From melissa at alliedsearchpartners.com Wed May 1 15:53:37 2019 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Wed, 1 May 2019 20:53:37 +0000 Subject: [Histonet] Histology & IHC Applications Specialist Jobs in Boston Area Message-ID: Hello, just an update on two of my exclusive positions in the Boston area. They are Full Time/Permanent. Please reach out to me for more details if interested. Please note the heavy travel involved. This is not a typical bench role. So if you are interested in exploring your options outside of the bench, then call/text/email me for details! Thanks! 1. IHC Applications Specialist Must have recent and excellent IHC skills with ability to troubleshoot, set up equipment, etc. Traveling through the Boston and surround States to Massachusetts (75% of the time/25% work from home) Company car, phone, travel reimbursement (mileage/gas) 1. Applications Specialist Experience with confocal microscopy, brightfield microscopy, digital pathology A plus if you have flow cytometry and/or florescence experience Traveling through the Boston and surround States to Massachusetts (75% of the time/25% work from home) Company car, phone, travel reimbursement (mileage/gas) Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners Direct (Call) Line: 386.265.1368 Text Me: 386.855.8758 Toll Free: 888.388.7571 ext. 102 Fax: 888.388.7572 From LRaff at uropartners.com Fri May 3 10:26:56 2019 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 3 May 2019 15:26:56 +0000 Subject: [Histonet] Blog posting Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF1220D4E6@COLOEXCH01.uropartners.local> Thoughts on what comes after a career in the lab. http://www.chicagonow.com/downsize-maybe/2019/05/retire/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From pwnoyce at gmail.com Fri May 3 19:47:23 2019 From: pwnoyce at gmail.com (pwnoyce at gmail.com) Date: Sat, 4 May 2019 10:47:23 +1000 Subject: [Histonet] time for penetration of methacarn fixative Message-ID: <000f01d50212$f6465910$e2d30b30$@gmail.com> Does any one have data for the time it takes for methacarn fixative (60% methanol, 30% chloroform, 10% glacial acetic acid) to penetrate and then fully fix tissue? PW Noyce -PhD student From jkiernan at uwo.ca Sun May 5 23:26:24 2019 From: jkiernan at uwo.ca (John Kiernan) Date: Mon, 6 May 2019 04:26:24 +0000 Subject: [Histonet] time for penetration of methacarn fixative In-Reply-To: <000f01d50212$f6465910$e2d30b30$@gmail.com> References: <000f01d50212$f6465910$e2d30b30$@gmail.com> Message-ID: Peter Noyce, you did not say what tissue(s) you are planning to fix, or how big the specimens will be. Carnoy (1886) and methacarn (1970) were developed for animal tissues, cleverly balancing the actions of acetic acid and an alcohol in the presence of a hydrophobic solvent (chloroform) that diluted both these ingredients and also enhanced permeation by dissolving lipids. Carnoy's and other acid-alcohol fixatives are also used for plant specimens, which respond differently. If you are working with plant specimens you probably have a book by Steven E. Ruzin (1999) ISBN 0195089561. For the actions of alcohols and acetic acid in fixation of animal tissues, consult any text book of histotechnology or histochemistry published since about 1950. For selfish reasons, I recommend ISBN 9781907904325 (published in 2015) as an item to buy for your lab's bookshelf. Answers to your question are discussed in Chapter 5. With methacarn and other alcohol-acetic fixatives, no slow chemical reactions are involved (an important difference from formaldehyde-containing mixtures). Complete penetration accomplishes the fixation. The volume of fixative and procedure for subsequent processing into paraffin are very important. You need to read the paper by Puchtler et al (1970) and follow the instructions exactly. Probably you should also read Puchtler et al (1968) to use this type of non-aqueous fixative intelligently. PhD students are intelligent. I have added a couple of more recent papers that relate to methacarn. Read Puchtler or a textbook first. Not all modern investigators (especially molecular biologists) understand what the ingredients of fixative mixtures do to the different components of cells and extracellular materials. Current papers with micrographs full of ghastly artifacts abound, even in journals with very high citation indices. There are published mixtures with names like "modified methacarn" that may be OK for extracting RNA but do not have ingredients in correct proportions for minimizing distortion. Here's your list of recommended readings. - - - - - Puchtler H, Waldrop FS, Meloan SN, Terry MS, Connor HM (1970) Methacarn (methanol-Carnoy) fixation. Practical and theoretical considerations. Histochemie 21: 97-116. Puchtler H, Waldrop FS, Conner HM, Terry MS (1968) Carnoy fixation: practical and theoretical considerations. Histochemie 16: 361-371. Uneyama C, Shibutani M, Masutomi N, Takagi H, Hirose M (2002) Methacarn fixation for genomic DNA analysis in microdissected paraffin-embedded tissue specimens. J. Histochem. Cytochem. 50: 1237-1245. Buesa RJ (2008) Histology without formalin? Ann. Diagn. Path. 12: 387-396. - - - - - I wish you well with your research, and hope you will get your PhD while you are still young. John Kiernan London, Canada https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html http://biostain.com = = = ________________________________ From: peter noyce via Histonet Sent: 03 May 2019 19:47 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] time for penetration of methacarn fixative Does any one have data for the time it takes for methacarn fixative (60% methanol, 30% chloroform, 10% glacial acetic acid) to penetrate and then fully fix tissue? PW Noyce -PhD student _____________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff at uropartners.com Mon May 6 13:36:12 2019 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 6 May 2019 18:36:12 +0000 Subject: [Histonet] Looking for PRN second shift histologist Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF12220254@COLOEXCH01.uropartners.local> UroPartners Laboratory in Westchester, Illinois is seeking an experienced histologist for PRN second shift work, consisting mainly of cutting of biopsy specimens. If interested, please respond to me at lraff at uropartners.com Best regards, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From BMolinari at texasheart.org Tue May 7 05:46:01 2019 From: BMolinari at texasheart.org (Betsy Molinari) Date: Tue, 7 May 2019 10:46:01 +0000 Subject: [Histonet] time for penetration of methacarn fixative In-Reply-To: References: <000f01d50212$f6465910$e2d30b30$@gmail.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.a2f9f702-9a57-4a72-ac71-a5856150e07c.aa1d824f-2f9b-4639-b7f9-bd4d3bfae0e4@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.8af511b7-e036-4f00-b79e-b920f6c4d388@emailsignatures365.codetwo.com> Message-ID: John, You are such a wealth of information. I look forward to your posts. I learn something every time. That is going to be my reading list as well. Thank you. Betsy Molinari HT (ASCP) Texas Heart Institute 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Sr. Histology Research Technician CV Pathology Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter -----Original Message----- From: John Kiernan via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Sunday, May 05, 2019 11:26 PM To: pwnoyce at gmail.com Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] time for penetration of methacarn fixative *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ Peter Noyce, you did not say what tissue(s) you are planning to fix, or how big the specimens will be. Carnoy (1886) and methacarn (1970) were developed for animal tissues, cleverly balancing the actions of acetic acid and an alcohol in the presence of a hydrophobic solvent (chloroform) that diluted both these ingredients and also enhanced permeation by dissolving lipids. Carnoy's and other acid-alcohol fixatives are also used for plant specimens, which respond differently. If you are working with plant specimens you probably have a book by Steven E. Ruzin (1999) ISBN 0195089561. For the actions of alcohols and acetic acid in fixation of animal tissues, consult any text book of histotechnology or histochemistry published since about 1950. For selfish reasons, I recommend ISBN 9781907904325 (published in 2015) as an item to buy for your lab's bookshelf. Answers to your question are discussed in Chapter 5. With methacarn and other alcohol-acetic fixatives, no slow chemical reactions are involved (an important difference from formaldehyde-containing mixtures). Complete penetration accomplishes the fixation. The volume of fixative and procedure for subsequent processing into paraffin are very important. You need to read the paper by Puchtler et al (1970) and follow the instructions exactly. Probably you should also read Puchtler et al (1968) to use this type of non-aqueous fixative intelligently. PhD students are intelligent. I have added a couple of more recent papers that relate to methacarn. Read Puchtler or a textbook first. Not all modern investigators (especially molecular biologists) understand what the ingredients of fixative mixtures do to the different components of cells and extracellular materials. Current papers with micrographs full of ghastly artifacts abound, even in journals with very high citation indices. There are published mixtures with names like "modified methacarn" that may be OK for extracting RNA but do not have ingredients in correct proportions for minimizing distortion. Here's your list of recommended readings. - - - - - Puchtler H, Waldrop FS, Meloan SN, Terry MS, Connor HM (1970) Methacarn (methanol-Carnoy) fixation. Practical and theoretical considerations. Histochemie 21: 97-116. Puchtler H, Waldrop FS, Conner HM, Terry MS (1968) Carnoy fixation: practical and theoretical considerations. Histochemie 16: 361-371. Uneyama C, Shibutani M, Masutomi N, Takagi H, Hirose M (2002) Methacarn fixation for genomic DNA analysis in microdissected paraffin-embedded tissue specimens. J. Histochem. Cytochem. 50: 1237-1245. Buesa RJ (2008) Histology without formalin? Ann. Diagn. Path. 12: 387-396. - - - - - I wish you well with your research, and hope you will get your PhD while you are still young. John Kiernan London, Canada https://urldefense.proofpoint.com/v2/url?u=https-3A__www.schulich.uwo.ca_anatomy_people_bios_emeriti_kiernan-5Fjohn.html&d=DwICAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=WmaHc4fCJS42J-KsPVJSnE9N1rCFdozPF9DSJA90hT8&s=v6m2a9-Ol7YLjPL_ZAaTvLmF4mPHwe1z57iL7Vo_VAI&e= https://urldefense.proofpoint.com/v2/url?u=http-3A__biostain.com&d=DwICAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=WmaHc4fCJS42J-KsPVJSnE9N1rCFdozPF9DSJA90hT8&s=QPHVD0tPdeuvGeqJKyIsaA2Vv7HHNSCMi8LZw2MgKHE&e= = = = ________________________________ From: peter noyce via Histonet Sent: 03 May 2019 19:47 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] time for penetration of methacarn fixative Does any one have data for the time it takes for methacarn fixative (60% methanol, 30% chloroform, 10% glacial acetic acid) to penetrate and then fully fix tissue? PW Noyce -PhD student _____________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=WmaHc4fCJS42J-KsPVJSnE9N1rCFdozPF9DSJA90hT8&s=1lp9uIKaLTe8KPK9ZFYlC2-SOhvLNBGdrTdnF-KTIxA&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=WmaHc4fCJS42J-KsPVJSnE9N1rCFdozPF9DSJA90hT8&s=1lp9uIKaLTe8KPK9ZFYlC2-SOhvLNBGdrTdnF-KTIxA&e= This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From greg.dobbin at gmail.com Tue May 7 14:07:08 2019 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Tue, 7 May 2019 16:07:08 -0300 Subject: [Histonet] Processing Schedule- ASP-6025 Message-ID: Hello colleagues, I recently stained (IHC) a section of normal tonsil from another facility with p16 and the resulting stain was better than the same stain on a section of my labs own normal tonsil control. This has led us to question our processing schedule. I am not concerned with our fixation because we fix everything for at least 24 hours in 10% formalin (commercially prepared) prior to processing. Does anything jump out at you as being a potential red flag in the following overnight protocol? - Formalin 15 mins; RT - Processing water 1 min; RT - ETOH 70% 30 mins; 35C - ETOH 80% 30 mins; 35C - ETOH 95% 30 mins; 35C - ETOH 100% 30 mins; 35C - ETOH 100% 40 mins; 35C - ETOH 100% 60 mins; 35C - Xylene 60 mins; 35C - Xylene 60 mins; 35C - Xylene 60 mins; 35C - Paraffin 60 mins; 57C; vacuum - Paraffin 60 mins; 57C; vacuum - Paraffin 60 mins; 57C; vacuum Our formalin is changed after every 1100 cassettes and the alcohol, xylenes and paraffins are managed similarly by the instrument. Our specimen mix is a little of everything (skins, GIs, breasts, needle cores, gall bladders, gyne, etc). The one unknown (so far) in this story, is how the tonsil from the other laboratory was handled (ie the fixative used and for how long-I am assuming 10% formalin). Obviously, many of you will have schedules that differ from this one, in any number of ways, but what I am looking for from you is your opinion: *is there anything about this schedule that is particularly concerning?* Thank you, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From cls71877 at gmail.com Tue May 7 16:16:03 2019 From: cls71877 at gmail.com (Cristi Rigazio) Date: Tue, 7 May 2019 17:16:03 -0400 Subject: [Histonet] Benchmarks for Reprocessing Message-ID: Good afternoon Fellow histonetters! I was wondering if there are any tertiary institutions out there that have set a benchmark for reprocessing tissue? We tend to be less than 1%, but would love to see what others think is reasonable. Thanks for the feedback and hope you all are having an excellent day! Cristi From tony.henwood at health.nsw.gov.au Tue May 7 19:37:14 2019 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 8 May 2019 00:37:14 +0000 Subject: [Histonet] Processing Schedule- ASP-6025 In-Reply-To: References: Message-ID: <665e381823e244b7b6385deeb9dfaa45@UNDCMBX-MEX023.nswhealth.net> Processing seems adequate. After processing, how long do they sit in the embedding centre block holding tank before embedding? We found that quite a few antigens were affected when we stored control tonsil in the embedding centre (dry) at 60oC for a few days before embedding. In summary: Antibody Clone Dried (Normal = 3+) CD4 4B12 0 BOB-1 TG14 0 CD3 LN10 1+ CD79a JCB117 1+ Oct-2 Oct-207 1+ CD8 4B11 2+ CD20 L26 3+ So CD20 was unaffected but this process affected most of the antigens with some losing antigen recognition by the antibody (eg CD4 and BOB-1). Another one of those pre-analytical issues we need to consider. And yes I am writing this up for submission! Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) | Principal Scientist; Adjunct Fellow, School of Medicine, University of Western Sydney; Visiting Lecturer, School of Life Sciences, Faculty of Science, University of Technology Sydney | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: tony.henwood at health.nsw.gov.au | w: www.schn.health.nsw.gov.au m: Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ???Please consider the environment before printing this email. -----Original Message----- From: Greg Dobbin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 8 May 2019 5:07 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Processing Schedule- ASP-6025 Hello colleagues, I recently stained (IHC) a section of normal tonsil from another facility with p16 and the resulting stain was better than the same stain on a section of my labs own normal tonsil control. This has led us to question our processing schedule. I am not concerned with our fixation because we fix everything for at least 24 hours in 10% formalin (commercially prepared) prior to processing. Does anything jump out at you as being a potential red flag in the following overnight protocol? - Formalin 15 mins; RT - Processing water 1 min; RT - ETOH 70% 30 mins; 35C - ETOH 80% 30 mins; 35C - ETOH 95% 30 mins; 35C - ETOH 100% 30 mins; 35C - ETOH 100% 40 mins; 35C - ETOH 100% 60 mins; 35C - Xylene 60 mins; 35C - Xylene 60 mins; 35C - Xylene 60 mins; 35C - Paraffin 60 mins; 57C; vacuum - Paraffin 60 mins; 57C; vacuum - Paraffin 60 mins; 57C; vacuum Our formalin is changed after every 1100 cassettes and the alcohol, xylenes and paraffins are managed similarly by the instrument. Our specimen mix is a little of everything (skins, GIs, breasts, needle cores, gall bladders, gyne, etc). The one unknown (so far) in this story, is how the tonsil from the other laboratory was handled (ie the fixative used and for how long-I am assuming 10% formalin). Obviously, many of you will have schedules that differ from this one, in any number of ways, but what I am looking for from you is your opinion: *is there anything about this schedule that is particularly concerning?* Thank you, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From greg.dobbin at gmail.com Tue May 7 19:52:41 2019 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Tue, 7 May 2019 21:52:41 -0300 Subject: [Histonet] Processing Schedule- ASP-6025 In-Reply-To: <665e381823e244b7b6385deeb9dfaa45@UNDCMBX-MEX023.nswhealth.net> References: <665e381823e244b7b6385deeb9dfaa45@UNDCMBX-MEX023.nswhealth.net> Message-ID: Fascinating Tony! We don?t typically leave them in the warming drawer any longer than a couple of hours, but maybe this particular tonsil was left linger for some reason and no one thought anything of it?! Something to consider for sure! Thanks. Greg On Tue, May 7, 2019 at 9:37 PM Tony Henwood (SCHN) < tony.henwood at health.nsw.gov.au> wrote: > Processing seems adequate. > > After processing, how long do they sit in the embedding centre block > holding tank before embedding? > > We found that quite a few antigens were affected when we stored control > tonsil in the embedding centre (dry) at 60oC for a few days before > embedding. In summary: > > Antibody Clone Dried (Normal = 3+) > CD4 4B12 0 > BOB-1 TG14 0 > CD3 LN10 1+ > CD79a JCB117 1+ > Oct-2 Oct-207 1+ > CD8 4B11 2+ > CD20 L26 3+ > > So CD20 was unaffected but this process affected most of the antigens with > some losing antigen recognition by the antibody (eg CD4 and BOB-1). > > Another one of those pre-analytical issues we need to consider. > > And yes I am writing this up for submission! > > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) | > Principal Scientist; Adjunct Fellow, School of Medicine, University of > Western Sydney; Visiting Lecturer, School of Life Sciences, Faculty of > Science, University of Technology Sydney | Histopathology > t: (02) 9845 3306 | f: (02) 9845 3318 | e: tony.henwood at health.nsw.gov.au > | w: www.schn.health.nsw.gov.au > m: > > > Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia > Locked Bag 4001, Westmead 2145, NSW Australia > > ? Please consider the environment before printing this email. > > -----Original Message----- > From: Greg Dobbin via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, 8 May 2019 5:07 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Processing Schedule- ASP-6025 > > Hello colleagues, > I recently stained (IHC) a section of normal tonsil from another facility > with p16 and the resulting stain was better than the same stain on a > section of my labs own normal tonsil control. > > This has led us to question our processing schedule. I am not concerned > with our fixation because we fix everything for at least 24 hours in 10% > formalin (commercially prepared) prior to processing. > > Does anything jump out at you as being a potential red flag in the > following overnight protocol? > > - Formalin 15 mins; RT > - Processing water 1 min; RT > - ETOH 70% 30 mins; 35C > - ETOH 80% 30 mins; 35C > - ETOH 95% 30 mins; 35C > - ETOH 100% 30 mins; 35C > - ETOH 100% 40 mins; 35C > - ETOH 100% 60 mins; 35C > - Xylene 60 mins; 35C > - Xylene 60 mins; 35C > - Xylene 60 mins; 35C > - Paraffin 60 mins; 57C; vacuum > - Paraffin 60 mins; 57C; vacuum > - Paraffin 60 mins; 57C; vacuum > > Our formalin is changed after every 1100 cassettes and the alcohol, > xylenes and paraffins are managed similarly by the instrument. Our specimen > mix is a little of everything (skins, GIs, breasts, needle cores, gall > bladders, gyne, etc). > > The one unknown (so far) in this story, is how the tonsil from the other > laboratory was handled (ie the fixative used and for how long-I am assuming > 10% formalin). > > Obviously, many of you will have schedules that differ from this one, in > any number of ways, but what I am looking for from you is your opinion: *is > there anything about this schedule that is particularly concerning?* Thank > you, Greg > > > -- > *Greg Dobbin* > 1205 Pleasant Grove Rd > > RR#2 York, > PE C0A 1P0 > > > *Everything in moderation...even moderation itself**!* > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain > confidential information. If you are not the intended recipient, please > delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and > are not necessarily the views of NSW Health or any of its entities. > -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From john.garratt at ciqc.ca Wed May 8 08:50:02 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Wed, 08 May 2019 13:50:02 +0000 Subject: [Histonet] Processing Schedule- ASP-6025 In-Reply-To: References: <665e381823e244b7b6385deeb9dfaa45@UNDCMBX-MEX023.nswhealth.net> Message-ID: The processing schedule looks fine. I am thinking that if you are having no problems with morphology on the H&E processing is not the issue. The extended period in the warming draw is a good hypothesis. I do have a question. How long was that particular piece of tonsil kept in formalin before processing? Could over fixation be the issue? I have found that maintaining tight control of control tissues is important which includes minimum and maximum fixation time. John Sent from ProtonMail Mobile On Tue, May 7, 2019 at 5:52 PM, Greg Dobbin via Histonet wrote: > Fascinating Tony! > We don?t typically leave them in the warming drawer any longer than a > couple of hours, but maybe this particular tonsil was left linger for some > reason and no one thought anything of it?! Something to consider for sure! > Thanks. > Greg > > On Tue, May 7, 2019 at 9:37 PM Tony Henwood (SCHN) < > tony.henwood at health.nsw.gov.au> wrote: > >> Processing seems adequate. >> >> After processing, how long do they sit in the embedding centre block >> holding tank before embedding? >> >> We found that quite a few antigens were affected when we stored control >> tonsil in the embedding centre (dry) at 60oC for a few days before >> embedding. In summary: >> >> Antibody Clone Dried (Normal = 3+) >> CD4 4B12 0 >> BOB-1 TG14 0 >> CD3 LN10 1+ >> CD79a JCB117 1+ >> Oct-2 Oct-207 1+ >> CD8 4B11 2+ >> CD20 L26 3+ >> >> So CD20 was unaffected but this process affected most of the antigens with >> some losing antigen recognition by the antibody (eg CD4 and BOB-1). >> >> Another one of those pre-analytical issues we need to consider. >> >> And yes I am writing this up for submission! >> >> >> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) | >> Principal Scientist; Adjunct Fellow, School of Medicine, University of >> Western Sydney; Visiting Lecturer, School of Life Sciences, Faculty of >> Science, University of Technology Sydney | Histopathology >> t: (02) 9845 3306 | f: (02) 9845 3318 | e: tony.henwood at health.nsw.gov.au >> | w: www.schn.health.nsw.gov.au >> m: >> >> >> Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia >> Locked Bag 4001, Westmead 2145, NSW Australia >> >> ? Please consider the environment before printing this email. >> >> -----Original Message----- >> From: Greg Dobbin via Histonet [mailto:histonet at lists.utsouthwestern.edu] >> Sent: Wednesday, 8 May 2019 5:07 AM >> To: histonet at lists.utsouthwestern.edu >> Subject: [Histonet] Processing Schedule- ASP-6025 >> >> Hello colleagues, >> I recently stained (IHC) a section of normal tonsil from another facility >> with p16 and the resulting stain was better than the same stain on a >> section of my labs own normal tonsil control. >> >> This has led us to question our processing schedule. I am not concerned >> with our fixation because we fix everything for at least 24 hours in 10% >> formalin (commercially prepared) prior to processing. >> >> Does anything jump out at you as being a potential red flag in the >> following overnight protocol? >> >> - Formalin 15 mins; RT >> - Processing water 1 min; RT >> - ETOH 70% 30 mins; 35C >> - ETOH 80% 30 mins; 35C >> - ETOH 95% 30 mins; 35C >> - ETOH 100% 30 mins; 35C >> - ETOH 100% 40 mins; 35C >> - ETOH 100% 60 mins; 35C >> - Xylene 60 mins; 35C >> - Xylene 60 mins; 35C >> - Xylene 60 mins; 35C >> - Paraffin 60 mins; 57C; vacuum >> - Paraffin 60 mins; 57C; vacuum >> - Paraffin 60 mins; 57C; vacuum >> >> Our formalin is changed after every 1100 cassettes and the alcohol, >> xylenes and paraffins are managed similarly by the instrument. Our specimen >> mix is a little of everything (skins, GIs, breasts, needle cores, gall >> bladders, gyne, etc). >> >> The one unknown (so far) in this story, is how the tonsil from the other >> laboratory was handled (ie the fixative used and for how long-I am assuming >> 10% formalin). >> >> Obviously, many of you will have schedules that differ from this one, in >> any number of ways, but what I am looking for from you is your opinion: *is >> there anything about this schedule that is particularly concerning?* Thank >> you, Greg >> >> >> -- >> *Greg Dobbin* >> 1205 Pleasant Grove Rd >> >> RR#2 York, >> PE C0A 1P0 >> >> >> *Everything in moderation...even moderation itself**!* >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> This message is intended for the addressee named and may contain >> confidential information. If you are not the intended recipient, please >> delete it and notify the sender. >> >> Views expressed in this message are those of the individual sender, and >> are not necessarily the views of NSW Health or any of its entities. >> > -- > *Greg Dobbin* > 1205 Pleasant Grove Rd > RR#2 York, > PE C0A 1P0 > > *Everything in moderation...even moderation itself**!* > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed May 8 09:31:02 2019 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 8 May 2019 10:31:02 -0400 Subject: [Histonet] 2019 Hot Histology Job Alert! #jobs4myhistopeeps in FL, CA, NC, AZ, TX, WI, VA and TN Message-ID: <00c101d505aa$a9a94680$fcfbd380$@earthlink.net> Hello Histopeeps, I hope you are having a great week. I wanted to send a quick note to tell you about the positions that I am working on and am most excited about.? Why am I excited about these positions? Because each of these clients was asked: ?If I had a histotech for you today would you be ready to interview and hire?? Every One these clients said YES!!! All of these positions are full time and permanent!! My clients offer excellent compensation, benefits and in most cases relocation and/or a sign-on bonus. Histopeeps, I have exciting opportunities in: ? Florida! ? California! ? North Carolina! ? Texas! ? Virginia! ? Wisconsin! ? Tennessee ! If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I place someone you refer You will earn a referral fee. I can be reached toll free at the office at 866-607-3542 or relia1 at earthlink.net Or you can always catch me on cell call/text at 407-353-5070. Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From greg.dobbin at gmail.com Wed May 8 10:59:20 2019 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Wed, 8 May 2019 12:59:20 -0300 Subject: [Histonet] Processing Schedule- ASP-6025 In-Reply-To: References: <665e381823e244b7b6385deeb9dfaa45@UNDCMBX-MEX023.nswhealth.net> Message-ID: Great point John! We do attempt to have all of the specimen handling for our control tissue, match that of our specimens. However, tonsillectomies at our hospital are only performed on Thursdays. So we fix them for 24hrs (until Friday) then process overnight and remove from the processor on Saturday (we don't typically work Saturdays). And I have done this a couple of times but can I swear that this particular tonsil was handled that way? I cannot ...which I know, points to another deficiency! :-0 It could very well be that the fixation times of the two tonsils I am comparing are sufficiently different that they will require different retrieval times to produce stains of similar intensity. I also appreciate getting your opinion on the processing schedule. You have much more experience than I!! Thank you for your insights. All the best, Greg On Wed, May 8, 2019 at 10:50 AM John Garratt wrote: > The processing schedule looks fine. I am thinking that if you are having > no problems with morphology on the H&E processing is not the issue. The > extended period in the warming draw is a good hypothesis. > I do have a question. How long was that particular piece of tonsil kept in > formalin before processing? Could over fixation be the issue? > I have found that maintaining tight control of control tissues is > important which includes minimum and maximum fixation time. > > John > > Sent from ProtonMail Mobile > > > On Tue, May 7, 2019 at 5:52 PM, Greg Dobbin via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > Fascinating Tony! > We don?t typically leave them in the warming drawer any longer than a > couple of hours, but maybe this particular tonsil was left linger for some > reason and no one thought anything of it?! Something to consider for sure! > Thanks. > Greg > > On Tue, May 7, 2019 at 9:37 PM Tony Henwood (SCHN) < > tony.henwood at health.nsw.gov.au> wrote: > > > Processing seems adequate. > > > > After processing, how long do they sit in the embedding centre block > > holding tank before embedding? > > > > We found that quite a few antigens were affected when we stored control > > tonsil in the embedding centre (dry) at 60oC for a few days before > > embedding. In summary: > > > > Antibody Clone Dried (Normal = 3+) > > CD4 4B12 0 > > BOB-1 TG14 0 > > CD3 LN10 1+ > > CD79a JCB117 1+ > > Oct-2 Oct-207 1+ > > CD8 4B11 2+ > > CD20 L26 3+ > > > > So CD20 was unaffected but this process affected most of the antigens > with > > some losing antigen recognition by the antibody (eg CD4 and BOB-1). > > > > Another one of those pre-analytical issues we need to consider. > > > > And yes I am writing this up for submission! > > > > > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) | > > Principal Scientist; Adjunct Fellow, School of Medicine, University of > > Western Sydney; Visiting Lecturer, School of Life Sciences, Faculty of > > Science, University of Technology Sydney | Histopathology > > t: (02) 9845 3306 | f: (02) 9845 3318 | e: > tony.henwood at health.nsw.gov.au > > | w: www.schn.health.nsw.gov.au > > m: > > > > > > Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia > > Locked Bag 4001, Westmead 2145, NSW Australia > > > > ? Please consider the environment before printing this email. > > > > -----Original Message----- > > From: Greg Dobbin via Histonet [mailto:histonet at lists.utsouthwestern.edu > ] > > Sent: Wednesday, 8 May 2019 5:07 AM > > To: histonet at lists.utsouthwestern.edu > > Subject: [Histonet] Processing Schedule- ASP-6025 > > > > Hello colleagues, > > I recently stained (IHC) a section of normal tonsil from another facility > > with p16 and the resulting stain was better than the same stain on a > > section of my labs own normal tonsil control. > > > > This has led us to question our processing schedule. I am not concerned > > with our fixation because we fix everything for at least 24 hours in 10% > > formalin (commercially prepared) prior to processing. > > > > Does anything jump out at you as being a potential red flag in the > > following overnight protocol? > > > > - Formalin 15 mins; RT > > - Processing water 1 min; RT > > - ETOH 70% 30 mins; 35C > > - ETOH 80% 30 mins; 35C > > - ETOH 95% 30 mins; 35C > > - ETOH 100% 30 mins; 35C > > - ETOH 100% 40 mins; 35C > > - ETOH 100% 60 mins; 35C > > - Xylene 60 mins; 35C > > - Xylene 60 mins; 35C > > - Xylene 60 mins; 35C > > - Paraffin 60 mins; 57C; vacuum > > - Paraffin 60 mins; 57C; vacuum > > - Paraffin 60 mins; 57C; vacuum > > > > Our formalin is changed after every 1100 cassettes and the alcohol, > > xylenes and paraffins are managed similarly by the instrument. Our > specimen > > mix is a little of everything (skins, GIs, breasts, needle cores, gall > > bladders, gyne, etc). > > > > The one unknown (so far) in this story, is how the tonsil from the other > > laboratory was handled (ie the fixative used and for how long-I am > assuming > > 10% formalin). > > > > Obviously, many of you will have schedules that differ from this one, in > > any number of ways, but what I am looking for from you is your opinion: > *is > > there anything about this schedule that is particularly concerning?* > Thank > > you, Greg > > > > > > -- > > *Greg Dobbin* > > 1205 Pleasant Grove Rd > > > > RR#2 York, > > PE C0A 1P0 > > > > > > *Everything in moderation...even moderation itself**!* > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > This message is intended for the addressee named and may contain > > confidential information. If you are not the intended recipient, please > > delete it and notify the sender. > > > > Views expressed in this message are those of the individual sender, and > > are not necessarily the views of NSW Health or any of its entities. > > > -- > *Greg Dobbin* > 1205 Pleasant Grove Rd > RR#2 York, > PE C0A 1P0 > > > *Everything in moderation...even moderation itself**!* > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From cls71877 at gmail.com Wed May 8 15:20:24 2019 From: cls71877 at gmail.com (Cristi Rigazio) Date: Wed, 8 May 2019 16:20:24 -0400 Subject: [Histonet] Benchmarks for Reprocessing References: Message-ID: <8C3B3B9E-6878-4932-9E20-4C1700840F6E@gmail.com> > Good afternoon Fellow histonetters! > > I was wondering if there are any tertiary institutions out there that have set a benchmark for reprocessing tissue? We tend to be less than 1%, but would love to see what others think is reasonable. > > Thanks for the feedback and hope you all are having an excellent day! > Cristi From Timothy.Morken at ucsf.edu Wed May 8 15:39:08 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 8 May 2019 20:39:08 +0000 Subject: [Histonet] Benchmarks for Reprocessing In-Reply-To: <8C3B3B9E-6878-4932-9E20-4C1700840F6E@gmail.com> References: <8C3B3B9E-6878-4932-9E20-4C1700840F6E@gmail.com> Message-ID: We try to be at 0.5% or less. We monitored as a quality indicator for several years and had to modify something to get to that point, but it is pretty steady now. We have new residents constantly rotating into pathology so it is a training issue every month. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cristi Rigazio via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, May 08, 2019 1:20 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Benchmarks for Reprocessing > Good afternoon Fellow histonetters! > > I was wondering if there are any tertiary institutions out there that have set a benchmark for reprocessing tissue? We tend to be less than 1%, but would love to see what others think is reasonable. > > Thanks for the feedback and hope you all are having an excellent day! > Cristi _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jcox90 at yahoo.com Thu May 9 09:43:24 2019 From: Jcox90 at yahoo.com (Jill Cox) Date: Thu, 9 May 2019 07:43:24 -0700 Subject: [Histonet] Part time histology in Phoenix area? Message-ID: <3A38609B-E47E-44E7-A02D-5CC0157F25D6@yahoo.com> Hello all, Are there any part time or on call openings in the Phoenix area for histology? Thanks!!! Sent from my iPhone From tbraud at holyredeemer.com Thu May 9 12:33:17 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 9 May 2019 17:33:17 +0000 Subject: [Histonet] Benchmarks for reprocessing Message-ID: <48E053DDF6CE074DB6A7414BA05403F8015706F5D6@HRHEX02-HOS.holyredeemer.local> We are down to < 0.1%, but I think you have to take into account your grossing staff. We are just medium sized and have an outstanding PA. Our 2 new Leica 6025 processors have also made a big difference. I suspect if you are a teaching hospital with residents grossing, there would be a learning curve. When I was at UVA, our percentages were not so good until we hired a PA to oversee the gross room and to teach grossing skills. Just my 2 cents. Thx, T Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, May 09, 2019 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 186, Issue 8 CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links or opening attachments. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Benchmarks for Reprocessing (Cristi Rigazio) 2. Re: Benchmarks for Reprocessing (Morken, Timothy) 3. Part time histology in Phoenix area? (Jill Cox) ---------------------------------------------------------------------- Message: 1 Date: Wed, 8 May 2019 16:20:24 -0400 From: Cristi Rigazio To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Benchmarks for Reprocessing Message-ID: <8C3B3B9E-6878-4932-9E20-4C1700840F6E at gmail.com> Content-Type: text/plain; charset=us-ascii > Good afternoon Fellow histonetters! > > I was wondering if there are any tertiary institutions out there that have set a benchmark for reprocessing tissue? We tend to be less than 1%, but would love to see what others think is reasonable. > > Thanks for the feedback and hope you all are having an excellent day! > Cristi ------------------------------ Message: 2 Date: Wed, 8 May 2019 20:39:08 +0000 From: "Morken, Timothy" To: Cristi Rigazio Cc: Histonet Subject: Re: [Histonet] Benchmarks for Reprocessing Message-ID: Content-Type: text/plain; charset="us-ascii" We try to be at 0.5% or less. We monitored as a quality indicator for several years and had to modify something to get to that point, but it is pretty steady now. We have new residents constantly rotating into pathology so it is a training issue every month. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cristi Rigazio via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, May 08, 2019 1:20 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Benchmarks for Reprocessing > Good afternoon Fellow histonetters! > > I was wondering if there are any tertiary institutions out there that have set a benchmark for reprocessing tissue? We tend to be less than 1%, but would love to see what others think is reasonable. > > Thanks for the feedback and hope you all are having an excellent day! > Cristi _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Thu, 9 May 2019 07:43:24 -0700 From: Jill Cox To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Part time histology in Phoenix area? Message-ID: <3A38609B-E47E-44E7-A02D-5CC0157F25D6 at yahoo.com> Content-Type: text/plain; charset=us-ascii Hello all, Are there any part time or on call openings in the Phoenix area for histology? Thanks!!! Sent from my iPhone ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 186, Issue 8 **************************************** From rsrichmond at gmail.com Thu May 9 15:26:37 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 9 May 2019 16:26:37 -0400 Subject: [Histonet] Benchmarks for reprocessing In-Reply-To: References: Message-ID: Cristi Rigazio asks: I was wondering if there are any tertiary institutions out there that have set a benchmark for reprocessing tissue? We tend to be less than 1%, but would love to see what others think is reasonable.<< This grumpy old pathologist sez: If I'm the one doing the grossing, I don't expect to see any reprocessing. I can't remember having had a block reprocessed, or having one that needed it after I learned how to gross around 50 years ago. Make your pathologists cut it right! Bob Richmond Samurai Pathologist Maryville TN From tony.auge at gmail.com Fri May 10 11:46:25 2019 From: tony.auge at gmail.com (Tony Auge) Date: Fri, 10 May 2019 09:46:25 -0700 Subject: [Histonet] Processing Schedule- ASP-6025 In-Reply-To: References: <665e381823e244b7b6385deeb9dfaa45@UNDCMBX-MEX023.nswhealth.net> Message-ID: What are you using for your lab's own control. Was that tissue processed recently? Do you have normal tonsil that you process in-house regularly that also stains poorly? On Wed, May 8, 2019 at 9:16 AM Greg Dobbin via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Great point John! > We do attempt to have all of the specimen handling for our control tissue, > match that of our specimens. However, > tonsillectomies at our hospital are only performed on Thursdays. So we fix > them for 24hrs (until Friday) then process overnight and remove > from the processor on Saturday (we don't typically work Saturdays). And I > have done this a couple of times but can I swear that this particular > tonsil > was handled that way? I cannot ...which I know, points to another > deficiency! :-0 It could very well be that the fixation times of the two > tonsils I am comparing are sufficiently different that they will require > different retrieval times to produce stains of similar intensity. > > I also appreciate getting your opinion on the processing schedule. You have > much more experience than I!! Thank you for your insights. > All the best, > Greg > > On Wed, May 8, 2019 at 10:50 AM John Garratt wrote: > > > The processing schedule looks fine. I am thinking that if you are having > > no problems with morphology on the H&E processing is not the issue. The > > extended period in the warming draw is a good hypothesis. > > I do have a question. How long was that particular piece of tonsil kept > in > > formalin before processing? Could over fixation be the issue? > > I have found that maintaining tight control of control tissues is > > important which includes minimum and maximum fixation time. > > > > John > > > > Sent from ProtonMail Mobile > > > > > > On Tue, May 7, 2019 at 5:52 PM, Greg Dobbin via Histonet < > > histonet at lists.utsouthwestern.edu> wrote: > > > > Fascinating Tony! > > We don?t typically leave them in the warming drawer any longer than a > > couple of hours, but maybe this particular tonsil was left linger for > some > > reason and no one thought anything of it?! Something to consider for > sure! > > Thanks. > > Greg > > > > On Tue, May 7, 2019 at 9:37 PM Tony Henwood (SCHN) < > > tony.henwood at health.nsw.gov.au> wrote: > > > > > Processing seems adequate. > > > > > > After processing, how long do they sit in the embedding centre block > > > holding tank before embedding? > > > > > > We found that quite a few antigens were affected when we stored control > > > tonsil in the embedding centre (dry) at 60oC for a few days before > > > embedding. In summary: > > > > > > Antibody Clone Dried (Normal = 3+) > > > CD4 4B12 0 > > > BOB-1 TG14 0 > > > CD3 LN10 1+ > > > CD79a JCB117 1+ > > > Oct-2 Oct-207 1+ > > > CD8 4B11 2+ > > > CD20 L26 3+ > > > > > > So CD20 was unaffected but this process affected most of the antigens > > with > > > some losing antigen recognition by the antibody (eg CD4 and BOB-1). > > > > > > Another one of those pre-analytical issues we need to consider. > > > > > > And yes I am writing this up for submission! > > > > > > > > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) | > > > Principal Scientist; Adjunct Fellow, School of Medicine, University of > > > Western Sydney; Visiting Lecturer, School of Life Sciences, Faculty of > > > Science, University of Technology Sydney | Histopathology > > > t: (02) 9845 3306 | f: (02) 9845 3318 | e: > > tony.henwood at health.nsw.gov.au > > > | w: www.schn.health.nsw.gov.au > > > m: > > > > > > > > > Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia > > > Locked Bag 4001, Westmead 2145, NSW Australia > > > > > > ? Please consider the environment before printing this email. > > > > > > -----Original Message----- > > > From: Greg Dobbin via Histonet [mailto: > histonet at lists.utsouthwestern.edu > > ] > > > Sent: Wednesday, 8 May 2019 5:07 AM > > > To: histonet at lists.utsouthwestern.edu > > > Subject: [Histonet] Processing Schedule- ASP-6025 > > > > > > Hello colleagues, > > > I recently stained (IHC) a section of normal tonsil from another > facility > > > with p16 and the resulting stain was better than the same stain on a > > > section of my labs own normal tonsil control. > > > > > > This has led us to question our processing schedule. I am not concerned > > > with our fixation because we fix everything for at least 24 hours in > 10% > > > formalin (commercially prepared) prior to processing. > > > > > > Does anything jump out at you as being a potential red flag in the > > > following overnight protocol? > > > > > > - Formalin 15 mins; RT > > > - Processing water 1 min; RT > > > - ETOH 70% 30 mins; 35C > > > - ETOH 80% 30 mins; 35C > > > - ETOH 95% 30 mins; 35C > > > - ETOH 100% 30 mins; 35C > > > - ETOH 100% 40 mins; 35C > > > - ETOH 100% 60 mins; 35C > > > - Xylene 60 mins; 35C > > > - Xylene 60 mins; 35C > > > - Xylene 60 mins; 35C > > > - Paraffin 60 mins; 57C; vacuum > > > - Paraffin 60 mins; 57C; vacuum > > > - Paraffin 60 mins; 57C; vacuum > > > > > > Our formalin is changed after every 1100 cassettes and the alcohol, > > > xylenes and paraffins are managed similarly by the instrument. Our > > specimen > > > mix is a little of everything (skins, GIs, breasts, needle cores, gall > > > bladders, gyne, etc). > > > > > > The one unknown (so far) in this story, is how the tonsil from the > other > > > laboratory was handled (ie the fixative used and for how long-I am > > assuming > > > 10% formalin). > > > > > > Obviously, many of you will have schedules that differ from this one, > in > > > any number of ways, but what I am looking for from you is your opinion: > > *is > > > there anything about this schedule that is particularly concerning?* > > Thank > > > you, Greg > > > > > > > > > -- > > > *Greg Dobbin* > > > 1205 Pleasant Grove Rd > > > < > https://maps.google.com/?q=1205+Pleasant+Grove+Rd&entry=gmail&source=g> > > > RR#2 York, > > > PE C0A 1P0 > > > > > > > > > *Everything in moderation...even moderation itself**!* > > > _______________________________________________ > > > Histonet mailing list > > > Histonet at lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > This message is intended for the addressee named and may contain > > > confidential information. If you are not the intended recipient, please > > > delete it and notify the sender. > > > > > > Views expressed in this message are those of the individual sender, and > > > are not necessarily the views of NSW Health or any of its entities. > > > > > -- > > *Greg Dobbin* > > 1205 Pleasant Grove Rd > > RR#2 York, > > PE C0A 1P0 > > > > > > *Everything in moderation...even moderation itself**!* > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > -- > *Greg Dobbin* > 1205 Pleasant Grove Rd > RR#2 York, > PE C0A 1P0 > > > *Everything in moderation...even moderation itself**!* > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tony Auge HTL (ASCP)CM QIHC Cell: (651) 373-4768 Email: tony.auge at gmail.com From Nancy_Schmitt at pa-ucl.com Fri May 10 14:55:13 2019 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Fri, 10 May 2019 19:55:13 +0000 Subject: [Histonet] waste removal Message-ID: <0945c5b957c547458c6fc5f2a9e6c0d8@pa-ucl.com> Happy Friday! Is your waste incinerated/autoclaved or some of both? Is anyone having to marker over PHI on specimen containers before being removed? Interested in processes and what is working best. Thanks Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From katherine at ka-recruiting.com Fri May 10 15:30:49 2019 From: katherine at ka-recruiting.com (Katherine Marano) Date: Fri, 10 May 2019 16:30:49 -0400 Subject: [Histonet] new histotech opening in florida Message-ID: Hi Histonetters, Happy Friday! I hope you are looking forward to a nice weekend. I wanted to reach out to you about a new Histotechnologist position in the Jacksonville, Florida area. It will probably be a very early morning shift, but not sure exactly what the hours will be yet. Monday ? Friday, 40 hour work week. Must be ASCP certified and have a Florida State license. Would like someone with grossing experience. This is a full-time and permanent positions with fantastic compensations and benefits. If you are interested, could you send me a resume and a good time for us to get in touch? If not, do you know of anyone who may be a good fit? Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From relia1 at earthlink.net Tue May 14 10:16:34 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 14 May 2019 11:16:34 -0400 Subject: [Histonet] Having an Amazing Week? I hope so!! Check out my current bulletin for some exciting new opportunities on and off the bench!! Message-ID: <006801d50a68$04c82600$0e587200$@earthlink.net> Hi Histopeeps! I hope you had a lovely Mother?s Day!!! Mine was nice and quiet and I really needed it!!!! WHY? Because, my phone has been ringing off the hook this past week with some exciting new opportunities!!! This is a quick update of the positions I am working on that I am most excited about. Some are brand new and some are additional positions where I have placed people who LOVE their jobs. As you know all of the positions I work with are full-time permanent positions with the best hospitals, labs and clinics. I only work with clients that offer excellent compensation including competitive salaries, great benefits and relocation assistance /sign on bonuses. Spotlight Opportunities: IHC All day EVERY DAY!!! IHC Specialist ? Stockton, CA IHC Specialist ? Chicago area IHC Specialist ? Southern CA IHC Specialist ? Phoenix, AZ Mentor the future GEN of Histopeeps!! Histology Program Instructor needed in Virginia!! Get into Histology Sales! Territory Sales Manager ? Ohio Territory Sales Manager ? New England Here is a list of the tech positions I am excited to tell you about: Houston, TX Lead Histology Tech A RELIA Exclusive! Stockton, CA Histology Tech ASCP elig &New Grads welcome! Norfolk, VA Lead Histotech Oppty to GROW!!! Nashville, TN Histotech CLIA qualified to gross a plus! North Carolina HT/HTL several locations in NC! Panama City, FL Histotech Will help with FL license!! Chattanooga, TN Histotech CLIA qualified to gross a plus! Milwaukee, WI Histotech Dayshift sign on bonus!! Histopeeps, if you are interested in any of these opportunities contact me ASAP!!!! If you are ready to move my clients are too!!! Call/text 407-353-5070. Or toll free at the office at 866-607-3542 Or e-mail me at relia1 at earthlink.net with your resume and a number where I can reach you at a time that is convenient for you. Not Exactly what you?re looking for? No Worries!! Shoot me a quick email and let me know what YOU are looking for! I have clients nationwide and I will keep your resume confidential. I will only represent you to jobs you want me to represent you to. Remember It never hurts to keep an eye open even if you are happy in your present job. Also Histopeeps, if you know anyone else that might be interested I would really appreciate it if you would pass my information along to them as well. If I place someone you refer you will earn a referral fee! Maybe even that traveler in your lab is ready to settle back down all you have to do is refer them to me and if I place them you will earn a referral fee!! Again, I hope you had a wonderful Mother?s Day and that it is the start to an amazing week for you!!! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From pslath at gwu.edu Tue May 14 18:54:30 2019 From: pslath at gwu.edu (Patricia Latham) Date: Tue, 14 May 2019 19:54:30 -0400 Subject: [Histonet] Histonet listserve Message-ID: Hello to users of the Histonet listserve. I would like to let the community know that there is a position available in the Research Pathology Core Lab at George Washington University School of Medicine in Washington, DC. I am providing the following job description. Feel free to contact me directly by email with any questions you may have, but I also encourage you to apply on-line, if you are interested, at GWJobs - Lab Associate. Pat Latham, MD - Lab Director *Full-time Research Assistant ? Histotechnologist and Laboratory Manager* A position is available for a histotechnologist for a Research Pathology Core Lab in the *GWU School of Medicine and Health Sciences* in Washington, DC. This person will be expected to maintain a histology laboratory, including ordering of supplies, washing glassware and maintaining equipment for optimal function, daily logs with inventory of specimens and blocks, labeling of samples and slides, tissue processing and embedding, microtome and cryostat sections, routine stains, special stains and immunostains with appropriate controls, as required to meet the needs of researchers at GWU. This person will need to work independently to deliver excellent quality results in a timely manner. The person should have strong communication skills and managerial skills, since there will be a need to work with researchers in several labs and to maintain inventory from several sources, to keep track of the flow of specimens and charges. The position will require data entry into an audit-trail database and the maintenance of meticulous records. This person should have some experience in laboratory research, implementation of new techniques and the ability to trouble-shoot problems that might arise in this setting. *Entry level qualifications:* ? Bachelor?s degree in a clinical science with a course in Histology or equivalent combination of training and experience. ? Experience working in a research setting. ? Eligible (with certification pending) or certified as a Histotechnologist or Histotechnician by the American Society for Clinical Pathologists. From lkc0001 at auburn.edu Wed May 15 10:20:34 2019 From: lkc0001 at auburn.edu (Lisa Parsons) Date: Wed, 15 May 2019 15:20:34 +0000 Subject: [Histonet] Chromogranin A in dogs Message-ID: Our CgA has stopped working (IHC/P). Does anyone have a source for a CgA that works in dogs? From sandra.cheasty at wisc.edu Wed May 15 10:53:14 2019 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Wed, 15 May 2019 15:53:14 +0000 Subject: [Histonet] Thyroglobulin TGB04+TGB05 Not Working in Canine and Feline Message-ID: Hello, We had to switch sources (to Novus) for Thyroglobulin TGB04+TGB05 and cannot get it to work in canines or felines. Data sheet suggests 1:100, pH6 citrate antigen retrieval. We've also tried zero AR and pH9 EDTA AR, to no avail. Any suggestions? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From shive003 at umn.edu Wed May 15 12:51:45 2019 From: shive003 at umn.edu (Jan Shivers) Date: Wed, 15 May 2019 12:51:45 -0500 Subject: [Histonet] Chromogranin A in dogs In-Reply-To: References: Message-ID: Hi Lisa, I just worked up an antibody from abcam; cat. # ab85554 Rabbit polyclonal 1:1200 (0.6ug/ml) -1:1600 (0.4 ug/ml) Heat retrieval in pH 6.0 solution 45 min primary Ab, 45 min secondary Ab (polymer system), 15 min chromogen (AEC+) Regards, Jan Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003 at umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Wed, May 15, 2019 at 10:20 AM Lisa Parsons via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Our CgA has stopped working (IHC/P). Does anyone have a source for a CgA > that works in dogs? > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From shive003 at umn.edu Wed May 15 12:58:05 2019 From: shive003 at umn.edu (Jan Shivers) Date: Wed, 15 May 2019 12:58:05 -0500 Subject: [Histonet] Thyroglobulin TGB04+TGB05 Not Working in Canine and Feline In-Reply-To: References: Message-ID: Hi Sandra, Being a mouse monoclonal, it may not cross-react with canines/felines; the epitope may be specific to human follicular cells only. The polyclonal Thyroglobulin from Dako/Agilent works fine on all the mammals I've tried it on so far (and a frog as well). Regards, Jan Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003 at umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Wed, May 15, 2019 at 10:54 AM Sandra Cheasty via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello, > We had to switch sources (to Novus) for Thyroglobulin > TGB04+TGB05 and cannot get it to work in canines or felines. Data sheet > suggests 1:100, pH6 citrate antigen retrieval. We've also tried zero AR and > pH9 EDTA AR, to no avail. Any suggestions? > Thanks! > Sandy > > Sandra J. Cheasty, HT (ASCP) > Histology & Necropsy Supervisor > UW-Madison, School of Veterinary Medicine > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From greg.dobbin at gmail.com Wed May 15 13:18:06 2019 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Wed, 15 May 2019 15:18:06 -0300 Subject: [Histonet] Thyroglobulin TGB04+TGB05 Not Working in Canine and Feline Message-ID: Hi Sandra, >From your post, it looks to me like you are assuming the recommended dilution should work. I apologize in advance if I have misinterpreted your post in this regard. When starting with a new antibody (including a new vendor for same Ab and/or a new clone), do a range of dilutions that includes the recommended one. So in this case, I would try 1:50, 1:100 and 1:200 to start. Use the recommended AR first and then see how each of the 3 dilutions look. Just as an example, you may find 1:50 has a weak signal and higher dilutions are negative. If so, set up 3 more dilutions with the same AR (eg 1:10, 1:25 and 1:50). Once you get close to where you think the intensity should be you can try modifying the AR protocol to improve the sensitivity. Good luck, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From relia1 at earthlink.net Mon May 20 10:19:27 2019 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 20 May 2019 11:19:27 -0400 Subject: [Histonet] IHC Specialists Needed CA, IL, AZ and New England. Message-ID: <00af01d50f1f$6a6626e0$3f3274a0$@earthlink.net> Hi Histonetters! Just a quick note about some exciting new opportunities. I am recruiting for IHC Specialists nationwide. I have a number of roles ranging from in-house to field applications specialists. If you are interested please shoot me a quick email for details. And. If you refer someone that I place you will earn a referral fee. Thanks-Pam To subscribe to my histology careers bulletin reply to this email with subscribe. #jobs4myhistopeeps #ilovemyhistopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From sandra.cheasty at wisc.edu Mon May 20 11:09:43 2019 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Mon, 20 May 2019 16:09:43 +0000 Subject: [Histonet] Motorized Microtome Opinions Message-ID: Hello all, Our only motorized Microm microtome is slowly dying, and I'm looking for a replacement that can be used manually, semi, or fully automatic. I'm leaning towards Leica as I have had good luck with their manual microtomes. Please, experienced histotech comments only, no sales pitches... Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From relia1 at earthlink.net Tue May 21 08:52:39 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 21 May 2019 09:52:39 -0400 Subject: [Histonet] RELIA Histology Job Alert Message-ID: <000001d50fdc$74798380$5d6c8a80$@earthlink.net> Hi Histopeeps! I hope you are having a great day. Here is a list of my current openings: Management: Indiana Pennsylvania Chicago Texas Arizona Tech positions: California North Carolina Florida Wisconsin Tennessee Sales and Support: Virginia Arizona Ohio New Jersey All of these positions are full time permanent positions and my clients offer great compensation packages most of which include relocation/sign on bonuses. Out of respect to the histonet I will be limiting my posts to the basic information on the positions I am representing. If you would like to see all of the details please subscribe to my Career News Bulletin by replying to this post with ?Subscribe? Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From sandra.cheasty at wisc.edu Tue May 21 11:57:16 2019 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Tue, 21 May 2019 16:57:16 +0000 Subject: [Histonet] Galileo Microtome Message-ID: Hello all, Has anyone had experience using the Galileo Series 2 microtome, made by Diapath? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From Joanna.Bartczak at uhn.ca Tue May 21 14:16:08 2019 From: Joanna.Bartczak at uhn.ca (Bartczak, Joanna) Date: Tue, 21 May 2019 19:16:08 +0000 Subject: [Histonet] TMArrayer: Pathology Devices In-Reply-To: References: Message-ID: Hello, I am looking to purchase new punches for the TMArrayer and am not having any luck contacting anyone at Pathology Devices (Ron). Does anyone out there in Histoland have current contact information? Help! Thanks in advance, Joanna Joanna Bartczak Charge Technologist - Immunopathology University Health Network - Toronto General Site 200 Elizabeth Street Toronto, Ontario M5G 2C4 Phone: (416) 340-4800 ext.8037 Joanna.Bartczak at uhn.ca This e-mail may contain confidential and/or privileged information for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. Opinions, conclusions or other information contained in this e-mail may not be that of the organization. If you feel you have received an email from UHN of a commercial nature and would like to be removed from the sender's mailing list please do one of the following: (1) Follow any unsubscribe process the sender has included in their email (2) Where no unsubscribe process has been included, reply to the sender and type "unsubscribe" in the subject line. If you require additional information please go to our UHN Newsletters and Mailing Lists page. Please note that we are unable to automatically unsubscribe individuals from all UHN mailing lists. From pslath at gwu.edu Tue May 21 16:43:56 2019 From: pslath at gwu.edu (Patricia Latham) Date: Tue, 21 May 2019 17:43:56 -0400 Subject: [Histonet] Histotech job opening Message-ID: Hello Histonet users, I would like to make you aware of a job opportunity, You can apply at https://www.gwu.jobs and look for Research and Post Doctoral Scientist /Laboratory Associate, Pathology Core Lab or feel free to call me for more info (202-994-5057) Patricia Latham, Lab Director *Histotechnology* A wage account position is immediately available for a person with skills in histotechnology in a research pathology core laboratory in the GWU School of Biomedical Sciences. This person will be expected to work independently to assist in maintaining a histology laboratory, including ordering of supplies, washing glassware and maintaining equipment for optimal function, daily logs with inventory of specimens and blocks, labeling of samples and slides, tissue processing and embedding, microtome and cryostat sections, routine stains, special stains and immunostains with appropriate controls, as required to meet the needs of researchers at GWU. Experience in laboratory research preferred. *Entry level qualifications:* ? Bachelor?s degree in a clinical science with a course in Histology or equivalent combination of training and experience. ? Experience as a Histotechnologist ? Preferred eligibility or certification as a or Histotechnician ? Preferred experience working in a research setting. From relia1 at earthlink.net Wed May 22 09:58:09 2019 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 22 May 2019 10:58:09 -0400 Subject: [Histonet] Free Resume/CV Critique/tune up - Because Resumes are not just for job-hunting anymore. Message-ID: <00a001d510ae$c54a1c20$4fde5460$@earthlink.net> Hi Histonetters, Since, February 2019 I have been doing free resume tune ups for histotechs. I thought it would be a good idea to offer the same free service to members of the histonet. I have found that it is important to keep your CV/Resume updated for many reasons besides job hunting. For example: It is a record of your accomplishments that can be useful when applying for a promotion at work, to be submitted with a written article or a workshop/presentation. And is a great way for you to map your own career progress. If you would like some help with a Resume or CV just shoot me an email at relia1 at earthlink.net Have a Great Day! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From katherine at ka-recruiting.com Fri May 24 10:39:27 2019 From: katherine at ka-recruiting.com (Katherine Marano) Date: Fri, 24 May 2019 11:39:27 -0400 Subject: [Histonet] new histotech opening in ohio Message-ID: Happy Friday Histonetters! I am working with a client that is looking to hire a full-time Histotech in Cleveland, Ohio. The position is 1st shift, still figuring out the start time but will be 6 or 6:30am. Must be certified and have experience embedding, cutting, and grossing. If you are interested, could you send me a resume and a good time for us to get in touch? If not, do you know of anyone who may be a good fit? Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From Jennifer.J.Schumacher at HealthPartners.Com Tue May 28 12:10:11 2019 From: Jennifer.J.Schumacher at HealthPartners.Com (Schumacher, Jennifer J) Date: Tue, 28 May 2019 17:10:11 +0000 Subject: [Histonet] Use of Freeze Spray in a Cryostat Message-ID: Hi all, Looking for some documentation regarding the use of Freeze Spray in cryostats. A Best Practice document would be ideal, but in the absence of such a document, I would like to hear how others are planning to address the "recall" by Leica where they do not recommend the use of the Freeze Spray in their cryostats due to the possibility of a fire (flammable reagent). I personally have always had concerns around infection since the specimens are fresh and using the spray could aerosolize any infectious agents that are present. There is a lot of pressure to freeze things rapidly to meet the FS TAT, so the battle goes back and forth. Any feedback is much appreciated. Jennifer ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From erin.mccarthy at tempus.com Tue May 28 12:19:32 2019 From: erin.mccarthy at tempus.com (Erin McCarthy) Date: Tue, 28 May 2019 12:19:32 -0500 Subject: [Histonet] Use of Freeze Spray in a Cryostat In-Reply-To: References: Message-ID: Hi Jennifer - It was my understanding that the recall was particularly related to the flammability of the freeze spray. If you can find a spray with out a flammability hazard you can keep using the spray. I don't have a lot of frozen sections in my current job. But in my last job, and my experience in hospitals to get rapid results for patients still on the table sprays are used. In cases of TB they are discouraged. On Tue, May 28, 2019 at 12:12 PM Schumacher, Jennifer J via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all, > Looking for some documentation regarding the use of Freeze Spray in > cryostats. > > A Best Practice document would be ideal, but in the absence of such a > document, I would like to hear how others are planning to address the > "recall" by Leica where they do not recommend the use of the Freeze Spray > in their cryostats due to the possibility of a fire (flammable reagent). > > I personally have always had concerns around infection since the specimens > are fresh and using the spray could aerosolize any infectious agents that > are present. > > There is a lot of pressure to freeze things rapidly to meet the FS TAT, so > the battle goes back and forth. > > Any feedback is much appreciated. > > Jennifer > > > ________________________________ > > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this communication in error, please return it to the > sender immediately and delete the original message and any copy of it from > your computer system. If you have any questions concerning this message, > please contact the sender. Disclaimer R001.0 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Erin McCarthy, HT (ASCP) Histology Supervisor Tempus Labs 600 W. Chicago Ave. Chicago IL 60654 Ph:(312) 638-6344 Ext.3835 -- This email and any attachments may contain privileged and confidential information and/or protected health information (PHI) that is protected by federal and state privacy laws.? It is intended solely for the use of Tempus Labs and the recipient(s) named above.? Nothing contained in this communication and any attachments thereto is intended to waive any privileges or rights of confidentiality.? If you are not the recipient, or the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any review, dissemination, distribution, printing or copying of this email message and/or any attachments is strictly prohibited.?* If you have received this transmission in error, please notify us immediately at?**(855)-442-8305**? and permanently delete this email and any attachments*. From bakevictoria at gmail.com Tue May 28 13:43:38 2019 From: bakevictoria at gmail.com (Victoria Baker) Date: Tue, 28 May 2019 14:43:38 -0400 Subject: [Histonet] Use of Freeze Spray in a Cryostat In-Reply-To: References: Message-ID: Jennifer, I called Leica as we have 2 cryostats at different locations so we needed to be sure that we could use our current spray that we get from Leica. We were told that their spray with the 1,1,1,2 Tetrafluoroethane is safe to use. I would therefore check to see what you have in stock and if it is this you should follow the guidelines on the can. We do not use spray on possible airborne pathogen cases and we avoid spraying the specimen directly at all times. Hope this helps. Vikki On Tue, May 28, 2019, 1:11 PM Schumacher, Jennifer J via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all, > Looking for some documentation regarding the use of Freeze Spray in > cryostats. > > A Best Practice document would be ideal, but in the absence of such a > document, I would like to hear how others are planning to address the > "recall" by Leica where they do not recommend the use of the Freeze Spray > in their cryostats due to the possibility of a fire (flammable reagent). > > I personally have always had concerns around infection since the specimens > are fresh and using the spray could aerosolize any infectious agents that > are present. > > There is a lot of pressure to freeze things rapidly to meet the FS TAT, so > the battle goes back and forth. > > Any feedback is much appreciated. > > Jennifer > > > ________________________________ > > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this communication in error, please return it to the > sender immediately and delete the original message and any copy of it from > your computer system. If you have any questions concerning this message, > please contact the sender. Disclaimer R001.0 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ben.sylvia at neogenomics.com Wed May 29 07:29:48 2019 From: ben.sylvia at neogenomics.com (Ben Sylvia) Date: Wed, 29 May 2019 12:29:48 +0000 Subject: [Histonet] Neogenomics Careers... Message-ID: <6402976026c44c0c937913f6abb8310f@tp1pvm-mbx2.neogen.local> Hello Histonet, I'm writing to introduce myself and Neogenomics as an employer of choice for Histotechnicians and Histotechnologists. NeoGenomics Laboratories is comprised of a national team of experts in developing and delivering laboratory diagnostic and clinical trial services with a focus in cancer. It is the common purpose of all NeoGenomics employees to save lives by improving patient CARE through Communication, Accuracy, Reliability, and Efficiency. More can be learned about Neogenomics here and I am available any time to discuss career opportunities both available now and to plan for the future. Thank you for supplying this wonderful tool to connect the Histo community. Please let me know how best to format my message for distribution. All the best, Ben Sylvia Talent Engagement Specialist NeoGenomics Laboratories, Inc. 12701 Commonwealth Dr., Suite 9, Fort Myers, FL 33913 USA Phone: 239.768.0600 x 2904 Ben.Sylvia at neogenomics.com www.neogenomics.com/careers This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. E-mail transmission cannot be guaranteed to be secured or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. If verification is required please request a hard-copy version. NeoGenomics Laboratories, Suite 5, 12701 Commonwealth Dr, Fort Myers, FL 33913, http://www.neogenomics.com (2019) From relia1 at earthlink.net Wed May 29 09:01:44 2019 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 29 May 2019 10:01:44 -0400 Subject: [Histonet] ICYMI FREE Resume Tune-up from RELIA Solutions Message-ID: <021401d51627$0ccbb4f0$26631ed0$@earthlink.net> Hi Histopeeps, Resumes they?re not just for job hunting anymore! Did you know it is a great idea to always keep your resume updated? Resumes and CVs are used for many things besides job hunting: Looking at a raise or promotion? ? Does your resume list all of your accomplishments so that you can present them to your supervisor? Ever thought about giving a presentation or writing an article?? ? A great CV always makes a presentation much more polished. How about just for you? ? It feels great to see how far you have come in your career and might help with direction for what?s next. How about if you are transitioning? ? Say going back to permanent work from travel or vice versa or want to move into another area of histology. And of course good old fashioned job hunting! Histopeeps, Let me help you get your resume tuned up!!? It?s free of charge as a service to my Histopeeps!! Here?s what you need to do: Reply to this email with I?m in and we can get started. I look forward to assisting you!! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From kdavoli at gmail.com Wed May 29 10:11:41 2019 From: kdavoli at gmail.com (Kate Davoli) Date: Wed, 29 May 2019 11:11:41 -0400 Subject: [Histonet] Re-embed agar/FFPE tissue in plastic? Message-ID: I have a bunch of precious FFPE samples that were embedded in agar prior to being embedded in wax (so as to orient the tissue easily). The client now wants these samples to be taken backwards out of wax and processed for semithin plastic GMA (JB-4) sectioning. Is that possible? Will the tissue having previously been embedded in wax cause problems for the reagent infiltration or the plastic curing? Will it interfere with the catalyst? I know that taking tissue back through xylenes and alcohols is *supposed *to be able to remove all the wax, but does it really? I need the agar support surrounding the sample to STAY on the sample so that it can stand upright during the plastic curing ... has anybody tried to do double embedding with agar and then JB-4? Does that work? Please help! From Timothy.Morken at ucsf.edu Wed May 29 10:53:08 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 29 May 2019 15:53:08 +0000 Subject: [Histonet] Re-embed agar/FFPE tissue in plastic? In-Reply-To: References: Message-ID: Kate, we regularly re-embed paraffin-embedded tissue in epoxy resin for electron microscopy. We are using very small tissue - kidney, liver, 1mm core biopsies. For that we run thru xylene 4 x 30 min at 60C, 100% ethanol 3 x 10 min, 95, 70, 50% ethanol 5 min each, then to distilled water, then buffer. Then it goes on to processing for resin embedding. The wax is gone and will not interfere with the plastic. The agar will not be affected or affect plastic embedding. We use agar to embed cilia for EM with good results. You might want to do some test tissue to get the timing right before trying on your samples. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Kate Davoli via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, May 29, 2019 8:12 AM To: Histonet Usegroup Subject: [Histonet] Re-embed agar/FFPE tissue in plastic? I have a bunch of precious FFPE samples that were embedded in agar prior to being embedded in wax (so as to orient the tissue easily). The client now wants these samples to be taken backwards out of wax and processed for semithin plastic GMA (JB-4) sectioning. Is that possible? Will the tissue having previously been embedded in wax cause problems for the reagent infiltration or the plastic curing? Will it interfere with the catalyst? I know that taking tissue back through xylenes and alcohols is *supposed *to be able to remove all the wax, but does it really? I need the agar support surrounding the sample to STAY on the sample so that it can stand upright during the plastic curing ... has anybody tried to do double embedding with agar and then JB-4? Does that work? Please help! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=LkO-xDIfH568ZCftTUB3ZPrXyL9zHHABGoLGxN2Wo8U&s=yPsDnCOc5sQ7pkO8EWBEDlVfz3DKYWimotwS2Je7upw&e= From Nancy_Schmitt at pa-ucl.com Thu May 30 10:19:53 2019 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Thu, 30 May 2019 15:19:53 +0000 Subject: [Histonet] BOND question Message-ID: Hello- Checking in to see if anyone else has seen any staining irregularities with a new BOND Max (OR III) or DS9800 detection kits. I appreciate your thoughts, Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From criley at dpspa.com Thu May 30 11:45:03 2019 From: criley at dpspa.com (Charles Riley) Date: Thu, 30 May 2019 12:45:03 -0400 Subject: [Histonet] BOND question In-Reply-To: References: Message-ID: I have not seen any lately. A few quick things to check that may help 1. Do you see any bubbles in the syringe on the side of the machine when it is running? 2. Have you had any liquid level sensing error messages? On Thu, May 30, 2019 at 11:28 AM Nancy Schmitt via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello- > Checking in to see if anyone else has seen any staining irregularities > with a new BOND Max (OR III) or DS9800 detection kits. > I appreciate your thoughts, > > Nancy Schmitt MLT, HT(ASCP) > Pathology Support Services Manager > > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > > NOTICE: This email may contain legally privileged information. The > information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the > sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From Diana.Martinez-Longoria at ecrmc.org Thu May 30 13:13:11 2019 From: Diana.Martinez-Longoria at ecrmc.org (Diana Martinez-Longoria) Date: Thu, 30 May 2019 18:13:11 +0000 Subject: [Histonet] Molecular Biology ASCP In-Reply-To: <46cfa987e7154882a7ed52cf709264a4@ecrmc.org> References: <42512aa9a4ff4d0f8a116660f6eb7585@ecrmc.org>, <46cfa987e7154882a7ed52cf709264a4@ecrmc.org> Message-ID: <41990fcc968b471fb7a63307cabc4276@ecrmc.org> From: Diana Martinez-Longoria Sent: Thursday, May 30, 2019 11:09 AM To: 'histonet at lists.utsouthwestern.edu Subject: Molecular Biology ASCP Good day, I don't know if anyone can help me regarding how does someone become ASCP certified for Molecular Biology in Histopathology? Does anyone know how one can study or take a program that can prepare you to take the ASCP Molecular Biology Exam? Thank you ! Diana Martinez-Longoria Bachelors of Science in Biology Histotechnician (ASCP)cm El Centro Regional Medical Center Phone: 760-339-7267 Fax: 760-339-4570 Email:dmlongoria at ecrmc.org ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. ?? From marktarango at gmail.com Thu May 30 15:10:59 2019 From: marktarango at gmail.com (Mark Tarango) Date: Thu, 30 May 2019 13:10:59 -0700 Subject: [Histonet] Molecular Biology ASCP In-Reply-To: <41990fcc968b471fb7a63307cabc4276@ecrmc.org> References: <42512aa9a4ff4d0f8a116660f6eb7585@ecrmc.org> <46cfa987e7154882a7ed52cf709264a4@ecrmc.org> <41990fcc968b471fb7a63307cabc4276@ecrmc.org> Message-ID: I'm doing a biotech program at a community college that has classes in molecular biology, recombinant DNA technology, Immunology, Flow Cytometry and others. I think these classes will have me well prepared for the MB(ASCP) exam but I also work in the molecular side of the lab in FISH. Mark On Thu, May 30, 2019 at 11:28 AM Diana Martinez-Longoria via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > > From: Diana Martinez-Longoria > Sent: Thursday, May 30, 2019 11:09 AM > To: 'histonet at lists.utsouthwestern.edu > Subject: Molecular Biology ASCP > > > > Good day, > > > I don't know if anyone can help me regarding how does someone become ASCP > certified for Molecular Biology in Histopathology? Does anyone know how one > can study or take a program that can prepare you to take the ASCP Molecular > Biology Exam? Thank you ! > > > Diana Martinez-Longoria > > Bachelors of Science in Biology > > Histotechnician (ASCP)cm > > El Centro Regional Medical Center > > Phone: 760-339-7267 > > Fax: 760-339-4570 > > Email:dmlongoria at ecrmc.org > > > > > ECRMC Confidentiality Notice: This e-mail is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, PLEASE contact the > sender and promptly destroy this e-mail and its attachments. > ? > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Sandra.Etheridge at gov.bc.ca Thu May 30 17:07:32 2019 From: Sandra.Etheridge at gov.bc.ca (Etheridge, Sandra AGRI:EX) Date: Thu, 30 May 2019 22:07:32 +0000 Subject: [Histonet] Tissue Tek TEC 6 Embedding Centre Message-ID: <522ec75ec0c141628dd82e23201ab733@E3PMBX04.idir.BCGOV> Hi everyone, We are looking to purchase a new embedding centre and was wondering if anyone has the new Tissue Tek TEC 6? Our current Tissue Tek TEC is 15 years old and parts are no longer available for repair. We are comparing the TEC 6 to the Leica HistoCore Arcadia system. Just wondering if anyone can give some feedback with regard to pro and cons of either unit. Much appreciated! Sandra Etheridge BC Ministry of Agriculture Plant and Animal Health Centre Histology/IHC 1767 Angus Campbell Road Abbotsford, BC V3G 2M3 # (604) 556-3120 From ewj at pigs.ag Thu May 30 20:13:02 2019 From: ewj at pigs.ag (E. Wayne Johnson) Date: Fri, 31 May 2019 09:13:02 +0800 Subject: [Histonet] Tissue Tek TEC 6 Embedding Centre In-Reply-To: <522ec75ec0c141628dd82e23201ab733@E3PMBX04.idir.BCGOV> References: <522ec75ec0c141628dd82e23201ab733@E3PMBX04.idir.BCGOV> Message-ID: <90afdb5d-90ad-5575-29d4-34c875994c42@pigs.ag> I am skeptical that parts are not available for these relatively new (fifteen years old) machines... Of course reliability is a serious concern in any case. E. Wayne Johnson DVM Enable AgTech Beijing Etheridge, Sandra AGRI:EX via Histonet wrote: > Hi everyone, > > We are looking to purchase a new embedding centre and was wondering if anyone has the new Tissue Tek TEC 6? Our current Tissue Tek TEC is 15 years old and parts are no longer available for repair. > > We are comparing the TEC 6 to the Leica HistoCore Arcadia system. Just wondering if anyone can give some feedback with regard to pro and cons of either unit. > Much appreciated! > > Sandra Etheridge > > BC Ministry of Agriculture > Plant and Animal Health Centre > Histology/IHC > 1767 Angus Campbell Road > Abbotsford, BC V3G 2M3 > # (604) 556-3120 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From wdesalvo.cac at outlook.com Thu May 30 20:44:00 2019 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Fri, 31 May 2019 01:44:00 +0000 Subject: [Histonet] Tissue Tek TEC 6 Embedding Centre In-Reply-To: <90afdb5d-90ad-5575-29d4-34c875994c42@pigs.ag> References: <522ec75ec0c141628dd82e23201ab733@E3PMBX04.idir.BCGOV>, <90afdb5d-90ad-5575-29d4-34c875994c42@pigs.ag> Message-ID: Check with the manufacturer as many in the pathology space are moving to placing 10 use and no longer supporting. As this continues to become more prevalent, the refurbish companies will continue to support. This is similar to the support of some clinical instruments. William DeSalvo ________________________________ From: E. Wayne Johnson via Histonet Sent: Thursday, May 30, 2019 7:13:02 PM To: Etheridge, Sandra AGRI:EX; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Tissue Tek TEC 6 Embedding Centre I am skeptical that parts are not available for these relatively new (fifteen years old) machines... Of course reliability is a serious concern in any case. E. Wayne Johnson DVM Enable AgTech Beijing Etheridge, Sandra AGRI:EX via Histonet wrote: > Hi everyone, > > We are looking to purchase a new embedding centre and was wondering if anyone has the new Tissue Tek TEC 6? Our current Tissue Tek TEC is 15 years old and parts are no longer available for repair. > > We are comparing the TEC 6 to the Leica HistoCore Arcadia system. Just wondering if anyone can give some feedback with regard to pro and cons of either unit. > Much appreciated! > > Sandra Etheridge > > BC Ministry of Agriculture > Plant and Animal Health Centre > Histology/IHC > 1767 Angus Campbell Road > Abbotsford, BC V3G 2M3 > # (604) 556-3120 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek at digestivespecialists.com Fri May 31 10:52:26 2019 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Fri, 31 May 2019 15:52:26 +0000 Subject: [Histonet] CMV Message-ID: Does anyone have a good CMV control block they would be willing to share? Thanks Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com From jmyers1 at aol.com Fri May 31 17:15:01 2019 From: jmyers1 at aol.com (Joe Myers) Date: Fri, 31 May 2019 18:15:01 -0400 Subject: [Histonet] Histonet - Molecular Biology ASCP References: Message-ID: Diana: Unfortunately, there?s no such certification for ?molecular biology in histology? per se; the MB certification offered by the ASCP is generally pursued by individuals who are already certified as an MT or MLT since these types of procedures are usually performed in the ?main lab? (rather than in the histology section). That?s not to say that folks with HT/HTL/CT certification shouldn?t consider pursuing the MB as well if they?d like. As Mark indicated in his response, doing so usually involves taking several courses relating to MB techniques/instrumentation, and although you will occasionally see an MB ?overview? course offered at a histology-society meeting, one would likely need a great deal more classroom/self-paced education and hands-on training to successfully pass the MB exam. Cheers, Joe Myers, M.S., CT(ASCP)QIHC > Message: 1 > Date: Thu, 30 May 2019 18:13:11 +0000 > From: Diana Martinez-Longoria > To: "Histonet at lists.utsouthwestern.edu" > Subject: [Histonet] Molecular Biology ASCP > From: Diana Martinez-Longoria > Sent: Thursday, May 30, 2019 11:09 AM > > Subject: Molecular Biology ASCP > > > > Good day, > > > I don't know if anyone can help me regarding how does someone become ASCP certified for Molecular Biology in Histopathology? Does anyone know how one can study or take a program that can prepare you to take the ASCP Molecular Biology Exam? Thank you ! > > > Diana Martinez-Longoria > > Bachelors of Science in Biology > > Histotechnician (ASCP)cm > > El Centro Regional Medical Center > > Phone: 760-339-7267 > > Fax: 760-339-4570 > > Email:dmlongoria at ecrmc.org > From wdesalvo.cac at outlook.com Fri May 31 18:51:13 2019 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Fri, 31 May 2019 23:51:13 +0000 Subject: [Histonet] Histonet - Molecular Biology ASCP In-Reply-To: References: , Message-ID: If you have the HTL, you are eligible to take the MB. You can also track with education and OJT for one year in molecular lab. William DeSalvo ________________________________ From: Joe Myers via Histonet Sent: Friday, May 31, 2019 4:15:01 PM To: HistoNet Subject: Re: [Histonet] Histonet - Molecular Biology ASCP Diana: Unfortunately, there?s no such certification for ?molecular biology in histology? per se; the MB certification offered by the ASCP is generally pursued by individuals who are already certified as an MT or MLT since these types of procedures are usually performed in the ?main lab? (rather than in the histology section). That?s not to say that folks with HT/HTL/CT certification shouldn?t consider pursuing the MB as well if they?d like. As Mark indicated in his response, doing so usually involves taking several courses relating to MB techniques/instrumentation, and although you will occasionally see an MB ?overview? course offered at a histology-society meeting, one would likely need a great deal more classroom/self-paced education and hands-on training to successfully pass the MB exam. Cheers, Joe Myers, M.S., CT(ASCP)QIHC > Message: 1 > Date: Thu, 30 May 2019 18:13:11 +0000 > From: Diana Martinez-Longoria > To: "Histonet at lists.utsouthwestern.edu" > Subject: [Histonet] Molecular Biology ASCP > From: Diana Martinez-Longoria > Sent: Thursday, May 30, 2019 11:09 AM > > Subject: Molecular Biology ASCP > > > > Good day, > > > I don't know if anyone can help me regarding how does someone become ASCP certified for Molecular Biology in Histopathology? Does anyone know how one can study or take a program that can prepare you to take the ASCP Molecular Biology Exam? Thank you ! > > > Diana Martinez-Longoria > > Bachelors of Science in Biology > > Histotechnician (ASCP)cm > > El Centro Regional Medical Center > > Phone: 760-339-7267 > > Fax: 760-339-4570 > > Email:dmlongoria at ecrmc.org > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet