From mcruz84pr at gmail.com Mon Feb 3 19:47:36 2020 From: mcruz84pr at gmail.com (Maria Cruz) Date: Mon, 3 Feb 2020 20:47:36 -0500 Subject: [Histonet] Desperately seeking a certain IHC expert Message-ID: Hey everybody - This is an unusual request or at least I think it is. I?m hoping that someone can help me find and connect with a guy that I heard speak at the national histo-meeting. I?ve also seen his name as a presenter at several state meetings to. Hes involved with the national society and seems to always give presentations on IHC topics. I?m pretty sure that He works for one of the major IHC companies. The lab I work in has major IHC issues and he always seems to be able to answer everyone?s questions. Thanks in advance. Maria From liz at premierlab.com Mon Feb 3 20:04:56 2020 From: liz at premierlab.com (Liz Chlipala) Date: Tue, 4 Feb 2020 02:04:56 +0000 Subject: [Histonet] Desperately seeking a certain IHC expert In-Reply-To: References: Message-ID: Joe Myers biocate Sent from my Windows 10 device From: Maria Cruz via Histonet Sent: Monday, February 3, 2020 7:03 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Desperately seeking a certain IHC expert Hey everybody - This is an unusual request or at least I think it is. I?m hoping that someone can help me find and connect with a guy that I heard speak at the national histo-meeting. I?ve also seen his name as a presenter at several state meetings to. Hes involved with the national society and seems to always give presentations on IHC topics. I?m pretty sure that He works for one of the major IHC companies. The lab I work in has major IHC issues and he always seems to be able to answer everyone?s questions. Thanks in advance. Maria _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From akemiat3377 at gmail.com Tue Feb 4 06:42:06 2020 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Tue, 4 Feb 2020 05:42:06 -0700 Subject: [Histonet] HistoQIP/PIP Message-ID: <7F4D71BC-7020-48C8-8271-D342363BF08B@gmail.com> Hello Histopeeps: Can someone tell me what year HistoQip start with NSH/CAP and PIP with CAP. Thank you! Akemi Allison, BS, HT/HTL From 11z at comcast.net Tue Feb 4 12:39:33 2020 From: 11z at comcast.net (11z at comcast.net) Date: Tue, 4 Feb 2020 10:39:33 -0800 Subject: [Histonet] Histonet Digest, Vol 195, Issue 1 In-Reply-To: References: Message-ID: <004501d5db8a$71bf9b50$553ed1f0$@comcast.net> Hi, I am looking for a decent Gram +/- control tissue. Would be happy to exchange another type of control in exchange for a good block of tissue for this. I have great PAS or Warthan Starry or ? others too. Can someone help with this? Thanks. LeRoy Brown HT(ASCP) HTL 11z at comcast.net is my email Thanks -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, February 4, 2020 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 195, Issue 1 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From amurvosh at advancederm.net Tue Feb 4 14:03:14 2020 From: amurvosh at advancederm.net (Anne Murvosh) Date: Tue, 4 Feb 2020 20:03:14 +0000 Subject: [Histonet] Formaldehyde test kit question Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7CAC740@Exchange.Advancederm.net> We switched formalin neutralizing solution and I bought the Quantofix formaldehyde test kit to make sure it's working, however it does not mention what color is acceptable for neutralization. The options are 0-10-20- up to 200 MG/L. should it be "0" for acceptable dumping? Also the instructions say to add 10 drops of a sodium hydroxide solution to the solution being tested, wouldn't that change the Ph and make the results not as accurate. This kit is expensive if regular pH strips are better, let me know that too. Thanks for your help. Anne From cfields at mlkch.org Tue Feb 4 14:12:26 2020 From: cfields at mlkch.org (Carol G Fields) Date: Tue, 4 Feb 2020 20:12:26 +0000 Subject: [Histonet] Formaldehyde test kit question In-Reply-To: <22BDD9AABC13E24E95D1CF064B75C4B7CAC740@Exchange.Advancederm.net> References: <22BDD9AABC13E24E95D1CF064B75C4B7CAC740@Exchange.Advancederm.net> Message-ID: Hi Ann, The only certified formalin neutralizer that I know of is from Scigen. They also sell the test kit. Ask your company for their certification.. that the product actually works. Good luck, Carole Fields MLKCH Los Angeles, CA Scigen Scientific Inc. Manufacturer in Carson, California Address: 333 E Gardena Blvd, Gardena, CA 90248 Hours: Open ? Closes 4:30PM Phone: (310) 324-6576 -----Original Message----- From: Anne Murvosh via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, February 04, 2020 12:03 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Formaldehyde test kit question We switched formalin neutralizing solution and I bought the Quantofix formaldehyde test kit to make sure it's working, however it does not mention what color is acceptable for neutralization. The options are 0-10-20- up to 200 MG/L. should it be "0" for acceptable dumping? Also the instructions say to add 10 drops of a sodium hydroxide solution to the solution being tested, wouldn't that change the Ph and make the results not as accurate. This kit is expensive if regular pH strips are better, let me know that too. Thanks for your help. Anne _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=zGJiNS11VbY0EhlUMdsr-w&r=JnmrOZ2W3u0Vym5qmaXBgNOijhynn3Nj4f0oLu1UdDo&m=uW_WlErqCVeSOF3fe4wpGhdJbglOmO52fHzha4s4NCk&s=qFTKM-0jq7PPaa6zBTGeREOBs-iVMhQ2E7NM5ShPxbM&e= This email message and any files transmitted are sent with confidentiality in mind and contain privileged or copyright information. You must not present this message to another party without gaining permission from the sender. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. Any views expressed in this message are those of the sender, except where the sender specifically states them to be the views of Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. If you have received this message in error, please notify the sender immediately, and delete this email from your system. We do not guarantee that this material is free from viruses or any other defects although due care has been taken to minimize the risk. From jackie.hardamon at mldpathology.com Tue Feb 4 21:35:04 2020 From: jackie.hardamon at mldpathology.com (Jackie Hardamon) Date: Tue, 4 Feb 2020 21:35:04 -0600 Subject: [Histonet] Fwd: Available Histology job: Houston, Texas References: <61E7FBCE-5DD3-46BE-AB18-E2DB702D8D37@mldpathology.com> Message-ID: Hi, I?ve subscribed to the forum. Please reconsider my post for job listing. Jaclyn Hardamon, HT(ASCP)cm QIHC Client Labs Histology Supervisor jackie.hardamon at mldpathology.com MLD Pathology 1140 Business Center Drive, Suite 370 Houston, Texas 77043 M: (832) 657-4356 P: (713) 271-6881 F: (713) 271-6885 www.mldpathology.com Begin forwarded message: > From: Jackie Hardamon > Date: February 4, 2020 at 8:56:39 PM CST > To: histonet at lists.utsouthwestern.edu > Subject: Available Histology job: Houston, Texas > > ?Hi, > > I?d like to post my company?s open positions on your forum. We are seeking two Histology Techs to work at remote lab locations throughout Houston. > > https://docs.google.com/document/d/1RCNlRxY9knXseQB9i8mNCtaII5npNqwTRSEEByHPUcM > > Applicants may contact me at the info provided below. > > Many thanks, > > Jaclyn Hardamon, HT(ASCP)cm QIHC > Client Labs Histology Supervisor > jackie.hardamon at mldpathology.com > > MLD Pathology > 1140 Business Center Drive, Suite 370 > Houston, Texas 77043 > M: (832) 657-4356 > P: (713) 271-6881 > F: (713) 271-6885 > www.mldpathology.com > From Kelly.Pairan at nationwidechildrens.org Wed Feb 5 08:40:47 2020 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Wed, 5 Feb 2020 14:40:47 +0000 Subject: [Histonet] Lin28 Message-ID: Good Morning, We are currently looking at our Lin28 staining and were wondering what other people are using in their labs. Thanks, Kelly From edonato at fredhutch.org Wed Feb 5 15:56:58 2020 From: edonato at fredhutch.org (Donato, Elizabeth M) Date: Wed, 5 Feb 2020 21:56:58 +0000 Subject: [Histonet] Pathology Devices TMArrayer punches Message-ID: Hi Everyone, I'm trying to get a hold of some punch sets (0.6,1.0,2.0mm) from Pathology Devices and they are not responding to phone calls or emails. There is at least one other histonet post from an individual in a similar situation. I think that they may be out of business. My question to you all is whether there is anyone out there with a Pathology Devices TMArrayer that you're not using? If so, do you have any new or slightly used punches that you might be willing to sell to us? I'm also interested in getting a hold of punch adaptors that would allow us to use our Beecher/Estigen punches on the TMArrayer. Any help would be much appreciated! Thank you, Best, Liz Liz Donato MT(ASCP) Manager | Porter and Specialized Pathology Laboratories ********************************************************************************************* 206.667.4501 p | 206.667.5815 f | edonato at FredHutch.org [http://www.fredhutch.org/content/dam/public/email-signatures/3/fred_hutch_logo.png] Fred Hutchinson Cancer Research Center 1100 Fairview Ave. N., Mail Stop C1-015 Seattle, WA 98109 fredhutch.org [http://www.fredhutch.org/content/dam/public/email-signatures/3/socialmedia_footer_1.png][http://www.fredhutch.org/content/dam/public/email-signatures/3/socialmedia_footer_2.png][http://www.fredhutch.org/content/dam/public/email-signatures/3/socialmedia_footer_3.png][http://www.fredhutch.org/content/dam/public/email-signatures/3/socialmedia_footer_4.png][http://www.fredhutch.org/content/dam/public/email-signatures/3/socialmedia_footer_5.png][http://www.fredhutch.org/content/dam/public/email-signatures/3/socialmedia_footer_6.png] From jramos at biocare.net Wed Feb 5 19:27:25 2020 From: jramos at biocare.net (Jason Ramos) Date: Thu, 6 Feb 2020 01:27:25 +0000 Subject: [Histonet] Temporary HT Position Message-ID: Hello Histonetters- Biocare Medical is searching for a temporary Histotechnologist to meet our increased slide demand. Additional information is below. If anyone is interested, please contact me directly (jramos at biocare.net). Hours: 4 hours/shift, 1 - 2 shifts per week (preferably during daytime hours) Tasks: Sectioning FFPE blocks, mainly tonsils, with good quality Requirements: Experienced HT, who can cut slides with speed and quality. Attention to detail is critical. Thank You, Jason Jason A. Ramos, Ph.D. | Vice President - Reagent Research and Development Phone: 925.603.8098 | Cell: 925.768.2792 | www.biocare.net Biocare Medical, LLC 60 Berry Drive, Pacheco CA 94553 This electronic transmission (and any attached document) contains information from Biocare Medical, LLC. They are intended only for named recipients above, and contain information that may be confidential or privileged. If you are not an intended recipient, you must not read, copy, use or disclose this communication. Please also notify the sender by replying to this message, and then delete all copies of it from your system. From arunjyothisp at gmail.com Thu Feb 6 09:18:00 2020 From: arunjyothisp at gmail.com (Arun Jyothi S.P) Date: Thu, 6 Feb 2020 20:48:00 +0530 Subject: [Histonet] How to Reduce Tissue Autofluorescence Message-ID: Dear All, Kindly share your working protocol using ?home brew? reagents to reduce tissue auto-fluorescence. Thank you Arun Jyothi S.P. Research Assistant Cancer Research RGCB Trivandrum From relia1 at earthlink.net Thu Feb 6 10:37:58 2020 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 6 Feb 2020 11:37:58 -0500 Subject: [Histonet] RELIA Hot Job Alert - Lead Tech - Histology - Days - SE Michigan - 10K Sign On bonus! Message-ID: <010401d5dd0b$cafd8a10$60f89e30$@earthlink.net> Hi Histopeeps! I hope this has been a great week. I wanted to let you know about a new position I am working on: Here is the posting: Lead Histology Tech - Detroit, MI 10,000 Sign On Bonus! RELIA Solutions has been engaged by a busy lab located in Detroit, MI. They are in need of a histotechnologist for their Day shift. ASCP HT/HTL certification and 2 years of histology experience is required. Our client offers a competitive salary, nice benefits and a $10,000.00sign on bonus. This is a full time permanent Day shift position!! For more information please contact Pam Barker at relia1 at earthlink.net or toll free at 866-607-3542. RELIA Solutions is the nation's ONLY recruiting firm specializing in the nationwide permanent placement of histology professionals. To sign up for our free histology careers newsletter please send an e-mail to relia1 at earthlink.net and include subscribe in the subject line. Keywords: histology, histologist, histotechnician, histotechnologist, MI #ilovemyhistopeeps #jobs4myhistopeeps In case you missed it here is a link to the latest issue of RELIA's Histology Careers Newsletter: https://reliasolutionspambarker.wordpress.com/2020/02/04/relia-solutions-his tology-careers-newsletter/ Thanks-Pam Right Time, Right Place, Right Move with RELIA! #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Carole.Johnson at uchealth.org Thu Feb 6 13:56:06 2020 From: Carole.Johnson at uchealth.org (Johnson, Carole) Date: Thu, 6 Feb 2020 19:56:06 +0000 Subject: [Histonet] Changing to reagent alcohol Message-ID: We have been using ethanol in our lab and would like to change to using reagent alcohol for the obvious reasons of cost and regulatory headaches. I have been tasked with creating a validation/verification process. Does anyone have any suggestions? Or better yet, information proving that we can make the change without going through an extensive project? Thanks! Carole L Johnson, HT(ASCP)cm, QIHC(ASCP)cm Histotechnologist UCH/Memorial Central Hospital Histology Laboratory 1400 Boulder Street Colorado Springs, CO O 719.365.5204 carole.johnson at uchealth.org CONFIDENTIALITY NOTICE: This message and any attachments may contain privileged and confidential peer review, risk management, and/or quality management information pursuant to the Colorado Professional Review Act, C.R.S. 12-36.5-101, et seq., the Colorado Hospital Licensing law, C.R.S. 25-3-109, the Quality Management Programs law, C.R.S. 25-35-904, and other corresponding provisions of federal and state law. Please maintain the strict confidentiality of this information. If you are not the intended recipient, you are notified that any disclosure, copying, distribution, electronic storage or use of this communication is prohibited. If you received this communication in error, please notify us immediately by e-mail, attaching the original message, and delete the original message from your computer and any network to which your computer is connected. From patpxs at gmail.com Thu Feb 6 16:51:00 2020 From: patpxs at gmail.com (Patpxs) Date: Thu, 6 Feb 2020 14:51:00 -0800 Subject: [Histonet] Changing to reagent alcohol In-Reply-To: References: Message-ID: <2D9C3EC8-6E6B-4C6A-9C30-7714FAD33731@gmail.com> Hi Carole, Sorry but you need to validate the change completely. Reagent alcohols are blends that sometimes don?t even contain ethanol. You need to demonstrate that it doesn?t affect routine H&Es, special stains, and IHC. Plus you have to test the major tissue types you routinely work on. Others may disagree with me, but if you get an inspector who focuses on the minutia, you can get cited. Paula Sent from my iPhone > On Feb 6, 2020, at 12:13 PM, Johnson, Carole via Histonet wrote: > > ?We have been using ethanol in our lab and would like to change to using reagent alcohol for the obvious reasons of cost and regulatory headaches. I have been tasked with creating a validation/verification process. Does anyone have any suggestions? Or better yet, information proving that we can make the change without going through an extensive project? Thanks! > > Carole L Johnson, HT(ASCP)cm, QIHC(ASCP)cm > Histotechnologist > UCH/Memorial Central Hospital > Histology Laboratory > 1400 Boulder Street > Colorado Springs, CO > O 719.365.5204 > carole.johnson at uchealth.org > > CONFIDENTIALITY NOTICE: This message and any attachments may contain privileged and confidential peer review, risk management, and/or quality management information pursuant to the Colorado Professional Review Act, C.R.S. 12-36.5-101, et seq., the Colorado Hospital Licensing law, C.R.S. 25-3-109, the Quality Management Programs law, C.R.S. 25-35-904, and other corresponding provisions of federal and state law. Please maintain the strict confidentiality of this information. If you are not the intended recipient, you are notified that any disclosure, copying, distribution, electronic storage or use of this communication is prohibited. If you received this communication in error, please notify us immediately by e-mail, attaching the original message, and delete the original message from your computer and any network to which your computer is connected. > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nicolefoote10 at gmail.com Thu Feb 6 19:31:09 2020 From: nicolefoote10 at gmail.com (nicole foote) Date: Thu, 6 Feb 2020 20:31:09 -0500 Subject: [Histonet] Kappa and lambda IHC Message-ID: <25A6EA32-9C3C-47C8-87B2-98B8480F5EF1@gmail.com> Hi all, I was wondering if anyone can provide information or tips on the protocols for running kappa and lambda IHC on Ventana?s benchmark ULTRA. I have reagents from Biocare that have been prepped for use with the benchmark as my pathologists were not satisfied with the reagents from ventana in the past. Thank you in advance, Nikki From jess.hall at wvumedicine.org Fri Feb 7 08:35:33 2020 From: jess.hall at wvumedicine.org (Hall, Jessica) Date: Fri, 7 Feb 2020 14:35:33 +0000 Subject: [Histonet] WVU Medicine Histotechnologist Message-ID: <437FE806DAB7B441A526B805E086396E42119A01@NT-EX2.wvuhs.com> Good Morning, We recently opened another new Histotechnologist position with WVU Medicine! This position is eligible for a generous sign-on bonus, full comprehensive benefit package, and a competitive salary range. I invite you to contact me with any questions about this exciting opportunity to join the region's largest health system! To submit your application for consideration, click here: https://re12.ultipro.com/WES1019WVUH/jobboard/NewCandidateExt.aspx?__JobID=48450 Here to watch video about our Health System: / Jessica Hall, CIR, SHRM-CP, AASPR, STA Supervisor, Talent Acquisition Human Resources WVU Medicine Office: 304-598-6689 Cell: 304-709-4783 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From rsrichmond at gmail.com Fri Feb 7 12:33:42 2020 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 7 Feb 2020 13:33:42 -0500 Subject: [Histonet] Changing to reagent alcohol In-Reply-To: References: Message-ID: Carole L Johnson, HT(ASCP)cm, QIHC(ASCP)cm at UCH/Memorial Central Hospital in Colorado Springs asks: >>We have been using ethanol in our lab and would like to change to using reagent alcohol for the obvious reasons of cost and regulatory headaches. I have been tasked with creating a validation/verification process. Does anyone have any suggestions? Or better yet, information proving that we can make the change without going through an extensive project?<< What's commonly called reagent alcohol in histology is 90% ethanol, 5% methanol, and 5% isopropanol. BATF calls this "SD3 modified" (regulation SD3 is denatured only with methanol). You sometimes see a different denaturant, methyl isobutyl ketone (MIBK), but it smells so bad most labs don't use it. Alcohol denatured with acetone is also available, and is NOT suitable for histologic use (it removes the eosin in the dehydration sequence). The proposal is to replace absolute ethanol, in order to avoid the nuisance of complying with BATF regulations on potentially drinkable alcohol. I've never seen a practical problem with this changeover. I have no idea what the regulatory issues might be. Bob Richmond Samurai Pathologist Maryville TN From walker_pam at hotmail.com Sat Feb 8 11:08:52 2020 From: walker_pam at hotmail.com (Pamela Walker) Date: Sat, 8 Feb 2020 17:08:52 +0000 Subject: [Histonet] Fw: Job Offer in El Paso In-Reply-To: References: Message-ID: ________________________________ From: Pamela Walker Sent: Saturday, February 8, 2020 10:51 AM To: histonet at lists.utsouthwestern.edu Cc: Pamela Walker Subject: Job Offer in El Paso Greetings Ms. Eileen A. Allison... I saw your recent post concerning an opening in your lab. I am sorry your losing a great tech but are open to recieve even greater techs. I am so excited your lab is getting upgrades that always a plus for good productivity in the lab. My name is Pam Walker. I am currently working in Austin at a high-volume lab for over a year. I mainly cut surgicals, derm, biopsies, prostates, specials and recuts. I do embed but as needed. I would definitely be interested in the position and I am will to relocate if hired. I look forward to all the information concerning this great opportunity. Pam Walker, HT From carl.hobbs at kcl.ac.uk Sat Feb 8 12:56:09 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sat, 8 Feb 2020 18:56:09 +0000 Subject: [Histonet] How to Reduce Tissue Autofluorescence Message-ID: Hi Do you refer to FIF? Or...autofluorescence? Different...as you prob know so, apologies in advance. The Wright Cell imaging Facility ( Toronto western research Inst) pdf: very informative FIF: I have tried Glycine, Ammonium chloride and Na-borohyd. I got best results with Glycine However....none are great. Multi spectral imaging is the best way forwards but....expensive. Sure, in Lambda mode using Zeiss Zen on a confocal gives good results ( tho I have forgotten how to do it, sigh) After setting up you hit the fluorescence you don't want...hit the one you want....if the wavelengths are different, what you don't want you eliminate, electronically. Vectorlabs sell an excellent kit for FIF elimination...sure over-expensive, given the ingredients. We found that dilution of their working reagent by x2- x4 gave best results As good as multispectral imaging, imho. Autofl of lipofuscin is supressed using Sudan BlackB. Good luck Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From jkiernan at uwo.ca Sat Feb 8 23:55:27 2020 From: jkiernan at uwo.ca (John Kiernan) Date: Sun, 9 Feb 2020 05:55:27 +0000 Subject: [Histonet] How to Reduce Tissue Autofluorescence In-Reply-To: References: Message-ID: There's a very brief article (downloadable PDF) from 2002 about suppressing autofluorescence, with a few references, at https://www.researchgate.net/publication/10971457_Suppressing_autofluorescence [https://i1.rgstatic.net/publication/10971457_Suppressing_autofluorescence/links/00463520275ec6a5f1000000/largepreview.png] (PDF) Suppressing autofluorescence - ResearchGate A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. www.researchgate.net This PDF file also has short peer-reviewed histotechnical tips on 5 other topics. Fun for all there. For something more recent on autofluorescence, try: Davis AS, Richter A, Becker S, Moyer JE, Sandouk A, Skinner J, Taubenberger JK (2014) Characterizing and diminishing autofluorescence in formalin-fixed paraffin-embedded human respiratory tissue. J. Histochem. Cytochem. 62: 405-423. They compared 9 procedures and favoured 3: sodium borohydride, Sudan black B and another dye called eriochrome black T. The last-named dye is CI 14645, Mordant black 11, a monoazo dye very briefly described on page 108 in Conn's 9th edn (1977) with the preferred name chromogen black ETOO; it's not in Conn's 10th edn (2002). Sodium borohydride reacts with aldehydes and probably reduces fixative-induced fluorescence of proteins and the native fluorescence of lipofuscins. The black dyes may work by absorbing more weakly emitted light. Sudan black B can stain lipofuscin black even in in paraffin sections. Using ?home brew? reagents is always the best way to go, because you can avoid buying simple products sold at high prices with fancy names. Avoid trying anyone's unexplained "working protocol" because annotated pieces of paper get passed along in labs and can induce well educated people to do things that are obviously wrong. It is necessary to know the reason for each step in a lab procedure. You identify as a research assistant, so you must have a boss. Probably your boss should be online along with you, asking histonetters for advice about reducing autofluorescence. That's quite enough from me, on 9 Feb 2020. John Kiernan (Anatomy, UWO, London, Canada) = = = ________________________________ From: Arun Jyothi S.P via Histonet Sent: 06 February 2020 10:18 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] How to Reduce Tissue Autofluorescence Dear All, Kindly share your working protocol using ?home brew? reagents to reduce tissue auto-fluorescence. Thank you Arun Jyothi S.P. Research Assistant Cancer Research RGCB Trivandrum _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From karim.ruknuddin at aku.edu Sun Feb 9 22:31:10 2020 From: karim.ruknuddin at aku.edu (Karim Ruknuddin) Date: Mon, 10 Feb 2020 04:31:10 +0000 Subject: [Histonet] Histonet Digest, Vol 195, Issue 6 In-Reply-To: References: Message-ID: <20deeef16c1247cd93e8700da6797982@EXMBXCAS1.aku.edu> Dear All, I am planning to introduce RNAscope in my pathology core facility, we are currently gathering information on both chromogen detection and florescent detection, but the company we are looking into for reagent ordering which is ACDbio has a wide range of channel 1 and channel 2 probes of human species, but limited channel 3 and channel 4 probes, they say you can even custom design your probes but they are expensive. Can anyone refer What detection system are you using or would recommend and why? Regards Karim Research Associate Aga Khan University Hospital Karachi -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Sunday, February 9, 2020 11:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 195, Issue 6 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Ckarim.ruknuddin%40aku.edu%7C9a6dffb091094523889608d7ad8a0353%7Ca5d4252a02f94e6096f09733baae4919%7C0%7C0%7C637168680646861537&sdata=A6arSdEEBvFJQQKBLOohJb5c3MwCsL5WiuP%2Bct4Ei%2F4%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: How to Reduce Tissue Autofluorescence (Hobbs, Carl) 2. Re: How to Reduce Tissue Autofluorescence (John Kiernan) ---------------------------------------------------------------------- Message: 1 Date: Sat, 8 Feb 2020 18:56:09 +0000 From: "Hobbs, Carl" To: histonet Subject: Re: [Histonet] How to Reduce Tissue Autofluorescence Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Do you refer to FIF? Or...autofluorescence? Different...as you prob know so, apologies in advance. The Wright Cell imaging Facility ( Toronto western research Inst) pdf: very informative FIF: I have tried Glycine, Ammonium chloride and Na-borohyd. I got best results with Glycine However....none are great. Multi spectral imaging is the best way forwards but....expensive. Sure, in Lambda mode using Zeiss Zen on a confocal gives good results ( tho I have forgotten how to do it, sigh) After setting up you hit the fluorescence you don't want...hit the one you want....if the wavelengths are different, what you don't want you eliminate, electronically. Vectorlabs sell an excellent kit for FIF elimination...sure over-expensive, given the ingredients. We found that dilution of their working reagent by x2- x4 gave best results As good as multispectral imaging, imho. Autofl of lipofuscin is supressed using Sudan BlackB. Good luck Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 ------------------------------ Message: 2 Date: Sun, 9 Feb 2020 05:55:27 +0000 From: John Kiernan To: "histonet at lists.utsouthwestern.edu" ,Arun Jyothi S.P Subject: Re: [Histonet] How to Reduce Tissue Autofluorescence Message-ID: Content-Type: text/plain; charset="Windows-1252" There's a very brief article (downloadable PDF) from 2002 about suppressing autofluorescence, with a few references, at https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.researchgate.net%2Fpublication%2F10971457_Suppressing_autofluorescence&data=02%7C01%7Ckarim.ruknuddin%40aku.edu%7C9a6dffb091094523889608d7ad8a0353%7Ca5d4252a02f94e6096f09733baae4919%7C0%7C0%7C637168680646861537&sdata=gT64wOQByicxQn6OF9%2Fnu4A8rylTvcWFfYul736i7kA%3D&reserved=0 [https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fi1.rgstatic.net%2Fpublication%2F10971457_Suppressing_autofluorescence%2Flinks%2F00463520275ec6a5f1000000%2Flargepreview.png&data=02%7C01%7Ckarim.ruknuddin%40aku.edu%7C9a6dffb091094523889608d7ad8a0353%7Ca5d4252a02f94e6096f09733baae4919%7C0%7C0%7C637168680646861537&sdata=0sxLU%2BhhEA75T3Wz5c5kSBDbmQPsICiOXjrw2vQNEvc%3D&reserved=0] (PDF) Suppressing autofluorescence - ResearchGate A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. https://eur03.safelinks.protection.outlook.com/?url=www.researchgate.net&data=02%7C01%7Ckarim.ruknuddin%40aku.edu%7C9a6dffb091094523889608d7ad8a0353%7Ca5d4252a02f94e6096f09733baae4919%7C0%7C0%7C637168680646871533&sdata=lg%2BYXmUV%2FyULhyGDWcQxLhuALJzt4e9hNFAKQS4ioEo%3D&reserved=0 This PDF file also has short peer-reviewed histotechnical tips on 5 other topics. Fun for all there. For something more recent on autofluorescence, try: Davis AS, Richter A, Becker S, Moyer JE, Sandouk A, Skinner J, Taubenberger JK (2014) Characterizing and diminishing autofluorescence in formalin-fixed paraffin-embedded human respiratory tissue. J. Histochem. Cytochem. 62: 405-423. They compared 9 procedures and favoured 3: sodium borohydride, Sudan black B and another dye called eriochrome black T. The last-named dye is CI 14645, Mordant black 11, a monoazo dye very briefly described on page 108 in Conn's 9th edn (1977) with the preferred name chromogen black ETOO; it's not in Conn's 10th edn (2002). Sodium borohydride reacts with aldehydes and probably reduces fixative-induced fluorescence of proteins and the native fluorescence of lipofuscins. The black dyes may work by absorbing more weakly emitted light. Sudan black B can stain lipofuscin black even in in paraffin sections. Using ?home brew? reagents is always the best way to go, because you can avoid buying simple products sold at high prices with fancy names. Avoid trying anyone's unexplained "working protocol" because annotated pieces of paper get passed along in labs and can induce well educated people to do things that are obviously wrong. It is necessary to know the reason for each step in a lab procedure. You identify as a research assistant, so you must have a boss. Probably your boss should be online along with you, asking histonetters for advice about reducing autofluorescence. That's quite enough from me, on 9 Feb 2020. John Kiernan (Anatomy, UWO, London, Canada) = = = ________________________________ From: Arun Jyothi S.P via Histonet Sent: 06 February 2020 10:18 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] How to Reduce Tissue Autofluorescence Dear All, Kindly share your working protocol using ?home brew? reagents to reduce tissue auto-fluorescence. Thank you Arun Jyothi S.P. Research Assistant Cancer Research RGCB Trivandrum _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Ckarim.ruknuddin%40aku.edu%7C9a6dffb091094523889608d7ad8a0353%7Ca5d4252a02f94e6096f09733baae4919%7C0%7C0%7C637168680646871533&sdata=3PgdcXfLesIRk1tnWIAIGa8SHAXFJhSuCUZI%2B8sx9XM%3D&reserved=0 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Ckarim.ruknuddin%40aku.edu%7C9a6dffb091094523889608d7ad8a0353%7Ca5d4252a02f94e6096f09733baae4919%7C0%7C0%7C637168680646871533&sdata=3PgdcXfLesIRk1tnWIAIGa8SHAXFJhSuCUZI%2B8sx9XM%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 195, Issue 6 **************************************** This message may contain confidential and/or privileged information. If you are not the intended recipient (or have received this message in error), please delete it and any attachments from your system, and notify the sender immediately. Any unauthorized use, dissemination, distribution, copy or disclosure of this communication is strictly prohibited. From madeathridge at pastnashville.com Mon Feb 10 10:20:01 2020 From: madeathridge at pastnashville.com (Maryann Deathridge) Date: Mon, 10 Feb 2020 16:20:01 +0000 Subject: [Histonet] (no subject) Message-ID: Hello All- We have a DRS 2000 slide stainer (new to us) in our laboratory. We are staining only routine H&E slides. I am not sure we have optimal programmed established. It seems to stain slower (even though) its double racks. Does anyone have a routine H&E protocol (we use clarifier and bluing) established that we could use? I'm thinking maybe we don't have the correct icon (exact, infinity) by the correct component. Any thoughts, ideas appreciated. If anyone has any experience with this unit please contact me or send me an email (madeathridge at pastnashville.com) From Branka.BruknerDabovic at nyulangone.org Mon Feb 10 15:58:04 2020 From: Branka.BruknerDabovic at nyulangone.org (Brukner Dabovic, Branka) Date: Mon, 10 Feb 2020 21:58:04 +0000 Subject: [Histonet] F480 antibody Message-ID: Could you please tell me what is your experience with F480 antibody from Cell Signaling: F4/80 (D2S9R) XP? Rabbit mAb #70076? Did you see any non-specific staining in IHC on paraffin sections? Thank you! Branka From criley at dpspa.com Tue Feb 11 09:33:12 2020 From: criley at dpspa.com (Charles Riley) Date: Tue, 11 Feb 2020 10:33:12 -0500 Subject: [Histonet] Warthin starry Message-ID: Is there any way to lighten and over stainer WS stain? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From tbraud at holyredeemer.com Tue Feb 11 14:05:03 2020 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 11 Feb 2020 20:05:03 +0000 Subject: [Histonet] overstained Warthin starry Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B74D15@HRHEX02-HOS.holyredeemer.local> Any silver stains can be lightened or removed by the old method for removing mercury pigment. This particular timing will remove it all so I would start the sequence of steps in 5 second increments, repeat or lengthen times as needed. 1. Hydrate to distilled water. 2.Place in Lugols iodine for 5 minutes. 3.Rinse in two changes of distilled water. 4.Place in 2% sodium thiosulfate for 1 minute. 5.Wash in running tap water and rinse in two changes of distilled water. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Message: 2 Date: Tue, 11 Feb 2020 10:33:12 -0500 From: Charles Riley To: Histo List Subject: [Histonet] Warthin starry Message-ID: Content-Type: text/plain; charset="UTF-8" Is there any way to lighten and over stainer WS stain? Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From relia1 at earthlink.net Wed Feb 12 09:36:01 2020 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 12 Feb 2020 10:36:01 -0500 Subject: [Histonet] RELIA HOT JOB ALERT and icymi a link to issue 3 of our newsletter. Message-ID: <00ce01d5e1ba$2225d8d0$66718a70$@earthlink.net> Hi Histopeeps, I have some great histology opportunities to tell you about. All of these are permanent full time positions and our clients offer excellent compensation, benefits, relocation and or sign on bonuses. SPOTLIGHT OPPORTUNITIES! **Sales Manager - Histology Ohio and Mid-east Region** **Group Lead ? SE Michigan full time days 10K Sign on Bonus!!** **I need Mohs Techs for several locations in North Carolina!!** Management: Histology Territory Sales Manager ? Mid East Region Histotechnology Program Director ? Orlando, FL Group Lead ? SE Michigan full time days 10K Sign on Bonus!! HT/HTL Opportunities: Modesto, CA Panama City, FL Bradenton, FL Ft. Myers, FL Kingsport, TN Topeka, KS Austin, TX Milwaukee, WI If you are interested in any of these positions please call or text me on my cell at 407-353-5070 or toll free at 866-607-3542 or e-mail me at relia1 at earthlink.net If you would like you can e-mail me your resume and a number where I can reach you at a time that is convenient for you. If you are interested in looking into new job opportunities in other areas that are not mentioned above please contact me as well. Not ALL of the positions I am working on are posted here. I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. ICYMI... Here is the link to the latest issue of RELIA's Histology Careers Newsletter: https://reliasolutionspambarker.wordpress.com/2020/02/04/relia-solutions-his tology-careers-newsletter/ Have a Wonderful Valentine?s Day!! Thanks-Pam 866-607-3542 (866-60RELIA) 407-353-5070-cell ? Right Time, Right Place, Right Move with RELIA! #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From akemiat3377 at gmail.com Thu Feb 13 06:42:05 2020 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Thu, 13 Feb 2020 05:42:05 -0700 Subject: [Histonet] histologists assisting with transplants Message-ID: <2F6FDD33-5C45-4CA9-8535-AB45848E467E@gmail.com> Good morning histoland! Just curious, this is definitely a 1st for me. Received a call this morning from my histotech who is on call this week that she was called in at 3:30 AM to assist one our pathologists with a liver transplant. In all my years working in this field, histologists have never had anything to do with transplants. Have any of you out there had anything to do with transplants? Akemi Allison, BS, HTL Histology Supervisor UMC El Paso, TX From john.garratt at ciqc.ca Thu Feb 13 09:49:08 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 13 Feb 2020 15:49:08 +0000 Subject: [Histonet] histologists assisting with transplants In-Reply-To: <2F6FDD33-5C45-4CA9-8535-AB45848E467E@gmail.com> References: <2F6FDD33-5C45-4CA9-8535-AB45848E467E@gmail.com> Message-ID: <40BxrUNsI1gxpNIiv9rGj15ZWy01ar1eAuY9r6xabNbshPcOod16KPXT2H7wKsxOIm6Q2O3LzC-z6EZSZToi5mEXe7yUAcLnQ_aZtnZoYjk=@ciqc.ca> Without knowing the specifics it could be simply to assist at frozen section to confirm the eligibility of the donor organ.Dig deeper. www.cpqa.ca ??????? Original Message ??????? On Thursday, February 13, 2020 4:42 AM, Eileen Akemi Allison via Histonet wrote: > Good morning histoland! > Just curious, this is definitely a 1st for me. Received a call this morning from my histotech who is on call this week that she was called in at 3:30 AM to assist one our pathologists with a liver transplant. In all my years working in this field, histologists have never had anything to do with transplants. Have any of you out there had anything to do with transplants? > > Akemi Allison, BS, HTL > Histology Supervisor > UMC El Paso, TX > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen at parrishmed.com Thu Feb 13 10:34:16 2020 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Thu, 13 Feb 2020 16:34:16 +0000 Subject: [Histonet] [EXTERNAL Sender] histologists assisting with transplants In-Reply-To: References: <2F6FDD33-5C45-4CA9-8535-AB45848E467E@gmail.com> Message-ID: -----Original Message----- From: Hannen, Valerie Sent: Thursday, February 13, 2020 10:50 AM To: 'Eileen Akemi Allison' Subject: RE: [EXTERNAL Sender] [Histonet] histologists assisting with transplants The only way that I have been involved with anything to do with any kind of transplant was to do a FS on the donor tissue, to ensure that it was a good transplantable organ. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Eileen Akemi Allison via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 13, 2020 7:42 AM To: Histonet Subject: [EXTERNAL Sender] [Histonet] histologists assisting with transplants This message came from an external source. Please do not click links or open attachments if unexpected or unusual. Begin Original Message: ---------------------------------------------------------------------- Good morning histoland! Just curious, this is definitely a 1st for me. Received a call this morning from my histotech who is on call this week that she was called in at 3:30 AM to assist one our pathologists with a liver transplant. In all my years working in this field, histologists have never had anything to do with transplants. Have any of you out there had anything to do with transplants? Akemi Allison, BS, HTL Histology Supervisor UMC El Paso, TX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PaPH0eDnAlaiSnq7Yv-U!ojZKih_v3GjCqE-m83bT6ADWtlPNJ4iLqZrKlTfh3RR3BcI2M-vI5hyWle4K4LiEDVspJQ$ From sandra.cheasty at wisc.edu Thu Feb 13 12:27:10 2020 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Thu, 13 Feb 2020 18:27:10 +0000 Subject: [Histonet] Autopsy Question Message-ID: Hello all, Actually it's a necropsy question for those of you who work in veterinary medicine. We perform necropsies on a very large variety of vertebrates that require removal of the spinal cord. (From hedgehogs to bovines.) What equipment are you using to remove spinal cords on different species? Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From renafail2 at gmail.com Thu Feb 13 17:32:14 2020 From: renafail2 at gmail.com (Rena Fail) Date: Thu, 13 Feb 2020 18:32:14 -0500 Subject: [Histonet] [EXTERNAL Sender] histologists assisting with transplants In-Reply-To: References: <2F6FDD33-5C45-4CA9-8535-AB45848E467E@gmail.com> Message-ID: when I worked, I did a lot of histology on both native and donor livers. I would not have used the term assisting with a liver transplant but on rare occasions, I had requests for a rapid Oil red O for fat on the donor liver In order for the pathologist to better determine the percentage of fat in the donor liver. Too much fat and it might not be considered for a transplant. Maybe it is just a matter of terminology. After all the pathologist and surgeon called her in at 3am and may have thanked her for her assistance. In 30 years I may have done 3 rapid ORO's on donor livers Rena Fail retired On Thu, Feb 13, 2020 at 11:44 AM Hannen, Valerie via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > > -----Original Message----- > From: Hannen, Valerie > Sent: Thursday, February 13, 2020 10:50 AM > To: 'Eileen Akemi Allison' > Subject: RE: [EXTERNAL Sender] [Histonet] histologists assisting with > transplants > > The only way that I have been involved with anything to do with any kind > of transplant was to do a FS on the donor tissue, to ensure that it was a > good transplantable organ. > > > > Valerie Hannen,MLT(ASCP),HTL,SU (FL) > Section Chief, Histology > Parrish Medical Center > 951 N. Washington Ave. > Titusville,Florida 32796 > T: (321)268-6333 ext. 7506 > F: (321) 268-6149 > valerie.hannen at parrishmed.com > www.parrishmed.com > > > > -----Original Message----- > From: Eileen Akemi Allison via Histonet [mailto: > histonet at lists.utsouthwestern.edu] > Sent: Thursday, February 13, 2020 7:42 AM > To: Histonet > Subject: [EXTERNAL Sender] [Histonet] histologists assisting with > transplants > > > This message came from an external source. Please do not click links or > open attachments if unexpected or unusual. > > Begin Original Message: > > ---------------------------------------------------------------------- > Good morning histoland! > Just curious, this is definitely a 1st for me. Received a call this > morning from my histotech who is on call this week that she was called in > at 3:30 AM to assist one our pathologists with a liver transplant. In all > my years working in this field, histologists have never had anything to do > with transplants. Have any of you out there had anything to do with > transplants? > > Akemi Allison, BS, HTL > Histology Supervisor > UMC El Paso, TX > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PaPH0eDnAlaiSnq7Yv-U!ojZKih_v3GjCqE-m83bT6ADWtlPNJ4iLqZrKlTfh3RR3BcI2M-vI5hyWle4K4LiEDVspJQ$ > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From raestask at grics.net Fri Feb 14 08:55:50 2020 From: raestask at grics.net (raestask) Date: Fri, 14 Feb 2020 08:55:50 -0600 Subject: [Histonet] Animal and Human Tissues Message-ID: <5E.E0.30881.8F4B64E5@smtp04.onyx.dfw.sync.lan> Is anyone processing both animal and human? tissues? If so my Lab Director would like to talk with you.Rae Ann Staskiewicz HT(ASCP)UnityPoint Methodist Peoria 309-672-4930Sent from my Verizon, Samsung Galaxy smartphone From mpence at grhs.net Fri Feb 14 09:13:41 2020 From: mpence at grhs.net (Michael S Pence) Date: Fri, 14 Feb 2020 15:13:41 +0000 Subject: [Histonet] Specimens from patient on Chemotherapy Message-ID: Seeking advice from those that know... I am wondering if anyone is handling/processing any lab specimen differently for patients currently taking chemo drugs or shelf 3 or 4 level drugs? We are currently looking and a new chemo spill protocol and this has gotten me to thinking. Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 mpence at grhs.net | www.greatrivermedical.org. www.Facebook.com/GreatRiverHealthSystems | www.Twitter/GreatRiverMed Information in this communication, including attachments, is confidential and intended only for the addressee(s). This communication may contain privileged, confidential, proprietary or trade secret information entitled to protection or exemption from disclosure under law. If you are not an intended recipient, please know that any use, distribution or copying of this communication, or any action taken based on the information in this communication, is unauthorized and may be unlawful. If you received this communication in error, please notify the sender and delete this communication from your device. From carl.hobbs at kcl.ac.uk Fri Feb 14 13:04:30 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 14 Feb 2020 19:04:30 +0000 Subject: [Histonet] Animal and Human Tissues Message-ID: If you are referring to Pwax processing.....no difference imho. I process/have processed tissues from human, axolotl, alligator, mouse, rat, marmoset, quail, chicken, pig, octopus using same schedule. Also fly larvae and earthworm You can see IHC images in the Histonet image archive Best wishes Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From pruegghm at hotmail.com Fri Feb 14 17:51:16 2020 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Fri, 14 Feb 2020 23:51:16 +0000 Subject: [Histonet] Histonet Digest, Vol 195, Issue 10 In-Reply-To: References: Message-ID: I never minded being called in to assist for an aspect of transplants at the U, I figured I was helping a very needy patient. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Thursday, February 13, 2020 11:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 195, Issue 10 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From dunatrsd at sbcglobal.net Mon Feb 17 14:12:05 2020 From: dunatrsd at sbcglobal.net (dusko trajkovic) Date: Mon, 17 Feb 2020 12:12:05 -0800 Subject: [Histonet] Job opening La Jolla Ca References: <2D5E64B4-0777-4673-9205-FC9FE30A47C9.ref@sbcglobal.net> Message-ID: <2D5E64B4-0777-4673-9205-FC9FE30A47C9@sbcglobal.net> Core histoligy job in La Jolla CA, day shift, for a well know academic institution. If interested, please contact me for more info. Dusko Trajkovic Sent from my iPhone From relia1 at earthlink.net Tue Feb 18 08:34:24 2020 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 18 Feb 2020 09:34:24 -0500 Subject: [Histonet] RELIA Hot JOB ALERT Histotech needed Full time permanent DAYS!! in Tampa, FL!! Message-ID: <000001d5e668$849bd4d0$8dd37e70$@earthlink.net> Hi Histopeeps! How are you? I have an exciting new opportunity to share. RELIA has been engaged EXCLUSIVELY for a full time permanent day shift position in the Tampa Bay area. FL lic and ASCP cert are required. Grossing is a plus. For more info please contact me at relia1 at earthlink.net or on my cell at 407-353-5070. Thanks-Pam Right Time, Right Place, Right Move with RELIA! #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Richard.Cartun at hhchealth.org Tue Feb 18 17:12:20 2020 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Tue, 18 Feb 2020 23:12:20 +0000 Subject: [Histonet] Cellient for cell block preparation Message-ID: <9215BD4B0BA1B44D962A71C758B68D2ED457967E@HHCEXCHMB03.hhcsystem.org> Question - What has been your experience with Hologic's Cellient instrument for preparing cell block specimens? I understand that it is very good for pauci-cellular specimens. However, I am concerned about using these preparations for immunohistochemical and DNA/RNA testing. Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Valerie.Hannen at parrishmed.com Wed Feb 19 11:26:34 2020 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Wed, 19 Feb 2020 17:26:34 +0000 Subject: [Histonet] Histology position opening up Message-ID: <04a515b081ef4bb89190732d85c789ff@PMSVRLEX01.parrishmed.local> Good afternoon all, We are hoping to have a full time Histotech job opening here at our facility ( still pending approval from upper management). We have a tech whose last day is February 28th, which leave me being the only Histotechnologist in the whole facility. I am putting "feelers" out now to see if there may be any interest from anyone who may want to apply. Thank you and have a great day. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com From rsrichmond at gmail.com Wed Feb 19 13:46:25 2020 From: rsrichmond at gmail.com (Bob Richmond) Date: Wed, 19 Feb 2020 14:46:25 -0500 Subject: [Histonet] Cellient for cell block preparation In-Reply-To: References: Message-ID: Richard w. Cartun, MS, PhD, at Hartford [CT] Hospital asks: >>What has been your experience with Hologic's Cellient instrument for preparing cell block specimens? I understand that it is very good for pauci-cellular specimens. However, I am concerned about using these preparations for immunohistochemical and DNA/RNA testing.<< This instrument requires fixation of the specimen in ThinPrep's CytoLyt?, an alcohol-based fixative. They specifically note in their documentation that formalin cannot be used. I found a recent (3 years ago) review that addresses the issue: https://www.archivesofpathology.org/doi/pdf/10.5858/arpa.2016-0125-RA Marty Berman was chief resident at Johns Hopkins during my second year in pathology residency there, in 1965. Bob Richmond Samurai Pathologist Maryville TN From Carole.Johnson at uchealth.org Wed Feb 19 14:15:05 2020 From: Carole.Johnson at uchealth.org (Johnson, Carole) Date: Wed, 19 Feb 2020 20:15:05 +0000 Subject: [Histonet] H&E Stainer? Message-ID: Hello All, Our lab is replacing our well-loved and used Sakura Prisma stainer, and I would appreciate input (good or bad) about H&E strainers. We are considering a new Prisma, a Dako CoverStainer, a Leica Spectra, or a Fisher Gemini. Thank you in advance! Carole L Johnson, HT(ASCP)cm, QIHC(ASCP)cm Histotechnologist UCH/Memorial Central Hospital Histology Laboratory 1400 Boulder Street Colorado Springs, CO O 719.365.5204 carole.johnson at uchealth.org CONFIDENTIALITY NOTICE: This message and any attachments may contain privileged and confidential peer review, risk management, and/or quality management information pursuant to the Colorado Professional Review Act, C.R.S. 12-36.5-101, et seq., the Colorado Hospital Licensing law, C.R.S. 25-3-109, the Quality Management Programs law, C.R.S. 25-35-904, and other corresponding provisions of federal and state law. Please maintain the strict confidentiality of this information. If you are not the intended recipient, you are notified that any disclosure, copying, distribution, electronic storage or use of this communication is prohibited. If you received this communication in error, please notify us immediately by e-mail, attaching the original message, and delete the original message from your computer and any network to which your computer is connected. From sandunga59 at yahoo.com Thu Feb 20 10:08:35 2020 From: sandunga59 at yahoo.com (Sondra Delaney) Date: Thu, 20 Feb 2020 10:08:35 -0600 Subject: [Histonet] H&E References: Message-ID: Looking for H&E staining protocols For prostate needle bx, we?re having Issues with uneven staining!! From sandunga59 at yahoo.com Thu Feb 20 10:26:15 2020 From: sandunga59 at yahoo.com (Sondra Delaney) Date: Thu, 20 Feb 2020 10:26:15 -0600 Subject: [Histonet] H&E References: <94DA9DFE-6956-4E0E-A97F-E32274915C04.ref@yahoo.com> Message-ID: <94DA9DFE-6956-4E0E-A97F-E32274915C04@yahoo.com> We?re having issues with uneven staining, prostate needle biopsy. Any suggestions?? Sondra From aperl at caremount.com Thu Feb 20 11:37:42 2020 From: aperl at caremount.com (Perl, Alison) Date: Thu, 20 Feb 2020 17:37:42 +0000 Subject: [Histonet] FW: Cellient for cell block preparation In-Reply-To: <6d8f2528a38148deacafbc6742895de7@MK-EXMB02.mkmg.com> References: <9215BD4B0BA1B44D962A71C758B68D2ED457967E@HHCEXCHMB03.hhcsystem.org> <6d8f2528a38148deacafbc6742895de7@MK-EXMB02.mkmg.com> Message-ID: <696766923719455f9fae8dd5ae1e2e95@MK-EXMB02.mkmg.com> We tried it out, and found that our specimens were apparently TOO paucicellular for even the cellient to make decent cell blocks - after a few months of trialing, we sent it back. Our director had been very hopeful, and was disappointed. Oh, it was also expensive because of the required consumables. Definitely try before you buy! Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at caremount.com -----Original Message----- From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, February 18, 2020 6:12 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] Cellient for cell block preparation Question - What has been your experience with Hologic's Cellient instrument for preparing cell block specimens? I understand that it is very good for pauci-cellular specimens. However, I am concerned about using these preparations for immunohistochemical and DNA/RNA testing. Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tabbott at pennstatehealth.psu.edu Thu Feb 20 11:56:49 2020 From: tabbott at pennstatehealth.psu.edu (Abbott, Tanya) Date: Thu, 20 Feb 2020 17:56:49 +0000 Subject: [Histonet] Giemsa controls Message-ID: <222403bf6a3f471eb1232231bea0ba8f@pennstatehealth.psu.edu> Suggestions for where to buy good Giemsa controls for H. pylori? Thank you in advance! Tanya Tanya G. Abbott Pathology Manager PennState Health St. Joseph Reading Pennsylvania tabbott at pennstatehealth.psu.edu 610-378-2635 From jmyers1 at aol.com Thu Feb 20 14:33:23 2020 From: jmyers1 at aol.com (Joe Myers) Date: Thu, 20 Feb 2020 15:33:23 -0500 Subject: [Histonet] Cellient for cell block preparation References: Message-ID: Dr. Cartun: I?ve worked with the Cellient system in two different labs, with somewhat disappointing results. Although processing specimens with this system does not employ cross-linking fixatives like formalin - which should (in theory) be ?beneficial? to target antigens, in my experience the resulting reactions were less than ideal. Despite multiple attempts, adjusting various protocol parameters (including ?skipping? the HIER step altogether and using both high- and low-pH retrieval solutions), staining intensity/cellular distribution was never as good as it was in formalin-fixed histologic samples. My ?working theory? is that, since all of the antibodies that were tested were characterized against formalin-fixed and heat-retrieved antigens, it simply wasn?t possible to obtain IHC-comparable results with Cellient-processed (i.e. methanol/isopropanol-fixed cytologic) samples. Joe Myers, M.S., CT/QIHC(ASCP) > Subject: [Histonet] Cellient for cell block preparation > > Question - What has been your experience with Hologic's Cellient instrument for preparing cell block specimens? I understand that it is very good for pauci-cellular specimens. However, I am concerned about using these preparations for immunohistochemical and DNA/RNA testing. Thank you! > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 (Office) > (860) 545-2204 (Fax) > Richard.cartun at hhchealth.org From rsrichmond at gmail.com Thu Feb 20 15:03:04 2020 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 20 Feb 2020 16:03:04 -0500 Subject: [Histonet] Giemsa controls In-Reply-To: References: Message-ID: Tanya G. Abbott, Pathology Manager, PennState Health St. Joseph in Reading, Pennsylvania asks: >>Suggestions for where to buy good Giemsa controls for H. pylori?< What I've always found satisfactory is to watch for positives in your own material, evaluate them as possible controls, and use those. It would be nice to have them verified by immunohistochemistry, but really the morphology of each of the two Helicobacter species is sufficient. Bob Richmond Samurai Pathologist Maryville TN From luciuslo9 at gmail.com Fri Feb 21 10:38:03 2020 From: luciuslo9 at gmail.com (lucius lo) Date: Fri, 21 Feb 2020 16:38:03 +0000 Subject: [Histonet] Fwd: Sectioning mice vertebrae and tail In-Reply-To: References: Message-ID: ---------- Forwarded message --------- From: lucius lo Date: Fri, 21 Feb 2020 at 15:46 Subject: Sectioning mice vertebrae and tail To: To whom may concern, I am a University student who currently pursuing a field in histology technician and is having a final year of the histology of bone marrow adipocytes in transgene mice. My question is regarding the sectioning of these fat-rich tissues which torn off easily. My way of sectioning is: 1. Freeze the blocks for an hour in wet ice immersed in distilled water. 2. Trim off the block in one micron while pressing down the lever. 3. Section the slide in five or six microns, then freeze the section again after trimmed off a hundred microns. 4. Obtain another section after placing the block in ice for 30 seconds. The torn off issue are highly corrected after I do step 4. However, *these tissues still appear with tiny holes that only appear after the H&E*. I would like to ask if anyone could share their experiences or solutions in facing tails and vertebrae. Best, Lucius From pruegghm at hotmail.com Sun Feb 23 17:28:48 2020 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Sun, 23 Feb 2020 23:28:48 +0000 Subject: [Histonet] Need a procedure In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F801C1B7269B@HRHEX02-HOS.holyredeemer.local>, Message-ID: ________________________________ From: John Garratt Sent: Thursday, January 23, 2020 10:51 AM To: Terri Braud Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Need a procedure Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good for IHC and does not have the pitfalls of plasma/thrombin. Plasma/thrombin does work well for cell blocks but you will have to consider an ethical and safe source for your plasma. The instructions for using Histogel are in the package insert though I have one comment. Be careful how you warm the Histogel and use a heat block. Do NOT use a microwave since there is a tendency to overheat the gel and you will end up with poor quality IHC. John www.cpqa.ca ??????? Original Message ??????? On Thursday, January 23, 2020 6:10 AM, Terri Braud via Histonet wrote: > Hi fellow Histonetters - I'm in need of some help, please > Background - We currently use agar to capture our scant cell blocks for processing. I am unfamiliar with the Plasma/Thrombin method of cell block preparation and am interested in comparing it to our current method > Request - Could you please send me your procedures for this method, specifically where you purchase your plasma and thrombin and what species are used? > Thanks in advance. Histotechs rock! > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MWhite at mhs.net Mon Feb 24 11:08:45 2020 From: MWhite at mhs.net (White, Marcia) Date: Mon, 24 Feb 2020 17:08:45 +0000 Subject: [Histonet] Histonet Digest, Vol 195, Issue 10 In-Reply-To: References: Message-ID: <7c664ff363cb4b3a9caf995e1522d78f@MHSEXMB03.mhs.net> We do not assist but do go in and perform a frozen section, if this is the assistance they are giving the pathologist then this is normal Marcia White Director Memorial Regional Pathology Services 9581 Premier Parkway, Miramar FL 33025 (954) 276-1855 | Fax:?954-967-7627 ? ? -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, February 13, 2020 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 195, Issue 10 ====================================================================================== THIS EMAIL ORIGINATED FROM OUTSIDE OF MHS. PLEASE EXERCISE CAUTION WITH ATTACHMENTS, LINKS, OR REQUESTED ACTIONS. ====================================================================================== Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=JOaMbd2l0zUJra_1TjFDlA&r=LDt2ZYIvPyjMd5jd_Wmo2w&m=ggK2js3aXcNjr19KMX4LHWdKATB9XZPSAQFDk_BmLCg&s=qayGjygrzR1LFPPom3xD9f2eEhdKIQ7GZIsTB-AL5Ys&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. From lkc0001 at auburn.edu Mon Feb 24 13:41:42 2020 From: lkc0001 at auburn.edu (Lisa Parsons) Date: Mon, 24 Feb 2020 19:41:42 +0000 Subject: [Histonet] Histotech Position open Message-ID: A Histotech position is open at Auburn University (AL). This is for a Veterinary diagnostic and research lab. From LNormington at uwhealth.org Tue Feb 25 10:55:01 2020 From: LNormington at uwhealth.org (Normington Lacy) Date: Tue, 25 Feb 2020 16:55:01 +0000 Subject: [Histonet] Minimum Specimen Size Requirement Message-ID: Does your institution have a policy regarding minimum size requirement for biopsy collection (lung bx, core bx, cardiac, etc)? I will collate all responses. Thank you Lacy From aeck at dh.org Wed Feb 26 05:03:55 2020 From: aeck at dh.org (Eck, Allison) Date: Wed, 26 Feb 2020 11:03:55 +0000 Subject: [Histonet] differences in job descriptions Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64AA7F6D1C6@DH-MAIL02.dhorg.org> I know this topic has probably been addressed many times before and I do not wish to start the certified vs non certified debate again. However, I must ask for those of you who have both working in your lab, do their job descriptions and responsibilities differ between those who are certified and those who are not? If they do differ, what are the differences? Thanks Allison From criley at dpspa.com Wed Feb 26 08:43:58 2020 From: criley at dpspa.com (Charles Riley) Date: Wed, 26 Feb 2020 09:43:58 -0500 Subject: [Histonet] IHC troubleshooting Message-ID: Our pathologists are complaining that chunks of the dermis are missing from IHC slides yet the entire section is present prior to staining. Does anyone have any ideas what could cause the tissue to not adhere to the slides throughout the staining process? We use the Leica Bond stainers. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From john.garratt at ciqc.ca Wed Feb 26 09:21:50 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Wed, 26 Feb 2020 15:21:50 +0000 Subject: [Histonet] IHC troubleshooting In-Reply-To: References: Message-ID: Do you float and pick up your sections from a water bath of distilled water with no adhesive added? If not, doing that will help. John On Wed, Feb 26, 2020 at 6:41 AM, Charles Riley via Histonet wrote: > Our pathologists are complaining that chunks of the dermis are missing from > IHC slides yet the entire section is present prior to staining. > > Does anyone have any ideas what could cause the tissue to not adhere to the > slides throughout the staining process? We use the Leica Bond stainers. > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andijany at hotmail.com Wed Feb 26 10:15:51 2020 From: andijany at hotmail.com (Abdulbasit Andijany) Date: Wed, 26 Feb 2020 16:15:51 +0000 Subject: [Histonet] IHC troubleshooting In-Reply-To: References: , Message-ID: In my experience; If the the specimen is under-fixed or under-processed, the section will be sloughed off during IHC staining. I find proper fixation time and drying the slides at room temperature (before baking them in oven) optimal. Andy Abdulbasit Andijany (Andy) AMLS, BBMS, MSc, CT (ASCP)CM, HTL (ASCPI) King Fahad Armed Forces Hospital, Jeddah, Saudi Arabia On 26 Feb 2020, at 18:35, John Garratt via Histonet > wrote: Do you float and pick up your sections from a water bath of distilled water with no adhesive added? If not, doing that will help. John On Wed, Feb 26, 2020 at 6:41 AM, Charles Riley via Histonet > wrote: Our pathologists are complaining that chunks of the dermis are missing from IHC slides yet the entire section is present prior to staining. Does anyone have any ideas what could cause the tissue to not adhere to the slides throughout the staining process? We use the Leica Bond stainers. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From greg.dobbin at gmail.com Wed Feb 26 12:40:26 2020 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Wed, 26 Feb 2020 14:40:26 -0400 Subject: [Histonet] IHC Troubleshooting Message-ID: Hi Charles, It adhesion problems can arise from several sources, some have already been mentioned. Here are two that I have experienced: 1) Bad slides. Either a manufacturing defect such that the positive charge is insufficient or the slides were somehow compromised after opening them on the bench (perhaps exposed to moisture thus removing the charge). Sometimes a new lot number be better but sometimes switching brands is the quickest fix. 2) Ensuring all water is removed from under the section. I find that with some charged slides (many in fact), water is trapped under the section and does not drain out on standing and does not drain or evaporate when baking at 60C. If the slide with trapped water is put on the instrument with trapped water, the dewax step will lift much of the tissue. Cheers, -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From relia1 at earthlink.net Wed Feb 26 13:03:48 2020 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 26 Feb 2020 14:03:48 -0500 Subject: [Histonet] 15 years THIS WEEK!! And Our latest Job Opportunities!! Message-ID: <000001d5ecd7$79fd12d0$6df73870$@earthlink.net> Hi Histonetters, How are you doing today? This is an exciting week for me! This week we are celebrating RELIA?s 15th year in business. 15 years, 180 months, 782 weeks, 5,475 days, 131,400 hours and loving EVERY one of the 7,884,000 minute of it! RELIA Solutions 2/2005-2/2020 Cheers to 15 more! Thank You!! For the past 15 years I have worked exclusively in the nationwide permanent placement of histology professionals. I have felt so privileged to have helped so many people with their careers and look forward to helping many more! I strive to bring you the best histology opportunities out there. SOME of these are RELIA exclusives MOST of these offer Sign- on Bonuses and/or Relocation Assistance ALL of these Companies offer excellent compensation, benefits and great environments. AND THEY ALL ARE READY TO HIRE!! If You Or Anyone You Know Might Be Interested In Any Of These Positions Please Contact Me. You can reach me by email at relia1 at earthlink.net Toll free at 866-607-3542 or on my cell at 407-353-5070. Here is my Spotlight Opportunity: Lead Histology Tech ? Run Your Own Lab in Beautiful Cheyenne, WY I have been engaged exclusively by a growing Private Gastroenterology Practice in Cheyenne, WY that is in need of a ASCP certified Histotech who is CLIA qualified to gross. This is a Full time DAY shift position and my client offers an excellent pay rate, amazing benefits a beautiful lab to run on your own and one of the most beautiful places to work in the Country!! TEXT ME/ EMAIL ME/ CALL ME for more info! 407-353-5070, relia1 at earthlink.net We Have Exciting Opportunities In: Management: ? California Central CA ? IHC Manager ? Wyoming Cheyenne ? Lead Histotech ? Tennessee Nashville ? 3rd shift Grossing Manager ? Ohio Columbus ? Histology Sales Manager ? Illinois Chicago ? Field Applications Specialist IHC ? Mid East Region Field Applications Specialist IHC Histotechnician/Histotechnologist ? Florida Tampa Bay ? GI 1st or 2nd Shift ? Florida Panama City ? Dermpath Days!! ? North Carolina Wilmington ? Histotech DAYS!! ? Tennessee Kingsport Days Histology Tech Text Me/ Call Me 407-353-5070 /Shoot me an email at relia1 at earthlink.net for details on any of these opportunities! I can also be reached toll free at 866-607-3542. Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From Richard.Cartun at hhchealth.org Wed Feb 26 13:42:39 2020 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Wed, 26 Feb 2020 19:42:39 +0000 Subject: [Histonet] IHC troubleshooting In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2ED457C4C5@HHCEXCHMB03.hhcsystem.org> Are you using some form of antigen retrieval? In addition to what has been discussed previously, antigen retrieval using heat (HIER) can cause tissue loss especially collagen. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, February 26, 2020 9:44 AM To: Histo List Subject: [Histonet] IHC troubleshooting EXTERNAL email from Outside HHC! Do NOT open attachments or click links from unknown senders. Our pathologists are complaining that chunks of the dermis are missing from IHC slides yet the entire section is present prior to staining. Does anyone have any ideas what could cause the tissue to not adhere to the slides throughout the staining process? We use the Leica Bond stainers. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=DSmBsjGihQbMxzBLaqCbr8whSAZVh5SjOAr5sVbspE8&s=Bs1CxoLt5gMxm60Er8yfxoTB45uLgwau1GA_3MK5-jQ&e= Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From relia1 at earthlink.net Wed Feb 26 14:23:44 2020 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 26 Feb 2020 15:23:44 -0500 Subject: [Histonet] 15 years THIS WEEK!! And Our latest Job Opportunities!! Message-ID: <0aaa01d5ece2$a4a8fd40$edfaf7c0$@earthlink.net> Hi Histonetters, How are you doing today? This is an exciting week for me! This week we are celebrating RELIA?s 15th year in business. 15 years, 180 months, 782 weeks, 5,475 days, 131,400 hours and loving EVERY one of the 7,884,000 minute of it! RELIA Solutions 2/2005-2/2020 Cheers to 15 more! Thank You!! For the past 15 years I have worked exclusively in the nationwide permanent placement of histology professionals. I have felt so privileged to have helped so many people with their careers and look forward to helping many more! I strive to bring you the best histology opportunities out there. SOME of these are RELIA exclusives MOST of these offer Sign- on Bonuses and/or Relocation Assistance ALL of these Companies offer excellent compensation, benefits and great environments. AND THEY ALL ARE READY TO HIRE!! If You Or Anyone You Know Might Be Interested In Any Of These Positions Please Contact Me. You can reach me by email at relia1 at earthlink.net Toll free at 866-607-3542 or on my cell at 407-353-5070. Here is my Spotlight Opportunity: Lead Histology Tech ? Run Your Own Lab in Beautiful Cheyenne, WY I have been engaged exclusively by a growing Private Gastroenterology Practice in Cheyenne, WY that is in need of a ASCP certified Histotech who is CLIA qualified to gross. 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Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From criley at dpspa.com Wed Feb 26 16:09:14 2020 From: criley at dpspa.com (Charles Riley) Date: Wed, 26 Feb 2020 17:09:14 -0500 Subject: [Histonet] Poll question Message-ID: How often does everyone clean their water-bath? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From cls71877 at gmail.com Wed Feb 26 16:59:31 2020 From: cls71877 at gmail.com (Cristi Rigazio) Date: Wed, 26 Feb 2020 17:59:31 -0500 Subject: [Histonet] Poll question In-Reply-To: References: Message-ID: <0F2999EB-7484-4434-BD86-139E9CCE62B2@gmail.com> Do you mean between specimens or per shift? Thanks Cristi Sent from my iPhone > On Feb 26, 2020, at 5:24 PM, Charles Riley via Histonet wrote: > > ?How often does everyone clean their water-bath? > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Katie at PuyallupDerm.com Thu Feb 27 14:21:48 2020 From: Katie at PuyallupDerm.com (Katie) Date: Thu, 27 Feb 2020 12:21:48 -0800 Subject: [Histonet] External alarm for Tissue-Tek VIP E300 Message-ID: <4CCAEE612BC94A01AB7EF2DFE5557EBD@LabHP> I am looking to get an external alarm for our tissue processor. Any suggestions? Thanks so much! Katie Riley-Hamilton Technical Supervisor of Dermatopathology Puyallup Dermatology Clinic katie at puyallupderm.com From john.garratt at ciqc.ca Thu Feb 27 15:01:06 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 27 Feb 2020 21:01:06 +0000 Subject: [Histonet] External alarm for Tissue-Tek VIP E300 In-Reply-To: <4CCAEE612BC94A01AB7EF2DFE5557EBD@LabHP> References: <4CCAEE612BC94A01AB7EF2DFE5557EBD@LabHP> Message-ID: There are wiring ports on the E3000 for an external alarm. You have to decide where the alarm can be monitored 24/7 at your site. If you mean a wireless monitoring system, and these are systems, take a look at Thermo. https://www.thermofisher.com/ca/en/home/life-science/lab-equipment/wireless-monitoring-systems.html John www.cpqa.ca ??????? Original Message ??????? On Thursday, February 27, 2020 12:21 PM, Katie via Histonet wrote: > I am looking to get an external alarm for our tissue processor. Any > suggestions? Thanks so much! > > Katie Riley-Hamilton > Technical Supervisor of Dermatopathology > > Puyallup Dermatology Clinic > > katie at puyallupderm.com > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akuvetpatoloji at gmail.com Fri Feb 28 03:51:55 2020 From: akuvetpatoloji at gmail.com (Vet Patoloji) Date: Fri, 28 Feb 2020 12:51:55 +0300 Subject: [Histonet] olympus Dp20 camera CD Message-ID: Hello Histonetters, Because we have lost what we have, I need a CD image of the Cell A program to be used in the Olympus Dp20 camera. I will be grateful if you could help me. Thank you. -- Dr. M. Fatih Bozkurt AK? Veteriner Fak?ltesi Analiz ve Te?his Laboratuvar? Patoloji Birimi - Afyonkarahisar From Nancy_Schmitt at pa-ucl.com Fri Feb 28 06:03:48 2020 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Fri, 28 Feb 2020 12:03:48 +0000 Subject: [Histonet] IHC Troubleshooting and Water Baths Message-ID: Hi Charles- IHC staining - We also use the Leica Bond staining system and had tissue fall off in the beginning. We blot the slides to remove any additional water and then place in drying oven for 30 minutes at 60 before placing on the stainer. Water baths - at the end of each day the water is dumped, the bath is rinsed with bleach solution, rinsed with water and dried. Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, February 26, 2020 9:44 AM To: Histo List Subject: [Histonet] IHC troubleshooting EXTERNAL email from Outside HHC! Do NOT open attachments or click links from unknown senders. Our pathologists are complaining that chunks of the dermis are missing from IHC slides yet the entire section is present prior to staining. Does anyone have any ideas what could cause the tissue to not adhere to the slides throughout the staining process? We use the Leica Bond stainers. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs Message: 5 Date: Wed, 26 Feb 2020 17:09:14 -0500 From: Charles Riley To: Histo List Subject: [Histonet] Poll question Message-ID: Content-Type: text/plain; charset="UTF-8" How often does everyone clean their water-bath? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From Blanca.Lopez at UTSouthwestern.edu Fri Feb 28 10:59:22 2020 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Fri, 28 Feb 2020 16:59:22 +0000 Subject: [Histonet] IHC question Message-ID: <1AB0FFAE-AC78-4289-A3B3-75F9DB4909BD@UTSouthwestern.edu> I have a question regarding ihc I?m running some ihc using Dako linker 48, this gave me a notice of short reagent but when I made it my time was off and suspended. The slides are just being on peroxide block for 5 min. Can I just reload them again? Is this will not affect my staining Ir what is you suggestion Thanks Blanca Lopez HT(ASCP) Histotechnologist UT Southwestern Medical Center Harold C Simmons Comprehensive Cancer Center Pathology Lab NB5-102 214-648-7598 blanca.lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today.