From kelley.jordan at roche.com Fri Mar 1 12:15:58 2019 From: kelley.jordan at roche.com (Jordan, Kelley) Date: Fri, 1 Mar 2019 13:15:58 -0500 Subject: [Histonet] Histonet Digest, Vol 184, Issue 1 In-Reply-To: References: Message-ID: More bad press in histonet...not sure if we should pass it on to Marketing?? @Will, Who as Teri Blaud at Holy Redeemer Hospital ? Teri is always always bashing us. Kelley Jordan Strategic Account Manager - SC, NC, TN and KY A Member of the Roche Group Ventana Medical Systems Mobile: 803.504.1135 Customer/Technical Support: 1.800.227.2155 kelley.jordan at roche.com www.ventana.com Empowering | Innovation Confidentiality Note: This message is intended only for the use of the named recipient(s) and may contain confidential and/or proprietary information. If you are not the intended recipient, please contact the sender and delete this message. Any unauthorized use of the information contained in this message is prohibited. ---------- Forwarded message --------- From: Date: Fri, Mar 1, 2019 at 1:00 PM Subject: Histonet Digest, Vol 184, Issue 1 To: Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IHC Control Slide Longevity (Sandra Cheasty) 2. Re: Another Dispenser Failure (Terri Braud) 3. Re: IHC Control Slide Longevity (Jan Shivers) 4. Surepath stains (Nancy Schmitt) 5. Re: Another Dispenser Failure (Mark Tarango) 6. Re: [External] Re: Another Dispenser Failure (Bitting, Angela K.) 7. Re: Another Dispenser Failure (Curt) 8. Ventana p16 antibody (Cartun, Richard) ---------- Forwarded message ---------- From: Sandra Cheasty To: "Histonet (histonet at lists.utsouthwestern.edu)" < histonet at lists.utsouthwestern.edu> Cc: Bcc: Date: Thu, 28 Feb 2019 18:56:43 +0000 Subject: [Histonet] IHC Control Slide Longevity Hello all, Do any of you have to cut any of you antibody controls "fresh"? We currently cut the MUM-1 fresh, and the Synaptophysin. (We seldom do a Syanpt., and have found the fresh cut patient tissue has been positive while the not-so-fresh positive control slide is negative. Any comments are - as always, are appreciated. Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ---------- Forwarded message ---------- From: Terri Braud To: "'histonet at lists.utsouthwestern.edu'" Cc: Bcc: Date: Thu, 28 Feb 2019 19:21:53 +0000 Subject: Re: [Histonet] Another Dispenser Failure Another one!!! Our Her2 antibody dispenser failed, LOT #E22628 This one "supposedly" FDA approved. Roche, why do you continue to lie to consumers of your product? You claim you've "fixed" the problem but your Ventana dispensers DON'T WORK! This is patient care! Why don't you care about the customers and patients you are supposed to be serving???? Shame on you. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto: histonet-request at lists.utsouthwestern.edu] Sent: Thursday, February 28, 2019 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 183, Issue 23 Today's Topics: 1. H&E Staining question (Charles Riley) 2. Re: H&E Staining question (Jay Lundgren) 3. FYI- Roche Ventana users (Cassie P. Davis) Message: 3 Date: Thu, 28 Feb 2019 15:23:16 +0000 From: "Cassie P. Davis" To: histonet Subject: [Histonet] FYI- Roche Ventana users Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histoland, I am biting my tongue HARD and just letting you know so it doesn't happend to you. I just got off the phone with Roche here is the heads-up. If one of their anitbody dispensers fails DO NOT put the antibody in one of their prep kits, as soon as you do they consider it off label use. Call customer service immediately and have them overnight a replacement! Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org ---------- Forwarded message ---------- From: Jan Shivers To: Sandra Cheasty Cc: "Histonet (histonet at lists.utsouthwestern.edu)" < histonet at lists.utsouthwestern.edu> Bcc: Date: Thu, 28 Feb 2019 13:29:04 -0600 Subject: Re: [Histonet] IHC Control Slide Longevity Hi Sandy, We cut CD31, CD45, CD45RA, CD117, CD204, and MUM1 controls fresh (same morning as staining; case material as well). We've never had any problems with Synaptophysin; it's always worked very well on unstained controls that are up to a year old (stored in the fridge). Best regards, Jan Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003 at umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Thu, Feb 28, 2019 at 12:57 PM Sandra Cheasty via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello all, > Do any of you have to cut any of you antibody controls > "fresh"? We currently cut the MUM-1 fresh, and the Synaptophysin. (We > seldom do a Syanpt., and have found the fresh cut patient tissue has been > positive while the not-so-fresh positive control slide is negative. > Any comments are - as always, are appreciated. > Cheers, > Sandy > > Sandra J. Cheasty, HT (ASCP) > Histology & Necropsy Supervisor > UW-Madison, School of Veterinary Medicine > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ---------- Forwarded message ---------- From: Nancy Schmitt To: "'histonet at lists.utsouthwestern.edu'" Cc: Bcc: Date: Thu, 28 Feb 2019 20:28:42 +0000 Subject: [Histonet] Surepath stains Hello- This is regarding Surepath pap stain processing on the BD Totalys. We switched to the new Totalys in late November and are having a lot of staining issues. We have had many visits with field service. Is anyone using this and having issues with inconsistent staining? Thank you for your consideration, Nancy United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ---------- Forwarded message ---------- From: Mark Tarango To: Terri Braud Cc: "histonet at lists.utsouthwestern.edu" Bcc: Date: Thu, 28 Feb 2019 12:30:03 -0800 Subject: Re: [Histonet] Another Dispenser Failure I hope everyone is using on-slide controls :-) On Thu, Feb 28, 2019 at 11:52 AM Terri Braud via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Another one!!! > Our Her2 antibody dispenser failed, LOT #E22628 > This one "supposedly" FDA approved. > Roche, why do you continue to lie to consumers of your product? You claim > you've "fixed" the problem but your Ventana dispensers DON'T WORK! > This is patient care! Why don't you care about the customers and patients > you are supposed to be serving???? > Shame on you. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > -----Original Message----- > From: histonet-request at lists.utsouthwestern.edu [mailto: > histonet-request at lists.utsouthwestern.edu] > Sent: Thursday, February 28, 2019 1:00 PM > To: histonet at lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 183, Issue 23 > > > Today's Topics: > 1. H&E Staining question (Charles Riley) > 2. Re: H&E Staining question (Jay Lundgren) > 3. FYI- Roche Ventana users (Cassie P. Davis) > Message: 3 > Date: Thu, 28 Feb 2019 15:23:16 +0000 > From: "Cassie P. Davis" > To: histonet > Subject: [Histonet] FYI- Roche Ventana users > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histoland, > I am biting my tongue HARD and just letting you know so it doesn't > happend to you. I just got off the phone with Roche here is the heads-up. > If one of their anitbody dispensers fails DO NOT put the antibody in one > of their prep kits, as soon as you do they consider it off label use. > Call customer service immediately and have them overnight a replacement! > Cassandra Davis > Histology Technician > AP Laboratory > 302-575-8095 > Email: CDavis at che-east.org > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ---------- Forwarded message ---------- From: "Bitting, Angela K." To: Mark Tarango Cc: Terri Braud , " histonet at lists.utsouthwestern.edu" Bcc: Date: Thu, 28 Feb 2019 21:17:58 +0000 Subject: Re: [Histonet] [External] Re: Another Dispenser Failure Is there any other way! :-O Sent from my iPhone > On Feb 28, 2019, at 3:30 PM, Mark Tarango via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > I hope everyone is using on-slide controls :-) > > On Thu, Feb 28, 2019 at 11:52 AM Terri Braud via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Another one!!! >> Our Her2 antibody dispenser failed, LOT #E22628 >> This one "supposedly" FDA approved. >> Roche, why do you continue to lie to consumers of your product? You claim >> you've "fixed" the problem but your Ventana dispensers DON'T WORK! >> This is patient care! Why don't you care about the customers and patients >> you are supposed to be serving???? >> Shame on you. >> >> Terri L. Braud, HT(ASCP) >> Anatomic Pathology Supervisor >> Laboratory >> Holy Redeemer Hospital >> 1648 Huntingdon Pike >> Meadowbrook, PA 19046 >> ph: 215-938-3689 >> fax: 215-938-3874 >> Care, Comfort, and Heal >> >> -----Original Message----- >> From: histonet-request at lists.utsouthwestern.edu [mailto: >> histonet-request at lists.utsouthwestern.edu] >> Sent: Thursday, February 28, 2019 1:00 PM >> To: histonet at lists.utsouthwestern.edu >> Subject: Histonet Digest, Vol 183, Issue 23 >> >> >> Today's Topics: >> 1. H&E Staining question (Charles Riley) >> 2. Re: H&E Staining question (Jay Lundgren) >> 3. FYI- Roche Ventana users (Cassie P. Davis) >> Message: 3 >> Date: Thu, 28 Feb 2019 15:23:16 +0000 >> From: "Cassie P. Davis" >> To: histonet >> Subject: [Histonet] FYI- Roche Ventana users >> Message-ID: >> Content-Type: text/plain; charset="iso-8859-1" >> >> Hi Histoland, >> I am biting my tongue HARD and just letting you know so it doesn't >> happend to you. I just got off the phone with Roche here is the heads-up. >> If one of their anitbody dispensers fails DO NOT put the antibody in one >> of their prep kits, as soon as you do they consider it off label use. >> Call customer service immediately and have them overnight a replacement! >> Cassandra Davis >> Histology Technician >> AP Laboratory >> 302-575-8095 >> Email: CDavis at che-east.org >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cakbitting%40geisinger.edu%7C54f99c969c034efc787c08d69dbb9222%7C37d46c567c664402a16055c2313b910d%7C0%7C0%7C636869826267838962&sdata=Fa%2BIazZR5ONBVw8DF35Yib5Ev1RTbpySxNuVjo0NobY%3D&reserved=0 >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cakbitting%40geisinger.edu%7C54f99c969c034efc787c08d69dbb9222%7C37d46c567c664402a16055c2313b910d%7C0%7C0%7C636869826267848970&sdata=%2Fdu5DB7SPsE5RRHX9hpCNyGopDRovFnxf9NwQZmjsu4%3D&reserved=0 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ---------- Forwarded message ---------- From: Curt To: Mark Tarango , Terri Braud < tbraud at holyredeemer.com> Cc: "histonet at lists.utsouthwestern.edu" Bcc: Date: Thu, 28 Feb 2019 21:30:20 +0000 Subject: Re: [Histonet] Another Dispenser Failure It's the money they care about... Sent from my Verizon, Samsung Galaxy smartphone -------- Original message -------- From: Mark Tarango via Histonet Date: 2/28/19 12:46 PM (GMT-08:00) To: Terri Braud Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Another Dispenser Failure I hope everyone is using on-slide controls :-) On Thu, Feb 28, 2019 at 11:52 AM Terri Braud via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Another one!!! > Our Her2 antibody dispenser failed, LOT #E22628 > This one "supposedly" FDA approved. > Roche, why do you continue to lie to consumers of your product? You claim > you've "fixed" the problem but your Ventana dispensers DON'T WORK! > This is patient care! Why don't you care about the customers and patients > you are supposed to be serving???? > Shame on you. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > -----Original Message----- > From: histonet-request at lists.utsouthwestern.edu [mailto: > histonet-request at lists.utsouthwestern.edu] > Sent: Thursday, February 28, 2019 1:00 PM > To: histonet at lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 183, Issue 23 > > > Today's Topics: > 1. H&E Staining question (Charles Riley) > 2. Re: H&E Staining question (Jay Lundgren) > 3. FYI- Roche Ventana users (Cassie P. Davis) > Message: 3 > Date: Thu, 28 Feb 2019 15:23:16 +0000 > From: "Cassie P. Davis" > To: histonet > Subject: [Histonet] FYI- Roche Ventana users > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histoland, > I am biting my tongue HARD and just letting you know so it doesn't > happend to you. I just got off the phone with Roche here is the heads-up. > If one of their anitbody dispensers fails DO NOT put the antibody in one > of their prep kits, as soon as you do they consider it off label use. > Call customer service immediately and have them overnight a replacement! > Cassandra Davis > Histology Technician > AP Laboratory > 302-575-8095 > Email: CDavis at che-east.org > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. ---------- Forwarded message ---------- From: "Cartun, Richard" To: "histonet at lists.utsouthwestern.edu" Cc: Bcc: Date: Thu, 28 Feb 2019 22:47:46 +0000 Subject: [Histonet] Ventana p16 antibody We use the Roche/Ventana p16 antibody (clone E6H4(tm)) on the Leica Bond Max. We are having a difficult time trying to order the antibody. Can someone verify the catalog number (705-4713) for me? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDavis at che-east.org Fri Mar 1 12:16:24 2019 From: CDavis at che-east.org (Cassie P. Davis) Date: Fri, 1 Mar 2019 18:16:24 +0000 Subject: [Histonet] Ventana p16 antibody Message-ID: <11170a56bf4c4850860d5958e2590deb@che-east.org> Dr. Cartun, your catalog # is one digit off, it may be from when they got FDA approval and changed the numbers. The catalog number for the "new" FDA approved p16 now labeled p16 HISTO BIOMKR (from mt printout) is 705-4793 Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From albert.dotson at duke.edu Sat Mar 2 12:56:58 2019 From: albert.dotson at duke.edu (Bert Dotson) Date: Sat, 2 Mar 2019 18:56:58 +0000 Subject: [Histonet] Opportunities at Duke in Durham, NC Message-ID: Duke University Health System Clinical Laboratories has opportunities for the following: ? Lead Pathology Assistant/Lab supervisor - must be graduate of a NAACLS accredited PA training program. ? Pathologists' Assistant - must be graduate of a NAACLS accredited PA training program. ? Lead Histology Technologist for accessioning/frozen section lab - certified or registry eligible, must have bachelor's degree. ? Second and third shift Histology Technicians in a high-volume, growing and busy environment - certified or registry eligible. Please apply through our website: https://hr.duke.edu/careers/apply PA/ Lead PA search for requisition: 401515507 Lead Histology Technologist search for requisition: 401558877 Night shift Histology Technician search for requisition: 401558849 Second shift Histology Technician search for requisition: 401521890 Duke University Medical Center 2301 Erwin Road, Durham, NC 27710 1019 Beds ? 30 pathologists ? 50,000 surgical cases annually Duke is a world-class academic and health institution ranked among the top 20 best hospitals and ranking high performance in nine adult specialties. Duke is also home of the first established Pathologists' Assistant program. Duke University Medical Center is located in in central North Carolina with both the beautiful Appalachian mountains and the coastal beaches just a couple of hours away. Duke University is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status. Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas-an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values. Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department. Bert Dotson, MBA, HTL (ASCP) Manager, Duke Surgical Pathology Laboratories HAFS Building T Level Rm 0664 DUMC 3618 Durham, NC 27705 The information in this electronic mail is sensitive, protected information intended only for the addressee(s). Any other person, including anyone who believes he/she might have received it due to an addressing error, is requested to notify the sender immediately by return electronic mail, and to delete it without further reading or retention. The information is not to be forwarded or shared in any manner unless in compliance with the Duke University policies on confidentiality and/or with the approval of the sender. From mpence at grhs.net Mon Mar 4 14:51:32 2019 From: mpence at grhs.net (Mike Pence) Date: Mon, 4 Mar 2019 20:51:32 +0000 Subject: [Histonet] Pathology QA Reports Message-ID: How long are we required to keep routine QA reports? Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 mpence at grhs.net | www.greatrivermedical.org. www.Facebook.com/GreatRiverHealthSystems | www.Twitter/GreatRiverMed Information in this communication, including attachments, is confidential and intended only for the addressee(s). This communication may contain privileged, confidential, proprietary or trade secret information entitled to protection or exemption from disclosure under law. If you are not an intended recipient, please know that any use, distribution or copying of this communication, or any action taken based on the information in this communication, is unauthorized and may be unlawful. If you received this communication in error, please notify the sender and delete this communication from your device. From tbraud at holyredeemer.com Tue Mar 5 09:23:26 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 5 Mar 2019 15:23:26 +0000 Subject: [Histonet] Roche "bashing" Message-ID: <48E053DDF6CE074DB6A7414BA05403F8013BEE31B5@HRHEX02-HOS.holyredeemer.local> Such a wonderful insight into the thought process of Roche. Accused of bashing when I am telling the truth about failed lots when they claim the problem is fixed. If only they would put that much effort into repairing the problem and fostering better customer relations, than how to market their product around this annoying truth. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Re: Histonet Digest, Vol 184, Issue 1 (Jordan, Kelley) Message: 1 Date: Fri, 1 Mar 2019 13:15:58 -0500 From: "Jordan, Kelley" Content-Type: text/plain; charset="UTF-8" More bad press in histonet...not sure if we should pass it on to Marketing?? @Will, Who as Teri Blaud at Holy Redeemer Hospital ? Teri is always always bashing us. Kelley Jordan Strategic Account Manager - SC, NC, TN and KY A Member of the Roche Group Ventana Medical Systems Mobile: 803.504.1135 Customer/Technical Support: 1.800.227.2155 kelley.jordan at roche.com www.ventana.com From relia1 at earthlink.net Tue Mar 5 09:50:29 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 5 Mar 2019 10:50:29 -0500 Subject: [Histonet] Histotechnology Professionals Day is Sunday March 10th Happy Histo Day!! Message-ID: <000001d4d36b$290a2880$7b1e7980$@earthlink.net> Hi Histonetters, Happy Histotechnology Professionals Day! I want to thank you not only for the job that you do and the lives that you save but also for allowing me to work with you in your careers. In all my years of recruiting I have never worked with a more professional, appreciative, honest and truly enjoyable group of people. Kudos to you!! I am curious as to how you are planning on celebrating. After I finish sending this email out to you I will be contacting each and every one of my 1000+ client/employers to let them know that March 10 is National Histotechnology Professionals Day. Hopefully, if they weren't already doing something to show their appreciation they are planning to now!! Histonetters, since HPD falls on a Sunday this year, maybe it is a good time to reflect on your career, where you are, what you want, where you would like to be and how are you going to get there? If you aren?t considering a job change: ? Is it time to join/renew your NSH membership? ? Check out your state society? ? Join the Histonet? ? Take some CEUs to stay up to date and learn some new skills? ? Get that state license for the place you want to move to one day? ? Start studying for that QIHC? If you are or might be considering a job change now or in the near future: ? Does your resume need to be updated? ? Have you considered what you are looking for and where? ? Have you gotten the state license if it is required before you go? ? Have we spoken recently? Please feel free to contact me by phone toll free at 866-607-3542 or email: relia1 at earthlink.net if you want information or help with any of the items listed. My services are always free of charge to you. If you have any friends that might benefit from receiving this please feel free to pass it along. I Currently Have Amazing Leadership Opportunities In: ? Georgia ? Ohio ? Wisconsin ? Massachusetts I have exciting opportunities for Histology Techs in: ? Alabama ? Florida ? New York ? Michigan ? Massachusetts ? California ? Maryland If you would like more info on any of these opportunities or help with a job search in another area please shoot me an email at relia1 at earthlink.net to set up a time to chat at YOUR convenience!! Don?t forget to shoot me back an email to share what you did for Histotechnology Professionals Day. I really want to know ? Happy Histo Day!! >Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.twitter.com/pamatrelia From RobertoJimenez at texashealth.org Tue Mar 5 10:15:10 2019 From: RobertoJimenez at texashealth.org (Jimenez, Roberto) Date: Tue, 5 Mar 2019 16:15:10 +0000 Subject: [Histonet] HPV ISH Message-ID: Good morning, Does anyone have HPV-ISH working utilizing Ventana probes?? Many Thanks, Roberto Jimenez, HT (ASCP) (817)250-5645 The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From marktarango at gmail.com Tue Mar 5 10:39:09 2019 From: marktarango at gmail.com (Mark Tarango) Date: Tue, 5 Mar 2019 08:39:09 -0800 Subject: [Histonet] Histonet Digest, Vol 184, Issue 1 In-Reply-To: References: Message-ID: How about passing on to the department that could fix the issue? Where's the empowering innovation? On Fri, Mar 1, 2019 at 10:35 AM Jordan, Kelley via Histonet < histonet at lists.utsouthwestern.edu> wrote: > More bad press in histonet...not sure if we should pass it on to > Marketing?? > > @Will, Who as Teri Blaud at Holy Redeemer Hospital ? Teri is always > always bashing us. > Kelley Jordan > > Strategic Account Manager - SC, NC, TN and KY > > > A Member of the Roche Group > > Ventana Medical Systems > > Mobile: 803.504.1135 > Customer/Technical Support: 1.800.227.2155 > > kelley.jordan at roche.com > > www.ventana.com > > > > Empowering | Innovation > > > Confidentiality Note: This message is intended only for the use of the > named recipient(s) and may contain confidential and/or proprietary > information. If you are not the intended recipient, please contact the > sender and delete this message. Any unauthorized use of the information > contained in this message is prohibited. > > histonet > From saby_joseph_a at yahoo.com Tue Mar 5 10:47:52 2019 From: saby_joseph_a at yahoo.com (Joseph Saby) Date: Tue, 5 Mar 2019 16:47:52 +0000 (UTC) Subject: [Histonet] Histonet Digest, Vol 184, Issue 1 In-Reply-To: References: Message-ID: <943750970.9433210.1551804472121@mail.yahoo.com> The more important question is who the h*ll is Kelly Jordan. Terri has established her competency and reputation in NSH and the Histonet for probably over 20 years. You have done your company a great disservice. People will remember you, probably not as you would wish. Joe Saby, retired On Tuesday, March 5, 2019, 11:42:30 AM EST, Mark Tarango via Histonet wrote: How about passing on to the department that could fix the issue?? Where's the empowering innovation? On Fri, Mar 1, 2019 at 10:35 AM Jordan, Kelley via Histonet < histonet at lists.utsouthwestern.edu> wrote: > More bad press in histonet...not sure if we should pass it on to > Marketing?? > > @Will, Who as Teri Blaud at? Holy Redeemer Hospital ? Teri is always > always bashing us. > Kelley Jordan > > Strategic Account Manager - SC, NC, TN and KY > > > A Member of the Roche Group > > Ventana Medical Systems > > Mobile:? 803.504.1135 > Customer/Technical Support: 1.800.227.2155 > > kelley.jordan at roche.com > > www.ventana.com > > > > Empowering | Innovation > > > Confidentiality Note: This message is intended only for the use of the > named recipient(s) and may contain confidential and/or proprietary > information. If you are not the intended recipient, please contact the > sender and delete this message. Any unauthorized use of the information > contained in this message is prohibited. > > histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford at DignityHealth.org Tue Mar 5 12:30:23 2019 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Tue, 5 Mar 2019 18:30:23 +0000 Subject: [Histonet] Frozen section room Message-ID: Good Morning, I have a question about how much reagents you can keep in a frozen section room. Our frozen section is a little room off of Surgery. When it was designed now one thought to ask us what it was going to need so there is little ventilation down there besides the typical room output and input. No hood!! We have a small H&E stain line, DQ and Toluidine Blue. Can I keep a small amount of each reagent down there for topping off and changing out reagents? I was thinking Pint bottles. I am pretty sure there is regulations on this! Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you From tbraud at holyredeemer.com Tue Mar 5 13:57:37 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 5 Mar 2019 19:57:37 +0000 Subject: [Histonet] Ventana dispensers - last word Message-ID: <48E053DDF6CE074DB6A7414BA05403F8013BEE344D@HRHEX02-HOS.holyredeemer.local> To those of you who wrote in support of my comments, thank you sincerely. To the Company's credit, I did receive a call of apology and a listening ear concerning the ongoing problems associated with the dispensers. I sincerely hope they understand the frustration that such a problem can cause. I want to state that I really do like the Benchmark Ultra, but the instrument is only as good as the individual parts. Based on many private responses that I received, I think that dispenser failure is probably being vastly underreported. I can only hope that a moment's indiscretion on their part will lead to a more open dialogue in their company concerning a true solution to this problem. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From bakevictoria at gmail.com Tue Mar 5 14:39:21 2019 From: bakevictoria at gmail.com (Victoria Baker) Date: Tue, 5 Mar 2019 15:39:21 -0500 Subject: [Histonet] Ventana dispensers - last word In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8013BEE344D@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8013BEE344D@HRHEX02-HOS.holyredeemer.local> Message-ID: Terri, We went through hell with the Roche dispensers in late 2017 to early 2018. The only thing positive I have to say is that the field engineer and our FAS are great. I have little respect for the sales staff or inside support. We pay top dollar for these instruments and the required reagents and all Roche shows they are truly concerned with is the next sale We also had a sit-down with their scientific people as well, and here we are again. We haven't had the magnitude of failures this time but it is disheartening to see that what they said they were going to fix has not been fixed. I was also wondering why they would send product failure notifications to clinicians and not to the laboratories that are using the reagents. I got a notification from one of our oncology groups - but I never received one in our lab. Ventana doesn't exist and I miss that very much. With each buy out, merger or supposed partnership that I see with suppliers and manufacturers things are turned more to the bottom line and in the end the patient suffers and it's wrong. But that's big business. Vikki On Mar 5, 2019 3:05 PM, "Terri Braud via Histonet" < histonet at lists.utsouthwestern.edu> wrote: To those of you who wrote in support of my comments, thank you sincerely. To the Company's credit, I did receive a call of apology and a listening ear concerning the ongoing problems associated with the dispensers. I sincerely hope they understand the frustration that such a problem can cause. I want to state that I really do like the Benchmark Ultra, but the instrument is only as good as the individual parts. Based on many private responses that I received, I think that dispenser failure is probably being vastly underreported. I can only hope that a moment's indiscretion on their part will lead to a more open dialogue in their company concerning a true solution to this problem. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegghm at hotmail.com Tue Mar 5 17:10:30 2019 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Tue, 5 Mar 2019 23:10:30 +0000 Subject: [Histonet] Histonet Digest, Vol 184, Issue 1 In-Reply-To: References: , Message-ID: Maybe that is the trouble, they keep passing these technical issues on to Marketing instead of R&D. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com From pruegghm at hotmail.com Tue Mar 5 17:11:34 2019 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Tue, 5 Mar 2019 23:11:34 +0000 Subject: [Histonet] Histonet Digest, Vol 184, Issue 1 In-Reply-To: <943750970.9433210.1551804472121@mail.yahoo.com> References: , <943750970.9433210.1551804472121@mail.yahoo.com> Message-ID: here here Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Joseph Saby Sent: Tuesday, March 5, 2019 9:47 AM To: Jordan, Kelley; Mark Tarango Cc: HistoNet Subject: Re: [Histonet] Histonet Digest, Vol 184, Issue 1 The more important question is who the h*ll is Kelly Jordan. Terri has established her competency and reputation in NSH and the Histonet for probably over 20 years. You have done your company a great disservice. People will remember you, probably not as you would wish. Joe Saby, retired On Tuesday, March 5, 2019, 11:42:30 AM EST, Mark Tarango via Histonet wrote: How about passing on to the department that could fix the issue? Where's the empowering innovation? On Fri, Mar 1, 2019 at 10:35 AM Jordan, Kelley via Histonet < histonet at lists.utsouthwestern.edu> wrote: > More bad press in histonet...not sure if we should pass it on to > Marketing?? > > @Will, Who as Teri Blaud at Holy Redeemer Hospital ? Teri is always > always bashing us. > Kelley Jordan > > Strategic Account Manager - SC, NC, TN and KY > > > A Member of the Roche Group > > Ventana Medical Systems > > Mobile: 803.504.1135 > Customer/Technical Support: 1.800.227.2155 > > kelley.jordan at roche.com > > www.ventana.com > > > > Empowering | Innovation > > > Confidentiality Note: This message is intended only for the use of the > named recipient(s) and may contain confidential and/or proprietary > information. If you are not the intended recipient, please contact the > sender and delete this message. Any unauthorized use of the information > contained in this message is prohibited. > > histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mprice63 at live.com Tue Mar 5 18:06:09 2019 From: mprice63 at live.com (Marsha Price) Date: Wed, 6 Mar 2019 00:06:09 +0000 Subject: [Histonet] Histotech needed immediately In-Reply-To: References: Message-ID: A dermpath lab in Glenwood, Colorado is in immediate need for a high complexity testing qualified histology tech. If you are able to fill in for them on an emergency basis, please respond Thank you, Marsha Price Get Outlook for iOS ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, March 5, 2019 12:19 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 184, Issue 4 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://eur02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7C%7C8788e5c53ef7495654f508d6a197093b%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636874067400078267&sdata=lCrPBT2dLMkk5Xn4Vm3jHcz%2B%2FG1I%2F2xa6IQnfow6Tdw%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Pathology QA Reports (Mike Pence) 2. Re: Roche "bashing" (Terri Braud) 3. Histotechnology Professionals Day is Sunday March 10th Happy Histo Day!! (Pam Barker) 4. HPV ISH (Jimenez, Roberto) 5. Re: Histonet Digest, Vol 184, Issue 1 (Mark Tarango) 6. Re: Histonet Digest, Vol 184, Issue 1 (Joseph Saby) ---------------------------------------------------------------------- Message: 1 Date: Mon, 4 Mar 2019 20:51:32 +0000 From: Mike Pence To: "histonet at lists.utsouthwestern.edu" , "histonet-bounces at lists.utsouthwestern.edu" Subject: [Histonet] Pathology QA Reports Message-ID: Content-Type: text/plain; charset="us-ascii" How long are we required to keep routine QA reports? Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 mpence at grhs.net | https://eur02.safelinks.protection.outlook.com/?url=www.greatrivermedical.org&data=02%7C01%7C%7C8788e5c53ef7495654f508d6a197093b%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636874067400088278&sdata=mHZcD%2BnvjzMUPrFp%2B0uBxf32YRV4UCUYfS0IS1RRXYk%3D&reserved=0. https://eur02.safelinks.protection.outlook.com/?url=www.Facebook.com%2FGreatRiverHealthSystems&data=02%7C01%7C%7C8788e5c53ef7495654f508d6a197093b%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636874067400088278&sdata=SIX3scjPxAs1wy2D0wzKZfhEKadpmwqz1j0xpxbjJvI%3D&reserved=0 | https://eur02.safelinks.protection.outlook.com/?url=www.Twitter%2FGreatRiverMed&data=02%7C01%7C%7C8788e5c53ef7495654f508d6a197093b%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636874067400088278&sdata=Yjt2cDHC3pzbPqv%2F2L%2BwYUQeeGwtz9LHdq8H09nCBSI%3D&reserved=0 Information in this communication, including attachments, is confidential and intended only for the addressee(s). This communication may contain privileged, confidential, proprietary or trade secret information entitled to protection or exemption from disclosure under law. If you are not an intended recipient, please know that any use, distribution or copying of this communication, or any action taken based on the information in this communication, is unauthorized and may be unlawful. If you received this communication in error, please notify the sender and delete this communication from your device. ------------------------------ Message: 2 Date: Tue, 5 Mar 2019 15:23:26 +0000 From: "Terri Braud" To: "'histonet at lists.utsouthwestern.edu'" Cc: "'kelley.jordan at roche.com'" Subject: Re: [Histonet] Roche "bashing" Message-ID: <48E053DDF6CE074DB6A7414BA05403F8013BEE31B5 at HRHEX02-HOS.holyredeemer.local> Content-Type: text/plain; charset="us-ascii" Such a wonderful insight into the thought process of Roche. Accused of bashing when I am telling the truth about failed lots when they claim the problem is fixed. If only they would put that much effort into repairing the problem and fostering better customer relations, than how to market their product around this annoying truth. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Re: Histonet Digest, Vol 184, Issue 1 (Jordan, Kelley) Message: 1 Date: Fri, 1 Mar 2019 13:15:58 -0500 From: "Jordan, Kelley" Content-Type: text/plain; charset="UTF-8" More bad press in histonet...not sure if we should pass it on to Marketing?? @Will, Who as Teri Blaud at Holy Redeemer Hospital ? Teri is always always bashing us. Kelley Jordan Strategic Account Manager - SC, NC, TN and KY A Member of the Roche Group Ventana Medical Systems Mobile: 803.504.1135 Customer/Technical Support: 1.800.227.2155 kelley.jordan at roche.com https://eur02.safelinks.protection.outlook.com/?url=www.ventana.com&data=02%7C01%7C%7C8788e5c53ef7495654f508d6a197093b%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636874067400098289&sdata=AUyZFzZVVp%2FnAIJxHCr85ZbMM6XFsVCXGbGgcMji4XU%3D&reserved=0 ------------------------------ Message: 3 Date: Tue, 5 Mar 2019 10:50:29 -0500 From: "Pam Barker" To: "Histonetters Histonet" Subject: [Histonet] Histotechnology Professionals Day is Sunday March 10th Happy Histo Day!! Message-ID: <000001d4d36b$290a2880$7b1e7980$@earthlink.net> Content-Type: text/plain; charset="utf-8" Hi Histonetters, Happy Histotechnology Professionals Day! I want to thank you not only for the job that you do and the lives that you save but also for allowing me to work with you in your careers. In all my years of recruiting I have never worked with a more professional, appreciative, honest and truly enjoyable group of people. Kudos to you!! I am curious as to how you are planning on celebrating. After I finish sending this email out to you I will be contacting each and every one of my 1000+ client/employers to let them know that March 10 is National Histotechnology Professionals Day. Hopefully, if they weren't already doing something to show their appreciation they are planning to now!! Histonetters, since HPD falls on a Sunday this year, maybe it is a good time to reflect on your career, where you are, what you want, where you would like to be and how are you going to get there? If you aren?t considering a job change: ? Is it time to join/renew your NSH membership? ? Check out your state society? ? Join the Histonet? ? Take some CEUs to stay up to date and learn some new skills? ? Get that state license for the place you want to move to one day? ? Start studying for that QIHC? If you are or might be considering a job change now or in the near future: ? Does your resume need to be updated? ? Have you considered what you are looking for and where? ? Have you gotten the state license if it is required before you go? ? Have we spoken recently? Please feel free to contact me by phone toll free at 866-607-3542 or email: relia1 at earthlink.net if you want information or help with any of the items listed. My services are always free of charge to you. If you have any friends that might benefit from receiving this please feel free to pass it along. I Currently Have Amazing Leadership Opportunities In: ? Georgia ? Ohio ? Wisconsin ? Massachusetts I have exciting opportunities for Histology Techs in: ? Alabama ? Florida ? New York ? Michigan ? Massachusetts ? California ? Maryland If you would like more info on any of these opportunities or help with a job search in another area please shoot me an email at relia1 at earthlink.net to set up a time to chat at YOUR convenience!! Don?t forget to shoot me back an email to share what you did for Histotechnology Professionals Day. I really want to know ? Happy Histo Day!! >Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://eur02.safelinks.protection.outlook.com/?url=www.facebook.comPamBarkerRELIA&data=02%7C01%7C%7C8788e5c53ef7495654f508d6a197093b%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636874067400098289&sdata=1VodXE1djGUokpUfjS8dwDHrK3RRNbZ7w3jeQcVs1cE%3D&reserved=0 https://eur02.safelinks.protection.outlook.com/?url=www.linkedin.com%2Freliasolutions&data=02%7C01%7C%7C8788e5c53ef7495654f508d6a197093b%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636874067400098289&sdata=ExJ45kOsMGouYcnodhQ2OsLRLY4Uy1fgJl5fmCOhJRo%3D&reserved=0 https://eur02.safelinks.protection.outlook.com/?url=www.twitter.com%2Fpamatrelia&data=02%7C01%7C%7C8788e5c53ef7495654f508d6a197093b%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636874067400098289&sdata=ErAVZC1L1z06jhzyZZRyxmDNmDg2imugJ2z8N51DR%2F8%3D&reserved=0 ------------------------------ Message: 4 Date: Tue, 5 Mar 2019 16:15:10 +0000 From: "Jimenez, Roberto" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] HPV ISH Message-ID: Content-Type: text/plain; charset="us-ascii" Good morning, Does anyone have HPV-ISH working utilizing Ventana probes?? Many Thanks, Roberto Jimenez, HT (ASCP) (817)250-5645 The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. ------------------------------ Message: 5 Date: Tue, 5 Mar 2019 08:39:09 -0800 From: Mark Tarango To: "Jordan, Kelley" Cc: HistoNet Subject: Re: [Histonet] Histonet Digest, Vol 184, Issue 1 Message-ID: Content-Type: text/plain; charset="UTF-8" How about passing on to the department that could fix the issue? Where's the empowering innovation? On Fri, Mar 1, 2019 at 10:35 AM Jordan, Kelley via Histonet < histonet at lists.utsouthwestern.edu> wrote: > More bad press in histonet...not sure if we should pass it on to > Marketing?? > > @Will, Who as Teri Blaud at Holy Redeemer Hospital ? Teri is always > always bashing us. > Kelley Jordan > > Strategic Account Manager - SC, NC, TN and KY > > > A Member of the Roche Group > > Ventana Medical Systems > > Mobile: 803.504.1135 > Customer/Technical Support: 1.800.227.2155 > > kelley.jordan at roche.com > > https://eur02.safelinks.protection.outlook.com/?url=www.ventana.com&data=02%7C01%7C%7C8788e5c53ef7495654f508d6a197093b%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636874067400098289&sdata=AUyZFzZVVp%2FnAIJxHCr85ZbMM6XFsVCXGbGgcMji4XU%3D&reserved=0 > > > > Empowering | Innovation > > > Confidentiality Note: This message is intended only for the use of the > named recipient(s) and may contain confidential and/or proprietary > information. If you are not the intended recipient, please contact the > sender and delete this message. Any unauthorized use of the information > contained in this message is prohibited. > > histonet > ------------------------------ Message: 6 Date: Tue, 5 Mar 2019 16:47:52 +0000 (UTC) From: Joseph Saby To: "Jordan, Kelley" , Mark Tarango Cc: HistoNet Subject: Re: [Histonet] Histonet Digest, Vol 184, Issue 1 Message-ID: <943750970.9433210.1551804472121 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 The more important question is who the h*ll is Kelly Jordan. Terri has established her competency and reputation in NSH and the Histonet for probably over 20 years. You have done your company a great disservice. People will remember you, probably not as you would wish. Joe Saby, retired On Tuesday, March 5, 2019, 11:42:30 AM EST, Mark Tarango via Histonet wrote: How about passing on to the department that could fix the issue?? Where's the empowering innovation? On Fri, Mar 1, 2019 at 10:35 AM Jordan, Kelley via Histonet < histonet at lists.utsouthwestern.edu> wrote: > More bad press in histonet...not sure if we should pass it on to > Marketing?? > > @Will, Who as Teri Blaud at? Holy Redeemer Hospital ? Teri is always > always bashing us. > Kelley Jordan > > Strategic Account Manager - SC, NC, TN and KY > > > A Member of the Roche Group > > Ventana Medical Systems > > Mobile:? 803.504.1135 > Customer/Technical Support: 1.800.227.2155 > > kelley.jordan at roche.com > > https://eur02.safelinks.protection.outlook.com/?url=www.ventana.com&data=02%7C01%7C%7C8788e5c53ef7495654f508d6a197093b%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636874067400098289&sdata=AUyZFzZVVp%2FnAIJxHCr85ZbMM6XFsVCXGbGgcMji4XU%3D&reserved=0 > > > > Empowering | Innovation > > > Confidentiality Note: This message is intended only for the use of the > named recipient(s) and may contain confidential and/or proprietary > information. If you are not the intended recipient, please contact the > sender and delete this message. Any unauthorized use of the information > contained in this message is prohibited. > > histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://eur02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7C%7C8788e5c53ef7495654f508d6a197093b%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636874067400108282&sdata=oUQ2ZGXEdFUgxsyCiXCpgDfSjfeA4s%2F8HecPKm5XDM8%3D&reserved=0 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://eur02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7C%7C8788e5c53ef7495654f508d6a197093b%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636874067400108282&sdata=oUQ2ZGXEdFUgxsyCiXCpgDfSjfeA4s%2F8HecPKm5XDM8%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 184, Issue 4 **************************************** From crussell2671 at icloud.com Tue Mar 5 18:30:05 2019 From: crussell2671 at icloud.com (Cynthia Russell) Date: Tue, 5 Mar 2019 19:30:05 -0500 Subject: [Histonet] Immediate histotech in Colorado Message-ID: Will they pay travel and expenses? Sent from my iPad From ronald.kusters at pfmmedical.com Wed Mar 6 06:50:26 2019 From: ronald.kusters at pfmmedical.com (Kusters, Ronald) Date: Wed, 6 Mar 2019 12:50:26 +0000 Subject: [Histonet] Roche "bashing" In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8013BEE31B5@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8013BEE31B5@HRHEX02-HOS.holyredeemer.local> Message-ID: Hi Terri, This phrase isn't mine but nevertheless I'm gonna use it: Customer support/service is not a department but 100% a mentality. I'm amazed by what you describe and to me there is a long way to go to some company to understand that we deal with cancer diagnostics, questions of life and dead. Money shouldn't be on the radar for that matters..... Success in getting your issue solved to YOUR satisfaction! Ronald Mit freundlichen Gr??en/Kind Regards Ronald Kusters Global Sales Manager Feather Brand Histotechnology pfm medical ag Wankelstra?e 60 50996 K?ln, Germany T +49 (2236) 9641 660 F +49 (2236) 9641 99 660 M +49 (171) 9144343 -----Original Message----- From: Terri Braud [mailto:tbraud at holyredeemer.com] Sent: 05 March 2019 16:23 To: 'histonet at lists.utsouthwestern.edu' Cc: 'kelley.jordan at roche.com' Subject: Re: [Histonet] Roche "bashing" Such a wonderful insight into the thought process of Roche. Accused of bashing when I am telling the truth about failed lots when they claim the problem is fixed. If only they would put that much effort into repairing the problem and fostering better customer relations, than how to market their product around this annoying truth. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Re: Histonet Digest, Vol 184, Issue 1 (Jordan, Kelley) Message: 1 Date: Fri, 1 Mar 2019 13:15:58 -0500 From: "Jordan, Kelley" Content-Type: text/plain; charset="UTF-8" More bad press in histonet...not sure if we should pass it on to Marketing?? @Will, Who as Teri Blaud at Holy Redeemer Hospital ? Teri is always always bashing us. Kelley Jordan Strategic Account Manager - SC, NC, TN and KY A Member of the Roche Group Ventana Medical Systems Mobile: 803.504.1135 Customer/Technical Support: 1.800.227.2155 kelley.jordan at roche.com www.ventana.com From bakevictoria at gmail.com Wed Mar 6 07:03:45 2019 From: bakevictoria at gmail.com (Victoria Baker) Date: Wed, 6 Mar 2019 08:03:45 -0500 Subject: [Histonet] Roche "bashing" In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8013BEE31B5@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8013BEE31B5@HRHEX02-HOS.holyredeemer.local> Message-ID: Kelly, It is not bashing, it is frustration and concern. We need our instruments and reagents to work so that we deliver reliable and consistent results to our pathologists. They trust our work and dedication to them and ultimately the patient. When something like this happens and we can't get a real resolution it undermines many things, but trust is first for me. Vikki On Tue, Mar 5, 2019, 10:44 AM Terri Braud via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Such a wonderful insight into the thought process of Roche. Accused of > bashing when I am telling the truth about failed lots when they claim the > problem is fixed. > If only they would put that much effort into repairing the problem and > fostering better customer relations, than how to market their product > around this annoying truth. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Today's Topics: > > 1. Re: Histonet Digest, Vol 184, Issue 1 (Jordan, Kelley) > Message: 1 > Date: Fri, 1 Mar 2019 13:15:58 -0500 > From: "Jordan, Kelley" > Content-Type: text/plain; charset="UTF-8" > More bad press in histonet...not sure if we should pass it on to > Marketing?? > @Will, Who as Teri Blaud at Holy Redeemer Hospital ? Teri is always > always bashing us. > Kelley Jordan > Strategic Account Manager - SC, NC, TN and KY > A Member of the Roche Group > Ventana Medical Systems > Mobile: 803.504.1135 > Customer/Technical Support: 1.800.227.2155 > kelley.jordan at roche.com > www.ventana.com > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jsjurczak at comcast.net Wed Mar 6 09:57:57 2019 From: jsjurczak at comcast.net (jsjurczak at comcast.net) Date: Wed, 06 Mar 2019 18:57:57 +0300 Subject: [Histonet] =?utf-8?q?Roche_=22bashing=22?= In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F8013BEE31B5@HRHEX02-HOS.holyredeemer.local> Message-ID: <1551887877.796709079@f29.my.com> Like all employees at big pharma companies, Kelly has been indoctrinated into believing that her company, her products, and the service rendered is perfect. To believe otherwise is a quick way to find the door out. As a corollary, there can be no problems other than user error ones.? Sent from myMail for iOS Wednesday, March 6, 2019, 7:03 AM -0600 from histonet at lists.utsouthwestern.edu : >Kelly, > >It is not bashing, it is frustration and concern. > >We need our instruments and reagents to work so that we deliver reliable >and consistent results to our pathologists. They trust our work and >dedication to them and ultimately the patient. When something like this >happens and we can't get a real resolution it undermines many things, but >trust is first for me. > >Vikki > >On Tue, Mar 5, 2019, 10:44 AM Terri Braud via Histonet < >histonet at lists.utsouthwestern.edu > wrote: > >> Such a wonderful insight into the thought process of Roche. Accused of >> bashing when I am telling the truth about failed lots when they claim the >> problem is fixed. >> If only they would put that much effort into repairing the problem and >> fostering better customer relations, than how to market their product >> around this annoying truth. >> >> Terri L. Braud, HT(ASCP) >> Anatomic Pathology Supervisor >> Laboratory >> Holy Redeemer Hospital >> 1648 Huntingdon Pike >> Meadowbrook, PA 19046 >> ph: 215-938-3689 >> fax: 215-938-3874 >> Care, Comfort, and Heal >> >> Today's Topics: >> >> 1. Re: Histonet Digest, Vol 184, Issue 1 (Jordan, Kelley) >> Message: 1 >> Date: Fri, 1 Mar 2019 13:15:58 -0500 >> From: "Jordan, Kelley" < kelley.jordan at roche.com > >> Content-Type: text/plain; charset="UTF-8" >> More bad press in histonet...not sure if we should pass it on to >> Marketing?? >> @Will, Who as Teri Blaud at Holy Redeemer Hospital ? Teri is always >> always bashing us. >> Kelley Jordan >> Strategic Account Manager - SC, NC, TN and KY >> A Member of the Roche Group >> Ventana Medical Systems >> Mobile: 803.504.1135 >> Customer/Technical Support: 1.800.227.2155 >> kelley.jordan at roche.com >> www.ventana.com >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brettmc31 at comcast.net Wed Mar 6 10:18:52 2019 From: brettmc31 at comcast.net (Brett Connolly) Date: Wed, 6 Mar 2019 11:18:52 -0500 Subject: [Histonet] Roche "bashing" In-Reply-To: <1551887877.796709079@f29.my.com> References: <48E053DDF6CE074DB6A7414BA05403F8013BEE31B5@HRHEX02-HOS.holyredeemer.local> <1551887877.796709079@f29.my.com> Message-ID: As a retiree from big pharma I find your generalization to be ignorant and insulting. There are many, many researchers and laboratory personnel in big pharma that don?t fit your description, believe me! Brett Connolly, HTL(ASCP), PhD Sent from Mail for Windows 10 From: Jeff Jurczak via Histonet Sent: Wednesday, March 6, 2019 10:58 AM To: Victoria Baker Cc: histonet at lists.utsouthwestern.edu; Terri Braud; kelley.jordan at roche.com Subject: Re: [Histonet] Roche "bashing" Like all employees at big pharma companies, Kelly has been indoctrinated into believing that her company, her products, and the service rendered is perfect. To believe otherwise is a quick way to find the door out. As a corollary, there can be no problems other than user error ones.? Sent from myMail for iOS Wednesday, March 6, 2019, 7:03 AM -0600 from histonet at lists.utsouthwestern.edu : >Kelly, > >It is not bashing, it is frustration and concern. > >We need our instruments and reagents to work so that we deliver reliable >and consistent results to our pathologists. They trust our work and >dedication to them and ultimately the patient. When something like this >happens and we can't get a real resolution it undermines many things, but >trust is first for me. > >Vikki > >On Tue, Mar 5, 2019, 10:44 AM Terri Braud via Histonet < >histonet at lists.utsouthwestern.edu > wrote: > >> Such a wonderful insight into the thought process of Roche. Accused of >> bashing when I am telling the truth about failed lots when they claim the >> problem is fixed. >> If only they would put that much effort into repairing the problem and >> fostering better customer relations, than how to market their product >> around this annoying truth. >> >> Terri L. Braud, HT(ASCP) >> Anatomic Pathology Supervisor >> Laboratory >> Holy Redeemer Hospital >> 1648 Huntingdon Pike >> Meadowbrook, PA 19046 >> ph: 215-938-3689 >> fax: 215-938-3874 >> Care, Comfort, and Heal >> >> Today's Topics: >> >> 1. Re: Histonet Digest, Vol 184, Issue 1 (Jordan, Kelley) >> Message: 1 >> Date: Fri, 1 Mar 2019 13:15:58 -0500 >> From: "Jordan, Kelley" < kelley.jordan at roche.com > >> Content-Type: text/plain; charset="UTF-8" >> More bad press in histonet...not sure if we should pass it on to >> Marketing?? >> @Will, Who as Teri Blaud at Holy Redeemer Hospital ? Teri is always >> always bashing us. >> Kelley Jordan >> Strategic Account Manager - SC, NC, TN and KY >> A Member of the Roche Group >> Ventana Medical Systems >> Mobile: 803.504.1135 >> Customer/Technical Support: 1.800.227.2155 >> kelley.jordan at roche.com >> www.ventana.com >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas at biopath.org Wed Mar 6 10:53:57 2019 From: plucas at biopath.org (Paula) Date: Wed, 6 Mar 2019 08:53:57 -0800 Subject: [Histonet] Frozen Gel Pack with paraffin block for shipping? Message-ID: <002c01d4d43d$31c848d0$9558da70$@biopath.org> Hello, This question has come up here at work a few times. Apparently, the department does different protocols, depending on who is doing the job of shipping out a paraffin block for additional testing. Our transcription department handles this and I was asked to look into it so I can submit a protocol for them to follow. Is there an industry standard to follow? I'm leaning towards always putting in an frozen gel pack during the warmer months inside the shipping container to avoid any melted blocks. I know the melting point is somewhere around 136F (58C), but I still want to ensure the block doesn't get warped or altered in any way because of the heat. Plus, we shouldn't have to check the weather before sending out and I think it just should be our standard protocol. What are your thoughts and if there is a standard, please share that with me. Thank you very much, Paula Lab Manager From jaylundgren at gmail.com Wed Mar 6 12:15:14 2019 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 6 Mar 2019 12:15:14 -0600 Subject: [Histonet] Frozen Gel Pack with paraffin block for shipping? In-Reply-To: <002c01d4d43d$31c848d0$9558da70$@biopath.org> References: <002c01d4d43d$31c848d0$9558da70$@biopath.org> Message-ID: That's awesome because the block is already chilled for you when you get it, so it saves time! JK, but I think it is necessary when you send a block to Texas in August. If you're going to have a protocol, it's best to just leave it that way all the time, even if you're sending a block to Buffalo in Jan. That way someone won't forget the cold pack in July. On Wed, Mar 6, 2019 at 11:12 AM Paula via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello, > > > > This question has come up here at work a few times. Apparently, the > department does different protocols, depending on who is doing the job of > shipping out a paraffin block for additional testing. Our transcription > department handles this and I was asked to look into it so I can submit a > protocol for them to follow. > > > > Is there an industry standard to follow? I'm leaning towards always > putting > in an frozen gel pack during the warmer months inside the shipping > container > to avoid any melted blocks. I know the melting point is somewhere around > 136F (58C), but I still want to ensure the block doesn't get warped or > altered in any way because of the heat. Plus, we shouldn't have to check > the > weather before sending out and I think it just should be our standard > protocol. > > > > What are your thoughts and if there is a standard, please share that with > me. > > > > Thank you very much, > > Paula > > Lab Manager > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From pruegghm at hotmail.com Wed Mar 6 15:40:59 2019 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Wed, 6 Mar 2019 21:40:59 +0000 Subject: [Histonet] Frozen Gel Pack with paraffin block for shipping? In-Reply-To: <002c01d4d43d$31c848d0$9558da70$@biopath.org> References: <002c01d4d43d$31c848d0$9558da70$@biopath.org> Message-ID: whether I used an ice pack or not, I always bagged each block in it's own individual container/plastic bag, etc., just in case there was melting the tissue could possibly be recovered. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Paula Sent: Wednesday, March 6, 2019 9:53 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Frozen Gel Pack with paraffin block for shipping? Hello, This question has come up here at work a few times. Apparently, the department does different protocols, depending on who is doing the job of shipping out a paraffin block for additional testing. Our transcription department handles this and I was asked to look into it so I can submit a protocol for them to follow. Is there an industry standard to follow? I'm leaning towards always putting in an frozen gel pack during the warmer months inside the shipping container to avoid any melted blocks. I know the melting point is somewhere around 136F (58C), but I still want to ensure the block doesn't get warped or altered in any way because of the heat. Plus, we shouldn't have to check the weather before sending out and I think it just should be our standard protocol. What are your thoughts and if there is a standard, please share that with me. Thank you very much, Paula Lab Manager From jsaundersht at yahoo.com Wed Mar 6 17:55:57 2019 From: jsaundersht at yahoo.com (Jennifer Saunders) Date: Wed, 6 Mar 2019 23:55:57 +0000 (UTC) Subject: [Histonet] Roche "bashing" In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F8013BEE31B5@HRHEX02-HOS.holyredeemer.local> <1551887877.796709079@f29.my.com> Message-ID: <628441821.1323841.1551916557768@mail.yahoo.com> The dispenser problem have been around long before Roche entered the picture. At least a problem since 1997.. Ventana usto be really good about replacing leaky dispensers back then. If they or Roche had ever taken the problem seriously enough to actually fix the problem nobody would be doing any Roche bashing!!!Jen Saunders HT (ASCP)Portland Oregon? Sent from Yahoo Mail for iPhone On Wednesday, March 6, 2019, 8:18 AM, Brett Connolly via Histonet wrote: As a retiree from big pharma I find your generalization to be ignorant and insulting. There are many, many researchers and laboratory personnel in big pharma that don?t fit your description, believe me! Brett Connolly, HTL(ASCP), PhD Sent from Mail for Windows 10 From: Jeff Jurczak via Histonet Sent: Wednesday, March 6, 2019 10:58 AM To: Victoria Baker Cc: histonet at lists.utsouthwestern.edu; Terri Braud; kelley.jordan at roche.com Subject: Re: [Histonet] Roche "bashing" Like all employees at big pharma companies, Kelly has been indoctrinated into believing that her company, her products, and the service rendered is perfect. To believe otherwise is a quick way to find the door out. As a corollary, there can be no problems other than user error ones.? Sent from myMail for iOS Wednesday, March 6, 2019, 7:03 AM -0600 from histonet at lists.utsouthwestern.edu? : >Kelly, > >It is not bashing, it is frustration and concern. > >We need our instruments and reagents to work so that we deliver reliable >and consistent results to our pathologists.? They trust our work and >dedication to them and ultimately the patient.? When something like this >happens and we can't get a real resolution it undermines many things, but >trust is first for me. > >Vikki > >On Tue, Mar 5, 2019, 10:44 AM Terri Braud via Histonet < >histonet at lists.utsouthwestern.edu > wrote: > >> Such a wonderful insight into the thought process of Roche.? Accused of >> bashing when I am telling the truth about failed lots when they claim the >> problem is fixed. >> If only they would put that much effort into repairing the problem and >> fostering better customer relations, than how to market their product >> around this annoying truth. >> >> Terri L. Braud, HT(ASCP) >> Anatomic Pathology Supervisor >> Laboratory >> Holy Redeemer Hospital >> 1648 Huntingdon Pike >> Meadowbrook, PA 19046 >> ph: 215-938-3689 >> fax: 215-938-3874 >> Care, Comfort, and Heal >> >> Today's Topics: >> >>? ? 1. Re: Histonet Digest, Vol 184, Issue 1 (Jordan, Kelley) >> Message: 1 >> Date: Fri, 1 Mar 2019 13:15:58 -0500 >> From: "Jordan, Kelley" < kelley.jordan at roche.com > >> Content-Type: text/plain; charset="UTF-8" >> More bad press in histonet...not sure if we should pass it on to >> Marketing?? >> @Will, Who as Teri Blaud at? Holy Redeemer Hospital ? Teri is always >> always bashing us. >> Kelley Jordan >> Strategic Account Manager - SC, NC, TN and KY >> A Member of the Roche Group >> Ventana Medical Systems >> Mobile:? 803.504.1135 >> Customer/Technical Support: 1.800.227.2155 >>? kelley.jordan at roche.com >>? www.ventana.com >> >> _______________________________________________ >> Histonet mailing list >>? Histonet at lists.utsouthwestern.edu >>? http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >_______________________________________________ >Histonet mailing list >Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Wed Mar 6 20:55:55 2019 From: patpxs at gmail.com (P Sicurello) Date: Wed, 6 Mar 2019 18:55:55 -0800 Subject: [Histonet] Roche "bashing" In-Reply-To: <628441821.1323841.1551916557768@mail.yahoo.com> References: <48E053DDF6CE074DB6A7414BA05403F8013BEE31B5@HRHEX02-HOS.holyredeemer.local> <1551887877.796709079@f29.my.com> <628441821.1323841.1551916557768@mail.yahoo.com> Message-ID: It's not Roche "bashing", these are messages come from concerned histology professionals to inform the Histology world that there are issues with a product from a certain vendor. Born out of frustration with the vendor who does nothing about the problem and suggests that the issue lies with the user. Despite the fact that these "users" have been using the products for years, with or without issue, and they continue to use the product, because when the machines work they work very well. Now if this *were *bashing there would be comments like: "Roche doesn't care about patient's or how these test failures effect patient outcomes." or "Those in charge are nothing but a bunch of money grubbing scum suckers who only care about the bottom line." Perhaps, and this is a stretch, if one day, they or someone they love is negatively impacted because the HER2 dispenser failed (and her breast cancer diagnosis and treatment is delayed), or that a transplanted organ fails because, once again, the special stain that indicated rejection didn't work. But who I am I kidding? Sure there are lots of dedicated people in the trenches working hard to create medicines or improve testing, but they aren't the ones who are who don't care. Roche is too large to care about the outcomes of their failed testing products, we are small peanuts compared to the money they make off of pharmaceuticals. Last year the overall diagnostics section made 12,879 (CHF in millions) or $12,874 (US in millions) of which the tissue diagnostics division contributed 1,112 (CHF in millions) or $1,102 (US in millions). Compare that to their total sales of 56,846 (CHF in millions) or $56,602 (US in millions). Our little tissue diagnostics division contributes *1.9%* of their total sales and about *8.6%* to the overall diagnostics division. It's all right there in Roche's annual report, THAT'S why they ignore us. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Wed, Mar 6, 2019 at 3:56 PM Jennifer Saunders via Histonet < histonet at lists.utsouthwestern.edu> wrote: > The dispenser problem have been around long before Roche entered the > picture. At least a problem since 1997.. Ventana usto be really good about > replacing leaky dispensers back then. If they or Roche had ever taken the > problem seriously enough to actually fix the problem nobody would be doing > any Roche bashing!!!Jen Saunders HT (ASCP)Portland Oregon > > > Sent from Yahoo Mail for iPhone > > > On Wednesday, March 6, 2019, 8:18 AM, Brett Connolly via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > As a retiree from big pharma I find your generalization to be ignorant and > insulting. There are many, many researchers and laboratory personnel in big > pharma that don?t fit your description, believe me! > > Brett Connolly, HTL(ASCP), PhD > > Sent from Mail for Windows 10 > > From: Jeff Jurczak via Histonet > Sent: Wednesday, March 6, 2019 10:58 AM > To: Victoria Baker > Cc: histonet at lists.utsouthwestern.edu; Terri Braud; > kelley.jordan at roche.com > Subject: Re: [Histonet] Roche "bashing" > > > Like all employees at big pharma companies, Kelly has been indoctrinated > into believing that her company, her products, and the service rendered is > perfect. To believe otherwise is a quick way to find the door out. As a > corollary, there can be no problems other than user error ones. > > > > Sent from myMail for iOS > > > Wednesday, March 6, 2019, 7:03 AM -0600 from > histonet at lists.utsouthwestern.edu : > >Kelly, > > > >It is not bashing, it is frustration and concern. > > > >We need our instruments and reagents to work so that we deliver reliable > >and consistent results to our pathologists. They trust our work and > >dedication to them and ultimately the patient. When something like this > >happens and we can't get a real resolution it undermines many things, but > >trust is first for me. > > > >Vikki > > > >On Tue, Mar 5, 2019, 10:44 AM Terri Braud via Histonet < > >histonet at lists.utsouthwestern.edu > wrote: > > > >> Such a wonderful insight into the thought process of Roche. Accused of > >> bashing when I am telling the truth about failed lots when they claim > the > >> problem is fixed. > >> If only they would put that much effort into repairing the problem and > >> fostering better customer relations, than how to market their product > >> around this annoying truth. > >> > >> Terri L. Braud, HT(ASCP) > >> Anatomic Pathology Supervisor > >> Laboratory > >> Holy Redeemer Hospital > >> 1648 Huntingdon Pike > >> Meadowbrook, PA 19046 > >> ph: 215-938-3689 > >> fax: 215-938-3874 > >> Care, Comfort, and Heal > >> > >> Today's Topics: > >> > >> 1. Re: Histonet Digest, Vol 184, Issue 1 (Jordan, Kelley) > >> Message: 1 > >> Date: Fri, 1 Mar 2019 13:15:58 -0500 > >> From: "Jordan, Kelley" < kelley.jordan at roche.com > > >> Content-Type: text/plain; charset="UTF-8" > >> More bad press in histonet...not sure if we should pass it on to > >> Marketing?? > >> @Will, Who as Teri Blaud at Holy Redeemer Hospital ? Teri is always > >> always bashing us. > >> Kelley Jordan > >> Strategic Account Manager - SC, NC, TN and KY > >> A Member of the Roche Group > >> Ventana Medical Systems > >> Mobile: 803.504.1135 > >> Customer/Technical Support: 1.800.227.2155 > >> kelley.jordan at roche.com > >> www.ventana.com > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >_______________________________________________ > >Histonet mailing list > >Histonet at lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From paula at excaliburpathology.com Wed Mar 6 21:28:18 2019 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Thu, 7 Mar 2019 03:28:18 +0000 (UTC) Subject: [Histonet] Roche "bashing" In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F8013BEE31B5@HRHEX02-HOS.holyredeemer.local> <1551887877.796709079@f29.my.com> <628441821.1323841.1551916557768@mail.yahoo.com> Message-ID: <1591231758.729380.1551929298705@mail.yahoo.com> Yep. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953www.excaliburpathology.com On Wednesday, March 6, 2019, 9:14:39 PM CST, P Sicurello via Histonet wrote: It's not Roche "bashing", these are messages come from concerned histology professionals to inform the Histology world that there are issues with a product from a certain vendor.? Born out of frustration with the vendor who does nothing about the problem and suggests that the issue lies with the user.? Despite the fact that these "users" have been using the products for years, with or without issue, and they continue to use the product, because when the machines work they work very well. Now if this *were *bashing there would be comments like:? "Roche doesn't care about patient's or how these test failures effect patient outcomes." or? "Those in charge are nothing but a bunch of money grubbing scum suckers who only care about the bottom line." Perhaps, and this is a stretch, if one day, they or someone they love is negatively impacted because the HER2 dispenser failed (and her breast cancer diagnosis and treatment is delayed), or that a transplanted organ fails because, once again, the special stain that indicated rejection didn't work.? But who I am I kidding?? Sure there are lots of dedicated people in the trenches working hard to create medicines or improve testing, but they aren't the ones who are who don't care. Roche is too large to care about the outcomes of their failed testing products, we are small peanuts compared to the money they make off of pharmaceuticals.? Last year the overall diagnostics section made 12,879 (CHF in millions) or $12,874 (US in millions) of which the tissue diagnostics division contributed? 1,112 (CHF in millions) or $1,102 (US in millions). Compare that to their total sales of 56,846 (CHF in millions) or $56,602 (US in millions).? Our little tissue diagnostics division contributes *1.9%* of their total sales and about *8.6%* to the overall diagnostics division.? It's all right there in Roche's annual report, THAT'S why they ignore us. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer. On Wed, Mar 6, 2019 at 3:56 PM Jennifer Saunders via Histonet < histonet at lists.utsouthwestern.edu> wrote: > The dispenser problem have been around long before Roche entered the > picture. At least a problem since 1997.. Ventana usto be really good about > replacing leaky dispensers back then. If they or Roche had ever taken the > problem seriously enough to actually fix the problem nobody would be doing > any Roche bashing!!!Jen Saunders HT (ASCP)Portland Oregon > > > Sent from Yahoo Mail for iPhone > > > On Wednesday, March 6, 2019, 8:18 AM, Brett Connolly via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > As a retiree from big pharma I find your generalization to be ignorant and > insulting. There are many, many researchers and laboratory personnel in big > pharma that don?t fit your description, believe me! > > Brett Connolly, HTL(ASCP), PhD > > Sent from Mail for Windows 10 > > From: Jeff Jurczak via Histonet > Sent: Wednesday, March 6, 2019 10:58 AM > To: Victoria Baker > Cc: histonet at lists.utsouthwestern.edu; Terri Braud; > kelley.jordan at roche.com > Subject: Re: [Histonet] Roche "bashing" > > > Like all employees at big pharma companies, Kelly has been indoctrinated > into believing that her company, her products, and the service rendered is > perfect. To believe otherwise is a quick way to find the door out. As a > corollary, there can be no problems other than user error ones. > > > > Sent from myMail for iOS > > > Wednesday, March 6, 2019, 7:03 AM -0600 from > histonet at lists.utsouthwestern.edu? : > >Kelly, > > > >It is not bashing, it is frustration and concern. > > > >We need our instruments and reagents to work so that we deliver reliable > >and consistent results to our pathologists.? They trust our work and > >dedication to them and ultimately the patient.? When something like this > >happens and we can't get a real resolution it undermines many things, but > >trust is first for me. > > > >Vikki > > > >On Tue, Mar 5, 2019, 10:44 AM Terri Braud via Histonet < > >histonet at lists.utsouthwestern.edu > wrote: > > > >> Such a wonderful insight into the thought process of Roche.? Accused of > >> bashing when I am telling the truth about failed lots when they claim > the > >> problem is fixed. > >> If only they would put that much effort into repairing the problem and > >> fostering better customer relations, than how to market their product > >> around this annoying truth. > >> > >> Terri L. Braud, HT(ASCP) > >> Anatomic Pathology Supervisor > >> Laboratory > >> Holy Redeemer Hospital > >> 1648 Huntingdon Pike > >> Meadowbrook, PA 19046 > >> ph: 215-938-3689 > >> fax: 215-938-3874 > >> Care, Comfort, and Heal > >> > >> Today's Topics: > >> > >>? ? 1. Re: Histonet Digest, Vol 184, Issue 1 (Jordan, Kelley) > >> Message: 1 > >> Date: Fri, 1 Mar 2019 13:15:58 -0500 > >> From: "Jordan, Kelley" < kelley.jordan at roche.com > > >> Content-Type: text/plain; charset="UTF-8" > >> More bad press in histonet...not sure if we should pass it on to > >> Marketing?? > >> @Will, Who as Teri Blaud at? Holy Redeemer Hospital ? Teri is always > >> always bashing us. > >> Kelley Jordan > >> Strategic Account Manager - SC, NC, TN and KY > >> A Member of the Roche Group > >> Ventana Medical Systems > >> Mobile:? 803.504.1135 > >> Customer/Technical Support: 1.800.227.2155 > >>? kelley.jordan at roche.com > >>? www.ventana.com > >> > >> _______________________________________________ > >> Histonet mailing list > >>? Histonet at lists.utsouthwestern.edu > >>? http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >_______________________________________________ > >Histonet mailing list > >Histonet at lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Thu Mar 7 18:11:36 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 8 Mar 2019 00:11:36 +0000 Subject: [Histonet] Really Small acid cabinet? Message-ID: Does anyone know of an acid cabinet for just a few 500ml bottles? All I can find fit 6 1-liter bottles, no smaller. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From wahlfeld at ptd.net Thu Mar 7 18:28:34 2019 From: wahlfeld at ptd.net (William Ahlfeld) Date: Thu, 7 Mar 2019 19:28:34 -0500 Subject: [Histonet] Really Small acid cabinet? Message-ID: Check out Uline part #H-4174 Sent from my iPad From melissa at alliedsearchpartners.com Fri Mar 8 10:59:45 2019 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Fri, 8 Mar 2019 16:59:45 +0000 Subject: [Histonet] NY-Anatomic Pathology Job Openings Message-ID: Happy Friday, Just spreading the word on some new openings I have in New York. Please contact me directly for more details/job description. Long Island Area (New Hyde Park) 1. Grossing Histotech/Pathologist Assistant (Tuesday-Saturday 10am-6:30pm)-New York License Eligibility Bronx Area 1. Pathologist Assistant/Grossing Histotech (Monday-Friday 3pm-11:30pm)- Must have New York Clinical Laboratory License or PA license 1. Histotech (Monday-Friday 2pm-10:30pm)-Must have New York Clinical Laboratory License in histology or clinical laboratory technology Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 Direct Line: 407.413.9117 From melissa at alliedsearchpartners.com Fri Mar 8 11:10:54 2019 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Fri, 8 Mar 2019 17:10:54 +0000 Subject: [Histonet] Typo-NY Anatomic Pathology Jobs Message-ID: Sorry there was a typo on location of the last email. Please see correct version of my last email. Happy Friday, Just spreading the word on some new openings I have in New York. Please contact me directly for more details/job description. Long Island Area (New Hyde Park) Grossing Histotech/Pathologist Assistant (Tuesday-Saturday 10am-6:30pm)-New York License Eligibility Bronx Area Pathologist Assistant/Grossing Histotech (Monday-Friday 3pm-11:30pm)- Must have New York Clinical Laboratory License or PA license Histotech (Monday-Friday 2pm-10:30pm)-Must have New York Clinical Laboratory License in histology or clinical laboratory technology Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 Direct Line: 407.413.9117 F: 888.388.7572 From mim9060 at nyp.org Fri Mar 8 12:11:13 2019 From: mim9060 at nyp.org (Michele Maimone-Schoen) Date: Fri, 8 Mar 2019 18:11:13 +0000 Subject: [Histonet] [EXTERNAL] Histonet Digest, Vol 184, Issue 7 In-Reply-To: References: Message-ID: <56f9fb62f10a402692a05b1082760f43@nyp.org> There are openings at New York Presbyterian Hospital/Weill Cornell Division, 68th Street, NYC: Histotech (Monday-Friday 12pm-8pm)-Must have New York Clinical Laboratory License in histology or clinical laboratory technology Histotech (Tuesday-Saturday, day flex)-Must have New York Clinical Laboratory License in histology or clinical laboratory technology Please contact me at 212-746-2633 for more details. Thanks -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Friday, March 8, 2019 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 184, Issue 7 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=apLCJo22jkVRpFivmRGGHnRn85FoLi_g9mEBSlVKwRY&r=glcV8HADLxkuv0pL9qAWXQ&m=3W-b5InoPkQaMP70hQa5VvzY223OqRPC4Rw-DPRnwPY&s=YffIT9A3-Mqxmp1Gz-bp-IPoYpTpvbQXp7xM6lxlMz4&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Really Small acid cabinet? (Morken, Timothy) 2. Re: Really Small acid cabinet? (William Ahlfeld) 3. NY-Anatomic Pathology Job Openings (Melissa Owens) 4. Typo-NY Anatomic Pathology Jobs (Melissa Owens) ---------------------------------------------------------------------- Message: 1 Date: Fri, 8 Mar 2019 00:11:36 +0000 From: "Morken, Timothy" To: Histonet Subject: [Histonet] Really Small acid cabinet? Message-ID: Content-Type: text/plain; charset="us-ascii" Does anyone know of an acid cabinet for just a few 500ml bottles? All I can find fit 6 1-liter bottles, no smaller. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center ------------------------------ Message: 2 Date: Thu, 7 Mar 2019 19:28:34 -0500 From: William Ahlfeld To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Really Small acid cabinet? Message-ID: Content-Type: text/plain; charset=us-ascii Check out Uline part #H-4174 Sent from my iPad ------------------------------ Message: 3 Date: Fri, 8 Mar 2019 16:59:45 +0000 From: Melissa Owens To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] NY-Anatomic Pathology Job Openings Message-ID: Content-Type: text/plain; charset="us-ascii" Happy Friday, Just spreading the word on some new openings I have in New York. Please contact me directly for more details/job description. Long Island Area (New Hyde Park) 1. Grossing Histotech/Pathologist Assistant (Tuesday-Saturday 10am-6:30pm)-New York License Eligibility Bronx Area 1. Pathologist Assistant/Grossing Histotech (Monday-Friday 3pm-11:30pm)- Must have New York Clinical Laboratory License or PA license 1. Histotech (Monday-Friday 2pm-10:30pm)-Must have New York Clinical Laboratory License in histology or clinical laboratory technology Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 Direct Line: 407.413.9117 ------------------------------ Message: 4 Date: Fri, 8 Mar 2019 17:10:54 +0000 From: Melissa Owens To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Typo-NY Anatomic Pathology Jobs Message-ID: Content-Type: text/plain; charset="us-ascii" Sorry there was a typo on location of the last email. Please see correct version of my last email. Happy Friday, Just spreading the word on some new openings I have in New York. Please contact me directly for more details/job description. Long Island Area (New Hyde Park) Grossing Histotech/Pathologist Assistant (Tuesday-Saturday 10am-6:30pm)-New York License Eligibility Bronx Area Pathologist Assistant/Grossing Histotech (Monday-Friday 3pm-11:30pm)- Must have New York Clinical Laboratory License or PA license Histotech (Monday-Friday 2pm-10:30pm)-Must have New York Clinical Laboratory License in histology or clinical laboratory technology Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 Direct Line: 407.413.9117 F: 888.388.7572 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=apLCJo22jkVRpFivmRGGHnRn85FoLi_g9mEBSlVKwRY&r=glcV8HADLxkuv0pL9qAWXQ&m=3W-b5InoPkQaMP70hQa5VvzY223OqRPC4Rw-DPRnwPY&s=YffIT9A3-Mqxmp1Gz-bp-IPoYpTpvbQXp7xM6lxlMz4&e= ------------------------------ End of Histonet Digest, Vol 184, Issue 7 **************************************** ---------------------------------------------------------------------- This electronic message is intended to be for the use only of the named recipient, and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. Thank you. code:d34y From TNMayer at mdanderson.org Fri Mar 8 13:32:21 2019 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Fri, 8 Mar 2019 19:32:21 +0000 Subject: [Histonet] Air pocket in paraffin blocks Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC883ADD75B2@D1PWPEXMBX06.mdanderson.edu> Vikki, I teach students that they should not have any air bubbles in their blocks. This means those under the lip of the cassette as well as surrounding the tissues. Air bubbles can cause instability in the block especially if the tissue is hard or very small. When they go to a clinical rotation no preceptor checks their blocks as closely as I do, but they can get a 'perfect' block without much effort. Our tissues and cassettes have mostly been donated, so the quality may not be as optimal as that in a clinical lab, so if they can get a good block embedded with me, they can do it anywhere. It is actually a scored item on our graded checklist, along with the overall embedded time. You might want to do an in-service on air bubbles and give them a CEU for it. Hope this helps. Toysha N. Mayer D.H.Sc., MBA, HT(ASCP) Assistant Professor/Associate Program Director HTL Program MD Anderson School of Health Professions 713.563.3481 tnmayer at mdanderson.org Message: 1 Date: Fri, 22 Feb 2019 18:32:41 +0000 From: "deGuzman, Jose R" To: Victoria Baker , "histonet at lists.utsouthwestern.edu" Subject: Message-ID: Content-Type: text/plain; charset="utf-8" Hello Vikki, What you are experiencing is the lack of quality feedback. The "experienced" techs may have never been told that their quality needs to improve due to complacency or management is afraid they might leave. This gets worse if they have gotten away with producing poor quality for a long time and nobody has provided much needed feedback to them. You may have an uphill battle ahead of you but very much worth taking. Jose -----Original Message----- From: Victoria Baker Sent: Thursday, February 21, 2019 5:49 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Air pocket in paraffin blocks Hi, When I was trained to do embedding there were many things that the professor stressed to me need to be done in order to have the tissue block acceptable for sectioning. One of these was air bubbles. Recently we had a new tech embed a derm block that had an bubble that was pretty big. The other (experienced) techs didn't think anything of it either and sectioned it. When I got the block for IHC screening I made a QA form stating that the block should have been re-embedded before giving it to be sectioned, or when the first tech sectioned it could have repaired the block or melted it down. This air bubble was big enough to be seen so I don't think it could have been missed - unless the block wasn't checked right after embedding. What has me a little upset is that no one seemed to care about this. I would really appreciate some feedback about this from other people. Thanks in advance. Vikki The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From bakevictoria at gmail.com Fri Mar 8 14:00:33 2019 From: bakevictoria at gmail.com (Victoria Baker) Date: Fri, 8 Mar 2019 15:00:33 -0500 Subject: [Histonet] Air pocket in paraffin blocks In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC883ADD75B2@D1PWPEXMBX06.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC883ADD75B2@D1PWPEXMBX06.mdanderson.edu> Message-ID: Thank you everyone for your responses. It is very much appreciated. It is an up hill issue as most of the technical staff have been here and maybe one other lab in their entire career and "no one's complained before". I'd like that phrase and "that's the way we've always done it" stricken from all languages and any alien dialects we might ever encounter in our lives. Happy Friday and have a great weekend! Vikki On Fri, Mar 8, 2019, 2:38 PM Mayer,Toysha N via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Vikki, > > I teach students that they should not have any air bubbles in their > blocks. This means those under the lip of the cassette as well as > surrounding the tissues. Air bubbles can cause instability in the block > especially if the tissue is hard or very small. When they go to a clinical > rotation no preceptor checks their blocks as closely as I do, but they can > get a 'perfect' block without much effort. Our tissues and cassettes have > mostly been donated, so the quality may not be as optimal as that in a > clinical lab, so if they can get a good block embedded with me, they can do > it anywhere. > It is actually a scored item on our graded checklist, along with the > overall embedded time. > You might want to do an in-service on air bubbles and give them a CEU for > it. > > Hope this helps. > > Toysha N. Mayer D.H.Sc., MBA, HT(ASCP) > Assistant Professor/Associate Program Director > HTL Program > MD Anderson School of Health Professions > 713.563.3481 > tnmayer at mdanderson.org > > > > Message: 1 > Date: Fri, 22 Feb 2019 18:32:41 +0000 > From: "deGuzman, Jose R" > To: Victoria Baker , > "histonet at lists.utsouthwestern.edu" > > Subject: > Message-ID: > < > BY2PR13MB06966AAED227C932C3335091967F0 at BY2PR13MB0696.namprd13.prod.outlook.com > > > > Content-Type: text/plain; charset="utf-8" > > Hello Vikki, > > What you are experiencing is the lack of quality feedback. The > "experienced" techs may have never been told that their quality needs to > improve due to complacency or management is afraid they might leave. This > gets worse if they have gotten away with producing poor quality for a long > time and nobody has provided much needed feedback to them. You may have an > uphill battle ahead of you but very much worth taking. > > Jose > > -----Original Message----- > From: Victoria Baker > Sent: Thursday, February 21, 2019 5:49 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Air pocket in paraffin blocks > > Hi, > > When I was trained to do embedding there were many things that the > professor stressed to me need to be done in order to have the tissue block > acceptable for sectioning. One of these was air bubbles. > > Recently we had a new tech embed a derm block that had an bubble that was > pretty big. The other (experienced) techs didn't think anything of it > either and sectioned it. When I got the block for IHC screening I made a > QA form stating that the block should have been re-embedded before giving > it to be sectioned, or when the first tech sectioned it could have repaired > the block or melted it down. This air bubble was big enough to be seen so > I don't think it could have been missed - unless the block wasn't checked > right after embedding. > > What has me a little upset is that no one seemed to care about this. > > I would really appreciate some feedback about this from other people. > > Thanks in advance. > > Vikki > > > The information contained in this e-mail message may be privileged, > confidential, and/or protected from disclosure. This e-mail message may > contain protected health information (PHI); dissemination of PHI should > comply with applicable federal and state laws. If you are not the intended > recipient, or an authorized representative of the intended recipient, any > further review, disclosure, use, dissemination, distribution, or copying of > this message or any attachment (or the information contained therein) is > strictly prohibited. If you think that you have received this e-mail > message in error, please notify the sender by return e-mail and delete all > references to it and its contents from your systems. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kelly_colpitts at hotmail.com Sat Mar 9 18:01:38 2019 From: kelly_colpitts at hotmail.com (Kelly Pairan) Date: Sun, 10 Mar 2019 00:01:38 +0000 Subject: [Histonet] Histotechnician Message-ID: We are looking for an experienced full-time histotechnician at Nationwide Children's Hospital. ASCP certified is preferred but if the candidate is not certified, they must become certified within 3 years. For more information about this position, see the following link. https://external-nationwidechildrens.icims.com/jobs/15551/histotechnician---anatomic-pathology/job Have a great day! Kelly Get Outlook for Android From LRaff at uropartners.com Mon Mar 11 10:23:03 2019 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 11 Mar 2019 15:23:03 +0000 Subject: [Histonet] Keeping HIPAA in mind--it relates to all of us. Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF121085A7@COLOEXCH01.uropartners.local> I haven't posted a blog for quite a while here. But this is something for all of us to remember, especially when it gets in the public eye. http://www.chicagonow.com/downsize-maybe/2019/03/hipaa/ The above is the opinion of the author and not UroPartners, LLC Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From relia1 at earthlink.net Mon Mar 11 12:28:00 2019 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 11 Mar 2019 13:28:00 -0400 Subject: [Histonet] Spring Break is almost HERE!! Message-ID: <000001d4d82f$c6ea66a0$54bf33e0$@earthlink.net> Hi Histonetters, Spring Break is Almost HERE! I can tell because it?s getting warmer and lots of new things are blooming and I?m not just talking about the weather and the flowers!! #springbreak2018 I am looking forward to beating the heat with some amazing beach days!!! So what are you doing for your Spring Break? ? Going somewhere exciting? ? A Staycation? ? Visiting Family or Friends? ? Attending a Graduation, A Wedding or Family Reunion? Visiting anyplace you might consider relocation to? If so drop me a line and let me know so I can make a note to keep you posted on positions in that area. Even if we have talked recently drop me a line and remind me!! I do have some exciting opportunities that might coincide with your plans and if not I do have new opportunities coming in DAILY!!! I have opportunities in Leadership in: ? MA ? Cape Cod ? GA ? Atlanta ? OH ? (Sales) ? WI ? Milwaukee I have opportunities for Technicians and Technologists in: ? AL----Birmingham ? TN----Chattanooga ? MD----Annapolis ? MA----Cape Cod ? MA----Springfield ? VA----Norfolk ? Senior Tech 9K sign-on bonus!! ? FL----Ft. Myers (CLIA qualified to gross) ? GA----Atlanta All of my clients offer excellent compensation, benefits and some offer relocation assistance and or sign on bonuses. All of these jobs are full time & permanent NEW POSITIONS ARE COMING IN DAILY!!! So Drop me a line and let me know where you want to go!! Histonetters, if you or anyone you know is interested in hearing more about any of these opportunities please contact me. Remember if I place someone you refer to me you will earn a referral bonus!!! Is your lab shorthanded? Did you know that if I get the job opening with your help and place someone in the position you will also earn a referral fee? Ask me how!!!! I can be reached anytime on my cell/text at 407-353-5070 or toll free at 866-607-3542 or e-mail me at relia1 at earthlink.net Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From criley at dpspa.com Mon Mar 11 13:34:10 2019 From: criley at dpspa.com (Charles Riley) Date: Mon, 11 Mar 2019 14:34:10 -0400 Subject: [Histonet] Job opening Message-ID: Hello all, We are offering a full time histotechnician position in Dover Delaware. Morning shift (start time semi negotiable). Chance to be cross trained in other departments as well as advancement to leadership roles. Seeking someone with 5+ years experience (less acceptable but not preferred) and HT or HTL certified or certification eligible. If interested please reply here and I can give you more information about the position and give you instructions on how to apply if you are interested -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From morttl at ucmail.uc.edu Tue Mar 12 13:04:31 2019 From: morttl at ucmail.uc.edu (Hopkins, Tracy (morttl)) Date: Tue, 12 Mar 2019 18:04:31 +0000 Subject: [Histonet] Histonet Digest, Vol 184, Issue 11 In-Reply-To: References: Message-ID: Thanksgivings is m.m me On Mar 12, 2019 1:01 PM, histonet-request at lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Spring Break is almost HERE!! (Pam Barker) 2. Job opening (Charles Riley) ---------------------------------------------------------------------- Message: 1 Date: Mon, 11 Mar 2019 13:28:00 -0400 From: "Pam Barker" To: "Histonetters Histonet" Subject: [Histonet] Spring Break is almost HERE!! Message-ID: <000001d4d82f$c6ea66a0$54bf33e0$@earthlink.net> Content-Type: text/plain; charset="utf-8" Hi Histonetters, Spring Break is Almost HERE! I can tell because it?s getting warmer and lots of new things are blooming and I?m not just talking about the weather and the flowers!! #springbreak2018 I am looking forward to beating the heat with some amazing beach days!!! So what are you doing for your Spring Break? ? Going somewhere exciting? ? A Staycation? ? Visiting Family or Friends? ? Attending a Graduation, A Wedding or Family Reunion? Visiting anyplace you might consider relocation to? If so drop me a line and let me know so I can make a note to keep you posted on positions in that area. Even if we have talked recently drop me a line and remind me!! I do have some exciting opportunities that might coincide with your plans and if not I do have new opportunities coming in DAILY!!! I have opportunities in Leadership in: ? MA ? Cape Cod ? GA ? Atlanta ? OH ? (Sales) ? WI ? Milwaukee I have opportunities for Technicians and Technologists in: ? AL----Birmingham ? TN----Chattanooga ? MD----Annapolis ? MA----Cape Cod ? MA----Springfield ? VA----Norfolk ? Senior Tech 9K sign-on bonus!! ? FL----Ft. Myers (CLIA qualified to gross) ? GA----Atlanta All of my clients offer excellent compensation, benefits and some offer relocation assistance and or sign on bonuses. All of these jobs are full time & permanent NEW POSITIONS ARE COMING IN DAILY!!! So Drop me a line and let me know where you want to go!! Histonetters, if you or anyone you know is interested in hearing more about any of these opportunities please contact me. Remember if I place someone you refer to me you will earn a referral bonus!!! Is your lab shorthanded? Did you know that if I get the job opening with your help and place someone in the position you will also earn a referral fee? Ask me how!!!! I can be reached anytime on my cell/text at 407-353-5070 or toll free at 866-607-3542 or e-mail me at relia1 at earthlink.net Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 2 Date: Mon, 11 Mar 2019 14:34:10 -0400 From: Charles Riley To: Histo List Subject: [Histonet] Job opening Message-ID: Content-Type: text/plain; charset="UTF-8" Hello all, We are offering a full time histotechnician position in Dover Delaware. Morning shift (start time semi negotiable). Chance to be cross trained in other departments as well as advancement to leadership roles. Seeking someone with 5+ years experience (less acceptable but not preferred) and HT or HTL certified or certification eligible. If interested please reply here and I can give you more information about the position and give you instructions on how to apply if you are interested -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 184, Issue 11 ***************************************** From b-frederick at northwestern.edu Wed Mar 13 11:11:09 2019 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Wed, 13 Mar 2019 16:11:09 +0000 Subject: [Histonet] P16 Message-ID: Who is the best and current supplier for p16? We were using biocare,but evidently it is no longer available from them. And no Santa Cruz per my IHC tech. Thanks, Bernice Bernice Frederick Pathology Core Facility Robert H. Lurie Cancer Center 710 North Fairbanks Court Olson 8-421 312-503-3723 b-frederick at northwestern.edu From criley at dpspa.com Wed Mar 13 12:23:48 2019 From: criley at dpspa.com (Charles Riley) Date: Wed, 13 Mar 2019 13:23:48 -0400 Subject: [Histonet] P16 In-Reply-To: References: Message-ID: We by predilute from BioGenix On Wed, Mar 13, 2019 at 12:27 PM Bernice Frederick via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Who is the best and current supplier for p16? We were using biocare,but > evidently it is no longer available from them. And no Santa Cruz per my IHC > tech. > Thanks, > Bernice > > Bernice Frederick > Pathology Core Facility > Robert H. Lurie Cancer Center > 710 North Fairbanks Court > Olson 8-421 > 312-503-3723 > b-frederick at northwestern.edu > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From BAMoe at gundersenhealth.org Wed Mar 13 12:43:51 2019 From: BAMoe at gundersenhealth.org (Moe, Barbi A) Date: Wed, 13 Mar 2019 17:43:51 +0000 Subject: [Histonet] ER/PR controls Message-ID: For anyone making in-house ER/PR controls could you comment on what tissue you have in your control block -- one piece of positive tumor regardless of reactivity strength 1+, 2+, 3+ (weak, medium, strong) or multiple pieces to represent each level of reactivity? Also, does anyone order commercially their ER/PR controls to achieve this level of representation in their control block? Any thoughts are appreciated - thank you. Barb Moe Gundersen Health System La Crosse WI bamoe at gundersenhealth.org From cforster at umn.edu Wed Mar 13 12:48:12 2019 From: cforster at umn.edu (Colleen Forster) Date: Wed, 13 Mar 2019 12:48:12 -0500 Subject: [Histonet] ER/PR controls In-Reply-To: References: Message-ID: Barbi, When we designed ours we had multiple samples that included: neg, 1+,2+, 3+, 4+ both ER and PR and a second one had those plus the Her2 the same... C On Wed, Mar 13, 2019 at 12:44 PM Moe, Barbi A via Histonet < histonet at lists.utsouthwestern.edu> wrote: > For anyone making in-house ER/PR controls could you comment on what tissue > you have in your control block -- one piece of positive tumor regardless of > reactivity strength 1+, 2+, 3+ (weak, medium, strong) or multiple pieces to > represent each level of reactivity? > > > Also, does anyone order commercially their ER/PR controls to achieve this > level of representation in their control block? > > > Any thoughts are appreciated - thank you. > > > Barb Moe > > Gundersen Health System > > La Crosse WI > > bamoe at gundersenhealth.org > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at holml at umn.edu * From jmyers1 at aol.com Wed Mar 13 14:08:48 2019 From: jmyers1 at aol.com (Joe Myers) Date: Wed, 13 Mar 2019 15:08:48 -0400 Subject: [Histonet] p16 Message-ID: <30AFD2E8-0C7B-43EB-BBF7-98C860CF13CC@aol.com> To All Concerned Parties: Please to be advised that the antibody to p16 offered by Biocare Medical, labeled ASR, is still available (in RTU-format only). Joe Myers, M.S., CT(ASCP)QIHC Senior Technical Sales Specialist Biocare Medical ------------------------------ Message: 2 Date: Wed, 13 Mar 2019 16:11:09 +0000 From: Bernice Frederick To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] P16 Who is the best and current supplier for p16? We were using biocare,but evidently it is no longer available from them. And no Santa Cruz per my IHC tech. Thanks, Bernice Bernice Frederick Pathology Core Facility Robert H. Lurie Cancer Center 710 North Fairbanks Court Olson 8-421 312-503-3723 b-frederick at northwestern.edu From redrose297 at gmail.com Wed Mar 13 14:53:17 2019 From: redrose297 at gmail.com (warda hassan) Date: Wed, 13 Mar 2019 23:53:17 +0400 Subject: [Histonet] Omnis Message-ID: Dear all Could anyone share their experience with dako omnis immune stainer from turn around time, stain quality, FDA approved antibodies & kits, problems ( technical or mechanical) Thanking all in advance for their contributions as it will be very helpful in choosing it against Leica Bond. Thank you Wishing all a good day From aperl at cmmedical.com Wed Mar 13 15:08:14 2019 From: aperl at cmmedical.com (Perl , Alison) Date: Wed, 13 Mar 2019 20:08:14 +0000 Subject: [Histonet] stainer v. stainer In-Reply-To: <72c4f07a3c31479c8f23298168c2aabb@MK-EXMB02.mkmg.com> References: <72c4f07a3c31479c8f23298168c2aabb@MK-EXMB02.mkmg.com> Message-ID: <96b98f0b1a6746e0989160ba4d1aa298@MK-EXMB02.mkmg.com> Hi all We are getting ready to purchase a new H&E stainer/coverslipper, and are considering the Sakura Prisma Plus (tape) and the Leica Spectra (glass). Does anyone have good or bad feedback on either instrument, and/or tape v. glass? We've always had glass, but of course the coverslippers need more maintenance, take longer to dry, more expensive than tape, etc etc. So we are very interested in tape, but still a little hesitant about the old problems of yellowing and peeling after 10+ years. Since we're in NY, we have to keep all slides for 20 years.... Any thoughts are appreciated! Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical 110 South Bedford Rd Mount Kisco, NY 10549 (914) 302-8424 aperl at cmmedical.com www.caremountmedical.com This email and its attachments, if any, may contain confidential or proprietary information and are intended solely for authorized use by the intended recipient(s) only. Any other use of this email is prohibited. If you have received this email in error, you are hereby notified that any retention, disclosure, copying, forwarding, distribution (in whole or in part and whether electronically, written and/or orally) and/or taking of any action in reliance on this email, its contents and/or any attachments thereto is strictly prohibited. If you received this email in error, please notify the sender by replying to this message and permanently delete this email, and any attachments thereto, from your system immediately. From Karen.Heckford at DignityHealth.org Thu Mar 14 06:59:16 2019 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Thu, 14 Mar 2019 11:59:16 +0000 Subject: [Histonet] Surgical Hardware Message-ID: Good Morning, We have an abundance of surgical hardware we need to get rid of from our Pathology Department. How do you get rid of your surgical hardware? Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you From mkent at dermpathlab.com Thu Mar 14 07:44:54 2019 From: mkent at dermpathlab.com (Michael Kent) Date: Thu, 14 Mar 2019 12:44:54 +0000 Subject: [Histonet] P16 In-Reply-To: References: Message-ID: You might check Cancer Diagnostics, inc.. Their rep told us they have an IVD p16. Best, Mike -----Original Message----- From: Charles Riley via Histonet Sent: Wednesday, March 13, 2019 1:24 PM To: Bernice Frederick Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] P16 We by predilute from BioGenix On Wed, Mar 13, 2019 at 12:27 PM Bernice Frederick via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Who is the best and current supplier for p16? We were using > biocare,but evidently it is no longer available from them. And no > Santa Cruz per my IHC tech. > Thanks, > Bernice > > Bernice Frederick > Pathology Core Facility > Robert H. Lurie Cancer Center > 710 North Fairbanks Court > Olson 8-421 > 312-503-3723 > b-frederick at northwestern.edu > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bliven.laura at marshfieldclinic.org Thu Mar 14 08:58:17 2019 From: bliven.laura at marshfieldclinic.org (Bliven, Laura M) Date: Thu, 14 Mar 2019 13:58:17 +0000 Subject: [Histonet] Artifact IHC Message-ID: I have seen an artifact on an Estrogen IHC slide, although rare, our pathologists have also seen it on outreach slides. It appears somewhat granular and/or dot-like and it is not on the Progesterone slide. The Estrogen uses a high pH and our Progesterone does not. I assume it's caused by the high pH epitope retrieval, but would like a reference to this theory. Any ideas? Laura Bliven.laura at marshfieldclinic.org ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From tbraud at holyredeemer.com Thu Mar 14 13:50:15 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 14 Mar 2019 18:50:15 +0000 Subject: [Histonet] ER/PR Control Message-ID: <48E053DDF6CE074DB6A7414BA05403F8013BEE4CF4@HRHEX02-HOS.holyredeemer.local> Hi Barbi - We are somewhere in-between with our ER/PR Controls. When performing lot to lot validations, we use our in-house control, which only has a 0 and a 3+, BUT we also use a commercially prepared control that contains 4 cores, 0. 1+, 2+, 3+. Once the new lot of antibody has been validated against both controls, then we only use our inhouse control with the 0 and 3+. It just saves on time and money. Hope this helps, Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 2. ER/PR controls (Moe, Barbi A) 3. Re: ER/PR controls (Colleen Forster) Message: 2 Date: Wed, 13 Mar 2019 17:43:51 +0000 From: "Moe, Barbi A" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] ER/PR controls For anyone making in-house ER/PR controls could you comment on what tissue you have in your control block -- one piece of positive tumor regardless of reactivity strength 1+, 2+, 3+ (weak, medium, strong) or multiple pieces to represent each level of reactivity? Also, does anyone order commercially their ER/PR controls to achieve this level of representation in their control block? Any thoughts are appreciated - thank you. Barb Moe Gundersen Health System La Crosse WI bamoe at gundersenhealth.org From tbraud at holyredeemer.com Thu Mar 14 14:10:15 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 14 Mar 2019 19:10:15 +0000 Subject: [Histonet] Stainer vs. Stainer Message-ID: <48E053DDF6CE074DB6A7414BA05403F8013BEE4D1D@HRHEX02-HOS.holyredeemer.local> Hi Alison - I've used both stainers and like both of them a lot. Both were super reliable and easy to use. However, coverslipping is a different story. I've used both film and glass. About film - super quick, super easy, but - the purity of the xylene used to coverslip from film must be absolute. Anyone who has experienced film pulling off the slides in storage had a miniscule portion of water carried down the acohols and into the xylene. If it were glass, the process is a bit more forgiving of water contaminent. The absolute alcohols leading to the end xylenes must be kept very fresh. I kept film slides for over 20 years, no problem. If you are looking into digital pathology, I would check with vendors to see if film is acceptable. I don't know. As to coverslippers, we've been using the Sakura glass now for 10 years and love it. I can't compare it to the newer Leica Glass, but 10 years ago my techs all preferred the Sakura because it had fewer moving parts and the maintenance was easier. I hope this helps. Good Luck, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 6. stainer v. stainer (Perl , Alison) Message: 6 Date: Wed, 13 Mar 2019 20:08:14 +0000 From: "Perl , Alison" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] stainer v. stainer Hi all We are getting ready to purchase a new H&E stainer/coverslipper, and are considering the Sakura Prisma Plus (tape) and the Leica Spectra (glass). Does anyone have good or bad feedback on either instrument, and/or tape v. glass? We've always had glass, but of course the coverslippers need more maintenance, take longer to dry, more expensive than tape, etc etc. So we are very interested in tape, but still a little hesitant about the old problems of yellowing and peeling after 10+ years. Since we're in NY, we have to keep all slides for 20 years.... Any thoughts are appreciated! Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical 110 South Bedford Rd Mount Kisco, NY 10549 (914) 302-8424 aperl at cmmedical.com www.caremountmedical.com From Timothy.Morken at ucsf.edu Thu Mar 14 14:26:28 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 14 Mar 2019 19:26:28 +0000 Subject: [Histonet] tape for digital scanning...RE: Stainer vs. Stainer Message-ID: I'll note that we have used glass coverslips in histology forever but are switching to tape not for the speed (though that is appreciated) but because the tape dries instantly and can be put in a slide scanner right away, while glass slides cannot until dry enough - takes a lot longer. In fact, we were cutting tape strips down to use as coverslips for FS slides so we could scan right away for remote Dx while the OR was waiting. The taped slides scan fine and the images are fine. The primary complaint about taped slides is that the tape scratches and so makes microscopy a bit more difficult, and we pull slides constantly for various conferences. However, with scanned images it makes no difference. The slides are scanned when the tape is pristine, stored and rarely pulled again. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Terri Braud via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, March 14, 2019 12:10 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Stainer vs. Stainer Hi Alison - I've used both stainers and like both of them a lot. Both were super reliable and easy to use. However, coverslipping is a different story. I've used both film and glass. About film - super quick, super easy, but - the purity of the xylene used to coverslip from film must be absolute. Anyone who has experienced film pulling off the slides in storage had a miniscule portion of water carried down the acohols and into the xylene. If it were glass, the process is a bit more forgiving of water contaminent. The absolute alcohols leading to the end xylenes must be kept very fresh. I kept film slides for over 20 years, no problem. If you are looking into digital pathology, I would check with vendors to see if film is acceptable. I don't know. As to coverslippers, we've been using the Sakura glass now for 10 years and love it. I can't compare it to the newer Leica Glass, but 10 years ago my techs all preferred the Sakura because it had fewer moving parts and the maintenance was easier. I hope this helps. Good Luck, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 6. stainer v. stainer (Perl , Alison) Message: 6 Date: Wed, 13 Mar 2019 20:08:14 +0000 From: "Perl , Alison" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] stainer v. stainer Hi all We are getting ready to purchase a new H&E stainer/coverslipper, and are considering the Sakura Prisma Plus (tape) and the Leica Spectra (glass). Does anyone have good or bad feedback on either instrument, and/or tape v. glass? We've always had glass, but of course the coverslippers need more maintenance, take longer to dry, more expensive than tape, etc etc. So we are very interested in tape, but still a little hesitant about the old problems of yellowing and peeling after 10+ years. Since we're in NY, we have to keep all slides for 20 years.... Any thoughts are appreciated! Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical 110 South Bedford Rd Mount Kisco, NY 10549 (914) 302-8424 aperl at cmmedical.com https://protect2.fireeye.com/url?k=0a167f0a-5656125c-0a165817-0cc47adb57f0-ef5d177b74bfb01b&u=http://www.caremountmedical.com/ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang at gmx.at Thu Mar 14 14:49:02 2019 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 14 Mar 2019 20:49:02 +0100 Subject: [Histonet] Stainer vs. Stainer In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8013BEE4D1D@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8013BEE4D1D@HRHEX02-HOS.holyredeemer.local> Message-ID: <000501d4da9e$fa454a80$eecfdf80$@gmx.at> Hi Terri, Have you actually used the Spectra from Leica? This is the follower of ST5020 and CV5020. I am also interested in some feedback on this instrument. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: Terri Braud via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Donnerstag, 14. M?rz 2019 20:10 An: 'histonet at lists.utsouthwestern.edu' Betreff: [Histonet] Stainer vs. Stainer Hi Alison - I've used both stainers and like both of them a lot. Both were super reliable and easy to use. However, coverslipping is a different story. I've used both film and glass. About film - super quick, super easy, but - the purity of the xylene used to coverslip from film must be absolute. Anyone who has experienced film pulling off the slides in storage had a miniscule portion of water carried down the acohols and into the xylene. If it were glass, the process is a bit more forgiving of water contaminent. The absolute alcohols leading to the end xylenes must be kept very fresh. I kept film slides for over 20 years, no problem. If you are looking into digital pathology, I would check with vendors to see if film is acceptable. I don't know. As to coverslippers, we've been using the Sakura glass now for 10 years and love it. I can't compare it to the newer Leica Glass, but 10 years ago my techs all preferred the Sakura because it had fewer moving parts and the maintenance was easier. I hope this helps. Good Luck, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 6. stainer v. stainer (Perl , Alison) Message: 6 Date: Wed, 13 Mar 2019 20:08:14 +0000 From: "Perl , Alison" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] stainer v. stainer Hi all We are getting ready to purchase a new H&E stainer/coverslipper, and are considering the Sakura Prisma Plus (tape) and the Leica Spectra (glass). Does anyone have good or bad feedback on either instrument, and/or tape v. glass? We've always had glass, but of course the coverslippers need more maintenance, take longer to dry, more expensive than tape, etc etc. So we are very interested in tape, but still a little hesitant about the old problems of yellowing and peeling after 10+ years. Since we're in NY, we have to keep all slides for 20 years.... Any thoughts are appreciated! Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical 110 South Bedford Rd Mount Kisco, NY 10549 (914) 302-8424 aperl at cmmedical.com www.caremountmedical.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Fri Mar 15 09:22:52 2019 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 15 Mar 2019 14:22:52 +0000 (UTC) Subject: [Histonet] Stainer vs. Stainer In-Reply-To: <000501d4da9e$fa454a80$eecfdf80$@gmx.at> References: <48E053DDF6CE074DB6A7414BA05403F8013BEE4D1D@HRHEX02-HOS.holyredeemer.local> <000501d4da9e$fa454a80$eecfdf80$@gmx.at> Message-ID: <1582920653.6036306.1552659772699@mail.yahoo.com> Stainers and coverslippers:Let me interject in the discussion:1- Sakura is "hands down" my preferred option because of reliability and results consistency2- Having to have a water free last ethanol is something of the past (besides just proper protocol) on the other hand for years I have dewaxed my sections with dishwasher soap and obtained excellent dewaxing without xylene or ethanol, at all.3- After staining (or any "staining" protocol) I dehydrate any, and I mean any, "stained" by placing them in a 60?C oven during 5-6 minute to completely dry and then to the coverslipper, eliminating, again, ethanol and xylene. Both these two steps (and those dealing with tissue processing) have made my laboratory (and several others) absolutely xylene (or xylene substitutes) free.4- I have always preferred film over glass for speed and simplicity. No problems with "coverslip peeling" if the amount of delivered xylene?(the only instance when I use xylene) is correct.5- Finally, never ask for input to a "vendor" for they all have "tainted" opinions at different levels and will systematically recommend the products they sell. Do your homewotk and ask users, not sllers.I also hope this will helpRen? On Thursday, March 14, 2019 3:59 PM, Gudrun Lang via Histonet wrote: Hi Terri, Have you actually used the Spectra from Leica? This is the follower of ST5020 and CV5020. I am also interested in some feedback on this instrument. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: Terri Braud via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Donnerstag, 14. M?rz 2019 20:10 An: 'histonet at lists.utsouthwestern.edu' Betreff: [Histonet] Stainer vs. Stainer Hi Alison - I've used both stainers and like both of them a lot.? Both were super reliable and easy to use.? However, coverslipping is a different story. I've used both film and glass.? About film - super quick, super easy, but - the purity of the xylene used to coverslip from film must be absolute. Anyone who has experienced film pulling off the slides in storage had a miniscule portion of water carried down the acohols and into the xylene. If it were glass, the process is a bit more forgiving of water contaminent. The absolute alcohols leading to the end xylenes must be kept very fresh.? I kept film slides for over 20 years, no problem.? If you are looking into digital pathology, I would check with vendors to see if film is acceptable. I don't know. As to coverslippers, we've been using the Sakura glass now for 10 years and love it.? I can't compare it to the newer Leica Glass, but 10 years ago my techs all preferred the Sakura because it had fewer moving parts and the maintenance was easier.? I hope this helps.? Good Luck, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal ? 6. stainer v. stainer (Perl , Alison) ? Message: 6 Date: Wed, 13 Mar 2019 20:08:14 +0000 From: "Perl , Alison" To: "'histonet at lists.utsouthwestern.edu'" ??? Subject: [Histonet] stainer v. stainer Hi all We are getting ready to purchase a new H&E stainer/coverslipper, and are considering the Sakura Prisma Plus (tape) and the Leica Spectra (glass). Does anyone have good or bad feedback on either instrument, and/or tape v. glass? We've always had glass, but of course the coverslippers need more maintenance, take longer to dry, more expensive than tape, etc etc. So we are very interested in tape, but still a little hesitant about the old problems of yellowing and peeling after 10+ years. Since we're in NY, we have to keep all slides for 20 years.... Any thoughts are appreciated! Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical 110 South Bedford Rd Mount Kisco, NY 10549 (914) 302-8424 aperl at cmmedical.com www.caremountmedical.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tracy.Pheneger at Colorado.EDU Fri Mar 15 14:44:12 2019 From: Tracy.Pheneger at Colorado.EDU (Tracy Pheneger) Date: Fri, 15 Mar 2019 19:44:12 +0000 Subject: [Histonet] TintoRetriever pressure cooker vs BioCare decloaking chamber? In-Reply-To: References: Message-ID: Hello all; I?m looking to purchase a new pressure cooker for IHC. Anyone have any strong feelings on the TintoRetriever pressure cooker? I have only ever used the Decloaker, but the current price seems outrageous. The TintoRetriever is 1/4th the cost. Thanks, Tracy Tracy Pheneger, HT/HTL (ASCP), QIHC (ASCP), LATg (AALAS) Senior Professional Research Assistant BioServe Space Technologies University of Colorado Engineering Center ECAE 1B02 Boulder, CO 80309-0429 From relia1 at earthlink.net Mon Mar 18 10:43:05 2019 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 18 Mar 2019 11:43:05 -0400 Subject: [Histonet] I have a couple of great cytology opportunities in the Southeast if you know anybody. Message-ID: <04f101d4dda1$47493440$d5db9cc0$@earthlink.net> Hi Histonetters! I hope this is the start of a great week for you! I am hoping someone might be able to help me. I have a couple of really great cytology opportunities in the Southeast, if you know of any cytotechs (including recent grads) that are looking please let me know. Remember if I place someone you refer to me you will earn a referral fee. Have a great day!! Thanks-Pam #jobs4myhistopeeps Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From DavidKemlerLLC at msn.com Mon Mar 18 11:35:18 2019 From: DavidKemlerLLC at msn.com (David) Date: Mon, 18 Mar 2019 16:35:18 +0000 Subject: [Histonet] FS Permanent Mounting Media Message-ID: Histonetters - Our lab is planning on having a FS section slides sent out to a pathologist out-of-state to read. Reading time is not an issue. What mounting media are most folks using to permanently mount the cover glass? Thanks in advance for your help. Yours, Dave From Richard.Cartun at hhchealth.org Mon Mar 18 13:56:18 2019 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 18 Mar 2019 18:56:18 +0000 Subject: [Histonet] ER/PR controls In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EAC11E4C6@HHCEXCHMB03.hhcsystem.org> We use internal control blocks that we prepare from extra tumor tissue that we process after the case has been signed out. However, this is getting more and more difficult for us since most breast tumors are smaller now when detected (and that's a good thing!) which results in the entire tumor being submitted for diagnosis. I like to select a control case where we have multiple blocks (>10), and where the tumor shows variable (weak, moderate, and strong) immunoreactivity for both ER and PR. It's also beneficial when your control tissue contains benign breast epithelium which can serve as both a positive and negative control. In my experience, most breast biopsy specimens show internal positive controls for ER and PR which is especially helpful when the tumor shows a "Triple-Negative" immunoprofile. After all, the best positive control is an internal positive control! When I look at my positive controls on a daily basis, I am looking for reproducibility in the immunoreactivity in the tumor and the benign epithelium. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org -----Original Message----- From: Moe, Barbi A via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 13, 2019 1:44 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] ER/PR controls EXTERNAL email from Outside HHC! Do NOT open attachments or click links from unknown senders. For anyone making in-house ER/PR controls could you comment on what tissue you have in your control block -- one piece of positive tumor regardless of reactivity strength 1+, 2+, 3+ (weak, medium, strong) or multiple pieces to represent each level of reactivity? Also, does anyone order commercially their ER/PR controls to achieve this level of representation in their control block? Any thoughts are appreciated - thank you. Barb Moe Gundersen Health System La Crosse WI bamoe at gundersenhealth.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=Y9uEoty_f-4ICuNypilMcFFogkMgeRwkinFP21id2IE&s=ZE2AOf2Q664rWxQL1OQKmR6u6WuZ8xEv0orVhTW8NJw&e= Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From relia1 at earthlink.net Tue Mar 19 11:14:55 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 19 Mar 2019 12:14:55 -0400 Subject: [Histonet] March Madness is here and it's not just about Basketball!! Message-ID: <000001d4de6e$e43c1a60$acb44f20$@earthlink.net> Hi Histonetters! I Hope You Had a Fun and Lucky St. Patrick?s Day. And A Happy Histotechnology Professionals Day. (Did your lab do anything special?) March Madness has started and in addition to some great College Basketball. I have been experiencing the madness as well. My phone has literally been ringing off the hook with some great new opportunities. All of these positions are permanent full time positions and my clients offer excellent compensation, benefits and relocation assistance. Best of all these clients are motivated to hire and Eager to Meet YOU! Here is a list of my current openings: HISTOLOGY/PATHOLOGY MANAGEMENT GA-Atlanta MA-Cape Cod OH-Columbus (100% travel) WI-Milwaukee HISTOTECHS AK ? Soldotna Yes -Alaska!!! AL ? Birmingham NC - Charlotte CA - Modesto MD ?Baltimore VA ? Norfolk TN ? Chattanooga (CLIA qualified to gross) GA ? Atlanta (CLIA qualified to gross) If you happen to know anyone I need cytotechs in TN and NC!! Histonetters, if you or anyone you know might be interested in the details of any of these positions or want help with a job search in another area please contact me. I can be reached at: cell/text (407)353-5070 or at the office at 866-607-3542 or shoot me an email at relia1 at earthlink.net Remember, If I place someone you refer you will earn a referral $$ reward!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From criley at dpspa.com Tue Mar 19 14:17:30 2019 From: criley at dpspa.com (Charles Riley) Date: Tue, 19 Mar 2019 15:17:30 -0400 Subject: [Histonet] PAP stains Message-ID: What causes dark nuclei in the PAP stain. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From jwwalker at rrmc.org Tue Mar 19 14:50:51 2019 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Tue, 19 Mar 2019 19:50:51 +0000 Subject: [Histonet] PAP stains In-Reply-To: References: Message-ID: There are many reasons why the nuclei may look dark in the Pap stain. 1. What type of hematoxylin are you using? 2. What type of Pap stain are you using, regressive or progressive? This could be due to a variety of things like: type of preparation utilized, too long in hematoxylin, not enough rinsing of excess hematoxylin, not enough time in HCL (if used), too long in bluing, etc., personal preference of interpreting cytotech or pathologist, just to name a few. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Charles Riley via Histonet Sent: Tuesday, March 19, 2019 3:18 PM To: Histo List Subject: [Histonet] PAP stains [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. What causes dark nuclei in the PAP stain. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7Cf0aa0f07b6854504f31408d6ac9fac74%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C636886199125961814&sdata=Attb%2Fexy1igPSs6MNN3nWs8AwHgRBSjYKHOKpr7MXzM%3D&reserved=0 [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] From lblazek at digestivespecialists.com Tue Mar 19 15:23:27 2019 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Tue, 19 Mar 2019 20:23:27 +0000 Subject: [Histonet] job opening Message-ID: We have a Histotech position open in a GI lab located outside Dayton Ohio. This position is Monday - Friday day shift position. We are a growing company that has just opened a brand new facility, with windows so you can actually see outside! You must be HT (ASCP) certified or eligible and have a minimum of one year of experience as a Histology Technician. We work hard to encourage team values, open honest communication and a system of continuous quality improvement. For consideration for this position, please send a cover letter and your resume directly to me at Lblazek at digestivespecialists.com. Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com From jkiernan at uwo.ca Tue Mar 19 23:05:18 2019 From: jkiernan at uwo.ca (John Kiernan) Date: Wed, 20 Mar 2019 04:05:18 +0000 Subject: [Histonet] PAP stains In-Reply-To: References: Message-ID: Charles, What do you mean by "dark nuclei"? Are you asking about the normal colour for the method, or about something you have not seen before that looks wrong? Also, what is "PAP stain"? If "PAP stain" means Papanicoloau, the nuclear stain is Mayer's haemalum. This is a progressive stain; you may need to shorten the time. If "PAP stain" means peroxidase-antiperoxidase (unlabelled antibody-enzyme complex for amplifying detection of HRP-tagged secondary antibodies in immunohistochemistry), nuclei will be dark (usually brown) if they contain the antigen sought by the primary antibody. Nuclear staining might also be a false-positive artifact; if so, it would be present in the simplest of the routine controls (omission of primary antibody). Dark nuclei might also be due to a nuclear counterstain that is too strong. The counterstain should be done in a contrasting colour. Usually it is a progressive haemalum (blue) to contrast with the oxidation product of DAB (brown). Is this a Papanicoloau question or an immunohistochemistry question? Does "PAP stain" have a third meaning? John Kiernan = = = ________________________________ From: Charles Riley via Histonet Sent: 19 March 2019 14:17 To: Histo List Subject: [Histonet] PAP stains What causes dark nuclei in the PAP stain. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang at gmx.at Wed Mar 20 12:10:39 2019 From: gu.lang at gmx.at (Gudrun Lang) Date: Wed, 20 Mar 2019 18:10:39 +0100 Subject: [Histonet] FISH on BondMax Message-ID: <001d01d4df3f$d84ebec0$88ec3c40$@gmx.at> Dear all! I would appreciate input on the FISH-function of the BondMax from Leica. Is this feature reliable and better/as good as manual staining? Would anyone recommend this instrument for FISH staining? Thank you Lang Gudrun From mjdessoye at commonwealthhealth.net Wed Mar 20 14:42:00 2019 From: mjdessoye at commonwealthhealth.net (Dessoye, Michael) Date: Wed, 20 Mar 2019 19:42:00 +0000 Subject: [Histonet] ER/PR/Her2 Controls Message-ID: Hello Histonet, Can anyone recommend a commercial source of ER/PR/Her2 control tissue (array blocks would be preferred)? I'm looking for something similar to the breast analyte control from Cell Marque, which is RUO: http://www.cellmarque.com/cms/histocyte/breast-DR.php Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | Commonwealth Health Laboratory Services | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 ********************************************************************** Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From john.garratt at ciqc.ca Wed Mar 20 15:50:02 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Wed, 20 Mar 2019 20:50:02 +0000 Subject: [Histonet] ER/PR/Her2 Controls In-Reply-To: References: Message-ID: I suggest you contact Mark Rees at StatLab. MRees at statlab.com John www.ciqc.ca ??????? Original Message ??????? On Wednesday, March 20, 2019 12:42 PM, Dessoye, Michael via Histonet wrote: > Hello Histonet, > > Can anyone recommend a commercial source of ER/PR/Her2 control tissue (array blocks would be preferred)? I'm looking for something similar to the breast analyte control from Cell Marque, which is RUO: > > http://www.cellmarque.com/cms/histocyte/breast-DR.php > > Thanks, > Mike > > Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | Commonwealth Health Laboratory Services | mjdessoye at commonwealthhealth.netmailto:mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 > > Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Thu Mar 21 10:14:50 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 21 Mar 2019 15:14:50 +0000 Subject: [Histonet] ER/PR/Her2 Controls In-Reply-To: References: Message-ID: Pantomics has several expression controls and has high quality products. https://www.pantomics.com/tissue-arrays/breast Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: John Garratt via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 20, 2019 1:50 PM To: Dessoye, Michael Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] ER/PR/Her2 Controls I suggest you contact Mark Rees at StatLab. MRees at statlab.com John https://protect2.fireeye.com/url?k=6fdad446-339ab910-6fdaf35b-0cc47adb57f0-5caa0b3d866cb5e0&u=http://www.ciqc.ca/ ??????? Original Message ??????? On Wednesday, March 20, 2019 12:42 PM, Dessoye, Michael via Histonet wrote: > Hello Histonet, > > Can anyone recommend a commercial source of ER/PR/Her2 control tissue (array blocks would be preferred)? I'm looking for something similar to the breast analyte control from Cell Marque, which is RUO: > > https://protect2.fireeye.com/url?k=4d82c7f4-11c2aaa2-4d82e0e9-0cc47adb57f0-946655d0b2e511d4&u=http://www.cellmarque.com/cms/histocyte/breast-DR.php > > Thanks, > Mike > > Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | Commonwealth Health Laboratory Services | mjdessoye at commonwealthhealth.netmailto:mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 > > Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sandra.cheasty at wisc.edu Thu Mar 21 09:44:30 2019 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Thu, 21 Mar 2019 14:44:30 +0000 Subject: [Histonet] Softening Calf Hooves Message-ID: Hello all, I have 1 inch thick cross sections (band saw) of calf hooves. The pathologist has already decaled the bone, and now we have to soften the hooves so she can take thinner sections for processing. * Which is better, potassium hydroxide or sodium hydroxide, and what concentration? * How often should they be checked for softening? * What is the relative air speed of an un-laden swallow? (European) Thanks for your help! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From mward at wakehealth.edu Thu Mar 21 11:59:43 2019 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Thu, 21 Mar 2019 16:59:43 +0000 Subject: [Histonet] Looking for vendor for Zeus Wash solution Message-ID: I am having trouble locating a distributor for the Zeus Wash solution (cat#103) that we use to wash our skin and renal biopsies. Cardinal and Fisher do not seem to carry it and we do not have Zeus Scientific in our system as a vendor we can use. Any help would be appreciated! Thanks, Martha Ward Wake Forest Baptist Health Winston-Salem, NC 27157 From rjbuesa at yahoo.com Thu Mar 21 12:32:26 2019 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 21 Mar 2019 17:32:26 +0000 (UTC) Subject: [Histonet] Softening Calf Hooves In-Reply-To: References: Message-ID: <1596533597.9319247.1553189546783@mail.yahoo.com> Either potassium or sodium hydroxide will work at 10% aq. solution. Check it daily until soft enough.As to your "swallow" question, I "Goggled" your question and the answer is the following" In the end, it's concluded that the airspeed velocity of a (European) unladen swallow is?about 24 miles per hour?or?11 meters per second. But, the real question is not about swallows at all. King Arthur in the movie had two coconut shells that he banged together to simulate the sound of a horse galloping.Jul 7, 2013? Ren? On Thursday, March 21, 2019 12:06 PM, Sandra Cheasty via Histonet wrote: Hello all, ? ? ? ? ? ? ? ? I have 1 inch thick cross sections (band saw) of calf hooves. The pathologist has already decaled the bone, and now we have to soften the hooves so she can take thinner sections for processing. *? ? ? ? Which is better, potassium hydroxide or sodium hydroxide, and what concentration? *? ? ? ? How often should they be checked for softening? *? ? ? ? What is the relative air speed of an un-laden swallow? (European) Thanks for your help! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From paula at excaliburpathology.com Thu Mar 21 12:38:11 2019 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Thu, 21 Mar 2019 17:38:11 +0000 (UTC) Subject: [Histonet] Looking for vendor for Zeus Wash solution In-Reply-To: References: Message-ID: <971057922.8865655.1553189891901@mail.yahoo.com> You can also use Michel's buffer. Electron Microscopy Sciences has it. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953www.excaliburpathology.com On Thursday, March 21, 2019, 12:29:45 PM CDT, Martha Ward-Pathology via Histonet wrote: I am having trouble locating a distributor for the Zeus Wash solution (cat#103) that we use to wash our skin and renal biopsies.? ? Cardinal and Fisher do not seem to carry it and we do not have Zeus Scientific in our system as a vendor we can use.? Any help would be appreciated! Thanks, Martha Ward Wake Forest Baptist Health Winston-Salem, NC 27157 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles at uwhealth.org Thu Mar 21 13:10:48 2019 From: CIngles at uwhealth.org (Ingles Claire) Date: Thu, 21 Mar 2019 18:10:48 +0000 Subject: [Histonet] Softening Calf Hooves In-Reply-To: <1596533597.9319247.1553189546783@mail.yahoo.com> References: , <1596533597.9319247.1553189546783@mail.yahoo.com> Message-ID: Sandra: We here at the Dermatopathology department of UW Mad have found: -10% Potassium Hydroxide works best for us on our nails. -We usually only need to have it in solution for a few hours. As you are trying to soften hoof, I might play with both concentration and time in solution. - I don't believe Monty Python ever actually stated the relative air speed of either type of unladen swallow. ? Say HI to Vicki K for me. Claire Ingles ________________________________ From: Rene J Buesa via Histonet Sent: Thursday, March 21, 2019 12:32 PM To: Sandra Cheasty; Histonet (histonet at lists.utsouthwestern.edu) Subject: Re: [Histonet] Softening Calf Hooves WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Either potassium or sodium hydroxide will work at 10% aq. solution. Check it daily until soft enough.As to your "swallow" question, I "Goggled" your question and the answer is the following" In the end, it's concluded that the airspeed velocity of a (European) unladen swallow is about 24 miles per hour or 11 meters per second. But, the real question is not about swallows at all. King Arthur in the movie had two coconut shells that he banged together to simulate the sound of a horse galloping.Jul 7, 2013 Ren? On Thursday, March 21, 2019 12:06 PM, Sandra Cheasty via Histonet wrote: Hello all, I have 1 inch thick cross sections (band saw) of calf hooves. The pathologist has already decaled the bone, and now we have to soften the hooves so she can take thinner sections for processing. * Which is better, potassium hydroxide or sodium hydroxide, and what concentration? * How often should they be checked for softening? * What is the relative air speed of an un-laden swallow? (European) Thanks for your help! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kelly.Pairan at nationwidechildrens.org Thu Mar 21 14:00:04 2019 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Thu, 21 Mar 2019 19:00:04 +0000 Subject: [Histonet] Microtome Accident Prevention Ideas Message-ID: Good Afternoon Histonetters, Three weeks ago, I was cutting at a manual microtome and when I finished laying my ribbon on the waterbath and turned back to my block, I happened to nick my finger on the end of my microtome blade. It was a freak accident of my finger being in the wrong place at the wrong time. I required 2 stitches. No big deal. Histotechs often have scars. In fact this is my second one on this finger. The last incident was over 10 years ago at another institution. I was invited today to a meeting of some higher level management to discuss the incident and to come up with interventions to prevent these incidents in the future. This was a freak accident that couldn't have been prevented, in my opinion. It is impossible to transfer a ribbon to your waterbath and put the blade guard up at the same time. I do want to come to the meeting with ideas though because I am walking into a room full of people who have never laid eyes on a microtome and I don't want them to come up with ideas for me. Here are some measures that we already have in place: 1) We always lock our wheel when we are not turning it. 2) We put our blade guard up when we are changing out a block or when we walk away from our microtome. 3) After cleaning our microtomes, we put them back together and check to make sure they are locked appropriately. 4) We never leave a blade in the microtome when we are leaving for the day. Thanks for your help, Kelly Kelly Pairan, HT (ASCP)CM, QIHC (ASCP) Histology Supervisor-Anatomic Pathology Department of Pathology and Laboratory Medicine Email: kelly.pairan at nationwidechildrens.org ph: 614-722-5414 fx: 614-722-3033 From kristyn.ferber at gmail.com Fri Mar 22 10:44:47 2019 From: kristyn.ferber at gmail.com (Kristyn Ferber) Date: Fri, 22 Mar 2019 11:44:47 -0400 Subject: [Histonet] Looking for vendor for Zeus Wash solution In-Reply-To: References: Message-ID: You might try searching under Michel Media or Michel's Media. It's the same > but some people refer to it as Zeus and some Michel. > Best, Kristyn > > > ---------- Forwarded message ---------- > From: Martha Ward-Pathology > To: "histonet at lists.utsouthwestern.edu" > > Cc: "histonet at lists2.utsouthwestern.edu" < > histonet at lists2.utsouthwestern.edu> > Bcc: > Date: Thu, 21 Mar 2019 16:59:43 +0000 > Subject: [Histonet] Looking for vendor for Zeus Wash solution > I am having trouble locating a distributor for the Zeus Wash solution > (cat#103) that we use to wash our skin and renal biopsies. Cardinal and > Fisher do not seem to carry it and we do not have Zeus Scientific in our > system as a vendor we can use. Any help would be appreciated! > > Thanks, > > Martha Ward > Wake Forest Baptist Health > Winston-Salem, NC 27157 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Fri Mar 22 10:50:16 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 22 Mar 2019 15:50:16 +0000 Subject: [Histonet] Looking for vendor for Zeus Wash solution In-Reply-To: References: Message-ID: This from Zeus Scientific: They will now handle all IFA reagents from their facility. https://www.zeusscientific.com/news-events/zeus-scientific-to-offer-its-zeus-ifa-product-line-directly-to-customers-in-the-united-states Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Kristyn Ferber via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 22, 2019 8:45 AM To: histonet at lists.utsouthwestern.edu; mward at wakehealth.edu Subject: Re: [Histonet] Looking for vendor for Zeus Wash solution You might try searching under Michel Media or Michel's Media. It's the same > but some people refer to it as Zeus and some Michel. > Best, Kristyn > > > ---------- Forwarded message ---------- > From: Martha Ward-Pathology > To: "histonet at lists.utsouthwestern.edu" > > Cc: "histonet at lists2.utsouthwestern.edu" < > histonet at lists2.utsouthwestern.edu> > Bcc: > Date: Thu, 21 Mar 2019 16:59:43 +0000 > Subject: [Histonet] Looking for vendor for Zeus Wash solution > I am having trouble locating a distributor for the Zeus Wash solution > (cat#103) that we use to wash our skin and renal biopsies. Cardinal and > Fisher do not seem to carry it and we do not have Zeus Scientific in our > system as a vendor we can use. Any help would be appreciated! > > Thanks, > > Martha Ward > Wake Forest Baptist Health > Winston-Salem, NC 27157 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amyleehisto779 at gmail.com Fri Mar 22 15:41:40 2019 From: amyleehisto779 at gmail.com (Amy Lee) Date: Fri, 22 Mar 2019 13:41:40 -0700 Subject: [Histonet] Collagen Hybridizing Peptide Message-ID: Hello histonet, Does anyone ever do this on FFPE tissue? How you feel about this technique and what I should pay attention to? Any commend appreciated. Thanks, Amy From gayle.callis at bresnan.net Mon Mar 25 14:52:12 2019 From: gayle.callis at bresnan.net (Gayle Callis) Date: Mon, 25 Mar 2019 13:52:12 -0600 Subject: [Histonet] Zeus (Michels) fixative and buffer wash Message-ID: <000201d4e344$3e917b70$bbb47250$@bresnan.net> We purchased from Poly Scientific Corp, both the transport media (fixative) and the buffer wash. It comes in several sizes. Another supplier is Newcomer Supply. Gayle Callis GCallis Histology Service LLC Bozeman MT From relia1 at earthlink.net Tue Mar 26 10:58:05 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 26 Mar 2019 11:58:05 -0400 Subject: [Histonet] RELIA Spotlight and Super HOT Histology Opportunities for you and your fellow histopeeps!! Message-ID: <008c01d4e3ec$b341fbe0$19c5f3a0$@earthlink.net> Hello Histonetters, I hope you are having a great week. I wanted to send a quick note to tell you about the positions that I am working on and am most excited about.? Why am I excited about these positions? Because each of these clients was asked if I had someone for you today would you be ready to interview and hire Every One of these clients said YES!!! Here are the Super Sizzlin Hot Spotlight Opportunities! Virginia! Senior Histotechnologist DAYS!!! My client offers an exciting opportunity for a senior histotech. This is a full time permanent dayshift position. You will be responsible routine histology, grossing and IHC. My client has been named one of the top employers in the U.S. and offers an excellent pay rate and benefits along with relocation assistance and a Sign ON Bonus!!! Alaska!! Grossing Histotech ? Days!!! What an adventure!!! My client is looking for a histology tech that can perform routine histology and grossing. This is a full time permanent day shift position in a well-established private lab. Have you ever wanted to explore Alaska? Now is your chance!! Histonetters, I also have some amazing opportunities in these areas!! Charlotte, NC Histotech -nights Birmingham, AL Histotech days Indianapolis, IN Histotech DAYS! Annapolis, MD Histotech DAYS! Modesto, CA IHC Specialist Modesto, CA Histotech All of these positions are full time and permanent!! My clients offer excellent compensation, benefits and in most cases either relocation or a sign-on bonus. If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I Place Someone You Refer You Will Earn A Referral Fee. If you are interested in any of these opportunities CALL /TEXT MY CELL ASAP!!! At 407-353-5070. I can also be reached toll free at the office at 866-607-3542 or at relia1 at earthlink.net to set up a time to talk at your convenience! Thanks-Pam #jobs4myhistopeeps Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From yesyes at comcast.net Tue Mar 26 18:08:59 2019 From: yesyes at comcast.net (MARY ANN) Date: Tue, 26 Mar 2019 23:08:59 -0000 Subject: [Histonet] Fridge temp Message-ID: <255434099.5.1553641737879@localhost> Let's say, hypotheticaly, if you discover your fridge with all you antibodies and detection kits were discovered to have been at 19c. for an undisclosed amout of time. 12 24 48 hours due tona power surge..south Florida weather. Let's also propose your lab CFO/Owner dosent think its a big deal. First how would you handle the issue given the frisge has been restored ? Sent from Xfinity Connect Application From wdesalvo.cac at outlook.com Tue Mar 26 19:19:33 2019 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Wed, 27 Mar 2019 00:19:33 +0000 Subject: [Histonet] Fridge temp In-Reply-To: <255434099.5.1553641737879@localhost> References: <255434099.5.1553641737879@localhost> Message-ID: Simple, revalidate or pitch William DeSalvo ________________________________ From: MARY ANN via Histonet Sent: Tuesday, March 26, 2019 4:09:01 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fridge temp Let's say, hypotheticaly, if you discover your fridge with all you antibodies and detection kits were discovered to have been at 19c. for an undisclosed amout of time. 12 24 48 hours due tona power surge..south Florida weather. Let's also propose your lab CFO/Owner dosent think its a big deal. First how would you handle the issue given the frisge has been restored ? Sent from Xfinity Connect Application _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From john.garratt at ciqc.ca Tue Mar 26 19:23:52 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Wed, 27 Mar 2019 00:23:52 +0000 Subject: [Histonet] Fridge temp In-Reply-To: <255434099.5.1553641737879@localhost> References: <255434099.5.1553641737879@localhost> Message-ID: <3j8kZLiuD4HULDZgKYHCnLXLgLVgbDLyVF_nEcOvvQJUYa1ygM8YZrVwi_ylynZQbFZqg4rVD8o82HoNV1xqxzxEnv3wke0spcPF31K2I4w=@ciqc.ca> Given the cost and importance of IHC reagents the fridge should have a remote alarm on it. It should also be a lab grade fridge and NOT a domestic fridge which will have an auto defrost system to ruin your antibodies ?automatically?. I certainly would not trust a diagnosis or a drug therapy if there?s been improper antibody storage. At a minimum use a data logger to monitor the fridge temperature . They cost less than $100. John Www.ciqc.ca Sent from ProtonMail Mobile On Tue, Mar 26, 2019 at 4:09 PM, MARY ANN via Histonet wrote: > Let's say, hypotheticaly, if you discover your fridge with all you antibodies and detection kits were discovered to have been at 19c. for an undisclosed amout of time. 12 24 48 hours due tona power surge..south Florida weather. > > Let's also propose your lab CFO/Owner dosent think its a big deal. > > First how would you handle the issue given the frisge has been restored ? > > Sent from Xfinity Connect Application > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Tue Mar 26 22:53:04 2019 From: patpxs at gmail.com (P Sicurello) Date: Tue, 26 Mar 2019 20:53:04 -0700 Subject: [Histonet] Fridge temp In-Reply-To: <255434099.5.1553641737879@localhost> References: <255434099.5.1553641737879@localhost> Message-ID: Mary Ann, First I would call the vendor of the antibodies and detection kits and ask them if they have any data about their reagents being warmed up for those time periods. If they can't provide an answer, then I suggest testing every single one of the antibodies and detection kits to see if they still work. I would do something like a mini-validation. Another thing you can do is a literature search that demonstrates the efficacy of warmed up, or old, antibodies/detection kits. Tally up the cost of replacing all the warmed up reagents. Then calculate the cost of running a test that fails due to the warmed reagents - tech time (remember to include whatever percentage the company adds in terms of benefits), reagents used, etc. Also include the effect on the impression of the clients if you need to repeat a test because the first one failed and you were aware of the reason why it failed. Overall, you are better off replacing all of the items that were warmed up. As for the refrigerator, install a temperature tracking system that will notify someone if the temperature goes out of range. If your lab is licensed by any governing agency, let the CFO/Owner know about any rules or regulations that you must follow when it is suspected that a reagent has gone bad, or expires. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Tue, Mar 26, 2019 at 4:15 PM MARY ANN via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Let's say, hypotheticaly, if you discover your fridge with all you > antibodies and detection kits were discovered to have been at 19c. for an > undisclosed amout of time. 12 24 48 hours due tona power surge..south > Florida weather. > > Let's also propose your lab CFO/Owner dosent think its a big deal. > > First how would you handle the issue given the frisge has been restored ? > > > > > Sent from Xfinity Connect Application > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bakevictoria at gmail.com Wed Mar 27 08:04:27 2019 From: bakevictoria at gmail.com (Victoria Baker) Date: Wed, 27 Mar 2019 09:04:27 -0400 Subject: [Histonet] ISH kappa lambda on bone marrow core biopsy Message-ID: Good morning! Just recently we started having an issue with a heavy blue precipitate on our bone marrow cores. We have changed nothing in our protocol and even did parallel testing with a reference lab. Both had the precipitate, but to a lesser intensity. Has anyone else been seeing this? Thank you in advance any and responses are appreciated. Vikki From Timothy.Morken at ucsf.edu Wed Mar 27 10:29:54 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 27 Mar 2019 15:29:54 +0000 Subject: [Histonet] FW: Fridge temp References: <255434099.5.1553641737879@localhost> Message-ID: Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Morken, Timothy Sent: Wednesday, March 27, 2019 8:30 AM To: 'P Sicurello'; MARY ANN Subject: RE: [Histonet] Fridge temp You will need to retest to confirm reliability. That would be easier that buying all new -which you would then have to test anyway. In the old, old days most antibodies were serum-based and were susceptible to degradation and bacterial growth. Modern antibodies and buffer formulations are very robust and I think you will find that few, if any, will fail. When I worked for an antibody company I used to do accelerated longevity testing by putting antibodies in a 40C oven for several MONTHS. I can't recall any failing, and some worked even better after that exposure! However, some that have been opened and could show bacterial contamination. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, March 26, 2019 8:53 PM To: MARY ANN Cc: HistoNet Subject: Re: [Histonet] Fridge temp Mary Ann, First I would call the vendor of the antibodies and detection kits and ask them if they have any data about their reagents being warmed up for those time periods. If they can't provide an answer, then I suggest testing every single one of the antibodies and detection kits to see if they still work. I would do something like a mini-validation. Another thing you can do is a literature search that demonstrates the efficacy of warmed up, or old, antibodies/detection kits. Tally up the cost of replacing all the warmed up reagents. Then calculate the cost of running a test that fails due to the warmed reagents - tech time (remember to include whatever percentage the company adds in terms of benefits), reagents used, etc. Also include the effect on the impression of the clients if you need to repeat a test because the first one failed and you were aware of the reason why it failed. Overall, you are better off replacing all of the items that were warmed up. As for the refrigerator, install a temperature tracking system that will notify someone if the temperature goes out of range. If your lab is licensed by any governing agency, let the CFO/Owner know about any rules or regulations that you must follow when it is suspected that a reagent has gone bad, or expires. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Tue, Mar 26, 2019 at 4:15 PM MARY ANN via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Let's say, hypotheticaly, if you discover your fridge with all you > antibodies and detection kits were discovered to have been at 19c. for an > undisclosed amout of time. 12 24 48 hours due tona power surge..south > Florida weather. > > Let's also propose your lab CFO/Owner dosent think its a big deal. > > First how would you handle the issue given the frisge has been restored ? > > > > > Sent from Xfinity Connect Application > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Monica.Lockhart at luhs.org Wed Mar 27 12:10:03 2019 From: Monica.Lockhart at luhs.org (MONICA D. LOCKHART) Date: Wed, 27 Mar 2019 17:10:03 +0000 Subject: [Histonet] Fridge temp In-Reply-To: References: <255434099.5.1553641737879@localhost> Message-ID: <2917ae084c1a440c9fc034db89e7b3a6@luhs.org> I agree !! The integrity of the antibody is suspect after being exposed to temps like that. Even if the fridge door was kept closed during the outtage. Monica D. Lockhart -----Original Message----- From: WILLIAM DESALVO [mailto:wdesalvo.cac at outlook.com] Sent: Tuesday, March 26, 2019 7:20 PM To: MARY ANN; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Fridge temp Simple, revalidate or pitch William DeSalvo ________________________________ From: MARY ANN via Histonet Sent: Tuesday, March 26, 2019 4:09:01 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fridge temp Let's say, hypotheticaly, if you discover your fridge with all you antibodies and detection kits were discovered to have been at 19c. for an undisclosed amout of time. 12 24 48 hours due tona power surge..south Florida weather. Let's also propose your lab CFO/Owner dosent think its a big deal. First how would you handle the issue given the frisge has been restored ? Sent from Xfinity Connect Application _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From criley at dpspa.com Wed Mar 27 12:35:43 2019 From: criley at dpspa.com (Charles Riley) Date: Wed, 27 Mar 2019 13:35:43 -0400 Subject: [Histonet] Fridge temp In-Reply-To: <2917ae084c1a440c9fc034db89e7b3a6@luhs.org> References: <255434099.5.1553641737879@localhost> <2917ae084c1a440c9fc034db89e7b3a6@luhs.org> Message-ID: Some facilities run their immunostainers on a continuous basis all week long and antibodies may never leave the machine for multiple days. The antibody is still valid and still works. Like a previous responder said you can test each one and compare to previously stained slides to validate it is still working. I don't remember where I heard it but someone published that for each hour an antibody is not keep within it's storage temperature range you lose 1 day of effective usage. (so if the antibodies were out of temp for 24 hours you lose 24 days from the printed expiration date) On Wed, Mar 27, 2019 at 1:24 PM MONICA D. LOCKHART via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I agree !! The integrity of the antibody is suspect after being exposed to > temps like that. Even if the fridge door was kept closed during the > outtage. > > Monica D. Lockhart > > > -----Original Message----- > From: WILLIAM DESALVO [mailto:wdesalvo.cac at outlook.com] > Sent: Tuesday, March 26, 2019 7:20 PM > To: MARY ANN; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Fridge temp > > Simple, revalidate or pitch > > William DeSalvo > ________________________________ > From: MARY ANN via Histonet > Sent: Tuesday, March 26, 2019 4:09:01 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Fridge temp > > Let's say, hypotheticaly, if you discover your fridge with all you > antibodies and detection kits were discovered to have been at 19c. for an > undisclosed amout of time. 12 24 48 hours due tona power surge..south > Florida weather. > > Let's also propose your lab CFO/Owner dosent think its a big deal. > > First how would you handle the issue given the frisge has been restored ? > > > > > Sent from Xfinity Connect Application > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From greg.dobbin at gmail.com Wed Mar 27 13:13:32 2019 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Wed, 27 Mar 2019 15:13:32 -0300 Subject: [Histonet] Histonet Digest, Vol 184, Issue 23 In-Reply-To: References: Message-ID: Hello all, While I do not disagree with anything that has been said thus far regarding the failed refrigerator and moving forward, I think Tim Morken's information and shared experience was the most relevant! I know that my Bond instruments are running 24 hrs a day, so some of the more commonly ordered antibodies and certainly our detection kits (enzyme included) can be out of the fridge for days with no noticeable deterioration. :-) Greg > > ---------- Forwarded message ---------- > From: MARY ANN > To: > Cc: > Bcc: > Date: > Subject: [Histonet] Fridge temp > Let's say, hypotheticaly, if you discover your fridge with all you > antibodies and detection kits were discovered to have been at 19c. for an > undisclosed amout of time. 12 24 48 hours due tona power surge..south > Florida weather. > > Let's also propose your lab CFO/Owner dosent think its a big deal. > > First how would you handle the issue given the frisge has been restored ? > > > > -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From relia1 at earthlink.net Wed Mar 27 13:17:24 2019 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 27 Mar 2019 14:17:24 -0400 Subject: [Histonet] Histology Supervisor/QA Coordinator needed can you help? Message-ID: <055e01d4e4c9$54065e90$fc131bb0$@earthlink.net> Hi Histonetters, How are you? I hope you are having a great week! I have an exciting opportunity to share and if you aren?t interested maybe you know someone who might be! I have been engaged on an exclusive search by one of my best clients located near Dallas, TX that is in need of a: QA Coordinator-Histology This is a full time permanent position in a suburb just outside of Dallas. My client is looking for an ASCP certified HT/HTL with strong routine histology and IHC. This person will be responsible for: ? Assisting with on boarding of new hires ? Training of staff ? Identifies, investigates and documents all non-conforming and quality events ? Participates in IHC validation process ? Equipment maintenance and communication to staff ? Coordinates with QA committee to conduct monthly staff meetings ? Presents archived CAP and safety training sessions to staff ? Serves as routine histology and IHC technician capacity as needed They are offering an excellent compensation package. The help I need from you Histonetters is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them or pass their contact information to me? *remember if I place someone you refer to me you will earn a referral bonus! If you are interested in this position please contact me ASAP on my cell/text 407-353-5070 or toll free at 866-607-3542 or via email at relia1 at earthlink.net If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won?t release it to anyone without your permission. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From madeathridge at pastnashville.com Fri Mar 29 10:47:52 2019 From: madeathridge at pastnashville.com (Maryann Deathridge) Date: Fri, 29 Mar 2019 15:47:52 +0000 Subject: [Histonet] Gemini Stainer Message-ID: HELP, Please--- Anyone know the programming of the Thermo Varistainer Gemini the older model. Questions regarding the water trough if you don't want slides to go to the W4/5/6/. Maryann Deathridge, Lab Manager Pathology Assoc. of St. Thomas 4220 Harding Pike Bldg. SE, Suite 504 Nashville, TN 37205 (615) 298-4100 madeathridge at pastnashville.com From madeathridge at pastnashville.com Fri Mar 29 12:34:54 2019 From: madeathridge at pastnashville.com (Maryann Deathridge) Date: Fri, 29 Mar 2019 17:34:54 +0000 Subject: [Histonet] Gemini Stainer In-Reply-To: References: Message-ID: Thanks for all the responses. I call Thermo as always they were a GREAT help. #easierday -----Original Message----- From: Maryann Deathridge via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 29, 2019 10:48 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Gemini Stainer HELP, Please--- Anyone know the programming of the Thermo Varistainer Gemini the older model. Questions regarding the water trough if you don't want slides to go to the W4/5/6/. Maryann Deathridge, Lab Manager Pathology Assoc. of St. Thomas 4220 Harding Pike Bldg. SE, Suite 504 Nashville, TN 37205 (615) 298-4100 madeathridge at pastnashville.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Caroline.Pratt at uphs.upenn.edu Fri Mar 29 13:25:28 2019 From: Caroline.Pratt at uphs.upenn.edu (Pratt, Caroline) Date: Fri, 29 Mar 2019 18:25:28 +0000 Subject: [Histonet] Salt Split DIF Message-ID: <14D6A469D20B4F4AACC47C3E973C3FA80178DC0E@UPHMASPHI030.UPHS.PENNHEALTH.PRV> Has anyone every had a very difficult time getting the skin to split even after soaking for days in 2N saline? Caroline M. Pratt, MBA Practice Administrator Dermpath 3020 Market Street, Ste 201 Philadelphia, PA 19104 Phone 215-349-8178 Cell 610-800-1381 Fax 215-662-6150