[Histonet] FISH for breast core (Her2)

[Mark Tarango]

 Hello Taganrog,

If you are having issues with high autoflorescence due to prolonged
formalin fixation (7 days), it might help to do a few minutes extra
protease digestion (5+ minutes) during tissue pretreatment.  Alternately or
in conjunction, I would suggest re-applying HER2 probe to the same slide
that had already been pretreated and hybridized.  By doing this a few times
(even up to four tries), you will likely get FISH signals that you can see
and enumerate.

Good luck,

Mark Tarango

On Tue, Jun 11, 2019 at 11:33 AM Пешков Максим via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

>
> Dear colleagues!
> Can anyone help? We are very appreciatated any suggestions, tips and hacks
> for Her2 FISH onto breast trepine fixed in 10% NBF. It was here for more
> than 7 days, then processed as usual into wax. IHC for Her2 has value as 2+.
> Sincerely,
> Maxim Peshkov,
> Russia,
> Taganrog.
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