From rcharles at pa.gov Mon Aug 3 12:42:01 2020 From: rcharles at pa.gov (Charles, Roger) Date: Mon, 3 Aug 2020 17:42:01 +0000 Subject: [Histonet] Bovine Corona Virus antibody Message-ID: Hello, For all the Veterinary Diagnostic people. Does anybody use the BCV (BC 6-4) antibody from RTI LLC in Brocklings South Dakota? Also does anyone use the Z3A5 from Kansas State University? We have compared the two and are getting conflicting results. I do not know if the Z3A5 is even still available. Does anybody know this too. Thank you, Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us From acoscetti at gmail.com Tue Aug 4 10:20:43 2020 From: acoscetti at gmail.com (Amanda Coscetti) Date: Tue, 4 Aug 2020 11:20:43 -0400 Subject: [Histonet] Job opening Message-ID: <7DF03338-4487-41E2-83AD-87CC3115EFDD@gmail.com> McLeod Health in Florence, SC is looking for a histotech if anyone is interested or if you know someone who might be Interested. www.mcleodhealth.org Thanks! Amanda From amyleehisto779 at gmail.com Tue Aug 4 13:13:42 2020 From: amyleehisto779 at gmail.com (Amy Lee) Date: Tue, 4 Aug 2020 11:13:42 -0700 Subject: [Histonet] process formalin-fixed tissues from animals infected with a virus Message-ID: Hello, Does anyone know what we should know about process formalin fixed animal tissue that has been infected with virus? What certificate/requirement/qualification we should have? We have never done this before and need to make decision if we should do it or not. Thank you very much, Amy From jqb7 at cdc.gov Wed Aug 5 07:39:53 2020 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)) Date: Wed, 5 Aug 2020 12:39:53 +0000 Subject: [Histonet] Update info. on old topic? Message-ID: Good morning! I need to provide some information to my team about film vs. glass coverslipping. I have a lot of positive info. regarding film but are any cons? And I need to come up wit the pros of glass over film. Anyone with extended experience have some current information for me? Thanks very much, Jeanine Sanders, BS, HT(ASCP), QIHCCM(ASCP) Centers for Diseases Control and Prevention 1600 Clifton Road NE MS H18-SB Bldg. 18, Rm SB-114 Atlanta, GA 30329 404-639-3590 From paula at excaliburpathology.com Wed Aug 5 08:00:37 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Wed, 5 Aug 2020 13:00:37 +0000 (UTC) Subject: [Histonet] Update info. on old topic? In-Reply-To: References: Message-ID: <1398404991.167739.1596632437483@mail.yahoo.com> I am interested in this too, with my main question being the quality of digital images from film coverslipped slides. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Wednesday, August 5, 2020, 07:57:05 AM CDT, Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet wrote: Good morning! I need to provide some information to my team about film vs. glass coverslipping. I have a lot of positive info. regarding film but are any cons? And I need to come up wit the pros of glass over film. Anyone with extended experience have some current information for me? Thanks very much, Jeanine Sanders, BS, HT(ASCP), QIHCCM(ASCP) Centers for Diseases Control and Prevention 1600 Clifton Road NE MS H18-SB Bldg. 18, Rm SB-114 Atlanta, GA 30329 404-639-3590 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CWaitts at Centrastate.com Wed Aug 5 10:22:48 2020 From: CWaitts at Centrastate.com (Waitts, Celeste) Date: Wed, 5 Aug 2020 15:22:48 +0000 Subject: [Histonet] MMR IHC validaton Message-ID: <8c30fb230afd42f7a54c520887028bc2@exchnode1.local.centrastate.com> HI, WHAT is everyone's opinion on the best way to validate the Roche MMR IHC panel. Cost, time, ease? Any help? Thank you Celeste Waitts Histology Supervisor Centrastate Medical Center From Karen.Heckford at DignityHealth.org Wed Aug 5 10:34:28 2020 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Wed, 5 Aug 2020 15:34:28 +0000 Subject: [Histonet] Cytology Message-ID: <903be99abb344593a73079761d76299b@PHX-EXCH-013.chw.edu> Good Morning, I rarely do Special stains or IHC's on Cytology cytospins or thin prep type slides. Should these slides be charged, air dried or fixed in 95% alcohol before doing Special stains or IHC's on them? Is there a good book or some sort of publication on cytology procedures regarding the above mentioned. I have tried and look it up and it seems all over the place on what to do. Any help would be greatly appreciated, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you From Timothy.Morken at ucsf.edu Wed Aug 5 10:38:14 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 5 Aug 2020 15:38:14 +0000 Subject: [Histonet] Update info. on old topic? In-Reply-To: References: Message-ID: Hi Jeanine! We are using film coverslipping exclusively now and scanning all slides. We moved to film because it dries almost instantly and can be scanned right away. It has worked very well. The only issues, and not specific to film coverslipping, are that some slides with low contrast sometimes are out of focus. That is primarily IHC slides with low contrast counter stain and low contrast specific staining. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet Sent: Wednesday, August 05, 2020 5:40 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Update info. on old topic? Good morning! I need to provide some information to my team about film vs. glass coverslipping. I have a lot of positive info. regarding film but are any cons? And I need to come up wit the pros of glass over film. Anyone with extended experience have some current information for me? Thanks very much, Jeanine Sanders, BS, HT(ASCP), QIHCCM(ASCP) Centers for Diseases Control and Prevention 1600 Clifton Road NE MS H18-SB Bldg. 18, Rm SB-114 Atlanta, GA 30329 404-639-3590 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=rzbMGLf4Y17ghrmD701AEURtk3lAZ5QHWvK_mUBrhTs&s=NdAh9f9sPJR8Cc0amnB3m1WH4qmQ7DH1q5uQoy7e2k4&e= From relia1 at earthlink.net Wed Aug 5 11:14:21 2020 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 5 Aug 2020 12:14:21 -0400 Subject: [Histonet] Exciting New Histology Opportunities!! Message-ID: <000001d66b43$7b758ad0$7260a070$@earthlink.net> Hello Histonetters, I hope you are having a great week. I wanted to send a quick note to tell you about the positions that I am working on and am most excited about.? Why am I excited about these positions? Because each of these clients was asked if I had someone for you today would you be ready to interview and hire Every One of these clients said YES!!! Here are the Super Sizzlin Hot Opportunities! Leadership Opportunities: North Carolina ? Greensboro North Carolina - Concord Histology Tech, IHC and Mohs Tech Opportunities N. Carolina Charlotte N. Carolina Wilmington ? Mohs N. Carolina Wilmington Florida Sarasota area California - Modesto ? IHC California Modesto All of these positions are full time and permanent!! My clients offer excellent compensation, benefits and in most cases either relocation or a sign-on bonus. Histopeeps, If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I place someone you refer You will earn a referral fee. If you are interested in any of these opportunities CALL /TEXT MY CELL ASAP!!! At 407-353-5070. I can also be reached toll free at the office at 866-607-3542 or at relia1 at earthlink.net to set up a time to talk at your convenience! Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From relia1 at earthlink.net Wed Aug 5 11:19:28 2020 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 5 Aug 2020 12:19:28 -0400 Subject: [Histonet] Quick question about Florida licensure. Message-ID: <001701d66b44$323b8d00$96b2a700$@earthlink.net> Hi Histopeeps! I have a quick question about Florida licensure. Can someone who holds a MLT(ASCP) get licensed and work as a histotech in Florida? Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From Andrew.Dilts at coxhealth.com Wed Aug 5 12:39:49 2020 From: Andrew.Dilts at coxhealth.com (Dilts,Andrew) Date: Wed, 5 Aug 2020 17:39:49 +0000 Subject: [Histonet] MMR IHC validaton In-Reply-To: <8c30fb230afd42f7a54c520887028bc2@exchnode1.local.centrastate.com> References: <8c30fb230afd42f7a54c520887028bc2@exchnode1.local.centrastate.com> Message-ID: <190ec899b76e446ca737a52caf384244@coxhealth.com> You've likely been sending MMR out regularly for some time now. Look at your send out logs to find cases that you have known results for and validate against those. In our case, lost expression was rare enough on some of the markers that we had to write an exception and do less than the full validation, but there is an exception for that very reason. Your Roche Field Application Specialist may be able to advise you on a protocol to use as a starting point for validation. Good luck! Andrew Dilts HTL(ASCP) Histo Supervisor, Laboratory Services Phone: (417) 269-5021 Andrew.Dilts at coxhealth.com www.coxhealth.com -----Original Message----- From: Waitts, Celeste Sent: Wednesday, August 5, 2020 10:23 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] MMR IHC validaton HI, WHAT is everyone's opinion on the best way to validate the Roche MMR IHC panel. Cost, time, ease? Any help? Thank you Celeste Waitts Histology Supervisor Centrastate Medical Center CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intendedrecipient(s) and may contain confidential and privileged information protected by law. ?Any unauthorized review, use, disclosure or distribution is prohibited.?If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From annigyg at gmail.com Wed Aug 5 13:57:34 2020 From: annigyg at gmail.com (Anne van Binsbergen) Date: Wed, 5 Aug 2020 20:57:34 +0200 Subject: [Histonet] Film coverslip vs glass coverslip Message-ID: Hi all I have used Sakura film coverslippers in several different facilities since 1997. In the ?early days? there were some issues with slip detachment but those were smartly sorted out by the manufacturers. 1) Film coverslips dry really fast - no clumps of stuck together slides to soak for sometimes days to separate. 2) Film is easy to remove - 3 mins in Acetone and a few dips in ethanol and xylene and you are good to go. 3) Drop a slide with a glass slip and it shatters. Drop a slide with a film slip and the glass breaks but the film slip holds it all together preserving the section. 4) Eager pathologists in a hurry to view the section, cannot ?smear? the film slip off the slide. 5) No pesky glass shards to deal with. Over 40 years in the saddle, in my opinion it?s no contest. Film is the best. Annie in Africa (aka annieinarabia) Recently retired Artist and Artisan Sent from my iPhone From madeleinehuey at gmail.com Wed Aug 5 16:08:10 2020 From: madeleinehuey at gmail.com (Madeleine Huey) Date: Wed, 5 Aug 2020 14:08:10 -0700 Subject: [Histonet] Histonet Digest, Vol 201, Issue 3 In-Reply-To: References: Message-ID: Date: Wed, 5 Aug 2020 15:34:28 +0000 From: "Heckford, Karen - SMMC-SF" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Cytology Message-ID: <903be99abb344593a73079761d76299b at PHX-EXCH-013.chw.edu> Content-Type: text/plain; charset="us-ascii" Good Morning, I rarely do Special stains or IHC's on Cytology cytospins or thin prep type slides. Should these slides be charged, air dried or fixed in 95% alcohol before doing Special stains or IHC's on them? Is there a good book or some sort of publication on cytology procedures regarding the above mentioned. I have tried and look it up and it seems all over the place on what to do. Any help would be greatly appreciated. Hello Karen, Long time no see! I always used Charged slides, and prefixed Methanol for ~ 5 min @ RT (not 95% alcohol) before staining (SS & IHC). Hope this helps! *Madeleine Huey, B.S., HTL & QIHC (ASCP)* Supervisor, Pathology/Histology & IPOX (MV & LG) El Camino Hospital 2500 Grant Road (Rm GC-33) Mountain View, CA 94040 Phone: 650-940-7038 Fax: 650-988-8387 Email: madeleine_h at elcaminohospital.org On Wed, Aug 5, 2020 at 10:14 AM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. process formalin-fixed tissues from animals infected with a > virus (Amy Lee) > 2. Update info. on old topic? > (Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)) > 3. Re: Update info. on old topic? (Paula Keene Pierce) > 4. MMR IHC validaton (Waitts, Celeste) > 5. Cytology (Heckford, Karen - SMMC-SF) > 6. Re: Update info. on old topic? (Morken, Timothy) > 7. Exciting New Histology Opportunities!! (Pam Barker) > 8. Quick question about Florida licensure. (Pam Barker) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 4 Aug 2020 11:13:42 -0700 > From: Amy Lee > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] process formalin-fixed tissues from animals > infected with a virus > Message-ID: > OdXM+2Y9GnBC+40g at mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hello, > Does anyone know what we should know about process formalin fixed animal > tissue that has been infected with virus? What > certificate/requirement/qualification we should have? We have never done > this before and need to make decision if we should do it or not. > > Thank you very much, > > Amy > > > ------------------------------ > > Message: 2 > Date: Wed, 5 Aug 2020 12:39:53 +0000 > From: "Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)" > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: [Histonet] Update info. on old topic? > Message-ID: > < > SA9PR09MB5325CBDE76121B31C6309F23804B0 at SA9PR09MB5325.namprd09.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > Good morning! > > I need to provide some information to my team about film vs. glass > coverslipping. I have a lot of positive info. regarding film but are any > cons? And I need to come up wit the pros of glass over film. Anyone with > extended experience have some current information for me? > > Thanks very much, > > Jeanine Sanders, BS, HT(ASCP), QIHCCM(ASCP) > Centers for Diseases Control and Prevention > 1600 Clifton Road NE > MS H18-SB > Bldg. 18, Rm SB-114 > Atlanta, GA 30329 > 404-639-3590 > > > > > ------------------------------ > > Message: 3 > Date: Wed, 5 Aug 2020 13:00:37 +0000 (UTC) > From: Paula Keene Pierce > To: "'histonet at lists.utsouthwestern.edu'" > , "Sanders, Jeanine > (CDC/DDID/NCEZID/DHCPP)" > Subject: Re: [Histonet] Update info. on old topic? > Message-ID: <1398404991.167739.1596632437483 at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > I am interested in this too, with my main question being the quality of > digital images from film coverslipped slides. > Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 > N Blue Lake DriveNorman, OK 73069PH 405-759-3953 > http://www.excaliburpathology.com > > A sharp knife is nothing without a sharp eye. - Klingon Proverb > > On Wednesday, August 5, 2020, 07:57:05 AM CDT, Sanders, Jeanine > (CDC/DDID/NCEZID/DHCPP) via Histonet > wrote: > > Good morning! > > I need to provide some information to my team about film vs. glass > coverslipping. I have a lot of positive info. regarding film but are any > cons? And I need to come up wit the pros of glass over film. Anyone with > extended experience have some current information for me? > > Thanks very much, > > Jeanine Sanders, BS, HT(ASCP), QIHCCM(ASCP) > Centers for Diseases Control and Prevention > 1600 Clifton Road NE > MS H18-SB > Bldg. 18, Rm SB-114 > Atlanta, GA 30329 > 404-639-3590 > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 4 > Date: Wed, 5 Aug 2020 15:22:48 +0000 > From: "Waitts, Celeste" > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: [Histonet] MMR IHC validaton > Message-ID: > <8c30fb230afd42f7a54c520887028bc2 at exchnode1.local.centrastate.com> > Content-Type: text/plain; charset="us-ascii" > > HI, > WHAT is everyone's opinion on the best way to validate the Roche MMR IHC > panel. Cost, time, ease? > Any help? > Thank you > Celeste Waitts > Histology Supervisor > Centrastate Medical Center > > > > > ------------------------------ > > Message: 5 > Date: Wed, 5 Aug 2020 15:34:28 +0000 > From: "Heckford, Karen - SMMC-SF" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Cytology > Message-ID: <903be99abb344593a73079761d76299b at PHX-EXCH-013.chw.edu> > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > I rarely do Special stains or IHC's on Cytology cytospins or thin prep > type slides. Should these slides be charged, air dried or fixed in 95% > alcohol before doing Special stains or IHC's on them? > > Is there a good book or some sort of publication on cytology procedures > regarding the above mentioned. I have tried and look it up and it seems > all over the place on what to do. > > Any help would be greatly appreciated, > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford at dignityhealth.org > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the > intended recipient, you have received this document in error. Any further > review, dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please > notify us immediately by reply email. Thank you > > > > ------------------------------ > > Message: 6 > Date: Wed, 5 Aug 2020 15:38:14 +0000 > From: "Morken, Timothy" > To: "Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)" > Cc: Histonet > Subject: Re: [Histonet] Update info. on old topic? > Message-ID: > < > BYAPR05MB57039195DFE1E6C67B67B1F7E74B0 at BYAPR05MB5703.namprd05.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > Hi Jeanine! > > We are using film coverslipping exclusively now and scanning all slides. > We moved to film because it dries almost instantly and can be scanned right > away. It has worked very well. The only issues, and not specific to film > coverslipping, are that some slides with low contrast sometimes are out of > focus. That is primarily IHC slides with low contrast counter stain and low > contrast specific staining. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > -----Original Message----- > From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet < > histonet at lists.utsouthwestern.edu> > Sent: Wednesday, August 05, 2020 5:40 AM > To: 'histonet at lists.utsouthwestern.edu' > > Subject: [Histonet] Update info. on old topic? > > Good morning! > > I need to provide some information to my team about film vs. glass > coverslipping. I have a lot of positive info. regarding film but are any > cons? And I need to come up wit the pros of glass over film. Anyone with > extended experience have some current information for me? > > Thanks very much, > > Jeanine Sanders, BS, HT(ASCP), QIHCCM(ASCP) Centers for Diseases Control > and Prevention > 1600 Clifton Road NE > MS H18-SB > Bldg. 18, Rm SB-114 > Atlanta, GA 30329 > 404-639-3590 > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=rzbMGLf4Y17ghrmD701AEURtk3lAZ5QHWvK_mUBrhTs&s=NdAh9f9sPJR8Cc0amnB3m1WH4qmQ7DH1q5uQoy7e2k4&e= > > > > ------------------------------ > > Message: 7 > Date: Wed, 5 Aug 2020 12:14:21 -0400 > From: "Pam Barker" > To: "Histopeeps Histonet" > Subject: [Histonet] Exciting New Histology Opportunities!! > Message-ID: <000001d66b43$7b758ad0$7260a070$@earthlink.net> > Content-Type: text/plain; charset="iso-8859-1" > > Hello Histonetters, > I hope you are having a great week. > I wanted to send a quick note to tell you about the positions that I am > working on and am most excited about.? > Why am I excited about these positions? > Because each of these clients was asked if I had someone for you today > would > you be ready to interview and hire? > Every One of these clients said YES!!! > Here are the Super Sizzlin Hot Opportunities! > > Leadership Opportunities: > North Carolina ? Greensboro > North Carolina - Concord > > Histology Tech, IHC and Mohs Tech Opportunities > N. Carolina Charlotte > N. Carolina Wilmington ? Mohs > N. Carolina Wilmington > Florida Sarasota area > California - Modesto ? IHC > California Modesto > > All of these positions are full time and permanent!! > My clients offer excellent compensation, benefits and in most cases either > relocation or a sign-on bonus. > > > Histopeeps, If you think you or someone you know might be interested in any > of these opportunities or would like to talk about a job search in another > area, please contact me. > If I place someone you refer You will earn a referral fee. > > If you are interested in any of these opportunities > CALL /TEXT MY CELL ASAP!!! At 407-353-5070. > > > I can also be reached toll free at the office at 866-607-3542 or at > relia1 at earthlink.net to set up a time to talk at your convenience! > > Thanks-Pam > > Right Time, Right Place, Right Move with RELIA! > *15 Years!* > Celebrating 15 years of service exclusively to the Histology Community! > > Thank You! > ?Pam M. Barker? > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell:???? (407)353-5070 > FAX:???? (407)678-2788 > E-mail: relia1 at earthlink.net > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > www.facebook.com/PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > check out our latest opportunities at: > http://www.jobvertise.com/members/relia1 > #jobs4myhistopeeps > #ilovemyhistopeeps > #histopeeps > Follow my hashtags and make your day great and your career greater!! > > > > > > > ------------------------------ > > Message: 8 > Date: Wed, 5 Aug 2020 12:19:28 -0400 > From: "Pam Barker" > To: "Histopeeps Histonet" > Subject: [Histonet] Quick question about Florida licensure. > Message-ID: <001701d66b44$323b8d00$96b2a700$@earthlink.net> > Content-Type: text/plain; charset="us-ascii" > > Hi Histopeeps! > I have a quick question about Florida licensure. Can someone who holds a > MLT(ASCP) get licensed and work as a histotech in Florida? > > Thanks-Pam > > Right Time, Right Place, Right Move with RELIA! > *15 Years!* > Celebrating 15 years of service exclusively to the Histology Community! > > Thank You! > Pam M. Barker > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1 at earthlink.net > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > www.facebook.com/PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > check out our latest opportunities at: > http://www.jobvertise.com/members/relia1 > #jobs4myhistopeeps > #ilovemyhistopeeps > #histopeeps > Follow my hashtags and make your day great and your career greater!! > > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 201, Issue 3 > **************************************** > From edmartin26 at gmail.com Wed Aug 5 16:18:59 2020 From: edmartin26 at gmail.com (Eddie Martin) Date: Wed, 5 Aug 2020 17:18:59 -0400 Subject: [Histonet] Quick question about Florida licensure. In-Reply-To: <001701d66b44$323b8d00$96b2a700$@earthlink.net> References: <001701d66b44$323b8d00$96b2a700$@earthlink.net> Message-ID: <15C11400-920F-4D41-8233-41F1D1E5D3CA@gmail.com> Hi Pam, The quick response is that in Florida, an MLT with a MLT license cannot work as histotech unless they are working in Biomes, research, vending or in government. The Florida license specifies which type of clinical laboratories the technician/technologist is approved to work in. The Florida license states on it which laboratories the technician or technologist is approved to work in. If they request with the state to apply for state licensure in histology, then they must meet the requirements Prior to applying for licensure. V/r, Eddie Martin > On Aug 5, 2020, at 12:19 PM, Pam Barker wrote: > > ?Hi Histopeeps! > I have a quick question about Florida licensure. Can someone who holds a > MLT(ASCP) get licensed and work as a histotech in Florida? > > Thanks-Pam > > Right Time, Right Place, Right Move with RELIA! > *15 Years!* > Celebrating 15 years of service exclusively to the Histology Community! > > Thank You! > Pam M. Barker > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > E-mail: relia1 at earthlink.net > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > www.facebook.com/PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > check out our latest opportunities at: > http://www.jobvertise.com/members/relia1 > #jobs4myhistopeeps > #ilovemyhistopeeps > #histopeeps > Follow my hashtags and make your day great and your career greater!! > > > From SteveM at mcclainlab.com Wed Aug 5 22:41:24 2020 From: SteveM at mcclainlab.com (Steve McClain) Date: Thu, 6 Aug 2020 03:41:24 +0000 Subject: [Histonet] Histonet Digest, Vol 201, Issue 3 Update info. on old topic? In-Reply-To: References: Message-ID: Tim, Paula and Jeanine I have little experience w slide scanning, yet I have captured nearly 1.8M slide images, mostly on film coverslipped slides. One year ago we switched back to glass. 1) I suspect what Tim describes may be due to the fact that the refractive index of film differs from glass. We used film for nearly 20 years and for 4 years I could never get a sharp high power image. This was most noticeable when using 40x lens designed for glass cover slips. About 16 years ago, we found an Olympus 40x lens w a correction collar allowing for adjustment/ focusing suitable for coverslips of different thickness but useful also with either film or glass. The lens was expensive ($3600 if memory serves me). See https://www.edmundoptics.com/p/olympus-uplxapo-40x-objective-with-correction-collar/43026/ Perhaps the scanner manufacturer has a similar solution? 2) we were especially diligent (mono-maniacal) about using fresh reagents to keep water out of the last clearing reagent step-film has a nasty reputation for delaminating over time and pulling the sections off the glass. Several major academic centers have years and hundreds of thousands of slides now completely useless due to delamination. 3) film has problems with certain specimens routinely (bone and toenails and the thick cornified layer on volar skin) not cover slipping well leaving bubbles and ?cornflakes? or brown spots over the tissue. Cornflake artifact also occurs w water in the last reagent and may be seen on obscenely humid days. 4) film scratches readily when you stack slides or file them too tightly. Film Slides can be filed immediately after exam. Glass generally needs to wait 2 days before filing or one can glue up a brick of slides by filing too soon. 5) Film is faster and dries faster but glass is sharper and produces better images for my purposes (we switched back to glass 1 year ago- and the Leica model works perfectly well) 6) our old SCA film coverlipper generated more fumes than the new Leica. However all new instruments produce less fumes than older models. 7) glass coverslips are better stored in a desiccator/dry environment, before use. 8) This will sound stupid, yet not all coverslips of clean.- most (3/4) cheap coverslip glass is dirty and cannot be used in our lab where most/every slide is imaged. To determine this pick up an entire stack of coverslips and look through them. If they look clear-good to go; if they appear cloudy, find a new supplier. 9) glass coverslippers are finickyabout viscosity and volume of the mounting media. Once dialed in, we didn?t change media and use one brand and only one. 10) Not all film coverslip rolls are satisfactory either. We had the best luck w Brand S film and did not switch. Hope these observations help. Steve Steve A. McClain, MD 631-361-4000 Cell 631-926-3655 Good morning! I need to provide some information to my team about film vs. glass coverslipping. I have a lot of positive info. regarding film but are any cons? And I need to come up wit the pros of glass over film. Anyone with extended experience have some current information for me? Message: 3 Date: Wed, 5 Aug 2020 13:00:37 I am interested in this too, with my main question being the quality of digital images from film coverslipped slides. Paula Keene Pierce, BS, HTL ------------------------------ Message: 6 Date: Wed, 5 Aug 2020 15:38:14 +0000 From: "Morken, Timothy" To: "Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)" Cc: Histonet Subject: Re: [Histonet] Update info. on old topic? Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Jeanine! We are using film coverslipping exclusively now and scanning all slides. We moved to film because it dries almost instantly and can be scanned right away. It has worked very well. The only issues, and not specific to film coverslipping, are that some slides with low contrast sometimes are out of focus. That is primarily IHC slides with low contrast counter stain and low contrast specific staining. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet Sent: Wednesday, August 05, 2020 5:40 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Update info. on old topic? Good morning! I need to provide some information to my team about film vs. glass coverslipping. I have a lot of positive info. regarding film but are any cons? And I need to come up wit the pros of glass over film. Anyone with extended experience have some current information for me? Thanks very much, Jeanine Sanders, BS, From penny.marr at nhs.net Thu Aug 6 04:46:57 2020 From: penny.marr at nhs.net (MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST)) Date: Thu, 6 Aug 2020 09:46:57 +0000 Subject: [Histonet] Update info. on old topic? In-Reply-To: References: Message-ID: Hi Jeanine, - Film coverslips scratch very easily. Any dust in the system will scratch the film. - Staining is often compromised if the film coverslip needs to be removed. - Specific storage conditions are required to validate the adhesive on the film. If the storage conditions are not met the longevity of the tape is not guaranteed. - Glass is better for photography - Glass does not scratch as easily - Less fussy with storage requirements - Removal does not compromise staining quality. I hope this helps. Penny Marr Senior BMS C/- Histology Conquest Hospital St Leonards-on-Sea TN37 7RD penny.marr at nhs.net (01424) 758023 ext 734914 0300 133 4914 -----Original Message----- From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) [mailto:jqb7 at cdc.gov] Sent: 05 August 2020 13:40 To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Update info. on old topic? Good morning! I need to provide some information to my team about film vs. glass coverslipping. I have a lot of positive info. regarding film but are any cons? And I need to come up wit the pros of glass over film. Anyone with extended experience have some current information for me? Thanks very much, Jeanine Sanders, BS, HT(ASCP), QIHCCM(ASCP) Centers for Diseases Control and Prevention 1600 Clifton Road NE MS H18-SB Bldg. 18, Rm SB-114 Atlanta, GA 30329 404-639-3590 ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in relation to its contents. To do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland. NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and other accredited email services. For more information and to find out how you can switch, https://portal.nhs.net/help/joiningnhsmail From greg.dobbin at gmail.com Thu Aug 6 06:10:03 2020 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Thu, 6 Aug 2020 08:10:03 -0300 Subject: [Histonet] process formalin-fixed tissues from animals infected with a virus Message-ID: Hi Amy, Formalin fixed tissue is no longer infectious...unless you are talking about prions (eg scrapie, BSE, etc). So there should otherwise be no concerns or additional precautions required. Cheers, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From john.garratt at ciqc.ca Thu Aug 6 09:02:30 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 06 Aug 2020 14:02:30 +0000 Subject: [Histonet] process formalin-fixed tissues from animals infected with a virus In-Reply-To: References: Message-ID: Evaluation of Virus Inactivation by Formaldehyde to Enhance Biosafety of Diagnostic Electron Microscopy https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353909/ It is nice to have a reference. John On Thu, Aug 6, 2020 at 4:10 AM, Greg Dobbin via Histonet wrote: > Hi Amy, > Formalin fixed tissue is no longer infectious...unless you are talking > about prions (eg scrapie, BSE, etc). So there should otherwise be no > concerns or additional precautions required. > Cheers, > Greg > > -- > *Greg Dobbin* > 1205 Pleasant Grove Rd > RR#2 York, > PE C0A 1P0 > > *Everything in moderation...even moderation itself**!* > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From greg.dobbin at gmail.com Thu Aug 6 09:36:09 2020 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Thu, 6 Aug 2020 11:36:09 -0300 Subject: [Histonet] process formalin-fixed tissues from animals infected with a virus In-Reply-To: References: Message-ID: Very interesting paper John! Thank you. I wish the authors had also experimented with higher concentrations of formaldehyde (eg 10% formalin). Might one infer that 10% would be even more efficient in inactivating viral infectivity than 2 and 4%? ? Cheers Greg On Thu, Aug 6, 2020 at 11:02 AM John Garratt wrote: > Evaluation of Virus Inactivation by Formaldehyde to Enhance Biosafety of > Diagnostic Electron Microscopy > > > https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4353909/ > > > It is nice to have a reference. > > > > John > > On Thu, Aug 6, 2020 at 4:10 AM, Greg Dobbin via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > Hi Amy, > Formalin fixed tissue is no longer infectious...unless you are talking > about prions (eg scrapie, BSE, etc). So there should otherwise be no > concerns or additional precautions required. > Cheers, > Greg > > -- > *Greg Dobbin* > 1205 Pleasant Grove Rd > > RR#2 York, > PE C0A 1P0 > > > *Everything in moderation...even moderation itself**!* > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From jwwalker at rrmc.org Thu Aug 6 09:41:23 2020 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Thu, 6 Aug 2020 14:41:23 +0000 Subject: [Histonet] Film coverslip vs glass coverslip In-Reply-To: References: Message-ID: I respect your view point but as someone who has trained histotechs and cytotechs, the film leaves residual dots from dotting pens making it a challenge to test individual's ability to locate and identify tissues and cells of interest. That is the only draw back that I have seen in my years with using both film and glass. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology and Interim Phlebotomy Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Anne van Binsbergen via Histonet Sent: Wednesday, August 5, 2020 2:58 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Film coverslip vs glass coverslip [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. Hi all I have used Sakura film coverslippers in several different facilities since 1997. In the ?early days? there were some issues with slip detachment but those were smartly sorted out by the manufacturers. 1) Film coverslips dry really fast - no clumps of stuck together slides to soak for sometimes days to separate. 2) Film is easy to remove - 3 mins in Acetone and a few dips in ethanol and xylene and you are good to go. 3) Drop a slide with a glass slip and it shatters. Drop a slide with a film slip and the glass breaks but the film slip holds it all together preserving the section. 4) Eager pathologists in a hurry to view the section, cannot ?smear? the film slip off the slide. 5) No pesky glass shards to deal with. Over 40 years in the saddle, in my opinion it?s no contest. Film is the best. Annie in Africa (aka annieinarabia) Recently retired Artist and Artisan Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!I87qwjxLstg3H_X5!pM9H3mA1NYDmu-FOWGqiln3T8yEOTG-7DCR3HhhU_CTVGQIS0cMq7BBCPAdWEGY$ [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] From Timothy.Morken at ucsf.edu Thu Aug 6 10:37:19 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 6 Aug 2020 15:37:19 +0000 Subject: [Histonet] slide scanning and glass vs plastic RE: Histonet Digest, Vol 201, Message-ID: Steve, I agree with your whole thing. And our pathologist fought against plastic for years simply due to the fact it scratches easily and we are constantly pulling and filing slides for various needs. However, when we decided to go to scanning slides wet mounting media was never going to work - takes too long to dry and any gloppiness on the slide gums up the scanner. And no one is going to wait a day or more for their slides to be scanned when the purpose is to do remote diagnostics in real time. We started with frozens several years ago and worked out a lot of details (hand- applied). Then started scanning control slides in histology a couple years ago to allow remote sign off of those. This year we started slowly moving various types of specimens to plastic and scanning. Then when Covid hit and everyone had to work remotely we went in for scanning everything. Luckily we had enough scanning capacity to do that. So far it has worked well. For diagnostics the scanned slide is fine 99 or more percent of the time. Occasionally we get problem scans and a slide has to be viewed manually. Actually the biggest problem has been network bandwidth to review images from home - often too slow. Currently we are still sending the slides to the pathologists office, but most of the time they are not looked at and end up going to storage. We expect to move to simply scanning and storing and may never touch the slide again - the images can be used for all teaching, conferences, etc. So the scratch problem is solved in that way. Glass will probably always be better than plastic for photography, but the vast majority of diagnostic slides in practice are never photographed. We'll see within the year if various pathologists want recut with glass coverslipping expressly for photography for articles and books. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Steve McClain via Histonet Sent: Wednesday, August 05, 2020 8:41 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 201, Issue 3 Update info. on old topic? Tim, Paula and Jeanine I have little experience w slide scanning, yet I have captured nearly 1.8M slide images, mostly on film coverslipped slides. One year ago we switched back to glass. 1) I suspect what Tim describes may be due to the fact that the refractive index of film differs from glass. We used film for nearly 20 years and for 4 years I could never get a sharp high power image. This was most noticeable when using 40x lens designed for glass cover slips. About 16 years ago, we found an Olympus 40x lens w a correction collar allowing for adjustment/ focusing suitable for coverslips of different thickness but useful also with either film or glass. The lens was expensive ($3600 if memory serves me). See https://urldefense.proofpoint.com/v2/url?u=https-3A__www.edmundoptics.com_p_olympus-2Duplxapo-2D40x-2Dobjective-2Dwith-2Dcorrection-2Dcollar_43026_&d=DwIF-g&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=IZq7sglnD7BZh6tQuwuXoW8Xdke6Vv0QQ-hNSDBkFzk&s=B_LEPrG8dgAhhmRiptOVT5tukWNA5pNLY1iJ7AYW5do&e= Perhaps the scanner manufacturer has a similar solution? 2) we were especially diligent (mono-maniacal) about using fresh reagents to keep water out of the last clearing reagent step-film has a nasty reputation for delaminating over time and pulling the sections off the glass. Several major academic centers have years and hundreds of thousands of slides now completely useless due to delamination. 3) film has problems with certain specimens routinely (bone and toenails and the thick cornified layer on volar skin) not cover slipping well leaving bubbles and 'cornflakes' or brown spots over the tissue. Cornflake artifact also occurs w water in the last reagent and may be seen on obscenely humid days. 4) film scratches readily when you stack slides or file them too tightly. Film Slides can be filed immediately after exam. Glass generally needs to wait 2 days before filing or one can glue up a brick of slides by filing too soon. 5) Film is faster and dries faster but glass is sharper and produces better images for my purposes (we switched back to glass 1 year ago- and the Leica model works perfectly well) 6) our old SCA film coverlipper generated more fumes than the new Leica. However all new instruments produce less fumes than older models. 7) glass coverslips are better stored in a desiccator/dry environment, before use. 8) This will sound stupid, yet not all coverslips of clean.- most (3/4) cheap coverslip glass is dirty and cannot be used in our lab where most/every slide is imaged. To determine this pick up an entire stack of coverslips and look through them. If they look clear-good to go; if they appear cloudy, find a new supplier. 9) glass coverslippers are finickyabout viscosity and volume of the mounting media. Once dialed in, we didn't change media and use one brand and only one. 10) Not all film coverslip rolls are satisfactory either. We had the best luck w Brand S film and did not switch. Hope these observations help. Steve Steve A. McClain, MD 631-361-4000 Cell 631-926-3655 Good morning! I need to provide some information to my team about film vs. glass coverslipping. I have a lot of positive info. regarding film but are any cons? And I need to come up wit the pros of glass over film. Anyone with extended experience have some current information for me? Message: 3 Date: Wed, 5 Aug 2020 13:00:37 I am interested in this too, with my main question being the quality of digital images from film coverslipped slides. Paula Keene Pierce, BS, HTL ------------------------------ Message: 6 Date: Wed, 5 Aug 2020 15:38:14 +0000 From: "Morken, Timothy" To: "Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)" Cc: Histonet Subject: Re: [Histonet] Update info. on old topic? Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Jeanine! We are using film coverslipping exclusively now and scanning all slides. We moved to film because it dries almost instantly and can be scanned right away. It has worked very well. The only issues, and not specific to film coverslipping, are that some slides with low contrast sometimes are out of focus. That is primarily IHC slides with low contrast counter stain and low contrast specific staining. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet Sent: Wednesday, August 05, 2020 5:40 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Update info. on old topic? Good morning! I need to provide some information to my team about film vs. glass coverslipping. I have a lot of positive info. regarding film but are any cons? And I need to come up wit the pros of glass over film. Anyone with extended experience have some current information for me? Thanks very much, Jeanine Sanders, BS, _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIF-g&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=IZq7sglnD7BZh6tQuwuXoW8Xdke6Vv0QQ-hNSDBkFzk&s=pTaSonGU5ViUyGn6QIotezGywaH86AIMC_o7xiiSZ7Q&e= From kristyn.ferber at gmail.com Thu Aug 6 11:22:27 2020 From: kristyn.ferber at gmail.com (Kristyn Ferber) Date: Thu, 6 Aug 2020 16:22:27 +0000 Subject: [Histonet] Protocol for cutting frozen sections for RNA Sequencing Message-ID: Hello Histonet! Can anyone share their protocol for cutting frozen section (rolls) that will be used for RNA sequencing downstream much like this nature article: https://www.nature.com/articles/s41591-020-0844-1 The only direction we have been given is to cut the sections at 100 microns. Knowing RNA sequencing can be finicky, we want to ensure they have the best yield and we have a protocol that enables that. Best regards, Kristyn From jmyers1 at aol.com Thu Aug 6 15:33:40 2020 From: jmyers1 at aol.com (Joe Myers) Date: Thu, 6 Aug 2020 16:33:40 -0400 Subject: [Histonet] Cytology References: Message-ID: ? Ms. Heckford: As a cytotechnologist with just a little more than average experience with immunostaining procedures, I?d like to offer my input. To my knowledge, there are very few publications that address your concerns. I have, however, collected/prepared a significant amount of information on this topic, and I?m happy to share it with you. To address your specific questions: 1) When performing immunocytochemical (ICC) staining procedures, it?s always a good idea to use charged slides, since that will help with cellular retention; 2) specimen material should always be fixed, preferably in 95% ethanol (i.e. air-drying should be avoided); and 3) if you plan on routinely performing ICC, you?ll need to fully validate such procedures, particularly since antibodies that are characterized for IHC will not work in the same manner as they would in ICC. If you?d like, I?d be happy to share with you a PowerPoint presentation that I?ve given on several occasions relating to this topic. Cheers, Joe Myers, M.S., CT/QIHC(ASCP) - - - - - - - Date: Wed, 5 Aug 2020 15:34:28 +0000 From: "Heckford, Karen - SMMC-SF" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Cytology Good Morning, I rarely do Special stains or IHC's on Cytology cytospins or thin prep type slides. Should these slides be charged, air dried or fixed in 95% alcohol before doing Special stains or IHC's on them? Is there a good book or some sort of publication on cytology procedures regarding the above mentioned. I have tried and look it up and it seems all over the place on what to do. Any help would be greatly appreciated, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org From bethcoxx at gmail.com Thu Aug 6 19:20:30 2020 From: bethcoxx at gmail.com (Beth Cox) Date: Thu, 6 Aug 2020 20:20:30 -0400 Subject: [Histonet] Cytology (Special stains & IHC) Message-ID: <91266f12-91f2-8a7e-dfc1-5dbb36a80722@gmail.com> Hi Karen, The best resource for Cyto questions would probably be a recent edition of the Carson Histotechnology book.? The Fourth edition has some info on special stains for Cyto, but the new Fifth edition is expanded on that topic and has more info on IHCs for Cyto (Chapter 17). For _special stains_, you would definitely want them fixed in 95% alcohol.? The only exception to that would be fat stains which would of course require air-dried slides.? It would likely be advantageous to use charged slides, but that is not required. Remember when special staining Cyto slides fixed in 95% alcohol, that the deparaffinization steps at the beginning of the procedure must be skipped.? You are normally okay with using regular FFPE controls with your Cytospins or ThinPrep special stains. Doing _IHCs_ on Cytology preps (Cytospin or ThinPrep) gets a little trickier. _IF_ you are going to do them, you would want them to be 95% alcohol fixed and on charged slides.? However, you would be required to do a validation for Cytology for any/all antibodies you were going to run.? (Your CAP or CLIA inspector will eat you alive if you don't!).? Alcohol fixed specimens will require a new optimization since the pretreatments will be different than those for FFPE.?? Many antibodies, particularly nuclear ones, may be difficult or impossible to stain on the intact cells in a Cyto prep. Also, you would not be able to use FFPE controls for Cyto IHC since they are 'fixed and processed differently" than the Cyto slide and therefor don't properly control the process.? AND, alcohol fixed smears begin to lose their antigenicity in about 24 hours, so they must be stained the same day the slides are made, so having a control bank of alcohol fixed slides isn't feasible. Doing IHC on Cyto smears using FFPE protocols and FFPE controls is very likely to give you false negative results.? Unless you are doing large volume Cyto, it is very strongly recommended that you do your IHCs for Cyto on the FFPE cell block. Beth Cox, HTL/SCT(ASCP)QIHC ------------------------------ Message: 5 Date: Wed, 5 Aug 2020 15:34:28 +0000 From: "Heckford, Karen - SMMC-SF" To:"histonet at lists.utsouthwestern.edu" Subject: [Histonet] Cytology Message-ID:<903be99abb344593a73079761d76299b at PHX-EXCH-013.chw.edu> Content-Type: text/plain; charset="us-ascii" Good Morning, I rarely do Special stains or IHC's on Cytology cytospins or thin prep type slides. Should these slides be charged, air dried or fixed in 95% alcohol before doing Special stains or IHC's on them? Is there a good book or some sort of publication on cytology procedures regarding the above mentioned. I have tried and look it up and it seems all over the place on what to do. Any help would be greatly appreciated, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org From erin.mccarthy at tempus.com Mon Aug 10 14:34:17 2020 From: erin.mccarthy at tempus.com (Erin McCarthy) Date: Mon, 10 Aug 2020 14:34:17 -0500 Subject: [Histonet] Dako PD-L1 questions Message-ID: Hi all, I am looking to connect with people that stain PD-L1s on either of the Dako Autostainers. My company has been asked by a research/pharma partner if we would switch our IHC staining system to this brand. We currently use the Leica Bond IIIs. What I am curious about is how long a run takes, how reliable you find the instrumentation to be, and how user friendly it is. Additionally, does it require a lot of maintenance? Thank you all - feel free to DM me if you prefer. -- Erin McCarthy, HT (ASCP) Histology Supervisor Tempus Labs 600 W. Chicago Ave. Chicago IL 60654 Cell: (708)269-8610 -- This email and any attachments may contain confidential information. If you believe that you have received it in error, please delete the email and attachments and then notify Tempus by calling *800.739.4137*. From kdean70 at hotmail.com Tue Aug 11 09:34:38 2020 From: kdean70 at hotmail.com (Ken M) Date: Tue, 11 Aug 2020 14:34:38 +0000 Subject: [Histonet] Acid Fast Message-ID: Hi everyone- We have used a modified Ziehl Neelson stain on some breast tissue and found round shape organisms that are Acid Fast and not rod-like. Is anyone familiar with this before I send them to our Pathologist? Ken From john.garratt at ciqc.ca Tue Aug 11 09:42:35 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Tue, 11 Aug 2020 14:42:35 +0000 Subject: [Histonet] Acid Fast In-Reply-To: References: Message-ID: Hi Ken, have you checked all your reagents for contamination? John On Tue, Aug 11, 2020 at 7:34 AM, Ken M via Histonet wrote: > Hi everyone- We have used a modified Ziehl Neelson stain on some breast tissue and found round shape organisms that are Acid Fast and not rod-like. Is anyone familiar with this before I send them to our Pathologist? > > Ken > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From denise.long at uconn.edu Tue Aug 11 13:22:46 2020 From: denise.long at uconn.edu (Long, Denise) Date: Tue, 11 Aug 2020 18:22:46 +0000 Subject: [Histonet] Canine lymphatics antibody Message-ID: Does anyone in the veterinary or research sector know of an antibody that will label canine lymphatics? I would like information if you have any. Thanks in advance, Denise Denise M. Long, MS, HTL (ASCP), QIHC Histotechnologist/Immunohistochemist University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 From resultsachiever1 at hotmail.com Tue Aug 11 14:13:45 2020 From: resultsachiever1 at hotmail.com (resultsachiever1 at hotmail.com) Date: Tue, 11 Aug 2020 15:13:45 -0400 Subject: [Histonet] (no subject) Message-ID: From criley at dpspa.com Tue Aug 11 22:04:13 2020 From: criley at dpspa.com (Charles Riley) Date: Wed, 12 Aug 2020 03:04:13 +0000 Subject: [Histonet] End of shift Message-ID: 3 GBS inoculations started at 7:30 pm 1 started at 8:15 pm 42 blocks coming off at 5:51 am FNA prep kits filtered, refilled, and replaced on stock shelf Need EA-36 solution ASAP. Purchase order was approved but not sure who we purchase from. The thermo fischer supplier has a much higher price according to their website than what was approved. I believe the Statlab quoted price was also lower than what was approved through precoro. Can someone please make a decision and place the order tomorrow and then let me know what the plan should be going forward. From Valerie.Hannen at parrishmed.com Wed Aug 12 09:54:37 2020 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Wed, 12 Aug 2020 14:54:37 +0000 Subject: [Histonet] FREEZING CHAMBER Message-ID: Good morning all, I am hoping you can help me. We are looking for a "freezing chamber/bath" for freezing blocks for Frozen Sections. I have looked and can not find what I am looking for. What is everyone else using? We currently are using a "Thermoflask" with 2 Methlybutane and dry ice... this is not working so well...the chamber itself is too narrow. Any help would be greatly appreciated!! Thanks and have a great day!! Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com From M.McKinney at milestonemed.com Wed Aug 12 10:10:55 2020 From: M.McKinney at milestonemed.com (Mack McKinney) Date: Wed, 12 Aug 2020 15:10:55 +0000 Subject: [Histonet] FREEZING CHAMBER In-Reply-To: References: Message-ID: https://www.milestonemedsrl.com/product/prestochill/ Mack Mckinney Milestone Medical (469) 431-8670 ________________________________ From: Hannen, Valerie via Histonet Sent: Wednesday, August 12, 2020 9:54:37 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] FREEZING CHAMBER Good morning all, I am hoping you can help me. We are looking for a "freezing chamber/bath" for freezing blocks for Frozen Sections. I have looked and can not find what I am looking for. What is everyone else using? We currently are using a "Thermoflask" with 2 Methlybutane and dry ice... this is not working so well...the chamber itself is too narrow. Any help would be greatly appreciated!! Thanks and have a great day!! Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Donna.Willis at BSWHealth.org Wed Aug 12 10:16:45 2020 From: Donna.Willis at BSWHealth.org (Willis, Donna G) Date: Wed, 12 Aug 2020 15:16:45 +0000 Subject: [Histonet] FREEZING CHAMBER In-Reply-To: References: Message-ID: We use the PrestoChill from Milestone. Donna Willis Anatomic Pathology Manager Baylor University Medical Center Baylor Scott&White Health 214-820-2465 office ________________________________ From: Hannen, Valerie via Histonet Sent: Wednesday, August 12, 2020 9:54:37 AM To: Histonet at lists.utsouthwestern.edu Subject: {EXTERNAL} [Histonet] FREEZING CHAMBER CAUTION: This email originated outside of BSWH; avoid action unless you know the content is safe. Send suspicious emails as attachments to BadEmail at BSWHealth.org. Good morning all, I am hoping you can help me. We are looking for a "freezing chamber/bath" for freezing blocks for Frozen Sections. I have looked and can not find what I am looking for. What is everyone else using? We currently are using a "Thermoflask" with 2 Methlybutane and dry ice... this is not working so well...the chamber itself is too narrow. Any help would be greatly appreciated!! Thanks and have a great day!! Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com https://urldefense.com/v3/__http://www.parrishmed.com__;!!JA_k2roV-A!VyqWx6Inl3TzjNe3vEmfzx8d5mzfz7cwjrTQRqiFOmDEZpdoFT62I_ebfuXzHinutG5dCg$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!VyqWx6Inl3TzjNe3vEmfzx8d5mzfz7cwjrTQRqiFOmDEZpdoFT62I_ebfuXzHimqhCo6NA$ ********************************************************************** The information contained in this e-mail may be privileged and/or confidential, and protected from disclosure, and no waiver of any attorney-client, work product, or other privilege is intended. If you are the intended recipient, further disclosures are prohibited without proper authorization. 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From mwerdler at gmail.com Wed Aug 12 10:32:56 2020 From: mwerdler at gmail.com (Mca Werdler) Date: Wed, 12 Aug 2020 17:32:56 +0200 Subject: [Histonet] Automatization lab Message-ID: Hello everyone. I was wondering how your thoughts are about automating your lab (aka auto embedders and microtomes that are like a robots) what are your experiences? From relia1 at earthlink.net Wed Aug 12 11:00:19 2020 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 12 Aug 2020 12:00:19 -0400 Subject: [Histonet] Histology Leadership Opportunities in the Southeast! Can you help? Message-ID: <000001d670c1$ae113290$0a3397b0$@earthlink.net> Hello Histopeeps, How are you? I hope you are having a wonderful day. I have a couple of histology leadership positions and I need your help. I am currently working with clients in Alabama and North Carolina that are in need of exceptional histology professionals for several leadership roles. My question is do you know of anyone who might be interested in management positions in any of these areas? Or Histopeeps, Would you be interested in an opportunity in one of these areas? Incidentally, some of my clients are willing to consider lead techs ready to step up to supervisor! I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542, on my cell at 407-353-5070 or relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From criley at dpspa.com Wed Aug 12 16:39:38 2020 From: criley at dpspa.com (Charles Riley) Date: Wed, 12 Aug 2020 21:39:38 +0000 Subject: [Histonet] End of shift In-Reply-To: References: , Message-ID: I have been trying to order the stuff we need for cytoprep as I see we need it but I have never ordered anything for them before so its difficult for me to find everything and I don't have all the login information for our vendors so this is becoming very time consuming and drawing out the process. ________________________________ From: Alexis M. Hayslett Sent: Wednesday, August 12, 2020 8:30 AM To: Charles Riley ; Coordinators ; Pathologists ; histonet at lists.utsouthwestern.edu ; Debbie Hawkins Subject: Re: End of shift Need EA-36 solution ASAP. Purchase order was approved but not sure who we purchase from. The thermo fischer supplier has a much higher price according to their website than what was approved. I believe the Statlab quoted price was also lower than what was approved through precoro. Can someone please make a decision and place the order tomorrow and then let me know what the plan should be going forward- who is in charge of Cytology inventory/ordering? ________________________________ From: Charles Riley Sent: Tuesday, August 11, 2020 11:04 PM To: Coordinators ; Pathologists ; histonet at lists.utsouthwestern.edu ; Debbie Hawkins Subject: End of shift 3 GBS inoculations started at 7:30 pm 1 started at 8:15 pm 42 blocks coming off at 5:51 am FNA prep kits filtered, refilled, and replaced on stock shelf Need EA-36 solution ASAP. Purchase order was approved but not sure who we purchase from. The thermo fischer supplier has a much higher price according to their website than what was approved. I believe the Statlab quoted price was also lower than what was approved through precoro. Can someone please make a decision and place the order tomorrow and then let me know what the plan should be going forward. From Timothy.Morken at ucsf.edu Wed Aug 12 17:14:13 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 12 Aug 2020 22:14:13 +0000 Subject: [Histonet] Blank spots on IHC tissues - bubbles on Bond Message-ID: Has anyone solved a problem on the Leica Bond stainer of bubbles over tissue sections that cause unstained (ie, clear) areas where bubbles have formed? We've been trying things like extra buffer wash before reagent, new slide trays (tech rep suggestion). It is seen on single- antibody slides and very noticeable on dual - stain slides on which the first stain is there but the second stain shows numerous bubbles that do prevent the second stain from areas of the tissue. We can see the bubble artifact on the slide away from tissue sections as well. Any clues to solve this will be appreciated! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From Timothy.Morken at ucsf.edu Wed Aug 12 17:40:52 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 12 Aug 2020 22:40:52 +0000 Subject: [Histonet] Blank spots on IHC tissues - bubbles on Bond In-Reply-To: References: Message-ID: I'll add that we have tried new covertiles and this is on an old Bond Max. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Morken, Timothy via Histonet Sent: Wednesday, August 12, 2020 3:14 PM To: Histonet Subject: [Histonet] Blank spots on IHC tissues - bubbles on Bond Has anyone solved a problem on the Leica Bond stainer of bubbles over tissue sections that cause unstained (ie, clear) areas where bubbles have formed? We've been trying things like extra buffer wash before reagent, new slide trays (tech rep suggestion). It is seen on single- antibody slides and very noticeable on dual - stain slides on which the first stain is there but the second stain shows numerous bubbles that do prevent the second stain from areas of the tissue. We can see the bubble artifact on the slide away from tissue sections as well. Any clues to solve this will be appreciated! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=jXztO5Mou5-kWgu9wfRQ5awdMHezP6IUtwh8xGnmy08&s=ld7ZmhrPfWWBsLBEoNLhEsEgeaoscRokVUePMHUaBHw&e= From paula at excaliburpathology.com Wed Aug 12 17:42:06 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Wed, 12 Aug 2020 22:42:06 +0000 (UTC) Subject: [Histonet] Blank spots on IHC tissues - bubbles on Bond In-Reply-To: References: Message-ID: <1331962302.852304.1597272126938@mail.yahoo.com> I have seen this when using Sequenza cover plates.? My thoughts were that it is dissolved gases in the washes. As I noticed that when filling a container with my tap water and leaving it a while, bubbles form on the sides.? Do you brush away bubbles in your water bath even when using distilled water too? Try not using freshly made, stirred, agitated, etc. solutions and adding a surfactant.? Extreme solution: place solutions in a vacuum.? Sent from Smallbiz Yahoo Mail for iPhone On Wednesday, August 12, 2020, 5:25 PM, Morken, Timothy via Histonet wrote: Has anyone solved a problem on the Leica Bond stainer of bubbles over tissue sections that cause unstained (ie, clear) areas where bubbles have formed? We've been trying things like extra buffer wash before reagent, new slide trays (tech rep suggestion). It is seen on single- antibody slides and very noticeable on dual - stain slides on which the first stain is there but the second stain shows numerous bubbles that do prevent the second stain from areas of the tissue.? We can see the bubble artifact on the slide away from tissue sections as well. Any clues to solve this will be appreciated! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chak_bou at yahoo.com Wed Aug 12 18:30:17 2020 From: chak_bou at yahoo.com (Chakib Boussahmain) Date: Wed, 12 Aug 2020 23:30:17 +0000 (UTC) Subject: [Histonet] Protocol or SOP of how to make Tissue microarray References: <946308135.865654.1597275017679.ref@mail.yahoo.com> Message-ID: <946308135.865654.1597275017679@mail.yahoo.com> Hi Histonetters,Does anyone have a written SOP or a protocol of how to make Tissue microarray that can share with me. Any input will be greatly appreciated.Thank you in advanceChakib BoussahmainHistotechnologist From Joanna.Bartczak at uhn.ca Thu Aug 13 12:09:42 2020 From: Joanna.Bartczak at uhn.ca (Bartczak, Joanna) Date: Thu, 13 Aug 2020 17:09:42 +0000 Subject: [Histonet] Blank spots on IHC tissues - bubbles on Bond In-Reply-To: <1331962302.852304.1597272126938@mail.yahoo.com> References: , <1331962302.852304.1597272126938@mail.yahoo.com> Message-ID: I have seen this phenomenon years ago on the Bond III when I worked at a different laboratory. If I remember correctly, Leica had to replace the syringes and lines as air bubbles were being introduced when aspirating reagents. I would definitely contact Leica technical service and let them investigate. Good luck. Sincerely, Joanna Joanna Bartczak Charge Technologist - Immunopathology University Health Network - Toronto General Site 200 Elizabeth Street Toronto, Ontario M5G 2C4 Phone: (416) 340-4800 ext.6004 Joanna.Bartczak at uhn.ca ________________________________ From: Paula Keene Pierce Sent: Wednesday, August 12, 2020 6:42 PM To: Morken, Timothy ; Histonet Subject: Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond I have seen this when using Sequenza cover plates. My thoughts were that it is dissolved gases in the washes. As I noticed that when filling a container with my tap water and leaving it a while, bubbles form on the sides. Do you brush away bubbles in your water bath even when using distilled water too? Try not using freshly made, stirred, agitated, etc. solutions and adding a surfactant. Extreme solution: place solutions in a vacuum. Sent from Smallbiz Yahoo Mail for iPhone On Wednesday, August 12, 2020, 5:25 PM, Morken, Timothy via Histonet wrote: Has anyone solved a problem on the Leica Bond stainer of bubbles over tissue sections that cause unstained (ie, clear) areas where bubbles have formed? We've been trying things like extra buffer wash before reagent, new slide trays (tech rep suggestion). It is seen on single- antibody slides and very noticeable on dual - stain slides on which the first stain is there but the second stain shows numerous bubbles that do prevent the second stain from areas of the tissue. We can see the bubble artifact on the slide away from tissue sections as well. Any clues to solve this will be appreciated! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!CjcC7IQ!eYy1vmeWP5MP1WLTAt5O-QHHI3LnxW8O7vVLgSKZM005u_kiBtXjm1VAhKCf_Vw3cecE$ This e-mail may contain confidential and/or privileged information for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. Opinions, conclusions or other information contained in this e-mail may not be that of the organization. If you feel you have received an email from UHN of a commercial nature and would like to be removed from the sender's mailing list please do one of the following: (1) Follow any unsubscribe process the sender has included in their email (2) Where no unsubscribe process has been included, reply to the sender and type "unsubscribe" in the subject line. If you require additional information please go to our UHN Newsletters and Mailing Lists page. Please note that we are unable to automatically unsubscribe individuals from all UHN mailing lists. From Timothy.Morken at ucsf.edu Thu Aug 13 12:16:51 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 13 Aug 2020 17:16:51 +0000 Subject: [Histonet] Blank spots on IHC tissues - bubbles on Bond In-Reply-To: References: , <1331962302.852304.1597272126938@mail.yahoo.com> Message-ID: Joanna, thanks! You are the first who said they had seen it before. I have a Leica tech coming in tomorrow to check out the syringes and lines. That was my thought too. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From: Bartczak, Joanna Sent: Thursday, August 13, 2020 10:10 AM To: Paula Keene Pierce ; Morken, Timothy ; Histonet Subject: Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond I have seen this phenomenon years ago on the Bond III when I worked at a different laboratory. If I remember correctly, Leica had to replace the syringes and lines as air bubbles were being introduced when aspirating reagents. I would definitely contact Leica technical service and let them investigate. Good luck. Sincerely, Joanna Joanna Bartczak Charge Technologist - Immunopathology University Health Network - Toronto General Site 200 Elizabeth Street Toronto, Ontario M5G 2C4 Phone: (416) 340-4800 ext.6004 Joanna.Bartczak at uhn.ca ________________________________ From: Paula Keene Pierce > Sent: Wednesday, August 12, 2020 6:42 PM To: Morken, Timothy >; Histonet > Subject: Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond I have seen this when using Sequenza cover plates. My thoughts were that it is dissolved gases in the washes. As I noticed that when filling a container with my tap water and leaving it a while, bubbles form on the sides. Do you brush away bubbles in your water bath even when using distilled water too? Try not using freshly made, stirred, agitated, etc. solutions and adding a surfactant. Extreme solution: place solutions in a vacuum. Sent from Smallbiz Yahoo Mail for iPhone On Wednesday, August 12, 2020, 5:25 PM, Morken, Timothy via Histonet > wrote: Has anyone solved a problem on the Leica Bond stainer of bubbles over tissue sections that cause unstained (ie, clear) areas where bubbles have formed? We've been trying things like extra buffer wash before reagent, new slide trays (tech rep suggestion). It is seen on single- antibody slides and very noticeable on dual - stain slides on which the first stain is there but the second stain shows numerous bubbles that do prevent the second stain from areas of the tissue. We can see the bubble artifact on the slide away from tissue sections as well. Any clues to solve this will be appreciated! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!CjcC7IQ!eYy1vmeWP5MP1WLTAt5O-QHHI3LnxW8O7vVLgSKZM005u_kiBtXjm1VAhKCf_Vw3cecE$ This e-mail may contain confidential and/or privileged information for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. Opinions, conclusions or other information contained in this e-mail may not be that of the organization. If you feel you have received an email from UHN of a commercial nature and would like to be removed from the sender's mailing list please do one of the following: (1) Follow any unsubscribe process the sender has included in their email (2) Where no unsubscribe process has been included, reply to the sender and type "unsubscribe" in the subject line. If you require additional information please go to our UHN Newsletters and Mailing Lists page. Please note that we are unable to automatically unsubscribe individuals from all UHN mailing lists. From greg.dobbin at gmail.com Thu Aug 13 12:20:11 2020 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Thu, 13 Aug 2020 14:20:11 -0300 Subject: [Histonet] Blank Spots on IHC- Bubbles on Bond Message-ID: Hi Tim, The usual source of bubbles is a deteriorating syringe or lines or valve. Have you observed the syringe while running the Clean Fluidics maintenance function? There can be some "micro" bubbles in the syringe on first draw of a reagent however, if working properly these tiny bubbles quickly disappear in subsequent draws of that reagent. If bubbles persist in the barrel of the syringe throughout the flushing, then you have found the source of the problem. If the bubbles occur with every reagent then the syringe is the problem but if you only see bubbles in one of the reagents then it is a defective valve or line for that reagent. I hope this was helpful. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From edmartin26 at gmail.com Thu Aug 13 12:50:31 2020 From: edmartin26 at gmail.com (Eddie Martin) Date: Thu, 13 Aug 2020 13:50:31 -0400 Subject: [Histonet] Bubbles noticed on Bond-Max Message-ID: Hi Tim, From a user perspective: The slides you?re using may be a bad lot causing hydrophobic staining or you?re not cleaning your covertiles correctly. The wells on the assembly might also not be pulling adequately. Have you cleaned the assembly recently, particularly the wells? You could use a clean kit offer by Novocastra or make your own. Performing a decontamination on your instrument would be helpful too to include your bulk reagent compartments and reagent syringe. Have you also been performing the suggested manufacturer?s guidance on maintaining your Bond-Max IHC/ISH analyzer? Or... Simply the instrument needs to be recalibrated by a Leica engineer. Do not PM with a third party biomed group as the Leica engineer will need to recalibrate the instrument with a PM kit to check and if necessary replace the physical components, electronics, and fluidic system of your analyzer along with updating the coordinate system to match software build according to their latest recommendation from their home office. Apart from typical lab reagents or processes only performed by an engineer, do you have electrical or magnetic surges in your lab? The backup battery that comes with your Bond is typically good for two years, and would need to be replaced every few years, especially as the Bond-Max requires a step down from the 120V outlet to 110V. And this consideration can also deeply impact your overall staining. The application specialist should have more politely said that they didn?t know but will try to get a response back to you. I don?t comprehend how the tray would be the issue unless it was severely cracked and would prevent the slide assembly from functioning properly. Very respectfully, Eddie Martin, HT, HTL, QIHC Hematology Team Leader The National Institutes of Health 10 Center Drive Bethesda, MD 20892 Sent from my iPhone > On Aug 13, 2020, at 1:00 PM, histonet-request at lists.utsouthwestern.edu wrote: > > ?Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Re: End of shift (Charles Riley) > 2. Blank spots on IHC tissues - bubbles on Bond (Morken, Timothy) > 3. Re: Blank spots on IHC tissues - bubbles on Bond (Morken, Timothy) > 4. Re: Blank spots on IHC tissues - bubbles on Bond > (Paula Keene Pierce) > 5. Protocol or SOP of how to make Tissue microarray > (Chakib Boussahmain) > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amurvosh at advancederm.net Thu Aug 13 12:58:40 2020 From: amurvosh at advancederm.net (Anne Murvosh) Date: Thu, 13 Aug 2020 17:58:40 +0000 Subject: [Histonet] Mart-1 rapid test for Mohs specimens Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7CC3E57@Exchange.Advancederm.net> I'm working up a price comparison for NovoDiax's rapid 10 minute Mart- 1 stain for melanoma on Mohs specimens. The doctors would like me to price shop but I only know of 40 minute stains which is too long. Does anyone know of any other companies running a rapid 10-15 minute Mart- 1 stain? Thanks Anne Murvosh (HT) From Timothy.Morken at ucsf.edu Fri Aug 14 10:42:55 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 14 Aug 2020 15:42:55 +0000 Subject: [Histonet] Blank Spots on IHC- Bubbles on Bond In-Reply-To: References: Message-ID: Greg, thanks! I have a leica engineer coming in to day to look at it, specifically the fluidics system, so hopefully he?ll see what you mention and fix it. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From: Greg Dobbin Sent: Thursday, August 13, 2020 10:20 AM To: Morken, Timothy ; histonet at lists.utsouthwestern.edu Subject: RE: Blank Spots on IHC- Bubbles on Bond Hi Tim, The usual source of bubbles is a deteriorating syringe or lines or valve. Have you observed the syringe while running the Clean Fluidics maintenance function? There can be some "micro" bubbles in the syringe on first draw of a reagent however, if working properly these tiny bubbles quickly disappear in subsequent draws of that reagent. If bubbles persist in the barrel of the syringe throughout the flushing, then you have found the source of the problem. If the bubbles occur with every reagent then the syringe is the problem but if you only see bubbles in one of the reagents then it is a defective valve or line for that reagent. I hope this was helpful. Greg -- Greg Dobbin 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 Everything in moderation...even moderation itself! From sara.a.wells210 at gmail.com Sat Aug 15 09:02:28 2020 From: sara.a.wells210 at gmail.com (Sara Wells) Date: Sat, 15 Aug 2020 09:02:28 -0500 Subject: [Histonet] HTL exam 2020 best study guides and methods Message-ID: Hello, I am studying to take my HTL and I was wondering what recent HTL's used to study for the 2019-2020 exam? Thanks! Sara Wells From cindy38017 at yahoo.com Sat Aug 15 10:00:34 2020 From: cindy38017 at yahoo.com (cindy dewar) Date: Sat, 15 Aug 2020 15:00:34 +0000 (UTC) Subject: [Histonet] Method accuracy verification for CLIA References: <304315077.2462269.1597503634554.ref@mail.yahoo.com> Message-ID: <304315077.2462269.1597503634554@mail.yahoo.com> Hello...does anyone know how often CLIA requires the MAV be performed? Annually? Biannually? Departmental preference? Thanks so much! Cindy Sent from Yahoo Mail for iPad From Samantha.Golden at HCAHealthcare.com Sun Aug 16 10:32:30 2020 From: Samantha.Golden at HCAHealthcare.com (Samantha.Golden at HCAHealthcare.com) Date: Sun, 16 Aug 2020 15:32:30 +0000 Subject: [Histonet] CAP inspection Message-ID: I?m excited to participate in my first CAP audit as part of the inspection team, and I would love some tips and advice for a first timer. I don?t want to get stuck in the weeds as to what I?m searching for. While I?ve read all the info from CAP regarding a successful inspection, I?d love some first hand experience from those who have done this. Thanks a bunch. Samantha Golden, BS, HT(ASCP)cmQIHCcm --- Sent from Workspace ONE Boxer From patpxs at gmail.com Sun Aug 16 10:57:14 2020 From: patpxs at gmail.com (P Sicurello) Date: Sun, 16 Aug 2020 08:57:14 -0700 Subject: [Histonet] CAP inspection In-Reply-To: References: Message-ID: Hi Samantha, Have "fun" with your first inspection. A few pointers: try not to compare the place you are inspecting with your lab, try to stay focused on the task at hand (many new inspectors are easily influenced), walk around and get a feel for the lab- sure they are going to be tense because of the CAP inspection - but look around - does anything feel off?, SDS's are a good thing to check - many places don't update theirs and the SDS is either not current or comes from a different vendor than the chemical in use. You'll probably get lots of suggestions. Stay safe. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Sun, Aug 16, 2020 at 8:32 AM Samantha Golden, HT(ASCP) via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I?m excited to participate in my first CAP audit as part of the inspection > team, and I would love some tips and advice for a first timer. > > I don?t want to get stuck in the weeds as to what I?m searching for. While > I?ve read all the info from CAP regarding a successful inspection, I?d love > some first hand experience from those who have done this. > > Thanks a bunch. > Samantha Golden, BS, HT(ASCP)cmQIHCcm > > > --- > Sent from Workspace ONE Boxer > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From LRaff at uropartners.com Wed Aug 19 09:50:37 2020 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 19 Aug 2020 14:50:37 +0000 Subject: [Histonet] It makes what we do in the lab feel worthwhile--blog post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF17485527@COLOEXCH01.uropartners.local> Hope all our well. http://www.chicagonow.com/downsize-maybe/2020/08/this-makes-my-day/ Lester J. Raff, MD MBA FCAP Laboratory Director UroPartners LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 Telephone 708-486-0076 Fax 708-492-0203 From mashalsmom at gmail.com Wed Aug 19 12:11:00 2020 From: mashalsmom at gmail.com (Emily Horst) Date: Wed, 19 Aug 2020 13:11:00 -0400 Subject: [Histonet] Sausage controls Message-ID: Hello all, For those of you who use a sausage control for IHC/special stains, could you direct me as to process for starting that instead of using individual controls for everything? Thanks in advance! Emily From jmacdonald at mtsac.edu Wed Aug 19 12:29:52 2020 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Wed, 19 Aug 2020 17:29:52 +0000 Subject: [Histonet] Sausage controls In-Reply-To: References: Message-ID: The Journal of Histotechnology had an article regarding this years ago. Perhaps you can find it in the archives. Dr. Hector Battifora was the author. Jennifer -----Original Message----- From: Emily Horst via Histonet Sent: Wednesday, August 19, 2020 10:11 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Sausage controls EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Hello all, For those of you who use a sausage control for IHC/special stains, could you direct me as to process for starting that instead of using individual controls for everything? Thanks in advance! Emily _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mashalsmom at gmail.com Wed Aug 19 12:33:28 2020 From: mashalsmom at gmail.com (Emily Horst) Date: Wed, 19 Aug 2020 13:33:28 -0400 Subject: [Histonet] Sausage controls In-Reply-To: References: Message-ID: Thanks! On Wed, Aug 19, 2020 at 1:29 PM Mac Donald, Jennifer wrote: > The Journal of Histotechnology had an article regarding this years ago. > Perhaps you can find it in the archives. Dr. Hector Battifora was the > author. > > Jennifer > > > > -----Original Message----- > > From: Emily Horst via Histonet > > Sent: Wednesday, August 19, 2020 10:11 AM > > To: histonet at lists.utsouthwestern.edu > > Subject: [Histonet] Sausage controls > > > > EXTERNAL SENDER- Exercise caution with requests, links, and attachments. > > > > Hello all, > > > > For those of you who use a sausage control for IHC/special stains, could > you direct me as to process for starting that instead of using individual > controls for everything? > > > > Thanks in advance! > > > > Emily > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From dlh3878 at gmail.com Wed Aug 19 12:49:42 2020 From: dlh3878 at gmail.com (Donielle Hunter) Date: Wed, 19 Aug 2020 13:49:42 -0400 Subject: [Histonet] HSV control slides Message-ID: Good afternoon all. My lab is in desperate need of HSV I and II control slides. Our pathologist prefers two different tissue slides to compare. If anyone has any control blocks they would be willing to share I would happy to see if there is anything my lab has that can help you. Thanks in advance, Donielle Hunter From nelsonrnch at verizon.net Wed Aug 19 18:29:44 2020 From: nelsonrnch at verizon.net (Patti Nelson) Date: Wed, 19 Aug 2020 16:29:44 -0700 Subject: [Histonet] Humidity in the lab References: <5B0B2155-A99B-40B9-B007-704F8977065E.ref@verizon.net> Message-ID: <5B0B2155-A99B-40B9-B007-704F8977065E@verizon.net> Hello Histo peeps I would like to know what every uses to help with the humidity problem in the lab. Thank you in advance. Patti Nelson HT (ASCP) 909-841-9761 Sent from my iPhone From cforster at umn.edu Wed Aug 19 18:49:53 2020 From: cforster at umn.edu (Colleen Forster) Date: Wed, 19 Aug 2020 18:49:53 -0500 Subject: [Histonet] Humidity in the lab In-Reply-To: <5B0B2155-A99B-40B9-B007-704F8977065E@verizon.net> References: <5B0B2155-A99B-40B9-B007-704F8977065E.ref@verizon.net> <5B0B2155-A99B-40B9-B007-704F8977065E@verizon.net> Message-ID: Totally following this one... And what do you use to record humidity and what would be the cut off for good staining , especially for IHC stains. Colleen Forster On Wed, Aug 19, 2020 at 6:30 PM Patti Nelson via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello Histo peeps > > I would like to know what every uses to help with the humidity problem in > the lab. Thank you in advance. > > > Patti Nelson HT (ASCP) > 909-841-9761 > Sent from my iPhone > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 612-626-1930 From relia1 at earthlink.net Thu Aug 20 12:03:53 2020 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 20 Aug 2020 13:03:53 -0400 Subject: [Histonet] Summer is almost over!! Exciting opportunities Coast to Coast and Entry Level to Leadership! Message-ID: <000001d67713$e2411b90$a6c352b0$@earthlink.net> Hello Histopeeps, It is hard to believe the summer is almost over. The kids are going back to school and Labor Day is just a few days away! In a few more weeks -Halloween; Then Thanksgiving, Christmas and New Year's Day!! Looking for a new job in the Fall? Planning a job change after the holidays? Considering making a move in 2021? Let's Get The Ball Rolling!!! Histopeeps! **Are you a histo tech looking for a something better? **Are you a new or recent graduate of a histology school? **Are you a traveler considering switch into a permanent position? **Are you a Histotech looking to advance into leadership? **Are you a Supervisor looking to advance to lab management? Let's Get The Ball Rolling!!! All you have to do is contact me at relia1 at earthlink.net or call me at 866-607-3542 my office is open M-F 7am-8pm EST or by appointment. If you know of anyone else who might be interested in subscribing to RELIA's Histology Careers Bulletin please feel free to pass this along to them. Here is some information about my current openings: Histology Managers/Supervisors needed in: N. Carolina Histology Lab Supervisor - A RELIA Exclusive!! N. Carolina Histology Lab Manager Alabama Histology Lab Manager Histotechnicians/Histotechnologists needed in: N. Carolina IHC Specialist N. Carolina Mohs Tech California IHC Specialist California Histology Tech Florida Histotechnologist (Bradenton New Grads Welcome!!) New York Histotech 1st Shift! New Grads welcome! And new opportunities coming in EVERY DAY!! Remember it never hurts to look!!! Thanks, Pam 877-60 RELIA (877-607-3542) Cell/Text (407)353-5070 I know there are a lot of recruiters out there right now contacting you and your friends. RELIA is the only nationwide recruiting firm specializing in the permanent placement of histology professionals. Remember here at RELIA we work as your confidential advocate to help you make the move that is right for you when the time is right for you. Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From Christopher.Sheeder at seattlechildrens.org Thu Aug 20 12:12:45 2020 From: Christopher.Sheeder at seattlechildrens.org (Sheeder, Christopher) Date: Thu, 20 Aug 2020 17:12:45 +0000 Subject: [Histonet] Sausage controls In-Reply-To: References: Message-ID: <8be726b9bc4b4493a252c06588adf38b@seattlechildrens.org> Here is the article... Multitumor "Sausage" Blocks in Immunohistochemistry Simplified Method of Preparation, Practical Uses, and Roles in Quality Assurance Rodney T. Miller, M.D., Carol L. Groothuis, MT(ASCP)SI American Journal of Clinical Pathology, Volume 96, Issue 2, 1 August 1991, Pages 228-232, https://doi.org/10.1093/ajcp/96.2.228 Christopher Sheeder, HT(ASCP)CMQIHC Histology Supervisor | Department of Laboratories Seattle Children's Hospital 4800 Sand Point Way NE Seattle, WA 98105 Office: 206-987-6259 christopher.sheeder at seattlechildrens.org Seattle Children's - Internal -----Original Message----- From: Mac Donald, Jennifer Sent: Wednesday, August 19, 2020 10:30 AM To: Emily Horst Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Sausage controls The Journal of Histotechnology had an article regarding this years ago. Perhaps you can find it in the archives. Dr. Hector Battifora was the author. Jennifer -----Original Message----- From: Emily Horst via Histonet Sent: Wednesday, August 19, 2020 10:11 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Sausage controls EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Hello all, For those of you who use a sausage control for IHC/special stains, could you direct me as to process for starting that instead of using individual controls for everything? Thanks in advance! Emily _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIFAg&c=aBkXpkKi7gN5fe5MqrMaN-VmRugaRb1IDRfSv2xVRy0&r=c3bFZxewNEDI2wFyTyj_1jhuE1dV-f-i0gIzQyuiCw3aEzfs6mQm_0c0YpHyN0fx&m=fj4b9tOdVR0tBqtwawhTNGqy3ydx6AW8v-d9QPB0Af0&s=z_CRxXgU9gwTuxcX35Zf46MFZMgU7xAEwyXYef2jqGE&e= CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From modz9636 at gmail.com Fri Aug 21 10:37:31 2020 From: modz9636 at gmail.com (M.O.) Date: Fri, 21 Aug 2020 08:37:31 -0700 Subject: [Histonet] human shoulder joint fixation Message-ID: Happy Friday, Histonetters! I am doing some planning for a new project and wanted to get opinions on fixation of large pieces of tissue. We will have human shoulders, where we want to preserve the rotator cuff/joint. Cutting the tissue with a saw will damage the soft tissue, so we were thinking that post-fixation would be best for cutting slabs. Does anyone have experience with fixing such large pieces of tissue? We typically use zinc buffered formalin for fixation. Would a vacuum work or a vacuum sealer? Thank you for any input you might have!!!! Sincerely, Merissa From plucas at biopath.org Fri Aug 21 13:18:11 2020 From: plucas at biopath.org (Paula) Date: Fri, 21 Aug 2020 11:18:11 -0700 Subject: [Histonet] Leica Bond, ASP6025 Processor parts Message-ID: <002a01d677e7$6f0fde70$4d2f9b50$@biopath.org> Hi all, Does anyone know of a Biomed company that makes and/or sells parts for the Bond III and the ASP6025 processor? It's amazing how much Leica wants for their parts. For example, the waste bottle used to collect the buffer waste is $674 and the seal for the retort's lid is $597. Hope someone can help..thanks in advance. Paula Bio-Path Medical Group Fountain Valley, CA From Daniel.Pesino at TrinityHealthOfNE.org Fri Aug 21 13:43:46 2020 From: Daniel.Pesino at TrinityHealthOfNE.org (Daniel Pesino) Date: Fri, 21 Aug 2020 18:43:46 +0000 Subject: [Histonet] [External] Leica Bond, ASP6025 Processor parts In-Reply-To: <002a01d677e7$6f0fde70$4d2f9b50$@biopath.org> References: <002a01d677e7$6f0fde70$4d2f9b50$@biopath.org> Message-ID: You should see how much they want for a wax drain hose for the Peloris processors... A tube and a nozzle for +$1,000. I ended up building my own with parts from Home Depot for about $30. We use Perkins Biomedical for our preventative maintenance and parts. They do repairs throughout the northeast, but should be able to send you parts. They may have a spare retort lid that they can quote you. You will probably need someone else to install it in California. You can check them out here: https://perkinsbiomed.com/ Or you can reach them at 800-975-7456. They are super professional and knowledgeable. Daniel Pesino, HTL(ASCP)CMQIHCCM Senior Histotechnologist Trinity Health Of New England Daniel.Pesino at TrinityHealthOfNE.org W? 860-714-4675 114 Woodland Street Hartford, CT 06105 TrinityHealthOfNE.org | Facebook | Twitter | Instagram -----Original Message----- From: Paula via Histonet Sent: Friday, August 21, 2020 2:18 PM To: histonet at lists.utsouthwestern.edu Subject: [External] [Histonet] Leica Bond, ASP6025 Processor parts Warning: This email originated from the Internet! DO NOT CLICK links if the sender is unknown, and NEVER provide your password. Hi all, Does anyone know of a Biomed company that makes and/or sells parts for the Bond III and the ASP6025 processor? It's amazing how much Leica wants for their parts. For example, the waste bottle used to collect the buffer waste is $674 and the seal for the retort's lid is $597. Hope someone can help..thanks in advance. Paula Bio-Path Medical Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From rsrichmond at gmail.com Fri Aug 21 17:25:25 2020 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 21 Aug 2020 18:25:25 -0400 Subject: [Histonet] human shoulder joint fixation In-Reply-To: References: Message-ID: > > Merissa (where?) asks; >>I am doing some planning for a new project and > wanted to get opinions on fixation of large pieces of tissue. We will have > human shoulders, where we want to preserve the rotator cuff/joint. Cutting > the tissue with a saw will damage the soft tissue, so we were thinking that > post-fixation would be best for cutting slabs. - Does anyone have > experience with fixing such large pieces of tissue? We typically use zinc > buffered formalin for fixation. Would a vacuum work or a vacuum sealer?<< > This is a non-trivial problem, and I hope you're working closely with the pathologist or other investigator who's going to be looking at the slides. Formalin (forget the zinc) penetrates tissue slowly enough that you're not going to get very good fixation if you put the whole specimen in fixative and forget about it for a week or two. Some preliminary dissection is needed to aid fixation, and you're going to need some serious help with that. A valuable resource person on Histonet is John Kiernan, a pathologist turned research anatomist, and an expert on histologic technique. I hope he responds, otherwise try to find him. Bob Richmond Samurai Pathologist Maryville TN From michael.gudo at morphisto.de Sat Aug 22 03:36:45 2020 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Sat, 22 Aug 2020 10:36:45 +0200 Subject: [Histonet] human shoulder joint fixation In-Reply-To: References: Message-ID: <17F92B5F-D6DA-42B6-A83B-D51B65E80280@morphisto.de> Hello Merissa, You can use a diamond band saw, as they are produced by EXAKT. This special technique will cut soft tissue quite smooth without destroying the tissues. So you can cut slices with a few centimeter thickness which can be fixed in formaline after sectioning. What kind of embedding and sectioning / staining you are planing? You can embed the tissues some special resins which are suitable for larger specimens. If you need more information about the resin techniques and pathology saws, please contact me or Jerrod Roberts from EXAKT USA. Kind regards Michael > Am 21.08.2020 um 17:37 schrieb M.O. via Histonet : > > Happy Friday, Histonetters! > > I am doing some planning for a new project and wanted to get opinions on > fixation of large pieces of tissue. We will have human shoulders, where we > want to preserve the rotator cuff/joint. Cutting the tissue with a saw will > damage the soft tissue, so we were thinking that post-fixation would be > best for cutting slabs. > > Does anyone have experience with fixing such large pieces of tissue? We > typically use zinc buffered formalin for fixation. Would a vacuum work or a > vacuum sealer? > > Thank you for any input you might have!!!! > Sincerely, > Merissa > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************** MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From idimenstein at hotmail.com Sat Aug 22 13:07:43 2020 From: idimenstein at hotmail.com (Izak Dimenstein) Date: Sat, 22 Aug 2020 18:07:43 +0000 Subject: [Histonet] Histonet Digest, Vol 201, Issue 15 In-Reply-To: References: Message-ID: The rotator cuff/joint study is an interesting project. I wish I had an encounter with such a specimen before I?d written Grossing Bones: Principles, Techniques and Instruments book (Amazon.com). The problem is not in fixation, or even in a saw, but rather in getting the initial slab/s which preserve the intimate bone/soft tissue relationships. The key is in reliable immobilization before the cut. The technique is similar to a bone tumor with adjacent soft tissue. EXAKT is excellent, but I would start with a hand saw as more manageable. Then you would see how it goes (cost of the saw, space for it, etc.). For details idimenstein at hotmail.com. ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Saturday, August 22, 2020 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 201, Issue 15 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From carl.hobbs at kcl.ac.uk Sat Aug 22 14:28:43 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sat, 22 Aug 2020 19:28:43 +0000 Subject: [Histonet] human shoulder joint fixation Message-ID: Jeez....a human shoulder joint? Be grateful for background. Many years ago I had to fix/process to Polyester resin many monkey condylar joints ( jaw) As iterated here, I stripped all superfluous tissue off and processed the joints to resin. ( as I do now when doing Histology on ms knee joints as model for arthritis....but, using Pwax after decal) Monkey mandibles: fix in Formalin...on a rocker for 2 wks ( 20:1 Formalin:PBS) Changed twice I then took them specimens to a dedicated lab who sectioned this resin using a diamond circular saw. I was too young to follow the process further...also, in them days , Technicians did what they were told and were never appreciated. Maybe a different fix fluid will give a better morphology....JK will advise, surely! However, the project I was involved with won a prize...somewhere ( too young to be bothered) C Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From john.garratt at ciqc.ca Sat Aug 22 21:50:35 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Sun, 23 Aug 2020 02:50:35 +0000 Subject: [Histonet] Sausage controls In-Reply-To: <8be726b9bc4b4493a252c06588adf38b@seattlechildrens.org> References: <8be726b9bc4b4493a252c06588adf38b@seattlechildrens.org> Message-ID: A control block made of four tissues will cover the majority of IHC stains. Use appendix, tonsil, pancreas and liver in the block. It is important to select the tissues carefully to ensure they are fixed and processed appropriately. It is also important to test and document the tissues before adding them to the multi tissue block. If anybody needs more information on the testing and building protocol please contact me directly. John On Thu, Aug 20, 2020 at 11:12 AM, Sheeder, Christopher via Histonet wrote: > Here is the article... > Multitumor "Sausage" Blocks in Immunohistochemistry Simplified Method of Preparation, Practical Uses, and Roles in Quality Assurance > Rodney T. Miller, M.D., Carol L. Groothuis, MT(ASCP)SI > American Journal of Clinical Pathology, Volume 96, Issue 2, 1 August 1991, Pages 228-232, https://doi.org/10.1093/ajcp/96.2.228 > > Christopher Sheeder, HT(ASCP)CMQIHC > Histology Supervisor | Department of Laboratories > Seattle Children's Hospital > 4800 Sand Point Way NE > Seattle, WA 98105 > Office: 206-987-6259 > > christopher.sheeder at seattlechildrens.org > > Seattle Children's - Internal > > -----Original Message----- > From: Mac Donald, Jennifer > Sent: Wednesday, August 19, 2020 10:30 AM > To: Emily Horst > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Sausage controls > > The Journal of Histotechnology had an article regarding this years ago. Perhaps you can find it in the archives. Dr. Hector Battifora was the author. > Jennifer > > -----Original Message----- > From: Emily Horst via Histonet > Sent: Wednesday, August 19, 2020 10:11 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Sausage controls > > EXTERNAL SENDER- Exercise caution with requests, links, and attachments. > > Hello all, > > For those of you who use a sausage control for IHC/special stains, could you direct me as to process for starting that instead of using individual controls for everything? > > Thanks in advance! > > Emily > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIFAg&c=aBkXpkKi7gN5fe5MqrMaN-VmRugaRb1IDRfSv2xVRy0&r=c3bFZxewNEDI2wFyTyj_1jhuE1dV-f-i0gIzQyuiCw3aEzfs6mQm_0c0YpHyN0fx&m=fj4b9tOdVR0tBqtwawhTNGqy3ydx6AW8v-d9QPB0Af0&s=z_CRxXgU9gwTuxcX35Zf46MFZMgU7xAEwyXYef2jqGE&e= > > CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From edmartin26 at gmail.com Sun Aug 23 00:11:04 2020 From: edmartin26 at gmail.com (Eddie Martin) Date: Sun, 23 Aug 2020 01:11:04 -0400 Subject: [Histonet] Leica Bond, ASP6025 Processor parts In-Reply-To: <002a01d677e7$6f0fde70$4d2f9b50$@biopath.org> References: <002a01d677e7$6f0fde70$4d2f9b50$@biopath.org> Message-ID: <8CFA10E0-674A-487C-B86B-8DD6D1580B94@gmail.com> Hi Paula, Are you using the Leica Bond for Clinical diagnostic testing? If yes, use of any biomed company to PM or use non-Original equipment manufacturing (OEM) parts on the instrument would void the IVD status of your instrument and any IVD reagents you are automating on it. Sent from my iPhone >> On Aug 21, 2020, at 2:18 PM, Paula wrote: > ?Hi all, > > > > Does anyone know of a Biomed company that makes and/or sells parts for the > Bond III and the ASP6025 processor? > > > > It's amazing how much Leica wants for their parts. For example, the waste > bottle used to collect the buffer waste is $674 and the seal for the > retort's lid is $597. > > > > Hope someone can help..thanks in advance. > > > > Paula > > Bio-Path Medical Group > > Fountain Valley, CA From jkiernan at uwo.ca Sun Aug 23 11:51:45 2020 From: jkiernan at uwo.ca (John Kiernan) Date: Sun, 23 Aug 2020 16:51:45 +0000 Subject: [Histonet] human shoulder joint fixation In-Reply-To: References: , Message-ID: Thanks for the compliments, Bob, but I've no experience of trying to section anything as big and bony as a human shoulder joint. Car Hobbs and Izak Dimenstein probably will be able to give better advice to Merissa. Incidentally, I wasn't ever a pathologist. I moved from medicine to neuroanatomy (9-5 job!) at an early age. Some of my research could have been called experimental pathology. I also collaborated with some real neuropathologists, especially in the 1990s looking at the cerebral cortex and spinal cord in ALS and other motor neuron diseases. Cheers, John Kiernan. https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html = = = ________________________________ From: Bob Richmond via Histonet Sent: 21 August 2020 17:25 To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] human shoulder joint fixation > > Merissa (where?) asks; >>I am doing some planning for a new project and > wanted to get opinions on fixation of large pieces of tissue. We will have > human shoulders, where we want to preserve the rotator cuff/joint. Cutting > the tissue with a saw will damage the soft tissue, so we were thinking that > post-fixation would be best for cutting slabs. - Does anyone have > experience with fixing such large pieces of tissue? We typically use zinc > buffered formalin for fixation. Would a vacuum work or a vacuum sealer?<< > This is a non-trivial problem, and I hope you're working closely with the pathologist or other investigator who's going to be looking at the slides. Formalin (forget the zinc) penetrates tissue slowly enough that you're not going to get very good fixation if you put the whole specimen in fixative and forget about it for a week or two. Some preliminary dissection is needed to aid fixation, and you're going to need some serious help with that. A valuable resource person on Histonet is John Kiernan, a pathologist turned research anatomist, and an expert on histologic technique. I hope he responds, otherwise try to find him. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtighe at trudeauinstitute.org Mon Aug 24 12:13:54 2020 From: mtighe at trudeauinstitute.org (Mike Tighe) Date: Mon, 24 Aug 2020 17:13:54 +0000 Subject: [Histonet] McNeal's Trichrome recipe Message-ID: Hi everyone, I am trying to stain some tissues embedded in Osteo-bed. Does anyone have a recipe for McNeal's trichrome? Also I am having a tough time getting the sections to stay adhered to positive charged slides. Any recommendations? The sections are thin. Couple microns maybe. Thanks for any help! Mike From Nancy_Schmitt at pa-ucl.com Mon Aug 24 15:12:02 2020 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Mon, 24 Aug 2020 20:12:02 +0000 Subject: [Histonet] frozen section/histo duties Message-ID: Hello and Happy Monday- Would you please share what your processes are for: 1. HT's cutting frozen under direct supervision of pathologist - do you do this? * Now HT has changed and requires Associates degree - but previous would be grandfathered in - correct? 2. Non- certified staff working in histology with supervision - anything they DON"T do? CAP is our guideline and I am reading - want to make sure I am not overthinking this but not missing anything......... Thoughts appreciated, Nancy Schmitt HT, MLT(ASCP) Pathology Support Services Manager From kdean70 at hotmail.com Tue Aug 25 12:10:42 2020 From: kdean70 at hotmail.com (Ken M) Date: Tue, 25 Aug 2020 17:10:42 +0000 Subject: [Histonet] AFB blocks Message-ID: Does anyone know where I can obtain good Acid Fast Bacteria tissue blocks? Ken From Kelly.Pairan at nationwidechildrens.org Tue Aug 25 14:45:07 2020 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Tue, 25 Aug 2020 19:45:07 +0000 Subject: [Histonet] Dry Chemical Storage Message-ID: Good Afternoon, I am just wondering how other labs are storing their dry chemicals and powdered dyes. Are they in a special humidity controlled cabinet? Thanks, Kelly From Bonnie.Whitaker at osumc.edu Tue Aug 25 15:38:17 2020 From: Bonnie.Whitaker at osumc.edu (Whitaker, Bonnie) Date: Tue, 25 Aug 2020 20:38:17 +0000 Subject: [Histonet] slide printer Message-ID: Hi All, I am posting this for a friend. Any info would be appreciated. Any way we are looking at several slide printer options and wondered the following: 1) What are you using? 2) Did you first try any other vendors besides the one you are using now? 3) Do you like what you are using now? 4) Is it a one or two slide hopper - ie can you load and then select charged or non-charged slides? 5) Are you using vendor specific recommended slides or have you substituted for a cheaper or other option? 6) We are looking specifically at Leica, SlideMate and ESPO - do you have any experience of info from other users you know about these vendors? Any and all information you are able to share would be appreciated. Thanks, Bonnie Bonnie Whitaker AP Operations Director The Ohio State University Department of Pathology 614-293-8418 From Timothy.Morken at ucsf.edu Tue Aug 25 16:32:31 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 25 Aug 2020 21:32:31 +0000 Subject: [Histonet] slide printer In-Reply-To: References: Message-ID: When we implemented barcoding I was planning on using direct slide printing. It seemed the most efficient and safest way (ie, no mislabelling) to do the labeling. However, when we looked into getting the printers they were on the order of $9,000 per printer. And we wanted 20 printers for histology, cytology, grossing (frozens) and the kidney/muscle lab (kidney and muscle frozens) . That was a huge part of our equipment budget. In fact it was more than the computers, handheld scanners combined. So we started looking at slide label printers instead. They are far cheaper - on the order of $600 per unit. And we would need label printers for other areas as well - accessioning (six sites), grossing (three sites), consults slide/block labeling, cytology, EM, etc. We demoed both the direct slide printers and label printers. It was good to test them side by side because the pros and cons were very apparent. Besides the price of the direct slide printers we found they jammed often. They did not usually have an adequate output chute for the quantity slides we would print (often far more than the 5 or 10-slide capacity of the output chutes available. Also, we realized that since we use a few different slides types we would need to change slides while printing. That means segregating various types of labeled slides and somehow printing separately while needing to change out the slides each time. It was a logistical problem for the cutters. (this would be solved via centralized slide printing and routing different stain types to their correct slide type thru software. But we wanted to print at the microtome for best practice safe labeling of slides where they are cut). Then we found that if a the printed label on a slide was somehow damaged (smudged, scratched, etc) we would need to print a slide label to fix it. And how do you label a slide with a control tissue already mounted - it risks damaging the tissue section to run it thru a slide printer (and does the control have a label of some sort already?) Slide labels on the other hand were very flexible. We could print as many as needed right away (in literally a few seconds). Output quantity was not an issue. Any damaged could be reprinted right away. We could use any number of different types of slides to mount sections and apply the label we had. The cost of the labels would take 5 to 10 years to meet the cost of a direct slide printer (depending on how many labels any given printer produced per year, and by then you would probably move on to new printers anyway). We do use a direct slide printer for pre-mounted control slides. Then we put a printed label on the slide for staining with the specific stain necessary. The one issue I was concerned about was whether the techs would accept having to apply labels to every slide (which was previously done after staining by one person - with many mistakes!). But during testing and implementation we had every tech try out both systems and they did not mind putting on labels. They thought it was far better than the hand-writing they had been doing. When we went live no one ever complained about putting labels on slides. We use the same labels for slides, requisitions and containers so no extra cost for different kinds of labels and printers. 1) What are you using? Cognitive label printers from General Data and StainerShield labels (survive every known histology chemical we have tried, including antigen retrieval). 2) Did you first try any other vendors besides the one you are using now? General Data (printed labels), ThermoFisher SlideMate, ShurMark (etcher - very slow printing) (we tried other printed label makers as well but GD was the best by far). I will note that when using printed labels you must use a matched system - the labels and printers from each vendor are designed to work together for the ideal results. Mixing and matching printers and labels is very difficult. Since the vendor has already done that, use their system (take it from someone who tried to do that!). 3) Do you like what you are using now? Very much. It works perfectly. 4) Is it a one or two slide hopper - ie can you load and then select charged or non-charged slides? No need with printed labels. 5) Are you using vendor specific recommended slides or have you substituted for a cheaper or other option? We use various slides. Labels can go on any slide. However, with label printing you must used the matched system a vendor offers - label and printer are designed to work together for best results. 6) We are looking specifically at Leica, SlideMate and ESPO - do you have any experience of info from other users you know about these vendors? General Data. Absolutely excellent. I suggest making a table with each printer or labeler in the columns and then a list on the left of the key features you want, or they have. Then compare each one. Prioritize your most desired features (including price!). Have the key people score each one. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Whitaker, Bonnie via Histonet Sent: Tuesday, August 25, 2020 1:38 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] slide printer Hi All, I am posting this for a friend. Any info would be appreciated. Any way we are looking at several slide printer options and wondered the following: 1) What are you using? 2) Did you first try any other vendors besides the one you are using now? 3) Do you like what you are using now? 4) Is it a one or two slide hopper - ie can you load and then select charged or non-charged slides? 5) Are you using vendor specific recommended slides or have you substituted for a cheaper or other option? 6) We are looking specifically at Leica, SlideMate and ESPO - do you have any experience of info from other users you know about these vendors? Any and all information you are able to share would be appreciated. Thanks, Bonnie Bonnie Whitaker AP Operations Director The Ohio State University Department of Pathology 614-293-8418 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=18g-wVk5i-6n_slwSEACqnjBrPxPAtiene9uRo6tlvQ&s=i_n4itaiddJ5GNyevVc-M6XUUvNI9Ij34j3A0rYrjpA&e= From Andrew.Dilts at coxhealth.com Wed Aug 26 07:20:29 2020 From: Andrew.Dilts at coxhealth.com (Dilts,Andrew) Date: Wed, 26 Aug 2020 12:20:29 +0000 Subject: [Histonet] frozen section/histo duties In-Reply-To: References: Message-ID: It's a little tricky and usually brings in CLIA's definition of high complexity testing personnel and then the nuanced definition of testing. As strange as it seems, most of what we do in histology is not actually testing until a pathologist reads it out and reviews it. The way I read it, the only things in pathology that are actually high complexity testing that aren't performed exclusively by pathologists are grossing (ANP.11610) and QCing special stain/ISH/IHC slides (ANP.21395). This can put some labs in a position where, even though a grandfathered histotech may be very capable at QCing stains and fully certified, he or she may not be able to do the QC for regulatory purposes. Furthermore, the requirements to sit for the exam could be met without being CLIA high complexity testing compliant, so we are still pumping out new histotechs in this loophole. I doubt it was intended that way, but there it is. Other high complexity tasks include report review (ANP.29590), digital image analysis (ANP.23038), and circulating tumor cell analysis (ANP.29630) but those aren't an issue for our lab because we either don't do them or because our pathologists obviously do report review. More directly to your point, I don't see anything in the requirements about histotechs doing or not doing frozens. I also don't see anything about requiring histotechs to be certified at all for any reason other than your laboratory's preference. In fact, you can have non-certified staff that have the high complexity testing education who are able to perform routine histology tasks that certified (particularly grandfathered) histotechs aren't allowed to do. Hopefully someone with more knowledge will chime in because I'm sure I have a lot to learn about the ins and outs of this. Andrew Dilts HTL(ASCP) Histo Supervisor, Laboratory Services Phone: (417) 269-5021 Andrew.Dilts at coxhealth.com www.coxhealth.com -----Original Message----- From: Nancy Schmitt Sent: Monday, August 24, 2020 3:12 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] frozen section/histo duties Hello and Happy Monday- Would you please share what your processes are for: 1. HT's cutting frozen under direct supervision of pathologist - do you do this? * Now HT has changed and requires Associates degree - but previous would be grandfathered in - correct? 2. Non- certified staff working in histology with supervision - anything they DON"T do? CAP is our guideline and I am reading - want to make sure I am not overthinking this but not missing anything......... Thoughts appreciated, Nancy Schmitt HT, MLT(ASCP) Pathology Support Services Manager CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intendedrecipient(s) and may contain confidential and privileged information protected by law. ?Any unauthorized review, use, disclosure or distribution is prohibited.?If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Timothy.Morken at ucsf.edu Wed Aug 26 10:24:52 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 26 Aug 2020 15:24:52 +0000 Subject: [Histonet] frozen section/histo duties In-Reply-To: References: Message-ID: " The way I read it, the only things in pathology that are actually high complexity testing that aren't performed exclusively by pathologists are .... and QCing special stain/ISH/IHC slides (ANP.21395). " CLIA regulations specifically state that quality control is not a test so it is not covered by the complexity rules. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Dilts,Andrew via Histonet Sent: Wednesday, August 26, 2020 5:20 AM To: 'Nancy Schmitt' ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] frozen section/histo duties It's a little tricky and usually brings in CLIA's definition of high complexity testing personnel and then the nuanced definition of testing. As strange as it seems, most of what we do in histology is not actually testing until a pathologist reads it out and reviews it. The way I read it, the only things in pathology that are actually high complexity testing that aren't performed exclusively by pathologists are grossing (ANP.11610) and QCing special stain/ISH/IHC slides (ANP.21395). This can put some labs in a position where, even though a grandfathered histotech may be very capable at QCing stains and fully certified, he or she may not be able to do the QC for regulatory purposes. Furthermore, the requirements to sit for the exam could be met without being CLIA high complexity testing compliant, so we are still pumping out new histotechs in this loophole. I doubt it was intended that way, but there it is. Other high complexity tasks include report review (ANP.29590), digital image analysis (ANP.23038), and circulating tumor cell analysis (ANP.29630) but those aren't an issue for our lab because we either don't do them or because our pathologists obviously do report review. More directly to your point, I don't see anything in the requirements about histotechs doing or not doing frozens. I also don't see anything about requiring histotechs to be certified at all for any reason other than your laboratory's preference. In fact, you can have non-certified staff that have the high complexity testing education who are able to perform routine histology tasks that certified (particularly grandfathered) histotechs aren't allowed to do. Hopefully someone with more knowledge will chime in because I'm sure I have a lot to learn about the ins and outs of this. Andrew Dilts HTL(ASCP) Histo Supervisor, Laboratory Services Phone: (417) 269-5021 Andrew.Dilts at coxhealth.com https://urldefense.proofpoint.com/v2/url?u=http-3A__www.coxhealth.com&d=DwIGaQ&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=LXTqkewz9k7aOX8GHkE9kjIWImSbkPcE-HZ9PGP6VX8&s=CwylMDTvTcTJPlPEssSfefVCw86sgGdHl08WdVY4DnI&e= -----Original Message----- From: Nancy Schmitt Sent: Monday, August 24, 2020 3:12 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] frozen section/histo duties Hello and Happy Monday- Would you please share what your processes are for: 1. HT's cutting frozen under direct supervision of pathologist - do you do this? * Now HT has changed and requires Associates degree - but previous would be grandfathered in - correct? 2. Non- certified staff working in histology with supervision - anything they DON"T do? CAP is our guideline and I am reading - want to make sure I am not overthinking this but not missing anything......... Thoughts appreciated, Nancy Schmitt HT, MLT(ASCP) Pathology Support Services Manager CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intendedrecipient(s) and may contain confidential and privileged information protected by law. ?Any unauthorized review, use, disclosure or distribution is prohibited.?If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=LXTqkewz9k7aOX8GHkE9kjIWImSbkPcE-HZ9PGP6VX8&s=anC721-uLKpcQJ2VCdaqTq4NVWDW24HrvSTsYvDn_aE&e= From Timothy.Morken at ucsf.edu Wed Aug 26 11:29:45 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 26 Aug 2020 16:29:45 +0000 Subject: [Histonet] frozen section/histo duties In-Reply-To: References: Message-ID: Nancy, we have HT (Histotechs) and HLT (Hospital lab techs - lab assistants) who work in grossing and do the frozen sections with residents and pathologists. There is no requirement that a person cutting sections has to be certified in anything. Our HLT's generally do all the accessioning but do frozens when it gets busy. However, they do not select the piece to freeze - that is done by a PA or resident. They just cut the sections and stain them. We have a similar situation in histology in that we will hire well qualified people (ie, degrees in biology, or other science) with no experience as HLT's and then train them into histotechs eventually. But in the meantime they can be doing many histotech duties like embedding, sectioning, H&E staining, helping with IHC stainers, etc. Many of these HLT's have become certified techs in our lab over the years. We have 15 histotechs and all are certified. Our 4 HLT's are not. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Nancy Schmitt via Histonet Sent: Monday, August 24, 2020 1:12 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] frozen section/histo duties Hello and Happy Monday- Would you please share what your processes are for: 1. HT's cutting frozen under direct supervision of pathologist - do you do this? * Now HT has changed and requires Associates degree - but previous would be grandfathered in - correct? 2. Non- certified staff working in histology with supervision - anything they DON"T do? CAP is our guideline and I am reading - want to make sure I am not overthinking this but not missing anything......... Thoughts appreciated, Nancy Schmitt HT, MLT(ASCP) Pathology Support Services Manager _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=ZFDDncvaq-1TYxY5Fb-qHd8CTU146jN-KxsQd7hTC24&s=v9AjGrHjBheNgRP_nQpZi5Aioo1vaTo9KNC-reYP71g&e= From Karen.Heckford at DignityHealth.org Thu Aug 27 06:28:37 2020 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Thu, 27 Aug 2020 11:28:37 +0000 Subject: [Histonet] Hiring per diem in SF Message-ID: Good Morning, I have an opening for a per diem position. Right now it is for at least 8 hours per week but can develop into more hours. Apply Dignity Health St. Mary's Medical Center St. Mary's Medical Center San Francisco. If you have any questions email me. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. From amyleehisto779 at gmail.com Thu Aug 27 12:21:59 2020 From: amyleehisto779 at gmail.com (Amy Lee) Date: Thu, 27 Aug 2020 10:21:59 -0700 Subject: [Histonet] NIS Elements Message-ID: Hello, We used to use Image-Pro Premier to capture and analyze images. Does anyone use NIS Elements with Nikon to do image capture and analyze? How do you feel about it? If you used both of them, could you let me know what you think? We need to make a decision for a new purchase. Thank you, Amy From jwhite at esu.edu Fri Aug 28 12:43:27 2020 From: jwhite at esu.edu (Jennifer White) Date: Fri, 28 Aug 2020 17:43:27 +0000 Subject: [Histonet] Histonet Digest - Nikon NIS Elements Message-ID: <2F49FD17-BACD-473E-8EFD-42197635B78A@esu.edu> Amy, I have been using Nikon's NIS Elements D imaging software for several years along with a Nikon DS-Fi1 digital camera to capture images of various histological slides, as well as small whole-mount specimens on a dissecting scope. I have been happy with its ease of use and the images that it captures. I have found it to be fairly user-friendly and easy to manipulate, at least for my purposes. You do have to calibrate it with your particular scope to take measurements, and I required some tech support to do so. There is a nice set of annotation and measurement tools including length, area, radius, counts, and angles. I have experienced minor trouble exporting data into Excel, but with some fussing I have been able to successfully create data files from measurements. I have used images and measurements in both teaching and research, and would recommend the software for basic image capturing and analysis. Jennifer White East Stroudsburg University ?On 8/28/20, 1:00 PM, "histonet-request at lists.utsouthwestern.edu" wrote: Today's Topics: 1. NIS Elements (Amy Lee) ---------------------------------------------------------------------- Message: 1 Date: Thu, 27 Aug 2020 10:21:59 -0700 From: Amy Lee To: histonet at lists.utsouthwestern.edu Subject: [Histonet] NIS Elements Message-ID: Content-Type: text/plain; charset="UTF-8" Hello, We used to use Image-Pro Premier to capture and analyze images. Does anyone use NIS Elements with Nikon to do image capture and analyze? How do you feel about it? If you used both of them, could you let me know what you think? We need to make a decision for a new purchase. Thank you, Amy ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 201, Issue 20 ***************************************** From susanhay59 at gmail.com Sat Aug 29 06:21:46 2020 From: susanhay59 at gmail.com (S hay) Date: Sat, 29 Aug 2020 06:21:46 -0500 Subject: [Histonet] Cancel Message-ID: Please remove me from your email list and confirm when task completed. Ty so much. Susan Hay From Kelly.Pairan at nationwidechildrens.org Mon Aug 31 14:30:08 2020 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Mon, 31 Aug 2020 19:30:08 +0000 Subject: [Histonet] Rhabdomyosarcoma Control Tissue Message-ID: <1c5d044459474110b8ad4212af128faf@l1perdwmbx01.childrensroot.net> Good Afternoon, We are currently looking for some rhabdomyosarcoma control tissue. All of our cases that have a significant amount of tissue have post treatment necrosis. We would be happy to share anything we have that you might need. Thanks for your help, Kelly