From Melissa.Kuhnla at chsli.org Wed Apr 1 12:05:59 2020 From: Melissa.Kuhnla at chsli.org (Kuhnla, Melissa) Date: Wed, 1 Apr 2020 17:05:59 +0000 Subject: [Histonet] Grossing SOP Message-ID: <3b42e1d45c12442280649c3e93155f75@chsli.org> Good Afternoon, Hope this finds everyone healthy and well. I am helping my laboratory with a project centered around an SOP for grossing specimens (for both PAs and techs). We are in New York state. I am pushing this working group to write an SOP in general terms and to include any compliance items... leave the 'how to' grossing instructions for every specimen type to an appendix or linked document to not bog the SOP down with too much detail. I have looked through Joint Commission and NYSDOH and find very little compliance items related to this 'grossing' function. How do ya'll handle this in your manuals? Thank you kindly and stay well. Melissa Kuhnla Lead Medical Technologist for IHC and FISH testing Regional Laboratory Services Good Samaritan Hospital 631-609-2551 From aperl at caremount.com Wed Apr 1 13:17:17 2020 From: aperl at caremount.com (Perl, Alison) Date: Wed, 1 Apr 2020 18:17:17 +0000 Subject: [Histonet] Grossing SOP In-Reply-To: <3b42e1d45c12442280649c3e93155f75@chsli.org> References: <3b42e1d45c12442280649c3e93155f75@chsli.org> Message-ID: <8d1db4f0e46243aa9b0e163a4edb419b@MK-EXMB02.mkmg.com> Hi Melissa I'm also in NY, and do something similar - we have an SOP with basic workflow and guidelines, then have attachments with specific protocols and instructions Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at caremount.com -----Original Message----- From: Kuhnla, Melissa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 1, 2020 1:06 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] Grossing SOP Good Afternoon, Hope this finds everyone healthy and well. I am helping my laboratory with a project centered around an SOP for grossing specimens (for both PAs and techs). We are in New York state. I am pushing this working group to write an SOP in general terms and to include any compliance items... leave the 'how to' grossing instructions for every specimen type to an appendix or linked document to not bog the SOP down with too much detail. I have looked through Joint Commission and NYSDOH and find very little compliance items related to this 'grossing' function. How do ya'll handle this in your manuals? Thank you kindly and stay well. Melissa Kuhnla Lead Medical Technologist for IHC and FISH testing Regional Laboratory Services Good Samaritan Hospital 631-609-2551 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GauchV at amc.edu Fri Apr 3 11:07:28 2020 From: GauchV at amc.edu (Gauch, Vicki) Date: Fri, 3 Apr 2020 16:07:28 +0000 Subject: [Histonet] Metal Testing Message-ID: Hi everyone, Does anyone know of a laboratory that can identify the type of metal in a foreign body ? I need a lab that is licensed to do testing in NY state. I have already tried LabCorp, MAYO and NMS. Any help would be greatly appreciated. Thank you, Vicki Gauch AMCH Pathology Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From paula at excaliburpathology.com Fri Apr 3 11:30:07 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Fri, 3 Apr 2020 16:30:07 +0000 (UTC) Subject: [Histonet] Metal Testing In-Reply-To: References: Message-ID: <948693009.113452.1585931407349@mail.yahoo.com> A lab with X-Ray diffraction on an SEM could help. Try the university electron microscopy labs. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Friday, April 3, 2020, 11:14:30 AM CDT, Gauch, Vicki via Histonet wrote: Hi everyone, Does anyone know of a laboratory that can identify the type of metal in a foreign body ? I need a lab that is licensed to do testing in NY state.? I have already tried LabCorp, MAYO and NMS.? Any help would be greatly appreciated. Thank you, Vicki Gauch AMCH Pathology Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtoole at dcol.net Fri Apr 3 12:13:27 2020 From: mtoole at dcol.net (Mike Toole) Date: Fri, 3 Apr 2020 12:13:27 -0500 Subject: [Histonet] Metal Testing Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB35E1BCBE4EC@mail> Does the lab have to be licensed (to perform testing on human specimens such as blood, body fluids, tissues and cells) in order to perform qualitative analytical testing on a metal object that is foreign to the human body? There are many labs that can do qualitative analysis of metals. An Atomic Absorption Spectrophotometer is a good instrument for that purpose. In a quick Google search I found Avomeen Analytical Services 833-507-6834. FDA-Registered and DEA-Licensed (schedules 1-5), GLP/GMP-compliant, and ISO 17025 accredited. Mike -----Original Message----- From: Paula Keene Pierce via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, April 03, 2020 11:30 AM To: histonet at lists.utsouthwestern.edu; Gauch, Vicki Subject: [EXTERNAL] Re: [Histonet] Metal Testing A lab with X-Ray diffraction on an SEM could help. Try the university electron microscopy labs. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Friday, April 3, 2020, 11:14:30 AM CDT, Gauch, Vicki via Histonet wrote: Hi everyone, Does anyone know of a laboratory that can identify the type of metal in a foreign body ? I need a lab that is licensed to do testing in NY state.? I have already tried LabCorp, MAYO and NMS.? Any help would be greatly appreciated. Thank you, Vicki Gauch AMCH Pathology Albany, NY ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague at pathologyarts.com Sat Apr 4 20:06:46 2020 From: c.tague at pathologyarts.com (Curt Tague) Date: Sun, 5 Apr 2020 01:06:46 +0000 Subject: [Histonet] too much formalin time Message-ID: Quick drive by question, We have some tonsil that has been in formalin for about 2 months, to be disposed of, is that not recommended for use as control tissue now, too much formalin exposure? Thanks for your input again, Curt From carl.hobbs at kcl.ac.uk Sun Apr 5 13:39:25 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sun, 5 Apr 2020 18:39:25 +0000 Subject: [Histonet] too much formalin time (Curt Tague) Message-ID: Hi Curt I trust you and yours are well... Imho.....it will still be an excellent control, provided that you are processing it to Paraffin wax and use HIER ( antigen retrieval) before IHC/IF. Sure, as long as it has been optimally fixed ( immersion fixation with no agitation -BAD- leads to zonal variations of positivity) I have posted images on Histonet site of human tonsil that has remained in fix for a similar time before processing. Sure, I cannot guarantee all proteins will be retrievable but, in my XP, good fixation is the key....not the length of fixation. Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From ranna0726 at gmail.com Mon Apr 6 18:36:45 2020 From: ranna0726 at gmail.com (Ranna Mehta) Date: Mon, 6 Apr 2020 16:36:45 -0700 Subject: [Histonet] question about Digital Pathology Certificate Message-ID: Hi All, I need inputs from my histo colleagues about Digital Pathology certification from NSH. If anyone has done this program, how is it helping in their career? what are pros and cons? Do they include MatLab and R programming in syllabus? I know it is very resourceful in research lab but what about Clinical labs? Right now i am working on multiplex staining using Vectra inform and Halo. I am self learner; I don't have any help except company's technical support. I am wondering- attending this program will help me understanding algorithm and analysis? Thanks in advanced. Stay safe. sorry for lots of questions. Regards Ranna Mehta Associate Scientist From jkiernan at uwo.ca Mon Apr 6 19:13:18 2020 From: jkiernan at uwo.ca (jkiernan at uwo.ca) Date: Tue, 7 Apr 2020 00:13:18 +0000 Subject: [Histonet] On-line references In-Reply-To: References: , Message-ID: Just for the record, there's a 5th edition (2015), ISBN 9781907904325. (The 4th edition was in 2008.) John Kiernan. = = = ________________________________ From: jon.st.onge at agilent.com Sent: 06 April 2020 17:40 To: John Kiernan ; Tom Wells Subject: RE: On-line references There is also a wonderful book called: Histological and Histochemical Methods; Theory and Practice, 4th Edition, by one J.A. Kiernan that I would highly recommend (and it's at a very good price). I'd be happy to give you more information if needed. Happy Monday, Jon -----Original Message----- From: John Kiernan via Histonet Sent: Wednesday, March 25, 2020 11:12 PM To: histonet at lists.utsouthwestern.edu; Tom Wells Subject: Re: [Histonet] On-line references Hello, Tom. Some old classics are there for free, most notably JR Baker's "Principles of Biological Microtechnique" (1958), but almost anything more recent has to be bought. There are plenty of cheap older editions of histotechnology books on sites like AbeBooks. Check it out for the last edition of Pearse's Histochemistry! I was amazed. Even the latest editions of books in our field cost only about $100 from the publisher and most are good for several years. Compare this with the price of a few drops of an antibody or (more realistically) a staining machine in which you must only use the liquids sold by its vendor. John Kiernan = = = ________________________________ From: Tom Wells via Histonet > Sent: 25 March 2020 14:34 To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] On-line references Given that our Institute's library is closed due to the pandemic, is anyone aware of on-line versions of Histotechnology/ Histochemistry textbooks? Thanks. Tom Tom Wells BSc, MEd, MLT, ART Faculty Medical Laboratory Science School of Health Sciences SW03-3088 (604) 412-7594 BCIT _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Mon Apr 6 19:46:14 2020 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue, 7 Apr 2020 00:46:14 +0000 Subject: [Histonet] On-line references In-Reply-To: References: , Message-ID: <77f187aa7d2740afae00856652101b96@SVDCMBX-MEX024.nswhealth.net> And each edition gets better (and better). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: jkiernan--- via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, 7 April 2020 10:13 AM To: jon.st.onge at agilent.com; Tom Wells Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] On-line references Just for the record, there's a 5th edition (2015), ISBN 9781907904325. (The 4th edition was in 2008.) John Kiernan. = = = ________________________________ From: jon.st.onge at agilent.com Sent: 06 April 2020 17:40 To: John Kiernan ; Tom Wells Subject: RE: On-line references There is also a wonderful book called: Histological and Histochemical Methods; Theory and Practice, 4th Edition, by one J.A. Kiernan that I would highly recommend (and it's at a very good price). I'd be happy to give you more information if needed. Happy Monday, Jon -----Original Message----- From: John Kiernan via Histonet Sent: Wednesday, March 25, 2020 11:12 PM To: histonet at lists.utsouthwestern.edu; Tom Wells Subject: Re: [Histonet] On-line references Hello, Tom. Some old classics are there for free, most notably JR Baker's "Principles of Biological Microtechnique" (1958), but almost anything more recent has to be bought. There are plenty of cheap older editions of histotechnology books on sites like AbeBooks. Check it out for the last edition of Pearse's Histochemistry! I was amazed. Even the latest editions of books in our field cost only about $100 from the publisher and most are good for several years. Compare this with the price of a few drops of an antibody or (more realistically) a staining machine in which you must only use the liquids sold by its vendor. John Kiernan = = = ________________________________ From: Tom Wells via Histonet > Sent: 25 March 2020 14:34 To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] On-line references Given that our Institute's library is closed due to the pandemic, is anyone aware of on-line versions of Histotechnology/ Histochemistry textbooks? Thanks. Tom Tom Wells BSc, MEd, MLT, ART Faculty Medical Laboratory Science School of Health Sciences SW03-3088 (604) 412-7594 BCIT _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From bakevictoria at gmail.com Wed Apr 8 06:39:14 2020 From: bakevictoria at gmail.com (Victoria Baker) Date: Wed, 8 Apr 2020 07:39:14 -0400 Subject: [Histonet] question about Digital Pathology Certificate In-Reply-To: References: Message-ID: Ranna, I took the course and it was well worth the cost for me. As to how it is assisting my career right now it isn't as my facility stopped using imaging about a year and a half ago. With the pandemic they may be rethinking this, but it won't be immediate. This type of skill will be more important, I believe, with the advancement of telemedicine and what we should be learning from the COVID-19 shut down or limited resources of certain services. Plus with so many facilities merging to survive in the current environment this is going to be necessary. What I would like to see is more follow up courses and more attention drawn to this program. Vikki On Mon, Apr 6, 2020, 7:48 PM Ranna Mehta via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi All, > I need inputs from my histo colleagues about Digital Pathology > certification from NSH. If anyone has done this program, how is it helping > in their career? > what are pros and cons? Do they include MatLab and R programming in > syllabus? I know it is very resourceful in research lab but what about > Clinical labs? > Right now i am working on multiplex staining using Vectra inform and Halo. > I am self learner; I don't have any help except company's technical > support. I am wondering- attending this program will help me understanding > algorithm and analysis? Thanks in advanced. Stay safe. > sorry for lots of questions. > Regards > Ranna Mehta > Associate Scientist > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ranna0726 at gmail.com Wed Apr 8 09:05:53 2020 From: ranna0726 at gmail.com (Ranna) Date: Wed, 8 Apr 2020 07:05:53 -0700 Subject: [Histonet] question about Digital Pathology Certificate In-Reply-To: References: Message-ID: Thank you Victoria Regards Ranna Sent from my iPhone > On Apr 8, 2020, at 4:39 AM, Victoria Baker wrote: > > ? > Ranna, > > I took the course and it was well worth the cost for me. As to how it is assisting my career right now it isn't as my facility stopped using imaging about a year and a half ago. With the pandemic they may be rethinking this, but it won't be immediate. > This type of skill will be more important, I believe, with the advancement of telemedicine and what we should be learning from the COVID-19 shut down or limited resources of certain services. Plus with so many facilities merging to survive in the current environment this is going to be necessary. > > What I would like to see is more follow up courses and more attention drawn to this program. > > Vikki > > > >> On Mon, Apr 6, 2020, 7:48 PM Ranna Mehta via Histonet wrote: >> Hi All, >> I need inputs from my histo colleagues about Digital Pathology >> certification from NSH. If anyone has done this program, how is it helping >> in their career? >> what are pros and cons? Do they include MatLab and R programming in >> syllabus? I know it is very resourceful in research lab but what about >> Clinical labs? >> Right now i am working on multiplex staining using Vectra inform and Halo. >> I am self learner; I don't have any help except company's technical >> support. I am wondering- attending this program will help me understanding >> algorithm and analysis? Thanks in advanced. Stay safe. >> sorry for lots of questions. >> Regards >> Ranna Mehta >> Associate Scientist >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdean70 at hotmail.com Wed Apr 8 14:50:09 2020 From: kdean70 at hotmail.com (Ken M) Date: Wed, 8 Apr 2020 19:50:09 +0000 Subject: [Histonet] Question concerning H-Pylori Staining Message-ID: Hi everyone! We have a question about staining for H-Pylori Using Quick Stain (Periodic acid 1%, Alician Yellow, Sodium Metabisulfate,Toluidine Blue stock, Sodium Hydroxide) we notice what clearly looks like the H-Pylori purple stained clusters, but after dehydration in 100% alcohol the purple clusters seem to disappear. Should we just dehydrate using a slide oven instead of the alcohol? For how long at what temp? Could the alcohol be affecting the purple color making it too light to see? Michelle From relia1 at earthlink.net Thu Apr 9 10:15:47 2020 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 9 Apr 2020 11:15:47 -0400 Subject: [Histonet] Is there anything I can do for you? Message-ID: <008901d60e81$bf898880$3e9c9980$@earthlink.net> Hi Histonetters, I hope you and your loved ones are staying safe and healthy, and you can take time to enjoy Easter, Passover and this season with your family. It seems that things are changing on an almost daily basis. I have been monitoring social media - personal connections and histology related groups on Facebook and LinkedIN and have been staying in touch with many of my active candidates and customers. I have gotten a lot of questions about what is going on with the ?career? aspect of histology at this time. Here is a summary of what I have observed and experienced so far: Histotechs supporting ?non-essential surgeries? i.e. Derm and GI private practices: ? Some are experiencing cuts in hours and/or are using the time to catch up on backlogs and lab maintenance. ? Many are being furloughed with the expectation of being called back to work once ?the all clear is given?, ? Start dates for new employees are being postponed until further notice. Traveling Histotechs - Histotechs that are working as contractors nationwide: ? Most of the travel techs I have heard from have been grounded meaning sent home until further notice. ? They seem confident that they will be returning to their assignments as they are looking for prn positions for now. Hospital based histotechs: ? Some are being furloughed ? Some are having their hours reduced ? Some are job sharing meaning for example if they have a regular staff of 4 techs they take turns 2 at a time working for a week while the other 2 stay home ? Some are being used in other areas for example if they have experience in phlebotomy they are moving to that department. I can?t really speak to research, academic or pharmaceutical areas as I haven?t heard much from them. (Hopefully that?s because they are busy working on the COVID-19 response.) What I glean from this and bear in mind this only an opinion on my part based on what I have heard anecdotally. It seems that the best case scenario at this point is there was a shortage of histotechs before the pandemic and there will be just as great a need after the pandemic in other words we are just pressing pause. Just Remember Things Will Get Better. This Will End. & We Will Return To Our Normal Lives. In the meantime Is there anything I can do for you? Were you actively looking for a new position before this crisis? Are you someone who is unemployed as opposed to furloughed? Are you feeling lonely or isolated and just need to hear a friendly voice? Call Me, Text Me, Email Me, Or Contact Me through Social Media. We are all in this together Looking forward to the light at the end of the tunnel. Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! From criley at dpspa.com Thu Apr 9 11:47:44 2020 From: criley at dpspa.com (Charles Riley) Date: Thu, 9 Apr 2020 12:47:44 -0400 Subject: [Histonet] Competency questionnaires Message-ID: Does anyone use a questionnaire for testing a techs competency either by itself or on top of practical application of the skills needed? If so would you mind sharing with me your questions (I am looking for grossing, microtomy and troubleshooting, embedding, processing, and immunohistochemistry/special/H&E staining and troubleshooting. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From rsrichmond at gmail.com Thu Apr 9 13:05:08 2020 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 9 Apr 2020 14:05:08 -0400 Subject: [Histonet] Question concerning H. pylori staining In-Reply-To: References: Message-ID: Michelle (where) asksi: >>We have a question about staining for H-Pylori Using Quick Stain (Periodic acid 1%, Alician Yellow, Sodium Metabisulfate, Toluidine Blue stock, Sodium Hydroxide) we notice what clearly looks like the H-Pylori purple stained clusters, but after dehydration in 100% alcohol the purple clusters seem to disappear. Should we just dehydrate using a slide oven instead of the alcohol? For how long at what temp? Could the alcohol be affecting the purple color making it too light to see?<< You identify Helicobacter pylori by its morphology - curved, angled, or "gull wing" bacilli. Is that what you're seeing? If you do this stain, you should know how it's interpreted. The Alcian yellow (correct spelling) method is needlessly complex. What does the periodic acid do? - A solution of toluidine blue (Diff-Quik 2 or a generic equivalent) suffices - don't use Diff-Quik 1 with it - dehydrate through alcohols rapidly. Bob Richmond Samurai Pathologist Maryville TN From gu.lang at gmx.at Sun Apr 12 03:38:29 2020 From: gu.lang at gmx.at (Gudrun Lang) Date: Sun, 12 Apr 2020 10:38:29 +0200 Subject: [Histonet] Question concerning H. pylori staining In-Reply-To: References: Message-ID: <000901d610a5$bec8d790$3c5a86b0$@gmx.at> Hi! I found this instruction for a Hp- stain, that sounds similiar to yours. They want the slides to be airdried after water-rinsing and before xylen. But the result should be blue bacteria, not purple. I would try to let the slides air-dry for about half a minute, then rinse very-very short in absolute ethanol (one dip). The colour should turn blue and some dye will be extracted to give a clearer result. Then fast into xylen, dip a few times, then coverslip. http://www.helicostat.com/helicostat_instructions.html I assume, that the periodic acid should render some mucins stainable with Alcian yellow, to give a more contrasted result. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Donnerstag, 9. April 2020 20:05 An: Histonet at lists.utsouthwestern.edu Betreff: Re: [Histonet] Question concerning H. pylori staining Michelle (where) asksi: >>We have a question about staining for H-Pylori Using Quick Stain (Periodic acid 1%, Alician Yellow, Sodium Metabisulfate, Toluidine Blue stock, Sodium Hydroxide) we notice what clearly looks like the H-Pylori purple stained clusters, but after dehydration in 100% alcohol the purple clusters seem to disappear. Should we just dehydrate using a slide oven instead of the alcohol? For how long at what temp? Could the alcohol be affecting the purple color making it too light to see?<< You identify Helicobacter pylori by its morphology - curved, angled, or "gull wing" bacilli. Is that what you're seeing? If you do this stain, you should know how it's interpreted. The Alcian yellow (correct spelling) method is needlessly complex. What does the periodic acid do? - A solution of toluidine blue (Diff-Quik 2 or a generic equivalent) suffices - don't use Diff-Quik 1 with it - dehydrate through alcohols rapidly. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From le_tok at mail.ru Sun Apr 12 09:45:55 2020 From: le_tok at mail.ru (=?UTF-8?B?0JXQu9C10L3QsCDQmNCy0LvQtdCy0LA=?=) Date: Sun, 12 Apr 2020 17:45:55 +0300 Subject: [Histonet] (no subject) Message-ID: <1586702755.895107852@f170.i.mail.ru> I want to unsubscribe? ? ? ? ?????????, ?????? ?????. From jkiernan at uwo.ca Mon Apr 13 01:34:29 2020 From: jkiernan at uwo.ca (jkiernan at uwo.ca) Date: Mon, 13 Apr 2020 06:34:29 +0000 Subject: [Histonet] Question concerning H. pylori staining In-Reply-To: <000901d610a5$bec8d790$3c5a86b0$@gmx.at> References: , <000901d610a5$bec8d790$3c5a86b0$@gmx.at> Message-ID: In the method of Leung et al. (1996 J. Histotechnol. 19:131), the periodic acid oxidizes the neutral gastric mucus to generate aldehyde groups, as in the first part of the PAS method. Treatment with an acidified bisulphite solution then converts the aldehyde groups to aldehyde bifulphite groups, which are strong anions. Alcian yellow (a cationic azo dye) binds strongly to these anionic sites (but not to nucleic acids) and may then change into a permanently insoluble pigment. (The chemistry is similar to that of staining with alcian blue 8G; this isn't the place to discuss all that!) The next step (mildly alkaline aqueous toluidine blue) imparts blue to nearly everything not already coloured yellow; it cannot displace alcian yellow from the already stained neutral gastric mucus, making for strong contrast. The bacteria stain blue by virtue of their anionic components (nucleic acids and materials in their cell walls) so do nuclei of cells. Toluidine blue, like most dyes with small molecules, stains quickly and is also easy to remove. Alcohol-water mixtures remove such dyes faster than either water or 100% alcohol. The original Leung method recommended removing most of the water by careful blotting, and then taking the slide(s) directly to the first of 3 changes of 100% alcohol. Real alcian yellow (CI 12840, Ingrain yellow 1) has not been manufactured for many years. Other methods for showing blue bacilli in yellow mucus are available, but the chemistry for getting the yellow is not the same. John Kiernan Anatomy, UWO, London, Canada https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html = = = ________________________________ From: Gudrun Lang via Histonet Sent: 12 April 2020 03:38 To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Question concerning H. pylori staining Hi! I found this instruction for a Hp- stain, that sounds similiar to yours. They want the slides to be airdried after water-rinsing and before xylen. But the result should be blue bacteria, not purple. I would try to let the slides air-dry for about half a minute, then rinse very-very short in absolute ethanol (one dip). The colour should turn blue and some dye will be extracted to give a clearer result. Then fast into xylen, dip a few times, then coverslip. http://www.helicostat.com/helicostat_instructions.html I assume, that the periodic acid should render some mucins stainable with Alcian yellow, to give a more contrasted result. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Donnerstag, 9. April 2020 20:05 An: Histonet at lists.utsouthwestern.edu Betreff: Re: [Histonet] Question concerning H. pylori staining Michelle (where) asksi: >>We have a question about staining for H-Pylori Using Quick Stain (Periodic acid 1%, Alician Yellow, Sodium Metabisulfate, Toluidine Blue stock, Sodium Hydroxide) we notice what clearly looks like the H-Pylori purple stained clusters, but after dehydration in 100% alcohol the purple clusters seem to disappear. Should we just dehydrate using a slide oven instead of the alcohol? For how long at what temp? Could the alcohol be affecting the purple color making it too light to see?<< You identify Helicobacter pylori by its morphology - curved, angled, or "gull wing" bacilli. Is that what you're seeing? If you do this stain, you should know how it's interpreted. The Alcian yellow (correct spelling) method is needlessly complex. What does the periodic acid do? - A solution of toluidine blue (Diff-Quik 2 or a generic equivalent) suffices - don't use Diff-Quik 1 with it - dehydrate through alcohols rapidly. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From idimenstein at hotmail.com Mon Apr 13 12:53:38 2020 From: idimenstein at hotmail.com (Izak Dimenstein) Date: Mon, 13 Apr 2020 17:53:38 +0000 Subject: [Histonet] Histonet Digest, Vol 197, Issue 11 In-Reply-To: References: Message-ID: Please, go to my website grossing-technology.com. In the menu go to COVID-19. Open the PPE and beyond post. In the end of it is you. Any suggestions for corrections. Thank you. Sent from my iPad > On Apr 13, 2020, at 13:00, "histonet-request at lists.utsouthwestern.edu" wrote: > > ?Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Re: Question concerning H. pylori staining (jkiernan at uwo.ca) > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amurvosh at advancederm.net Mon Apr 13 15:07:42 2020 From: amurvosh at advancederm.net (Anne Murvosh) Date: Mon, 13 Apr 2020 20:07:42 +0000 Subject: [Histonet] Processor Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7CB49F0@Exchange.Advancederm.net> I have a back-up processor we keep filled for a back-up and if we have an extra run. However with lower volumes lately we aren't using it. I was just wondering about the paraffin in particularly. Does anyone know if you are not using it how long it can stay there (its heated) before changing out completely? Does it lose its stability? Thanks Anne Murvosh (HT) From deedeebrina at aol.com Tue Apr 14 08:54:17 2020 From: deedeebrina at aol.com (Dionee Neamand) Date: Tue, 14 Apr 2020 13:54:17 +0000 (UTC) Subject: [Histonet] Histonet Digest, Vol 197, Issue 8 In-Reply-To: References: Message-ID: <225266608.252011.1586872457936@mail.yahoo.com> Hello. I am looking for help with IHC staining. Does anyone have good negative control tissue for MMR stains? We need a minimum of 10 negative results per IHC stain for our validation. We have yet to find any. Any suggestions would be helpful. Thanks,Dee Dionee Neamand, HT (ASCP)Lead TechEvangelical Community HospitalLewisburg? PA? 17837 -----Original Message----- To: histonet at lists.utsouthwestern.edu Sent: Thu, Apr 9, 2020 1:00 pm Subject: Histonet Digest, Vol 197, Issue 8 Send Histonet mailing list submissions to ??? histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. Question concerning H-Pylori Staining (Ken M) ? 2. Is there anything I can do for you? (Pam Barker) ? 3. Competency questionnaires (Charles Riley) ---------------------------------------------------------------------- Message: 1 Date: Wed, 8 Apr 2020 19:50:09 +0000 From: Ken M To: Histo List Subject: [Histonet] Question concerning H-Pylori Staining Message-ID: ??? ??? Content-Type: text/plain; charset="iso-8859-1" Hi everyone!? We have a question about staining for H-Pylori Using Quick Stain (Periodic acid 1%, Alician Yellow, Sodium Metabisulfate,Toluidine Blue stock, Sodium Hydroxide) we notice what clearly? looks like the H-Pylori purple stained clusters, but after dehydration in 100% alcohol the purple clusters seem to disappear. Should we just dehydrate using a slide oven instead of the alcohol? For how long at what temp? Could the alcohol be affecting the purple color making it too light to see? Michelle ------------------------------ Message: 2 Date: Thu, 9 Apr 2020 11:15:47 -0400 From: "Pam Barker" To: "Histopeeps Histonet" Subject: [Histonet] Is there anything I can do for you? Message-ID: <008901d60e81$bf898880$3e9c9980$@earthlink.net> Content-Type: text/plain;??? charset="iso-8859-1" Hi Histonetters, I hope you and your loved ones are staying safe and healthy, and you can take time to enjoy Easter, Passover? and this season with your family. It seems that things are changing on an almost daily basis.? I have been monitoring social media - personal connections and histology related groups on Facebook and LinkedIN and have been staying in touch with many of my active candidates and customers. I have gotten a lot of questions about what is going on with the ?career? aspect of histology at this time. Here is a summary of what I have observed and experienced so far: Histotechs supporting ?non-essential surgeries? i.e. Derm and GI private practices: ? Some are experiencing cuts in hours and/or are using the time to catch up on backlogs and lab maintenance. ? Many are being furloughed with the expectation of being called back to work once ?the all clear is given?, ? Start dates for new employees are being postponed until further notice. Traveling Histotechs - Histotechs that are working as contractors nationwide: ? Most of the travel techs I have heard from have been grounded meaning sent home until further notice. ? They seem confident that they will be returning to their assignments as they are looking for prn positions for now. Hospital based histotechs: ? Some are being furloughed ? Some are having their hours reduced ? Some are job sharing meaning for example if they have a regular staff of 4 techs they take turns 2 at a time working for a week while the other 2 stay home ? Some are being used in other areas for example if they have experience in phlebotomy they are moving to that department. I can?t really speak to research, academic or pharmaceutical areas as I haven?t heard much from them.? (Hopefully that?s because they are busy working on the COVID-19 response.) What I glean from this and bear in mind this only an opinion on my part based on what I have heard anecdotally.? It seems that the best case scenario at this point is there was a shortage of histotechs before the pandemic and there will be just as great a need after the pandemic in other words we are just pressing pause. Just Remember? Things Will Get Better.? This Will End. & We Will Return To Our Normal Lives. In the meantime? Is there anything I can do for you? Were you actively looking for a new position before this crisis? Are you someone who is unemployed as opposed to furloughed? Are you feeling lonely or isolated and just need to hear a friendly voice? Call Me, Text Me, Email Me, Or Contact Me through Social Media. We are all in this together? Looking forward to the light at the end of the tunnel. Thanks-Pam Right Time, Right Place, Right Move with RELIA! *15 Years!* Celebrating 15 years of service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net? https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia? check out our latest opportunities at: http://www.jobvertise.com/members/relia1 #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! ------------------------------ Message: 3 Date: Thu, 9 Apr 2020 12:47:44 -0400 From: Charles Riley To: Histo List Subject: [Histonet] Competency questionnaires Message-ID: ??? Content-Type: text/plain; charset="UTF-8" Does anyone use a questionnaire for testing a techs competency either by itself or on top of practical application of the skills needed?? If so would you mind sharing with me your questions (I am looking for grossing, microtomy and troubleshooting, embedding, processing, and immunohistochemistry/special/H&E staining and troubleshooting. -- Charles Riley BS? HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 197, Issue 8 **************************************** From Timothy.Morken at ucsf.edu Tue Apr 14 12:31:43 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 14 Apr 2020 17:31:43 +0000 Subject: [Histonet] Who works in Hospitals? Message-ID: Something for others to know about https://www.linkedin.com/posts/activity-6653762952572788737-jqh8 Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From john.garratt at ciqc.ca Tue Apr 14 12:36:50 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Tue, 14 Apr 2020 17:36:50 +0000 Subject: [Histonet] Histonet Digest, Vol 197, Issue 8 In-Reply-To: <225266608.252011.1586872457936@mail.yahoo.com> References: <225266608.252011.1586872457936@mail.yahoo.com> Message-ID: Good morning, please take a look at one of the EQA reports (example in the link below) for MMR from the Canadian Pathology Quality Assurance programme. (www.cpqa.ca) . You cannot say MMR is positive or negative since it is confusing but say if there is Expression or Absence of Expression of a MMR protein. Fortunately tissues come with their own internal "positive" and "negative" controls which you can utilise. You could design a verification program around the presence or absence of a particular MMR protein should you wish. http://cpqa.ca/assessments/Run%2096%20MMR%20Summary.pdf Should you wish to receive our technically useful EQA reports in your mailbox email subscriptions at cpqa.ca putting SUBSCRIBE in the subject box. John www.cpqa.ca ??????? Original Message ??????? On Tuesday, April 14, 2020 6:54 AM, Dionee Neamand via Histonet wrote: > Hello. I am looking for help with IHC staining. Does anyone have good negative control tissue for MMR stains? We need a minimum of 10 negative results per IHC stain for our validation. We have yet to find any. Any suggestions would be helpful. > Thanks,Dee > Dionee Neamand, HT (ASCP)Lead TechEvangelical Community HospitalLewisburg? PA? 17837 > > -----Original Message----- > To: histonet at lists.utsouthwestern.edu > Sent: Thu, Apr 9, 2020 1:00 pm > Subject: Histonet Digest, Vol 197, Issue 8 > > Send Histonet mailing list submissions to > ??? histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > ??? histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > ??? histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > Today's Topics: > > ? 1. Question concerning H-Pylori Staining (Ken M) > ? 2. Is there anything I can do for you? (Pam Barker) > ? 3. Competency questionnaires (Charles Riley) > > > -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- > > Message: 1 > Date: Wed, 8 Apr 2020 19:50:09 +0000 > From: Ken M kdean70 at hotmail.com > To: Histo List histonet at lists.utsouthwestern.edu > Subject: [Histonet] Question concerning H-Pylori Staining > Message-ID: > ??? SN6PR06MB43343F3F0C62CBA60BDE54C8ADC00 at SN6PR06MB4334.namprd06.prod.outlook.com > > ??? > Content-Type: text/plain; charset="iso-8859-1" > > Hi everyone!? We have a question about staining for H-Pylori Using Quick Stain (Periodic acid 1%, Alician Yellow, Sodium Metabisulfate,Toluidine Blue stock, Sodium Hydroxide) we notice what clearly? looks like the H-Pylori purple stained clusters, but after dehydration in 100% alcohol the purple clusters seem to disappear. Should we just dehydrate using a slide oven instead of the alcohol? For how long at what temp? Could the alcohol be affecting the purple color making it too light to see? > > Michelle > > > ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- > > Message: 2 > Date: Thu, 9 Apr 2020 11:15:47 -0400 > From: "Pam Barker" relia1 at earthlink.net > To: "Histopeeps Histonet" histonet at lists.utsouthwestern.edu > Subject: [Histonet] Is there anything I can do for you? > Message-ID: 008901d60e81$bf898880$3e9c9980$@earthlink.net > > Content-Type: text/plain;??? charset="iso-8859-1" > > Hi Histonetters, > I hope you and your loved ones are staying safe and healthy, and you can > take time to enjoy Easter, Passover? and this season with your family. > It seems that things are changing on an almost daily basis.? > I have been monitoring social media - personal connections and histology > related groups on Facebook and LinkedIN and have been staying in touch with > many of my active candidates and customers. > I have gotten a lot of questions about what is going on with the ?career? > aspect of histology at this time. > Here is a summary of what I have observed and experienced so far: > > Histotechs supporting ?non-essential surgeries? i.e. Derm and GI private > practices: > ? Some are experiencing cuts in hours and/or are using the time to catch up > on backlogs and lab maintenance. > ? Many are being furloughed with the expectation of being called back to > work once ?the all clear is given?, > ? Start dates for new employees are being postponed until further notice. > > Traveling Histotechs - Histotechs that are working as contractors > nationwide: > ? Most of the travel techs I have heard from have been grounded meaning sent > home until further notice. > ? They seem confident that they will be returning to their assignments as > they are looking for prn positions for now. > > Hospital based histotechs: > ? Some are being furloughed > ? Some are having their hours reduced > ? Some are job sharing meaning for example if they have a regular staff of 4 > techs they take turns 2 at a time working for a week while the other 2 stay > home > ? Some are being used in other areas for example if they have experience in > phlebotomy they are moving to that department. > > I can?t really speak to research, academic or pharmaceutical areas as I > haven?t heard much from them.? (Hopefully that?s because they are busy > working on the COVID-19 response.) > > What I glean from this and bear in mind this only an opinion on my part > based on what I have heard anecdotally.? It seems that the best case > scenario at this point is there was a shortage of histotechs before the > pandemic and there will be just as great a need after the pandemic in other > words we are just pressing pause. > > Just Remember? > Things Will Get Better.? > This Will End. > & > We Will Return To Our Normal Lives. > > In the meantime? > Is there anything I can do for you? > Were you actively looking for a new position before this crisis? > Are you someone who is unemployed as opposed to furloughed? > Are you feeling lonely or isolated and just need to hear a friendly voice? > Call Me, > Text Me, > Email Me, > Or Contact Me through Social Media. > > We are all in this together? > > Looking forward to the light at the end of the tunnel. > > Thanks-Pam > > Right Time, Right Place, Right Move with RELIA! > 15 Years! > Celebrating 15 years of service exclusively to the Histology Community! > > Thank You! > ?Pam M. Barker? > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell:???? (407)353-5070 > FAX:???? (407)678-2788 > E-mail: relia1 at earthlink.net? > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > www.facebook.com/PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia? > check out our latest opportunities at: > http://www.jobvertise.com/members/relia1 > #jobs4myhistopeeps > #ilovemyhistopeeps > #histopeeps > Follow my hashtags and make your day great and your career greater!! > > > --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- > > Message: 3 > Date: Thu, 9 Apr 2020 12:47:44 -0400 > From: Charles Riley criley at dpspa.com > To: Histo List histonet at lists.utsouthwestern.edu > Subject: [Histonet] Competency questionnaires > Message-ID: > ??? CAAQhB10D2e=CMBZD6nqGcrX19sWQneBOnYZy1-ijTfb7yu-XOA at mail.gmail.com > > Content-Type: text/plain; charset="UTF-8" > > Does anyone use a questionnaire for testing a techs competency either by > itself or on top of practical application of the skills needed?? If so > would you mind sharing with me your questions (I am looking for grossing, > microtomy and troubleshooting, embedding, processing, and > immunohistochemistry/special/H&E staining and troubleshooting. > > ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- > > Charles Riley BS? HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > > > ----------------------------------------------------------------------- > > Subject: Digest Footer > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------------------------------------------------------------------------------------------ > > End of Histonet Digest, Vol 197, Issue 8 > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From John.Shelton at UTSouthwestern.edu Tue Apr 14 14:49:36 2020 From: John.Shelton at UTSouthwestern.edu (John Shelton) Date: Tue, 14 Apr 2020 19:49:36 +0000 Subject: [Histonet] Fw: QUESTION FOR LIST In-Reply-To: References: Message-ID: <1586893775553.54141@UTSouthwestern.edu> Sure thing Terri... posting John M Shelton Core Operations Manager Histonet List Owner & Moderator UT Southwestern Histo Pathology Core 5323 Harry Hines Blvd Dallas, Texas 75390-8573 (214)648-1451 Visit the Core's website at: http://www.utsouthwestern.edu/labs/histo-pathology/ Visit Histonet Archives at: http://www.histosearch.com/histonet.html ? ________________________________ From: Terri Clarke Sent: Tuesday, April 14, 2020 2:28 PM To: histonet-owner at lists.utsouthwestern.edu Subject: QUESTION FOR LIST EXTERNAL MAIL Can you please post this question to the list? thanks We have a remote site that now uses digital pathology to read the frozen section slides, the question is what should the TAT be for those cases? Typically it is 20 minutes for a frozen section when the Pathologist is on site. Are there any other sites out there that are using digital pathology for frozen sections? thanks Terri Clarke Manager Anatomic Pathology Ascension Wisconsin Laboratories and Columbia-St. Mary's T: 414-447-2424 (at St. Joe's) T: 414-585-1413 (at CSM) T: 262-687-6609 ( at All Saints) F: 414-447-2272 (at St. Joseph"s terri.clarke at ascension.org CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. CAUTION: This email originated from outside UTSW. Please be cautious of links or attachments, and validate the sender's email address before replying. ________________________________ UT Southwestern Medical Center The future of medicine, today. From jamie at watson-home.com Wed Apr 15 18:44:55 2020 From: jamie at watson-home.com (Jamie Watson) Date: Wed, 15 Apr 2020 23:44:55 +0000 Subject: [Histonet] Microtome at home Message-ID: Hello all, Our pathologist has come up with the idea of sending a microtome and waterbath home to someone that cannot come to work due to COVID 19. We are a research lab and work with mouse and rat tissue. Does anyone know of any issues with doing this? I have never heard of anyone cutting slides at home other than someone with a private business. Thank you. Jamie From raestask at grics.net Wed Apr 15 18:50:37 2020 From: raestask at grics.net (raestask) Date: Wed, 15 Apr 2020 18:50:37 -0500 Subject: [Histonet] Microtome at home In-Reply-To: Message-ID: I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone -------- Original message --------From: Jamie Watson via Histonet Date: 4/15/20 6:44 PM (GMT-06:00) To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello all,Our pathologist has come up with the idea of sending a microtome and waterbath home to someone that cannot come to work due to COVID 19.? We are a research lab and work with mouse and rat tissue.? Does anyone know of any issues with doing this?? I have never heard of anyone cutting slides at home other than someone with a private business.Thank you.Jamie_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From scrochiere at nedlc.com Thu Apr 16 05:36:35 2020 From: scrochiere at nedlc.com (Steven Crochiere) Date: Thu, 16 Apr 2020 06:36:35 -0400 Subject: [Histonet] FW: Microtome at home In-Reply-To: References: Message-ID: <000201d613da$e7a18990$b6e49cb0$@nedlc.com> Jaime, I don't see a problem with a research setting. If it was patient care, CLIA would need to inspect the set up in the person home. The same goes for our pathologists who read slide at home. Steve -----Original Message----- From: raestask via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 15, 2020 7:51 PM To: Jamie Watson ; Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome at home I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone -------- Original message --------From: Jamie Watson via Histonet Date: 4/15/20 6:44 PM (GMT-06:00) To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello all,Our pathologist has come up with the idea of sending a microtome and waterbath home to someone that cannot come to work due to COVID 19. We are a research lab and work with mouse and rat tissue. Does anyone know of any issues with doing this? I have never heard of anyone cutting slides at home other than someone with a private business.Thank you.Jamie_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera at msu.edu Thu Apr 16 07:11:20 2020 From: portera at msu.edu (Porter, Amy) Date: Thu, 16 Apr 2020 12:11:20 +0000 Subject: [Histonet] FW: Microtome at home In-Reply-To: <000201d613da$e7a18990$b6e49cb0$@nedlc.com> References: , <000201d613da$e7a18990$b6e49cb0$@nedlc.com> Message-ID: Make sure of insurance coverage and safety for the employee and that they are covered in case of injury - are they still clocking in and out in some fashion..... just thinking in a bigger box. ________________________________ From: Steven Crochiere via Histonet Sent: Thursday, April 16, 2020 6:36 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] FW: Microtome at home Jaime, I don't see a problem with a research setting. If it was patient care, CLIA would need to inspect the set up in the person home. The same goes for our pathologists who read slide at home. Steve -----Original Message----- From: raestask via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 15, 2020 7:51 PM To: Jamie Watson ; Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome at home I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone -------- Original message --------From: Jamie Watson via Histonet Date: 4/15/20 6:44 PM (GMT-06:00) To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello all,Our pathologist has come up with the idea of sending a microtome and waterbath home to someone that cannot come to work due to COVID 19. We are a research lab and work with mouse and rat tissue. Does anyone know of any issues with doing this? I have never heard of anyone cutting slides at home other than someone with a private business.Thank you.Jamie_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ From saby_joseph_a at yahoo.com Thu Apr 16 09:03:19 2020 From: saby_joseph_a at yahoo.com (Joseph Saby) Date: Thu, 16 Apr 2020 14:03:19 +0000 (UTC) Subject: [Histonet] FW: Microtome at home In-Reply-To: References: <000201d613da$e7a18990$b6e49cb0$@nedlc.com> Message-ID: <897188987.1575775.1587045799142@mail.yahoo.com> You will need to make sure all pertinent SOPs and EOPs are followed, as well as all safety guidelines/protocols. Just because it is not human tissue doesn't mean that it can't have its share of nasties.? Joe Saby Sent from Yahoo Mail on Android On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy via Histonet wrote: Make sure of insurance coverage and safety for the employee and that they are covered in case of injury - are they still clocking in and out in some fashion..... just thinking in a bigger box. ________________________________ From: Steven Crochiere via Histonet Sent: Thursday, April 16, 2020 6:36 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] FW: Microtome at home Jaime, I don't see a problem with a research setting. If it was patient care, CLIA would need to inspect the set up in the person home. The same goes for our pathologists who read slide at home. Steve -----Original Message----- From: raestask via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 15, 2020 7:51 PM To: Jamie Watson ; Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome at home I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone -------- Original message --------From: Jamie Watson via Histonet Date: 4/15/20? 6:44 PM? (GMT-06:00) To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello all,Our pathologist has come up with the idea of sending a microtome and waterbath home to someone that cannot come to work due to COVID 19.? We are a research lab and work with mouse and rat tissue.? Does anyone know of any issues with doing this?? I have never heard of anyone cutting slides at home other than someone with a private business.Thank you.Jamie_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From saby_joseph_a at yahoo.com Thu Apr 16 09:03:19 2020 From: saby_joseph_a at yahoo.com (Joseph Saby) Date: Thu, 16 Apr 2020 14:03:19 +0000 (UTC) Subject: [Histonet] FW: Microtome at home In-Reply-To: References: <000201d613da$e7a18990$b6e49cb0$@nedlc.com> Message-ID: <897188987.1575775.1587045799142@mail.yahoo.com> You will need to make sure all pertinent SOPs and EOPs are followed, as well as all safety guidelines/protocols. Just because it is not human tissue doesn't mean that it can't have its share of nasties.? Joe Saby Sent from Yahoo Mail on Android On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy via Histonet wrote: Make sure of insurance coverage and safety for the employee and that they are covered in case of injury - are they still clocking in and out in some fashion..... just thinking in a bigger box. ________________________________ From: Steven Crochiere via Histonet Sent: Thursday, April 16, 2020 6:36 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] FW: Microtome at home Jaime, I don't see a problem with a research setting. If it was patient care, CLIA would need to inspect the set up in the person home. The same goes for our pathologists who read slide at home. Steve -----Original Message----- From: raestask via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 15, 2020 7:51 PM To: Jamie Watson ; Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome at home I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone -------- Original message --------From: Jamie Watson via Histonet Date: 4/15/20? 6:44 PM? (GMT-06:00) To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello all,Our pathologist has come up with the idea of sending a microtome and waterbath home to someone that cannot come to work due to COVID 19.? We are a research lab and work with mouse and rat tissue.? Does anyone know of any issues with doing this?? I have never heard of anyone cutting slides at home other than someone with a private business.Thank you.Jamie_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at dpspa.com Thu Apr 16 12:38:00 2020 From: criley at dpspa.com (Charles Riley) Date: Thu, 16 Apr 2020 13:38:00 -0400 Subject: [Histonet] CAP proficiency testing Message-ID: Does anyone use another vendor besides CAP to perform their PT requirements? If so, who do you use and how do you record it for inspection purposes? I am looking for a PD-L1 PT specifically but will take any other options as well as we are trying to help our budget. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From john.garratt at ciqc.ca Thu Apr 16 13:44:07 2020 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 16 Apr 2020 18:44:07 +0000 Subject: [Histonet] CAP proficiency testing In-Reply-To: References: Message-ID: The Canadian Pathology Quality Assurance programme runs IHC and FISH EQA internationally. We have performed PD-L1 EQA previously and we will be running a challenge January 2021. Previous EQA reports are available on line. Our website is www.cpqa.ca. We work with regularity authorities to provide compliance information when requested by the participant. John On Thu, Apr 16, 2020 at 10:38 AM, Charles Riley via Histonet wrote: > Does anyone use another vendor besides CAP to perform their PT > requirements? > > If so, who do you use and how do you record it for inspection purposes? > > I am looking for a PD-L1 PT specifically but will take any other options as > well as we are trying to help our budget. > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdean70 at hotmail.com Thu Apr 16 15:06:13 2020 From: kdean70 at hotmail.com (Ken M) Date: Thu, 16 Apr 2020 20:06:13 +0000 Subject: [Histonet] Recommended thickness of Amyloid sections Message-ID: Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience? Ken From paula at excaliburpathology.com Thu Apr 16 15:24:22 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Thu, 16 Apr 2020 20:24:22 +0000 (UTC) Subject: [Histonet] Recommended thickness of Amyloid sections In-Reply-To: References: Message-ID: <1350282959.1196145.1587068662405@mail.yahoo.com> As this is my 41 year of being a registered HT, I was taught that slides for amyloid are to be cut at 8-10?m. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, April 16, 2020, 03:18:44 PM CDT, Ken M via Histonet wrote: Hello All.? We have always cut all of our histology control slides at 5m.? We were told today that it is common practice to cut Amyloid at 8m?? Is this your experience? Ken _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From raestask at grics.net Thu Apr 16 15:29:50 2020 From: raestask at grics.net (raestask) Date: Thu, 16 Apr 2020 15:29:50 -0500 Subject: [Histonet] Recommended thickness of Amyloid sections In-Reply-To: <1350282959.1196145.1587068662405@mail.yahoo.com> Message-ID: <11.02.21087.240C89E5@smtp04.onyx.dfw.sync.lan> Should also be cut on the day they are to be stained.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone -------- Original message --------From: Paula Keene Pierce via Histonet Date: 4/16/20 3:24 PM (GMT-06:00) To: histonet at lists.utsouthwestern.edu, Ken M Subject: Re: [Histonet] Recommended thickness of Amyloid sections As this is my 41 year of being a registered HT, I was taught that slides for amyloid are to be cut at 8-10?m.Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.comA sharp knife is nothing without a sharp eye. - Klingon Proverb ??? On Thursday, April 16, 2020, 03:18:44 PM CDT, Ken M via Histonet wrote:? Hello All.? We have always cut all of our histology control slides at 5m.? We were told today that it is common practice to cut Amyloid at 8m?? Is this your experience?Ken_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet? _______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From terrie.dalton at icloud.com Thu Apr 16 15:32:50 2020 From: terrie.dalton at icloud.com (Theresa Dalton) Date: Thu, 16 Apr 2020 16:32:50 -0400 Subject: [Histonet] Recommended thickness of Amyloid sections In-Reply-To: References: Message-ID: <768E5F77-13C2-49B6-AF5D-FE6E3679144A@icloud.com> I work at a Dermatology lab and our protocol for amyloid is 8-10 microns. Sent from my iPhone > On Apr 16, 2020, at 4:19 PM, Ken M via Histonet wrote: > > ?Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience? > > Ken > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Thu Apr 16 15:38:58 2020 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 16 Apr 2020 20:38:58 +0000 Subject: [Histonet] Recommended thickness of Amyloid sections In-Reply-To: <1350282959.1196145.1587068662405@mail.yahoo.com> References: , <1350282959.1196145.1587068662405@mail.yahoo.com> Message-ID: <1587069538459.24350@health.nsw.gov.au> I agree, Slightly thicker makes the polarisation easier to see (personal experience). I would love to see a study comparing section thickness Vs polarisation characteristics. Anyone interested? (being a kids hospital we rarely see amyloidosis) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Paula Keene Pierce via Histonet Sent: Friday, 17 April 2020 06:24 To: histonet at lists.utsouthwestern.edu; Ken M Subject: Re: [Histonet] Recommended thickness of Amyloid sections As this is my 41 year of being a registered HT, I was taught that slides for amyloid are to be cut at 8-10?m. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, April 16, 2020, 03:18:44 PM CDT, Ken M via Histonet wrote: Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience? Ken _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From Timothy.Morken at ucsf.edu Thu Apr 16 15:41:21 2020 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 16 Apr 2020 20:41:21 +0000 Subject: [Histonet] Recommended thickness of Amyloid sections In-Reply-To: References: Message-ID: Ken, yes, 8 to 10 um. The extra thickness make the bi-refringence under polarized light brighter. Also the deposits can be variable so even with the light microscope the reddish deposits will stain stronger with thicker sections. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Ken M via Histonet Sent: Thursday, April 16, 2020 1:06 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Recommended thickness of Amyloid sections Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience? Ken _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=zu56n7jP8NfTVcr03S3ER1yqzKYomhe2VIC5gz5CiHI&s=OTIHpP1NshAzBBAOzuP2Ljiz166ODsMhL_CDtbvQJgs&e= From pruegghm at hotmail.com Thu Apr 16 16:45:31 2020 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Thu, 16 Apr 2020 21:45:31 +0000 Subject: [Histonet] Microtome at home In-Reply-To: References: Message-ID: I have done it, but you are right, I had my own private business, not sure why it would be a problem, especially for research. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Jamie Watson Sent: Wednesday, April 15, 2020 5:44 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello all, Our pathologist has come up with the idea of sending a microtome and waterbath home to someone that cannot come to work due to COVID 19. We are a research lab and work with mouse and rat tissue. Does anyone know of any issues with doing this? I have never heard of anyone cutting slides at home other than someone with a private business. Thank you. Jamie From pruegghm at hotmail.com Thu Apr 16 16:48:51 2020 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Thu, 16 Apr 2020 21:48:51 +0000 Subject: [Histonet] FW: Microtome at home In-Reply-To: <897188987.1575775.1587045799142@mail.yahoo.com> References: <000201d613da$e7a18990$b6e49cb0$@nedlc.com> , <897188987.1575775.1587045799142@mail.yahoo.com> Message-ID: I agree with this point and as far as clocking in and out, I would think you could work out something like getting paid piece mill, perhaps charge per slide or block cut, that way you could do it on your own time and not have to clock in. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Joseph Saby Sent: Thursday, April 16, 2020 8:03 AM To: Porter, Amy ; Porter, Amy via Histonet ; histonet at lists.utsouthwestern.edu ; Steven Crochiere Subject: Re: [Histonet] FW: Microtome at home You will need to make sure all pertinent SOPs and EOPs are followed, as well as all safety guidelines/protocols. Just because it is not human tissue doesn't mean that it can't have its share of nasties. Joe Saby Sent from Yahoo Mail on Android On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy via Histonet wrote: Make sure of insurance coverage and safety for the employee and that they are covered in case of injury - are they still clocking in and out in some fashion..... just thinking in a bigger box. ________________________________ From: Steven Crochiere via Histonet Sent: Thursday, April 16, 2020 6:36 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] FW: Microtome at home Jaime, I don't see a problem with a research setting. If it was patient care, CLIA would need to inspect the set up in the person home. The same goes for our pathologists who read slide at home. Steve -----Original Message----- From: raestask via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, April 15, 2020 7:51 PM To: Jamie Watson ; Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Microtome at home I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone -------- Original message --------From: Jamie Watson via Histonet Date: 4/15/20 6:44 PM (GMT-06:00) To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello all,Our pathologist has come up with the idea of sending a microtome and waterbath home to someone that cannot come to work due to COVID 19. We are a research lab and work with mouse and rat tissue. Does anyone know of any issues with doing this? I have never heard of anyone cutting slides at home other than someone with a private business.Thank you.Jamie_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From roxjean3 at gmail.com Thu Apr 16 17:20:39 2020 From: roxjean3 at gmail.com (Roxana Robinson) Date: Thu, 16 Apr 2020 17:20:39 -0500 Subject: [Histonet] Recommended thickness of Amyloid sections In-Reply-To: <11.02.21087.240C89E5@smtp04.onyx.dfw.sync.lan> References: <11.02.21087.240C89E5@smtp04.onyx.dfw.sync.lan> Message-ID: <69C27ED8-6D7D-4B67-B4BB-84167D46FCBC@gmail.com> Agree. 8-10 microns cut just prior to staining. Roxana Robinson > On Apr 16, 2020, at 3:50 PM, raestask via Histonet wrote: > > ?Should also be cut on the day they are to be stained.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone > -------- Original message --------From: Paula Keene Pierce via Histonet Date: 4/16/20 3:24 PM (GMT-06:00) To: histonet at lists.utsouthwestern.edu, Ken M Subject: Re: [Histonet] Recommended thickness of Amyloid sections As this is my 41 year of being a registered HT, I was taught that slides for amyloid are to be cut at 8-10?m.Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.comA sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, April 16, 2020, 03:18:44 PM CDT, Ken M via Histonet wrote: Hello All. We have always cut all of our histology control slides at 5m. We were told today that it is common practice to cut Amyloid at 8m? Is this your experience?Ken_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From roxjean3 at gmail.com Thu Apr 16 17:28:34 2020 From: roxjean3 at gmail.com (Roxana Robinson) Date: Thu, 16 Apr 2020 17:28:34 -0500 Subject: [Histonet] FW: Microtome at home In-Reply-To: References: Message-ID: I do not agree with this in our current situation or actually any situation. There are quidelines in place with CLIA, OHSA and CAP for protecting not only the patient but also the employee. Whether research or not. Roxana Robinson > On Apr 16, 2020, at 4:58 PM, Patsy Ruegg via Histonet wrote: > > ?I agree with this point and as far as clocking in and out, I would think you could work out something like getting paid piece mill, perhaps charge per slide or block cut, that way you could do it on your own time and not have to clock in. > > > Patsy Ruegg, HT(ASCP)QIHC > Ruegg IHC Consulting > 40864 E Arkansas Ave > Bennett, CO 80102 > H 303-644-4538 > C 720-281-5406 > pruegghm at hotmail.com > > > ________________________________ > From: Joseph Saby > Sent: Thursday, April 16, 2020 8:03 AM > To: Porter, Amy ; Porter, Amy via Histonet ; histonet at lists.utsouthwestern.edu ; Steven Crochiere > Subject: Re: [Histonet] FW: Microtome at home > > > You will need to make sure all pertinent SOPs and EOPs are followed, as well as all safety guidelines/protocols. Just because it is not human tissue doesn't mean that it can't have its share of nasties. > Joe Saby > > Sent from Yahoo Mail on Android > > On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy via Histonet wrote: Make sure of insurance coverage and safety for the employee and that they are covered in case of injury - are they still clocking in and out in some fashion..... just thinking in a bigger box. > > ________________________________ > From: Steven Crochiere via Histonet > Sent: Thursday, April 16, 2020 6:36 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] FW: Microtome at home > > Jaime, > > I don't see a problem with a research setting. If it was patient care, CLIA would need to inspect the set up in the person home. The same goes for our pathologists who read slide at home. > > Steve > > -----Original Message----- > From: raestask via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, April 15, 2020 7:51 PM > To: Jamie Watson ; Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Microtome at home > > I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent from my Verizon, Samsung Galaxy smartphone > -------- Original message --------From: Jamie Watson via Histonet Date: 4/15/20 6:44 PM (GMT-06:00) To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Microtome at home Hello all,Our pathologist has come up with the idea of sending a microtome and waterbath home to someone that cannot come to work due to COVID 19. We are a research lab and work with mouse and rat tissue. Does anyone know of any issues with doing this? I have never heard of anyone cutting slides at home other than someone with a private business.Thank you.Jamie_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From marktarango at gmail.com Thu Apr 16 19:13:12 2020 From: marktarango at gmail.com (Mark Tarango) Date: Thu, 16 Apr 2020 17:13:12 -0700 Subject: [Histonet] FW: Microtome at home In-Reply-To: References: Message-ID: I had heard that CLIA was relaxing things and is not requiring a new # to work from home right now. Best to check on the regulatory but FFPE isn't typically infectious. The ideal spot would in the garage and not the kitchen though. On Thu, Apr 16, 2020 at 3:47 PM Roxana Robinson via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I do not agree with this in our current situation or actually any > situation. > There are quidelines in place with CLIA, OHSA and CAP for protecting not > only the patient but also the employee. Whether research or not. > > > Roxana Robinson > > > On Apr 16, 2020, at 4:58 PM, Patsy Ruegg via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > > ?I agree with this point and as far as clocking in and out, I would > think you could work out something like getting paid piece mill, perhaps > charge per slide or block cut, that way you could do it on your own time > and not have to clock in. > > > > > > Patsy Ruegg, HT(ASCP)QIHC > > Ruegg IHC Consulting > > 40864 E Arkansas Ave > > Bennett, CO 80102 > > H 303-644-4538 > > C 720-281-5406 > > pruegghm at hotmail.com > > > > > > ________________________________ > > From: Joseph Saby > > Sent: Thursday, April 16, 2020 8:03 AM > > To: Porter, Amy ; Porter, Amy via Histonet < > histonet at lists.utsouthwestern.edu>; histonet at lists.utsouthwestern.edu < > histonet at lists.utsouthwestern.edu>; Steven Crochiere > > > Subject: Re: [Histonet] FW: Microtome at home > > > > > > You will need to make sure all pertinent SOPs and EOPs are followed, as > well as all safety guidelines/protocols. Just because it is not human > tissue doesn't mean that it can't have its share of nasties. > > Joe Saby > > > > Sent from Yahoo Mail on Android > > > > On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy via Histonet< > histonet at lists.utsouthwestern.edu> wrote: Make sure of insurance > coverage and safety for the employee and that they are covered in case of > injury - are they still clocking in and out in some fashion..... just > thinking in a bigger box. > > > > ________________________________ > > From: Steven Crochiere via Histonet > > Sent: Thursday, April 16, 2020 6:36 AM > > To: histonet at lists.utsouthwestern.edu > > > Subject: [Histonet] FW: Microtome at home > > > > Jaime, > > > > I don't see a problem with a research setting. If it was patient care, > CLIA would need to inspect the set up in the person home. The same goes for > our pathologists who read slide at home. > > > > Steve > > > > -----Original Message----- > > From: raestask via Histonet [mailto:histonet at lists.utsouthwestern.edu] > > Sent: Wednesday, April 15, 2020 7:51 PM > > To: Jamie Watson ; > Histonet at lists.utsouthwestern.edu > > Subject: Re: [Histonet] Microtome at home > > > > I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent > from my Verizon, Samsung Galaxy smartphone > > -------- Original message --------From: Jamie Watson via Histonet < > histonet at lists.utsouthwestern.edu> Date: 4/15/20 6:44 PM (GMT-06:00) > To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Microtome at > home Hello all,Our pathologist has come up with the idea of sending a > microtome and waterbath home to someone that cannot come to work due to > COVID 19. We are a research lab and work with mouse and rat tissue. Does > anyone know of any issues with doing this? I have never heard of anyone > cutting slides at home other than someone with a private business.Thank > you.Jamie_______________________________________________Histonet mailing > listHistonet at lists.utsouthwestern.eduhttp:// > lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ewj at pigs.ag Thu Apr 16 20:18:47 2020 From: ewj at pigs.ag (E. Wayne Johnson) Date: Fri, 17 Apr 2020 09:18:47 +0800 Subject: [Histonet] FW: Microtome at home In-Reply-To: References: Message-ID: "Taking control of the situation is key." It's very interesting to me that most of the responses have to do with institutionalized bureaucratic ideas (safety, insurance, liability, regulations) rather than how to get things done. I was thinking about how Virchow and Henle and other pioneers would have been able to get anything done in the face of such reverence for the stultifying bureaucracy that is the enemy of effectiveness. Afraid to decide, no action is taken. /"Auftragstaktik/ can be seen as a doctrine within which formal rules can be selectively suspended in order to overcome "friction"." E. Wayne Johnson DVM Enable AgTech Beijing Mark Tarango via Histonet wrote: > I had heard that CLIA was relaxing things and is not requiring a new # to > work from home right now. Best to check on the regulatory but FFPE isn't > typically infectious. The ideal spot would in the garage and not the > kitchen though. > > On Thu, Apr 16, 2020 at 3:47 PM Roxana Robinson via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> I do not agree with this in our current situation or actually any >> situation. >> There are quidelines in place with CLIA, OHSA and CAP for protecting not >> only the patient but also the employee. Whether research or not. >> >> >> Roxana Robinson >> >>> On Apr 16, 2020, at 4:58 PM, Patsy Ruegg via Histonet < >> histonet at lists.utsouthwestern.edu> wrote: >>> ?I agree with this point and as far as clocking in and out, I would >> think you could work out something like getting paid piece mill, perhaps >> charge per slide or block cut, that way you could do it on your own time >> and not have to clock in. >>> >>> Patsy Ruegg, HT(ASCP)QIHC >>> Ruegg IHC Consulting >>> 40864 E Arkansas Ave >>> Bennett, CO 80102 >>> H 303-644-4538 >>> C 720-281-5406 >>> pruegghm at hotmail.com >>> >>> >>> ________________________________ >>> From: Joseph Saby >>> Sent: Thursday, April 16, 2020 8:03 AM >>> To: Porter, Amy ; Porter, Amy via Histonet < >> histonet at lists.utsouthwestern.edu>; histonet at lists.utsouthwestern.edu < >> histonet at lists.utsouthwestern.edu>; Steven Crochiere >> Subject: Re: [Histonet] FW: Microtome at home >>> >>> >>> You will need to make sure all pertinent SOPs and EOPs are followed, as >> well as all safety guidelines/protocols. Just because it is not human >> tissue doesn't mean that it can't have its share of nasties. >>> Joe Saby >>> >>> Sent from Yahoo Mail on Android >>> >>> On Thu, Apr 16, 2020 at 8:21 AM, Porter, Amy via Histonet< >> histonet at lists.utsouthwestern.edu> wrote: Make sure of insurance >> coverage and safety for the employee and that they are covered in case of >> injury - are they still clocking in and out in some fashion..... just >> thinking in a bigger box. >>> ________________________________ >>> From: Steven Crochiere via Histonet >>> Sent: Thursday, April 16, 2020 6:36 AM >>> To: histonet at lists.utsouthwestern.edu >> >>> Subject: [Histonet] FW: Microtome at home >>> >>> Jaime, >>> >>> I don't see a problem with a research setting. If it was patient care, >> CLIA would need to inspect the set up in the person home. The same goes for >> our pathologists who read slide at home. >>> Steve >>> >>> -----Original Message----- >>> From: raestask via Histonet [mailto:histonet at lists.utsouthwestern.edu] >>> Sent: Wednesday, April 15, 2020 7:51 PM >>> To: Jamie Watson ; >> Histonet at lists.utsouthwestern.edu >>> Subject: Re: [Histonet] Microtome at home >>> >>> I wouldn't think there would be any problem.Rae Staskiewicz HT(ASCP)Sent >> from my Verizon, Samsung Galaxy smartphone >>> -------- Original message --------From: Jamie Watson via Histonet < >> histonet at lists.utsouthwestern.edu> Date: 4/15/20 6:44 PM (GMT-06:00) >> To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Microtome at >> home Hello all,Our pathologist has come up with the idea of sending a >> microtome and waterbath home to someone that cannot come to work due to >> COVID 19. We are a research lab and work with mouse and rat tissue. Does >> anyone know of any issues with doing this? I have never heard of anyone >> cutting slides at home other than someone with a private business.Thank >> you.Jamie_______________________________________________Histonet mailing >> listHistonet at lists.utsouthwestern.eduhttp:// >> lists.utsouthwestern.edu/mailman/listinfo/histonet >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> >> https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> >> https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!nwH0INyWP6lMeJ_Devv2eaelHE25_36kcLQnnnBFaO46Y8_BkxEnT0U_DaplXA$ >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Fri Apr 17 12:46:20 2020 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 17 Apr 2020 13:46:20 -0400 Subject: [Histonet] Recommended thickness of Amyloid sections In-Reply-To: References: Message-ID: In response to several questions and responses about amyloid staining: I?ve always understood that sections for amyloid should be cut at 8 ?m, but could never get a histotech to cut them that thick. I recall that unstained paraffin sections on the slide are usable for about a month after cutting. That limitation precludes buying commercial controls. Most (though not all) medullary carcinomas of the thyroid contain some amyloid, and that?s about the only control you can get for yourself, and it?s a rather rare tumor, and not easily recovered from a lab?s old records. Every time somebody posts on amyloid on Histonet or elsewhere I?ve asked this question, and never got the slightest reply: Amyloid (of the AL type, I suppose) can rather easily be induced in experimental animals (by injection of casein, for example). Does any pathology service use animal material for amyloid controls? Is it commercially available? In 2002 Dick Dapson at Anatech introduced ?Amyloid Red? (Direct Red 72, C.I. 29200) as a substitute for Congo red. I just checked the Web site and Anatech still offers it. Does anyone have any experience with it? Bob Richmond Samurai Pathologist Maryville TN From carl.hobbs at kcl.ac.uk Fri Apr 17 12:54:50 2020 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 17 Apr 2020 17:54:50 +0000 Subject: [Histonet] Recommended thickness of Amyloid sections Message-ID: Depends on what you want to do: positivity or maximise signal? I have FS and Pwax images up in Histonet image archive if you care to have a look Best wishes to all Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From redrose297 at gmail.com Sun Apr 19 04:36:43 2020 From: redrose297 at gmail.com (warda hassan) Date: Sun, 19 Apr 2020 13:36:43 +0400 Subject: [Histonet] Spectra Message-ID: Good Morning to all Thanks in advance to all who would help with their feedback on the automation of H/E staining, Initially we have installed Spectra-Leica for H/E The feedback from pathologist is the stain is bit on bluish and eosin is weak We did altered and reached to 2 min of Hematoxylin and 11 min Eosin But unfortunately the stain consistency is not stable as after every 2-3 racks level of reagents reduces so we top up as we do the intensity of it differs Second issue is Turn around time, each rack takes 45mins, so tried adding up new protocol as copy but the machine keeps a gap of 12mins in picking up the slides for staining ! If anyone of you have experienced with Leica automation Please your feedback on above would be of a great help Thank you so much From LRaff at uropartners.com Mon Apr 20 06:20:34 2020 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 20 Apr 2020 11:20:34 +0000 Subject: [Histonet] A month since the last group of COVID-19 Haiku. How is everyone hanging in? Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF12A2CFDC@COLOEXCH01.uropartners.local> http://www.chicagonow.com/downsize-maybe/2020/04/haiku-2/ Lester J. Raff, MD MBA FCAP Laboratory Director UroPartners LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 Telephone 708-486-0076 Fax 708-492-0203 From Richard.Cartun at hhchealth.org Mon Apr 20 15:50:49 2020 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 20 Apr 2020 20:50:49 +0000 Subject: [Histonet] Formalin fixation for COVID-19 positive tissues ..... Message-ID: <9215BD4B0BA1B44D962A71C758B68D2ED45B68A1@HHCEXCHMB03.hhcsystem.org> How long are you fixing surgical tissue specimens from COVID-19 positive patient's before tissue processing? I know that the CDC is recommending "72" hours for autopsy tissues, but, to me, that seems excessive for surgical pathology specimens. Any information that you can share on this subject would be appreciated. Thank you and stay safe. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From tony.henwood at health.nsw.gov.au Mon Apr 20 18:33:24 2020 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon, 20 Apr 2020 23:33:24 +0000 Subject: [Histonet] Formalin fixation for COVID-19 positive tissues ..... In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2ED45B68A1@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2ED45B68A1@HHCEXCHMB03.hhcsystem.org> Message-ID: <0ea113a98f4c499cb01639d980d3ef62@SVDCMBX-MEX024.nswhealth.net> Hi Richard, It will depend on the size of the tissue and the source. Lung tissue is the major concern. Other tissues not affected as much (based on the burgeoning literature on Covid-19). Routine fixation time are applicable, remembering that the alcohols and heated wax will also inactivate the virus (triple whammy). I suppose that I better add a reference: https://www.tandfonline.com/doi/full/10.1080/01478885.2020.1734718 -----Original Message----- From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, 21 April 2020 6:51 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Formalin fixation for COVID-19 positive tissues ..... How long are you fixing surgical tissue specimens from COVID-19 positive patient's before tissue processing? I know that the CDC is recommending "72" hours for autopsy tissues, but, to me, that seems excessive for surgical pathology specimens. Any information that you can share on this subject would be appreciated. Thank you and stay safe. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From ewj at pigs.ag Tue Apr 21 01:07:04 2020 From: ewj at pigs.ag (E. Wayne Johnson) Date: Tue, 21 Apr 2020 14:07:04 +0800 Subject: [Histonet] Formalin fixation for COVID-19 positive tissues ..... In-Reply-To: <0ea113a98f4c499cb01639d980d3ef62@SVDCMBX-MEX024.nswhealth.net> References: <9215BD4B0BA1B44D962A71C758B68D2ED45B68A1@HHCEXCHMB03.hhcsystem.org> <0ea113a98f4c499cb01639d980d3ef62@SVDCMBX-MEX024.nswhealth.net> Message-ID: Where aldehydes are used for disinfection we consider 30 minutes contact time for difficult viruses like African Swine Fever (ASF) virus to be adequate.? Coronaviruses like PEDv TGE IBV and the SARS virus are much easier to inactivate than ASF virus. I'd consider the SARS virus to be similar to Infectious Bronchitis Virus which is systemic and affects a wide variety of tissues. Inactivating a virus in a chunk of tissue is more challenging than disinfection of contaminated fomites but I see no reason to think that routine fixation times would not completely inactivate the SARS coronavirus.? The concentrations of aldehyde for fixation are at least 10 times higher than the highest concentrations used when aldehydes are employed for disinfection. I'd be more concerned about the container than the contents. For necropsy samples from your human specimens there is not generally any rush to get the results so the CDC's C.Y.A. time doesn't cause trouble but waiting 72 hours for a surgical path result would seem to be just wasting time. E. Wayne Johnson DVM Enable AgTech Beijing Tony Henwood (SCHN) via Histonet wrote: > Hi Richard, > > It will depend on the size of the tissue and the source. > Lung tissue is the major concern. Other tissues not affected as much (based on the burgeoning literature on Covid-19). > Routine fixation time are applicable, remembering that the alcohols and heated wax will also inactivate the virus (triple whammy). > I suppose that I better add a reference: > > https://www.tandfonline.com/doi/full/10.1080/01478885.2020.1734718 > > > -----Original Message----- > From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, 21 April 2020 6:51 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Formalin fixation for COVID-19 positive tissues ..... > > How long are you fixing surgical tissue specimens from COVID-19 positive patient's before tissue processing? I know that the CDC is recommending "72" hours for autopsy tissues, but, to me, that seems excessive for surgical pathology specimens. Any information that you can share on this subject would be appreciated. Thank you and stay safe. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 (Office) > (860) 545-2204 (Fax) > Richard.cartun at hhchealth.org > > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang at gmx.at Tue Apr 21 03:15:06 2020 From: gu.lang at gmx.at (Gudrun Lang) Date: Tue, 21 Apr 2020 10:15:06 +0200 Subject: [Histonet] Spectra In-Reply-To: References: Message-ID: <002801d617b4$f84f3160$e8ed9420$@gmx.at> Hi! Do you use your own reagens or the Leica Spectra dyes? We have house-made ones. Too faint eosin staining can be rendered more intense with a few drops of acetic acid into the eosin to reach a pH about 5. Second, it is often a prolonged washing after the eosin that causes excess stain to be lost. Our protocol is 1 min in 2% aequoues eosin (pH 5,5), rinsing in running tapwater for 1 min, then 1 min in 96% ethanol, 2x 100%, xylen. The times for rinsing in water and 96% have to be "exact" in the protocol. In 100% and ethanol a prolonged stay doesn't matter. We optimized the turnaraound time with two identical protocols for HE, that use two staining dishes each for hematoxylin and eosin. The rest of the dishes is used by both protocols. Except of the former mentioned stations, the rest is "not exact". So the Spectra has more "ways" to find an optimal protocol. The all-over staining time is also about 60 min, due to 25 min in the oven and the progressive hematoxylin (10 min). Usually we feed the stainer continuosly in 15-20 min steps, as the racks are filled after cutting. And the racks are ready in the same pattern. Here, we have no problems with too fast lowering of the reagens-level. Hope, this helps. Gudrun -----Urspr?ngliche Nachricht----- Von: warda hassan via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Sonntag, 19. April 2020 11:37 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Spectra Good Morning to all Thanks in advance to all who would help with their feedback on the automation of H/E staining, Initially we have installed Spectra-Leica for H/E The feedback from pathologist is the stain is bit on bluish and eosin is weak We did altered and reached to 2 min of Hematoxylin and 11 min Eosin But unfortunately the stain consistency is not stable as after every 2-3 racks level of reagents reduces so we top up as we do the intensity of it differs Second issue is Turn around time, each rack takes 45mins, so tried adding up new protocol as copy but the machine keeps a gap of 12mins in picking up the slides for staining ! If anyone of you have experienced with Leica automation Please your feedback on above would be of a great help Thank you so much _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 at gmail.com Wed Apr 22 06:37:51 2020 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Wed, 22 Apr 2020 05:37:51 -0600 Subject: [Histonet] Leica's Spectra w/ coverslipper Message-ID: Good morning Histopeeps: We will be receiving our new Spectra H&E Stainer w/ cover slipper the week of May 4th. This instrument was in negotiations for purchase prior to me coming on board. I have worked with the older models of Leica?s H&E Stainers, but am unfamiliar with this one. Could any of you who has this unit be willing to share your thoughts with me. It?s been several years since I have validated a new H&E stainer. Do I need to validate the instrument along with validating and optimizing the H&E stains? Thank you in advance for your information. Akemi Allison, BS, HTL/HT (ASCP) From LRaff at uropartners.com Wed Apr 22 14:29:38 2020 From: LRaff at uropartners.com (Lester Raff MD) Date: Wed, 22 Apr 2020 19:29:38 +0000 Subject: [Histonet] Pathology perspective on COVID testing--and Happy Lab Week to all Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF12A3FEF9@COLOEXCH01.uropartners.local> http://www.chicagonow.com/downsize-maybe/2020/04/covid-testing/ Lester J. Raff, MD MBA FCAP Laboratory Medical Director UroPartners LLC Laboratory 2225 Enterprise Drive, Suite 2511 Westchester, IL 60154 Telephone: 708-486-0076 Fax: 708-492-0203 From SteveM at mcclainlab.com Thu Apr 23 05:25:40 2020 From: SteveM at mcclainlab.com (Steve McClain) Date: Thu, 23 Apr 2020 10:25:40 +0000 Subject: [Histonet] Histonet Digest, Vol 197, New Topic Coronavirus testing from tissue sample Message-ID: The CDC website with specimen submission form does not seem to be working. https://www.cdc.gov/laboratory/specimen-submission/pdf/form-50-34.pdf Does anyone know who can else can test for Corona virus from FFPE tissue? I have an unusual skin biopsy from late December (earlier than the first known US case) Thanks, Steve A. McClain, MD McClain Labs 45 Manor Road Smithtown, NY 11787 631 361-4000 cell 631 926-3655 From John.Raible at va.gov Fri Apr 24 07:10:26 2020 From: John.Raible at va.gov (Raible, John C.) Date: Fri, 24 Apr 2020 12:10:26 +0000 Subject: [Histonet] Routine H&E frozen section protocol Message-ID: Hello Histonet, My name is John Raible, and I work at the VA medical center in Minneapolis. I am trying to find routine H&E frozen section staining protocols for an automated linear stainer. My lab recently got one, and I was asked to research staining protocols Any help you could provide would be greatly appreciated. Thank you all. Stay safe and healthy! John From portera at msu.edu Fri Apr 24 07:26:28 2020 From: portera at msu.edu (Porter, Amy) Date: Fri, 24 Apr 2020 12:26:28 +0000 Subject: [Histonet] Routine H&E frozen section protocol In-Reply-To: References: Message-ID: Fix in 10% NBF - 10 minutes Rinse in running tap water - 5 minute Rinse in distilled water Gill 2 Hematoxylin - 1 1/2 minutes 1% Glacial Acetic water - 3 dips Running water - 2 minutes (we do not need bluing agent - our water takes care of it) 95% Ethanol -2 minutes 1% Eosin/Phloxine - 2 minutes 95% Ethanol - 2 minutes Remaining dehydration in absolute ethanol & xylene - 2 mintues rest of the way down ________________________________ From: Raible, John C. via Histonet Sent: Friday, April 24, 2020 8:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Routine H&E frozen section protocol Hello Histonet, My name is John Raible, and I work at the VA medical center in Minneapolis. I am trying to find routine H&E frozen section staining protocols for an automated linear stainer. My lab recently got one, and I was asked to research staining protocols Any help you could provide would be greatly appreciated. Thank you all. Stay safe and healthy! John _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!jZVhszUj4q_tZtfO2cKIo_OQbyOsjHXT6pEGAn_UXytcWKRzM703NRorymJh6A$ From criley at dpspa.com Fri Apr 24 11:13:58 2020 From: criley at dpspa.com (Charles Riley) Date: Fri, 24 Apr 2020 12:13:58 -0400 Subject: [Histonet] FFPE PRC testing Message-ID: What platform does everyone use to carry out FFPE PCR testing? Seeking to bring in as many genetic mutation testing options as possible (BRAF, KRAS, NRAS...etc.) -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From Kelly.Pairan at nationwidechildrens.org Fri Apr 24 12:02:17 2020 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Fri, 24 Apr 2020 17:02:17 +0000 Subject: [Histonet] Embedding Station Recommendations Message-ID: <56bc37e4549c45a89081b282d67c47ba@l1perdwmbx01.childrensroot.net> Good Afternoon and Happy Lab Week, We are currently looking into replacing a Leica 1150H embedding station. We love the size of that instrument's hot plate and unfortunately the newer version (Arcadia) has a much narrower one. We have also reached out to Sakura but their latest embedding center also has a pretty narrow hotplate. Does anyone have a recommendation of another company other than Sakura and Leica? Thanks, Kelly Kelly Pairan, BS, HT (ASCP)CM, QIHC (ASCP) Histology Supervisor-Anatomic Pathology Department of Pathology and Laboratory Medicine Email: kelly.pairan at nationwidechildrens.org ph: 614-722-5414 fx: 614-722-3033 From tbraud at holyredeemer.com Fri Apr 24 12:53:59 2020 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 24 Apr 2020 17:53:59 +0000 Subject: [Histonet] linear stainer protocol Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C4BD7833@HRHEX02-HOS.holyredeemer.local> We have the Leica linear stainer (Also sold by Avantik?) and have it set at 7 second intervals. The stain takes about 100 seconds. Slides are fixed in the Coplin jar with 95% Alcohol then loaded: The stations are in order: 1. Water wash 2. Hemotoxylin (Richard Allen 7211) 3. Hemotoxylin (Richard Allen 7211) 4. Hemotoxylin (Richard Allen 7211) 5. Water wash 6. Bluing (we use Surgipath SelecTech Blue Buffer 8 concentrate, diluted according to directions 7. Water wash 8. Eosin (1% Alcoholic) 9. 95% Alcohol 10. Absolute Alcohol 11. Absolute Alcohol 12. Absolute Alcohol 13. Xylene 14. Xylene The slides are clean and consistent with great differentiation. The Richard Allen 7211 Hematoxylin does not require filtering and seems to last forever. I sincerely hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Routine H&E frozen section protocol (Raible, John C.) 2. Re: Routine H&E frozen section protocol (Porter, Amy) 3. FFPE PRC testing (Charles Riley) ---------------------------------------------------------------------- Message: 1 Date: Fri, 24 Apr 2020 12:10:26 +0000 From: "Raible, John C." To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Routine H&E frozen section protocol Message-ID: Content-Type: text/plain; charset="us-ascii" Hello Histonet, My name is John Raible, and I work at the VA medical center in Minneapolis. I am trying to find routine H&E frozen section staining protocols for an automated linear stainer. My lab recently got one, and I was asked to research staining protocols Any help you could provide would be greatly appreciated. Thank you all. Stay safe and healthy! John ------------------------------ Message: 2 Date: Fri, 24 Apr 2020 12:26:28 +0000 From: "Porter, Amy" To: "histonet at lists.utsouthwestern.edu" , "Raible, John C." Subject: Re: [Histonet] Routine H&E frozen section protocol Message-ID: Content-Type: text/plain; charset="iso-8859-1" Fix in 10% NBF - 10 minutes Rinse in running tap water - 5 minute Rinse in distilled water Gill 2 Hematoxylin - 1 1/2 minutes 1% Glacial Acetic water - 3 dips Running water - 2 minutes (we do not need bluing agent - our water takes care of it) 95% Ethanol -2 minutes 1% Eosin/Phloxine - 2 minutes 95% Ethanol - 2 minutes Remaining dehydration in absolute ethanol & xylene - 2 mintues rest of the way down ________________________________ From: Raible, John C. via Histonet Sent: Friday, April 24, 2020 8:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Routine H&E frozen section protocol Hello Histonet, My name is John Raible, and I work at the VA medical center in Minneapolis. I am trying to find routine H&E frozen section staining protocols for an automated linear stainer. My lab recently got one, and I was asked to research staining protocols Any help you could provide would be greatly appreciated. Thank you all. Stay safe and healthy! John _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!jZVhszUj4q_tZtfO2cKIo_OQbyOsjHXT6pEGAn_UXytcWKRzM703NRorymJh6A$ ------------------------------ Message: 3 Date: Fri, 24 Apr 2020 12:13:58 -0400 From: Charles Riley To: Histo List Subject: [Histonet] FFPE PRC testing Message-ID: Content-Type: text/plain; charset="UTF-8" What platform does everyone use to carry out FFPE PCR testing? Seeking to bring in as many genetic mutation testing options as possible (BRAF, KRAS, NRAS...etc.) -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 197, Issue 22 ***************************************** From SteveM at mcclainlab.com Sat Apr 25 12:51:54 2020 From: SteveM at mcclainlab.com (Steve McClain) Date: Sat, 25 Apr 2020 17:51:54 +0000 Subject: [Histonet] Histonet Digest, Vol 197, Issue 23 recovering RNA from FFPE Message-ID: 1) Does anyone know of a method of recovering RNA from FFPE? 2) Does anyone know a lab that has done it? Thanks, Steve A. McClain, MD McClain Labs 45 Manor Road Smithtown, NY 11787 631 361-4000 cell 631 926-3655 From paula at excaliburpathology.com Sat Apr 25 13:51:53 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Sat, 25 Apr 2020 18:51:53 +0000 (UTC) Subject: [Histonet] Histonet Digest, Vol 197, Issue 23 recovering RNA from FFPE In-Reply-To: References: Message-ID: <2098100414.165000.1587840713233@mail.yahoo.com> By doing an internet search for FFPE PCR Platform, Thermo, Agilent, Biogen, Qiagen, etc. all have this. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Saturday, April 25, 2020, 01:08:53 PM CDT, Steve McClain via Histonet wrote: 1) Does anyone know of a method of recovering RNA from FFPE? 2) Does anyone know a lab that has done it? Thanks, Steve A. McClain, MD McClain Labs 45 Manor Road Smithtown, NY 11787 631 361-4000 cell 631 926-3655 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paula at excaliburpathology.com Sat Apr 25 13:53:12 2020 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Sat, 25 Apr 2020 18:53:12 +0000 (UTC) Subject: [Histonet] Histonet Digest, Vol 197, Issue 23 recovering RNA from FFPE In-Reply-To: References: Message-ID: <1255227197.161532.1587840792222@mail.yahoo.com> Biochain too. Paula On Saturday, April 25, 2020, 01:08:53 PM CDT, Steve McClain via Histonet wrote: 1) Does anyone know of a method of recovering RNA from FFPE? 2) Does anyone know a lab that has done it? Thanks, Steve A. McClain, MD McClain Labs 45 Manor Road Smithtown, NY 11787 631 361-4000 cell 631 926-3655 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford at DignityHealth.org Mon Apr 27 13:35:06 2020 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Mon, 27 Apr 2020 18:35:06 +0000 Subject: [Histonet] Printmate/Slidemate Message-ID: <2bf30f03d7c0401780ff4b73137ba62e@PHX-EXCH-013.chw.edu> Good Morning, I was wondering if anyone has a procedure written up on how to train someone for the Thermo Printmate AS and Slidemate AS that I could get? This would be for my Policy and Procedure manual. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. From emmanuelg at gmail.com Wed Apr 29 11:36:35 2020 From: emmanuelg at gmail.com (Emmanuel Gonzaga) Date: Wed, 29 Apr 2020 09:36:35 -0700 Subject: [Histonet] Covid-19 ihc testing Message-ID: Hello all, Does anyone know of a lab doing covid-19 ihc testing? Thanks. From deedeebrina at aol.com Thu Apr 30 12:49:13 2020 From: deedeebrina at aol.com (Dionee Neamand) Date: Thu, 30 Apr 2020 17:49:13 +0000 (UTC) Subject: [Histonet] embedding and cutting per tech References: <1936702841.379717.1588268953914.ref@mail.yahoo.com> Message-ID: <1936702841.379717.1588268953914@mail.yahoo.com> Hi. Does anyone have a reference to how many blocks and slides per tech per day are appropriate? I'm trying to figure out how quickly to bring back techs from furlough as we slowly open up elective procedures again at our institution. Any information would be greatly appreciated! Thanks,Dee Dionee Neamand ? ?Leach TechnicianHistology Technician II ? ?Evangelical Community HospitalLewisburg? PAevanhospital.com