From SGower at pathgroup.com Tue Sep 3 11:54:33 2019 From: SGower at pathgroup.com (Shannon E. Gower) Date: Tue, 3 Sep 2019 16:54:33 +0000 Subject: [Histonet] Histology position in Virginia Message-ID: Hello Histo colleagues! We currently have an opening for a histologist in beautiful Roanoke, VA. M-F 5a-130p. I'm looking for an experienced embedder and microtomist who is ready for a fast paced environment. We boast a quick TAT providing quality pathology services for our local hospital system. Shift includes some special stains and IHCs (all automated) as well as an opportunity for cytology if desired. Please email me directly if you are interested. Have a great day! Shannon Gower, HT(ASCP) QIHC Histology Manager PathGroup Lewis Gale Medical Center 1802 Braeburn Drive Salem, Virginia 24153 phone: 540-588-8735 fax: 540-777-1449 sgower at pathgroup.com www.pathgroup.com Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Chief Privacy Officer at PathGroup immediately at 615-695-4987. Thank you From CDavis at che-east.org Tue Sep 3 12:32:27 2019 From: CDavis at che-east.org (Cassie P. Davis) Date: Tue, 3 Sep 2019 17:32:27 +0000 Subject: [Histonet] Reprocessing tissue Message-ID: Has anyone had success with just melting down unfaced, underprocessed blocks for reprocessing and sticking them in NBF for processing without taking them through the clean cycle? The senior tech is doing this and I am concerned about our processor getting paraffin in the NBF and other things. Cassie Davis Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From aperl at caremount.com Tue Sep 3 12:42:25 2019 From: aperl at caremount.com (Perl , Alison) Date: Tue, 3 Sep 2019 17:42:25 +0000 Subject: [Histonet] Reprocessing tissue In-Reply-To: References: Message-ID: <6a5f04fc6c8e4c1799266a7e717b139b@MK-EXMB02.mkmg.com> Hi Cassie How often is this happening? We have done it this way, with no ill effects on the processor, but it comes up maybe once every few months - if you have to reprocess tissue daily or weekly, I could see it adding up to a problem. We melt the block, blot the tissue and cassette with gauze to remove as much paraffin as possible, then put in the rack with new work, and usually comes out fine. Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at caremount.com -----Original Message----- From: Cassie P. Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 03, 2019 1:32 PM To: histonet Subject: [EXTERNAL] [Histonet] Reprocessing tissue Has anyone had success with just melting down unfaced, underprocessed blocks for reprocessing and sticking them in NBF for processing without taking them through the clean cycle? The senior tech is doing this and I am concerned about our processor getting paraffin in the NBF and other things. Cassie Davis Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDavis at che-east.org Tue Sep 3 12:54:13 2019 From: CDavis at che-east.org (Cassie P. Davis) Date: Tue, 3 Sep 2019 17:54:13 +0000 Subject: [Histonet] Reprocessing tissue In-Reply-To: <6a5f04fc6c8e4c1799266a7e717b139b@MK-EXMB02.mkmg.com> References: , <6a5f04fc6c8e4c1799266a7e717b139b@MK-EXMB02.mkmg.com> Message-ID: Thank to those who responded so quickly. It happens maybe once a quarter. It happened today because the programs got switched: large tissue processed on biopsy program. I've heard of folks doing it this way with the theroy the paraffin protects the processed tissue. I'm just old school I guess. I don't know if this has been vaildated, I will have to ask our Lead when she gets back from vacation. Thanks for teaching me my something new today! Cassie Davis Histology Department Anatomical Pathology Laboratory 302-575-8095 Email: CDavis at che-east.org ________________________________ From: Perl , Alison Sent: Tuesday, September 3, 2019 12:42 PM To: Cassie P. Davis Cc: 'Cassie P. Davis via Histonet' Subject: [External] RE: Reprocessing tissue Warning: This email originated from the Internet! DO NOT CLICK links if the sender is unknown, and NEVER provide your password. Hi Cassie How often is this happening? We have done it this way, with no ill effects on the processor, but it comes up maybe once every few months - if you have to reprocess tissue daily or weekly, I could see it adding up to a problem. We melt the block, blot the tissue and cassette with gauze to remove as much paraffin as possible, then put in the rack with new work, and usually comes out fine. Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at caremount.com -----Original Message----- From: Cassie P. Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 03, 2019 1:32 PM To: histonet Subject: [EXTERNAL] [Histonet] Reprocessing tissue Has anyone had success with just melting down unfaced, underprocessed blocks for reprocessing and sticking them in NBF for processing without taking them through the clean cycle? The senior tech is doing this and I am concerned about our processor getting paraffin in the NBF and other things. Cassie Davis Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From simmca at UPMC.EDU Tue Sep 3 16:20:50 2019 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Tue, 3 Sep 2019 21:20:50 +0000 Subject: [Histonet] Reprocessing tissue In-Reply-To: References: , <6a5f04fc6c8e4c1799266a7e717b139b@MK-EXMB02.mkmg.com>, Message-ID: There are several papers on this. We did a purposeful underprocess and cut and stained it, then reprocessed as normal and compared. The results were eye-opening in terms of how good it worked, but you need to factor in that the second processing will diminish IHC staining quality. Get Outlook for iOS ________________________________ From: Cassie P. Davis via Histonet Sent: Tuesday, September 3, 2019 1:54:13 PM To: Perl , Alison Cc: 'Cassie P. Davis via Histonet' Subject: Re: [Histonet] Reprocessing tissue Thank to those who responded so quickly. It happens maybe once a quarter. It happened today because the programs got switched: large tissue processed on biopsy program. I've heard of folks doing it this way with the theroy the paraffin protects the processed tissue. I'm just old school I guess. I don't know if this has been vaildated, I will have to ask our Lead when she gets back from vacation. Thanks for teaching me my something new today! Cassie Davis Histology Department Anatomical Pathology Laboratory 302-575-8095 Email: CDavis at che-east.org ________________________________ From: Perl , Alison Sent: Tuesday, September 3, 2019 12:42 PM To: Cassie P. Davis Cc: 'Cassie P. Davis via Histonet' Subject: [External] RE: Reprocessing tissue Warning: This email originated from the Internet! DO NOT CLICK links if the sender is unknown, and NEVER provide your password. Hi Cassie How often is this happening? We have done it this way, with no ill effects on the processor, but it comes up maybe once every few months - if you have to reprocess tissue daily or weekly, I could see it adding up to a problem. We melt the block, blot the tissue and cassette with gauze to remove as much paraffin as possible, then put in the rack with new work, and usually comes out fine. Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at caremount.com -----Original Message----- From: Cassie P. Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 03, 2019 1:32 PM To: histonet Subject: [EXTERNAL] [Histonet] Reprocessing tissue Has anyone had success with just melting down unfaced, underprocessed blocks for reprocessing and sticking them in NBF for processing without taking them through the clean cycle? The senior tech is doing this and I am concerned about our processor getting paraffin in the NBF and other things. Cassie Davis Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKnutson at primecare.org Wed Sep 4 10:39:00 2019 From: DKnutson at primecare.org (Knutson, Deanne) Date: Wed, 4 Sep 2019 10:39:00 -0500 Subject: [Histonet] CPT Code 88172 Message-ID: <1E0E2B14C709174B8AC2BE0AE7F768330159B57565A4@EXCHANGE2K7.staprimecare.org> Hello Histonetters! I am wondering how others may be using the cpt code 88172-T? Like in what type of scenarios? Thank you for your responses. Deanne Knutson Supervisor Anatomic Pathology dknutson at primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From jwwalker at rrmc.org Wed Sep 4 10:53:28 2019 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Wed, 4 Sep 2019 15:53:28 +0000 Subject: [Histonet] CPT Code 88172 In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F768330159B57565A4@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F768330159B57565A4@EXCHANGE2K7.staprimecare.org> Message-ID: Hi Deanne, We utilize the 88172-TC to capture the work that our cytotechs put into assisting with the FNA procedures. This covers them making slides, staining them and triaging the specimen for any ancillary testing that might be needed. The CPT code manual states, " Cytopathology, evaluation of fine needle aspirate; immediate cytohistologic study to determine adequacy for diagnosis, first evaluation episode, each site". This code is used in conjunction with the 88177, which is for additional passes at the same site. Keep in mind that these codes are also accompanied by 88173, which is defined as " Cytopathology, evaluation of fine needle aspirate; interpretation and report". If a pathologist was not present to provide the immediate read for adequacy, then you may run into difficulties charging the TC for the 88172 and 88177 depending on the payer. Does this help? Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Knutson, Deanne via Histonet Sent: Wednesday, September 4, 2019 11:39 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] CPT Code 88172 [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. Hello Histonetters! I am wondering how others may be using the cpt code 88172-T? Like in what type of scenarios? Thank you for your responses. Deanne Knutson Supervisor Anatomic Pathology dknutson at primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7C6ea0323068a24ee67c3c08d7314e409a%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C637032084468590907&sdata=DqnTmRQ3EiAQpfGNhBHFrKljuNO5GjKNlEQVZAbw%2FEI%3D&reserved=0 [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] From twebster at CRH.org Wed Sep 4 12:31:03 2019 From: twebster at CRH.org (Webster, Thomas S.) Date: Wed, 4 Sep 2019 17:31:03 +0000 Subject: [Histonet] CPT Code 88172 Message-ID: You can only bill for 88172 if the pathologist looks at the FNA slide(s) during the procedure. Otherwise do not attempt to bill for it. It is a physician code. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From jwwalker at rrmc.org Wed Sep 4 13:07:26 2019 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Wed, 4 Sep 2019 18:07:26 +0000 Subject: [Histonet] CPT Code 88172 In-Reply-To: References: Message-ID: Hi Thomas, What is your reference source for stating this is "a physician code"? In reviewing our coding data, this code carries a facilities component in addition to a professional component. I agree that a pathologist must perform the review in order to bill for both, but I am aware of people in New England who bill just the TC when a technologist provides the adequacy interpretation and some payers cover this charge. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Webster, Thomas S. via Histonet Sent: Wednesday, September 4, 2019 1:31 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] CPT Code 88172 [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. You can only bill for 88172 if the pathologist looks at the FNA slide(s) during the procedure. Otherwise do not attempt to bill for it. It is a physician code. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7Cc6674fb9f46141aa02c008d7315dc983%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C637032151201028134&sdata=AbLhd3nb7zc%2Bh2ytKU9qGqVg4wxffGvzrcC5wrXJ6cE%3D&reserved=0 [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] From bakevictoria at gmail.com Wed Sep 4 13:44:20 2019 From: bakevictoria at gmail.com (Victoria Baker) Date: Wed, 4 Sep 2019 14:44:20 -0400 Subject: [Histonet] CPT Code 88172 In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F768330159B57565A4@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F768330159B57565A4@EXCHANGE2K7.staprimecare.org> Message-ID: Hi, I just referred back to my sometimes frustrating clear as mud 2019 CPT coding binder. 88172 is not saying that it has to be done by a pathologist. In many cases it is completed by a Cytotechnologist. The precise wording says "Cytopathology, evaluation of fine needle aspirate; immediate cytohistologic study to determine adequacy for diagnosis, first evaluation episode each site The professional code comes in on 88173 where it involves the interpretation and report. The stance that it is a tech component comes from the Cytologist/pathologist obtaining the specimen. However they are responsible for the adequacy of of the specimen. The argument goes both ways. At this institution we use it as a professional code only. Vikki On Wed, Sep 4, 2019, 11:43 AM Knutson, Deanne via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello Histonetters! > > I am wondering how others may be using the cpt code 88172-T? Like in what > type of scenarios? > > Thank you for your responses. > > Deanne Knutson > Supervisor > Anatomic Pathology > dknutson at primecare.org > > > > > > > ________________________________ > This email may include confidential and privileged information. If this is > not intended for your use, please destroy immediately and contact the > sender of the message. > > This email and attachments contain information that may be confidential or > privileged. If you are not the intended recipient, notify the sender at > once and delete this message completely from your information system. > Further use, disclosure, or copying of information contained in this email > is not authorized, and any such action should not be construed as a waiver > of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From BAMoe at gundersenhealth.org Wed Sep 4 14:47:30 2019 From: BAMoe at gundersenhealth.org (Moe, Barbi A) Date: Wed, 4 Sep 2019 19:47:30 +0000 Subject: [Histonet] PPE for frozen sections Message-ID: Could anyone share what PPE you are required to wear when performing a frozen section? If doing a frozen section on a piece of lung or mediastinal nodes are you required to wear a respirator such as N95 or even a PAP-R? Any thoughts are appreciated. Barb Moe Histology Lab Gundersen Health Systems La Crosse WI bamoe at gundersenhealth.org From BAMoe at gundersenhealth.org Wed Sep 4 14:51:41 2019 From: BAMoe at gundersenhealth.org (Moe, Barbi A) Date: Wed, 4 Sep 2019 19:51:41 +0000 Subject: [Histonet] DAKO Artisan special stain kit validations Message-ID: For those that are using the DAKO Artisan for special stains could you comment on how you are handling implementing new kits? When a new lot number on a kit is received do you run a positive AND negative control either prior to use or concurrent with a patient case? Any thoughts are appreciated. Barb Moe Histology Lab Gundersen Health System La Crosse WI bamoe at gundersenhealth.org From BAMoe at gundersenhealth.org Wed Sep 4 15:25:59 2019 From: BAMoe at gundersenhealth.org (Moe, Barbi A) Date: Wed, 4 Sep 2019 20:25:59 +0000 Subject: [Histonet] Cryostat to room temperature Message-ID: Does anyone know how long it would take a cryostat that is maintained at approx. -22 degrees C to come to room temperature after being turned off? Any thoughts are appreciated. Barb Moe Histology Lab Gundersen Health Systems La Crosse WI bamoe at gundersenhealth.org From PREISZNE at mail.etsu.edu Thu Sep 5 12:55:43 2019 From: PREISZNE at mail.etsu.edu (Preiszner, Johanna) Date: Thu, 5 Sep 2019 17:55:43 +0000 Subject: [Histonet] How do you section PPFE tissue in embedding rings? Message-ID: Hi, I received a lot of specimens embedded in plastic embedding rings...have a Leica RM2135 microtome. How can I put these blocks in the clamps of the microtome? Searched Internet with zero success. Nobody in town ever used embedding rings so I can not borrrow an adapter if that's what I need. Could anybody please help out? Re-embedding the tissue in regular cassettes would cost the client a lot of money. Thanks, Johanna Preiszner From whitmorel at mindspring.com Thu Sep 5 13:52:47 2019 From: whitmorel at mindspring.com (Lynn Whitmore) Date: Thu, 5 Sep 2019 14:52:47 -0400 (GMT-04:00) Subject: [Histonet] Cryostat cooling time Message-ID: <559455079.7691.1567709567688@wamui-agami.atl.sa.earthlink.net> The amount of time a cryostat takes to get to cutting temperature depends on the age of the machine, manufacturer, and air flow around the cryostat. We have 4 Avantiks the newer ones cool faster than the older ones and the one that is under a wall cabinet with about 2 feet from the top of the cryostat to the bottom of the cabinet takes about an hour longer to cool. The cryostats are kept at -27. The cooling time for the quicker one is 2 hours from room temperature (70 degrees Fahrenheit) and the slower one is 3 hours. I would suggest you plan for 4 hours and monitor the cooling time so you know for the next time you need to shutdown the cryostat. I have had Leicas and Thermo Fisher cryostats and they were different. Hope this helps. -----Original Message----- >From: histonet-request at lists.utsouthwestern.edu >Sent: Sep 5, 2019 1:00 PM >To: histonet at lists.utsouthwestern.edu >Subject: Histonet Digest, Vol 190, Issue 3 > >Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." > > >Today's Topics: > > 1. Re: CPT Code 88172 (Webster, Thomas S.) > 2. Re: CPT Code 88172 (Joe W. Walker, Jr.) > 3. Re: CPT Code 88172 (Victoria Baker) > 4. PPE for frozen sections (Moe, Barbi A) > 5. DAKO Artisan special stain kit validations (Moe, Barbi A) > 6. Cryostat to room temperature (Moe, Barbi A) > > >---------------------------------------------------------------------- > >Message: 1 >Date: Wed, 4 Sep 2019 17:31:03 +0000 >From: "Webster, Thomas S." >To: "'histonet at lists.utsouthwestern.edu'" > >Subject: Re: [Histonet] CPT Code 88172 >Message-ID: >Content-Type: text/plain; charset="us-ascii" > >You can only bill for 88172 if the pathologist looks at the FNA slide(s) during the procedure. Otherwise do not attempt to bill for it. It is a physician code. > > >CONFIDENTIALITY NOTICE: >This e-mail message, including all attachments, is for the sole use of the >intended recipient(s) and may contain confidential and privileged >information. You may NOT use, disclose, copy or disseminate this >information. If you are not the intended recipient, please contact the >sender by reply e-mail immediately. Please destroy all copies of the >original message and all attachments. Your cooperation is greatly >appreciated. >Columbus Regional Hospital >2400 East 17th Street >Columbus, Indiana 47201 > >------------------------------ > >Message: 2 >Date: Wed, 4 Sep 2019 18:07:26 +0000 >From: "Joe W. Walker, Jr." >To: "Webster, Thomas S." >Cc: "histonet at lists.utsouthwestern.edu" > >Subject: Re: [Histonet] CPT Code 88172 >Message-ID: > > >Content-Type: text/plain; charset="utf-8" > >Hi Thomas, > >What is your reference source for stating this is "a physician code"? In reviewing our coding data, this code carries a facilities component in addition to a professional component. I agree that a pathologist must perform the review in order to bill for both, but I am aware of people in New England who bill just the TC when a technologist provides the adequacy interpretation and some payers cover this charge. > >Joe W. Walker, Jr. MS, SCT(ASCP) >Anatomical Pathology Manager >joewalker at rrmc.org, www.rrmc.org > >-----Original Message----- >From: Webster, Thomas S. via Histonet >Sent: Wednesday, September 4, 2019 1:31 PM >To: 'histonet at lists.utsouthwestern.edu' >Subject: Re: [Histonet] CPT Code 88172 > >[External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. > > >You can only bill for 88172 if the pathologist looks at the FNA slide(s) during the procedure. Otherwise do not attempt to bill for it. It is a physician code. > > >CONFIDENTIALITY NOTICE: >This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. >Columbus Regional Hospital >2400 East 17th Street >Columbus, Indiana 47201 >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7Cc6674fb9f46141aa02c008d7315dc983%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C637032151201028134&sdata=AbLhd3nb7zc%2Bh2ytKU9qGqVg4wxffGvzrcC5wrXJ6cE%3D&reserved=0 >[https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] > >------------------------------ > >Message: 3 >Date: Wed, 4 Sep 2019 14:44:20 -0400 >From: Victoria Baker >To: "Knutson, Deanne" >Cc: Histo Net list server >Subject: Re: [Histonet] CPT Code 88172 >Message-ID: > >Content-Type: text/plain; charset="UTF-8" > >Hi, > >I just referred back to my sometimes frustrating clear as mud 2019 CPT >coding binder. >88172 is not saying that it has to be done by a pathologist. In many cases >it is completed by a Cytotechnologist. >The precise wording says >"Cytopathology, evaluation of fine needle aspirate; immediate >cytohistologic study to determine adequacy for diagnosis, first evaluation >episode each site >The professional code comes in on 88173 where it involves the >interpretation and report. > >The stance that it is a tech component comes from the >Cytologist/pathologist obtaining the specimen. However they are >responsible for the adequacy of of the specimen. The argument goes both >ways. > >At this institution we use it as a professional code only. > >Vikki > >On Wed, Sep 4, 2019, 11:43 AM Knutson, Deanne via Histonet < >histonet at lists.utsouthwestern.edu> wrote: > >> Hello Histonetters! >> >> I am wondering how others may be using the cpt code 88172-T? Like in what >> type of scenarios? >> >> Thank you for your responses. >> >> Deanne Knutson >> Supervisor >> Anatomic Pathology >> dknutson at primecare.org >> >> >> >> >> >> >> ________________________________ >> This email may include confidential and privileged information. If this is >> not intended for your use, please destroy immediately and contact the >> sender of the message. >> >> This email and attachments contain information that may be confidential or >> privileged. If you are not the intended recipient, notify the sender at >> once and delete this message completely from your information system. >> Further use, disclosure, or copying of information contained in this email >> is not authorized, and any such action should not be construed as a waiver >> of privilege or other confidentiality protections. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > >------------------------------ > >Message: 4 >Date: Wed, 4 Sep 2019 19:47:30 +0000 >From: "Moe, Barbi A" >To: "histonet at lists.utsouthwestern.edu" > >Subject: [Histonet] PPE for frozen sections >Message-ID: > > >Content-Type: text/plain; charset="us-ascii" > >Could anyone share what PPE you are required to wear when performing a frozen section? > >If doing a frozen section on a piece of lung or mediastinal nodes are you required to wear a respirator such as N95 or even a PAP-R? > >Any thoughts are appreciated. > >Barb Moe >Histology Lab >Gundersen Health Systems >La Crosse WI >bamoe at gundersenhealth.org > > >------------------------------ > >Message: 5 >Date: Wed, 4 Sep 2019 19:51:41 +0000 >From: "Moe, Barbi A" >To: "histonet at lists.utsouthwestern.edu" > >Subject: [Histonet] DAKO Artisan special stain kit validations >Message-ID: > > >Content-Type: text/plain; charset="us-ascii" > >For those that are using the DAKO Artisan for special stains could you comment on how you are handling implementing new kits? > >When a new lot number on a kit is received do you run a positive AND negative control either prior to use or concurrent with a patient case? > >Any thoughts are appreciated. > >Barb Moe >Histology Lab >Gundersen Health System >La Crosse WI >bamoe at gundersenhealth.org > > >------------------------------ > >Message: 6 >Date: Wed, 4 Sep 2019 20:25:59 +0000 >From: "Moe, Barbi A" >To: "histonet at lists.utsouthwestern.edu" > >Subject: [Histonet] Cryostat to room temperature >Message-ID: > > >Content-Type: text/plain; charset="us-ascii" > >Does anyone know how long it would take a cryostat that is maintained at approx. -22 degrees C to come to room temperature after being turned off? > >Any thoughts are appreciated. > >Barb Moe >Histology Lab >Gundersen Health Systems >La Crosse WI >bamoe at gundersenhealth.org > > > >------------------------------ > >Subject: Digest Footer > >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >------------------------------ > >End of Histonet Digest, Vol 190, Issue 3 >**************************************** From paula at excaliburpathology.com Thu Sep 5 14:04:14 2019 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Thu, 5 Sep 2019 19:04:14 +0000 (UTC) Subject: [Histonet] How do you section PPFE tissue in embedding rings? In-Reply-To: References: Message-ID: <1913092966.1900600.1567710254815@mail.yahoo.com> Hi, I used embedding rings back in the 80s and still use the chuck adapter for large blocks. I do eyes. I have a couple John's Hopkins chuck adapters and could loan you one. Send me a picture of your microtome with a closeup of the chuck adapter to see if it will fit. Do you have an old AO 820 sitting around? I know it will fit it. The old Leica 1512s had an adapter that would work too. Otherwise, I suggest just re-embedding. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, September 5, 2019, 01:16:21 PM CDT, Preiszner, Johanna via Histonet wrote: Hi, I received a lot of specimens embedded in plastic embedding rings...have a Leica RM2135 microtome. How can I put these blocks in the clamps of the microtome? Searched Internet with zero success. Nobody in town ever used embedding rings so I can not borrrow an adapter if that's what I need. Could anybody please help out? Re-embedding the tissue in regular cassettes would cost the client a lot of money. Thanks, Johanna Preiszner _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From swchang at unitedmedicallab.com Thu Sep 5 15:25:51 2019 From: swchang at unitedmedicallab.com (Dr. Stephen Chang [UML]) Date: Thu, 5 Sep 2019 16:25:51 -0400 Subject: [Histonet] Leica Bond Max - non OEM products? Message-ID: <044301d56428$1ce7cb40$56b761c0$@unitedmedicallab.com> Hello, I was referred to join this listserv due to a question we have about our Leica Bond Max. I work in a medical diagnostic laboratory. Does anyone know where we can get good quality but cheaper reagents to use on the Leica Bond Max, perhaps from a third party? The prices from Leica directly are somewhat pricey and the person who referred me said she believes that many labs use "aftermarket" supplies. I'm talking about epitope retrieval and wash solution, etc, not antibodies. Thanks in advance! Sincerely, Stephen W. Chang, MD, MBA United Medical Laboratories 1980 Gallows Rd, Ste 300 Tysons, VA 22182 703-356-4422 (phone) 703-356-2460 (fax) www.UnitedMedicalLab.com This message contains confidential information and is intended only for the individual(s) named. If you are not the named addressee you should not disseminate, distribute, or copy this email. You cannot use or forward any attachments in the email. If you have received this message in error, please notify the sender immediately and then delete this email (including any attachments) from your system. From Prabhakar.Ashwin at hcahealthcare.com Thu Sep 5 16:55:39 2019 From: Prabhakar.Ashwin at hcahealthcare.com (Ashwin Ashwin) Date: Thu, 5 Sep 2019 21:55:39 +0000 Subject: [Histonet] Tissue Processor Validation Message-ID: <13CFD2CE-F5E7-4915-BD1B-E4663E2E2DF9@hcahealthcare.com> Hello Everyone, If we change a processing protocol and are in need to recalibrate our tissue processors, is it required to perform concordance study between the old protocol and the new? Please let me know Thanks Sent from my iPhone From Prabhakar.Ashwin at hcahealthcare.com Thu Sep 5 16:59:26 2019 From: Prabhakar.Ashwin at hcahealthcare.com (Ashwin Ashwin) Date: Thu, 5 Sep 2019 21:59:26 +0000 Subject: [Histonet] Florida Cytology Message-ID: <1CB30DE2-75A9-41E0-99A5-6B8ECDB06057@hcahealthcare.com> Hello, Do we need to have a licensed Histotech to perform pre-analytical non-gyn Cytology slide prep? Kindly advise Thanks Sent from my iPhone From angela at lji.org Fri Sep 6 08:46:22 2019 From: angela at lji.org (Angela Denn) Date: Fri, 6 Sep 2019 06:46:22 -0700 Subject: [Histonet] looking to buy a Tissue Tek VIP 5 Message-ID: Hello, I am actively seeking quotes for a refurbished VIP 5. Please include the cost of the unit with shipping/delivery (92037) as well as any warranty information. I do have a VIP E150 to trade. If the unit is not in stock, a projected time for procurement would be useful as I'm looking to puchase asap. -- *Angela Lamberth Denn* | Histology Technician III angela at lji.org *La Jolla Institute for Immunology* Histology Core, Room 2328 9420 Athena Circle La Jolla, CA 92037 http://www.lji.org From angela at lji.org Fri Sep 6 10:28:37 2019 From: angela at lji.org (Angela Denn) Date: Fri, 6 Sep 2019 08:28:37 -0700 Subject: [Histonet] looking to buy a Tissue Tek VIP 5 In-Reply-To: References: Message-ID: I've uploaded some pics of my VIP E150 that I'm looking to trade in. https://imgur.com/a/mLpEpY8 On Fri, Sep 6, 2019 at 6:46 AM Angela Denn wrote: > Hello, I am actively seeking quotes for a refurbished VIP 5. Please > include the cost of the unit with shipping/delivery (92037) as well as any > warranty information. I do have a VIP E150 to trade. If the unit is not in > stock, a projected time for procurement would be useful as I'm looking to > puchase asap. > > -- > *Angela Lamberth Denn* | Histology Technician III > angela at lji.org > > *La Jolla Institute for Immunology* > Histology Core, Room 2328 > 9420 Athena Circle La Jolla, CA 92037 > http://www.lji.org > > > > > > -- *Please email Histology at lji.org for work order requests or inquiries.* *Angela Lamberth Denn* | Histology Technician III angela at lji.org *La Jolla Institute for Immunology* Histology Core, Room 2328 9420 Athena Circle La Jolla, CA 92037 http://www.lji.org 858.752.6500 From erin.mccarthy at tempus.com Fri Sep 6 17:05:39 2019 From: erin.mccarthy at tempus.com (Erin McCarthy) Date: Fri, 6 Sep 2019 17:05:39 -0500 Subject: [Histonet] Tissue scrolling Message-ID: Hello Histonetters, I am curious if any of you perform scrolling of paraffin embedded tissue for molecular studies? If so, what precautions do you take to minimize contamination? I am asking specifically about at the cutting station as that is my area of expertise. But we are looking to pilot this for samples that in the past have had large quantities of recuts. Thank you for any information you can provide! -- Erin McCarthy, HT (ASCP) Histology Supervisor Tempus Labs 600 W. Chicago Ave. Chicago IL 60654 Cell: (708)269-8610 -- This email and any attachments may contain privileged and confidential information and/or protected health information (PHI) that is protected by federal and state privacy laws.? It is intended solely for the use of Tempus Labs and the recipient(s) named above.? Nothing contained in this communication and any attachments thereto is intended to waive any privileges or rights of confidentiality.? If you are not the recipient, or the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any review, dissemination, distribution, printing or copying of this email message and/or any attachments is strictly prohibited.?* If you have received this transmission in error, please notify us immediately at?**(855)-442-8305**? and permanently delete this email and any attachments*. From john.garratt at ciqc.ca Sun Sep 8 10:35:20 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Sun, 08 Sep 2019 15:35:20 +0000 Subject: [Histonet] Tissue scrolling In-Reply-To: References: Message-ID: The best I can offer is to advise you to wipe the knife holder area down with a cleaning solvent and use a fresh blade. Clean forceps with a solvent and place the scroll (50 microns) directly into a labelled 1.5mL plastic vial. I would be interested on other thoughts on this. John www.ciqc.ca ??????? Original Message ??????? On Friday, September 6, 2019 3:05 PM, Erin McCarthy via Histonet wrote: > Hello Histonetters, > > I am curious if any of you perform scrolling of paraffin embedded tissue > for molecular studies? If so, what precautions do you take to minimize > contamination? I am asking specifically about at the cutting station as > that is my area of expertise. But we are looking to pilot this for samples > that in the past have had large quantities of recuts. > > Thank you for any information you can provide! > > ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- > > Erin McCarthy, HT (ASCP) > Histology Supervisor > > Tempus Labs > 600 W. Chicago Ave. > Chicago IL 60654 > Cell: (708)269-8610 > > ---------------------------------------------------------------------------------------------------------------------- > > This email and any attachments may contain privileged and confidential > information and/or protected health information (PHI) that is protected by > federal and state privacy laws.? It is intended solely for the use of > Tempus Labs and the recipient(s) named above.? Nothing contained in this > communication and any attachments thereto is intended to waive any > privileges or rights of confidentiality.? If you are not the recipient, or > the employee or agent responsible for delivering this message to the > intended recipient, you are hereby notified that any review, dissemination, > distribution, printing or copying of this email message and/or any > attachments is strictly prohibited.?* If you have received this > transmission in error, please notify us immediately at?(855)-442-8305? > and permanently delete this email and any attachments*. > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster at umn.edu Sun Sep 8 11:43:49 2019 From: cforster at umn.edu (Colleen Forster) Date: Sun, 8 Sep 2019 11:43:49 -0500 Subject: [Histonet] Tissue scrolling In-Reply-To: References: Message-ID: We cut alot if these. This is the protocol: 1. The microtome, blade and water bath are cleaned well. We follow withRNAse Away wipe down as an added precaution. 2. We use a coplin jar of 100% ethanol for forceps, pick or whatever other tool you might use that comes into contact with and dip and clean in between each sample. I actually have 2 sets and put them into the alcohol and switch out each time. 3 I use the RNAse free tubes to put the curls into. 4. You must wear gloves. 5. If they don't pick the samples up right away they are placed in a -20 freezer for storage. They have good results. Respectfully, Colleen Forster HT(ASCP) U of MN On Sun, Sep 8, 2019, 10:36 AM John Garratt via Histonet < histonet at lists.utsouthwestern.edu> wrote: > The best I can offer is to advise you to wipe the knife holder area down > with a cleaning solvent and use a fresh blade. Clean forceps with a solvent > and place the scroll (50 microns) directly into a labelled 1.5mL plastic > vial. I would be interested on other thoughts on this. > > John > > > > www.ciqc.ca > > > > > ??????? Original Message ??????? > On Friday, September 6, 2019 3:05 PM, Erin McCarthy via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hello Histonetters, > > > > I am curious if any of you perform scrolling of paraffin embedded tissue > > for molecular studies? If so, what precautions do you take to minimize > > contamination? I am asking specifically about at the cutting station as > > that is my area of expertise. But we are looking to pilot this for > samples > > that in the past have had large quantities of recuts. > > > > Thank you for any information you can provide! > > > > > ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- > > > > Erin McCarthy, HT (ASCP) > > Histology Supervisor > > > > Tempus Labs > > 600 W. Chicago Ave. > > Chicago IL 60654 > > Cell: (708)269-8610 > > > > > ---------------------------------------------------------------------------------------------------------------------- > > > > This email and any attachments may contain privileged and confidential > > information and/or protected health information (PHI) that is protected > by > > federal and state privacy laws. It is intended solely for the use of > > Tempus Labs and the recipient(s) named above. Nothing contained in this > > communication and any attachments thereto is intended to waive any > > privileges or rights of confidentiality. If you are not the recipient, > or > > the employee or agent responsible for delivering this message to the > > intended recipient, you are hereby notified that any review, > dissemination, > > distribution, printing or copying of this email message and/or any > > attachments is strictly prohibited. * If you have received this > > transmission in error, please notify us immediately at (855)-442-8305 > > and permanently delete this email and any attachments*. > > > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From naira.margaryan at hsc.wvu.edu Sun Sep 8 12:03:44 2019 From: naira.margaryan at hsc.wvu.edu (Margaryan, Naira) Date: Sun, 8 Sep 2019 17:03:44 +0000 Subject: [Histonet] Tissue scrolling In-Reply-To: References: , Message-ID: I do the same way! Good luck! Naira ________________________________ From: Colleen Forster Sent: Sunday, September 8, 2019 12:43 PM To: John Garratt Cc: Erin McCarthy ; histonet-request Subject: Re: [Histonet] Tissue scrolling We cut alot if these. This is the protocol: 1. The microtome, blade and water bath are cleaned well. We follow withRNAse Away wipe down as an added precaution. 2. We use a coplin jar of 100% ethanol for forceps, pick or whatever other tool you might use that comes into contact with and dip and clean in between each sample. I actually have 2 sets and put them into the alcohol and switch out each time. 3 I use the RNAse free tubes to put the curls into. 4. You must wear gloves. 5. If they don't pick the samples up right away they are placed in a -20 freezer for storage. They have good results. Respectfully, Colleen Forster HT(ASCP) U of MN On Sun, Sep 8, 2019, 10:36 AM John Garratt via Histonet < histonet at lists.utsouthwestern.edu> wrote: > The best I can offer is to advise you to wipe the knife holder area down > with a cleaning solvent and use a fresh blade. Clean forceps with a solvent > and place the scroll (50 microns) directly into a labelled 1.5mL plastic > vial. I would be interested on other thoughts on this. > > John > > > > www.ciqc.ca > > > > > ??????? Original Message ??????? > On Friday, September 6, 2019 3:05 PM, Erin McCarthy via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hello Histonetters, > > > > I am curious if any of you perform scrolling of paraffin embedded tissue > > for molecular studies? If so, what precautions do you take to minimize > > contamination? I am asking specifically about at the cutting station as > > that is my area of expertise. But we are looking to pilot this for > samples > > that in the past have had large quantities of recuts. > > > > Thank you for any information you can provide! > > > > > ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- > > > > Erin McCarthy, HT (ASCP) > > Histology Supervisor > > > > Tempus Labs > > 600 W. Chicago Ave. > > Chicago IL 60654 > > Cell: (708)269-8610 > > > > > ---------------------------------------------------------------------------------------------------------------------- > > > > This email and any attachments may contain privileged and confidential > > information and/or protected health information (PHI) that is protected > by > > federal and state privacy laws. It is intended solely for the use of > > Tempus Labs and the recipient(s) named above. Nothing contained in this > > communication and any attachments thereto is intended to waive any > > privileges or rights of confidentiality. If you are not the recipient, > or > > the employee or agent responsible for delivering this message to the > > intended recipient, you are hereby notified that any review, > dissemination, > > distribution, printing or copying of this email message and/or any > > attachments is strictly prohibited. * If you have received this > > transmission in error, please notify us immediately at (855)-442-8305 > > and permanently delete this email and any attachments*. > > > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From john.garratt at ciqc.ca Sun Sep 8 22:57:38 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Mon, 09 Sep 2019 03:57:38 +0000 Subject: [Histonet] Immunohistochemistry Workshop in Vancouver Message-ID: For technologists, pathologists, residents, scientists or anyone else interested in immunohistochemistry. On Friday, October 25, 2019, the cIQc will be holding our 2019 Technical Workshop: The Finer Details of IHC Readout. Click here to [REGISTER](https://pathology-payment.med.ubc.ca/2019-ciqc-technical-workshop/) - Based on the webinar series ?Epitopically Speaking?? learn the important difference between reading and interpreting IHC staining in tissues. - Learn how to design, implement and interpret the results of iCAPCs (immunohistochemistry Critical Assay Performance Controls ) to improve consistency and quality of staining in your laboratory. - See a demonstration of the new online IHC readout proficiency modules to be offered by the cIQc/CAP-ACP that will provide on-going CME to technologists and pathologists. Participants at the workshop will receive free registration for one module of choice. - Learn how to design and maintain a tissue control bank for IHC using four normal tissues that will cover the majority of your Class I testing. Vancouver Eye Care Centre, 2550 Willow Street, Vancouver, BC, Canada www.ciqc.ca From relia1 at earthlink.net Mon Sep 9 08:51:01 2019 From: relia1 at earthlink.net (relia1) Date: Mon, 09 Sep 2019 09:51:01 -0400 Subject: [Histonet] Histology Instructor needed in Orlando, FL A RELIA Exclusive! Message-ID: Hi Histonetters!I hope this is the start to a great week for you!I'm hoping you can help me.?I have been engaged by our local histology program to recruit an instructor.?If you are ASCP certified and are eligible for Florida licensure as a technologist and would like to get off the bench to instruct and inspire the next generation of histotechs we need to talk. OR? If you know anyone else who might be interested please contact me asap.??email: relia1 at earthlink.net?Cell 407-353-5070Toll free 866-607-3542#jobs4myhistopeeps?#ilovemyhistopeeps?Thanks-PamRight place Right time Right move with RELIA!Thank youPam Barker?RELIA Solutions?Cell 407-353-5070#jobs4myhistopeeps?#ilovemyhistopeeps?Sent from my Sprint Samsung Galaxy S10+. From mward at wakehealth.edu Mon Sep 9 10:04:32 2019 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Mon, 9 Sep 2019 15:04:32 +0000 Subject: [Histonet] PPE for the Histology lab Message-ID: Our department is looking at safety issues and one suggestion from our CLIA director is that everyone in the lab (our lab performs IHC only) should wear gloves, a gown and eye or face protection at all times and requiring it for anyone that enters the lab. We have task specific PPE requirements for these items to be worn when changing solutions on the stainers or working with serum for our indirect immunofluorescence for example but don't require it for FFPE microtomy or loading the IHC stainers. What are others requiring in their labs? Thanks in advance for your feedback on this issue. Martha Ward Manager, Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157 From Timothy.Morken at ucsf.edu Mon Sep 9 10:42:17 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 9 Sep 2019 15:42:17 +0000 Subject: [Histonet] PPE for the Histology lab In-Reply-To: References: Message-ID: Martha, that is an extreme view. Eye and face protection in pathology are primarily for splash protection from either chemicals or body fluids. I can't see any need for people sectioning, embedding, etc. Maybe for special stains. Yes for pouring a lot of chemicals. And the idea that anyone visiting the lab, not doing any of the work, always needing eye and gown protection is not necessary unless they are observing work with possible fluid splash or other kind of exposure. And then I would expect to inform them of whatever danger they are in by exposure. What you need to do is a risk assessment of each task and how likely various hazards are for each task, then determine what protections are needed. When we have issues like this we call in our Environmental Health and Safety department to assess the issue. They are good about keeping things practical. We recently had them assess liquid nitrogen use in our lab after CAP came out with some recommendations about needing oxygen sensors when using liquid nitrogen that spooked our director. They came in with oxygen sensors and determined we have plenty of ventilation and don't need them. At the same time they reviewed all our use of liquid nitrogen and passed off on our procedures and made a few practical recommendations. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Martha Ward-Pathology via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, September 09, 2019 8:05 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] PPE for the Histology lab Our department is looking at safety issues and one suggestion from our CLIA director is that everyone in the lab (our lab performs IHC only) should wear gloves, a gown and eye or face protection at all times and requiring it for anyone that enters the lab. We have task specific PPE requirements for these items to be worn when changing solutions on the stainers or working with serum for our indirect immunofluorescence for example but don't require it for FFPE microtomy or loading the IHC stainers. What are others requiring in their labs? Thanks in advance for your feedback on this issue. Martha Ward Manager, Molecular Diagnostics Lab Wake Forest Baptist Health Winston-Salem, NC 27157 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=7TeYoCPhmsjTKa4DAOQEneymmwin7fpnf60fen3QpSk&s=83KmtH4cZY3LhOgJpQh4gKwx6fIMBCcLOj8Ao7d3QMo&e= From BMolinari at texasheart.org Mon Sep 9 10:53:38 2019 From: BMolinari at texasheart.org (Betsy Molinari) Date: Mon, 9 Sep 2019 15:53:38 +0000 Subject: [Histonet] Mallory-Azan stain References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.11494c40-55bd-4e6f-ae0d-a75d91726f23@emailsignatures365.codetwo.com> Message-ID: Hi histonetters, I have a researcher that wants to stain Purkinje fibers and has requested a Mallory-Azan stain. I have no experience with this stain. I have looked online for information but am reaching out to you for personal advice. Thanks. Betsy Molinari HT,ASCP Texas Heart Institute 6770 Bertner Ave. Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter From rsrichmond at gmail.com Mon Sep 9 12:24:07 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Mon, 9 Sep 2019 13:24:07 -0400 Subject: [Histonet] Mallory-Azan stain In-Reply-To: References: Message-ID: Betsy Molinari HT, ASCP at the Texas Heart Institute in Houston asks: >>I have a researcher that wants to stain Purkinje fibers and has requested a Mallory-Azan stain. I have no experience with this stain. I have looked online for information but am reaching out to you for personal advice.<< Well, I'm 80 years old and have never seen a Mallory-Heidenhain azan stain - it was something you read about in old books when I was young. I have a fair collection of old books, and found a method in Ann Preece's venerable _A Manual for Histologic Technicians_, 3rd ed, 1972. I can scan it for you if nobody else sends you one. The stain requires aniline ("aniline oil"), which I don't think you're allowed to handle any more - it's a significant hazmat. Your researcher is probably using a very old reference, and needs to be brought into the 21st century. Bob Richmond Samurai Pathologist Maryville TN From lubbockcat at mail.com Mon Sep 9 17:28:08 2019 From: lubbockcat at mail.com (Michael Bradley) Date: Tue, 10 Sep 2019 00:28:08 +0200 Subject: [Histonet] Biocare Message-ID: Hi all I heard a rumor that Biocare is going out of business. Can anyone confirm or deny this? Thanks. Sent using the mail.com mail app From philip_manfre at merck.com Tue Sep 10 06:57:12 2019 From: philip_manfre at merck.com (Manfre, Philip) Date: Tue, 10 Sep 2019 11:57:12 +0000 Subject: [Histonet] Mallory-Azan stain Message-ID: The Bielschowsky Silver Stain is a good stain for Purkinje cells, so perhaps fibers as well. Phil. Philip Manfre, BA, HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP81-406 770 Sumneytown Pike West Point, PA 19486 215-652-9750 philip_manfre at merck.com -----Original Message----- From: Bob Richmond via Histonet Sent: Monday, September 9, 2019 1:24 PM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Mallory-Azan stain EXTERNAL EMAIL ? Use caution with any links or file attachments. Betsy Molinari HT, ASCP at the Texas Heart Institute in Houston asks: >>I have a researcher that wants to stain Purkinje fibers and has >>requested a Mallory-Azan stain. I have no experience with this stain. I have looked online for information but am reaching out to you for personal advice.<< Well, I'm 80 years old and have never seen a Mallory-Heidenhain azan stain - it was something you read about in old books when I was young. I have a fair collection of old books, and found a method in Ann Preece's venerable _A Manual for Histologic Technicians_, 3rd ed, 1972. I can scan it for you if nobody else sends you one. The stain requires aniline ("aniline oil"), which I don't think you're allowed to handle any more - it's a significant hazmat. Your researcher is probably using a very old reference, and needs to be brought into the 21st century. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. From BMolinari at texasheart.org Tue Sep 10 08:34:22 2019 From: BMolinari at texasheart.org (Betsy Molinari) Date: Tue, 10 Sep 2019 13:34:22 +0000 Subject: [Histonet] Mallory-Azan stain In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.4d542ea7-108a-4b2a-b66a-5a85f94689e2@emailsignatures365.codetwo.com> Message-ID: Betsy Molinari Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter -----Original Message----- From: Manfre, Philip via Histonet Sent: Tuesday, September 10, 2019 6:57 AM To: Bob Richmond ; Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Mallory-Azan stain *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ The Bielschowsky Silver Stain is a good stain for Purkinje cells, so perhaps fibers as well. Phil. Philip Manfre, BA, HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP81-406 770 Sumneytown Pike West Point, PA 19486 215-652-9750 philip_manfre at merck.com -----Original Message----- From: Bob Richmond via Histonet Sent: Monday, September 9, 2019 1:24 PM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Mallory-Azan stain EXTERNAL EMAIL ? Use caution with any links or file attachments. Betsy Molinari HT, ASCP at the Texas Heart Institute in Houston asks: >>I have a researcher that wants to stain Purkinje fibers and has >>requested a Mallory-Azan stain. I have no experience with this stain. I have looked online for information but am reaching out to you for personal advice.<< Well, I'm 80 years old and have never seen a Mallory-Heidenhain azan stain - it was something you read about in old books when I was young. I have a fair collection of old books, and found a method in Ann Preece's venerable _A Manual for Histologic Technicians_, 3rd ed, 1972. I can scan it for you if nobody else sends you one. The stain requires aniline ("aniline oil"), which I don't think you're allowed to handle any more - it's a significant hazmat. Your researcher is probably using a very old reference, and needs to be brought into the 21st century. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=JS3idhca79EooP3c3KHiszWriFXnRPpqN9stNIHee7E&s=IybChKWtsgzm4YnXGc1dkyQJZpndvz0FJR4LKjwzr-Y&e= Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at https://urldefense.proofpoint.com/v2/url?u=http-3A__www.merck.com_contact_contacts.html&d=DwIGaQ&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=JS3idhca79EooP3c3KHiszWriFXnRPpqN9stNIHee7E&s=-Su_YkQUKkXgI0vzm-t3bCyMxTKSX2LAL1AcP2fhaqo&e= ) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=JS3idhca79EooP3c3KHiszWriFXnRPpqN9stNIHee7E&s=IybChKWtsgzm4YnXGc1dkyQJZpndvz0FJR4LKjwzr-Y&e= From jhill at vet.k-state.edu Tue Sep 10 09:56:30 2019 From: jhill at vet.k-state.edu (Jennifer Phinney) Date: Tue, 10 Sep 2019 14:56:30 +0000 Subject: [Histonet] Detecting copper in H&E staining? Message-ID: Hello all, I am in a veterinary histology lab. One of my Pathologists mentioned that at two of the locations he'd been at previously (Ithaca, NY and Saskatoon, Canada) copper deposits stained in the routine H&E slides. Is anyone aware of how this could have been achieved? I assume it would either have to be an additive in the eosin or perhaps something naturally occurring in the tap water rinses? Thanks for any assistance, Jennifer Phinney QIHC Histology Laboratory Administrator KSVDL From rjbuesa at yahoo.com Tue Sep 10 10:32:09 2019 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 10 Sep 2019 15:32:09 +0000 (UTC) Subject: [Histonet] Detecting copper in H&E staining? In-Reply-To: References: Message-ID: <1215558422.5058371.1568129529009@mail.yahoo.com> Unfortunately your pathologist is wrong. You will need the "rhodamine" special IHC method to identify copper in tissue sections.Ren? On Tuesday, September 10, 2019, 11:08:09 AM EDT, Jennifer Phinney via Histonet wrote: Hello all, I am in a veterinary histology lab. One of my Pathologists mentioned that at two of the locations he'd been at previously (Ithaca, NY and Saskatoon, Canada) copper deposits stained in the routine H&E slides. Is anyone aware of how this could have been achieved? I assume it would either have to be an additive in the eosin or perhaps something naturally occurring in the tap water rinses? Thanks for any assistance, Jennifer Phinney QIHC Histology Laboratory Administrator KSVDL _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brettmc31 at comcast.net Tue Sep 10 10:39:30 2019 From: brettmc31 at comcast.net (Brett Connolly) Date: Tue, 10 Sep 2019 11:39:30 -0400 Subject: [Histonet] Detecting copper in H&E staining? In-Reply-To: <1215558422.5058371.1568129529009@mail.yahoo.com> References: <1215558422.5058371.1568129529009@mail.yahoo.com> Message-ID: I believe it is a rhoda?N?ine stain not rhoda?M?ine. I did it years ago on Alzheimer?s brain sections ? works great. http://www.ihcworld.com/_protocols/special_stains/rhodanine_ellis.htm Brett Connolly, Phd, HTL(ASCP) happily retired Sent from Mail for Windows 10 From: Rene J Buesa via Histonet Sent: Tuesday, September 10, 2019 11:32 AM To: HistoNet; Jennifer Phinney Subject: Re: [Histonet] Detecting copper in H&E staining? Unfortunately your pathologist is wrong. You will need the "rhodamine" special IHC method to identify copper in tissue sections.Ren? On Tuesday, September 10, 2019, 11:08:09 AM EDT, Jennifer Phinney via Histonet wrote: Hello all, I am in a veterinary histology lab. One of my Pathologists mentioned that at two of the locations he'd been at previously (Ithaca, NY and Saskatoon, Canada) copper deposits stained in the routine H&E slides. Is anyone aware of how this could have been achieved? I assume it would either have to be an additive in the eosin or perhaps something naturally occurring in the tap water rinses? Thanks for any assistance, Jennifer Phinney QIHC Histology Laboratory Administrator KSVDL _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan at uwo.ca Tue Sep 10 10:42:31 2019 From: jkiernan at uwo.ca (John Kiernan) Date: Tue, 10 Sep 2019 15:42:31 +0000 Subject: [Histonet] Mallory-Azan stain In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.11494c40-55bd-4e6f-ae0d-a75d91726f23@emailsignatures365.codetwo.com>, Message-ID: Mallory's (easy) and AZAN staining (difficult) are different methods! Frank B. Mallory's trichrome stain (Journal of Medical Research 13: 113-136, 1905) is the earliest and one of the simplest of its kind: acid fuchsine followed by a solution containing orange G, aniline blue and phosphotungstic acid (PTA). Martin Heidenhain's trichrome is usually called AZAN (from Azokarmin and Anilinblau, the German names of two of the dyes used. I've not read his original 1916 publiication, but a very thorough account was given by Manfred Gabe in his 1976 Histological Techniques book (ISBN 3540901620), pp. 219-223. I used this quite a bit in the 1990s mostly on paraffin sections of Bouin-fixed decalcified rats' heads. It is a 15-step procedure taking >2 hours and it includes two critical differentiations requiring careful microscopic control. Instructions based on my experiences can be found in Histological and Histochemical Methods (5th ed., 2015, pp.198-200). AZAN gives a wider range of colours than Mallory's or Masson's trichrome or the various one-step trichromes (Cason, Gomori, Gabe). The related Romeis "cresazan" procedure was used to identify at least 6 anterior pituitary cell-types until the 1950s when more rational histochemically based stains were introduced by Adams, Herlant, Pearse and others. Nowadays, immunostainng accurately shows the hormones in pituitary cells, but much more expensively. All trichromes give poor results after simple fixation in neutral formaldehyde. Bouin or (better) a mercuric chloride-containing fixative is needed. Zinc-formalin is probably also OK. (I haven't tried it myself for this purpose). If material fixed in NBF must be used, immerse hydrated paraffin sections in saturated aqueous picric acid either for 2h at 56-60C or overnight at room temperature, then wash well in water before staining. (Bouin's fluid is often used, but its ingredients other than picric acid are unnecessary.) Experiments are needed to learn the mechanism of this "rescue" of staining properties of sections formaldehyde-fixed tissue, which is sometimes wrongly called "mordanting". My guess is that it's comparable to antigen retrieval. It has been claimed that citrate buffer is just as good, though the photos are unconvincing (J. Histotechnol. 26, 133). It should be possible to identify Purkinje fibres with any staining method that shows nuclei and myofibrils, such as H&E or a trichrome method simpler than AZAN. A glycogen stain such as PAS might show this substance in the otherwise pale areas around the central nuclei of Purkinje fibres. I suggest persuading your researcher to let you try something simpler before attempting Heidenhain's AZAN. Wheater's Functional Histology has a nice photomicrograph of a section stained with H&E and for endocardial elastin (looks like orcein). Enough rambling! John Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada = = = ________________________________ From: Betsy Molinari via Histonet Sent: 09 September 2019 10:53 To: 'Histonet at lists.utsouthwestern.edu' Subject: [Histonet] Mallory-Azan stain Hi histonetters, I have a researcher that wants to stain Purkinje fibers and has requested a Mallory-Azan stain. I have no experience with this stain. I have looked online for information but am reaching out to you for personal advice. Thanks. Betsy Molinari HT,ASCP Texas Heart Institute 6770 Bertner Ave. Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Tue Sep 10 10:54:48 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 10 Sep 2019 15:54:48 +0000 Subject: [Histonet] Mallory-Azan stain In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.11494c40-55bd-4e6f-ae0d-a75d91726f23@emailsignatures365.codetwo.com>, Message-ID: John, we love it when you "ramble!" It gives us an appreciation for the history and breadth of histo techniques. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: John Kiernan via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 10, 2019 8:43 AM To: 'Histonet at lists.utsouthwestern.edu'; Betsy Molinari Subject: Re: [Histonet] Mallory-Azan stain Mallory's (easy) and AZAN staining (difficult) are different methods! Frank B. Mallory's trichrome stain (Journal of Medical Research 13: 113-136, 1905) is the earliest and one of the simplest of its kind: acid fuchsine followed by a solution containing orange G, aniline blue and phosphotungstic acid (PTA). Martin Heidenhain's trichrome is usually called AZAN (from Azokarmin and Anilinblau, the German names of two of the dyes used. I've not read his original 1916 publiication, but a very thorough account was given by Manfred Gabe in his 1976 Histological Techniques book (ISBN 3540901620), pp. 219-223. I used this quite a bit in the 1990s mostly on paraffin sections of Bouin-fixed decalcified rats' heads. It is a 15-step procedure taking >2 hours and it includes two critical differentiations requiring careful microscopic control. Instructions based on my experiences can be found in Histological and Histochemical Methods (5th ed., 2015, pp.198-200). AZAN gives a wider range of colours than Mallory's or Masson's trichrome or the various one-step trichromes (Cason, Gomori, Gabe). The related Romeis "cresazan" procedure was used to identify at least 6 anterior pituitary cell-types until the 1950s when more rational histochemically based stains were introduced by Adams, Herlant, Pearse and others. Nowadays, immunostainng accurately shows the hormones in pituitary cells, but much more expensively. All trichromes give poor results after simple fixation in neutral formaldehyde. Bouin or (better) a mercuric chloride-containing fixative is needed. Zinc-formalin is probably also OK. (I haven't tried it myself for this purpose). If material fixed in NBF must be used, immerse hydrated paraffin sections in saturated aqueous picric acid either for 2h at 56-60C or overnight at room temperature, then wash well in water before staining. (Bouin's fluid is often used, but its ingredients other than picric acid are unnecessary.) Experiments are needed to learn the mechanism of this "rescue" of staining properties of sections formaldehyde-fixed tissue, which is sometimes wrongly called "mordanting". My guess is that it's comparable to antigen retrieval. It has been claimed that citrate buffer is just as good, though the photos are unconvincing (J. Histotechnol. 26, 133). It should be possible to identify Purkinje fibres with any staining method that shows nuclei and myofibrils, such as H&E or a trichrome method simpler than AZAN. A glycogen stain such as PAS might show this substance in the otherwise pale areas around the central nuclei of Purkinje fibres. I suggest persuading your researcher to let you try something simpler before attempting Heidenhain's AZAN. Wheater's Functional Histology has a nice photomicrograph of a section stained with H&E and for endocardial elastin (looks like orcein). Enough rambling! John Kiernan Anatomy & Cell Biology University of Western Ontario London, Canada = = = ________________________________ From: Betsy Molinari via Histonet Sent: 09 September 2019 10:53 To: 'Histonet at lists.utsouthwestern.edu' Subject: [Histonet] Mallory-Azan stain Hi histonetters, I have a researcher that wants to stain Purkinje fibers and has requested a Mallory-Azan stain. I have no experience with this stain. I have looked online for information but am reaching out to you for personal advice. Thanks. Betsy Molinari HT,ASCP Texas Heart Institute 6770 Bertner Ave. Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=gOhsr6cogN3TBZh8C2dHLiczk4rN5grOBAD1YkzJlrk&s=Tg-ltd7S0Gvp2_-XSvBjsRuw1esVa8scKHMk6uYj10c&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=gOhsr6cogN3TBZh8C2dHLiczk4rN5grOBAD1YkzJlrk&s=Tg-ltd7S0Gvp2_-XSvBjsRuw1esVa8scKHMk6uYj10c&e= From cforster at umn.edu Tue Sep 10 11:17:27 2019 From: cforster at umn.edu (Colleen Forster) Date: Tue, 10 Sep 2019 11:17:27 -0500 Subject: [Histonet] Mallory-Azan stain In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.11494c40-55bd-4e6f-ae0d-a75d91726f23@emailsignatures365.codetwo.com> Message-ID: Wow John, Great information.,....always so much to learn!~ Thank you, Colleen Forster U of MN On Tue, Sep 10, 2019 at 10:55 AM Morken, Timothy via Histonet < histonet at lists.utsouthwestern.edu> wrote: > John, we love it when you "ramble!" It gives us an appreciation for the > history and breadth of histo techniques. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > -----Original Message----- > From: John Kiernan via Histonet [mailto:histonet at lists.utsouthwestern.edu] > > Sent: Tuesday, September 10, 2019 8:43 AM > To: 'Histonet at lists.utsouthwestern.edu'; Betsy Molinari > Subject: Re: [Histonet] Mallory-Azan stain > > Mallory's (easy) and AZAN staining (difficult) are different methods! > > Frank B. Mallory's trichrome stain (Journal of Medical Research 13: > 113-136, 1905) is the earliest and one of the simplest of its kind: acid > fuchsine followed by a solution containing orange G, aniline blue and > phosphotungstic acid (PTA). Martin Heidenhain's trichrome is usually > called AZAN (from Azokarmin and Anilinblau, the German names of two of the > dyes used. I've not read his original 1916 publiication, but a very > thorough account was given by Manfred Gabe in his 1976 Histological > Techniques book (ISBN 3540901620), pp. 219-223. I used this quite a bit in > the 1990s mostly on paraffin sections of Bouin-fixed decalcified rats' > heads. It is a 15-step procedure taking >2 hours and it includes two > critical differentiations requiring careful microscopic control. > Instructions based on my experiences can be found in Histological and > Histochemical Methods (5th ed., 2015, pp.198-200). > > AZAN gives a wider range of colours than Mallory's or Masson's trichrome > or the various one-step trichromes (Cason, Gomori, Gabe). The related > Romeis "cresazan" procedure was used to identify at least 6 anterior > pituitary cell-types until the 1950s when more rational histochemically > based stains were introduced by Adams, Herlant, Pearse and others. > Nowadays, immunostainng accurately shows the hormones in pituitary cells, > but much more expensively. > > All trichromes give poor results after simple fixation in neutral > formaldehyde. Bouin or (better) a mercuric chloride-containing fixative is > needed. Zinc-formalin is probably also OK. (I haven't tried it myself for > this purpose). If material fixed in NBF must be used, immerse hydrated > paraffin sections in saturated aqueous picric acid either for 2h at 56-60C > or overnight at room temperature, then wash well in water before staining. > (Bouin's fluid is often used, but its ingredients other than picric acid > are unnecessary.) Experiments are needed to learn the mechanism of this > "rescue" of staining properties of sections formaldehyde-fixed tissue, > which is sometimes wrongly called "mordanting". My guess is that it's > comparable to antigen retrieval. It has been claimed that citrate buffer is > just as good, though the photos are unconvincing (J. Histotechnol. 26, 133). > > It should be possible to identify Purkinje fibres with any staining method > that shows nuclei and myofibrils, such as H&E or a trichrome method simpler > than AZAN. A glycogen stain such as PAS might show this substance in the > otherwise pale areas around the central nuclei of Purkinje fibres. I > suggest persuading your researcher to let you try something simpler before > attempting Heidenhain's AZAN. Wheater's Functional Histology has a nice > photomicrograph of a section stained with H&E and for endocardial elastin > (looks like orcein). > > Enough rambling! > John Kiernan > Anatomy & Cell Biology > University of Western Ontario > London, Canada > = = = > > ________________________________ > From: Betsy Molinari via Histonet > Sent: 09 September 2019 10:53 > To: 'Histonet at lists.utsouthwestern.edu' > > Subject: [Histonet] Mallory-Azan stain > > Hi histonetters, > I have a researcher that wants to stain Purkinje fibers and has requested > a Mallory-Azan stain. > I have no experience with this stain. I have looked online for information > but am reaching out to you for personal advice. > Thanks. > Betsy Molinari HT,ASCP > Texas Heart Institute > 6770 Bertner Ave. > Houston, TX 77030 > 832-355-6524 (lab) > 832-355-6812 (fax) > > Betsy Molinari > Sr. Histology Research Technician > CV Pathology Research > > Texas Heart Institute > 6770 Bertner Avenue, MC 1-283 > Houston, TX 77030 > > Office: 832-355-6524 | Fax: 832-355-6812 > Email: BMolinari at texasheart.org > texasheart.org< > https://urldefense.proofpoint.com/v2/url?u=https-3A__www.texasheart.org_&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=gOhsr6cogN3TBZh8C2dHLiczk4rN5grOBAD1YkzJlrk&s=8BI3BsDaT4RpUA7gRKKiLai8Lsjj8dijlm_QRu3kElg&e= > > | facebook< > https://urldefense.proofpoint.com/v2/url?u=https-3A__www.facebook.com_Texas.Heart.Institute&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=gOhsr6cogN3TBZh8C2dHLiczk4rN5grOBAD1YkzJlrk&s=deGJye4k3LKtsaLIxFu7_k3FNXU5R6uqcgIRXry7oug&e= > > | twitter< > https://urldefense.proofpoint.com/v2/url?u=https-3A__twitter.com_Texas-5FHeart&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=gOhsr6cogN3TBZh8C2dHLiczk4rN5grOBAD1YkzJlrk&s=r7bgnhb3v6jPkFz2Km9Nf_p5ltR1MFAjWKurvn82-GI&e= > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=gOhsr6cogN3TBZh8C2dHLiczk4rN5grOBAD1YkzJlrk&s=Tg-ltd7S0Gvp2_-XSvBjsRuw1esVa8scKHMk6uYj10c&e= > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=gOhsr6cogN3TBZh8C2dHLiczk4rN5grOBAD1YkzJlrk&s=Tg-ltd7S0Gvp2_-XSvBjsRuw1esVa8scKHMk6uYj10c&e= > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at holml at umn.edu * From cristante08 at gmail.com Tue Sep 10 12:26:37 2019 From: cristante08 at gmail.com (cristante08 at gmail.com) Date: Tue, 10 Sep 2019 13:26:37 -0400 Subject: [Histonet] BioCare Message-ID: <2CA417E2-7E13-4AD1-A392-AD577691E72F@gmail.com> Howdy HistoNet!!! I seen a post about a rumor regarding BioCare going out of business. This rumor is false!!! BioCare continues to expand their selection of products and services. Stop by their booth at NSH in New Orleans!! Brandy Cristante From penny.marr at nhs.net Wed Sep 11 02:21:10 2019 From: penny.marr at nhs.net (MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST)) Date: Wed, 11 Sep 2019 07:21:10 +0000 Subject: [Histonet] Detecting copper in H&E staining? In-Reply-To: References: Message-ID: Perhaps they did an orcein stain with H&E counterstain? That would make the copper associated protein a dark red-brown colour but with other components looking like an H&E. Penny Marr Senior BMS C/- Histology Conquest Hospital St Leonards-on-Sea TN37 7RD penny.marr at nhs.net (01424) 758023 -----Original Message----- From: Jennifer Phinney [mailto:jhill at vet.k-state.edu] Sent: 10 September 2019 15:57 To: HistoNet Subject: [Histonet] Detecting copper in H&E staining? Hello all, I am in a veterinary histology lab. One of my Pathologists mentioned that at two of the locations he'd been at previously (Ithaca, NY and Saskatoon, Canada) copper deposits stained in the routine H&E slides. Is anyone aware of how this could have been achieved? I assume it would either have to be an additive in the eosin or perhaps something naturally occurring in the tap water rinses? Thanks for any assistance, Jennifer Phinney QIHC Histology Laboratory Administrator KSVDL ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in relation to its contents. To do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland. NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and other accredited email services. For more information and to find out how you can switch, https://portal.nhs.net/help/joiningnhsmail From Timothy.Morken at ucsf.edu Wed Sep 11 10:58:00 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 11 Sep 2019 15:58:00 +0000 Subject: [Histonet] A little temperture monitoring debate Message-ID: We had our Joint Commission inspection today (no deficiencies!) and the inspector was pressing us to do temperature monitoring in a different way. For chemicals in fridges and freezers we use the manufacturer recommendation of temperature range to set our ranges. Typically for a fridge it is 2C to 8C. That seems pretty standard from datasheets I have. So we set our range at 2-8 on our automated system and then have a 30 min delay if it goes over before an alert sounds. That is to prevent spurious out of range alerts just because someone opened the door for a bit longer than usual. And reagents are not going to warm up instantly either. The probe is in a liquid bottle so will warm up time will be similar to a reagent. Anyway, he thought we should set the ranges narrower so it alerts before it reaches the out of range mark. He felt that if the fridge goes out of range for any time AT ALL then we need to prove all the reagents are still good. He was satisfied with daily control review of immuno stains and said that would prove the reagents work. But I also pointed out to him that we take the diluted reagents out of the fridge and have them on the stainer for up to 8 hours every day at room temperature with no problems. He didn't really have an answer to that but said the manufacturer should consider that in their literature. We don't have too many alerts and those that do occur are usually due to a door not closed are resolved quickly. I'm wondering what others think and do. We had debate this internally when we set up the automated system and considered wider ranges to avoid too many out of range alerts due to opening the doors many times daily, but never considered narrower ranges. We decided to go with the manufacturer ranges in order to be consistent and not have to defend whatever arbitrary narrow or wide range we picked. At least with the manufacturer recommendations we have something on paper to point to. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From Jessica.Piche at wtbyhosp.org Wed Sep 11 13:05:17 2019 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Wed, 11 Sep 2019 18:05:17 +0000 Subject: [Histonet] pan-TRK or NTRK antibody Message-ID: Good Afternoon, Where are people buying the pan-TRK or NTRK antibody from? Pre-dilute preferred. Thank you in advance and have a great day! Jessica Piche, HT(ASCP) Waterbury Hosptial Waterbury, CT 06708 From BMolinari at texasheart.org Wed Sep 11 13:42:14 2019 From: BMolinari at texasheart.org (Betsy Molinari) Date: Wed, 11 Sep 2019 18:42:14 +0000 Subject: [Histonet] Mallory-Azan stain In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.11494c40-55bd-4e6f-ae0d-a75d91726f23@emailsignatures365.codetwo.com> Message-ID: Thank you John, As always, a wealth of information. I did do a PAS for him, I am not totally sure he knows exactly what he is looking for. I always learn something from your posts. Betsy Betsy Molinari Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter -----Original Message----- From: Colleen Forster via Histonet Sent: Tuesday, September 10, 2019 11:17 AM To: Morken, Timothy Cc: Histonet ; John Kiernan Subject: Re: [Histonet] Mallory-Azan stain *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Wow John, Great information.,....always so much to learn!~ Thank you, Colleen Forster U of MN On Tue, Sep 10, 2019 at 10:55 AM Morken, Timothy via Histonet < histonet at lists.utsouthwestern.edu> wrote: > John, we love it when you "ramble!" It gives us an appreciation for > the history and breadth of histo techniques. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology UC San Francisco Medical Center > > -----Original Message----- > From: John Kiernan via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > > Sent: Tuesday, September 10, 2019 8:43 AM > To: 'Histonet at lists.utsouthwestern.edu'; Betsy Molinari > Subject: Re: [Histonet] Mallory-Azan stain > > Mallory's (easy) and AZAN staining (difficult) are different methods! > > Frank B. Mallory's trichrome stain (Journal of Medical Research 13: > 113-136, 1905) is the earliest and one of the simplest of its kind: > acid fuchsine followed by a solution containing orange G, aniline blue > and phosphotungstic acid (PTA). Martin Heidenhain's trichrome is > usually called AZAN (from Azokarmin and Anilinblau, the German names > of two of the dyes used. I've not read his original 1916 publiication, > but a very thorough account was given by Manfred Gabe in his 1976 > Histological Techniques book (ISBN 3540901620), pp. 219-223. I used > this quite a bit in the 1990s mostly on paraffin sections of Bouin-fixed decalcified rats' > heads. It is a 15-step procedure taking >2 hours and it includes two > critical differentiations requiring careful microscopic control. > Instructions based on my experiences can be found in Histological and > Histochemical Methods (5th ed., 2015, pp.198-200). > > AZAN gives a wider range of colours than Mallory's or Masson's > trichrome or the various one-step trichromes (Cason, Gomori, Gabe). > The related Romeis "cresazan" procedure was used to identify at least > 6 anterior pituitary cell-types until the 1950s when more rational > histochemically based stains were introduced by Adams, Herlant, Pearse and others. > Nowadays, immunostainng accurately shows the hormones in pituitary > cells, but much more expensively. > > All trichromes give poor results after simple fixation in neutral > formaldehyde. Bouin or (better) a mercuric chloride-containing > fixative is needed. Zinc-formalin is probably also OK. (I haven't > tried it myself for this purpose). If material fixed in NBF must be > used, immerse hydrated paraffin sections in saturated aqueous picric > acid either for 2h at 56-60C or overnight at room temperature, then wash well in water before staining. > (Bouin's fluid is often used, but its ingredients other than picric > acid are unnecessary.) Experiments are needed to learn the mechanism > of this "rescue" of staining properties of sections > formaldehyde-fixed tissue, which is sometimes wrongly called > "mordanting". My guess is that it's comparable to antigen retrieval. > It has been claimed that citrate buffer is just as good, though the photos are unconvincing (J. Histotechnol. 26, 133). > > It should be possible to identify Purkinje fibres with any staining > method that shows nuclei and myofibrils, such as H&E or a trichrome > method simpler than AZAN. A glycogen stain such as PAS might show this > substance in the otherwise pale areas around the central nuclei of > Purkinje fibres. I suggest persuading your researcher to let you try > something simpler before attempting Heidenhain's AZAN. Wheater's > Functional Histology has a nice photomicrograph of a section stained > with H&E and for endocardial elastin (looks like orcein). > > Enough rambling! > John Kiernan > Anatomy & Cell Biology > University of Western Ontario > London, Canada > = = = > > ________________________________ > From: Betsy Molinari via Histonet > Sent: 09 September 2019 10:53 > To: 'Histonet at lists.utsouthwestern.edu' > > > Subject: [Histonet] Mallory-Azan stain > > Hi histonetters, > I have a researcher that wants to stain Purkinje fibers and has > requested a Mallory-Azan stain. > I have no experience with this stain. I have looked online for > information but am reaching out to you for personal advice. > Thanks. > Betsy Molinari HT,ASCP > Texas Heart Institute > 6770 Bertner Ave. > Houston, TX 77030 > 832-355-6524 (lab) > 832-355-6812 (fax) > > Betsy Molinari > Sr. Histology Research Technician > CV Pathology Research > > Texas Heart Institute > 6770 Bertner Avenue, MC 1-283 > Houston, TX 77030 > > Office: 832-355-6524 | Fax: 832-355-6812 > Email: BMolinari at texasheart.org > texasheart.org< > https://urldefense.proofpoint.com/v2/url?u=https-3A__www.texasheart.or > g_&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLL > Hm33agI_sCKA&m=gOhsr6cogN3TBZh8C2dHLiczk4rN5grOBAD1YkzJlrk&s=8BI3BsDaT > 4RpUA7gRKKiLai8Lsjj8dijlm_QRu3kElg&e= > > | facebook< > https://urldefense.proofpoint.com/v2/url?u=https-3A__www.facebook.com_ > Texas.Heart.Institute&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jD > X2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=gOhsr6cogN3TBZh8C2dHLiczk4rN5grOBAD1 > YkzJlrk&s=deGJye4k3LKtsaLIxFu7_k3FNXU5R6uqcgIRXry7oug&e= > > | twitter< > https://urldefense.proofpoint.com/v2/url?u=https-3A__twitter.com_Texas > -5FHeart&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlW > AfHgLLHm33agI_sCKA&m=gOhsr6cogN3TBZh8C2dHLiczk4rN5grOBAD1YkzJlrk&s=r7b > gnhb3v6jPkFz2Km9Nf_p5ltR1MFAjWKurvn82-GI&e= > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7 > cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=gOhsr6cogN3TBZh8C2dHLiczk > 4rN5grOBAD1YkzJlrk&s=Tg-ltd7S0Gvp2_-XSvBjsRuw1esVa8scKHMk6uYj10c&e= > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7 > cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=gOhsr6cogN3TBZh8C2dHLiczk > 4rN5grOBAD1YkzJlrk&s=Tg-ltd7S0Gvp2_-XSvBjsRuw1esVa8scKHMk6uYj10c&e= > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=DwICAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS > 2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=quNS > ZEFa2FVyi3sjkMmFRPYERTYMO5ZnZRElS2B965M&s=qJoSsI4vFqfWRb5CrF9UfAbfdd-Z > CjnwiTk38cLUpew&e= > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at holml at umn.edu * _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=quNSZEFa2FVyi3sjkMmFRPYERTYMO5ZnZRElS2B965M&s=qJoSsI4vFqfWRb5CrF9UfAbfdd-ZCjnwiTk38cLUpew&e= From marktarango at gmail.com Wed Sep 11 14:21:30 2019 From: marktarango at gmail.com (Mark Tarango) Date: Wed, 11 Sep 2019 12:21:30 -0700 Subject: [Histonet] pan-TRK or NTRK antibody In-Reply-To: References: Message-ID: While we're on the topic of NTRK, does anyone have a positive case that they could share? I'm working up the FISH and have probes for NTRK1, 2 & 3. Any pan-NTRK positive IHC case would be great for detecting by FISH too. thanks! Mark Tarango On Wed, Sep 11, 2019 at 11:23 AM Piche, Jessica via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good Afternoon, > > Where are people buying the pan-TRK or NTRK antibody from? Pre-dilute > preferred. Thank you in advance and have a great day! > > Jessica Piche, HT(ASCP) > Waterbury Hosptial > Waterbury, CT 06708 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From john.garratt at ciqc.ca Wed Sep 11 15:27:47 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Wed, 11 Sep 2019 20:27:47 +0000 Subject: [Histonet] pan-TRK or NTRK antibody In-Reply-To: References: Message-ID: VENTANA pan-TRK (EPR17341) Assay Catalog Number: 790-7026 It should not be too difficult to find positive tumour controls since there is a high prevalence of NTRK in some cancers. ie Papillary thyroid cancer and secretary cancers ie secretary breast cancer (75%) Normal testis should stain positive. Endothelial cells should stain positive and will make a great internal control. John Garratt www.ciqc.ca ??????? Original Message ??????? On Wednesday, September 11, 2019 12:21 PM, Mark Tarango via Histonet wrote: > While we're on the topic of NTRK, does anyone have a positive case that > they could share? I'm working up the FISH and have probes for NTRK1, 2 & > 3. Any pan-NTRK positive IHC case would be great for detecting by FISH too. > > thanks! > > Mark Tarango > > On Wed, Sep 11, 2019 at 11:23 AM Piche, Jessica via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Good Afternoon, > > Where are people buying the pan-TRK or NTRK antibody from? Pre-dilute > > preferred. Thank you in advance and have a great day! > > Jessica Piche, HT(ASCP) > > Waterbury Hosptial > > Waterbury, CT 06708 > > > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pablo.sanchez at usc.es Thu Sep 12 03:18:56 2019 From: pablo.sanchez at usc.es (SANCHEZ QUINTEIRO PABLO) Date: Thu, 12 Sep 2019 08:18:56 +0000 Subject: [Histonet] Anti-SMI 32 Message-ID: Dear listers, Have you recently purchased an antibody against SMI32 (Anti-Neurofilament H Non-Phosphorylated Mouse mAb) I was looking for one from Millipore, now Merck, now Sigma, or whatever, but it is not longer available. Could you suggest me an alternative? Thanks in advance. Pablo Sanchez From plucas at biopath.org Thu Sep 12 09:45:08 2019 From: plucas at biopath.org (Paula) Date: Thu, 12 Sep 2019 07:45:08 -0700 Subject: [Histonet] Chromogenic ACD RNA ISH probes Message-ID: <001701d56978$ad613a80$0823af80$@biopath.org> Hello everyone, We recently received informative handouts regarding this technology from our Leica rep and we are in the process of scheduling a meeting to learn more about it. They say it's so wonderful, our jaws will drop from how beautiful the stains are. Can anyone comment on this testing? Would it be a nice addition to our library of IHC tests? Any comments are welcomed! Thank you in advance, Paula Lab Manager for Bio-Path Medical Group From Richard.Cartun at hhchealth.org Thu Sep 12 10:09:51 2019 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Thu, 12 Sep 2019 15:09:51 +0000 Subject: [Histonet] Chromogenic ACD RNA ISH probes In-Reply-To: <001701d56978$ad613a80$0823af80$@biopath.org> References: <001701d56978$ad613a80$0823af80$@biopath.org> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EAC67DDF8@HHCEXCHMB03.hhcsystem.org> We are currently running RNAScope for HPV and Albumin on our Leica Bond IIIs. It's a wonderful technology! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org -----Original Message----- From: Paula via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 12, 2019 10:45 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Chromogenic ACD RNA ISH probes THIS email is from Outside HHC! BE CAREFUL when opening attachments or click links from unknown senders. Hello everyone, We recently received informative handouts regarding this technology from our Leica rep and we are in the process of scheduling a meeting to learn more about it. They say it's so wonderful, our jaws will drop from how beautiful the stains are. Can anyone comment on this testing? Would it be a nice addition to our library of IHC tests? Any comments are welcomed! Thank you in advance, Paula Lab Manager for Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=E-WYJcMx9jdIa0p_q-5zjdtbDEqRoQJzZoEbVbzdGbc&s=VsAW0b6kdEIk2R5Hk67LryzbvHNN9iPdxFz8lAbum_I&e= Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From melissa at alliedsearchpartners.com Thu Sep 12 11:45:17 2019 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Thu, 12 Sep 2019 16:45:17 +0000 Subject: [Histonet] Histotech Job in Syracuse, NY- Message-ID: Hello, I have a second and third shift Full Time/Permanent Histotech position in Syracuse, NY. Potential for relocation assistance. Please reach out to me directly for more details. Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners Direct (Call) Line: 386.265.1368 Text Me: 386.855.8758 (Don't Call/Just Text) Toll Free: 888.388.7571 ext. 102 Fax: 888.388.7572 From mcruz84pr at gmail.com Thu Sep 12 12:27:24 2019 From: mcruz84pr at gmail.com (Maria Cruz) Date: Thu, 12 Sep 2019 13:27:24 -0400 Subject: [Histonet] Fwd: [HISTONET] Biocare In-Reply-To: References: Message-ID: Mr. Bradley: This is how awful rumors get started. I?m sure that Biocare is not going out of business. They?ve recently released a new stainer and last weak an IVD-labeled antibody for P16. If a sales rep from another company told you this they are pretty unethical and could learn a few things from the people at Biocare. Maria ---------- Forwarded message ---------- From: Michael Bradley To: Histopeeps Histonet Cc: Bcc: Date: Tue, 10 Sep 2019 00:28:08 +0200 Subject: [Histonet] Biocare Hi all I heard a rumor that Biocare is going out of business. Can anyone confirm or deny this? Thanks. Sent using the mail.com mail app From cforster at umn.edu Thu Sep 12 12:37:02 2019 From: cforster at umn.edu (Colleen Forster) Date: Thu, 12 Sep 2019 12:37:02 -0500 Subject: [Histonet] Fwd: [HISTONET] Biocare In-Reply-To: References: Message-ID: I'd love to know who actually told Michael this? I've had a company try this on me as well....interested to know if it is the same company. C. Forster On Thu, Sep 12, 2019 at 12:27 PM Maria Cruz via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Mr. Bradley: > This is how awful rumors get started. I?m sure that Biocare is not going > out of business. They?ve recently released a new stainer and last weak an > IVD-labeled antibody for P16. If a sales rep from another company told you > this they are pretty unethical and could learn a few things from the people > at Biocare. > Maria > > ---------- Forwarded message ---------- > From: Michael Bradley > To: Histopeeps Histonet > Cc: > Bcc: > Date: Tue, 10 Sep 2019 00:28:08 +0200 > Subject: [Histonet] Biocare > > Hi all > > I heard a rumor that Biocare is going out of business. Can anyone confirm > or deny this? > > Thanks. > Sent using the mail.com > mail app > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 From katherine at ka-recruiting.com Thu Sep 12 12:43:39 2019 From: katherine at ka-recruiting.com (Katherine Marano) Date: Thu, 12 Sep 2019 13:43:39 -0400 Subject: [Histonet] Histology Job Openings Message-ID: Hi Histonetters, I hope you are enjoying the start of September! Did you know that this is one of the best times of year to start a new job? I work at K.A. Recruiting specializing in placing laboratory professionals into permanent, full-time positions nationwide, and I am currently working with many hospitals and labs that are looking to hire in their histology departments. My clients are offering competitive compensation and benefits, and many also assist with a sign-on bonus and relocation assistance!! Current openings include: Phoenix, AZ - Histotech Northern Florida - Histotech (2nd shift) Central Georgia - Histotech (3a-1130a) Chicago, IL - Histotech (midnight shift) Charlotte, NC - Histotech High Point, NC - HTT/ HT/HTL 3rd shift (10pm to 6:30am - Sun night to Fri morning) North Dakota - Histology Lead, Monday-Friday day shift Long Island, New York - Histotech White Plains, NY - Grossing Tech Syracuse, NY - Histotech Salem, OR- Histotech (M-F day shift, IHC) Nashville, TN - Grosser (evening/ night shift) Richmond, VA- Histotech Roanoke, VA - Histotech Manitowac, WI - Histotech (3rd shift but may be flexible) If you want to hear more, could you send me a resume and a good time to give you a quick call? I?d love to discuss these opportunities with you! Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From relia1 at earthlink.net Thu Sep 12 13:21:58 2019 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 12 Sep 2019 14:21:58 -0400 Subject: [Histonet] RELIA Solutions Histology Job Alert. Opportunities on and off the bench nationwide! Message-ID: <00fd01d56996$f7545e10$e5fd1a30$@earthlink.net> Hi Histonetters! I hope you are having a great week. Here are my latest and greatest job opportunities: Southern, CA Director of Customer Support and Training Chicago, IL Applications Specialist - IHC Southwestern U.S. Histology Sales Orlando, FL Histology Instructor Syracuse, NY Histotech - 1st Shift - Salem, VA Histotech - 1st Shift Eugene, OR Mohs Histotech Milwaukee, WI Histotech 1st Shift Stockton, CA Histotech - nights All of these positions are full time and permanent. Most of them are RELIA Exclusives and all of my clients offer excellent compensation and benefits and in most cases relo/sign on bonuses! If you or anyone you know would like more info on any of these positions or would like to subscribe to my histology careers bulletin for more detailed information just shoot me a reply back to this post with the words I'm in! Or Subscribe - your choice. If you are going to be in New Orleans next week for NSH please look for me at the registration desk and say hi! I love to meet new histopeeps and reconnect with my old friends. Have an amazing day!! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Blanca.Lopez at UTSouthwestern.edu Thu Sep 12 13:48:25 2019 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Thu, 12 Sep 2019 18:48:25 +0000 Subject: [Histonet] Help to interview new employees for the first time Message-ID: <973afa7d97ab413f9552b6fd7abe11a3@SWMS13MAIL10.swmed.org> I am going to interview people for Histotech position for the first time...what are the best questions to ask? How do I prepare myself? what is the I need to know that they are the best one? What should I ask or choose? Is good to put them in action like cutting or staining to check on their skills or what are your recommendations? thank you for your help Blanca Lopez HT (ASCP)cm Senior Histotechnologist UT Southwestern Medical Center Harold C. Simmons Comprehensive Cancer Center UTSTR Biorepository Tissue Lab 6000 Harry Hines Blvd NB5.102 Dallas, Texas 75390 214-648-7598 blanca.lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From Toni.Rathborne at RWJBH.org Thu Sep 12 13:49:21 2019 From: Toni.Rathborne at RWJBH.org (Rathborne, Toni) Date: Thu, 12 Sep 2019 18:49:21 +0000 Subject: [Histonet] Fwd: [HISTONET] Biocare In-Reply-To: References: Message-ID: I got this reply from my Biocare rep. We are not going out of business What happened is over a year ago we were acquired by a Venture group, which is a good thing. They infuse money and are making us stronger. ------------------------------------------------------------------------------------ NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. -----Original Message----- From: Colleen Forster via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 12, 2019 1:37 PM To: Maria Cruz Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Fwd: [HISTONET] Biocare *** This is an External Email *** I'd love to know who actually told Michael this? I've had a company try this on me as well....interested to know if it is the same company. C. Forster On Thu, Sep 12, 2019 at 12:27 PM Maria Cruz via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Mr. Bradley: > This is how awful rumors get started. I?m sure that Biocare is not going > out of business. They?ve recently released a new stainer and last weak an > IVD-labeled antibody for P16. If a sales rep from another company told you > this they are pretty unethical and could learn a few things from the people > at Biocare. > Maria > > ---------- Forwarded message ---------- > From: Michael Bradley > To: Histopeeps Histonet > Cc: > Bcc: > Date: Tue, 10 Sep 2019 00:28:08 +0200 > Subject: [Histonet] Biocare > > Hi all > > I heard a rumor that Biocare is going out of business. Can anyone confirm > or deny this? > > Thanks. > Sent using the mail.com > mail app > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=qNyceGRYNM7Hsa4aC7FIQrlWODVZ8apJdZ59lI77YzM&s=PdsiEeE0Poe3SZ4E5Uszual_S5eiuQL0MNvYUG_fdP4&e= > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=LfJFs5tz11XIvZ1zGnYRWYcpprcdQWHKbyr0OjT-Gjk&r=OywojvDeqnDOvbIWXIx1jW-8xZXD1RJBnKKp8Mh6i_g&m=qNyceGRYNM7Hsa4aC7FIQrlWODVZ8apJdZ59lI77YzM&s=PdsiEeE0Poe3SZ4E5Uszual_S5eiuQL0MNvYUG_fdP4&e= From erin.mccarthy at tempus.com Thu Sep 12 14:34:36 2019 From: erin.mccarthy at tempus.com (Erin McCarthy) Date: Thu, 12 Sep 2019 14:34:36 -0500 Subject: [Histonet] Help to interview new employees for the first time In-Reply-To: <973afa7d97ab413f9552b6fd7abe11a3@SWMS13MAIL10.swmed.org> References: <973afa7d97ab413f9552b6fd7abe11a3@SWMS13MAIL10.swmed.org> Message-ID: Hi Blanca, I think practical skills tests are a great idea. BUT I also think you need to make sure that having someone that isn't an employee doing tasks in the lab is a responsibility your lab would willingly take on if something were to happen. If the candidate cut themselves and was litigious they could go after your lab. I have not heard of it happening, but I know in this day and age it can be a risk. Otherwise I try to ask probing questions - usually things that cannot be answered with Yes or No. Also, I try to ask about things you really want to know - how do they manage stress, do they have troubleshooting experience, if so can they explain the problem and how they solved it. Are they intuitive enough, that looking back on a troubleshooting or stressful event can they identify what they would have done differently? I want to make sure that the people I hire can manage stress, and think through an issue. I also try to ask them what they feel makes a good tech, and how they think their coworkers would describe them. It gets them thinking about the team, not just how they see themselves. I hope this helps! On Thu, Sep 12, 2019 at 2:06 PM Blanca Lopez via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I am going to interview people for Histotech position for the first > time...what are the best questions to ask? How do I prepare myself? what is > the I need to know that they are the best one? What should I ask or choose? > Is good to put them in action like cutting or staining to check on their > skills or what are your recommendations? thank you for your help > > Blanca Lopez HT (ASCP)cm > Senior Histotechnologist > UT Southwestern Medical Center > Harold C. Simmons Comprehensive Cancer Center > UTSTR Biorepository Tissue Lab > 6000 Harry Hines Blvd NB5.102 > Dallas, Texas 75390 > 214-648-7598 > blanca.lopez at utsouthwestern.edu > > > > ________________________________ > > UT Southwestern > > > Medical Center > > > > The future of medicine, today. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Erin McCarthy, HT (ASCP) Histology Supervisor Tempus Labs 600 W. Chicago Ave. Chicago IL 60654 Cell: (708)269-8610 -- This email and any attachments may contain privileged and confidential information and/or protected health information (PHI) that is protected by federal and state privacy laws.? It is intended solely for the use of Tempus Labs and the recipient(s) named above.? Nothing contained in this communication and any attachments thereto is intended to waive any privileges or rights of confidentiality.? If you are not the recipient, or the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any review, dissemination, distribution, printing or copying of this email message and/or any attachments is strictly prohibited.?* If you have received this transmission in error, please notify us immediately at?**(855)-442-8305**? and permanently delete this email and any attachments*. From amurvosh at advancederm.net Fri Sep 13 09:27:37 2019 From: amurvosh at advancederm.net (Anne Murvosh) Date: Fri, 13 Sep 2019 14:27:37 +0000 Subject: [Histonet] Help to interview new employees for the first time In-Reply-To: <973afa7d97ab413f9552b6fd7abe11a3@SWMS13MAIL10.swmed.org> References: <973afa7d97ab413f9552b6fd7abe11a3@SWMS13MAIL10.swmed.org> Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7C01FEE@Exchange.Advancederm.net> I'm not sure if this is an option, but we used to melt down old blocks and make the HT embed and cut them. This showed us how good and quick they were and if they actually new what they were doing. Anne -----Original Message----- From: Blanca Lopez via Histonet Sent: Thursday, September 12, 2019 11:48 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Help to interview new employees for the first time I am going to interview people for Histotech position for the first time...what are the best questions to ask? How do I prepare myself? what is the I need to know that they are the best one? What should I ask or choose? Is good to put them in action like cutting or staining to check on their skills or what are your recommendations? thank you for your help Blanca Lopez HT (ASCP)cm Senior Histotechnologist UT Southwestern Medical Center Harold C. Simmons Comprehensive Cancer Center UTSTR Biorepository Tissue Lab 6000 Harry Hines Blvd NB5.102 Dallas, Texas 75390 214-648-7598 blanca.lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Koepplin.Justin at mayo.edu Fri Sep 13 09:41:15 2019 From: Koepplin.Justin at mayo.edu (Koepplin, Justin W.) Date: Fri, 13 Sep 2019 14:41:15 +0000 Subject: [Histonet] Tissue processors for electron microscopy Message-ID: <771925$cd2c1q@ironport10.mayo.edu> Hello, I'm looking for recommendations or some insider information for tissue processors for electron microscopy specimens. We are currently using an older Leica EM TP that we are looking to replace/upgrade. As far as this one and the Lynx II, I haven't seen many options. I thought I'd try to get a feel for what others are using and what's working. Currently, we process around 30 specimens/day with a steadily increasing volume. I believe the mPREP was tried in the past and was not a good fit for the lab due to problems with the mPREP handling very small specimens. Any information would be greatly appreciated. Thank you, Justin Development Technologist Rochester, MN From foreightl at gmail.com Fri Sep 13 10:52:33 2019 From: foreightl at gmail.com (Patrick Laurie) Date: Fri, 13 Sep 2019 11:52:33 -0400 Subject: [Histonet] Help to interview new employees for the first time In-Reply-To: <22BDD9AABC13E24E95D1CF064B75C4B7C01FEE@Exchange.Advancederm.net> References: <973afa7d97ab413f9552b6fd7abe11a3@SWMS13MAIL10.swmed.org> <22BDD9AABC13E24E95D1CF064B75C4B7C01FEE@Exchange.Advancederm.net> Message-ID: I'm also a fan of having any fellow employees available ask questions. I have my staff limit it to histology related subjects, but my thought is if they are going to be the ones working directly with them, it helps to have their opinions. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Fri, Sep 13, 2019 at 10:40 AM Anne Murvosh via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I'm not sure if this is an option, but we used to melt down old blocks and > make the HT embed and cut them. This showed us how good and quick they were > and if they actually new what they were doing. Anne > > -----Original Message----- > From: Blanca Lopez via Histonet > Sent: Thursday, September 12, 2019 11:48 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Help to interview new employees for the first time > > I am going to interview people for Histotech position for the first > time...what are the best questions to ask? How do I prepare myself? what is > the I need to know that they are the best one? What should I ask or choose? > Is good to put them in action like cutting or staining to check on their > skills or what are your recommendations? thank you for your help > > Blanca Lopez HT (ASCP)cm > Senior Histotechnologist > UT Southwestern Medical Center > Harold C. Simmons Comprehensive Cancer Center UTSTR Biorepository Tissue > Lab > 6000 Harry Hines Blvd NB5.102 > Dallas, Texas 75390 > 214-648-7598 > blanca.lopez at utsouthwestern.edu > > > > ________________________________ > > UT Southwestern > > > Medical Center > > > > The future of medicine, today. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tbraud at holyredeemer.com Fri Sep 13 12:37:57 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 13 Sep 2019 17:37:57 +0000 Subject: [Histonet] Interview Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B5C9C6@HRHEX02-HOS.holyredeemer.local> Just a note: The liability of having a interviewee cut themselves operating an instrument that they have not been signed off as competent is HUGE. I think your company/labs legal team would say absolutely not. It would be nice to assess their cutting and other skills ahead of time, but that is what the probationary period is for. Don't be afraid to let an underperformer go during probation. More than likely, a good personality fit is the most important, anyway. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 5. Help to interview new employees for the first time (Blanca Lopez) From Christopher.Hagon at act.gov.au Sun Sep 15 22:43:00 2019 From: Christopher.Hagon at act.gov.au (Hagon, Christopher (Health)) Date: Mon, 16 Sep 2019 03:43:00 +0000 Subject: [Histonet] Permanent mountant for Oil Red O Message-ID: UNCLASSIFIED Hello Histonetters, We do Oil Red O stains on frozen section post mortem tissue, and need to find a permanent aqueous mounting medium. We used to use the Thermo-Fisher Perm-mount, but can't seem to get it anymore. As they are looking for fat deposits, we can't use any solvent based solutions, and the only aqueous ones we've found aren't permanent. We have to send the slides off site after staining, so the chances of the coverslip moving in transport is fairly high. Does anyone else have this issue and what did you end up using? Thanks in advance, Chris Hagon | Senior Scientist, Anatomical Pathology ACT Pathology | health.act.gov.au Phone (02) 5124 2874 ----------------------------------------------------------------------- This email, and any attachments, may be confidential and also privileged. If you are not the intended recipient, please notify the sender and delete all copies of this transmission along with any attachments immediately. You should not copy or use it for any purpose, nor disclose its contents to any other person. ----------------------------------------------------------------------- From Christopher.Hagon at act.gov.au Sun Sep 15 23:37:34 2019 From: Christopher.Hagon at act.gov.au (Hagon, Christopher (Health)) Date: Mon, 16 Sep 2019 04:37:34 +0000 Subject: [Histonet] Oil Red O permanent mountant Message-ID: UNCLASSIFIED Hi again, Meant to say ThermoFisher Aqua Mount, not Permount in my previous post. Apologies, Chris Hagon | Senior Scientist, Anatomical Pathology ACT Pathology | health.act.gov.au Phone (02) 5124 2874 ----------------------------------------------------------------------- This email, and any attachments, may be confidential and also privileged. If you are not the intended recipient, please notify the sender and delete all copies of this transmission along with any attachments immediately. You should not copy or use it for any purpose, nor disclose its contents to any other person. ----------------------------------------------------------------------- From Christopher.Sheeder at seattlechildrens.org Mon Sep 16 10:52:23 2019 From: Christopher.Sheeder at seattlechildrens.org (Sheeder, Christopher) Date: Mon, 16 Sep 2019 15:52:23 +0000 Subject: [Histonet] Help to interview new employees for the first time In-Reply-To: <973afa7d97ab413f9552b6fd7abe11a3@SWMS13MAIL10.swmed.org> References: <973afa7d97ab413f9552b6fd7abe11a3@SWMS13MAIL10.swmed.org> Message-ID: <951ea871dea148c4bb7f9e3b4d6369af@seattlechildrens.org> Hi Blanca, Hiring has become more difficult in recent years. Most employers now only verify past employment. They cannot divulge any corrective actions, performance issues or firings. References are hand-picked by the applicant so you don't get the whole picture there either. I typically ask them about their experiences in histology. Obstacles they have overcome, how to handle difficult customers (angry physicians) etc. If you can give the other techs a chance to ask the candidate questions, great, otherwise see what questions your techs want to know. I fully agree with Terri Braud's response. Do not have them perform any type of function. That is the purpose of the probationary period. The best interview question that was ever asked of me..."Name 12 uses for a pencil". Seems silly, but it's not about the answer, it's about demonstrating your creative problem solving skills. (it was tough, but I came up with 12!) Best of luck! Christopher Sheeder, HT(ASCP)QIHC Pathology Manager | Department of Laboratories Seattle Children's Hospital -----Original Message----- From: Blanca Lopez Sent: Thursday, September 12, 2019 11:48 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Help to interview new employees for the first time I am going to interview people for Histotech position for the first time...what are the best questions to ask? How do I prepare myself? what is the I need to know that they are the best one? What should I ask or choose? Is good to put them in action like cutting or staining to check on their skills or what are your recommendations? thank you for your help Blanca Lopez HT (ASCP)cm Senior Histotechnologist UT Southwestern Medical Center Harold C. Simmons Comprehensive Cancer Center UTSTR Biorepository Tissue Lab 6000 Harry Hines Blvd NB5.102 Dallas, Texas 75390 214-648-7598 blanca.lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jkiernan at uwo.ca Mon Sep 16 12:30:42 2019 From: jkiernan at uwo.ca (John Kiernan) Date: Mon, 16 Sep 2019 17:30:42 +0000 Subject: [Histonet] Permanent mountant for Oil Red O In-Reply-To: References: Message-ID: Fructose syrup gives a hard set, but it's a bit acidic (I don't know why) and is therefore incompatible with simple basic dyes, but it's OK for Oil red O and the Sudans. Fructose (also called laevulose or levulose) 15 g Distilled water 5 ml Leave at ~60C (in paraffin oven) for 2 or 3 days for all the fructose to dissolve. Don't let the water evaporate! The transparent syrup keeps well for 2-3 months at room temp. With long storage fructose crystallizes under the coverslip; this can be retarded by sealing the adges with a resinous mounting medium such as DPX. (You can buy fructose powder for cooking - about $3 a pound on the the internet. Cf $25-80 from chemical supply houses.) Another good one is polyvinylpyrrolidone: PVP (m.w. 10,000) 25g Water (or a phosphate buffer pH 7.4) 25ml When dissolved (several hours, magnetic stirring), add 1ml glycerol and a small crystal of thymol. Keeps for up to 3 years. Discard if it becomes cloudy. Use the buffered variety if the preparation has been stained or counterstained with a basic dye like toluidine blue or neutral red. This is less viscous than fructose syrup, and also has a lower refractive index, but with evaporation at the edges of the coverslip it gradually (weeks) becomes harder, and its refractive index increases almost to that of a resinous mountant. Probably you won't want to wait before shipping the slides elsewhere. PVP costs more than fructose (about $150 per pound for PVP10). This short article about aqueous mounting media is rather old (1997) but probably still OK: http://publish.uwo.ca/~jkiernan/aqmount.htm John Kiernan London, Canada = = = ________________________________ From: Hagon, Christopher (Health) via Histonet Sent: 15 September 2019 22:43 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Permanent mountant for Oil Red O UNCLASSIFIED Hello Histonetters, We do Oil Red O stains on frozen section post mortem tissue, and need to find a permanent aqueous mounting medium. We used to use the Thermo-Fisher Perm-mount, but can't seem to get it anymore. As they are looking for fat deposits, we can't use any solvent based solutions, and the only aqueous ones we've found aren't permanent. We have to send the slides off site after staining, so the chances of the coverslip moving in transport is fairly high. Does anyone else have this issue and what did you end up using? Thanks in advance, Chris Hagon | Senior Scientist, Anatomical Pathology ACT Pathology | health.act.gov.au Phone (02) 5124 2874 ----------------------------------------------------------------------- This email, and any attachments, may be confidential and also privileged. If you are not the intended recipient, please notify the sender and delete all copies of this transmission along with any attachments immediately. You should not copy or use it for any purpose, nor disclose its contents to any other person. ----------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vavalos at dermatologyaz.com Mon Sep 16 15:02:57 2019 From: vavalos at dermatologyaz.com (Vanessa Avalos) Date: Mon, 16 Sep 2019 20:02:57 +0000 Subject: [Histonet] New lab set up Message-ID: We will be setting up our lab on the 2nd floor in the next few months and since it's been a while since I have set up a new lab I am looking for any ideas you all have. Our lab is a small one that does derm/H&E only for our 3 locations. I have always stood to section, but am thinking of getting a higher chair and sitting. What do you all think of sitting vs standing, along with counter height suggestions? All input whether it pertains to our lab or not will be appreciated because I am sure there will be something I will forget. Thanks! V. Avalos AD HISTO From llewllew at shaw.ca Mon Sep 16 19:12:50 2019 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Mon, 16 Sep 2019 17:12:50 -0700 Subject: [Histonet] New lab set up In-Reply-To: References: Message-ID: I have sectioned both standing and sitting, and much prefer sitting. However, due to back problems I found it necessary to have a stand built for the microtome so that when I was sctioning I reached slightly upwards rather than crouched over. This all but eliminated the strain on my back. The stand was basically a five sided box with an open front made from heavy plywood and sized to fit my requirements. The front was open and very useful for slides, pens, tissues and so on. I also used a heavy draughting chair which was higher than a normal chair. The combination worked very well for daily sectioning over a 20 year period. Bryan Llewellyn Vanessa Avalos via Histonet wrote: > We will be setting up our lab on the 2nd floor in the next few months and since it's been a while since I have set up a new lab I am looking for any ideas you all have. Our lab is a small one that does derm/H&E only for our 3 locations. > I have always stood to section, but am thinking of getting a higher chair and sitting. What do you all think of sitting vs standing, along with counter height suggestions? All input whether it pertains to our lab or not will be appreciated because I am sure there will be something I will forget. > > Thanks! > V. Avalos > AD HISTO > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Blanca.Lopez at UTSouthwestern.edu Tue Sep 17 11:46:38 2019 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Tue, 17 Sep 2019 16:46:38 +0000 Subject: [Histonet] instruments needed Message-ID: It's time to updated the lab so I'm looking for those items but they have to be new please! If you are or know vendors please sent me an email. What are your recommendations? Any combos that you can make for me please? Quotes or prices asap please thanks Water bath Embedding center Processor Microtome Racks and containers used or not for a autostainer XL Leica thanks Blanca Lopez HT (ASCP)cm Senior Histotechnologist UT Southwestern Medical Center Harold C. Simmons Comprehensive Cancer Center UTSTR Biorepository Tissue Lab 6000 Harry Hines Blvd NB5.102 Dallas, Texas 75390 214-648-7598 blanca.lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From bertjekb at gmail.com Tue Sep 17 23:12:35 2019 From: bertjekb at gmail.com (B kB) Date: Wed, 18 Sep 2019 06:12:35 +0200 Subject: [Histonet] Permanent mountant for Oil Red O In-Reply-To: References: Message-ID: Hi Christopher, You van try Imsol-mount Vendors are for example Sigmaaldrich or VWR . After a staining, airdry the solide. Cover with mislopen-mount and let this dry for a while ( no problem with higher temps). After that, You can cover it with glass or tape. Goed luck, Bert klein brink Gelre Hospital, Apeldoorn, Netherlands Op ma 16 sep. 2019 om 06:04 schreef Hagon, Christopher (Health) via Histonet > UNCLASSIFIED > > Hello Histonetters, > > We do Oil Red O stains on frozen section post mortem tissue, and need to > find a permanent aqueous mounting medium. We used to use the Thermo-Fisher > Perm-mount, but can't seem to get it anymore. As they are looking for fat > deposits, we can't use any solvent based solutions, and the only aqueous > ones we've found aren't permanent. We have to send the slides off site > after staining, so the chances of the coverslip moving in transport is > fairly high. > > Does anyone else have this issue and what did you end up using? > > Thanks in advance, > > Chris Hagon | Senior Scientist, Anatomical Pathology > ACT Pathology | health.act.gov.au > Phone (02) 5124 2874 > > > > > > ----------------------------------------------------------------------- > This email, and any attachments, may be confidential and also privileged. > If you are not the intended recipient, please notify the sender and delete > all copies of this transmission along with any attachments immediately. You > should not copy or use it for any purpose, nor disclose its contents to any > other person. > ----------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Erin.Martin at ucsf.edu Wed Sep 18 10:03:38 2019 From: Erin.Martin at ucsf.edu (Martin, Erin) Date: Wed, 18 Sep 2019 15:03:38 +0000 Subject: [Histonet] New lab set up In-Reply-To: References: Message-ID: Hi Vanessa! It's funny that you asked this question because we were just talking about this a few days ago in my lab. Our tables are low and we all sit. We were discussing that we would like to have higher tables so that we could have the option to stand or sit on a higher chair. Since we are usually embedding for 4 to 5 hours the ability to change positions would be nice. So my suggestion would be a get a higher table so that you could sit or stand depending on your mood! Have a great Wednesday! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero Street, RM 230 San Francisco, CA 94115 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. ________________________________ From: Vanessa Avalos Sent: Monday, September 16, 2019 1:02 PM To: HISTONET LISTS Subject: [Histonet] New lab set up We will be setting up our lab on the 2nd floor in the next few months and since it's been a while since I have set up a new lab I am looking for any ideas you all have. Our lab is a small one that does derm/H&E only for our 3 locations. I have always stood to section, but am thinking of getting a higher chair and sitting. What do you all think of sitting vs standing, along with counter height suggestions? All input whether it pertains to our lab or not will be appreciated because I am sure there will be something I will forget. Thanks! V. Avalos AD HISTO From cfields at mlkch.org Wed Sep 18 10:51:22 2019 From: cfields at mlkch.org (Carol G Fields) Date: Wed, 18 Sep 2019 15:51:22 +0000 Subject: [Histonet] Policies to be posted in Morgue Message-ID: Hi Fellow Histo Techs, What policies need to be posted in the Morgue for a CAP Inspection. Mine were removed and CAP window is open. Thank you in advance, Carole Carole Fields, HT (ASCP) Lead Histotechnologist, Pathology Laboratory Martin Luther King Jr. Community Hospital 1680 E. 120th Street Los Angeles, CA 90059 Fax# 424-296-3932 cfields at mlkch.org This email message and any files transmitted are sent with confidentiality in mind and contain privileged or copyright information. You must not present this message to another party without gaining permission from the sender. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. Any views expressed in this message are those of the sender, except where the sender specifically states them to be the views of Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. If you have received this message in error, please notify the sender immediately, and delete this email from your system. We do not guarantee that this material is free from viruses or any other defects although due care has been taken to minimize the risk. From Valerie.Hannen at parrishmed.com Thu Sep 19 05:42:14 2019 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Thu, 19 Sep 2019 10:42:14 +0000 Subject: [Histonet] Steel blade sharpening Message-ID: <0d7969cc054b4eb6a9324141f32bc3af@PMSVRLEX02.parrishmed.local> Good Morning all, We am coming out of the dark ages!! We are going to be transitioning to disposable blades in the very near future. My main problem now , is that I may not get the equipment( disposable blade holder or new Microtomes) that I will need soon enough ( we are running out of compounds needed to sharpen these blades ourselves). I am waiting for review and approval of this equipment, so in the mean time I may need to send my steel microtome blades out to be sharpened. We are on the East Coast of Florida ( @ 1 hour away from Orlando). I am reaching out to you all asking if you know of any companies relatively close to me that sharpen these blades. Thanks so much in advance for any help and insight you may give me. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com From amurvosh at advancederm.net Thu Sep 19 11:42:36 2019 From: amurvosh at advancederm.net (Anne Murvosh) Date: Thu, 19 Sep 2019 16:42:36 +0000 Subject: [Histonet] Yearly review Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7C02323@Exchange.Advancederm.net> Hey everyone, does the yearly employee competency assessments need to be kept for just 2 years or for the entire time an employee is there. I have some old ones I want to toss, but I just wanted to be sure. Thanks Anne From tbraud at holyredeemer.com Thu Sep 19 13:10:37 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 19 Sep 2019 18:10:37 +0000 Subject: [Histonet] Yearly Reviews Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B5F6D0@HRHEX02-HOS.holyredeemer.local> Hi Anne - Interesting question. Our Human Resources department keeps records of annual reviews, which include annual competencies, for 10 years following the separation of the employee from the system. I'm curious what others are doing, and does it vary from state to state. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Message: 2 Date: Thu, 19 Sep 2019 16:42:36 +0000 From: Anne Murvosh To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Yearly review Hey everyone, does the yearly employee competency assessments need to be kept for just 2 years or for the entire time an employee is there. I have some old ones I want to toss, but I just wanted to be sure. Thanks Anne ------------------------------ From boneimage8 at gmail.com Thu Sep 19 13:34:08 2019 From: boneimage8 at gmail.com (Marc DeCarlo) Date: Thu, 19 Sep 2019 13:34:08 -0500 Subject: [Histonet] Steel blade sharpening In-Reply-To: <0d7969cc054b4eb6a9324141f32bc3af@PMSVRLEX02.parrishmed.local> References: <0d7969cc054b4eb6a9324141f32bc3af@PMSVRLEX02.parrishmed.local> Message-ID: Dorn & Hart sharpens microtome knives & is on the Florida / Alabama border. I?ve used them for year. Marc D On Thu, Sep 19, 2019 at 5:53 AM Hannen, Valerie via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good Morning all, > > We am coming out of the dark ages!! We are going to be transitioning to > disposable blades in the very near future. My main problem now , is that I > may not get the equipment( disposable blade holder or new Microtomes) that > I > > will need soon enough ( we are running out of compounds needed to sharpen > these blades ourselves). > > I am waiting for review and approval of this equipment, so in the mean > time I may need to send my steel microtome blades out to be sharpened. We > are on the East Coast of Florida ( @ 1 hour away from Orlando). > > I am reaching out to you all asking if you know of any companies > relatively close to me that sharpen these blades. > > Thanks so much in advance for any help and insight you may give me. > > > Valerie Hannen,MLT(ASCP),HTL,SU (FL) > Section Chief, Histology > Parrish Medical Center > 951 N. Washington Ave. > Titusville,Florida 32796 > T: (321)268-6333 ext. 7506 > F: (321) 268-6149 > valerie.hannen at parrishmed.com > www.parrishmed.com > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From john.garratt at ciqc.ca Thu Sep 19 15:13:24 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 19 Sep 2019 20:13:24 +0000 Subject: [Histonet] Steel blade sharpening In-Reply-To: <0d7969cc054b4eb6a9324141f32bc3af@PMSVRLEX02.parrishmed.local> References: <0d7969cc054b4eb6a9324141f32bc3af@PMSVRLEX02.parrishmed.local> Message-ID: Way back 30 years ago when we moved to disposable blades I negotiated with the blade supplier to provide a blade holders for our microtomes for free. These blade holders replace the steel knife so you do not need to buy a new part for your microtome. If I remember right these blade holders were sold by Feather. Good luck John www.ciqc.ca ??????? Original Message ??????? On Thursday, September 19, 2019 3:42 AM, Hannen, Valerie via Histonet wrote: > Good Morning all, > > We am coming out of the dark ages!! We are going to be transitioning to disposable blades in the very near future. My main problem now , is that I may not get the equipment( disposable blade holder or new Microtomes) that I > > will need soon enough ( we are running out of compounds needed to sharpen these blades ourselves). > > I am waiting for review and approval of this equipment, so in the mean time I may need to send my steel microtome blades out to be sharpened. We are on the East Coast of Florida ( @ 1 hour away from Orlando). > > I am reaching out to you all asking if you know of any companies relatively close to me that sharpen these blades. > > Thanks so much in advance for any help and insight you may give me. > > Valerie Hannen,MLT(ASCP),HTL,SU (FL) > Section Chief, Histology > Parrish Medical Center > 951 N. Washington Ave. > Titusville,Florida 32796 > T: (321)268-6333 ext. 7506 > F: (321) 268-6149 > valerie.hannen at parrishmed.commailto:valerie.hannen at parrishmed.com > www.parrishmed.com > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aperl at caremount.com Thu Sep 19 15:26:03 2019 From: aperl at caremount.com (Perl, Alison) Date: Thu, 19 Sep 2019 20:26:03 +0000 Subject: [Histonet] Yearly Reviews In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F801C1B5F6D0@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F801C1B5F6D0@HRHEX02-HOS.holyredeemer.local> Message-ID: <9a059f692ddc45b4b7cf22586db4d4a9@MK-EXMB02.mkmg.com> We keep them (per NYS regulation) for the term of the persons employment, plus 2 years -----Original Message----- From: Terri Braud via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 19, 2019 2:11 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [EXTERNAL] Re: [Histonet] Yearly Reviews Hi Anne - Interesting question. Our Human Resources department keeps records of annual reviews, which include annual competencies, for 10 years following the separation of the employee from the system. I'm curious what others are doing, and does it vary from state to state. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Message: 2 Date: Thu, 19 Sep 2019 16:42:36 +0000 From: Anne Murvosh To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Yearly review Hey everyone, does the yearly employee competency assessments need to be kept for just 2 years or for the entire time an employee is there. I have some old ones I want to toss, but I just wanted to be sure. Thanks Anne ------------------------------ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DKnutson at primecare.org Wed Sep 25 07:50:44 2019 From: DKnutson at primecare.org (Knutson, Deanne) Date: Wed, 25 Sep 2019 07:50:44 -0500 Subject: [Histonet] Flammable Sprays in Cryostats Message-ID: <1E0E2B14C709174B8AC2BE0AE7F768330159B575665C@EXCHANGE2K7.staprimecare.org> I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable freezing sprays in their cryostats. What are others using in their cryostats to instantly freeze specimens? Thank you. Deanne Knutson Supervisor Anatomic Pathology dknutson at primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From akemiat3377 at gmail.com Wed Sep 25 09:05:20 2019 From: akemiat3377 at gmail.com (Akemi) Date: Wed, 25 Sep 2019 09:05:20 -0500 Subject: [Histonet] Flammable Sprays in Cryostats In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F768330159B575665C@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F768330159B575665C@EXCHANGE2K7.staprimecare.org> Message-ID: <19DB25EB-9944-406B-917C-0878D6F15CA0@gmail.com> We are purchasing two of their newer UV cryostats and they were adamant that we use a non flammable freezing spray because it can damage the UV unit and have potential for exploding. Akemi Allison Sent from my iPhone > On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet wrote: > > I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable > freezing sprays in their cryostats. What are others using in their cryostats to instantly freeze specimens? > > Thank you. > > > Deanne Knutson > Supervisor > Anatomic Pathology > dknutson at primecare.org > > > > > > > ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen at parrishmed.com Wed Sep 25 10:49:00 2019 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Wed, 25 Sep 2019 15:49:00 +0000 Subject: [Histonet] Flammable Sprays in Cryostats In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F768330159B575665C@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F768330159B575665C@EXCHANGE2K7.staprimecare.org> Message-ID: <68e18a8b516a49d4b033de54e175687d@PMSVRLEX02.parrishmed.local> We received the same letter months ago. We changed to a non-flammable spray. It is called STATFREEZE, we purchase it from STATLAB. The catalog number is SL202/134a. Be careful if you order it because they also sell a product called STATFREEZE that is flammable. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Knutson, Deanne via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, September 25, 2019 8:51 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [EXTERNAL Sender] [Histonet] Flammable Sprays in Cryostats This message came from an external source. Please do not click links or open attachments if unexpected or unusual. Begin Original Message: ---------------------------------------------------------------------- I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable freezing sprays in their cryostats. What are others using in their cryostats to instantly freeze specimens? Thank you. Deanne Knutson Supervisor Anatomic Pathology dknutson at primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=Xk0H5Lt1SX4yLDi3Z36FWCjxWMexCFmv9kQz5nprqM4&r=S0NRG57vjoVygxIXrxrniUhxWkoaXBwDrgVZ_u7KUmY&m=rqHf4UNcHbvp592ujkRAO5WLlEJw9KUFgBy00GsLhqs&s=aDgPVL1SLa_CnToePuo4VME9QjJOweGzyLEoVKJSWPg&e= From CIngles at uwhealth.org Wed Sep 25 11:42:30 2019 From: CIngles at uwhealth.org (Ingles Claire) Date: Wed, 25 Sep 2019 16:42:30 +0000 Subject: [Histonet] Flammable Sprays in Cryostats In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F768330159B575665C@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F768330159B575665C@EXCHANGE2K7.staprimecare.org> Message-ID: Liquid Nitrogen guns. Claire ________________________________ From: Knutson, Deanne via Histonet Sent: Wednesday, September 25, 2019 7:50 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Flammable Sprays in Cryostats WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable freezing sprays in their cryostats. What are others using in their cryostats to instantly freeze specimens? Thank you. Deanne Knutson Supervisor Anatomic Pathology dknutson at primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From naira.margaryan at hsc.wvu.edu Wed Sep 25 12:03:31 2019 From: naira.margaryan at hsc.wvu.edu (Margaryan, Naira) Date: Wed, 25 Sep 2019 17:03:31 +0000 Subject: [Histonet] job search in Chicago area Message-ID: Hello histonetters, Please share this email with your colleagues. I am in a job searching process in Chicago area to be close to my family as my appointment in West Virginia University Research Corporation, Morgantown, WV is coming to an end since my long-standing supervisor is closing her research laboratory. I have had the privilege of establishing a Research Histology Core in Stanley Manne Children's Research Institute, Chicago, IL as well as a Research Histology Service in West Virginia University Research Corporation, Morgantown, WV, where I have provided histology services to scientists in various disciplines to support their research projects. I have also trained students enrolled in health science programs in the areas of routine histology and animal work. My experience in cancer research with background in histology and in veterinary medicine has given me a unique opportunity to translate my knowledge from basic science to clinically relevant applications for diagnostic, prognostic and therapeutic development as they relate to cancer. My contributions to various research projects have included in vitro and in vivo work of predictive cancer biomarkers. I have been involved in testing novel humanized targeted antibody-based therapy in the treatment of human cancer in xenograft mouse models. I am confident that my skills and experience would allow me to be a great fit for the research laboratory where I could provide excellent support for a wide range of scientific projects. I am a fast learner, a hard worker and a strong team player. Furthermore, I am able to transfer a DAKO autostainer and an H&E station as part of my relocation. My Resume and Curriculum Vitae are available by request and provide the more comprehensive list of my qualifications and accomplishments. I am very interested in meeting to discuss how I can best contribute to the work in research laboratory. Sincerely, Naira Margaryan Naira V. Margaryan, D.V.M., Ph.D. Senior Research Scientist West Virginia University Robert C. Byrd Health Sciences Center Department of Biochemistry PO Box 9142 200 Erma Byrd Biomedical Research Bldg. 108 Biomedical Road Morgantown, WV 26506 Tel: 304-293-2213 naira.margaryan at hsc.wvu.edu From tbraud at holyredeemer.com Wed Sep 25 12:31:15 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 25 Sep 2019 17:31:15 +0000 Subject: [Histonet] Freezing spray in cryostats Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B60286@HRHEX02-HOS.holyredeemer.local> My question is why would you ever want to use an aerosol in the cryostat, taking a chance on aerosolizing some nasty bug? Is the 30 seconds you save in using freezing spray worth the exposure to TB or goodness-knows-what? We use a simple metal heat extractor (comes standard in our Leicas) and place it on top of the freezing block to accelerate the process. We never have issues with turn around times. For me, no freezing spray. Ever. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Wednesday, September 25, 2019 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 190, Issue 18 CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links or opening attachments. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Flammable Sprays in Cryostats (Knutson, Deanne) 2. Re: Flammable Sprays in Cryostats (Akemi) 3. Re: Flammable Sprays in Cryostats (Hannen, Valerie) 4. Re: Flammable Sprays in Cryostats (Ingles Claire) ---------------------------------------------------------------------- Message: 1 Date: Wed, 25 Sep 2019 07:50:44 -0500 From: "Knutson, Deanne" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Flammable Sprays in Cryostats Message-ID: <1E0E2B14C709174B8AC2BE0AE7F768330159B575665C at EXCHANGE2K7.staprimecare.org> Content-Type: text/plain; charset="us-ascii" I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable freezing sprays in their cryostats. What are others using in their cryostats to instantly freeze specimens? Thank you. Deanne Knutson Supervisor Anatomic Pathology dknutson at primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ------------------------------ Message: 2 Date: Wed, 25 Sep 2019 09:05:20 -0500 From: Akemi To: "Knutson, Deanne" , Histonet Subject: Re: [Histonet] Flammable Sprays in Cryostats Message-ID: <19DB25EB-9944-406B-917C-0878D6F15CA0 at gmail.com> Content-Type: text/plain; charset=us-ascii We are purchasing two of their newer UV cryostats and they were adamant that we use a non flammable freezing spray because it can damage the UV unit and have potential for exploding. Akemi Allison Sent from my iPhone > On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet wrote: > > I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable > freezing sprays in their cryostats. What are others using in their cryostats to instantly freeze specimens? > > Thank you. > > > Deanne Knutson > Supervisor > Anatomic Pathology > dknutson at primecare.org > > > > > > > ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Wed, 25 Sep 2019 15:49:00 +0000 From: "Hannen, Valerie" To: "'Knutson, Deanne'" Cc: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Flammable Sprays in Cryostats Message-ID: <68e18a8b516a49d4b033de54e175687d at PMSVRLEX02.parrishmed.local> Content-Type: text/plain; charset="us-ascii" We received the same letter months ago. We changed to a non-flammable spray. It is called STATFREEZE, we purchase it from STATLAB. The catalog number is SL202/134a. Be careful if you order it because they also sell a product called STATFREEZE that is flammable. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Knutson, Deanne via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, September 25, 2019 8:51 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [EXTERNAL Sender] [Histonet] Flammable Sprays in Cryostats This message came from an external source. Please do not click links or open attachments if unexpected or unusual. Begin Original Message: ---------------------------------------------------------------------- I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable freezing sprays in their cryostats. What are others using in their cryostats to instantly freeze specimens? Thank you. Deanne Knutson Supervisor Anatomic Pathology dknutson at primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=Xk0H5Lt1SX4yLDi3Z36FWCjxWMexCFmv9kQz5nprqM4&r=S0NRG57vjoVygxIXrxrniUhxWkoaXBwDrgVZ_u7KUmY&m=rqHf4UNcHbvp592ujkRAO5WLlEJw9KUFgBy00GsLhqs&s=aDgPVL1SLa_CnToePuo4VME9QjJOweGzyLEoVKJSWPg&e= ------------------------------ Message: 4 Date: Wed, 25 Sep 2019 16:42:30 +0000 From: Ingles Claire To: "'histonet at lists.utsouthwestern.edu'" , "Knutson, Deanne" Subject: Re: [Histonet] Flammable Sprays in Cryostats Message-ID: Content-Type: text/plain; charset="us-ascii" Liquid Nitrogen guns. Claire ________________________________ From: Knutson, Deanne via Histonet Sent: Wednesday, September 25, 2019 7:50 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Flammable Sprays in Cryostats WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable freezing sprays in their cryostats. What are others using in their cryostats to instantly freeze specimens? Thank you. Deanne Knutson Supervisor Anatomic Pathology dknutson at primecare.org ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 190, Issue 18 ***************************************** From criley at dpspa.com Wed Sep 25 13:00:53 2019 From: criley at dpspa.com (Charles Riley) Date: Wed, 25 Sep 2019 14:00:53 -0400 Subject: [Histonet] Freezing spray in cryostats In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F801C1B60286@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F801C1B60286@HRHEX02-HOS.holyredeemer.local> Message-ID: I have to agree with Terri. I also believe that CAP is going to be putting in regulations forbidding the use of freeze sprays in cryostats soon (at least I think I heard a rumor about this). Anyway another useful item is liquid nitrogen if you really need a quick freeze. Dip a brush into the liquid and lightly apply to the specimen. Not always recommended as could cause artifacts. On Wed, Sep 25, 2019 at 1:37 PM Terri Braud via Histonet < histonet at lists.utsouthwestern.edu> wrote: > My question is why would you ever want to use an aerosol in the cryostat, > taking a chance on aerosolizing some nasty bug? > Is the 30 seconds you save in using freezing spray worth the exposure to > TB or goodness-knows-what? > We use a simple metal heat extractor (comes standard in our Leicas) and > place it on top of the freezing block to accelerate the process. We never > have issues with turn around times. > For me, no freezing spray. Ever. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > -----Original Message----- > From: histonet-request at lists.utsouthwestern.edu [mailto: > histonet-request at lists.utsouthwestern.edu] > Sent: Wednesday, September 25, 2019 1:00 PM > To: histonet at lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 190, Issue 18 > > CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links > or opening attachments. > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Flammable Sprays in Cryostats (Knutson, Deanne) > 2. Re: Flammable Sprays in Cryostats (Akemi) > 3. Re: Flammable Sprays in Cryostats (Hannen, Valerie) > 4. Re: Flammable Sprays in Cryostats (Ingles Claire) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 25 Sep 2019 07:50:44 -0500 > From: "Knutson, Deanne" > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: [Histonet] Flammable Sprays in Cryostats > Message-ID: > < > 1E0E2B14C709174B8AC2BE0AE7F768330159B575665C at EXCHANGE2K7.staprimecare.org> > > Content-Type: text/plain; charset="us-ascii" > > I just received a letter from Leica Biosystems where they are prohibiting > the usage of flammable > freezing sprays in their cryostats. What are others using in their > cryostats to instantly freeze specimens? > > Thank you. > > > Deanne Knutson > Supervisor > Anatomic Pathology > dknutson at primecare.org > > > > > > > ________________________________ > This email may include confidential and privileged information. If this is > not intended for your use, please destroy immediately and contact the > sender of the message. > > This email and attachments contain information that may be confidential or > privileged. If you are not the intended recipient, notify the sender at > once and delete this message completely from your information system. > Further use, disclosure, or copying of information contained in this email > is not authorized, and any such action should not be construed as a waiver > of privilege or other confidentiality protections. > > > ------------------------------ > > Message: 2 > Date: Wed, 25 Sep 2019 09:05:20 -0500 > From: Akemi > To: "Knutson, Deanne" , Histonet > > Subject: Re: [Histonet] Flammable Sprays in Cryostats > Message-ID: <19DB25EB-9944-406B-917C-0878D6F15CA0 at gmail.com> > Content-Type: text/plain; charset=us-ascii > > We are purchasing two of their newer UV cryostats and they were adamant > that we use a non flammable freezing spray because it can damage the UV > unit and have potential for exploding. > > Akemi Allison > > Sent from my iPhone > > > On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > > I just received a letter from Leica Biosystems where they are > prohibiting the usage of flammable > > freezing sprays in their cryostats. What are others using in their > cryostats to instantly freeze specimens? > > > > Thank you. > > > > > > Deanne Knutson > > Supervisor > > Anatomic Pathology > > dknutson at primecare.org > > > > > > > > > > > > > > ________________________________ > > This email may include confidential and privileged information. If this > is not intended for your use, please destroy immediately and contact the > sender of the message. > > > > This email and attachments contain information that may be confidential > or privileged. If you are not the intended recipient, notify the sender at > once and delete this message completely from your information system. > Further use, disclosure, or copying of information contained in this email > is not authorized, and any such action should not be construed as a waiver > of privilege or other confidentiality protections. > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Wed, 25 Sep 2019 15:49:00 +0000 > From: "Hannen, Valerie" > To: "'Knutson, Deanne'" > Cc: "Histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Flammable Sprays in Cryostats > Message-ID: > <68e18a8b516a49d4b033de54e175687d at PMSVRLEX02.parrishmed.local> > Content-Type: text/plain; charset="us-ascii" > > We received the same letter months ago. We changed to a non-flammable > spray. It is called STATFREEZE, we purchase it from STATLAB. The catalog > number is SL202/134a. Be careful if you order it because they also sell a > product called STATFREEZE that is flammable. > > > > Valerie Hannen,MLT(ASCP),HTL,SU (FL) > Section Chief, Histology > Parrish Medical Center > 951 N. Washington Ave. > Titusville,Florida 32796 > T: (321)268-6333 ext. 7506 > F: (321) 268-6149 > valerie.hannen at parrishmed.com > www.parrishmed.com > > > > -----Original Message----- > From: Knutson, Deanne via Histonet [mailto: > histonet at lists.utsouthwestern.edu] > Sent: Wednesday, September 25, 2019 8:51 AM > To: 'histonet at lists.utsouthwestern.edu' > Subject: [EXTERNAL Sender] [Histonet] Flammable Sprays in Cryostats > > > This message came from an external source. Please do not click links or > open attachments if unexpected or unusual. > > Begin Original Message: > > ---------------------------------------------------------------------- > I just received a letter from Leica Biosystems where they are prohibiting > the usage of flammable > > freezing sprays in their cryostats. What are others using in their > cryostats to instantly freeze specimens? > > > > Thank you. > > > > > > Deanne Knutson > > Supervisor > > Anatomic Pathology > > dknutson at primecare.org > > > > > > > > > > > > > > ________________________________ > > This email may include confidential and privileged information. If this is > not intended for your use, please destroy immediately and contact the > sender of the message. > > > > This email and attachments contain information that may be confidential or > privileged. If you are not the intended recipient, notify the sender at > once and delete this message completely from your information system. > Further use, disclosure, or copying of information contained in this email > is not authorized, and any such action should not be construed as a waiver > of privilege or other confidentiality protections. > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=Xk0H5Lt1SX4yLDi3Z36FWCjxWMexCFmv9kQz5nprqM4&r=S0NRG57vjoVygxIXrxrniUhxWkoaXBwDrgVZ_u7KUmY&m=rqHf4UNcHbvp592ujkRAO5WLlEJw9KUFgBy00GsLhqs&s=aDgPVL1SLa_CnToePuo4VME9QjJOweGzyLEoVKJSWPg&e= > > > > > ------------------------------ > > Message: 4 > Date: Wed, 25 Sep 2019 16:42:30 +0000 > From: Ingles Claire > To: "'histonet at lists.utsouthwestern.edu'" > , "Knutson, Deanne" > > Subject: Re: [Histonet] Flammable Sprays in Cryostats > Message-ID: > < > DM5PR1701MB191313D76EF3A406C8C73F82C9870 at DM5PR1701MB1913.namprd17.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > Liquid Nitrogen guns. > Claire > ________________________________ > From: Knutson, Deanne via Histonet > Sent: Wednesday, September 25, 2019 7:50 AM > To: 'histonet at lists.utsouthwestern.edu' > > Subject: [Histonet] Flammable Sprays in Cryostats > > WARNING: This email appears to have originated outside of the UW Health > email system. > DO NOT CLICK on links or attachments unless you recognize the sender and > know the content is safe. > > > > > I just received a letter from Leica Biosystems where they are prohibiting > the usage of flammable > freezing sprays in their cryostats. What are others using in their > cryostats to instantly freeze specimens? > > Thank you. > > > Deanne Knutson > Supervisor > Anatomic Pathology > dknutson at primecare.org > > > > > > > ________________________________ > This email may include confidential and privileged information. If this is > not intended for your use, please destroy immediately and contact the > sender of the message. > > This email and attachments contain information that may be confidential or > privileged. If you are not the intended recipient, notify the sender at > once and delete this message completely from your information system. > Further use, disclosure, or copying of information contained in this email > is not authorized, and any such action should not be construed as a waiver > of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 190, Issue 18 > ***************************************** > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From akemiat3377 at gmail.com Wed Sep 25 13:25:35 2019 From: akemiat3377 at gmail.com (Akemi) Date: Wed, 25 Sep 2019 13:25:35 -0500 Subject: [Histonet] Freezing spray in cryostats In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F801C1B60286@HRHEX02-HOS.holyredeemer.local> Message-ID: I make my tech?s wear PPE?s, goggles/masks, gloves and N95 masks for potential TB and regular masks for normal tissue. Akemi Allison Sent from my iPhone > On Sep 25, 2019, at 1:00 PM, Charles Riley via Histonet wrote: > > I have to agree with Terri. I also believe that CAP is going to be putting > in regulations forbidding the use of freeze sprays in cryostats soon (at > least I think I heard a rumor about this). > > Anyway another useful item is liquid nitrogen if you really need a quick > freeze. Dip a brush into the liquid and lightly apply to the specimen. Not > always recommended as could cause artifacts. > > On Wed, Sep 25, 2019 at 1:37 PM Terri Braud via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> My question is why would you ever want to use an aerosol in the cryostat, >> taking a chance on aerosolizing some nasty bug? >> Is the 30 seconds you save in using freezing spray worth the exposure to >> TB or goodness-knows-what? >> We use a simple metal heat extractor (comes standard in our Leicas) and >> place it on top of the freezing block to accelerate the process. We never >> have issues with turn around times. >> For me, no freezing spray. Ever. >> >> Terri L. Braud, HT(ASCP) >> Anatomic Pathology Supervisor >> Laboratory >> Holy Redeemer Hospital >> 1648 Huntingdon Pike >> Meadowbrook, PA 19046 >> ph: 215-938-3689 >> fax: 215-938-3874 >> Care, Comfort, and Heal >> >> -----Original Message----- >> From: histonet-request at lists.utsouthwestern.edu [mailto: >> histonet-request at lists.utsouthwestern.edu] >> Sent: Wednesday, September 25, 2019 1:00 PM >> To: histonet at lists.utsouthwestern.edu >> Subject: Histonet Digest, Vol 190, Issue 18 >> >> CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links >> or opening attachments. >> >> Send Histonet mailing list submissions to >> histonet at lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-request at lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-owner at lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. Flammable Sprays in Cryostats (Knutson, Deanne) >> 2. Re: Flammable Sprays in Cryostats (Akemi) >> 3. Re: Flammable Sprays in Cryostats (Hannen, Valerie) >> 4. Re: Flammable Sprays in Cryostats (Ingles Claire) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Wed, 25 Sep 2019 07:50:44 -0500 >> From: "Knutson, Deanne" >> To: "'histonet at lists.utsouthwestern.edu'" >> >> Subject: [Histonet] Flammable Sprays in Cryostats >> Message-ID: >> < >> 1E0E2B14C709174B8AC2BE0AE7F768330159B575665C at EXCHANGE2K7.staprimecare.org> >> >> Content-Type: text/plain; charset="us-ascii" >> >> I just received a letter from Leica Biosystems where they are prohibiting >> the usage of flammable >> freezing sprays in their cryostats. What are others using in their >> cryostats to instantly freeze specimens? >> >> Thank you. >> >> >> Deanne Knutson >> Supervisor >> Anatomic Pathology >> dknutson at primecare.org >> >> >> >> >> >> >> ________________________________ >> This email may include confidential and privileged information. If this is >> not intended for your use, please destroy immediately and contact the >> sender of the message. >> >> This email and attachments contain information that may be confidential or >> privileged. If you are not the intended recipient, notify the sender at >> once and delete this message completely from your information system. >> Further use, disclosure, or copying of information contained in this email >> is not authorized, and any such action should not be construed as a waiver >> of privilege or other confidentiality protections. >> >> >> ------------------------------ >> >> Message: 2 >> Date: Wed, 25 Sep 2019 09:05:20 -0500 >> From: Akemi >> To: "Knutson, Deanne" , Histonet >> >> Subject: Re: [Histonet] Flammable Sprays in Cryostats >> Message-ID: <19DB25EB-9944-406B-917C-0878D6F15CA0 at gmail.com> >> Content-Type: text/plain; charset=us-ascii >> >> We are purchasing two of their newer UV cryostats and they were adamant >> that we use a non flammable freezing spray because it can damage the UV >> unit and have potential for exploding. >> >> Akemi Allison >> >> Sent from my iPhone >> >>> On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet < >> histonet at lists.utsouthwestern.edu> wrote: >>> >>> I just received a letter from Leica Biosystems where they are >> prohibiting the usage of flammable >>> freezing sprays in their cryostats. What are others using in their >> cryostats to instantly freeze specimens? >>> >>> Thank you. >>> >>> >>> Deanne Knutson >>> Supervisor >>> Anatomic Pathology >>> dknutson at primecare.org >>> >>> >>> >>> >>> >>> >>> ________________________________ >>> This email may include confidential and privileged information. If this >> is not intended for your use, please destroy immediately and contact the >> sender of the message. >>> >>> This email and attachments contain information that may be confidential >> or privileged. If you are not the intended recipient, notify the sender at >> once and delete this message completely from your information system. >> Further use, disclosure, or copying of information contained in this email >> is not authorized, and any such action should not be construed as a waiver >> of privilege or other confidentiality protections. >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ------------------------------ >> >> Message: 3 >> Date: Wed, 25 Sep 2019 15:49:00 +0000 >> From: "Hannen, Valerie" >> To: "'Knutson, Deanne'" >> Cc: "Histonet at lists.utsouthwestern.edu" >> >> Subject: Re: [Histonet] Flammable Sprays in Cryostats >> Message-ID: >> <68e18a8b516a49d4b033de54e175687d at PMSVRLEX02.parrishmed.local> >> Content-Type: text/plain; charset="us-ascii" >> >> We received the same letter months ago. We changed to a non-flammable >> spray. It is called STATFREEZE, we purchase it from STATLAB. The catalog >> number is SL202/134a. Be careful if you order it because they also sell a >> product called STATFREEZE that is flammable. >> >> >> >> Valerie Hannen,MLT(ASCP),HTL,SU (FL) >> Section Chief, Histology >> Parrish Medical Center >> 951 N. Washington Ave. >> Titusville,Florida 32796 >> T: (321)268-6333 ext. 7506 >> F: (321) 268-6149 >> valerie.hannen at parrishmed.com >> www.parrishmed.com >> >> >> >> -----Original Message----- >> From: Knutson, Deanne via Histonet [mailto: >> histonet at lists.utsouthwestern.edu] >> Sent: Wednesday, September 25, 2019 8:51 AM >> To: 'histonet at lists.utsouthwestern.edu' >> Subject: [EXTERNAL Sender] [Histonet] Flammable Sprays in Cryostats >> >> >> This message came from an external source. Please do not click links or >> open attachments if unexpected or unusual. >> >> Begin Original Message: >> >> ---------------------------------------------------------------------- >> I just received a letter from Leica Biosystems where they are prohibiting >> the usage of flammable >> >> freezing sprays in their cryostats. What are others using in their >> cryostats to instantly freeze specimens? >> >> >> >> Thank you. >> >> >> >> >> >> Deanne Knutson >> >> Supervisor >> >> Anatomic Pathology >> >> dknutson at primecare.org >> >> >> >> >> >> >> >> >> >> >> >> >> >> ________________________________ >> >> This email may include confidential and privileged information. If this is >> not intended for your use, please destroy immediately and contact the >> sender of the message. >> >> >> >> This email and attachments contain information that may be confidential or >> privileged. If you are not the intended recipient, notify the sender at >> once and delete this message completely from your information system. >> Further use, disclosure, or copying of information contained in this email >> is not authorized, and any such action should not be construed as a waiver >> of privilege or other confidentiality protections. >> >> _______________________________________________ >> >> Histonet mailing list >> >> Histonet at lists.utsouthwestern.edu >> >> >> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=Xk0H5Lt1SX4yLDi3Z36FWCjxWMexCFmv9kQz5nprqM4&r=S0NRG57vjoVygxIXrxrniUhxWkoaXBwDrgVZ_u7KUmY&m=rqHf4UNcHbvp592ujkRAO5WLlEJw9KUFgBy00GsLhqs&s=aDgPVL1SLa_CnToePuo4VME9QjJOweGzyLEoVKJSWPg&e= >> >> >> >> >> ------------------------------ >> >> Message: 4 >> Date: Wed, 25 Sep 2019 16:42:30 +0000 >> From: Ingles Claire >> To: "'histonet at lists.utsouthwestern.edu'" >> , "Knutson, Deanne" >> >> Subject: Re: [Histonet] Flammable Sprays in Cryostats >> Message-ID: >> < >> DM5PR1701MB191313D76EF3A406C8C73F82C9870 at DM5PR1701MB1913.namprd17.prod.outlook.com >>> >> >> Content-Type: text/plain; charset="us-ascii" >> >> Liquid Nitrogen guns. >> Claire >> ________________________________ >> From: Knutson, Deanne via Histonet >> Sent: Wednesday, September 25, 2019 7:50 AM >> To: 'histonet at lists.utsouthwestern.edu' >> >> Subject: [Histonet] Flammable Sprays in Cryostats >> >> WARNING: This email appears to have originated outside of the UW Health >> email system. >> DO NOT CLICK on links or attachments unless you recognize the sender and >> know the content is safe. >> >> >> >> >> I just received a letter from Leica Biosystems where they are prohibiting >> the usage of flammable >> freezing sprays in their cryostats. What are others using in their >> cryostats to instantly freeze specimens? >> >> Thank you. >> >> >> Deanne Knutson >> Supervisor >> Anatomic Pathology >> dknutson at primecare.org >> >> >> >> >> >> >> ________________________________ >> This email may include confidential and privileged information. If this is >> not intended for your use, please destroy immediately and contact the >> sender of the message. >> >> This email and attachments contain information that may be confidential or >> privileged. If you are not the intended recipient, notify the sender at >> once and delete this message completely from your information system. >> Further use, disclosure, or copying of information contained in this email >> is not authorized, and any such action should not be construed as a waiver >> of privilege or other confidentiality protections. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------ >> >> Subject: Digest Footer >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ------------------------------ >> >> End of Histonet Digest, Vol 190, Issue 18 >> ***************************************** >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jasonhauser71 at gmail.com Wed Sep 25 21:16:48 2019 From: jasonhauser71 at gmail.com (jasonhauser71 at gmail.com) Date: Wed, 25 Sep 2019 22:16:48 -0400 Subject: [Histonet] Flammable Sprays in Cryostats Message-ID: There are non flammable sprays available. We got the recall notice and switched our old inventory out. I found 3 different histology specific sprays. The electronic industry offers some as well. Another option is just using LN2 .Jason HauserSenior Histology TechnicianThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: AkemiDate: Wed, Sep 25, 2019 10:05 AMTo: Knutson, Deanne;Histonet;Cc: Subject:Re: [Histonet] Flammable Sprays in CryostatsWe are purchasing two of their newer UV cryostats and they were adamant that we use a non flammable freezing spray because it can damage the UV unit and have potential for exploding. Akemi Allison Sent from my iPhone > On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet wrote: > > I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable > freezing sprays in their cryostats. What are others using in their cryostats to instantly freeze specimens? > > Thank you. > > > Deanne Knutson > Supervisor > Anatomic Pathology > dknutson at primecare.org > > > > > > > ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Wed Sep 25 22:17:30 2019 From: patpxs at gmail.com (P Sicurello) Date: Wed, 25 Sep 2019 20:17:30 -0700 Subject: [Histonet] Flammable Sprays in Cryostats In-Reply-To: References: Message-ID: It's not safe to use any type of spray in a cryostat. It creates aerosols of who knows what (tuberculosis) from potentially infectious patient biopsies. I would not recommend it, just as a universal precaution. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Wed, Sep 25, 2019 at 7:16 PM jasonhauser71--- via Histonet < histonet at lists.utsouthwestern.edu> wrote: > There are non flammable sprays available. We got the recall notice and > switched our old inventory out. I found 3 different histology specific > sprays. The electronic industry offers some as well. Another option is just > using LN2 .Jason HauserSenior Histology TechnicianThe South Bend ClinicSent > from my T-Mobile 4G LTE device------ Original message------From: AkemiDate: > Wed, Sep 25, 2019 10:05 AMTo: Knutson, Deanne;Histonet;Cc: Subject:Re: > [Histonet] Flammable Sprays in CryostatsWe are purchasing two of their > newer UV cryostats and they were adamant that we use a non flammable > freezing spray because it can damage the UV unit and have potential for > exploding. > > Akemi Allison > > Sent from my iPhone > > > On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet wrote: > > > > I just received a letter from Leica Biosystems where they are > prohibiting the usage of flammable > > freezing sprays in their cryostats. What are others using in their > cryostats to instantly freeze specimens? > > > > Thank you. > > > > > > Deanne Knutson > > Supervisor > > Anatomic Pathology > > dknutson at primecare.org > > > > > > > > > > > > > > ________________________________ > > This email may include confidential and privileged information. If this > is not intended for your use, please destroy immediately and contact the > sender of the message. > > > > This email and attachments contain information that may be confidential > or privileged. If you are not the intended recipient, notify the sender at > once and delete this message completely from your information system. > Further use, disclosure, or copying of information contained in this email > is not authorized, and any such action should not be construed as a waiver > of privilege or other confidentiality protections. > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jasonhauser71 at gmail.com Thu Sep 26 06:37:38 2019 From: jasonhauser71 at gmail.com (jasonhauser71 at gmail.com) Date: Thu, 26 Sep 2019 07:37:38 -0400 Subject: [Histonet] Flammable Sprays in Cryostats Message-ID: <12qurkqhnq3n8cftukqurnh8.1569497733817@email.lge.com> I have heard this before. Any documention of this actually happening.I have tested for blood on surfaces outside the cabinet and never observed a positive result.Jason HauserSenior HistotechThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: P SicurelloDate: Wed, Sep 25, 2019 11:17 PMTo: jasonhauser71 at gmail.com;Cc: Akemi;Knutson, Deanne;Histonet;Subject:Re: [Histonet] Flammable Sprays in CryostatsIt's not safe to use any type of spray in a cryostat.? It creates aerosols of who knows what (tuberculosis) from potentially infectious patient biopsies.? I would not recommend it, just as a universal?precaution.Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health? 9300 Campus Point DriveLa Jolla, CA 92037 (P): 858-249-5610 ? Confidentiality Notice: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer.On Wed, Sep 25, 2019 at 7:16 PM jasonhauser71--- via Histonet wrote:There are non flammable sprays available. We got the recall notice and switched our old inventory out. I found 3 different histology specific sprays. The electronic industry offers some as well. Another option is just using LN2 .Jason HauserSenior Histology TechnicianThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: AkemiDate: Wed, Sep 25, 2019 10:05 AMTo: Knutson, Deanne;Histonet;Cc: Subject:Re: [Histonet] Flammable Sprays in CryostatsWe are purchasing two of their newer UV cryostats and they were adamant that we use a non flammable freezing spray because it can damage the UV unit and have potential for exploding. Akemi Allison Sent from my iPhone > On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet? wrote: > > I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable > freezing sprays in their cryostats.? What are others using in their cryostats to instantly freeze specimens? > > Thank you. > > > Deanne Knutson > Supervisor > Anatomic Pathology > dknutson at primecare.org > > > > > > >? ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Thu Sep 26 06:55:54 2019 From: patpxs at gmail.com (P Sicurello) Date: Thu, 26 Sep 2019 04:55:54 -0700 Subject: [Histonet] Flammable Sprays in Cryostats In-Reply-To: <12qurkqhnq3n8cftukqurnh8.1569497733817@email.lge.com> References: <12qurkqhnq3n8cftukqurnh8.1569497733817@email.lge.com> Message-ID: This was copied from the Centers for Disease Control's Morbidity and Mortality Weekly Report (January 2012) *5.9.2. Work at the open bench* Frozen sections ? Frozen sectioning is performed on fresh tissue and is a high-risk procedure for infectious exposure. Freezing tissue does not kill organisms, and the use of the cryostat cutting blade creates potentially dangerous aerosols. Discuss the true clinical necessity for frozen sectioning with the surgical team. ? Although some cryostat instruments have a downdraft into the instrument, aerosols are dispersed into the room where the cutting takes place. Do not use freezing propellant sprays, which speed the freezing process by a few seconds and cause aerosolization of not only the tissue being frozen but also the tissues from previously cut specimens that are at the base of the instrument. Such procedures generate aerosol and droplet contamination, posing an infectious risk to all personnel in the area (*56,79,82*). The Clinical and Laboratory Standards Institute and others have recommended discontinuation of freezing sprays because they are not recommended by the manufacturers of cryostat instrumentation (*2,79*). Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Thu, Sep 26, 2019 at 4:37 AM jasonhauser71 at gmail.com < jasonhauser71 at gmail.com> wrote: > I have heard this before. Any documention of this actually happening. > I have tested for blood on surfaces outside the cabinet and never observed > a positive result. > > > Jason Hauser > Senior Histotech > The South Bend Clinic > > *Sent from my T-Mobile 4G LTE device* > > ------ Original message------ > *From: *P Sicurello > *Date: *Wed, Sep 25, 2019 11:17 PM > *To: *jasonhauser71 at gmail.com; > *Cc: *Akemi;Knutson, Deanne;Histonet; > *Subject:*Re: [Histonet] Flammable Sprays in Cryostats > > It's not safe to use any type of spray in a cryostat. It creates aerosols > of who knows what (tuberculosis) from potentially infectious patient > biopsies. I would not recommend it, just as a universal precaution. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 9300 Campus Point Drive > > La Jolla, CA 92037 > (P): 858-249-5610 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > > > On Wed, Sep 25, 2019 at 7:16 PM jasonhauser71--- via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> There are non flammable sprays available. We got the recall notice and >> switched our old inventory out. I found 3 different histology specific >> sprays. The electronic industry offers some as well. Another option is just >> using LN2 .Jason HauserSenior Histology TechnicianThe South Bend ClinicSent >> from my T-Mobile 4G LTE device------ Original message------From: AkemiDate: >> Wed, Sep 25, 2019 10:05 AMTo: Knutson, Deanne;Histonet;Cc: Subject:Re: >> [Histonet] Flammable Sprays in CryostatsWe are purchasing two of their >> newer UV cryostats and they were adamant that we use a non flammable >> freezing spray because it can damage the UV unit and have potential for >> exploding. >> >> Akemi Allison >> >> Sent from my iPhone >> >> > On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet wrote: >> > >> > I just received a letter from Leica Biosystems where they are >> prohibiting the usage of flammable >> > freezing sprays in their cryostats. What are others using in their >> cryostats to instantly freeze specimens? >> > >> > Thank you. >> > >> > >> > Deanne Knutson >> > Supervisor >> > Anatomic Pathology >> > dknutson at primecare.org >> > >> > >> > >> > >> > >> > >> > ________________________________ >> > This email may include confidential and privileged information. If this >> is not intended for your use, please destroy immediately and contact the >> sender of the message. >> > >> > This email and attachments contain information that may be confidential >> or privileged. If you are not the intended recipient, notify the sender at >> once and delete this message completely from your information system. >> Further use, disclosure, or copying of information contained in this email >> is not authorized, and any such action should not be construed as a waiver >> of privilege or other confidentiality protections. >> > _______________________________________________ >> > Histonet mailing list >> > Histonet at lists.utsouthwestern.edu >> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From Karen.Heckford at DignityHealth.org Thu Sep 26 06:57:03 2019 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Thu, 26 Sep 2019 11:57:03 +0000 Subject: [Histonet] HT per diem position in San Francisco Message-ID: <3552561049cb40a4bec726e6825d0200@PHX-EXCH-013.chw.edu> Good Morning, We are actively looking for a per diem Histology Technician to cover for vacations and administration days. Will more than likely need a person 8 hours a week for now. Must be able to work independently without supervision. We hand do special stains. IHC experience is preferred. If you have any questions please do not hesitate to call me Karen Heckford at 415-668-1000 extension 6167. This position could possibly develop into a part time position later. The hours are 430am to 1pm but can discuss working around your schedule if needed. Please go to Dignity Health St. Mary's Medical Center San Francisco to apply. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you From jasonhauser71 at gmail.com Thu Sep 26 08:00:54 2019 From: jasonhauser71 at gmail.com (jasonhauser71 at gmail.com) Date: Thu, 26 Sep 2019 09:00:54 -0400 Subject: [Histonet] Flammable Sprays in Cryostats Message-ID: <7m9v0hi0kcc9ilpou4kh4405.1569502796078@email.lge.com> I woukd like the link to that. I looked around a bit on the web but couldnt locate usinf search.Jason HauserSenior HistotechnologistThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: P SicurelloDate: Thu, Sep 26, 2019 7:56 AMTo: jasonhauser71 at gmail.com;Cc: Akemi;Knutson, Deanne;Histonet;Subject:Re: [Histonet] Flammable Sprays in CryostatsThis was copied from the Centers for Disease Control's Morbidity and Mortality Weekly Report (January 2012)5.9.2. Work at the open benchFrozen sections? Frozen sectioning is performed on fresh tissue and is a high-risk procedure for infectious exposure. Freezing tissue does not kill organisms, and the use of the cryostat cutting blade creates potentially dangerous aerosols. Discuss the true clinical necessity for frozen sectioning with the surgical team.? Although some cryostat instruments have a downdraft into the instrument, aerosols are dispersed into the room where the cutting takes place. Do not use freezing propellant sprays, which speed the freezing process by a few seconds and cause aerosolization of not only the tissue being frozen but also the tissues from previously cut specimens that are at the base of the instrument. Such procedures generate aerosol and droplet contamination, posing an infectious risk to all personnel in the area (56,79,82). The Clinical and Laboratory Standards Institute and others have recommended discontinuation of freezing sprays because they are not recommended by the manufacturers of cryostat instrumentation (2,79).??Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health? 9300 Campus Point DriveLa Jolla, CA 92037 (P): 858-249-5610 ? Confidentiality Notice: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer.On Thu, Sep 26, 2019 at 4:37 AM jasonhauser71 at gmail.com wrote: I have heard this before. Any documention of this actually happening.I have tested for blood on surfaces outside the cabinet and never observed a positive result.Jason HauserSenior HistotechThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: P SicurelloDate: Wed, Sep 25, 2019 11:17 PMTo: jasonhauser71 at gmail.com;Cc: Akemi;Knutson, Deanne;Histonet;Subject:Re: [Histonet] Flammable Sprays in CryostatsIt's not safe to use any type of spray in a cryostat.? It creates aerosols of who knows what (tuberculosis) from potentially infectious patient biopsies.? I would not recommend it, just as a universal?precaution.Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health? 9300 Campus Point DriveLa Jolla, CA 92037 (P): 858-249-5610 ? Confidentiality Notice: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer.On Wed, Sep 25, 2019 at 7:16 PM jasonhauser71--- via Histonet wrote:There are non flammable sprays available. We got the recall notice and switched our old inventory out. I found 3 different histology specific sprays. The electronic industry offers some as well. Another option is just using LN2 .Jason HauserSenior Histology TechnicianThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: AkemiDate: Wed, Sep 25, 2019 10:05 AMTo: Knutson, Deanne;Histonet;Cc: Subject:Re: [Histonet] Flammable Sprays in CryostatsWe are purchasing two of their newer UV cryostats and they were adamant that we use a non flammable freezing spray because it can damage the UV unit and have potential for exploding. Akemi Allison Sent from my iPhone > On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet? wrote: > > I just received a letter from Leica Biosystems where they are prohibiting the usage of flammable > freezing sprays in their cryostats.? What are others using in their cryostats to instantly freeze specimens? > > Thank you. > > > Deanne Knutson > Supervisor > Anatomic Pathology > dknutson at primecare.org > > > > > > >? ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lisa.White3 at va.gov Thu Sep 26 08:32:11 2019 From: Lisa.White3 at va.gov (White, Lisa M.) Date: Thu, 26 Sep 2019 13:32:11 +0000 Subject: [Histonet] Histonet] Freezing spray in cryostats Message-ID: We did use freeze spray in the cryostat years ago. Then one fateful day we all had to go on protocol for exposure to TB because a resident sprayed a lung with the freeze spray and there you go. There is a turn around time for frozen sections we are all working with, HOWEVER it is not worth the health and safety of Staff to rush the process. Use the heat extractor and most cryostats have a "turbo" cool bar. Safety first or no one will be left to run the lab. From Christopher.Sheeder at seattlechildrens.org Thu Sep 26 10:00:23 2019 From: Christopher.Sheeder at seattlechildrens.org (Sheeder, Christopher) Date: Thu, 26 Sep 2019 15:00:23 +0000 Subject: [Histonet] Flammable Sprays in Cryostats In-Reply-To: <7m9v0hi0kcc9ilpou4kh4405.1569502796078@email.lge.com> References: <7m9v0hi0kcc9ilpou4kh4405.1569502796078@email.lge.com> Message-ID: <4a820440d732425fbb6fd92b506d436f@seattlechildrens.org> I would like the link as well, if possible. Christopher Sheeder, HT(ASCP)QIHC Histology Supervisor | Department of Laboratories Seattle Children?s Hospital 4800 Sand Point Way NE Seattle, WA 98105 -----Original Message----- From: jasonhauser71 at gmail.com Sent: Thursday, September 26, 2019 6:01 AM To: P Sicurello Cc: Akemi ; Knutson, Deanne ; Histonet Subject: Re: [Histonet] Flammable Sprays in Cryostats I woukd like the link to that. I looked around a bit on the web but couldnt locate usinf search.Jason HauserSenior HistotechnologistThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: P SicurelloDate: Thu, Sep 26, 2019 7:56 AMTo: jasonhauser71 at gmail.com;Cc: Akemi;Knutson, Deanne;Histonet;Subject:Re: [Histonet] Flammable Sprays in CryostatsThis was copied from the Centers for Disease Control's Morbidity and Mortality Weekly Report (January 2012)5.9.2. Work at the open benchFrozen sections? Frozen sectioning is performed on fresh tissue and is a high-risk procedure for infectious exposure. Freezing tissue does not kill organisms, and the use of the cryostat cutting blade creates potentially dangerous aerosols. Discuss the true clinical necessity for frozen sectioning with the surgical team.? Although some cryostat instruments have a downdraft into the instrument, aerosols are dispersed into the room where the cutting takes place. Do not use freezing propellant sprays, which speed the freezing process by a few seconds and cause aerosolization of not only the tissue being frozen but also the tissues from previously cut specimens that are at the base of the instrument. Such procedures generate aerosol and droplet contamination, posing an infectious risk to all personnel in the area (56,79,82). The Clinical and Laboratory Standards Institute and others have recommended discontinuation of freezing sprays because they are not recommended by the manufacturers of cryostat instrumentation (2,79). Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point DriveLa Jolla, CA 92037 (P): 858-249-5610 Confidentiality Notice: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer.On Thu, Sep 26, 2019 at 4:37 AM jasonhauser71 at gmail.com wrote: I have heard this before. Any documention of this actually happening.I have tested for blood on surfaces outside the cabinet and never observed a positive result.Jason HauserSenior HistotechThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: P SicurelloDate: Wed, Sep 25, 2019 11:17 PMTo: jasonhauser71 at gmail.com;Cc: Akemi;Knutson, Deanne;Histonet;Subject:Re: [Histonet] Flammable Sprays in CryostatsIt's not safe to use any type of spray in a cryostat. It creates aerosols of who knows what (tuberculosis) from potentially infectious patient biopsies. I would not recommend it, just as a universal precaution.Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point DriveLa Jolla, CA 92037 (P): 858-249-5610 Confidentiality Notice: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer.On Wed, Sep 25, 2019 at 7:16 PM jasonhauser71--- via Histonet wrote:There are non flammable sprays available. We got the recall notice and switched our old inventory out. I found 3 different histology specific sprays. The electronic industry offers some as well. Another option is just using LN2 .Jason HauserSenior Histology TechnicianThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: AkemiDate: Wed, Sep 25, 2019 10:05 AMTo: Knutson, Deanne;Histonet;Cc: Subject:Re: [Histonet] Flammable Sprays in CryostatsWe are purchasing two of their newer UV cryostats and they were adamant that we use a non flammable freezing spray because it can damage the UV unit and have potential for exploding. Akemi Allison Sent from my iPhone > On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet wrote: > > I just received a letter from Leica Biosystems where they are > prohibiting the usage of flammable freezing sprays in their cryostats. What are others using in their cryostats to instantly freeze specimens? > > Thank you. > > > Deanne Knutson > Supervisor > Anatomic Pathology > dknutson at primecare.org > > > > > > > ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=DwIDaQ&c=aBkXpkKi7gN5fe5MqrMaN-VmRu > gaRb1IDRfSv2xVRy0&r=c3bFZxewNEDI2wFyTyj_1jhuE1dV-f-i0gIzQyuiCw3aEzfs6m > Qm_0c0YpHyN0fx&m=ZufLa6S-dAQ4lIgHiq9fe2PcBZJVEPiC53w8R519I48&s=Zjuo48k > 9i7oCN2jiPtxMU3GdhBc5kdQV-hi7y_7tSZw&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIDaQ&c=aBkXpkKi7gN5fe5MqrMaN-VmRugaRb1IDRfSv2xVRy0&r=c3bFZxewNEDI2wFyTyj_1jhuE1dV-f-i0gIzQyuiCw3aEzfs6mQm_0c0YpHyN0fx&m=ZufLa6S-dAQ4lIgHiq9fe2PcBZJVEPiC53w8R519I48&s=Zjuo48k9i7oCN2jiPtxMU3GdhBc5kdQV-hi7y_7tSZw&e= CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Timothy.Morken at ucsf.edu Thu Sep 26 10:29:35 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 26 Sep 2019 15:29:35 +0000 Subject: [Histonet] Reference website RE: Flammable Sprays in Cryostats Message-ID: You can see past issues of Morbidly and Mortality Weekly Report (MMWR) at: https://www.cdc.gov/mmwr/index2019.html For past issues, click on "weekly report" on the left hand side and you can get to a list of past issues. However, for the publication about cryostats, look under "Supplements" a bit lower on the page and choose the 2012 supplement issue (Vol 61) . It is in the one on Laboratory Safety. It has a lot more than just cryostat safety. https://www.cdc.gov/mmwr/preview/ind2012_su.html Scroll to the very bottom of the page to see the January supplement The full reference is: MMWR January 6, 2012 / Vol. 61 / Supplement / Pg. 1 - 105 Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Sheeder, Christopher via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 26, 2019 8:00 AM To: jasonhauser71 at gmail.com; P Sicurello Cc: Histonet Subject: Re: [Histonet] Flammable Sprays in Cryostats I would like the link as well, if possible. Christopher Sheeder, HT(ASCP)QIHC Histology Supervisor | Department of Laboratories Seattle Children?s Hospital 4800 Sand Point Way NE Seattle, WA 98105 -----Original Message----- From: jasonhauser71 at gmail.com Sent: Thursday, September 26, 2019 6:01 AM To: P Sicurello Cc: Akemi ; Knutson, Deanne ; Histonet Subject: Re: [Histonet] Flammable Sprays in Cryostats I woukd like the link to that. I looked around a bit on the web but couldnt locate usinf search.Jason HauserSenior HistotechnologistThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: P SicurelloDate: Thu, Sep 26, 2019 7:56 AMTo: jasonhauser71 at gmail.com;Cc: Akemi;Knutson, Deanne;Histonet;Subject:Re: [Histonet] Flammable Sprays in CryostatsThis was copied from the Centers for Disease Control's Morbidity and Mortality Weekly Report (January 2012)5.9.2. Work at the open benchFrozen sections? Frozen sectioning is performed on fresh tissue and is a high-risk procedure for infectious exposure. Freezing tissue does not kill organisms, and the use of the cryostat cutting blade creates potentially dangerous aerosols. Discuss the true clinical necessity for frozen sectioning with the surgical team.? Although some cryostat instruments have a downdraft into the instrument, aerosols are dispersed into the room where the cutting takes place. Do not use freezing propellant sprays, which speed the freezing process by a few seconds and cause aerosolization of not only the tissue being frozen but also the tissues from previously cut specimens that are at the base of the instrument. Such procedures generate aerosol and droplet contamination, posing an infectious risk to all personnel in the area (56,79,82). The Clinical and Laboratory Standards Institute and others have recommended discontinuation of freezing sprays because they are not recommended by the manufacturers of cryostat instrumentation (2,79). Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point DriveLa Jolla, CA 92037 (P): 858-249-5610 Confidentiality Notice: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer.On Thu, Sep 26, 2019 at 4:37 AM jasonhauser71 at gmail.com wrote: I have heard this before. Any documention of this actually happening.I have tested for blood on surfaces outside the cabinet and never observed a positive result.Jason HauserSenior HistotechThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: P SicurelloDate: Wed, Sep 25, 2019 11:17 PMTo: jasonhauser71 at gmail.com;Cc: Akemi;Knutson, Deanne;Histonet;Subject:Re: [Histonet] Flammable Sprays in CryostatsIt's not safe to use any type of spray in a cryostat. It creates aerosols of who knows what (tuberculosis) from potentially infectious patient biopsies. I would not recommend it, just as a universal precaution.Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point DriveLa Jolla, CA 92037 (P): 858-249-5610 Confidentiality Notice: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer.On Wed, Sep 25, 2019 at 7:16 PM jasonhauser71--- via Histonet wrote:There are non flammable sprays available. We got the recall notice and switched our old inventory out. I found 3 different histology specific sprays. The electronic industry offers some as well. Another option is just using LN2 .Jason HauserSenior Histology TechnicianThe South Bend ClinicSent from my T-Mobile 4G LTE device------ Original message------From: AkemiDate: Wed, Sep 25, 2019 10:05 AMTo: Knutson, Deanne;Histonet;Cc: Subject:Re: [Histonet] Flammable Sprays in CryostatsWe are purchasing two of their newer UV cryostats and they were adamant that we use a non flammable freezing spray because it can damage the UV unit and have potential for exploding. Akemi Allison Sent from my iPhone > On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet wrote: > > I just received a letter from Leica Biosystems where they are > prohibiting the usage of flammable freezing sprays in their cryostats. What are others using in their cryostats to instantly freeze specimens? > > Thank you. > > > Deanne Knutson > Supervisor > Anatomic Pathology > dknutson at primecare.org > > > > > > > ________________________________ > This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. > > This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=DwIDaQ&c=aBkXpkKi7gN5fe5MqrMaN-VmRu > gaRb1IDRfSv2xVRy0&r=c3bFZxewNEDI2wFyTyj_1jhuE1dV-f-i0gIzQyuiCw3aEzfs6m > Qm_0c0YpHyN0fx&m=ZufLa6S-dAQ4lIgHiq9fe2PcBZJVEPiC53w8R519I48&s=Zjuo48k > 9i7oCN2jiPtxMU3GdhBc5kdQV-hi7y_7tSZw&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIDaQ&c=aBkXpkKi7gN5fe5MqrMaN-VmRugaRb1IDRfSv2xVRy0&r=c3bFZxewNEDI2wFyTyj_1jhuE1dV-f-i0gIzQyuiCw3aEzfs6mQm_0c0YpHyN0fx&m=ZufLa6S-dAQ4lIgHiq9fe2PcBZJVEPiC53w8R519I48&s=Zjuo48k9i7oCN2jiPtxMU3GdhBc5kdQV-hi7y_7tSZw&e= CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=TcHKAVvHTOSJdb71EpxZ2mlacC5XMcFYzuAOI8mNLAk&s=czUVC25-fEmIjL3WpVRj3dMOnr-wHz1UsEw4VHHyPkc&e= From rsrichmond at gmail.com Thu Sep 26 11:05:28 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 26 Sep 2019 12:05:28 -0400 Subject: [Histonet] Freezing Sprays in Cryostats In-Reply-To: References: Message-ID: Freezing sprays for frozen sections in cryostats are deplorable, but try and get pathologists to give them up. I think that's probably why the CAP has been reluctant to ban them. During my career in pathology I saw more than one case where frozen sections were inadvertently cut on tuberculous tissue. Tuberculosis is a disease that sneaks up on you. In Baltimore (a hotbed of the disease) around 1970 when we did this, we'd put the cryostat (one of the basic Damon IEC models we had then) out of action until somebody (usually the chief resident) could bring it up to room temperature, scrub it out with alcohol, lubricate it, and plug it back in to cool down. The heat extractors are usually all you need. Liquid nitrogen is not a very satisfactory freezing medium. It's better to submerge a freezing medium liquid into liquid nitrogen (which will eventually freeze it solid, however). Shandon used to market a freezing container called a Histobath - is any equivalent product available today? It held the right temperature not to freeze the liquid medium solid. People usually use acetone or isopentane (2-methylbutane) as the freezing medium, both highly flammable. Better is: 3M? Novec? Engineered Fluid HFE-7100 This product belongs to a class of fluorocarbons called "segregated hydrofluoroethers (HFE's)" According to various MSDS, HFE-7100 is methyl nonafluoroisobutyl ether C4F9-O-CH3 But try to substitute any such thing for freezing spray, and be prepared for a hissy-fit from your senior pathologist. I'm glad the CDC weighed in on this. I do remind them that the decision to do a frozen section is between the surgeon and the pathologist. Bob Richmond Samurai Pathologist Maryville TN From Timothy.Morken at ucsf.edu Thu Sep 26 11:36:24 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 26 Sep 2019 16:36:24 +0000 Subject: [Histonet] Freezing Sprays in Cryostats In-Reply-To: References: Message-ID: I wonder if it is just a coincidence that and ad for this device from Milestone came thru in the middle of this freezing spray string.... https://www.milestonemedsrl.com/us/product/prestochill/ Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 26, 2019 9:05 AM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Freezing Sprays in Cryostats Freezing sprays for frozen sections in cryostats are deplorable, but try and get pathologists to give them up. I think that's probably why the CAP has been reluctant to ban them. During my career in pathology I saw more than one case where frozen sections were inadvertently cut on tuberculous tissue. Tuberculosis is a disease that sneaks up on you. In Baltimore (a hotbed of the disease) around 1970 when we did this, we'd put the cryostat (one of the basic Damon IEC models we had then) out of action until somebody (usually the chief resident) could bring it up to room temperature, scrub it out with alcohol, lubricate it, and plug it back in to cool down. The heat extractors are usually all you need. Liquid nitrogen is not a very satisfactory freezing medium. It's better to submerge a freezing medium liquid into liquid nitrogen (which will eventually freeze it solid, however). Shandon used to market a freezing container called a Histobath - is any equivalent product available today? It held the right temperature not to freeze the liquid medium solid. People usually use acetone or isopentane (2-methylbutane) as the freezing medium, both highly flammable. Better is: 3M? Novec? Engineered Fluid HFE-7100 This product belongs to a class of fluorocarbons called "segregated hydrofluoroethers (HFE's)" According to various MSDS, HFE-7100 is methyl nonafluoroisobutyl ether C4F9-O-CH3 But try to substitute any such thing for freezing spray, and be prepared for a hissy-fit from your senior pathologist. I'm glad the CDC weighed in on this. I do remind them that the decision to do a frozen section is between the surgeon and the pathologist. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=R6ly1aYBux_iTkMzQFNAafzWQp9O1m03MG5R-w28Glo&s=ToFRiODn2aNAFefsFbNQXdqihIaIEqcFgRBb6I_9UuA&e= From pathgroupfe1 at gmail.com Thu Sep 26 12:06:15 2019 From: pathgroupfe1 at gmail.com (Pathgroup MD) Date: Thu, 26 Sep 2019 12:06:15 -0500 Subject: [Histonet] Used lab equipment available Message-ID: Is anyone looking for used cytology or histology equipment for their lab? We have excellent, gently used equipment such as cryostats, tissue baths, stretching tables, stainers, tissue processors, mikrotomes, exhaust hoods, and more. Further information can be found at https://www.medite-group.com/ From akemiat3377 at gmail.com Fri Sep 27 06:59:55 2019 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Fri, 27 Sep 2019 05:59:55 -0600 Subject: [Histonet] Histo techs assisting on BM Bx's Message-ID: <493256D2-B145-427E-8C3D-36CFC4CF773B@gmail.com> Good Morning Histo Peeps: Back from a great trip to NSH NOLA and hit the ground running yesterday. I am faced with a department coverage issue which impacts our TAT. I am curious how many facilities have their histologists assist with BM Bx?s. I have never in my 50 years as a histologist assisted with BM Bx?s. That responsibility was under the hematology department responsibilities. We have 4 FT Histo tech?s and 1 PNR in our department. Yesterday we had 2 histologists pulled away from bench duties to assist with BM Bx?s, and then another histo tech was called to go to the main hospital to cut FS?s, which is 2 blokes away. One of the tech?s was gone for 3 hrs because the pathologist performing the BM was extremely slow. That left 1 tech and myself to cover bench duties. Keep in mind this is a county hospital with limited funding and a freeze on staffing. Thank you in advance for your input. Akemi Akemi Allison, BS, HT/HTL Histology Supervisor UMC El Paso, TX From POWELL_SA at mercer.edu Fri Sep 27 09:38:25 2019 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Fri, 27 Sep 2019 14:38:25 +0000 Subject: [Histonet] Freezing Sprays in Cryostats Message-ID: I agree with not using a spray in cryostats. We had to get tested a few times in the hospital for TB after discovering the case was positive after the fact, scary. This is the same as flushing the toilet with the seat open. I know that sounds crazy but here is just one article of many online that explains the aerosol effect. https://www.realtor.com/advice/home-improvement/flushing-the-toilet-with-the-lid-up-what-happens/ Please don't send me criticism for posting this, it is real. Read the articles. It is a good reason to stop using sprays in the cryostat. Shirley Powell, HTL (ASCP) Technical Director Histology Curricular Support Lab Pathology Mercer University School of Medicine 1550 College Street | Macon, GA | 31207 O:478-301-2374| F:478-301-5489 medicine.mercer.edu. -----Original Message----- From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 26, 2019 12:05 PM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Freezing Sprays in Cryostats Freezing sprays for frozen sections in cryostats are deplorable, but try and get pathologists to give them up. I think that's probably why the CAP has been reluctant to ban them. During my career in pathology I saw more than one case where frozen sections were inadvertently cut on tuberculous tissue. Tuberculosis is a disease that sneaks up on you. In Baltimore (a hotbed of the disease) around 1970 when we did this, we'd put the cryostat (one of the basic Damon IEC models we had then) out of action until somebody (usually the chief resident) could bring it up to room temperature, scrub it out with alcohol, lubricate it, and plug it back in to cool down. The heat extractors are usually all you need. Liquid nitrogen is not a very satisfactory freezing medium. It's better to submerge a freezing medium liquid into liquid nitrogen (which will eventually freeze it solid, however). Shandon used to market a freezing container called a Histobath - is any equivalent product available today? It held the right temperature not to freeze the liquid medium solid. People usually use acetone or isopentane (2-methylbutane) as the freezing medium, both highly flammable. Better is: 3M? Novec? Engineered Fluid HFE-7100 This product belongs to a class of fluorocarbons called "segregated hydrofluoroethers (HFE's)" According to various MSDS, HFE-7100 is methyl nonafluoroisobutyl ether C4F9-O-CH3 But try to substitute any such thing for freezing spray, and be prepared for a hissy-fit from your senior pathologist. I'm glad the CDC weighed in on this. I do remind them that the decision to do a frozen section is between the surgeon and the pathologist. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cpowell_sa%40mercer.edu%7C6ea7b78385694702d99208d7429b803f%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C637051107953175885&sdata=%2FghvEuBLQ5fxVJkf9VvdDd0AzPo8ycBAS5vzc31U8H0%3D&reserved=0 From twebster at CRH.org Fri Sep 27 12:13:29 2019 From: twebster at CRH.org (Webster, Thomas S.) Date: Fri, 27 Sep 2019 17:13:29 +0000 Subject: [Histonet] Histo techs assisting on BM Bx's Message-ID: <95813808fc50463bbf57f357b2ce6c8f@CRH.org> Our histotechs assist bone marrows but it used to be Hematology's responsibility. The bone marrows at my institution aren't very time consuming and are done in CT. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From rsrichmond at gmail.com Sat Sep 28 14:50:33 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 28 Sep 2019 15:50:33 -0400 Subject: [Histonet] Histo techs assisting on BM Bx's In-Reply-To: References: Message-ID: I usually had hematology technologists assisting me back when I was doing bone marrow biopsies long ago. I trained more technologists than I remember. You always have to remind the patient that you're going to be talking a lot because you're training a new assistant, but that it isn't going to make any difference to the patient. This is one of those situations where too many docs (particularly pathologists) pitch hissy-fits, and make the procedure difficult for the assistant. Bone marrow biopsy with CT guidance - boggles the mind. Pathologists had pretty well retired from doing bone marrow biopsies by the time CT guidance became available - hematologists get paid so much more for doing them than pathologists do that they pretty well took the procedure over. I'd rather do it myself - I get big specimens properly prepared that way. I still have a couple of those old 8-gauge Westerman-Jensen needles. Bob Richmond Samurai Pathologist Maryville TN From jqb7 at cdc.gov Mon Sep 30 05:49:04 2019 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)) Date: Mon, 30 Sep 2019 10:49:04 +0000 Subject: [Histonet] Metals Message-ID: Morning all! I need some advice re: protocols to demonstrate metals in FFPE tissues. Metals such as copper, aluminum and zinc. Thanks much! Jeanine Sanders, BS, HT(ASCP), QIHC(ASCP) Centers for Diseases Control and Prevention 1600 Clifton Road NE MS H18-SB Bldg. 18, Rm SB-114 Atlanta, GA 30329 404-639-3590 From tony.henwood at health.nsw.gov.au Mon Sep 30 07:22:42 2019 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Mon, 30 Sep 2019 12:22:42 +0000 Subject: [Histonet] Metals In-Reply-To: References: Message-ID: <1569846163180.61@health.nsw.gov.au> Two good screening stains are Mallory and Parker?s Fresh Hematoxylin Stain for Metals and Timm?s Silver Sulphide Method for Metals. Malloy's results: Aluminium Blue-black Copper Greenish-blue Iron Blue-black Lead Blue Zinc Blue For more specific staining: Aluminon Stain for Aluminium Hydroxide Walton?s Stain for Aluminium (Phloxine binds the aluminium) Bedrick et al (1986) method for Zinc Rubeanic Acid Technique for Copper Rhodanine Technique for Copper These methods are quite sensitive but there are some specificity issues. I can provide further details and references if required. Here are some: Ohtsuki, Y., Yamaguchi, T., Sonobe, H., Takahashi, K., Hayashi, K., Takenaka, A., ... & Terao, N. (1989). Stain Technology: A Simplified Aluminum Stain in Paraffin Sections of Bone from Hemodialysis Patients. Stain technology, 64(2), 55-59. Walton, J. R., Diamond, T. H., Kumar, S., & Murrell, G. A. C. (2007). A sensitive stain for aluminum in undecalcified cancellous bone. Journal of inorganic biochemistry, 101(9), 1285-1290. Bedrick, A. E., Ramaswamy, G., & Tchertkoff, V. (1986). Histochemical determination of copper, zinc, and iron in some benign and malignant tissues. American journal of clinical pathology, 86(5), 637-640. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet Sent: Monday, 30 September 2019 20:49 To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Metals Morning all! I need some advice re: protocols to demonstrate metals in FFPE tissues. Metals such as copper, aluminum and zinc. Thanks much! Jeanine Sanders, BS, HT(ASCP), QIHC(ASCP) Centers for Diseases Control and Prevention 1600 Clifton Road NE MS H18-SB Bldg. 18, Rm SB-114 Atlanta, GA 30329 404-639-3590 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From tbraud at holyredeemer.com Mon Sep 30 08:30:52 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 30 Sep 2019 13:30:52 +0000 Subject: [Histonet] Bone Marrow Assists Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B609B4@HRHEX02-HOS.holyredeemer.local> Our Histology Techs also assist with Bone Marrows in CT, though there is little to do. We collect the aspirate in Heparin Tubes and the core in Bouins. We bring it back to the lab and make our smears from one of the heparin tubes. The other tubes go for special studies. I'd like to think our smears are better than many. We pour the aspirate into a weigh boat and use a pipet to pick out the spicules for picture perfect smears. Much better results than the old method of making the smears at bedside. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Saturday, September 28, 2019 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 190, Issue 22 Today's Topics: 1. Re: Histo techs assisting on BM Bx's (Webster, Thomas S.) ---------------------------------------------------------------------- Message: 1 Date: Fri, 27 Sep 2019 17:13:29 +0000 From: "Webster, Thomas S." Subject: Re: [Histonet] Histo techs assisting on BM Bx's Our histotechs assist bone marrows but it used to be Hematology's responsibility. The bone marrows at my institution aren't very time consuming and are done in CT. From aperl at caremount.com Mon Sep 30 09:24:36 2019 From: aperl at caremount.com (Perl, Alison) Date: Mon, 30 Sep 2019 14:24:36 +0000 Subject: [Histonet] job opportunity In-Reply-To: <99e35bedc6bc4479baaefd58c77019ef@MK-EXMB02.mkmg.com> References: <99e35bedc6bc4479baaefd58c77019ef@MK-EXMB02.mkmg.com> Message-ID: Hi all If you are in or near Westchester county, NY, we have a couple of openings - FT and PT Histotech, and FT Grossing tech. If you're interested please send me your resume! Check out the links for more info https://recruiting.ultipro.com/CAR1044CMPC/JobBoard/9cce8d9a-cf97-4d25-b397-75950a6bbb49/OpportunityDetail?opportunityId=2e52bbcc-97b5-472e-8fa0-2e9acb512b61 https://recruiting.ultipro.com/CAR1044CMPC/JobBoard/9cce8d9a-cf97-4d25-b397-75950a6bbb49/OpportunityDetail?opportunityId=894a4038-de91-4a3f-bedb-37196ab56baa Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical 110 South Bedford Rd Mount Kisco, NY 10549 (914) 302-8424 aperl at caremount.com www.caremountmedical.com