From garethdavisyuma at gmail.com Mon Jul 1 16:51:37 2019 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Mon, 1 Jul 2019 14:51:37 -0700 Subject: [Histonet] Laboratory Equipment services and supplies Message-ID: Has anyone used Laboratory Equipment Services and supplies, LLC for their preventative maintenance? Just wondering if they are good. They sent me a quote that was really good and much less than what I have been quoted. Any feed back would be appreciated. Gareth -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From sbeckercatania at revealbio.com Mon Jul 1 18:08:59 2019 From: sbeckercatania at revealbio.com (Sara Becker-Catania) Date: Mon, 1 Jul 2019 16:08:59 -0700 Subject: [Histonet] Job Posting Message-ID: Dear Histonet, We have an opening for a position that would likely be of interest to subscribers on your mailing list (see below). If you could post it, we would appreciate it. Best Regards, Sara Catania Histologist (Full Time) Are you a talented Histologist looking for a challenging role in an enthusiastic and upbeat small company? Do you work to a high standard and enjoy variety? Reveal is a San Diego-based company specializing in advanced tissue technologies and computational pathology. We provide research products and services to a wide range of biopharma clientele throughout the world. We are also applying artificial intelligence to develop novel pathology diagnostics. We are seeking a Histologist with a high degree of proficiency in routine histology to join our growing team. The successful candidate will be responsible for tissue processing, embedding, paraffin sectioning, cryosectioning, H&E staining, special stains, immunohistochemistry (IHC) and digital pathology. Experience with in situ hybridization (ISH), automated IHC (particularly use of Leica Bond instruments) and the use of whole slide scanners is an advantage but not essential. We offer benefits, a flexible work environment, and professional growth opportunities for the right candidate. Requirements: a Bachelor?s degree in biological sciences or a related subject plus at least two years experience in histology. Certification as a Histotechnician (HT) or Histotechnologist (HTL) is desirable. Strong communication skills and a willingness and flexibility to contribute to a dynamic team environment are essential. For confidential consideration please send your resume and salary requirements to careers at revealbio.com *Sara Becker Catania, PhD *Associate Director Product Development 6760 Top Gun St, Ste 110 San Diego, CA 92121 858.274.3663 | RevealBio.com From beth.oneil1 at wvumedicine.org Wed Jul 3 13:19:34 2019 From: beth.oneil1 at wvumedicine.org (O'Neil, Beth) Date: Wed, 3 Jul 2019 18:19:34 +0000 Subject: [Histonet] HSV-2 antibody - Cell Marque was not the problem Message-ID: <3CEB8EBCF9C7A648B9694B5696462A71A665D104@NT-EX1.wvuhs.com> Last week I posted an inquiry about having problems with Cell Marque's HSV-2 polyclonal antibody. I have to retract my statement that it was Cell Marque using a different antibody source. After spending two weeks troubleshooting my stainer, troubleshooting the positive control slides, yelling at my Cell Marque rep, etc. I found out that it was the positive control slide and not the antibody. When I originally suspected the positive QC slides (from Cancer Diagnostics), I requested a different lot number. This new lot also failed to show positive staining. Long story short, another call to Cancer Diagnostics finally resulted in receiving confirmation that they are having problems with their HSV-2 control slides. It was my misfortune to have opened a new box of QC slides at the same time as opening a new vial of HSV-2 antibody which resulted in two weeks of headaches. I will say that Cell Marque/Millipore was very supportive through this. So, for those of you who are using Cancer Diagnostics HSV-2 control slides, they are working on a resolution and will replace their "bad" slides. I was told that the staining is "hit or miss, " hopefully you all have the "hit" slides. Beth Oneil, WVU Medicine Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From saby_joseph_a at yahoo.com Thu Jul 4 07:56:24 2019 From: saby_joseph_a at yahoo.com (Joseph Saby) Date: Thu, 4 Jul 2019 12:56:24 +0000 (UTC) Subject: [Histonet] HSV-2 antibody - Cell Marque was not the problem In-Reply-To: <3CEB8EBCF9C7A648B9694B5696462A71A665D104@NT-EX1.wvuhs.com> References: <3CEB8EBCF9C7A648B9694B5696462A71A665D104@NT-EX1.wvuhs.com> Message-ID: <602033116.2817951.1562244984551@mail.yahoo.com> Beth- I am proud of you for stepping up and correcting the record. This could not be an easy thing to do in such a public forum, but it also essential. I hope you have a wonderful holiday. Joe Saby On Wednesday, July 3, 2019, 2:33:16 PM EDT, O'Neil, Beth via Histonet wrote: Last week I posted an inquiry about having problems with Cell Marque's HSV-2 polyclonal antibody.? I have to retract my statement that it was Cell Marque using a different antibody source.? After spending two weeks troubleshooting my stainer, troubleshooting the positive control slides, yelling at my Cell Marque rep, etc.? I found out that it was the positive control slide and not the antibody.? When I originally suspected the positive QC slides (from Cancer Diagnostics), I requested a different lot number.? This new lot also failed to show positive staining.? Long story short, another call to Cancer Diagnostics finally resulted in receiving confirmation that they are having problems with their HSV-2 control slides.? It was my misfortune to have opened a new box of QC slides at the same time as opening a new vial of HSV-2 antibody which resulted in two weeks of headaches.? I will say that Cell Marque/Millipore was very supportive through this.? So, for those of you who are using Cancer Diagnostics HSV-2 control slides, they are working on a resolution and will replace their "bad" slides.? I was told that the staining is "hit or miss, "? hopefully you all have the "hit" slides. Beth Oneil, WVU Medicine Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kelly.Pairan at nationwidechildrens.org Fri Jul 5 09:41:28 2019 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Fri, 5 Jul 2019 14:41:28 +0000 Subject: [Histonet] Productivity Tracking Message-ID: <4fec896acbc64d2496b231c93934d526@l1perdwmbx01.childrensroot.net> Good Morning Histoland, How are you tracking your histotechs productivity when it comes to cutting? We recently have implemented a 25 block per hour goal for all of our histotechs and are receiving some push back. I made 25 block per hour the goal based on the following article that has been circulating for many years (https://www.researchgate.net/publication/41942535_Productivity_standards_for_histology_laboratories). While I do not want to compromise quality, we do have turnaround times to meet and I have experienced techs who are cutting less than 20 blocks per hour. I understand that some tissues and protocols take longer so this is an average not something that has to be hit every shift. Thanks, Kelly Kelly Pairan, HT (ASCP)CM, QIHC (ASCP) Histology Supervisor-Anatomic Pathology Department of Pathology and Laboratory Medicine Email: kelly.pairan at nationwidechildrens.org ph: 614-722-5414 fx: 614-722-3033 From Christopher.Sheeder at seattlechildrens.org Fri Jul 5 10:55:49 2019 From: Christopher.Sheeder at seattlechildrens.org (Sheeder, Christopher) Date: Fri, 5 Jul 2019 15:55:49 +0000 Subject: [Histonet] HSV-2 antibody - Cell Marque was not the problem In-Reply-To: <3CEB8EBCF9C7A648B9694B5696462A71A665D104@NT-EX1.wvuhs.com> References: <3CEB8EBCF9C7A648B9694B5696462A71A665D104@NT-EX1.wvuhs.com> Message-ID: Beth, You have honor and integrity. Something seldom seen these days. Chris Sheeder Seattle Children's Hospital -----Original Message----- From: O'Neil, Beth Sent: Wednesday, July 03, 2019 11:20 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] HSV-2 antibody - Cell Marque was not the problem Last week I posted an inquiry about having problems with Cell Marque's HSV-2 polyclonal antibody. I have to retract my statement that it was Cell Marque using a different antibody source. After spending two weeks troubleshooting my stainer, troubleshooting the positive control slides, yelling at my Cell Marque rep, etc. I found out that it was the positive control slide and not the antibody. When I originally suspected the positive QC slides (from Cancer Diagnostics), I requested a different lot number. This new lot also failed to show positive staining. Long story short, another call to Cancer Diagnostics finally resulted in receiving confirmation that they are having problems with their HSV-2 control slides. It was my misfortune to have opened a new box of QC slides at the same time as opening a new vial of HSV-2 antibody which resulted in two weeks of headaches. I will say that Cell Marque/Millipore was very supportive through this. So, for those of you who are using Cancer Diagnostics HSV-2 control slides, they are working on a resolution and will replace their "bad" slides. I was told that the staining is "hit or miss, " hopefully you all have the "hit" slides. Beth Oneil, WVU Medicine Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From ajju33 at gmail.com Fri Jul 5 12:31:36 2019 From: ajju33 at gmail.com (Muhammad Azam) Date: Fri, 5 Jul 2019 13:31:36 -0400 Subject: [Histonet] HSV-2 antibody - Cell Marque was not the problem In-Reply-To: References: <3CEB8EBCF9C7A648B9694B5696462A71A665D104@NT-EX1.wvuhs.com> Message-ID: Bravo Beth Sent from my iPhone > On Jul 5, 2019, at 11:55 AM, Sheeder, Christopher via Histonet wrote: > > Beth, > You have honor and integrity. Something seldom seen these days. > > Chris Sheeder > Seattle Children's Hospital > > -----Original Message----- > From: O'Neil, Beth > Sent: Wednesday, July 03, 2019 11:20 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] HSV-2 antibody - Cell Marque was not the problem > > Last week I posted an inquiry about having problems with Cell Marque's HSV-2 polyclonal antibody. I have to retract my statement that it was Cell Marque using a different antibody source. After spending two weeks troubleshooting my stainer, troubleshooting the positive control slides, yelling at my Cell Marque rep, etc. I found out that it was the positive control slide and not the antibody. When I originally suspected the positive QC slides (from Cancer Diagnostics), I requested a different lot number. This new lot also failed to show positive staining. Long story short, another call to Cancer Diagnostics finally resulted in receiving confirmation that they are having problems with their HSV-2 control slides. It was my misfortune to have opened a new box of QC slides at the same time as opening a new vial of HSV-2 antibody which resulted in two weeks of headaches. I will say that Cell Marque/Millipore was very supportive through this. So, for those of you who are using Cancer Diagnostics HSV-2 control slides, they are working on a resolution and will replace their "bad" slides. I was told that the staining is "hit or miss, " hopefully you all have the "hit" slides. > > Beth Oneil, WVU Medicine > > > > > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wdesalvo.cac at outlook.com Fri Jul 5 12:46:03 2019 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Fri, 5 Jul 2019 17:46:03 +0000 Subject: [Histonet] Productivity Tracking In-Reply-To: <4fec896acbc64d2496b231c93934d526@l1perdwmbx01.childrensroot.net> References: <4fec896acbc64d2496b231c93934d526@l1perdwmbx01.childrensroot.net> Message-ID: I suggest you use slides created for the microtomy minimum standard. Not all blocks are created equal. A good target is 30 slides per hour, for mixed specimens. If all specialty, then adjust from the 30. William DeSalvo ________________________________ From: Pairan, Kelly via Histonet Sent: Friday, July 5, 2019 8:41:28 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Productivity Tracking Good Morning Histoland, How are you tracking your histotechs productivity when it comes to cutting? We recently have implemented a 25 block per hour goal for all of our histotechs and are receiving some push back. I made 25 block per hour the goal based on the following article that has been circulating for many years (https://www.researchgate.net/publication/41942535_Productivity_standards_for_histology_laboratories). While I do not want to compromise quality, we do have turnaround times to meet and I have experienced techs who are cutting less than 20 blocks per hour. I understand that some tissues and protocols take longer so this is an average not something that has to be hit every shift. Thanks, Kelly Kelly Pairan, HT (ASCP)CM, QIHC (ASCP) Histology Supervisor-Anatomic Pathology Department of Pathology and Laboratory Medicine Email: kelly.pairan at nationwidechildrens.org ph: 614-722-5414 fx: 614-722-3033 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren at gmail.com Sat Jul 6 13:12:06 2019 From: jaylundgren at gmail.com (Jay Lundgren) Date: Sat, 6 Jul 2019 13:12:06 -0500 Subject: [Histonet] Productivity Tracking In-Reply-To: References: <4fec896acbc64d2496b231c93934d526@l1perdwmbx01.childrensroot.net> Message-ID: By the way, the standard for graduation from AFIP was 30 blocks/hr. Don't count blocks, count *slides*. If you have people slacking, you really need to start action to get rid of them. One rotten apple ruins the whole bunch. Google the new research on toxic personalities in the workplace. But if you want to try to bring them up to speed, try this: (Assuming one processing run, but the principle still applies.) 1)Someone takes the day's worksheet, and counts the number of slides to be cut, including recuts, special stains, IHC's and everything. So, according to your protocol, (numbers made up for example) 1 GI block=3 slides, an IHC panel= 10 slides, a routine tonsil block=1 slide, a bone marrow block, with specials and unstained slides=20 slides 2)Divide the number of slides to be cut by the number of cutters. 3)Distribute the blocks equally based on number of *slides *to be cut per tech. Now obviously, you're going to want to give your biopsies, recuts, specials and IHCs to your quickest cutters, and on down the line, in order of priority, to the slower cutters. If you only have 2 cutters, one person is going to cut all the bxs and specials and a few routines, and the other person will cut the bulk of the routines. This daily routine achieves 3 things. 1) It keeps the slower cutters from slowing down your more critical workflow. 2) It removes any benefit from slacking, because it attempts to ensure that everyone is doing the same amount of work, at least as far as cutting goes. 3) Most importantly, it identifies and isolates anyone who is cutting slower, because they will still be sitting there cutting while everyone else is done. While this might seem cruel, most humans are very group oriented. If the slower cutters are experienced cutter who are sandbagging, they will usually pick up their pace. If someone is inexperienced and trying to get faster, that's OK, and you can focus on trying to help them get faster. A very small minority of people might be happy to sit there and cut their routines as slowly as possible, and you have to decide whether or not to start the process of getting rid of people like that. At least dividing the cutting like this avoids them slowing down your workflow too much. You can carrot and stick this however you want. I see you are a histology supervisor, so I'm assuming it's actually stick/stick. (j/k :)) Maybe not let anyone go to lunch until all the cutting is done? Let the fastest cutters leave 30 min early on a Friday? Send a clear message that it's not OK just to slack, you want everyone working to their potential. On Fri, Jul 5, 2019 at 12:59 PM WILLIAM DESALVO via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I suggest you use slides created for the microtomy minimum standard. Not > all blocks are created equal. A good target is 30 slides per hour, for > mixed specimens. If all specialty, then adjust from the 30. > > William DeSalvo > ________________________________ > From: Pairan, Kelly via Histonet > Sent: Friday, July 5, 2019 8:41:28 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Productivity Tracking > > Good Morning Histoland, > How are you tracking your histotechs productivity when it comes to > cutting? We recently have implemented a 25 block per hour goal for all of > our histotechs and are receiving some push back. I made 25 block per hour > the goal based on the following article that has been circulating for many > years ( > https://www.researchgate.net/publication/41942535_Productivity_standards_for_histology_laboratories). > While I do not want to compromise quality, we do have turnaround times to > meet and I have experienced techs who are cutting less than 20 blocks per > hour. I understand that some tissues and protocols take longer so this is > an average not something that has to be hit every shift. > > Thanks, > Kelly > > Kelly Pairan, HT (ASCP)CM, QIHC (ASCP) > Histology Supervisor-Anatomic Pathology > Department of Pathology and Laboratory Medicine > Email: kelly.pairan at nationwidechildrens.org > ph: 614-722-5414 > fx: 614-722-3033 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jasonhauser71 at gmail.com Sun Jul 7 14:02:24 2019 From: jasonhauser71 at gmail.com (jasonhauser71 at gmail.com) Date: Sun, 07 Jul 2019 15:02:24 -0400 Subject: [Histonet] Productivity Tracking Message-ID: Really? If I am the faster cutter getting saddled with the more critical work, I would expect better pay than the slower cutter relegated to routine work.??If one managesfrom the standpoint of individual productivity- one will never be satisfied. The slowest and least produ tive can be fired or laid off...that just means there is a new victimJason HauserSr. Histology Tecnician, MLTThe South Bend ClinicSouth Bend Indiana------ Original message------From: Jay LundgrenDate: Sat, Jul 6, 2019 2:12 PMTo: WILLIAM DESALVO;Cc: Pairan, Kelly;histonet at lists.utsouthwestern.edu;Subject:Re: [Histonet] Productivity TrackingBy the way, the standard for graduation from AFIP was 30 blocks/hr. Don't count blocks, count *slides*. If you have people slacking, you really need to start action to get rid of them. One rotten apple ruins the whole bunch. Google the new research on toxic personalities in the workplace. But if you want to try to bring them up to speed, try this: (Assuming one processing run, but the principle still applies.) 1)Someone takes the day's worksheet, and counts the number of slides to be cut, including recuts, special stains, IHC's and everything. So, according to your protocol, (numbers made up for example) 1 GI block=3 slides, an IHC panel= 10 slides, a routine tonsil block=1 slide, a bone marrow block, with specials and unstained slides=20 slides 2)Divide the number of slides to be cut by the number of cutters. 3)Distribute the blocks equally based on number of *slides *to be cut per tech. Now obviously, you're going to want to give your biopsies, recuts, specials and IHCs to your quickest cutters, and on down the line, in order of priority, to the slower cutters. If you only have 2 cutters, one person is going to cut all the bxs and specials and a few routines, and the other person will cut the bulk of the routines. This daily routine achieves 3 things. 1) It keeps the slower cutters from slowing down your more critical workflow. 2) It removes any benefit from slacking, because it attempts to ensure that everyone is doing the same amount of work, at least as far as cutting goes. 3) Most importantly, it identifies and isolates anyone who is cutting slower, because they will still be sitting there cutting while everyone else is done. While this might seem cruel, most humans are very group oriented. If the slower cutters are experienced cutter who are sandbagging, they will usually pick up their pace. If someone is inexperienced and trying to get faster, that's OK, and you can focus on trying to help them get faster. A very small minority of people might be happy to sit there and cut their routines as slowly as possible, and you have to decide whether or not to start the process of getting rid of people like that. At least dividing the cutting like this avoids them slowing down your workflow too much. You can carrot and stick this however you want. I see you are a histology supervisor, so I'm assuming it's actually stick/stick. (j/k :)) Maybe not let anyone go to lunch until all the cutting is done? Let the fastest cutters leave 30 min early on a Friday? Send a clear message that it's not OK just to slack, you want everyone working to their potential. On Fri, Jul 5, 2019 at 12:59 PM WILLIAM DESALVO via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I suggest you use slides created for the microtomy minimum standard. Not > all blocks are created equal. A good target is 30 slides per hour, for > mixed specimens. If all specialty, then adjust from the 30. > > William DeSalvo > ________________________________ > From: Pairan, Kelly via Histonet > Sent: Friday, July 5, 2019 8:41:28 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Productivity Tracking > > Good Morning Histoland, > How are you tracking your histotechs productivity when it comes to > cutting? We recently have implemented a 25 block per hour goal for all of > our histotechs and are receiving some push back. I made 25 block per hour > the goal based on the following article that has been circulating for many > years ( > https://www.researchgate.net/publication/41942535_Productivity_standards_for_histology_laboratories). > While I do not want to compromise quality, we do have turnaround times to > meet and I have experienced techs who are cutting less than 20 blocks per > hour. I understand that some tissues and protocols take longer so this is > an average not something that has to be hit every shift. > > Thanks, > Kelly > > Kelly Pairan, HT (ASCP)CM, QIHC (ASCP) > Histology Supervisor-Anatomic Pathology > Department of Pathology and Laboratory Medicine > Email: kelly.pairan at nationwidechildrens.org > ph: 614-722-5414 > fx: 614-722-3033 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jaylundgren at gmail.com Sun Jul 7 15:20:56 2019 From: jaylundgren at gmail.com (Jay Lundgren) Date: Sun, 7 Jul 2019 15:20:56 -0500 Subject: [Histonet] Productivity Tracking In-Reply-To: References: Message-ID: As I pointed out, management can incentivize this any way they want. You could give raises, or you could say a tech is not eligible for a raise until they can cut at a certain rate. The idea is to bring the slower cutters up to speed, not get rid of them. Based on my experience, if you isolate and identify the malingerers, they will most likely speed up of their own accord. Most people will stop their BS once they realize everyone is on to them. The majority of people like to belong to a group, and don't like to be thought of as being bad at their job. Only if they absolutely refuse to try to speed up would you start the process of getting rid of them, and only in certain situations. We're talking the truly toxic employee. At least this way, the slower cutters don't slow down your whole workflow, and it attempts to ensure some fairness in the amount of cutting each tech does. Also, you don't have to burden the faster cutters with more paperwork for productivity reporting, not to mention the hours it will take for management to collate and interpret the data. It just takes someone counting the slides daily, which takes a couple of minutes tops. But your comment is a good example. Eventually, if everyone is not pulling together, the faster techs will start feeling taken advantage of, and change their own attitude towards work as well. Why would you feel that you are "getting saddled" with critical work, rather than feeling proud that management has recognized your talent and is utilizing it efficiently to improve patient care? Do you feel like its more work to cut 60 slides of bxs, specials, and IHCs (say, 10 blocks) than 60 slides of routine surgicals (60 blocks)? Personally, I'd rather cut the bxs. On Sun, Jul 7, 2019 at 2:02 PM jasonhauser71 at gmail.com < jasonhauser71 at gmail.com> wrote: > Really? If I am the faster cutter getting saddled with the more critical > work, I would expect better pay than the slower cutter relegated to routine > work. > If one managesfrom the standpoint of individual productivity- one will > never be satisfied. The slowest and least produ tive can be fired or laid > off...that just means there is a new victim > > > Jason Hauser > Sr. Histology Tecnician, MLT > The South Bend Clinic > South Bend Indiana > > ------ Original message------ > *From: *Jay Lundgren > *Date: *Sat, Jul 6, 2019 2:12 PM > *To: *WILLIAM DESALVO; > *Cc: *Pairan, Kelly;histonet at lists.utsouthwestern.edu; > *Subject:*Re: [Histonet] Productivity Tracking > > By the way, the standard for graduation from AFIP was 30 blocks/hr. > > Don't count blocks, count *slides*. > > If you have people slacking, you really need to start action to get rid of > them. One rotten apple ruins the whole bunch. Google the new research on > toxic personalities in the workplace. > > But if you want to try to bring them up to speed, try this: (Assuming one > processing run, but the principle still applies.) > > 1)Someone takes the day's worksheet, and counts the number of slides to be > cut, including recuts, special stains, IHC's and everything. So, > according to your protocol, (numbers made up for example) 1 GI block=3 > slides, an IHC panel= 10 slides, a routine tonsil block=1 slide, a bone > marrow block, with specials and unstained slides=20 slides > > 2)Divide the number of slides to be cut by the number of cutters. > > 3)Distribute the blocks equally based on number of *slides *to be cut per > tech. Now obviously, you're going to want to give your biopsies, recuts, > specials and IHCs to your quickest cutters, and on down the line, in order > of priority, to the slower cutters. If you only have 2 cutters, one person > is going to cut all the bxs and specials and a few routines, and the other > person will cut the bulk of the routines. > > This daily routine achieves 3 things. > > 1) It keeps the slower cutters from slowing down your more critical > workflow. > 2) It removes any benefit from slacking, because it attempts to ensure > that everyone is doing the same amount of work, at least as far as cutting > goes. > 3) Most importantly, it identifies and isolates anyone who is cutting > slower, because they will still be sitting there cutting while everyone > else is done. > > While this might seem cruel, most humans are very group oriented. If the > slower cutters are experienced cutter who are sandbagging, they will > usually pick up their pace. > > If someone is inexperienced and trying to get faster, that's OK, and you > can focus on trying to help them get faster. > > A very small minority of people might be happy to sit there and cut their > routines as slowly as possible, and you have to decide whether or not to > start the process of getting rid of people like that. At least dividing the > cutting like this avoids them slowing down your workflow too much. > > You can carrot and stick this however you want. I see you are a histology > supervisor, so I'm assuming it's actually stick/stick. (j/k :)) Maybe not > let anyone go to lunch until all the cutting is done? Let the fastest > cutters leave 30 min early on a Friday? Send a clear message that it's not > OK just to slack, you want everyone working to their potential. > > > > > > > > On Fri, Jul 5, 2019 at 12:59 PM WILLIAM DESALVO via Histonet < > histonet at lists.utsouthwestern.edu <%0Dhistonet at lists.utsouthwestern.edu>> wrote: > > > I suggest you use slides created for the microtomy minimum standard. Not > > all blocks are created equal. A good target is 30 slides per hour, for > > mixed specimens. If all specialty, then adjust from the 30. > > > > William DeSalvo > > ________________________________ > > From: Pairan, Kelly via Histonet > > Sent: Friday, July 5, 2019 8:41:28 AM > > To: histonet at lists.utsouthwestern.edu <+histonet at lists.utsouthwestern.edu> > > Subject: [Histonet] Productivity Tracking > > > > Good Morning Histoland, > > How are you tracking your histotechs productivity when it comes to > > cutting? We recently have implemented a 25 block per hour goal for all of > > our histotechs and are receiving some push back. I made 25 block per hour > > the goal based on the following article that has been circulating for many > > years ( > > https://www.researchgate.net/publication/41942535_Productivity_standards_for_histology_laboratories). > > While I do not want to compromise quality, we do have turnaround times to > > meet and I have experienced techs who are cutting less than 20 blocks per > > hour. I understand that some tissues and protocols take longer so this is > > an average not something that has to be hit every shift. > > > > Thanks, > > Kelly > > > > Kelly Pairan, HT (ASCP)CM, QIHC (ASCP) > > Histology Supervisor-Anatomic Pathology > > Department of Pathology and Laboratory Medicine > > Email: kelly.pairan at nationwidechildrens.org <+kelly.pairan at nationwidechildrens.org> > > ph: 614-722-5414 > > fx: 614-722-3033 > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu <+Histonet at lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu <+Histonet at lists.utsouthwestern.edu> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > From wdesalvo.cac at outlook.com Sun Jul 7 19:49:13 2019 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Mon, 8 Jul 2019 00:49:13 +0000 Subject: [Histonet] Productivity Tracking In-Reply-To: References: , Message-ID: I think there is a MOST important aspect left out of this discussion. . . QUALITY. The point is never how fast you can perform a task, but rather at what pace can you consistently produce quality work. Inferior or unacceptable slides will increase cost, in time and money, 4 to 10 times. Quality and productivity goals MUST be strongly linked or the patient suffers!!! William DeSalvo ________________________________ From: Jay Lundgren Sent: Sunday, July 7, 2019 1:20:56 PM To: jasonhauser71 at gmail.com Cc: WILLIAM DESALVO; Pairan, Kelly; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Productivity Tracking As I pointed out, management can incentivize this any way they want. You could give raises, or you could say a tech is not eligible for a raise until they can cut at a certain rate. The idea is to bring the slower cutters up to speed, not get rid of them. Based on my experience, if you isolate and identify the malingerers, they will most likely speed up of their own accord. Most people will stop their BS once they realize everyone is on to them. The majority of people like to belong to a group, and don't like to be thought of as being bad at their job. Only if they absolutely refuse to try to speed up would you start the process of getting rid of them, and only in certain situations. We're talking the truly toxic employee. At least this way, the slower cutters don't slow down your whole workflow, and it attempts to ensure some fairness in the amount of cutting each tech does. Also, you don't have to burden the faster cutters with more paperwork for productivity reporting, not to mention the hours it will take for management to collate and interpret the data. It just takes someone counting the slides daily, which takes a couple of minutes tops. But your comment is a good example. Eventually, if everyone is not pulling together, the faster techs will start feeling taken advantage of, and change their own attitude towards work as well. Why would you feel that you are "getting saddled" with critical work, rather than feeling proud that management has recognized your talent and is utilizing it efficiently to improve patient care? Do you feel like its more work to cut 60 slides of bxs, specials, and IHCs (say, 10 blocks) than 60 slides of routine surgicals (60 blocks)? Personally, I'd rather cut the bxs. On Sun, Jul 7, 2019 at 2:02 PM jasonhauser71 at gmail.com > wrote: Really? If I am the faster cutter getting saddled with the more critical work, I would expect better pay than the slower cutter relegated to routine work. If one managesfrom the standpoint of individual productivity- one will never be satisfied. The slowest and least produ tive can be fired or laid off...that just means there is a new victim Jason Hauser Sr. Histology Tecnician, MLT The South Bend Clinic South Bend Indiana ------ Original message------ From: Jay Lundgren Date: Sat, Jul 6, 2019 2:12 PM To: WILLIAM DESALVO; Cc: Pairan, Kelly;histonet at lists.utsouthwestern.edu; Subject:Re: [Histonet] Productivity Tracking By the way, the standard for graduation from AFIP was 30 blocks/hr. Don't count blocks, count *slides*. If you have people slacking, you really need to start action to get rid of them. One rotten apple ruins the whole bunch. Google the new research on toxic personalities in the workplace. But if you want to try to bring them up to speed, try this: (Assuming one processing run, but the principle still applies.) 1)Someone takes the day's worksheet, and counts the number of slides to be cut, including recuts, special stains, IHC's and everything. So, according to your protocol, (numbers made up for example) 1 GI block=3 slides, an IHC panel= 10 slides, a routine tonsil block=1 slide, a bone marrow block, with specials and unstained slides=20 slides 2)Divide the number of slides to be cut by the number of cutters. 3)Distribute the blocks equally based on number of *slides *to be cut per tech. Now obviously, you're going to want to give your biopsies, recuts, specials and IHCs to your quickest cutters, and on down the line, in order of priority, to the slower cutters. If you only have 2 cutters, one person is going to cut all the bxs and specials and a few routines, and the other person will cut the bulk of the routines. This daily routine achieves 3 things. 1) It keeps the slower cutters from slowing down your more critical workflow. 2) It removes any benefit from slacking, because it attempts to ensure that everyone is doing the same amount of work, at least as far as cutting goes. 3) Most importantly, it identifies and isolates anyone who is cutting slower, because they will still be sitting there cutting while everyone else is done. While this might seem cruel, most humans are very group oriented. If the slower cutters are experienced cutter who are sandbagging, they will usually pick up their pace. If someone is inexperienced and trying to get faster, that's OK, and you can focus on trying to help them get faster. A very small minority of people might be happy to sit there and cut their routines as slowly as possible, and you have to decide whether or not to start the process of getting rid of people like that. At least dividing the cutting like this avoids them slowing down your workflow too much. You can carrot and stick this however you want. I see you are a histology supervisor, so I'm assuming it's actually stick/stick. (j/k :)) Maybe not let anyone go to lunch until all the cutting is done? Let the fastest cutters leave 30 min early on a Friday? Send a clear message that it's not OK just to slack, you want everyone working to their potential. On Fri, Jul 5, 2019 at 12:59 PM WILLIAM DESALVO via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I suggest you use slides created for the microtomy minimum standard. Not > all blocks are created equal. A good target is 30 slides per hour, for > mixed specimens. If all specialty, then adjust from the 30. > > William DeSalvo > ________________________________ > From: Pairan, Kelly via Histonet > Sent: Friday, July 5, 2019 8:41:28 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Productivity Tracking > > Good Morning Histoland, > How are you tracking your histotechs productivity when it comes to > cutting? We recently have implemented a 25 block per hour goal for all of > our histotechs and are receiving some push back. I made 25 block per hour > the goal based on the following article that has been circulating for many > years ( > https://www.researchgate.net/publication/41942535_Productivity_standards_for_histology_laboratories). > While I do not want to compromise quality, we do have turnaround times to > meet and I have experienced techs who are cutting less than 20 blocks per > hour. I understand that some tissues and protocols take longer so this is > an average not something that has to be hit every shift. > > Thanks, > Kelly > > Kelly Pairan, HT (ASCP)CM, QIHC (ASCP) > Histology Supervisor-Anatomic Pathology > Department of Pathology and Laboratory Medicine > Email: kelly.pairan at nationwidechildrens.org > ph: 614-722-5414 > fx: 614-722-3033 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ronald.kusters at pfmmedical.com Mon Jul 8 01:50:43 2019 From: ronald.kusters at pfmmedical.com (Kusters, Ronald) Date: Mon, 8 Jul 2019 06:50:43 +0000 Subject: [Histonet] HSV-2 antibody - Cell Marque was not the problem In-Reply-To: References: <3CEB8EBCF9C7A648B9694B5696462A71A665D104@NT-EX1.wvuhs.com> Message-ID: Hi Beth, I can only fully agree to the statement below. Well done Ronald Mit freundlichen Gr??en/Kind Regards Ronald Kusters European & Global Sales Manager Feather Brand Histotechnology pfm medical ag Wankelstra?e 60 50996 K?ln, Germany T +49 (2236) 9641 660 F +49 (2236) 9641 99 660 M +49 (171) 9144343 -----Original Message----- From: Sheeder, Christopher [mailto:Christopher.Sheeder at seattlechildrens.org] Sent: 05 July 2019 17:56 To: O'Neil, Beth ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] HSV-2 antibody - Cell Marque was not the problem Beth, You have honor and integrity. Something seldom seen these days. Chris Sheeder Seattle Children's Hospital -----Original Message----- From: O'Neil, Beth Sent: Wednesday, July 03, 2019 11:20 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] HSV-2 antibody - Cell Marque was not the problem Last week I posted an inquiry about having problems with Cell Marque's HSV-2 polyclonal antibody. I have to retract my statement that it was Cell Marque using a different antibody source. After spending two weeks troubleshooting my stainer, troubleshooting the positive control slides, yelling at my Cell Marque rep, etc. I found out that it was the positive control slide and not the antibody. When I originally suspected the positive QC slides (from Cancer Diagnostics), I requested a different lot number. This new lot also failed to show positive staining. Long story short, another call to Cancer Diagnostics finally resulted in receiving confirmation that they are having problems with their HSV-2 control slides. It was my misfortune to have opened a new box of QC slides at the same time as opening a new vial of HSV-2 antibody which resulted in two weeks of headaches. I will say that Cell Marque/Millipore was very supportive through this. So, for those of you who are using Cancer Diagnostics HSV-2 control slides, they are working on a resolution and will replace their "bad" slides. I was told that the staining is "hit or miss, " hopefully you all have the "hit" slides. Beth Oneil, WVU Medicine Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From estevenson at ariadxs.com Mon Jul 8 13:15:09 2019 From: estevenson at ariadxs.com (Emily Stevenson) Date: Mon, 8 Jul 2019 14:15:09 -0400 Subject: [Histonet] cryostat vs sliding microtome Message-ID: Hello, Looking for information on using a cryostat vs a sliding microtome for ENFD/small nerve fiber tissue cutting. Thanks! -- -- Confidentiality Notice: The information included in this e-mail, including any attachments, is for the sole use of the intended recipient and may contain information that is confidential and protected. Any unauthorized review, use, disclosure, distribution or similar action is prohibited. If you are not the intended recipient, please contact the sender and delete all copies of the original message immediately. From estevenson at ariadxs.com Mon Jul 8 13:17:50 2019 From: estevenson at ariadxs.com (Emily Stevenson) Date: Mon, 8 Jul 2019 14:17:50 -0400 Subject: [Histonet] ENFD/Small nerve fiber processing training Message-ID: Hello, Looking for information on ENFD/Small nerve fiber tissue processing for PGP9.5 staining. Any information would be greatly appreciated! Also does anyone know where I could receive training for this tissue process? Thanks -- -- Confidentiality Notice: The information included in this e-mail, including any attachments, is for the sole use of the intended recipient and may contain information that is confidential and protected. Any unauthorized review, use, disclosure, distribution or similar action is prohibited. If you are not the intended recipient, please contact the sender and delete all copies of the original message immediately. From richard.wild at sfr.fr Tue Jul 9 09:02:03 2019 From: richard.wild at sfr.fr (richard.wild at sfr.fr) Date: Tue, 9 Jul 2019 16:02:03 +0200 Subject: [Histonet] Shandon cryostat FSE cryocassette Message-ID: Hi - I could be interested for old cryocassette 25mm or other size for personal purpoise? - Do you have any to sell ? Best regards Ric --- L'absence de virus dans ce courrier ?lectronique a ?t? v?rifi?e par le logiciel antivirus Avast. https://www.avast.com/antivirus From craigak12 at gmail.com Tue Jul 9 14:27:29 2019 From: craigak12 at gmail.com (J B) Date: Tue, 9 Jul 2019 12:27:29 -0700 Subject: [Histonet] Bone marrow kit boxes: Message-ID: Can someone please recommend a good place to order customizable bone marrow biopsy kits? We want foam for specimen containers, tubes, and want to add our own products. Not premade completely. Thank you, JB From PREISZNE at mail.etsu.edu Thu Jul 11 09:13:59 2019 From: PREISZNE at mail.etsu.edu (Preiszner, Johanna) Date: Thu, 11 Jul 2019 14:13:59 +0000 Subject: [Histonet] StatLab paraffins? Message-ID: Hi, does anybody use StatLab paraffin for embedding? If so which one, and what is your experience using it? Their pricing looks good, so I'd like to try. Thanks, Hanna Preiszner ETSU/QCOM From relia1 at earthlink.net Thu Jul 11 11:24:33 2019 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 11 Jul 2019 12:24:33 -0400 Subject: [Histonet] ICYMI: Here is a link to my latest article Misconceptions about Relocation - And Our Current Histology Opportunities!! Message-ID: <009401d53805$20601f60$61205e20$@earthlink.net> Hi Histonetters, ICYMI - In case you missed it!! My latest article has been published in the NSH Quarterly Career Center Newsletter under my byline: Make the Cut and on the Fixation My latest article is entitled: Misconceptions about Relocation and some tough love for my histopeeps. This is the link to my article on the NSH's Fixation on Histology Blog and it is also in the current edition of the NSH Career Newsletter. Here is the link to the article: https://www.fixationonhistology.com/post/misconceptions-about-relocation-and -some-tough-love-for-my-histopeeps If you have a minute to read it I would love to hear what you think. If you can't get to the article with this link let me know and I will send you a copy of it. We have histology opportunities nationwide! Here are a few of the locations with new positions. Histology Spotlight Opportunities: I am recruiting for IHC Specialists in: >>Atlanta, GA >>Richmond, VA >>Greater NYC (No NY Lic required for this position!) >>Philadelphia, PA Help me help you help your histopeeps with their IHC challenges!! I also have great histology opportunities in the following areas: HT HTL or ASCP Elig! Florida Panama City - Days private lab California S.F. Bay area - private lab - days!! California Chico - nights private lab California San Diego - Days - private lab! Virginia Roanoke - Days!! Wisconsin Milwaukee - Days! North Carolina Greensboro - Days! North Carolina Charlotte - Nights My clients offer competitive pay rates, excellent benefits and in most cases relocation assistance and or a sign on bonus. If You or Anyone You Know Might Be Interested In a New Opportunity, Please Contact Me ASAP If you want to chat ASAP call or text me on my cell at 407-353-5070. If you want some additional information or to set up a time to chat please call me toll free at 866-607-3542 or email me at relia1 at earthlink.net My clients are ready to interview and hire right away!!! Have a great day. I look forward to hearing back from you. Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From SteveM at mcclainlab.com Thu Jul 11 15:06:08 2019 From: SteveM at mcclainlab.com (Steve McClain) Date: Thu, 11 Jul 2019 20:06:08 +0000 Subject: [Histonet] Histonet Digest, Vol 188, Glass plate for AO knife sharpener In-Reply-To: References: Message-ID: <0BEC3FE9-9FCA-48FF-8D8E-82A3B2466DF9@mcclainlab.com> Does anyone know where to find the glass plate for an ancient AO sharpener for microtome? Steve Steve A. McClain, MD 631-361-4000 Cell 631-926-3655 From bonnie_salyer at tedpella.com Thu Jul 11 16:42:15 2019 From: bonnie_salyer at tedpella.com (Bonnie Salyer) Date: Thu, 11 Jul 2019 14:42:15 -0700 Subject: [Histonet] Career Opportunity Message-ID: <021801d53831$821efec0$865cfc40$@tedpella.com> Ted Pella Inc. is seeking candidates for a Life Sciences Product Specialist position. We are looking for a someone with a strong background in Histology who also has a strong background in Electron Microscopy (specifically TEM) - both instrument use and specimen prep experience. If this describes you (or someone you know), please see our job posting on our website for complete details https://www.tedpella.com/company_html/Careers.aspx Bonnie Salyer, SPHR, SHRM-SCP Human Resources Manager Ted Pella, Inc. Phone: 530-243-2200 x260 Fax: 530-243-8278 bonnie_salyer at tedpella.com www.tedpella.com From Valerie.Hannen at parrishmed.com Fri Jul 12 04:56:55 2019 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Fri, 12 Jul 2019 05:56:55 -0400 Subject: [Histonet] [EXTERNAL Sender] Re: Histonet Digest, Vol 188, Glass plate for AO knife sharpener In-Reply-To: <0BEC3FE9-9FCA-48FF-8D8E-82A3B2466DF9@mcclainlab.com> References: <0BEC3FE9-9FCA-48FF-8D8E-82A3B2466DF9@mcclainlab.com> Message-ID: <450B7A81EDA0C54E97C53D60F00776C326670C3076@isexstore03> Unfortunately, I tried to find some as well and they no longer are available. Also, a heads up, I no longer can find fine abrasive either, all of the vendors that I contacted said they no longer make it. So, as soon as the fine abrasive I have is gone I will be switching over to disposable blades. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Steve McClain via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, July 11, 2019 4:06 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL Sender] Re: [Histonet] Histonet Digest, Vol 188, Glass plate for AO knife sharpener This message came from an external source. Please do not click links or open attachments if unexpected or unusual. Begin Original Message: ---------------------------------------------------------------------- Does anyone know where to find the glass plate for an ancient AO sharpener for microtome? Steve Steve A. McClain, MD 631-361-4000 Cell 631-926-3655 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=Xk0H5Lt1SX4yLDi3Z36FWCjxWMexCFmv9kQz5nprqM4&r=S0NRG57vjoVygxIXrxrniUhxWkoaXBwDrgVZ_u7KUmY&m=o3rXMMNNrKqn2L-HQGQrODMcQpAvPi-E_IL2RWXoUcM&s=Fv-BnggmUPV3-dX_N3jb3nVtPPFH6vTnwWo6zsFTcZ4&e= From CDavis at che-east.org Fri Jul 12 09:45:30 2019 From: CDavis at che-east.org (Cassie P. Davis) Date: Fri, 12 Jul 2019 14:45:30 +0000 Subject: [Histonet] Special Stain Wash Message-ID: Happy Friday Histoland, I realize we've had quite a bit of discussion over the past couple of years about the Special Stain Wash not lasting as long and could possibly be why sometimes the reason the silvers don't turn out as nice as they used to on the NEXES special stainer. My question is, has anyone tried making the working solution from the concentrate in smaller batches and does it help or does the concentrate seem to expire as soon as you open it? It seems to affect only our GMS, the other stains seem fine. It's been a challenge to try to figure it out. Even though my boss put the new stainer on the budget request, I still want to give our best care. Thanks in advance for sharing you experience. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From relia1 at earthlink.net Mon Jul 15 11:43:38 2019 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 15 Jul 2019 12:43:38 -0400 Subject: [Histonet] Dermatopathology Histology Opportunity Full Time Permanent Days In Columbus, GA A RELIA Exclusive ! Message-ID: <012601d53b2c$745699d0$5d03cd70$@earthlink.net> Hi Histopeeps! Happy Monday! I have been engaged exclusively by a private Dermatopathology lab to find a fantastic histotech to work as the sole practioner in this state of the art lab. This is a full time permanent M-F day shift position and my client offers a great pay rate, nice benefits and an excellent environment to work in. For more information please contact me ? Pam Barker at relia1 at earthlink.net or asap on my cell/text at 407-353-5070 or toll free at the office at 866-607-3542. If you refer someone to me and I place them you will earn a referral fee! Have a great day! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From relia1 at earthlink.net Mon Jul 15 12:19:46 2019 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 15 Jul 2019 13:19:46 -0400 Subject: [Histonet] Dermatopathology Histology Opportunity Full Time Permanent Days In Columbus, GA A RELIA Exclusive ! Message-ID: <012f01d53b31$80038bd0$800aa370$@earthlink.net> Hi Histopeeps! Reposting - because I only stated the location in the subject line... Happy Monday! I have been engaged exclusively by a private Dermatopathology lab in Columbus GA to find a fantastic histotech to work as the sole practioner in this state of the art lab. This is a full time permanent M-F day shift position and my client offers a great pay rate, nice benefits and an excellent environment to work in. For more information please contact me ? Pam Barker at relia1 at earthlink.net or asap on my cell/text at 407-353-5070 or toll free at the office at 866-607-3542. If you refer someone to me and I place them you will earn a referral fee! Have a great day! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From naje1972 at yahoo.com Mon Jul 15 14:03:26 2019 From: naje1972 at yahoo.com (cynthia haynes) Date: Mon, 15 Jul 2019 19:03:26 +0000 (UTC) Subject: [Histonet] Dermatopathology Histology Opportunity Full Time In-Reply-To: <012601d53b2c$745699d0$5d03cd70$@earthlink.net> References: <012601d53b2c$745699d0$5d03cd70$@earthlink.net> Message-ID: <488138009.2242409.1563217406598@mail.yahoo.com> Sent from Yahoo Mail on Android On Mon, Jul 15, 2019 at 11:56 AM, Pam Barker via Histonet wrote: Hi Histopeeps! Happy Monday! I have been engaged exclusively by a private Dermatopathology lab to find a fantastic histotech to work as the sole practioner in this state of the art lab. This is a full time permanent M-F day shift position and my client offers a great pay rate, nice benefits and an excellent environment to work in. For more information please contact me ? Pam Barker at relia1 at earthlink.net or asap on my cell/text at 407-353-5070 or toll free at the office at 866-607-3542. If you refer someone to me and I place them you will earn a referral fee!? Have a great day! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague at pathologyarts.com Mon Jul 15 16:37:23 2019 From: c.tague at pathologyarts.com (Curt Tague) Date: Mon, 15 Jul 2019 21:37:23 +0000 Subject: [Histonet] hair analysis question Message-ID: This isn't directly related to histology but I know many of you are involved in other studies as well so I thought I'd ask. We're working with some physicians on a hair analysis project, looking at some genetics. Does anyone here have any background in hair analysis for more than the forensic or drug screening utility? Is there any other test that you are familiar with, done from hair, or the pulp, that offers some prognostic or diagnostic utility? Thanks if you do! Curt From shultz11 at cox.net Wed Jul 17 08:38:02 2019 From: shultz11 at cox.net (shultz11) Date: Wed, 17 Jul 2019 08:38:02 -0500 Subject: [Histonet] Job Message-ID: Histology job a t Louisiana State University in Baton Rouge, LA. Must have IHC experience.Thanks. Kendra Shultz225-578-9724Sent from my Verizon, Samsung Galaxy smartphone From DKnutson at primecare.org Wed Jul 17 09:21:49 2019 From: DKnutson at primecare.org (Knutson, Deanne) Date: Wed, 17 Jul 2019 09:21:49 -0500 Subject: [Histonet] Tissue Adherence Issue Message-ID: <1E0E2B14C709174B8AC2BE0AE7F768330159B5756408@EXCHANGE2K7.staprimecare.org> Fellow Histonetters - I would appreciate your feedback on an intermittent issue that has shown up in our lab. All of a sudden, we are having difficulty with tissue specimens falling off of our slides on the IHC stains and special stains sporadically. About a year ago, we switched instruments on the IHC bench from the Leica BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer. No adherence issues with all of the validation slides that were run. We have tried various types of slides recently as well, and the issue still prevails. Would any of you mind telling me your slide workflow - type of slide used, gelatin or not used in flotation bath, how long slides are cooked, etc.... for your IHC slides, for your special stains slides, and even for your H&E slides. I would welcome your suggestions and feedback. Thank you so much!!! Deanne Knutson Supervisor Anatomic Pathology [X] "Let All Be Received as Christ." ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From relia1 at earthlink.net Wed Jul 17 09:43:17 2019 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 17 Jul 2019 10:43:17 -0400 Subject: [Histonet] Histology Opportunities available in Atlanta and Columbus GA. Message-ID: <09b501d53cad$f8a6fe00$e9f4fa00$@earthlink.net> Hello Histonetters, I hope you are having a great week! I have a couple of new positions to tell you about ? Both Opportunities are RELIA EXCLUSIVES!! These are permanent full time day shift positions offering excellent compensation and benefits. IHC Specialist ? Atlanta, GA Histotech ? Columbus, GA If you or anyone you know might be interested, please contact me. Histonetters, If I place someone you refer You will earn a referral fee! I can be reached toll free at the office at 866-607-3542 or relia1 at earthlink.net or you can always catch me on cell via call or text at 407-353-5070. Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Andrew.Dilts at coxhealth.com Wed Jul 17 12:06:45 2019 From: Andrew.Dilts at coxhealth.com (Dilts,Andrew) Date: Wed, 17 Jul 2019 17:06:45 +0000 Subject: [Histonet] Tissue Adherence Issue In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F768330159B5756408@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F768330159B5756408@EXCHANGE2K7.staprimecare.org> Message-ID: <8074597498c146ea9578ef5a85a477c4@coxhealth.com> We find that the amount of time slides spend baking in the oven prior to deparaffinization on our Benchmark Ultras tends to make a difference. Harder tissue like brain and bone can even benefit from 2 hours at 58*C. Andrew Dilts HTL(ASCP) Histo Supervisor, Laboratory Services Phone: (417) 269-5021 Andrew.Dilts at coxhealth.com www.coxhealth.com -----Original Message----- From: Knutson, Deanne Sent: Wednesday, July 17, 2019 9:22 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Tissue Adherence Issue Fellow Histonetters - I would appreciate your feedback on an intermittent issue that has shown up in our lab. All of a sudden, we are having difficulty with tissue specimens falling off of our slides on the IHC stains and special stains sporadically. About a year ago, we switched instruments on the IHC bench from the Leica BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer. No adherence issues with all of the validation slides that were run. We have tried various types of slides recently as well, and the issue still prevails. Would any of you mind telling me your slide workflow - type of slide used, gelatin or not used in flotation bath, how long slides are cooked, etc.... for your IHC slides, for your special stains slides, and even for your H&E slides. I would welcome your suggestions and feedback. Thank you so much!!! Deanne Knutson Supervisor Anatomic Pathology [X] "Let All Be Received as Christ." ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. CoxHealth ? ranked one of Missouri's Best Hospitals by U.S. News & World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intendedrecipient(s) and may contain confidential and privileged information protected by law. ?Any unauthorized review, use, disclosure or distribution is prohibited.?If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tbraud at holyredeemer.com Wed Jul 17 12:25:38 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 17 Jul 2019 17:25:38 +0000 Subject: [Histonet] Tissue Adherence problem Message-ID: <48E053DDF6CE074DB6A7414BA05403F8015707A2B6@HRHEX02-HOS.holyredeemer.local> Hi Deanne - Hope this will help For our Ventana Ultra, we use Leica / Surgipath Apex Superior Adhesive slides. We use de ionized water in our baths at 45C ish, no additives. We never "double-dip" our slides (meaning that if we mount a control on the top of the slide, we turn the slide upside down to pick it up, only immersing the slide to pick up the intended tissue) We pre-bake IHC slides in a hot air blower over at 65C, 10 min for small tissues, and 1 hr for large tissues, then load for IHC. For special stains, we use the same slides, but they go directly on the Sakura Prisma stainer into the instrument oven at 65C for 15 minutes before staining For H&E, we use the plain Surgipath Snowcoat slides, no adhesive, directly on the Sakura Prisma stainer into the instrument oven at 65C for 15 minutes before staining. They dry vertically in a rack as we cut, then we load as soon as the rack is full. We once had an issue with tissue falling off and were able to trace it to a new tech's liberal use of Paraguard Spray. Stopped using it, wiped everything down with alcohol and our problem went away. Also, many years ago, I had a problem with a bad lot of slides, but these were Cardinal's. I sincerely hope you can get this straightened out. I know how frustrating such a problem can be. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 2. Tissue Adherence Issue (Knutson, Deanne) Message: 2 Date: Wed, 17 Jul 2019 09:21:49 -0500 From: "Knutson, Deanne" Subject: [Histonet] Tissue Adherence Issue Fellow Histonetters - I would appreciate your feedback on an intermittent issue that has shown up in our lab. All of a sudden, we are having difficulty with tissue specimens falling off of our slides on the IHC stains and special stains sporadically. About a year ago, we switched instruments on the IHC bench from the Leica BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer. No adherence issues with all of the validation slides that were run. We have tried various types of slides recently as well, and the issue still prevails. Would any of you mind telling me your slide workflow - type of slide used, gelatin or not used in flotation bath, how long slides are cooked, etc.... for your IHC slides, for your special stains slides, and even for your H&E slides. I would welcome your suggestions and feedback. Thank you so much!!! Deanne Knutson Supervisor Anatomic Pathology "Let All Be Received as Christ." From kaarrington at anthc.org Wed Jul 17 12:46:34 2019 From: kaarrington at anthc.org (Arrington, Karla A) Date: Wed, 17 Jul 2019 17:46:34 +0000 Subject: [Histonet] HT Opening Message-ID: <458c3ea5b6054fa19c3f1a4bf146e266@anthc.org> Hello All, I hope everyone is enjoying summer. In beautiful Alaska, our hospital caters to the Alaskan Native population with their entire medical needs being met. Currently we have a Histology Technician position available in our pathology department. This position is M-F, no weekends and a rotating on-call schedule. This position comes with excellent benefits and a team work environment. This also offers relocation assistance for the correct candidate. Must be ASCP certified or eligible. For immediate consideration, email or FAX a current resume to kaarrington at anthc.org and it will be forwarded to our hiring manager. Also visit our website at anthc.org and select Careers to apply online. If you have questions regarding this position or how to submit an online application, please call ANTHC Human Resources at (907) 729-1301 or email careers at anthc.org. Karla Arrington, HT(ASCP) HIT(AHIMA) Pathology Supervisor Alaska Native Medical Center 4315 Diplomacy Drive Anchorage, AK 99508 Tele: 907-729-1810 Fax: 907-729-1226 From gentras at auburn.edu Wed Jul 17 16:04:53 2019 From: gentras at auburn.edu (Atoska Gentry) Date: Wed, 17 Jul 2019 21:04:53 +0000 Subject: [Histonet] mouse pituitary Message-ID: Hello, does anyone have a tried & proven method and/or pointers for mounting mouse pituitary for cryo sectioning which guarantees great cross sections? Thanks! ~Atoska Sent from Mail for Windows 10 From abtdhu at gmail.com Thu Jul 18 07:52:12 2019 From: abtdhu at gmail.com (Dorothy Hu) Date: Thu, 18 Jul 2019 08:52:12 -0400 Subject: [Histonet] Tissue Adherence Issue In-Reply-To: References: Message-ID: > > 2. Tissue Adherence Issue (Knutson, Deanne) > Hello, I think your testing tissue might be over or under fixed in formaldehyde fixative? You didn't tell what kind of tissue though. We deal with bone tissue which needs extensively bake (at 37, 46 degree over night and 58 one hour) and carefully handled. Superfrost Plus slides are used. Especially gentle heat for heat induced antigen retrieval. Try to use enzyme antigen retrieval as possible. Best luck. Dorothy Hu > Message: 2 > Date: Wed, 17 Jul 2019 09:21:49 -0500 > From: "Knutson, Deanne" > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: [Histonet] Tissue Adherence Issue > Message-ID: > < > 1E0E2B14C709174B8AC2BE0AE7F768330159B5756408 at EXCHANGE2K7.staprimecare.org> > > Content-Type: text/plain; charset="us-ascii" > > Fellow Histonetters - > > I would appreciate your feedback on an intermittent issue that has shown > up in our lab. > All of a sudden, we are having difficulty with tissue specimens falling > off of our slides on the IHC stains and special stains sporadically. > About a year ago, we switched instruments on the IHC bench from the Leica > BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer. > No adherence issues with all of the validation slides that were run. > We have tried various types of slides recently as well, and the issue > still prevails. > > Would any of you mind telling me your slide workflow - type of slide used, > gelatin or not used in flotation bath, how long slides are cooked, etc.... > for your IHC slides, for your special stains slides, and even for your H&E > slides. > I would welcome your suggestions and feedback. > > Thank you so much!!! > > Deanne Knutson > Supervisor > Anatomic Pathology > > [X] > "Let All Be Received as Christ." > > > From Vmcilhargy at bergenmed.com Thu Jul 18 12:23:21 2019 From: Vmcilhargy at bergenmed.com (Virginia McIlhargy) Date: Thu, 18 Jul 2019 17:23:21 +0000 Subject: [Histonet] Histonet Digest, Vol 188, Issue 14 In-Reply-To: References: Message-ID: Works for me! Virginia McIlhargy 466 Old Hook Rd Suite 1 Emerson, NJ 07630 p: 201-483-2687 d: f: 201-383-9700 http://www.bergenmed.com? This message (and any associated files) is intended only for the use of the individual or entity to which it is addressed and may contain information that is confidential. If you are not the intended recipient you are hereby notified that any dissemination, copying or distribution of this message, or files associated with this message, is strictly prohibited. If you have received this message in error, please notify us immediately by replying to the message and deleting it from your computer. -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, July 18, 2019 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 188, Issue 14 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From DKnutson at primecare.org Fri Jul 19 11:52:14 2019 From: DKnutson at primecare.org (Knutson, Deanne) Date: Fri, 19 Jul 2019 11:52:14 -0500 Subject: [Histonet] Tissue Adherence Issue In-Reply-To: <1E0E2B14C709174B8AC2BE0AE7F768330159B5756408@EXCHANGE2K7.staprimecare.org> References: <1E0E2B14C709174B8AC2BE0AE7F768330159B5756408@EXCHANGE2K7.staprimecare.org> Message-ID: <1E0E2B14C709174B8AC2BE0AE7F768330159B575641E@EXCHANGE2K7.staprimecare.org> Thank you to everyone who responded back to my posting. There were so many great suggestions and ideas for us to investigate. Who better to reach out to for help on issues than our peers?! - You are awesome! THANK YOU!!!!! Deanne Knutson Supervisor Anatomic Pathology dknutson at primecare.org "Let All Be Received as Christ." -----Original Message----- From: Knutson, Deanne via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, July 17, 2019 9:22 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Tissue Adherence Issue CAUTION: This email is not from a CHI source. Only click links or open attachments you know are safe. Please send as an attachment all spam/phishing and unusual emails to spam at catholichealth.net. ...................................................................... Fellow Histonetters - I would appreciate your feedback on an intermittent issue that has shown up in our lab. All of a sudden, we are having difficulty with tissue specimens falling off of our slides on the IHC stains and special stains sporadically. About a year ago, we switched instruments on the IHC bench from the Leica BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer. No adherence issues with all of the validation slides that were run. We have tried various types of slides recently as well, and the issue still prevails. Would any of you mind telling me your slide workflow - type of slide used, gelatin or not used in flotation bath, how long slides are cooked, etc.... for your IHC slides, for your special stains slides, and even for your H&E slides. I would welcome your suggestions and feedback. Thank you so much!!! Deanne Knutson Supervisor Anatomic Pathology [X] "Let All Be Received as Christ." ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=YFhW2PYwN3hsZhoCqLOPHsIEIPQ6qDXkZ40AlEYUG9c&r=HEssDIPSD1gvhLCPOWavMQclZsG-P6T9JqKPXgdEnF0&m=KVKhD0nPsfF4mSH2out3qI_QbpLWEWSMqJD2c9plYe4&s=lmddTuDwCBd1RGTisDb0ymW0bgm0ne1cRTBN0Az2XAo&e= This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. From jaylundgren at gmail.com Fri Jul 19 13:14:05 2019 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 19 Jul 2019 13:14:05 -0500 Subject: [Histonet] Histonet Digest, Vol 188, Glass plate for AO knife sharpener In-Reply-To: <0BEC3FE9-9FCA-48FF-8D8E-82A3B2466DF9@mcclainlab.com> References: <0BEC3FE9-9FCA-48FF-8D8E-82A3B2466DF9@mcclainlab.com> Message-ID: How old is that thing? Reagan administration? They don't make 'em like that anymore. On Thu, Jul 11, 2019 at 3:13 PM Steve McClain via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Does anyone know where to find the glass plate for an ancient AO sharpener > for microtome? > > Steve > Steve A. McClain, MD > 631-361-4000 Cell 631-926-3655 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From john.garratt at ciqc.ca Fri Jul 19 15:49:46 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Fri, 19 Jul 2019 20:49:46 +0000 Subject: [Histonet] Tissue Adherence problem In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8015707A2B6@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8015707A2B6@HRHEX02-HOS.holyredeemer.local> Message-ID: <0597oAbSV8X3ciP-ZbwcND6ULi52ro0-cAvpBG8E_7hA3Qc2QpDm007eLiaY0iMtkw9IioaPs1iudyG4qrH5E6bcaTvhgpzv9pXUQ_pQ5RM=@ciqc.ca> Terri is right on the money with her advice. Especially the de- ionized water, NO double dipping and defiantly NO adhesive part. John Sent from ProtonMail Mobile On Wed, Jul 17, 2019 at 10:25 AM, Terri Braud via Histonet wrote: > Hi Deanne - > Hope this will help > For our Ventana Ultra, we use Leica / Surgipath Apex Superior Adhesive slides. > We use de ionized water in our baths at 45C ish, no additives. > We never "double-dip" our slides (meaning that if we mount a control on the top of the slide, we turn the slide upside down to pick it up, only immersing the slide to pick up the intended tissue) > We pre-bake IHC slides in a hot air blower over at 65C, 10 min for small tissues, and 1 hr for large tissues, then load for IHC. > > For special stains, we use the same slides, but they go directly on the Sakura Prisma stainer into the instrument oven at 65C for 15 minutes before staining > For H&E, we use the plain Surgipath Snowcoat slides, no adhesive, directly on the Sakura Prisma stainer into the instrument oven at 65C for 15 minutes before staining. They dry vertically in a rack as we cut, then we load as soon as the rack is full. > > We once had an issue with tissue falling off and were able to trace it to a new tech's liberal use of Paraguard Spray. Stopped using it, wiped everything down with alcohol and our problem went away. > Also, many years ago, I had a problem with a bad lot of slides, but these were Cardinal's. > I sincerely hope you can get this straightened out. I know how frustrating such a problem can be. > Terri > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Today's Topics: > 2. Tissue Adherence Issue (Knutson, Deanne) > Message: 2 > Date: Wed, 17 Jul 2019 09:21:49 -0500 > From: "Knutson, Deanne" > Subject: [Histonet] Tissue Adherence Issue > Fellow Histonetters - > > I would appreciate your feedback on an intermittent issue that has shown up in our lab. > All of a sudden, we are having difficulty with tissue specimens falling off of our slides on the IHC stains and special stains sporadically. > About a year ago, we switched instruments on the IHC bench from the Leica BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer. > No adherence issues with all of the validation slides that were run. > We have tried various types of slides recently as well, and the issue still prevails. > > Would any of you mind telling me your slide workflow - type of slide used, gelatin or not used in flotation bath, how long slides are cooked, etc.... for your IHC slides, for your special stains slides, and even for your H&E slides. > I would welcome your suggestions and feedback. > > Thank you so much!!! > Deanne Knutson > Supervisor > Anatomic Pathology > "Let All Be Received as Christ." > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sandra.cheasty at wisc.edu Tue Jul 23 14:49:21 2019 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Tue, 23 Jul 2019 19:49:21 +0000 Subject: [Histonet] Jeepers Creepers, Coverslipping Peepers! Message-ID: Hello all, Does anyone have a source for oversized coverglass? (35x50, 35x60, 45x50, 45x60) We section a lot of globes from the ophthalmology lab, some of which are from horses, cows, etc. that are put on the larger slides. Fisher used to carry their own brand, but it's apparently discontinued them, and their "alternate" product is $50/oz! Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From eddessa at emory.edu Tue Jul 23 15:46:38 2019 From: eddessa at emory.edu (Dessasau III, Evan) Date: Tue, 23 Jul 2019 20:46:38 +0000 Subject: [Histonet] [External] Jeepers Creepers, Coverslipping Peepers! In-Reply-To: References: Message-ID: Hi Sandra, Have you tried Brain Research Laboratories? We use the 35x50mm no.1, 3oz. pack. We buy them through Mark at PathSupply. The phone number on the box is 617 965 5544. PathSupply's number is 800 631 3556. Thank you, E-van -----Original Message----- From: Sandra Cheasty via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, July 23, 2019 3:49 PM To: Histonet (histonet at lists.utsouthwestern.edu) Subject: [External] [Histonet] Jeepers Creepers, Coverslipping Peepers! Hello all, Does anyone have a source for oversized coverglass? (35x50, 35x60, 45x50, 45x60) We section a lot of globes from the ophthalmology lab, some of which are from horses, cows, etc. that are put on the larger slides. Fisher used to carry their own brand, but it's apparently discontinued them, and their "alternate" product is $50/oz! Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From CDavis at che-east.org Wed Jul 24 11:41:14 2019 From: CDavis at che-east.org (Cassie P. Davis) Date: Wed, 24 Jul 2019 16:41:14 +0000 Subject: [Histonet] Two part Special Stain wash Message-ID: Hi Histonet folks, If you still have the NEXES special stainer do you use the two part wash cat#860-040 or the 10x concentrate cat#860-015? Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From gentras at auburn.edu Wed Jul 24 12:34:49 2019 From: gentras at auburn.edu (Atoska Gentry) Date: Wed, 24 Jul 2019 17:34:49 +0000 Subject: [Histonet] Histonet Digest, Vol 188, Issue 17 In-Reply-To: References: Message-ID: Hello Sandra, Following are catalog #'s for Fisher substitutes that I received from their site: 12-545GP (50x35mm); 12-548-5RP (45x50mm). Ted Pella, Inc. Gold Seal 3327 www.expressmedicalsupply.com Erie-Scientific ER 35x50-1.5 & ER 45x50-1 Also try Brain Research Laboratories 4860-1 (48x60mm) although they're a bit more pricey. Thomas Scientific (48x60mm) #6672-A64 Additionally, I've seen some eBay listings. Best wishes, ~Atoska -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Wednesday, July 24, 2019 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 188, Issue 17 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Jeepers Creepers, Coverslipping Peepers! (Sandra Cheasty) 2. Re: [External] Jeepers Creepers, Coverslipping Peepers! (Dessasau III, Evan) 3. Two part Special Stain wash (Cassie P. Davis) ---------------------------------------------------------------------- Message: 1 Date: Tue, 23 Jul 2019 19:49:21 +0000 From: Sandra Cheasty To: "Histonet (histonet at lists.utsouthwestern.edu)" Subject: [Histonet] Jeepers Creepers, Coverslipping Peepers! Message-ID: Content-Type: text/plain; charset="us-ascii" Hello all, Does anyone have a source for oversized coverglass? (35x50, 35x60, 45x50, 45x60) We section a lot of globes from the ophthalmology lab, some of which are from horses, cows, etc. that are put on the larger slides. Fisher used to carry their own brand, but it's apparently discontinued them, and their "alternate" product is $50/oz! Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ------------------------------ Message: 2 Date: Tue, 23 Jul 2019 20:46:38 +0000 From: "Dessasau III, Evan" To: Sandra Cheasty Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] [External] Jeepers Creepers, Coverslipping Peepers! Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Sandra, Have you tried Brain Research Laboratories? We use the 35x50mm no.1, 3oz. pack. We buy them through Mark at PathSupply. The phone number on the box is 617 965 5544. PathSupply's number is 800 631 3556. Thank you, E-van -----Original Message----- From: Sandra Cheasty via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, July 23, 2019 3:49 PM To: Histonet (histonet at lists.utsouthwestern.edu) Subject: [External] [Histonet] Jeepers Creepers, Coverslipping Peepers! Hello all, Does anyone have a source for oversized coverglass? (35x50, 35x60, 45x50, 45x60) We section a lot of globes from the ophthalmology lab, some of which are from horses, cows, etc. that are put on the larger slides. Fisher used to carry their own brand, but it's apparently discontinued them, and their "alternate" product is $50/oz! Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ------------------------------ Message: 3 Date: Wed, 24 Jul 2019 16:41:14 +0000 From: "Cassie P. Davis" To: histonet Subject: [Histonet] Two part Special Stain wash Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histonet folks, If you still have the NEXES special stainer do you use the two part wash cat#860-040 or the 10x concentrate cat#860-015? Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 188, Issue 17 ***************************************** From abijag76 at yahoo.co.in Thu Jul 25 08:32:32 2019 From: abijag76 at yahoo.co.in (abi jag) Date: Thu, 25 Jul 2019 13:32:32 +0000 (UTC) Subject: [Histonet] Troubleshooting Gomori's Trichrome Stain (Blue Collagen, Richard-Allen) staining References: <1905596166.444635.1564061552405.ref@mail.yahoo.com> Message-ID: <1905596166.444635.1564061552405@mail.yahoo.com> Dear histo experts,Please provide me with your valuable suggestions for the problem described below.Objective: Staining rat hearts (fibrosis) with?Gomori's one step Trichrome Stain (Blue Collagen, Richard-Allen) to demonstrate the collagenProcedure: Paraffin sections of 4 micron thickness; adequately fixed in 10 % NBF,??Bouin?s Fluid treatment at 56?C for 1 hour before staining. Follow the procedure exactly recommended by kit insert (please see below)Problem: Non specific diffuse bluish discoloration of cardio myocytes in the normal hearts, which looked completely odd. The staining of same section with picro sirius red came beautiful.Any insights on the potential reasons of this and ways to resolve?Thanks a lot in advance for your time and your vision to make a wealth of knowledge available in histonet. Best regards,Abi Standard Staining Protocol 1.?Deparaffinize and hydrate sections to deionized water. 2.?Place sections in Bouin?s Fluid at 56?C for 1 hour. 3.?Rinse sections in running tap water for 3-5 minutes untilyellow color is removed. 4.?Place sections in Working Weigert?s Iron Hematoxylin Stainfor 10 minutes. 5.?Rinse sections in running tap water for 5-10 minutes. 6.?Stain sections in Trichrome Stain for 15 minutes. 7.?Place sections in 1% Acetic Acid Solution for 1 minute. 8.?Rinse sections in deionized water for 30 seconds. 9.?Dehydrate sections in 95% alcohol for 1 minute. 10. Dehydrate sections in two changes of anhydrous alcohol for 1minute each. 11. Clear sections in three changes of clearing reagent for 1minute each and mount. From amyleehisto779 at gmail.com Thu Jul 25 14:12:38 2019 From: amyleehisto779 at gmail.com (Amy Lee) Date: Thu, 25 Jul 2019 12:12:38 -0700 Subject: [Histonet] Victoria Blue for lung tissue Message-ID: Hello Histonet, I was asked to do Victoria Blue stain on rodent FFPE lung tissue to exam thickness of artery. Could anybody recommend a good vendor of this reagent kit? Thanks, Amy From noreply-9499c78858b2038c5ee4bb593ca86912 at google.com Thu Jul 25 15:06:14 2019 From: noreply-9499c78858b2038c5ee4bb593ca86912 at google.com (Tina Van Meter (via Google Photos)) Date: Thu, 25 Jul 2019 13:06:14 -0700 Subject: [Histonet] Tina Van Meter shared 1 photo with you Message-ID: Google Photos Tina Van Meter shared a photo with you View photos here: https://photos.google.com/share/AF1QipOmPFQUdrwq38LWTOFG3NMGBO76eBIrtJ5RqSpJE4yPeRRisQqWxrdmczhonDuY-A?key=ODdVY3dXdnZ6X3ZNcXd3ckNVVkYyaTNic2phS1JR You received this mail because Tina Van Meter shared these photos with you. If you no longer wish to receive email notifications of shared photos, unsubscribe here: https://notifications.google.com/unsubscribe/AJ7SsMne1Yr6MB8uFES_tv8aiLB1fzEPVzrFuMPGyTdrjUSNnnEsu-tButowT2SSsV2pwjpPFn6tBAxsEur6xgZYcItc4Bp7opy0pzZMvowA0iIfDeiIsOFSIsDutWC-hUeVIyApErTbTt5J09wK-NJWUeLzEguTtw Get Google Photos (https://google.com/photos) Google LLC, 1600 Amphitheatre Pkwy, Mountain View, CA 94043 USA From noreply-9499c78858b2038c5ee4bb593ca86912 at google.com Thu Jul 25 15:09:53 2019 From: noreply-9499c78858b2038c5ee4bb593ca86912 at google.com (Tina Van Meter (via Google Photos)) Date: Thu, 25 Jul 2019 13:09:53 -0700 Subject: [Histonet] Tina Van Meter shared 1 photo with you Message-ID: Google Photos Tina Van Meter shared a photo with you View photos here: https://photos.google.com/share/AF1QipOKc_4x0eIUNrkkCTTA0kSVyMYpo5Y8Pi0I94PbvGGQ55kbNbvNqa_dqS3prA8ySA?key=WjlHXzY3Vl9yNTVnWWxDWWI0M2xZUHExbVcxR21B You received this mail because Tina Van Meter shared these photos with you. If you no longer wish to receive email notifications of shared photos, unsubscribe here: https://notifications.google.com/unsubscribe/AJ7SsMm2u-T1RyyRqqHF6VEIIvxM4che_8jqdSjuDsl_499hKdXq2XlgEGWRBCn1qpt1mAht4BTWvOGIUEqeBM0KT4Du9RqXnMlC0vbDwZYsuTIFLz9A-J8TZtEu0ZIcAqHJN5PLDz8RZaeC8iwmG8JkZCzuBGmR6w Get Google Photos (https://google.com/photos) Google LLC, 1600 Amphitheatre Pkwy, Mountain View, CA 94043 USA From noreply-9499c78858b2038c5ee4bb593ca86912 at google.com Thu Jul 25 15:10:44 2019 From: noreply-9499c78858b2038c5ee4bb593ca86912 at google.com (Tina Van Meter (via Google Photos)) Date: Thu, 25 Jul 2019 13:10:44 -0700 Subject: [Histonet] Tina Van Meter shared 1 photo with you Message-ID: <4UQZmpI2YytnSN89EsYBZw.0@notifications.google.com> Google Photos Tina Van Meter shared a photo with you View photos here: https://photos.google.com/share/AF1QipMn0txQiDfUXaAZsAvs9AgjU__Agh1OSklvgnCjkzq5mqKLmoD9aLjEZWh1M7g8rw?key=LWFxcTdsUy14R3Zha05GUXQtSGdWZkdJS2J1SFR3 You received this mail because Tina Van Meter shared these photos with you. If you no longer wish to receive email notifications of shared photos, unsubscribe here: https://notifications.google.com/unsubscribe/AJ7SsMlO3hpNRUsvAjpw-wXkvDnz9nqm9sugAjTE0tPf-999BeFb-Ipyaf3Ayv2XDRZbwkKDs-mr-IYULbVnhgT9tnVqAxiPTXzeDNfqmZhKo4n2J-XRQiK805_c8ibIee4VkzynxZyBU8ylutGZ2_7dqCqICCj-_Q Get Google Photos (https://google.com/photos) Google LLC, 1600 Amphitheatre Pkwy, Mountain View, CA 94043 USA From jkiernan at uwo.ca Thu Jul 25 15:34:30 2019 From: jkiernan at uwo.ca (John Kiernan) Date: Thu, 25 Jul 2019 20:34:30 +0000 Subject: [Histonet] Troubleshooting Gomori's Trichrome Stain (Blue Collagen, Richard-Allen) staining In-Reply-To: <1905596166.444635.1564061552405@mail.yahoo.com> References: <1905596166.444635.1564061552405.ref@mail.yahoo.com>, <1905596166.444635.1564061552405@mail.yahoo.com> Message-ID: One-step trichrome methods (such as Gomori's) are OK when they work, but there's little you can do when the colours come out wrong. A simple thing to try would be a shorter time in the staining mixture, to reduce diffusion of the more slowly penetrating dye (aniline blue) into cells. Trichrome methods work better after coagulant fixation than after formaldehyde. (The Bouin pre-treament is to offset the undesirable effects of fixation in neutral formaldehyde; picric acid alone works just as well. See also Yu & Chapman 2003 J. histotechnol. 26(2): 131-134.) If you can fix your hearts in Bouin or Carnoy you will get a better result with any trichrome technique. If the one-step method still won't work, use a multi-step trichrome where you can have some control over the actions of the different components. Masson's (which has several variants) is popular; Mallory's has fewer steps. Good luck! John Kiernan Anatomy, University of Western Ontario London, Canada = = = ________________________________ From: abi jag via Histonet Sent: 25 July 2019 08:32 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Troubleshooting Gomori's Trichrome Stain (Blue Collagen, Richard-Allen) staining Dear histo experts,Please provide me with your valuable suggestions for the problem described below.Objective: Staining rat hearts (fibrosis) with Gomori's one step Trichrome Stain (Blue Collagen, Richard-Allen) to demonstrate the collagenProcedure: Paraffin sections of 4 micron thickness; adequately fixed in 10 % NBF, Bouin?s Fluid treatment at 56?C for 1 hour before staining. Follow the procedure exactly recommended by kit insert (please see below)Problem: Non specific diffuse bluish discoloration of cardio myocytes in the normal hearts, which looked completely odd. The staining of same section with picro sirius red came beautiful.Any insights on the potential reasons of this and ways to resolve?Thanks a lot in advance for your time and your vision to make a wealth of knowledge available in histonet. Best regards,Abi Standard Staining Protocol 1.?Deparaffinize and hydrate sections to deionized water. 2.?Place sections in Bouin?s Fluid at 56?C for 1 hour. 3.?Rinse sections in running tap water for 3-5 minutes untilyellow color is removed. 4.?Place sections in Working Weigert?s Iron Hematoxylin Stainfor 10 minutes. 5.?Rinse sections in running tap water for 5-10 minutes. 6.?Stain sections in Trichrome Stain for 15 minutes. 7.?Place sections in 1% Acetic Acid Solution for 1 minute. 8.?Rinse sections in deionized water for 30 seconds. 9.?Dehydrate sections in 95% alcohol for 1 minute. 10. Dehydrate sections in two changes of anhydrous alcohol for 1minute each. 11. Clear sections in three changes of clearing reagent for 1minute each and mount. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - UT Southwestern Medical Center lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet From Richard.Cartun at hhchealth.org Thu Jul 25 15:48:15 2019 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Thu, 25 Jul 2019 20:48:15 +0000 Subject: [Histonet] Major skin resections - CPT coding Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EAC170D9A@HHCEXCHMB03.hhcsystem.org> I'm curious; how do you code major (complex) skin resections that also include soft tissue? These cases often have 10-30 paraffin blocks and lots of H&E slides. I'm thinking that they could be coded 88307 (simple resection) or 88309 (complex resection). I found out that our Dermatopathologist was coding these as "88305" and I don't think that is appropriate. Thank you in advance for your comments. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From jwwalker at rrmc.org Fri Jul 26 09:33:45 2019 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Fri, 26 Jul 2019 14:33:45 +0000 Subject: [Histonet] Major skin resections - CPT coding In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2EAC170D9A@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2EAC170D9A@HHCEXCHMB03.hhcsystem.org> Message-ID: Hi Dr. Cartun, We have had similar discussions about this in our institution, too. Unfortunately, according to our contracted lab compliance person, the correct code for all skin lesions is 88305. Below is an excerpt from Dennis Padget's coding manual (now owned by American Pathology Foundation) that provides information related to this topic: " Popular urban legend has it that it?s okay to report large or complex skin specimens?wide excisions, melanomas, and basal cell or Merkel cell with margins immediately come to mind?at the 88307 or 88309 charge level. Although these specimens unquestionably involve more work than lesser samples, what can?t be disputed is that they?re still very much accurately described by the label Skin, other than cyst/tag/debridement/plastic repair. Hence, you should ignore these myths. For added information on this important topic, refer to section 3 of chapter 7.) Report code 88305 unconditionally for a skin specimen diagnosed as other than a plastic repair, debridement, cyst, or tag. You can?t change the code based on the clinical diagnosis, pathologic diagnosis (other than to rule out the 88302 and 88304 categories), number of blocks or slides, a complication, or an extenuating circumstance." (APF, 2016, p.245) American Pathology Foundation. (2016). Pathology coding handbook (Ver. 16.3). Laguna Beach, CA: American Pathology Foundation. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Cartun, Richard via Histonet Sent: Thursday, July 25, 2019 4:48 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Major skin resections - CPT coding [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. I'm curious; how do you code major (complex) skin resections that also include soft tissue? These cases often have 10-30 paraffin blocks and lots of H&E slides. I'm thinking that they could be coded 88307 (simple resection) or 88309 (complex resection). I found out that our Dermatopathologist was coding these as "88305" and I don't think that is appropriate. Thank you in advance for your comments. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7Cd4801ff531f14be89d3c08d711418827%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C636996845467002275&sdata=wGn0MRRtj0BPI3nIMi0qDikP5uUU9OmoQv0sSDV7sKk%3D&reserved=0 [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] From relia1 at earthlink.net Fri Jul 26 10:14:02 2019 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 26 Jul 2019 11:14:02 -0400 Subject: [Histonet] TGIF Histopeeps! Run your own lab in Columbus, GA Message-ID: <000001d543c4$c354e730$49feb590$@earthlink.net> Hello Histopeeps, TGIF! I hope you are looking forward to a fantastic weekend. I have a new position to tell you about near Columbus, GA. This Is An Opportunity To Run Your Own Derm Histology Lab!! My client offers a great a.m. schedule, excellent compensation and relocation assistance. If you or anyone you know might be interested, when you get a chance please contact me. If I place someone you refer You will earn a referral fee! I can be reached toll free at the office at 866-607-3542 or relia1 at earthlink.net or you can always catch me ASAP on cell via call or text at 407-353-5070. Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From rsrichmond at gmail.com Fri Jul 26 13:10:40 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 26 Jul 2019 14:10:40 -0400 Subject: [Histonet] Victoria blue for lung tissue In-Reply-To: References: Message-ID: Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE lung tissue to exam thickness of artery. Could anybody recommend a good vendor of this reagent kit?<< You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and several others. I couldn't find anyone who offers a kit, only the dry dye. There are other dyes called Victoria Blue, reported to give the same results. You'd have to find a method for preparing it as an elastic stain, and I couldn't find such a method either with Google or in my old books. The requester needs to supply you with a method. I suspect the requester is reading an old article. There are stains for elastic tissue (which is I suppose what you want to "exam thickness of artery") that are a lot easier to get. Bob Richmond Samurai Pathologist Maryville TN From tony.henwood at health.nsw.gov.au Fri Jul 26 19:43:48 2019 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sat, 27 Jul 2019 00:43:48 +0000 Subject: [Histonet] Victoria blue for lung tissue In-Reply-To: References: , Message-ID: <1564188227985.76571@health.nsw.gov.au> Victoria Blue is the dye used in Miller's Stain for Elastic Tissue. It is also used in the Roche Ventana Benchmark Stainer to stain elastic Tissue: Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148?149 Karen Percival & Zaher Radi (2017) Comparison of five elastin histochemical stains to identify pulmonary small vasculature, Journal of Histotechnology, 40:3, 73-78 Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in Human Skin" Histologic 32(1): 12 Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 1996: 23: 37-42. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Bob Richmond via Histonet Sent: Saturday, July 27, 2019 4:10 AM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Victoria blue for lung tissue Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE lung tissue to exam thickness of artery. Could anybody recommend a good vendor of this reagent kit?<< You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and several others. I couldn't find anyone who offers a kit, only the dry dye. There are other dyes called Victoria Blue, reported to give the same results. You'd have to find a method for preparing it as an elastic stain, and I couldn't find such a method either with Google or in my old books. The requester needs to supply you with a method. I suspect the requester is reading an old article. There are stains for elastic tissue (which is I suppose what you want to "exam thickness of artery") that are a lot easier to get. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From tony.henwood at health.nsw.gov.au Sat Jul 27 16:53:13 2019 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sat, 27 Jul 2019 21:53:13 +0000 Subject: [Histonet] Victoria blue for lung tissue In-Reply-To: References: <1564188227985.76571@health.nsw.gov.au>, Message-ID: <1564264394036.68685@health.nsw.gov.au> Methods found here: https://www.ihcworld.com/_protocols/special_stains/miller's_elastic_ellis.htm http://www.histosearch.com/histonet/Dec98/Millersstainforelasticfib.html https://www.sakuraus.com/getattachment/774d89be-4b32-4d69-bb02-8dea75c52c0c/858 ? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________ From: Bob Richmond Sent: Saturday, July 27, 2019 11:27:54 AM To: Tony Henwood (SCHN) Subject: Re: [Histonet] Victoria blue for lung tissue Is this method published anywhere that "Amy" would likely have access to? This looked to me like one of those cases of the blind leading the blind that are all to common in research involving histochemistry. On Fri, Jul 26, 2019 at 8:44 PM Tony Henwood (SCHN) > wrote: Victoria Blue is the dye used in Miller's Stain for Elastic Tissue. It is also used in the Roche Ventana Benchmark Stainer to stain elastic Tissue: Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148-149 Karen Percival & Zaher Radi (2017) Comparison of five elastin histochemical stains to identify pulmonary small vasculature, Journal of Histotechnology, 40:3, 73-78 Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in Human Skin" Histologic 32(1): 12 Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 1996: 23: 37-42. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Bob Richmond via Histonet > Sent: Saturday, July 27, 2019 4:10 AM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Victoria blue for lung tissue Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE lung tissue to exam thickness of artery. Could anybody recommend a good vendor of this reagent kit?<< You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and several others. I couldn't find anyone who offers a kit, only the dry dye. There are other dyes called Victoria Blue, reported to give the same results. You'd have to find a method for preparing it as an elastic stain, and I couldn't find such a method either with Google or in my old books. The requester needs to supply you with a method. I suspect the requester is reading an old article. There are stains for elastic tissue (which is I suppose what you want to "exam thickness of artery") that are a lot easier to get. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From penny.marr at nhs.net Mon Jul 29 02:58:29 2019 From: penny.marr at nhs.net (MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST)) Date: Mon, 29 Jul 2019 07:58:29 +0000 Subject: [Histonet] Victoria blue for lung tissue In-Reply-To: <1564188227985.76571@health.nsw.gov.au> References: , <1564188227985.76571@health.nsw.gov.au> Message-ID: <3ea0f841254a4c49abd68c99b95e740b@NH-SLPEX113.AD1.NHS.NET> Miller's stain is available from VWR. I do not recommend the BSS to stain elastin - it is not strong enough and misses the fine fibres. Kind regards, Penny :) Penny Marr Senior BMS C/- Histology Conquest Hospital St Leonards-on-Sea TN37 7RD penny.marr at nhs.net (01424) 758023 -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood at health.nsw.gov.au] Sent: 27 July 2019 01:44 To: Bob Richmond Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Victoria blue for lung tissue Victoria Blue is the dye used in Miller's Stain for Elastic Tissue. It is also used in the Roche Ventana Benchmark Stainer to stain elastic Tissue: Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148-149 Karen Percival & Zaher Radi (2017) Comparison of five elastin histochemical stains to identify pulmonary small vasculature, Journal of Histotechnology, 40:3, 73-78 Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in Human Skin" Histologic 32(1): 12 Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 1996: 23: 37-42. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Bob Richmond via Histonet Sent: Saturday, July 27, 2019 4:10 AM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Victoria blue for lung tissue Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE lung tissue to exam thickness of artery. Could anybody recommend a good vendor of this reagent kit?<< You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and several others. I couldn't find anyone who offers a kit, only the dry dye. There are other dyes called Victoria Blue, reported to give the same results. You'd have to find a method for preparing it as an elastic stain, and I couldn't find such a method either with Google or in my old books. The requester needs to supply you with a method. I suspect the requester is reading an old article. There are stains for elastic tissue (which is I suppose what you want to "exam thickness of artery") that are a lot easier to get. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in relation to its contents. To do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland. NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and other accredited email services. For more information and to find out how you can switch, https://portal.nhs.net/help/joiningnhsmail From charles.rowlands at parioforma.com Mon Jul 29 06:21:41 2019 From: charles.rowlands at parioforma.com (Charles Rowlands) Date: Mon, 29 Jul 2019 12:21:41 +0100 Subject: [Histonet] Research on the Duopoly in PAP analysis equipment in the US Message-ID: <01d601d545ff$cbf648b0$63e2da10$@parioforma.com> Hello! We undertaking a research project about the technology used in automated PAP analysis in USA and would like to reach out to several US based histofolks who would like to share some opinions and expert views with us. We have a modest budget for an honorarium to compensate people for their time. If you would like to share your views and experiences please get in touch with me at your earliest convenience. Key issues we would like to explore: "Today the US PAP testing market is dominated by BD SurePath and Hologic's ThinPrep. We are interested to have some discussions with senior cytologists to better understand this duopoly situation. Is there room still for manual PAP testing in the US market? Is there room for new entrants? The areas we would like to discuss are: * Differences in technology - pros and cons of different systems * Full automation including imaging versus semi - automation? Productivity vs quality * Pricing of individual tests * Capital costs and consumables cost trends - leasing/reagent rental deal? Affordability for small labs? * Competition in this duopoly - is there any real competition? Basis? Positioning? * Cost of switching It would be a 45- 60 min telephone" Kind regards Charles Rowlands From bakevictoria at gmail.com Mon Jul 29 20:45:40 2019 From: bakevictoria at gmail.com (Victoria Baker) Date: Mon, 29 Jul 2019 21:45:40 -0400 Subject: [Histonet] CD27 Message-ID: Hi! Is anyone currently using CD27 on FFPE human tissue? I found one source and it is for lyophilized CD27. I'd like to find one that is a concentrate. I have given up on a pre-dilute. I would like to see if there is both a mono and a poly clonal ab available and is I'm use. Thank you in advance for your help. Vikki Thank From michael.gudo at morphisto.de Tue Jul 30 06:01:02 2019 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Tue, 30 Jul 2019 13:01:02 +0200 Subject: [Histonet] Victoria blue for lung tissue In-Reply-To: <1564264394036.68685@health.nsw.gov.au> References: <1564188227985.76571@health.nsw.gov.au> <1564264394036.68685@health.nsw.gov.au> Message-ID: <122E361B-D386-4AF6-A8CC-DDB4377A3BE8@morphisto.de> There is also a Kit and the ready to use solution available from MORPHISTO Germany. Just contact the sales office: info at morphisto.de Cheers Michael > Am 27.07.2019 um 23:53 schrieb Tony Henwood (SCHN) via Histonet : > > Methods found here: > > https://www.ihcworld.com/_protocols/special_stains/miller's_elastic_ellis.htm > > http://www.histosearch.com/histonet/Dec98/Millersstainforelasticfib.html > > https://www.sakuraus.com/getattachment/774d89be-4b32-4d69-bb02-8dea75c52c0c/858 > > ? > > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > ________________________________ > From: Bob Richmond > Sent: Saturday, July 27, 2019 11:27:54 AM > To: Tony Henwood (SCHN) > Subject: Re: [Histonet] Victoria blue for lung tissue > > Is this method published anywhere that "Amy" would likely have access to? This looked to me like one of those cases of the blind leading the blind that are all to common in research involving histochemistry. > > On Fri, Jul 26, 2019 at 8:44 PM Tony Henwood (SCHN) > wrote: > Victoria Blue is the dye used in Miller's Stain for Elastic Tissue. > It is also used in the Roche Ventana Benchmark Stainer to stain elastic Tissue: > > Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148-149 > > Karen Percival & Zaher Radi (2017) Comparison of five elastin histochemical stains to identify pulmonary small vasculature, Journal of Histotechnology, 40:3, 73-78 > > Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in Human Skin" Histologic 32(1): 12 > > Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 1996: 23: 37-42. > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > ________________________________________ > From: Bob Richmond via Histonet > > Sent: Saturday, July 27, 2019 4:10 AM > To: Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Victoria blue for lung tissue > > Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE > lung tissue to exam thickness of artery. Could anybody recommend a good > vendor of this reagent kit?<< > > You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and > several others. I couldn't find anyone who offers a kit, only the dry dye. > There are other dyes called Victoria Blue, reported to give the same > results. > > You'd have to find a method for preparing it as an elastic stain, and I > couldn't find such a method either with Google or in my old books. The > requester needs to supply you with a method. I suspect the requester is > reading an old article. There are stains for elastic tissue (which is I > suppose what you want to "exam thickness of artery") that are a lot easier > to get. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. > > > This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************** MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From relia1 at earthlink.net Tue Jul 30 11:48:03 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 30 Jul 2019 12:48:03 -0400 Subject: [Histonet] RELIA HOT JOB Alert!! New Opportunities in VA, CA and Message-ID: <001401d546f6$8e39cef0$aaad6cd0$@earthlink.net> Hi Histonetters! I hope you are having a great day. I wanted to give you a heads up on some exciting new opportunities I am working on. All of these are full time permanent day /early morning shift positions. Virginia: IHC Specialist Histotech ? days California: Early Morning Days shift positions in: San Diego Chico San Francisco Suburbs NY/NJ Greater NYC Metro area Histology Sales Manager IHC Specialist All of these opportunities are full time and permanent and my clients offer relocation assistance, amazing benefits and a great place to work. Some of them are offering sign on bonuses and some are RELIA exclusives. For more info ASAP call or text me at 407-353-5070 or shoot me an email at relia1 at earthlink.net Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From kmilne at bccrc.ca Tue Jul 30 12:19:54 2019 From: kmilne at bccrc.ca (Katy Milne) Date: Tue, 30 Jul 2019 17:19:54 +0000 Subject: [Histonet] CD27 In-Reply-To: References: Message-ID: We use this RbMAb from Abcam... https://www.abcam.com/cd27-antibody-epr8569-ab131254.html -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: July-30-19 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 188, Issue 23 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. CD27 (Victoria Baker) 2. Re: Victoria blue for lung tissue (Dr. Michael Gudo (Morphisto GmbH)) ---------------------------------------------------------------------- Message: 1 Date: Mon, 29 Jul 2019 21:45:40 -0400 From: Victoria Baker To: Histo Net list server Subject: [Histonet] CD27 Message-ID: Content-Type: text/plain; charset="UTF-8" Hi! Is anyone currently using CD27 on FFPE human tissue? I found one source and it is for lyophilized CD27. I'd like to find one that is a concentrate. I have given up on a pre-dilute. I would like to see if there is both a mono and a poly clonal ab available and is I'm use. Thank you in advance for your help. Vikki Thank ------------------------------ Message: 2 Date: Tue, 30 Jul 2019 13:01:02 +0200 From: "Dr. Michael Gudo (Morphisto GmbH)" To: "Tony Henwood (SCHN)" Cc: Bob Richmond , Histonet Subject: Re: [Histonet] Victoria blue for lung tissue Message-ID: <122E361B-D386-4AF6-A8CC-DDB4377A3BE8 at morphisto.de> Content-Type: text/plain; charset=utf-8 There is also a Kit and the ready to use solution available from MORPHISTO Germany. Just contact the sales office: info at morphisto.de Cheers Michael > Am 27.07.2019 um 23:53 schrieb Tony Henwood (SCHN) via Histonet : > > Methods found here: > > https://www.ihcworld.com/_protocols/special_stains/miller's_elastic_el > lis.htm > > http://www.histosearch.com/histonet/Dec98/Millersstainforelasticfib.ht > ml > > https://www.sakuraus.com/getattachment/774d89be-4b32-4d69-bb02-8dea75c > 52c0c/858 > > ? > > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children's Hospital at Westmead Adjunct > Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, > Westmead NSW 2145, AUSTRALIA ________________________________ > From: Bob Richmond > Sent: Saturday, July 27, 2019 11:27:54 AM > To: Tony Henwood (SCHN) > Subject: Re: [Histonet] Victoria blue for lung tissue > > Is this method published anywhere that "Amy" would likely have access to? This looked to me like one of those cases of the blind leading the blind that are all to common in research involving histochemistry. > > On Fri, Jul 26, 2019 at 8:44 PM Tony Henwood (SCHN) > wrote: > Victoria Blue is the dye used in Miller's Stain for Elastic Tissue. > It is also used in the Roche Ventana Benchmark Stainer to stain elastic Tissue: > > Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148-149 > > Karen Percival & Zaher Radi (2017) Comparison of five elastin > histochemical stains to identify pulmonary small vasculature, Journal > of Histotechnology, 40:3, 73-78 > > Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in > Human Skin" Histologic 32(1): 12 > > Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 1996: 23: 37-42. > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children's Hospital at Westmead Adjunct > Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, > Westmead NSW 2145, AUSTRALIA > > ________________________________________ > From: Bob Richmond via Histonet > n.edu>> > Sent: Saturday, July 27, 2019 4:10 AM > To: > Histonet at lists.utsouthwestern.edu .edu> > Subject: Re: [Histonet] Victoria blue for lung tissue > > Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent > FFPE lung tissue to exam thickness of artery. Could anybody recommend > a good vendor of this reagent kit?<< > > You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich > and several others. I couldn't find anyone who offers a kit, only the dry dye. > There are other dyes called Victoria Blue, reported to give the same > results. > > You'd have to find a method for preparing it as an elastic stain, and > I couldn't find such a method either with Google or in my old books. > The requester needs to supply you with a method. I suspect the > requester is reading an old article. There are stains for elastic > tissue (which is I suppose what you want to "exam thickness of > artery") that are a lot easier to get. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu .edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. > > > This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************** MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 188, Issue 23 ***************************************** From Nancy_Schmitt at pa-ucl.com Tue Jul 30 14:11:02 2019 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Tue, 30 Jul 2019 19:11:02 +0000 Subject: [Histonet] General Data Laser Track PH6 Message-ID: Hello- Is anyone using cassettes from a different manufacturer than the ones supplied with General Data? We are looking for an alternative mesh cassette that works with the engraver since theirs does not utilize the whole inside of the cassette - there is an inner square space where the tissue is placed and a moat that surrounds it (for lack of a better explanation). With appreciation, Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager From Nancy_Schmitt at pa-ucl.com Tue Jul 30 14:13:55 2019 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Tue, 30 Jul 2019 19:13:55 +0000 Subject: [Histonet] BOND Max from Leica Message-ID: <1d75259b094440d8a9f22d5f8e48f572@pa-ucl.com> Hello- Looking for people that have installed a BOND Max from Leica in the past year, are using for general Anatomic Path IHC use, and are willing to have a chat and offer a few pointers. Thanks, Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager United Clinical Laboratories Dubuque, IA 52001 From ASelf at tidelandshealth.org Wed Jul 31 08:26:54 2019 From: ASelf at tidelandshealth.org (Amy Self) Date: Wed, 31 Jul 2019 13:26:54 +0000 Subject: [Histonet] Dedicated Pathology Information Systems Message-ID: Good Morning All, How many of you out there in histoland have a dedicated pathology information software system that can integrate with voice recognition? If you would please tell me the pros and cons of what you have in place and if it can integrate with Meditech. We currently utilize Meditech and Voicebrook in our facility. Thanks in advance for your help, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 (843) 520-8711 ASelf at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From criley at dpspa.com Wed Jul 31 13:28:21 2019 From: criley at dpspa.com (Charles Riley) Date: Wed, 31 Jul 2019 14:28:21 -0400 Subject: [Histonet] Salivary Amylase by IHC Message-ID: Does anyone run salivary amylase testing via IHC. I use the bond platforms and am looking for an antibody that is not Research only based -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs