From Sharon.Wheeler at lhsc.on.ca Tue Dec 1 08:07:07 2020 From: Sharon.Wheeler at lhsc.on.ca (Sharon Wheeler) Date: Tue, 1 Dec 2020 14:07:07 +0000 Subject: [Histonet] Question for Histonet Message-ID: I was wondering if other facilities have the same issue as us? We have excess breakage (brittleness) of the solution reservoirs (680 mL) for our PRISMA stainer. We clean the dirty reservoirs in an automatic dishwasher with a 18 minute dry cycle at 90 degrees. How do other places clean their baskets? Has anybody else had similar experience??? Thanks, Sharon Senior Technologist Surgical & Ancillary Pathology Pathology and Laboratory Medicine London Health Sciences Centre and St. Joseph's Health Care London University Hospital, Rm A3-236 TEL: 519-685-8500, ext. 34711 E-mail: Sharon.wheeler at lhsc.on.ca This email is directed in confidence solely to the person named above and may contain confidential, privileged or personal health information. Please be aware that this email may also be released to members of the public under Ontario's Freedom of Information and Protection of Privacy Act if required. Review, distribution, or disclosure of this email by anyone other than the person(s) for whom it was originally intended is strictly prohibited. If you are not an intended recipient, please notify the sender immediately via a return email and destroy all copies of the original message. Thank you for your cooperation. From mward at wakehealth.edu Fri Dec 4 07:55:20 2020 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Fri, 4 Dec 2020 13:55:20 +0000 Subject: [Histonet] "Floaters" in surgical or cytology specimens Message-ID: I am posting this question for a colleague in our Cytology department. How often do you see floaters on surgical or cytology specimens? Obviously we would never want to see any type of carryover but is there a standard rate published somewhere that he can reference? Thanks in advance for your help. Martha Ward, MT ASCP, QIHC Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center From jwwalker at rrmc.org Fri Dec 4 11:41:11 2020 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Fri, 4 Dec 2020 17:41:11 +0000 Subject: [Histonet] "Floaters" in surgical or cytology specimens In-Reply-To: References: Message-ID: https://academic.oup.com/ajcp/article/136/5/767/1766314 "Floaters represent a potential source of diagnostic error and occur in 0.01% to 1.2% of slides. Pick up of floaters from the water bath appears most common (?60%). Floaters in only 1 level and mismatch with the specimen tissue type are clues to the extraneous nature of the floater." Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology and Interim Phlebotomy Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Martha Ward-Pathology via Histonet Sent: Friday, December 4, 2020 8:55 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] "Floaters" in surgical or cytology specimens [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. I am posting this question for a colleague in our Cytology department. How often do you see floaters on surgical or cytology specimens? Obviously we would never want to see any type of carryover but is there a standard rate published somewhere that he can reference? Thanks in advance for your help. Martha Ward, MT ASCP, QIHC Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!I87qwjxLstg3H_X5!vtJNIWgmSiJpyLbSct_WD7kUYBMBOk43t6WiqJfWPo6GJv5urHibw3NEp3Ztj3I$ [https://www.rrmc.org/app/files/public/3159/ValesEmailSig2020.jpg] From idimenstein at hotmail.com Sun Dec 6 12:42:45 2020 From: idimenstein at hotmail.com (Izak Dimenstein) Date: Sun, 6 Dec 2020 18:42:45 +0000 Subject: [Histonet] Histonet Digest, Vol 205, Issue 2 In-Reply-To: References: Message-ID: In my experience and opinion, grossing and embedding are the main sources of extraneous tissues ("floaters"). This issue is discussed in detail in the section "Root Cause Analysis for floaters prevention during grossing and embedding in surgical pathology histology laboratory" in my book Grossing Technology pp. 254-265. Izak Dimenstein ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Friday, December 4, 2020 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 205, Issue 2 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From tbraud at holyredeemer.com Mon Dec 7 07:42:09 2020 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 7 Dec 2020 13:42:09 +0000 Subject: [Histonet] Floaters Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C4C3B05F@HRHEX02-HOS.holyredeemer.local> 60% of floaters from the water bath? I find that really hard to believe. The Gephardt and Zarbo CAP study from 1996 showed reported the results of a Q-Probes study of 275 laboratories and documented a frequency of contamination of between 0.6% and 2.9%, depending on the study method. Their study demonstrated the rate of extraneous tissue contamination was higher for blocks than for slides and higher in a retrospective review than in a prospective study. So in other words, when people knew they were being studied, they were more careful and the contamination rate went down, but in retrospect, the majority of floaters occurred in the blocks, not the water bath. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor HNL Labs, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. "Floaters" in surgical or cytology specimens (Martha Ward-Pathology) 2. Re: "Floaters" in surgical or cytology specimens (Joe W. Walker, Jr.) ---------------------------------------------------------------------- Message: 1 Date: Fri, 4 Dec 2020 13:55:20 +0000 From: Martha Ward-Pathology To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] "Floaters" in surgical or cytology specimens Message-ID: Content-Type: text/plain; charset="us-ascii" I am posting this question for a colleague in our Cytology department. How often do you see floaters on surgical or cytology specimens? Obviously we would never want to see any type of carryover but is there a standard rate published somewhere that he can reference? Thanks in advance for your help. Martha Ward, MT ASCP, QIHC Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center ------------------------------ Message: 2 Date: Fri, 4 Dec 2020 17:41:11 +0000 From: "Joe W. Walker, Jr." To: Martha Ward-Pathology Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] "Floaters" in surgical or cytology specimens Message-ID: Content-Type: text/plain; charset="utf-8" https://academic.oup.com/ajcp/article/136/5/767/1766314 "Floaters represent a potential source of diagnostic error and occur in 0.01% to 1.2% of slides. Pick up of floaters from the water bath appears most common (?60%). Floaters in only 1 level and mismatch with the specimen tissue type are clues to the extraneous nature of the floater." Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology and Interim Phlebotomy Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Martha Ward-Pathology via Histonet Sent: Friday, December 4, 2020 8:55 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] "Floaters" in surgical or cytology specimens [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. I am posting this question for a colleague in our Cytology department. How often do you see floaters on surgical or cytology specimens? Obviously we would never want to see any type of carryover but is there a standard rate published somewhere that he can reference? Thanks in advance for your help. Martha Ward, MT ASCP, QIHC Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!I87qwjxLstg3H_X5!vtJNIWgmSiJpyLbSct_WD7kUYBMBOk43t6WiqJfWPo6GJv5urHibw3NEp3Ztj3I$ [https://www.rrmc.org/app/files/public/3159/ValesEmailSig2020.jpg] ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 205, Issue 2 **************************************** From jwwalker at rrmc.org Mon Dec 7 11:15:17 2020 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Mon, 7 Dec 2020 17:15:17 +0000 Subject: [Histonet] Floaters In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F801C4C3B05F@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F801C4C3B05F@HRHEX02-HOS.holyredeemer.local> Message-ID: Don?t shoot the messenger. :) Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology and Interim Phlebotomy Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790? F 802.747.6525 joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Terri Braud via Histonet Sent: Monday, December 7, 2020 8:42 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Floaters [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. 60% of floaters from the water bath? I find that really hard to believe. The Gephardt and Zarbo CAP study from 1996 showed reported the results of a Q-Probes study of 275 laboratories and documented a frequency of contamination of between 0.6% and 2.9%, depending on the study method. Their study demonstrated the rate of extraneous tissue contamination was higher for blocks than for slides and higher in a retrospective review than in a prospective study. So in other words, when people knew they were being studied, they were more careful and the contamination rate went down, but in retrospect, the majority of floaters occurred in the blocks, not the water bath. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor HNL Labs, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. "Floaters" in surgical or cytology specimens (Martha Ward-Pathology) 2. Re: "Floaters" in surgical or cytology specimens (Joe W. Walker, Jr.) ---------------------------------------------------------------------- Message: 1 Date: Fri, 4 Dec 2020 13:55:20 +0000 From: Martha Ward-Pathology To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] "Floaters" in surgical or cytology specimens Message-ID: Content-Type: text/plain; charset="us-ascii" I am posting this question for a colleague in our Cytology department. How often do you see floaters on surgical or cytology specimens? Obviously we would never want to see any type of carryover but is there a standard rate published somewhere that he can reference? Thanks in advance for your help. Martha Ward, MT ASCP, QIHC Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center ------------------------------ Message: 2 Date: Fri, 4 Dec 2020 17:41:11 +0000 From: "Joe W. Walker, Jr." To: Martha Ward-Pathology Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] "Floaters" in surgical or cytology specimens Message-ID: Content-Type: text/plain; charset="utf-8" https://urldefense.com/v3/__https://academic.oup.com/ajcp/article/136/5/767/1766314__;!!I87qwjxLstg3H_X5!rJ2yq9KcDC2PooORZtJvXi4R8vHOIg5tak39dSSWFLa5SL1M73A18pgYpUvPASA$ "Floaters represent a potential source of diagnostic error and occur in 0.01% to 1.2% of slides. Pick up of floaters from the water bath appears most common (?60%). Floaters in only 1 level and mismatch with the specimen tissue type are clues to the extraneous nature of the floater." Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology and Interim Phlebotomy Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewalker at rrmc.org, http://www.rrmc.org -----Original Message----- From: Martha Ward-Pathology via Histonet Sent: Friday, December 4, 2020 8:55 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] "Floaters" in surgical or cytology specimens [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. I am posting this question for a colleague in our Cytology department. How often do you see floaters on surgical or cytology specimens? Obviously we would never want to see any type of carryover but is there a standard rate published somewhere that he can reference? Thanks in advance for your help. Martha Ward, MT ASCP, QIHC Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!I87qwjxLstg3H_X5!vtJNIWgmSiJpyLbSct_WD7kUYBMBOk43t6WiqJfWPo6GJv5urHibw3NEp3Ztj3I$ [https://www.rrmc.org/app/files/public/3159/ValesEmailSig2020.jpg] ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!I87qwjxLstg3H_X5!rJ2yq9KcDC2PooORZtJvXi4R8vHOIg5tak39dSSWFLa5SL1M73A18pgYIVvlKVk$ ------------------------------ End of Histonet Digest, Vol 205, Issue 2 **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!I87qwjxLstg3H_X5!rJ2yq9KcDC2PooORZtJvXi4R8vHOIg5tak39dSSWFLa5SL1M73A18pgYIVvlKVk$ From john.garratt at ciqc.ca Mon Dec 7 11:23:37 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Mon, 07 Dec 2020 17:23:37 +0000 Subject: [Histonet] Floaters In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F801C4C3B05F@HRHEX02-HOS.holyredeemer.local> Message-ID: Interesting thread. Thanks for kicking it off. Does anybody have a reference that cites diagnostic errors caused by floaters / contamination from other specimens? John On Mon, Dec 7, 2020 at 9:15 AM, Joe W. Walker, Jr. via Histonet wrote: > Don?t shoot the messenger. :) > > Joe W. Walker, Jr. MS, SCT(ASCP) > Anatomical Pathology and Interim Phlebotomy Manager > Rutland Regional Medical Center > 160 Allen Street, Rutland, VT 05701 > P 802.747.1790 F 802.747.6525 > joewalker at rrmc.org, www.rrmc.org > > -----Original Message----- > From: Terri Braud via Histonet > Sent: Monday, December 7, 2020 8:42 AM > To: 'histonet at lists.utsouthwestern.edu' > Subject: Re: [Histonet] Floaters > > [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. > > 60% of floaters from the water bath? I find that really hard to believe. > The Gephardt and Zarbo CAP study from 1996 showed reported the results of a Q-Probes study of 275 laboratories and documented a frequency of contamination of between 0.6% and 2.9%, depending on the study method. Their study demonstrated the rate of extraneous tissue contamination was higher for blocks than for slides and higher in a retrospective review than in a prospective study. So in other words, when people knew they were being studied, they were more careful and the contamination rate went down, but in retrospect, the majority of floaters occurred in the blocks, not the water bath. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > HNL Labs, Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > Today's Topics: > > 1. "Floaters" in surgical or cytology specimens > (Martha Ward-Pathology) > 2. Re: "Floaters" in surgical or cytology specimens > (Joe W. Walker, Jr.) > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 4 Dec 2020 13:55:20 +0000 > From: Martha Ward-Pathology > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] "Floaters" in surgical or cytology specimens > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > I am posting this question for a colleague in our Cytology department. How often do you see floaters on surgical or cytology specimens? Obviously we would never want to see any type of carryover but is there a standard rate published somewhere that he can reference? > > Thanks in advance for your help. > > Martha Ward, MT ASCP, QIHC > Manager, Molecular Diagnostics Lab > Wake Forest Baptist Medical Center > > ------------------------------ > > Message: 2 > Date: Fri, 4 Dec 2020 17:41:11 +0000 > From: "Joe W. Walker, Jr." > To: Martha Ward-Pathology > Cc: "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] "Floaters" in surgical or cytology specimens > Message-ID: > > > Content-Type: text/plain; charset="utf-8" > > https://urldefense.com/v3/__https://academic.oup.com/ajcp/article/136/5/767/1766314__;!!I87qwjxLstg3H_X5!rJ2yq9KcDC2PooORZtJvXi4R8vHOIg5tak39dSSWFLa5SL1M73A18pgYpUvPASA$ > > "Floaters represent a potential source of diagnostic error and occur in 0.01% to 1.2% of slides. Pick up of floaters from the water bath appears most common (?60%). Floaters in only 1 level and mismatch with the specimen tissue type are clues to the extraneous nature of the floater." > > Joe W. Walker, Jr. MS, SCT(ASCP) > Anatomical Pathology and Interim Phlebotomy Manager Rutland Regional Medical Center > 160 Allen Street, Rutland, VT 05701 > P 802.747.1790 F 802.747.6525 > joewalker at rrmc.org, http://www.rrmc.org > > -----Original Message----- > From: Martha Ward-Pathology via Histonet > Sent: Friday, December 4, 2020 8:55 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] "Floaters" in surgical or cytology specimens > > [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. > > I am posting this question for a colleague in our Cytology department. How often do you see floaters on surgical or cytology specimens? Obviously we would never want to see any type of carryover but is there a standard rate published somewhere that he can reference? > > Thanks in advance for your help. > > Martha Ward, MT ASCP, QIHC > Manager, Molecular Diagnostics Lab > Wake Forest Baptist Medical Center > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!I87qwjxLstg3H_X5!vtJNIWgmSiJpyLbSct_WD7kUYBMBOk43t6WiqJfWPo6GJv5urHibw3NEp3Ztj3I$ > [https://www.rrmc.org/app/files/public/3159/ValesEmailSig2020.jpg] > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!I87qwjxLstg3H_X5!rJ2yq9KcDC2PooORZtJvXi4R8vHOIg5tak39dSSWFLa5SL1M73A18pgYIVvlKVk$ > > ------------------------------ > > End of Histonet Digest, Vol 205, Issue 2 > **************************************** > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!I87qwjxLstg3H_X5!rJ2yq9KcDC2PooORZtJvXi4R8vHOIg5tak39dSSWFLa5SL1M73A18pgYIVvlKVk$ > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rmccormick10 at yahoo.com Mon Dec 7 11:39:32 2020 From: rmccormick10 at yahoo.com (Rhonda McCormick) Date: Mon, 7 Dec 2020 17:39:32 +0000 (UTC) Subject: [Histonet] Crusting lesions and thick cattle skin References: <1274842482.5351927.1607362772340.ref@mail.yahoo.com> Message-ID: <1274842482.5351927.1607362772340@mail.yahoo.com> Hi!I'm looking for recommendations for cutting FFPE blocks that contain thick cattle hide/skin and crusting lesions. What do you all use to soften this kind of tissue?? Our lab has used Millifex, fabric softener, and nair.? We read ammonia may work to rehydrate the tissue enough to get a decent section. Has anyone tried that?Thanks in advance for your help!Rhonda From wilfong1923 at gmail.com Mon Dec 7 12:21:31 2020 From: wilfong1923 at gmail.com (John Frazier) Date: Mon, 7 Dec 2020 13:21:31 -0500 Subject: [Histonet] Floaters Message-ID: Here is a reference link to floaters. My experience with tissue floaters is that most come from staining baths. However, some manufacturers of stainers claim there is significance with these floaters. My experience is that Pathologists read through the floaters and for the most part they don't mind. My concern for floaters that pose a real threat to an accurate diagnosis come from the block. Created either at grossing with contaminated grossing tools or embedding. https://meridian.allenpress.com/aplm/article/133/6/973/64098/Tissue-Floaters-and-Contaminants-in-the-Histology *John Frazier* *MT(ASCP), MBA* *Lean 6 Six Sigma Black Belt* *Healthcare Consultant* *704-847-0566* *wilfong1923 at gmail.com * From relia1 at earthlink.net Tue Dec 8 11:09:31 2020 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 8 Dec 2020 12:09:31 -0500 Subject: [Histonet] There's No Place Like Home for the Holidays! Message-ID: <007e01d6cd84$e4faff00$aef0fd00$@earthlink.net> Hello Histopeeps, I hope you are enjoying a wonderful holiday season and are looking forward to a healthy and happy 2021! As the subject line in this email and the popular holiday carol say. "There's no place like home for the holidays" **If Not THIS year; Can I get you HOME for NEXT year?** Histopeeps, I sent a similar note to my clients pointing out that there may be some people looking to be home permanently by next Christmas and am in discussion with clients all over the U.S. about positions. If you are someone who might consider a job change with or without relocating in 2021 please drop me a quick email with your desired location. My phone has been ringing off the hook!! I need to know: * Where you are! * What you want to do! * Where you want to be! If you are looking immediately or interested in ANY of the following areas call my cell at 407-353-5070!! Or drop me a quick email at relia1 at earthlink.net I have exciting opportunities in the following areas: Histotechnician/ Technologist Opportunities: . California . Colorado . Florida . North Carolina . South Carolina . New York . Wisconsin . Kentucky My clients say you can start NOW or AFTER the holidays YOUR CHOICE! Happy Holidays !!!! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Kelly.Pairan at nationwidechildrens.org Tue Dec 8 14:46:44 2020 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Tue, 8 Dec 2020 20:46:44 +0000 Subject: [Histonet] Maximum and Minimum Temperatures on Embedding Center Paraffin Tanks Message-ID: <6daf3e9762a24098aa29f2b90ad33000@l1perdwmbx01.childrensroot.net> Good Afternoon, Recently we starting taking the daily maximum and minimum temperature ranges for our embedding center paraffin tanks. The melting point of our wax is 56oC and our current temperature range is 58 to 65oC. The problem we are having is that when we add wax, the temp dips below the range until it melts. If we turn it up, it exceeds the max range. Any suggestions? Thanks, Kelly From lubbockcat at mail.com Tue Dec 8 15:22:02 2020 From: lubbockcat at mail.com (lubbockcat at mail.com) Date: Tue, 8 Dec 2020 22:22:02 +0100 Subject: [Histonet] (no subject) Message-ID: Sent using the mail.com mail app From ewj at pigs.ag Tue Dec 8 15:25:32 2020 From: ewj at pigs.ag (E. Wayne Johnson) Date: Wed, 9 Dec 2020 05:25:32 +0800 Subject: [Histonet] Maximum and Minimum Temperatures on Embedding Center Paraffin Tanks In-Reply-To: <6daf3e9762a24098aa29f2b90ad33000@l1perdwmbx01.childrensroot.net> References: <6daf3e9762a24098aa29f2b90ad33000@l1perdwmbx01.childrensroot.net> Message-ID: <751adf90-0b98-a0ac-60c4-98164a222bb9@pigs.ag> You could warm the paraffin in an oven overnight to melt it so that the heat of fusion is not extracted from the embedding center paraffin tanks when it is added as melted paraffin at 58-65 degrees. Pairan, Kelly via Histonet wrote: > Good Afternoon, > Recently we starting taking the daily maximum and minimum temperature ranges for our embedding center paraffin tanks. The melting point of our wax is 56oC and our current temperature range is 58 to 65oC. The problem we are having is that when we add wax, the temp dips below the range until it melts. If we turn it up, it exceeds the max range. Any suggestions? > > Thanks, > Kelly > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Timothy.Morken at ucsf.edu Tue Dec 8 16:17:09 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 8 Dec 2020 22:17:09 +0000 Subject: [Histonet] Maximum and Minimum Temperatures on Embedding Center Paraffin Tanks In-Reply-To: <751adf90-0b98-a0ac-60c4-98164a222bb9@pigs.ag> References: <6daf3e9762a24098aa29f2b90ad33000@l1perdwmbx01.childrensroot.net> <751adf90-0b98-a0ac-60c4-98164a222bb9@pigs.ag> Message-ID: Kelly, You are not going to stay within the temp ranges if putting pellets directly into the embedding center tank. A couple solutions: 1) write into your procedure that the temperature will go down when pellets are added and that once melted should be in whatever range it calls for (and don't turn it up, because that means you have to keep adjusting it and someone may forget and as you say, exceed the temperature - you just need to put in far enough ahead to let it melt. Or, 2) Get an bulk paraffin dispenser and melt the paraffin in that, then draw off liquid paraffin for your embedding center. This is pretty common for tissue processor and embedding centers to avoid having to use them to melt the paraffin. Gets you back to work faster as well. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: E. Wayne Johnson via Histonet Sent: Tuesday, December 08, 2020 1:26 PM To: Pairan, Kelly ; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Maximum and Minimum Temperatures on Embedding Center Paraffin Tanks You could warm the paraffin in an oven overnight to melt it so that the heat of fusion is not extracted from the embedding center paraffin tanks when it is added as melted paraffin at 58-65 degrees. Pairan, Kelly via Histonet wrote: > Good Afternoon, > Recently we starting taking the daily maximum and minimum temperature ranges for our embedding center paraffin tanks. The melting point of our wax is 56oC and our current temperature range is 58 to 65oC. The problem we are having is that when we add wax, the temp dips below the range until it melts. If we turn it up, it exceeds the max range. Any suggestions? > > Thanks, > Kelly > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7 > cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=4_M_9rKMFL-Sh7pBndIIL-bv0 > oW9Qdhfz4CMKoyUJz0&s=sXXQcK8dPXYAYVBhgQQkD_qJtcBV3mOUyb7ehqCprQg&e= > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=4_M_9rKMFL-Sh7pBndIIL-bv0oW9Qdhfz4CMKoyUJz0&s=sXXQcK8dPXYAYVBhgQQkD_qJtcBV3mOUyb7ehqCprQg&e= From mward at wakehealth.edu Thu Dec 10 12:44:49 2020 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Thu, 10 Dec 2020 18:44:49 +0000 Subject: [Histonet] Question about accessioning outside consult cases Message-ID: We are in the process of switching to AP Beaker and in the midst of the build. My question involves how others are handling outside consult surgical and/or cytology cases. Do you assign them an unique number that identifies them as a consult as opposed to an inside case - SO20-XXXX verses S20-XXXX? We currently assign them as just another surgical case but I know some places do have outside consult designations. What is everyone doing? Thanks in advance for your input. Martha Ward, MT ASCP (QIHC) Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 From lesraff at gmail.com Thu Dec 10 12:49:16 2020 From: lesraff at gmail.com (Lester Raff) Date: Thu, 10 Dec 2020 12:49:16 -0600 Subject: [Histonet] Question about accessioning outside consult cases In-Reply-To: References: Message-ID: We identify our outside surgical consults as OC, for example OC20-00010 Les Raff On Thu, Dec 10, 2020 at 12:45 PM Martha Ward-Pathology via Histonet < histonet at lists.utsouthwestern.edu> wrote: > We are in the process of switching to AP Beaker and in the midst of the > build. My question involves how others are handling outside consult > surgical and/or cytology cases. Do you assign them an unique number that > identifies them as a consult as opposed to an inside case - SO20-XXXX > verses S20-XXXX? We currently assign them as just another surgical case > but I know some places do have outside consult designations. > > What is everyone doing? > > Thanks in advance for your input. > > Martha Ward, MT ASCP (QIHC) > Manager, Molecular Diagnostics Lab > Wake Forest Baptist Medical Center > Winston-Salem, NC 27157 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Timothy.Morken at ucsf.edu Thu Dec 10 13:14:37 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 10 Dec 2020 19:14:37 +0000 Subject: [Histonet] Question about accessioning outside consult cases In-Reply-To: References: Message-ID: Martha, we give it the same number sequence as any other surgical or cytology case, but we can identify our cases by "Specimen Class" so consults get a specimen class according to the type of consult - cyto, cytogyn, surgical, etc. The specimen class is printed on the labels for all materials. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Martha Ward-Pathology via Histonet Sent: Thursday, December 10, 2020 10:45 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Question about accessioning outside consult cases We are in the process of switching to AP Beaker and in the midst of the build. My question involves how others are handling outside consult surgical and/or cytology cases. Do you assign them an unique number that identifies them as a consult as opposed to an inside case - SO20-XXXX verses S20-XXXX? We currently assign them as just another surgical case but I know some places do have outside consult designations. What is everyone doing? Thanks in advance for your input. Martha Ward, MT ASCP (QIHC) Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=603LFlW434sZQ9xqD7x1rQ7R6rwp5OXmm7_AOPYejrI&s=vAX2gJEr1m1a0cn4ivBSi2PnoEiK6Zm8vgMYiDX7GpI&e= From Scott.Lindrud at carrishealth.com Fri Dec 11 12:44:21 2020 From: Scott.Lindrud at carrishealth.com (Lindrud, Scott) Date: Fri, 11 Dec 2020 18:44:21 +0000 Subject: [Histonet] Question about accessioning outside consult cases In-Reply-To: References: Message-ID: Hi Martha, We have been using Beaker for over 2 years now. We created another accession that identifies cases that are being sent to us as a consult/referral (both Cyto and Histo). It works pretty well for us. Scott Scott A. Lindrud, MLS(ASCP)CT | Histopathology Technical Specalist/Cytotechnologist P: 320-231-4520 F: 320-231-4503 scott.lindrud at carrishealth.com Carris-Health Rice Memorial Hospital 301 Becker Ave SW Willmar, MN 56201 -----Original Message----- From: Martha Ward-Pathology Sent: Thursday, December 10, 2020 12:45 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Question about accessioning outside consult cases We are in the process of switching to AP Beaker and in the midst of the build. My question involves how others are handling outside consult surgical and/or cytology cases. Do you assign them an unique number that identifies them as a consult as opposed to an inside case - SO20-XXXX verses S20-XXXX? We currently assign them as just another surgical case but I know some places do have outside consult designations. What is everyone doing? Thanks in advance for your input. Martha Ward, MT ASCP (QIHC) Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center Winston-Salem, NC 27157 Confidentiality Notice: This e-mail and any attachment may contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this transmission in error, please notify the sender immediately, reply to this transmission, or contact the CentraCare Information Systems Network Security staff by calling the IS Help Desk for assistance at 320-251-2700, ext. 54540, and delete these documents. From tbraud at holyredeemer.com Fri Dec 11 13:03:37 2020 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 11 Dec 2020 19:03:37 +0000 Subject: [Histonet] Ouside Consults Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C4C3BD7F@HRHEX02-HOS.holyredeemer.local> Interesting question - Here, as previous places I've worked, we accession outside surgical consults with one of our routine Surgical numbers, and outside Cytological consults with a routine Cytology accession number. If we receive multiple mixed cases, they are all reported as a Surgical Consult. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor HNL Labs, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Question about accessioning outside consult cases (Martha Ward-Pathology) From criley at dpspa.com Mon Dec 14 11:12:54 2020 From: criley at dpspa.com (Charles Riley) Date: Mon, 14 Dec 2020 17:12:54 +0000 Subject: [Histonet] Film coverslipping adjustments Message-ID: We are running into an issue with our automated film coverslipper. The borders where the film meets the slide show small distortions (possibly air bubbles or incompletely melted adhesive). Has anyone ran into this issue before and could you recommend adjustments or troubleshooting methods to try to clean up the slides appearance? From nmargaryan88 at gmail.com Fri Dec 18 15:53:35 2020 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Fri, 18 Dec 2020 15:53:35 -0600 Subject: [Histonet] concentration of Ammonia water Message-ID: Hi, Please help me to remember what concentration of Ammonia water need for microtome hard paraffin blocks? Thanks in advance Naira From nmargaryan88 at gmail.com Mon Dec 21 08:37:24 2020 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Mon, 21 Dec 2020 08:37:24 -0600 Subject: [Histonet] concentration of Ammonia water In-Reply-To: References: Message-ID: Thanks everybody and have a nice day! Naira On Fri, Dec 18, 2020 at 3:53 PM Naira Margaryan wrote: > Hi, > > Please help me to remember what concentration of Ammonia water need for > microtome hard paraffin blocks? > > Thanks in advance > Naira > From criley at dpspa.com Mon Dec 21 12:57:57 2020 From: criley at dpspa.com (Charles Riley) Date: Mon, 21 Dec 2020 18:57:57 +0000 Subject: [Histonet] Detection systems for IHC's Message-ID: DOes anyone know what the comparison detection kit for the Xfinity Matrix from Biogenex would be in relation to the Bond Refine detection kits? From plucas at biopath.org Tue Dec 22 08:36:28 2020 From: plucas at biopath.org (Paula) Date: Tue, 22 Dec 2020 06:36:28 -0800 Subject: [Histonet] Paraffin Preference Message-ID: <004601d6d86f$d611ab60$82350220$@biopath.org> Hi everyone..good morning. I have samples of Fisher Scientific..or maybe it's Richard-Allan's paraffin. It's called Histoplast PE. We use Leica's Paraplast Plus currently and I would like to know if anyone can share their preference and if you can offer any comments about the differences in the paraffin. Also, can I mix the two? Thanks so much in advance and have a great day. Paula Lab Manager Bio-Path Medical Group From Charles.Bacon at baystatehealth.org Tue Dec 22 12:30:08 2020 From: Charles.Bacon at baystatehealth.org (Bacon, Charles) Date: Tue, 22 Dec 2020 18:30:08 +0000 Subject: [Histonet] Paraffin Preference Message-ID: <92169d557e6c4805ac0a0857c31d0df5@ZXSWEXCHMXPR01.bhs.org> Hi Paula, I have worked in a couple different labs and in one lab we used Paraplast Plus. Because of the DMSO, tissue infiltration is superb with that paraffin. Unfortunately DMSO is not good for stainless steel so when we had to replace our processor (now with a stainless retort) we also had to replace our paraffin. We opted for Polyscientific Infiltrating/Embedding Paraffin Prills, Catalog Number: c827. It is a bit more money but after testing several other paraffins including the Richard Alan ones, this was the closest we could get to the performance of the Paraplast Plus. This is only the case if you are using one paraffin for BOTH infiltration/processing and embedding. If so, this paraffin is the best for the money. If you want to have two separate paraffins, one for processing only and a second type for embedding then your options are wide open. You can opt for paraffin where the chemistry has less plastics for processing and more for embedding, giving you a more supportive block. In the lab I am in now we also use the Polyscientific for both processing and embedding. Good luck, Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786? Fax: 413-322-4790 Charles.Bacon at baystatehealth.org -----Original Message----- From: Paula Sent: Tuesday, December 22, 2020 9:36 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Paraffin Preference Hi everyone..good morning. I have samples of Fisher Scientific..or maybe it's Richard-Allan's paraffin. It's called Histoplast PE. We use Leica's Paraplast Plus currently and I would like to know if anyone can share their preference and if you can offer any comments about the differences in the paraffin. Also, can I mix the two? Thanks so much in advance and have a great day. Paula Lab Manager Bio-Path Medical Group ---------------------------------------------------------------------- Please view our annual report at http://www.bhannualreport.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at 413-794-0000 and destroy all copies of this communication and any attachments. For further information regarding Baystate Health's privacy policy, please visit our Internet site at https://www.baystatehealth.org. From jmyers1 at aol.com Tue Dec 22 16:08:05 2020 From: jmyers1 at aol.com (Joe Myers) Date: Tue, 22 Dec 2020 17:08:05 -0500 Subject: [Histonet] Detection systems for IHC's References: Message-ID: Charles: Since the literature for BioGenex?s detection reagents for their Xmatrx Infinity system (XvisTM) indicates use of an ?enhancing? reagent prior to application of the (tertiary) antibody/polymer/enzyme reagent, such a detection system would be defined as a ?two-step? method. In contrast, Leica?s Refine reagent is a one-step reagent/method. Generally speaking, two-step methods are usually more sensitive than one-step methods, but since I?ve not conducted a comparison of the results obtained with these quite different reagents/methods, I can?t offer any advice on which is actually ?better?. I hope you find this feedback useful. Cheers, Joe Myers, M.S., CT/QIHC(ASCP) ************************************ Message: 1 Date: Mon, 21 Dec 2020 18:57:57 From: Charles Riley To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Detection systems for IHC's DOes anyone know what the comparison detection kit for the Xfinity Matrix from Biogenex would be in relation to the Bond Refine detection kits?