrypt any e-mails containing Protected Health Information (PHI). If you receive an encrypted email from a person at St. Josephs the body of the message will indicate that you have a "New Zixcorp secure email from St. Josephs Regional Medical Center." You will need to click on the link in that email to retrieve the message. For more information please visit http://www.uapguide.com/st-josephs-hospital-and-regional-medical-center/introduction. For help in retrieving a secure email that you?ve received from St Josephs please go to http://www.uapguide.com/st-josephs-hospital-and-regional-medical-center/receiving-encrypted-email. CONFIDENTIAL COMMUNICATION THIS TRANSMISSION IS INTENDED ONLY FOR THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND CONTAINS INFORMATION THAT IS CONFIDENTIAL. IF YOU HAVE RECEIVED THIS COMMUNICATION IN ERROR, PLEASE DELETE THE EMAIL AND CONTACT THE SENDER IMMEDIATELY. THIS INFORMATION MAY HAVE BEEN DISCLOSED TO YOU FROM CONFIDENTIAL RECORDS AND MAY BE PROTECTED BY FEDERAL AND STATE LAW. THIS INFORMATION MAY INCLUDE CONFIDENTIAL MENTAL HEALTH, SUBSTANCE ABUSE, ALCOHOL ABUSE AND/OR HIV-RELATED INFORMATION. FEDERAL AND STATE LAW PROHIBITS YOU FROM MAKING ANY FURTHER DISCLOSURE OF THIS INFORMATION WITHOUT THE SPECIFIC WRITTEN CONSENT OF THE PERSON TO WHOM IT PERTAINS, OR AS OTHERWISE PERMITTED BY LAW. ANY UNAUTHORIZED FURTHER DISCLOSURE IN VIOLATION OF THE LAW MAY RESULT IN A FINE OR JAIL SENTENCE OR BOTH. A GENERAL AUTHORIZATION FOR THE RELEASE OF THIS INFORMATION MAY NOT BE SUFFICIENT AUTHORIZATION FOR FURTHER DISCLOSURE. From liz at premierlab.com Wed Jan 15 12:49:27 2020 From: liz at premierlab.com (Liz Chlipala) Date: Wed, 15 Jan 2020 18:49:27 +0000 Subject: [Histonet] H&E trouble shooting In-Reply-To: References: Message-ID: That an image of incomplete dehydration the samples have not been processed properly and adequately dehydrated. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Wang, Weixi via Histonet Sent: Wednesday, January 15, 2020 11:20 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] H&E trouble shooting Hi, I have some issues on the staining quality of biopsies. Please see the image link below, the overall staining looks good but the edge looks weird, no nuclear staining picked up. Under-fixed? Over-dehydrated in the processor? Or dried during grossing? You expertise is greatly appreciated! https://imgur.com/gallery/kpLqRT9 Thank you, Weixi *** Important Notice About St. Josephs emails *** St. Josephs Regional Medical Center is using Zix to encrypt any e-mails containing Protected Health Information (PHI). If you receive an encrypted email from a person at St. Josephs the body of the message will indicate that you have a "New Zixcorp secure email from St. Josephs Regional Medical Center." You will need to click on the link in that email to retrieve the message. For more information please visit http://www.uapguide.com/st-josephs-hospital-and-regional-medical-center/introduction. For help in retrieving a secure email that you've received from St Josephs please go to http://www.uapguide.com/st-josephs-hospital-and-regional-medical-center/receiving-encrypted-email. CONFIDENTIAL COMMUNICATION THIS TRANSMISSION IS INTENDED ONLY FOR THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND CONTAINS INFORMATION THAT IS CONFIDENTIAL. IF YOU HAVE RECEIVED THIS COMMUNICATION IN ERROR, PLEASE DELETE THE EMAIL AND CONTACT THE SENDER IMMEDIATELY. THIS INFORMATION MAY HAVE BEEN DISCLOSED TO YOU FROM CONFIDENTIAL RECORDS AND MAY BE PROTECTED BY FEDERAL AND STATE LAW. THIS INFORMATION MAY INCLUDE CONFIDENTIAL MENTAL HEALTH, SUBSTANCE ABUSE, ALCOHOL ABUSE AND/OR HIV-RELATED INFORMATION. FEDERAL AND STATE LAW PROHIBITS YOU FROM MAKING ANY FURTHER DISCLOSURE OF THIS INFORMATION WITHOUT THE SPECIFIC WRITTEN CONSENT OF THE PERSON TO WHOM IT PERTAINS, OR AS OTHERWISE PERMITTED BY LAW. ANY UNAUTHORIZED FURTHER DISCLOSURE IN VIOLATION OF THE LAW MAY RESULT IN A FINE OR JAIL SENTENCE OR BOTH. A GENERAL AUTHORIZATION FOR THE RELEASE OF THIS INFORMATION MAY NOT BE SUFFICIENT AUTHORIZATION FOR FURTHER DISCLOSURE. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From katherine at ka-recruiting.com Thu Jan 16 09:23:20 2020 From: katherine at ka-recruiting.com (Katherine Marano) Date: Thu, 16 Jan 2020 10:23:20 -0500 Subject: [Histonet] Histotech openings Message-ID: Hi histonetters~ I'm working with a top laboratory in a beautiful area of North Carolina that is looking to hire histotechs. They currently have 2 positions open. The hours are varied from 10 pm ? 12 noon. They?ll either be 3rd shift or a split 3rd/1st shift. Permanent positions with great benefits. Let me know if you are interested in hearing more! Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From criley at dpspa.com Thu Jan 16 10:55:21 2020 From: criley at dpspa.com (Charles Riley) Date: Thu, 16 Jan 2020 11:55:21 -0500 Subject: [Histonet] Cell block preparations Message-ID: What is the best way to remove excess blood from FNA sample collections before spinning them down into cell blocks? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From jmacdonald at mtsac.edu Thu Jan 16 11:53:03 2020 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Thu, 16 Jan 2020 17:53:03 +0000 Subject: [Histonet] Cell block preparations In-Reply-To: References: Message-ID: Acetic acid would work. Get Outlook for iOS ________________________________ From: Charles Riley via Histonet Sent: Thursday, January 16, 2020 8:55:21 AM To: Histo List Subject: [Histonet] Cell block preparations EXTERNAL SENDER- Exercise caution with requests, links, and attachments. What is the best way to remove excess blood from FNA sample collections before spinning them down into cell blocks? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From 11z at comcast.net Thu Jan 16 12:10:03 2020 From: 11z at comcast.net (LeRoy Brown) Date: Thu, 16 Jan 2020 10:10:03 -0800 Subject: [Histonet] needing IHC contol tissue Message-ID: <007e01d5cc98$2df369c0$89da3d40$@comcast.net> Would anyone happen to have a small pc of normal stomach they are willing to part with for my IHC control for GAS6 stain? Please let me know at 11z at comcast.net Thanks LeRoy Brown HT(ASCP) HTL From albert.dotson at duke.edu Thu Jan 16 12:09:29 2020 From: albert.dotson at duke.edu (Bert Dotson) Date: Thu, 16 Jan 2020 18:09:29 +0000 Subject: [Histonet] H&E trouble shooting In-Reply-To: References: Message-ID: Excessive heat during slide drying can look like this but I think Liz has the most likely culprit. More specifically I would say there has been water carryover into your first clearing station during processing. Reagents not changed frequently enough or a low-grade mistakenly placed where 100% should be. Regards, Bert -----Original Message----- From: Liz Chlipala Sent: Wednesday, January 15, 2020 1:49 PM To: Wang, Weixi Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] H&E trouble shooting That an image of incomplete dehydration the samples have not been processed properly and adequately dehydrated. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Wang, Weixi via Histonet Sent: Wednesday, January 15, 2020 11:20 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] H&E trouble shooting Hi, I have some issues on the staining quality of biopsies. Please see the image link below, the overall staining looks good but the edge looks weird, no nuclear staining picked up. Under-fixed? Over-dehydrated in the processor? Or dried during grossing? You expertise is greatly appreciated! https://urldefense.proofpoint.com/v2/url?u=https-3A__imgur.com_gallery_kpLqRT9&d=DwIFAg&c=imBPVzF25OnBgGmVOlcsiEgHoG1i6YHLR0Sj_gZ4adc&r=IkXb8WHm-SU8QcloSa5zh99VMreL429Dgfd2s0o3mrA&m=9Zt4pLGTo6dCvnjHleUlTkBYb8LQQc2G52vrJRkdg9M&s=8qzwuTTHy5TnitTfvT39e_57wxXJFJ2nygQXLWjNxVo&e= Thank you, Weixi *** Important Notice About St. Josephs emails *** St. Josephs Regional Medical Center is using Zix to encrypt any e-mails containing Protected Health Information (PHI). If you receive an encrypted email from a person at St. Josephs the body of the message will indicate that you have a "New Zixcorp secure email from St. Josephs Regional Medical Center." You will need to click on the link in that email to retrieve the message. For more information please visit https://urldefense.proofpoint.com/v2/url?u=http-3A__www.uapguide.com_st-2Djosephs-2Dhospital-2Dand-2Dregional-2Dmedical-2Dcenter_introduction&d=DwIFAg&c=imBPVzF25OnBgGmVOlcsiEgHoG1i6YHLR0Sj_gZ4adc&r=IkXb8WHm-SU8QcloSa5zh99VMreL429Dgfd2s0o3mrA&m=9Zt4pLGTo6dCvnjHleUlTkBYb8LQQc2G52vrJRkdg9M&s=UzAs7b3GXocbvkjR-MIIe6DsHF6sRik5gbgS4bHc8X0&e= . For help in retrieving a secure email that you've received from St Josephs please go to https://urldefense.proofpoint.com/v2/url?u=http-3A__www.uapguide.com_st-2Djosephs-2Dhospital-2Dand-2Dregional-2Dmedical-2Dcenter_receiving-2Dencrypted-2Demail&d=DwIFAg&c=imBPVzF25OnBgGmVOlcsiEgHoG1i6YHLR0Sj_gZ4adc&r=IkXb8WHm-SU8QcloSa5zh99VMreL429Dgfd2s0o3mrA&m=9Zt4pLGTo6dCvnjHleUlTkBYb8LQQc2G52vrJRkdg9M&s=Gy141DOHMNtR-oJ6lFs8ERhL4hg4SJq3e8O2Cp-rV0M&e= . CONFIDENTIAL COMMUNICATION THIS TRANSMISSION IS INTENDED ONLY FOR THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND CONTAINS INFORMATION THAT IS CONFIDENTIAL. IF YOU HAVE RECEIVED THIS COMMUNICATION IN ERROR, PLEASE DELETE THE EMAIL AND CONTACT THE SENDER IMMEDIATELY. THIS INFORMATION MAY HAVE BEEN DISCLOSED TO YOU FROM CONFIDENTIAL RECORDS AND MAY BE PROTECTED BY FEDERAL AND STATE LAW. THIS INFORMATION MAY INCLUDE CONFIDENTIAL MENTAL HEALTH, SUBSTANCE ABUSE, ALCOHOL ABUSE AND/OR HIV-RELATED INFORMATION. FEDERAL AND STATE LAW PROHIBITS YOU FROM MAKING ANY FURTHER DISCLOSURE OF THIS INFORMATION WITHOUT THE SPECIFIC WRITTEN CONSENT OF THE PERSON TO WHOM IT PERTAINS, OR AS OTHERWISE PERMITTED BY LAW. ANY UNAUTHORIZED FURTHER DISCLOSURE IN VIOLATION OF THE LAW MAY RESULT IN A FINE OR JAIL SENTENCE OR BOTH. A GENERAL AUTHORIZATION FOR THE RELEASE OF THIS INFORMATION MAY NOT BE SUFFICIENT AUTHORIZATION FOR FURTHER DISCLOSURE. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIFAg&c=imBPVzF25OnBgGmVOlcsiEgHoG1i6YHLR0Sj_gZ4adc&r=IkXb8WHm-SU8QcloSa5zh99VMreL429Dgfd2s0o3mrA&m=9Zt4pLGTo6dCvnjHleUlTkBYb8LQQc2G52vrJRkdg9M&s=bfcPZowI5Jn6SobgT6ri3RslREo2h4yS2rI_wEOfAto&e= ________________________________ From Karen.Heckford at DignityHealth.org Thu Jan 16 13:25:39 2020 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Thu, 16 Jan 2020 19:25:39 +0000 Subject: [Histonet] RVU for Histology Message-ID: <7f90eb19cba54e949acbe0a6f793f434@PHX-EXCH-013.chw.edu> Good Morning, I think we are getting the short end of the stick when it is coming to productivity. Can anyone give me insight on how they are calculating their RVU's for productivity in the Histology Lab? I am busy as all get out and administration is saying our productivity is low. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you From naira.margaryan at hsc.wvu.edu Thu Jan 16 14:54:28 2020 From: naira.margaryan at hsc.wvu.edu (Margaryan, Naira) Date: Thu, 16 Jan 2020 20:54:28 +0000 Subject: [Histonet] Coverslipper In-Reply-To: References: Message-ID: Happy Thursday, We would like to sell our slightly used but in excellent condition Dako Coverslipper which was purchased in 2010 (purchase price was $ 25K). The instrument can handle up to 600 slides per hour making it one of the fastest on the market. In addition to the flexibility of the Coverslipper it is easy and straightforward to operate and cleaning and maintenance is simple to do. It is small enough to fit into fume cabinets, easy to move around and accepts a variety of commercial mounting media. Picture by request. Will take any offer, Naira From tony.henwood at health.nsw.gov.au Thu Jan 16 16:24:14 2020 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 16 Jan 2020 22:24:14 +0000 Subject: [Histonet] Cell block preparations In-Reply-To: References: Message-ID: Hi Jennifer, I have had excellent success with lysing the red blood cells (using Isotonic Ammonium Chloride) prior to cell block preparation with thromboplastin-plasma. The lysing solution contains EDTA so you will need to add a few drops of 1% calcium chloride. Method as follows: Lysis solution Ammonium Chloride 4.5g Potassium carbonate 0.5g EDTA 0.0186g Distilled water 500mls Method: 1. Centrifuge bloody fluid. 2. Remove supernatant and add equal volume of lysis solution. 3. Resuspend and incubate for 5 minutes at 4oC. 4. Centrifuge, if blood still remains, then repeat from step 2. 5. Rinse in Hanks or RPMI, centrifuge. 6. Mix pellet in a few drops of plasma. 7. Add thromboplastin and a few drops of 1% Calcium Chloride, mix gently and allow clot to form. 8. Add 10% buffered formalin and fix and process as usual. Reference: Kuenen-Boumiester etal (1996) Acta Cytolog 40:475-479 If you donot use the plasma clot method for cell block preparation, then use your preferred method after step 5. The lysis solution can also be purchased commercially from several companies (eg Biolegend). It is commonly used for sample preparation for flow cytometry. Check the SDS to make sure it does not contain formaldehyde. -----Original Message----- From: Mac Donald, Jennifer via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 17 January 2020 4:53 AM To: Charles Riley ; Histo List Subject: Re: [Histonet] Cell block preparations Acetic acid would work. Get Outlook for iOS ________________________________ From: Charles Riley via Histonet Sent: Thursday, January 16, 2020 8:55:21 AM To: Histo List Subject: [Histonet] Cell block preparations EXTERNAL SENDER- Exercise caution with requests, links, and attachments. What is the best way to remove excess blood from FNA sample collections before spinning them down into cell blocks? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From tonyreilly55 at gmail.com Fri Jan 17 03:37:35 2020 From: tonyreilly55 at gmail.com (Tony Reilly) Date: Fri, 17 Jan 2020 19:37:35 +1000 Subject: [Histonet] Cell block preparations In-Reply-To: References: Message-ID: Hi Charles A method my EM scientist used many years ago was quite simplistic and he thought it worked well. You simply centrifuge the specimen, add a set volume of deionised water, mix to lose the red cells, then add an equal volume of double strength normal saline to create a suspension in normal saline. The centrifuge where the heavier uncleared cells go to the bottom of the tube. I have to say the I have never tried this personally but if the morphology was retained for EM it must be fine for LM. Having said that I have great respect for Tony Henwood and his method looks much more scientific. Regards Tony Sent from my iPhone > On 17 Jan 2020, at 8:24 am, Tony Henwood (SCHN) via Histonet wrote: > > Hi Jennifer, > > I have had excellent success with lysing the red blood cells (using Isotonic Ammonium Chloride) prior to cell block preparation with thromboplastin-plasma. > The lysing solution contains EDTA so you will need to add a few drops of 1% calcium chloride. Method as follows: > > Lysis solution > Ammonium Chloride 4.5g > Potassium carbonate 0.5g > EDTA 0.0186g > Distilled water 500mls > > Method: > 1. Centrifuge bloody fluid. > 2. Remove supernatant and add equal volume of lysis solution. > 3. Resuspend and incubate for 5 minutes at 4oC. > 4. Centrifuge, if blood still remains, then repeat from step 2. > 5. Rinse in Hanks or RPMI, centrifuge. > 6. Mix pellet in a few drops of plasma. > 7. Add thromboplastin and a few drops of 1% Calcium Chloride, mix gently and allow clot to form. > 8. Add 10% buffered formalin and fix and process as usual. > > Reference: > Kuenen-Boumiester etal (1996) Acta Cytolog 40:475-479 > > If you donot use the plasma clot method for cell block preparation, then use your preferred method after step 5. > The lysis solution can also be purchased commercially from several companies (eg Biolegend). It is commonly used for sample preparation for flow cytometry. Check the SDS to make sure it does not contain formaldehyde. > > > -----Original Message----- > From: Mac Donald, Jennifer via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Friday, 17 January 2020 4:53 AM > To: Charles Riley ; Histo List > Subject: Re: [Histonet] Cell block preparations > > Acetic acid would work. > > Get Outlook for iOS ________________________________ > From: Charles Riley via Histonet > Sent: Thursday, January 16, 2020 8:55:21 AM > To: Histo List > Subject: [Histonet] Cell block preparations > > EXTERNAL SENDER- Exercise caution with requests, links, and attachments. > > What is the best way to remove excess blood from FNA sample collections before spinning them down into cell blocks? > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tonyreilly55 at gmail.com Fri Jan 17 07:08:06 2020 From: tonyreilly55 at gmail.com (Tony Reilly) Date: Fri, 17 Jan 2020 23:08:06 +1000 Subject: [Histonet] Cell block preparations In-Reply-To: References: Message-ID: <032A76F1-177D-4990-A58D-569B0766B7C0@gmail.com> Some predictive text issues like lyse and nucleated. Sent from my iPhone > On 17 Jan 2020, at 8:24 am, Tony Henwood (SCHN) via Histonet wrote: > > Hi Jennifer, > > I have had excellent success with lysing the red blood cells (using Isotonic Ammonium Chloride) prior to cell block preparation with thromboplastin-plasma. > The lysing solution contains EDTA so you will need to add a few drops of 1% calcium chloride. Method as follows: > > Lysis solution > Ammonium Chloride 4.5g > Potassium carbonate 0.5g > EDTA 0.0186g > Distilled water 500mls > > Method: > 1. Centrifuge bloody fluid. > 2. Remove supernatant and add equal volume of lysis solution. > 3. Resuspend and incubate for 5 minutes at 4oC. > 4. Centrifuge, if blood still remains, then repeat from step 2. > 5. Rinse in Hanks or RPMI, centrifuge. > 6. Mix pellet in a few drops of plasma. > 7. Add thromboplastin and a few drops of 1% Calcium Chloride, mix gently and allow clot to form. > 8. Add 10% buffered formalin and fix and process as usual. > > Reference: > Kuenen-Boumiester etal (1996) Acta Cytolog 40:475-479 > > If you donot use the plasma clot method for cell block preparation, then use your preferred method after step 5. > The lysis solution can also be purchased commercially from several companies (eg Biolegend). It is commonly used for sample preparation for flow cytometry. Check the SDS to make sure it does not contain formaldehyde. > > > -----Original Message----- > From: Mac Donald, Jennifer via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Friday, 17 January 2020 4:53 AM > To: Charles Riley ; Histo List > Subject: Re: [Histonet] Cell block preparations > > Acetic acid would work. > > Get Outlook for iOS ________________________________ > From: Charles Riley via Histonet > Sent: Thursday, January 16, 2020 8:55:21 AM > To: Histo List > Subject: [Histonet] Cell block preparations > > EXTERNAL SENDER- Exercise caution with requests, links, and attachments. > > What is the best way to remove excess blood from FNA sample collections before spinning them down into cell blocks? > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jess.hall at wvumedicine.org Fri Jan 17 08:04:11 2020 From: jess.hall at wvumedicine.org (Hall, Jessica) Date: Fri, 17 Jan 2020 14:04:11 +0000 Subject: [Histonet] WVU Medicine Message-ID: <437FE806DAB7B441A526B805E086396E420E3F6D@NT-EX2.wvuhs.com> Good Morning, We have recently opened a new Histotechnologist position with WVU Medicine! This position is eligible for a generous sign-on bonus, full comprehensive benefit package, and a competitive salary range. I invite you to contact me with any questions about this exciting opportunity to join the region's largest health system! To submit your application for consideration, click here: https://re12.ultipro.com/WES1019WVUH/jobboard/NewCandidateExt.aspx?__JobID=48450 WVU Medicine - Work Here, Live Here, Thrive Here - Click Here to watch video about our Health System / Jessica Hall, CIR, SHRM-CP, AASPR, STA Supervisor, Talent Acquisition Human Resources WVU Medicine Office: 304-598-6689 Cell: 304-709-4783 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From sbaldwin at mhhcc.org Fri Jan 17 09:44:44 2020 From: sbaldwin at mhhcc.org (Baldwin, Kathy) Date: Fri, 17 Jan 2020 15:44:44 +0000 Subject: [Histonet] VERBAGE FOR CUTTING MULTIPLE SPECIMENS ON MICROTOME Message-ID: <7ba14966a18d4484a38493080267b300@exch1.mhhcc.org> Standard protocol for replacing microtome blades anybody have this or can help?? Thanks S Kathy Baldwin ASCP, SCT Memorial Hospital and Health Care Center Pathology and Cytology Manager Ph 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From djemge11 at gmail.com Fri Jan 17 10:16:41 2020 From: djemge11 at gmail.com (Donna Emge) Date: Fri, 17 Jan 2020 10:16:41 -0600 Subject: [Histonet] Skin Sample with Tattoo - need to remove for diagnosis Message-ID: Hello fellow histonetters, We received a skin sample that has ink from a tattoo - the sample if from tattooed skin. One of our pathologists would like us to see if we can get rid of the tattoo ink from the sections before H&E staining. Does anyone out there know how to do this? Thank you, Donna Emge Anatomic Pathology Manager Mercy Hospital and Medical Center, Chicago -- From john.garratt at ciqc.ca Fri Jan 17 11:11:38 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Fri, 17 Jan 2020 17:11:38 +0000 Subject: [Histonet] Skin Sample with Tattoo - need to remove for diagnosis In-Reply-To: References: Message-ID: It is a pigment (ie carbon) and not like a soluble ink, so good luck with that. You could consult your histotech text book on exogenous pigment removal and give the recipe a go. I will certainly be interested in other histonet replies. John www.cpqa.ca ??????? Original Message ??????? On Friday, January 17, 2020 8:16 AM, Donna Emge via Histonet wrote: > Hello fellow histonetters, > > We received a skin sample that has ink from a tattoo - the sample if from > tattooed skin. One of our pathologists would like us to see if we can get > rid of the tattoo ink from the sections before H&E staining. Does anyone > out there know how to do this? > > Thank you, > Donna Emge > Anatomic Pathology Manager > Mercy Hospital and Medical Center, Chicago > > ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at dpspa.com Fri Jan 17 11:36:29 2020 From: criley at dpspa.com (Charles Riley) Date: Fri, 17 Jan 2020 12:36:29 -0500 Subject: [Histonet] Skin Sample with Tattoo - need to remove for diagnosis In-Reply-To: References: Message-ID: The only way I know of is through lasers On Fri, Jan 17, 2020 at 12:25 PM John Garratt via Histonet < histonet at lists.utsouthwestern.edu> wrote: > It is a pigment (ie carbon) and not like a soluble ink, so good luck with > that. > You could consult your histotech text book on exogenous pigment removal > and give the recipe a go. I will certainly be interested in other histonet > replies. > > John > > > > www.cpqa.ca > > ??????? Original Message ??????? > On Friday, January 17, 2020 8:16 AM, Donna Emge via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hello fellow histonetters, > > > > We received a skin sample that has ink from a tattoo - the sample if from > > tattooed skin. One of our pathologists would like us to see if we can get > > rid of the tattoo ink from the sections before H&E staining. Does anyone > > out there know how to do this? > > > > Thank you, > > Donna Emge > > Anatomic Pathology Manager > > Mercy Hospital and Medical Center, Chicago > > > > > ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- > > > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From relia1 at earthlink.net Fri Jan 17 12:14:46 2020 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 17 Jan 2020 13:14:46 -0500 Subject: [Histonet] FW: New Post: Strategies for Coping With the Shortage of Histotechs Message-ID: <00e501d5cd62$00524b60$00f6e220$@earthlink.net> Hi Histonetters! Here is my latest blog post on the NSH - Fixation on Histology site: "Strategies for Coping with the Shortage of Histotechs. TGIF. Have a wonderful weekend! Thanks-Pam From: Fixation on Histology [mailto:histo at nsh.org] Sent: Friday, January 17, 2020 1:07 PM To: relia1 at earthlink.net Subject: [SPAM] New Post: Strategies for Coping With the Shortage of Histotechs Please click here to access the web/mobile version fixationonhistology_60470.PNG Check out Fixation on Histology's Newest Post: Strategies for Coping With the Shortage of Histotechs By: Pam Barker, Relia Solutions needjob_960971.PNG Has there ever been a time when there wasn't a shortage of histotechs? It just seems to get more critical every year. I have to be honest I would LOVE to place techs with all of you but they aren't always out there. I wanted to write this article to encourage you and give you strategies to cope with the shortage because I think we can all agree that this shortage is not going away. Continue Reading _____ Interested in Contributing? NSH is accepting submissions for Fixation on Histology! Share your stories, advice, or research with others in the histology profession! Submit Your Post National Society for Histotechnology National Society for Histotechnology 3545 Ellicott Mills Dr. Ellicott City, MD 21043 Phone: 443:535-4060 Fax: 443-535-4055 Email: histo at nsh.org Follow Us: facebook twitter linkedin linkedin Subscribe to our Blog: Fixation on Histology Unsubscribe from these messages. 3545 Ellicott Mills Drive, Ellicott City, MD 21043 From blayjorge at gmail.com Fri Jan 17 12:53:02 2020 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Fri, 17 Jan 2020 13:53:02 -0500 Subject: [Histonet] Prepared slides of human skin with tattoos in xs Message-ID: Hi: I teach college human anatomy and physiology and would like to have a slide or two of human skin with tattoos in cross section. Do you know where may I get a hold of those? All I use these days is an illustration from the web. Please, email me directly if you have constructive suggestion. blaayjorge at gmail.com Gratefully, Jorge Jorge A. Santiago-Blay, PhD *https://blaypublishers.com * 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From Dawn.Olszewski at SGMC.ORG Fri Jan 17 12:53:18 2020 From: Dawn.Olszewski at SGMC.ORG (Olszewski, Dawn) Date: Fri, 17 Jan 2020 18:53:18 +0000 Subject: [Histonet] 3rd party block and slide storage fees References: <12a3486f-aba0-466f-9fef-6a4ca11e0a30.3b5c5640-2195-4f35-b5ef-5e1ebee68512.8e8fe3a3-45a0-4df7-91cd-446a29c1dbc5@emailsignatures365.codetwo.com> Message-ID: Hi everyone, We are a smaller hospital that stores our blocks, slides and files off site with a 3rd party storage facility. Does anyone have any information on fees for storage of blocks, slides, records and how much they charge to pick-up and deliver when needed and the names of any companies you prefer to use? Thank you all as always for your help. Dawn Olszewski, HTL(ASCP)QIHC Pathology Manager South Georgia Medical Center P: (229) 259-4830 E: dawn.olszewski at sgmc.org Dawn Olszewski Pathology Manager - Histotechnologist Laboratory [cid:MasterLogo_190x61_362365a5-c941-4f16-9f99-9484fd6a6078.png] South Georgia Medical Center 2501 N. Patterson St. Valdosta, GA 31602 229-259-4830 (O) | 229-560-6191 (M) Dawn.Olszewski at SGMC.ORG | sgmc.org [cid:facebook_fb_32x32_0a6d9065-09e0-48f7-88c4-b2d459a5e547.png] [cid:twitter_32x32_5a3a5eeb-77c7-4a3c-aa09-b69b41f32bd2.png] [cid:linkedin_ln_32x32_bf1e5dc9-b886-480a-8c8b-ef8a1ae87168.png] [cid:youtube_play_32x32_572cd3ed-390b-48e0-8845-ba1f81953de3.png] From SteveM at mcclainlab.com Fri Jan 17 12:56:51 2020 From: SteveM at mcclainlab.com (Steve McClain) Date: Fri, 17 Jan 2020 18:56:51 +0000 Subject: [Histonet] Histonet Digest, Vol 194, Issue 12btattoo removal In-Reply-To: References: Message-ID: <8C35DA0C-E8A4-443A-BB6E-D5EFE5549161@mcclainlab.com> I don not know any method for tattoo pigment removal. Aside from carbon black, most of the red, yellow, blue and green pigments are metal salts and polarize brightly. The inflammatory response may be vigorous, especially w repeat or Re-do of a tattoo. In some cases a pseudo-carcinoma or KA may result. I am puzzled to learn why the request! Can anyone in the group think of a reason why or purpose for the pathologist needs to request removal? Perhaps Staining of infectious organism? Steve Steve A. McClain, MD 631-361-4000 Cell 631-926-3655 Hello fellow histonetters, We received a skin sample that has ink from a tattoo - the sample if from tattooed skin. One of our pathologists would like us to see if we can get rid of the tattoo ink from the sections before H&E staining. Does anyone out there know how to do this? Thank you, Donna Emge Anatomic Pathology Manager Mercy Hospital and Medical Center, Chicago From acanabal at ciencias.unam.mx Fri Jan 17 15:29:08 2020 From: acanabal at ciencias.unam.mx (=?UTF-8?Q?Alonso_Mart=C3=ADnez_Canabal?=) Date: Fri, 17 Jan 2020 15:29:08 -0600 Subject: [Histonet] Question about gelatin embedding Message-ID: Hello, I am here again. I am wondering if someone has good experience embedding in gelatin-albumin for cryostat or vibratome sectioning. Specifically we use brain tissue and is common in free floating techniques non-attached parts of the same section float around and later that generates all sorts of problems. Thank you very much. -- Dr. Alonso Mart?nez Canabal PhD Profesor Asociado "C" Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833 From histology400 at gmail.com Sat Jan 18 06:21:07 2020 From: histology400 at gmail.com (Valerie Laughlin) Date: Sat, 18 Jan 2020 04:21:07 -0800 Subject: [Histonet] Pregnant in histo lab. Am I safe? Message-ID: Hello everyone. I am currently in the last weeks of my first trimester of my pregnancy. I have asked this question to my Ob-Gyn, family and general pregnancy forums but I wanted to ask people who understand the field of Histotechnology better. I have been very concerned about the side effects of the chemicals that might have on my baby. The lab works with the typical stuff (formaldehyde, xylene, alcohol of different percentages, glacial acetic acid, stains etc) They make the fixative from scratch. I had to inform my supervisor and manager. I didn?t get the most positive reaction from them but I don?t care as this is my personal business and I have rights like everybody else. I gave them a letter from my doctor informing my pregnancy and that I should be kept away from the chemicals for my own safety. They acknowledged the letter but still decided to buy a respirator mask for me which is fine. It?s good to have protective equipment no matter the circumstance. I told them that I can do the same tasks I do every day such as grossing but with a mask, embedding, cutting and filing but that I don?t feel comfortable changing the chemicals of the tissue processor and slide stainer, and mixing chemicals. Also that I can?t dump the chemicals in the biohazard room as there is not enough ventilation. Literally an hour after I informed this a nurse who was working in a rojom close to the biohazard room had a negative reaction and had to be sent to the ER where she was there for days. She blamed the chemicals from the biohazard room. Other nurses who work close to that room had reported negative side effects as well. This situation made me more uncomfortable specially when my coworkers think the nurses are over reacting and it has to be some other cause because they don?t get the same reactions. My biggest concern is that despite the letter of my doctor and what ocurred in the past weeks with the nurse I am still feeling pressured by my coworkers to work with the chemicals as they feel that a mask, a lab coat and gloves is enough protection. I am unsure about this. I didn?t get a proper fit test for my respirator by the way. I have worked for another corporation where they did that right after getting hired. I have read that chemicals can be absorbed through the skin too. I just want to know the opinion of pregnant lab techs and supervisors who have worked with them. I have read older threads about this in this forum before and everybody had positive and negative experiences. Some workers were completely removed from the lab while others kept performing the same tasks. Some say their babies turned out healthy while others blame the job for causing short and long term health issues for the babies. Most of the employers protected the pregnant worker from the chemicals to avoid any risks which I feel that?s the direction my employer should take. There are 3 other histotechs in the lab and they don?t seem happy to have that extra task in their hands, despite being the one who changed the processor most of the time this past year besides the supervisor. Thank you for your help. This has caused a lot of distress in me and I just want to be safe. From histology400 at gmail.com Sat Jan 18 11:38:46 2020 From: histology400 at gmail.com (Valerie Laughlin) Date: Sat, 18 Jan 2020 09:38:46 -0800 Subject: [Histonet] Pregnant in histo lab. Am I safe? In-Reply-To: References: Message-ID: Yes, going to HR might be my next step before I consider other options. The supervisor bough a respirator with generic filters without knowing he had to buy specific filters for xylene and formalin. We also have regular latex gloves that are definitely not resistant to xylene. Xylene melts the gloves away. On Saturday, January 18, 2020, Jessica Phillips wrote: > I think you need to go to HR and find out what your options are. In the > meantime, you need a respirator, specifically with a filter for xylene and > a separate filter for formalin. You will also need some xylene resistant > gloves with the long cuffs. > > -Jess > > On Sat, Jan 18, 2020, 4:31 AM Valerie Laughlin via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Hello everyone. I am currently in the last weeks of my first trimester of >> my pregnancy. >> >> >> I have asked this question to my Ob-Gyn, family and general pregnancy >> forums but I wanted to ask people who understand the field of >> Histotechnology better. >> >> >> I have been very concerned about the side effects of the chemicals that >> might have on my baby. The lab works with the typical stuff >> (formaldehyde, >> xylene, alcohol of different percentages, glacial acetic acid, stains etc) >> They make the fixative from scratch. >> >> >> I had to inform my supervisor and manager. I didn?t get the most positive >> reaction from them but I don?t care as this is my personal business and I >> have rights like everybody else. >> >> >> I gave them a letter from my doctor informing my pregnancy and that I >> should be kept away from the chemicals for my own safety. >> >> >> They acknowledged the letter but still decided to buy a respirator mask >> for >> me which is fine. It?s good to have protective equipment no matter the >> circumstance. >> >> >> I told them that I can do the same tasks I do every day such as grossing >> but with a mask, embedding, cutting and filing but that I don?t feel >> comfortable changing the chemicals of the tissue processor and slide >> stainer, and mixing chemicals. Also that I can?t dump the chemicals in the >> biohazard room as there is not enough ventilation. >> >> >> Literally an hour after I informed this a nurse who was working in a rojom >> close to the biohazard room had a negative reaction and had to be sent to >> the ER where she was there for days. She blamed the chemicals from the >> biohazard room. Other nurses who work close to that room had reported >> negative side effects as well. This situation made me more uncomfortable >> specially when my coworkers think the nurses are over reacting and it has >> to be some other cause because they don?t get the same reactions. >> >> >> My biggest concern is that despite the letter of my doctor and what >> ocurred >> in the past weeks with the nurse I am still feeling pressured by my >> coworkers to work with the chemicals as they feel that a mask, a lab coat >> and gloves is enough protection. I am unsure about this. >> >> >> I didn?t get a proper fit test for my respirator by the way. I have worked >> for another corporation where they did that right after getting hired. >> >> >> I have read that chemicals can be absorbed through the skin too. >> >> >> I just want to know the opinion of pregnant lab techs and supervisors who >> have worked with them. >> >> >> I have read older threads about this in this forum before and everybody >> had >> positive and negative experiences. Some workers were completely removed >> from the lab while others kept performing the same tasks. Some say their >> babies turned out healthy while others blame the job for causing short and >> long term >> >> health issues for the babies. >> >> >> Most of the employers protected the pregnant worker from the chemicals to >> avoid any risks which I feel that?s the direction my employer should take. >> There are 3 other histotechs in the lab and they don?t seem happy to have >> that extra task in their hands, despite being the one who changed the >> processor most of the time this past year besides the supervisor. >> >> >> Thank you for your help. This has caused a lot of distress in me and I >> just >> want to be safe. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From histology400 at gmail.com Sat Jan 18 12:01:49 2020 From: histology400 at gmail.com (Val L) Date: Sat, 18 Jan 2020 10:01:49 -0800 Subject: [Histonet] Pregnant in histo lab. Am I safe? In-Reply-To: <4ED8C96A8F20FC4F883A92E2A0A0D64AA7F68175@DH-MAIL02.dhorg.org> References: <4ED8C96A8F20FC4F883A92E2A0A0D64AA7F68175@DH-MAIL02.dhorg.org> Message-ID: Sadly I have already been exposed to xylene several times as I cannot avoid the smell. It?s everywhere. There are not enough vents in the lab. I don?t know if it?s ignorance or malice but my manager and coworkers are not quite informed about the dangers that a pregnant woman face in a histology lab. They feel that if the lab passed a xylene vapor tests and give me a general purpose respirator then that?s enough for me to be safe and I can do the same work as everybody else. There is a negligent attitude regarding safety in this laboratory. Also there has been a negative attitude towards pregnant women like if they were are a burden in the lab. It makes me nervous to work here. I don?t think is a healthy work environment. On Saturday, January 18, 2020, Eck, Allison wrote: > Valerie > I have worked in histo with both of my pregnancies with my most recent one > just three months ago. Embedding and cutting and even grossing are fine to > do while pregnant. Under no condition, even with Ppe, should you be > changing stainers or processors or dumping waste or mixing chemicals. A > pregnant woman should not be near powder chemicals as they are inhalation > hazards and xylene in general is an absolute no no. It is a reproductive > toxin and you should have no contact with it. > Please reach out if you have any other questions but your employer mst > make accommodations for you while you are pregnant. > > Allison > > Allison Eck HTL(ASCP)cm, QLS > ________________________________________ > From: Valerie Laughlin via Histonet [histonet at lists.utsouthwestern.edu] > Sent: Saturday, January 18, 2020 7:21 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Pregnant in histo lab. Am I safe? > > Hello everyone. I am currently in the last weeks of my first trimester of > my pregnancy. > > > I have asked this question to my Ob-Gyn, family and general pregnancy > forums but I wanted to ask people who understand the field of > Histotechnology better. > > > I have been very concerned about the side effects of the chemicals that > might have on my baby. The lab works with the typical stuff (formaldehyde, > xylene, alcohol of different percentages, glacial acetic acid, stains etc) > They make the fixative from scratch. > > > I had to inform my supervisor and manager. I didn?t get the most positive > reaction from them but I don?t care as this is my personal business and I > have rights like everybody else. > > > I gave them a letter from my doctor informing my pregnancy and that I > should be kept away from the chemicals for my own safety. > > > They acknowledged the letter but still decided to buy a respirator mask for > me which is fine. It?s good to have protective equipment no matter the > circumstance. > > > I told them that I can do the same tasks I do every day such as grossing > but with a mask, embedding, cutting and filing but that I don?t feel > comfortable changing the chemicals of the tissue processor and slide > stainer, and mixing chemicals. Also that I can?t dump the chemicals in the > biohazard room as there is not enough ventilation. > > > Literally an hour after I informed this a nurse who was working in a rojom > close to the biohazard room had a negative reaction and had to be sent to > the ER where she was there for days. She blamed the chemicals from the > biohazard room. Other nurses who work close to that room had reported > negative side effects as well. This situation made me more uncomfortable > specially when my coworkers think the nurses are over reacting and it has > to be some other cause because they don?t get the same reactions. > > > My biggest concern is that despite the letter of my doctor and what ocurred > in the past weeks with the nurse I am still feeling pressured by my > coworkers to work with the chemicals as they feel that a mask, a lab coat > and gloves is enough protection. I am unsure about this. > > > I didn?t get a proper fit test for my respirator by the way. I have worked > for another corporation where they did that right after getting hired. > > > I have read that chemicals can be absorbed through the skin too. > > > I just want to know the opinion of pregnant lab techs and supervisors who > have worked with them. > > > I have read older threads about this in this forum before and everybody had > positive and negative experiences. Some workers were completely removed > from the lab while others kept performing the same tasks. Some say their > babies turned out healthy while others blame the job for causing short and > long term > > health issues for the babies. > > > Most of the employers protected the pregnant worker from the chemicals to > avoid any risks which I feel that?s the direction my employer should take. > There are 3 other histotechs in the lab and they don?t seem happy to have > that extra task in their hands, despite being the one who changed the > processor most of the time this past year besides the supervisor. > > > Thank you for your help. This has caused a lot of distress in me and I just > want to be safe. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern. > edu/mailman/listinfo/histonet__;!!DSOD-gJhEGKx5ylYJgQ! > wXUhrTNGAT1e7WIrWBzKQma8r8eNl-9C7c6-gVfAyafbTwi2nuEg6Fjh6g$ > From steve8438 at gmail.com Sat Jan 18 13:13:28 2020 From: steve8438 at gmail.com (Steven Mello) Date: Sat, 18 Jan 2020 14:13:28 -0500 Subject: [Histonet] Pregnant in histo lab. Am I safe? In-Reply-To: References: Message-ID: <7D28A7EB-95BD-4FF8-8340-8BFA69882600@gmail.com> Val, It is clearly not, I repeat NOT acceptable to have you exposed to xylene, any powdered chemicals while you are pregnant. Changing of processors, stainers, dehydration or rehydration stations is an absolute No! Anytime one of my staff notified me that they were pregnant the most I let them do was cut, file. Sounds, like a severe OSHA compliance issue! Steven Mello, HT(ASCP) Sent from my iPhone > On Jan 18, 2020, at 1:15 PM, Val L via Histonet wrote: > > ?Sadly I have already been exposed to xylene several times as I cannot avoid > the smell. It?s everywhere. There are not enough vents in the lab. I don?t > know if it?s ignorance or malice but my manager and coworkers are not quite > informed about the dangers that a pregnant woman face in a histology lab. > They feel that if the lab passed a xylene vapor tests and give me a general > purpose respirator then that?s enough for me to be safe and I can do the > same work as everybody else. There is a negligent attitude regarding safety > in this laboratory. Also there has been a negative attitude towards > pregnant women like if they were are a burden in the lab. It makes me > nervous to work here. I don?t think is a healthy work environment. > >> On Saturday, January 18, 2020, Eck, Allison wrote: >> >> Valerie >> I have worked in histo with both of my pregnancies with my most recent one >> just three months ago. Embedding and cutting and even grossing are fine to >> do while pregnant. Under no condition, even with Ppe, should you be >> changing stainers or processors or dumping waste or mixing chemicals. A >> pregnant woman should not be near powder chemicals as they are inhalation >> hazards and xylene in general is an absolute no no. It is a reproductive >> toxin and you should have no contact with it. >> Please reach out if you have any other questions but your employer mst >> make accommodations for you while you are pregnant. >> >> Allison >> >> Allison Eck HTL(ASCP)cm, QLS >> ________________________________________ >> From: Valerie Laughlin via Histonet [histonet at lists.utsouthwestern.edu] >> Sent: Saturday, January 18, 2020 7:21 AM >> To: histonet at lists.utsouthwestern.edu >> Subject: [Histonet] Pregnant in histo lab. Am I safe? >> >> Hello everyone. I am currently in the last weeks of my first trimester of >> my pregnancy. >> >> >> I have asked this question to my Ob-Gyn, family and general pregnancy >> forums but I wanted to ask people who understand the field of >> Histotechnology better. >> >> >> I have been very concerned about the side effects of the chemicals that >> might have on my baby. The lab works with the typical stuff (formaldehyde, >> xylene, alcohol of different percentages, glacial acetic acid, stains etc) >> They make the fixative from scratch. >> >> >> I had to inform my supervisor and manager. I didn?t get the most positive >> reaction from them but I don?t care as this is my personal business and I >> have rights like everybody else. >> >> >> I gave them a letter from my doctor informing my pregnancy and that I >> should be kept away from the chemicals for my own safety. >> >> >> They acknowledged the letter but still decided to buy a respirator mask for >> me which is fine. It?s good to have protective equipment no matter the >> circumstance. >> >> >> I told them that I can do the same tasks I do every day such as grossing >> but with a mask, embedding, cutting and filing but that I don?t feel >> comfortable changing the chemicals of the tissue processor and slide >> stainer, and mixing chemicals. Also that I can?t dump the chemicals in the >> biohazard room as there is not enough ventilation. >> >> >> Literally an hour after I informed this a nurse who was working in a rojom >> close to the biohazard room had a negative reaction and had to be sent to >> the ER where she was there for days. She blamed the chemicals from the >> biohazard room. Other nurses who work close to that room had reported >> negative side effects as well. This situation made me more uncomfortable >> specially when my coworkers think the nurses are over reacting and it has >> to be some other cause because they don?t get the same reactions. >> >> >> My biggest concern is that despite the letter of my doctor and what ocurred >> in the past weeks with the nurse I am still feeling pressured by my >> coworkers to work with the chemicals as they feel that a mask, a lab coat >> and gloves is enough protection. I am unsure about this. >> >> >> I didn?t get a proper fit test for my respirator by the way. I have worked >> for another corporation where they did that right after getting hired. >> >> >> I have read that chemicals can be absorbed through the skin too. >> >> >> I just want to know the opinion of pregnant lab techs and supervisors who >> have worked with them. >> >> >> I have read older threads about this in this forum before and everybody had >> positive and negative experiences. Some workers were completely removed >> from the lab while others kept performing the same tasks. Some say their >> babies turned out healthy while others blame the job for causing short and >> long term >> >> health issues for the babies. >> >> >> Most of the employers protected the pregnant worker from the chemicals to >> avoid any risks which I feel that?s the direction my employer should take. >> There are 3 other histotechs in the lab and they don?t seem happy to have >> that extra task in their hands, despite being the one who changed the >> processor most of the time this past year besides the supervisor. >> >> >> Thank you for your help. This has caused a lot of distress in me and I just >> want to be safe. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://urldefense.com/v3/__http://lists.utsouthwestern. >> edu/mailman/listinfo/histonet__;!!DSOD-gJhEGKx5ylYJgQ! >> wXUhrTNGAT1e7WIrWBzKQma8r8eNl-9C7c6-gVfAyafbTwi2nuEg6Fjh6g$ >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From elaineahoffman55 at yahoo.com Sat Jan 18 15:26:55 2020 From: elaineahoffman55 at yahoo.com (Elaine allison Hoffman) Date: Sat, 18 Jan 2020 21:26:55 +0000 (UTC) Subject: [Histonet] Coverslipper In-Reply-To: References: Message-ID: <1236363288.9060548.1579382815789@mail.yahoo.com> On Thursday, January 16, 2020, 04:05:46 PM EST, Margaryan, Naira via Histonet wrote: Happy Thursday, We would like to sell our slightly used but in excellent condition Dako Coverslipper which was purchased in 2010 (purchase price was $ 25K). The instrument can handle up to 600 slides per hour making it one of the fastest on the market. In addition to the flexibility of the Coverslipper it is easy and straightforward to operate and cleaning and maintenance is simple to do. It is small enough to fit into fume cabinets, easy to move around and accepts a variety of commercial mounting media. Picture by request. Will take any offer, Naira _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor_tobias at comcast.net Sat Jan 18 16:58:27 2020 From: victor_tobias at comcast.net (Victor) Date: Sat, 18 Jan 2020 14:58:27 -0800 Subject: [Histonet] Pregnant in histo lab. Am I safe? In-Reply-To: References: <4ED8C96A8F20FC4F883A92E2A0A0D64AA7F68175@DH-MAIL02.dhorg.org> Message-ID: When I was working in the lab, anyone that was pregnant was automatically removed from changing the processor and other duties where exposure was possible. The other techs had no problem with this as we didn?t have to file slides and blocks for awhile. Don?t forget, HR is there for the employer, not the employee. Victor Sent from Mail for Windows 10 From: Val L via Histonet Sent: Saturday, January 18, 2020 10:02 AM To: Eck, Allison; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pregnant in histo lab. Am I safe? Sadly I have already been exposed to xylene several times as I cannot avoid the smell. It?s everywhere. There are not enough vents in the lab. I don?t know if it?s ignorance or malice but my manager and coworkers are not quite informed about the dangers that a pregnant woman face in a histology lab. They feel that if the lab passed a xylene vapor tests and give me a general purpose respirator then that?s enough for me to be safe and I can do the same work as everybody else. There is a negligent attitude regarding safety in this laboratory. Also there has been a negative attitude towards pregnant women like if they were are a burden in the lab. It makes me nervous to work here. I don?t think is a healthy work environment. On Saturday, January 18, 2020, Eck, Allison wrote: > Valerie > I have worked in histo with both of my pregnancies with my most recent one > just three months ago. Embedding and cutting and even grossing are fine to > do while pregnant. Under no condition, even with Ppe, should you be > changing stainers or processors or dumping waste or mixing chemicals. A > pregnant woman should not be near powder chemicals as they are inhalation > hazards and xylene in general is an absolute no no. It is a reproductive > toxin and you should have no contact with it. > Please reach out if you have any other questions but your employer mst > make accommodations for you while you are pregnant. > > Allison > > Allison Eck HTL(ASCP)cm, QLS > ________________________________________ > From: Valerie Laughlin via Histonet [histonet at lists.utsouthwestern.edu] > Sent: Saturday, January 18, 2020 7:21 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Pregnant in histo lab. Am I safe? > > Hello everyone. I am currently in the last weeks of my first trimester of > my pregnancy. > > > I have asked this question to my Ob-Gyn, family and general pregnancy > forums but I wanted to ask people who understand the field of > Histotechnology better. > > > I have been very concerned about the side effects of the chemicals that > might have on my baby. The lab works with the typical stuff (formaldehyde, > xylene, alcohol of different percentages, glacial acetic acid, stains etc) > They make the fixative from scratch. > > > I had to inform my supervisor and manager. I didn?t get the most positive > reaction from them but I don?t care as this is my personal business and I > have rights like everybody else. > > > I gave them a letter from my doctor informing my pregnancy and that I > should be kept away from the chemicals for my own safety. > > > They acknowledged the letter but still decided to buy a respirator mask for > me which is fine. It?s good to have protective equipment no matter the > circumstance. > > > I told them that I can do the same tasks I do every day such as grossing > but with a mask, embedding, cutting and filing but that I don?t feel > comfortable changing the chemicals of the tissue processor and slide > stainer, and mixing chemicals. Also that I can?t dump the chemicals in the > biohazard room as there is not enough ventilation. > > > Literally an hour after I informed this a nurse who was working in a rojom > close to the biohazard room had a negative reaction and had to be sent to > the ER where she was there for days. She blamed the chemicals from the > biohazard room. Other nurses who work close to that room had reported > negative side effects as well. This situation made me more uncomfortable > specially when my coworkers think the nurses are over reacting and it has > to be some other cause because they don?t get the same reactions. > > > My biggest concern is that despite the letter of my doctor and what ocurred > in the past weeks with the nurse I am still feeling pressured by my > coworkers to work with the chemicals as they feel that a mask, a lab coat > and gloves is enough protection. I am unsure about this. > > > I didn?t get a proper fit test for my respirator by the way. I have worked > for another corporation where they did that right after getting hired. > > > I have read that chemicals can be absorbed through the skin too. > > > I just want to know the opinion of pregnant lab techs and supervisors who > have worked with them. > > > I have read older threads about this in this forum before and everybody had > positive and negative experiences. Some workers were completely removed > from the lab while others kept performing the same tasks. Some say their > babies turned out healthy while others blame the job for causing short and > long term > > health issues for the babies. > > > Most of the employers protected the pregnant worker from the chemicals to > avoid any risks which I feel that?s the direction my employer should take. > There are 3 other histotechs in the lab and they don?t seem happy to have > that extra task in their hands, despite being the one who changed the > processor most of the time this past year besides the supervisor. > > > Thank you for your help. This has caused a lot of distress in me and I just > want to be safe. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern. > edu/mailman/listinfo/histonet__;!!DSOD-gJhEGKx5ylYJgQ! > wXUhrTNGAT1e7WIrWBzKQma8r8eNl-9C7c6-gVfAyafbTwi2nuEg6Fjh6g$ > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ana.Maluenda at baker.edu.au Sat Jan 18 20:38:21 2020 From: Ana.Maluenda at baker.edu.au (Ana Maluenda) Date: Sun, 19 Jan 2020 02:38:21 +0000 Subject: [Histonet] Pregnant in histo lab. Am I safe? In-Reply-To: References: , Message-ID: Hi Valerie, I don't know how it is in US, but in Australia what I did was to liaise with our Occupational Health and Safety Department to check which measurements they'd recommend about having pregnant workers around the lab. My lab however is not heavily Histology and the tissue processing is done off-site, so the exposure to chemicals like Xylene and Formalin is not as much as probably for you as a Histotech. But regardless, they helped with general queries and PPE recommendations (especially fitting respirators). Hope you find a good solution for both sides. Kind regards, Ana Ana Maluenda Research Assistant/Laboratory Manager Atherothrombosis and Vascular Biology Laboratory Baker Heart and Diabetes Institute 75 Commercial Road, Melbourne VIC 3004 ________________________________________ From: Valerie Laughlin [histology400 at gmail.com] Sent: Sunday, 19 January 2020 4:38 AM To: Jessica Phillips; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pregnant in histo lab. Am I safe? Yes, going to HR might be my next step before I consider other options. The supervisor bough a respirator with generic filters without knowing he had to buy specific filters for xylene and formalin. We also have regular latex gloves that are definitely not resistant to xylene. Xylene melts the gloves away. On Saturday, January 18, 2020, Jessica Phillips wrote: > I think you need to go to HR and find out what your options are. In the > meantime, you need a respirator, specifically with a filter for xylene and > a separate filter for formalin. You will also need some xylene resistant > gloves with the long cuffs. > > -Jess > > On Sat, Jan 18, 2020, 4:31 AM Valerie Laughlin via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Hello everyone. I am currently in the last weeks of my first trimester of >> my pregnancy. >> >> >> I have asked this question to my Ob-Gyn, family and general pregnancy >> forums but I wanted to ask people who understand the field of >> Histotechnology better. >> >> >> I have been very concerned about the side effects of the chemicals that >> might have on my baby. The lab works with the typical stuff >> (formaldehyde, >> xylene, alcohol of different percentages, glacial acetic acid, stains etc) >> They make the fixative from scratch. >> >> >> I had to inform my supervisor and manager. I didn?t get the most positive >> reaction from them but I don?t care as this is my personal business and I >> have rights like everybody else. >> >> >> I gave them a letter from my doctor informing my pregnancy and that I >> should be kept away from the chemicals for my own safety. >> >> >> They acknowledged the letter but still decided to buy a respirator mask >> for >> me which is fine. It?s good to have protective equipment no matter the >> circumstance. >> >> >> I told them that I can do the same tasks I do every day such as grossing >> but with a mask, embedding, cutting and filing but that I don?t feel >> comfortable changing the chemicals of the tissue processor and slide >> stainer, and mixing chemicals. Also that I can?t dump the chemicals in the >> biohazard room as there is not enough ventilation. >> >> >> Literally an hour after I informed this a nurse who was working in a rojom >> close to the biohazard room had a negative reaction and had to be sent to >> the ER where she was there for days. She blamed the chemicals from the >> biohazard room. Other nurses who work close to that room had reported >> negative side effects as well. This situation made me more uncomfortable >> specially when my coworkers think the nurses are over reacting and it has >> to be some other cause because they don?t get the same reactions. >> >> >> My biggest concern is that despite the letter of my doctor and what >> ocurred >> in the past weeks with the nurse I am still feeling pressured by my >> coworkers to work with the chemicals as they feel that a mask, a lab coat >> and gloves is enough protection. I am unsure about this. >> >> >> I didn?t get a proper fit test for my respirator by the way. I have worked >> for another corporation where they did that right after getting hired. >> >> >> I have read that chemicals can be absorbed through the skin too. >> >> >> I just want to know the opinion of pregnant lab techs and supervisors who >> have worked with them. >> >> >> I have read older threads about this in this forum before and everybody >> had >> positive and negative experiences. Some workers were completely removed >> from the lab while others kept performing the same tasks. Some say their >> babies turned out healthy while others blame the job for causing short and >> long term >> >> health issues for the babies. >> >> >> Most of the employers protected the pregnant worker from the chemicals to >> avoid any risks which I feel that?s the direction my employer should take. >> There are 3 other histotechs in the lab and they don?t seem happy to have >> that extra task in their hands, despite being the one who changed the >> processor most of the time this past year besides the supervisor. >> >> >> Thank you for your help. This has caused a lot of distress in me and I >> just >> want to be safe. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting privacy at baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg From BMolinari at texasheart.org Mon Jan 20 06:56:20 2020 From: BMolinari at texasheart.org (Betsy Molinari) Date: Mon, 20 Jan 2020 12:56:20 +0000 Subject: [Histonet] Deparaffinized slides References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.381f1c62-12a8-4f37-9cdd-adad10c9e834@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.132adb70-234e-4115-bb23-4ca521a9dd67@emailsignatures365.codetwo.com> Message-ID: Good day and Happy Martin Luther King Day. What are your thoughts on letting deparaffinized slides dry to be used for future use , or keep in DI water for a time? I deparaffinized some slides this morning, then the researcher called and asked me to hold off on staining them. I am going to continue staining them after explaining the situation to her. But I am just curious if they can be held after being deparaffinized. Thank you. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology Houston, Texas 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From BMolinari at texasheart.org Mon Jan 20 08:43:20 2020 From: BMolinari at texasheart.org (Betsy Molinari) Date: Mon, 20 Jan 2020 14:43:20 +0000 Subject: [Histonet] Deparaffinized slides In-Reply-To: <798925916.9475747.1579531299207@mail.yahoo.com> References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.381f1c62-12a8-4f37-9cdd-adad10c9e834@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.132adb70-234e-4115-bb23-4ca521a9dd67@emailsignatures365.codetwo.com> <798925916.9475747.1579531299207@mail.yahoo.com> Message-ID: Great! Thank you! Betsy Molinari Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter From: Paula Keene Pierce Sent: Monday, January 20, 2020 8:42 AM To: Betsy Molinari Subject: Re: [Histonet] Deparaffinized slides *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Crystal Mount. Although it is no longer made under this name, Electron Microscopy Sciences has a substitute called Clear Mount. Place a few drops on the wet section(s) and let dry. It is removed with a few changes of water. I use it all the time for AEC, fat stains, and coating slides to ship out to researchers with no deparaffinization setup. Paula Keene Pierce, BS, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Drive Norman, OK 73069 PH 405-759-3953 http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Monday, January 20, 2020, 07:09:59 AM CST, Betsy Molinari via Histonet > wrote: Good day and Happy Martin Luther King Day. What are your thoughts on letting deparaffinized slides dry to be used for future use , or keep in DI water for a time? I deparaffinized some slides this morning, then the researcher called and asked me to hold off on staining them. I am going to continue staining them after explaining the situation to her. But I am just curious if they can be held after being deparaffinized. Thank you. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology Houston, Texas 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook> | twitter> This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at dpspa.com Mon Jan 20 13:43:10 2020 From: criley at dpspa.com (Charles Riley) Date: Mon, 20 Jan 2020 14:43:10 -0500 Subject: [Histonet] Grossing manual examples Message-ID: Is anyone willing to share an example of their grossing manual setup? I am trying to get ours updated as I feel it doesn't adequately provide instruction on how to gross specimens. I would like some examples to show our pathologists what would be helpful so they can modify the current one we have -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From amosbrooks at gmail.com Mon Jan 20 17:59:19 2020 From: amosbrooks at gmail.com (Amos Brooks) Date: Mon, 20 Jan 2020 18:59:19 -0500 Subject: [Histonet] Histonet Digest, Vol 194, Issue 15 In-Reply-To: References: Message-ID: Hi Betsy, I wouldn't do it. It's an unnecessary risk to let them dry out. Better to leave it in distilled water if you absolutely must. Ideal to not deparaffinize in the first place if you can't finish the stain. Dried out sections is just a bad plan. Amos Brooks On Mon, Jan 20, 2020, 1:00 PM > Message: 1 > Date: Mon, 20 Jan 2020 12:56:20 +0000 > From: Betsy Molinari > To: "'Histonet at lists.utsouthwestern.edu'" > > Subject: [Histonet] Deparaffinized slides > Message-ID: > < > SN6PR10MB28955C0F9DCD3FE5C5FDF3FDCE320 at SN6PR10MB2895.namprd10.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > Good day and Happy Martin Luther King Day. What are your thoughts on > letting deparaffinized slides dry to be used for future use , or keep in DI > water for a time? I deparaffinized some slides this morning, then the > researcher called and asked me to hold off on staining them. I am going to > continue staining them after explaining the situation to her. But I am just > curious if they can be held after being deparaffinized. > Thank you. > > Betsy Molinari HT(ASCP) > Texas Heart Institute > Cardiovascular Pathology > Houston, Texas > ***************************************** > From BMolinari at texasheart.org Tue Jan 21 05:35:33 2020 From: BMolinari at texasheart.org (Betsy Molinari) Date: Tue, 21 Jan 2020 11:35:33 +0000 Subject: [Histonet] Histonet Digest, Vol 194, Issue 15 In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.c3e1edc8-7f26-4eb5-a786-f0d3b8ffdff3@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.1ac95977-5ad9-43a0-ae2f-e150c16c6274@emailsignatures365.codetwo.com> Message-ID: Thank you for the reply Amos. I totally agree but the researcher did not inform me until after the deparaffinization was finished. She wanted to hold off on the staining but it was too late and I went ahead with the staining. I was just curious if there was, in fact a procedure to preserve a deparaffinized slide. Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter -----Original Message----- From: Amos Brooks via Histonet Sent: Monday, January 20, 2020 5:59 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15 *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Hi Betsy, I wouldn't do it. It's an unnecessary risk to let them dry out. Better to leave it in distilled water if you absolutely must. Ideal to not deparaffinize in the first place if you can't finish the stain. Dried out sections is just a bad plan. Amos Brooks On Mon, Jan 20, 2020, 1:00 PM > Message: 1 > Date: Mon, 20 Jan 2020 12:56:20 +0000 > From: Betsy Molinari > To: "'Histonet at lists.utsouthwestern.edu'" > > Subject: [Histonet] Deparaffinized slides > Message-ID: > < > SN6PR10MB28955C0F9DCD3FE5C5FDF3FDCE320 at SN6PR10MB2895.namprd10.prod.out > look.com > > > > Content-Type: text/plain; charset="us-ascii" > > Good day and Happy Martin Luther King Day. What are your thoughts on > letting deparaffinized slides dry to be used for future use , or keep > in DI water for a time? I deparaffinized some slides this morning, > then the researcher called and asked me to hold off on staining them. > I am going to continue staining them after explaining the situation to > her. But I am just curious if they can be held after being deparaffinized. > Thank you. > > Betsy Molinari HT(ASCP) > Texas Heart Institute > Cardiovascular Pathology > Houston, Texas > ***************************************** > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=GcQ8xaDLGa-5zMPbj3K35pahQ08SV4dj5Ka_AOrT2sc&s=WShxiyrJyy3oGjhwV0jxCmtB0sXFvS1Q0rBKL4u_bRI&e= This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From BMolinari at texasheart.org Tue Jan 21 06:53:05 2020 From: BMolinari at texasheart.org (Betsy Molinari) Date: Tue, 21 Jan 2020 12:53:05 +0000 Subject: [Histonet] Histonet Digest, Vol 194, Issue 15 In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.c3e1edc8-7f26-4eb5-a786-f0d3b8ffdff3@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.1ac95977-5ad9-43a0-ae2f-e150c16c6274@emailsignatures365.codetwo.com> Message-ID: Charles, thank you for your reply. That is an interesting idea. I think I may have a bit of an experiment to run when I have some time. Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter From: Charles Riley Sent: Tuesday, January 21, 2020 5:55 AM To: Betsy Molinari Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15 *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ I have never done this before but I would assume you could reseal it just like you would a paraffin block by putting some fresh paraffin on top of the section again and allowing it to dry. It would just then need to be reheated and run down to remove the paraffin cover before staining. On Tue, Jan 21, 2020 at 6:50 AM Betsy Molinari via Histonet > wrote: Thank you for the reply Amos. I totally agree but the researcher did not inform me until after the deparaffinization was finished. She wanted to hold off on the staining but it was too late and I went ahead with the staining. I was just curious if there was, in fact a procedure to preserve a deparaffinized slide. Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org > | facebook > | twitter > -----Original Message----- From: Amos Brooks via Histonet > Sent: Monday, January 20, 2020 5:59 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15 *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Hi Betsy, I wouldn't do it. It's an unnecessary risk to let them dry out. Better to leave it in distilled water if you absolutely must. Ideal to not deparaffinize in the first place if you can't finish the stain. Dried out sections is just a bad plan. Amos Brooks On Mon, Jan 20, 2020, 1:00 PM > Message: 1 > Date: Mon, 20 Jan 2020 12:56:20 +0000 > From: Betsy Molinari > > To: "'Histonet at lists.utsouthwestern.edu'" > > > Subject: [Histonet] Deparaffinized slides > Message-ID: > < > SN6PR10MB28955C0F9DCD3FE5C5FDF3FDCE320 at SN6PR10MB2895.namprd10.prod.out > look.com > > > > Content-Type: text/plain; charset="us-ascii" > > Good day and Happy Martin Luther King Day. What are your thoughts on > letting deparaffinized slides dry to be used for future use , or keep > in DI water for a time? I deparaffinized some slides this morning, > then the researcher called and asked me to hold off on staining them. > I am going to continue staining them after explaining the situation to > her. But I am just curious if they can be held after being deparaffinized. > Thank you. > > Betsy Molinari HT(ASCP) > Texas Heart Institute > Cardiovascular Pathology > Houston, Texas > ***************************************** > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=GcQ8xaDLGa-5zMPbj3K35pahQ08SV4dj5Ka_AOrT2sc&s=WShxiyrJyy3oGjhwV0jxCmtB0sXFvS1Q0rBKL4u_bRI&e= This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From relia1 at earthlink.net Tue Jan 21 08:49:43 2020 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 21 Jan 2020 09:49:43 -0500 Subject: [Histonet] RELIA Solutions is offering FREE Resume tune ups and Career Coaching to all Histology Professionals! Message-ID: <003f01d5d06a$04ce5700$0e6b0500$@earthlink.net> Hi Histonetters! Resumes they're not just for job hunting anymore! Did you know it is a great idea to always keep your resume updated? Resumes and CVs are used for many things besides job hunting: Looking at a raise or promotion? Does your resume list all of your accomplishments so that you can present them to your supervisor? Ever thought about giving a presentation or writing an article? A great CV always makes a presentation much more polished. How about just for you? It feels great to see how far you have come in your career and might help with direction for what's next. How about if you are transitioning? Say going back to permanent work from travel or vice versa or want to move into another area of histology. And of course good old fashioned job hunting! Histopeeps Let me help you get your resume tuned up!! It's free of charge as a service to my Histopeeps!! **We also offer Free Career Coaching!!** Here's what you need to do: Reply to this email with I'm in and we can get started. I look forward to assisting you!! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From BMolinari at texasheart.org Tue Jan 21 10:00:22 2020 From: BMolinari at texasheart.org (Betsy Molinari) Date: Tue, 21 Jan 2020 16:00:22 +0000 Subject: [Histonet] Histonet Digest, Vol 194, Issue 15 In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.c3e1edc8-7f26-4eb5-a786-f0d3b8ffdff3@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.1ac95977-5ad9-43a0-ae2f-e150c16c6274@emailsignatures365.codetwo.com> Message-ID: No I did not. But I will keep that in mind if it happens again. Thanks! Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter -----Original Message----- From: Rathborne, Toni Sent: Tuesday, January 21, 2020 8:56 AM To: Betsy Molinari Subject: RE: [Histonet] Histonet Digest, Vol 194, Issue 15 *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Betsy, I realize that they have been stained now, but did you considered running them back through alcohols and xylene and then coverslipping? The cells would then be preserved and you could easily remove the coverglass and stain at a later date. Toni ------------------------------------------------------------------------------------ NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. -----Original Message----- From: Betsy Molinari via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, January 21, 2020 7:53 AM To: Charles Riley; 'Histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15 *** This is an External Email *** Charles, thank you for your reply. That is an interesting idea. I think I may have a bit of an experiment to run when I have some time. Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter From: Charles Riley Sent: Tuesday, January 21, 2020 5:55 AM To: Betsy Molinari Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15 *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ I have never done this before but I would assume you could reseal it just like you would a paraffin block by putting some fresh paraffin on top of the section again and allowing it to dry. It would just then need to be reheated and run down to remove the paraffin cover before staining. On Tue, Jan 21, 2020 at 6:50 AM Betsy Molinari via Histonet > wrote: Thank you for the reply Amos. I totally agree but the researcher did not inform me until after the deparaffinization was finished. She wanted to hold off on the staining but it was too late and I went ahead with the staining. I was just curious if there was, in fact a procedure to preserve a deparaffinized slide. Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org > | facebook > | twitter > -----Original Message----- From: Amos Brooks via Histonet > Sent: Monday, January 20, 2020 5:59 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15 *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Hi Betsy, I wouldn't do it. It's an unnecessary risk to let them dry out. Better to leave it in distilled water if you absolutely must. Ideal to not deparaffinize in the first place if you can't finish the stain. Dried out sections is just a bad plan. Amos Brooks On Mon, Jan 20, 2020, 1:00 PM > Message: 1 > Date: Mon, 20 Jan 2020 12:56:20 +0000 > From: Betsy Molinari > > > To: "'Histonet at lists.utsouthwestern.edu'" > > n.edu>> > Subject: [Histonet] Deparaffinized slides > Message-ID: > < > SN6PR10MB28955C0F9DCD3FE5C5FDF3FDCE320 at SN6PR10MB2895.namprd10.prod.out > prod.out> > look.com d=DwMFaQ&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJ > LzefN3bjagdyUrCioVawjbCC16NNH8&m=5wwoBF-rqq3qoavhQKMOdIzTCUln4iHFsjxxP > GR9Bfo&s=A65vpkkkXUawRC83YPizUWdZcJI2-rmtq1qxbdZHobM&e=> > > > > Content-Type: text/plain; charset="us-ascii" > > Good day and Happy Martin Luther King Day. What are your thoughts on > letting deparaffinized slides dry to be used for future use , or keep > in DI water for a time? I deparaffinized some slides this morning, > then the researcher called and asked me to hold off on staining them. > I am going to continue staining them after explaining the situation to > her. But I am just curious if they can be held after being deparaffinized. > Thank you. > > Betsy Molinari HT(ASCP) > Texas Heart Institute > Cardiovascular Pathology > Houston, Texas > ***************************************** > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=GcQ8xaDLGa-5zMPbj3K35pahQ08SV4dj5Ka_AOrT2sc&s=WShxiyrJyy3oGjhwV0jxCmtB0sXFvS1Q0rBKL4u_bRI&e= This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=p0Bu9RgcZAx01hkG1tJGDgaadnPqItg-8ntFXtGIGqk&s=GgB_0QqIO1phGyhrsy0t5-VgXZcH1YaAdKAlsXERdO8&e= -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=p0Bu9RgcZAx01hkG1tJGDgaadnPqItg-8ntFXtGIGqk&s=GgB_0QqIO1phGyhrsy0t5-VgXZcH1YaAdKAlsXERdO8&e= From Timothy.Morken at ucsf.edu Tue Jan 21 10:23:15 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 21 Jan 2020 16:23:15 +0000 Subject: [Histonet] Pregnant in histo lab. Am I safe? In-Reply-To: References: <4ED8C96A8F20FC4F883A92E2A0A0D64AA7F68175@DH-MAIL02.dhorg.org> Message-ID: The problem with xylene is that the acceptable air level in the lab is 100ppm but humans can detect it by smell at the 5 - 20ppm range. So it seems like it is "everywhere" but it could still be at a very low level. What level is safe for a pregnancy? CDC has some info on this: https://www.cdc.gov/niosh/topics/repro/solvents.html Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Val L via Histonet Sent: Saturday, January 18, 2020 10:02 AM To: Eck, Allison ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pregnant in histo lab. Am I safe? Sadly I have already been exposed to xylene several times as I cannot avoid the smell. It?s everywhere. There are not enough vents in the lab. I don?t know if it?s ignorance or malice but my manager and coworkers are not quite informed about the dangers that a pregnant woman face in a histology lab. They feel that if the lab passed a xylene vapor tests and give me a general purpose respirator then that?s enough for me to be safe and I can do the same work as everybody else. There is a negligent attitude regarding safety in this laboratory. Also there has been a negative attitude towards pregnant women like if they were are a burden in the lab. It makes me nervous to work here. I don?t think is a healthy work environment. On Saturday, January 18, 2020, Eck, Allison wrote: > Valerie > I have worked in histo with both of my pregnancies with my most recent > one just three months ago. Embedding and cutting and even grossing are > fine to do while pregnant. Under no condition, even with Ppe, should > you be changing stainers or processors or dumping waste or mixing > chemicals. A pregnant woman should not be near powder chemicals as > they are inhalation hazards and xylene in general is an absolute no > no. It is a reproductive toxin and you should have no contact with it. > Please reach out if you have any other questions but your employer mst > make accommodations for you while you are pregnant. > > Allison > > Allison Eck HTL(ASCP)cm, QLS > ________________________________________ > From: Valerie Laughlin via Histonet > [histonet at lists.utsouthwestern.edu] > Sent: Saturday, January 18, 2020 7:21 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Pregnant in histo lab. Am I safe? > > Hello everyone. I am currently in the last weeks of my first trimester > of my pregnancy. > > > I have asked this question to my Ob-Gyn, family and general pregnancy > forums but I wanted to ask people who understand the field of > Histotechnology better. > > > I have been very concerned about the side effects of the chemicals > that might have on my baby. The lab works with the typical stuff > (formaldehyde, xylene, alcohol of different percentages, glacial > acetic acid, stains etc) They make the fixative from scratch. > > > I had to inform my supervisor and manager. I didn?t get the most > positive reaction from them but I don?t care as this is my personal > business and I have rights like everybody else. > > > I gave them a letter from my doctor informing my pregnancy and that I > should be kept away from the chemicals for my own safety. > > > They acknowledged the letter but still decided to buy a respirator > mask for me which is fine. It?s good to have protective equipment no > matter the circumstance. > > > I told them that I can do the same tasks I do every day such as > grossing but with a mask, embedding, cutting and filing but that I > don?t feel comfortable changing the chemicals of the tissue processor > and slide stainer, and mixing chemicals. Also that I can?t dump the > chemicals in the biohazard room as there is not enough ventilation. > > > Literally an hour after I informed this a nurse who was working in a > rojom close to the biohazard room had a negative reaction and had to > be sent to the ER where she was there for days. She blamed the > chemicals from the biohazard room. Other nurses who work close to > that room had reported negative side effects as well. This situation > made me more uncomfortable specially when my coworkers think the > nurses are over reacting and it has to be some other cause because they don?t get the same reactions. > > > My biggest concern is that despite the letter of my doctor and what > ocurred in the past weeks with the nurse I am still feeling pressured > by my coworkers to work with the chemicals as they feel that a mask, a > lab coat and gloves is enough protection. I am unsure about this. > > > I didn?t get a proper fit test for my respirator by the way. I have > worked for another corporation where they did that right after getting hired. > > > I have read that chemicals can be absorbed through the skin too. > > > I just want to know the opinion of pregnant lab techs and supervisors > who have worked with them. > > > I have read older threads about this in this forum before and > everybody had positive and negative experiences. Some workers were > completely removed from the lab while others kept performing the same > tasks. Some say their babies turned out healthy while others blame the > job for causing short and long term > > health issues for the babies. > > > Most of the employers protected the pregnant worker from the chemicals > to avoid any risks which I feel that?s the direction my employer should take. > There are 3 other histotechs in the lab and they don?t seem happy to > have that extra task in their hands, despite being the one who changed > the processor most of the time this past year besides the supervisor. > > > Thank you for your help. This has caused a lot of distress in me and I > just want to be safe. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern. > edu/mailman/listinfo/histonet__;!!DSOD-gJhEGKx5ylYJgQ! > wXUhrTNGAT1e7WIrWBzKQma8r8eNl-9C7c6-gVfAyafbTwi2nuEg6Fjh6g$ > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=CisPSKoPRzK3AilgLK5AoVyiz26qYM6bnkMi-wSRkUI&s=suVHCUGP2XY4JTrNxCw7brs1G4L3or7sl2054VwnLVg&e= From BMolinari at texasheart.org Tue Jan 21 12:18:53 2020 From: BMolinari at texasheart.org (Betsy Molinari) Date: Tue, 21 Jan 2020 18:18:53 +0000 Subject: [Histonet] Deparaffinized slides In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.381f1c62-12a8-4f37-9cdd-adad10c9e834@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.132adb70-234e-4115-bb23-4ca521a9dd67@emailsignatures365.codetwo.com> Message-ID: It does sound like fun! Betsy Molinari, HT (ASCP) Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter -----Original Message----- From: Morken, Timothy Sent: Tuesday, January 21, 2020 11:32 AM To: Betsy Molinari Subject: RE: [Histonet] Deparaffinized slides *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Betsy, sounds like an NSH poster project! - do that and then try different stains after different periods of time. But I suspect that storing them without paraffin won't be a problem. They are already fixed so nothing to degrade, except by oxidation. That means that chemical stains will probably work fine (special stains) but they might not be useful for some IHC stains after a while. What you could do, however, is just re-coat them with a thin layer of paraffin over the tissue and then I'll be they would be good for a long time (don't dip the slide - I've done that before and it makes a mess when deparaffinizing them - that is 1,000 times more wax than you usually have on a slide!). Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Betsy Molinari via Histonet Sent: Monday, January 20, 2020 4:56 AM To: 'Histonet at lists.utsouthwestern.edu' Subject: [Histonet] Deparaffinized slides Good day and Happy Martin Luther King Day. What are your thoughts on letting deparaffinized slides dry to be used for future use , or keep in DI water for a time? I deparaffinized some slides this morning, then the researcher called and asked me to hold off on staining them. I am going to continue staining them after explaining the situation to her. But I am just curious if they can be held after being deparaffinized. Thank you. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology Houston, Texas 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=IqNrARdIuWnDCSzNI7ajuSZ9CBawOeZIgWXmO6KuVGY&s=ECDjQXbR_Bo_Ik-3kbKJ5uOam4UYsTZ9b6Sxhp7Q7m0&e= From tbraud at holyredeemer.com Tue Jan 21 14:38:06 2020 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 21 Jan 2020 20:38:06 +0000 Subject: [Histonet] pregnancy and chemical exposure Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B721C0@HRHEX02-HOS.holyredeemer.local> Hi Val - I'm sorry that you feel that your concerns are not being addressed. I am the safety officer for our entire lab as well as the chair on our hospital's Environmental Safety and Hazardous Materials Committee. I do think that Tim has a valid point that Xylene is detectable by smell long before it poses a danger. It can be smelled at 0.8ppm to 40ppm and only becomes unacceptable at 100ppm. You should have access to your most recent exposure tests which should have been performed at the peak of your work exposure process (i.e. using a Short-term exposure value (STEL) measure while changing the processor, or stainer) This is the best look at your actual exposure. If this STEL has not been done, ask for it to be repeated. It is cheap and easy. Additionally, you should be provided with appropriate PPE which include NITRILE gloves that are approved for Chemical use (looks for the label to state "tested and approved for use with Chemotherapy drugs. Those should be sufficient.) The gloves should be changed every 30 minutes of exposure task time, or sooner if the gloves are compromised. You should also have access to air flow studies for your fume hood that show it is performing according to specs. There are no useful studies of xylene exposure and prenatal risk or developmental toxicity. The best overall study can be found at the NIH, but the only ones that specifically address this had too many limitations to be considered useful and didn't even include exposure levels. As a tech, I have been in the field for over 40 years, I can tell you that I can count over 20 pregnancies amongst me and my coworkers at 3 different institutions, with no problems whatsoever, and many of these were in a not-so-great environment as the one I work in now. As a manager, I would expect that if I gave you the provisions that I discussed, (within STEL, appropriate PPE with Nitrile gloves, and an the hood air flow check) that you should be able to perform your chemical related tasks with no problems. My personal advice is the stressing over this problem is not good for you or your baby either. I wish you and your new baby only the best. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Message: 7 Date: Tue, 21 Jan 2020 16:23:15 +0000 From: "Morken, Timothy" To: Val L , "Eck, Allison" Cc: Histonet Subject: Re: [Histonet] Pregnant in histo lab. Am I safe? The problem with xylene is that the acceptable air level in the lab is 100ppm but humans can detect it by smell at the 5 - 20ppm range. So it seems like it is "everywhere" but it could still be at a very low level. What level is safe for a pregnancy? CDC has some info on this: https://www.cdc.gov/niosh/topics/repro/solvents.html Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Val L via Histonet Sadly I have already been exposed to xylene several times as I cannot avoid the smell. It?s everywhere. There are not enough vents in the lab. I don?t know if it?s ignorance or malice but my manager and coworkers are not quite informed about the dangers that a pregnant woman face in a histology lab. They feel that if the lab passed a xylene vapor tests and give me a general purpose respirator then that?s enough for me to be safe and I can do the same work as everybody else. There is a negligent attitude regarding safety in this laboratory. Also there has been a negative attitude towards pregnant women like if they were are a burden in the lab. It makes me nervous to work here. I don?t think is a healthy work environment. On Saturday, January 18, 2020, Eck, Allison wrote: > From: Valerie Laughlin via Histonet > [histonet at lists.utsouthwestern.edu] > Sent: Saturday, January 18, 2020 7:21 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Pregnant in histo lab. Am I safe? > > Hello everyone. I am currently in the last weeks of my first trimester > of my pregnancy. >> I have asked this question to my Ob-Gyn, family and general pregnancy > forums but I wanted to ask people who understand the field of > Histotechnology better. > I have been very concerned about the side effects of the chemicals > that might have on my baby. The lab works with the typical stuff > (formaldehyde, xylene, alcohol of different percentages, glacial > acetic acid, stains etc) They make the fixative from scratch. > I had to inform my supervisor and manager. I didn?t get the most > positive reaction from them but I don?t care as this is my personal > business and I have rights like everybody else. > I gave them a letter from my doctor informing my pregnancy and that I > should be kept away from the chemicals for my own safety. > They acknowledged the letter but still decided to buy a respirator > mask for me which is fine. It?s good to have protective equipment no > matter the circumstance. > I told them that I can do the same tasks I do every day such as > grossing but with a mask, embedding, cutting and filing but that I > don?t feel comfortable changing the chemicals of the tissue processor > and slide stainer, and mixing chemicals. Also that I can?t dump the > chemicals in the biohazard room as there is not enough ventilation. > Literally an hour after I informed this a nurse who was working in a > rojom close to the biohazard room had a negative reaction and had to > be sent to the ER where she was there for days. She blamed the > chemicals from the biohazard room. Other nurses who work close to > that room had reported negative side effects as well. This situation > made me more uncomfortable specially when my coworkers think the > nurses are over reacting and it has to be some other cause because they don?t get the same reactions. > My biggest concern is that despite the letter of my doctor and what > ocurred in the past weeks with the nurse I am still feeling pressured > by my coworkers to work with the chemicals as they feel that a mask, a > lab coat and gloves is enough protection. I am unsure about this. > I didn?t get a proper fit test for my respirator by the way. I have > worked for another corporation where they did that right after getting hired. > I have read that chemicals can be absorbed through the skin too. > I just want to know the opinion of pregnant lab techs and supervisors > who have worked with them. > I have read older threads about this in this forum before and > everybody had positive and negative experiences. Some workers were > completely removed from the lab while others kept performing the same > tasks. Some say their babies turned out healthy while others blame the > job for causing short and long term health issues for the babies. > Most of the employers protected the pregnant worker from the chemicals > to avoid any risks which I feel that?s the direction my employer should take. > There are 3 other histotechs in the lab and they don?t seem happy to > have that extra task in their hands, despite being the one who changed > the processor most of the time this past year besides the supervisor. > Thank you for your help. This has caused a lot of distress in me and I > just want to be safe. From Timothy.Morken at ucsf.edu Wed Jan 22 10:19:11 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 22 Jan 2020 16:19:11 +0000 Subject: [Histonet] grossing manuals Message-ID: Posted for a friend... To my knowledge the best institutional manual is the University of Michigan's Cutting Manual. -Izak Dimenstein From Douglas.Porter at sparrow.org Wed Jan 22 10:42:43 2020 From: Douglas.Porter at sparrow.org (Porter, Douglas) Date: Wed, 22 Jan 2020 16:42:43 +0000 Subject: [Histonet] [EXTERNAL] grossing manuals In-Reply-To: References: Message-ID: Here are three "grossing manuals" I've found helpful: http://pathology.ucla.edu/gross-manual https://www.pathology.med.umich.edu/cutting https://voices.uchicago.edu/grosspathology/ Douglas A. Porter, HT (ASCP) Pathologist Assistant Anatomic Pathology IT Coordinator Sparrow Center for Laboratory Medicine Department of Pathology 3392 Patient Care Drive Lansing, MI 48911 517-371-9481 (phone) 517-371-9540 (fax) douglas.porter at sparrow.org -----Original Message----- From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, January 22, 2020 11:19 AM To: Histonet Subject: [EXTERNAL] [Histonet] grossing manuals Warning: This email originated from outside of Sparrow. Do not click links or open attachments unless you recognize the sender and are expecting the message. ---------------------------------------------------------------------- Posted for a friend... To my knowledge the best institutional manual is the University of Michigan's Cutting Manual. -Izak Dimenstein _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=qgJBLQvENW4Kb9JcrSOXvj11QOUUKGR5N2IUAtns1Jg&r=6pgpgKsLHvt-FitLISss8MQQkPawKdpRw8msCll96Ts&m=svgdrKo7bzvH2SSVPupNdT58CcpLoGZP6gbub2pbA30&s=RFLlKabnUgYw0UtMlO5-Kd3QuHxZpjqjyKS12QYoekM&e= ________________________________ CONFIDENTIALITY NOTICE: This email communication may contain private, confidential, or legally privileged information intended for the sole use of the designated and/or duly authorized recipient(s). If you are not the intended recipient or have received this email in error, please notify the sender immediately by email and permanently delete all copies of this email including all attachments without reading them. If you are the intended recipient, secure the contents in a manner that conforms to all applicable state and/or federal requirements related to privacy and confidentiality of such information. ________________________________ From relia1 at earthlink.net Wed Jan 22 11:10:21 2020 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 22 Jan 2020 12:10:21 -0500 Subject: [Histonet] Can I post this on the histonet? Message-ID: <000001d5d146$d4b734f0$7e259ed0$@earthlink.net> Hi Histonetters! I hope you are having a great week. I have a quick question for the group and the administrators. I have been a member of the histonet for over 15 years and have always just posted my job openings. Given the extreme shortage of histotechs was wondering if it would be ok to post something like this: I am currently working with an ASCP Certified/New York licensed histotech with 4 years of experience. This tech would like to be located in or around Albany or Saratoga County and points north (Vermont even). Please message me for details. Happy New Year!! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From doolee at shands.ufl.edu Wed Jan 22 11:56:03 2020 From: doolee at shands.ufl.edu (Dooley, Elaine) Date: Wed, 22 Jan 2020 17:56:03 +0000 Subject: [Histonet] muscle antibodies Message-ID: <475b926019c44f26b87b5058bdb8a96a@shands.ufl.edu> Hi histonetters, Does anyone know of antibodies that work on Paraffin sections for DYS1, DYS2 ,DYS3, SARC-A ,SARC-B, SARC-D, SARC-G, and Spectrin. I have antibodies that only are recommended for frozen sections. Just wondering if anyone has this working on Paraffin sections. Does anyone have an anti-myophosphoralase antibody, or anti-SDH antibody for frozen sections? Elaine Dooley UF Health Shands Hospital 352-265-0111 ext 72117 From John.Shelton at UTSouthwestern.edu Wed Jan 22 12:46:10 2020 From: John.Shelton at UTSouthwestern.edu (John Shelton) Date: Wed, 22 Jan 2020 18:46:10 +0000 Subject: [Histonet] Can I post this on the histonet? In-Reply-To: <000001d5d146$d4b734f0$7e259ed0$@earthlink.net> References: <000001d5d146$d4b734f0$7e259ed0$@earthlink.net> Message-ID: <1579718770985.43257@UTSouthwestern.edu> Howdy Pam and Fellow Histonet Users, Myself, and the other moderators that I have appointed, have had our hands full with learning the administrative ropes of Histonet for about a week now. More on introductions in a post to come; promise. We have discussed the principles which Linda and her moderators used over the years, with a specific eye towards advertising and keeping the forum beneficial for the many and uncluttered. Regarding recruiting: We believe all are served by postings from a Hospital, Lab, or even a a Recruiter that educate histonet users of occasional formal openings within their organization, but feel the forum would not be well served by allowing individuals to post "in search of opportunity," or throw their resumes up. So... "I have some positions that I need to fill..." OKAY "I am looking to leave my current job, relocate, etc..." NOT OKAY JS John M Shelton Core Operations Manager Histonet List Owner & Moderator UT Southwestern Histo Pathology Core 5323 Harry Hines Blvd Dallas, Texas 75390-8573 (214)648-1451 Visit the Core's website at: http://www.utsouthwestern.edu/labs/histo-pathology/ ________________________________________ From: zzz_Tag_histonet-lists Sent: Wednesday, January 22, 2020 11:10 AM To: zzz_Tag_histonet-lists Subject: [Histonet] Can I post this on the histonet? Hi Histonetters! I hope you are having a great week. I have a quick question for the group and the administrators. I have been a member of the histonet for over 15 years and have always just posted my job openings. Given the extreme shortage of histotechs was wondering if it would be ok to post something like this: I am currently working with an ASCP Certified/New York licensed histotech with 4 years of experience. This tech would like to be located in or around Albany or Saratoga County and points north (Vermont even). Please message me for details. Happy New Year!! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ UT Southwestern Medical Center The future of medicine, today. From relia1 at earthlink.net Wed Jan 22 12:50:12 2020 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 22 Jan 2020 13:50:12 -0500 Subject: [Histonet] Can I post this on the histonet? In-Reply-To: <1579718770985.43257@UTSouthwestern.edu> References: <000001d5d146$d4b734f0$7e259ed0$@earthlink.net> <1579718770985.43257@UTSouthwestern.edu> Message-ID: <000001d5d154$c7ed8db0$57c8a910$@earthlink.net> Thanks John! Happy New Year!! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia -----Original Message----- From: John Shelton [mailto:John.Shelton at UTSouthwestern.edu] Sent: Wednesday, January 22, 2020 1:46 PM To: Pam Barker Cc: zzz_Tag_histonet-lists Subject: Re: [Histonet] Can I post this on the histonet? Howdy Pam and Fellow Histonet Users, Myself, and the other moderators that I have appointed, have had our hands full with learning the administrative ropes of Histonet for about a week now. More on introductions in a post to come; promise. We have discussed the principles which Linda and her moderators used over the years, with a specific eye towards advertising and keeping the forum beneficial for the many and uncluttered. Regarding recruiting: We believe all are served by postings from a Hospital, Lab, or even a a Recruiter that educate histonet users of occasional formal openings within their organization, but feel the forum would not be well served by allowing individuals to post "in search of opportunity," or throw their resumes up. So... "I have some positions that I need to fill..." OKAY "I am looking to leave my current job, relocate, etc..." NOT OKAY JS John M Shelton Core Operations Manager Histonet List Owner & Moderator UT Southwestern Histo Pathology Core 5323 Harry Hines Blvd Dallas, Texas 75390-8573 (214)648-1451 Visit the Core's website at: http://www.utsouthwestern.edu/labs/histo-pathology/ ________________________________________ From: zzz_Tag_histonet-lists Sent: Wednesday, January 22, 2020 11:10 AM To: zzz_Tag_histonet-lists Subject: [Histonet] Can I post this on the histonet? Hi Histonetters! I hope you are having a great week. I have a quick question for the group and the administrators. I have been a member of the histonet for over 15 years and have always just posted my job openings. Given the extreme shortage of histotechs was wondering if it would be ok to post something like this: I am currently working with an ASCP Certified/New York licensed histotech with 4 years of experience. This tech would like to be located in or around Albany or Saratoga County and points north (Vermont even). Please message me for details. Happy New Year!! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ UT Southwestern Medical Center The future of medicine, today. From tbraud at holyredeemer.com Thu Jan 23 08:10:20 2020 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 23 Jan 2020 14:10:20 +0000 Subject: [Histonet] Need a procedure Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B7269B@HRHEX02-HOS.holyredeemer.local> Hi fellow Histonetters - I'm in need of some help, please Background - We currently use agar to capture our scant cell blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used? Thanks in advance. Histotechs rock! Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From john.garratt at ciqc.ca Thu Jan 23 11:51:14 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 23 Jan 2020 17:51:14 +0000 Subject: [Histonet] Need a procedure In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F801C1B7269B@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F801C1B7269B@HRHEX02-HOS.holyredeemer.local> Message-ID: Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin. Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma. The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC. John www.cpqa.ca ??????? Original Message ??????? On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet wrote: > Hi fellow Histonetters - I'm in need of some help, please > Background - We currently use agar to capture our scant cell blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method > Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used? > Thanks in advance. Histotechs rock! > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster at umn.edu Thu Jan 23 12:19:48 2020 From: cforster at umn.edu (Colleen Forster) Date: Thu, 23 Jan 2020 12:19:48 -0600 Subject: [Histonet] Need a procedure In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F801C1B7269B@HRHEX02-HOS.holyredeemer.local> Message-ID: Terri, I agree with John on the use of Histogel. I use it routinely for all the cell blocks and tiny matrix samples that come down and I get excellent results. I do a lot of IHC and the results are good. Respectfully, Colleen On Thu, Jan 23, 2020 at 11:51 AM John Garratt via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Terri, I suggest you use Histogel for block preparation. It works > exceptionally well, it is good for IHC and does not have the pitfalls of > plasma/thrombin. > Plasma/thrombin does work well for cell blocks but you will have to > consider an ethical and safe source for your plasma. > The instructions for using Histogel are in the package insert though I > have one comment. Be careful how you warm the Histogel and use a heat > block. Do NOT use a microwave since there is a tendency to overheat the gel > and you will end up with poor quality IHC. > > John > > > www.cpqa.ca > > ??????? Original Message ??????? > On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hi fellow Histonetters - I'm in need of some help, please > > Background - We currently use agar to capture our scant cell blocks for > processing. I am unfamiliar with the Plasma/Thrombin method of cell block > preparation and am interested in comparing it to our current method > > Request - Could you please send me your procedures for this method, > specifically where you purchase your plasma and thrombin and what species > are used? > > Thanks in advance. Histotechs rock! > > > > Terri L. Braud, HT(ASCP) > > Anatomic Pathology Supervisor > > Laboratory > > Holy Redeemer Hospital > > 1648 Huntingdon Pike > > Meadowbrook, PA 19046 > > ph: 215-938-3689 > > fax: 215-938-3874 > > Care, Comfort, and Heal > > > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 From ajju33 at gmail.com Thu Jan 23 14:13:39 2020 From: ajju33 at gmail.com (Muhammad Azam) Date: Thu, 23 Jan 2020 15:13:39 -0500 Subject: [Histonet] Need a procedure In-Reply-To: References: Message-ID: <5F825780-2700-47CE-89CC-61BA6BD12082@gmail.com> Anybody has validated procedure for histogel Sent from my iPhone > On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet wrote: > > ?Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin. > Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma. > The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC. > > John > > > www.cpqa.ca > > ??????? Original Message ??????? >> On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet wrote: >> >> Hi fellow Histonetters - I'm in need of some help, please >> Background - We currently use agar to capture our scant cell blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method >> Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used? >> Thanks in advance. Histotechs rock! >> >> Terri L. Braud, HT(ASCP) >> Anatomic Pathology Supervisor >> Laboratory >> Holy Redeemer Hospital >> 1648 Huntingdon Pike >> Meadowbrook, PA 19046 >> ph: 215-938-3689 >> fax: 215-938-3874 >> Care, Comfort, and Heal >> >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tammy at surgicalpathlabs.com Thu Jan 23 14:40:54 2020 From: tammy at surgicalpathlabs.com (Tammy de Leon) Date: Thu, 23 Jan 2020 15:40:54 -0500 Subject: [Histonet] looking for HTL's Message-ID: We're looking for full time Histotechnologists! COME GROW WITH US!!! SPL is CAP accredited and offers competitive pay, a comprehensive benefits package including a retirement plan with a match, supplemental Insurance, and SPL pays 100% of the premiums for medical, dental, vision, life and LTD, and an employee assistance program. Join our team! Email resume to tammy at surgicalpathlabs.com . Tammy R. de Leon Human Resources Officer Surgical Pathology Laboratories 8455 66th Street N Pinellas Park, Fl 33781 Office Phone - 727-209-1215 Fax - 727-545-1644 From john.garratt at ciqc.ca Thu Jan 23 15:21:28 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 23 Jan 2020 21:21:28 +0000 Subject: [Histonet] Need a procedure In-Reply-To: <5F825780-2700-47CE-89CC-61BA6BD12082@gmail.com> References: <5F825780-2700-47CE-89CC-61BA6BD12082@gmail.com> Message-ID: <7fXHgTeSCGCIAl1wFr6dalL_3uf7OyC8EuqyxmLSxIn65WhCNMeUSSEKGun_fzHNDrFB8DUn2NCaW9EUYLT346v1qLPIjB64mbuphZZ1zK4=@ciqc.ca> http://www.avantec.fr/content/dam/tfs/SDG/APD/APD%20Documents/Product%20Manuals%20&%20Specifications/Histology%20Equipment%20and%20Supplies/Embedding%20Cassettes%20and%20Molds/92957066-Richard-Allan-Scientific-HistoGel-Instructions-for-Use.pdf The above link will help. www.cpqa.ca ??????? Original Message ??????? On Thursday, January 23, 2020 12:13 PM, Muhammad Azam wrote: > Anybody has validated procedure for histogel > > Sent from my iPhone > > > On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet histonet at lists.utsouthwestern.edu wrote: > > Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin. > > Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma. > > The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC. > > John > > www.cpqa.ca > > ??????? Original Message ??????? > > > > > On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet histonet at lists.utsouthwestern.edu wrote: > > > Hi fellow Histonetters - I'm in need of some help, please > > > Background - We currently use agar to capture our scant cell blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method > > > Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used? > > > Thanks in advance. Histotechs rock! > > > Terri L. Braud, HT(ASCP) > > > Anatomic Pathology Supervisor > > > Laboratory > > > Holy Redeemer Hospital > > > 1648 Huntingdon Pike > > > Meadowbrook, PA 19046 > > > ph: 215-938-3689 > > > fax: 215-938-3874 > > > Care, Comfort, and Heal > > > Histonet mailing list > > > Histonet at lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Thu Jan 23 16:59:50 2020 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 23 Jan 2020 22:59:50 +0000 Subject: [Histonet] Need a procedure In-Reply-To: <7fXHgTeSCGCIAl1wFr6dalL_3uf7OyC8EuqyxmLSxIn65WhCNMeUSSEKGun_fzHNDrFB8DUn2NCaW9EUYLT346v1qLPIjB64mbuphZZ1zK4=@ciqc.ca> References: <5F825780-2700-47CE-89CC-61BA6BD12082@gmail.com> <7fXHgTeSCGCIAl1wFr6dalL_3uf7OyC8EuqyxmLSxIn65WhCNMeUSSEKGun_fzHNDrFB8DUn2NCaW9EUYLT346v1qLPIjB64mbuphZZ1zK4=@ciqc.ca> Message-ID: Hi all, Be careful of using cell block matrix that requires heat to solubilise the matrix (eg agar or other commercial matrixes like Histogel). Adding a heated matrix to unfixed, or even formalin fixed, material can denature some antigens (eg CEA) resulting in a false negative IPX. Unfortunately the importance of heat as a pre-analytical factor in immunohistochemistry is often not appreciated. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: John Garratt via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 24 January 2020 8:21 AM To: Muhammad Azam Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Need a procedure http://www.avantec.fr/content/dam/tfs/SDG/APD/APD%20Documents/Product%20Manuals%20&%20Specifications/Histology%20Equipment%20and%20Supplies/Embedding%20Cassettes%20and%20Molds/92957066-Richard-Allan-Scientific-HistoGel-Instructions-for-Use.pdf The above link will help. www.cpqa.ca ??????? Original Message ??????? On Thursday, January 23, 2020 12:13 PM, Muhammad Azam wrote: > Anybody has validated procedure for histogel > > Sent from my iPhone > > > On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet histonet at lists.utsouthwestern.edu wrote: > > Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin. > > Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma. > > The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC. > > John > > www.cpqa.ca > > ??????? Original Message ??????? > > > > > On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet histonet at lists.utsouthwestern.edu wrote: > > > Hi fellow Histonetters - I'm in need of some help, please > > > Background - We currently use agar to capture our scant cell > > > blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used? > > > Thanks in advance. Histotechs rock! > > > Terri L. Braud, HT(ASCP) > > > Anatomic Pathology Supervisor > > > Laboratory > > > Holy Redeemer Hospital > > > 1648 Huntingdon Pike > > > Meadowbrook, PA 19046 > > > ph: 215-938-3689 > > > fax: 215-938-3874 > > > Care, Comfort, and Heal > > > Histonet mailing list > > > Histonet at lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From garreyf at gmail.com Thu Jan 23 17:41:49 2020 From: garreyf at gmail.com (Garrey Faller) Date: Thu, 23 Jan 2020 18:41:49 -0500 Subject: [Histonet] Need a procedure In-Reply-To: References: Message-ID: <09CCDED7-768A-427A-B854-22D974701C71@gmail.com> Great helpful information. We use plasma /thrombin. It?s easy to use, and we have plenty of access to it from our blood bank. However, we are looking to switch to Gel. Why? 1. If you don?t keep an eye on your plasma, at some point you could see fungi in your specimen with a chance you might think it?s real and not a contaminant. That?s a problem. Don?t keep it for too long in the refrigerator. It?s a great culture medium for fungi. You could freeze small aliquots I guess. 2. Molecular testing on cell blocks: With the increasing use of molecular testing on cell blocks, plasma thrombin method poses a problem. The plasma introduces other peoples DNA into the patients sample.Think about that. Didn?t know about the heat issue with Gel. Important to know. Thanks ! Garrey Sent from my iPhone > On Jan 23, 2020, at 6:12 PM, Tony Henwood (SCHN) via Histonet wrote: > > ?Hi all, > > Be careful of using cell block matrix that requires heat to solubilise the matrix (eg agar or other commercial matrixes like Histogel). > Adding a heated matrix to unfixed, or even formalin fixed, material can denature some antigens (eg CEA) resulting in a false negative IPX. > Unfortunately the importance of heat as a pre-analytical factor in immunohistochemistry is often not appreciated. > > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children?s Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: John Garratt via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Friday, 24 January 2020 8:21 AM > To: Muhammad Azam > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Need a procedure > > http://www.avantec.fr/content/dam/tfs/SDG/APD/APD%20Documents/Product%20Manuals%20&%20Specifications/Histology%20Equipment%20and%20Supplies/Embedding%20Cassettes%20and%20Molds/92957066-Richard-Allan-Scientific-HistoGel-Instructions-for-Use.pdf > > > The above link will help. > > > > www.cpqa.ca > > ??????? Original Message ??????? >> On Thursday, January 23, 2020 12:13 PM, Muhammad Azam wrote: >> >> Anybody has validated procedure for histogel >> >> Sent from my iPhone >> >>>> On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet histonet at lists.utsouthwestern.edu wrote: >>> Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin. >>> Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma. >>> The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC. >>> John >>> www.cpqa.ca >>> ??????? Original Message ??????? >>> >>>> On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet histonet at lists.utsouthwestern.edu wrote: >>>> Hi fellow Histonetters - I'm in need of some help, please >>>> Background - We currently use agar to capture our scant cell >>>> blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used? >>>> Thanks in advance. Histotechs rock! >>>> Terri L. Braud, HT(ASCP) >>>> Anatomic Pathology Supervisor >>>> Laboratory >>>> Holy Redeemer Hospital >>>> 1648 Huntingdon Pike >>>> Meadowbrook, PA 19046 >>>> ph: 215-938-3689 >>>> fax: 215-938-3874 >>>> Care, Comfort, and Heal >>>> Histonet mailing list >>>> Histonet at lists.utsouthwestern.edu >>>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From john.garratt at ciqc.ca Thu Jan 23 18:35:46 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Fri, 24 Jan 2020 00:35:46 +0000 Subject: [Histonet] Need a procedure In-Reply-To: <09CCDED7-768A-427A-B854-22D974701C71@gmail.com> References: <09CCDED7-768A-427A-B854-22D974701C71@gmail.com> Message-ID: Good point regarding the molecular and another good reason to move away from plasma. Plus taking plasma, even outdated plasma, is fraught with ethical and regularity problems which are best to avoid now that ever milliliter of blood product needs to be accounted for. www.cpqa.ca ??????? Original Message ??????? On Thursday, January 23, 2020 3:41 PM, Garrey Faller wrote: > Great helpful information. > We use plasma /thrombin. > It?s easy to use, and we have plenty of access to it from our blood bank. > > However, we are looking to switch to Gel. > > Why? > > 1. If you don?t keep an eye on your plasma, at some point you could see fungi in your specimen with a chance you might think it?s real and not a contaminant. That?s a problem. Don?t keep it for too long in the refrigerator. It?s a great culture medium for fungi. > You could freeze small aliquots I guess. > > 2. Molecular testing on cell blocks: > With the increasing use of molecular testing on cell blocks, plasma thrombin method poses a problem. The plasma introduces other peoples DNA into the patients sample.Think about that. > > Didn?t know about the heat issue with Gel. Important to know. Thanks ! > > Garrey > > Sent from my iPhone > > > > On Jan 23, 2020, at 6:12 PM, Tony Henwood (SCHN) via Histonet histonet at lists.utsouthwestern.edu wrote: > > Hi all, > > Be careful of using cell block matrix that requires heat to solubilise the matrix (eg agar or other commercial matrixes like Histogel). > > Adding a heated matrix to unfixed, or even formalin fixed, material can denature some antigens (eg CEA) resulting in a false negative IPX. > > Unfortunately the importance of heat as a pre-analytical factor in immunohistochemistry is often not appreciated. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > > Principal Scientist, the Children?s Hospital at Westmead > > Adjunct Fellow, School of Medicine, University of Western Sydney > > Tel: 612 9845 3306 > > Fax: 612 9845 3318 > > Pathology Department > > the children's hospital at westmead > > Cnr Hawkesbury Road and Hainsworth Street, Westmead > > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > -----Original Message----- > > From: John Garratt via Histonet [mailto:histonet at lists.utsouthwestern.edu] > > Sent: Friday, 24 January 2020 8:21 AM > > To: Muhammad Azam ajju33 at gmail.com > > Cc: histonet at lists.utsouthwestern.edu > > Subject: Re: [Histonet] Need a procedure > > http://www.avantec.fr/content/dam/tfs/SDG/APD/APD Documents/Product Manuals & Specifications/Histology Equipment and Supplies/Embedding Cassettes and Molds/92957066-Richard-Allan-Scientific-HistoGel-Instructions-for-Use.pdf > > The above link will help. > > www.cpqa.ca > > ??????? Original Message ??????? > > > > > On Thursday, January 23, 2020 12:13 PM, Muhammad Azam ajju33 at gmail.com wrote: > > > Anybody has validated procedure for histogel > > > Sent from my iPhone > > > > > > > > On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet histonet at lists.utsouthwestern.edu wrote: > > > > > Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin. > > > > > Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma. > > > > > The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC. > > > > > John > > > > > www.cpqa.ca > > > > > ??????? Original Message ??????? > > > > > > > > > On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet histonet at lists.utsouthwestern.edu wrote: > > > > > Hi fellow Histonetters - I'm in need of some help, please > > > > > Background - We currently use agar to capture our scant cell > > > > > blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used? > > > > > Thanks in advance. Histotechs rock! > > > > > Terri L. Braud, HT(ASCP) > > > > > Anatomic Pathology Supervisor > > > > > Laboratory > > > > > Holy Redeemer Hospital > > > > > 1648 Huntingdon Pike > > > > > Meadowbrook, PA 19046 > > > > > ph: 215-938-3689 > > > > > fax: 215-938-3874 > > > > > Care, Comfort, and Heal > > > > > Histonet mailing list > > > > > Histonet at lists.utsouthwestern.edu > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > Histonet mailing list > > > > Histonet at lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. > > > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From koellingr at comcast.net Thu Jan 23 19:07:15 2020 From: koellingr at comcast.net (RAY KOELLING) Date: Thu, 23 Jan 2020 17:07:15 -0800 (PST) Subject: [Histonet] Need a procedure In-Reply-To: References: <5F825780-2700-47CE-89CC-61BA6BD12082@gmail.com> <7fXHgTeSCGCIAl1wFr6dalL_3uf7OyC8EuqyxmLSxIn65WhCNMeUSSEKGun_fzHNDrFB8DUn2NCaW9EUYLT346v1qLPIjB64mbuphZZ1zK4=@ciqc.ca> Message-ID: <1275950417.352869.1579828035933@connect.xfinity.com> Tony has great point. I used to use Histogel (research for cell lines but applicable to cell block). Switched to Low-melt agarose because of the temp consideration on unfixed cells. Low-temp is a little misleading. Melts (in oven at over 60 degrees) but COOL IT and remains liquid at 37degrees (body temp) and some do not gel until 26 degrees. RNA/DNA-ase free so molecular is fine. Temp low 37 degrees (when used to mix cells) so no DNA denaturation and proteins fine. That was 10 years ago. Just checked there are all sorts of "low-melt" agarose out there. Ray, Fair Director, Eastern Washington Regional Science and Engineering Fair. > On January 23, 2020 at 2:59 PM "Tony Henwood (SCHN) via Histonet" wrote: > > > Hi all, > > Be careful of using cell block matrix that requires heat to solubilise the matrix (eg agar or other commercial matrixes like Histogel). > Adding a heated matrix to unfixed, or even formalin fixed, material can denature some antigens (eg CEA) resulting in a false negative IPX. > Unfortunately the importance of heat as a pre-analytical factor in immunohistochemistry is often not appreciated. > > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children?s Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: John Garratt via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Friday, 24 January 2020 8:21 AM > To: Muhammad Azam > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Need a procedure > > http://www.avantec.fr/content/dam/tfs/SDG/APD/APD%20Documents/Product%20Manuals%20&%20Specifications/Histology%20Equipment%20and%20Supplies/Embedding%20Cassettes%20and%20Molds/92957066-Richard-Allan-Scientific-HistoGel-Instructions-for-Use.pdf > > > The above link will help. > > > > www.cpqa.ca > > ??????? Original Message ??????? > On Thursday, January 23, 2020 12:13 PM, Muhammad Azam wrote: > > > Anybody has validated procedure for histogel > > > > Sent from my iPhone > > > > > On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet histonet at lists.utsouthwestern.edu wrote: > > > Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin. > > > Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma. > > > The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC. > > > John > > > www.cpqa.ca > > > ??????? Original Message ??????? > > > > > > > On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet histonet at lists.utsouthwestern.edu wrote: > > > > Hi fellow Histonetters - I'm in need of some help, please > > > > Background - We currently use agar to capture our scant cell > > > > blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used? > > > > Thanks in advance. Histotechs rock! > > > > Terri L. Braud, HT(ASCP) > > > > Anatomic Pathology Supervisor > > > > Laboratory > > > > Holy Redeemer Hospital > > > > 1648 Huntingdon Pike > > > > Meadowbrook, PA 19046 > > > > ph: 215-938-3689 > > > > fax: 215-938-3874 > > > > Care, Comfort, and Heal > > > > Histonet mailing list > > > > Histonet at lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > Histonet mailing list > > > Histonet at lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Thu Jan 23 19:36:39 2020 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 24 Jan 2020 01:36:39 +0000 Subject: [Histonet] Need a procedure In-Reply-To: References: <09CCDED7-768A-427A-B854-22D974701C71@gmail.com> Message-ID: <78714f265efc4f1e958935de6fd87b96@SVDCMBX-MEX024.nswhealth.net> Good points but: 1. Good QC of the technique is required. The plasma has been tested for known infectious agents and (in Australia) has been treated to remove/kill these organisms. When we received the expired plasma, usually in a bag, we immediately aliquot to smaller volumes and store frozen. They are defrosted when needed. 2. The issue with molecular testing has not been proven to be an issue (I have read the paper: Balassanian, R., Ng, D. L., & van Zante, A. (2019). Stop using expired plasma for cell blocks. Cancer cytopathology, 127(12), 737-738). If contaminating human DNA is a problem, then I prefer to scrape the material from an alcohol fixed, PAP stained slide rather than a cellblock. In fact the DNA and RNA retrieved is of better quality than from a formalin-fixed cell block anyway (also refer to: Knoepp, S. M., & Roh, M. H. (2013). Ancillary techniques on direct?smear aspirate slides: a significant evolution for cytopathology techniques. Cancer cytopathology, 121(3), 120-128). Also, has anyone assessed the deleterious effect of heat on molecular testing? 3. It needs to be remembered that an accurate diagnosis is the aim of the biopsy. Using the plasma clot method followed by formalin fixation is the same as routine core biopsy fixation, so the expected IPX results should be the same. Molecular testing will only be requested after the correct diagnosis is made. 4. I have followed those researchers that have promoted formal-substitute fixatives as being the bee's knees and undoubtedly morphology, immunohistochemistry and molecular testing are good (sometimes better) than formalin-fixed biopsies BUT I have yet to see a follow-up study on these same tissues that were fixed 5, 10 or more years ago. Is the morphology just as good or, I fear, without the protection of formalin, just mushy! Is the IPX and nucleic acids of the same quality. The lack of these follow-up studies make me worried that maybe this may have been a mistake! 5. I am not sure what the ethical issues are. Surely we are just utilising anonymous, de-identified material that would have been disposed of? Hey, my rant for the year!! -----Original Message----- From: John Garratt [mailto:john.garratt at ciqc.ca] Sent: Friday, 24 January 2020 11:36 AM To: Garrey Faller Cc: Tony Henwood (SCHN) ; Muhammad Azam ; Terri Braud ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Need a procedure Good point regarding the molecular and another good reason to move away from plasma. Plus taking plasma, even outdated plasma, is fraught with ethical and regularity problems which are best to avoid now that ever milliliter of blood product needs to be accounted for. www.cpqa.ca ??????? Original Message ??????? On Thursday, January 23, 2020 3:41 PM, Garrey Faller wrote: > Great helpful information. > We use plasma /thrombin. > It?s easy to use, and we have plenty of access to it from our blood bank. > > However, we are looking to switch to Gel. > > Why? > > 1. If you don?t keep an eye on your plasma, at some point you could see fungi in your specimen with a chance you might think it?s real and not a contaminant. That?s a problem. Don?t keep it for too long in the refrigerator. It?s a great culture medium for fungi. > You could freeze small aliquots I guess. > > 2. Molecular testing on cell blocks: > With the increasing use of molecular testing on cell blocks, plasma thrombin method poses a problem. The plasma introduces other peoples DNA into the patients sample.Think about that. > > Didn?t know about the heat issue with Gel. Important to know. Thanks ! > > Garrey > > Sent from my iPhone > > > > On Jan 23, 2020, at 6:12 PM, Tony Henwood (SCHN) via Histonet histonet at lists.utsouthwestern.edu wrote: > > Hi all, > > Be careful of using cell block matrix that requires heat to solubilise the matrix (eg agar or other commercial matrixes like Histogel). > > Adding a heated matrix to unfixed, or even formalin fixed, material can denature some antigens (eg CEA) resulting in a false negative IPX. > > Unfortunately the importance of heat as a pre-analytical factor in immunohistochemistry is often not appreciated. > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > > Principal Scientist, the Children?s Hospital at Westmead Adjunct > > Fellow, School of Medicine, University of Western Sydney > > Tel: 612 9845 3306 > > Fax: 612 9845 3318 > > Pathology Department > > the children's hospital at westmead > > Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, > > Westmead NSW 2145, AUSTRALIA -----Original Message----- > > From: John Garratt via Histonet > > [mailto:histonet at lists.utsouthwestern.edu] > > Sent: Friday, 24 January 2020 8:21 AM > > To: Muhammad Azam ajju33 at gmail.com > > Cc: histonet at lists.utsouthwestern.edu > > Subject: Re: [Histonet] Need a procedure > > http://www.avantec.fr/content/dam/tfs/SDG/APD/APD Documents/Product > > Manuals & Specifications/Histology Equipment and Supplies/Embedding > > Cassettes and > > Molds/92957066-Richard-Allan-Scientific-HistoGel-Instructions-for-Us > > e.pdf > > The above link will help. > > www.cpqa.ca > > ??????? Original Message ??????? > > > > > On Thursday, January 23, 2020 12:13 PM, Muhammad Azam ajju33 at gmail.com wrote: > > > Anybody has validated procedure for histogel Sent from my iPhone > > > > > > > > On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet histonet at lists.utsouthwestern.edu wrote: > > > > > Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin. > > > > > Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma. > > > > > The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC. > > > > > John > > > > > www.cpqa.ca > > > > > ??????? Original Message ??????? > > > > > > > > > On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet histonet at lists.utsouthwestern.edu wrote: > > > > > Hi fellow Histonetters - I'm in need of some help, please > > > > > Background - We currently use agar to capture our scant cell > > > > > blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used? > > > > > Thanks in advance. Histotechs rock! > > > > > Terri L. Braud, HT(ASCP) > > > > > Anatomic Pathology Supervisor > > > > > Laboratory > > > > > Holy Redeemer Hospital > > > > > 1648 Huntingdon Pike > > > > > Meadowbrook, PA 19046 > > > > > ph: 215-938-3689 > > > > > fax: 215-938-3874 > > > > > Care, Comfort, and Heal > > > > > Histonet mailing list > > > > > Histonet at lists.utsouthwestern.edu > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > Histonet mailing list > > > > Histonet at lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. > > > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From jkiernan at uwo.ca Fri Jan 24 00:01:00 2020 From: jkiernan at uwo.ca (John Kiernan) Date: Fri, 24 Jan 2020 06:01:00 +0000 Subject: [Histonet] Question about gelatin embedding In-Reply-To: References: Message-ID: Gelatin embedding is easy. You infiltrate the specimen and then fix it again in formaldehyde to cross-link the gelatin molecules and make the whole mass isoluble in water. You can than cut frozen sections of any kind: cryostat, or with an old-fashioned freezing microtome collecting thawed sections from the knife with a brush. The formaldehyde-fixed gelatin holds everything together. With a Nissl stain it remains inconspicuous. If you do an H&E the gelatin will stain red. John Kiernan = = = ________________________________ From: Alonso Mart?nez Canabal via Histonet Sent: 17 January 2020 16:29 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Question about gelatin embedding Hello, I am here again. I am wondering if someone has good experience embedding in gelatin-albumin for cryostat or vibratome sectioning. Specifically we use brain tissue and is common in free floating techniques non-attached parts of the same section float around and later that generates all sorts of problems. Thank you very much. -- Dr. Alonso Mart?nez Canabal PhD Profesor Asociado "C" Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From richard.wild at sfr.fr Fri Jan 24 00:57:20 2020 From: richard.wild at sfr.fr (richard.wild at sfr.fr) Date: Fri, 24 Jan 2020 07:57:20 +0100 Subject: [Histonet] alternative labels for zebra printer TLP 2742 connected to dako agilent autostainer plus Message-ID: <9d04027c-a6ed-b0d2-157f-98efe150dba2@sfr.fr> Hi the list. Do you know alternativ labels for zebra printer TLP 2742, connected to dako agilent autostainer plus ? Did you try it ? It should have the same size, and same distance between labels, and be resistant to solvant and other chemicals, and thermal printed (instead of Agilent dako autostainer : S341730????????S3417????????Slide Label Kit, Large Flap, 6 rolls per kit, 3000 label) Best Richard From paula at excaliburpathology.com Fri Jan 24 08:31:26 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Fri, 24 Jan 2020 14:31:26 +0000 (UTC) Subject: [Histonet] alternative labels for zebra printer TLP 2742 connected to dako agilent autostainer plus In-Reply-To: <9d04027c-a6ed-b0d2-157f-98efe150dba2@sfr.fr> References: <9d04027c-a6ed-b0d2-157f-98efe150dba2@sfr.fr> Message-ID: <1979617534.11126328.1579876286576@mail.yahoo.com> Hello, I purchase my solvent resistant labels and thermal ribbons for my Zebra printer from Electronic Imaging Materials. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Friday, January 24, 2020, 01:12:38 AM CST, richard.wild--- via Histonet wrote: Hi the list. Do you know alternativ labels for zebra printer TLP 2742, connected to dako agilent autostainer plus ? Did you try it ? It should have the same size, and same distance between labels, and be resistant to solvant and other chemicals, and thermal printed (instead of Agilent dako autostainer : S341730????????S3417????????Slide Label Kit, Large Flap, 6 rolls per kit, 3000 label) Best Richard _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdavoli at gmail.com Fri Jan 24 09:51:49 2020 From: kdavoli at gmail.com (Kate Davoli) Date: Fri, 24 Jan 2020 10:51:49 -0500 Subject: [Histonet] (no subject) Message-ID: I'm all set to DYI my own phosphomolybdic/phosphotungstic acid solution for running a Masson Trichrome, but I see that the former reagent was purchased as "phosphomolybdic acid hydrate". All the recipes I have seen call for just "phosphomolybdic acid" but that is not a reagent that appears to exist without the water molecules coming along for the ride, unless you want to invest in chromatography grade stuff, which I think histology folks probably don't routinely do. The recipes all call for equal gram amounts of each of these crystals, so here's my question: Do I calculate how much weight the water is taking up and add more phosphomolybdic acid crystals (to account for its tagalong water molecules) than called for in the recipe? Or are these recipes already assuming that phosphomolybdic acid HYDRATE is the reagent you have on hand, and I should stick with equal amounts? This question is somewhat complicated by the fact that the molecular formula on the bottle is listed as H3Mo12O40P.XH2O ... which I think means the manufacturer won't bet on exactly how many water molecules are involved. Any advice appreciated! Katherine Davoli, BA, HTL(ASCP)CM Supervisor & Lab Manager, Tissue Culture & Histology Core Module Ophthalmic and Visual Sciences Research Center Department of Ophthalmology, University of Pittsburgh Mail Stop Code: EEI010901 930 Eye & Ear Institute, 203 Lothrop Street Pittsburgh, PA 15213 (412) 647-8256 davolika at upmc.edu and kdavoli at gmail.com From michael.gudo at morphisto.de Sat Jan 25 06:46:10 2020 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Sat, 25 Jan 2020 13:46:10 +0100 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: <70273C83-AB97-4A36-9F08-DC6EE988883C@morphisto.de> Dear Kate, very interesting question. The both substances you mention are a bit difficult in this respect. In a general crystal structure phosphortungstic acid has 44 water molecules and phosphormolybdic acid 28 molecules of crystal water. This would mean, that for a an exact 5% solution you would need around 72,62 g of phosphortungstic acids and 72,73 of phosphormolybdic acid on 1.000 ml water. The standard for a 5 % solution of a solid substance without crystal water would be 52,63 g per 1.000 ml. However, in this case, the substances do not exactly have this number of water molecules in the crystal structure because they loose water and they are hygroscopic. This means that they take more water molecules from the air and that they partly melt in the bottle, if leave it open to long. ?? x H20? - does not mean, that the manufacturers don?t want to give this information, its because it cannot be known exactly. For these substances histological practice is to ignore the crystal water and to use the 52,63 g substance for 1.000 ml of water to get a 5% solution (or to add 5 g substance to 95 g water). This does not have an crucial effect, because the mordanting process for which these acids are used has to be controlled by microscopy anyway. So if you want to make it faster you can add more substance. The important point is only, to make it always the same way. Kind regards Michael > Am 24.01.2020 um 16:51 schrieb Kate Davoli via Histonet : > > I'm all set to DYI my own phosphomolybdic/phosphotungstic acid solution for > running a Masson Trichrome, but I see that the former reagent was > purchased as "phosphomolybdic acid hydrate". > > All the recipes I have seen call for just "phosphomolybdic acid" but that > is not a reagent that appears to exist without the water molecules coming > along for the ride, unless you want to invest in chromatography grade > stuff, which I think histology folks probably don't routinely do. > > The recipes all call for equal gram amounts of each of these crystals, so > here's my question: > > Do I calculate how much weight the water is taking up and add more > phosphomolybdic acid crystals (to account for its tagalong water molecules) > than called for in the recipe? Or are these recipes already assuming that > phosphomolybdic acid HYDRATE is the reagent you have on hand, and I should > stick with equal amounts? > > This question is somewhat complicated by the fact that the molecular > formula on the bottle is listed as H3Mo12O40P.XH2O ... which I think means > the manufacturer won't bet on exactly how many water molecules are involved. > > Any advice appreciated! > > Katherine Davoli, BA, HTL(ASCP)CM > Supervisor & Lab Manager, Tissue Culture & Histology Core Module > Ophthalmic and Visual Sciences Research Center > Department of Ophthalmology, University of Pittsburgh > Mail Stop Code: EEI010901 > 930 Eye & Ear Institute, 203 Lothrop Street > Pittsburgh, PA 15213 > (412) 647-8256 davolika at upmc.edu and kdavoli at gmail.com > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************** MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From Muhammad.Azam2 at va.gov Sun Jan 26 19:45:51 2020 From: Muhammad.Azam2 at va.gov (Azam, Muhammad) Date: Mon, 27 Jan 2020 01:45:51 +0000 Subject: [Histonet] Histochemical autostainer Message-ID: Hi all: Our special stainer went kaput last week. Anybody would recommend a reliable benchtop autostainer for histochemical stains. V/r Muhammad Azam, MD Chief, Pathology & Laboratory Medicine Service (113) James H. Quillen VAMC Mountain Home, TN Phone: 423-979-3529 Cell: 423-741-9872 From bcooper at chla.usc.edu Sun Jan 26 21:59:09 2020 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Mon, 27 Jan 2020 03:59:09 +0000 Subject: [Histonet] Histochemical autostainer In-Reply-To: References: Message-ID: Dako (Agilent) Artisan is the way to go. Thanks, Brian Cooper Histology Supervisor Children's Hospital Los Angeles Sent from my cell phone, so please excuse any funny typos. On Jan 26, 2020 5:47 PM, "Azam, Muhammad via Histonet" wrote: Hi all: Our special stainer went kaput last week. Anybody would recommend a reliable benchtop autostainer for histochemical stains. V/r Muhammad Azam, MD Chief, Pathology & Laboratory Medicine Service (113) James H. Quillen VAMC Mountain Home, TN Phone: 423-979-3529 Cell: 423-741-9872 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From pruegghm at hotmail.com Tue Jan 28 15:31:05 2020 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Tue, 28 Jan 2020 21:31:05 +0000 Subject: [Histonet] Need a procedure In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F801C1B7269B@HRHEX02-HOS.holyredeemer.local>, Message-ID: Terri, when I was in animal research we used the blood from the animal to make plasma and combined it with some thrombin from the hospital pharmacy. Of course that is tedious especially since histogel is available. I warmed the histogel by placing a tube of it in a beaker of hot water, do not mw it. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: John Garratt Sent: Thursday, January 23, 2020 10:51 AM To: Terri Braud Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Need a procedure Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin. Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma. The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC. John www.cpqa.ca ??????? Original Message ??????? On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet wrote: > Hi fellow Histonetters - I'm in need of some help, please > Background - We currently use agar to capture our scant cell blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method > Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used? > Thanks in advance. Histotechs rock! > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 at gmail.com Wed Jan 29 06:24:54 2020 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Wed, 29 Jan 2020 05:24:54 -0700 Subject: [Histonet] Tissue disposal Message-ID: Good morning Histopeeps! After reviewing our Policy Procedure Manual, our manager is questioning the bags we are using for disposing our tissues after diagnosis is complete. We are separating the tissue from the formalin by straining the excess formalin and neutralizing it. We then put the tissue into ?Large Biohazard Bags? and the EVS staff picks it up and disposes it. My manager is wondering if the ?Biohazard Bags" are appropriate. Should the bags state ?Tissue? on the them? Are there any special disposal bags available? While working for a GI Lab in Monterey, CA we put all our bottle and tissue in "Biohazard Bags" and a Hazardous Waste company picked up the waste products from our endoscopy department along with ours. Any comments and suggestions would be greatly appreciated. Thank you in advance, Akemi Allison, BS, HT/HTL From Connie.J.Dieringer at uth.tmc.edu Wed Jan 29 12:54:33 2020 From: Connie.J.Dieringer at uth.tmc.edu (Dieringer, Connie J) Date: Wed, 29 Jan 2020 18:54:33 +0000 Subject: [Histonet] Tissue disposal In-Reply-To: References: Message-ID: <16d93e733e6841569a7059c29f67ef2c@uth.tmc.edu> Eileen, I now work at a smaller facility, but when I worked for a large hospital, we did the same as what you described. We would strain off the formalin and then dispose of the tissue in large biohazard bags and a waste company would come and pick it up. Connie Dieringer, HTL(ASCP) Chief Histologist Department of Diagnostic & Biomedical Science | Oral Pathology Histology Laboratory 7500 Cambridge Street | Suite 6110 | Houston,TX 77054 713-486-4413 tel | 713-486-0415 fax utoralpathology at uth.tmc.edu -----Original Message----- From: Eileen Akemi Allison Sent: Wednesday, January 29, 2020 6:25 AM To: Histonet Cc: MirnaIbarra at umcelpaso.org Subject: [Histonet] Tissue disposal Good morning Histopeeps! After reviewing our Policy Procedure Manual, our manager is questioning the bags we are using for disposing our tissues after diagnosis is complete. We are separating the tissue from the formalin by straining the excess formalin and neutralizing it. We then put the tissue into ?Large Biohazard Bags? and the EVS staff picks it up and disposes it. My manager is wondering if the ?Biohazard Bags" are appropriate. Should the bags state ?Tissue? on the them? Are there any special disposal bags available? While working for a GI Lab in Monterey, CA we put all our bottle and tissue in "Biohazard Bags" and a Hazardous Waste company picked up the waste products from our endoscopy department along with ours. Any comments and suggestions would be greatly appreciated. Thank you in advance, Akemi Allison, BS, HT/HTL From Kelly.Pairan at nationwidechildrens.org Wed Jan 29 13:01:28 2020 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Wed, 29 Jan 2020 19:01:28 +0000 Subject: [Histonet] Germinoma Control Blocks Message-ID: Good Afternoon, We are looking for a few germinoma tissue control blocks. If you happen to have some you can spare, please email me at kelly.pairan at nationwidechildrens.org. We may have something you need and would be happy to do a trade. Thanks, Kelly Kelly Pairan, HT (ASCP)CM, QIHC (ASCP) Histology Supervisor-Anatomic Pathology Department of Pathology and Laboratory Medicine Email: kelly.pairan at nationwidechildrens.org ph: 614-722-5414 fx: 614-722-3033 From cfields at mlkch.org Wed Jan 29 13:05:14 2020 From: cfields at mlkch.org (Carol G Fields) Date: Wed, 29 Jan 2020 19:05:14 +0000 Subject: [Histonet] FW: Tissue disposal In-Reply-To: References: Message-ID: FYI?_____________________________________________ From: Carol G Fields Sent: Wednesday, January 29, 2020 8:32 AM To: 'Eileen Akemi Allison' Subject: RE: [Histonet] Tissue disposal Hi, Stericycle takes care of all your waste, including decanting and disposal. They are all over the US..Health & Safety alone plus employee time will justify you using these people. No one should still be doing this? I?ve used this company at several hospitals. They are great. Good luck, Carole MLKCH Los Angeles, CA https://www.stericycle.com/landing-pages/minimal-global-wrapper/regulated-medical-waste-disposal?utm_campaign=SEM_Ongoing_TopCoreExact&utm_source=Google&utm_medium=cpc&utm_content=B_Top_Core_G_SEM_Txt_Exact&utm_term=stericycle&gclid=CjwKCAiA98TxBRBtEiwAVRLqu8nk2kjv5k4J-d_zb9menIfZRowLKKJY4Df3abYLkdk0xS02OKgFBBoC8k8QAvD_BwE Stericycle is a compliance company that specializes in collecting and disposing regulated substances, such as medical waste and sharps, pharmaceuticals, hazardous waste, and providing services for recalled and expired goods. It also provides related education and training services, and patient communication services. Wikipedia Stock price: SRCL (NASDAQ) $63.65 +0.11 (+0.17%) Jan 29, 10:57 AM EST - Disclaimer Headquarters: Lake Forest, IL CEO: Cindy J. Miller (May 2, 2019?) Number of employees: 23,200 (2017) Founded: 1989 Subsidiaries: Shred-it, Stericycle Environmental Solutions, MORE -----Original Message----- From: Eileen Akemi Allison via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, January 29, 2020 4:25 AM To: Histonet > Cc: MirnaIbarra at umcelpaso.org Subject: [Histonet] Tissue disposal Good morning Histopeeps! After reviewing our Policy Procedure Manual, our manager is questioning the bags we are using for disposing our tissues after diagnosis is complete. We are separating the tissue from the formalin by straining the excess formalin and neutralizing it. We then put the tissue into ?Large Biohazard Bags? and the EVS staff picks it up and disposes it. My manager is wondering if the ?Biohazard Bags" are appropriate. Should the bags state ?Tissue? on the them? Are there any special disposal bags available? While working for a GI Lab in Monterey, CA we put all our bottle and tissue in "Biohazard Bags" and a Hazardous Waste company picked up the waste products from our endoscopy department along with ours. Any comments and suggestions would be greatly appreciated. Thank you in advance, Akemi Allison, BS, HT/HTL _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=zGJiNS11VbY0EhlUMdsr-w&r=JnmrOZ2W3u0Vym5qmaXBgNOijhynn3Nj4f0oLu1UdDo&m=tnwnbsEOJHiPAoYQtnkWynkJVqbIH7NbA32Sp1Btsns&s=_lD3rkVbKauOy3yifxm4MAk5WB-QrqSriKnDM_KV6ik&e= This email message and any files transmitted are sent with confidentiality in mind and contain privileged or copyright information. You must not present this message to another party without gaining permission from the sender. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. Any views expressed in this message are those of the sender, except where the sender specifically states them to be the views of Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. If you have received this message in error, please notify the sender immediately, and delete this email from your system. We do not guarantee that this material is free from viruses or any other defects although due care has been taken to minimize the risk. From Kelly.Pairan at nationwidechildrens.org Wed Jan 29 13:08:35 2020 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Wed, 29 Jan 2020 19:08:35 +0000 Subject: [Histonet] Full-time Job Opening at Nationwide Children's Hospital Message-ID: Good Afternoon Histoland, We are looking for a full-time histotechnician or histotechnologist at Nationwide Children's Hospital. Our lab is open Monday to Friday from 3:30am until 6pm. We rotate on-call Saturdays and Holidays. The job is posted at : https://external-nationwidechildrens.icims.com/jobs/22504/laboratory-technologist---anatomic-pathology/job/. If you have any questions or for a more detailed job description, please email me at kelly.pairan at nationwidechildrens.org or give me a call. Kelly Pairan, HT (ASCP)CM, QIHC (ASCP) Histology Supervisor-Anatomic Pathology Department of Pathology and Laboratory Medicine Email: kelly.pairan at nationwidechildrens.org ph: 614-722-5414 fx: 614-722-3033 From Toni.Rathborne at RWJBH.org Wed Jan 29 13:43:39 2020 From: Toni.Rathborne at RWJBH.org (Rathborne, Toni) Date: Wed, 29 Jan 2020 19:43:39 +0000 Subject: [Histonet] FW: Tissue disposal In-Reply-To: References: Message-ID: ? Stericycle will also take your blocks and slides when you're ready to discard them. Toni ------------------------------------------------------------------------------------ NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. -----Original Message----- From: Carol G Fields via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, January 29, 2020 2:05 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] FW: Tissue disposal *** This is an External Email *** FYI?_____________________________________________ From: Carol G Fields Sent: Wednesday, January 29, 2020 8:32 AM To: 'Eileen Akemi Allison' Subject: RE: [Histonet] Tissue disposal Hi, Stericycle takes care of all your waste, including decanting and disposal. They are all over the US..Health & Safety alone plus employee time will justify you using these people. No one should still be doing this? I?ve used this company at several hospitals. They are great. Good luck, Carole MLKCH Los Angeles, CA https://urldefense.proofpoint.com/v2/url?u=https-3A__www.stericycle.com_landing-2Dpages_minimal-2Dglobal-2Dwrapper_regulated-2Dmedical-2Dwaste-2Ddisposal-3Futm-5Fcampaign-3DSEM-5FOngoing-5FTopCoreExact-26utm-5Fsource-3DGoogle-26utm-5Fmedium-3Dcpc-26utm-5Fcontent-3DB-5FTop-5FCore-5FG-5FSEM-5FTxt-5FExact-26utm-5Fterm-3Dstericycle-26gclid-3DCjwKCAiA98TxBRBtEiwAVRLqu8nk2kjv5k4J-2Dd-5Fzb9menIfZRowLKKJY4Df3abYLkdk0xS02OKgFBBoC8k8QAvD-5FBwE&d=DwIGaQ&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=K-6G4PgDF8DqhiakFxI1pnea8lR2C9xQHqWnrYHUefk&s=imWJ8sVczAToQjB1FZDf_-zlB9uXzpNibMHeYlXCXqs&e= Stericycle is a compliance company that specializes in collecting and disposing regulated substances, such as medical waste and sharps, pharmaceuticals, hazardous waste, and providing services for recalled and expired goods. It also provides related education and training services, and patient communication services. Wikipedia Stock price: SRCL (NASDAQ) $63.65 +0.11 (+0.17%) Jan 29, 10:57 AM EST - Disclaimer Headquarters: Lake Forest, IL CEO: Cindy J. Miller (May 2, 2019?) Number of employees: 23,200 (2017) Founded: 1989 Subsidiaries: Shred-it, Stericycle Environmental Solutions, MORE -----Original Message----- From: Eileen Akemi Allison via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, January 29, 2020 4:25 AM To: Histonet > Cc: MirnaIbarra at umcelpaso.org Subject: [Histonet] Tissue disposal Good morning Histopeeps! After reviewing our Policy Procedure Manual, our manager is questioning the bags we are using for disposing our tissues after diagnosis is complete. We are separating the tissue from the formalin by straining the excess formalin and neutralizing it. We then put the tissue into ?Large Biohazard Bags? and the EVS staff picks it up and disposes it. My manager is wondering if the ?Biohazard Bags" are appropriate. Should the bags state ?Tissue? on the them? Are there any special disposal bags available? While working for a GI Lab in Monterey, CA we put all our bottle and tissue in "Biohazard Bags" and a Hazardous Waste company picked up the waste products from our endoscopy department along with ours. Any comments and suggestions would be greatly appreciated. Thank you in advance, Akemi Allison, BS, HT/HTL _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=zGJiNS11VbY0EhlUMdsr-w&r=JnmrOZ2W3u0Vym5qmaXBgNOijhynn3Nj4f0oLu1UdDo&m=tnwnbsEOJHiPAoYQtnkWynkJVqbIH7NbA32Sp1Btsns&s=_lD3rkVbKauOy3yifxm4MAk5WB-QrqSriKnDM_KV6ik&e= This email message and any files transmitted are sent with confidentiality in mind and contain privileged or copyright information. You must not present this message to another party without gaining permission from the sender. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. Any views expressed in this message are those of the sender, except where the sender specifically states them to be the views of Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. If you have received this message in error, please notify the sender immediately, and delete this email from your system. We do not guarantee that this material is free from viruses or any other defects although due care has been taken to minimize the risk. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=K-6G4PgDF8DqhiakFxI1pnea8lR2C9xQHqWnrYHUefk&s=5dp9S1KzWx99IwLcD8abFmJqY2IQ1sMMbLYR9RZPzS4&e= From lsorrells at revivicor.com Wed Jan 29 14:34:55 2020 From: lsorrells at revivicor.com (Lori Sorrells) Date: Wed, 29 Jan 2020 20:34:55 +0000 Subject: [Histonet] IHC on Pig Tissues Message-ID: Good afternoon- Has anyone run into problems when trying to perform IHC using rabbit mono or polyclonal antibodies on pig tissues? I typically do not have any issues with nonspecific staining or high background when using mouse monoclonal antibodies. I have used a variety of blockers including various sera in the diluents and blocking reagents. All samples are FFPE. I routinely use the DAKO EnVision+ Dual HRP system. Thanks in advance! Lori Sorrells Research Associate II Revivicor, Inc. 1700 Kraft Drive, Suite 2400 Blacksburg, VA 24060 From liz at premierlab.com Wed Jan 29 14:59:22 2020 From: liz at premierlab.com (Liz Chlipala) Date: Wed, 29 Jan 2020 20:59:22 +0000 Subject: [Histonet] IHC on Pig Tissues In-Reply-To: References: Message-ID: Lori You can't use a dual label detection system. We have found most mouse polymers will cross react with porcine tissue. We know that the dako one does. You need to use the rabbit envision for rabbit monoclonal antibodies or with mouse monoclonals you need to use a rabbit anti-mouse and then a rabbit envision detection system. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Lori Sorrells via Histonet Sent: Wednesday, January 29, 2020 1:35 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC on Pig Tissues Good afternoon- Has anyone run into problems when trying to perform IHC using rabbit mono or polyclonal antibodies on pig tissues? I typically do not have any issues with nonspecific staining or high background when using mouse monoclonal antibodies. I have used a variety of blockers including various sera in the diluents and blocking reagents. All samples are FFPE. I routinely use the DAKO EnVision+ Dual HRP system. Thanks in advance! Lori Sorrells Research Associate II Revivicor, Inc. 1700 Kraft Drive, Suite 2400 Blacksburg, VA 24060 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From tpodawiltz at yahoo.com Wed Jan 29 18:48:37 2020 From: tpodawiltz at yahoo.com (Thomas Podawiltz) Date: Thu, 30 Jan 2020 00:48:37 +0000 (UTC) Subject: [Histonet] FW: Tissue disposal In-Reply-To: References: Message-ID: <2130485710.1255860.1580345317435@mail.yahoo.com> After decanting the formalin off our tissue is bagged in biohazard bags that are in a biohazard box. The box is labeled pathology waste for incineration only.? On Wednesday, January 29, 2020, 02:52:17 PM EST, Rathborne, Toni via Histonet wrote: Stericycle will also take your blocks and slides when you're ready to discard them. Toni ------------------------------------------------------------------------------------ NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. -----Original Message----- From: Carol G Fields via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, January 29, 2020 2:05 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] FW: Tissue disposal *** This is an External Email *** FYI?_____________________________________________ From: Carol G Fields Sent: Wednesday, January 29, 2020 8:32 AM To: 'Eileen Akemi Allison' Subject: RE: [Histonet] Tissue disposal Hi, Stericycle takes care of all your waste, including decanting and disposal.? They are all over the US..Health & Safety alone plus employee time will justify you using these people.? No one should still be doing this? I?ve used this company at several hospitals. They are great. Good luck, Carole MLKCH Los Angeles, CA https://urldefense.proofpoint.com/v2/url?u=https-3A__www.stericycle.com_landing-2Dpages_minimal-2Dglobal-2Dwrapper_regulated-2Dmedical-2Dwaste-2Ddisposal-3Futm-5Fcampaign-3DSEM-5FOngoing-5FTopCoreExact-26utm-5Fsource-3DGoogle-26utm-5Fmedium-3Dcpc-26utm-5Fcontent-3DB-5FTop-5FCore-5FG-5FSEM-5FTxt-5FExact-26utm-5Fterm-3Dstericycle-26gclid-3DCjwKCAiA98TxBRBtEiwAVRLqu8nk2kjv5k4J-2Dd-5Fzb9menIfZRowLKKJY4Df3abYLkdk0xS02OKgFBBoC8k8QAvD-5FBwE&d=DwIGaQ&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=K-6G4PgDF8DqhiakFxI1pnea8lR2C9xQHqWnrYHUefk&s=imWJ8sVczAToQjB1FZDf_-zlB9uXzpNibMHeYlXCXqs&e= Stericycle is a compliance company that specializes in collecting and disposing regulated substances, such as medical waste and sharps, pharmaceuticals, hazardous waste, and providing services for recalled and expired goods. It also provides related education and training services, and patient communication services. Wikipedia Stock price: SRCL (NASDAQ) $63.65 +0.11 (+0.17%) Jan 29, 10:57 AM EST - Disclaimer Headquarters: Lake Forest, IL CEO: Cindy J. Miller (May 2, 2019?) Number of employees: 23,200 (2017) Founded: 1989 Subsidiaries: Shred-it, Stericycle Environmental Solutions, MORE -----Original Message----- From: Eileen Akemi Allison via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, January 29, 2020 4:25 AM To: Histonet > Cc: MirnaIbarra at umcelpaso.org Subject: [Histonet] Tissue disposal Good morning Histopeeps! After reviewing our Policy Procedure Manual, our manager is questioning the bags we are using for disposing our tissues after diagnosis is complete.? We are separating the tissue from the formalin by straining the excess formalin and neutralizing it.? We then put the tissue into ?Large Biohazard Bags? and the EVS staff picks it up and disposes it.? My manager is wondering if the ?Biohazard Bags" are appropriate.? Should the bags state ?Tissue? on the them?? Are there any special disposal bags available? While working for a GI Lab in Monterey, CA we put all our bottle and tissue in "Biohazard Bags" and a Hazardous Waste company picked up the waste products from our endoscopy department along with ours.? Any comments and suggestions would be greatly appreciated. Thank you in advance, Akemi Allison, BS, HT/HTL _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=zGJiNS11VbY0EhlUMdsr-w&r=JnmrOZ2W3u0Vym5qmaXBgNOijhynn3Nj4f0oLu1UdDo&m=tnwnbsEOJHiPAoYQtnkWynkJVqbIH7NbA32Sp1Btsns&s=_lD3rkVbKauOy3yifxm4MAk5WB-QrqSriKnDM_KV6ik&e= This email message and any files transmitted are sent with confidentiality in mind and contain privileged or copyright information. You must not present this message to another party without gaining permission from the sender. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. Any views expressed in this message are those of the sender, except where the sender specifically states them to be the views of Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. If you have received this message in error, please notify the sender immediately, and delete this email from your system. We do not guarantee that this material is free from viruses or any other defects although due care has been taken to minimize the risk. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=K-6G4PgDF8DqhiakFxI1pnea8lR2C9xQHqWnrYHUefk&s=5dp9S1KzWx99IwLcD8abFmJqY2IQ1sMMbLYR9RZPzS4&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Thu Jan 30 09:51:58 2020 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 30 Jan 2020 10:51:58 -0500 Subject: [Histonet] RELIA Histology Current Opportunities. Message-ID: <01cf01d5d785$34aaab00$9e000100$@earthlink.net> Hi Histonetters! ICYMI: Here is a link to my current Histology Careers Newsletter: https://reliasolutionspambarker.wordpress.com/2020/01/22/relia-histology-car eers-bulletin/ And Here is a link to my current blog post on Fixation on Histology - Coping with the Shortage of histotechs - Strategies for Managers and Techs. http://www.mmsend33.com/link.cfm?r=ctFwhDO4CZXdL1j7LvhQ2w~~&pe=kZ0wiL1XoQUMF cM2F6DbgaFIoGeczciv2VAX4rf3swGwI9JuzcNIICeyLY_xDzdoT8HTaHu1FHdiuQf0AnvKtA~~ &t=yXDbukAKRrBvgwEDXE6DMQ~~> here is a list of my current openings: Leadership Opportunities: Midwest U.S. Field Applications Specialist - IHC HT/HTL Panama City, FL Topeka, KS Greenville, SC Beaufort, SC Greensboro, NC Modesto, CA Kingsport, TN Austin, TX Milwaukee, WI If you or any of your friends would like to chat about a custom job search and how I can help or more information about any of the positions listed please contact me. I would be more than happy to assist you. You can reach me ASAP on my cell at 407-353-5070 or toll free at 866-607-3542 or relia1 at earthlink.net Remember it never hurts to look. Hope to hear from you soon. Thanks-Pam Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From chelsey at ka-recruiting.com Thu Jan 30 10:19:53 2020 From: chelsey at ka-recruiting.com (Chelsey Opalenik) Date: Thu, 30 Jan 2020 11:19:53 -0500 Subject: [Histonet] Histology Supervisor Needed: Florida Message-ID: Good Morning! Looking for a new histology supervisory position in Florida? A nationally ranked cancer hospital is looking for an experienced histology supervisor to join their team in Central/Western FL. - ASCP HTL - Histology Supervisory License Florida - Minimum of five years relevant Histology laboratory operations experience at the laboratory manager and/or supervisory level. - Minimum of five years experience at a histotechnologist level inclusive of IHC techniques, optimization, and validation prior to management/supervisory experience. *Preferred:* Knowledge or experience with Digital Pathology and Informatics. Proficient with Microsoft Office applications. For more information please send your resume to Chelsey at ka-recruiting.com! Thank you and have a great day! -- Chelsey Opalenik *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2605 From akemiat3377 at gmail.com Fri Jan 31 06:35:18 2020 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Fri, 31 Jan 2020 05:35:18 -0700 Subject: [Histonet] Histotech openings in El Paso, TX Message-ID: Happy Friday Histopeeps! Any Histotechs want to move to El Paso, TX? El Paso has a lot to offer! We have one current opening and another coming up in the next month. We are losing one of our best tech?s to FL. Her husband is a pathologist who is going to do his GI fellowship in Miami. Our lab is getting a Leica Upgrade starting in March! A Ceribro system, 2 new Bonds, a new H&E Stainer with Cover Slipper, and Cassette and Slide Printer. Email me and I will provide all information. Akemi Allison, BS, HT/HTL Histology Supervisor UMC El Paso, TX Any Histotech's want to move to El Paso? 2 openings coming up in my lab! Any Histotech's want to move to El Paso? 2 openings coming up in my lab! Any Histotech's want to move to El Paso? 2 openings coming up in my lab!