From jkiernan at uwo.ca Wed Oct 2 00:00:43 2019 From: jkiernan at uwo.ca (John Kiernan) Date: Wed, 2 Oct 2019 05:00:43 +0000 Subject: [Histonet] Metals In-Reply-To: <1569846163180.61@health.nsw.gov.au> References: , <1569846163180.61@health.nsw.gov.au> Message-ID: Timm's sulphide-silver method is very sensitive, and modifications (mostly by Danscher) even more so. Sulphide-silver methods detect only those metals that have insoluble sulphides (copper and zinc but not aluminium, in Jeanine's list). It is necessary to fix in a special solution containing hydrogen sulphide - stink and also serious safety precautions! Tony's mention of Mallory & Parker's fresh hematoxylin stain prompted me to look it up. The 1939 paper is a free PDF download (Google Scholar: Mallory Parker Hematoxylin Stain Metals). Mallory FB, Parker F (1939) Fixing and staining methods for lead and copper in tissues. Am. J. Pathol. 15: 517-522 and Plates 83-85. The authors noted the importance of fixation (neutral formalin was OK for copper but no good for lead, which needed 95% or 100% alcohol). Like F.B. Mallory's other papers about staining methods, it's rather vague on technical details and has no references. The late Ralph D. Lillie reported more thorough investigations of staining for metals with haematoxylin in his classic book Histopathologic Technic and Practical Histochemistry (4th and last edn 1976, ISBN 0070378622), giving colours of the complexes with 30 metals introduced into tissues. I have tried Lillie's method on paraffin sections of rat tissues containing a few of these, and it works. ISBN 9781907904325 (p.333-334) may be more accessible than Lillie's book, which has become an expensive classic. For the more specific stains mentioned in Tony's message you need to do some critical reading. The best place to start may be Frieda Carson's Histotechnology textbook. ISBN 978-0891896401. Enough about metals for now! John Kiernan London, Canada = = = ________________________________ From: Tony Henwood (SCHN) via Histonet Sent: 30 September 2019 07:22 To: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) Cc: Histonet Subject: Re: [Histonet] Metals Two good screening stains are Mallory and Parker?s Fresh Hematoxylin Stain for Metals and Timm?s Silver Sulphide Method for Metals. Malloy's results: Aluminium Blue-black Copper Greenish-blue Iron Blue-black Lead Blue Zinc Blue For more specific staining: Aluminon Stain for Aluminium Hydroxide Walton?s Stain for Aluminium (Phloxine binds the aluminium) Bedrick et al (1986) method for Zinc Rubeanic Acid Technique for Copper Rhodanine Technique for Copper These methods are quite sensitive but there are some specificity issues. I can provide further details and references if required. Here are some: Ohtsuki, Y., Yamaguchi, T., Sonobe, H., Takahashi, K., Hayashi, K., Takenaka, A., ... & Terao, N. (1989). Stain Technology: A Simplified Aluminum Stain in Paraffin Sections of Bone from Hemodialysis Patients. Stain technology, 64(2), 55-59. Walton, J. R., Diamond, T. H., Kumar, S., & Murrell, G. A. C. (2007). A sensitive stain for aluminum in undecalcified cancellous bone. Journal of inorganic biochemistry, 101(9), 1285-1290. Bedrick, A. E., Ramaswamy, G., & Tchertkoff, V. (1986). Histochemical determination of copper, zinc, and iron in some benign and malignant tissues. American journal of clinical pathology, 86(5), 637-640. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet Sent: Monday, 30 September 2019 20:49 To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Metals Morning all! I need some advice re: protocols to demonstrate metals in FFPE tissues. Metals such as copper, aluminum and zinc. Thanks much! Jeanine Sanders, BS, HT(ASCP), QIHC(ASCP) Centers for Diseases Control and Prevention 1600 Clifton Road NE MS H18-SB Bldg. 18, Rm SB-114 Atlanta, GA 30329 404-639-3590 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Wed Oct 2 01:49:17 2019 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 2 Oct 2019 06:49:17 +0000 Subject: [Histonet] Metals In-Reply-To: References: , <1569846163180.61@health.nsw.gov.au> Message-ID: <9c30a3ab077942dcaa4dd8655bc3ab87@SVDCMBX-MEX024.nswhealth.net> ? Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) | Principal Scientist; Adjunct Fellow, School of Medicine, University of Western Sydney; Visiting Lecturer, School of Life Sciences, Faculty of Science, University of Technology Sydney | Histopathology t: (02) 9845 3306 | e: tony.henwood at health.nsw.gov.au | w: www.schn.health.nsw.gov.au [http://res.schn.health.nsw.gov.au/signatures/facebook.gif] [http://res.schn.health.nsw.gov.au/signatures/twitter.gif] [http://res.schn.health.nsw.gov.au/signatures/chw.gif] Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia [http://res.schn.health.nsw.gov.au/signatures/shared_banner.jpg] ? Please consider the environment before printing this email. From: John Kiernan [mailto:jkiernan at uwo.ca] Sent: Wednesday, 2 October 2019 3:01 PM To: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) ; Tony Henwood (SCHN) Cc: Histonet Subject: Re: Metals Timm's sulphide-silver method is very sensitive, and modifications (mostly by Danscher) even more so. Sulphide-silver methods detect only those metals that have insoluble sulphides (copper and zinc but not aluminium, in Jeanine's list). It is necessary to fix in a special solution containing hydrogen sulphide - stink and also serious safety precautions! Tony's mention of Mallory & Parker's fresh hematoxylin stain prompted me to look it up. The 1939 paper is a free PDF download (Google Scholar: Mallory Parker Hematoxylin Stain Metals). Mallory FB, Parker F (1939) Fixing and staining methods for lead and copper in tissues. Am. J. Pathol. 15: 517-522 and Plates 83-85. The authors noted the importance of fixation (neutral formalin was OK for copper but no good for lead, which needed 95% or 100% alcohol). Like F.B. Mallory's other papers about staining methods, it's rather vague on technical details and has no references. The late Ralph D. Lillie reported more thorough investigations of staining for metals with haematoxylin in his classic book Histopathologic Technic and Practical Histochemistry (4th and last edn 1976, ISBN 0070378622), giving colours of the complexes with 30 metals introduced into tissues. I have tried Lillie's method on paraffin sections of rat tissues containing a few of these, and it works. ISBN 9781907904325 (p.333-334) may be more accessible than Lillie's book, which has become an expensive classic. For the more specific stains mentioned in Tony's message you need to do some critical reading. The best place to start may be Frieda Carson's Histotechnology textbook. ISBN 978-0891896401. Enough about metals for now! John Kiernan London, Canada = = = ________________________________ From: Tony Henwood (SCHN) via Histonet > Sent: 30 September 2019 07:22 To: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) > Cc: Histonet > Subject: Re: [Histonet] Metals Two good screening stains are Mallory and Parker?s Fresh Hematoxylin Stain for Metals and Timm?s Silver Sulphide Method for Metals. Malloy's results: Aluminium Blue-black Copper Greenish-blue Iron Blue-black Lead Blue Zinc Blue For more specific staining: Aluminon Stain for Aluminium Hydroxide Walton?s Stain for Aluminium (Phloxine binds the aluminium) Bedrick et al (1986) method for Zinc Rubeanic Acid Technique for Copper Rhodanine Technique for Copper These methods are quite sensitive but there are some specificity issues. I can provide further details and references if required. Here are some: Ohtsuki, Y., Yamaguchi, T., Sonobe, H., Takahashi, K., Hayashi, K., Takenaka, A., ... & Terao, N. (1989). Stain Technology: A Simplified Aluminum Stain in Paraffin Sections of Bone from Hemodialysis Patients. Stain technology, 64(2), 55-59. Walton, J. R., Diamond, T. H., Kumar, S., & Murrell, G. A. C. (2007). A sensitive stain for aluminum in undecalcified cancellous bone. Journal of inorganic biochemistry, 101(9), 1285-1290. Bedrick, A. E., Ramaswamy, G., & Tchertkoff, V. (1986). Histochemical determination of copper, zinc, and iron in some benign and malignant tissues. American journal of clinical pathology, 86(5), 637-640. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet > Sent: Monday, 30 September 2019 20:49 To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Metals Morning all! I need some advice re: protocols to demonstrate metals in FFPE tissues. Metals such as copper, aluminum and zinc. Thanks much! Jeanine Sanders, BS, HT(ASCP), QIHC(ASCP) Centers for Diseases Control and Prevention 1600 Clifton Road NE MS H18-SB Bldg. 18, Rm SB-114 Atlanta, GA 30329 404-639-3590 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From jqb7 at cdc.gov Wed Oct 2 06:26:50 2019 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)) Date: Wed, 2 Oct 2019 11:26:50 +0000 Subject: [Histonet] Metals In-Reply-To: References: , <1569846163180.61@health.nsw.gov.au> Message-ID: Thanks much. I have already printed Mallory & Parker's paper and I do have Frieda's book and will probably have to search for the others. My team deals with infectious diseases so this is a big learning curve for me. From: John Kiernan Sent: Wednesday, October 2, 2019 1:01 AM To: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) ; Tony Henwood (SCHN) Cc: Histonet Subject: Re: Metals Timm's sulphide-silver method is very sensitive, and modifications (mostly by Danscher) even more so. Sulphide-silver methods detect only those metals that have insoluble sulphides (copper and zinc but not aluminium, in Jeanine's list). It is necessary to fix in a special solution containing hydrogen sulphide - stink and also serious safety precautions! Tony's mention of Mallory & Parker's fresh hematoxylin stain prompted me to look it up. The 1939 paper is a free PDF download (Google Scholar: Mallory Parker Hematoxylin Stain Metals). Mallory FB, Parker F (1939) Fixing and staining methods for lead and copper in tissues. Am. J. Pathol. 15: 517-522 and Plates 83-85. The authors noted the importance of fixation (neutral formalin was OK for copper but no good for lead, which needed 95% or 100% alcohol). Like F.B. Mallory's other papers about staining methods, it's rather vague on technical details and has no references. The late Ralph D. Lillie reported more thorough investigations of staining for metals with haematoxylin in his classic book Histopathologic Technic and Practical Histochemistry (4th and last edn 1976, ISBN 0070378622), giving colours of the complexes with 30 metals introduced into tissues. I have tried Lillie's method on paraffin sections of rat tissues containing a few of these, and it works. ISBN 9781907904325 (p.333-334) may be more accessible than Lillie's book, which has become an expensive classic. For the more specific stains mentioned in Tony's message you need to do some critical reading. The best place to start may be Frieda Carson's Histotechnology textbook. ISBN 978-0891896401. Enough about metals for now! John Kiernan London, Canada = = = ________________________________ From: Tony Henwood (SCHN) via Histonet > Sent: 30 September 2019 07:22 To: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) > Cc: Histonet > Subject: Re: [Histonet] Metals Two good screening stains are Mallory and Parker's Fresh Hematoxylin Stain for Metals and Timm's Silver Sulphide Method for Metals. Malloy's results: Aluminium Blue-black Copper Greenish-blue Iron Blue-black Lead Blue Zinc Blue For more specific staining: Aluminon Stain for Aluminium Hydroxide Walton's Stain for Aluminium (Phloxine binds the aluminium) Bedrick et al (1986) method for Zinc Rubeanic Acid Technique for Copper Rhodanine Technique for Copper These methods are quite sensitive but there are some specificity issues. I can provide further details and references if required. Here are some: Ohtsuki, Y., Yamaguchi, T., Sonobe, H., Takahashi, K., Hayashi, K., Takenaka, A., ... & Terao, N. (1989). Stain Technology: A Simplified Aluminum Stain in Paraffin Sections of Bone from Hemodialysis Patients. Stain technology, 64(2), 55-59. Walton, J. R., Diamond, T. H., Kumar, S., & Murrell, G. A. C. (2007). A sensitive stain for aluminum in undecalcified cancellous bone. Journal of inorganic biochemistry, 101(9), 1285-1290. Bedrick, A. E., Ramaswamy, G., & Tchertkoff, V. (1986). Histochemical determination of copper, zinc, and iron in some benign and malignant tissues. American journal of clinical pathology, 86(5), 637-640. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP) via Histonet > Sent: Monday, 30 September 2019 20:49 To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Metals Morning all! I need some advice re: protocols to demonstrate metals in FFPE tissues. Metals such as copper, aluminum and zinc. Thanks much! Jeanine Sanders, BS, HT(ASCP), QIHC(ASCP) Centers for Diseases Control and Prevention 1600 Clifton Road NE MS H18-SB Bldg. 18, Rm SB-114 Atlanta, GA 30329 404-639-3590 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mfb.encarnacion at gmail.com Wed Oct 2 08:09:42 2019 From: mfb.encarnacion at gmail.com (Mary Faith Encarnacion) Date: Wed, 2 Oct 2019 09:09:42 -0400 Subject: [Histonet] Continuing Education Opportunities? Message-ID: Hello all, I am looking for histotech continuing education opportunities (conferences/symposiums/meetings) in the Pennsylvania area - open to traveling to New Jersey, New York, Delaware, Maryland, and Virginia. I've reached out to the region contacts listed on the NSH website, but unfortunately, I did not receive a response. Thanks for any help in advance, Mary Faith From roxjean3 at gmail.com Thu Oct 3 19:14:59 2019 From: roxjean3 at gmail.com (Roxana Robinson) Date: Thu, 3 Oct 2019 19:14:59 -0500 Subject: [Histonet] Diapath tissue processors Message-ID: <825486AF-4607-41AE-A7DF-D3B6A09D7036@gmail.com> Hello all, Has anyone heard of or is currently using this processor? I have been told it is big in Europe. Thank you Roxana Robinson From john.garratt at ciqc.ca Sun Oct 6 19:50:56 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Mon, 07 Oct 2019 00:50:56 +0000 Subject: [Histonet] =?utf-8?q?Fit-For-Purpose=C2=A0PD-L1=C2=A0Biomarker?= =?utf-8?q?=C2=A0Testing=3A_Guidelines=C2=A0For_Clinical_Laboratories?= Message-ID: Fit-For-Purpose PD-L1 Biomarker Testing For Patient Selection in Immuno-Oncology Guidelines For Clinical Laboratories From the Canadian Association of Pathologists-Association Canadienne Des Pathologistes (CAP-ACP) Click [HERE](https://journals.lww.com/appliedimmunohist/Abstract/publishahead/Fit_For_Purpose_PD_L1_Biomarker_Testing_For.98668.aspx#pdf-link) to see the abstract publish ahead of print in Applied Immunohistochemistry & Molecular Morphology: [October 03, 2019 -](https://journals.lww.com/appliedimmunohist/toc/9000/00000) LinkedIn access https://www.linkedin.com/pulse/fit-for-purpose-pd-l1-testing-guidelines-clinical-john-garratt From akemiat3377 at gmail.com Wed Oct 9 06:08:25 2019 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Wed, 9 Oct 2019 05:08:25 -0600 Subject: [Histonet] hints for cutting fatty FS Message-ID: <840C6163-E0DF-4D81-9992-7CF814AA82D5@gmail.com> A while back on histonet a few people gave some tricks to cutting fatty tissue on histonet. Someone mentioned using 50% alcohol I believe. We had some extremely fatty LN?s yesterday. The pathologist cut away most of the fat, but the tech still had a hard time, so I got called in to help the tech. I was able to get sufficient sections, but they weren?t optimal. Could any of you share your secrets please. Thank you, Akemi Allison, BS, HT/HTL (ASCP) From akemiat3377 at gmail.com Wed Oct 9 06:12:02 2019 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Wed, 9 Oct 2019 05:12:02 -0600 Subject: [Histonet] Cutting fatty FS Message-ID: Good Morning Histoland, A while back on histonet a few people gave some tricks to cutting fatty FS tissue on histonet. Someone mentioned using 50% alcohol I believe. We had some extremely fatty FS LN?s yesterday. The pathologist cut away most of the fat, but the tech still had a hard time, so I got called in to help the tech. I was able to get sufficient sections, but they weren?t optimal. Could any of you share your secrets please. Thank you, Akemi Allison, BS, HT/HTL (ASCP) From VKurth at uwhealth.org Thu Oct 10 07:17:33 2019 From: VKurth at uwhealth.org (Kurth Virginia L.) Date: Thu, 10 Oct 2019 12:17:33 +0000 Subject: [Histonet] Prostate whole mounts Message-ID: 1. As standard of care, do you process prostates as whole mounts (i.e. mega cassettes)? 2. Are there a subset of prostate resection cases that are processed in this manner (i.e. based on dx)? 3. Do you have any specimen types that are processed as whole mounts as standard of care? 4. How are these slides/blocks stored in conjunction with routine slide/block storage? 5. Are you aware of how radiologic/pathologic correlation is performed on prostate resections? From gentras at auburn.edu Thu Oct 10 12:07:51 2019 From: gentras at auburn.edu (Atoska Gentry) Date: Thu, 10 Oct 2019 17:07:51 +0000 Subject: [Histonet] Leitz Rotary Microtome Message-ID: Hello, Does anyone have experience with a Leitz Minot Rotary Microtome No. 1212? I'm particularly interested in a source for troubleshooting problem sectioning. Thanking you in advance for your assistance. Best Regards, ~Atoska From lblazek at digestivespecialists.com Thu Oct 10 12:35:26 2019 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Thu, 10 Oct 2019 17:35:26 +0000 Subject: [Histonet] HSV Message-ID: Dear all, Is there anyone out there that has a good HSV control block that they could spare? I'm sorry but I don't have anything to trade since we only have small biopsy specimens. Linda Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com From PREISZNE at mail.etsu.edu Fri Oct 11 11:10:13 2019 From: PREISZNE at mail.etsu.edu (Preiszner, Johanna) Date: Fri, 11 Oct 2019 16:10:13 +0000 Subject: [Histonet] ISO tissue with H. pylori for positive control Message-ID: Netters, could someone send us some H.pylori+ stomach to be used as positive control? I can offer Blastomyces populated human lung tissue for fungus control. We are small lab now, working for city clinics. Thanks! Hanna Preiszner From redrose297 at gmail.com Mon Oct 14 05:57:35 2019 From: redrose297 at gmail.com (warda hassan) Date: Mon, 14 Oct 2019 14:57:35 +0400 Subject: [Histonet] Bond V/S Ventna Message-ID: Hi I would request feedback on Leica-Bond and Ventna Roche as in equipment stability, Maintaince, reagents cost and results ( quality of stains) if anyone had experienced working on both platforms. Many thanks in advance for your help Good day to all From notify at thefork.com Mon Oct 14 06:08:13 2019 From: notify at thefork.com (Support TheFork) Date: Mon, 14 Oct 2019 11:08:13 +0000 (GMT) Subject: [Histonet] Request received : Bond V/S Ventna ref:_00DU0Lfqj._5001v17KBtV:ref Message-ID: We have received your request 03528422 and it is being processed by our support team. To leave additional comments, reply to this email. ref:_00DU0Lfqj._5001v17KBtV:ref From PREISZNE at mail.etsu.edu Mon Oct 14 09:00:10 2019 From: PREISZNE at mail.etsu.edu (Preiszner, Johanna) Date: Mon, 14 Oct 2019 14:00:10 +0000 Subject: [Histonet] ISO tissue with H. pylori for positive control In-Reply-To: References: Message-ID: Thank you for all the generous offers to send me control tissue! I really appreciate the help of this group! Hanna Preiszner ETSU Pathology ________________________________ From: Preiszner, Johanna Sent: Friday, October 11, 2019 12:10 PM To: histonet at lists.utsouthwestern.edu Subject: ISO tissue with H. pylori for positive control Netters, could someone send us some H.pylori+ stomach to be used as positive control? I can offer Blastomyces populated human lung tissue for fungus control. We are small lab now, working for city clinics. Thanks! Hanna Preiszner From notify at thefork.com Mon Oct 14 09:04:39 2019 From: notify at thefork.com (Support TheFork) Date: Mon, 14 Oct 2019 14:04:39 +0000 (GMT) Subject: [Histonet] Request received : Re: ISO tissue with H. pylori for positive control ref:_00DU0Lfqj._5001v17KIok:ref Message-ID: We have received your request 03529806 and it is being processed by our support team. To leave additional comments, reply to this email. ref:_00DU0Lfqj._5001v17KIok:ref From jblanchard at reliapath.com Mon Oct 14 09:46:02 2019 From: jblanchard at reliapath.com (Jeanne Blanchard) Date: Mon, 14 Oct 2019 14:46:02 +0000 Subject: [Histonet] worm contaminate Message-ID: We've been having sporadic issues of worm contaminates found in certain specimens. We've narrowed the culprit, I think at least, to be the biopsy bags (t-bags) we use for our cell blocks or to filter specimens. Has anyone else had issues like this? And/or any suggestions as to what else it could be? I've had the embedding centers cleaned, gotten a new batch of biopsy bags, and have made sure the biopsy bags are kept in their original container and/or kept closed (and the container used cleaned) - but it still happened (about a month later, but happened nonetheless). Jeanne Blanchard, CT (ASCP)CM From notify at thefork.com Mon Oct 14 09:59:13 2019 From: notify at thefork.com (Support TheFork) Date: Mon, 14 Oct 2019 14:59:13 +0000 (GMT) Subject: [Histonet] Request received : worm contaminate ref:_00DU0Lfqj._5001v17KKtw:ref Message-ID: We have received your request 03530211 and it is being processed by our support team. To leave additional comments, reply to this email. ref:_00DU0Lfqj._5001v17KKtw:ref From VKurth at uwhealth.org Tue Oct 15 09:48:40 2019 From: VKurth at uwhealth.org (Kurth Virginia L.) Date: Tue, 15 Oct 2019 14:48:40 +0000 Subject: [Histonet] ADH5 Message-ID: Does anyone know if there is a reference lab that performs ADH5. (particular the BioCare multiplex stain?) Thanks Ginny From notify at thefork.com Tue Oct 15 10:20:30 2019 From: notify at thefork.com (Support TheFork) Date: Tue, 15 Oct 2019 15:20:30 +0000 (GMT) Subject: [Histonet] Request received : ADH5 ref:_00DU0Lfqj._5001v17KmFP:ref Message-ID: We have received your request 03535723 and it is being processed by our support team. To leave additional comments, reply to this email. ref:_00DU0Lfqj._5001v17KmFP:ref From robrankin at rankinbiomed.com Tue Oct 15 15:59:33 2019 From: robrankin at rankinbiomed.com (Rob Rankin) Date: Tue, 15 Oct 2019 16:59:33 -0400 Subject: [Histonet] Prisma 6131 Special Staining Help Message-ID: Is anyone using the Prisma 6131 for special stains? GMS, FM, PAS, Congo Red, and others. We have a client in Georgia that is working on getting a Prisma 6131 ready to use for their specials and would like to speak with anyone that is currently using it for specials. Thank you!! Respectfully, *Rob Rankin, MSM, SM(ASCP)* Founder & CEO Office: 248 625-4104 ext 0012 www.rankinbiomed.com 14515 Mackey Rd. Holly, MI 48442 From subash.govender at uct.ac.za Wed Oct 16 04:28:34 2019 From: subash.govender at uct.ac.za (Subash Govender) Date: Wed, 16 Oct 2019 09:28:34 +0000 Subject: [Histonet] how to prevent bubbling in pressure cooker for immunohistochemistry procedure Message-ID: Hi there all Can anybody please tell me if they have heard of or perhaps know of a substance that can be placed in the pressure cooker during the antigen retrieval process for immunohistochemistry. This prevents excessive bubbling and lifting up of sections. I did somehow come across such info a while back, but seem to have lost it. Many thanks. Best Regards Subash Govender (UCT, Cape Town) Disclaimer - University of Cape Town This email is subject to UCT policies and email disclaimer published on our website at http://www.uct.ac.za/main/email-disclaimer or obtainable from +27 21 650 9111. If this email is not related to the business of UCT, it is sent by the sender in an individual capacity. Please report security incidents or abuse via https://csirt.uct.ac.za/page/report-an-incident.php. From mcruz84pr at gmail.com Wed Oct 16 12:43:28 2019 From: mcruz84pr at gmail.com (Maria Cruz) Date: Wed, 16 Oct 2019 13:43:28 -0400 Subject: [Histonet] How to prevent bubbling in pressure cooker for immunohistochemistry procedure Message-ID: Subash: Since the main purpose of using a pressure cooker for HIER is the rapid and reliable pre-treatment of sections while PREVENTING boiling and potential tissue detachment, you really shouldn?t be experiencing any bubbling. If you are, then your PC isn?t sealed adequately and the only way to fix that problem is to replace the gasket or find another way to tighten the lid.. I?ve never heard of anything that you can put inside of a PC that can change the way it pressurizes, so I?ll be following this thread to see if anyone else can contribute. Maria ************************************** Hi there all Can anybody please tell me if they have heard of or perhaps know of a substance that can be placed in the pressure cooker during the antigen retrieval process for immunohistochemistry. This prevents excessive bubbling and lifting up of sections. I did somehow come across such info a while back, but seem to have lost it. Many thanks. Best Regards Subash Govender From sprice2003 at gmail.com Wed Oct 16 14:06:02 2019 From: sprice2003 at gmail.com (Sally Price) Date: Wed, 16 Oct 2019 15:06:02 -0400 Subject: [Histonet] ADH5 multiplex procedure Message-ID: ---------- Forwarded message ---------- From: "Kurth Virginia L." To: "histonet at lists.utsouthwestern.edu" Virginia: I?m pretty certain that the ADH5 multiplex procedure is performed at CSI Labs in the Atlanta area. And I don?t think that biocare refers to it by that name any more either - I think they just spell out all the antigens that the antibodies in this cocktail can identify, like CK-5/14+p63+CK-7/18. Sally - - - - - - - - - - - Date: Tue, 15 Oct 2019 14:48:40 Subject: [Histonet] ADH5 Does anyone know if there is a reference lab that performs ADH5. (particular the BioCare multiplex stain?) Thanks Ginny-- Sally Price From gentras at auburn.edu Thu Oct 17 10:16:58 2019 From: gentras at auburn.edu (Atoska Gentry) Date: Thu, 17 Oct 2019 15:16:58 +0000 Subject: [Histonet] cleaning old charged slides Message-ID: Hello, Has anyone had success cleaning old charged slides which have become foggy over time. Thanks! ~Atoska From c.tague at pathologyarts.com Thu Oct 17 11:00:18 2019 From: c.tague at pathologyarts.com (Curt Tague) Date: Thu, 17 Oct 2019 16:00:18 +0000 Subject: [Histonet] procuring fresh tumor tissue Message-ID: Hello again histonet-ers- I am working with a company do validate a new rapid (another new) tissue processor... we are currently in the process of submitting a proof of concept paper to CAP then will submit a formal white paper with data. What I am curious about is locating tumor tissue that can be used to parallel this study, obviously one part processed with the standard process we are all accustomed to and the other piece processed with this new technology. We are running all the necessary tests to show consistency and comparable results, H&E, special stains, IHC, DNA and RNA extraction... what I could use a little help with is sourcing some tumor that has not been in formalin for weeks and weeks... specifically breast tumor and possibly lung tumor to demonstrate all the downstream tests are not compromised with this technology. Is there any source out there someone could recommend and/or, if you are in a large hospital setting, is that something you might be able to assist with, when a tumor is identified and not entirely needed for diagnostics? All the best to you all, thanks for your thoughts, Curt From dave.c.juliano at gmail.com Thu Oct 17 11:21:09 2019 From: dave.c.juliano at gmail.com (Dave Juliano) Date: Thu, 17 Oct 2019 09:21:09 -0700 Subject: [Histonet] cleaning old charged slides In-Reply-To: References: Message-ID: Hi Atsoka! I?d be willing to trade you fresh slides for those foggy ones- studying effects of age on charges slides. Contact me at: Dave.c.juliano at gmail dot com if you?re interested! On Thu, Oct 17, 2019 at 8:24 AM Atoska Gentry via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello, > > Has anyone had success cleaning old charged slides which have become foggy > over time. > > Thanks! > ~Atoska > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 at earthlink.net Thu Oct 17 11:27:50 2019 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 17 Oct 2019 12:27:50 -0400 Subject: [Histonet] ICYMI here is a link to part 3 of 3 in my series on Relocation - Relocation an Action Plan. And our latest histology opportunties on and off the bench! Message-ID: <014d01d58507$d24bdcb0$76e39610$@earthlink.net> Hi Histonetters, ICYMI - In case you missed it!! I am so excited!! My latest article has been published in the NSH Quarterly Career Center Newsletter under my byline: Make the Cut My latest article is entitled: Relocation ? An Action Plan This is part 3 of 3 in my series on relocation. You can find a link to parts 1&2 in this article as well! This is the link to my article on the NSH?s Fixation on Histology Blog and it is also in the current edition of the NSH Career Newsletter. Here is the link to the article: https://www.fixationonhistology.com/post/relocation-part-3-action-plan If you have a minute to read it I would love to hear what you think. If you can?t get to the article with this link let me know and I will send you a copy of it. Histopeeps, we also have amazing opportunities nationwide! Leadership and Specialty opportunities: 1. Chicago Greater Chicago area-Field Applications Specialist ? IHC 2. Boston Greater Boston area-Field Applications Specialist ? IHC 3. Los Angeles Manager of Customer Training and Support 4. Nashville Histology Supervisor 5. Kalamazoo Applications Specialist 6. Orlando Histology Instructor HT or HTL or elig opportunities: 1. California Northern CA IHC Specialist 2. California Northern CA Histology tech 3. N. Carolina Raleigh/Durham area Histology Tech 4. Colorado Denver area ? IHC Histology Tech 5. Wisconsin Milwaukee ? Histology Tech 6. Georgia Columbus ? Histotech dermpath 7. S. Carolina Beaufort ? Histology Tech Please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral reward! My clients offer competitive pay rates, excellent benefits and in most cases Relocation assistance or a sign on bonus. They are ready to interview and hire!!! If You or Anyone You Know Might Be Interested In a New Opportunity, Please Contact Me ASAP call or text me on my cell At 407-353-5070. If you want some additional information or to set up a time to chat please call me toll free at 866-607-3542 or email me at relia1 at earthlink.net Have a great day. I look forward to hearing back from you. Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From relia1 at earthlink.net Thu Oct 17 13:23:52 2019 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 17 Oct 2019 14:23:52 -0400 Subject: [Histonet] RELIA HOT JOB ALERT! IHC Specialist for New England area. Contact me for info ASAP! Message-ID: <01ce01d58518$0759e9a0$160dbce0$@earthlink.net> Hi Histonetters! I hope you are having a great day and cruising into the weekend. I have an opportunity that I want to share in hopes that you or someone you know might be interested. I am looking for a Field Applications Support Specialist ?IHC for a territory in New England. This is a RELIA EXCLUSIVE! My client offers an excellent product line and reputation to support it. This is a full time salaried position NO sales required. Use your IHC expertise to help my client?s customers further their support of patient care. My client is offering a very competitive salary, travel expenses, benefits and more. If you would like details on the opportunity please contact me ASAP! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From M.Donovan at alfred.org.au Thu Oct 17 18:10:56 2019 From: M.Donovan at alfred.org.au (Donovan, Mark) Date: Thu, 17 Oct 2019 23:10:56 +0000 Subject: [Histonet] How to prevent bubbling in pressure cooker for immunohistochemistry procedure Message-ID: Sabash, What I think you may be referring to is the use of a surfactant in the antigen retrieval solution to lower the surface tension. The theory being it reduces bubble formation in the retrieval solution at high temperature and hence disruption of tissue. Before moving to commercial antigen retrieval solutions we would add Tween 20 to a concentration of 0.05% to our in house citrate or EDTA retrieval solutions to achieve this effect. Regards, Mark Original message: Date: Wed, 16 Oct 2019 13:43:28 -0400 From: Maria Cruz > To: "histonet at lists.utsouthwestern.edu" > Subject: Re: [Histonet] How to prevent bubbling in pressure cooker for immunohistochemistry procedure Message-ID: > Content-Type: text/plain; charset="UTF-8" Subash: Since the main purpose of using a pressure cooker for HIER is the rapid and reliable pre-treatment of sections while PREVENTING boiling and potential tissue detachment, you really shouldn?t be experiencing any bubbling. If you are, then your PC isn?t sealed adequately and the only way to fix that problem is to replace the gasket or find another way to tighten the lid.. I?ve never heard of anything that you can put inside of a PC that can change the way it pressurizes, so I?ll be following this thread to see if anyone else can contribute. Maria ************************************** Hi there all Can anybody please tell me if they have heard of or perhaps know of a substance that can be placed in the pressure cooker during the antigen retrieval process for immunohistochemistry. This prevents excessive bubbling and lifting up of sections. I did somehow come across such info a while back, but seem to have lost it. Many thanks. Best Regards Subash Govender From carl.hobbs at kcl.ac.uk Fri Oct 18 12:58:12 2019 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 18 Oct 2019 17:58:12 +0000 Subject: [Histonet] How to prevent bubbling in pressure cooker for immunohistochemistry procedure Message-ID: Hi Subash Imho your sections are not sufficiently adherent to the slide(s) It is not M/W "bubbling" that causes your problem If you use Superfrost Plus/lab-coated Silane or even double subbed slides ( thanks Ole!) AND dry your sections onto the slides efficiently, you will not get section-loss. NB: SF+ are expensive: if you cannot afford them....experiment with in-house Silane ( APES) or subbing, to coat your IHC slides. If you use the latter methods, make sure you slides are very clean. Unless the tissue is very fibrous/bone/cartilage/severely inflamed....difficult to keep sections adherent. In the latter case, Trajan 3 series can help much more than std coated slides. What tissues are causing you problems? For most soft tissues I use Superfrost Plus slides, dry the mounted sections under a fan O/N ( at least one hour) For my peace of mind I then put the slides into a 60C oven for 30-60 mins before dewaxing. Please post your opinion/results so far? Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 From subash.govender at uct.ac.za Fri Oct 18 18:00:16 2019 From: subash.govender at uct.ac.za (Subash Govender) Date: Fri, 18 Oct 2019 23:00:16 +0000 Subject: [Histonet] Histonet Digest, Vol 191, Issue 10 In-Reply-To: References: Message-ID: Re: How to prevent bubbling in pressure cooker for immunohistochemistry procedure. Maria Thank you so much for your feedback. The instrument i use for the antigen retrieval process is just household 6L Russek Hobbs pressure cooker. Before i place my slides into this PC i do actually wait for it to boil and actually do see the solution bubble. Then only do i put my slides in and close the lid. I am assuming it continues to bubble while reaching its optimum temperature, but you think not, so i am unsure. This procedure works quite well as we get good results. However, i do get an odd 1 or 2 tissues washing off now and again. So to try and stop this completely and to perfect our method i thought about this article i read, but sadly have lost. As i do think that maybe no bubbling will prevent this. But could be wrong. Will keep searching for the info i lost. Thanks again, keep well. Best Regards Subash Get Outlook for Android ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Thursday, October 17, 2019 7:00:01 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 191, Issue 10 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: How to prevent bubbling in pressure cooker for immunohistochemistry procedure (Maria Cruz) 2. Re: ADH5 multiplex procedure (Sally Price) 3. Re: cleaning old charged slides (Atoska Gentry) 4. procuring fresh tumor tissue (Curt Tague) 5. Re: cleaning old charged slides (Dave Juliano) 6. ICYMI here is a link to part 3 of 3 in my series on Relocation - Relocation an Action Plan. And our latest histology opportunties on and off the bench! (Pam Barker) ---------------------------------------------------------------------- Message: 1 Date: Wed, 16 Oct 2019 13:43:28 -0400 From: Maria Cruz To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] How to prevent bubbling in pressure cooker for immunohistochemistry procedure Message-ID: Content-Type: text/plain; charset="UTF-8" Subash: Since the main purpose of using a pressure cooker for HIER is the rapid and reliable pre-treatment of sections while PREVENTING boiling and potential tissue detachment, you really shouldn?t be experiencing any bubbling. If you are, then your PC isn?t sealed adequately and the only way to fix that problem is to replace the gasket or find another way to tighten the lid.. I?ve never heard of anything that you can put inside of a PC that can change the way it pressurizes, so I?ll be following this thread to see if anyone else can contribute. Maria ************************************** Hi there all Can anybody please tell me if they have heard of or perhaps know of a substance that can be placed in the pressure cooker during the antigen retrieval process for immunohistochemistry. This prevents excessive bubbling and lifting up of sections. I did somehow come across such info a while back, but seem to have lost it. Many thanks. Best Regards Subash Govender ------------------------------ Message: 2 Date: Wed, 16 Oct 2019 15:06:02 -0400 From: Sally Price To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] ADH5 multiplex procedure Message-ID: Content-Type: text/plain; charset="UTF-8" ---------- Forwarded message ---------- From: "Kurth Virginia L." To: "histonet at lists.utsouthwestern.edu" Virginia: I?m pretty certain that the ADH5 multiplex procedure is performed at CSI Labs in the Atlanta area. And I don?t think that biocare refers to it by that name any more either - I think they just spell out all the antigens that the antibodies in this cocktail can identify, like CK-5/14+p63+CK-7/18. Sally - - - - - - - - - - - Date: Tue, 15 Oct 2019 14:48:40 Subject: [Histonet] ADH5 Does anyone know if there is a reference lab that performs ADH5. (particular the BioCare multiplex stain?) Thanks Ginny-- Sally Price ------------------------------ Message: 3 Date: Thu, 17 Oct 2019 15:16:58 +0000 From: Atoska Gentry To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] cleaning old charged slides Message-ID: Content-Type: text/plain; charset="us-ascii" Hello, Has anyone had success cleaning old charged slides which have become foggy over time. Thanks! ~Atoska ------------------------------ Message: 4 Date: Thu, 17 Oct 2019 16:00:18 +0000 From: Curt Tague To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] procuring fresh tumor tissue Message-ID: Content-Type: text/plain; charset="us-ascii" Hello again histonet-ers- I am working with a company do validate a new rapid (another new) tissue processor... we are currently in the process of submitting a proof of concept paper to CAP then will submit a formal white paper with data. What I am curious about is locating tumor tissue that can be used to parallel this study, obviously one part processed with the standard process we are all accustomed to and the other piece processed with this new technology. We are running all the necessary tests to show consistency and comparable results, H&E, special stains, IHC, DNA and RNA extraction... what I could use a little help with is sourcing some tumor that has not been in formalin for weeks and weeks... specifically breast tumor and possibly lung tumor to demonstrate all the downstream tests are not compromised with this technology. Is there any source out there someone could recommend and/or, if you are in a large hospital setting, is that something you might be able to assist with, when a tumor is identified and not entirely needed for diagnostics? All the best to you all, thanks for your thoughts, Curt ------------------------------ Message: 5 Date: Thu, 17 Oct 2019 09:21:09 -0700 From: Dave Juliano To: Atoska Gentry Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] cleaning old charged slides Message-ID: Content-Type: text/plain; charset="UTF-8" Hi Atsoka! I?d be willing to trade you fresh slides for those foggy ones- studying effects of age on charges slides. Contact me at: Dave.c.juliano at gmail dot com if you?re interested! On Thu, Oct 17, 2019 at 8:24 AM Atoska Gentry via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello, > > Has anyone had success cleaning old charged slides which have become foggy > over time. > > Thanks! > ~Atoska > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 6 Date: Thu, 17 Oct 2019 12:27:50 -0400 From: "Pam Barker" To: "Histopeeps Histonet" Subject: [Histonet] ICYMI here is a link to part 3 of 3 in my series on Relocation - Relocation an Action Plan. And our latest histology opportunties on and off the bench! Message-ID: <014d01d58507$d24bdcb0$76e39610$@earthlink.net> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters, ICYMI - In case you missed it!! I am so excited!! My latest article has been published in the NSH Quarterly Career Center Newsletter under my byline: Make the Cut My latest article is entitled: Relocation ? An Action Plan This is part 3 of 3 in my series on relocation. You can find a link to parts 1&2 in this article as well! This is the link to my article on the NSH?s Fixation on Histology Blog and it is also in the current edition of the NSH Career Newsletter. Here is the link to the article: https://www.fixationonhistology.com/post/relocation-part-3-action-plan If you have a minute to read it I would love to hear what you think. If you can?t get to the article with this link let me know and I will send you a copy of it. Histopeeps, we also have amazing opportunities nationwide! Leadership and Specialty opportunities: 1. Chicago Greater Chicago area-Field Applications Specialist ? IHC 2. Boston Greater Boston area-Field Applications Specialist ? IHC 3. Los Angeles Manager of Customer Training and Support 4. Nashville Histology Supervisor 5. Kalamazoo Applications Specialist 6. Orlando Histology Instructor HT or HTL or elig opportunities: 1. California Northern CA IHC Specialist 2. California Northern CA Histology tech 3. N. Carolina Raleigh/Durham area Histology Tech 4. Colorado Denver area ? IHC Histology Tech 5. Wisconsin Milwaukee ? Histology Tech 6. Georgia Columbus ? Histotech dermpath 7. S. Carolina Beaufort ? Histology Tech Please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral reward! My clients offer competitive pay rates, excellent benefits and in most cases Relocation assistance or a sign on bonus. They are ready to interview and hire!!! If You or Anyone You Know Might Be Interested In a New Opportunity, Please Contact Me ASAP call or text me on my cell At 407-353-5070. If you want some additional information or to set up a time to chat please call me toll free at 866-607-3542 or email me at relia1 at earthlink.net Have a great day. I look forward to hearing back from you. Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 191, Issue 10 ***************************************** Disclaimer - University of Cape Town This email is subject to UCT policies and email disclaimer published on our website at http://www.uct.ac.za/main/email-disclaimer or obtainable from +27 21 650 9111. If this email is not related to the business of UCT, it is sent by the sender in an individual capacity. Please report security incidents or abuse via https://csirt.uct.ac.za/page/report-an-incident.php. From subash.govender at uct.ac.za Fri Oct 18 18:16:41 2019 From: subash.govender at uct.ac.za (Subash Govender) Date: Fri, 18 Oct 2019 23:16:41 +0000 Subject: [Histonet] Histonet Digest, Vol 191, Issue 11 In-Reply-To: References: Message-ID: RE:How to prevent bubbling in pressure cooker for immunohistochemistry procedure. Hi there Mark. Really appreciate your feedback. I do use tween 20 in the retrieval solution but actually thought it was to clean the slides, thus preventing background staining. I did not know it prevents bubble formation, so i have learnt something. However, i use it at a 0.5 % solution. Maybe thats too much as the solution looks a bit soapy, and could also cause lifting of sections im presuming. Thanks again, keep well. Best regards Subash Get Outlook for Android ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Friday, October 18, 2019 7:00:01 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 191, Issue 11 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RELIA HOT JOB ALERT! IHC Specialist for New England area. Contact me for info ASAP! (Pam Barker) 2. How to prevent bubbling in pressure cooker for immunohistochemistry procedure (Donovan, Mark) ---------------------------------------------------------------------- Message: 1 Date: Thu, 17 Oct 2019 14:23:52 -0400 From: "Pam Barker" To: "Histopeeps Histonet" Subject: [Histonet] RELIA HOT JOB ALERT! IHC Specialist for New England area. Contact me for info ASAP! Message-ID: <01ce01d58518$0759e9a0$160dbce0$@earthlink.net> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters! I hope you are having a great day and cruising into the weekend. I have an opportunity that I want to share in hopes that you or someone you know might be interested. I am looking for a Field Applications Support Specialist ?IHC for a territory in New England. This is a RELIA EXCLUSIVE! My client offers an excellent product line and reputation to support it. This is a full time salaried position NO sales required. Use your IHC expertise to help my client?s customers further their support of patient care. My client is offering a very competitive salary, travel expenses, benefits and more. If you would like details on the opportunity please contact me ASAP! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 2 Date: Thu, 17 Oct 2019 23:10:56 +0000 From: "Donovan, Mark" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] How to prevent bubbling in pressure cooker for immunohistochemistry procedure Message-ID: Content-Type: text/plain; charset="us-ascii" Sabash, What I think you may be referring to is the use of a surfactant in the antigen retrieval solution to lower the surface tension. The theory being it reduces bubble formation in the retrieval solution at high temperature and hence disruption of tissue. Before moving to commercial antigen retrieval solutions we would add Tween 20 to a concentration of 0.05% to our in house citrate or EDTA retrieval solutions to achieve this effect. Regards, Mark Original message: Date: Wed, 16 Oct 2019 13:43:28 -0400 From: Maria Cruz > To: "histonet at lists.utsouthwestern.edu" > Subject: Re: [Histonet] How to prevent bubbling in pressure cooker for immunohistochemistry procedure Message-ID: > Content-Type: text/plain; charset="UTF-8" Subash: Since the main purpose of using a pressure cooker for HIER is the rapid and reliable pre-treatment of sections while PREVENTING boiling and potential tissue detachment, you really shouldn?t be experiencing any bubbling. If you are, then your PC isn?t sealed adequately and the only way to fix that problem is to replace the gasket or find another way to tighten the lid.. I?ve never heard of anything that you can put inside of a PC that can change the way it pressurizes, so I?ll be following this thread to see if anyone else can contribute. Maria ************************************** Hi there all Can anybody please tell me if they have heard of or perhaps know of a substance that can be placed in the pressure cooker during the antigen retrieval process for immunohistochemistry. This prevents excessive bubbling and lifting up of sections. I did somehow come across such info a while back, but seem to have lost it. Many thanks. Best Regards Subash Govender ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 191, Issue 11 ***************************************** Disclaimer - University of Cape Town This email is subject to UCT policies and email disclaimer published on our website at http://www.uct.ac.za/main/email-disclaimer or obtainable from +27 21 650 9111. If this email is not related to the business of UCT, it is sent by the sender in an individual capacity. Please report security incidents or abuse via https://csirt.uct.ac.za/page/report-an-incident.php. From subash.govender at uct.ac.za Sun Oct 20 11:30:46 2019 From: subash.govender at uct.ac.za (Subash Govender) Date: Sun, 20 Oct 2019 16:30:46 +0000 Subject: [Histonet] Histonet Digest, Vol 191, Issue 12 In-Reply-To: References: Message-ID: Re: How to prevent bubbling in pressure cooker for the immunohistichemistry procedure Hi there Carl Thank you so much for your advice. I do actually use Marienfeld Histobond slides, they are silane coated. So dont think that is the prolem, but i do like your advice of drying the tissue under the fan. The tissues that give me problems are breast, cervix, and other odd tissues. With breast tissue being fatty i suppose you would have a bit of lifting. Another kind lady also gave me the idea of actually placing the slides into the pressure cooker and sealing the lid immediately after being dewaxed instead of waiting for the solution to boil first like i do. Thanks again for your feedback Carl, much appreciated. Subash Get Outlook for Android ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Saturday, October 19, 2019 7:00:01 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 191, Issue 12 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: How to prevent bubbling in pressure cooker for immunohistochemistry procedure (Hobbs, Carl) 2. Re: Histonet Digest, Vol 191, Issue 10 (Subash Govender) 3. Re: Histonet Digest, Vol 191, Issue 11 (Subash Govender) ---------------------------------------------------------------------- Message: 1 Date: Fri, 18 Oct 2019 17:58:12 +0000 From: "Hobbs, Carl" To: histonet Subject: Re: [Histonet] How to prevent bubbling in pressure cooker for immunohistochemistry procedure Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Subash Imho your sections are not sufficiently adherent to the slide(s) It is not M/W "bubbling" that causes your problem If you use Superfrost Plus/lab-coated Silane or even double subbed slides ( thanks Ole!) AND dry your sections onto the slides efficiently, you will not get section-loss. NB: SF+ are expensive: if you cannot afford them....experiment with in-house Silane ( APES) or subbing, to coat your IHC slides. If you use the latter methods, make sure you slides are very clean. Unless the tissue is very fibrous/bone/cartilage/severely inflamed....difficult to keep sections adherent. In the latter case, Trajan 3 series can help much more than std coated slides. What tissues are causing you problems? For most soft tissues I use Superfrost Plus slides, dry the mounted sections under a fan O/N ( at least one hour) For my peace of mind I then put the slides into a 60C oven for 30-60 mins before dewaxing. Please post your opinion/results so far? Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ------------------------------ Message: 2 Date: Fri, 18 Oct 2019 23:00:16 +0000 From: Subash Govender To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Histonet Digest, Vol 191, Issue 10 Message-ID: Content-Type: text/plain; charset="Windows-1252" Re: How to prevent bubbling in pressure cooker for immunohistochemistry procedure. Maria Thank you so much for your feedback. The instrument i use for the antigen retrieval process is just household 6L Russek Hobbs pressure cooker. Before i place my slides into this PC i do actually wait for it to boil and actually do see the solution bubble. Then only do i put my slides in and close the lid. I am assuming it continues to bubble while reaching its optimum temperature, but you think not, so i am unsure. This procedure works quite well as we get good results. However, i do get an odd 1 or 2 tissues washing off now and again. So to try and stop this completely and to perfect our method i thought about this article i read, but sadly have lost. As i do think that maybe no bubbling will prevent this. But could be wrong. Will keep searching for the info i lost. Thanks again, keep well. Best Regards Subash Get Outlook for Android> ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Thursday, October 17, 2019 7:00:01 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 191, Issue 10 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet> or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: How to prevent bubbling in pressure cooker for immunohistochemistry procedure (Maria Cruz) 2. Re: ADH5 multiplex procedure (Sally Price) 3. Re: cleaning old charged slides (Atoska Gentry) 4. procuring fresh tumor tissue (Curt Tague) 5. Re: cleaning old charged slides (Dave Juliano) 6. ICYMI here is a link to part 3 of 3 in my series on Relocation - Relocation an Action Plan. And our latest histology opportunties on and off the bench! (Pam Barker) ---------------------------------------------------------------------- Message: 1 Date: Wed, 16 Oct 2019 13:43:28 -0400 From: Maria Cruz To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] How to prevent bubbling in pressure cooker for immunohistochemistry procedure Message-ID: Content-Type: text/plain; charset="UTF-8" Subash: Since the main purpose of using a pressure cooker for HIER is the rapid and reliable pre-treatment of sections while PREVENTING boiling and potential tissue detachment, you really shouldn?t be experiencing any bubbling. If you are, then your PC isn?t sealed adequately and the only way to fix that problem is to replace the gasket or find another way to tighten the lid.. I?ve never heard of anything that you can put inside of a PC that can change the way it pressurizes, so I?ll be following this thread to see if anyone else can contribute. Maria ************************************** Hi there all Can anybody please tell me if they have heard of or perhaps know of a substance that can be placed in the pressure cooker during the antigen retrieval process for immunohistochemistry. This prevents excessive bubbling and lifting up of sections. I did somehow come across such info a while back, but seem to have lost it. Many thanks. Best Regards Subash Govender ------------------------------ Message: 2 Date: Wed, 16 Oct 2019 15:06:02 -0400 From: Sally Price To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] ADH5 multiplex procedure Message-ID: Content-Type: text/plain; charset="UTF-8" ---------- Forwarded message ---------- From: "Kurth Virginia L." To: "histonet at lists.utsouthwestern.edu" Virginia: I?m pretty certain that the ADH5 multiplex procedure is performed at CSI Labs in the Atlanta area. And I don?t think that biocare refers to it by that name any more either - I think they just spell out all the antigens that the antibodies in this cocktail can identify, like CK-5/14+p63+CK-7/18. Sally - - - - - - - - - - - Date: Tue, 15 Oct 2019 14:48:40 Subject: [Histonet] ADH5 Does anyone know if there is a reference lab that performs ADH5. (particular the BioCare multiplex stain?) Thanks Ginny-- Sally Price ------------------------------ Message: 3 Date: Thu, 17 Oct 2019 15:16:58 +0000 From: Atoska Gentry To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] cleaning old charged slides Message-ID: Content-Type: text/plain; charset="us-ascii" Hello, Has anyone had success cleaning old charged slides which have become foggy over time. Thanks! ~Atoska ------------------------------ Message: 4 Date: Thu, 17 Oct 2019 16:00:18 +0000 From: Curt Tague To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] procuring fresh tumor tissue Message-ID: Content-Type: text/plain; charset="us-ascii" Hello again histonet-ers- I am working with a company do validate a new rapid (another new) tissue processor... we are currently in the process of submitting a proof of concept paper to CAP then will submit a formal white paper with data. What I am curious about is locating tumor tissue that can be used to parallel this study, obviously one part processed with the standard process we are all accustomed to and the other piece processed with this new technology. We are running all the necessary tests to show consistency and comparable results, H&E, special stains, IHC, DNA and RNA extraction... what I could use a little help with is sourcing some tumor that has not been in formalin for weeks and weeks... specifically breast tumor and possibly lung tumor to demonstrate all the downstream tests are not compromised with this technology. Is there any source out there someone could recommend and/or, if you are in a large hospital setting, is that something you might be able to assist with, when a tumor is identified and not entirely needed for diagnostics? All the best to you all, thanks for your thoughts, Curt ------------------------------ Message: 5 Date: Thu, 17 Oct 2019 09:21:09 -0700 From: Dave Juliano To: Atoska Gentry Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] cleaning old charged slides Message-ID: Content-Type: text/plain; charset="UTF-8" Hi Atsoka! I?d be willing to trade you fresh slides for those foggy ones- studying effects of age on charges slides. Contact me at: Dave.c.juliano at gmail dot com if you?re interested! On Thu, Oct 17, 2019 at 8:24 AM Atoska Gentry via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello, > > Has anyone had success cleaning old charged slides which have become foggy > over time. > > Thanks! > ~Atoska > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > ------------------------------ Message: 6 Date: Thu, 17 Oct 2019 12:27:50 -0400 From: "Pam Barker" To: "Histopeeps Histonet" Subject: [Histonet] ICYMI here is a link to part 3 of 3 in my series on Relocation - Relocation an Action Plan. And our latest histology opportunties on and off the bench! Message-ID: <014d01d58507$d24bdcb0$76e39610$@earthlink.net> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters, ICYMI - In case you missed it!! I am so excited!! My latest article has been published in the NSH Quarterly Career Center Newsletter under my byline: Make the Cut My latest article is entitled: Relocation ? An Action Plan This is part 3 of 3 in my series on relocation. You can find a link to parts 1&2 in this article as well! This is the link to my article on the NSH?s Fixation on Histology Blog and it is also in the current edition of the NSH Career Newsletter. Here is the link to the article: https://www.fixationonhistology.com/post/relocation-part-3-action-plan> If you have a minute to read it I would love to hear what you think. If you can?t get to the article with this link let me know and I will send you a copy of it. Histopeeps, we also have amazing opportunities nationwide! Leadership and Specialty opportunities: 1. Chicago Greater Chicago area-Field Applications Specialist ? IHC 2. Boston Greater Boston area-Field Applications Specialist ? IHC 3. Los Angeles Manager of Customer Training and Support 4. Nashville Histology Supervisor 5. Kalamazoo Applications Specialist 6. Orlando Histology Instructor HT or HTL or elig opportunities: 1. California Northern CA IHC Specialist 2. California Northern CA Histology tech 3. N. Carolina Raleigh/Durham area Histology Tech 4. Colorado Denver area ? IHC Histology Tech 5. Wisconsin Milwaukee ? Histology Tech 6. Georgia Columbus ? Histotech dermpath 7. S. Carolina Beaufort ? Histology Tech Please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral reward! My clients offer competitive pay rates, excellent benefits and in most cases Relocation assistance or a sign on bonus. They are ready to interview and hire!!! If You or Anyone You Know Might Be Interested In a New Opportunity, Please Contact Me ASAP call or text me on my cell At 407-353-5070. If you want some additional information or to set up a time to chat please call me toll free at 866-607-3542 or email me at relia1 at earthlink.net Have a great day. I look forward to hearing back from you. Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA> www.linkedin.com/in/reliasolutions> www.twitter.com/pamatrelia> ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet> ------------------------------ End of Histonet Digest, Vol 191, Issue 10 ***************************************** Disclaimer - University of Cape Town This email is subject to UCT policies and email disclaimer published on our website at http://www.uct.ac.za/main/email-disclaimer or obtainable from +27 21 650 9111. If this email is not related to the business of UCT, it is sent by the sender in an individual capacity. Please report security incidents or abuse via https://csirt.uct.ac.za/page/report-an-incident.php. ------------------------------ Message: 3 Date: Fri, 18 Oct 2019 23:16:41 +0000 From: Subash Govender To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Histonet Digest, Vol 191, Issue 11 Message-ID: Content-Type: text/plain; charset="Windows-1252" RE:How to prevent bubbling in pressure cooker for immunohistochemistry procedure. Hi there Mark. Really appreciate your feedback. I do use tween 20 in the retrieval solution but actually thought it was to clean the slides, thus preventing background staining. I did not know it prevents bubble formation, so i have learnt something. However, i use it at a 0.5 % solution. Maybe thats too much as the solution looks a bit soapy, and could also cause lifting of sections im presuming. Thanks again, keep well. Best regards Subash Get Outlook for Android> ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Friday, October 18, 2019 7:00:01 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 191, Issue 11 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet> or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RELIA HOT JOB ALERT! IHC Specialist for New England area. Contact me for info ASAP! (Pam Barker) 2. How to prevent bubbling in pressure cooker for immunohistochemistry procedure (Donovan, Mark) ---------------------------------------------------------------------- Message: 1 Date: Thu, 17 Oct 2019 14:23:52 -0400 From: "Pam Barker" To: "Histopeeps Histonet" Subject: [Histonet] RELIA HOT JOB ALERT! IHC Specialist for New England area. Contact me for info ASAP! Message-ID: <01ce01d58518$0759e9a0$160dbce0$@earthlink.net> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters! I hope you are having a great day and cruising into the weekend. I have an opportunity that I want to share in hopes that you or someone you know might be interested. I am looking for a Field Applications Support Specialist ?IHC for a territory in New England. This is a RELIA EXCLUSIVE! My client offers an excellent product line and reputation to support it. This is a full time salaried position NO sales required. Use your IHC expertise to help my client?s customers further their support of patient care. My client is offering a very competitive salary, travel expenses, benefits and more. If you would like details on the opportunity please contact me ASAP! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA> www.linkedin.com/in/reliasolutions> www.twitter.com/pamatrelia> ------------------------------ Message: 2 Date: Thu, 17 Oct 2019 23:10:56 +0000 From: "Donovan, Mark" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] How to prevent bubbling in pressure cooker for immunohistochemistry procedure Message-ID: Content-Type: text/plain; charset="us-ascii" Sabash, What I think you may be referring to is the use of a surfactant in the antigen retrieval solution to lower the surface tension. The theory being it reduces bubble formation in the retrieval solution at high temperature and hence disruption of tissue. Before moving to commercial antigen retrieval solutions we would add Tween 20 to a concentration of 0.05% to our in house citrate or EDTA retrieval solutions to achieve this effect. Regards, Mark Original message: Date: Wed, 16 Oct 2019 13:43:28 -0400 From: Maria Cruz > To: "histonet at lists.utsouthwestern.edu" > Subject: Re: [Histonet] How to prevent bubbling in pressure cooker for immunohistochemistry procedure Message-ID: > Content-Type: text/plain; charset="UTF-8" Subash: Since the main purpose of using a pressure cooker for HIER is the rapid and reliable pre-treatment of sections while PREVENTING boiling and potential tissue detachment, you really shouldn?t be experiencing any bubbling. If you are, then your PC isn?t sealed adequately and the only way to fix that problem is to replace the gasket or find another way to tighten the lid.. I?ve never heard of anything that you can put inside of a PC that can change the way it pressurizes, so I?ll be following this thread to see if anyone else can contribute. Maria ************************************** Hi there all Can anybody please tell me if they have heard of or perhaps know of a substance that can be placed in the pressure cooker during the antigen retrieval process for immunohistochemistry. This prevents excessive bubbling and lifting up of sections. I did somehow come across such info a while back, but seem to have lost it. Many thanks. Best Regards Subash Govender ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet> ------------------------------ End of Histonet Digest, Vol 191, Issue 11 ***************************************** Disclaimer - University of Cape Town This email is subject to UCT policies and email disclaimer published on our website at http://www.uct.ac.za/main/email-disclaimer or obtainable from +27 21 650 9111. If this email is not related to the business of UCT, it is sent by the sender in an individual capacity. Please report security incidents or abuse via https://csirt.uct.ac.za/page/report-an-incident.php. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 191, Issue 12 ***************************************** Disclaimer - University of Cape Town This email is subject to UCT policies and email disclaimer published on our website at http://www.uct.ac.za/main/email-disclaimer or obtainable from +27 21 650 9111. If this email is not related to the business of UCT, it is sent by the sender in an individual capacity. Please report security incidents or abuse via https://csirt.uct.ac.za/page/report-an-incident.php. From Dawn.Olszewski at SGMC.ORG Mon Oct 21 15:19:48 2019 From: Dawn.Olszewski at SGMC.ORG (Olszewski, Dawn) Date: Mon, 21 Oct 2019 20:19:48 +0000 Subject: [Histonet] Extremity carrier References: <12a3486f-aba0-466f-9fef-6a4ca11e0a30.3b5c5640-2195-4f35-b5ef-5e1ebee68512.5202a337-b4de-43fe-8d69-7d99f7e18ff2@emailsignatures365.codetwo.com> Message-ID: Good afternoon, I have been tasked to find a better solution to transport extremities from the OR to histology. We have tried cardboard boxes (no longer allowed) and plastic totes on rollers (too big to store and absorb odors from the legs). The histology lab is located quite a ways from the OR and passes through some main areas of the hospital. I was wondering what everybody else uses for this issue. All suggestions are appreciated. Thank you in advance for any ideas. Sincerely, Dawn Olszewski, HTL(ASCP)QIHC Pathology Manager South Georgia Medical Center 229-259-4830 dawn.olszewski at sgmc.org Dawn Olszewski Pathology Manager - Histotechnologist Laboratory [cid:MasterLogo_190x61_362365a5-c941-4f16-9f99-9484fd6a6078.png] South Georgia Medical Center 2501 N. Patterson St. Valdosta, GA 31602 229-259-4830 (O) | 229-560-6191 (M) Dawn.Olszewski at SGMC.ORG | sgmc.org [cid:facebook_fb_32x32_0a6d9065-09e0-48f7-88c4-b2d459a5e547.png] [cid:twitter_32x32_5a3a5eeb-77c7-4a3c-aa09-b69b41f32bd2.png] [cid:linkedin_ln_32x32_bf1e5dc9-b886-480a-8c8b-ef8a1ae87168.png] [cid:youtube_play_32x32_572cd3ed-390b-48e0-8845-ba1f81953de3.png] From kbowden at ucsd.edu Mon Oct 21 16:03:03 2019 From: kbowden at ucsd.edu (KD Bow) Date: Mon, 21 Oct 2019 14:03:03 -0700 Subject: [Histonet] Extremity carrier In-Reply-To: References: <12a3486f-aba0-466f-9fef-6a4ca11e0a30.3b5c5640-2195-4f35-b5ef-5e1ebee68512.5202a337-b4de-43fe-8d69-7d99f7e18ff2@emailsignatures365.codetwo.com> Message-ID: <85980308-6efc-2db6-8b5c-a17faef97dc7@ucsd.edu> I work in a research lab so this may not go over well.? How about using a cart and a child size body bag? You would not be able to see through the bag and there shouldn't be any smell and you most likely have a cart around the place. -- */-- You are what you do - not what you say --/* *Karen Bowden Staff Research Associate II University of CA, San Diego* *Department of Medicine* *9500 Gilman Dr. 0682* *La Jolla, CA 92093-0682* *858-534-0575 voice 858-534-2005 fax **/ CONFIDENTIALITY NOTICE: /**/THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED.IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER/**/./* On 10/21/19 1:19 PM, Olszewski, Dawn via Histonet wrote: > Good afternoon, > > I have been tasked to find a better solution to transport extremities from the OR to histology. We have tried cardboard boxes (no longer allowed) and plastic totes on rollers (too big to store and absorb odors from the legs). The histology lab is located quite a ways from the OR and passes through some main areas of the hospital. I was wondering what everybody else uses for this issue. All suggestions are appreciated. Thank you in advance for any ideas. > > Sincerely, > > Dawn Olszewski, HTL(ASCP)QIHC > Pathology Manager > South Georgia Medical Center > 229-259-4830 > dawn.olszewski at sgmc.org > > > > Dawn Olszewski > Pathology Manager - Histotechnologist > Laboratory > > [cid:MasterLogo_190x61_362365a5-c941-4f16-9f99-9484fd6a6078.png] > > South Georgia Medical Center > 2501 N. Patterson St. > Valdosta, GA 31602 > 229-259-4830 (O) | 229-560-6191 (M) > Dawn.Olszewski at SGMC.ORG | sgmc.org > > [cid:facebook_fb_32x32_0a6d9065-09e0-48f7-88c4-b2d459a5e547.png] [cid:twitter_32x32_5a3a5eeb-77c7-4a3c-aa09-b69b41f32bd2.png] [cid:linkedin_ln_32x32_bf1e5dc9-b886-480a-8c8b-ef8a1ae87168.png] [cid:youtube_play_32x32_572cd3ed-390b-48e0-8845-ba1f81953de3.png] > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen at parrishmed.com Tue Oct 22 05:01:07 2019 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Tue, 22 Oct 2019 10:01:07 +0000 Subject: [Histonet] [EXTERNAL Sender] Re: Extremity carrier In-Reply-To: <85980308-6efc-2db6-8b5c-a17faef97dc7@ucsd.edu> References: <12a3486f-aba0-466f-9fef-6a4ca11e0a30.3b5c5640-2195-4f35-b5ef-5e1ebee68512.5202a337-b4de-43fe-8d69-7d99f7e18ff2@emailsignatures365.codetwo.com> <85980308-6efc-2db6-8b5c-a17faef97dc7@ucsd.edu> Message-ID: <71536df8e8394228bdff672da08ac7c5@PMSVRLEX02.parrishmed.local> We have a "Pathport" container ( kind of large) that we use, we place it on a (designated) cart. When we are done with the extremity, we place it in a large (designated) biohazard (sharps) container which we store in our walk-in refrigerator until time for disposal. We return the "Pathport" container and cart to our Sterile processing department, they decontaminate the "Pathport" and it and the cart are returned to the O.R. area. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: KD Bow via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, October 21, 2019 5:03 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL Sender] Re: [Histonet] Extremity carrier This message came from an external source. Please do not click links or open attachments if unexpected or unusual. Begin Original Message: ---------------------------------------------------------------------- I work in a research lab so this may not go over well.? How about using a cart and a child size body bag? You would not be able to see through the bag and there shouldn't be any smell and you most likely have a cart around the place. -- */-- You are what you do - not what you say --/* *Karen Bowden Staff Research Associate II University of CA, San Diego* *Department of Medicine* *9500 Gilman Dr. 0682* *La Jolla, CA 92093-0682* *858-534-0575 voice 858-534-2005 fax **/ CONFIDENTIALITY NOTICE: /**/THE INFORMATION TRANSMITTED IN THIS E-MAIL IS INTENDED ONLY FOR THE PERSON OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN CONFIDENTIAL AND/OR PRIVILEGED MATERIAL. ANY REVIEW, RETRANSMISSION, DISSEMINATION OR OTHER USE OF OR TAKING OF ANY ACTION IN RELIANCE UPON THIS INFORMATION BY PERSONS OR ENTITIES OTHER THAN THE INTENDED RECIPIENT IS PROHIBITED.IF YOU RECEIVED THIS E-MAIL IN ERROR, PLEASE CONTACT THE SENDER AND DELETE THE MATERIAL FROM ANY COMPUTER/**/./* On 10/21/19 1:19 PM, Olszewski, Dawn via Histonet wrote: > Good afternoon, > > I have been tasked to find a better solution to transport extremities from the OR to histology. We have tried cardboard boxes (no longer allowed) and plastic totes on rollers (too big to store and absorb odors from the legs). The histology lab is located quite a ways from the OR and passes through some main areas of the hospital. I was wondering what everybody else uses for this issue. All suggestions are appreciated. Thank you in advance for any ideas. > > Sincerely, > > Dawn Olszewski, HTL(ASCP)QIHC > Pathology Manager > South Georgia Medical Center > 229-259-4830 > dawn.olszewski at sgmc.org > > > > Dawn Olszewski > Pathology Manager - Histotechnologist > Laboratory > > [cid:MasterLogo_190x61_362365a5-c941-4f16-9f99-9484fd6a6078.png] > > South Georgia Medical Center > 2501 N. Patterson St. > Valdosta, GA 31602 > 229-259-4830 (O) | 229-560-6191 (M) > Dawn.Olszewski at SGMC.ORG | sgmc.org > > [cid:facebook_fb_32x32_0a6d9065-09e0-48f7-88c4-b2d459a5e547.png] [cid:twitter_32x32_5a3a5eeb-77c7-4a3c-aa09-b69b41f32bd2.png] [cid:linkedin_ln_32x32_bf1e5dc9-b886-480a-8c8b-ef8a1ae87168.png] [cid:youtube_play_32x32_572cd3ed-390b-48e0-8845-ba1f81953de3.png] > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=Xk0H5Lt1SX4yLDi3Z36FWCjxWMexCFmv9kQz5nprqM4&r=S0NRG57vjoVygxIXrxrniUhxWkoaXBwDrgVZ_u7KUmY&m=f6_WJrhufuW5Qo7X45nVQslVMjxRF5DR95QwP24Ow-A&s=UZEK7AjLw9FtJYtZhoe_hRiwuNtZuHqDVUj1Ch6ckpU&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=Xk0H5Lt1SX4yLDi3Z36FWCjxWMexCFmv9kQz5nprqM4&r=S0NRG57vjoVygxIXrxrniUhxWkoaXBwDrgVZ_u7KUmY&m=f6_WJrhufuW5Qo7X45nVQslVMjxRF5DR95QwP24Ow-A&s=UZEK7AjLw9FtJYtZhoe_hRiwuNtZuHqDVUj1Ch6ckpU&e= From bedubya84 at aol.com Tue Oct 22 08:30:09 2019 From: bedubya84 at aol.com (Brandon Ward) Date: Tue, 22 Oct 2019 13:30:09 +0000 (UTC) Subject: [Histonet] Message to Histonet List Members References: <152646400.3400245.1571751009343.ref@mail.yahoo.com> Message-ID: <152646400.3400245.1571751009343@mail.yahoo.com> Hello,?Would you please post the following message from me to the list members on the histonet?:?Hello all, I'm wondering if any health systems have a quality program that includes aperson(s) who audit final gross and final pathology reports? This is differentfrom auditing to ensure LIS information is crossing over correctly.This?would be to ensure no errors were?made by grossing staff andpathologists.? If so, how is this auditing performed? Do pathologists self-audit? Is there atranscriptionist(s) who perform the auditing and what percentage of cases (boththe gross and final) are audited? Thanks in advance for your help! Thanks!Brandon WardHistology Education CoordinatorACL LaboratoriesWest Allis, WI?? From b-frederick at northwestern.edu Tue Oct 22 10:50:02 2019 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Tue, 22 Oct 2019 15:50:02 +0000 Subject: [Histonet] RAt teeth Message-ID: Hi all, I have bamboo rat teeth that have (ha-ha) been decalled. Obviously they are not soft. Can I use Nair on them or is there something better? I will need an H&E and an iron stain. They are not tiny samples...... Bernice Bernice Frederick Pathology Core Facility Robert H. Lurie Cancer Center 710 North Fairbanks Court Olson 8-421 312-503-3723 b-frederick at northwestern.edu From rsrichmond at gmail.com Tue Oct 22 12:50:59 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Tue, 22 Oct 2019 13:50:59 -0400 Subject: [Histonet] Extremity carrier In-Reply-To: References: Message-ID: Dawn Olszewski asks: >>I have been tasked to find a better solution to transport extremities from the OR to histology. We have tried cardboard boxes (no longer allowed) and plastic totes on rollers (too big to store and absorb odors from the legs).<< Mopec offers >>Pathport 3 is a stainless steel constructed transport and storage system specifically manufactured to accommodate pathology specimens. Equipped with a custom fit disposable formaldehyde-neutralizing pad and includes safety locks. The Pathport 3 is specially designed to absorb harmful spills and vapors. Spill clean-up can be accomplished by simply replacing the pad.<< I didn't check the price of the Pathport 3, but it's difficult to imagine a lab manager willing to spring for what must be a very expensive item. Particularly because you'd need several of them, since pathologists are accustomed to letting dead legs sit around for several days before they dissect them, and then insisting the specimen sit around until the slides are prepared and the case is signed out and forgotten. I always raised people's eyebrows by insisting on dissecting the specimen the day it arrived, and insisting it was off to the incinerator as soon as I was done dissecting. I'd fix the tissues I wanted to examine, in a small pot of formalin overnight, block them the following morning, decalcify the arteries, and into the processor the next evening. Bob Richmond Samurai Pathologist Maryville TN From tbraud at holyredeemer.com Tue Oct 22 13:03:05 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 22 Oct 2019 18:03:05 +0000 Subject: [Histonet] Transporting large amputations and QA review of reports Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B63D8E@HRHEX02-HOS.holyredeemer.local> 1. Extremity carrier (Olszewski, Dawn) I completely agree with Karen Bowden's response, "I work in a research lab so this may not go over well.? How about using a cart and a child size body bag? You would not be able to see through the bag and there shouldn't be any smell and you most likely have a cart around the place" This is exactly what we do. The process is clean and neat and we also have to transport it through multiple patient areas. 4. Brandon Ward asked, "I'm wondering if any health systems have a quality program that includes a person(s) who audit final gross and final pathology reports?" Our QA process reviews all of our reports that should contain synoptic reports. These are reviewed by the Chief pathologist for the accuracy of the report information including the synoptic reports. Just my 2 cents, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From KBummer at nektar.com Thu Oct 24 13:49:53 2019 From: KBummer at nektar.com (Bummer, Katherine) Date: Thu, 24 Oct 2019 18:49:53 +0000 Subject: [Histonet] Questions on how to operate a ThermoFisher Lab Vision autostainer 3602D Message-ID: <2fed8f677f9540caaf6ac09fe763402d@nektar.com> Hello Histonetters, My company has acquired a refurbished ThermoFisher Lab Vision autostainer 3602D and I have questions on how to operate. It is no longer supported by ThermoFisher so I am unable to get tech support through them. Is there anyone who is familiar and available/willing to answer a few questions? Thank you in advance. Kate Katherine Bummer Nektar Therapeutics 455 Mission Bay Boulevard South San Francisco, CA 94158 K.Bummer at Nektar.com 415.482.5716 From akemiat3377 at gmail.com Thu Oct 24 14:42:53 2019 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Thu, 24 Oct 2019 13:42:53 -0600 Subject: [Histonet] Questions on how to operate a ThermoFisher Lab Vision autostainer 3602D In-Reply-To: <2fed8f677f9540caaf6ac09fe763402d@nektar.com> References: <2fed8f677f9540caaf6ac09fe763402d@nektar.com> Message-ID: <59BB85C1-D7C9-46C7-A2CE-E64D63F132A7@gmail.com> Ask Biocare or Dako. Unbeknownst to most of the public, it is the same exact unit they had years ago. Lab Vision made all 3 units. Their brand, Biocare and Dako. Lab Vision just put different branding on the various units when they were sold. Akemi Allison-Tacha, BS, HT/HTL (ASCP) Former Director and Operations Manager at Histology at Biocare > On Oct 24, 2019, at 12:49 PM, Bummer, Katherine via Histonet wrote: > > Hello Histonetters, > > My company has acquired a refurbished ThermoFisher Lab Vision autostainer 3602D and I have questions on how to operate. It is no longer supported by ThermoFisher so I am unable to get tech support through them. Is there anyone who is familiar and available/willing to answer a few questions? > > Thank you in advance. > > Kate > > Katherine Bummer > Nektar Therapeutics > 455 Mission Bay Boulevard South > San Francisco, CA 94158 > K.Bummer at Nektar.com > 415.482.5716 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster at umn.edu Thu Oct 24 15:08:35 2019 From: cforster at umn.edu (Colleen Forster) Date: Thu, 24 Oct 2019 15:08:35 -0500 Subject: [Histonet] Questions on how to operate a ThermoFisher Lab Vision autostainer 3602D In-Reply-To: <59BB85C1-D7C9-46C7-A2CE-E64D63F132A7@gmail.com> References: <2fed8f677f9540caaf6ac09fe763402d@nektar.com> <59BB85C1-D7C9-46C7-A2CE-E64D63F132A7@gmail.com> Message-ID: The Biocare certain was called the Nemesis. They are phasibg them out but might be willing to come assist you. Colleen Forster On Thu, Oct 24, 2019, 2:43 PM Eileen Akemi Allison via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Ask Biocare or Dako. Unbeknownst to most of the public, it is the same > exact unit they had years ago. Lab Vision made all 3 units. Their brand, > Biocare and Dako. Lab Vision just put different branding on the various > units when they were sold. > > Akemi Allison-Tacha, BS, HT/HTL (ASCP) > Former Director and Operations Manager at Histology at Biocare > > > On Oct 24, 2019, at 12:49 PM, Bummer, Katherine via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > > Hello Histonetters, > > > > My company has acquired a refurbished ThermoFisher Lab Vision > autostainer 3602D and I have questions on how to operate. It is no longer > supported by ThermoFisher so I am unable to get tech support through them. > Is there anyone who is familiar and available/willing to answer a few > questions? > > > > Thank you in advance. > > > > Kate > > > > Katherine Bummer > > Nektar Therapeutics > > 455 Mission Bay Boulevard South > > San Francisco, CA 94158 > > K.Bummer at Nektar.com > > 415.482.5716 > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From criley at dpspa.com Fri Oct 25 07:12:50 2019 From: criley at dpspa.com (Charles Riley) Date: Fri, 25 Oct 2019 08:12:50 -0400 Subject: [Histonet] Cell block processing Message-ID: Does anyone have any tips or suggestions on how to better process extremely bloody FNA specimens? Is there anyway to clear out some or all of the blood without destroying the other tissues? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From criley at dpspa.com Fri Oct 25 08:09:44 2019 From: criley at dpspa.com (Charles Riley) Date: Fri, 25 Oct 2019 09:09:44 -0400 Subject: [Histonet] Instrument feed water Message-ID: Where does everyone purchase their IFW from? or how do you produce it in house? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From BMolinari at texasheart.org Fri Oct 25 08:49:54 2019 From: BMolinari at texasheart.org (Betsy Molinari) Date: Fri, 25 Oct 2019 13:49:54 +0000 Subject: [Histonet] waterbath/paraffin References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.000bd4b9-5485-4e72-80a6-81fb60fa291d@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.356bb3b3-ad8e-4088-af00-ebec6203d580@emailsignatures365.codetwo.com> Message-ID: Hi, When I place my sections on the waterbath the paraffin pulls away from it. Leaving just the edge of the tissue. It is very weird. I have tried at different temps . The tissue itself is fine so I do not believe it is the processing. I use the same paraffin for processing as well as embedding. I use Fisher Histoplast IM. I was considering melting a block or two down and embedding them in another paraffin from another lab. Suggestions? Betsy Molinari HT, ASCP Texas Heart Institute 6770 Bertner Ave. O-511 Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From jwwalker at rrmc.org Fri Oct 25 11:23:43 2019 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Fri, 25 Oct 2019 16:23:43 +0000 Subject: [Histonet] Cell block processing In-Reply-To: References: Message-ID: Hi Charles, What are you collecting the FNA into? Cytorich? Cytolyt? Other? Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Charles Riley via Histonet Sent: Friday, October 25, 2019 8:13 AM To: Histo List Subject: [Histonet] Cell block processing [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. Does anyone have any tips or suggestions on how to better process extremely bloody FNA specimens? Is there anyway to clear out some or all of the blood without destroying the other tissues? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.com%2Fv3%2F__http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet__%3B!5JlSGkcjda6Eqs5J!rntZr6E7tVvCc2tzoJy_L8lFMF6EvHUTKJOsc3NTMLFSI35SgrcseW2ly3GyamY%24&data=02%7C01%7Cjwwalker%40rrmc.org%7C6e4cdd79edfa4e1ef23408d75944c458%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C637076024216531651&sdata=xAxqyyCfRepB3DlAl%2Fw651nk3B5ViHQLjqdToa2iAhw%3D&reserved=0 [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] From criley at dpspa.com Fri Oct 25 11:56:34 2019 From: criley at dpspa.com (Charles Riley) Date: Fri, 25 Oct 2019 12:56:34 -0400 Subject: [Histonet] Cell block processing In-Reply-To: References: Message-ID: Our tech said they use 95% alcohol to collect the specimen. On Fri, Oct 25, 2019 at 12:23 PM Joe W. Walker, Jr. wrote: > Hi Charles, > > What are you collecting the FNA into? Cytorich? Cytolyt? Other? > > Joe W. Walker, Jr. MS, SCT(ASCP) > Anatomical Pathology Manager > joewalker at rrmc.org, www.rrmc.org > > -----Original Message----- > From: Charles Riley via Histonet > Sent: Friday, October 25, 2019 8:13 AM > To: Histo List > Subject: [Histonet] Cell block processing > > [External Email] This email originated from outside of the organization. > Think before you click: Don?t click on links, open attachments or respond > to requests for sensitive information if the email looks suspicious or you > don?t recognize the sender. > > > Does anyone have any tips or suggestions on how to better process extremely > bloody FNA specimens? Is there anyway to clear out some or all of the > blood without destroying the other tissues? > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.com%2Fv3%2F__http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet__%3B!5JlSGkcjda6Eqs5J!rntZr6E7tVvCc2tzoJy_L8lFMF6EvHUTKJOsc3NTMLFSI35SgrcseW2ly3GyamY%24&data=02%7C01%7Cjwwalker%40rrmc.org%7C6e4cdd79edfa4e1ef23408d75944c458%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C637076024216531651&sdata=xAxqyyCfRepB3DlAl%2Fw651nk3B5ViHQLjqdToa2iAhw%3D&reserved=0 > [ > https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg > ] > -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From jeanine.ronkowski at yahoo.com Fri Oct 25 14:48:39 2019 From: jeanine.ronkowski at yahoo.com (Jeanine Ronkowski) Date: Fri, 25 Oct 2019 19:48:39 +0000 (UTC) Subject: [Histonet] Questions on how to operate a ThermoFisher Lab Vision autostainer 3602D References: <1114112305.1196632.1572032919300.ref@mail.yahoo.com> Message-ID: <1114112305.1196632.1572032919300@mail.yahoo.com> Katherine:Our lab did the same thing a few years ago and I received operating instructions for this system from a sales rep with Biocare that I know.? I'll send this document to you offline.? I can also put you in touch with the guy from Biocare.? As Colleen said, Biocare doesn't sell or support this instrumemt any more, but you can still get reagents from them.? I know of only three companies that can provide service and PMs.? Let me know if you need that info too.Good Luck,Jeanine (Jeanine.Ronkowski at yahoo.com) * * * * * * * * * * * * * * * Hello Histonetters, My company has acquired a refurbished ThermoFisher Lab Vision autostainer 3602D and I have questions on how to operate.? It is no longer supported by ThermoFisher so I am unable to get tech support through them.? Is there anyone who is familiar and available/willing to answer a few questions? Thank you in advance. Kate Katherine Bummer Nektar Therapeutics 455 Mission Bay Boulevard South San Francisco, CA? 94158 K.Bummer at Nektar.com 415.482.5716 From jwwalker at rrmc.org Fri Oct 25 15:35:34 2019 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Fri, 25 Oct 2019 20:35:34 +0000 Subject: [Histonet] Cell block processing In-Reply-To: References: Message-ID: As a cytotech, that wouldn?t be my first choice for collections and FNA specimens. The main reason is that once fixed in 95% ETOH you are limited if you need to perform IHC stains on the cell block unless you have validated your IHCs on ETOH fixed specimens. How do you process the FNA rinses that in in ETOH: Only Cell blocks or do you have another cytology liquid prep? Without knowing your prep process, I?d suggest collecting the FNA needle rinses in Hank?s Balanced Salt solution. After making the cell block, you could then formalin fix them. I can send you a procedure that we utilize for this process. The cell blocks cut great, look great, and you can perform IHC an molecular testing if needed. Joe Walker From: Charles Riley Sent: Friday, October 25, 2019 12:57 PM To: Joe W. Walker, Jr. Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Cell block processing [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. Our tech said they use 95% alcohol to collect the specimen. On Fri, Oct 25, 2019 at 12:23 PM Joe W. Walker, Jr. > wrote: Hi Charles, What are you collecting the FNA into? Cytorich? Cytolyt? Other? Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Charles Riley via Histonet > Sent: Friday, October 25, 2019 8:13 AM To: Histo List > Subject: [Histonet] Cell block processing [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. Does anyone have any tips or suggestions on how to better process extremely bloody FNA specimens? Is there anyway to clear out some or all of the blood without destroying the other tissues? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.com%2Fv3%2F__http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet__%3B!5JlSGkcjda6Eqs5J!rntZr6E7tVvCc2tzoJy_L8lFMF6EvHUTKJOsc3NTMLFSI35SgrcseW2ly3GyamY%24&data=02%7C01%7Cjwwalker%40rrmc.org%7C6e4cdd79edfa4e1ef23408d75944c458%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C637076024216531651&sdata=xAxqyyCfRepB3DlAl%2Fw651nk3B5ViHQLjqdToa2iAhw%3D&reserved=0 [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From Richard.Cartun at hhchealth.org Fri Oct 25 16:08:11 2019 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 25 Oct 2019 21:08:11 +0000 Subject: [Histonet] Cell block processing In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EC7CE18E9@HHCEXCHMB03.hhcsystem.org> I agree with Joe. We used to use ETOH for cell blocks, but stopped using it when we started doing IHC biomarker testing on these specimens. Alcohol is good for some proteomic targets, but can be a disaster for others. We also fix all of our cell block specimens that are collected in saline or RPMI in formalin. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org -----Original Message----- From: Joe W. Walker, Jr. via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, October 25, 2019 4:36 PM To: Charles Riley Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Cell block processing EXTERNAL email is from outside HHC. DO NOT open attachments or click links from unknown senders. As a cytotech, that wouldn?t be my first choice for collections and FNA specimens. The main reason is that once fixed in 95% ETOH you are limited if you need to perform IHC stains on the cell block unless you have validated your IHCs on ETOH fixed specimens. How do you process the FNA rinses that in in ETOH: Only Cell blocks or do you have another cytology liquid prep? Without knowing your prep process, I?d suggest collecting the FNA needle rinses in Hank?s Balanced Salt solution. After making the cell block, you could then formalin fix them. I can send you a procedure that we utilize for this process. The cell blocks cut great, look great, and you can perform IHC an molecular testing if needed. Joe Walker From: Charles Riley Sent: Friday, October 25, 2019 12:57 PM To: Joe W. Walker, Jr. Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Cell block processing [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. Our tech said they use 95% alcohol to collect the specimen. On Fri, Oct 25, 2019 at 12:23 PM Joe W. Walker, Jr. > wrote: Hi Charles, What are you collecting the FNA into? Cytorich? Cytolyt? Other? Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewalker at rrmc.org, https://urldefense.proofpoint.com/v2/url?u=http-3A__www.rrmc.org&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=2CdTK8PmO1-CXFcs6R-WWhnxgbCm1AHZ9wJ_1YOqDoA&s=JIavq_Wfz4S4TppCsMv8kQfnmD9wLQhRVNqSm1eJAO4&e= -----Original Message----- From: Charles Riley via Histonet > Sent: Friday, October 25, 2019 8:13 AM To: Histo List > Subject: [Histonet] Cell block processing [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. Does anyone have any tips or suggestions on how to better process extremely bloody FNA specimens? Is there anyway to clear out some or all of the blood without destroying the other tissues? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=https-3A__nam02.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.com-252Fv3-252F-5F-5Fhttp-253A-252F-252Flists.utsouthwestern.edu-252Fmailman-252Flistinfo-252Fhistonet-5F-5F-253B-215JlSGkcjda6Eqs5J-21rntZr6E7tVvCc2tzoJy-5FL8lFMF6EvHUTKJOsc3NTMLFSI35SgrcseW2ly3GyamY-2524-26amp-3Bdata-3D02-257C01-257Cjwwalker-2540rrmc.org-257C6e4cdd79edfa4e1ef23408d75944c458-257C0e55647d438e4a448437e959c3cf2240-257C0-257C0-257C637076024216531651-26amp-3Bsdata-3DxAxqyyCfRepB3DlAl-252Fw651nk3B5ViHQLjqdToa2iAhw-253D-26amp-3Breserved-3D0&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=2CdTK8PmO1-CXFcs6R-WWhnxgbCm1AHZ9wJ_1YOqDoA&s=RobiJPUYXo7gJ0eE4FcEyESLjuvd538sf63XqDz_iQM&e= [https://urldefense.proofpoint.com/v2/url?u=https-3A__www.rrmc.org_app_files_public_2633_2019-5Fhyht-5Fsig-2D-5Fjan2019-5Ffinal.jpg&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=2CdTK8PmO1-CXFcs6R-WWhnxgbCm1AHZ9wJ_1YOqDoA&s=bfdUjlkLuoXlexR_6MKvvmNZpyWed0IpYcZ2X9GEJso&e= ] -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=2CdTK8PmO1-CXFcs6R-WWhnxgbCm1AHZ9wJ_1YOqDoA&s=cGbNBd2_FLuEpWF_WFTt0WLrdS4gyklejRQQU9zpG7M&e= Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From tony.henwood at health.nsw.gov.au Fri Oct 25 16:15:05 2019 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 25 Oct 2019 21:15:05 +0000 Subject: [Histonet] waterbath/paraffin In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.3ae397c8-e096-4897-bf5b-660c6f990de7.000bd4b9-5485-4e72-80a6-81fb60fa291d@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.356bb3b3-ad8e-4088-af00-ebec6203d580@emailsignatures365.codetwo.com>, Message-ID: <1572038105541.1772@health.nsw.gov.au> Are the tissues taken directly from the molten wax and placed in the wax-containing mold or are they allowed to cool before embedding? One cause of tissue separation is the difference in temperature between tissue and embedding wax. Try taking directly from molten wax and embedding directly in the wax-filled mold. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Betsy Molinari via Histonet Sent: Saturday, 26 October 2019 00:49 To: 'Histonet at lists.utsouthwestern.edu' Subject: [Histonet] waterbath/paraffin Hi, When I place my sections on the waterbath the paraffin pulls away from it. Leaving just the edge of the tissue. It is very weird. I have tried at different temps . The tissue itself is fine so I do not believe it is the processing. I use the same paraffin for processing as well as embedding. I use Fisher Histoplast IM. I was considering melting a block or two down and embedding them in another paraffin from another lab. Suggestions? Betsy Molinari HT, ASCP Texas Heart Institute 6770 Bertner Ave. O-511 Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From tony.henwood at health.nsw.gov.au Fri Oct 25 18:50:10 2019 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 25 Oct 2019 23:50:10 +0000 Subject: [Histonet] Cell block processing In-Reply-To: References: Message-ID: <1572047411440.26917@health.nsw.gov.au> Hi Charles, I have had excellent success with lysing the red blood cells (using Isotonic Ammonium Chloride) prior to cell block preparation with thromboplastin-plasma. The lysing solution contains EDTA so you will need to add a few drops of 1% calcium chloride. Method as follows: Lysis solution Ammonium Chloride 4.5g Potassium carbonate 0.5g EDTA 0.0186g Distilled water 500mls Method: 1. Centrifuge bloody fluid. 2. Remove supernatant and add equal volume of lysis solution. 3. Resuspend and incubate for 5 minutes at 4oC. 4. Centrifuge, if blood still remains, then repeat from step 2. 5. Rinse in Hanks or RPMI, centrifuge. 6. Mix pellet in a few drops of plasma. 7. Add thromboplastin and a few drops of 1% Calcium Chloride, mix gently and allow clot to form. 8. Add 10% buffered formalin and fix and process as usual. Reference: Kuenen-Boumiester etal (1996) Acta Cytolog 40:475-479 The lysis solution can also be purchased commercially from several companies (eg Biolegend). It is commonly used for sample preparation for flow cytometry. Check the SDS to make sure it does not contain formaldehyde. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Charles Riley via Histonet Sent: Friday, 25 October 2019 23:12 To: Histo List Subject: [Histonet] Cell block processing Does anyone have any tips or suggestions on how to better process extremely bloody FNA specimens? Is there anyway to clear out some or all of the blood without destroying the other tissues? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From criley at dpspa.com Mon Oct 28 09:02:33 2019 From: criley at dpspa.com (Charles Riley) Date: Mon, 28 Oct 2019 10:02:33 -0400 Subject: [Histonet] BRAF KRAS NRAS by PCR Message-ID: Does anyone do these tests using PCR on FFPE blocks? If so what platform do you use/recommend? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From tbraud at holyredeemer.com Mon Oct 28 09:14:14 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 28 Oct 2019 14:14:14 +0000 Subject: [Histonet] Cell block processing Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B64835@HRHEX02-HOS.holyredeemer.local> We collect our FNAs in CytoLyt. We also use it to wash all our non-gyn fluids, but then we fix the cell block "pellet" in formalin. We have had no problems with immunos, and are able to lyse the RBCs to provide a nice, clear specimen. Hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From criley at dpspa.com Mon Oct 28 09:20:28 2019 From: criley at dpspa.com (Charles Riley) Date: Mon, 28 Oct 2019 10:20:28 -0400 Subject: [Histonet] PLAG1 Message-ID: My pathologists want to bring this test in house. Can anyone suggest a company I can purchase this antibody for testing via IHC on the Leica Bond platform? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From jwwalker at rrmc.org Mon Oct 28 09:38:26 2019 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Mon, 28 Oct 2019 14:38:26 +0000 Subject: [Histonet] Cell block processing In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F801C1B64835@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F801C1B64835@HRHEX02-HOS.holyredeemer.local> Message-ID: Hi Terri, At one time we did the same thing but have changed our approach in light of the FDA's and CAP's view point on ASRs. The potential problem is that IHCs are all validated/tested by the manufacturer on FFPE tissue. By introducing methanol/ethanol as the first step in fixation, you potentially have altered the initial fixation steps. I've attended several meetings on this topic and have been advised to stop performing IHC on methanol/ethanol fixed specimens unless we validated that this fixation step doesn't alter the expression of the target antigen in the tissue. Formalin fixation after an alcohol fixation doesn't change/reverse any alterations to the antigen in the tissue. We utilize an IBF tissue fixative but have also validated this fixative with our antibody panels that we offer. The IBF does contain a small amount of alcohol and the fixative is slightly different than 10% buffered formalin. I agree that CytoLyt is excellent at lysing red blood cells but would just caution you on using the specimen for IHC without a disclaimer within your report or validating your IHCs on these specimens to ensure they work as expected. Keep in mind that most control tissue is FFPE and using it to compare if the IHC worked in a first fixed alcohol specimen is not an apples to apples comparison. Cheers, Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Terri Braud via Histonet Sent: Monday, October 28, 2019 10:14 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Cell block processing [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. We collect our FNAs in CytoLyt. We also use it to wash all our non-gyn fluids, but then we fix the cell block "pellet" in formalin. We have had no problems with immunos, and are able to lyse the RBCs to provide a nice, clear specimen. Hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.com%2Fv3%2F__http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet__%3B!5JlSGkcjda6Eqs5J!rHP2_dFONxwRmR87ddavejT_o6RIcXBah8jw7nMUO0tWdC5KpksUnk2bttyIMkU%24&data=02%7C01%7Cjwwalker%40rrmc.org%7Ceeac9b3afe444de27cd208d75bb1634b%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C637078689748526729&sdata=1%2BpvxH9TcvKxAo6jgjr7fobCAUUz35sHbsLfoBvHmGE%3D&reserved=0 [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] From Richard.Cartun at hhchealth.org Mon Oct 28 11:06:55 2019 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 28 Oct 2019 16:06:55 +0000 Subject: [Histonet] PLAG1 In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EC7CE1EB4@HHCEXCHMB03.hhcsystem.org> Looking at an article in Histopathology (January 2018) on PLAG1 IHC testing, the authors used clone "3B7" (Novus Biologicals in Littleton, CO) on the Bond Max IHC platform form. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, October 28, 2019 10:20 AM To: Histo List Subject: [Histonet] PLAG1 EXTERNAL email is from outside HHC. DO NOT open attachments or click links from unknown senders. My pathologists want to bring this test in house. Can anyone suggest a company I can purchase this antibody for testing via IHC on the Leica Bond platform? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=4VoB34evfULROSYTZirfyVjGhHd9khhhTv1g4pUQmxY&s=jqmKMH0dL56TvREZ4Edi5Oza2RVUTXB-PoqLdT5Sc2k&e= Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From john.garratt at ciqc.ca Mon Oct 28 16:01:39 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Mon, 28 Oct 2019 21:01:39 +0000 Subject: [Histonet] Cell block processing In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F801C1B64835@HRHEX02-HOS.holyredeemer.local> Message-ID: There's been excellent discussion on fixation, IHC and cells blocks, with Joe Walker's email summing up the situation nicely. For those interested in side by side comparison of IHC staining of cytology cell blocks using different fixatives go to http://cpqa.ca/main/wp-content/uploads/2014/06/2014-Thomson.pdf Regards John Garratt www.ciqc.ca ??????? Original Message ??????? On Monday, October 28, 2019 7:38 AM, Joe W. Walker, Jr. via Histonet wrote: > Hi Terri, > > At one time we did the same thing but have changed our approach in light of the FDA's and CAP's view point on ASRs. The potential problem is that IHCs are all validated/tested by the manufacturer on FFPE tissue. By introducing methanol/ethanol as the first step in fixation, you potentially have altered the initial fixation steps. I've attended several meetings on this topic and have been advised to stop performing IHC on methanol/ethanol fixed specimens unless we validated that this fixation step doesn't alter the expression of the target antigen in the tissue. Formalin fixation after an alcohol fixation doesn't change/reverse any alterations to the antigen in the tissue. > > We utilize an IBF tissue fixative but have also validated this fixative with our antibody panels that we offer. The IBF does contain a small amount of alcohol and the fixative is slightly different than 10% buffered formalin. > > I agree that CytoLyt is excellent at lysing red blood cells but would just caution you on using the specimen for IHC without a disclaimer within your report or validating your IHCs on these specimens to ensure they work as expected. Keep in mind that most control tissue is FFPE and using it to compare if the IHC worked in a first fixed alcohol specimen is not an apples to apples comparison. > > Cheers, > > Joe W. Walker, Jr. MS, SCT(ASCP) > Anatomical Pathology Manager > joewalker at rrmc.org, www.rrmc.org > > -----Original Message----- > From: Terri Braud via Histonet histonet at lists.utsouthwestern.edu > Sent: Monday, October 28, 2019 10:14 AM > To: 'histonet at lists.utsouthwestern.edu' histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Cell block processing > > [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. > > We collect our FNAs in CytoLyt. We also use it to wash all our non-gyn fluids, but then we fix the cell block "pellet" in formalin. > We have had no problems with immunos, and are able to lyse the RBCs to provide a nice, clear specimen. > Hope this helps. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://nam02.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.com%2Fv3%2F__http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet__%3B!5JlSGkcjda6Eqs5J!rHP2_dFONxwRmR87ddavejT_o6RIcXBah8jw7nMUO0tWdC5KpksUnk2bttyIMkU%24&data=02|01|jwwalker%40rrmc.org|eeac9b3afe444de27cd208d75bb1634b|0e55647d438e4a448437e959c3cf2240|0|0|637078689748526729&sdata=1%2BpvxH9TcvKxAo6jgjr7fobCAUUz35sHbsLfoBvHmGE%3D&reserved=0 > [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katherine at ka-recruiting.com Thu Oct 31 17:03:10 2019 From: katherine at ka-recruiting.com (Katherine Marano) Date: Thu, 31 Oct 2019 18:03:10 -0400 Subject: [Histonet] permanent and full-time histology openings Message-ID: Happy Halloween Histonetters! I am looking to fill some *permanent and full-time histology openings* asap. My clients offer frantastic compensation and benefits, as well as relocation assistance and sign on bonuses! Please let me know if you are interested in hearing more about any of these: Georgia - Histotech (3a-1130a) Illinois - Histotechnologist Indiana - Histotech openings (nights) North Carolina - Histotech North Carolina- Histology Techincian Trainee: Non Grosser (3rd shift) North Carolina - Histology Technician: Grossing North Dakota - Histology Lead (Monday-Friday day shift) New York - Histotech (3rd shift) Ohio - Histotech (Monday - Friday; day shift with varied start times) Oregon - Histotech HT or HTL (M-F day shift, IHC) Tennessee - Histology Supervisor (day shift but must be flexible to work others) Tennessee - Grosser (Evening/ night shift) Virginia - Histotech Wisconsin - Histotech (3rd shift ) Sincerely, Katherine Marano *K.A. 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