From Valerie.Hannen at parrishmed.com Fri Feb 1 04:49:40 2019 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Fri, 1 Feb 2019 05:49:40 -0500 Subject: [Histonet] FW: [External Sender] Re: bleach under the sink In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C3243D9ECE03@isexstore03> References: <48E053DDF6CE074DB6A7414BA05403F8013BE4F1D3@HRHEX02-HOS.holyredeemer.local> <450B7A81EDA0C54E97C53D60F00776C3243D9ECE03@isexstore03> Message-ID: <450B7A81EDA0C54E97C53D60F00776C3243D9ECE04@isexstore03> -----Original Message----- From: Hannen, Valerie Sent: Friday, February 01, 2019 5:47 AM To: 'Terri Braud' Subject: RE: [External Sender] Re: [Histonet] bleach under the sink We are not allowed to store anything under our sinks, the Compliance department has told us that it is an OSHA requirement. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Terri Braud via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, January 31, 2019 2:04 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [External Sender] Re: [Histonet] bleach under the sink WARNING: This message came from an external source. Please do not click links or open attachments if unexpected or unusual. Cleaning supplies may be stored under the sink, but since "things" have a way of migrating there as well, we just have a blanket rule, nothing under the sink. Of course, this would never occur in the Histology Department, LOL. The space is actually locked off. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Histotechnologist Needed near Philly! (Kristyn Ferber) 2. Bleach Regs (Maryann Deathridge) 3. Re: Bleach Regs (Laurie Redmond) 4. Re: Bleach Regs (Morken, Timothy) Message: 2 Date: Thu, 31 Jan 2019 15:11:45 +0000 From: Maryann Deathridge Subject: [Histonet] Bleach Regs Hello Histonetters! Has anyone heard of a regulation by CAP, CLIA or OSHA regarding commercial bleach stored under the laboratory sink. My lab routinely has a gallon container of dated/ bio-labeled commercial bleach stored under the sink for cleaning purposes. Daily use to clean special stain coplin jars,etc.. I had a suspicious med tech tell my pathologist that is was against regulations to have it in the lab anytime ! HUH???? Responses welcomed. Have an awesome Thursday! Maryann Deathridge, Lab Manager Pathology Assoc. of St. Thomas 4220 Harding Pike Bldg. SE, Suite 504 Nashville, TN 37205 madeathridge at pastnashville.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From Nancy_Schmitt at pa-ucl.com Fri Feb 1 09:52:18 2019 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Fri, 1 Feb 2019 15:52:18 +0000 Subject: [Histonet] cytology listserv Message-ID: Hello- I know many of us have overlap between histology/cytology/cytology prep. Wondering if there is a helpful listserv available like this - or is my best chance to bring my questions here? Thanks Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From twebster at CRH.org Fri Feb 1 12:28:30 2019 From: twebster at CRH.org (Webster, Thomas S.) Date: Fri, 1 Feb 2019 18:28:30 +0000 Subject: [Histonet] cytology listserv Message-ID: <93fc6a1cc62f41f5ad5890786ab042da@CRH.org> Haven't seen any cytology listservs except the one for members of the ASC. There are some cytology facebook pages where you could get questions answered. This Histonet listserv is very informative. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From pruegghm at hotmail.com Fri Feb 1 12:39:54 2019 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Fri, 1 Feb 2019 18:39:54 +0000 Subject: [Histonet] guinea pig IHC In-Reply-To: References: , Message-ID: Especially a very blood tissue like GP spleen. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Patsy Ruegg Sent: Thursday, January 31, 2019 11:51 AM To: Jan Shivers; histonet Subject: Re: [Histonet] guinea pig IHC In my experience it is not that GP have a higher peroxidase level, it is frozen sections in general that cannot be blocked with h202, unless they are fixed for a long time in formalin. What are others experiences with h202 blocking on frozen sections. I always used an IHC detection system that did not require h202 blocking for frozen sections. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Jan Shivers Sent: Tuesday, January 29, 2019 12:58 PM To: histonet Subject: [Histonet] guinea pig IHC Has anyone ever performed IHC on frozen sections of guinea pig tissue? I am experiencing an enormous amount of bubbling when doing the peroxidase blocking step, even though I'm only using a 0.3% concentration of H2O2. And when I say 'enormous', I mean it's like continuous champagne bubbles rising out of the tissue, even after 20 minutes in the H2O2 solution. I can't find anything in the literature that mentions guinea pigs having a higher peroxidase content in their tissues. Thanks for any help that anyone can provide. Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003 at umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* From carl.hobbs at kcl.ac.uk Sat Feb 2 13:38:05 2019 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sat, 2 Feb 2019 19:38:05 +0000 Subject: [Histonet] Re guinea pig IHC In-Reply-To: References: Message-ID: Ms Ruegg, as usual , gives excellent advice: avoid HRP. Use Alk phos? Block end. alk phos by using levamisole. However, Ms Shivers does not state the fixation status of her FS. Is the Gpig tissue perfused-fixed then frozen or....frozen as unfixed...then fixed? Also....why 0.3% H2O2? Use 3%....kill the enzyme, not feed it? NOT aq for FS Make up in IMS ( 74OP) No tissue disruption However: you state that you get loadsa bubbles...so what? Is your section still attached to your slide? Can you then carry out successful IF/IHC? If yes....no problem. Sure, there's the argument that using a coagulant ( alcohol) in block is a No No I never had a problem....provided that the Formalin fixation was sufficient for unfixed crosections ( 15 mins) I do STILL severely dislike FS ( sure, I spent many years in Diagnostic Histopath doing Operative FS)? ?Why not use Pwax sections, Ms Shivers? Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From: histonet-request at lists.utsouthwestern.edu Sent: 02 February 2019 18:00 To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 183, Issue 2 ? Send Histonet mailing list submissions to ??????? histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??????? https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C1546c2da69de40ae400f08d689398cc4%7C8370cf1416f34c16b83c724071654356%7C0&sdata=gEf%2FzAdfyRdZG0CI%2Bty4tXrOxlk5NwyD4O9qxP8DZ0E%3D&reserved=0 or, via email, send a message with subject or body 'help' to ??????? histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at ??????? histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ?? 1. Re: cytology listserv (Webster, Thomas S.) ?? 2. Re: guinea pig IHC (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Fri, 1 Feb 2019 18:28:30 +0000 From: "Webster, Thomas S." To: "'histonet at lists.utsouthwestern.edu'" ??????? Subject: Re: [Histonet] cytology listserv Message-ID: <93fc6a1cc62f41f5ad5890786ab042da at CRH.org> Content-Type: text/plain; charset="us-ascii" Haven't seen any cytology listservs except the one for members of the ASC.? There are some cytology facebook pages where you could get questions answered. This Histonet listserv is very informative. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information.? If you are not the intended recipient, please contact the sender by reply e-mail immediately.? Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 ------------------------------ Message: 2 Date: Fri, 1 Feb 2019 18:39:54 +0000 From: Patsy Ruegg To: Jan Shivers , histonet ??????? Subject: Re: [Histonet] guinea pig IHC Message-ID: ??????? ??????? Content-Type: text/plain; charset="iso-8859-1" Especially a very blood tissue like GP spleen. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Patsy Ruegg Sent: Thursday, January 31, 2019 11:51 AM To: Jan Shivers; histonet Subject: Re: [Histonet] guinea pig IHC In my experience it is not that GP have a higher peroxidase level, it is frozen sections in general that cannot be blocked with h202, unless they are fixed for a long time in formalin.? What are others experiences with h202 blocking on frozen sections.? I always used an IHC detection system that did not require h202 blocking for frozen sections. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Jan Shivers Sent: Tuesday, January 29, 2019 12:58 PM To: histonet Subject: [Histonet] guinea pig IHC Has anyone ever performed IHC on frozen sections of guinea pig tissue?? I am experiencing an enormous amount of bubbling when doing the peroxidase blocking step, even though I'm only using a 0.3% concentration of H2O2. And when I say 'enormous', I mean it's like continuous champagne bubbles rising out of the tissue, even after 20 minutes in the H2O2 solution. I can't find anything in the literature that mentions guinea pigs having a higher peroxidase content in their tissues. Thanks for any help that anyone can provide. Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN? 55108 612-624-7297 shive003 at umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C1546c2da69de40ae400f08d689398cc4%7C8370cf1416f34c16b83c724071654356%7C0&sdata=gEf%2FzAdfyRdZG0CI%2Bty4tXrOxlk5NwyD4O9qxP8DZ0E%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 183, Issue 2 **************************************** From pruegghm at hotmail.com Sun Feb 3 13:53:57 2019 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Sun, 3 Feb 2019 19:53:57 +0000 Subject: [Histonet] Re guinea pig IHC In-Reply-To: References: , Message-ID: good advise Carl and you asked some of the things I was wondering. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Hobbs, Carl Sent: Saturday, February 2, 2019 12:38 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Re guinea pig IHC Ms Ruegg, as usual , gives excellent advice: avoid HRP. Use Alk phos? Block end. alk phos by using levamisole. However, Ms Shivers does not state the fixation status of her FS. Is the Gpig tissue perfused-fixed then frozen or....frozen as unfixed...then fixed? Also....why 0.3% H2O2? Use 3%....kill the enzyme, not feed it? NOT aq for FS Make up in IMS ( 74OP) No tissue disruption However: you state that you get loadsa bubbles...so what? Is your section still attached to your slide? Can you then carry out successful IF/IHC? If yes....no problem. Sure, there's the argument that using a coagulant ( alcohol) in block is a No No I never had a problem....provided that the Formalin fixation was sufficient for unfixed crosections ( 15 mins) I do STILL severely dislike FS ( sure, I spent many years in Diagnostic Histopath doing Operative FS) Why not use Pwax sections, Ms Shivers? Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 From: histonet-request at lists.utsouthwestern.edu Sent: 02 February 2019 18:00 To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 183, Issue 2 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C1546c2da69de40ae400f08d689398cc4%7C8370cf1416f34c16b83c724071654356%7C0&sdata=gEf%2FzAdfyRdZG0CI%2Bty4tXrOxlk5NwyD4O9qxP8DZ0E%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: cytology listserv (Webster, Thomas S.) 2. Re: guinea pig IHC (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Fri, 1 Feb 2019 18:28:30 +0000 From: "Webster, Thomas S." To: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] cytology listserv Message-ID: <93fc6a1cc62f41f5ad5890786ab042da at CRH.org> Content-Type: text/plain; charset="us-ascii" Haven't seen any cytology listservs except the one for members of the ASC. There are some cytology facebook pages where you could get questions answered. This Histonet listserv is very informative. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 ------------------------------ Message: 2 Date: Fri, 1 Feb 2019 18:39:54 +0000 From: Patsy Ruegg To: Jan Shivers , histonet Subject: Re: [Histonet] guinea pig IHC Message-ID: Content-Type: text/plain; charset="iso-8859-1" Especially a very blood tissue like GP spleen. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Patsy Ruegg Sent: Thursday, January 31, 2019 11:51 AM To: Jan Shivers; histonet Subject: Re: [Histonet] guinea pig IHC In my experience it is not that GP have a higher peroxidase level, it is frozen sections in general that cannot be blocked with h202, unless they are fixed for a long time in formalin. What are others experiences with h202 blocking on frozen sections. I always used an IHC detection system that did not require h202 blocking for frozen sections. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Jan Shivers Sent: Tuesday, January 29, 2019 12:58 PM To: histonet Subject: [Histonet] guinea pig IHC Has anyone ever performed IHC on frozen sections of guinea pig tissue? I am experiencing an enormous amount of bubbling when doing the peroxidase blocking step, even though I'm only using a 0.3% concentration of H2O2. And when I say 'enormous', I mean it's like continuous champagne bubbles rising out of the tissue, even after 20 minutes in the H2O2 solution. I can't find anything in the literature that mentions guinea pigs having a higher peroxidase content in their tissues. Thanks for any help that anyone can provide. Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003 at umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C1546c2da69de40ae400f08d689398cc4%7C8370cf1416f34c16b83c724071654356%7C0&sdata=gEf%2FzAdfyRdZG0CI%2Bty4tXrOxlk5NwyD4O9qxP8DZ0E%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 183, Issue 2 **************************************** From BAMoe at gundersenhealth.org Mon Feb 4 11:35:25 2019 From: BAMoe at gundersenhealth.org (Moe, Barbi A) Date: Mon, 4 Feb 2019 17:35:25 +0000 Subject: [Histonet] Control tissue Message-ID: Could anyone share their process of finding and validating control tissues for IHC? We currently use tonsil tissue as control tissue for 23 different antibodies for IHC (CD2, CD3, CD4, etc.). My current process is to have our grossing personnel submit some extra tissue from a surgical case. I cut an H&E section on the tissue to verify it has fixed properly and is not needed for diagnosis. Once cleared by the pathologist I cut the tissue into smaller sections to create individual control blocks. I then cut a section of each block and test it for each antibody. I can usually mount 6 sections on a slide - so in essence I am checking 6 control blocks at one time. However, since the control block could be used for any of the 23 antibodies, I check each antibody to make sure the tissue is good for all - so 23 slides are being generated for every 6 blocks of control tissue made. Is this overkill? Do I need to check each block that is created? A pathologist here feels that once he checks the H&E section and says the tissue "should be good" that I shouldn't need to run each antibody and generate all those slides. The CAP question ANP.21395 states that control tissue needs to be verified and recorded as acceptable "prior to or concurrent with the reporting of patient results." Does anyone verify and record their control tissue as acceptable "concurrent with the reporting" - and not check it before using it? Any thoughts would be greatly appreciated as it is becoming more and more difficult to obtain/verify/maintain control blocks for as fast as we are using them due to increased workload!! Thanks in advance. Barb Moe Gundersen Health System La Crosse WI bamoe at gundersenhealth.org if you'd like to respond individually From john.garratt at ciqc.ca Mon Feb 4 19:44:19 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Tue, 05 Feb 2019 01:44:19 +0000 Subject: [Histonet] Control tissue In-Reply-To: References: Message-ID: You will first need to document the control tissue you are collecting so you have the ischemic time, fixation time and processing schedule in your records. Make sure you control your fixation time and don't just grab tissue out of wet storage, this will save you a lot of grief. Then give each block a unique identifier that is referenced to the curated tissue. The more blocks you can collect from the original tissue the better. Ultimately your control slides will also carry this identifier so if you want to refer back to the collection process you can do. Then take one of the blocks and stain it with a group of antibodies that will confirm the antigenicity of the tissue you collected. You then only need to test one block from the group of blocked you procured from the original tissue. I recommend you ultimately construct a compound block of tonsil, pancreas, Liver and appendix making sure each tissue is tested before creating the block. Of course the compound block is identified so you can reference back to pedigree of the individual tissue it was constructed with. H&E to ensure there is normal tissue and no evidence of autolysis IHC Testing on each block Appendix ? CD20, Desmin, Calretin Tonsil ? AE1/AE3, CD10, CD58 Pancreas ? CK7, Insulin, CD56 Liver ? HAS, CK19, Muramadase For those with an interest in standardizing controls ( and who doesn't?) I recommend this article "Standardization of positive controls in diagnostic immunohistochemistry: recommendations from the international ad hoc expert committee."?Applied Immunohistochemistry & Molecular Morphology?23, no. 1 (2015): 1-18. https://www.researchgate.net/publication/282488454_Standardization_of_Positive_Controls_in_Diagnostic_Immunohistochemistry Also there is series of 4 publications that are well worth reading. This is the link to Part 4, just to give you a taste. Evolution of Quality Assurance for Clinical Immunohistochemistry in the Era of Precision Medicine:https://www.researchgate.net/publication/311565221_Evolution_of_Quality_Assurance_for_Clinical_Immunohistochemistry_in_the_Era_of_Precision_Medicine_Part_4 www.ciqc.ca ??????? Original Message ??????? On Monday, February 4, 2019 9:35 AM, Moe, Barbi A via Histonet wrote: > Could anyone share their process of finding and validating control tissues for IHC? > > We currently use tonsil tissue as control tissue for 23 different antibodies for IHC (CD2, CD3, CD4, etc.). > > My current process is to have our grossing personnel submit some extra tissue from a surgical case. I cut an H&E section on the tissue to verify it has fixed properly and is not needed for diagnosis. Once cleared by the pathologist I cut the tissue into smaller sections to create individual control blocks. I then cut a section of each block and test it for each antibody. I can usually mount 6 sections on a slide - so in essence I am checking 6 control blocks at one time. > > However, since the control block could be used for any of the 23 antibodies, I check each antibody to make sure the tissue is good for all - so 23 slides are being generated for every 6 blocks of control tissue made. > > Is this overkill? Do I need to check each block that is created? A pathologist here feels that once he checks the H&E section and says the tissue "should be good" that I shouldn't need to run each antibody and generate all those slides. > > The CAP question ANP.21395 states that control tissue needs to be verified and recorded as acceptable "prior to or concurrent with the reporting of patient results." > > Does anyone verify and record their control tissue as acceptable "concurrent with the reporting" - and not check it before using it? > > Any thoughts would be greatly appreciated as it is becoming more and more difficult to obtain/verify/maintain control blocks for as fast as we are using them due to increased workload!! > > Thanks in advance. > > Barb Moe > > Gundersen Health System > > La Crosse WI > > bamoe at gundersenhealth.org if you'd like to respond individually > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford at DignityHealth.org Thu Feb 7 07:19:07 2019 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Thu, 7 Feb 2019 13:19:07 +0000 Subject: [Histonet] ER/PR question Message-ID: <2d9f0469501a45f8bb9905de59663400@PHX-EXCH-013.chw.edu> Good Morning, One of my Pathologists wants me to do some ER/PR's on 9 year old tissue blocks. I know ER and PR can be sensitive are the IHC's going to work or will there be too much antigen decay? Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you From melissa at alliedsearchpartners.com Thu Feb 7 08:28:39 2019 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Thu, 7 Feb 2019 14:28:39 +0000 Subject: [Histonet] Histotech Job in New Hyde Park, NY Area Full Time/Permanent Message-ID: Good Morning, Quick note to spread the word about my Full Time Permanent Histotech job in New Hyde Park, NY area. Please contact me for job description/shift/details. Thank you, Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners Direct Line: 407.413.9117 From rjbuesa at yahoo.com Thu Feb 7 09:22:35 2019 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 7 Feb 2019 15:22:35 +0000 (UTC) Subject: [Histonet] ER/PR question In-Reply-To: <2d9f0469501a45f8bb9905de59663400@PHX-EXCH-013.chw.edu> References: <2d9f0469501a45f8bb9905de59663400@PHX-EXCH-013.chw.edu> Message-ID: <1011043176.4635450.1549552955647@mail.yahoo.com> Time alone is not the only cause for antigen decay for?it is also associated with storage conditions and, as such, you cannot "predict" % decay based on time alone.Just try one block and let the pathologist decide based on the usefulness of what he sees in that try test.Ren? On Thursday, February 7, 2019 8:23 AM, "Heckford, Karen - SMMC-SF via Histonet" wrote: Good Morning, One of my Pathologists wants me to do some ER/PR's on 9 year old tissue blocks.? I know ER and PR can be sensitive are the IHC's going to work or will there be too much antigen decay? Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica.Piche at wtbyhosp.org Thu Feb 7 10:48:40 2019 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Thu, 7 Feb 2019 16:48:40 +0000 Subject: [Histonet] bachelors of science Message-ID: Hello Everyone, Do you need a bachelor's of science to sit for the HTL exam? I thought you had to have a bachelor's degree, and the right amount of credits in science, chemistry, etc. Thanks in advance. Jessica Piche, HT(ASCP) Waterbury Hospital From nelsonrnch at verizon.net Thu Feb 7 10:48:41 2019 From: nelsonrnch at verizon.net (Patti Nelson) Date: Thu, 7 Feb 2019 08:48:41 -0800 Subject: [Histonet] Fwd: VIP 5 References: <1D8A5FC69BF54843B30E5CEFC4FD58FC01C1DC8B@tvdd-3.tvddc.com> Message-ID: <14BF509A-1566-4E3B-8270-1F1F33421D73@verizon.net> Patti Nelson HT (ASCP) 909-841-9761 Sent from my iPhone Begin forwarded message: > From: Patricia Nelson > Date: February 7, 2019 at 8:38:23 AM PST > To: "nelsonrnch at verizon.net" > Subject: FW: VIP 5 > > > From: Patricia Nelson > Sent: Thursday, February 07, 2019 8:37 AM > To: histonet at lists.utsouthwestern.edu > Subject: VIP 5 > > Hi Histo World, > > HELP!! Mid December our VIP 5 went down. I work in a GI lab with a workload of 300 daily. Our lab obtained another refurbished VIP 5. But now we are starting to see the same symptoms as we seen on our old VIP before it went down. Some of the symptoms are formalin carry over (formalin level goes down after each run), our 95 w/eosin is carrying over to all solutions all the way to the paraffin. Now our small GI bx are starting to feel unfixed and has patchy staining. Has anyone dealt with any of these issues? > We maintenance our VIP weekly and change all solutions and all paraffins Once a month we do our warm water flush. Below is our set up > > 1st level: Formalin, Formalin, 70%, 80%, 95% w/eosin, > 2nd level: 100,100, 100, xylene, xylene > 3rd level: cleaning xylene, cleaning alcohol, water, empty, fume con. water > > Patti Nelson (HT ASCP) > United Gastroenterologists > From allanvv at gmail.com Thu Feb 7 11:49:41 2019 From: allanvv at gmail.com (Allan Wang) Date: Thu, 7 Feb 2019 12:49:41 -0500 Subject: [Histonet] ER/PR question In-Reply-To: <2d9f0469501a45f8bb9905de59663400@PHX-EXCH-013.chw.edu> References: <2d9f0469501a45f8bb9905de59663400@PHX-EXCH-013.chw.edu> Message-ID: The antigen doesn't decay much inside the tissue block, as long as the sections were freshly done. We redo ER/PR on archived samples from 15 years ago and they still stain very strongly. Allan On Thu, Feb 7, 2019 at 8:26 AM Heckford, Karen - SMMC-SF via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good Morning, > One of my Pathologists wants me to do some ER/PR's on 9 year old tissue > blocks. I know ER and PR can be sensitive are the IHC's going to work or > will there be too much antigen decay? > > Thanks, > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford at dignityhealth.org > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the > intended recipient, you have received this document in error. Any further > review, dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please > notify us immediately by reply email. Thank you > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From John at imebinc.com Thu Feb 7 12:33:20 2019 From: John at imebinc.com (=?utf-8?Q?John_O=E2=80=99Brien?=) Date: Thu, 7 Feb 2019 11:33:20 -0700 Subject: [Histonet] Histonet Digest, Vol 183, Issue 6 In-Reply-To: References: Message-ID: To whom it concerns My company for 30 years have sold refurbished vip tissue processor The refurbishment was not done properly your ceramics Value must have small cracks in it allowing chemical to creep into other chemicals on process If u bought this vip from a reputable service company it should be under warranty IMEB Mangement John O?Brien > On Feb 7, 2019, at 11:00 AM, histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. ER/PR question (Heckford, Karen - SMMC-SF) > 2. Histotech Job in New Hyde Park, NY Area Full Time/Permanent > (Melissa Owens) > 3. Re: ER/PR question (Rene J Buesa) > 4. bachelors of science (Piche, Jessica) > 5. Fwd: VIP 5 (Patti Nelson) > 6. Re: ER/PR question (Allan Wang) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 7 Feb 2019 13:19:07 +0000 > From: "Heckford, Karen - SMMC-SF" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] ER/PR question > Message-ID: <2d9f0469501a45f8bb9905de59663400 at PHX-EXCH-013.chw.edu> > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > One of my Pathologists wants me to do some ER/PR's on 9 year old tissue blocks. I know ER and PR can be sensitive are the IHC's going to work or will there be too much antigen decay? > > Thanks, > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford at dignityhealth.org > > Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you > > > > ------------------------------ > > Message: 2 > Date: Thu, 7 Feb 2019 14:28:39 +0000 > From: Melissa Owens > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Histotech Job in New Hyde Park, NY Area Full > Time/Permanent > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Good Morning, > > Quick note to spread the word about my Full Time Permanent Histotech job in New Hyde Park, NY area. Please contact me for job description/shift/details. Thank you, > > Melissa Owens, CHP (ASA) > President, Laboratory Staffing > Allied Search Partners > Direct Line: 407.413.9117 > > > > > > ------------------------------ > > Message: 3 > Date: Thu, 7 Feb 2019 15:22:35 +0000 (UTC) > From: Rene J Buesa > To: "Heckford, Karen - SMMC-SF" , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] ER/PR question > Message-ID: <1011043176.4635450.1549552955647 at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > Time alone is not the only cause for antigen decay for?it is also associated with storage conditions and, as such, you cannot "predict" % decay based on time alone.Just try one block and let the pathologist decide based on the usefulness of what he sees in that try test.Ren? > > On Thursday, February 7, 2019 8:23 AM, "Heckford, Karen - SMMC-SF via Histonet" wrote: > > > Good Morning, > One of my Pathologists wants me to do some ER/PR's on 9 year old tissue blocks.? I know ER and PR can be sensitive are the IHC's going to work or will there be too much antigen decay? > > Thanks, > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckford at dignityhealth.org > > Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Thu, 7 Feb 2019 16:48:40 +0000 > From: "Piche, Jessica" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] bachelors of science > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Hello Everyone, > > Do you need a bachelor's of science to sit for the HTL exam? I thought you had to have a bachelor's degree, and the right amount of credits in science, chemistry, etc. > > Thanks in advance. > > Jessica Piche, HT(ASCP) > Waterbury Hospital > > > ------------------------------ > > Message: 5 > Date: Thu, 7 Feb 2019 08:48:41 -0800 > From: Patti Nelson > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Fwd: VIP 5 > Message-ID: <14BF509A-1566-4E3B-8270-1F1F33421D73 at verizon.net> > Content-Type: text/plain; charset=us-ascii > > > > Patti Nelson HT (ASCP) 909-841-9761 Sent from my iPhone > > Begin forwarded message: > >> From: Patricia Nelson >> Date: February 7, 2019 at 8:38:23 AM PST >> To: "nelsonrnch at verizon.net" >> Subject: FW: VIP 5 >> >> >> From: Patricia Nelson >> Sent: Thursday, February 07, 2019 8:37 AM >> To: histonet at lists.utsouthwestern.edu >> Subject: VIP 5 >> >> Hi Histo World, >> >> HELP!! Mid December our VIP 5 went down. I work in a GI lab with a workload of 300 daily. Our lab obtained another refurbished VIP 5. But now we are starting to see the same symptoms as we seen on our old VIP before it went down. Some of the symptoms are formalin carry over (formalin level goes down after each run), our 95 w/eosin is carrying over to all solutions all the way to the paraffin. Now our small GI bx are starting to feel unfixed and has patchy staining. Has anyone dealt with any of these issues? >> We maintenance our VIP weekly and change all solutions and all paraffins Once a month we do our warm water flush. Below is our set up >> >> 1st level: Formalin, Formalin, 70%, 80%, 95% w/eosin, >> 2nd level: 100,100, 100, xylene, xylene >> 3rd level: cleaning xylene, cleaning alcohol, water, empty, fume con. water >> >> Patti Nelson (HT ASCP) >> United Gastroenterologists >> > > > ------------------------------ > > Message: 6 > Date: Thu, 7 Feb 2019 12:49:41 -0500 > From: Allan Wang > To: "Heckford, Karen - SMMC-SF" > Cc: "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] ER/PR question > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > The antigen doesn't decay much inside the tissue block, as long as the > sections were freshly done. We redo ER/PR on archived samples from 15 years > ago and they still stain very strongly. > > Allan > > On Thu, Feb 7, 2019 at 8:26 AM Heckford, Karen - SMMC-SF via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Good Morning, >> One of my Pathologists wants me to do some ER/PR's on 9 year old tissue >> blocks. I know ER and PR can be sensitive are the IHC's going to work or >> will there be too much antigen decay? >> >> Thanks, >> >> Karen Heckford HT ASCP CE >> Lead Histology Technician >> St. Mary's Medical Center >> 450 Stanyan St. >> San Francisco, Ca. 94117 >> 415-668-1000 ext. 6167 >> karen.heckford at dignityhealth.org >> >> Caution: This email message, including all content and attachments, is >> CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The >> information contained in this email message is intended only for the use of >> the recipient(s) named above. If the reader of this message is not the >> intended recipient or an agent responsible for delivering it to the >> intended recipient, you have received this document in error. Any further >> review, dissemination, distribution, or copying of this message is strictly >> prohibited. If you have received this communication in error, please >> notify us immediately by reply email. Thank you >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 183, Issue 6 > **************************************** From rsrichmond at gmail.com Thu Feb 7 12:36:08 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 7 Feb 2019 13:36:08 -0500 Subject: [Histonet] ER/PR question In-Reply-To: References: Message-ID: Karen Heckford HT ASCP CE at St. Mary's Medical Center in San Francisco, CA asks: >>One of my pathologists wants me to do some ER/PR's on 9 year old tissue blocks. I know ER and PR can be sensitive are the IHC's going to work or will there be too much antigen decay?<< You can only try. There should be some antigen positive ducts and what have you in these 9 year old tissues that should serve as an internal control. If there's nothing positive, I'd interpret negative results cautiously. But it's worth a try. Bob Richmond Samurai Pathologist Maryville TN From jonesly at wustl.edu Thu Feb 7 14:00:08 2019 From: jonesly at wustl.edu (Jones, Lynne) Date: Thu, 7 Feb 2019 20:00:08 +0000 Subject: [Histonet] Cut-resistant gloves and SOP for frozen brain In-Reply-To: References: Message-ID: Hello - I would appreciate feedback from the HistoNet community on best practices for handling frozen human (or macaque) brain for autoradiography in a research lab. I'm also curious about lightweight cut resistant gloves, and whether they are practical for anything more than changing blades (if even that). We recently obtained frozen slabs of brain tissue that are larger than the material our group routinely sections. The PI purchased a Mar-Med Bone saw to cut the slabs into smaller blocks we can then section and mount on glass slides. It should be a safe option for biologists with no experience using any power tools. I need to update our EH&S and biosafety protocol for the saw, so I'm also revisiting our BBP/cryostat safety training. The institutional biosafety office suggested lightweight cut-resistant gloves made with ultrahigh molecular weight polyethylene fiber, layered with nitrile. They look like cotton liners, Spec-Tec and Spectra fiber are trade names. Are they useful? If they weren't so expensive (and we didn't need two different sizes), I wouldn't hesitate to try them. We follow standard universal/microbial practices for biohazards, with surgical mask and eye protection for splash hazards when dust/aerosols are a concern. The new Mar-Med saw will go in a fume hood. I've explained the use of push blocks or a jig to hold the tissue when using a band saw (probably have some made from melamine). Any other tips/tricks when using a saw on frozen brain? The company rep suggested ultra-fine blades, but I don't know if diamond would be better for brain. Should brain tissue be cut at -80 degrees or at -20 degrees? Do users chill the saw with ice or dry ice? Cleaning and disinfection for the saw will follow manufacturers SOP for disassembly of the parts and our cryotome disinfection protocol. The cryotome is cleaned and decontaminated at room temperature. Equipment surfaces are sometimes covered with foil to facilitate cleanup. We use lab tissues to clean surfaces and non-removable parts after discarding the blade. (Any foil is also discarded.) Chucks and other parts are removed and soaked in a neutral lab detergent. We clean surfaces with soap, then warm water rinse, then 70% ethanol, then 95% alcohol to remove water. After it is dry, final decontamination is with a waterless EPA-registered spray disinfectant (kills TB); spray it on so that it looks wet for ~2 minutes. 20 minutes after spraying with the EPA-registered disinfected, the cryotome can be set to standard temperature for use. Am I missing anything? We are an academic/research lab, so don't need to follow CAP. Much of our work uses rodent tissue, so there isn't a set schedule for cryotome disinfection - or even cleaning. Lab Specific Training is clean/disinfect both before and after working with potentially infectious material (human or macaque). Thanks, Lynne Jones Research Lab Supervisor Radiochemistry Research Group - Lab Manager Mallinckrodt Institute of Radiology Washington University School of Medicine St Louis, MO CONFIDENTIALITY NOTICE ________________________________________ The materials in this message are private and may contain Protected Healthcare Information or other information of a sensitive nature. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail, and please delete the e-mail without further review, disclosure or copying. ________________________________ The materials in this message are private and may contain Protected Healthcare Information or other information of a sensitive nature. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return mail. From tony.henwood at health.nsw.gov.au Thu Feb 7 18:00:46 2019 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 8 Feb 2019 00:00:46 +0000 Subject: [Histonet] FW: ER/PR question References: <2d9f0469501a45f8bb9905de59663400@PHX-EXCH-013.chw.edu> Message-ID: <14729ae15f934256b131548af25cf738@SVDCMBX-MEX024.nswhealth.net> This paper might be useful: Ehinger, A., Bendahl, P. O., Ryd?n, L., Fern?, M., & Alkner, S. (2018). Stability of oestrogen and progesterone receptor antigenicity in formalin?fixed paraffin?embedded breast cancer tissue over time. Apmis, 126(9), 746-754. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 8 February 2019 12:19 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] ER/PR question Good Morning, One of my Pathologists wants me to do some ER/PR's on 9 year old tissue blocks. I know ER and PR can be sensitive are the IHC's going to work or will there be too much antigen decay? Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From greg.dobbin at gmail.com Fri Feb 8 07:51:59 2019 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Fri, 8 Feb 2019 09:51:59 -0400 Subject: [Histonet] ER/PR question Message-ID: Hi Karen, As mentioned by others "decay" is not likely going to be an issue. More concerning for you could be not knowing how those tissues were handled prior to processing 10 years ago. Presumably, you now track cold ischemic times and have standardized your fixation protocols for breast tissues and you have validated your IHC procedures with these current parameters. If your lab was like many others 10 years ago, these parameters were probably not maintained so rigorously. So interpretation of a negative result may be problematic. Having said that, there is the internal control for both ER and PR. It seems to me that if the internal control is staining adequately, then perhaps interpretation will not be an issue. I am curious, does anyone have a different take on relying the internal control as a guide in this particular situation?? Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From DSiena at statlab.com Fri Feb 8 10:50:40 2019 From: DSiena at statlab.com (Debra Siena) Date: Fri, 8 Feb 2019 16:50:40 +0000 Subject: [Histonet] Derm IHC question Message-ID: Hello fellow Histonetters I would like to ask you a question about IHC staining and derm cases. I am seeing a peculiar issue going on, where the melanocytes in the middle of the tissues are staining pretty well but when you get to the ends of the tissues either shaves or ellipses, they are not staining. This is sporadic, not every case and there is no consensus as to a common thread between the cases. I feel that this may be a fixation issue but was just wondering if anyone had ever seen the same phenomena and would be willing to share the theory or even better what was the remedy behind this issue. The fixative is 10% Neutral Buffered Formaliln and the cells in question that are "dropping out" which is what the pathologist is describing are melanocytes, especially with Sox-10 and Mart 1 antibodies. Thanks for the assistance, I definitely appreciate it very much. Best wishes, [image001] Debbie Siena, HT(ASCP)QIHC Empowering Anatomic Pathology Technical Support Manager, StatLab 2090 Commerce| McKinney, TX 75069 t: 800.442.3573 ext 229 | m: 469-400-6897 | f: 972-436-1369 dsiena at statlab.com|www.statlab.com StatLab is an ISO 13485 Certified Company From Richard.Cartun at hhchealth.org Fri Feb 8 10:59:04 2019 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 8 Feb 2019 16:59:04 +0000 Subject: [Histonet] ER/PR question In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EAC1086F5@HHCEXCHMB03.hhcsystem.org> If the tumor is ER/PR negative the first thing I do is to look for an internal positive control (immunoreactive benign breast epithelium). In my experience, the majority of these cases have internal positive controls to validate the negative ER/PR results. When I don't see internal positive controls, and the HER2 is negative, I recommend that the testing be repeated on excisional tumor for confirmation. This is why it's important to submit adjacent benign breast tissue along with the tumor tissue when grossing. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org -----Original Message----- From: Greg Dobbin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, February 08, 2019 8:52 AM To: karen.heckford at dignityhealth.org; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] ER/PR question CAUTION: This email is from outside HHC. USE CARE when opening attachments or links. Hi Karen, As mentioned by others "decay" is not likely going to be an issue. More concerning for you could be not knowing how those tissues were handled prior to processing 10 years ago. Presumably, you now track cold ischemic times and have standardized your fixation protocols for breast tissues and you have validated your IHC procedures with these current parameters. If your lab was like many others 10 years ago, these parameters were probably not maintained so rigorously. So interpretation of a negative result may be problematic. Having said that, there is the internal control for both ER and PR. It seems to me that if the internal control is staining adequately, then perhaps interpretation will not be an issue. I am curious, does anyone have a different take on relying the internal control as a guide in this particular situation?? Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=6t6lv-HaXrWz9L26Ub8EE5c9k76MJqlTDQ1XNA9cb2Q&s=_Gu4paw-d2iygUvP770iXrMhJFfeQlU9bt3MZLe-NIA&e= Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Richard.Cartun at hhchealth.org Fri Feb 8 11:29:42 2019 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 8 Feb 2019 17:29:42 +0000 Subject: [Histonet] Derm IHC question In-Reply-To: References: Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EAC108732@HHCEXCHMB03.hhcsystem.org> Doesn't sound like a fixation issue to me. Could the tissue be drying out before it's placed in formalin? Also, are these specimens inked for assessment of margins? I've seen ink interfere with immunoreactivity. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org -----Original Message----- From: Debra Siena via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, February 08, 2019 11:51 AM To: 'histonet' Subject: [Histonet] Derm IHC question CAUTION: This email is from outside HHC. USE CARE when opening attachments or links. Hello fellow Histonetters I would like to ask you a question about IHC staining and derm cases. I am seeing a peculiar issue going on, where the melanocytes in the middle of the tissues are staining pretty well but when you get to the ends of the tissues either shaves or ellipses, they are not staining. This is sporadic, not every case and there is no consensus as to a common thread between the cases. I feel that this may be a fixation issue but was just wondering if anyone had ever seen the same phenomena and would be willing to share the theory or even better what was the remedy behind this issue. The fixative is 10% Neutral Buffered Formaliln and the cells in question that are "dropping out" which is what the pathologist is describing are melanocytes, especially with Sox-10 and Mart 1 antibodies. Thanks for the assistance, I definitely appreciate it very much. Best wishes, [image001] Debbie Siena, HT(ASCP)QIHC Empowering Anatomic Pathology Technical Support Manager, StatLab 2090 Commerce| McKinney, TX 75069 t: 800.442.3573 ext 229 | m: 469-400-6897 | f: 972-436-1369 dsiena at statlab.com|https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com&d=DwICAg&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=niLAvgTbNiAqFJIM-oaLdmyfDgppcOQotF3-vo_Qv1M&s=y7Dkipiv1JdLkqba8XolhtugffjOReKOTWuW0mZaJJI&e= StatLab is an ISO 13485 Certified Company _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=niLAvgTbNiAqFJIM-oaLdmyfDgppcOQotF3-vo_Qv1M&s=9GfWQBzHH2U1LIbnBXO5wvUeCTlJulcFAkXN5aWrbCw&e= Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From CDavis at che-east.org Fri Feb 8 13:59:25 2019 From: CDavis at che-east.org (Cassie P. Davis) Date: Fri, 8 Feb 2019 19:59:25 +0000 Subject: [Histonet] possible answer Message-ID: <2debb1fd7b3a417ab36711711c362e09@che-east.org> Reguarding: Hello fellow Histonetters I would like to ask you a question about IHC staining and derm cases. I am seeing a peculiar issue going on, where the melanocytes in the middle of the tissues are staining pretty well but when you get to the ends of the tissues either shaves or ellipses, they are not staining. This is sporadic, not every case and there is no consensus as to a common thread between the cases. I feel that this may be a fixation issue but was just wondering if anyone had ever seen the same phenomena and would be willing to share the theory or even better what was the remedy behind this issue. The fixative is 10% Neutral Buffered Formaliln and the cells in question that are "dropping out" which is what the pathologist is describing are melanocytes, especially with Sox-10 and Mart 1 antibodies. Thanks for the assistance, I definitely appreciate it very much. Best wishes, [image001] Debbie Siena, HT(ASCP)QIHC Empowering Anatomic Pathology Technical Support Manager, StatLab 2090 Commerce| McKinney, TX 75069 t: 800.442.3573 ext 229 | m: 469-400-6897 | f: 972-436-1369 dsiena at statlab.com|www.statlab.com> What you are seeing may be caused by the surgeon waiting before placing the specimen into the formalin. There are several newer studies indicating ischemic time adversely affecting IHC and since the outside of the specimen seems to be showing this, it would seem to fall in-line with the theory. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From CDavis at che-east.org Fri Feb 8 13:59:25 2019 From: CDavis at che-east.org (Cassie P. Davis) Date: Fri, 8 Feb 2019 19:59:25 +0000 Subject: [Histonet] possible answer Message-ID: <2debb1fd7b3a417ab36711711c362e09@che-east.org> Reguarding: Hello fellow Histonetters I would like to ask you a question about IHC staining and derm cases. I am seeing a peculiar issue going on, where the melanocytes in the middle of the tissues are staining pretty well but when you get to the ends of the tissues either shaves or ellipses, they are not staining. This is sporadic, not every case and there is no consensus as to a common thread between the cases. I feel that this may be a fixation issue but was just wondering if anyone had ever seen the same phenomena and would be willing to share the theory or even better what was the remedy behind this issue. The fixative is 10% Neutral Buffered Formaliln and the cells in question that are "dropping out" which is what the pathologist is describing are melanocytes, especially with Sox-10 and Mart 1 antibodies. Thanks for the assistance, I definitely appreciate it very much. Best wishes, [image001] Debbie Siena, HT(ASCP)QIHC Empowering Anatomic Pathology Technical Support Manager, StatLab 2090 Commerce| McKinney, TX 75069 t: 800.442.3573 ext 229 | m: 469-400-6897 | f: 972-436-1369 dsiena at statlab.com|www.statlab.com> What you are seeing may be caused by the surgeon waiting before placing the specimen into the formalin. There are several newer studies indicating ischemic time adversely affecting IHC and since the outside of the specimen seems to be showing this, it would seem to fall in-line with the theory. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From tony.henwood at health.nsw.gov.au Sun Feb 10 16:49:09 2019 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun, 10 Feb 2019 22:49:09 +0000 Subject: [Histonet] Derm IHC question In-Reply-To: References: Message-ID: Possibly, the edges have been allowed to dry prior to immersion in fixative. Also is there evidence of cautery artefact? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Debra Siena via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Saturday, 9 February 2019 3:51 AM To: 'histonet' Subject: [Histonet] Derm IHC question Hello fellow Histonetters I would like to ask you a question about IHC staining and derm cases. I am seeing a peculiar issue going on, where the melanocytes in the middle of the tissues are staining pretty well but when you get to the ends of the tissues either shaves or ellipses, they are not staining. This is sporadic, not every case and there is no consensus as to a common thread between the cases. I feel that this may be a fixation issue but was just wondering if anyone had ever seen the same phenomena and would be willing to share the theory or even better what was the remedy behind this issue. The fixative is 10% Neutral Buffered Formaliln and the cells in question that are "dropping out" which is what the pathologist is describing are melanocytes, especially with Sox-10 and Mart 1 antibodies. Thanks for the assistance, I definitely appreciate it very much. Best wishes, [image001] Debbie Siena, HT(ASCP)QIHC Empowering Anatomic Pathology Technical Support Manager, StatLab 2090 Commerce| McKinney, TX 75069 t: 800.442.3573 ext 229 | m: 469-400-6897 | f: 972-436-1369 dsiena at statlab.com|www.statlab.com StatLab is an ISO 13485 Certified Company _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From lmarie08 at uga.edu Mon Feb 11 14:03:03 2019 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Mon, 11 Feb 2019 20:03:03 +0000 Subject: [Histonet] re-processing help Message-ID: Hello Histonet, We have issues- please advise! Long story short- some tissues did not get processed over the weekend, they went through the process but were not submerged in ANY (we think) of the reagents. Now they are shriveled up and brittle (they are intestinal). Is there any way to save them? Can we re-process them? Has anyone ever had this problem and had success recovering? Gratitude, Lauren From sharon.harrison at uwimona.edu.jm Mon Feb 11 14:58:59 2019 From: sharon.harrison at uwimona.edu.jm (HARRISON,Sharon) Date: Mon, 11 Feb 2019 15:58:59 -0500 Subject: [Histonet] re-processing help In-Reply-To: References: Message-ID: <39D05A5FD7C1334DA749CCFCE8538F87AFF2AD6D08@xchg1.uwimona.edu.jm> Dear Lauren, Please leave samples in formalin for about 48 hours. Some tissues will be recovered others may be a bit more difficult to recover. Then reprocess after the soak and see what happens. Regards. Sharon Harrison Chief Medical Technologist in charge of Histopathology UWI Mona, Dept of Pathology ________________________________________ From: Lauren Sweeney via Histonet [histonet at lists.utsouthwestern.edu] Sent: Monday, February 11, 2019 3:03 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] re-processing help Hello Histonet, We have issues- please advise! Long story short- some tissues did not get processed over the weekend, they went through the process but were not submerged in ANY (we think) of the reagents. Now they are shriveled up and brittle (they are intestinal). Is there any way to save them? Can we re-process them? Has anyone ever had this problem and had success recovering? Gratitude, Lauren _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan at uwo.ca Tue Feb 12 00:12:01 2019 From: jkiernan at uwo.ca (John Kiernan) Date: Tue, 12 Feb 2019 06:12:01 +0000 Subject: [Histonet] Words, phrases and names in histotechnology. A free glossary. Message-ID: Hello histonetters. I'm a HistoNet old-timer, back again after a few years away. It's good to see that a few names are still around from the 1990s. Here is something new that may interest all of us. I send it as a news item; a change from the usual initial question that initiates a Histonet topic. A freely accessible online glossary of words, phrases and eponyms used in histotechnology, histochemistry and immunostaining was published by the Biological Stain Commission (BSC) at the end of December 2018. It includes about 600 entries; it is to be revised and expanded from time to time. (A minor revision was uploaded on 19th January 2019.) Notable features are extensive crosslinks between the entries, and explanations of terminology related to chemical and physical mechanisms involved in the methods. There are also definitions and explanations relating to all the stains (dyes) certified by the BSC. The BSC glossary is readable on screens of all sizes (including mobile phones), and navigation among links is extremely rapid. Check it out directly at https://biologicalstaincommission.org/bscglossary.html. Alternatively, see it in the broader context of the BSC: http://biostain.com. John Kiernan = = = From relia1 at earthlink.net Tue Feb 12 10:33:01 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 12 Feb 2019 11:33:01 -0500 Subject: [Histonet] Relia Histology Opportunities Update 2-12-2019 Happy Valentines' Day. Exciting opportunities, Sweets for the Sweet and a RELIA EXCLUSIVE!!. Message-ID: <000a01d4c2f0$9ff4ff00$dfdefd00$@earthlink.net> Hi Histonetters, I hope this is an especially sweet week since Thursday is Valentines? Day. Let me be the first to wish you: Happy Valentines? Day!!! Happy ?Gal?entine?s Day And Happy ?Pal?entine?s Day!! I also have some great histology opportunities to tell you about. Please feel free to take a second and peruse the list kind of the way one peruses an Assortment of chocolates in a heart shaped box! My personal favorites are Chocolate Covered Cherries!! (Especially the ones that are 50% off on Friday! LOL) How about you? All of these are permanent full time positions and our clients offer excellent compensation, benefits, relocation and or sign on bonuses. Here is a list of my current openings!! Let me know if anything looks good!! SPOTLIGHT OPPORTUNITY! A RELIA EXCLUSIVE!!!!! Senior Histotech ? Days Norfolk, VA $9000.00 Sign on bonus! Management: Histology Territory Sales Manager ? Mid East Region AP Manager ? Milwaukee, WI HT/HTL Opportunities: Histotechnician: Dayshift? Norfolk, VA 9000.00 sign-on bonus Sr. Histotech: Dayshift Norfolk, VA 9000.00 sign-on bonus Dermpath Histotech: Dayshift Birmingham, AL Histology Tech: Dayshift Annapolis, MD Dermpath histotech: Dayshift Springfield, MA Dermpath histotech: Dayshift Milwaukee, WI Grossing Histotech: Nights Chattanooga, TN NY Lic histotech: NYC,NY Histonetters, If you are interested in any of these positions please call or text me on my cell at 407-353-5070 or toll free at 866-607-3542 or e-mail me at relia1 at earthlink.net ? If you would like you can e-mail me your resume and a number where I can reach you at a time that is convenient for you. ? If you are interested in looking into new job opportunities in other areas that are not mentioned above please contact me as well. ? I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember It never hurts to keep an eye open EVEN IF you are happy in your present job. Have a wonderful Valentine?s Day!! Thanks-Pam Thank you, Pam ? 866-607-3542 (866-60RELIA) 407-353-5070-cell ? Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia #jobs4myhistopeeps From rsrichmond at gmail.com Wed Feb 13 16:34:38 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Wed, 13 Feb 2019 17:34:38 -0500 Subject: [Histonet] Words, phrases and names in histotechnology. A free glossary. In-Reply-To: References: Message-ID: John Kiernan, certainly glad to see you back on HistoNet! - At 80 I've finally retired. This Biological Stain Commission glossary is a really useful resource I wish we'd had long ago. A lot of information in it difficult to get elsewhere. I'll link it on Facebook and on Sermo. Two suggestions: BSC should post it in PDF form, since a lot of people will probably want to print it out. And somebody should go through it and put the umlauts and accent marks on all those European names! Bob Richmond Samurai Pathologist Maryville TN ********************************************* > Hello histonetters. I'm a HistoNet old-timer, back again after a few years > away. It's good to see that a few names are still around from the 1990s. > > Here is something new that may interest all of us. I send it as a news > item; a change from the usual initial question that initiates a Histonet > topic. > > A freely accessible online glossary of words, phrases and eponyms used in > histotechnology, histochemistry and immunostaining was published by the > Biological Stain Commission (BSC) at the end of December 2018. It includes > about 600 entries; it is to be revised and expanded from time to time. (A > minor revision was uploaded on 19th January 2019.) > > Notable features are extensive crosslinks between the entries, and > explanations of terminology related to chemical and physical mechanisms > involved in the methods. There are also definitions and explanations > relating to all the stains (dyes) certified by the BSC. > > The BSC glossary is readable on screens of all sizes (including mobile > phones), and navigation among links is extremely rapid. > > Check it out directly at > https://biologicalstaincommission.org/bscglossary.html. Alternatively, > see it in the broader context of the BSC: http://biostain.com. > > John Kiernan > From cforster at umn.edu Wed Feb 13 19:48:40 2019 From: cforster at umn.edu (Colleen Forster) Date: Wed, 13 Feb 2019 19:48:40 -0600 Subject: [Histonet] Words, phrases and names in histotechnology. A free glossary. In-Reply-To: References: Message-ID: THis looks likje a great resource...I will take time to look it over. Thank you for all your valuable mentorship over the years.... Respectfully, Colleen Forster U of MN On Wed, Feb 13, 2019 at 4:35 PM Bob Richmond via Histonet < histonet at lists.utsouthwestern.edu> wrote: > John Kiernan, certainly glad to see you back on HistoNet! - At 80 I've > finally retired. > > This Biological Stain Commission glossary is a really useful resource I > wish we'd had long ago. A lot of information in it difficult to get > elsewhere. I'll link it on Facebook and on Sermo. > > Two suggestions: BSC should post it in PDF form, since a lot of people will > probably want to print it out. And somebody should go through it and put > the umlauts and accent marks on all those European names! > > Bob Richmond > Samurai Pathologist > Maryville TN > ********************************************* > > > Hello histonetters. I'm a HistoNet old-timer, back again after a few > years > > away. It's good to see that a few names are still around from the 1990s. > > > > Here is something new that may interest all of us. I send it as a news > > item; a change from the usual initial question that initiates a Histonet > > topic. > > > > A freely accessible online glossary of words, phrases and eponyms used in > > histotechnology, histochemistry and immunostaining was published by the > > Biological Stain Commission (BSC) at the end of December 2018. It > includes > > about 600 entries; it is to be revised and expanded from time to time. (A > > minor revision was uploaded on 19th January 2019.) > > > > Notable features are extensive crosslinks between the entries, and > > explanations of terminology related to chemical and physical mechanisms > > involved in the methods. There are also definitions and explanations > > relating to all the stains (dyes) certified by the BSC. > > > > The BSC glossary is readable on screens of all sizes (including mobile > > phones), and navigation among links is extremely rapid. > > > > Check it out directly at > > https://biologicalstaincommission.org/bscglossary.html. Alternatively, > > see it in the broader context of the BSC: http://biostain.com. > > > > > > John Kiernan > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at holml at umn.edu * From rsrichmond at gmail.com Wed Feb 13 21:14:38 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Wed, 13 Feb 2019 22:14:38 -0500 Subject: [Histonet] Words, phrases and names in histotechnology. A free glossary. In-Reply-To: References: Message-ID: You're welcome! I sat down and read through most of it as soon as I saw it. So glad John Kiernan's back! On Wed, Feb 13, 2019 at 8:48 PM Colleen Forster wrote: > THis looks likje a great resource...I will take time to look it over. > > Thank you for all your valuable mentorship over the years.... > > Respectfully, > > Colleen Forster > U of MN > > On Wed, Feb 13, 2019 at 4:35 PM Bob Richmond via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> John Kiernan, certainly glad to see you back on HistoNet! - At 80 I've >> finally retired. >> >> This Biological Stain Commission glossary is a really useful resource I >> wish we'd had long ago. A lot of information in it difficult to get >> elsewhere. I'll link it on Facebook and on Sermo. >> >> Two suggestions: BSC should post it in PDF form, since a lot of people >> will >> probably want to print it out. And somebody should go through it and put >> the umlauts and accent marks on all those European names! >> >> Bob Richmond >> Samurai Pathologist >> Maryville TN >> ********************************************* >> >> > Hello histonetters. I'm a HistoNet old-timer, back again after a few >> years >> > away. It's good to see that a few names are still around from the 1990s. >> > >> > Here is something new that may interest all of us. I send it as a news >> > item; a change from the usual initial question that initiates a Histonet >> > topic. >> > >> > A freely accessible online glossary of words, phrases and eponyms used >> in >> > histotechnology, histochemistry and immunostaining was published by the >> > Biological Stain Commission (BSC) at the end of December 2018. It >> includes >> > about 600 entries; it is to be revised and expanded from time to time. >> (A >> > minor revision was uploaded on 19th January 2019.) >> > >> > Notable features are extensive crosslinks between the entries, and >> > explanations of terminology related to chemical and physical mechanisms >> > involved in the methods. There are also definitions and explanations >> > relating to all the stains (dyes) certified by the BSC. >> > >> > The BSC glossary is readable on screens of all sizes (including mobile >> > phones), and navigation among links is extremely rapid. >> > >> > Check it out directly at >> > https://biologicalstaincommission.org/bscglossary.html. Alternatively, >> > see it in the broader context of the BSC: http://biostain.com. >> > >> >> >> > John Kiernan >> > >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > -- > Colleen Forster HT(ASCP)QIHC > BLS Histology and IHC Laboratory > B173 PWB 612-626-1930 > > *If submitting histology request please also forward to Lori Holm at > holml at umn.edu * > > > From cforster at umn.edu Wed Feb 13 21:51:41 2019 From: cforster at umn.edu (Colleen Forster) Date: Wed, 13 Feb 2019 21:51:41 -0600 Subject: [Histonet] Words, phrases and names in histotechnology. A free glossary. In-Reply-To: References: Message-ID: Yes! Your expertise is such a valuable resource.. C On Wednesday, February 13, 2019, Bob Richmond wrote: > You're welcome! > > I sat down and read through most of it as soon as I saw it. So glad John > Kiernan's back! > > > On Wed, Feb 13, 2019 at 8:48 PM Colleen Forster wrote: > >> THis looks likje a great resource...I will take time to look it over. >> >> Thank you for all your valuable mentorship over the years.... >> >> Respectfully, >> >> Colleen Forster >> U of MN >> >> On Wed, Feb 13, 2019 at 4:35 PM Bob Richmond via Histonet < >> histonet at lists.utsouthwestern.edu> wrote: >> >>> John Kiernan, certainly glad to see you back on HistoNet! - At 80 I've >>> finally retired. >>> >>> This Biological Stain Commission glossary is a really useful resource I >>> wish we'd had long ago. A lot of information in it difficult to get >>> elsewhere. I'll link it on Facebook and on Sermo. >>> >>> Two suggestions: BSC should post it in PDF form, since a lot of people >>> will >>> probably want to print it out. And somebody should go through it and put >>> the umlauts and accent marks on all those European names! >>> >>> Bob Richmond >>> Samurai Pathologist >>> Maryville TN >>> ********************************************* >>> >>> > Hello histonetters. I'm a HistoNet old-timer, back again after a few >>> years >>> > away. It's good to see that a few names are still around from the >>> 1990s. >>> > >>> > Here is something new that may interest all of us. I send it as a news >>> > item; a change from the usual initial question that initiates a >>> Histonet >>> > topic. >>> > >>> > A freely accessible online glossary of words, phrases and eponyms used >>> in >>> > histotechnology, histochemistry and immunostaining was published by the >>> > Biological Stain Commission (BSC) at the end of December 2018. It >>> includes >>> > about 600 entries; it is to be revised and expanded from time to time. >>> (A >>> > minor revision was uploaded on 19th January 2019.) >>> > >>> > Notable features are extensive crosslinks between the entries, and >>> > explanations of terminology related to chemical and physical mechanisms >>> > involved in the methods. There are also definitions and explanations >>> > relating to all the stains (dyes) certified by the BSC. >>> > >>> > The BSC glossary is readable on screens of all sizes (including mobile >>> > phones), and navigation among links is extremely rapid. >>> > >>> > Check it out directly at >>> > https://biologicalstaincommission.org/bscglossary.html. Alternatively, >>> > see it in the broader context of the BSC: http://biostain.com. >>> > >>> >>> >>> > John Kiernan >>> > >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> -- >> Colleen Forster HT(ASCP)QIHC >> BLS Histology and IHC Laboratory >> B173 PWB 612-626-1930 >> >> *If submitting histology request please also forward to Lori Holm at >> holml at umn.edu * >> >> >> -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at holml at umn.edu * From cmcgrad1 at hurleymc.com Thu Feb 14 07:17:41 2019 From: cmcgrad1 at hurleymc.com (Cynthia McGrady) Date: Thu, 14 Feb 2019 08:17:41 -0500 Subject: [Histonet] Xylene free processing Message-ID: Our laboratory is experimenting with xylene free processing on our Sakura VIP. , substituting Isopropanol for our xylene substitute. Our Pathologists have complained about processing, especially the biopsies, with our regular processing, they have the washed out look, nuclear staining is faint. If you are using the Xylene free processing, can you provide feedback, good or bad, any suggestions for optimal processing, etc... Thank you in advance for your help.. CindyMcGrady -- If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. From mcruz84pr at gmail.com Thu Feb 14 08:01:52 2019 From: mcruz84pr at gmail.com (Maria Cruz) Date: Thu, 14 Feb 2019 09:01:52 -0500 Subject: [Histonet] QIHC Exam prep course Message-ID: All: For those of you who are not members of the NSH I thought you should know that this organization has recently announced a new program on preparing for the QIHC. I?ve taken this course by the same speaker at a state conference and it was excellent. I don?t think it could have passed the exam without taking it. More information is available on NSH web site. Maria From rsrichmond at gmail.com Thu Feb 14 12:43:36 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 14 Feb 2019 13:43:36 -0500 Subject: [Histonet] Xylene free processing In-Reply-To: References: Message-ID: Cindy McGrady at Hurley Medical Center in Flint, Michigan asks: >>Our laboratory is experimenting with xylene free processing on our Sakura VIP, substituting isopropanol for our xylene substitute. Our pathologists have complained about processing, especially the biopsies, with our regular processing, they have the washed out look, nuclear staining is faint. If you are using the xylene free processing, can you provide feedback, good or bad, any suggestions for optimal processing, etc?<< If the isopropanol isn't working, you need to go back to your xylene substitute, or if it didn't work, to a different aliphatic xylene substitute - they aren't all alike (and if you're going to recover them by distillation, you'll need to stick with a single brand). - I suppose few people are processing with limonene (smells like citrus fruit) any more. Bob Richmond Samurai Pathologist Maryville TN From Timothy.Morken at ucsf.edu Fri Feb 15 10:57:48 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 15 Feb 2019 16:57:48 +0000 Subject: [Histonet] Histotechnologist I-II opening at UC San Francisco Message-ID: Histotechnologist I-II opening at University of California, San Francisco Medical Center, San Francisco, CA Job ID 17907 See posting at: https://careers.ucsfmedicalcenter.org:8443/psp/hcmprd_cg/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_APP_SCHJOB.GBL?Page=HRS_APP_JBPST&Action=U&FOCUS=Applicant&SiteId=1&JobOpeningId=17907&PostingSeq=2 Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From CIngles at uwhealth.org Fri Feb 15 15:24:01 2019 From: CIngles at uwhealth.org (Ingles Claire) Date: Fri, 15 Feb 2019 21:24:01 +0000 Subject: [Histonet] ST4020 Stainer In-Reply-To: References: Message-ID: Netters! A question on behalf of a co-worker. We are in the process of validating the ST4020 stainer from Leica. We are a Mohs clinic that currently uses Carazzi's double strength Hematoxylin. We are looking for a better staining protocol. We are planning on moving to a Harris formula when switching so any Heme formula is possible right now. Claire From nguy0515 at gmail.com Sat Feb 16 14:36:38 2019 From: nguy0515 at gmail.com (Trini Nguyen) Date: Sat, 16 Feb 2019 12:36:38 -0800 Subject: [Histonet] ST4020 Stainer (Ingles Claire) In-Reply-To: References: Message-ID: We like using Richard Allen products. Eosin Y Alcoholic, hematoxylin 2 and their clarifier On Sat, Feb 16, 2019 at 10:04 AM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. ST4020 Stainer (Ingles Claire) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 15 Feb 2019 21:24:01 +0000 > From: Ingles Claire > To: Histonet > Subject: [Histonet] ST4020 Stainer > Message-ID: > < > DM5PR17MB124139962667A811D3577AB7C9600 at DM5PR17MB1241.namprd17.prod.outlook.com > > > > Content-Type: text/plain; charset="iso-8859-1" > > > > > Netters! > A question on behalf of a co-worker. We are in the process of validating > the ST4020 stainer from Leica. We are a Mohs clinic that currently uses > Carazzi's double strength Hematoxylin. We are looking for a better staining > protocol. We are planning on moving to a Harris formula when switching so > any Heme formula is possible right now. > Claire > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 183, Issue 13 > ***************************************** > From mward at wakehealth.edu Mon Feb 18 14:37:53 2019 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Mon, 18 Feb 2019 20:37:53 +0000 Subject: [Histonet] Vendors closed on Presidents Day Message-ID: Am I the only one that finds it odd and frustrating that some vendors are closed on Presidents Day? I just tried to call the technical service number for Dako about a problem and the message said to call back tomorrow! Hospital labs don't close and I would have thought they would at least have someone staffing the number to cover issues that come up. Thankfully my call isn't urgent but it could have been and then I would be stuck. Martha Ward Wake Forest Baptist Medical Center From Richard.Cartun at hhchealth.org Mon Feb 18 14:57:19 2019 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 18 Feb 2019 20:57:19 +0000 Subject: [Histonet] Question for Leica Bond IHC Users Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EAC10D23B@HHCEXCHMB03.hhcsystem.org> We are preparing to install a Leica "Bond Stain Interface" which will allow us to move away from having to print slide labels manually for our IHC slides. I am not an expert on this so I am looking for help. We have "5" Bond IHC instruments that will be hooked-up to the controller; however, we have been told that we cannot connect our 6th instrument unless we get a second controller (and that's not going to happen). Has anyone else been in a similar predicament? And, if so, how have you resolved it? We need to use all "6" instruments. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From julio.benavides at csic.es Tue Feb 19 02:59:53 2019 From: julio.benavides at csic.es (=?UTF-8?Q?Julio_Benavides_Silv=c3=a1n?=) Date: Tue, 19 Feb 2019 09:59:53 +0100 Subject: [Histonet] comparison between tissue processors In-Reply-To: References: Message-ID: <95882da2-93c1-f8a3-af77-bd5db92dfb1a@csic.es> Hello everybody, we are buying a new tissue processor for the lab and, at the moment, we are considering these three models: Thermo Excelsior AS Leica HistoCore PEARL SLL MTM I/II I would very much appreciate your thoughts/experience regarding any of these models. We are a small research lab, so, normally, no huge burden of blocks. Thanks a lot for your help Regards Julio Benavides Silv?n Instituto de Ganader?a de Monta?a (CSIC-Universidad de Le?n) 24346. Grulleros, Le?n +34 987317156 ext.861162 From gu.lang at gmx.at Tue Feb 19 10:53:50 2019 From: gu.lang at gmx.at (Gudrun Lang) Date: Tue, 19 Feb 2019 17:53:50 +0100 Subject: [Histonet] FISH question Message-ID: <000001d4c873$b0b6ef00$1224cd00$@gmx.at> Dear histonetters! I have difficulties with my FISH preparation on FFPET. I struggle with massive background. It looks like a thick fluorescent film. The signals can't be seen because of the background. Even the nuclei are hard to see. The background is within the tissue but also surrounds it. Therefore it must be directly on the glass slide. The slide is clear after deparaffination and after pretreatment with citric buffer and pepsin. After the pepsin the slides are rinsed in 2xSSC, then 50%-70%-96%-100% ethanol (p.a.). And then the slides are airdried. On the dry slides foggy streams appear. The slides become turbid. When I rinse them again in graded ethanols it becomes better but still a little turbid. After hybridisation and stringent washing the slides are air-dried again and coverslipped with Dapi. When looking at the slides in the fluorescence microscope the trouble arises. My assumption is, that there is a remnant of the salt of the SSC buffer. How can I inhibit this deposit? Can I replace the buffer with water without any harm to the tissue? Or ist there a different cause for the turbidiy? I use fresh reagenses from xylene to buffer and ethanol. Any hints are welcome. Thanks in advance Gudrun Lang From relia1 at earthlink.net Tue Feb 19 11:07:59 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 19 Feb 2019 12:07:59 -0500 Subject: [Histonet] Best Interview Question EVER!! How would YOU answer it? Message-ID: <000001d4c875$aac5b700$00512500$@earthlink.net> Dear Histonetters, I hope you are having a great week! Coming soon to an interview near you Have you seen Facebook?s Million Dollar interview question? Here it is ?On your very best day at work - the day you come home and think you have the best job in the world -what did you do that day?? Facebook uses this question to find out what their candidates are passionate about. If you were asked this question how would you answer it? If your answer doesn?t match up with a day At your current position We NEED to talk! I can help you find the position that you are passionate about, the place where every day is your best day at work. I have many histotechs that I have worked with over the years that can answer that question with the enthusiasm it deserves because I helped them find that job. Let me help you too! Histonetters, If you are looking for a job today, tomorrow, in 3 months, 6 months, a year, 5 years. I am here for you. My best day at work is every day! Since my passion is to find the right opportunity for you! Here is a list of my current opportunities: VA Senior Histology Tech VA Histotechnician AL Dermpath Histotech OH Territory Sales Manager MA Dermpath Histotech MA Histotechnician (32 hours) MD Histotechnician CA Grossing Histotech TN Grossing Histotech NY Histotechnician (NY lic. Req.) All of these opportunities are full time permanent positions with some of the leading employers nationwide. There are 1st, 2nd and 3rd shift positions open. Experienced and entry level and ASCP or eligible!! My clients offer excellent compensation, benefits and relocation/sign on bonuses. And they can?t wait to speak to you!!! Please contact me at relia1 at earthlink.net or toll free at 866-607-3542 or call/text me on my cell at 407-353-5070 if you are interested in more information on any of these positions or if you would like for me to work on a custom job search for you. Thanks-Pam Right Place, Right Time, Right Move with RELIA! #jobs4myhistopeeps Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From naira.margaryan at hsc.wvu.edu Fri Feb 8 10:51:07 2019 From: naira.margaryan at hsc.wvu.edu (Margaryan, Naira) Date: Fri, 8 Feb 2019 16:51:07 +0000 Subject: [Histonet] Coverslipper Message-ID: Happy Thursday, We would like to sell our slightly used but in excellent condition Dako Coverslipper which was purchased in 2010 just for $10K (purchase price was $ 25K). The instrument can handle up to 600 slides per hour making it one of the fastest on the market. In addition to the flexibility of the Coverslipper it is easy and straightforward to operate and cleaning and maintenance is simple to do. It is small enough to fit into fume cabinets, easy to move around and accepts a variety of commercial mounting media. Will take any offer, Naira From marktarango at gmail.com Tue Feb 19 11:42:42 2019 From: marktarango at gmail.com (Mark Tarango) Date: Tue, 19 Feb 2019 09:42:42 -0800 Subject: [Histonet] FISH question In-Reply-To: <000001d4c873$b0b6ef00$1224cd00$@gmx.at> References: <000001d4c873$b0b6ef00$1224cd00$@gmx.at> Message-ID: Hi Gudrun, Are you sure you have digested long enough with pepsin? If the tissue is not well digested you will see background. We use sodium thiocyanate for pretreatment reagent, not citric buffer. These are my first thoughts. Mark On Tue, Feb 19, 2019 at 9:18 AM Gudrun Lang via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Dear histonetters! > > I have difficulties with my FISH preparation on FFPET. I struggle with > massive background. It looks like a thick fluorescent film. > > The signals can't be seen because of the background. Even the nuclei are > hard to see. > > The background is within the tissue but also surrounds it. Therefore it > must > be directly on the glass slide. > > > > The slide is clear after deparaffination and after pretreatment with citric > buffer and pepsin. After the pepsin the slides are rinsed in 2xSSC, then > 50%-70%-96%-100% ethanol (p.a.). > > And then the slides are airdried. > > On the dry slides foggy streams appear. The slides become turbid. When I > rinse them again in graded ethanols it becomes better but still a little > turbid. > > After hybridisation and stringent washing the slides are air-dried again > and > coverslipped with Dapi. > > When looking at the slides in the fluorescence microscope the trouble > arises. > > > > My assumption is, that there is a remnant of the salt of the SSC buffer. > How > can I inhibit this deposit? Can I replace the buffer with water without any > harm to the tissue? > > Or ist there a different cause for the turbidiy? > > I use fresh reagenses from xylene to buffer and ethanol. > > Any hints are welcome. > > > > Thanks in advance > > Gudrun Lang > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang at gmx.at Tue Feb 19 12:26:10 2019 From: gu.lang at gmx.at (Gudrun Lang) Date: Tue, 19 Feb 2019 19:26:10 +0100 Subject: [Histonet] FISH question In-Reply-To: <67d8eaa7163346dbb0da3f57fa661368@SVR-EXCH13-VP02.OSUMC.EDU> References: <000001d4c873$b0b6ef00$1224cd00$@gmx.at> <67d8eaa7163346dbb0da3f57fa661368@SVR-EXCH13-VP02.OSUMC.EDU> Message-ID: <000301d4c880$98a7f460$c9f7dd20$@gmx.at> Thanks for your advice. I use adhesive slides from Leica. Years ago we had Superfrosts of another brand (maybe Thermo) and I can't remember similar issues. Can you recommend a special brand for FISH? Have you ever tried to do FISH on a slide without adhesion? Gudrun -----Urspr?ngliche Nachricht----- Von: Whitaker, Bonnie [mailto:Bonnie.Whitaker at osumc.edu] Gesendet: Dienstag, 19. Februar 2019 18:54 An: 'Mark Tarango'; Gudrun Lang Betreff: RE: [Histonet] FISH question I know a few years ago, we ran into the same issue, and the problem actually was with the slides. We were using cheaper slides for most of histology at the time, but had to purchase "higher end" slides for the FISH. Thanks, Bonnie Bonnie P. Whitaker AP Operations Director The Ohio State University Wexner Medical Center Department of Pathology N305 Doan Hall 410 West 10th Avenue Columbus, Ohio? 43210 614.293.8418 FAX 614.293.2779 Pager: 614.293.7243 ext. 5013 -----Original Message----- From: Mark Tarango via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, February 19, 2019 12:43 PM To: Gudrun Lang Cc: HistoNet Subject: Re: [Histonet] FISH question Hi Gudrun, Are you sure you have digested long enough with pepsin? If the tissue is not well digested you will see background. We use sodium thiocyanate for pretreatment reagent, not citric buffer. These are my first thoughts. Mark On Tue, Feb 19, 2019 at 9:18 AM Gudrun Lang via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Dear histonetters! > > I have difficulties with my FISH preparation on FFPET. I struggle with > massive background. It looks like a thick fluorescent film. > > The signals can't be seen because of the background. Even the nuclei are > hard to see. > > The background is within the tissue but also surrounds it. Therefore it > must > be directly on the glass slide. > > > > The slide is clear after deparaffination and after pretreatment with citric > buffer and pepsin. After the pepsin the slides are rinsed in 2xSSC, then > 50%-70%-96%-100% ethanol (p.a.). > > And then the slides are airdried. > > On the dry slides foggy streams appear. The slides become turbid. When I > rinse them again in graded ethanols it becomes better but still a little > turbid. > > After hybridisation and stringent washing the slides are air-dried again > and > coverslipped with Dapi. > > When looking at the slides in the fluorescence microscope the trouble > arises. > > > > My assumption is, that there is a remnant of the salt of the SSC buffer. > How > can I inhibit this deposit? Can I replace the buffer with water without any > harm to the tissue? > > Or ist there a different cause for the turbidiy? > > I use fresh reagenses from xylene to buffer and ethanol. > > Any hints are welcome. > > > > Thanks in advance > > Gudrun Lang > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu _mailman_listinfo_histonet&d=DwICAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFE kBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=gzHjMQcM0W22Saad2E8Pgv a_UOfvxrD1xD0IQ57lDUY&s=rVkT1YjvFe8uXhvIGPusCI6h-qfQm2I-v86i1XIRePc&e= > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu _mailman_listinfo_histonet&d=DwICAg&c=k9MF1d71ITtkuJx-PdWme51dKbmfPEvxwt8SFE kBfs4&r=BgjUe6oLZB0OAcW6Y6Rn-n0Q03Ac4dWC2x8Sg24AeiY&m=gzHjMQcM0W22Saad2E8Pgv a_UOfvxrD1xD0IQ57lDUY&s=rVkT1YjvFe8uXhvIGPusCI6h-qfQm2I-v86i1XIRePc&e= From lmarie08 at uga.edu Tue Feb 19 16:17:09 2019 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Tue, 19 Feb 2019 22:17:09 +0000 Subject: [Histonet] job opening Message-ID: Hello Histonet, There is a job opening for a lab manager position at the University of Georgia. This is a small research lab in the College of Veterinary Medicine. There would be 2 techs, including yourself, and 2 pathologists. Since this is a state government job, the salary does not compete with private industry. However, there are many benefits to working at a state government post, including TRS Teachers Retirement (pension- based retirement) and free tuition at any Georgia State college, including UGA. If interested, apply here: https://www.ugajobsearch.com/postings/59337 Best, Lauren From yesyes at comcast.net Wed Feb 20 11:57:23 2019 From: yesyes at comcast.net (MARY ANN) Date: Wed, 20 Feb 2019 17:57:23 -0000 Subject: [Histonet] (no subject) Message-ID: <4196884.1.1550685436003@localhost> Cindy I can message you about it Sent from Xfinity Connect Application -----Original Message----- From: histonet at lists.utsouthwestern.edu To: histonet at lists.utsouthwestern.edu Sent: 2018-10-02 8:31:45 AM Subject: [Histonet] (no subject) Can anyone out in histo land give me feedback on the IHC instrument by StatLab Quantum HDX and how it compares to other automated platforms. Thanks in advance! Cindy Bird Anatomical Medical Laboratories, Inc. 1600 Scripture Street Denton, TX 76201 940-384-6210 940-384-6000 Fax 940-565-9588 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting at geisinger.edu Thu Feb 21 05:20:59 2019 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Thu, 21 Feb 2019 11:20:59 +0000 Subject: [Histonet] NOTCH2 help Message-ID: Is anyone running NOTCH2 on Bond III OR Benchmark Ultra? Can I take a peek at your protocol and antibody selection? Sent from my iPhone IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From Stefano.Mantero at humanitasresearch.it Thu Feb 21 08:08:26 2019 From: Stefano.Mantero at humanitasresearch.it (MANTERO Stefano RIC) Date: Thu, 21 Feb 2019 14:08:26 +0000 Subject: [Histonet] Bone PMMA sections Message-ID: <144455abc19d4579b3e31092f8c1fdc4@exmbx01.techosp.it> Good morning Histonetters! I'm starting to cut out of bone samples included in PMMA. I would like some suggestion how to storage the slides cut. Many thanks in advance Stefano Dott. Stefano Mantero Human Genome Laboratory CNR-IRGB c/o Humanitas Research Hospital Via Rita Levi Montalcini (Ex Via Dainese) 20090 Pieve Emanuele (MI) Ph. +39 02 8224 5164 (desk) Ph. +39 02 8224 5177 (lab) Fax +39 02 8224 5191 Nota di riservatezza. Il presente messaggio, corredato dei relativi allegati, contiene informazioni da considerarsi strettamente riservate, ed ? destinato esclusivamente al destinatario sopra indicato, il quale ? l'unico autorizzato ad usarlo, copiarlo e, sotto la propria responsabilit?, diffonderlo. Chiunque ricevesse questo messaggio per errore o comunque lo leggesse senza esserne legittimato ? avvertito che trattenerlo, copiarlo, divulgarlo, distribuirlo a persone diverse dal destinatario ? severamente proibito, ed ? pregato di rinviarlo immediatamente al mittente distruggendone l'originale. Grazie Confidentiality Notice. This message, together with its annexes, contains information to be deemed strictly confidential and is destined only to the addressee(s) identified above who only may use, copy and, under his/their responsibility, further disseminate it. If anyone received this message by mistake or reads it without entitlement is forewarned that keeping, copying, disseminating or distributing this message to persons other than the addressee(s) is strictly forbidden and is asked to transmit it immediately to the sender and to erase the original message received. Thank You From melissa at alliedsearchpartners.com Thu Feb 21 10:20:31 2019 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Thu, 21 Feb 2019 16:20:31 +0000 Subject: [Histonet] New York Qualifications for Histology Supervisor Message-ID: Hello, I have a question about the requirements to be a supervisor in New York. New York dept. of Ed states qualifications for a Medical Technologist/Laboratory Supervisor and a Cytotechnologist/Cytology Supervisor but no where does it mention qualifications for a Histototech/Histology Supervisor. So therefore, I have heard that in New York, a Histology Supervisor must qualify under the Medical Technologist/Laboratory Supervisor qualifications statues. This seems impossible to me as this would have to be an individual who becomes a Medical Technologist and by random transfers into a histology role then becomes a supervisor. Does anyone out there in New York legally qualify your histology supervisors who have a bachelors degree but were not former Medical Technologists? Thank you for any help as I have intensely scoped the New York department of Education website in my frustrations. By the way, I am asking this because I have a Day Shift 7:30am-4pm Monday-Friday Histology Supervisor position in the New York Metropolitan area with these requirements and I don't know if there is a way around them. Thank you! Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners From aperl at cmmedical.com Thu Feb 21 10:51:37 2019 From: aperl at cmmedical.com (Perl , Alison) Date: Thu, 21 Feb 2019 16:51:37 +0000 Subject: [Histonet] New York Qualifications for Histology Supervisor In-Reply-To: References: Message-ID: <30760d2c6a13405089350ea89b6d4a48@MK-EXMB02.mkmg.com> Hi Melissa This is true, and a source of my neverending frustration with NYS Office of Professions. Stephanie Shulman (stephanie.shulman at health.ny.gov) is a good person at NYS to get clarification, but she will reiterate the same. NYS has explained to me that "histotechs don't exercise judgment" and thus are not qualified to take on the role of supervisor, even for a histology-only laboratory. The supervisors here in my lab and myself all have Clinical Lab Technologist licenses, from when they were grandfathered back in 2007. I have techs who went to school specifically for Histology, but can never become supervisors. I don't know what anyone will do for the next generation of supervisors - your situation is a real fear. Anyone in NYS who would like to raise this issue with your representatives, I encourage you to do so!! Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at cmmedical.com -----Original Message----- From: Melissa Owens via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 21, 2019 11:21 AM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] New York Qualifications for Histology Supervisor Hello, I have a question about the requirements to be a supervisor in New York. New York dept. of Ed states qualifications for a Medical Technologist/Laboratory Supervisor and a Cytotechnologist/Cytology Supervisor but no where does it mention qualifications for a Histototech/Histology Supervisor. So therefore, I have heard that in New York, a Histology Supervisor must qualify under the Medical Technologist/Laboratory Supervisor qualifications statues. This seems impossible to me as this would have to be an individual who becomes a Medical Technologist and by random transfers into a histology role then becomes a supervisor. Does anyone out there in New York legally qualify your histology supervisors who have a bachelors degree but were not former Medical Technologists? Thank you for any help as I have intensely scoped the New York department of Education website in my frustrations. By the way, I am asking this because I have a Day Shift 7:30am-4pm Monday-Friday Histology Supervisor position in the New York Metropolitan area with these requirements and I don't know if there is a way around them. Thank you! Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and its attachments, if any, may contain confidential or proprietary information and are intended solely for authorized use by the intended recipient(s) only. Any other use of this email is prohibited. If you have received this email in error, you are hereby notified that any retention, disclosure, copying, forwarding, distribution (in whole or in part and whether electronically, written and/or orally) and/or taking of any action in reliance on this email, its contents and/or any attachments thereto is strictly prohibited. If you received this email in error, please notify the sender by replying to this message and permanently delete this email, and any attachments thereto, from your system immediately. From cforster at umn.edu Thu Feb 21 10:58:56 2019 From: cforster at umn.edu (Colleen Forster) Date: Thu, 21 Feb 2019 10:58:56 -0600 Subject: [Histonet] New York Qualifications for Histology Supervisor In-Reply-To: <30760d2c6a13405089350ea89b6d4a48@MK-EXMB02.mkmg.com> References: <30760d2c6a13405089350ea89b6d4a48@MK-EXMB02.mkmg.com> Message-ID: Wow...this is just crazy~ The regulations leave histology in a real tough spot... Feeling for all of you and yes, for the future histology techs coming up~ Colleen Forster U of MN On Thu, Feb 21, 2019 at 10:52 AM Perl , Alison via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Melissa > This is true, and a source of my neverending frustration with NYS Office > of Professions. Stephanie Shulman (stephanie.shulman at health.ny.gov) is a > good person at NYS to get clarification, but she will reiterate the same. > > NYS has explained to me that "histotechs don't exercise judgment" and thus > are not qualified to take on the role of supervisor, even for a > histology-only laboratory. The supervisors here in my lab and myself all > have Clinical Lab Technologist licenses, from when they were grandfathered > back in 2007. I have techs who went to school specifically for Histology, > but can never become supervisors. I don't know what anyone will do for the > next generation of supervisors - your situation is a real fear. > > Anyone in NYS who would like to raise this issue with your > representatives, I encourage you to do so!! > > Alison Perl, HTL(ASCP)CM > Anatomic Pathology Manager > CareMount Medical > (914) 302-8424 > aperl at cmmedical.com > > > -----Original Message----- > From: Melissa Owens via Histonet [mailto:histonet at lists.utsouthwestern.edu] > > Sent: Thursday, February 21, 2019 11:21 AM > To: histonet at lists.utsouthwestern.edu > Subject: [EXTERNAL] [Histonet] New York Qualifications for Histology > Supervisor > > Hello, > > I have a question about the requirements to be a supervisor in New York. > New York dept. of Ed states qualifications for a Medical > Technologist/Laboratory Supervisor and a Cytotechnologist/Cytology > Supervisor but no where does it mention qualifications for a > Histototech/Histology Supervisor. So therefore, I have heard that in New > York, a Histology Supervisor must qualify under the Medical > Technologist/Laboratory Supervisor qualifications statues. This seems > impossible to me as this would have to be an individual who becomes a > Medical Technologist and by random transfers into a histology role then > becomes a supervisor. Does anyone out there in New York legally qualify > your histology supervisors who have a bachelors degree but were not former > Medical Technologists? Thank you for any help as I have intensely scoped > the New York department of Education website in my frustrations. > > By the way, I am asking this because I have a Day Shift 7:30am-4pm > Monday-Friday Histology Supervisor position in the New York Metropolitan > area with these requirements and I don't know if there is a way around > them. Thank you! > > Melissa Owens, CHP (ASA) > President, Laboratory Staffing > Allied Search Partners > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This email and its attachments, if any, may contain confidential or > proprietary information and are intended solely for authorized use by the > intended recipient(s) only. Any other use of this email is prohibited. If > you have received this email in error, you are hereby notified that any > retention, disclosure, copying, forwarding, distribution (in whole or in > part and whether electronically, written and/or orally) and/or taking of > any action in reliance on this email, its contents and/or any attachments > thereto is strictly prohibited. If you received this email in error, please > notify the sender by replying to this message and permanently delete this > email, and any attachments thereto, from your system immediately. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at holml at umn.edu * From aperl at cmmedical.com Thu Feb 21 11:07:05 2019 From: aperl at cmmedical.com (Perl , Alison) Date: Thu, 21 Feb 2019 17:07:05 +0000 Subject: [Histonet] [EXTERNAL] Re: New York Qualifications for Histology Supervisor In-Reply-To: References: <30760d2c6a13405089350ea89b6d4a48@MK-EXMB02.mkmg.com> Message-ID: Fortunately our company has tuition reimbursement, so one of my techs is going to school for Med Tech part time (despite her degree in Histology) so that she has a future in this industry. The others are not so ambitious at the moment ? it?s a lot to work FT and got to school 2-3 nights a week! Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager (914) 302-8424 aperl at cmmedical.com From: Colleen Forster [mailto:cforster at umn.edu] Sent: Thursday, February 21, 2019 11:59 AM To: Perl , Alison Cc: Melissa Owens; histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] New York Qualifications for Histology Supervisor Wow...this is just crazy~ The regulations leave histology in a real tough spot... Feeling for all of you and yes, for the future histology techs coming up~ Colleen Forster U of MN On Thu, Feb 21, 2019 at 10:52 AM Perl , Alison via Histonet > wrote: Hi Melissa This is true, and a source of my neverending frustration with NYS Office of Professions. Stephanie Shulman (stephanie.shulman at health.ny.gov) is a good person at NYS to get clarification, but she will reiterate the same. NYS has explained to me that "histotechs don't exercise judgment" and thus are not qualified to take on the role of supervisor, even for a histology-only laboratory. The supervisors here in my lab and myself all have Clinical Lab Technologist licenses, from when they were grandfathered back in 2007. I have techs who went to school specifically for Histology, but can never become supervisors. I don't know what anyone will do for the next generation of supervisors - your situation is a real fear. Anyone in NYS who would like to raise this issue with your representatives, I encourage you to do so!! Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at cmmedical.com -----Original Message----- From: Melissa Owens via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 21, 2019 11:21 AM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] New York Qualifications for Histology Supervisor Hello, I have a question about the requirements to be a supervisor in New York. New York dept. of Ed states qualifications for a Medical Technologist/Laboratory Supervisor and a Cytotechnologist/Cytology Supervisor but no where does it mention qualifications for a Histototech/Histology Supervisor. So therefore, I have heard that in New York, a Histology Supervisor must qualify under the Medical Technologist/Laboratory Supervisor qualifications statues. This seems impossible to me as this would have to be an individual who becomes a Medical Technologist and by random transfers into a histology role then becomes a supervisor. Does anyone out there in New York legally qualify your histology supervisors who have a bachelors degree but were not former Medical Technologists? Thank you for any help as I have intensely scoped the New York department of Education website in my frustrations. By the way, I am asking this because I have a Day Shift 7:30am-4pm Monday-Friday Histology Supervisor position in the New York Metropolitan area with these requirements and I don't know if there is a way around them. Thank you! Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and its attachments, if any, may contain confidential or proprietary information and are intended solely for authorized use by the intended recipient(s) only. Any other use of this email is prohibited. If you have received this email in error, you are hereby notified that any retention, disclosure, copying, forwarding, distribution (in whole or in part and whether electronically, written and/or orally) and/or taking of any action in reliance on this email, its contents and/or any attachments thereto is strictly prohibited. If you received this email in error, please notify the sender by replying to this message and permanently delete this email, and any attachments thereto, from your system immediately. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 If submitting histology request please also forward to Lori Holm at holml at umn.edu This email and its attachments, if any, may contain confidential or proprietary information and are intended solely for authorized use by the intended recipient(s) only. Any other use of this email is prohibited. If you have received this email in error, you are hereby notified that any retention, disclosure, copying, forwarding, distribution (in whole or in part and whether electronically, written and/or orally) and/or taking of any action in reliance on this email, its contents and/or any attachments thereto is strictly prohibited. If you received this email in error, please notify the sender by replying to this message and permanently delete this email, and any attachments thereto, from your system immediately. From KGoodkowsky at goodwin.edu Thu Feb 21 11:11:15 2019 From: KGoodkowsky at goodwin.edu (Kelli Goodkowsky) Date: Thu, 21 Feb 2019 17:11:15 +0000 Subject: [Histonet] New York Qualifications for Histology Supervisor In-Reply-To: <30760d2c6a13405089350ea89b6d4a48@MK-EXMB02.mkmg.com> References: <30760d2c6a13405089350ea89b6d4a48@MK-EXMB02.mkmg.com> Message-ID: This is good information, Alison. Thank you for sharing it. Our Histology Program is in CT and I have many students from the southern part of the state, making NY licensing a viable option for them. It would be interesting to note how NY is defining ?judgment? when it comes to the role of histotechnicians/histotechnologists. I sit on the Educator?s Task force for the NSH and will bring this to their attention as well. Kelli Goodkowsky, M.Ed., HT (ASCP) Program Director, Histologic Science President, Faculty Senate Goodwin College (860) 727-6917 kgoodkowsky at goodwin.edu http://www.goodwin.edu On Feb 21, 2019, at 11:51 AM, Perl , Alison via Histonet > wrote: Hi Melissa This is true, and a source of my neverending frustration with NYS Office of Professions. Stephanie Shulman (stephanie.shulman at health.ny.gov) is a good person at NYS to get clarification, but she will reiterate the same. NYS has explained to me that "histotechs don't exercise judgment" and thus are not qualified to take on the role of supervisor, even for a histology-only laboratory. The supervisors here in my lab and myself all have Clinical Lab Technologist licenses, from when they were grandfathered back in 2007. I have techs who went to school specifically for Histology, but can never become supervisors. I don't know what anyone will do for the next generation of supervisors - your situation is a real fear. Anyone in NYS who would like to raise this issue with your representatives, I encourage you to do so!! Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at cmmedical.com -----Original Message----- From: Melissa Owens via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 21, 2019 11:21 AM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] New York Qualifications for Histology Supervisor Hello, I have a question about the requirements to be a supervisor in New York. New York dept. of Ed states qualifications for a Medical Technologist/Laboratory Supervisor and a Cytotechnologist/Cytology Supervisor but no where does it mention qualifications for a Histototech/Histology Supervisor. So therefore, I have heard that in New York, a Histology Supervisor must qualify under the Medical Technologist/Laboratory Supervisor qualifications statues. This seems impossible to me as this would have to be an individual who becomes a Medical Technologist and by random transfers into a histology role then becomes a supervisor. Does anyone out there in New York legally qualify your histology supervisors who have a bachelors degree but were not former Medical Technologists? Thank you for any help as I have intensely scoped the New York department of Education website in my frustrations. By the way, I am asking this because I have a Day Shift 7:30am-4pm Monday-Friday Histology Supervisor position in the New York Metropolitan area with these requirements and I don't know if there is a way around them. Thank you! Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and its attachments, if any, may contain confidential or proprietary information and are intended solely for authorized use by the intended recipient(s) only. Any other use of this email is prohibited. If you have received this email in error, you are hereby notified that any retention, disclosure, copying, forwarding, distribution (in whole or in part and whether electronically, written and/or orally) and/or taking of any action in reliance on this email, its contents and/or any attachments thereto is strictly prohibited. If you received this email in error, please notify the sender by replying to this message and permanently delete this email, and any attachments thereto, from your system immediately. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster at umn.edu Thu Feb 21 11:12:59 2019 From: cforster at umn.edu (Colleen Forster) Date: Thu, 21 Feb 2019 11:12:59 -0600 Subject: [Histonet] [EXTERNAL] Re: New York Qualifications for Histology Supervisor In-Reply-To: References: <30760d2c6a13405089350ea89b6d4a48@MK-EXMB02.mkmg.com> Message-ID: YEs, it is....I have done it and it is a heavy load...and in this case completely unnecessary....she is qualified without having to be a Med Tech...goodness.... C On Thu, Feb 21, 2019 at 11:07 AM Perl , Alison wrote: > Fortunately our company has tuition reimbursement, so one of my techs is > going to school for Med Tech part time (despite her degree in Histology) so > that she has a future in this industry. The others are not so ambitious at > the moment ? it?s a lot to work FT and got to school 2-3 nights a week! > > > > *Alison Perl, HTL(ASCP)CM* > * Anatomic Pathology Manager * > > (914) 302-8424 > *aperl at cmmedical.com* > > > > *From:* Colleen Forster [mailto:cforster at umn.edu] > *Sent:* Thursday, February 21, 2019 11:59 AM > *To:* Perl , Alison > *Cc:* Melissa Owens; histonet at lists.utsouthwestern.edu > *Subject:* [EXTERNAL] Re: [Histonet] New York Qualifications for > Histology Supervisor > > > > Wow...this is just crazy~ The regulations leave histology in a real tough > spot... > > > > Feeling for all of you and yes, for the future histology techs coming up~ > > > > Colleen Forster > > U of MN > > > > On Thu, Feb 21, 2019 at 10:52 AM Perl , Alison via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > Hi Melissa > This is true, and a source of my neverending frustration with NYS Office > of Professions. Stephanie Shulman (stephanie.shulman at health.ny.gov) is a > good person at NYS to get clarification, but she will reiterate the same. > > NYS has explained to me that "histotechs don't exercise judgment" and thus > are not qualified to take on the role of supervisor, even for a > histology-only laboratory. The supervisors here in my lab and myself all > have Clinical Lab Technologist licenses, from when they were grandfathered > back in 2007. I have techs who went to school specifically for Histology, > but can never become supervisors. I don't know what anyone will do for the > next generation of supervisors - your situation is a real fear. > > Anyone in NYS who would like to raise this issue with your > representatives, I encourage you to do so!! > > Alison Perl, HTL(ASCP)CM > Anatomic Pathology Manager > CareMount Medical > (914) 302-8424 > aperl at cmmedical.com > > > -----Original Message----- > From: Melissa Owens via Histonet [mailto:histonet at lists.utsouthwestern.edu] > > Sent: Thursday, February 21, 2019 11:21 AM > To: histonet at lists.utsouthwestern.edu > Subject: [EXTERNAL] [Histonet] New York Qualifications for Histology > Supervisor > > Hello, > > I have a question about the requirements to be a supervisor in New York. > New York dept. of Ed states qualifications for a Medical > Technologist/Laboratory Supervisor and a Cytotechnologist/Cytology > Supervisor but no where does it mention qualifications for a > Histototech/Histology Supervisor. So therefore, I have heard that in New > York, a Histology Supervisor must qualify under the Medical > Technologist/Laboratory Supervisor qualifications statues. This seems > impossible to me as this would have to be an individual who becomes a > Medical Technologist and by random transfers into a histology role then > becomes a supervisor. Does anyone out there in New York legally qualify > your histology supervisors who have a bachelors degree but were not former > Medical Technologists? Thank you for any help as I have intensely scoped > the New York department of Education website in my frustrations. > > By the way, I am asking this because I have a Day Shift 7:30am-4pm > Monday-Friday Histology Supervisor position in the New York Metropolitan > area with these requirements and I don't know if there is a way around > them. Thank you! > > Melissa Owens, CHP (ASA) > President, Laboratory Staffing > Allied Search Partners > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This email and its attachments, if any, may contain confidential or > proprietary information and are intended solely for authorized use by the > intended recipient(s) only. Any other use of this email is prohibited. If > you have received this email in error, you are hereby notified that any > retention, disclosure, copying, forwarding, distribution (in whole or in > part and whether electronically, written and/or orally) and/or taking of > any action in reliance on this email, its contents and/or any attachments > thereto is strictly prohibited. If you received this email in error, please > notify the sender by replying to this message and permanently delete this > email, and any attachments thereto, from your system immediately. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > > Colleen Forster HT(ASCP)QIHC > > BLS Histology and IHC Laboratory > > B173 PWB 612-626-1930 > > > > *If submitting histology request please also forward to Lori Holm at > holml at umn.edu * > > > > > > This email and its attachments, if any, may contain confidential or > proprietary information and are intended solely for authorized use by the > intended recipient(s) only. Any other use of this email is prohibited. If > you have received this email in error, you are hereby notified that any > retention, disclosure, copying, forwarding, distribution (in whole or in > part and whether electronically, written and/or orally) and/or taking of > any action in reliance on this email, its contents and/or any attachments > thereto is strictly prohibited. If you received this email in error, please > notify the sender by replying to this message and permanently delete this > email, and any attachments thereto, from your system immediately. > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at holml at umn.edu * From patpxs at gmail.com Thu Feb 21 11:18:47 2019 From: patpxs at gmail.com (Patpxs) Date: Thu, 21 Feb 2019 09:18:47 -0800 Subject: [Histonet] New York Qualifications for Histology Supervisor In-Reply-To: References: <30760d2c6a13405089350ea89b6d4a48@MK-EXMB02.mkmg.com> Message-ID: This is an issue that continues to pop up. It all starts with the CLIA regulations that do not recognize histology professionals as equal to other laboratory professionals. Until the feds change CLIA we will keep having this problem. I do believe that NSH is petitioning on our behalf to change the CLIA regulations. In California the state does not recognize Histotechs, certified or not, so we can?t get licensed and we can?t become lab managers as a result. All thanks to CLIA. That?s my rant for the day. Paula Sent from my iPhone > On Feb 21, 2019, at 8:58 AM, Colleen Forster via Histonet wrote: > > Wow...this is just crazy~ The regulations leave histology in a real tough > spot... > > Feeling for all of you and yes, for the future histology techs coming up~ > > Colleen Forster > U of MN > > On Thu, Feb 21, 2019 at 10:52 AM Perl , Alison via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Hi Melissa >> This is true, and a source of my neverending frustration with NYS Office >> of Professions. Stephanie Shulman (stephanie.shulman at health.ny.gov) is a >> good person at NYS to get clarification, but she will reiterate the same. >> >> NYS has explained to me that "histotechs don't exercise judgment" and thus >> are not qualified to take on the role of supervisor, even for a >> histology-only laboratory. The supervisors here in my lab and myself all >> have Clinical Lab Technologist licenses, from when they were grandfathered >> back in 2007. I have techs who went to school specifically for Histology, >> but can never become supervisors. I don't know what anyone will do for the >> next generation of supervisors - your situation is a real fear. >> >> Anyone in NYS who would like to raise this issue with your >> representatives, I encourage you to do so!! >> >> Alison Perl, HTL(ASCP)CM >> Anatomic Pathology Manager >> CareMount Medical >> (914) 302-8424 >> aperl at cmmedical.com >> >> >> -----Original Message----- >> From: Melissa Owens via Histonet [mailto:histonet at lists.utsouthwestern.edu] >> >> Sent: Thursday, February 21, 2019 11:21 AM >> To: histonet at lists.utsouthwestern.edu >> Subject: [EXTERNAL] [Histonet] New York Qualifications for Histology >> Supervisor >> >> Hello, >> >> I have a question about the requirements to be a supervisor in New York. >> New York dept. of Ed states qualifications for a Medical >> Technologist/Laboratory Supervisor and a Cytotechnologist/Cytology >> Supervisor but no where does it mention qualifications for a >> Histototech/Histology Supervisor. So therefore, I have heard that in New >> York, a Histology Supervisor must qualify under the Medical >> Technologist/Laboratory Supervisor qualifications statues. This seems >> impossible to me as this would have to be an individual who becomes a >> Medical Technologist and by random transfers into a histology role then >> becomes a supervisor. Does anyone out there in New York legally qualify >> your histology supervisors who have a bachelors degree but were not former >> Medical Technologists? Thank you for any help as I have intensely scoped >> the New York department of Education website in my frustrations. >> >> By the way, I am asking this because I have a Day Shift 7:30am-4pm >> Monday-Friday Histology Supervisor position in the New York Metropolitan >> area with these requirements and I don't know if there is a way around >> them. Thank you! >> >> Melissa Owens, CHP (ASA) >> President, Laboratory Staffing >> Allied Search Partners >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> This email and its attachments, if any, may contain confidential or >> proprietary information and are intended solely for authorized use by the >> intended recipient(s) only. Any other use of this email is prohibited. If >> you have received this email in error, you are hereby notified that any >> retention, disclosure, copying, forwarding, distribution (in whole or in >> part and whether electronically, written and/or orally) and/or taking of >> any action in reliance on this email, its contents and/or any attachments >> thereto is strictly prohibited. If you received this email in error, please >> notify the sender by replying to this message and permanently delete this >> email, and any attachments thereto, from your system immediately. >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > -- > Colleen Forster HT(ASCP)QIHC > BLS Histology and IHC Laboratory > B173 PWB 612-626-1930 > > *If submitting histology request please also forward to Lori Holm at > holml at umn.edu * > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa at alliedsearchpartners.com Thu Feb 21 11:26:03 2019 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Thu, 21 Feb 2019 17:26:03 +0000 Subject: [Histonet] New York Qualifications for Histology Supervisor In-Reply-To: <30760d2c6a13405089350ea89b6d4a48@MK-EXMB02.mkmg.com> References: <30760d2c6a13405089350ea89b6d4a48@MK-EXMB02.mkmg.com> Message-ID: Wow, thank you for the confirmation. Perhaps the NY Histotechnology Society could help with raising the issue to state representatives, maybe they already have??? This seems odd they would differentiate Cytology from Medical Technology and not Histology from Medical Technology. Thanks again! This is a big problem. I'd love to connect.?Here's my calendar link?to make finding time easy. 2018 Forbes 100 Best Professional Recruitment Firms in America and the only Laboratory Staffing Specific Firm on List! https://www.forbes.com/best-professional-recruiting-firms/list/ Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners LinkedIn:?http://www.linkedin.com/in/melissafaeowens http://www.alliedsearchpartners.com T: 888.388.7571 ext. 102 Direct Line: ?407.413.9117 F: 888.388.7572 -----Original Message----- From: Perl , Alison Sent: Thursday, February 21, 2019 11:52 AM To: Melissa Owens Cc: histonet at lists.utsouthwestern.edu Subject: RE: New York Qualifications for Histology Supervisor Hi Melissa This is true, and a source of my neverending frustration with NYS Office of Professions. Stephanie Shulman (stephanie.shulman at health.ny.gov) is a good person at NYS to get clarification, but she will reiterate the same. NYS has explained to me that "histotechs don't exercise judgment" and thus are not qualified to take on the role of supervisor, even for a histology-only laboratory. The supervisors here in my lab and myself all have Clinical Lab Technologist licenses, from when they were grandfathered back in 2007. I have techs who went to school specifically for Histology, but can never become supervisors. I don't know what anyone will do for the next generation of supervisors - your situation is a real fear. Anyone in NYS who would like to raise this issue with your representatives, I encourage you to do so!! Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at cmmedical.com -----Original Message----- From: Melissa Owens via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 21, 2019 11:21 AM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] New York Qualifications for Histology Supervisor Hello, I have a question about the requirements to be a supervisor in New York. New York dept. of Ed states qualifications for a Medical Technologist/Laboratory Supervisor and a Cytotechnologist/Cytology Supervisor but no where does it mention qualifications for a Histototech/Histology Supervisor. So therefore, I have heard that in New York, a Histology Supervisor must qualify under the Medical Technologist/Laboratory Supervisor qualifications statues. This seems impossible to me as this would have to be an individual who becomes a Medical Technologist and by random transfers into a histology role then becomes a supervisor. Does anyone out there in New York legally qualify your histology supervisors who have a bachelors degree but were not former Medical Technologists? Thank you for any help as I have intensely scoped the New York department of Education website in my frustrations. By the way, I am asking this because I have a Day Shift 7:30am-4pm Monday-Friday Histology Supervisor position in the New York Metropolitan area with these requirements and I don't know if there is a way around them. Thank you! Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and its attachments, if any, may contain confidential or proprietary information and are intended solely for authorized use by the intended recipient(s) only. Any other use of this email is prohibited. If you have received this email in error, you are hereby notified that any retention, disclosure, copying, forwarding, distribution (in whole or in part and whether electronically, written and/or orally) and/or taking of any action in reliance on this email, its contents and/or any attachments thereto is strictly prohibited. If you received this email in error, please notify the sender by replying to this message and permanently delete this email, and any attachments thereto, from your system immediately. From aperl at cmmedical.com Thu Feb 21 11:46:25 2019 From: aperl at cmmedical.com (Perl , Alison) Date: Thu, 21 Feb 2019 17:46:25 +0000 Subject: [Histonet] New York Qualifications for Histology Supervisor In-Reply-To: <08ec4c97480649daa4e18e192b81eb1a@Exchange.dahlchase.net> References: <30760d2c6a13405089350ea89b6d4a48@MK-EXMB02.mkmg.com> <08ec4c97480649daa4e18e192b81eb1a@Exchange.dahlchase.net> Message-ID: <9a00fb2e8c8e4e1ca9ac8736db7a9398@MK-EXMB02.mkmg.com> Very exciting to hear NSH is working on it with CLIA! I hope the NYS legislature/OOP will take cues from them And I just emailed my rep :) Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager (914) 302-8424 aperl at cmmedical.com -----Original Message----- From: Clare Thornton [mailto:CThornton at dahlchase.com] Sent: Thursday, February 21, 2019 12:39 PM To: 'Kelli Goodkowsky'; Perl , Alison Cc: 'histonet at lists.utsouthwestern.edu' Subject: [EXTERNAL] RE: [Histonet] New York Qualifications for Histology Supervisor NSH is currently working directly with CLIA to addresss issues such as this. Stay tuned! Clare J. Thornton, HTL(ASCP)CM, QIHCCM Lead Immunohistochemistry Tech Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton at dahlchase.com -----Original Message----- From: Kelli Goodkowsky via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 21, 2019 12:11 PM To: Perl , Alison Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] New York Qualifications for Histology Supervisor This is good information, Alison. Thank you for sharing it. Our Histology Program is in CT and I have many students from the southern part of the state, making NY licensing a viable option for them. It would be interesting to note how NY is defining ?judgment? when it comes to the role of histotechnicians/histotechnologists. I sit on the Educator?s Task force for the NSH and will bring this to their attention as well. Kelli Goodkowsky, M.Ed., HT (ASCP) Program Director, Histologic Science President, Faculty Senate Goodwin College (860) 727-6917 kgoodkowsky at goodwin.edu http://www.goodwin.edu On Feb 21, 2019, at 11:51 AM, Perl , Alison via Histonet > wrote: Hi Melissa This is true, and a source of my neverending frustration with NYS Office of Professions. Stephanie Shulman (stephanie.shulman at health.ny.gov) is a good person at NYS to get clarification, but she will reiterate the same. NYS has explained to me that "histotechs don't exercise judgment" and thus are not qualified to take on the role of supervisor, even for a histology-only laboratory. The supervisors here in my lab and myself all have Clinical Lab Technologist licenses, from when they were grandfathered back in 2007. I have techs who went to school specifically for Histology, but can never become supervisors. I don't know what anyone will do for the next generation of supervisors - your situation is a real fear. Anyone in NYS who would like to raise this issue with your representatives, I encourage you to do so!! Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at cmmedical.com -----Original Message----- From: Melissa Owens via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 21, 2019 11:21 AM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] New York Qualifications for Histology Supervisor Hello, I have a question about the requirements to be a supervisor in New York. New York dept. of Ed states qualifications for a Medical Technologist/Laboratory Supervisor and a Cytotechnologist/Cytology Supervisor but no where does it mention qualifications for a Histototech/Histology Supervisor. So therefore, I have heard that in New York, a Histology Supervisor must qualify under the Medical Technologist/Laboratory Supervisor qualifications statues. This seems impossible to me as this would have to be an individual who becomes a Medical Technologist and by random transfers into a histology role then becomes a supervisor. Does anyone out there in New York legally qualify your histology supervisors who have a bachelors degree but were not former Medical Technologists? Thank you for any help as I have intensely scoped the New York department of Education website in my frustrations. By the way, I am asking this because I have a Day Shift 7:30am-4pm Monday-Friday Histology Supervisor position in the New York Metropolitan area with these requirements and I don't know if there is a way around them. Thank you! Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and its attachments, if any, may contain confidential or proprietary information and are intended solely for authorized use by the intended recipient(s) only. Any other use of this email is prohibited. If you have received this email in error, you are hereby notified that any retention, disclosure, copying, forwarding, distribution (in whole or in part and whether electronically, written and/or orally) and/or taking of any action in reliance on this email, its contents and/or any attachments thereto is strictly prohibited. If you received this email in error, please notify the sender by replying to this message and permanently delete this email, and any attachments thereto, from your system immediately. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and its attachments, if any, may contain confidential or proprietary information and are intended solely for authorized use by the intended recipient(s) only. Any other use of this email is prohibited. If you have received this email in error, you are hereby notified that any retention, disclosure, copying, forwarding, distribution (in whole or in part and whether electronically, written and/or orally) and/or taking of any action in reliance on this email, its contents and/or any attachments thereto is strictly prohibited. If you received this email in error, please notify the sender by replying to this message and permanently delete this email, and any attachments thereto, from your system immediately. From marktarango at gmail.com Thu Feb 21 13:05:49 2019 From: marktarango at gmail.com (Mark Tarango) Date: Thu, 21 Feb 2019 11:05:49 -0800 Subject: [Histonet] NTRK positive tissue Message-ID: Would anyone have NTRK1, NTRK2, or NTRK3 positive tissues? We are trying to validate break-apart FISH probes for these gene translocations but don't have any positive tissue. If you have something positive by IHC, I would be very happy to try FISHing it. thank you Mark Tarango CellNetix Pathology Seattle, WA From pruegghm at hotmail.com Thu Feb 21 13:29:02 2019 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Thu, 21 Feb 2019 19:29:02 +0000 Subject: [Histonet] Bone PMMA sections In-Reply-To: <144455abc19d4579b3e31092f8c1fdc4@exmbx01.techosp.it> References: <144455abc19d4579b3e31092f8c1fdc4@exmbx01.techosp.it> Message-ID: Stefano, after cutting the sections, dry them on a heat plate, then they can be stored dried stacked next to each other. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: MANTERO Stefano RIC Sent: Thursday, February 21, 2019 7:08 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Bone PMMA sections Good morning Histonetters! I'm starting to cut out of bone samples included in PMMA. I would like some suggestion how to storage the slides cut. Many thanks in advance Stefano Dott. Stefano Mantero Human Genome Laboratory CNR-IRGB c/o Humanitas Research Hospital Via Rita Levi Montalcini (Ex Via Dainese) 20090 Pieve Emanuele (MI) Ph. +39 02 8224 5164 (desk) Ph. +39 02 8224 5177 (lab) Fax +39 02 8224 5191 Nota di riservatezza. Il presente messaggio, corredato dei relativi allegati, contiene informazioni da considerarsi strettamente riservate, ed ? destinato esclusivamente al destinatario sopra indicato, il quale ? l'unico autorizzato ad usarlo, copiarlo e, sotto la propria responsabilit?, diffonderlo. Chiunque ricevesse questo messaggio per errore o comunque lo leggesse senza esserne legittimato ? avvertito che trattenerlo, copiarlo, divulgarlo, distribuirlo a persone diverse dal destinatario ? severamente proibito, ed ? pregato di rinviarlo immediatamente al mittente distruggendone l'originale. Grazie Confidentiality Notice. This message, together with its annexes, contains information to be deemed strictly confidential and is destined only to the addressee(s) identified above who only may use, copy and, under his/their responsibility, further disseminate it. If anyone received this message by mistake or reads it without entitlement is forewarned that keeping, copying, disseminating or distributing this message to persons other than the addressee(s) is strictly forbidden and is asked to transmit it immediately to the sender and to erase the original message received. Thank You From pruegghm at hotmail.com Thu Feb 21 13:56:21 2019 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Thu, 21 Feb 2019 19:56:21 +0000 Subject: [Histonet] New York Qualifications for Histology Supervisor In-Reply-To: <9a00fb2e8c8e4e1ca9ac8736db7a9398@MK-EXMB02.mkmg.com> References: <30760d2c6a13405089350ea89b6d4a48@MK-EXMB02.mkmg.com> <08ec4c97480649daa4e18e192b81eb1a@Exchange.dahlchase.net>, <9a00fb2e8c8e4e1ca9ac8736db7a9398@MK-EXMB02.mkmg.com> Message-ID: In my opinion this issue is related to the fact that HT's are not required to have BS degrees, like Med Techs and Cytotechs, and that clia/cap does not require ASCP certification to work in a histology lab. We have always been the red headed step child in the lab because of this. This is why NSH has fought so hard to at least require the AS degree with certain science credits. As far as I know, unless it has changed CLIA doesn't even require that those working in histology be ASCP certified. I may be behind on that rule??? I know many very qualified folks who are stars in histology who do not have a BS degree, but I think those times are behind us, as sheep skin seems to count for more than experience in this new world. It is a shame because that sheep skin does not a good Histotech make, on it's own. Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: Perl , Alison Sent: Thursday, February 21, 2019 10:46 AM To: 'Clare Thornton'; 'Kelli Goodkowsky' Cc: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] New York Qualifications for Histology Supervisor Very exciting to hear NSH is working on it with CLIA! I hope the NYS legislature/OOP will take cues from them And I just emailed my rep :) Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager (914) 302-8424 aperl at cmmedical.com -----Original Message----- From: Clare Thornton [mailto:CThornton at dahlchase.com] Sent: Thursday, February 21, 2019 12:39 PM To: 'Kelli Goodkowsky'; Perl , Alison Cc: 'histonet at lists.utsouthwestern.edu' Subject: [EXTERNAL] RE: [Histonet] New York Qualifications for Histology Supervisor NSH is currently working directly with CLIA to addresss issues such as this. Stay tuned! Clare J. Thornton, HTL(ASCP)CM, QIHCCM Lead Immunohistochemistry Tech Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthornton at dahlchase.com -----Original Message----- From: Kelli Goodkowsky via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 21, 2019 12:11 PM To: Perl , Alison Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] New York Qualifications for Histology Supervisor This is good information, Alison. Thank you for sharing it. Our Histology Program is in CT and I have many students from the southern part of the state, making NY licensing a viable option for them. It would be interesting to note how NY is defining ?judgment? when it comes to the role of histotechnicians/histotechnologists. I sit on the Educator?s Task force for the NSH and will bring this to their attention as well. Kelli Goodkowsky, M.Ed., HT (ASCP) Program Director, Histologic Science President, Faculty Senate Goodwin College (860) 727-6917 kgoodkowsky at goodwin.edu http://www.goodwin.edu On Feb 21, 2019, at 11:51 AM, Perl , Alison via Histonet > wrote: Hi Melissa This is true, and a source of my neverending frustration with NYS Office of Professions. Stephanie Shulman (stephanie.shulman at health.ny.gov) is a good person at NYS to get clarification, but she will reiterate the same. NYS has explained to me that "histotechs don't exercise judgment" and thus are not qualified to take on the role of supervisor, even for a histology-only laboratory. The supervisors here in my lab and myself all have Clinical Lab Technologist licenses, from when they were grandfathered back in 2007. I have techs who went to school specifically for Histology, but can never become supervisors. I don't know what anyone will do for the next generation of supervisors - your situation is a real fear. Anyone in NYS who would like to raise this issue with your representatives, I encourage you to do so!! Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager CareMount Medical (914) 302-8424 aperl at cmmedical.com -----Original Message----- From: Melissa Owens via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 21, 2019 11:21 AM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] New York Qualifications for Histology Supervisor Hello, I have a question about the requirements to be a supervisor in New York. New York dept. of Ed states qualifications for a Medical Technologist/Laboratory Supervisor and a Cytotechnologist/Cytology Supervisor but no where does it mention qualifications for a Histototech/Histology Supervisor. So therefore, I have heard that in New York, a Histology Supervisor must qualify under the Medical Technologist/Laboratory Supervisor qualifications statues. This seems impossible to me as this would have to be an individual who becomes a Medical Technologist and by random transfers into a histology role then becomes a supervisor. Does anyone out there in New York legally qualify your histology supervisors who have a bachelors degree but were not former Medical Technologists? Thank you for any help as I have intensely scoped the New York department of Education website in my frustrations. By the way, I am asking this because I have a Day Shift 7:30am-4pm Monday-Friday Histology Supervisor position in the New York Metropolitan area with these requirements and I don't know if there is a way around them. Thank you! Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and its attachments, if any, may contain confidential or proprietary information and are intended solely for authorized use by the intended recipient(s) only. Any other use of this email is prohibited. If you have received this email in error, you are hereby notified that any retention, disclosure, copying, forwarding, distribution (in whole or in part and whether electronically, written and/or orally) and/or taking of any action in reliance on this email, its contents and/or any attachments thereto is strictly prohibited. If you received this email in error, please notify the sender by replying to this message and permanently delete this email, and any attachments thereto, from your system immediately. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and its attachments, if any, may contain confidential or proprietary information and are intended solely for authorized use by the intended recipient(s) only. Any other use of this email is prohibited. If you have received this email in error, you are hereby notified that any retention, disclosure, copying, forwarding, distribution (in whole or in part and whether electronically, written and/or orally) and/or taking of any action in reliance on this email, its contents and/or any attachments thereto is strictly prohibited. If you received this email in error, please notify the sender by replying to this message and permanently delete this email, and any attachments thereto, from your system immediately. From bakevictoria at gmail.com Thu Feb 21 16:48:59 2019 From: bakevictoria at gmail.com (Victoria Baker) Date: Thu, 21 Feb 2019 17:48:59 -0500 Subject: [Histonet] Air pocket in paraffin blocks Message-ID: Hi, When I was trained to do embedding there were many things that the professor stressed to me need to be done in order to have the tissue block acceptable for sectioning. One of these was air bubbles. Recently we had a new tech embed a derm block that had an bubble that was pretty big. The other (experienced) techs didn't think anything of it either and sectioned it. When I got the block for IHC screening I made a QA form stating that the block should have been re-embedded before giving it to be sectioned, or when the first tech sectioned it could have repaired the block or melted it down. This air bubble was big enough to be seen so I don't think it could have been missed - unless the block wasn't checked right after embedding. What has me a little upset is that no one seemed to care about this. I would really appreciate some feedback about this from other people. Thanks in advance. Vikki From bakevictoria at gmail.com Fri Feb 22 03:57:12 2019 From: bakevictoria at gmail.com (Victoria Baker) Date: Fri, 22 Feb 2019 04:57:12 -0500 Subject: [Histonet] Air pocket in paraffin blocks In-Reply-To: References: Message-ID: Yes, it does. The air bubble went directly under the tissue. Vikki On Thu, Feb 21, 2019, 8:43 PM Jennifer Phinney wrote: > Vikki, > Did the air bubble affect the section quality on the slide? If not, would > it have been worth the delay to the case in order to melt and re-embed the > tissue? > > I only re-embed blocks with air bubbles if it will affect the quality of > the slide myself. > > > Jennifer > > -----Original Message----- > From: Victoria Baker via Histonet > Sent: Thursday, February 21, 2019 4:49 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Air pocket in paraffin blocks > > Hi, > > When I was trained to do embedding there were many things that the > professor stressed to me need to be done in order to have the tissue block > acceptable for sectioning. One of these was air bubbles. > > Recently we had a new tech embed a derm block that had an bubble that was > pretty big. The other (experienced) techs didn't think anything of it > either and sectioned it. When I got the block for IHC screening I made a > QA form stating that the block should have been re-embedded before giving > it to be sectioned, or when the first tech sectioned it could have repaired > the block or melted it down. This air bubble was big enough to be seen so > I don't think it could have been missed - unless the block wasn't checked > right after embedding. > > What has me a little upset is that no one seemed to care about this. > > I would really appreciate some feedback about this from other people. > > Thanks in advance. > > Vikki > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histo at pathlab.us Fri Feb 22 09:34:36 2019 From: histo at pathlab.us (Histology) Date: Fri, 22 Feb 2019 15:34:36 +0000 Subject: [Histonet] Not DAB IHC waste Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD820714A72E@vdc01.domain.local> Hi all, Does anyone use only red or magenta detection for IHCs instead of DAB? We do not use DAB and are trying to figure out if our waste is hazardous or not. Any insight would be helpful. Thanks, Mehndi Helgren From shive003 at umn.edu Fri Feb 22 10:11:46 2019 From: shive003 at umn.edu (Jan Shivers) Date: Fri, 22 Feb 2019 10:11:46 -0600 Subject: [Histonet] Not DAB IHC waste In-Reply-To: <09CFA3F99D5B2B42B88CDFB2FC4CFD820714A72E@vdc01.domain.local> References: <09CFA3F99D5B2B42B88CDFB2FC4CFD820714A72E@vdc01.domain.local> Message-ID: Hello, It depends upon which 'red' chromogen you are using. There are several with different chemical compositions, so you will need to look at the Safety Data Sheets which pertain to the chemical you are using. In my lab we use 3-amino-9-ethylcarbazole (AEC) which is considered hazardous. It causes eye and skin irritation, may cause serious eye damage, and may cause cancer. We ship our waste off for proper disposal; it does not go down the water drain. Best regards, Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003 at umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Fri, Feb 22, 2019 at 9:34 AM Histology via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all, > > Does anyone use only red or magenta detection for IHCs instead of DAB? We > do not use DAB and are trying to figure out if our waste is hazardous or > not. Any insight would be helpful. > > Thanks, > > Mehndi Helgren > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From terrie.dalton at icloud.com Fri Feb 22 10:31:50 2019 From: terrie.dalton at icloud.com (Theresa Dalton) Date: Fri, 22 Feb 2019 11:31:50 -0500 Subject: [Histonet] Air pocket in paraffin blocks In-Reply-To: References: Message-ID: Oh no! Absolutely re-embed. Sent from my iPhone > On Feb 22, 2019, at 4:57 AM, Victoria Baker via Histonet wrote: > > Yes, it does. The air bubble went directly under the tissue. > > Vikki > > On Thu, Feb 21, 2019, 8:43 PM Jennifer Phinney > wrote: > >> Vikki, >> Did the air bubble affect the section quality on the slide? If not, would >> it have been worth the delay to the case in order to melt and re-embed the >> tissue? >> >> I only re-embed blocks with air bubbles if it will affect the quality of >> the slide myself. >> >> >> Jennifer >> >> -----Original Message----- >> From: Victoria Baker via Histonet >> Sent: Thursday, February 21, 2019 4:49 PM >> To: histonet at lists.utsouthwestern.edu >> Subject: [Histonet] Air pocket in paraffin blocks >> >> Hi, >> >> When I was trained to do embedding there were many things that the >> professor stressed to me need to be done in order to have the tissue block >> acceptable for sectioning. One of these was air bubbles. >> >> Recently we had a new tech embed a derm block that had an bubble that was >> pretty big. The other (experienced) techs didn't think anything of it >> either and sectioned it. When I got the block for IHC screening I made a >> QA form stating that the block should have been re-embedded before giving >> it to be sectioned, or when the first tech sectioned it could have repaired >> the block or melted it down. This air bubble was big enough to be seen so >> I don't think it could have been missed - unless the block wasn't checked >> right after embedding. >> >> What has me a little upset is that no one seemed to care about this. >> >> I would really appreciate some feedback about this from other people. >> >> Thanks in advance. >> >> Vikki >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jose.R.deGuzman at gunet.georgetown.edu Fri Feb 22 12:32:41 2019 From: Jose.R.deGuzman at gunet.georgetown.edu (deGuzman, Jose R) Date: Fri, 22 Feb 2019 18:32:41 +0000 Subject: [Histonet] Air pocket in paraffin blocks In-Reply-To: References: Message-ID: Hello Vikki, What you are experiencing is the lack of quality feedback. The "experienced" techs may have never been told that their quality needs to improve due to complacency or management is afraid they might leave. This gets worse if they have gotten away with producing poor quality for a long time and nobody has provided much needed feedback to them. You may have an uphill battle ahead of you but very much worth taking. Jose -----Original Message----- From: Victoria Baker Sent: Thursday, February 21, 2019 5:49 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Air pocket in paraffin blocks Hi, When I was trained to do embedding there were many things that the professor stressed to me need to be done in order to have the tissue block acceptable for sectioning. One of these was air bubbles. Recently we had a new tech embed a derm block that had an bubble that was pretty big. The other (experienced) techs didn't think anything of it either and sectioned it. When I got the block for IHC screening I made a QA form stating that the block should have been re-embedded before giving it to be sectioned, or when the first tech sectioned it could have repaired the block or melted it down. This air bubble was big enough to be seen so I don't think it could have been missed - unless the block wasn't checked right after embedding. What has me a little upset is that no one seemed to care about this. I would really appreciate some feedback about this from other people. Thanks in advance. Vikki From patpxs at gmail.com Fri Feb 22 14:00:43 2019 From: patpxs at gmail.com (P Sicurello) Date: Fri, 22 Feb 2019 12:00:43 -0800 Subject: [Histonet] [External] Re: New York Qualifications for Histology Supervisor In-Reply-To: <1550842232189.88291@uhsinc.com> References: <30760d2c6a13405089350ea89b6d4a48@MK-EXMB02.mkmg.com> <1550842232189.88291@uhsinc.com> Message-ID: I need to clarify my comment about California. What Histotechs (certified or not, but not licensed by CA) cannot do is supervise or manage those who have licenses through the state (Cyotechs, CLS', Phlebotomists, etc.). Unlicensed personnel are not allowed to supervise/manage licensed personnel. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Fri, Feb 22, 2019 at 5:30 AM Fortin, Joyce wrote: > omg!! > > Joyce Fortin > Histology Supervisor > Palmdale Regional Medical Center > 38600 Medical Center Drive > Palmdale, California 93551 > Phone 661-382-5723 > Fax 661-382-5747 > email: joyce.fortin at uhsinc.com > > > ________________________________________ > From: Patpxs via Histonet > Sent: Thursday, February 21, 2019 9:18 AM > To: Colleen Forster > Cc: histonet at lists.utsouthwestern.edu > Subject: [External] Re: [Histonet] New York Qualifications for Histology > Supervisor > > WARNING: This email is from an external source. > DO NOT CLICK links or attachments unless you recognize the sender and know > the content is safe. > REPORT suspicious emails to ReportSpam at uhsinc.com ASAP. > > This is an issue that continues to pop up. It all starts with the CLIA > regulations that do not recognize histology professionals as equal to other > laboratory professionals. Until the feds change CLIA we will keep having > this problem. > > I do believe that NSH is petitioning on our behalf to change the CLIA > regulations. > > In California the state does not recognize Histotechs, certified or not, > so we can?t get licensed and we can?t become lab managers as a result. All > thanks to CLIA. > > That?s my rant for the day. > > Paula > > Sent from my iPhone > > > On Feb 21, 2019, at 8:58 AM, Colleen Forster via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > > Wow...this is just crazy~ The regulations leave histology in a real tough > > spot... > > > > Feeling for all of you and yes, for the future histology techs coming up~ > > > > Colleen Forster > > U of MN > > > > On Thu, Feb 21, 2019 at 10:52 AM Perl , Alison via Histonet < > > histonet at lists.utsouthwestern.edu> wrote: > > > >> Hi Melissa > >> This is true, and a source of my neverending frustration with NYS Office > >> of Professions. Stephanie Shulman (stephanie.shulman at health.ny.gov) is > a > >> good person at NYS to get clarification, but she will reiterate the > same. > >> > >> NYS has explained to me that "histotechs don't exercise judgment" and > thus > >> are not qualified to take on the role of supervisor, even for a > >> histology-only laboratory. The supervisors here in my lab and myself all > >> have Clinical Lab Technologist licenses, from when they were > grandfathered > >> back in 2007. I have techs who went to school specifically for > Histology, > >> but can never become supervisors. I don't know what anyone will do for > the > >> next generation of supervisors - your situation is a real fear. > >> > >> Anyone in NYS who would like to raise this issue with your > >> representatives, I encourage you to do so!! > >> > >> Alison Perl, HTL(ASCP)CM > >> Anatomic Pathology Manager > >> CareMount Medical > >> (914) 302-8424 > >> aperl at cmmedical.com > >> > >> > >> -----Original Message----- > >> From: Melissa Owens via Histonet [mailto: > histonet at lists.utsouthwestern.edu] > >> > >> Sent: Thursday, February 21, 2019 11:21 AM > >> To: histonet at lists.utsouthwestern.edu > >> Subject: [EXTERNAL] [Histonet] New York Qualifications for Histology > >> Supervisor > >> > >> Hello, > >> > >> I have a question about the requirements to be a supervisor in New York. > >> New York dept. of Ed states qualifications for a Medical > >> Technologist/Laboratory Supervisor and a Cytotechnologist/Cytology > >> Supervisor but no where does it mention qualifications for a > >> Histototech/Histology Supervisor. So therefore, I have heard that in New > >> York, a Histology Supervisor must qualify under the Medical > >> Technologist/Laboratory Supervisor qualifications statues. This seems > >> impossible to me as this would have to be an individual who becomes a > >> Medical Technologist and by random transfers into a histology role then > >> becomes a supervisor. Does anyone out there in New York legally qualify > >> your histology supervisors who have a bachelors degree but were not > former > >> Medical Technologists? Thank you for any help as I have intensely scoped > >> the New York department of Education website in my frustrations. > >> > >> By the way, I am asking this because I have a Day Shift 7:30am-4pm > >> Monday-Friday Histology Supervisor position in the New York Metropolitan > >> area with these requirements and I don't know if there is a way around > >> them. Thank you! > >> > >> Melissa Owens, CHP (ASA) > >> President, Laboratory Staffing > >> Allied Search Partners > >> > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> This email and its attachments, if any, may contain confidential or > >> proprietary information and are intended solely for authorized use by > the > >> intended recipient(s) only. Any other use of this email is prohibited. > If > >> you have received this email in error, you are hereby notified that any > >> retention, disclosure, copying, forwarding, distribution (in whole or in > >> part and whether electronically, written and/or orally) and/or taking of > >> any action in reliance on this email, its contents and/or any > attachments > >> thereto is strictly prohibited. If you received this email in error, > please > >> notify the sender by replying to this message and permanently delete > this > >> email, and any attachments thereto, from your system immediately. > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > > -- > > Colleen Forster HT(ASCP)QIHC > > BLS Histology and IHC Laboratory > > B173 PWB 612-626-1930 > > > > *If submitting histology request please also forward to Lori Holm at > > holml at umn.edu * > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > UHS of Delaware, Inc. Confidentiality Notice: This e-mail message, > including any attachments, is for the sole use of the intended recipient(s) > and may contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution of this information is prohibited, > and may be punishable by law. If this was sent to you in error, please > notify the sender by reply e-mail and destroy all copies of the original > message. > From jmyers1 at aol.com Fri Feb 22 15:33:09 2019 From: jmyers1 at aol.com (Joe Myers) Date: Fri, 22 Feb 2019 16:33:09 -0500 Subject: [Histonet] Not DAB IHC waste Message-ID: <4E9AAFBF-72AC-4A23-8C60-A515CF13A09C@aol.com> Mehndi: I?ve used products like the ones referring to in many labs, over many years, and although it?s difficult to find explicit references stating that Fast Red (and Fast Blue)-type chromogens are truly hazardous/carcinogenic, like DAB, these are all very complex chemicals that we, as responsible scientists and citizens, should probably refrain from putting into the public sewer system/water supply. I would, therefore, recommend that your lab treat such waste-reagents as hazardous and dispose of them accordingly. Respectfully, Joe Myers, M.S., CT(ASCP)QIHC Message: 6 Date: Fri, 22 Feb 2019 15:34:36 +0000 From: Histology To: "histonet at lists.utsouthwestern.edu" Hi all, Does anyone use only red or magenta detection for IHCs instead of DAB? We do not use DAB and are trying to figure out if our waste is hazardous or not. Any insight would be helpful. Thanks, Mehndi Helgren From Monica.Lockhart at luhs.org Mon Feb 25 11:33:33 2019 From: Monica.Lockhart at luhs.org (MONICA D. LOCKHART) Date: Mon, 25 Feb 2019 17:33:33 +0000 Subject: [Histonet] Muscle Enzyme Histochemistry Message-ID: <5ab0d33096f44da1bd81ad68d5be33c8@luhs.org> Hello Histology World!!! Can muscle placed in Gluteraldehyde be processed for structure? In this situation, all tissue was placed in Glut and we need to perform Enzyme Histochemistry but we have no fresh tissue. Thanks for your help. Monica D. Lockhart, BBA, HT (ASCP) PBT Supervisor Clinical Labs Histology Loyola University Medical Center 2160 S. First Ave, Bldg 110 Rm 2290 Maywood, IL 60153 (o) 708.327.2608 (c) 708.692.8361 monica.lockhart at luhs.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From srishan at mail.holyname.org Mon Feb 25 12:56:54 2019 From: srishan at mail.holyname.org (Nirmala Srishan) Date: Mon, 25 Feb 2019 13:56:54 -0500 Subject: [Histonet] TISSUE PUNCH FOR MAKING CONTROL BLOCKS Message-ID: Hi All, Could some one give a good tissue punch product for making control blocks for IHC? Thanks Nirmala Srishan Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From khong at waxitinc.com Mon Feb 25 12:58:40 2019 From: khong at waxitinc.com (khong at waxitinc.com) Date: Mon, 25 Feb 2019 10:58:40 -0800 Subject: [Histonet] Leica bond Max In-Reply-To: References: Message-ID: <002a01d4cd3c$1fd93f10$5f8bbd30$@com> Hi all, Is there anyone interesting to the Leica bond max IHC stainer? Thanks, Kai Hong Research histotechnologist khong at waxitinc.com 202 - 2386 East Mall Vancouver, B.C. V6T 1Z3 Canada Office: 604-822-1595 Lab: 604-822-2589 Fax: 604-822-0660 www.waxitinc.com -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Monday, February 25, 2019 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 183, Issue 20 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Muscle Enzyme Histochemistry (MONICA D. LOCKHART) ---------------------------------------------------------------------- Message: 1 Date: Mon, 25 Feb 2019 17:33:33 +0000 From: "MONICA D. LOCKHART" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Muscle Enzyme Histochemistry Message-ID: <5ab0d33096f44da1bd81ad68d5be33c8 at luhs.org> Content-Type: text/plain; charset="us-ascii" Hello Histology World!!! Can muscle placed in Gluteraldehyde be processed for structure? In this situation, all tissue was placed in Glut and we need to perform Enzyme Histochemistry but we have no fresh tissue. Thanks for your help. Monica D. Lockhart, BBA, HT (ASCP) PBT Supervisor Clinical Labs Histology Loyola University Medical Center 2160 S. First Ave, Bldg 110 Rm 2290 Maywood, IL 60153 (o) 708.327.2608 (c) 708.692.8361 monica.lockhart at luhs.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 183, Issue 20 ***************************************** From llewllew at shaw.ca Mon Feb 25 14:37:16 2019 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Mon, 25 Feb 2019 12:37:16 -0800 Subject: [Histonet] Muscle Enzyme Histochemistry In-Reply-To: <5ab0d33096f44da1bd81ad68d5be33c8@luhs.org> References: <5ab0d33096f44da1bd81ad68d5be33c8@luhs.org> Message-ID: <46b280a9-0e3d-9faa-c223-b66282b2fa7b@shaw.ca> Hi, I presume you are talking about light microscopy. Glutaraldehyde is often used for electron microscopy. If that is the case, ignore this. Glutataraldehyde fixes similarly to formalin, so the morphology should not be much different. However, it leaves the tissue with free aldehyde groups and these can react with Schiff's reagent or silver reductions. That means if doing a PAS or methenamine silver you should first (before oxidation) do an aldehyde block. I recommend aniline-acetic acid for 30 minutes, although others work as well. If you don't you will have a dark background. It is unlikely that enzyme histochemistry will be successful, but you never know. Esterase may still be demonstrable, possibly, but the ATPase iso-enzymes are unlikely to survive. Go to http://stainsfile.info and read the pages on glutaraldehyde for more information about it. Bryan Llewellyn MONICA D. LOCKHART via Histonet wrote: > Hello Histology World!!! > > Can muscle placed in Gluteraldehyde be processed for structure? In this situation, all tissue was placed in Glut and we need to perform Enzyme Histochemistry but we have no fresh tissue. > > Thanks for your help. > > Monica D. Lockhart, BBA, HT (ASCP) PBT > Supervisor Clinical Labs Histology > Loyola University Medical Center > 2160 S. First Ave, Bldg 110 Rm 2290 > Maywood, IL 60153 > (o) 708.327.2608 > (c) 708.692.8361 > monica.lockhart at luhs.org > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 at earthlink.net Tue Feb 26 10:33:20 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 26 Feb 2019 11:33:20 -0500 Subject: [Histonet] RELIA Hot Histology Job Alert!! Grossing Histotechnologist - Days Growing Lab in Florida - A RELIA Exclusive!!! Message-ID: <000001d4cdf0$fcbce530$f636af90$@earthlink.net> Hi Histonetters!! I hope everyone is having a great day! I have an exclusive opportunity with one of my best clients who also happens to be one of the BEST places to work in Florida. Here?s the info: RELIA has been engaged EXCLUSIVELY by a growing Private Dermatopathology lab in Southwest Florida that is in need of a Florida licensed HTL who is CLIA qualified to gross. This is a Full time DAY shift position and my client offers an excellent pay rate, amazing benefits and one of the BEST places to work in FLORIDA!!! For more info: CALL/TEXT ME: 407-353-5070 EMAIL ME: relia1 at earthlink.net Thanks-Pam #jobs4myhistopeeps Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Timothy.Morken at ucsf.edu Tue Feb 26 10:49:22 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 26 Feb 2019 16:49:22 +0000 Subject: [Histonet] Waffle-pattern bench paper? Message-ID: Hi all, I used to get bench protector paper (paper and plastic backing) from Cardinal that had a waffle pattern. We really like it. Now they substituted it with a really flimsy paper surface that constantly bunches up whereas the other type did not. Does anyone know where to get the other type of bench paper? Any help is appreciated! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From Timothy.Morken at ucsf.edu Tue Feb 26 13:20:15 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 26 Feb 2019 19:20:15 +0000 Subject: [Histonet] Temp for muscle FS and histochem, possibly EM. Message-ID: If you do temp work and are looking around for the next position, we are looking for at least a 3-month temp specifically for muscle histochem - frozen sectioning and histochemistry. Will also do kidney FS and IF staining on the Bond. Or, in addition, if you know how to do EM sectioning that would be useful. If interested let me know. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From Erin.Martin at ucsf.edu Wed Feb 27 08:19:16 2019 From: Erin.Martin at ucsf.edu (Martin, Erin) Date: Wed, 27 Feb 2019 14:19:16 +0000 Subject: [Histonet] Stain consulting services Message-ID: Good morning all! We are having a problem with our H&E. My chief pathologist believes that we have day to day variations in intensity as well as variations from slide to slide (even within a rack), and that the hematoxylin gets darker as the week goes on. We use 2 automated Sakura Prisma strainers and now use all pre-made, purchased solutions (to rule out human error in prep). We run A LOT of controls, 4 (different blocks) on each stainer at the beginning of the day before patient cases, another set after all patient cases, and then another set after we rotate the reagents at the end of the day, for a total of 24 per day. Unfortunately the staining on all the controls looks the same and my chief pathologist says that control slides are not useful in the illustration of the problem. So I have two questions from him: 1. Have those using Richard Allan Hematoxylin 7212 noticed this problem? He believes that there must have been a change in formulation or quality 2. Does anyone know of a consulting service that will come in and work on this problem? I have made all the changes and QC checks that I can think of. The problem is obviously outside of my ability to solve. Thank you in advance for any advice or consulting recommendations! Have a lovely Wednesday! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero Street, RM 230 San Francisco, CA 94115 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. From relia1 at earthlink.net Wed Feb 27 09:24:31 2019 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 27 Feb 2019 10:24:31 -0500 Subject: [Histonet] 14 years!!!! Can You Believe It? Thank you!!!!! Message-ID: <000001d4ceb0$89b89b90$9d29d2b0$@earthlink.net> Hi Histonetters, How are you doing today? This is the start to an exciting week for me! This week we are celebrating RELIA?s 14th year in business. Thank You!! For the past 14 years we have worked exclusively in the nationwide permanent placement of histology professionals. I have felt so privileged to have helped so many people with their careers and look forward to helping many more! We strive to bring you the best histology opportunities out there. I know your inbox is precious real estate and I appreciate you taking the time to read my emails. I also have some exciting job opportunities to pass along. Please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral reward! I LOVE TO GIVE REFERRAL REWARDS!! SOME of these are RELIA exclusives MOST of these offer Sign- on Bonuses and/or Relocation Assistance ALL of these Companies offer excellent compensation, benefits and great environments. AND THEY ALL ARE READY TO HIRE!! If You Or Anyone You Know Might Be Interested In Any Of These Positions Please Contact Me. You can reach me by email at relia1 at earthlink.net Toll free at 866-607-3542 or on my cell at 407-353-5070. Here is my Spotlight Opportunity: I have been engaged by a growing Private Dermatopathology lab in Southwest Florida that is in need of a Florida licensed HTL who is CLIA qualified to gross. This is a Full time DAY shift position and my client offers an excellent pay rate, amazing benefits and one of the BEST places to work in FLORIDA!!! TEXT ME/ EMAIL ME/ CALL ME For more info! We Have Exciting Opportunities In: ? Florida ? Ohio ? Maryland ? Massachusetts ? Michigan ? Wisconsin ? Alabama ? California ? New York Shoot me an email at relia1 at earthlink.net for details on any of these opportunities! Have a great day. I look forward to hearing back from you. Thanks-Pam #jobs4myhistopeeps Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From acoscetti at gmail.com Wed Feb 27 10:24:02 2019 From: acoscetti at gmail.com (Amanda Coscetti) Date: Wed, 27 Feb 2019 11:24:02 -0500 Subject: [Histonet] Cytology processing Message-ID: Hi everyone! Wondering if there are any other companies besides Hologic/Cytyc for processing non-gyn cytologies. We have an old Thinprep2000 and are looking to upgrade soon. Thanks! Amanda Histotech McLeod Health From criley at dpspa.com Wed Feb 27 13:47:07 2019 From: criley at dpspa.com (Charles Riley) Date: Wed, 27 Feb 2019 14:47:07 -0500 Subject: [Histonet] H&E Staining question Message-ID: Has anyone tried using Hologic's Thin Prep solutions (Nuclear stain, rinse, and bluing) to do their H&E stains? I am trying to validate a new stainer and in the process the pathologists want to tweak our protocol to get a better stain and thought maybe we could try this to see about saving money in the process -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From jaylundgren at gmail.com Wed Feb 27 14:39:51 2019 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 27 Feb 2019 14:39:51 -0600 Subject: [Histonet] H&E Staining question In-Reply-To: References: Message-ID: Obviously, you're going to get a much different result using a polychromatic stain, than by doing a standard H&E. But if the pathologists like it... I'm not even sure if they could call that stain an H&E in their dictation. Seems like that would open them up to liability if, God forbid, they ever missed something. I can just hear the medical malpractice attorney now, "You did what? And you decided on this stain based on what? Here's what our expert witness found when he stained sections of this block with H&E, and here's what your protocol shows." Worse case scenario, I know, but there's a reason old school pathologists are so hidebound. On Wed, Feb 27, 2019 at 2:03 PM Charles Riley via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Has anyone tried using Hologic's Thin Prep solutions (Nuclear stain, rinse, > and bluing) to do their H&E stains? > > I am trying to validate a new stainer and in the process the pathologists > want to tweak our protocol to get a better stain and thought maybe we could > try this to see about saving money in the process > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From CDavis at che-east.org Thu Feb 28 09:23:16 2019 From: CDavis at che-east.org (Cassie P. Davis) Date: Thu, 28 Feb 2019 15:23:16 +0000 Subject: [Histonet] FYI- Roche Ventana users Message-ID: Hi Histoland, I am biting my tongue HARD and just letting you know so it doesn't happend to you. I just got off the phone with Roche here is the heads-up. If one of their anitbody dispensers fails DO NOT put the antibody in one of their prep kits, as soon as you do they consider it off label use. Call customer service immediately and have them overnight a replacement! Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From sandra.cheasty at wisc.edu Thu Feb 28 12:56:43 2019 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Thu, 28 Feb 2019 18:56:43 +0000 Subject: [Histonet] IHC Control Slide Longevity Message-ID: Hello all, Do any of you have to cut any of you antibody controls "fresh"? We currently cut the MUM-1 fresh, and the Synaptophysin. (We seldom do a Syanpt., and have found the fresh cut patient tissue has been positive while the not-so-fresh positive control slide is negative. Any comments are - as always, are appreciated. Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From tbraud at holyredeemer.com Thu Feb 28 13:21:53 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 28 Feb 2019 19:21:53 +0000 Subject: [Histonet] Another Dispenser Failure Message-ID: <48E053DDF6CE074DB6A7414BA05403F8013BEE27A8@HRHEX02-HOS.holyredeemer.local> Another one!!! Our Her2 antibody dispenser failed, LOT #E22628 This one "supposedly" FDA approved. Roche, why do you continue to lie to consumers of your product? You claim you've "fixed" the problem but your Ventana dispensers DON'T WORK! This is patient care! Why don't you care about the customers and patients you are supposed to be serving???? Shame on you. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, February 28, 2019 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 183, Issue 23 Today's Topics: 1. H&E Staining question (Charles Riley) 2. Re: H&E Staining question (Jay Lundgren) 3. FYI- Roche Ventana users (Cassie P. Davis) Message: 3 Date: Thu, 28 Feb 2019 15:23:16 +0000 From: "Cassie P. Davis" To: histonet Subject: [Histonet] FYI- Roche Ventana users Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histoland, I am biting my tongue HARD and just letting you know so it doesn't happend to you. I just got off the phone with Roche here is the heads-up. If one of their anitbody dispensers fails DO NOT put the antibody in one of their prep kits, as soon as you do they consider it off label use. Call customer service immediately and have them overnight a replacement! Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org From shive003 at umn.edu Thu Feb 28 13:29:04 2019 From: shive003 at umn.edu (Jan Shivers) Date: Thu, 28 Feb 2019 13:29:04 -0600 Subject: [Histonet] IHC Control Slide Longevity In-Reply-To: References: Message-ID: Hi Sandy, We cut CD31, CD45, CD45RA, CD117, CD204, and MUM1 controls fresh (same morning as staining; case material as well). We've never had any problems with Synaptophysin; it's always worked very well on unstained controls that are up to a year old (stored in the fridge). Best regards, Jan Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003 at umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Thu, Feb 28, 2019 at 12:57 PM Sandra Cheasty via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello all, > Do any of you have to cut any of you antibody controls > "fresh"? We currently cut the MUM-1 fresh, and the Synaptophysin. (We > seldom do a Syanpt., and have found the fresh cut patient tissue has been > positive while the not-so-fresh positive control slide is negative. > Any comments are - as always, are appreciated. > Cheers, > Sandy > > Sandra J. Cheasty, HT (ASCP) > Histology & Necropsy Supervisor > UW-Madison, School of Veterinary Medicine > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Nancy_Schmitt at pa-ucl.com Thu Feb 28 14:28:42 2019 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Thu, 28 Feb 2019 20:28:42 +0000 Subject: [Histonet] Surepath stains Message-ID: Hello- This is regarding Surepath pap stain processing on the BD Totalys. We switched to the new Totalys in late November and are having a lot of staining issues. We have had many visits with field service. Is anyone using this and having issues with inconsistent staining? Thank you for your consideration, Nancy United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From marktarango at gmail.com Thu Feb 28 14:30:03 2019 From: marktarango at gmail.com (Mark Tarango) Date: Thu, 28 Feb 2019 12:30:03 -0800 Subject: [Histonet] Another Dispenser Failure In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8013BEE27A8@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8013BEE27A8@HRHEX02-HOS.holyredeemer.local> Message-ID: I hope everyone is using on-slide controls :-) On Thu, Feb 28, 2019 at 11:52 AM Terri Braud via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Another one!!! > Our Her2 antibody dispenser failed, LOT #E22628 > This one "supposedly" FDA approved. > Roche, why do you continue to lie to consumers of your product? You claim > you've "fixed" the problem but your Ventana dispensers DON'T WORK! > This is patient care! Why don't you care about the customers and patients > you are supposed to be serving???? > Shame on you. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > -----Original Message----- > From: histonet-request at lists.utsouthwestern.edu [mailto: > histonet-request at lists.utsouthwestern.edu] > Sent: Thursday, February 28, 2019 1:00 PM > To: histonet at lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 183, Issue 23 > > > Today's Topics: > 1. H&E Staining question (Charles Riley) > 2. Re: H&E Staining question (Jay Lundgren) > 3. FYI- Roche Ventana users (Cassie P. Davis) > Message: 3 > Date: Thu, 28 Feb 2019 15:23:16 +0000 > From: "Cassie P. Davis" > To: histonet > Subject: [Histonet] FYI- Roche Ventana users > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histoland, > I am biting my tongue HARD and just letting you know so it doesn't > happend to you. I just got off the phone with Roche here is the heads-up. > If one of their anitbody dispensers fails DO NOT put the antibody in one > of their prep kits, as soon as you do they consider it off label use. > Call customer service immediately and have them overnight a replacement! > Cassandra Davis > Histology Technician > AP Laboratory > 302-575-8095 > Email: CDavis at che-east.org > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akbitting at geisinger.edu Thu Feb 28 15:17:58 2019 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Thu, 28 Feb 2019 21:17:58 +0000 Subject: [Histonet] [External] Re: Another Dispenser Failure In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F8013BEE27A8@HRHEX02-HOS.holyredeemer.local>, Message-ID: Is there any other way! :-O Sent from my iPhone > On Feb 28, 2019, at 3:30 PM, Mark Tarango via Histonet wrote: > > I hope everyone is using on-slide controls :-) > > On Thu, Feb 28, 2019 at 11:52 AM Terri Braud via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Another one!!! >> Our Her2 antibody dispenser failed, LOT #E22628 >> This one "supposedly" FDA approved. >> Roche, why do you continue to lie to consumers of your product? You claim >> you've "fixed" the problem but your Ventana dispensers DON'T WORK! >> This is patient care! Why don't you care about the customers and patients >> you are supposed to be serving???? >> Shame on you. >> >> Terri L. Braud, HT(ASCP) >> Anatomic Pathology Supervisor >> Laboratory >> Holy Redeemer Hospital >> 1648 Huntingdon Pike >> Meadowbrook, PA 19046 >> ph: 215-938-3689 >> fax: 215-938-3874 >> Care, Comfort, and Heal >> >> -----Original Message----- >> From: histonet-request at lists.utsouthwestern.edu [mailto: >> histonet-request at lists.utsouthwestern.edu] >> Sent: Thursday, February 28, 2019 1:00 PM >> To: histonet at lists.utsouthwestern.edu >> Subject: Histonet Digest, Vol 183, Issue 23 >> >> >> Today's Topics: >> 1. H&E Staining question (Charles Riley) >> 2. Re: H&E Staining question (Jay Lundgren) >> 3. FYI- Roche Ventana users (Cassie P. Davis) >> Message: 3 >> Date: Thu, 28 Feb 2019 15:23:16 +0000 >> From: "Cassie P. Davis" >> To: histonet >> Subject: [Histonet] FYI- Roche Ventana users >> Message-ID: >> Content-Type: text/plain; charset="iso-8859-1" >> >> Hi Histoland, >> I am biting my tongue HARD and just letting you know so it doesn't >> happend to you. I just got off the phone with Roche here is the heads-up. >> If one of their anitbody dispensers fails DO NOT put the antibody in one >> of their prep kits, as soon as you do they consider it off label use. >> Call customer service immediately and have them overnight a replacement! >> Cassandra Davis >> Histology Technician >> AP Laboratory >> 302-575-8095 >> Email: CDavis at che-east.org >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cakbitting%40geisinger.edu%7C54f99c969c034efc787c08d69dbb9222%7C37d46c567c664402a16055c2313b910d%7C0%7C0%7C636869826267838962&sdata=Fa%2BIazZR5ONBVw8DF35Yib5Ev1RTbpySxNuVjo0NobY%3D&reserved=0 >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cakbitting%40geisinger.edu%7C54f99c969c034efc787c08d69dbb9222%7C37d46c567c664402a16055c2313b910d%7C0%7C0%7C636869826267848970&sdata=%2Fdu5DB7SPsE5RRHX9hpCNyGopDRovFnxf9NwQZmjsu4%3D&reserved=0 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From c.tague at Pathologyarts.com Thu Feb 28 15:30:20 2019 From: c.tague at Pathologyarts.com (Curt) Date: Thu, 28 Feb 2019 21:30:20 +0000 Subject: [Histonet] Another Dispenser Failure In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F8013BEE27A8@HRHEX02-HOS.holyredeemer.local>, Message-ID: <9C8F910F72893643B3C3793C3D67132B94D189B5@PATHOLOGYSERVER.pathologyarts.local> It's the money they care about... Sent from my Verizon, Samsung Galaxy smartphone -------- Original message -------- From: Mark Tarango via Histonet Date: 2/28/19 12:46 PM (GMT-08:00) To: Terri Braud Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Another Dispenser Failure I hope everyone is using on-slide controls :-) On Thu, Feb 28, 2019 at 11:52 AM Terri Braud via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Another one!!! > Our Her2 antibody dispenser failed, LOT #E22628 > This one "supposedly" FDA approved. > Roche, why do you continue to lie to consumers of your product? You claim > you've "fixed" the problem but your Ventana dispensers DON'T WORK! > This is patient care! Why don't you care about the customers and patients > you are supposed to be serving???? > Shame on you. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > -----Original Message----- > From: histonet-request at lists.utsouthwestern.edu [mailto: > histonet-request at lists.utsouthwestern.edu] > Sent: Thursday, February 28, 2019 1:00 PM > To: histonet at lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 183, Issue 23 > > > Today's Topics: > 1. H&E Staining question (Charles Riley) > 2. Re: H&E Staining question (Jay Lundgren) > 3. FYI- Roche Ventana users (Cassie P. Davis) > Message: 3 > Date: Thu, 28 Feb 2019 15:23:16 +0000 > From: "Cassie P. Davis" > To: histonet > Subject: [Histonet] FYI- Roche Ventana users > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histoland, > I am biting my tongue HARD and just letting you know so it doesn't > happend to you. I just got off the phone with Roche here is the heads-up. > If one of their anitbody dispensers fails DO NOT put the antibody in one > of their prep kits, as soon as you do they consider it off label use. > Call customer service immediately and have them overnight a replacement! > Cassandra Davis > Histology Technician > AP Laboratory > 302-575-8095 > Email: CDavis at che-east.org > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. From Richard.Cartun at hhchealth.org Thu Feb 28 16:47:46 2019 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Thu, 28 Feb 2019 22:47:46 +0000 Subject: [Histonet] Ventana p16 antibody Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EAC10FF98@HHCEXCHMB03.hhcsystem.org> We use the Roche/Ventana p16 antibody (clone E6H4(tm)) on the Leica Bond Max. We are having a difficult time trying to order the antibody. Can someone verify the catalog number (705-4713) for me? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments.