From melissa at alliedsearchpartners.com Tue Dec 3 09:50:59 2019 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Tue, 3 Dec 2019 15:50:59 +0000 Subject: [Histonet] Histology/Cytology Job Openings Update & Suggestion for other Firms on Here Message-ID: Hello Histonet, Last time I posted my jobs update, there were just under a dozen other firms almost "immediately" following my post, email about their job openings too, and it seemed as though that happened just because I posted mine. Could we kindly have a professional interaction on here among fellow recruiters and not post our jobs just because another firm posted their jobs? Just a suggestion. I find it a privilege to be on the histonet in the first place. Always a pleasure to hear from everyone. I have the following positions available for permanent positions. In addition, my firm, has recently joined as an affiliate with MRI Network, global staffing and we will also be offering travel tech work. Please let me know if you are looking for travel assignments. Thank you, . Permanent- 1. Histology Supervisor-Santa Ana, CA area 2. Histotech-Birmingham, AL area 3. IHC Supervisor-Bronx, NY area 4. Cytotechnologist-Johnson City, TN area Melissa Owens Allied Search Partners AN MRINETWORK MEMBER Direct (Call) Line: 386.2658 Text Me: 386.855.8758 Toll Free: 888.388.7571 ext. 102 Fax: 888.388.7572 From criley at dpspa.com Tue Dec 3 12:35:34 2019 From: criley at dpspa.com (Charles Riley) Date: Tue, 3 Dec 2019 13:35:34 -0500 Subject: [Histonet] AED machines Message-ID: Doesn anyone know if there are any US regulations (OSHA, CLIA, CAP, DE state) that require pathology practices to have an AED machine on site? Especially if we are seeing patients and performing procedures on them in the lab. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From relia1 at earthlink.net Tue Dec 3 14:03:06 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 3 Dec 2019 15:03:06 -0500 Subject: [Histonet] RELIA Histology Careers Bulletin. Exciting Opportunities. Start now or after the holidays! Message-ID: <000001d5aa14$ae596660$0b0c3320$@earthlink.net> Hello Histopeeps, I hope you are enjoying a wonderful holiday season and are looking forward to an exciting 2020! I have exciting opportunities in the following areas: Leadership Opportunities: ? Tennessee ? Illinois ? Massachusetts ? California IHC Opportunities: ? California ? Texas ? Illinois ? Massachusetts Mohs Tech Opportunities ? Florida ? Alabama Histotechnician/ Technologist Opportunities: ? California ? Kansas ? Texas ? North Carolina ? South Carolina ? New York ? Wisconsin If you are looking immediately or interested in ANY of the these areas call my cell at 407-353-5070!! Or drop me a quick email at relia1 at earthlink.net My clients say you can start NOW or AFTER the holidays YOUR CHOICE! Happy Holidays !!!! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From regan.fulton at gmail.com Tue Dec 3 16:39:12 2019 From: regan.fulton at gmail.com (Fulton Regan) Date: Tue, 3 Dec 2019 14:39:12 -0800 Subject: [Histonet] Superfrost adhesion problems? Message-ID: <77F4E9ED-ABF8-4F36-AAFE-7FFDAE31BBF1@gmail.com> Histonetters, We have heard some anecdotal reports of a recent decrease in the adhesive performance of Superfrost slides, with tissue falling off during IHC staining on automated instruments. We use a lot of these slides and we may have experienced this problem. Has anyone else encountered this problem recently? Thanks. Regan Fulton, M.D., Ph.D. Array Science, LLC 475 Gate 5 Road, #102 Sausalito, CA 94965 www.arrayscience.com From robinsoc at mercyhealth.com Wed Dec 4 07:11:57 2019 From: robinsoc at mercyhealth.com (Cynthia Robinson) Date: Wed, 4 Dec 2019 13:11:57 +0000 Subject: [Histonet] [External] Superfrost adhesion problems? In-Reply-To: <77F4E9ED-ABF8-4F36-AAFE-7FFDAE31BBF1@gmail.com> References: <77F4E9ED-ABF8-4F36-AAFE-7FFDAE31BBF1@gmail.com> Message-ID: We have seen surface tension issues with staining on slides. We had several lots and were able to remove the bad lots from our stock and that has helped. Cindi Robinson HT(ASCP) Histology Lead MercyOne Siouxland Medical Center Dunes Medical Laboratories 101 Tower Road, Suite 220 Dakota Dunes SD 57049 ________________________________ From: Fulton Regan via Histonet Sent: Tuesday, December 3, 2019 4:39 PM To: histonet at lists.utsouthwestern.edu Subject: [External] [Histonet] Superfrost adhesion problems? Warning: This email originated from the Internet! DO NOT CLICK links if the sender is unknown, and NEVER provide your password. Histonetters, We have heard some anecdotal reports of a recent decrease in the adhesive performance of Superfrost slides, with tissue falling off during IHC staining on automated instruments. We use a lot of these slides and we may have experienced this problem. Has anyone else encountered this problem recently? Thanks. Regan Fulton, M.D., Ph.D. Array Science, LLC 475 Gate 5 Road, #102 Sausalito, CA 94965 www.arrayscience.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From deborah-heims at uiowa.edu Wed Dec 4 10:33:52 2019 From: deborah-heims at uiowa.edu (Heims, Deborah A) Date: Wed, 4 Dec 2019 16:33:52 +0000 Subject: [Histonet] Goat synovium Message-ID: <88fb49a59806498d87ce2089b40fd774@uiowa.edu> Hello: I would like to get some help on an auto tissue processing protocol for goat synovium. Some are thicker than others. I have never used goat so was looking for some help? Thank you Debbie ________________________________ Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521 and is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you are not the intended recipient, any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately and delete or destroy all copies of the original message and attachments thereto. Email sent to or from UI Health Care may be retained as required by law or regulation. Thank you. ________________________________ From Scott.Lindrud at carrishealth.com Thu Dec 5 09:19:39 2019 From: Scott.Lindrud at carrishealth.com (Lindrud, Scott) Date: Thu, 5 Dec 2019 15:19:39 +0000 Subject: [Histonet] Weekend Coverage for Small Pathology Labs Message-ID: Hi Histonet, I apologize if this is being re-posted but I did not see this message on the daily Histonet Digest so I'm not sure if it ever got posted..... I was wondering if anyone would be willing to share their experience with weekend Histology coverage for smaller Pathology labs? When I say small, I'm saying about 30,000-33,000 blocks per year (avg 130-140/day) with automated IHC and special staining. Our lab is in a rural area where all but one of the histotechs live between 25-50 miles away. Winter weather has played a role in numerous instances of trying to get a histotech to the lab on a bad weather Saturday. Our lab currently has a histotech come in on Saturday morning to set up a gross for about 16-20 cases which will be put on processing run to come off on Monday morning. The majority of the cases are from colonoscopies, colposcopies, skin excisions, gallbladder/appendix, and placentas. I'm asking this to see if there is a necessity for the Saturday coverage for a lab our size. If the industry standard is that most labs of smaller size have some sort of Saturday histology coverage, then we're good to go. But if most smaller labs aren't providing some sort of weekend coverage, then I feel more confident talking to management for our histotechs about the necessity of providing the Saturday coverage. I just don't know what other smaller labs are doing. Thanks for any opinions or information! Scott A. Lindrud, MLSCM(ASCP)CTCM | Histopathology Technical Specialist Phone: 320-231-4406 Fax: 320-231-4305 scott.lindrud at carrishealth.com Confidentiality Notice: This e-mail and any attachment may contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this transmission in error, please notify the sender immediately, reply to this transmission, or contact the CentraCare Information Systems Network Security staff by calling the IS Help Desk for assistance at 320-251-2700, ext. 54540, and delete these documents. From tbraud at holyredeemer.com Thu Dec 5 12:26:41 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 5 Dec 2019 18:26:41 +0000 Subject: [Histonet] weekends in the small lab Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B6A7EE@HRHEX02-HOS.holyredeemer.local> In my experience in 2 small hospital labs, weekend coverage is just not worth it. If it is a major surgery, then the patient will be recuperating and if it is just a biopsy, the ordering physician is rarely available to correlate the Pathology findings with the clinical findings. Most tell the patient that they will get their results in a week or two. The only exception that I have encountered was if you process non-gyn cytology. On rare occasions, we found the need to process a fluid in order to look for organisms or to send for flow, though we seldom did more than one per year. For those occasions, we usually just had someone volunteer to come in to process it in order to save our weekends free. Generally, at both hospital labs, we were closed when surgery was closed, even on 3 day weekends and we've never had trouble or complaints. The weekend cases you describe are not a rush. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Weekend Coverage for Small Pathology Labs (Lindrud, Scott) Message: 1 Date: Thu, 5 Dec 2019 15:19:39 +0000 From: "Lindrud, Scott" Subject: [Histonet] Weekend Coverage for Small Pathology Labs Hi Histonet, I apologize if this is being re-posted but I did not see this message on the daily Histonet Digest so I'm not sure if it ever got posted..... I was wondering if anyone would be willing to share their experience with weekend Histology coverage for smaller Pathology labs? When I say small, I'm saying about 30,000-33,000 blocks per year (avg 130-140/day) with automated IHC and special staining. Our lab is in a rural area where all but one of the histotechs live between 25-50 miles away. Winter weather has played a role in numerous instances of trying to get a histotech to the lab on a bad weather Saturday. Our lab currently has a histotech come in on Saturday morning to set up a gross for about 16-20 cases which will be put on processing run to come off on Monday morning. The majority of the cases are from colonoscopies, colposcopies, skin excisions, gallbladder/appendix, and placentas. I'm asking this to see if there is a necessity for the Saturday coverage for a lab our size. If the industry standard is that most labs of smaller size have some sort of Saturday histology coverage, then we're good to go. But if most smaller labs aren't providing some sort of weekend coverage, then I feel more confident talking to management for our histotechs about the necessity of providing the Saturday coverage. I just don't know what other smaller labs are doing. Thanks for any opinions or information! Scott A. Lindrud, MLSCM(ASCP)CTCM | Histopathology Technical Specialist Phone: 320-231-4406 Fax: 320-231-4305 scott.lindrud at carrishealth.com Confidentiality Notice: This e-mail and any attachment may contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this transmission in error, please notify the sender immediately, reply to this transmission, or contact the CentraCare Information Systems Network Security staff by calling the IS Help Desk for assistance at 320-251-2700, ext. 54540, and delete these documents. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 193, Issue 3 **************************************** From rsrichmond at gmail.com Thu Dec 5 12:38:15 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 5 Dec 2019 13:38:15 -0500 Subject: [Histonet] Weekend Coverage for Small Pathology Labs In-Reply-To: References: Message-ID: Scott A. Lindrud, MLSCM(ASCP)CTCM, Histopathology Technical Specialist at Carris Health in central Minnesota (apparently not part of the Mayo Clinic system) asks: >>I was wondering if anyone would be willing to share their experience with weekend Histology coverage for smaller Pathology labs? When I say small, I'm saying about 30,000-33,000 blocks per year (avg 130-140/day) with automated IHC and special staining. Our lab is in a rural area where all but one of the histotechs live between 25-50 miles away. Winter weather has played a role in numerous instances of trying to get a histotech to the lab on a bad weather Saturday. -- Our lab currently has a histotech come in on Saturday morning to set up a gross for about 16-20 cases which will be put on processing run to come off on Monday morning. The majority of the cases are from colonoscopies, colposcopies, skin excisions, gallbladder/appendix, and placentas. -- I'm asking this to see if there is a necessity for the Saturday coverage for a lab our size. If the industry standard is that most labs of smaller size have some sort of Saturday histology coverage, then we're good to go. But if most smaller labs aren't providing some sort of weekend coverage, then I feel more confident talking to management for our histotechs about the necessity of providing the Saturday coverage. I just don't know what other smaller labs are doing.<< Now retired at age 80, I did locum tenens coverage of a large number of rural pathology practices in the southeastern US and adjacent states. I'm not sure I saw any of these labs providing Saturday coverage after the 1980s, though many of them did before that. "The majority of the cases are from colonoscopies, colposcopies, skin excisions, gallbladder/appendix, and placentas." I don't see why it's necessary to cut any of these on Saturday. Bob Richmond Samurai Pathologist Maryville TN From patpxs at gmail.com Thu Dec 5 13:57:47 2019 From: patpxs at gmail.com (P Sicurello) Date: Thu, 5 Dec 2019 11:57:47 -0800 Subject: [Histonet] Leica Peloris Question Message-ID: Hello Listers, Does anyone know why the recommended processing for the Peloris is 47o C? I was taught (back in the dark ages) that high temperatures during processing were not good for the tissue. With the exception of the paraffins, the processing temperatures were usually around 27 - 37o C. 47o C seems like it would sort of cook the tissue bits (especially the tiny biopsies, like g.i.) Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From garreyf at gmail.com Fri Dec 6 18:01:30 2019 From: garreyf at gmail.com (Garrey Faller) Date: Fri, 6 Dec 2019 19:01:30 -0500 Subject: [Histonet] weekends in the small lab In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F801C1B6A7EE@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F801C1B6A7EE@HRHEX02-HOS.holyredeemer.local> Message-ID: Agree. As a pathologist, I?ve benefited from weekend coverage at a much smaller Hospital lab. Now am at a much busier lab and we don?t have weekend coverage . Would I like it? Yes. But we function fine without it. One thing to always consider is cap/Asco time fixation guidelines for breast biopsies. Having a weekend run gives you more flexibility. G Sent from my iPhone > On Dec 5, 2019, at 1:40 PM, Terri Braud via Histonet wrote: > > ?In my experience in 2 small hospital labs, weekend coverage is just not worth it. If it is a major surgery, then the patient will be recuperating and if it is just a biopsy, the ordering physician is rarely available to correlate the Pathology findings with the clinical findings. Most tell the patient that they will get their results in a week or two. > The only exception that I have encountered was if you process non-gyn cytology. On rare occasions, we found the need to process a fluid in order to look for organisms or to send for flow, though we seldom did more than one per year. For those occasions, we usually just had someone volunteer to come in to process it in order to save our weekends free. > Generally, at both hospital labs, we were closed when surgery was closed, even on 3 day weekends and we've never had trouble or complaints. The weekend cases you describe are not a rush. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > > Today's Topics: > 1. Weekend Coverage for Small Pathology Labs (Lindrud, Scott) > Message: 1 > Date: Thu, 5 Dec 2019 15:19:39 +0000 > From: "Lindrud, Scott" > Subject: [Histonet] Weekend Coverage for Small Pathology Labs > > Hi Histonet, > I apologize if this is being re-posted but I did not see this message on the daily Histonet Digest so I'm not sure if it ever got posted..... > I was wondering if anyone would be willing to share their experience with weekend Histology coverage for smaller Pathology labs? When I say small, I'm saying about 30,000-33,000 blocks per year (avg 130-140/day) with automated IHC and special staining. Our lab is in a rural area where all but one of the histotechs live between 25-50 miles away. Winter weather has played a role in numerous instances of trying to get a histotech to the lab on a bad weather Saturday. > > Our lab currently has a histotech come in on Saturday morning to set up a gross for about 16-20 cases which will be put on processing run to come off on Monday morning. The majority of the cases are from colonoscopies, colposcopies, skin excisions, gallbladder/appendix, and placentas. > > I'm asking this to see if there is a necessity for the Saturday coverage for a lab our size. If the industry standard is that most labs of smaller size have some sort of Saturday histology coverage, then we're good to go. But if most smaller labs aren't providing some sort of weekend coverage, then I feel more confident talking to management for our histotechs about the necessity of providing the Saturday coverage. I just don't know what other smaller labs are doing. > > Thanks for any opinions or information! > > Scott A. Lindrud, MLSCM(ASCP)CTCM | Histopathology Technical Specialist > Phone: 320-231-4406 > Fax: 320-231-4305 > scott.lindrud at carrishealth.com > > > > Confidentiality Notice: This e-mail and any attachment may contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this transmission in error, please notify the sender immediately, reply to this transmission, or contact the CentraCare Information Systems Network Security staff by calling the IS Help Desk for assistance at 320-251-2700, ext. 54540, and delete these documents. > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 193, Issue 3 > **************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Mon Dec 9 08:38:56 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 9 Dec 2019 14:38:56 +0000 Subject: [Histonet] weekends in the small lab In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F801C1B6A7EE@HRHEX02-HOS.holyredeemer.local> Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B6ADB3@HRHEX02-HOS.holyredeemer.local> Garrey brings up an excellent point, cautioning carefulness when dealing with breast fixation times over the weekend. When we receive breast biopsies on Thursday, that do not have enough fixation time to load Thursday evening, we run them Friday morning as soon as a processor becomes available. For breast cases received on Friday, they are processed as a routine specimen, coming off on Monday. For the rare occasion that we have a Friday breast received before a 3 day weekend, we will ask for a volunteer to come in and remove it from a routine schedule before the 72 hour fixation time is exceeded. We just let the blocks sit at room temp in paraffin until embedded. We have been operating this way for years with no problem. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: Garrey Faller [mailto:garreyf at gmail.com] Sent: Friday, December 06, 2019 7:02 PM To: Terri Braud Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] weekends in the small lab CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links or opening attachments. Agree. As a pathologist, I?ve benefited from weekend coverage at a much smaller Hospital lab. Now am at a much busier lab and we don?t have weekend coverage . Would I like it? Yes. But we function fine without it. One thing to always consider is cap/Asco time fixation guidelines for breast biopsies. Having a weekend run gives you more flexibility. G Sent from my iPhone > On Dec 5, 2019, at 1:40 PM, Terri Braud via Histonet wrote: > > ?In my experience in 2 small hospital labs, weekend coverage is just not worth it. If it is a major surgery, then the patient will be recuperating and if it is just a biopsy, the ordering physician is rarely available to correlate the Pathology findings with the clinical findings. Most tell the patient that they will get their results in a week or two. > The only exception that I have encountered was if you process non-gyn cytology. On rare occasions, we found the need to process a fluid in order to look for organisms or to send for flow, though we seldom did more than one per year. For those occasions, we usually just had someone volunteer to come in to process it in order to save our weekends free. > Generally, at both hospital labs, we were closed when surgery was closed, even on 3 day weekends and we've never had trouble or complaints. The weekend cases you describe are not a rush. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > > Today's Topics: > 1. Weekend Coverage for Small Pathology Labs (Lindrud, Scott) > Message: 1 > Date: Thu, 5 Dec 2019 15:19:39 +0000 > From: "Lindrud, Scott" > Subject: [Histonet] Weekend Coverage for Small Pathology Labs > > Hi Histonet, > I apologize if this is being re-posted but I did not see this message on the daily Histonet Digest so I'm not sure if it ever got posted..... > I was wondering if anyone would be willing to share their experience with weekend Histology coverage for smaller Pathology labs? When I say small, I'm saying about 30,000-33,000 blocks per year (avg 130-140/day) with automated IHC and special staining. Our lab is in a rural area where all but one of the histotechs live between 25-50 miles away. Winter weather has played a role in numerous instances of trying to get a histotech to the lab on a bad weather Saturday. > > Our lab currently has a histotech come in on Saturday morning to set up a gross for about 16-20 cases which will be put on processing run to come off on Monday morning. The majority of the cases are from colonoscopies, colposcopies, skin excisions, gallbladder/appendix, and placentas. > > I'm asking this to see if there is a necessity for the Saturday coverage for a lab our size. If the industry standard is that most labs of smaller size have some sort of Saturday histology coverage, then we're good to go. But if most smaller labs aren't providing some sort of weekend coverage, then I feel more confident talking to management for our histotechs about the necessity of providing the Saturday coverage. I just don't know what other smaller labs are doing. > > Thanks for any opinions or information! > > Scott A. Lindrud, MLSCM(ASCP)CTCM | Histopathology Technical Specialist > Phone: 320-231-4406 > Fax: 320-231-4305 > scott.lindrud at carrishealth.com > > > > Confidentiality Notice: This e-mail and any attachment may contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this transmission in error, please notify the sender immediately, reply to this transmission, or contact the CentraCare Information Systems Network Security staff by calling the IS Help Desk for assistance at 320-251-2700, ext. 54540, and delete these documents. > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 193, Issue 3 > **************************************** > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at dpspa.com Mon Dec 9 13:31:31 2019 From: criley at dpspa.com (Charles Riley) Date: Mon, 9 Dec 2019 14:31:31 -0500 Subject: [Histonet] Tissue based PCR FISH and SISH platforms Message-ID: Is anyone aware of a platform that can perform all three of the testing methods in my subject line besides the Biogenex platforms? If not, can anyone recommend a good combination of machines that can perform all three tests ? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From Timothy.Morken at ucsf.edu Wed Dec 11 11:02:58 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 11 Dec 2019 17:02:58 +0000 Subject: [Histonet] SECURE: Please send block to EM lab Message-ID: This is a secure message. Click here https://pe-002e8f01.gslb.pphosted.com/formpostdir/securereader?id=TSHBjjhKZJQbAmmmM9ouoE388nfx0p00&brand=66d9f965 by 2019-12-18 09:03 PST to read your message. After that, open the attachment. -------------- next part -------------- [Image?c=logo&b=1&i=2&rnd=3.1510941425865] [Image?c=lock&b=1&rnd=8.74012900646182] Click to read message If you do not see a button or clicking the button yields no action, please click the link in the message. [1]More Info Disclaimer: This email and its content are confidential and intended solely for the use of the addressee. Please notify the sender if you have received this email in error or simply delete it. Secured by Proofpoint Encryption, Copyright ? 2009-2019 Proofpoint, Inc. All rights reserved. References 1. https://it.ucsf.edu/services/secure-email/additional/secure-email-recipient-help From Timothy.Morken at ucsf.edu Wed Dec 11 11:14:15 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 11 Dec 2019 17:14:15 +0000 Subject: [Histonet] Recall: SECURE: Please send block to EM lab Message-ID: This is a secure message. Click here https://pe-002e8f01.gslb.pphosted.com/formpostdir/securereader?id=1VTCLvZhK2OwIXUVqkgYRCeLHWKdc-FL&brand=66d9f965 by 2019-12-18 09:14 PST to read your message. After that, open the attachment. -------------- next part -------------- [Image?c=logo&b=1&i=2&rnd=3.1510941425865] [Image?c=lock&b=1&rnd=8.74012900646182] Click to read message If you do not see a button or clicking the button yields no action, please click the link in the message. [1]More Info Disclaimer: This email and its content are confidential and intended solely for the use of the addressee. Please notify the sender if you have received this email in error or simply delete it. Secured by Proofpoint Encryption, Copyright ? 2009-2019 Proofpoint, Inc. All rights reserved. References 1. https://it.ucsf.edu/services/secure-email/additional/secure-email-recipient-help From amurvosh at advancederm.net Wed Dec 11 11:48:35 2019 From: amurvosh at advancederm.net (Anne Murvosh) Date: Wed, 11 Dec 2019 17:48:35 +0000 Subject: [Histonet] Xylene and Formalin monitoring Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7CA2F76@Exchange.Advancederm.net> Just need a refresher on formalin and xylene monitoring regulations. Do we still have to do a 15 minute and 8 hour testing on both xylene and formalin, or can we just do the 8 hour. I wasn't sure if the regulations have changed or not. Thanks Anne From tabbott at pennstatehealth.psu.edu Wed Dec 11 14:44:52 2019 From: tabbott at pennstatehealth.psu.edu (Abbott, Tanya) Date: Wed, 11 Dec 2019 20:44:52 +0000 Subject: [Histonet] Job posting Message-ID: <04108f5235394a8ca689731dd92fa597@pennstatehealth.psu.edu> St. Joseph Hospital in Reading, PA has an immediate opening for a Full-time Histotechnologist. JOB SUMMARY: The Histotechnologist at St. Joseph Hospital is responsible for performing technical duties in preparation of surgical specimens for microscopic examination. RESPONSIBILITIES: Receive and accession tissue specimens and prepare specimens for processing. Cut thin sections and stain according to established procedures for routine, special stains, immunohistochemistry and coverslip and label finished slides for the pathologists review. Assist the pathologist with cutting and staining of tissues for frozen sections. Maintain equipment, instruments and cleanliness of working area and maintain a safe, clean work environment including proper labeling and storage of chemicals and specimens (and disposal of same). Prepares written documentation as required by regulatory agencies, the profession and the department. Regulatory agencies include, but are not limited to, FDA, JCAHO, CAP, CLIA, OSHA and the state in which this facility is located. MINIMUM QUALIFICATIONS: Associates degree required At least two years on-the-job training or experience is required Experience with personal computers is required PREFERRED QUALIFICATIONS: Bachelors degree preferred Certified as HTL/HT (ASCP) or equivalent preferred or eligible for certification as HTL/HT (ASCP) St. Joseph Hospital offers an exceptional benefit package including but not limited to: * Health, Vision, and Dental plans effective first day of hire * Wellness programs * Employee discounts * Generous Paid time off for work/life balance About St. Joseph Hospital: Located in the heart of Berks County in Reading, PA, St. Joseph Medical Center first opened its doors in 1873. The new 204 bed state of the art hospital, located on Bernville Road, opened in 2004 and in 2015 became the first hospital to join the Penn State Health organization. Penn State Health St. Joseph is dedicated to the health, education and wellness of the community. To apply online, visit our career site: careers.pennstatehealth.org Job #16924 Thank you! Tanya G. Abbott Pathology Manager PennState Health St. Joseph Reading Pennsylvania tabbott at pennstatehealth.psu.edu 610-378-2635 From akemiat3377 at gmail.com Thu Dec 12 06:12:50 2019 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Thu, 12 Dec 2019 05:12:50 -0700 Subject: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel Message-ID: Good morning histopeeps: We recently brought on an Ophthalmology Pathologist from MD Anderson and they use histogel for orienting their thin eye specimens and need a microwave to heat it up. We do not have a microwave in the lab for their use but I had ordered one for them. I just found out they used the microwave in our lab lounge to heat up histonet which was opened and being stored in our gross area refrigerator. Do any of you know the CAP regulation against use of laboratory reagents being used in a microwave being used for food consumption? I ended up removing the microwave they used and put it in the histology lab. Our Director wants me to write a formal letter to submit to him. Thank you in advance for your impute! Akemi Allison, BS, HT/HTL (ASCP) From akemiat3377 at gmail.com Thu Dec 12 07:32:10 2019 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Thu, 12 Dec 2019 06:32:10 -0700 Subject: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel In-Reply-To: References: Message-ID: <87FF1E7E-84CA-4A79-82E5-D0497261E43A@gmail.com> Went on line to get MSDS regarding HistoGel. Here?s the link. https://www.medline.com/media/catalog/Docs/MSDS/MSD_SDSD91968.pdf Happy eating from a microwave which has been used at MD Anderson for HistGel! Best regards, Akemi > On Dec 12, 2019, at 5:18 AM, Kathleen S Cormier wrote: > > Can I share that we had a pathologist (back in the day) use our lab microwave to re-heat his coffee? It had Schiff?s reagent stains all over the inside of it. Some people really don?t have any common sense? > > > Kathy Cormier > Core Leader > Hope Babette Tang Histology Facility > Koch Institute for Integrative Cancer Research at MIT > 500 Main Street, 76-182 > Cambridge, MA 02139 > 617-258-8183 > cormier at mit.edu > https://ki.mit.edu/sbc/histology > > > > > > > > > >> On Dec 12, 2019, at 7:12 AM, Eileen Akemi Allison via Histonet > wrote: >> >> Good morning histopeeps: >> >> We recently brought on an Ophthalmology Pathologist from MD Anderson and they use histogel for orienting their thin eye specimens and need a microwave to heat it up. We do not have a microwave in the lab for their use but I had ordered one for them. I just found out they used the microwave in our lab lounge to heat up histonet which was opened and being stored in our gross area refrigerator. >> >> Do any of you know the CAP regulation against use of laboratory reagents being used in a microwave being used for food consumption? I ended up removing the microwave they used and put it in the histology lab. Our Director wants me to write a formal letter to submit to him. >> >> Thank you in advance for your impute! >> >> Akemi Allison, BS, HT/HTL (ASCP) >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From john.garratt at ciqc.ca Thu Dec 12 07:51:10 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 12 Dec 2019 13:51:10 +0000 Subject: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel In-Reply-To: <87FF1E7E-84CA-4A79-82E5-D0497261E43A@gmail.com> References: <87FF1E7E-84CA-4A79-82E5-D0497261E43A@gmail.com> Message-ID: NEVER use a microwave to heat up Histogel regardless of ithe microwaves pedigree, especially for cytology specimens. There is a tendency to overheat the gel and thereby compromise the specimen, especially for IHC. Use a warming block where the temperature can be accurately controlled. John Sent from ProtonMail Mobile On Thu, Dec 12, 2019 at 5:32 AM, Eileen Akemi Allison via Histonet wrote: > Went on line to get MSDS regarding HistoGel. Here?s the link. https://www.medline.com/media/catalog/Docs/MSDS/MSD_SDSD91968.pdf > > Happy eating from a microwave which has been used at MD Anderson for HistGel! > > Best regards, > Akemi > >> On Dec 12, 2019, at 5:18 AM, Kathleen S Cormier wrote: >> >> Can I share that we had a pathologist (back in the day) use our lab microwave to re-heat his coffee? It had Schiff?s reagent stains all over the inside of it. Some people really don?t have any common sense? >> >> >> Kathy Cormier >> Core Leader >> Hope Babette Tang Histology Facility >> Koch Institute for Integrative Cancer Research at MIT >> 500 Main Street, 76-182 >> Cambridge, MA 02139 >> 617-258-8183 >> cormier at mit.edu >> https://ki.mit.edu/sbc/histology >> >> >> >> >> >> >> >> >> >>> On Dec 12, 2019, at 7:12 AM, Eileen Akemi Allison via Histonet > wrote: >>> >>> Good morning histopeeps: >>> >>> We recently brought on an Ophthalmology Pathologist from MD Anderson and they use histogel for orienting their thin eye specimens and need a microwave to heat it up. We do not have a microwave in the lab for their use but I had ordered one for them. I just found out they used the microwave in our lab lounge to heat up histonet which was opened and being stored in our gross area refrigerator. >>> >>> Do any of you know the CAP regulation against use of laboratory reagents being used in a microwave being used for food consumption? I ended up removing the microwave they used and put it in the histology lab. Our Director wants me to write a formal letter to submit to him. >>> >>> Thank you in advance for your impute! >>> >>> Akemi Allison, BS, HT/HTL (ASCP) >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at dpspa.com Thu Dec 12 07:52:55 2019 From: criley at dpspa.com (Charles Riley) Date: Thu, 12 Dec 2019 08:52:55 -0500 Subject: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel In-Reply-To: <87FF1E7E-84CA-4A79-82E5-D0497261E43A@gmail.com> References: <87FF1E7E-84CA-4A79-82E5-D0497261E43A@gmail.com> Message-ID: There is no direct CAP regulation regarding the use of microwaves concerning food and chemical use. However, ANP.29430 says that microwaves used in the histology lab need to be under a fume hood to capture any vented chemical fumes. Also, I am not sure what the actual record is but I am sure there is an OSHA regulation that is part of the Chemical exposure regulations that states microwaves used in laboratories can not be used for both food and chemical processing. On Thu, Dec 12, 2019 at 8:37 AM Eileen Akemi Allison via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Went on line to get MSDS regarding HistoGel. Here?s the link. > https://www.medline.com/media/catalog/Docs/MSDS/MSD_SDSD91968.pdf < > https://www.medline.com/media/catalog/Docs/MSDS/MSD_SDSD91968.pdf> > > Happy eating from a microwave which has been used at MD Anderson for > HistGel! > > Best regards, > Akemi > > > On Dec 12, 2019, at 5:18 AM, Kathleen S Cormier wrote: > > > > Can I share that we had a pathologist (back in the day) use our lab > microwave to re-heat his coffee? It had Schiff?s reagent stains all over > the inside of it. Some people really don?t have any common sense? > > > > > > Kathy Cormier > > Core Leader > > Hope Babette Tang Histology Facility > > Koch Institute for Integrative Cancer Research at MIT > > 500 Main Street, 76-182 > > Cambridge, MA 02139 > > 617-258-8183 > > cormier at mit.edu > > https://ki.mit.edu/sbc/histology > > > > > > > > > > > > > > > > > > > >> On Dec 12, 2019, at 7:12 AM, Eileen Akemi Allison via Histonet < > histonet at lists.utsouthwestern.edu histonet at lists.utsouthwestern.edu>> wrote: > >> > >> Good morning histopeeps: > >> > >> We recently brought on an Ophthalmology Pathologist from MD Anderson > and they use histogel for orienting their thin eye specimens and need a > microwave to heat it up. We do not have a microwave in the lab for their > use but I had ordered one for them. I just found out they used the > microwave in our lab lounge to heat up histonet which was opened and being > stored in our gross area refrigerator. > >> > >> Do any of you know the CAP regulation against use of laboratory > reagents being used in a microwave being used for food consumption? I > ended up removing the microwave they used and put it in the histology lab. > Our Director wants me to write a formal letter to submit to him. > >> > >> Thank you in advance for your impute! > >> > >> Akemi Allison, BS, HT/HTL (ASCP) > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet at lists.utsouthwestern.edu Histonet at lists.utsouthwestern.edu> > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From john.garratt at ciqc.ca Thu Dec 12 08:32:32 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 12 Dec 2019 14:32:32 +0000 Subject: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel In-Reply-To: References: <87FF1E7E-84CA-4A79-82E5-D0497261E43A@gmail.com> Message-ID: <2N3Sxa6Yauf0y4WZyWxF_EFuIf9k4Quq7f-DGmUyJuZM80Wxax3vqvbOjePJk9G9KPlrjfP8UfIW2DyVgiuH7Z35JJaPRweET3YVONjhCXQ=@ciqc.ca> With regard to microwaves themselves you may find requirements related to monitoring for microwave radiation leakage in your local regulations and the recording of such inspections. It is a radiation device. Trouble with your cytology IHC? Check how your cell blocks are being prepared and get rid of that microwave. John Sent from ProtonMail Mobile On Thu, Dec 12, 2019 at 5:52 AM, Charles Riley via Histonet wrote: > There is no direct CAP regulation regarding the use of microwaves > concerning food and chemical use. However, ANP.29430 says that microwaves > used in the histology lab need to be under a fume hood to capture any > vented chemical fumes. Also, I am not sure what the actual record is but > I am sure there is an OSHA regulation that is part of the Chemical exposure > regulations that states microwaves used in laboratories can not be used for > both food and chemical processing. > > On Thu, Dec 12, 2019 at 8:37 AM Eileen Akemi Allison via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Went on line to get MSDS regarding HistoGel. Here?s the link. >> https://www.medline.com/media/catalog/Docs/MSDS/MSD_SDSD91968.pdf < >> https://www.medline.com/media/catalog/Docs/MSDS/MSD_SDSD91968.pdf> >> >> Happy eating from a microwave which has been used at MD Anderson for >> HistGel! >> >> Best regards, >> Akemi >> >> > On Dec 12, 2019, at 5:18 AM, Kathleen S Cormier wrote: >> > >> > Can I share that we had a pathologist (back in the day) use our lab >> microwave to re-heat his coffee? It had Schiff?s reagent stains all over >> the inside of it. Some people really don?t have any common sense? >> > >> > >> > Kathy Cormier >> > Core Leader >> > Hope Babette Tang Histology Facility >> > Koch Institute for Integrative Cancer Research at MIT >> > 500 Main Street, 76-182 >> > Cambridge, MA 02139 >> > 617-258-8183 >> > cormier at mit.edu >> > https://ki.mit.edu/sbc/histology >> > >> > >> > >> > >> > >> > >> > >> > >> > >> >> On Dec 12, 2019, at 7:12 AM, Eileen Akemi Allison via Histonet < >> histonet at lists.utsouthwestern.edu > histonet at lists.utsouthwestern.edu>> wrote: >> >> >> >> Good morning histopeeps: >> >> >> >> We recently brought on an Ophthalmology Pathologist from MD Anderson >> and they use histogel for orienting their thin eye specimens and need a >> microwave to heat it up. We do not have a microwave in the lab for their >> use but I had ordered one for them. I just found out they used the >> microwave in our lab lounge to heat up histonet which was opened and being >> stored in our gross area refrigerator. >> >> >> >> Do any of you know the CAP regulation against use of laboratory >> reagents being used in a microwave being used for food consumption? I >> ended up removing the microwave they used and put it in the histology lab. >> Our Director wants me to write a formal letter to submit to him. >> >> >> >> Thank you in advance for your impute! >> >> >> >> Akemi Allison, BS, HT/HTL (ASCP) >> >> >> >> >> >> _______________________________________________ >> >> Histonet mailing list >> >> Histonet at lists.utsouthwestern.edu > Histonet at lists.utsouthwestern.edu> >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CIngles at uwhealth.org Thu Dec 12 09:22:40 2019 From: CIngles at uwhealth.org (Ingles Claire) Date: Thu, 12 Dec 2019 15:22:40 +0000 Subject: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel In-Reply-To: References: Message-ID: OMG!!! really?!? OSHA, Joint Commission, CAP, ANY regulatory agency. Give them the lounge microwave and get a new one for your break room immediately!! Fumes, contamination on the outside of whatever it's in, etc. Not even mentioning if it gets spilled. But then again, our docs like to store Botox in our lunch fridge once in a while... *sigh* Claire ________________________________ From: Eileen Akemi Allison via Histonet Sent: Thursday, December 12, 2019 6:12 AM To: Histonet Subject: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Good morning histopeeps: We recently brought on an Ophthalmology Pathologist from MD Anderson and they use histogel for orienting their thin eye specimens and need a microwave to heat it up. We do not have a microwave in the lab for their use but I had ordered one for them. I just found out they used the microwave in our lab lounge to heat up histonet which was opened and being stored in our gross area refrigerator. Do any of you know the CAP regulation against use of laboratory reagents being used in a microwave being used for food consumption? I ended up removing the microwave they used and put it in the histology lab. Our Director wants me to write a formal letter to submit to him. Thank you in advance for your impute! Akemi Allison, BS, HT/HTL (ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz at premierlab.com Thu Dec 12 10:27:26 2019 From: liz at premierlab.com (Liz Chlipala) Date: Thu, 12 Dec 2019 16:27:26 +0000 Subject: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel In-Reply-To: <87FF1E7E-84CA-4A79-82E5-D0497261E43A@gmail.com> References: <87FF1E7E-84CA-4A79-82E5-D0497261E43A@gmail.com> Message-ID: You don?t need to heat up histogel in a microwave its quicker that way but if you set an oven at around 65 to 70 it will melt in around 20 minutes or so, that?s what we do. It can get messy in a microwave. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Eileen Akemi Allison via Histonet Sent: Thursday, December 12, 2019 6:32 AM To: Kathleen S Cormier Cc: Histonet Subject: Re: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel Went on line to get MSDS regarding HistoGel. Here?s the link. https://www.medline.com/media/catalog/Docs/MSDS/MSD_SDSD91968.pdf Happy eating from a microwave which has been used at MD Anderson for HistGel! Best regards, Akemi > On Dec 12, 2019, at 5:18 AM, Kathleen S Cormier wrote: > > Can I share that we had a pathologist (back in the day) use our lab microwave to re-heat his coffee? It had Schiff?s reagent stains all over the inside of it. Some people really don?t have any common sense? > > > Kathy Cormier > Core Leader > Hope Babette Tang Histology Facility > Koch Institute for Integrative Cancer Research at MIT > 500 Main Street, 76-182 > Cambridge, MA 02139 > 617-258-8183 > cormier at mit.edu > https://ki.mit.edu/sbc/histology > > > > > > > > > >> On Dec 12, 2019, at 7:12 AM, Eileen Akemi Allison via Histonet > wrote: >> >> Good morning histopeeps: >> >> We recently brought on an Ophthalmology Pathologist from MD Anderson and they use histogel for orienting their thin eye specimens and need a microwave to heat it up. We do not have a microwave in the lab for their use but I had ordered one for them. I just found out they used the microwave in our lab lounge to heat up histonet which was opened and being stored in our gross area refrigerator. >> >> Do any of you know the CAP regulation against use of laboratory reagents being used in a microwave being used for food consumption? I ended up removing the microwave they used and put it in the histology lab. Our Director wants me to write a formal letter to submit to him. >> >> Thank you in advance for your impute! >> >> Akemi Allison, BS, HT/HTL (ASCP) >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From bcooper at chla.usc.edu Thu Dec 12 13:53:51 2019 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Thu, 12 Dec 2019 19:53:51 +0000 Subject: [Histonet] Cryostat Samples for Molecular Testing Message-ID: Good afternoon Histonet! We would like to know what decontamination procedures you are performing to prevent cross-contamination in between cutting scrolls for frozen samples for molecular testing. We've had a few complaints of trace carryover from one sample to the next. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From amosbrooks at gmail.com Thu Dec 12 17:07:30 2019 From: amosbrooks at gmail.com (Amos Brooks) Date: Thu, 12 Dec 2019 18:07:30 -0500 Subject: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel In-Reply-To: References: Message-ID: > > Hi, There is NO reason to have a microwave in a histology lab. They don't really save any time and cause more problems than they are worth. You won't have to worry about any certifying agencies rulings about microwaves if they aren't there! Just put the Histogel in a rack in a normal convection oven. If your slide drying oven doesn't get that high, your embedding center probably does. Leave it there for an hour and you'll be fine. And good lord don't let them put it in the break room! Amos Message: 2 > Date: Thu, 12 Dec 2019 05:12:50 -0700 > From: Eileen Akemi Allison > To: Histonet > Subject: [Histonet] guidelines for using a microwave designated for > food, but used to heat up histogel > Message-ID: > Content-Type: text/plain; charset=us-ascii > > Good morning histopeeps: > > We recently brought on an Ophthalmology Pathologist from MD Anderson and > they use histogel for orienting their thin eye specimens and need a > microwave to heat it up. We do not have a microwave in the lab for their > use but I had ordered one for them. I just found out they used the > microwave in our lab lounge to heat up histonet which was opened and being > stored in our gross area refrigerator. > > Do any of you know the CAP regulation against use of laboratory reagents > being used in a microwave being used for food consumption? I ended up > removing the microwave they used and put it in the histology lab. Our > Director wants me to write a formal letter to submit to him. > > Thank you in advance for your impute! > > Akemi Allison, BS, HT/HTL (ASCP) > From tbraud at holyredeemer.com Fri Dec 13 08:54:16 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 13 Dec 2019 14:54:16 +0000 Subject: [Histonet] Processing descemet's membrane Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B6B83A@HRHEX02-HOS.holyredeemer.local> Hello Histopeeps - I need your help, please. I need to improve our processing of Descemet's membrane, more specifically keeping track of that little wisp of clear colorless tissue? Do you have any tricks to help keep track of it through processing? Inks? Embedding gel? Anything? I'm open for any ideas to make this process easier. Thanks in advance, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From melissa at alliedsearchpartners.com Mon Dec 16 14:32:57 2019 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Mon, 16 Dec 2019 20:32:57 +0000 Subject: [Histonet] IHC Application Specialist Job in Boston- Message-ID: Hello and Happy Monday, I have an "off the bench" position available for an IHC Application Specialist. Looking for someone with extensive IHC experience and a research emphasis rather than clinical emphasis on experience. Bachelor's Degree at least. Ability to travel throughout Boston and the Northeastern States. 75% travel involved. Must be in Boston area or open to relocate to the Boston area due to the primary customer base in Boston. Message me directly for more details! Melissa Owens Allied Search Partners AN MRINETWORK MEMBER Direct (Call) Line: 386.265.1368 Text Me: 386.855.8758 Toll Free: 888.388.7571 ext. 102 Fax: 888.388.7572 From patpxs at gmail.com Tue Dec 17 13:30:58 2019 From: patpxs at gmail.com (P Sicurello) Date: Tue, 17 Dec 2019 11:30:58 -0800 Subject: [Histonet] Delay in embedding? Message-ID: Good Morning Listers, How many out there will process tissue and then leave the cassettes at room temperature and embed it at a later time (hours or the next day)? Please send me your opinions about doing this. I think it?s a bad idea, others I speak with disagree. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From terrie.dalton at icloud.com Tue Dec 17 14:33:43 2019 From: terrie.dalton at icloud.com (Theresa Dalton) Date: Tue, 17 Dec 2019 15:33:43 -0500 Subject: [Histonet] Delay in embedding? In-Reply-To: References: Message-ID: <693B4D62-DF63-486C-9017-D24B9F190136@icloud.com> We have done this - only on an emergency basis. Sent from my iPhone > On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet wrote: > > ?Good Morning Listers, > > > > How many out there will process tissue and then leave the cassettes at room > temperature and embed it at a later time (hours or the next day)? > > > > Please send me your opinions about doing this. I think it?s a bad idea, > others I speak with disagree. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 9300 Campus Point Drive > > La Jolla, CA 92037 > (P): 858-249-5610 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Tue Dec 17 16:28:13 2019 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue, 17 Dec 2019 22:28:13 +0000 Subject: [Histonet] Delay in embedding? In-Reply-To: <693B4D62-DF63-486C-9017-D24B9F190136@icloud.com> References: <693B4D62-DF63-486C-9017-D24B9F190136@icloud.com> Message-ID: Hi Paula, We routinely do this, especially for our fetal autopsy blocks. We are then able to process and let them set at room temp until we are able to embed and cut. Some cases are more urgent than others so these can be expedited a lot easier since they will only need embedding, sectioning and staining. We also get best usage of our limited processing capabilities. It is more efficient for us. Morphology, staining and immunohistochemistry is not affected. It is better to do this rather than leave them at 64oC plus for extended times prior to embedding since many antigens will be adversely affected. Take home point - do not overcook your tissues. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Theresa Dalton via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 18 December 2019 7:34 AM To: P Sicurello Cc: HistoNet Subject: Re: [Histonet] Delay in embedding? We have done this - only on an emergency basis. Sent from my iPhone > On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet wrote: > > ?Good Morning Listers, > > > > How many out there will process tissue and then leave the cassettes at > room temperature and embed it at a later time (hours or the next day)? > > > > Please send me your opinions about doing this. I think it?s a bad > idea, others I speak with disagree. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 9300 Campus Point Drive > > La Jolla, CA 92037 > (P): 858-249-5610 > > > > *Confidentiality Notice*: The information transmitted in this e-mail > is intended only for the person or entity to which it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action > in reliance upon this information by persons or entities other than > the intended recipient is prohibited. If you received this e-mail in > error, please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From jsaundersht at yahoo.com Wed Dec 18 01:07:35 2019 From: jsaundersht at yahoo.com (Jennifer Saunders) Date: Wed, 18 Dec 2019 07:07:35 +0000 (UTC) Subject: [Histonet] Delay in embedding? In-Reply-To: References: <693B4D62-DF63-486C-9017-D24B9F190136@icloud.com> Message-ID: <920732173.736881.1576652855323@mail.yahoo.com> I don?t see a problem with it. We all melt and re-embed tissue for all kinds of reasons every day. I think it is much better for the tissue than keeping it warm for an extended period of time.?Jen Saunders? Sent from Yahoo Mail for iPhone On Tuesday, December 17, 2019, 2:28 PM, Tony Henwood (SCHN) via Histonet wrote: Hi Paula, We routinely do this, especially for our fetal autopsy blocks. We are then able to process and let them set at room temp until we are able to embed and cut. Some cases are more urgent than others so these can be expedited a lot easier since they will only need embedding, sectioning and staining. We also get best usage of our limited processing capabilities. It is more efficient for us. Morphology, staining and immunohistochemistry is not affected. It is better to do this rather than leave them at 64oC plus for extended times prior to embedding since many antigens will be adversely affected. Take home point - do not overcook your tissues. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Theresa Dalton via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 18 December 2019 7:34 AM To: P Sicurello Cc: HistoNet Subject: Re: [Histonet] Delay in embedding? We have done this - only on an emergency basis. Sent from my iPhone > On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet wrote: > > ?Good Morning Listers, > > > > How many out there will process tissue and then leave the cassettes at > room temperature and embed it at a later time (hours or the next day)? > > > > Please send me your opinions about doing this.? I think it?s a bad > idea, others I speak with disagree. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 9300 Campus Point Drive > > La Jolla, CA 92037 > (P): 858-249-5610 > > > > *Confidentiality Notice*: The information transmitted in this e-mail > is intended only for the person or entity to which it is addressed and > may contain confidential and/or privileged material.? Any review, > retransmission, dissemination or other use of or taking of any action > in reliance upon this information by persons or entities other than > the intended recipient is prohibited.? If you received this e-mail in > error, please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garreyf at gmail.com Wed Dec 18 04:09:23 2019 From: garreyf at gmail.com (Garrey Faller) Date: Wed, 18 Dec 2019 05:09:23 -0500 Subject: [Histonet] Delay in embedding? In-Reply-To: <920732173.736881.1576652855323@mail.yahoo.com> References: <920732173.736881.1576652855323@mail.yahoo.com> Message-ID: <31C3BB02-24D4-4C02-ABA5-C744B4FE74E9@gmail.com> I?ve done it a handful of times when my only Histotech called in sick. No effect on quality. Garrey Sent from my iPhone > On Dec 18, 2019, at 2:24 AM, Jennifer Saunders via Histonet wrote: > > ?I don?t see a problem with it. We all melt and re-embed tissue for all kinds of reasons every day. I think it is much better for the tissue than keeping it warm for an extended period of time. Jen Saunders > > > Sent from Yahoo Mail for iPhone > > > On Tuesday, December 17, 2019, 2:28 PM, Tony Henwood (SCHN) via Histonet wrote: > > Hi Paula, > > We routinely do this, especially for our fetal autopsy blocks. > We are then able to process and let them set at room temp until we are able to embed and cut. > Some cases are more urgent than others so these can be expedited a lot easier since they will only need embedding, sectioning and staining. We also get best usage of our limited processing capabilities. > It is more efficient for us. > > Morphology, staining and immunohistochemistry is not affected. > > It is better to do this rather than leave them at 64oC plus for extended times prior to embedding since many antigens will be adversely affected. > > Take home point - do not overcook your tissues. > > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children?s Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: Theresa Dalton via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, 18 December 2019 7:34 AM > To: P Sicurello > Cc: HistoNet > Subject: Re: [Histonet] Delay in embedding? > > We have done this - only on an emergency basis. > > Sent from my iPhone > >> On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet wrote: >> >> ?Good Morning Listers, >> >> >> >> How many out there will process tissue and then leave the cassettes at >> room temperature and embed it at a later time (hours or the next day)? >> >> >> >> Please send me your opinions about doing this. I think it?s a bad >> idea, others I speak with disagree. >> >> Sincerely, >> >> Paula Sicurello, HTL (ASCP)CM >> >> Histotechnology Specialist >> >> UC San Diego Health >> >> 9300 Campus Point Drive >> >> La Jolla, CA 92037 >> (P): 858-249-5610 >> >> >> >> *Confidentiality Notice*: The information transmitted in this e-mail >> is intended only for the person or entity to which it is addressed and >> may contain confidential and/or privileged material. Any review, >> retransmission, dissemination or other use of or taking of any action >> in reliance upon this information by persons or entities other than >> the intended recipient is prohibited. If you received this e-mail in >> error, please contact the sender and delete the material from any computer. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed Dec 18 10:32:13 2019 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 18 Dec 2019 11:32:13 -0500 Subject: [Histonet] Happy Holidays Histonetters! Here are the 12 days of Christmas for histotechs by Ashley Troutman. Enjoy! Message-ID: <0a5001d5b5c0$b402ac40$1c0804c0$@earthlink.net> Hi Histonetters, Happy Holidays! Here is a gem posted on the histonet in 12/2013 The 12 days of Christmas for Histotechs ! written by Ashley Troutman ?Hello all, I hope the holidays are treating everyone well. (Especially in light of us having the most hazardous job in the country...) Here's some fun for the season: The Twelve Days of Histo TWELVE Medical Directors ELEVEN logs-a-printing TEN residents reading NINE frozen sections EIGHT administrators SEVEN stainers beeping SIX techs complaining FIVE I...H...Cs.... :) FOUR calling docs THREE recuts TWO special stains And a block with an H and EEEEE... Please feel free to sing along. Merry Christmas in Histoland!? Ashley Troutman BS, MBA, HT(ASCP) QIHC Histonetters, I have some REALLY Exciting opportunities, Here?s a quick list: Leadership: MA - Field Applications Specialist ? IHC- Boston IL - Field Applications Specialist ? IHC ? Chicago MO - Histology Lab Manager ? St. Louis TN - Histology Supervisor - Nashville Histotechnicians/Histotechnologists North Carolina South Carolina Wisconsin Florida Kansas New York California I have new opportunities coming in all of the time. Let me know if anything looks interesting on my list or if there is a specific area you would like for me to be on the lookout for. We can start chatting now about opportunities after the new year!! The early bird gets the worm!! I also can help if you are short staffed! I can be reached asap on my cell/text at 407-353-5070 or email me at relia1 at earthlink.net Happy Holidays to you and yours!!!! Happy Holidays !!!! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From m.jamison at elitechgroup.com Wed Dec 18 11:51:40 2019 From: m.jamison at elitechgroup.com (Michelle Jamison) Date: Wed, 18 Dec 2019 10:51:40 -0700 Subject: [Histonet] plural fluid prep Message-ID: Hi All, I work in a research lab where we are using plural fluid to proof a method. Recently we have received some, and are unsure how long it will be ok in the fridge, or if we should add some preservation fluid to it. Can anyone advise please. Thank you in advance, and Happy Holidays to all!! -- Best of all things to you, Michelle Jamison // Tel : +1.435.752.6011 Ext. 435.227.1474 m.jamison at elitechgroup.com ? www.elitechgroup.com ------------------------------------------------------------------------ Logo From john.garratt at ciqc.ca Wed Dec 18 13:38:48 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Wed, 18 Dec 2019 19:38:48 +0000 Subject: [Histonet] plural fluid prep In-Reply-To: References: Message-ID: The cells have been living quite nicely in a natural culture medium at 37c so putting the pleural fluid at 4c will preserve the cells for a number of days. I do not think I would want to go past 3 to 4 days, but that is just the limit of my experience revisiting old ascitic fluid specimens. You could use alcohol to preserve the specimen longer but not knowing what your experiment is I am loath to offer an opinion on that. John Sent from ProtonMail Mobile On Wed, Dec 18, 2019 at 9:51 AM, Michelle Jamison via Histonet wrote: > Hi All, > I work in a research lab where we are using plural fluid to proof a > method. Recently we have received some, and are unsure how long it will > be ok in the fridge, or if we should add some preservation fluid to it. > Can anyone advise please. > Thank you in advance, and Happy Holidays to all!! > -- > > Best of all things to you, > > Michelle Jamison > > // > > Tel : +1.435.752.6011 Ext. 435.227.1474 > > m.jamison at elitechgroup.com ? www.elitechgroup.com > > > ------------------------------------------------------------------------ > > Logo > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Wed Dec 18 14:03:16 2019 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Wed, 18 Dec 2019 20:03:16 +0000 Subject: [Histonet] Delay in embedding? Message-ID: I agree with replies/statements 3, 4, 5 Anyone who states differently have not experienced this contingency. Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From Pez at uwyo.edu Wed Dec 18 14:19:45 2019 From: Pez at uwyo.edu (Rebecca E. Ashley) Date: Wed, 18 Dec 2019 20:19:45 +0000 Subject: [Histonet] Large Format Cover Glass Message-ID: Does anyone know of a good source for cover glass for the large format slides? 48x60mm specifically. We do a lot of eyes from large animals and I'm having a hard time finding coverslips big enough for a horse eye! Thanks, Rebecca Rebecca Ashley, HTL (ASCP)CM Laboratory Technician III Wyoming State Veterinary Laboratory 1174 Snowy Range Rd. Laramie, WY 82070 307-766-9946 From sandra.cheasty at wisc.edu Wed Dec 18 17:44:47 2019 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Wed, 18 Dec 2019 23:44:47 +0000 Subject: [Histonet] Deep Water Bath with Lighting Message-ID: Ho-ho-ho all, I'm looking for a deep water bath, (3" minimum), that has an interior light source. Most of them seem to be only 2" tall, or have a slanted front control panel that none of us in the lab want. Does anyone know of a brand of flotation water baths that are 3" deep and have interior lighting? Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From bcooper at chla.usc.edu Wed Dec 18 20:09:40 2019 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Thu, 19 Dec 2019 02:09:40 +0000 Subject: [Histonet] Deep Water Bath with Lighting In-Reply-To: References: Message-ID: I really like the TFB 35 flotation baths from Medite. They make a shallow and a deeper dish version; you'll want to go with the deeper dish. I'm not in front of one right now, but I suspect they're about 3" give or take. Thanks, Brian Cooper Histology Supervisor Children's Hospital Los Angeles Sent from my cell phone, so please excuse typos On Dec 18, 2019 3:45 PM, Sandra Cheasty via Histonet wrote: Ho-ho-ho all, I'm looking for a deep water bath, (3" minimum), that has an interior light source. Most of them seem to be only 2" tall, or have a slanted front control panel that none of us in the lab want. Does anyone know of a brand of flotation water baths that are 3" deep and have interior lighting? Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From jkempf80 at uga.edu Thu Dec 19 12:05:40 2019 From: jkempf80 at uga.edu (Jennifer Kempf) Date: Thu, 19 Dec 2019 18:05:40 +0000 Subject: [Histonet] Large Format Cover Glass In-Reply-To: References: Message-ID: We do a lot of large eyes and tissue sections as well. We use the HistoBond slides from Ted Pella. #260240 for the 2x3 slides, and #260378 for the corresponding coverslips. Hope this helps :-) Thanks, ~Jen~ Jennifer M Kempf Histology Laboratory Manager Department of Pathology College of Veterinary Medicine University of Georgia 501 D.W. Brooks Drive Athens, Georgia 30602 Office: 706-542-2218 Lab: 706-542-5835 jkempf80 at uga.edu -----Original Message----- From: Rebecca E. Ashley Sent: Wednesday, December 18, 2019 3:20 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Large Format Cover Glass Does anyone know of a good source for cover glass for the large format slides? 48x60mm specifically. We do a lot of eyes from large animals and I'm having a hard time finding coverslips big enough for a horse eye! Thanks, Rebecca Rebecca Ashley, HTL (ASCP)CM Laboratory Technician III Wyoming State Veterinary Laboratory 1174 Snowy Range Rd. Laramie, WY 82070 307-766-9946 From tbraud at holyredeemer.com Thu Dec 19 12:11:27 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 19 Dec 2019 18:11:27 +0000 Subject: [Histonet] Delay in embedding Message-ID: <48E053DDF6CE074DB6A7414BA05403F801C1B6C27B@HRHEX02-HOS.holyredeemer.local> We do it all the time. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Wednesday, December 18, 2019 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 193, Issue 12 CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links or opening attachments. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Delay in embedding? (P Sicurello) 2. Re: Delay in embedding? (Theresa Dalton) 3. Re: Delay in embedding? (Tony Henwood (SCHN)) 4. Re: Delay in embedding? (Jennifer Saunders) 5. Re: Delay in embedding? (Garrey Faller) 6. Happy Holidays Histonetters! Here are the 12 days of Christmas for histotechs by Ashley Troutman. Enjoy! (Pam Barker) 7. plural fluid prep (Michelle Jamison) ---------------------------------------------------------------------- Message: 1 Date: Tue, 17 Dec 2019 11:30:58 -0800 From: P Sicurello To: HistoNet Subject: [Histonet] Delay in embedding? Message-ID: Content-Type: text/plain; charset="UTF-8" Good Morning Listers, How many out there will process tissue and then leave the cassettes at room temperature and embed it at a later time (hours or the next day)? Please send me your opinions about doing this. I think it?s a bad idea, others I speak with disagree. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ------------------------------ Message: 2 Date: Tue, 17 Dec 2019 15:33:43 -0500 From: Theresa Dalton To: P Sicurello Cc: HistoNet Subject: Re: [Histonet] Delay in embedding? Message-ID: <693B4D62-DF63-486C-9017-D24B9F190136 at icloud.com> Content-Type: text/plain; charset=utf-8 We have done this - only on an emergency basis. Sent from my iPhone > On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet wrote: > > ?Good Morning Listers, > > > > How many out there will process tissue and then leave the cassettes at room > temperature and embed it at a later time (hours or the next day)? > > > > Please send me your opinions about doing this. I think it?s a bad idea, > others I speak with disagree. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 9300 Campus Point Drive > > La Jolla, CA 92037 > (P): 858-249-5610 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 17 Dec 2019 22:28:13 +0000 From: "Tony Henwood (SCHN)" To: Theresa Dalton , P Sicurello Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] Delay in embedding? Message-ID: Content-Type: text/plain; charset="utf-8" Hi Paula, We routinely do this, especially for our fetal autopsy blocks. We are then able to process and let them set at room temp until we are able to embed and cut. Some cases are more urgent than others so these can be expedited a lot easier since they will only need embedding, sectioning and staining. We also get best usage of our limited processing capabilities. It is more efficient for us. Morphology, staining and immunohistochemistry is not affected. It is better to do this rather than leave them at 64oC plus for extended times prior to embedding since many antigens will be adversely affected. Take home point - do not overcook your tissues. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Theresa Dalton via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 18 December 2019 7:34 AM To: P Sicurello Cc: HistoNet Subject: Re: [Histonet] Delay in embedding? We have done this - only on an emergency basis. Sent from my iPhone > On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet wrote: > > ?Good Morning Listers, > > > > How many out there will process tissue and then leave the cassettes at > room temperature and embed it at a later time (hours or the next day)? > > > > Please send me your opinions about doing this. I think it?s a bad > idea, others I speak with disagree. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 9300 Campus Point Drive > > La Jolla, CA 92037 > (P): 858-249-5610 > > > > *Confidentiality Notice*: The information transmitted in this e-mail > is intended only for the person or entity to which it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action > in reliance upon this information by persons or entities other than > the intended recipient is prohibited. If you received this e-mail in > error, please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ------------------------------ Message: 4 Date: Wed, 18 Dec 2019 07:07:35 +0000 (UTC) From: Jennifer Saunders To: "Tony Henwood (SCHN)" , Theresa Dalton , P Sicurello Cc: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] Delay in embedding? Message-ID: <920732173.736881.1576652855323 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 I don?t see a problem with it. We all melt and re-embed tissue for all kinds of reasons every day. I think it is much better for the tissue than keeping it warm for an extended period of time.?Jen Saunders? Sent from Yahoo Mail for iPhone On Tuesday, December 17, 2019, 2:28 PM, Tony Henwood (SCHN) via Histonet wrote: Hi Paula, We routinely do this, especially for our fetal autopsy blocks. We are then able to process and let them set at room temp until we are able to embed and cut. Some cases are more urgent than others so these can be expedited a lot easier since they will only need embedding, sectioning and staining. We also get best usage of our limited processing capabilities. It is more efficient for us. Morphology, staining and immunohistochemistry is not affected. It is better to do this rather than leave them at 64oC plus for extended times prior to embedding since many antigens will be adversely affected. Take home point - do not overcook your tissues. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Theresa Dalton via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 18 December 2019 7:34 AM To: P Sicurello Cc: HistoNet Subject: Re: [Histonet] Delay in embedding? We have done this - only on an emergency basis. Sent from my iPhone > On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet wrote: > > ?Good Morning Listers, > > > > How many out there will process tissue and then leave the cassettes at > room temperature and embed it at a later time (hours or the next day)? > > > > Please send me your opinions about doing this.? I think it?s a bad > idea, others I speak with disagree. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 9300 Campus Point Drive > > La Jolla, CA 92037 > (P): 858-249-5610 > > > > *Confidentiality Notice*: The information transmitted in this e-mail > is intended only for the person or entity to which it is addressed and > may contain confidential and/or privileged material.? Any review, > retransmission, dissemination or other use of or taking of any action > in reliance upon this information by persons or entities other than > the intended recipient is prohibited.? If you received this e-mail in > error, please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 18 Dec 2019 05:09:23 -0500 From: Garrey Faller To: Jennifer Saunders Cc: "Tony Henwood (SCHN)" , Theresa Dalton , P Sicurello , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Delay in embedding? Message-ID: <31C3BB02-24D4-4C02-ABA5-C744B4FE74E9 at gmail.com> Content-Type: text/plain; charset=utf-8 I?ve done it a handful of times when my only Histotech called in sick. No effect on quality. Garrey Sent from my iPhone > On Dec 18, 2019, at 2:24 AM, Jennifer Saunders via Histonet wrote: > > ?I don?t see a problem with it. We all melt and re-embed tissue for all kinds of reasons every day. I think it is much better for the tissue than keeping it warm for an extended period of time. Jen Saunders > > > Sent from Yahoo Mail for iPhone > > > On Tuesday, December 17, 2019, 2:28 PM, Tony Henwood (SCHN) via Histonet wrote: > > Hi Paula, > > We routinely do this, especially for our fetal autopsy blocks. > We are then able to process and let them set at room temp until we are able to embed and cut. > Some cases are more urgent than others so these can be expedited a lot easier since they will only need embedding, sectioning and staining. We also get best usage of our limited processing capabilities. > It is more efficient for us. > > Morphology, staining and immunohistochemistry is not affected. > > It is better to do this rather than leave them at 64oC plus for extended times prior to embedding since many antigens will be adversely affected. > > Take home point - do not overcook your tissues. > > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children?s Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: Theresa Dalton via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Wednesday, 18 December 2019 7:34 AM > To: P Sicurello > Cc: HistoNet > Subject: Re: [Histonet] Delay in embedding? > > We have done this - only on an emergency basis. > > Sent from my iPhone > >> On Dec 17, 2019, at 2:48 PM, P Sicurello via Histonet wrote: >> >> ?Good Morning Listers, >> >> >> >> How many out there will process tissue and then leave the cassettes at >> room temperature and embed it at a later time (hours or the next day)? >> >> >> >> Please send me your opinions about doing this. I think it?s a bad >> idea, others I speak with disagree. >> >> Sincerely, >> >> Paula Sicurello, HTL (ASCP)CM >> >> Histotechnology Specialist >> >> UC San Diego Health >> >> 9300 Campus Point Drive >> >> La Jolla, CA 92037 >> (P): 858-249-5610 >> >> >> >> *Confidentiality Notice*: The information transmitted in this e-mail >> is intended only for the person or entity to which it is addressed and >> may contain confidential and/or privileged material. Any review, >> retransmission, dissemination or other use of or taking of any action >> in reliance upon this information by persons or entities other than >> the intended recipient is prohibited. If you received this e-mail in >> error, please contact the sender and delete the material from any computer. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Wed, 18 Dec 2019 11:32:13 -0500 From: "Pam Barker" To: "Histopeeps Histonet" Subject: [Histonet] Happy Holidays Histonetters! Here are the 12 days of Christmas for histotechs by Ashley Troutman. Enjoy! Message-ID: <0a5001d5b5c0$b402ac40$1c0804c0$@earthlink.net> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters, Happy Holidays! Here is a gem posted on the histonet in 12/2013 The 12 days of Christmas for Histotechs ! written by Ashley Troutman ?Hello all, I hope the holidays are treating everyone well. (Especially in light of us having the most hazardous job in the country...) Here's some fun for the season: The Twelve Days of Histo TWELVE Medical Directors ELEVEN logs-a-printing TEN residents reading NINE frozen sections EIGHT administrators SEVEN stainers beeping SIX techs complaining FIVE I...H...Cs.... :) FOUR calling docs THREE recuts TWO special stains And a block with an H and EEEEE... Please feel free to sing along. Merry Christmas in Histoland!? Ashley Troutman BS, MBA, HT(ASCP) QIHC Histonetters, I have some REALLY Exciting opportunities, Here?s a quick list: Leadership: MA - Field Applications Specialist ? IHC- Boston IL - Field Applications Specialist ? IHC ? Chicago MO - Histology Lab Manager ? St. Louis TN - Histology Supervisor - Nashville Histotechnicians/Histotechnologists North Carolina South Carolina Wisconsin Florida Kansas New York California I have new opportunities coming in all of the time. Let me know if anything looks interesting on my list or if there is a specific area you would like for me to be on the lookout for. We can start chatting now about opportunities after the new year!! The early bird gets the worm!! I also can help if you are short staffed! I can be reached asap on my cell/text at 407-353-5070 or email me at relia1 at earthlink.net Happy Holidays to you and yours!!!! Happy Holidays !!!! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Message: 7 Date: Wed, 18 Dec 2019 10:51:40 -0700 From: Michelle Jamison To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] plural fluid prep Message-ID: Content-Type: text/plain; charset=utf-8; format=flowed Hi All, I work in a research lab where we are using plural fluid to proof a method. Recently we have received some, and are unsure how long it will be ok in the fridge, or if we should add some preservation fluid to it. Can anyone advise please. Thank you in advance, and Happy Holidays to all!! -- Best of all things to you, Michelle Jamison // Tel : +1.435.752.6011 Ext. 435.227.1474 m.jamison at elitechgroup.com ? www.elitechgroup.com ------------------------------------------------------------------------ Logo ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 193, Issue 12 ***************************************** From areed46254 at yahoo.com Thu Dec 19 13:02:42 2019 From: areed46254 at yahoo.com (amy reed) Date: Thu, 19 Dec 2019 19:02:42 +0000 (UTC) Subject: [Histonet] Large cover glass References: <1704639597.1515483.1576782162740.ref@mail.yahoo.com> Message-ID: <1704639597.1515483.1576782162740@mail.yahoo.com> I have had great luck with https://brainresearchlab.com/? they have a large assortment slides and glass From amosbrooks at gmail.com Sun Dec 22 11:30:30 2019 From: amosbrooks at gmail.com (Amos Brooks) Date: Sun, 22 Dec 2019 12:30:30 -0500 Subject: [Histonet] plural fluid prep In-Reply-To: References: Message-ID: > > > Hi Michelle, Pleural fluid lasts a fairly long time if you don't centrifuge it and leave it in suspension. Of course it will last even longer if properly fixed. I have returned to a stored (unfixed) pleural fluid for retrospective testing up to a week later. The longer you let it sit though, the more complications can occur like bacterial growth and further call division or death depending on what's going on in the fluid. Amos Brooks > > Message: 7 > Date: Wed, 18 Dec 2019 10:51:40 -0700 > From: Michelle Jamison > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] plural fluid prep > Message-ID: > Content-Type: text/plain; charset=utf-8; format=flowed > > Hi All, > I work in a research lab where we are using plural fluid to proof a > method. Recently we have received some, and are unsure how long it will > be ok in the fridge, or if we should add some preservation fluid to it. > Can anyone advise please. > From hinderaker.s at gmail.com Mon Dec 23 01:23:31 2019 From: hinderaker.s at gmail.com (stephanie h) Date: Mon, 23 Dec 2019 00:23:31 -0700 Subject: [Histonet] plural fluid prep In-Reply-To: References: Message-ID: <7C5D95BB-A4A6-4120-A5DA-A0B02C553B5C@gmail.com> Can you spin it down and make into blocks? Does it have to be fresh PF? > On Dec 22, 2019, at 10:45 AM, Amos Brooks via Histonet wrote: > > ? >> >> >> >> Hi Michelle, > Pleural fluid lasts a fairly long time if you don't centrifuge it and > leave it in suspension. Of course it will last even longer if properly > fixed. I have returned to a stored (unfixed) pleural fluid for > retrospective testing up to a week later. > The longer you let it sit though, the more complications can occur > like bacterial growth and further call division or death depending on > what's going on in the fluid. > > Amos Brooks > >> >> Message: 7 >> Date: Wed, 18 Dec 2019 10:51:40 -0700 >> From: Michelle Jamison >> To: "histonet at lists.utsouthwestern.edu" >> >> Subject: [Histonet] plural fluid prep >> Message-ID: >> Content-Type: text/plain; charset=utf-8; format=flowed >> >> Hi All, >> I work in a research lab where we are using plural fluid to proof a >> method. Recently we have received some, and are unsure how long it will >> be ok in the fridge, or if we should add some preservation fluid to it. >> Can anyone advise please. >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bethcoxx at gmail.com Mon Dec 23 12:55:41 2019 From: bethcoxx at gmail.com (Beth Cox) Date: Mon, 23 Dec 2019 13:55:41 -0500 Subject: [Histonet] plural fluid prep Message-ID: <71ece317-6330-24f7-e25e-48ae25dfc8bb@gmail.com> Hi Michelle, When I was in my Cytology training, we did an experiment to answer just that question.? We started with a 2 liter jug of plural fluid with nothing added, and we stored in in the fridge.? At first we tested it every day to see if there was any degeneration, then we got tired of that and tested twice a week, then once a week. After 4 months it was still just fine, and we threw it away because we were tired of testing it (and it was the end of the semester!).?? It seems that the cells are happy in their natural habitat and refrigeration slowed/prevented any bacterial growth. So? my advice is:? don't add anything to it, keep it in the fridge, and it will last many months!! Beth Cox, SCT/HTL(ASCP)QIHC From: Michelle Jamison To:"histonet at lists.utsouthwestern.edu" Subject: [Histonet] plural fluid prep Message-ID: Content-Type: text/plain; charset=utf-8; format=flowed Hi All, I work in a research lab where we are using plural fluid to proof a method. Recently we have received some, and are unsure how long it will be ok in the fridge, or if we should add some preservation fluid to it. Can anyone advise please. From doolee at shands.ufl.edu Fri Dec 27 15:49:50 2019 From: doolee at shands.ufl.edu (Dooley, Elaine) Date: Fri, 27 Dec 2019 21:49:50 +0000 Subject: [Histonet] Histology job! Message-ID: <253979c5de2f4835a294b71a7e9e2ca6@shands.ufl.edu> Hi histonetters, We are looking for histologists who love to learn and work in a busy lab that does many different tests with all types of tissues. We do IHC, special stains, muscle biopsy special stains, IF on skins and kidney biopsies and much more. Please look us up on our website. UF Health Shands Hospital Jobs. Gainesville Florida is the home of the University of Florida, so there is much to do and learn in this community. Gainesville is not as big as Orlando or Tampa, so we do not have bad traffic and we are close to many fresh water springs, rivers and lakes. Kayaking, fishing and hiking are great in this area. Also we are not far from either coast if you want to go to the beach for the day or weekend. Job Title Histologist- Surgical Pathology- Gainesville Florida UF Health Shands Hospital Elaine Dooley Lead Tech IHC UF Health Shands Hospital From kaarrington at anthc.org Mon Dec 30 09:46:03 2019 From: kaarrington at anthc.org (Arrington, Karla A) Date: Mon, 30 Dec 2019 15:46:03 +0000 Subject: [Histonet] Histology Position in AK Message-ID: <49a6820f7b4947c9a30624788fd922b6@anthc.org> In beautiful Alaska, our hospital caters to the Alaskan Native population with their entire medical needs being met. Currently we have a Histology Technician position available in our pathology department. This position is M-F, no weekends and a rotating on-call schedule. This position comes with excellent benefits and a team work environment. This also offers relocation assistance for the correct candidate. Must be ASCP certified or eligible. For immediate consideration, email or FAX a current resume to kaarrington at anthc.org and it will be forwarded to our hiring manager. Also visit our website at anthc.org and select Careers to apply online. If you have questions regarding this position or how to submit an online application, please call ANTHC Human Resources at (907) 729-1301 or email careers at anthc.org. Karla Arrington, HT(ASCP) HIT(AHIMA) Pathology Supervisor Alaska Native Medical Center 4315 Diplomacy Drive Anchorage, AK 99508 Tele: 907-729-1810 Fax: 907-729-1226 From susanhay59 at gmail.com Tue Dec 31 09:22:44 2019 From: susanhay59 at gmail.com (S hay) Date: Tue, 31 Dec 2019 09:22:44 -0600 Subject: [Histonet] Pap stains Message-ID: 1. Does everyone filter their pap stains daily? 2. Are you chaining all other reagents daily? Thanks in advance. From Elizabeth.Haas at slh.wisc.edu Tue Dec 31 10:46:53 2019 From: Elizabeth.Haas at slh.wisc.edu (Haas, Elizabeth) Date: Tue, 31 Dec 2019 16:46:53 +0000 Subject: [Histonet] Pap stains In-Reply-To: References: Message-ID: <5135B39B-B5D1-42A8-ADD0-9224273D34FC@slh.wisc.edu> I believe filtering stains daily is a CAP requirement Sent from my iPhone > On Dec 31, 2019, at 9:23 AM, S hay via Histonet wrote: > > ?1. Does everyone filter their pap stains daily? > 2. Are you chaining all other reagents daily? > > Thanks in advance. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ LEGAL DISCLAIMER: This message and all attachments may be confidential or protected by privilege. 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