From john.garratt at ciqc.ca Tue Jan 1 16:13:17 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Tue, 01 Jan 2019 22:13:17 +0000 Subject: [Histonet] Multi-Tumor Controls for IHC Validation In-Reply-To: References: Message-ID: For a basic multi-tissue control I recommend tonsil, liver, appendix and pancreas. Ensure you standardize fixation and processing time for the tissues you use I suggest that labs use iCAPCs (immunohistochemistry critical assay performance controls) for developing your controls. This article (Standardization of Positive Controls in Immunohistochemistry) and will explain iCAPCs. https://www.researchgate.net/publication/282488454_Standardization_of_Positive_Controls_in_Diagnostic_Immunohistochemistry All the best for 2019 John www.ciqc.ca ??????? Original Message ??????? On Monday, December 31, 2018 10:39 AM, Miranda Giorgi via Histonet wrote: > Hello, > > Does anyone use multi tumor tissue controls for their IHC validations? If so, what do you use in them? > > We have been staining multi-tumor controls with our validation sets on top of the 10 positive and 10 negative cases to test specificity, but we are wondering if this is necessary. Plus, the blocks are sometimes difficult to maintain. I'd love to hear what other labs are doing. > > Thank you, > > Miranda Giorgi, HTL (ASCP)cm > Histology Manager > Incyte Diagnostics > 509-892-2744 > > This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700. > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From caymanfleck at hotmail.com Wed Jan 2 20:48:10 2019 From: caymanfleck at hotmail.com (Cayman Fleck) Date: Thu, 3 Jan 2019 02:48:10 +0000 Subject: [Histonet] IHC Negative reagent controls Message-ID: A question that came up regarding negative reagent controls for IHC...currently using Ventana i-View. Our regular negative control goes through the standard antigen retrieval steps, like 99% of our antibodies. However there are a small number of antibodies that require enzyme as well (Protease 1). I've seen a number of suggestions regarding this for the negative reagent control...some say use an additional negative control protocol that includes the protease, some say to use a single negative control protocol and just include the harshest cell conditioning that any of your protocols use (so basically use the cell conditioning + protease negative control for all antibodies)...i-View is not polymer-based so we need to continue using negative controls. Any thoughts or advice? Frank Sent from Outlook From tony.henwood at health.nsw.gov.au Wed Jan 2 22:06:57 2019 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 3 Jan 2019 04:06:57 +0000 Subject: [Histonet] IHC Negative reagent controls In-Reply-To: References: Message-ID: <0e5c3ac509b7424e942160149bb36a4b@SVDCMBX-MEX024.nswhealth.net> Hi Cayman, Unfortunately, applying HIER to a negative control for an antibody that requires enzyme retrieval (or no retrieval at all) is not appropriate. The pre-treatment processes are different and could unmask different epitopes. If you are using a negative control then the whole procedure needs to be same with the exception or replacing the localisation antibody with an Isotypic antibody solution. (Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application.) For example, applying citrate or EDTA HIER to sections prior to using the CD99 antibody (clone 12E7) can reveal perinuclear (golgi-like) staining of some tumours (eg some colonic carcinomas) but this is not seen if enzyme retrieval is used. Hope this is useful Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Cayman Fleck via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 3 January 2019 1:48 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC Negative reagent controls A question that came up regarding negative reagent controls for IHC...currently using Ventana i-View. Our regular negative control goes through the standard antigen retrieval steps, like 99% of our antibodies. However there are a small number of antibodies that require enzyme as well (Protease 1). I've seen a number of suggestions regarding this for the negative reagent control...some say use an additional negative control protocol that includes the protease, some say to use a single negative control protocol and just include the harshest cell conditioning that any of your protocols use (so basically use the cell conditioning + protease negative control for all antibodies)...i-View is not polymer-based so we need to continue using negative controls. Any thoughts or advice? Frank Sent from Outlook _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From hmarlatt26 at gmail.com Thu Jan 3 09:19:15 2019 From: hmarlatt26 at gmail.com (Heather Marlatt) Date: Thu, 3 Jan 2019 08:19:15 -0700 Subject: [Histonet] AM to statlab and recommendations Message-ID: Did anyone else use American Mastertech and is now super frustrated with Stat Lab??? I?ve used AM for years and loved them, great prices and fast shipping, now cost is through the roof and they?re shipping all my stuff separately costing a fortune! Looking for recommendations on xylene alternatives and safe mounting medium. I?ve been using transcend and cover safe two American Mastertech signature products but I?m running for my life. I?ll be contacting a few of the other vendors I use as well but I?d like some recommendations on xylene alternatives we?ve been xylene free for 4 years and I?m not going back. Thank you Heather From john.garratt at ciqc.ca Thu Jan 3 10:41:03 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 03 Jan 2019 16:41:03 +0000 Subject: [Histonet] IHC Negative reagent controls In-Reply-To: References: Message-ID: The article referenced below may be of interest. Standardization of Negative Controls in Diagnostic Immunohistochemistry: Recommendations From the International Ad Hoc Expert Panel https://www.researchgate.net/publication/261516067_Standardization_of_Negative_Controls_in_Diagnostic_Immunohistochemistry_Recommendations_From_the_International_Ad_Hoc_Expert_Panel John www.ciqc.ca ??????? Original Message ??????? On Wednesday, January 2, 2019 6:48 PM, Cayman Fleck via Histonet wrote: > A question that came up regarding negative reagent controls for IHC...currently using Ventana i-View. Our regular negative control goes through the standard antigen retrieval steps, like 99% of our antibodies. However there are a small number of antibodies that require enzyme as well (Protease 1). I've seen a number of suggestions regarding this for the negative reagent control...some say use an additional negative control protocol that includes the protease, some say to use a single negative control protocol and just include the harshest cell conditioning that any of your protocols use (so basically use the cell conditioning + protease negative control for all antibodies)...i-View is not polymer-based so we need to continue using negative controls. Any thoughts or advice? > > Frank > > Sent from Outlookhttp://aka.ms/weboutlook > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague at Pathologyarts.com Thu Jan 3 11:05:21 2019 From: c.tague at Pathologyarts.com (Curt) Date: Thu, 3 Jan 2019 17:05:21 +0000 Subject: [Histonet] IHC-H&E-IHC HELP... Message-ID: <9C8F910F72893643B3C3793C3D67132B94D03BD4@PATHOLOGYSERVER.pathologyarts.local> Need help before I turn a mistake into an irreparable mistake... We have some unstained slides that were supposed to get stained with H&E but my guy stained them with IHC. It's complicated, we received slides and a block, the block was for IHC, the unstained slides were for H&E, he inverted the process) The point is, now the unstained slides are stained with IHC... I know we cannot destain the IHC but we can simply run and H&E over them... the real question I have is subsequent to the H&E... this pathologist generally likes to see the H&E then order IHC on them based on what he sees (we only have these few unstained slides, don't have blocks to recut)... So the question is... if we've already run IHC, then followed that with and H&E, can we return to run IHC on the slides again? would you want to skip any pre-treatment, antigen retrieval???? I don't see this working too well myself, if they're already stained with DAB, that would be present on the second stain... Thoughts? Thanks for your help. Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. From john at imebinc.com Thu Jan 3 12:56:32 2019 From: john at imebinc.com (John O'Brien) Date: Thu, 3 Jan 2019 10:56:32 -0800 Subject: [Histonet] Vendor with all items needed/ Histonet Digest, Vol 182, Issue 3 Message-ID: <030201d4a396$0b5ec890$221c59b0$@imebinc.com> Hi Heather, Please contact IMEB INC for pricing and service on all your pathology supplies, chemical needs and Pathology instruments. We will provide the service you require to keep cost down, Contact travis at imebinc.com Or sales at imebinc.com Web site www.imebinc.com Regards, IMEB INC -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, January 03, 2019 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 182, Issue 3 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. IHC Negative reagent controls (Cayman Fleck) 2. Re: IHC Negative reagent controls (Tony Henwood (SCHN)) 3. AM to statlab and recommendations (Heather Marlatt) 4. Re: IHC Negative reagent controls (John Garratt) 5. IHC-H&E-IHC HELP... (Curt) ---------------------------------------------------------------------- Message: 1 Date: Thu, 3 Jan 2019 02:48:10 +0000 From: Cayman Fleck To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] IHC Negative reagent controls Message-ID: Content-Type: text/plain; charset="iso-8859-1" A question that came up regarding negative reagent controls for IHC...currently using Ventana i-View. Our regular negative control goes through the standard antigen retrieval steps, like 99% of our antibodies. However there are a small number of antibodies that require enzyme as well (Protease 1). I've seen a number of suggestions regarding this for the negative reagent control...some say use an additional negative control protocol that includes the protease, some say to use a single negative control protocol and just include the harshest cell conditioning that any of your protocols use (so basically use the cell conditioning + protease negative control for all antibodies)...i-View is not polymer-based so we need to continue using negative controls. Any thoughts or advice? Frank Sent from Outlook ------------------------------ Message: 2 Date: Thu, 3 Jan 2019 04:06:57 +0000 From: "Tony Henwood (SCHN)" To: Cayman Fleck Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] IHC Negative reagent controls Message-ID: <0e5c3ac509b7424e942160149bb36a4b at SVDCMBX-MEX024.nswhealth.net> Content-Type: text/plain; charset="us-ascii" Hi Cayman, Unfortunately, applying HIER to a negative control for an antibody that requires enzyme retrieval (or no retrieval at all) is not appropriate. The pre-treatment processes are different and could unmask different epitopes. If you are using a negative control then the whole procedure needs to be same with the exception or replacing the localisation antibody with an Isotypic antibody solution. (Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application.) For example, applying citrate or EDTA HIER to sections prior to using the CD99 antibody (clone 12E7) can reveal perinuclear (golgi-like) staining of some tumours (eg some colonic carcinomas) but this is not seen if enzyme retrieval is used. Hope this is useful Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Cayman Fleck via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 3 January 2019 1:48 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC Negative reagent controls A question that came up regarding negative reagent controls for IHC...currently using Ventana i-View. Our regular negative control goes through the standard antigen retrieval steps, like 99% of our antibodies. However there are a small number of antibodies that require enzyme as well (Protease 1). I've seen a number of suggestions regarding this for the negative reagent control...some say use an additional negative control protocol that includes the protease, some say to use a single negative control protocol and just include the harshest cell conditioning that any of your protocols use (so basically use the cell conditioning + protease negative control for all antibodies)...i-View is not polymer-based so we need to continue using negative controls. Any thoughts or advice? Frank Sent from Outlook _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ------------------------------ Message: 3 Date: Thu, 3 Jan 2019 08:19:15 -0700 From: Heather Marlatt To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] AM to statlab and recommendations Message-ID: Content-Type: text/plain; charset="UTF-8" Did anyone else use American Mastertech and is now super frustrated with Stat Lab??? I?ve used AM for years and loved them, great prices and fast shipping, now cost is through the roof and they?re shipping all my stuff separately costing a fortune! Looking for recommendations on xylene alternatives and safe mounting medium. I?ve been using transcend and cover safe two American Mastertech signature products but I?m running for my life. I?ll be contacting a few of the other vendors I use as well but I?d like some recommendations on xylene alternatives we?ve been xylene free for 4 years and I?m not going back. Thank you Heather ------------------------------ Message: 4 Date: Thu, 03 Jan 2019 16:41:03 +0000 From: John Garratt To: Cayman Fleck Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] IHC Negative reagent controls Message-ID: Content-Type: text/plain; charset=UTF-8 The article referenced below may be of interest. Standardization of Negative Controls in Diagnostic Immunohistochemistry: Recommendations From the International Ad Hoc Expert Panel https://www.researchgate.net/publication/261516067_Standardization_of_Negati ve_Controls_in_Diagnostic_Immunohistochemistry_Recommendations_From_the_Inte rnational_Ad_Hoc_Expert_Panel John www.ciqc.ca ??????? Original Message ??????? On Wednesday, January 2, 2019 6:48 PM, Cayman Fleck via Histonet wrote: > A question that came up regarding negative reagent controls for IHC...currently using Ventana i-View. Our regular negative control goes through the standard antigen retrieval steps, like 99% of our antibodies. However there are a small number of antibodies that require enzyme as well (Protease 1). I've seen a number of suggestions regarding this for the negative reagent control...some say use an additional negative control protocol that includes the protease, some say to use a single negative control protocol and just include the harshest cell conditioning that any of your protocols use (so basically use the cell conditioning + protease negative control for all antibodies)...i-View is not polymer-based so we need to continue using negative controls. Any thoughts or advice? > > Frank > > Sent from Outlookhttp://aka.ms/weboutlook > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 3 Jan 2019 17:05:21 +0000 From: Curt To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] IHC-H&E-IHC HELP... Message-ID: <9C8F910F72893643B3C3793C3D67132B94D03BD4 at PATHOLOGYSERVER.pathologyarts.loca l> Content-Type: text/plain; charset="us-ascii" Need help before I turn a mistake into an irreparable mistake... We have some unstained slides that were supposed to get stained with H&E but my guy stained them with IHC. It's complicated, we received slides and a block, the block was for IHC, the unstained slides were for H&E, he inverted the process) The point is, now the unstained slides are stained with IHC... I know we cannot destain the IHC but we can simply run and H&E over them... the real question I have is subsequent to the H&E... this pathologist generally likes to see the H&E then order IHC on them based on what he sees (we only have these few unstained slides, don't have blocks to recut)... So the question is... if we've already run IHC, then followed that with and H&E, can we return to run IHC on the slides again? would you want to skip any pre-treatment, antigen retrieval???? I don't see this working too well myself, if they're already stained with DAB, that would be present on the second stain... Thoughts? Thanks for your help. Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 182, Issue 3 **************************************** From Shannon.Logan at bellin.org Thu Jan 3 14:03:30 2019 From: Shannon.Logan at bellin.org (Logan, Shannon) Date: Thu, 3 Jan 2019 20:03:30 +0000 Subject: [Histonet] Xylene substitute Message-ID: <5b53c35d57274f30b5ee9624f0d56cad@BAPWEXCH001b.bellin.com> Hi Heather, We have been using Formula 83 by CBG Biotech for over 7 years now with no problems, we even recycle it. Shannon H. Logan B.S., HTL (ASCP) Pathology Department Bellin Health Memorial Hospital 744 South Webster Avenue Green Bay, WI 54305-3400 920-433-3653 X3727 From tony.henwood at health.nsw.gov.au Thu Jan 3 17:27:21 2019 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 3 Jan 2019 23:27:21 +0000 Subject: [Histonet] IHC-H&E-IHC HELP... In-Reply-To: <9C8F910F72893643B3C3793C3D67132B94D03BD4@PATHOLOGYSERVER.pathologyarts.local> References: <9C8F910F72893643B3C3793C3D67132B94D03BD4@PATHOLOGYSERVER.pathologyarts.local> Message-ID: Yep, Definitely an issue. You can easily stain the IPX slides with H&E, though the discernibility of the cells and tissue structure with the H&E will depend on the degree of DAB product laid down (eg I would expect it to be difficult with a Vimentin IPX compared to a CD15). (Grosset, A. A., Loayza-Vega, K., Adam-Granger, ?., Birlea, M., Gilks, B., Nguyen, B., ... & Trudel, D. (2017). Hematoxylin and Eosin Counterstaining Protocol for Immunohistochemistry Interpretation and Diagnosis. Applied immunohistochemistry & molecular morphology: AIMM.) As for doing another IPX on the existing IPX stained section (with DAB as the chromogen), you will have to use a different label (eg alkaline phosphatase). The result will depend on the cell compartment the two antigens exist. If the DAB is nuclear, then a cytoplasm or cytoplasmic membrane localisation with the Alk Phosphatase will work. If both antigens are cytoplasmic, then you will not see co-localisation in the same cell since the DAB will prevent any antibody binding in the same compartment. Assuming the above is good, then since the antigen retrieval tends to reverse over time, I would include a short retrieval before the second antibody. Now after all that, I hope the section stays on the slide! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Curt via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 4 January 2019 4:05 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC-H&E-IHC HELP... Need help before I turn a mistake into an irreparable mistake... We have some unstained slides that were supposed to get stained with H&E but my guy stained them with IHC. It's complicated, we received slides and a block, the block was for IHC, the unstained slides were for H&E, he inverted the process) The point is, now the unstained slides are stained with IHC... I know we cannot destain the IHC but we can simply run and H&E over them... the real question I have is subsequent to the H&E... this pathologist generally likes to see the H&E then order IHC on them based on what he sees (we only have these few unstained slides, don't have blocks to recut)... So the question is... if we've already run IHC, then followed that with and H&E, can we return to run IHC on the slides again? would you want to skip any pre-treatment, antigen retrieval???? I don't see this working too well myself, if they're already stained with DAB, that would be present on the second stain... Thoughts? Thanks for your help. Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From mjdessoye at commonwealthhealth.net Fri Jan 4 07:47:57 2019 From: mjdessoye at commonwealthhealth.net (Dessoye, Michael) Date: Fri, 4 Jan 2019 13:47:57 +0000 Subject: [Histonet] Specimen disposal bags Message-ID: Hello Histonet, I'm looking for some recommendations on biohazard bags for specimen disposal. Currently using Brighton 3.0 mil 44 gallon bags and they have occasional problems with leakage. Obviously the formalin is removed but there is always some residual formalin present. I'm willing to pay a little more for a better quality bag that is more leak-resistant. Any advice? Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | Commonwealth Health Laboratory Services | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 ********************************************************************** Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From greg.dobbin at gmail.com Fri Jan 4 09:40:40 2019 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Fri, 4 Jan 2019 11:40:40 -0400 Subject: [Histonet] IHC-H&E-IHC HELP... Message-ID: Hi Curt, You can de-stain the hematoxylin counter stain and then do H&E and all should be fine. Subsequent IHC is possible but obviously you would need a different color chromogen to differentiate the new stain from the previous one. There may be a problem getting the section stay on through a second IHC procedure but maybe not. I would stain a control section for the desired Ab using the retrieval method that was used initially and see how the control looks. If the pathologist thinks the that this control is adequate for interpretation, then restain the slides with the desired Ab and no additional retrieval. Cheers, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From hmarlatt26 at gmail.com Fri Jan 4 13:44:19 2019 From: hmarlatt26 at gmail.com (Heather Marlatt) Date: Fri, 4 Jan 2019 12:44:19 -0700 Subject: [Histonet] Thank you for input Message-ID: Thank you to everyone for the recommendations for xylene alternatives. Stat lab is trying to make it right and I have a lot of options to explore now. Thanks Heather From lmarie08 at uga.edu Fri Jan 4 13:57:41 2019 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Fri, 4 Jan 2019 19:57:41 +0000 Subject: [Histonet] lab manager position open UGA Message-ID: Good Afternoon, I reached out a couple of months ago about the Histology Laboratory Manager position coming open at the University of Georgia. After a small delay with a system change-over, the position is posted! If you are still interested please follow the link below to officially apply. Good luck! https://www.ugajobsearch.com/postings/59337 Lauren Lauren Sweeney Laboratory Manager Histology University of Georgia | College of Veterinary Medicine Department of Population Health Poultry Diagnostic Research Center 953 College Station Rd | Athens, GA 30605 706-583-0636 | Lmarie08 at uga.edu From lmarie08 at uga.edu Fri Jan 4 14:01:13 2019 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Fri, 4 Jan 2019 20:01:13 +0000 Subject: [Histonet] link correction Message-ID: Apologies Histonetters, The working link is: https://www.ugajobsearch.com/postings/59337 for the UGA lab manager position. From ASelf at tidelandshealth.org Fri Jan 4 14:42:29 2019 From: ASelf at tidelandshealth.org (Amy Self) Date: Fri, 4 Jan 2019 20:42:29 +0000 Subject: [Histonet] Joint Commission Inspection Process Message-ID: Happy Friday and yaaaay it's almost time clock time, We have recently signed up to start getting inspected by Joint Commission. Does anyone have any resources that they could point me to? Does JC have a checklist like CAP does? Any information would be appreciated. Thanks in advance and I hope everyone has a fantabulous weekend. Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 (843) 520-8711 ASelf at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From Linda.Margraf at cookchildrens.org Mon Jan 7 11:54:22 2019 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Mon, 7 Jan 2019 17:54:22 +0000 Subject: [Histonet] FW: Pulmo Panel (+) Squamous Cell Lung ctrl In-Reply-To: <211EE529-79B9-4C20-913B-492F9EF77B56@gmail.com> References: <211EE529-79B9-4C20-913B-492F9EF77B56@gmail.com> Message-ID: <7d1b48f4f2054912aa056fadce18faa2@MBX10.CCHCS.LDAP> Here is a message I am posting for Chrissy?.. Begin forwarded message: From: "Altemus, Christine" < > Date: January 7, 2019 at 11:29:23 AM CST To: "lindamargraf at gmail.com" > Subject: Pulmo Panel (+) Squamous Cell Lung ctrl Hell o - Can you please post to Histonet website for me? I am looking for (+) Squamous cell lung cancer control for the Pulmo Panel IHC Stains. Thank you! Chrissy St. Mary's Healthcare Amsterdam, NY Christine.Altemus at ascension.org From john.garratt at ciqc.ca Mon Jan 7 14:19:24 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Mon, 07 Jan 2019 20:19:24 +0000 Subject: [Histonet] FW: Pulmo Panel (+) Squamous Cell Lung ctrl In-Reply-To: <7d1b48f4f2054912aa056fadce18faa2@MBX10.CCHCS.LDAP> References: <211EE529-79B9-4C20-913B-492F9EF77B56@gmail.com> <7d1b48f4f2054912aa056fadce18faa2@MBX10.CCHCS.LDAP> Message-ID: The important thing is knowing which markers you are using, or wish to use. Are you looking at using diagnostics IHC markers or prognostic markers in your panel, or both? John www.ciqc.ca ??????? Original Message ??????? On Monday, January 7, 2019 9:54 AM, Linda Margraf via Histonet wrote: > Here is a message I am posting for Chrissy?.. > Begin forwarded message: > From: "Altemus, Christine" < > > Date: January 7, 2019 at 11:29:23 AM CST > To: "lindamargraf at gmail.commailto:lindamargraf at gmail.com" > Subject: Pulmo Panel (+) Squamous Cell Lung ctrl > > Hell o - > > Can you please post to Histonet website for me? > > I am looking for (+) Squamous cell lung cancer control for the Pulmo Panel IHC Stains. Thank you! > > Chrissy > > St. Mary's Healthcare > > Amsterdam, NY > > Christine.Altemus at ascension.orgmailto:Christine.Altemus at ascension.org > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katherine at ka-recruiting.com Mon Jan 7 14:52:30 2019 From: katherine at ka-recruiting.com (Katherine Marano) Date: Mon, 7 Jan 2019 15:52:30 -0500 Subject: [Histonet] histotech needed in oregon Message-ID: Hi Histonetters, Happy New Year! I hope you enjoyed the holidays. I wanted to send you a quick email about a great new Histology position I am looking to fill in Northwest Oregon. This top facility is seeking a permanent and full-time Histotech to work 9a-5:30p. They are seeking someone with experience in QIHC or IHC. I would love to tell you more if you or anyone you know may be interested? They are offering competitive compensation & complete benefits and looking to hire asap. Send me a resume if you want to discuss all the details! I work on permanent positions nationwide so let me know if you may be looking for something different. Some of my other openings include: Atlanta, GA ? Histotech (7p-3:30 am) Central GA ? Histotech (day shift) Northwest IL ? Histotech (6a-2:30pm) Baltimore, MD ? Histotech (day shift) High Point, NC ? Histotech (1 days and 1 nights) Westchester County, NY ? Histotech (1 Tu-Sat days & 1 IHC 3p-11:30pm) Syracuse, NY ? Histotech (days) Eastern OH ? Histotech (M-F days) Southeast Tennessee ? Histotech (rotating schedule: M-F 10p-6a and 11p-7:30a) Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From Jessica.Piche at wtbyhosp.org Tue Jan 8 07:05:06 2019 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Tue, 8 Jan 2019 13:05:06 +0000 Subject: [Histonet] HSV 1&2 Control slides Message-ID: Good Morning Everyone, I was wondering where everyone is getting control slides for HSV1&2 Antibody? I can't seem to find a lot of options. Thanks in advance! Jessica Piche, HT(ASCP) Waterbury Hospital From relia1 at earthlink.net Tue Jan 8 09:48:39 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 8 Jan 2019 10:48:39 -0500 Subject: [Histonet] Happy New Year Histopeeps!!!! Message-ID: <000501d4a769$a0b3f560$e21be020$@earthlink.net> Hi Histonetters! Happy New Year!!!! Here?s to a terrific 2019!!!!!!! How did you ring in the New Year? I hope you had fun ushering out 2018 and welcoming in 2019. I know I am looking forward to an exciting new year. I wanted to drop you this quick line to let you know that I have been chatting with clients and have some exciting new opportunities. Here is a list of my current open positions: Senior Histotech ?Norfolk, VA-Days RELO and Generous Sign on Bonus!! *Territory Sales Manager - Texas!* *Territory Sales Manager ? Ohio!* ? Dermpath Histotech ? Birmingham, AL ? Histotech ? San Diego, CA ? must be CLIA qualified to gross. ? Histotech ? Springfield, MA ? Histotech ? Tallahassee, FL ? Histotech ? Manitowoc, WI ? Histotech ? Fayetteville, NC ? Histotech ? Chattanooga, TN All of these are full time positions and my clients offer excellent compensation, benefits and in most cases relocation assistance and or a sign on bonus. For more information please contact me by e-mail: relia1 at earthlink.net or toll free: 866-607-3542 or you can reach me on my cell/text: 407-353-5070. Histonetters! If none of these positions interest you and you are on the hunt for a new position NOW please drop me an email and let me know what you are looking for. My phone is ringing off the hook and your next opportunity could be just a phone call away. When I get the call I want to know to call YOU! If you are set in your current position Congratulations! And thank you for taking the time to read my email. I have new positions coming in all of the time and you never know when something might interest you or a friend or coworker. The best part is if you refer someone and I place them you will earn a referral fee! So please stay tuned for my next email! Happy New Year to You & Yours!! I wish you a year of love, laughter, good health & prosperity. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From amurvosh at advancederm.net Tue Jan 8 10:11:24 2019 From: amurvosh at advancederm.net (Anne Murvosh) Date: Tue, 8 Jan 2019 16:11:24 +0000 Subject: [Histonet] Grossing large ellipse melanomas Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7BDA950@Exchange.Advancederm.net> We are starting to keep our melanomas in-house now that we have a better processor, however I could use some tips or tricks on cutting large fatty skin ellipse specimens. My problem I think, is too small a knife, the specimens are not cutting even and the fat is getting in the way. So any suggestions or links to grossing techniques would be appreciated. Also, any ideas on better cutting utensils for these. Thanks Anne Murvosh HT From KGoodkowsky at goodwin.edu Tue Jan 8 10:32:02 2019 From: KGoodkowsky at goodwin.edu (Kelli Goodkowsky) Date: Tue, 8 Jan 2019 16:32:02 +0000 Subject: [Histonet] Electronic Safety Data Sheets Message-ID: Good Afternoon, I am interested in software to manage our Safety Data Sheets. We are looking for a system that allows for anyone in the college to view an SDS. Any guidance and/or feedback from users would be most appreciated. Thank you. Kelli Kelli Goodkowsky, M.Ed., HT (ASCP) Program Director Histologic Science President, Faculty Senate Goodwin College Health and Natural Sciences 2 King Court East Hartford, CT 06118 kgoodkowsky at goodwin.edu V (860) 727-6917 F (860) 906-1801 From tbraud at holyredeemer.com Tue Jan 8 11:36:19 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 8 Jan 2019 17:36:19 +0000 Subject: [Histonet] looking for a specific block storage cabinet Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF9129F@HRHEX02-HOS.holyredeemer.local> Hi Histo Peeps - I'm trying to find the brand of paraffin block storage cabinets that have a little more height clearance than the traditional Tissue-Tek block storage cabinets. They were shown to me about 3-4 years ago, but I've misplaced the literature. I think they were blue? Vendors welcome to respond, but please, only if you have the product to which I'm referring. Thanks, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From c.tague at Pathologyarts.com Tue Jan 8 12:20:53 2019 From: c.tague at Pathologyarts.com (Curt) Date: Tue, 8 Jan 2019 18:20:53 +0000 Subject: [Histonet] ASR validation Message-ID: <9C8F910F72893643B3C3793C3D67132B94D054F8@PATHOLOGYSERVER.pathologyarts.local> Hello to all again! Thank you for all your help and advice over the years... now I have 1 more question... recommended protocol for validating an ASR antibody? As a private lab, I'm assuming we are required to notify all clients that it was validated internally and that it is not IVD but rather ASR??? Thanks again! Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. From rsrichmond at gmail.com Tue Jan 8 14:04:27 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Tue, 8 Jan 2019 15:04:27 -0500 Subject: [Histonet] Grossing large ellipse melanomas In-Reply-To: References: Message-ID: Anne Murvosh HT at Advanced Dermatology & Skin Surgery in Washington state asks: >>We are starting to keep our melanomas in-house now that we have a better processor, however I could use some tips or tricks on cutting large fatty skin ellipse specimens. My problem I think, is too small a knife, the specimens are not cutting even and the fat is getting in the way. So any suggestions or links to grossing techniques would be appreciated. Also, any ideas on better cutting utensils for these?<< First of all, you need to work closely with your dermatopathologist on this - you shouldn't be expected to get it right all by yourself. You need to be able to photograph these gross specimens, and insert the photograph in the report. Gross the specimen as soon as you can, and then fix the slices overnight for processing. Cut on a good cutting surface in good light with a steady hand. They make disposable long scalpel blades. These are expensive and a little hard to find, and I've only ever seen them on two pathology services. Here are two resources: Cellpath - which I've used - excellent knives https://www.fishersci.com/shop/products/macro-knife-8inch-10-pk/22222042 Sakura - haven't tried these https://www.sakuraus.com/Products/Grossing-Trimming/Accu-Edge-Blade-System/Trimming-Knife-Series.html Bob Richmond Samurai Pathologist Maryville TN From garethdavisyuma at gmail.com Wed Jan 9 16:14:48 2019 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Wed, 9 Jan 2019 15:14:48 -0700 Subject: [Histonet] Opinion on dye on biopsies Message-ID: So, I work in a small GI lab, and I put Eosin in my first formalin on my processor. My biopsies are very small and this helps, somewhat, to see the specimens for embedding and cutting. But, unfortunately, the esophagus tissues do not absorb the eosin much. Anyway, the hospital lab I work, part-time, in has started using hematoxylin to help see their biopsies. I happen to embed there and I think it just makes a big mess and the tissue does not absorb much of the stain. What are other labs doing to aid in making their small biopsies easier to see? What are pros and cons to doing this, in your opinion? Thanks! -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From tony.henwood at health.nsw.gov.au Wed Jan 9 17:09:29 2019 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 9 Jan 2019 23:09:29 +0000 Subject: [Histonet] Opinion on dye on biopsies In-Reply-To: References: Message-ID: <2605535c52f94da4be0d45da58196cff@SVDCMBX-MEX024.nswhealth.net> One article that might be useful is: Surprenant D, Garib G, Hutchens K, Dreifke M, Speiser J, Winterfield L, Peterson A, Krol C, Adams W, Tung R. Novel Use of Preoperative Epidermal Coloring of Very Small Dermatological Specimens-Protocol for Reduction of Lost Specimens. The American Journal of Dermatopathology. 2016 Jul 1;38(7):510-2. I would expect that this would colour the squamous cell layer of the oesophageal mucosa. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Gareth Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 10 January 2019 9:15 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Opinion on dye on biopsies So, I work in a small GI lab, and I put Eosin in my first formalin on my processor. My biopsies are very small and this helps, somewhat, to see the specimens for embedding and cutting. But, unfortunately, the esophagus tissues do not absorb the eosin much. Anyway, the hospital lab I work, part-time, in has started using hematoxylin to help see their biopsies. I happen to embed there and I think it just makes a big mess and the tissue does not absorb much of the stain. What are other labs doing to aid in making their small biopsies easier to see? What are pros and cons to doing this, in your opinion? Thanks! -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From ronald.kusters at pfmmedical.com Thu Jan 10 02:59:35 2019 From: ronald.kusters at pfmmedical.com (Kusters, Ronald) Date: Thu, 10 Jan 2019 08:59:35 +0000 Subject: [Histonet] Grossing large ellipse melanomas In-Reply-To: References: Message-ID: Look here as well: https://system.na3.netsuite.com/core/media/media.nl?id=2286981&c=3378782&h=aa4430177bb0a084b28e&_xt=.pdf&_ga=2.246879180.97703180.1547110574-143004537.1535466547 Ronald -----Original Message----- From: Bob Richmond [mailto:rsrichmond at gmail.com] Sent: 08 January 2019 21:04 To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Grossing large ellipse melanomas Anne Murvosh HT at Advanced Dermatology & Skin Surgery in Washington state asks: >>We are starting to keep our melanomas in-house now that we have a >>better processor, however I could use some tips or tricks on cutting large fatty skin ellipse specimens. My problem I think, is too small a knife, the specimens are not cutting even and the fat is getting in the way. So any suggestions or links to grossing techniques would be appreciated. Also, any ideas on better cutting utensils for these?<< First of all, you need to work closely with your dermatopathologist on this - you shouldn't be expected to get it right all by yourself. You need to be able to photograph these gross specimens, and insert the photograph in the report. Gross the specimen as soon as you can, and then fix the slices overnight for processing. Cut on a good cutting surface in good light with a steady hand. They make disposable long scalpel blades. These are expensive and a little hard to find, and I've only ever seen them on two pathology services. Here are two resources: Cellpath - which I've used - excellent knives https://www.fishersci.com/shop/products/macro-knife-8inch-10-pk/22222042 Sakura - haven't tried these https://www.sakuraus.com/Products/Grossing-Trimming/Accu-Edge-Blade-System/Trimming-Knife-Series.html Bob Richmond Samurai Pathologist Maryville TN From CDavis at che-east.org Thu Jan 10 13:24:32 2019 From: CDavis at che-east.org (Cassie P. Davis) Date: Thu, 10 Jan 2019 19:24:32 +0000 Subject: [Histonet] Eosin on processor for biopsies In-Reply-To: References: Message-ID: <0e6f1a27ce4c49ca924312807575360c@che-east.org> Gareth, reguarding the eosin biopsy post on the histo net. Things you might want to considered looking at, I cannot say for sure because I don't know your processing solutions/schedule: Most Eosins are alcohol soluable, by putting the eosin in your formalin most of it will wash out during processing. Many labs put the Eosin in the last alcohol. Hematoxylin is typically water soluable, if you put it in formailin processing usually removes water if the formailin is followed by alcohols. I hope this helps, Cassie From: Gareth Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 10 January 2019 9:15 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Opinion on dye on biopsies So, I work in a small GI lab, and I put Eosin in my first formalin on my processor. My biopsies are very small and this helps, somewhat, to see the specimens for embedding and cutting. But, unfortunately, the esophagus tissues do not absorb the eosin much. Anyway, the hospital lab I work, part-time, in has started using hematoxylin to help see their biopsies. I happen to embed there and I think it just makes a big mess and the tissue does not absorb much of the stain. What are other labs doing to aid in making their small biopsies easier to see? What are pros and cons to doing this, in your opinion? Thanks! -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From lblazek at digestivespecialists.com Thu Jan 10 14:05:57 2019 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Thu, 10 Jan 2019 20:05:57 +0000 Subject: [Histonet] Eosin on processor for biopsies In-Reply-To: <0e6f1a27ce4c49ca924312807575360c@che-east.org> References: <0e6f1a27ce4c49ca924312807575360c@che-east.org> Message-ID: We have put eosin in the last alcohol on the processor for years. It stays in the esophageal biopsies just fine. Linda -----Original Message----- From: Cassie P. Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, January 10, 2019 2:25 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Eosin on processor for biopsies Gareth, reguarding the eosin biopsy post on the histo net. Things you might want to considered looking at, I cannot say for sure because I don't know your processing solutions/schedule: Most Eosins are alcohol soluable, by putting the eosin in your formalin most of it will wash out during processing. Many labs put the Eosin in the last alcohol. Hematoxylin is typically water soluable, if you put it in formailin processing usually removes water if the formailin is followed by alcohols. I hope this helps, Cassie From: Gareth Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 10 January 2019 9:15 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Opinion on dye on biopsies So, I work in a small GI lab, and I put Eosin in my first formalin on my processor. My biopsies are very small and this helps, somewhat, to see the specimens for embedding and cutting. But, unfortunately, the esophagus tissues do not absorb the eosin much. Anyway, the hospital lab I work, part-time, in has started using hematoxylin to help see their biopsies. I happen to embed there and I think it just makes a big mess and the tissue does not absorb much of the stain. What are other labs doing to aid in making their small biopsies easier to see? What are pros and cons to doing this, in your opinion? Thanks! -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Will.Cavett at InformDx.com Thu Jan 10 15:21:35 2019 From: Will.Cavett at InformDx.com (Cavett, Will) Date: Thu, 10 Jan 2019 21:21:35 +0000 Subject: [Histonet] Ventana HP IHC dispensors Message-ID: <28b18bd2aacb4659884bc5529d93f034@InformDx.com> I just wanted to see if anyone is having any dispenser issues for your HP IHC from Ventana? This is greatly affecting our workflow across several of our entities. Thanks in advance for your help. Will Cavett, II, MBA, SCT (ASCP) Lab Operations Manager From amarr at med.umich.edu Thu Jan 10 15:27:28 2019 From: amarr at med.umich.edu (Oler, April) Date: Thu, 10 Jan 2019 21:27:28 +0000 Subject: [Histonet] Ventana HP IHC dispensors In-Reply-To: <28b18bd2aacb4659884bc5529d93f034@InformDx.com> References: <28b18bd2aacb4659884bc5529d93f034@InformDx.com> Message-ID: <32adbb916e9c4cd19583c8af4b04ac59@med.umich.edu> Hi Will, I've been seeing sticking dispensers in several IView Blue ISH kits, an Ultraview Kit, and also a Calretinin. All within the last few weeks. I'm very interested to hear if others are having issues as well. April -----Original Message----- From: Cavett, Will via Histonet Sent: Thursday, January 10, 2019 4:22 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Ventana HP IHC dispensors External Email - Use Caution I just wanted to see if anyone is having any dispenser issues for your HP IHC from Ventana? This is greatly affecting our workflow across several of our entities. Thanks in advance for your help. Will Cavett, II, MBA, SCT (ASCP) Lab Operations Manager _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues From tabari.baker at roche.com Thu Jan 10 16:32:42 2019 From: tabari.baker at roche.com (Baker, Tabari) Date: Thu, 10 Jan 2019 17:32:42 -0500 Subject: [Histonet] Ventana HP IHC dispensors In-Reply-To: <28b18bd2aacb4659884bc5529d93f034@InformDx.com> References: <28b18bd2aacb4659884bc5529d93f034@InformDx.com> Message-ID: Hi Will and April, My name is Tabari Baker and I am a Scientific Affairs Manager for Roche Diagnostics. I am in a non-commercial, non-sales role. I am interested in hearing more about your continued issues with sticking dispensers, and want to help get this resolved. Please reach out to me directly so that I can assist you with quickly rectifying these issues. Thanks in advance. Best, TB On Thu, Jan 10, 2019 at 4:41 PM Cavett, Will via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I just wanted to see if anyone is having any dispenser issues for your HP > IHC from Ventana? This is greatly affecting our workflow across several of > our entities. Thanks in advance for your help. > > Will Cavett, II, MBA, SCT (ASCP) > Lab Operations Manager > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- *Tabari Baker, Ph.D.* Scientific Affairs Manager Roche Diagnostics Corporation Tissue Diagnostics 9115 Hague Road Indianapolis IN 46256 M: 202 705 8900 Email: tabari.baker at roche.com www.roche.com Confidentiality Note: This message is intended only for the use of the named recipients and may contain confidential and/or privileged information. If you are not the intended recipient, please contact the sender and delete this message. Any unauthorized use of information contained in this message is prohibited. From tmcampbe at fmh.org Fri Jan 11 07:25:20 2019 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Fri, 11 Jan 2019 13:25:20 +0000 Subject: [Histonet] New Lab Message-ID: Hello, I am a small GI lab and our facility is moving to a brand new building! The building has yet to be built so I have full control on how to design my lab. I will have about 1000-1500 sq ft. I was just wondering if anyone had any tips for me? One thing I want to change is the type of flooring. Right now we have the normal 12 x 12 in tiles that are built in most buildings. Is there any other type of flooring that is better especially when dealing with the paraffin? And the fact that I am scraping the floors? I use to work at the local hospital here and they remodeled the lab there and they put vinyl flooring in that is supposed to be made for labs. Come to find out during the first xylene spill that xylene melts vinyl! So I know I don't want to go that route. Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 CONFIDENTIALITY NOTICE: This electronic mail transmission and any accompanying data files is confidential and is intended exclusively for the individual or entity to which it is addressed. The communication may contain information that is proprietary, privileged or confidential or otherwise legally exempt from disclosure. If you are not the named addressee or you otherwise have received this message in error, you are not authorized to read, print, copy or disseminate this message or any part of it. If you have received this message in error, please notify the sender immediately by email and delete all copies of this message. Receipt by anyone other than the named addressee is not a waiver of any attorney-client work product or other applicable privilege. From Richard.Cartun at hhchealth.org Fri Jan 11 09:06:03 2019 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 11 Jan 2019 15:06:03 +0000 Subject: [Histonet] GD2 Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EAC0F444D@HHCEXCHMB03.hhcsystem.org> Hello everyone. Is anyone doing IHC testing for "GD2" on FFPE tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From priscillan.mohs at gmail.com Fri Jan 11 09:45:28 2019 From: priscillan.mohs at gmail.com (Priscilla Non) Date: Fri, 11 Jan 2019 10:45:28 -0500 Subject: [Histonet] Histology equipment for sale Message-ID: We need to make room and have the following for sale: TBS Minotome Plus 2563 cryostat - compressor works, the microtome is in good condition, but the main board needs to be replaced. Leica SP9000 automatic knife sharpener - sharpening mechanism works but "TABLE" dial mechanism needs to be repaired or replaced. 4 microtome knives, 2 from C.L. Sturkey, 2 from ? - need sharpening, but are in great condition If you're interested please email me. -- Priscilla Non Mohs tech Ali Hendi, M.D., P.C. Mohs & Dermatologic Surgery 5454 Wisconsin Ave., Suite 725 Chevy Chase, MD 20815 phone (301) 986-1006 <%28301%29%20986-1006> fax (301) 986-1056 www.MohsSurgeryMD.com CONFIDENTIALITY NOTICE: This e-mail message, including attachments, is intended only for the addressee named above. It contains information that is privileged, confidential or otherwise protected from use and disclosure. If you are not the intended recipient, you are hereby notified that any review, disclosure, copying, or dissemination of this transmission, or taking of any action in reliance on its contents, or other use is strictly prohibited. If you have received this transmission in error, please reply to the sender listed above immediately and permanently delete this message from your inbox. Thank you for your cooperation. From hegartyhelen at gmail.com Fri Jan 11 12:25:34 2019 From: hegartyhelen at gmail.com (hegartyhelen at gmail.com) Date: Fri, 11 Jan 2019 18:25:34 +0000 Subject: [Histonet] Histonet Digest, Vol 182, Issue 10 In-Reply-To: References: Message-ID: Hi Gareth Regarding the small GI biopsy question using eosin to highlight them for embedding etc Why don?t you try applying Safranin on the biopsies during grossing/dictation. It does not wash out during processing but all GI biopsies will absorb the stain and it makes embedding so much easier. Especially with very fine/tiny biopsies. Helen > On 11 Jan 2019, at 18:00, histonet-request at lists.utsouthwestern.edu wrote: > > Re: Contents of Histonet digest..." > Today's Topics From patpxs at gmail.com Fri Jan 11 12:28:13 2019 From: patpxs at gmail.com (P Sicurello) Date: Fri, 11 Jan 2019 10:28:13 -0800 Subject: [Histonet] New Lab In-Reply-To: References: Message-ID: Hi Tasha, How fun! All I can say is ventilation, ventilation, ventilation. If you can't get proper ventilation I recommend Sentry Air Systems. They have portable fume extractors that use a series of carbon filters, to suck up the xylene or formaldehyde vapors. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Fri, Jan 11, 2019 at 5:37 AM Campbell, Tasha M. via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello, I am a small GI lab and our facility is moving to a brand new > building! The building has yet to be built so I have full control on how > to design my lab. I will have about 1000-1500 sq ft. I was just wondering > if anyone had any tips for me? One thing I want to change is the type of > flooring. Right now we have the normal 12 x 12 in tiles that are built in > most buildings. Is there any other type of flooring that is better > especially when dealing with the paraffin? And the fact that I am scraping > the floors? I use to work at the local hospital here and they remodeled > the lab there and they put vinyl flooring in that is supposed to be made > for labs. Come to find out during the first xylene spill that xylene melts > vinyl! So I know I don't want to go that route. > > > Thanks!! > > > > > Tasha Campbell, B.S.,HTL(ASCP) > Frederick Gastroenterology Associates > 310 W. 9th St. > Frederick, MD 21701 > 301-695-6800 ext. 144 > > > CONFIDENTIALITY NOTICE: This electronic mail transmission and any > accompanying data files is confidential and is intended exclusively for the > individual or entity to which it is addressed. The communication may > contain information that is proprietary, privileged or confidential or > otherwise legally exempt from disclosure. If you are not the named > addressee or you otherwise have received this message in error, you are not > authorized to read, print, copy or disseminate this message or any part of > it. If you have received this message in error, please notify the sender > immediately by email and delete all copies of this message. Receipt by > anyone other than the named addressee is not a waiver of any > attorney-client work product or other applicable privilege. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From patpxs at gmail.com Fri Jan 11 12:30:18 2019 From: patpxs at gmail.com (P Sicurello) Date: Fri, 11 Jan 2019 10:30:18 -0800 Subject: [Histonet] HistoMATE mold cleaner Message-ID: Happy Friday! We pulled our HistoMATE mold cleaner out of the closet and we can't find the user manual. If anyone has one can you send me a copy? I tried looking online and couldn't find anything. It looks pretty simple but I still want to know what it is capable of doing. Thanks oodles and have a nice weekend. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From tbraud at holyredeemer.com Fri Jan 11 13:34:10 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 11 Jan 2019 19:34:10 +0000 Subject: [Histonet] sticking dispensers Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF91D04@HRHEX02-HOS.holyredeemer.local> We still have sticky issues, especially with the 250 HP dispenser, but there have been others. I have given up on Ventana. They don't fix it, don't care, and make you jump through hoops to get help. I'm STILL waiting on my refund 3 months ago from the HP recall where we had to repeat 78 HP tests. Why do we have to contact them and provide a short story's worth of info, only for them to NOT FIX the problem, and hang on to your money. They should be beating down our doors to make their faulty dispensers right. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 3. Ventana HP IHC dispensors (Cavett, Will) 4. Re: Ventana HP IHC dispensors (Oler, April) 5. Re: Ventana HP IHC dispensors (Baker, Tabari) Message: 3 Date: Thu, 10 Jan 2019 21:21:35 +0000 From: "Cavett, Will" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Ventana HP IHC dispensors Message-ID: <28b18bd2aacb4659884bc5529d93f034 at InformDx.com> Content-Type: text/plain; charset="us-ascii" I just wanted to see if anyone is having any dispenser issues for your HP IHC from Ventana? This is greatly affecting our workflow across several of our entities. Thanks in advance for your help. Will Cavett, II, MBA, SCT (ASCP) Lab Operations Manager ------------------------------ Message: 4 Date: Thu, 10 Jan 2019 21:27:28 +0000 From: "Oler, April" To: "'Cavett, Will'" Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] Ventana HP IHC dispensors Message-ID: <32adbb916e9c4cd19583c8af4b04ac59 at med.umich.edu> Content-Type: text/plain; charset="us-ascii" Hi Will, I've been seeing sticking dispensers in several IView Blue ISH kits, an Ultraview Kit, and also a Calretinin. All within the last few weeks. I'm very interested to hear if others are having issues as well. April Message: 5 Date: Thu, 10 Jan 2019 17:32:42 -0500 From: "Baker, Tabari" To: "Cavett, Will" Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Ventana HP IHC dispensors Message-ID: Content-Type: text/plain; charset="UTF-8" Hi Will and April, My name is Tabari Baker and I am a Scientific Affairs Manager for Roche Diagnostics. I am in a non-commercial, non-sales role. I am interested in hearing more about your continued issues with sticking dispensers, and want to help get this resolved. Please reach out to me directly so that I can assist you with quickly rectifying these issues. Thanks in advance. Best, TB From bcooper at chla.usc.edu Fri Jan 11 14:11:26 2019 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Fri, 11 Jan 2019 20:11:26 +0000 Subject: [Histonet] New Lab In-Reply-To: References: Message-ID: <1c38ab50cdb9447fb3252bb5bc38c4a0@chla.usc.edu> Know what else you can't have enough of? Data ports. We just went live with barcoding and tracking and we had to add TONS of them in nearly every room! Good luck! Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357???? Pager: 213-209-0184 bcooper at chla.usc.edu -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, January 11, 2019 10:28 AM To: Campbell, Tasha M. Cc: Histonet Subject: Re: [Histonet] New Lab (EXTERNAL EMAIL) Hi Tasha, How fun! All I can say is ventilation, ventilation, ventilation. If you can't get proper ventilation I recommend Sentry Air Systems. They have portable fume extractors that use a series of carbon filters, to suck up the xylene or formaldehyde vapors. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Fri, Jan 11, 2019 at 5:37 AM Campbell, Tasha M. via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello, I am a small GI lab and our facility is moving to a brand new > building! The building has yet to be built so I have full control on how > to design my lab. I will have about 1000-1500 sq ft. I was just wondering > if anyone had any tips for me? One thing I want to change is the type of > flooring. Right now we have the normal 12 x 12 in tiles that are built in > most buildings. Is there any other type of flooring that is better > especially when dealing with the paraffin? And the fact that I am scraping > the floors? I use to work at the local hospital here and they remodeled > the lab there and they put vinyl flooring in that is supposed to be made > for labs. Come to find out during the first xylene spill that xylene melts > vinyl! So I know I don't want to go that route. > > > Thanks!! > > > > > Tasha Campbell, B.S.,HTL(ASCP) > Frederick Gastroenterology Associates > 310 W. 9th St. > Frederick, MD 21701 > 301-695-6800 ext. 144 > > > CONFIDENTIALITY NOTICE: This electronic mail transmission and any > accompanying data files is confidential and is intended exclusively for the > individual or entity to which it is addressed. The communication may > contain information that is proprietary, privileged or confidential or > otherwise legally exempt from disclosure. If you are not the named > addressee or you otherwise have received this message in error, you are not > authorized to read, print, copy or disseminate this message or any part of > it. If you have received this message in error, please notify the sender > immediately by email and delete all copies of this message. Receipt by > anyone other than the named addressee is not a waiver of any > attorney-client work product or other applicable privilege. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From c.tague at Pathologyarts.com Fri Jan 11 15:40:59 2019 From: c.tague at Pathologyarts.com (Curt) Date: Fri, 11 Jan 2019 21:40:59 +0000 Subject: [Histonet] Quality Manager Message-ID: <9C8F910F72893643B3C3793C3D67132B94D06B6E@PATHOLOGYSERVER.pathologyarts.local> I am looking for a part time person to come in and evaluate our entire system from top to bottom, to get all our policies and procedures compliant if there is a deficiency. This position will require more time up front then, once systems are in place and everyone is satisfied it will likely require less time, perhaps once every other week or even once a month. The focus is on quality, compliance, training and record keeping. Please contact me directly. Thanks, Curt Tague | President/CEO PATHOLOGY ARTS | 1159 Pomona Road, Suite E, Corona, CA 92882 | 951.270.0605 Office 949.246.4402 Cell |http://www.pathologyarts.com/I c.tague at pathologyarts.com | CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. From rsrichmond at gmail.com Sat Jan 12 13:52:01 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 12 Jan 2019 14:52:01 -0500 Subject: [Histonet] Opinion on dye on biopsies In-Reply-To: References: Message-ID: Gareth Davis asked about dyes to use to mark small GI biopsy specimens to make sure they're recovered during embedding. I've had good results marking small specimens with the solution of safranin O that's used in the microbiologists' Gram stain. Go to the micro lab and ask for a small amount of it and try it. Do not use eosin on biopsy specimens. Eosin's brilliant fluorescence makes it very difficult to do any kind of fluorescent stain on the sections. (It also doesn't work as well as safranin, which isn't fluorescent.) Another necessary procedure at the gross desk: fill out a log sheet that records the number of specimens you put into the cassette, and have that log sheet in front of you when you embed. (I've had a lot of histotechs flatly refuse to do this.) I like those little blue foam pads you put in the cassette and put the small specimens on. I usually cut them in two before putting them in the cassette. Bob Richmond Samurai Pathologist Maryville TN From rsrichmond at gmail.com Sat Jan 12 13:58:19 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 12 Jan 2019 14:58:19 -0500 Subject: [Histonet] Eosin on processor for biopsies In-Reply-To: References: Message-ID: Gareth Davis asked about marking small GI specimens with dye when grossing them. I've used safranin O - the solution the microbiologists use in the Gram stain. If you walk down the hall to the micro lab you can get a small amount to try out. Do not use eosin for this purpose. Eosin's brilliant fluorescence makes it very difficult to do any fluorescent stain (such as FISH) on the sections. Safranin O isn't fluorescent. It also works a lot better than eosin. Equally important is to log how many specimens you put in the cassette when you're grossing, and to have that log in front of you when you embed. (I've had a lot of histotechs flatly refuse to do this.) I favor those little blue foam pads to put small specimens on. I usually cut them in two, so that I use a total of only one in each cassette. Bob Richmond Samurai Pathologist Maryville TN From skumar at nyx.net Mon Jan 14 11:53:15 2019 From: skumar at nyx.net (Kumar Sripathirathan) Date: Mon, 14 Jan 2019 10:53:15 -0700 (MST) Subject: [Histonet] Equipment for sale Message-ID: Hello, We are restruturing and have the following equipment for sale on best offer. If you have questions please e-mail or call us at the contact information provided below. Thanks. Kumar Microtome-Accucut- Sakura SRM200CW Microtome-Micron-Thermoscientific- Micron HM450 Tissue Tek VIP2000-Automated tissue processing unit Tissue Tek Thermal console Tissue Tek Dispensing console Tissue Tek Cryo console Sakura-Automated slide stainer- DRS601 Microscope IV900- Binocular w/movable stage w/light source Microscope Zeiss- Invertoscope -- 865, Research Parkway, Suite 415, Oklahoma City, OK 73104 E-mail: skumar at nyx.net Ph: 405.384.8580 From john.garratt at ciqc.ca Mon Jan 14 17:40:50 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Mon, 14 Jan 2019 23:40:50 +0000 Subject: [Histonet] Opinion on dye on biopsies In-Reply-To: References: Message-ID: <5rUmCPYRGYVZbNK4Lqt89QBTKJ-UcNi3Vwx39pQ8yKliLGKpNBHm5A7X5T7brmSHRFXH4Ay8VTUXKcI6T5kpkX5OJ1ptdiVpsroZLTft1NE=@ciqc.ca> Be aware that validation of IHC should be performed if you are changing your processing protocol by adding a dye to the reagents or to a pre-processed tissue. Be cautious! John On Saturday, January 12, 2019 11:52 AM, Bob Richmond via Histonet wrote: > Gareth Davis asked about dyes to use to mark small GI biopsy specimens to > make sure they're recovered during embedding. > > I've had good results marking small specimens with the solution of safranin > O that's used in the microbiologists' Gram stain. Go to the micro lab and > ask for a small amount of it and try it. > > Do not use eosin on biopsy specimens. Eosin's brilliant fluorescence makes > it very difficult to do any kind of fluorescent stain on the sections. (It > also doesn't work as well as safranin, which isn't fluorescent.) > > Another necessary procedure at the gross desk: fill out a log sheet that > records the number of specimens you put into the cassette, and have that > log sheet in front of you when you embed. (I've had a lot of histotechs > flatly refuse to do this.) > > I like those little blue foam pads you put in the cassette and put the > small specimens on. I usually cut them in two before putting them in the > cassette. > > Bob Richmond > Samurai Pathologist > Maryville TN > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Mon Jan 14 18:12:27 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Mon, 14 Jan 2019 19:12:27 -0500 Subject: [Histonet] Opinion on dye on biopsies In-Reply-To: <5rUmCPYRGYVZbNK4Lqt89QBTKJ-UcNi3Vwx39pQ8yKliLGKpNBHm5A7X5T7brmSHRFXH4Ay8VTUXKcI6T5kpkX5OJ1ptdiVpsroZLTft1NE=@ciqc.ca> References: <5rUmCPYRGYVZbNK4Lqt89QBTKJ-UcNi3Vwx39pQ8yKliLGKpNBHm5A7X5T7brmSHRFXH4Ay8VTUXKcI6T5kpkX5OJ1ptdiVpsroZLTft1NE=@ciqc.ca> Message-ID: John Garratt notes: >>Be aware that validation of IHC should be performed if you are changing your processing protocol by adding a dye to the reagents or to a pre-processed tissue. Be cautious!<< I don't think safranin O would be a problem, but a good idea nonetheless! On Mon, Jan 14, 2019 at 6:40 PM John Garratt wrote: > Be aware that validation of IHC should be performed if you are changing > your processing protocol by adding a dye to the reagents or to a > pre-processed tissue. Be cautious! > > > John > > > > On Saturday, January 12, 2019 11:52 AM, Bob Richmond via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Gareth Davis asked about dyes to use to mark small GI biopsy specimens to > > make sure they're recovered during embedding. > > > > I've had good results marking small specimens with the solution of > safranin > > O that's used in the microbiologists' Gram stain. Go to the micro lab and > > ask for a small amount of it and try it. > > > > Do not use eosin on biopsy specimens. Eosin's brilliant fluorescence > makes > > it very difficult to do any kind of fluorescent stain on the sections. > (It > > also doesn't work as well as safranin, which isn't fluorescent.) > > > > Another necessary procedure at the gross desk: fill out a log sheet that > > records the number of specimens you put into the cassette, and have that > > log sheet in front of you when you embed. (I've had a lot of histotechs > > flatly refuse to do this.) > > > > I like those little blue foam pads you put in the cassette and put the > > small specimens on. I usually cut them in two before putting them in the > > cassette. > > > > Bob Richmond > > Samurai Pathologist > > Maryville TN > > > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From garethdavisyuma at gmail.com Tue Jan 15 08:25:41 2019 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Tue, 15 Jan 2019 07:25:41 -0700 Subject: [Histonet] Eosin on biopsy tissue Message-ID: Thanks for all the responses, I will keep the eosin in alcohol for now. Don't worry, I will follow all CAP rules. Thanks again. -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From relia1 at earthlink.net Tue Jan 15 09:01:14 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 15 Jan 2019 10:01:14 -0500 Subject: [Histonet] INCYMI My latest article in the NSH Career Newsletter - Reference Checking Do' and Don'ts Message-ID: <006601d4ace3$29076090$7b1621b0$@earthlink.net> Hi Histonetters, ICYMI - In case you missed it!! I am so excited!! My latest article has been published in the NSH Quarterly Career Center Newsletter under my byline: Make the Cut I have been writing for the NSH Career newsletter on a quarterly basis and a few months ago in one of my emails I asked for topics of interest in anticipation of this column. Thanks again for the suggestions and please keep them coming!! My latest article is about: Reference Checking for job seekers, People giving references and Employers seeking references. Here is the link to the article: https://contentsharing.net/actions/email_web_version.cfm?recipient_id=356670 7953 &message_id=16484336&user_id=NSH%5F&group_id=5202329&jobid=43083480 Histonetters, if you have a minute to read it I would love to hear what you think. If you can't get to the article with this link let me know and I will send you a copy of it. I also have some exciting job opportunities to pass along. Please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral reward! I LOVE TO GIVE REFERRAL REWARDS!! SOME of these are RELIA exclusives. MOST of these offer Sign- on Bonuses and/or Relocation Assistance ALL of these Companies offer excellent compensation, benefits and great environments, opportunity for growth and great people to work with. AND THEY ALL ARE READY TO HIRE!! We have amazing opportunities nationwide! Here are a few of the locations with new positions. Histology Spotlight Opportunity: Histology Territory Sales Manager Texas Marketing/Sales Representative for the state of Texas and part of the rest of the Southwest region. Strong histology and leadership experience are required and previous sales experience is a plus. This is a well-known and well-liked purveyor of histology related products. My client is offering a very competitive compensation package, an excellent support network and an amazing opportunity for an experienced Rep OR someone eager to enter the field!! Histology Territory Sales Manager Ohio Marketing/Sales Representative for Ohio and part of the rest of the Midwest region. Strong histology and leadership skills are required and previous sales experience is a plus. This is a well-known and well-liked purveyor of histology related products. My client is offering a very competitive compensation package, an excellent support network and an amazing opportunity for an experienced Rep OR someone eager to enter the field!! I have exciting histology opportunities in the following areas: Western MA (close to NY border!)- No NY LICENSE required!! Maryland North Carolina Virginia Georgia Texas Tennessee California Alabama My clients offer competitive pay rates, excellent benefits and in most cases Relocation assistance or a sign on bonus. They are ready to interview and hire!!! What more could you ask for? If You or Anyone You Know Might Be Interested In a New Opportunity, Please Contact Me ASAP If you want to chat ASAP call or text me on my cell At 407-353-5070. If you want some additional information or to set up a time to chat please call me toll free at 866-607-3542 or email me at relia1 at earthlink.net My clients are ready to interview and hire right away!!! And my phone is still ringing off the hook so if you don't see the location you want yet drop me a line so I can let you know when something DOES come up. I could be talking to a client about your next opportunity RIGHT NOW!! Have a great day. I look forward to hearing back from you. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Lindsay.A.Wilson at uth.tmc.edu Tue Jan 15 12:55:00 2019 From: Lindsay.A.Wilson at uth.tmc.edu (Wilson, Lindsay A) Date: Tue, 15 Jan 2019 18:55:00 +0000 Subject: [Histonet] Enzymatic Antigen Retrieval for VEGF Message-ID: <47C62412-B317-48FB-AB92-23AB8AA17513@uth.tmc.edu> Good afternoon, Has anyone used enzymatic antigen retrieval for VEGF, successfully? If so, what product and protocol did you follow? Thanks, Lindsay Wilson, LVT, RLATG Young Laboratory UTHealth | The University of Texas Health Science Center at Houston | School of Dentistry Department of Oral & Maxillofacial Surgery 7500 Cambridge St. | Suite 6510 | Houston, TX 77054 713 486 4360 tel | 713 486 4333 fax From hegartyhelen at gmail.com Wed Jan 16 13:22:58 2019 From: hegartyhelen at gmail.com (hegartyhelen at gmail.com) Date: Wed, 16 Jan 2019 19:22:58 +0000 Subject: [Histonet] Histonet Digest, Vol 182, Issue 15 In-Reply-To: References: Message-ID: <5D53847A-0877-4C90-974B-01664DE003CF@gmail.com> Hi Would anybody care to share some information re What equivalency exams if any are necessary in order to work in Nevada as a Histology lab tech, preferably in a hospital setting? What are the different levels of seniority referred to? I am interested in relocating to Nevada as an international applicant. Helen > On 16 Jan 2019, at 18:00, histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Enzymatic Antigen Retrieval for VEGF (Wilson, Lindsay A) > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From psjayalakshmy at gmail.com Wed Jan 16 19:45:03 2019 From: psjayalakshmy at gmail.com (jayalakshmy p.s) Date: Thu, 17 Jan 2019 07:15:03 +0530 Subject: [Histonet] Restain Message-ID: Hello all, How to restain effectively an old H&E stained faded slide. Thanks From kdavoli at gmail.com Thu Jan 17 08:52:13 2019 From: kdavoli at gmail.com (Kate Davoli) Date: Thu, 17 Jan 2019 09:52:13 -0500 Subject: [Histonet] Faded Masson trichrome Message-ID: This is quite a puzzle. I ran a handful of slides in the SAME manual run for my Masson trichrome stain (one glass caddy of slides, passing through single troughs of dyes). I've just coverslipped them and half the slides have the expected bright high contrast coloring that I usually see, and half the slides are pale/faded for all three colors. The slides themselves came from different experiments (same wax, same thickness, but cut and de-waxed at different times) ... could it be that the paler set was not fully de-waxed? Has anyone else seen this? I would appreciate any help you can provide. Thanks! Katherine Davoli, BA, HTL(ASCP)CM Supervisor & Lab Manager, Tissue Culture & Histology Core Module Ophthalmic and Visual Sciences Research Center Department of Ophthalmology, University of Pittsburgh Mail Stop Code: EEI010901 930 Eye & Ear Institute, 203 Lothrop Street Pittsburgh, PA 15213 (412) 647-8256 davolika at upmc.edu and kdavoli at gmail.com From Linda.Margraf at cookchildrens.org Thu Jan 17 14:51:37 2019 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Thu, 17 Jan 2019 20:51:37 +0000 Subject: [Histonet] FW: Histonet submission In-Reply-To: References: Message-ID: Here is a message I am posting for Christine/ Chrissy.... From: Altemus, Christine [mailto:Christine.Altemus at ascension.org] Sent: Thursday, January 17, 2019 12:02 PM To: Linda Margraf Subject: Histonet submission Hello - We currently use the following Pulmo Panel stains from Biocare: Desmoglein 3 + Napsin A, TTF-1 + CK5 and p63 + TRIM29 cocktails. We have plenty of lung adenocarcinoma control tissue but our pathologist is requesting squamous lung control. If you can help, please let me know - thank you for the response. Chrissy @ St. Mary's Healthcare - Amsterdam, NY Christine.Altemus at ascension.org> From DavidKemlerLLC at msn.com Fri Jan 18 06:51:15 2019 From: DavidKemlerLLC at msn.com (David) Date: Fri, 18 Jan 2019 12:51:15 +0000 Subject: [Histonet] IHC Manual Staining Message-ID: Hello Histoneters - Does anyone still do manual IHC's? If so, which IHC staining kits would you recommend? Thanks. Yours, Dave From jeanine.ronkowski at yahoo.com Fri Jan 18 12:58:02 2019 From: jeanine.ronkowski at yahoo.com (Jeanine Ronkowski) Date: Fri, 18 Jan 2019 18:58:02 +0000 (UTC) Subject: [Histonet] Histonet - IHC Manual staining References: <1282106629.1842099.1547837882252.ref@mail.yahoo.com> Message-ID: <1282106629.1842099.1547837882252@mail.yahoo.com> Hi Dave:Our lab has used reagents from Biocare Medical for a very long time. They?re packaged in 6ml and 25 ml vials and ideal for hand staining. And their technical support is great tooJeanine Hello Histoneters - Does anyone still do manual IHC's? If so, which IHC staining kits would you recommend? Thanks. Yours, Dave Sent from Yahoo Mail for iPhone From aeck at dh.org Fri Jan 18 13:03:59 2019 From: aeck at dh.org (Eck, Allison) Date: Fri, 18 Jan 2019 19:03:59 +0000 Subject: [Histonet] LIS order entry Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64AA7F1D678@DH-MAIL02.dhorg.org> Hello Histonet! I am curious if anyone would be willing to share their computer order entry experiences. Our current process is the order is placed on the floor, in the OR, etc and a paper copy is sent down to us with the specimen. Once we receive the order, we have to manually re enter all of the data into the pathology order. There has been some discussion about the possibility of having orders cross over to us. I was wondering (regardless of LIS) what processes are out there. Is everyone doing it the same as us or do most labs have the order intergration? Thanks Allison From cforster at umn.edu Fri Jan 18 14:05:23 2019 From: cforster at umn.edu (Colleen Forster) Date: Fri, 18 Jan 2019 14:05:23 -0600 Subject: [Histonet] Histonet - IHC Manual staining In-Reply-To: <1282106629.1842099.1547837882252@mail.yahoo.com> References: <1282106629.1842099.1547837882252.ref@mail.yahoo.com> <1282106629.1842099.1547837882252@mail.yahoo.com> Message-ID: Hello Dave, I still do a lot of manual work and I also use Biocare reagents. They work well and their customer service is very good. Colleen Forster On Fri, Jan 18, 2019 at 12:58 PM Jeanine Ronkowski via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Dave:Our lab has used reagents from Biocare Medical for a very long > time. They?re packaged in 6ml and 25 ml vials and ideal for hand staining. > And their technical support is great tooJeanine > > Hello Histoneters - > > Does anyone still do manual IHC's? If so, which IHC staining kits would > you recommend? Thanks. > > Yours, > Dave > > Sent from Yahoo Mail for iPhone > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at holml at umn.edu * From DavidKemlerLLC at msn.com Sat Jan 19 07:35:05 2019 From: DavidKemlerLLC at msn.com (David) Date: Sat, 19 Jan 2019 13:35:05 +0000 Subject: [Histonet] Many Thanks Message-ID: Thank you all for your information on IHC manual staining. I appreciate everyone's prompt attention. Yours, Dave From mgflem at gmail.com Mon Jan 21 09:26:49 2019 From: mgflem at gmail.com (Matthew Fleming) Date: Mon, 21 Jan 2019 09:26:49 -0600 Subject: [Histonet] Part-time histotech needed near Milwaukee, WI Message-ID: A part-time histotech is needed for an independent dermatopathology lab in Brown Deer, WI. Hours are flexible and negotiable and salary is highly competitive. Please address inquiries to the lab director: Dr. Matthew Fleming Fleming Dermatopathology 4324 W Bradley Road Brown Deer, WI 53223 mgflem at gmail.com From Richard.Cartun at hhchealth.org Mon Jan 21 14:19:43 2019 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 21 Jan 2019 20:19:43 +0000 Subject: [Histonet] IHC Billing - 88342 vs. 88341 Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EAC10366F@HHCEXCHMB03.hhcsystem.org> For those of you doing clinical IHC testing, have you been able to automate the billing of IHC (88342 vs. 88341) or do you still manual bill? Our IT staff wants to build-in a "88342" and a ""88341" for every antibody that we run and then have the pathologist select the appropriate one for billing. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From aperl at cmmedical.com Mon Jan 21 15:28:55 2019 From: aperl at cmmedical.com (Perl , Alison) Date: Mon, 21 Jan 2019 21:28:55 +0000 Subject: [Histonet] IHC Billing - 88342 vs. 88341 In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2EAC10366F@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2EAC10366F@HHCEXCHMB03.hhcsystem.org> Message-ID: Hi Dr Cartun That was our worst-case scenario, but our LIS was able to build logic so that it will convert 88342 to 88341 after the 1st, for each specimen (rather than case). It's been working well for the past 6 months or so... Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at cmmedical.com -----Original Message----- From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, January 21, 2019 3:20 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] IHC Billing - 88342 vs. 88341 For those of you doing clinical IHC testing, have you been able to automate the billing of IHC (88342 vs. 88341) or do you still manual bill? Our IT staff wants to build-in a "88342" and a ""88341" for every antibody that we run and then have the pathologist select the appropriate one for billing. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and its attachments, if any, may contain confidential or proprietary information and are intended solely for authorized use by the intended recipient(s) only. Any other use of this email is prohibited. If you have received this email in error, you are hereby notified that any retention, disclosure, copying, forwarding, distribution (in whole or in part and whether electronically, written and/or orally) and/or taking of any action in reliance on this email, its contents and/or any attachments thereto is strictly prohibited. If you received this email in error, please notify the sender by replying to this message and permanently delete this email, and any attachments thereto, from your system immediately. From Browj54 at LabCorp.com Tue Jan 22 10:03:17 2019 From: Browj54 at LabCorp.com (Browning, Jeffrey) Date: Tue, 22 Jan 2019 16:03:17 +0000 Subject: [Histonet] Histotech & Histology Team Leader opportunities in Raleigh/Durham, NC Message-ID: LabCorp Center for Molecular Biology and Pathology in RTP, NC is looking for histotechs for 2nd and 3rd shift and for a histology team leader for 2nd shift. Please see the below links if you are interested: 3rd shift histotech: https://jobs.labcorp.com/job/research-triangle-park/histotechnician/668/9766681 2nd shift histotech: https://jobs.labcorp.com/job/research-triangle-park/histotechnician/668/9981002 2nd shift team leader: https://jobs.labcorp.com/job/research-triangle-park/histology-team-leader/668/10502600 Thanks, Jeff Browning, HTL(ASCP)QIHC Histology Supervisor ? 1st Shift? 1912 TW Alexander Drive RTP, NC 27709 919-361-7232 Browj54 at labcorp.com -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722. From mwich at 7thwavelabs.com Tue Jan 22 10:04:12 2019 From: mwich at 7thwavelabs.com (Michele Wich) Date: Tue, 22 Jan 2019 16:04:12 +0000 Subject: [Histonet] FLAG control slides for IHC Message-ID: <4ADAF16E8518764ABF9DCEB73129AA758617BBEF@WAVE-EMAIL.7thwave.local> Does anyone know of a commercially available source for FLAG (DYKDDDDK) IHC control slides? Thank you. Michele From andy at vabderm.com Tue Jan 22 11:36:30 2019 From: andy at vabderm.com (andy at vabderm.com) Date: Tue, 22 Jan 2019 10:36:30 -0700 Subject: [Histonet] Looking for reasonable biomedical company in Colorado Message-ID: <002b01d4b279$0337c300$09a74900$@vabderm.com> Hey Histonetters, I am currently looking for a biomedical company that can work with a wide array of equipment as we are not just a leica or sakura lab. We have a bond III, leica st5020 stainer, sakura film coverslipper, microm microtome, VIP5 processor, microm cryostat, sakura embedding station. We are a new lab and have service contracts that end in October this year, we current use Rankin out of MI as they were the company that furnished the lab. Their prices seem very high so id like to shop around for a company that is closer to the lab in Glenwood Springs CO. If anyone hs any leads please let me know. TIA Andy Fortune From dhill32 at comcast.net Tue Jan 22 13:43:11 2019 From: dhill32 at comcast.net (David Hill) Date: Tue, 22 Jan 2019 13:43:11 -0600 Subject: [Histonet] Job openings Message-ID: <4EE23AD8-B06B-4BDD-8540-095BEB401FEB@comcast.net> FT HT/HTL and FT PA positions available at Trumbull Laboratories. See trumbulllabs.com for more info. Sent from my iPhone From Caroline.Pratt at uphs.upenn.edu Tue Jan 22 14:28:46 2019 From: Caroline.Pratt at uphs.upenn.edu (Pratt, Caroline) Date: Tue, 22 Jan 2019 20:28:46 +0000 Subject: [Histonet] Cameras in the Laboratory Message-ID: <14D6A469D20B4F4AACC47C3E973C3FA801742278@UPHMASPHI030.UPHS.PENNHEALTH.PRV> If anyone is using cameras in the laboratory. Can you share your experience with me and the placement/use/effectiveness, etc? Caroline.pratt at uphs.upenn.edu. Thank you! Caroline M. Pratt, MBA Practice Administrator Dermpath 3020 Market Street, Ste 201 Philadelphia, PA 19104 Phone 215-349-8178 Cell 610-800-1381 Fax 215-662-6150 From lblazek at digestivespecialists.com Tue Jan 22 15:03:36 2019 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Tue, 22 Jan 2019 21:03:36 +0000 Subject: [Histonet] Cameras in the Laboratory In-Reply-To: <14D6A469D20B4F4AACC47C3E973C3FA801742278@UPHMASPHI030.UPHS.PENNHEALTH.PRV> References: <14D6A469D20B4F4AACC47C3E973C3FA801742278@UPHMASPHI030.UPHS.PENNHEALTH.PRV> Message-ID: If you could share the responses you get I'd appreciate it. We just built a new facility. There are cameras everywhere. They are in the lab and I hate them but you soon forget that they are there. They cover almost all of the lab. There is a small area that isn't covered and we call it the personal scratching area. LOL . My office does not have a camera in it. Linda -----Original Message----- From: Pratt, Caroline via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, January 22, 2019 3:29 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cameras in the Laboratory If anyone is using cameras in the laboratory. Can you share your experience with me and the placement/use/effectiveness, etc? Caroline.pratt at uphs.upenn.edu. Thank you! Caroline M. Pratt, MBA Practice Administrator Dermpath 3020 Market Street, Ste 201 Philadelphia, PA 19104 Phone 215-349-8178 Cell 610-800-1381 Fax 215-662-6150 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Tue Jan 22 15:58:56 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 22 Jan 2019 21:58:56 +0000 Subject: [Histonet] Cameras in the Laboratory In-Reply-To: <14D6A469D20B4F4AACC47C3E973C3FA801742278@UPHMASPHI030.UPHS.PENNHEALTH.PRV> References: <14D6A469D20B4F4AACC47C3E973C3FA801742278@UPHMASPHI030.UPHS.PENNHEALTH.PRV> Message-ID: Caroline, The only cameras in our lab and the histo lab are pointed at the tissue processor screens so we can log in and see what is going on when we get an error alert. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Pratt, Caroline via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, January 22, 2019 12:29 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cameras in the Laboratory If anyone is using cameras in the laboratory. Can you share your experience with me and the placement/use/effectiveness, etc? Caroline.pratt at uphs.upenn.edu. Thank you! Caroline M. Pratt, MBA Practice Administrator Dermpath 3020 Market Street, Ste 201 Philadelphia, PA 19104 Phone 215-349-8178 Cell 610-800-1381 Fax 215-662-6150 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aperl at cmmedical.com Wed Jan 23 07:31:26 2019 From: aperl at cmmedical.com (Perl , Alison) Date: Wed, 23 Jan 2019 13:31:26 +0000 Subject: [Histonet] IHC Billing - 88342 vs. 88341 In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2EAC10366F@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2EAC10366F@HHCEXCHMB03.hhcsystem.org> Message-ID: <39d6ad6d1a13402196a73d3201b94ba5@MK-EXMB02.mkmg.com> Morning everyone I got a couple of inquiries about my LIS. We use IntelliPath from NetSoft; we're a physician-owned lab with separate clinical and pathology labs. They were able to customize logic for us to make sure IHC and SS get billed correctly without manual intervention 99% of the time. We're working on an interface, but it currently just exports to an Excel file that we send to Billing. Hope that helps! If you have other questions just email me Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at cmmedical.com -----Original Message----- From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, January 21, 2019 3:20 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] IHC Billing - 88342 vs. 88341 For those of you doing clinical IHC testing, have you been able to automate the billing of IHC (88342 vs. 88341) or do you still manual bill? Our IT staff wants to build-in a "88342" and a ""88341" for every antibody that we run and then have the pathologist select the appropriate one for billing. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and its attachments, if any, may contain confidential or proprietary information and are intended solely for authorized use by the intended recipient(s) only. Any other use of this email is prohibited. If you have received this email in error, you are hereby notified that any retention, disclosure, copying, forwarding, distribution (in whole or in part and whether electronically, written and/or orally) and/or taking of any action in reliance on this email, its contents and/or any attachments thereto is strictly prohibited. If you received this email in error, please notify the sender by replying to this message and permanently delete this email, and any attachments thereto, from your system immediately. From relia1 at earthlink.net Wed Jan 23 10:55:56 2019 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 23 Jan 2019 11:55:56 -0500 Subject: [Histonet] Are you a Morning Person or a Night Owl? Message-ID: <008c01d4b33c$82438620$86ca9260$@earthlink.net> Hello Histonetters, Sooo How do you like your shift? Are you a Night Owl on NIGHTS? Or A Morning Person on DAYS? If you answered I love my shift and Yes ? Congratulations!!! On the other hand If you answered NOT My Favorite Shift and No I am a Night Owl on DAYS Or A Morning Person on NIGHTS We need to talk! Histonetters, I need to know if you want to change shifts so I can contact you when a position comes up!! Right now I have day and night shift positions! Here they are: Here is the Super Sizzlin Hot Spotlight Opportunity! Anatomic Pathology Manager ? A well-known and respected private histology reference lab located in Milwaukee, WI has engaged RELIA in their search for an AP Manager. They are looking for an ASCP certified HT/HTL with strong management and troubleshooting skills. This is a one of a kind unique opportunity for someone who is really geared towards advancement!! These are the other Sizzling HOT Super Opportunities!! Leadership Opportunities: Histology Territory Sales Manager ? TX Histology Territory Sales Manager ? OH AP Manager ? Milwaukee, WI **1st Shift Opportunities: Histotech ? Virginia ? DAYS!! Histotech ? Massachusetts - DAYS!! Histotech - Maryland- DAYS!! Histotech Wisconsin - DAYS!! Histotech ? San Diego - DAYS!! **3rd Shift Opportunities: Dermpath Histotech ? Alabama-NIGHTS!! Grossing Histotech ? Tennessee- NIGHTS!! Dermpath Histotech ? Wisconsin- NIGHTS!! Histotech ? Tallahassee, FL- NIGHTS!! All of these positions are full time and permanent!! My clients offer excellent compensation, benefits and in most cases either relocation or a sign-on bonus. If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I place someone you refer You will earn a referral fee. If you are interested in any of these opportunities CALL /TEXT MY CELL ASAP!!! At 407-353-5070. I can also be reached toll free at the office at 866-607-3542 or at relia1 at earthlink.net to set up a time to talk at your convenience! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From c.tague at Pathologyarts.com Wed Jan 23 14:54:32 2019 From: c.tague at Pathologyarts.com (Curt) Date: Wed, 23 Jan 2019 20:54:32 +0000 Subject: [Histonet] BRCA1 on Benchmark Ultra Message-ID: <9C8F910F72893643B3C3793C3D67132B94D0B4A3@PATHOLOGYSERVER.pathologyarts.local> Anyone getting good results, I'm trying to determine which clone/vendor to use... better to ask before I waste a bunch of time and money... any suggestions or recommendations? Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. From akuvetpatoloji at gmail.com Thu Jan 24 12:07:00 2019 From: akuvetpatoloji at gmail.com (=?UTF-8?B?QUvDnFZFVCBQQVRPTE9KxLA=?=) Date: Thu, 24 Jan 2019 21:07:00 +0300 Subject: [Histonet] BVD positve block. Message-ID: Hello Dear Histonetters, I need a positive block of BVD (Bovine Virus Diarrhea) disease virus need. I would be very grateful if anyone help me. Thank you very much. -- Dr. M. Fatih Bozkurt AK? Veteriner Fak?ltesi Analiz ve Te?his Laboratuvar? Patoloji Birimi - Afyonkarahisar From tbraud at holyredeemer.com Thu Jan 24 12:51:25 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 24 Jan 2019 18:51:25 +0000 Subject: [Histonet] another Ventana dispenser failure Message-ID: <48E053DDF6CE074DB6A7414BA05403F8013BE4D104@HRHEX02-HOS.holyredeemer.local> We have another dispenser failure. Ki-67, Cat# 790-4286, Lot# E17347, Failed on the 9th to last dispense. The plunger was very "sticky" and difficult to depress. Roche, are you listening??? Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From Hans at histologistics.com Thu Jan 24 12:52:44 2019 From: Hans at histologistics.com (Hans B Snyder) Date: Thu, 24 Jan 2019 13:52:44 -0500 Subject: [Histonet] BRCA1 on Benchmark Ultra (Curt) In-Reply-To: References: Message-ID: Hello, We've had good results with Calbiochem antibody Cat#OP92 clone MS110, dilution 1:500. Good luck Hans B Snyder JMD Histology & Histologistics Inc. 151 W Main Street Dudley, MA 01571 Lab - 508-461-7207 C- 508-308-7800 www.histologistics.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. On Thu, Jan 24, 2019 at 1:23 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. BRCA1 on Benchmark Ultra (Curt) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 23 Jan 2019 20:54:32 +0000 > From: Curt > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] BRCA1 on Benchmark Ultra > Message-ID: > > <9C8F910F72893643B3C3793C3D67132B94D0B4A3 at PATHOLOGYSERVER.pathologyarts.local > > > > Content-Type: text/plain; charset="us-ascii" > > Anyone getting good results, I'm trying to determine which clone/vendor to > use... better to ask before I waste a bunch of time and money... any > suggestions or recommendations? > > > Curt > > > > CONFIDENTIALITY NOTE: The information transmitted, including attachments, > is intended only for the person(s) or entity to which it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of, or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this in error, please > contact the sender and destroy any copies of this information. > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 182, Issue 22 > ***************************************** > From Nancy_Schmitt at pa-ucl.com Mon Jan 28 11:10:26 2019 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Mon, 28 Jan 2019 17:10:26 +0000 Subject: [Histonet] glass slides Message-ID: Hello- We are seeing some warping with glass slides from Leica. Looking for recommendation on what you are currently using? Thank you Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From mgiorgi at incdx.com Mon Jan 28 15:53:54 2019 From: mgiorgi at incdx.com (Miranda Giorgi) Date: Mon, 28 Jan 2019 21:53:54 +0000 Subject: [Histonet] Cameras in the Laboratory In-Reply-To: <14D6A469D20B4F4AACC47C3E973C3FA801742278@UPHMASPHI030.UPHS.PENNHEALTH.PRV> References: <14D6A469D20B4F4AACC47C3E973C3FA801742278@UPHMASPHI030.UPHS.PENNHEALTH.PRV> Message-ID: Hello, We have cameras at gross and embedding. They are really helpful for troubleshooting potential errors that would otherwise be difficult to trace. Originally, the staff was nervous about how these cameras would be used but have since realized their value. Miranda Giorgi -----Original Message----- From: Pratt, Caroline via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, January 22, 2019 12:29 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cameras in the Laboratory If anyone is using cameras in the laboratory. Can you share your experience with me and the placement/use/effectiveness, etc? Caroline.pratt at uphs.upenn.edu. Thank you! Caroline M. Pratt, MBA Practice Administrator Dermpath 3020 Market Street, Ste 201 Philadelphia, PA 19104 Phone 215-349-8178 Cell 610-800-1381 Fax 215-662-6150 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700. From Caroline.Pratt at uphs.upenn.edu Mon Jan 28 15:56:03 2019 From: Caroline.Pratt at uphs.upenn.edu (Pratt, Caroline) Date: Mon, 28 Jan 2019 21:56:03 +0000 Subject: [Histonet] Cameras in the Laboratory In-Reply-To: References: <14D6A469D20B4F4AACC47C3E973C3FA801742278@UPHMASPHI030.UPHS.PENNHEALTH.PRV> Message-ID: <14D6A469D20B4F4AACC47C3E973C3FA801744EB1@UPHMASPHI030.UPHS.PENNHEALTH.PRV> Thank you! -----Original Message----- From: Miranda Giorgi Sent: Monday, January 28, 2019 4:54 PM To: Pratt, Caroline Cc: 'histonet at lists.utsouthwestern.edu' Subject: [External] RE: Cameras in the Laboratory Hello, We have cameras at gross and embedding. They are really helpful for troubleshooting potential errors that would otherwise be difficult to trace. Originally, the staff was nervous about how these cameras would be used but have since realized their value. Miranda Giorgi -----Original Message----- From: Pratt, Caroline via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, January 22, 2019 12:29 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cameras in the Laboratory If anyone is using cameras in the laboratory. Can you share your experience with me and the placement/use/effectiveness, etc? Caroline.pratt at uphs.upenn.edu. Thank you! Caroline M. Pratt, MBA Practice Administrator Dermpath 3020 Market Street, Ste 201 Philadelphia, PA 19104 Phone 215-349-8178 Cell 610-800-1381 Fax 215-662-6150 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700. From idimenstein at hotmail.com Tue Jan 29 06:46:40 2019 From: idimenstein at hotmail.com (Izak Dimenstein) Date: Tue, 29 Jan 2019 12:46:40 +0000 Subject: [Histonet] Cameras in the gross room Message-ID: Dear Miranda Giorgi: What is the purpose to have cameras, especially at the embedding station? What kind of cameras do you use? Thank you, Izak Dimenstein From mjdessoye at commonwealthhealth.net Tue Jan 29 08:04:39 2019 From: mjdessoye at commonwealthhealth.net (Dessoye, Michael) Date: Tue, 29 Jan 2019 14:04:39 +0000 Subject: [Histonet] glass slides In-Reply-To: References: Message-ID: That's interesting, we've been seeing warping with the Leica coverslips. I wonder if there's something in common in their manufacturing. We've been using a mixture of Superfrost Plus (Fisher), Basix (Fisher), and Platinum line (Mercedes Medical) all with good results. The 'Basix' seems to be a good all-around non-charged slide that is affordable. Hope this helps! Mike Michael J. Dessoye, M.S.?|?Histology/Toxicology/Special Chemistry Supervisor?|?Commonwealth Health Laboratory Services |?mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 -----Original Message----- From: Nancy Schmitt [mailto:Nancy_Schmitt at pa-ucl.com] Sent: Monday, January 28, 2019 12:10 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] glass slides Hello- We are seeing some warping with glass slides from Leica. Looking for recommendation on what you are currently using? Thank you Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ********************************************************************** Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From relia1 at earthlink.net Tue Jan 29 09:59:31 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 29 Jan 2019 10:59:31 -0500 Subject: [Histonet] Can I Warm You Up With some Sizzlin' Hot Histo Opportunties? Message-ID: <006901d4b7eb$9f852e10$de8f8a30$@earthlink.net> Hello Histonetters, I hope you are having a great week. I wanted to send a quick note to tell you about the positions that I am working on and am most excited about.? Why am I excited about these positions? Because each of these clients was asked if I had someone for you today would you be ready to interview and hire Every One of these clients said YES!!! Here is THE Top! Super Sizzlin Hot Spotlight Opportunity! Histotech ? Norfolk, VA Day shift 9K sign on bonus!! These are the Sizzling HOT Super Leadership Opportunities!! Territory Sales Manager ? Ohio Territory Sales Manager ? Texas AP Manager ? Milwaukee, WI These are the Sizzling HOT Super HT/HTL Opportunities!! Histotech ? Annapolis, MD Histotech ? Norfolk, VA Histotech ? Springfield, MA Histotech ? NYC, NY NY license req. Histotech ? Chattanooga, TN Histotech ? Atlanta, GA Histotech ? Milwaukee, WI All of these positions are full time and permanent!! My clients offer excellent compensation, benefits and in most cases either relocation or a sign-on bonus. Histonetters, if you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I place someone that You refer You will earn a referral fee. If you are interested in any of these opportunities CALL /TEXT MY CELL ASAP!!! At 407-353-5070. I can also be reached toll free at the office at 866-607-3542 or at relia1 at earthlink.net to set up a time to talk at your convenience! Thank you so much for taking the time to read my email. I really appreciate it!! Thanks- Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From mgiorgi at incdx.com Tue Jan 29 10:38:50 2019 From: mgiorgi at incdx.com (Miranda Giorgi) Date: Tue, 29 Jan 2019 16:38:50 +0000 Subject: [Histonet] Cameras in the gross room In-Reply-To: References: Message-ID: Hello Izak, The cameras at embedding show a view of the embedding hot plate and embedders lap. I would be surprised to meet a histo tech who has never experienced a piece of tissue popping out of a cassette when opening it up. The cameras are useful for identifying where the tissue might have jumped to. In addition, should there be any other type of error or floater tissue in a block, the cameras can be reviewed to see where the mystery tissue came from. When trouble shooting source of errors you can also check to see if the techs are following best practices, like cleaning tools after each use and cleaning the hot plates between blocks. Having cameras at gross and embedding allow you to determine at what point floaters could have been introduced in the process or reconcile any discrepancies between gross and embedding. Without the cameras, we could only make our best guess when it comes to troubleshooting. By reviewing camera footage it is easier to identify the real root cause of an issue so that you can improve your process and reduce future errors. In addition to histology, we also have cameras in our accessioning area where the specimens are initially received. I work for a company with multiple labs throughout Washington. We recently opened a new facility and it did not have cameras set up for the first six weeks or our operations. It was a great reminder of how helpful and important these cameras are. We would never go back to a camera free environment. Our cameras are IQeye cameras, and the camera footage is managed by OpenEye video surveillance software. I hope this is helpful information. Miranda Giorgi -----Original Message----- From: Izak Dimenstein via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, January 29, 2019 4:47 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cameras in the gross room Dear Miranda Giorgi: What is the purpose to have cameras, especially at the embedding station? What kind of cameras do you use? Thank you, Izak Dimenstein _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700. From shive003 at umn.edu Tue Jan 29 13:58:13 2019 From: shive003 at umn.edu (Jan Shivers) Date: Tue, 29 Jan 2019 13:58:13 -0600 Subject: [Histonet] guinea pig IHC Message-ID: Has anyone ever performed IHC on frozen sections of guinea pig tissue? I am experiencing an enormous amount of bubbling when doing the peroxidase blocking step, even though I'm only using a 0.3% concentration of H2O2. And when I say 'enormous', I mean it's like continuous champagne bubbles rising out of the tissue, even after 20 minutes in the H2O2 solution. I can't find anything in the literature that mentions guinea pigs having a higher peroxidase content in their tissues. Thanks for any help that anyone can provide. Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003 at umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* From cforster at umn.edu Tue Jan 29 14:12:59 2019 From: cforster at umn.edu (Colleen Forster) Date: Tue, 29 Jan 2019 14:12:59 -0600 Subject: [Histonet] guinea pig IHC In-Reply-To: References: Message-ID: following. I haven't done frozen stains on GP only FFPE...interested in what others have to say. Colleen On Tue, Jan 29, 2019 at 1:58 PM Jan Shivers via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Has anyone ever performed IHC on frozen sections of guinea pig tissue? I > am experiencing an enormous amount of bubbling when doing the peroxidase > blocking step, even though I'm only using a 0.3% concentration of H2O2. > And when I say 'enormous', I mean it's like continuous champagne bubbles > rising out of the tissue, even after 20 minutes in the H2O2 solution. > > I can't find anything in the literature that mentions guinea pigs having a > higher peroxidase content in their tissues. > > Thanks for any help that anyone can provide. > > Jan Shivers > Senior Scientist > IHC/Histology Section Manager > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive003 at umn.edu > > *Confidentiality Notice: This message, together with any attachments, is > intended only for the use of the individual or entity to which it is > addressed and may contain confidential or privileged information. If you > think you have received this message in error, please advise the sender and > then delete this message and any attachments immediately.* > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at holml at umn.edu * From kristyn.ferber at gmail.com Wed Jan 30 13:27:28 2019 From: kristyn.ferber at gmail.com (Kristyn Ferber) Date: Wed, 30 Jan 2019 14:27:28 -0500 Subject: [Histonet] Histotechnologist Needed near Philly! Message-ID: Hello Histo-netters! Busy hospital lab looking for a great histotech! Apply using the link below :) https://careers-towerhealth.icims.com/jobs/23469/histotechnologist/job Have a great day! From madeathridge at pastnashville.com Thu Jan 31 09:11:45 2019 From: madeathridge at pastnashville.com (Maryann Deathridge) Date: Thu, 31 Jan 2019 15:11:45 +0000 Subject: [Histonet] Bleach Regs Message-ID: Hello Histonetters! Has anyone heard of a regulation by CAP, CLIA or OSHA regarding commercial bleach stored under the laboratory sink. My lab routinely has a gallon container of dated/ bio-labeled commercial bleach stored under the sink for cleaning purposes. Daily use to clean special stain coplin jars,etc.. I had a suspicious med tech tell my pathologist that is was against regulations to have it in the lab anytime ! HUH???? Responses welcomed. Have an awesome Thursday! Maryann Deathridge, Lab Manager Pathology Assoc. of St. Thomas 4220 Harding Pike Bldg. SE, Suite 504 Nashville, TN 37205 madeathridge at pastnashville.com From boznpl at aol.com Thu Jan 31 10:13:12 2019 From: boznpl at aol.com (Laurie Redmond) Date: Thu, 31 Jan 2019 16:13:12 +0000 (UTC) Subject: [Histonet] Bleach Regs References: <1362818747.989057.1548951192666.ref@mail.yahoo.com> Message-ID: <1362818747.989057.1548951192666@mail.yahoo.com> We don't store anything under the sinks in our lab per CAP regulations.? I do not understand why, nor do I have the specific checklist question at my fingertips, but we always make sure everything is removed from under the sinks at inspection time. Laurie Redmond -----Original Message----- From: Maryann Deathridge via Histonet To: histonet at lists.utsouthwestern.edu Sent: Thu, Jan 31, 2019 7:18 am Subject: [Histonet] Bleach Regs Hello Histonetters! Has anyone heard of a regulation by CAP, CLIA or OSHA regarding commercial bleach stored under the laboratory sink.? My lab routinely has a gallon container of dated/ bio-labeled commercial bleach stored under the sink for cleaning purposes. Daily use to clean special stain coplin jars,etc..? I had a suspicious med tech tell my pathologist that is was against regulations to have it in the lab anytime ! HUH???? Responses welcomed. Have an awesome Thursday! Maryann? Deathridge, Lab Manager Pathology Assoc. of? St. Thomas 4220 Harding? Pike Bldg. SE,? Suite 504 Nashville, TN? 37205 madeathridge at pastnashville.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Thu Jan 31 10:21:46 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 31 Jan 2019 16:21:46 +0000 Subject: [Histonet] Bleach Regs In-Reply-To: <1362818747.989057.1548951192666@mail.yahoo.com> References: <1362818747.989057.1548951192666.ref@mail.yahoo.com> <1362818747.989057.1548951192666@mail.yahoo.com> Message-ID: Cleaning supplies may be stored under the sink. Bleach is a cleaning agent. We do it and no inspector looking there has ever said anything about it. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Laurie Redmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, January 31, 2019 8:13 AM To: madeathridge at pastnashville.com; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Bleach Regs We don't store anything under the sinks in our lab per CAP regulations.? I do not understand why, nor do I have the specific checklist question at my fingertips, but we always make sure everything is removed from under the sinks at inspection time. Laurie Redmond -----Original Message----- From: Maryann Deathridge via Histonet To: histonet at lists.utsouthwestern.edu Sent: Thu, Jan 31, 2019 7:18 am Subject: [Histonet] Bleach Regs Hello Histonetters! Has anyone heard of a regulation by CAP, CLIA or OSHA regarding commercial bleach stored under the laboratory sink.? My lab routinely has a gallon container of dated/ bio-labeled commercial bleach stored under the sink for cleaning purposes. Daily use to clean special stain coplin jars,etc..? I had a suspicious med tech tell my pathologist that is was against regulations to have it in the lab anytime ! HUH???? Responses welcomed. Have an awesome Thursday! Maryann? Deathridge, Lab Manager Pathology Assoc. of? St. Thomas 4220 Harding? Pike Bldg. SE,? Suite 504 Nashville, TN? 37205 madeathridge at pastnashville.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garethdavisyuma at gmail.com Thu Jan 31 11:52:24 2019 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Thu, 31 Jan 2019 10:52:24 -0700 Subject: [Histonet] Bleach Regs In-Reply-To: References: Message-ID: Hi Maryann, Nosy Med techs. I have bleach under my sink and never had a CAP inspector say anything about it being there. On Thu, Jan 31, 2019 at 8:31 AM Maryann Deathridge via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello Histonetters! > Has anyone heard of a regulation by CAP, CLIA or OSHA regarding commercial > bleach stored under the laboratory sink. My lab routinely has a gallon > container of dated/ bio-labeled commercial bleach stored under the sink for > cleaning purposes. Daily use to clean special stain coplin jars,etc.. I > had a suspicious med tech tell my pathologist that is was against > regulations to have it in the lab anytime ! > HUH???? > Responses welcomed. > Have an awesome Thursday! > > > > Maryann Deathridge, Lab Manager > Pathology Assoc. of St. Thomas > 4220 Harding Pike > Bldg. SE, Suite 504 > Nashville, TN 37205 > madeathridge at pastnashville.com > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From VKurth at uwhealth.org Thu Jan 31 12:22:41 2019 From: VKurth at uwhealth.org (Kurth Virginia L.) Date: Thu, 31 Jan 2019 18:22:41 +0000 Subject: [Histonet] Bleach Regs In-Reply-To: References: , Message-ID: I agree! Ginny Kurth Developmental specialist University Hospital of Wisconsin Surgical Pathology ________________________________ From: Gareth Davis via Histonet Sent: Thursday, January 31, 2019 11:52:24 AM To: Maryann Deathridge Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Bleach Regs WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Hi Maryann, Nosy Med techs. I have bleach under my sink and never had a CAP inspector say anything about it being there. On Thu, Jan 31, 2019 at 8:31 AM Maryann Deathridge via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello Histonetters! > Has anyone heard of a regulation by CAP, CLIA or OSHA regarding commercial > bleach stored under the laboratory sink. My lab routinely has a gallon > container of dated/ bio-labeled commercial bleach stored under the sink for > cleaning purposes. Daily use to clean special stain coplin jars,etc.. I > had a suspicious med tech tell my pathologist that is was against > regulations to have it in the lab anytime ! > HUH???? > Responses welcomed. > Have an awesome Thursday! > > > > Maryann Deathridge, Lab Manager > Pathology Assoc. of St. Thomas > 4220 Harding Pike > Bldg. SE, Suite 504 > Nashville, TN 37205 > madeathridge at pastnashville.com > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegghm at hotmail.com Thu Jan 31 12:51:40 2019 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Thu, 31 Jan 2019 18:51:40 +0000 Subject: [Histonet] guinea pig IHC In-Reply-To: References: Message-ID: In my experience it is not that GP have a higher peroxidase level, it is frozen sections in general that cannot be blocked with h202, unless they are fixed for a long time in formalin. What are others experiences with h202 blocking on frozen sections. I always used an IHC detection system that did not require h202 blocking for frozen sections. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Jan Shivers Sent: Tuesday, January 29, 2019 12:58 PM To: histonet Subject: [Histonet] guinea pig IHC Has anyone ever performed IHC on frozen sections of guinea pig tissue? I am experiencing an enormous amount of bubbling when doing the peroxidase blocking step, even though I'm only using a 0.3% concentration of H2O2. And when I say 'enormous', I mean it's like continuous champagne bubbles rising out of the tissue, even after 20 minutes in the H2O2 solution. I can't find anything in the literature that mentions guinea pigs having a higher peroxidase content in their tissues. Thanks for any help that anyone can provide. Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003 at umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* From tbraud at holyredeemer.com Thu Jan 31 13:03:44 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 31 Jan 2019 19:03:44 +0000 Subject: [Histonet] bleach under the sink Message-ID: <48E053DDF6CE074DB6A7414BA05403F8013BE4F1D3@HRHEX02-HOS.holyredeemer.local> Cleaning supplies may be stored under the sink, but since "things" have a way of migrating there as well, we just have a blanket rule, nothing under the sink. Of course, this would never occur in the Histology Department, LOL. The space is actually locked off. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Histotechnologist Needed near Philly! (Kristyn Ferber) 2. Bleach Regs (Maryann Deathridge) 3. Re: Bleach Regs (Laurie Redmond) 4. Re: Bleach Regs (Morken, Timothy) Message: 2 Date: Thu, 31 Jan 2019 15:11:45 +0000 From: Maryann Deathridge Subject: [Histonet] Bleach Regs Hello Histonetters! Has anyone heard of a regulation by CAP, CLIA or OSHA regarding commercial bleach stored under the laboratory sink. My lab routinely has a gallon container of dated/ bio-labeled commercial bleach stored under the sink for cleaning purposes. Daily use to clean special stain coplin jars,etc.. I had a suspicious med tech tell my pathologist that is was against regulations to have it in the lab anytime ! HUH???? Responses welcomed. Have an awesome Thursday! Maryann Deathridge, Lab Manager Pathology Assoc. of St. Thomas 4220 Harding Pike Bldg. SE, Suite 504 Nashville, TN 37205 madeathridge at pastnashville.com