From relia1 at earthlink.net Thu Aug 1 08:59:23 2019 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 1 Aug 2019 09:59:23 -0400 Subject: [Histonet] Hospitalist Histologist - Salem/Roanoke, VA Message-ID: <00be01d54871$52fec3f0$f8fc4bd0$@earthlink.net> Hi Histopeeps! I hope you are having a great day. I am currently working with a client who is in need of an ASCP certified or eligible histotech for a full time day shift position in a hospital in Salem, VA. My client is offering the opportunity to work in all areas of histology so you won?t be stuck just cutting and embedding. If you can perform IHC great!! If not you have the opportunity to learn IHC. My client is offering a competitive pay rate, great benefits and relocation assistance. For more information please contact me You can reach me asap on my cell at 407-353-5070 or toll free at the office at 866-607-3542 or via email at relia1 at earthlink.net Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From robrankin at rankinbiomed.com Fri Aug 2 09:11:59 2019 From: robrankin at rankinbiomed.com (Rob Rankin) Date: Fri, 2 Aug 2019 10:11:59 -0400 Subject: [Histonet] Histonet Digest, Vol 188, Issue 22 In-Reply-To: References: Message-ID: Subject: Pathologist needed for charity work A pathologist is greatly needed to examine slides for a private lab in Ghana. If you, or someone you know, is empathetic to helping people in underdeveloped countries, please reply for more information. Histology labs in Ghana are far and few between, but more and more are popping up to deliver much needed cancer diagnosis to the Ghanaians. > > Respectfully, *Rob Rankin, MSM, SM(ASCP)* Founder & CEO O: 248.625.4104 ext 0012 www.rankinbiomed.com 14515 Mackey Rd. Holly, MI 48442 From tbraud at holyredeemer.com Fri Aug 2 09:30:26 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 2 Aug 2019 14:30:26 +0000 Subject: [Histonet] Ventana UltraView Contamination Message-ID: <48E053DDF6CE074DB6A7414BA05403F801A226E178@HRHEX03-HOS.holyredeemer.local> Heads up, Histo peeps! We have 2 ultraView kits, both from the same lot #F03020 that have extra plugs? Black things? Floating around in the Multimer reagent. Please check your kits carefully. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From PKRichar at gundersenhealth.org Fri Aug 2 12:43:06 2019 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Fri, 2 Aug 2019 17:43:06 +0000 Subject: [Histonet] Safety Glasses Message-ID: Would anyone be willing to share your requirements for wearing safety glasses in your laboratory? Cordially, Pam ~ National Histology Professionals Day 3/10/19 Pathologist Assistant Day 4/14/2019 Medical Laboratory Professionals Week April 21-27, 2019 National Cytotechnology Day 5/13/2019 +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org From shive003 at umn.edu Mon Aug 5 11:41:48 2019 From: shive003 at umn.edu (Jan Shivers) Date: Mon, 5 Aug 2019 11:41:48 -0500 Subject: [Histonet] Influenza A IHC Message-ID: If anyone is still doing Influenza A IHC testing, could you share your vendor source and catalog number with me? I'm looking to find a better source than what I'm using right now. I don't do it very often (since we have a PCR test) but once in awhile it's requested, and my old antibody just doesn't seem to detect all that it should. Thanks in advance, Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003 at umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* From acanabal at ciencias.unam.mx Mon Aug 5 11:59:10 2019 From: acanabal at ciencias.unam.mx (=?UTF-8?Q?Alonso_Mart=C3=ADnez_Canabal?=) Date: Mon, 5 Aug 2019 11:59:10 -0500 Subject: [Histonet] DAB enhaced with Nickel Sulfate Ammonium Message-ID: Good Afternoon. It is great to be in this list again. My first e-mail is to ask your advise about a little problem that we have in the lab. We are doing immunohistochemistry in brain tissue using DAB enhanced with nickel ammonium sulfate. The result is great, with a very clean signal in 50um thickness brain sections. Our problem is that later we do some counterstaining with methyl green. It is purified with chorophorm and in pH 4.2 in Acetate buffer. We clearly see the green nuclei and the immunoreactive cells, but apparently, after the counterstaining, the dark-purple-blackish precipitate is gone and we can only see regular brown DAB. What do you think? I wonder if it is the pH of the Methyl green, but I am kind of lost here. Any advise? Thank you very much Dr. Alonso -- Dr. Alonso Mart?nez Canabal PhD Profesor Asociado "C" Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833 From rjbuesa at yahoo.com Mon Aug 5 12:11:41 2019 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 5 Aug 2019 17:11:41 +0000 (UTC) Subject: [Histonet] DAB enhaced with Nickel Sulfate Ammonium In-Reply-To: References: Message-ID: <342468152.1272798.1565025101013@mail.yahoo.com> Better try another counterstaining. Will be easier in the long run.Ren? On Monday, August 5, 2019, 01:08:38 PM EDT, Alonso Mart?nez Canabal via Histonet wrote: ? Good Afternoon. ? ? ? ? It is great to be in this list again. My first e-mail is to ask your advise about a little problem that we have in the lab. ? ? ? We are doing immunohistochemistry in brain tissue using DAB enhanced with nickel ammonium sulfate. The result is great, with a very clean signal in 50um thickness brain sections. Our problem is that later we do some counterstaining with methyl green. It is purified with chorophorm and in pH 4.2 in Acetate buffer. We clearly see the green nuclei and the immunoreactive cells, but apparently, after the counterstaining, the dark-purple-blackish precipitate is gone and we can only see regular brown DAB. ? ? What do you think? ? I wonder if it is the pH of the Methyl green, but I am kind of lost here. Any advise? ? ? Thank you very much ? ? ? ? ? Dr. Alonso -- Dr. Alonso Mart?nez Canabal PhD Profesor Asociado "C" Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katherine at ka-recruiting.com Mon Aug 5 14:00:00 2019 From: katherine at ka-recruiting.com (Katherine Marano) Date: Mon, 5 Aug 2019 15:00:00 -0400 Subject: [Histonet] Histotech opening in Virginia Message-ID: Hi Histonetters! Happy Monday - I hope you had a great weekend! I work at K.A. Recruiting specializing in the placement of healthcare professionals into permanent and full-time positions nationwide, and I am currently working with a lab in *Roanoke County, Virginia* that is looking to hire a Histotech for their M-F 4am-12:30pm shift. If you are interested, could you send me a resume and a good time to get in touch? They are looking to start the interview process asap so I would love to give you more information. I also have some other openings, if you or anyone you know may be interested let me know!! Central Georgia - Histotech x 2 openings (days 7:30a-4p and a morning 3a-1130a) Rockford, IL area- Histotech Chicago, IL area - Histotech x 2 openings (midnight ) anatomic pathology Northern Montana - Histotech Charlotte, NC area - Histotech North Dakota - Histology Lead (Monday-Friday day shift) White Plains, NY - Grossing Tech Nashville, TN area - Grosser (evening/ night shift) Again all of my positions are permanent and my clients offer great compensation and benefits, as well as relocation assistance and sign on bonuses! Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From relia1 at earthlink.net Tue Aug 6 11:06:45 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 6 Aug 2019 12:06:45 -0400 Subject: [Histonet] RELIA Histology Job Posting Bulletin Message-ID: <000001d54c70$f1df0270$d59d0750$@earthlink.net> Hello Histopeeps, I hope you are having a great day!! I have some new opportunities to share. HT/HTL FL ? Tallahassee Sign on Bonus!! AR ? Fayetteville ? Days!! VA ? Salem/Roanoke learn IHC! CA ? San Francisco Bay Area ? Days! CA ? San Diego ? Days CA- Chico - Days CA ? Berkeley ? Days Special procedures ? muscle biopsies WI ? Milwaukee ? Days!! GA ? Columbus ? Run your own lab All you have to do is contact me, if you want to chat right away call or text me at 407-353-5070 or you can reach me at relia1 at earthlink.net or call me toll free at 866-607-3542 my office is open M-F 7am-8pm EST or by appointment. Remember it never hurts to look!!! Thanks, Pam 877-60 RELIA (877-607-3542) Cell/Text (407)353-5070 I know there are a lot of recruiters out there right now contacting you and your friends. RELIA is the ONLY nationwide recruiting firm specializing in the permanent placement of histology professionals. Remember here at RELIA we work as your confidential advocate to help you make the move that is right for you when the time is right for you. For more detailed information first crack at new openings and a little fun subscribe to RELIA's Histology Careers Bulletin shoot me an email and I will sign you up! If you know of anyone else who might be interested in subscribing to RELIA?s Histology Careers Bulletin please feel free to pass this along to them remember if I place them now or at a later date you will earn a referral bonus!!. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From LRaff at uropartners.com Tue Aug 6 12:57:23 2019 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 6 Aug 2019 17:57:23 +0000 Subject: [Histonet] It's a blog, and a reminder to my friends in labs all around Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF124E41C6@COLOEXCH01.uropartners.local> http://www.chicagonow.com/downsize-maybe/2019/08/another-psa-for-p-s-a/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From bakevictoria at gmail.com Tue Aug 6 13:17:51 2019 From: bakevictoria at gmail.com (Victoria Baker) Date: Tue, 6 Aug 2019 14:17:51 -0400 Subject: [Histonet] CD27 In-Reply-To: References: Message-ID: On Mon, Jul 29, 2019, 9:45 PM Victoria Baker wrote: > Hi! > > Is anyone currently using CD27 on FFPE human tissue? > > I found one source and it is for lyophilized CD27. I'd like to find one > that is a concentrate. I have given up on a pre-dilute. I would like to > see if there is both a mono and a poly clonal ab available and is I'm use. > > Thank you in advance for your help. > > Vikki > > Thank > From Linda.Margraf at cookchildrens.org Wed Aug 7 07:51:42 2019 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Wed, 7 Aug 2019 12:51:42 +0000 Subject: [Histonet] Histotech job Gainesville FL Message-ID: <204388a0e488426890342eb6f8b83a83@MBX10.CCHCS.LDAP> Here is a message I am posting for Martha regarding a job at U of Florida. Her email is mct at ufl.edu if you are interested (or check their website). Begin forwarded message: From: "Campbell-Thompson, Martha" > Date: August 6, 2019 at 9:33:23 AM CDT To: "'histonet-owner at lists.utsouthwestern.edu'" > Subject: Request to post a position Histotech opening available at the University of Florida Molecular Pathology Core- please see position #511862. Full time- 8 am- 5:00 pm with no call or weekends. Closed all major holidays and generous benefits package including sick and vacation leave. Previous histology (research or clinical) experience required. Martha Campbell-Thompson, DVM PhD| Professor University of Florida | Pathology, Immunology, and Lab. Medicine College of Medicine | Diabetes Institute | JDRF nPOD | Director, Molecular Pathology Core College of Engineering |Biomedical Engineering PO Box 100275|1395 Center Drive, Gainesville, FL 32610|Phone: 352.273.6129|Email: mct at ufl.edu | From criley at dpspa.com Thu Aug 8 08:09:37 2019 From: criley at dpspa.com (Charles Riley) Date: Thu, 8 Aug 2019 09:09:37 -0400 Subject: [Histonet] Lab temperature Message-ID: Hello everyone, Where does everyone set their lab temperature ideally? I know in some places its difficult to keep the temp stable but what would everyone recommend as the best setting? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From tony.henwood at health.nsw.gov.au Thu Aug 8 23:51:33 2019 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 9 Aug 2019 04:51:33 +0000 Subject: [Histonet] Victoria blue for lung tissue In-Reply-To: <1564188227985.76571@health.nsw.gov.au> References: , <1564188227985.76571@health.nsw.gov.au> Message-ID: <35d21e37d5ba420ba4974a463ddd374f@SVDCMBX-MEX024.nswhealth.net> Whoops, The Ventana does not use Victoria blue, my mistake. They have a resorcin fuchsin solution (known as Hart's method) not Miller Victoria blue. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Tony Henwood (SCHN) Sent: Saturday, 27 July 2019 10:44 AM To: Bob Richmond Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Victoria blue for lung tissue Victoria Blue is the dye used in Miller's Stain for Elastic Tissue. It is also used in the Roche Ventana Benchmark Stainer to stain elastic Tissue: Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148-149 Karen Percival & Zaher Radi (2017) Comparison of five elastin histochemical stains to identify pulmonary small vasculature, Journal of Histotechnology, 40:3, 73-78 Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in Human Skin" Histologic 32(1): 12 Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 1996: 23: 37-42. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Bob Richmond via Histonet Sent: Saturday, July 27, 2019 4:10 AM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Victoria blue for lung tissue Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE lung tissue to exam thickness of artery. Could anybody recommend a good vendor of this reagent kit?<< You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and several others. I couldn't find anyone who offers a kit, only the dry dye. There are other dyes called Victoria Blue, reported to give the same results. You'd have to find a method for preparing it as an elastic stain, and I couldn't find such a method either with Google or in my old books. The requester needs to supply you with a method. I suspect the requester is reading an old article. There are stains for elastic tissue (which is I suppose what you want to "exam thickness of artery") that are a lot easier to get. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From abijag76 at yahoo.co.in Fri Aug 9 11:29:22 2019 From: abijag76 at yahoo.co.in (abi jag) Date: Fri, 9 Aug 2019 16:29:22 +0000 (UTC) Subject: [Histonet] Recent issues with picro sirius red staining (entire liver section become red, no yellow background) References: <613985784.3074148.1565368162399.ref@mail.yahoo.com> Message-ID: <613985784.3074148.1565368162399@mail.yahoo.com> Hello Histonetters,I am writing this to seek your help regarding a very recent problem that I am currently facing with Picro Sirius red staining of lab animal (mouse and rat) liver samples. I follow the procedure that was provided by John Kiernan in the histonet archives (please see below), which was working very well. Quite recently, the complete section become stained as red. Usually, collagen in the sections get stained as red with a yellow back ground. Please note that there was no change in the procedure/reagents etc, It will be of great help if you help me in troubleshooting this issue.With my best regards,Abijag Sirius red collagen procedure | | | | Sirius red collagen procedure | | | Solution A. Picro-sirius red Sirius red F3B (C.I. 35782): 0.5 g Saturated aqueous solution of picric acid: 500 ml Add a little solid picric acid to ensure saturation (This is important). (Keeps for at least 3 years and can be used many times.) Solution B. Acidified water Add 5 ml acetic acid (glacial) to 1 litre of water (tap or distilled). Procedure Fixation is not critical, The method is most frequently used on paraffin sections of objects fixed adequately (at least 24 hours but ideally 1 or 2 weeks) in a neutral buffered formaldehyde solution. 1. De-wax and hydrate paraffin sections. 2. (Optional, and not usually done) Stain nuclei with Weigert's haematoxylin (as for the van Gieson method, but more strongly, then wash the slides for 10 minutes in running tap water). 3. Stain in picro-sirius red (Solution A) for one hour. (This gives near-equilibrium staining, which does not increase with longer times. Shorter times should not be used, even if the colours look OK.) 4. Wash in two changes of acidified water (Solution B). 5. Physically remove most of the water from the slides by vigorous shaking or (for a few slides only) blotting with damp filter paper. 5. Dehydrate in three changes of 100% ethanol. 6. Clear in xylene and mount in a resinous medium. From robrankin at rankinbiomed.com Fri Aug 9 14:39:30 2019 From: robrankin at rankinbiomed.com (Rob Rankin) Date: Fri, 9 Aug 2019 15:39:30 -0400 Subject: [Histonet] PAS on the ST5020 Message-ID: Subject: PAS on the ST5020 Hello, Does anyone run PAS on an ST5020? If so, would you mind sharing the protocol that you use? I am asking on behalf of one of my customers that needs to program a PAS protocol on her ST5020. Thank you very much! Best, *Justin Rabidoux* www.rankinbiomed.com 14515 Mackey Rd. Holly, MI 48442 From jkiernan at uwo.ca Fri Aug 9 23:29:21 2019 From: jkiernan at uwo.ca (John Kiernan) Date: Sat, 10 Aug 2019 04:29:21 +0000 Subject: [Histonet] Recent issues with picro sirius red staining (entire liver section become red, no yellow background) In-Reply-To: <613985784.3074148.1565368162399@mail.yahoo.com> References: <613985784.3074148.1565368162399.ref@mail.yahoo.com>, <613985784.3074148.1565368162399@mail.yahoo.com> Message-ID: I've never seen the kind of staining you describe, abi jag, with "the complete section become stained as red" but I've never used the method on sections of liver. You should get red collagen and yellow hepatocytes with blue nuclei. The strongly acidic picrosirius stain, applied for an hour, always greatly weakens (differentiates) a prior nuclear stain with Weigert's or Lillie's iron-haematoxylin. It's also quite easy to lose some or all the yellow in the washing and dehydration of the stained sections. Most users of this method are interested only in collagen fibres and do not mind if the nuclear and cytoplasmic colours get lost. The iron-haematoxylin nuclear stain is often omitted. It is necessary to have the right dye. It must be sirius red F3B (= CI 35780 = Direct red 80). This is still used as a textile dye, with several suppliers and trade-names. See http://www.worlddyevariety.com/direct-dyes/direct-red-80.html#respond Direct Red 80 - worlddyevariety.com www.worlddyevariety.com List of Suppliers: Direct Red F3,Direct Fast Red BA,Direct Fast Red F3B. Tianjin Yadong Group . ACDI Red 8 BLN(Aakash Chemicals & Dyestuffs Inc)Alacodirect Red 2BL(Classic Dyestuffs Inc)AmbidirectRed 3BL( Thai Ambica Chemicals Co Ltd) Anadurm Red D-BA( Albion Colours Ltd) Arid Red 8 BLN ( Aashiana Dyestuffs Inc) Best Direct Supra Red F3B( Oriental Giant Dyes and Chemical Ind Corp) Other dyes with "sirius red" in their trivial names probably are not suitable if they are not the product recognized in the Colour Index as CI 35780, Direct red 80. An example of a different dye is sirius red 4B (= CI 28160 = Direct red 81), which has been prescribed for use in some staining techniques as a dye with properties similar to those of eosin Y and acid fuchsine. The Biological Stain Commission has standards for sirius red F3B as a collagen stain. Dye from a batch that meets their standards will be OK for your method. My Histonet post from which you are using this method must date from the 1990s, when I knew that picro-sirius red solutions were good for 5-6 years. (I would have written 3 years to be cautious.) With more experience with stored and newly made solutions, I feel confident in saying they keep for more than 20 years. It might get contaminated from too much iron-haematoxylin extracted from previously stained slides. I don't know what this would do. The most obvious cause of red cytoplasmic staining by picrosirus is not enough picric acid (yellow powder in the bottom of the bottle) in the staining solution. It's unfortunate that items found with HistoSearch are undated. It doesn't matter in this case, but many Histonet items become outdated after only a year or two; antibodies and automated staining are examples of fields in which you need to know the age. Keep in touch about your sirius red problem. John Kiernan John A. Kiernan MB, ChB, PhD, DSc Professor Emeritus, Anatomy & Cell Biology University of Western Ontario, London, Canada https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html Also Secretary, Biological Stain Commission, Inc. https://biologicalstaincommission.org = = = ________________________________ From: abi jag via Histonet Sent: 09 August 2019 11:29 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Recent issues with picro sirius red staining (entire liver section become red, no yellow background) Hello Histonetters,I am writing this to seek your help regarding a very recent problem that I am currently facing with Picro Sirius red staining of lab animal (mouse and rat) liver samples. I follow the procedure that was provided by John Kiernan in the histonet archives (please see below), which was working very well. Quite recently, the complete section become stained as red. Usually, collagen in the sections get stained as red with a yellow back ground. Please note that there was no change in the procedure/reagents etc, It will be of great help if you help me in troubleshooting this issue.With my best regards,Abijag Sirius red collagen procedure | | | | Sirius red collagen procedure | | | Solution A. Picro-sirius red Sirius red F3B (C.I. 35782): 0.5 g Saturated aqueous solution of picric acid: 500 ml Add a little solid picric acid to ensure saturation (This is important). (Keeps for at least 3 years and can be used many times.) Solution B. Acidified water Add 5 ml acetic acid (glacial) to 1 litre of water (tap or distilled). Procedure Fixation is not critical, The method is most frequently used on paraffin sections of objects fixed adequately (at least 24 hours but ideally 1 or 2 weeks) in a neutral buffered formaldehyde solution. 1. De-wax and hydrate paraffin sections. 2. (Optional, and not usually done) Stain nuclei with Weigert's haematoxylin (as for the van Gieson method, but more strongly, then wash the slides for 10 minutes in running tap water). 3. Stain in picro-sirius red (Solution A) for one hour. (This gives near-equilibrium staining, which does not increase with longer times. Shorter times should not be used, even if the colours look OK.) 4. Wash in two changes of acidified water (Solution B). 5. Physically remove most of the water from the slides by vigorous shaking or (for a few slides only) blotting with damp filter paper. 5. Dehydrate in three changes of 100% ethanol. 6. Clear in xylene and mount in a resinous medium. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - UT Southwestern Medical Center lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet From michael.gudo at morphisto.de Mon Aug 12 06:28:52 2019 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Mon, 12 Aug 2019 13:28:52 +0200 Subject: [Histonet] Recent issues with picro sirius red staining (entire liver section become red, no yellow background) In-Reply-To: References: <613985784.3074148.1565368162399.ref@mail.yahoo.com> <613985784.3074148.1565368162399@mail.yahoo.com> Message-ID: <6D8DF875-CAE1-45CC-BF27-ACFE93B58D31@morphisto.de> The sirus-red stain is a stain to differentiate collagene I against collagene III in polarized llight, so in bright field all fibres will be red, just if you change to polarised light, you can differentiate both kinds of fibres by their birefrigence which is orange to yellow for collagene 1 and green for the collagene 3. Cheers Michael > Am 10.08.2019 um 06:29 schrieb John Kiernan via Histonet : > > I've never seen the kind of staining you describe, abi jag, with "the complete section become stained as red" but I've never used the method on sections of liver. > > You should get red collagen and yellow hepatocytes with blue nuclei. The strongly acidic picrosirius stain, applied for an hour, always greatly weakens (differentiates) a prior nuclear stain with Weigert's or Lillie's iron-haematoxylin. It's also quite easy to lose some or all the yellow in the washing and dehydration of the stained sections. Most users of this method are interested only in collagen fibres and do not mind if the nuclear and cytoplasmic colours get lost. The iron-haematoxylin nuclear stain is often omitted. > > > It is necessary to have the right dye. It must be sirius red F3B (= CI 35780 = Direct red 80). This is still used as a textile dye, with several suppliers and trade-names. See http://www.worlddyevariety.com/direct-dyes/direct-red-80.html#respond > > Direct Red 80 - worlddyevariety.com > www.worlddyevariety.com > List of Suppliers: Direct Red F3,Direct Fast Red BA,Direct Fast Red F3B. Tianjin Yadong Group . ACDI Red 8 BLN(Aakash Chemicals & Dyestuffs Inc)Alacodirect Red 2BL(Classic Dyestuffs Inc)AmbidirectRed 3BL( Thai Ambica Chemicals Co Ltd) Anadurm Red D-BA( Albion Colours Ltd) Arid Red 8 BLN ( Aashiana Dyestuffs Inc) Best Direct Supra Red F3B( Oriental Giant Dyes and Chemical Ind Corp) > Other dyes with "sirius red" in their trivial names probably are not suitable if they are not the product recognized in the Colour Index as CI 35780, Direct red 80. An example of a different dye is sirius red 4B (= CI 28160 = Direct red 81), which has been prescribed for use in some staining techniques as a dye with properties similar to those of eosin Y and acid fuchsine. > > > The Biological Stain Commission has standards for sirius red F3B as a collagen stain. Dye from a batch that meets their standards will be OK for your method. > > > My Histonet post from which you are using this method must date from the 1990s, when I knew that picro-sirius red solutions were good for 5-6 years. (I would have written 3 years to be cautious.) With more experience with stored and newly made solutions, I feel confident in saying they keep for more than 20 years. > > > It might get contaminated from too much iron-haematoxylin extracted from previously stained slides. I don't know what this would do. > > > The most obvious cause of red cytoplasmic staining by picrosirus is not enough picric acid (yellow powder in the bottom of the bottle) in the staining solution. > > > It's unfortunate that items found with HistoSearch are undated. It doesn't matter in this case, but many Histonet items become outdated after only a year or two; antibodies and automated staining are examples of fields in which you need to know the age. > > Keep in touch about your sirius red problem. > > John Kiernan > John A. Kiernan MB, ChB, PhD, DSc > Professor Emeritus, Anatomy & Cell Biology > University of Western Ontario, London, Canada > https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html > Also Secretary, Biological Stain Commission, Inc. > https://biologicalstaincommission.org > = = = > ________________________________ > From: abi jag via Histonet > Sent: 09 August 2019 11:29 > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Recent issues with picro sirius red staining (entire liver section become red, no yellow background) > > Hello Histonetters,I am writing this to seek your help regarding a very recent problem that I am currently facing with Picro Sirius red staining of lab animal (mouse and rat) liver samples. I follow the procedure that was provided by John Kiernan in the histonet archives (please see below), which was working very well. Quite recently, the complete section become stained as red. Usually, collagen in the sections get stained as red with a yellow back ground. Please note that there was no change in the procedure/reagents etc, It will be of great help if you help me in troubleshooting this issue.With my best regards,Abijag > Sirius red collagen procedure > > | > | > | | > Sirius red collagen procedure > > > | > > | > > | > > > > > Solution A. Picro-sirius red > > Sirius red F3B (C.I. 35782): 0.5 g > Saturated aqueous solution > of picric acid: 500 ml > Add a little solid picric acid to ensure saturation > (This is important). > > (Keeps for at least 3 years and can be used many times.) > > Solution B. Acidified water > > Add 5 ml acetic acid (glacial) to 1 litre of > water (tap or distilled). > > Procedure > > Fixation is not critical, The method is most frequently used on > paraffin sections of objects fixed adequately (at least 24 hours > but ideally 1 or 2 weeks) in a neutral buffered formaldehyde > solution. > > 1. De-wax and hydrate paraffin sections. > 2. (Optional, and not usually done) Stain nuclei with > Weigert's haematoxylin (as for the van Gieson method, > but more strongly, then wash the slides for 10 minutes > in running tap water). > 3. Stain in picro-sirius red (Solution A) for one hour. > (This gives near-equilibrium staining, which does not > increase with longer times. Shorter times should not > be used, even if the colours look OK.) > 4. Wash in two changes of acidified water (Solution B). > 5. Physically remove most of the water from the slides > by vigorous shaking or (for a few slides only) > blotting with damp filter paper. > 5. Dehydrate in three changes of 100% ethanol. > 6. Clear in xylene and mount in a resinous medium. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Histonet Info Page - UT Southwestern Medical Center > lists.utsouthwestern.edu > Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************** MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From kdavoli at gmail.com Mon Aug 12 10:59:06 2019 From: kdavoli at gmail.com (Kate Davoli) Date: Mon, 12 Aug 2019 11:59:06 -0400 Subject: [Histonet] Recent issues with picro sirius red staining (entire liver section become red, no yellow background) In-Reply-To: <6D8DF875-CAE1-45CC-BF27-ACFE93B58D31@morphisto.de> References: <613985784.3074148.1565368162399.ref@mail.yahoo.com> <613985784.3074148.1565368162399@mail.yahoo.com> <6D8DF875-CAE1-45CC-BF27-ACFE93B58D31@morphisto.de> Message-ID: I suspect the "all red" as opposed to "only some red" is because the PSRed is not de-staining out of the non-collagen structures. My guess is that your Solution B is old. Acetic acid becomes weak in water very fast so I make the 1% acetic acid solution fresh every time and use the same day. Hope this helps! On Mon, Aug 12, 2019 at 7:44 AM Dr. Michael Gudo (Morphisto GmbH) via Histonet wrote: > The sirus-red stain is a stain to differentiate collagene I against > collagene III in polarized llight, so in bright field all fibres will be > red, just if you change to polarised light, you can differentiate both > kinds of fibres by their birefrigence which is orange to yellow for > collagene 1 and green for the collagene 3. > > Cheers > Michael > > > > Am 10.08.2019 um 06:29 schrieb John Kiernan via Histonet < > histonet at lists.utsouthwestern.edu>: > > > > I've never seen the kind of staining you describe, abi jag, with "the > complete section become stained as red" but I've never used the method on > sections of liver. > > > > You should get red collagen and yellow hepatocytes with blue nuclei. The > strongly acidic picrosirius stain, applied for an hour, always greatly > weakens (differentiates) a prior nuclear stain with Weigert's or Lillie's > iron-haematoxylin. It's also quite easy to lose some or all the yellow in > the washing and dehydration of the stained sections. Most users of this > method are interested only in collagen fibres and do not mind if the > nuclear and cytoplasmic colours get lost. The iron-haematoxylin nuclear > stain is often omitted. > > > > > > It is necessary to have the right dye. It must be sirius red F3B (= CI > 35780 = Direct red 80). This is still used as a textile dye, with several > suppliers and trade-names. See > http://www.worlddyevariety.com/direct-dyes/direct-red-80.html#respond > > > > Direct Red 80 - worlddyevariety.com< > http://www.worlddyevariety.com/direct-dyes/direct-red-80.html#respond> > > www.worlddyevariety.com > > List of Suppliers: Direct Red F3,Direct Fast Red BA,Direct Fast Red F3B. > Tianjin Yadong Group . ACDI Red 8 BLN(Aakash Chemicals & Dyestuffs > Inc)Alacodirect Red 2BL(Classic Dyestuffs Inc)AmbidirectRed 3BL( Thai > Ambica Chemicals Co Ltd) Anadurm Red D-BA( Albion Colours Ltd) Arid Red 8 > BLN ( Aashiana Dyestuffs Inc) Best Direct Supra Red F3B( Oriental Giant > Dyes and Chemical Ind Corp) > > Other dyes with "sirius red" in their trivial names probably are not > suitable if they are not the product recognized in the Colour Index as CI > 35780, Direct red 80. An example of a different dye is sirius red 4B (= CI > 28160 = Direct red 81), which has been prescribed for use in some staining > techniques as a dye with properties similar to those of eosin Y and acid > fuchsine. > > > > > > The Biological Stain Commission has standards for sirius red F3B as a > collagen stain. Dye from a batch that meets their standards will be OK for > your method. > > > > > > My Histonet post from which you are using this method must date from the > 1990s, when I knew that picro-sirius red solutions were good for 5-6 years. > (I would have written 3 years to be cautious.) With more experience with > stored and newly made solutions, I feel confident in saying they keep for > more than 20 years. > > > > > > It might get contaminated from too much iron-haematoxylin extracted from > previously stained slides. I don't know what this would do. > > > > > > The most obvious cause of red cytoplasmic staining by picrosirus is not > enough picric acid (yellow powder in the bottom of the bottle) in the > staining solution. > > > > > > It's unfortunate that items found with HistoSearch are undated. It > doesn't matter in this case, but many Histonet items become outdated after > only a year or two; antibodies and automated staining are examples of > fields in which you need to know the age. > > > > Keep in touch about your sirius red problem. > > > > John Kiernan > > John A. Kiernan MB, ChB, PhD, DSc > > Professor Emeritus, Anatomy & Cell Biology > > University of Western Ontario, London, Canada > > > https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html > > Also Secretary, Biological Stain Commission, Inc. > > https://biologicalstaincommission.org > > = = = > > ________________________________ > > From: abi jag via Histonet > > Sent: 09 August 2019 11:29 > > To: histonet at lists.utsouthwestern.edu > > > Subject: [Histonet] Recent issues with picro sirius red staining (entire > liver section become red, no yellow background) > > > > Hello Histonetters,I am writing this to seek your help regarding a very > recent problem that I am currently facing with Picro Sirius red staining of > lab animal (mouse and rat) liver samples. I follow the procedure that was > provided by John Kiernan in the histonet archives (please see below), which > was working very well. Quite recently, the complete section become stained > as red. Usually, collagen in the sections get stained as red with a yellow > back ground. Please note that there was no change in the procedure/reagents > etc, It will be of great help if you help me in troubleshooting this > issue.With my best regards,Abijag > > Sirius red collagen procedure > > > > | > > | > > | | > > Sirius red collagen procedure > > > > > > | > > > > | > > > > | > > > > > > > > > > Solution A. Picro-sirius red > > > > Sirius red F3B (C.I. 35782): 0.5 g > > Saturated aqueous solution > > of picric acid: 500 ml > > Add a little solid picric acid to ensure saturation > > (This is important). > > > > (Keeps for at least 3 years and can be used many times.) > > > > Solution B. Acidified water > > > > Add 5 ml acetic acid (glacial) to 1 litre of > > water (tap or distilled). > > > > Procedure > > > > Fixation is not critical, The method is most frequently used on > > paraffin sections of objects fixed adequately (at least 24 hours > > but ideally 1 or 2 weeks) in a neutral buffered formaldehyde > > solution. > > > > 1. De-wax and hydrate paraffin sections. > > 2. (Optional, and not usually done) Stain nuclei with > > Weigert's haematoxylin (as for the van Gieson method, > > but more strongly, then wash the slides for 10 minutes > > in running tap water). > > 3. Stain in picro-sirius red (Solution A) for one hour. > > (This gives near-equilibrium staining, which does not > > increase with longer times. Shorter times should not > > be used, even if the colours look OK.) > > 4. Wash in two changes of acidified water (Solution B). > > 5. Physically remove most of the water from the slides > > by vigorous shaking or (for a few slides only) > > blotting with damp filter paper. > > 5. Dehydrate in three changes of 100% ethanol. > > 6. Clear in xylene and mount in a resinous medium. > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Histonet Info Page - UT Southwestern Medical Center< > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > > lists.utsouthwestern.edu > > Histonet -- For the exchange of information pertaining to > histotechnology and related fields About Histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ******************************************************************************************************** > MORPHISTO GmbH > PD Dr. phil. nat. Michael Gudo > Weism?llerstr. 45 > 60314 Frankfurt am Main > Telefon: 069 / 400 3019 - 62 > Telefax: 069 / 400 3019 - 64 > > E-Mail: michael.gudo at morphisto.de > Internet: http://www.morphisto.de/ > > Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo > > Registergericht: Amtsgericht Frankfurt > Registernummer: HRB 74954 > Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: > DE243397199 > > ************************************************************************************************ > Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder > dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der > unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder > Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht > der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten > wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und > anschliessend diese Email und saemtliche Anhaenge zu loeschen. > > ************************************************************************************************ > This message is exclusively for the person addressed or their > representative. Any form of the unauthorized use, publication, > reproduction, copying or disclosure of the content of this e-mail is not > permitted. If you are not the intended recipient of this message and its > contents, please notify this sender immediately and delete this message and > all its attachments subsequently. > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From galinadeyneko at yahoo.com Mon Aug 12 13:25:32 2019 From: galinadeyneko at yahoo.com (Galina Deyneko) Date: Mon, 12 Aug 2019 18:25:32 +0000 (UTC) Subject: [Histonet] picro sirius red staining References: <696154559.4577432.1565634332978.ref@mail.yahoo.com> Message-ID: <696154559.4577432.1565634332978@mail.yahoo.com> Please see my protocol, we successfully use it on murine liver and hearts.I do not stain nuclei, in my opinion counterstaining with any type of hematoxylin contaminates and fades picris acid. ? -?????????Sirius Red F3BA(Polysciences #09400), (Direct Red 80 from Sigma Chem. Co. (Cat# D 030)) -?????????Saturated aqueouspicric acid solution (LabChem Inc. #LC 18670-2) -?????????Picric acidpowder (Spectrum #P1145) -?????????Phosphomolybdicacid (Sigma #HT153) -?????????Hydrochloric acid(Sigma #H1758) -???? Bouin?solution 0.1% solution of Sirius Red: 0.2 gSirius Red powder 200 mlsaturated aqueous solution of picric acid -?????????Add alittle amount of solid picric acid to ensure saturation. Stir well, filterbefore use. -?????????Stablefor 1 year and can be used many times. ? 02% solution of phosphomolibdic acid: 4 ml of10% phosphomolibdic acid 200 ml DIwater ? 0.01N solution of hydrochloric acid: 166 ?l of38% HCL /830 ?l 200 ml DIwater????? /1 L Procedure: ?????????De-waxSlides ?????????Incubatein 0.2% solution of phosphomolibdic acid (optional) ?????????Mordantovernight in saturated solution of picric acid at room T ?????????Enhancethe yellow color by mordant in saturated solution of picric acid additional 1- 2hour at 60 C in the oven. ?????????Stain in0.1% solution of Sirius Red for 2 hours at RT ?????????Rinse in onechanges of 0.001 N solution HCl- very quick dip!! ?????????Dehydratein four? changes of ?fresh!! 100% Ethanol also quick deep in each ?????????Clearslide through three changes of xylene ?????????Coverslipusing a permanent mounting media Results: The PSRed method stains mostly collagen types I, II, and III. Light microscopy: collagenous fibers ? red, other tissue elements - bright yellowPolarized microscopy:? collagen fibers - orange/red bands against black background ? Galina Deyneko?Novartis, CVM galinadeyneko at yahoo.com From rcharles at pa.gov Tue Aug 13 14:24:41 2019 From: rcharles at pa.gov (Charles, Roger) Date: Tue, 13 Aug 2019 19:24:41 +0000 Subject: [Histonet] BVDV antibody Message-ID: Hello, To all the veterinary labs. Has anyone found an antibody for BVD persistent infection by IHC on ear punch cases. The 15.c.5 clone from IDEXX is no longer available for purchase outside their ELISA test kits. I have a small amount but am going to have change soon. Thanks for all your help Roger Roger Charles| Microbiologist II Pennsylvania Veterinary Laboratory 2305 North Cameron Street | Harrisburg, PA 17110 Phone: 717.787.8808 | Fax: 717.772.3895 www.agriculture.state.pa.us From mcody at seattlecca.org Tue Aug 13 18:19:03 2019 From: mcody at seattlecca.org (Cody, Melissa) Date: Tue, 13 Aug 2019 23:19:03 +0000 Subject: [Histonet] In need of pneumocystis controls, willing to trade! Message-ID: Hello everyone, My lab is in need of pneumocystis controls and we are willing to trade for them! We have iron/liver special stain controls, IHC controls including tonsil as well as lymph nodes that stain for a variety of things. We also have Ventana predilute anti-Vimentin [V9] antibody to trade. Let me know if you are interested! Thank you, Melissa Cody Histologist Seattle Cancer Care Alliance mcody at seattlecca.org 825 Eastlake Ave. E., Mail Stop G7-910 PO Box 19023 Seattle, WA 98109-1023 www.seattlecca.org **Confidentiality Notice** This communication may contain privileged and confidential information intended for the addressee. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of this information (including reliance thereon) is strictly prohibited. If you have received this communication in error, please notify us immediately and then destroy all copies of the communication. To view our complete Notice of Privacy Practices, visit our website at www.seattlecca.org. From akemiat3377 at gmail.com Wed Aug 14 06:05:56 2019 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Wed, 14 Aug 2019 05:05:56 -0600 Subject: [Histonet] recomendations on new cryostats Message-ID: <265E20FC-97AA-4004-8B1E-07C84B044C65@gmail.com> Good Morning Histo Peeps! Our facility is looking to purchase 2 new cryostats. Can any of my pathology/histology colleagues recommend which you prefer. Pros and cons of the Sakura and Leica cryostats would be greatly appreciated. Thank you in advance for your input! Warm regards, Akemi Allison, BS HT/HTL (ASCP) From m.jamison at elitechgroup.com Wed Aug 14 14:23:44 2019 From: m.jamison at elitechgroup.com (Michelle Jamison) Date: Wed, 14 Aug 2019 13:23:44 -0600 Subject: [Histonet] different dehydration methods after staining with PAP Message-ID: <543c4987-fd20-db11-6fdd-b06ad50107db@elitechgroup.com> Hi All with experience with PAP staining, In the last steps of PAP staining there are dehydration steps (with alcohol) then coversliping. Do you ever dehydrate with air, or always with alcohol? Also, do you ever have a problem with moisture being caught under the coverslip causing a cloudy effect? If so, what do you do for that? Last one....if you do dehydrate with alcohol, would you ever consider air dehydration instead? Thanks for your input, Cheers! Michelle -- Best of all things to you, Michelle Jamison // Tel : +1.435.752.6011 Ext. 435.227.1474 m.jamison at elitechgroup.com ? www.elitechgroup.com ------------------------------------------------------------------------ Logo From charles.rowlands at parioforma.com Wed Aug 14 16:22:54 2019 From: charles.rowlands at parioforma.com (Charles Rowlands) Date: Wed, 14 Aug 2019 22:22:54 +0100 Subject: [Histonet] Buy or reagent rental deal for ThinPrep/Surepath Message-ID: <02bb01d552e6$6fa88b10$4ef9a130$@parioforma.com> Hi folks I wondered if anyone could give some ideas on the pros and cons for outright purchase or reagent rental deals for Surepath and Thinprep? How much difference could there be? If your volumes are 6000 might be better to have used deal and buy consumables on market than be tied in? Any benefit if you are a big player with 30,000 runs to buy outright? Any comments insights really welcome? Thanks folks Charles From rmccormick10 at yahoo.com Wed Aug 14 17:10:02 2019 From: rmccormick10 at yahoo.com (Rhonda McCormick) Date: Wed, 14 Aug 2019 16:10:02 -0600 Subject: [Histonet] (no subject) Message-ID: <87B462E6-B875-4E90-A626-4E0655E01EF6@yahoo.com> Help! I became HT certified in 2004 under the HS degree and lab experience qualification. I do have a Bachelor?s Degree in Education,, with only 12 hours of science. I am looking to relocate to Texas and therefore am job hunting. The problem I?m running into is that without an Associates Degree or Bachelors in a Science related field, I am not being considered for job opportunities even though I have 17 years of experience (and am a good histo tech and good employee with great references). Is anyone else running into this problem? Does the Histonet world recommend I go back to school - or just be patient, trusting a job will eventually come along? Thanks! From Timothy.Morken at ucsf.edu Thu Aug 15 09:41:54 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 15 Aug 2019 14:41:54 +0000 Subject: [Histonet] (no subject) In-Reply-To: <87B462E6-B875-4E90-A626-4E0655E01EF6@yahoo.com> References: <87B462E6-B875-4E90-A626-4E0655E01EF6@yahoo.com> Message-ID: Rhonda, that is unfortunate. It seems with the shortage of histotechs in most places that experience would be valued. Certainly you qualify since you were certified under the rules at the time. But institutions can set their own requirements as well which may be more strict. I wonder if your applications are being kicked out by automated programs that simply reject applications if they don't find certain terms. Maybe the managers in the department never even see your application. I have had applicants in this situation and work with my HR department to have them clear such applicants so I can at least get them in the system for consideration even if they don't meet all requirements. Maybe try to find out real people to contact at the institutions pathology department and deliver resumes directly to them rather than thru the online application system they may have. That way they can have their HR send your application through. Tim Morken ________________________________ From: Rhonda McCormick via Histonet Sent: Wednesday, August 14, 2019 3:10:02 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] (no subject) Help! I became HT certified in 2004 under the HS degree and lab experience qualification. I do have a Bachelor?s Degree in Education,, with only 12 hours of science. I am looking to relocate to Texas and therefore am job hunting. The problem I?m running into is that without an Associates Degree or Bachelors in a Science related field, I am not being considered for job opportunities even though I have 17 years of experience (and am a good histo tech and good employee with great references). Is anyone else running into this problem? Does the Histonet world recommend I go back to school - or just be patient, trusting a job will eventually come along? Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=FYWWG7jGOl0t4g1EghKe3VAxRZxp3uuo0eotql28ycc&s=t_zF125Q6vDbaEv5X6SpOMeZTJ0SkJw89MLpH7Z5m8w&e= From relia1 at earthlink.net Thu Aug 15 10:10:34 2019 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 15 Aug 2019 11:10:34 -0400 Subject: [Histonet] Histology Territory Manager opportunities in CA, AZ, OR, NV, WA and CO Message-ID: <00d701d5537b$96451410$c2cf3c30$@earthlink.net> Hello Histopeeps, How are you? I hope this has been a great week and you are looking forward to a fantastic weekend! I was hoping you might be able to help me. I am presently working with a client in the process of building a sales team to cover the western U.S. There are several openings in key states like CO, AZ, CA, WA, OR and WY This is a well-known and well-liked purveyor of histology related products. My client is offering a very competitive compensation package, an excellent support network and an amazing opportunity for an experienced Rep OR someone eager to enter the field!! If you are interested in hearing more about these opportunities please contact me ASAP toll free at 866-607-3542, cell/text 407-353-5070 or via email at relia1 at earthlink.net Histopeeps, if you know anyone that might be interested in hearing about this opportunity could you please forward my e-mail to them? (Remember, if I place someone you refer to me you will earn a referral fee.) Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From may.wei at medaysis.com Thu Aug 15 11:55:24 2019 From: may.wei at medaysis.com (may.wei at medaysis.com) Date: Thu, 15 Aug 2019 09:55:24 -0700 Subject: [Histonet] (no subject) Message-ID: <20190815095524.d841bde8d75ab2d525b632ce17aacdd9.c3596e6f8d.wbe@email03.godaddy.com> Hi Rhonda, To get contact with the local histotech community is a good place to start by contacting local community colleges which offer the histotech certificate (see the list of the local schools [1]https://www.nsh.org/learn/histology-schools). They may even want you to teach one of their lab classes given your extensive experiences. May Wei -------- Original Message -------- Subject: [Histonet] (no subject) From: Rhonda McCormick via Histonet <[2]histonet at lists.utsouthwestern.edu> Date: Wed, August 14, 2019 3:10 pm To: [3]histonet at lists.utsouthwestern.edu Help! I became HT certified in 2004 under the HS degree and lab experience qualification. I do have a Bachelor?s Degree in Education,, with only 12 hours of science. I am looking to relocate to Texas and therefore am job hunting. The problem I?m running into is that without an Associates Degree or Bachelors in a Science related field, I am not being considered for job opportunities even though I have 17 years of experience (and am a good histo tech and good employee with great references). Is anyone else running into this problem? Does the Histonet world recommend I go back to school - or just be patient, trusting a job will eventually come along? Thanks! _______________________________________________ Histonet mailing list [4]Histonet at lists.utsouthwestern.edu [5]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. https://www.nsh.org/learn/histology-schools 2. mailto:histonet at lists.utsouthwestern.edu 3. mailto:histonet at lists.utsouthwestern.edu 4. mailto:Histonet at lists.utsouthwestern.edu 5. http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfields at mlkch.org Thu Aug 15 12:41:45 2019 From: cfields at mlkch.org (Carol G Fields) Date: Thu, 15 Aug 2019 17:41:45 +0000 Subject: [Histonet] Non-Pathologist Grossing Policy - CAP Inspection soon Message-ID: Hi Histotechs, Would someone please share a Policy on PA's or Non-Pathologist's Grossing? Of course this is one of the CAP Checklist questions. Thank you for any help with this, Carole Carole Fields, HT (ASCP) Lead Histotechnologist, Pathology Laboratory Martin Luther King Jr. Community Hospital 1680 E. 120th Street Los Angeles, CA 90059 Fax# 424-296-3932 O:424-338-8000 x 8341 cfields at mlkch.org This email message and any files transmitted are sent with confidentiality in mind and contain privileged or copyright information. You must not present this message to another party without gaining permission from the sender. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. Any views expressed in this message are those of the sender, except where the sender specifically states them to be the views of Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. If you have received this message in error, please notify the sender immediately, and delete this email from your system. We do not guarantee that this material is free from viruses or any other defects although due care has been taken to minimize the risk. From SteveM at mcclainlab.com Thu Aug 15 14:55:25 2019 From: SteveM at mcclainlab.com (Steve McClain) Date: Thu, 15 Aug 2019 19:55:25 +0000 Subject: [Histonet] Histonet Digest, Vol 189, Issue 13 PRAME antibody for melanoma Message-ID: Would one of the LIST members kindly share their experience with PRAM antibody in paraffin/ Steve A. McClain, MD ***************************************** From amurua73 at gmail.com Thu Aug 15 15:56:48 2019 From: amurua73 at gmail.com (Alicia Murua) Date: Thu, 15 Aug 2019 13:56:48 -0700 Subject: [Histonet] Canadian histology work Message-ID: Hello, Is anyone knowledgeable about American ASCP HT certificates and Canadian Histotech requirements? If an American wanted to work in Canada what would be the requirements? From jmacdonald at mtsac.edu Thu Aug 15 16:52:38 2019 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Thu, 15 Aug 2019 21:52:38 +0000 Subject: [Histonet] Canadian histology work In-Reply-To: References: Message-ID: See CSMLS.org Get Outlook for iOS ________________________________ From: Alicia Murua via Histonet Sent: Thursday, August 15, 2019 1:56:48 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Canadian histology work EXTERNAL SENDER- Exercise caution with requests, links, and attachments. Hello, Is anyone knowledgeable about American ASCP HT certificates and Canadian Histotech requirements? If an American wanted to work in Canada what would be the requirements? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 at gmail.com Fri Aug 16 10:00:04 2019 From: akemiat3377 at gmail.com (Akemi) Date: Fri, 16 Aug 2019 09:00:04 -0600 Subject: [Histonet] Respirator Masks for dumping tissue Message-ID: <52E97FB5-725E-420B-8575-834047E4ED37@gmail.com> Hi Histo Peep managers/supervisors: I need to order a few respirators for the staff who dumps tissue. The ones we have are really old and not adequate. Any recommendations would be greatly appreciated. As always, we need to keep within budgetary constants! Have a Great Weekend! Akemi Allison Sent from my iPhone From cfields at mlkch.org Fri Aug 16 10:59:54 2019 From: cfields at mlkch.org (Carol G Fields) Date: Fri, 16 Aug 2019 15:59:54 +0000 Subject: [Histonet] Respirator Masks for dumping tissue In-Reply-To: <52E97FB5-725E-420B-8575-834047E4ED37@gmail.com> References: <52E97FB5-725E-420B-8575-834047E4ED37@gmail.com> Message-ID: Hi, If you are in an area where a company named Stericycle is available, they decant the formalin and dispose of the tissue for you. Our tissue is collected in barrels, they are picked up and Stericycle handles the rest. If you add up your employees time plus all the safety issues involved in the process you may be able to justify this method of disposal. They handle all the waste for several departments in our hospital. This is so much better than exposing employees to this process. Good Luck, Carole Carole Fields, HT (ASCP) Lead Histotechnologist, Pathology Laboratory Martin Luther King Jr. Community Hospital 1680 E. 120th Street Los Angeles, CA 90059 Fax# 424-296-3932 cfields at mlkch.org -----Original Message----- From: Akemi via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, August 16, 2019 8:00 AM To: Histonet Subject: [Histonet] Respirator Masks for dumping tissue Hi Histo Peep managers/supervisors: I need to order a few respirators for the staff who dumps tissue. The ones we have are really old and not adequate. Any recommendations would be greatly appreciated. As always, we need to keep within budgetary constants! Have a Great Weekend! Akemi Allison Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email message and any files transmitted are sent with confidentiality in mind and contain privileged or copyright information. You must not present this message to another party without gaining permission from the sender. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. Any views expressed in this message are those of the sender, except where the sender specifically states them to be the views of Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. If you have received this message in error, please notify the sender immediately, and delete this email from your system. We do not guarantee that this material is free from viruses or any other defects although due care has been taken to minimize the risk. From Joanna.Bartczak at uhn.ca Fri Aug 16 13:01:30 2019 From: Joanna.Bartczak at uhn.ca (Bartczak, Joanna) Date: Fri, 16 Aug 2019 18:01:30 +0000 Subject: [Histonet] Canadian histology work In-Reply-To: References: Message-ID: Hello Alicia, In Canada you must pass the CSMLS exam. If you look up their website (CSMLS.org), you will be able to find all the relevant information, under First Steps to Certification. Also, in Canada, "histotechs" are medical laboratory technologists. Good luck. Joanna Joanna Bartczak Charge Technologist - Immunopathology University Health Network - Toronto General Site 200 Elizabeth Street Toronto, Ontario M5G 2C4 Phone: (416) 340-4800 ext.8037 Joanna.Bartczak at uhn.ca ________________________________ From: Alicia Murua Sent: Thursday, August 15, 2019 4:56 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Canadian histology work Hello, Is anyone knowledgeable about American ASCP HT certificates and Canadian Histotech requirements? If an American wanted to work in Canada what would be the requirements? This e-mail may contain confidential and/or privileged information for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. Opinions, conclusions or other information contained in this e-mail may not be that of the organization. If you feel you have received an email from UHN of a commercial nature and would like to be removed from the sender's mailing list please do one of the following: (1) Follow any unsubscribe process the sender has included in their email (2) Where no unsubscribe process has been included, reply to the sender and type "unsubscribe" in the subject line. If you require additional information please go to our UHN Newsletters and Mailing Lists page. Please note that we are unable to automatically unsubscribe individuals from all UHN mailing lists. From ASelf at tidelandshealth.org Fri Aug 16 13:48:03 2019 From: ASelf at tidelandshealth.org (Amy Self) Date: Fri, 16 Aug 2019 18:48:03 +0000 Subject: [Histonet] CAP Cancer protocols and synoptic reporting Message-ID: Hi Histotechs and Happy Friday, Would someone please share a Policy on the CAP Cancer Protocols and Synoptic Reporting? How are you handling this process? CAP Checklist questions - ANP.12350 Cancer Protocols and ANP.12385 Synoptic Reporting Thank you for any help with this, Amy Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 (843) 520-8711 ASelf at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From amurua73 at gmail.com Fri Aug 16 14:09:14 2019 From: amurua73 at gmail.com (Alicia Murua) Date: Fri, 16 Aug 2019 12:09:14 -0700 Subject: [Histonet] Canadian histology work In-Reply-To: References: Message-ID: Thank you for your help! On Fri, Aug 16, 2019 at 11:07 AM Bartczak, Joanna wrote: > Hello Alicia, > > > In Canada you must pass the CSMLS exam. If you look up their website > (CSMLS.org), you will be able to find all the relevant information, under *First > Steps to Certification*. > > Also, in Canada, "histotechs" are medical laboratory technologists. > > > Good luck. > > Joanna > > > *Joanna > > Bartczak* > > > *Charge Technologist - Immunopathology University Health Network - Toronto > General Site* > > 200 Elizabeth Street > > > Toronto, Ontario M5G 2C4 > > > Phone: (416) 340-4800 ext.8037 > > Joanna.Bartczak at uhn.ca > > > ------------------------------ > *From:* Alicia Murua > *Sent:* Thursday, August 15, 2019 4:56 PM > *To:* Histonet at lists.utsouthwestern.edu > > *Subject:* [Histonet] Canadian histology work > > Hello, > Is anyone knowledgeable about American ASCP HT certificates and Canadian > Histotech requirements? If an American wanted to work in Canada what would > be the requirements? > > > This e-mail may contain confidential and/or privileged information for the > sole use of the intended recipient. > Any review or distribution by anyone other than the person for whom it was > originally intended is strictly prohibited. > If you have received this e-mail in error, please contact the sender and > delete all copies. > Opinions, conclusions or other information contained in this e-mail may > not be that of the organization. > > If you feel you have received an email from UHN of a commercial nature and > would like to be removed from the sender's mailing list please do one of > the following: > (1) Follow any unsubscribe process the sender has included in their email > (2) Where no unsubscribe process has been included, reply to the sender > and type "unsubscribe" in the subject line. If you require additional > information please go to our UHN Newsletters and Mailing Lists page. > Please note that we are unable to automatically unsubscribe individuals > from all UHN mailing lists. > From cfields at mlkch.org Mon Aug 19 10:58:24 2019 From: cfields at mlkch.org (Carol G Fields) Date: Mon, 19 Aug 2019 15:58:24 +0000 Subject: [Histonet] Stericycle question from histonet In-Reply-To: References: Message-ID: Hi, No you just put all your containers in the barrel...sit them in there. They decant the formalin at the company and incinerate the tissue. I've used them at several hospitals. It saves so much time and safety to your employee. Add it all up and see if you break even or maybe ahead. Call them and get a quote. Good luck, Carole From: Richardson, Pam K [mailto:PKRichar at gundersenhealth.org] Sent: Monday, August 19, 2019 5:45 AM To: Carol G Fields Subject: Stericycle question from histonet I have question about your process with Stericycle. Do you put the specimen container and all into or is staff emptying the containers into the barrel? Cordially, Pam ~ National Histology Professionals Day 3/10/19 Pathologist Assistant Day 4/14/2019 Medical Laboratory Professionals Week April 21-27, 2019 National Cytotechnology Day 5/13/2019 +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org This email message and any files transmitted are sent with confidentiality in mind and contain privileged or copyright information. You must not present this message to another party without gaining permission from the sender. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. Any views expressed in this message are those of the sender, except where the sender specifically states them to be the views of Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. If you have received this message in error, please notify the sender immediately, and delete this email from your system. We do not guarantee that this material is free from viruses or any other defects although due care has been taken to minimize the risk. From jwwalker at rrmc.org Mon Aug 19 12:22:07 2019 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Mon, 19 Aug 2019 17:22:07 +0000 Subject: [Histonet] CAP Cancer protocols and synoptic reporting In-Reply-To: References: Message-ID: Hi Amy, We don't have a "policy" per se but we have a process in place to help meet these questions. We utilize Cerner and have created a custom report that looks for cases that require a synoptic report to be used. The report displays cancer cases where a synoptic report was not used. We have to then manually look at these cases and determine the reason why (non-melanoma case, biopsy specimen, etc.) This list is provided to our cancer center and they perform a random 10% audit of these cases to ensure that all of the required elements were completed as required. In order to ensure we are using the current synoptic report, we have an IT analyst who receives the synoptic report updates and updates our templates within the system. As you can see, it is a complex process and not the easiest of tasks. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Amy Self via Histonet Sent: Friday, August 16, 2019 2:48 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] CAP Cancer protocols and synoptic reporting [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. Hi Histotechs and Happy Friday, Would someone please share a Policy on the CAP Cancer Protocols and Synoptic Reporting? How are you handling this process? CAP Checklist questions - ANP.12350 Cancer Protocols and ANP.12385 Synoptic Reporting Thank you for any help with this, Amy Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 (843) 520-8711 ASelf at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7C1c7ed5479982468c0bf208d7227a57a5%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C637015781180506229&sdata=WqpnDLgb4B%2BujzcrIHjKaBy1ReBuVHppLbGsK1mHDDI%3D&reserved=0 [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] From akemiat3377 at gmail.com Tue Aug 20 06:47:23 2019 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Tue, 20 Aug 2019 05:47:23 -0600 Subject: [Histonet] Plus Slides Message-ID: <0EEBE8C8-C8BB-482F-94D5-9A72F60C8811@gmail.com> Good morning Histo Peeps! I would like to get information on where you are getting your Plus Slides for IHC. We are currently getting our (+) charged slides from Cardinal Health and they have little or no charge. We currently have 2 Ventana IHC Instruments, as well a Special Stainer. We are having tissues falling partially and completely off even on the SS Stainer. Also, I realize Ventana will not back the IHC results unless the end user uses a particular brand of (+) charged slides. Years ago when I worked at Biocare Medical I used to QC our Kling-On Slides before selling them by checking to see if the sections indeed stuck to the slides without any lifting on each lot. I realize that positive charged slides have an expiration life, and these slides are newer lot numbers within their expiration. Thank you in advance for your assistance! Akemi Allison, BS, HT/HTL (ASCP) From relia1 at earthlink.net Tue Aug 20 11:42:32 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 20 Aug 2019 12:42:32 -0400 Subject: [Histonet] Mohs Techs paid by case asking for a friend... Message-ID: <00f701d55776$4391e550$cab5aff0$@earthlink.net> Hi Histopeeps, I hope you are having a great day. I have a quick question. A friend of mine would like some information on paying Mohs techs by the case as opposed to hourly. Any info is appreciated. Please feel free to respond directly to me or to the histonet whichever you are more comfortable with. Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From akemiat3377 at gmail.com Wed Aug 21 09:47:27 2019 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Wed, 21 Aug 2019 08:47:27 -0600 Subject: [Histonet] Plus Slides Message-ID: <7A24C39B-CE2D-4D8B-89D1-5A94F14D97FC@gmail.com> Good morning: I just to to thank everyone who responded to my recent inquiry regarding (+) charged slides for IHC. I am getting samples sent to me to test from a few vendors. Thanks again! Akemi Allison, BS, HT/HTL (ASCP) From madeathridge at pastnashville.com Wed Aug 21 11:33:57 2019 From: madeathridge at pastnashville.com (Maryann Deathridge) Date: Wed, 21 Aug 2019 16:33:57 +0000 Subject: [Histonet] HM325 blade holder Message-ID: Histonetters: I am looking for a blade holder for Microm HM325. The microtome works well I just need the blade holder. " I have searched high and search low!" :( Guess I will be looking for a refurbished Microm microtome. Any contacts welcomed. Maryann Deathridge Pathology Assoc. of St. Thomas 4220 Harding Pike Bldg. SE, Suite 504 Nashville, TN 37205 madeathridge at pastnashville.com From cfields at mlkch.org Wed Aug 21 11:48:13 2019 From: cfields at mlkch.org (Carol G Fields) Date: Wed, 21 Aug 2019 16:48:13 +0000 Subject: [Histonet] HM325 blade holder In-Reply-To: References: Message-ID: Call Southeast Pathologist Ins. Phone # 855-731-7999. They are the best! Carole Carole Fields, HT (ASCP) Lead Histotechnologist, Pathology Laboratory Martin Luther King Jr. Community Hospital 1680 E. 120th Street Los Angeles, CA 90059 Fax# 424-296-3932 O:424-338-8000 x 8341 cfields at mlkch.org -----Original Message----- From: Maryann Deathridge via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, August 21, 2019 9:34 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] HM325 blade holder Histonetters: I am looking for a blade holder for Microm HM325. The microtome works well I just need the blade holder. " I have searched high and search low!" :( Guess I will be looking for a refurbished Microm microtome. Any contacts welcomed. Maryann Deathridge Pathology Assoc. of St. Thomas 4220 Harding Pike Bldg. SE, Suite 504 Nashville, TN 37205 madeathridge at pastnashville.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email message and any files transmitted are sent with confidentiality in mind and contain privileged or copyright information. You must not present this message to another party without gaining permission from the sender. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. Any views expressed in this message are those of the sender, except where the sender specifically states them to be the views of Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. If you have received this message in error, please notify the sender immediately, and delete this email from your system. We do not guarantee that this material is free from viruses or any other defects although due care has been taken to minimize the risk. From bcooper at chla.usc.edu Wed Aug 21 12:23:40 2019 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 21 Aug 2019 17:23:40 +0000 Subject: [Histonet] TJP2 antibody Message-ID: <35fdc8b7499a47e0b0c3b44202e9d9e8@chla.usc.edu> Good morning Histonet, Is anyone out there working with TJP2 (tight junction protein) antibody? If so, we'd love to hear from you, and ideally, get a slide or two that demonstrates loss of this protein in human liver. We're willing to provide you with a control block for something else if you can help us out. Pediatric institutions-this one may be in your wheelhouse! Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From Laura.Jones2 at Steward.org Thu Aug 22 08:02:52 2019 From: Laura.Jones2 at Steward.org (Jones, Laura P.) Date: Thu, 22 Aug 2019 13:02:52 +0000 Subject: [Histonet] Antibodies Message-ID: Good Morning. We have changed immunostainers and have lots of antibodies left from our previous one. They are not expired. We would like to give them to a school or research facility who could put them to good use. We would gladly ship them at your expense if anyone is interested. Thanks, Laura Jones Laura Jones Sharon Regional Medical Center, "A Steward Family Hospital" 740 East State Street Sharon, PA 16146 (P) 724-983-3950 Ext. 4875 | (F) 724-983-3982 www.SharonRegionalHealth.org From erin.mccarthy at tempus.com Thu Aug 22 09:41:07 2019 From: erin.mccarthy at tempus.com (Erin McCarthy) Date: Thu, 22 Aug 2019 09:41:07 -0500 Subject: [Histonet] Antibodies In-Reply-To: References: Message-ID: Hi Laura, What stainer did you previously have? I might be interested if it is from either the Roche or Leica platforms as we have both currently at my lab. Also what antibodies? Thank you, On Thu, Aug 22, 2019 at 8:22 AM Jones, Laura P. via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good Morning. We have changed immunostainers and have lots of antibodies > left from our previous one. They are not expired. We would like to give > them to a school or research facility who could put them to good use. We > would gladly ship them at your expense if anyone is interested. > > Thanks, > Laura Jones > > Laura Jones > Sharon Regional Medical Center, "A Steward Family Hospital" > 740 East State Street > Sharon, PA 16146 > (P) 724-983-3950 Ext. 4875 | (F) 724-983-3982 > www.SharonRegionalHealth.org > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Erin McCarthy, HT (ASCP) Histology Supervisor Tempus Labs 600 W. Chicago Ave. Chicago IL 60654 Cell: (708)269-8610 -- This email and any attachments may contain privileged and confidential information and/or protected health information (PHI) that is protected by federal and state privacy laws.? It is intended solely for the use of Tempus Labs and the recipient(s) named above.? Nothing contained in this communication and any attachments thereto is intended to waive any privileges or rights of confidentiality.? If you are not the recipient, or the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any review, dissemination, distribution, printing or copying of this email message and/or any attachments is strictly prohibited.?* If you have received this transmission in error, please notify us immediately at?**(855)-442-8305**? and permanently delete this email and any attachments*. From modz9636 at gmail.com Thu Aug 22 10:22:49 2019 From: modz9636 at gmail.com (M.O.) Date: Thu, 22 Aug 2019 08:22:49 -0700 Subject: [Histonet] tissue blocks stored in cooler C for 2wks - any success with IHC? Message-ID: Good morning! We are working with a tissue bank that is only able to procure tissue blocks from donors that have postmortem intervals of 2wks or more. The storage conditions are in a cooler (I believe 4C), the block will be procured and frozen and we will harvest the tissue from that block once we thaw it. We plan to use these samples for histological purposes only. Has anyone had success with IHC on donors/tissues that were stored in 4C for 2wks or more? Will the integrity of the cells and proteins be okay? Thank you, Merissa From brettmc31 at comcast.net Thu Aug 22 11:14:21 2019 From: brettmc31 at comcast.net (Brett Connolly) Date: Thu, 22 Aug 2019 12:14:21 -0400 Subject: [Histonet] tissue blocks stored in cooler C for 2wks - any successwith IHC? In-Reply-To: References: Message-ID: Merissa, I have done tons of IHC assays on frozen tissues, including many procured from commercial tissue banks. My experience has been that the most of the time the microscopic morphology is terrible (abysmal if it is brain tissue). Cells are always blown open from slow freezing. It?s bad enough that there is a 2 week PMI?add to that storage at 4C instead of at least -20C (best would be -80C) and IMO it?s a recipe for failure. Wouldn?t touch that tissue no way, no how?well maybe if they paid ME I would take it and try to use it for autoradiography or something. Just my opinion. Brett Connolly HTL (ASCP), PhD Sent from Mail for Windows 10 From: M.O. via Histonet Sent: Thursday, August 22, 2019 11:24 AM To: Histonet Subject: [Histonet] tissue blocks stored in cooler C for 2wks - any successwith IHC? Good morning! We are working with a tissue bank that is only able to procure tissue blocks from donors that have postmortem intervals of 2wks or more. The storage conditions are in a cooler (I believe 4C), the block will be procured and frozen and we will harvest the tissue from that block once we thaw it. We plan to use these samples for histological purposes only. Has anyone had success with IHC on donors/tissues that were stored in 4C for 2wks or more? Will the integrity of the cells and proteins be okay? Thank you, Merissa _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aonomic at auburn.edu Thu Aug 22 11:37:08 2019 From: aonomic at auburn.edu (Michelle Aono) Date: Thu, 22 Aug 2019 16:37:08 +0000 Subject: [Histonet] Changing from HemoDe to VWR Xylene Substitute? Message-ID: Hi, Does anyone have any experience changing from HemoDe to VWR Xylene Substitute? Did you have to extend your processing times? By how much? When people talk to good "recycling" of the clearing agent, does that mean re-use? As in how often you need to discard/refill the reservoirs in the processor? I currently coverslip with Eukitt mounting medium, is this compatible? Anything other advice you can offer or things you have discovered during your switch? Thanks! Michelle (Shelly) Aono ~~~~~~~~~~~~~~~~~~~~~~~~ Research Associate II 107B/124 Greene Hall Auburn University, Dept of APP Auburn, AL 36849 (334) 844-5594 From rsrichmond at gmail.com Thu Aug 22 12:56:55 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 22 Aug 2019 13:56:55 -0400 Subject: [Histonet] Changing from HemoDe to VWR Xylene Substitute? In-Reply-To: References: Message-ID: Michelle (Shelly) Aono (at Auburn in Alabama) asks: >>Does anyone have any experience changing from HemoDe to VWR Xylene Substitute? Did you have to extend your processing times? By how much? When people talk to good "recycling" of the clearing agent, does that mean re-use? As in how often you need to discard/refill the reservoirs in the processor? I currently coverslip with Eukitt mounting medium, is this compatible? Anything other advice you can offer or things you have discovered during your switch?<< HemoDe is a limonene clearing agent. I think these are rapidly going out of use. VWR Xylene Substitute is one of a large number of "aliphatic" clearing agents. These are synthetic hydrocarbons, similar to the petroleum cut called naphtha. They are odorless (at least to my insensitive nose), and much less toxic than xylene. They do not all have the same composition, with a wide range of flash points. They are readily recycled, but each one has its own distillation routine (or did with the older generation of recyclers, anyway), so that if you are to recycle, you can't easily change brands, something you need to check with whoever it is in your outfit decides who your vendors are. Bob Richmond Samurai Pathologist Maryville TN From cforster at umn.edu Thu Aug 22 13:26:55 2019 From: cforster at umn.edu (Colleen Forster) Date: Thu, 22 Aug 2019 13:26:55 -0500 Subject: [Histonet] tissue blocks stored in cooler C for 2wks - any successwith IHC? In-Reply-To: References: Message-ID: I agree with Brett. I believe the histological aspect will be horrible and probably not worth the time and money spent. I feel you would be terribly disappointed with the lack of usable results. Colleen Forster HT(ASCP)QIHC On Thu, Aug 22, 2019 at 11:14 AM Brett Connolly via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Merissa, > I have done tons of IHC assays on frozen tissues, including many procured > from commercial tissue banks. My experience has been that the most of the > time the microscopic morphology is terrible (abysmal if it is brain > tissue). Cells are always blown open from slow freezing. It?s bad enough > that there is a 2 week PMI?add to that storage at 4C instead of at least > -20C (best would be -80C) and IMO it?s a recipe for failure. Wouldn?t touch > that tissue no way, no how?well maybe if they paid ME I would take it and > try to use it for autoradiography or something. > Just my opinion. > > Brett Connolly HTL (ASCP), PhD > > Sent from Mail for Windows 10 > > From: M.O. via Histonet > Sent: Thursday, August 22, 2019 11:24 AM > To: Histonet > Subject: [Histonet] tissue blocks stored in cooler C for 2wks - any > successwith IHC? > > Good morning! > > We are working with a tissue bank that is only able to procure tissue > blocks from donors that have postmortem intervals of 2wks or more. The > storage conditions are in a cooler (I believe 4C), the block will be > procured and frozen and we will harvest the tissue from that block once we > thaw it. We plan to use these samples for histological purposes only. > > Has anyone had success with IHC on donors/tissues that were stored in 4C > for 2wks or more? Will the integrity of the cells and proteins be okay? > > Thank you, > Merissa > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at holml at umn.edu * From may.wei at medaysis.com Thu Aug 22 13:30:57 2019 From: may.wei at medaysis.com (may.wei at medaysis.com) Date: Thu, 22 Aug 2019 11:30:57 -0700 Subject: [Histonet] Antibodies Message-ID: <20190822113057.d841bde8d75ab2d525b632ce17aacdd9.f1616a653f.wbe@email03.godaddy.com> Hi Laura, Merritt College of Peralta Community College District ([1]https://www.merritt.edu/wp/histotech/) is interested. See their response below. They are one of the Accredited Schools by State for histology programs. I am a student there. Can you ship FedEx Standard Overnight using my company's (Medaysis Company) FedEx account number 539945947 to: Gisele Giorgi/Feather Ives Merritt College 12500 Campus Drive Oakland, CA 94610 510-384-8527 Please provide the tracking number. Thank you very much for your kindness and support. Look forward to hearing from you. Best regards, May Wei, M.Med., MBA Medaysis Co. 139 E. Airway Blvd. Livermore, CA 94551 Tel: +1 510-509-3153 [2]may.wei at medaysis.com -------- Original Message -------- Subject: Re: [FWD: [Histonet] Antibodies] From: Feather Ives <[3]feathermerritt at gmail.com> Date: Thu, August 22, 2019 11:03 am To: [4]may.wei at medaysis.com Cc: Gisele Giorgi <[5]ggiorgi at peralta.edu> Yes, May! We are interested. Please have them shipped to Gisele Giorgi/Feather Ives 12500 Campus Drive Oakland, CA 94610 510-384-8527 Thank you! On Thu, Aug 22, 2019 at 11:00 AM <[6]may.wei at medaysis.com> wrote: In case Merritt is interested -------- Original Message -------- Subject: [Histonet] Antibodies From: "Jones, Laura P. via Histonet" <[7]histonet at lists.utsouthwestern.edu> Date: Thu, August 22, 2019 6:02 am To: "[8]histonet at lists.utsouthwestern.edu" <[9]histonet at lists.utsouthwestern.edu> Good Morning. We have changed immunostainers and have lots of antibodies left from our previous one. They are not expired. We would like to give them to a school or research facility who could put them to good use. We would gladly ship them at your expense if anyone is interested. Thanks, Laura Jones Laura Jones Sharon Regional Medical Center, "A Steward Family Hospital" 740 East State Street Sharon, PA 16146 (P) 724-983-3950 Ext. 4875 | (F) 724-983-3982 [10]www.SharonRegionalHealth.org<[11]http://www.sharonregionalhealth.or g/> _______________________________________________ Histonet mailing list [12]Histonet at lists.utsouthwestern.edu [13]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. https://www.merritt.edu/wp/histotech/ 2. mailto:may.wei at medaysis.com 3. mailto:feathermerritt at gmail.com 4. mailto:may.wei at medaysis.com 5. mailto:ggiorgi at peralta.edu 6. mailto:may.wei at medaysis.com 7. mailto:histonet at lists.utsouthwestern.edu 8. mailto:histonet at lists.utsouthwestern.edu 9. mailto:histonet at lists.utsouthwestern.edu 10. http://www.SharonRegionalHealth.org/ 11. http://www.sharonregionalhealth.org/ 12. mailto:Histonet at lists.utsouthwestern.edu 13. http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas at biopath.org Fri Aug 23 11:09:41 2019 From: plucas at biopath.org (Paula) Date: Fri, 23 Aug 2019 09:09:41 -0700 Subject: [Histonet] Hologic T2000 error code Message-ID: <002d01d559cd$2c97c7d0$85c75770$@biopath.org> Hello and good day, Can anyone tell me what the error code 76 is? We have 04 as well, and that means the "filter already wet". It comes up right after starting a run so I'm thinking it could be vacuum or waste line issue. Thanks in advance, Paula From Christopher.Sheeder at seattlechildrens.org Fri Aug 23 12:08:55 2019 From: Christopher.Sheeder at seattlechildrens.org (Sheeder, Christopher) Date: Fri, 23 Aug 2019 17:08:55 +0000 Subject: [Histonet] tissue blocks stored in cooler C for 2wks - any successwith IHC? In-Reply-To: References: Message-ID: Totally agree with Brett & Colleen. It is very tempting to have an abundant source of study tissue but the results won't be worth the investment. Best of luck. Chris Christopher Sheeder, HT(ASCP)QIHC Histology Supervisor | Department of Laboratories Seattle Children?s Hospital 4800 Sand Point Way NE Seattle, WA 98105 Office: 206-987-6259 christopher.sheeder at seattlechildrens.org -----Original Message----- From: Colleen Forster Sent: Thursday, August 22, 2019 11:27 AM To: Brett Connolly Cc: M.O. ; Histonet Subject: Re: [Histonet] tissue blocks stored in cooler C for 2wks - any successwith IHC? I agree with Brett. I believe the histological aspect will be horrible and probably not worth the time and money spent. I feel you would be terribly disappointed with the lack of usable results. Colleen Forster HT(ASCP)QIHC On Thu, Aug 22, 2019 at 11:14 AM Brett Connolly via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Merissa, > I have done tons of IHC assays on frozen tissues, including many > procured from commercial tissue banks. My experience has been that the > most of the time the microscopic morphology is terrible (abysmal if it > is brain tissue). Cells are always blown open from slow freezing. It?s > bad enough that there is a 2 week PMI?add to that storage at 4C > instead of at least -20C (best would be -80C) and IMO it?s a recipe > for failure. Wouldn?t touch that tissue no way, no how?well maybe if > they paid ME I would take it and try to use it for autoradiography or something. > Just my opinion. > > Brett Connolly HTL (ASCP), PhD > > Sent from Mail for Windows 10 > > From: M.O. via Histonet > Sent: Thursday, August 22, 2019 11:24 AM > To: Histonet > Subject: [Histonet] tissue blocks stored in cooler C for 2wks - any > successwith IHC? > > Good morning! > > We are working with a tissue bank that is only able to procure tissue > blocks from donors that have postmortem intervals of 2wks or more. The > storage conditions are in a cooler (I believe 4C), the block will be > procured and frozen and we will harvest the tissue from that block > once we thaw it. We plan to use these samples for histological purposes only. > > Has anyone had success with IHC on donors/tissues that were stored in > 4C for 2wks or more? Will the integrity of the cells and proteins be okay? > > Thank you, > Merissa > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=DwIFaQ&c=aBkXpkKi7gN5fe5MqrMaN-VmRu > gaRb1IDRfSv2xVRy0&r=c3bFZxewNEDI2wFyTyj_1jhuE1dV-f-i0gIzQyuiCw3aEzfs6m > Qm_0c0YpHyN0fx&m=swHVgltAOGHSzyaba-zfu9OZ7VT1OCxFCpudUKH7qVY&s=-7ma1dZ > ckaORTpeumgplg6sTkaUhezeV3KpuGyfCu88&e= > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=DwIFaQ&c=aBkXpkKi7gN5fe5MqrMaN-VmRu > gaRb1IDRfSv2xVRy0&r=c3bFZxewNEDI2wFyTyj_1jhuE1dV-f-i0gIzQyuiCw3aEzfs6m > Qm_0c0YpHyN0fx&m=swHVgltAOGHSzyaba-zfu9OZ7VT1OCxFCpudUKH7qVY&s=-7ma1dZ > ckaORTpeumgplg6sTkaUhezeV3KpuGyfCu88&e= > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at holml at umn.edu * CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From slappycraw at yahoo.com Fri Aug 23 13:12:22 2019 From: slappycraw at yahoo.com (Larry Woody) Date: Fri, 23 Aug 2019 18:12:22 +0000 (UTC) Subject: [Histonet] tissue blocks stored in cooler C for 2wks - any successwith IHC? In-Reply-To: References: Message-ID: <1059369617.2073914.1566583942449@mail.yahoo.com> It would have to be stored at -80 to work well otherwise it?s a crap shoot. Sent from Yahoo Mail for iPhone On Friday, August 23, 2019, 10:08 AM, Sheeder, Christopher via Histonet wrote: Totally agree with Brett & Colleen. It is very tempting to have an abundant source of study tissue but the results won't be worth the investment. Best of luck. Chris Christopher Sheeder, HT(ASCP)QIHC Histology Supervisor | Department of Laboratories Seattle Children?s Hospital 4800 Sand Point Way NE Seattle, WA 98105 Office: 206-987-6259 christopher.sheeder at seattlechildrens.org -----Original Message----- From: Colleen Forster Sent: Thursday, August 22, 2019 11:27 AM To: Brett Connolly Cc: M.O. ; Histonet Subject: Re: [Histonet] tissue blocks stored in cooler C for 2wks - any successwith IHC? I agree with Brett. I believe the histological aspect will be horrible and probably not worth the time and money spent. I feel you would be terribly disappointed with the lack of usable results. Colleen Forster HT(ASCP)QIHC On Thu, Aug 22, 2019 at 11:14 AM Brett Connolly via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Merissa, > I have done tons of IHC assays on frozen tissues, including many > procured from commercial tissue banks. My experience has been that the > most of the time the microscopic morphology is terrible (abysmal if it > is brain tissue). Cells are always blown open from slow freezing. It?s > bad enough that there is a 2 week PMI?add to that storage at 4C > instead of at least -20C (best would be -80C) and IMO it?s a recipe > for failure. Wouldn?t touch that tissue no way, no how?well maybe if > they paid ME I would take it and try to use it for autoradiography or something. > Just my opinion. > > Brett Connolly HTL (ASCP), PhD > > Sent from Mail for Windows 10 > > From: M.O. via Histonet > Sent: Thursday, August 22, 2019 11:24 AM > To: Histonet > Subject: [Histonet] tissue blocks stored in cooler C for 2wks - any > successwith IHC? > > Good morning! > > We are working with a tissue bank that is only able to procure tissue > blocks from donors that have postmortem intervals of 2wks or more. The > storage conditions are in a cooler (I believe 4C), the block will be > procured and frozen and we will harvest the tissue from that block > once we thaw it. We plan to use these samples for histological purposes only. > > Has anyone had success with IHC on donors/tissues that were stored in > 4C for 2wks or more? Will the integrity of the cells and proteins be okay? > > Thank you, > Merissa > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=DwIFaQ&c=aBkXpkKi7gN5fe5MqrMaN-VmRu > gaRb1IDRfSv2xVRy0&r=c3bFZxewNEDI2wFyTyj_1jhuE1dV-f-i0gIzQyuiCw3aEzfs6m > Qm_0c0YpHyN0fx&m=swHVgltAOGHSzyaba-zfu9OZ7VT1OCxFCpudUKH7qVY&s=-7ma1dZ > ckaORTpeumgplg6sTkaUhezeV3KpuGyfCu88&e= > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=DwIFaQ&c=aBkXpkKi7gN5fe5MqrMaN-VmRu > gaRb1IDRfSv2xVRy0&r=c3bFZxewNEDI2wFyTyj_1jhuE1dV-f-i0gIzQyuiCw3aEzfs6m > Qm_0c0YpHyN0fx&m=swHVgltAOGHSzyaba-zfu9OZ7VT1OCxFCpudUKH7qVY&s=-7ma1dZ > ckaORTpeumgplg6sTkaUhezeV3KpuGyfCu88&e= > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB? ? ? 612-626-1930 *If submitting histology request please also forward to Lori Holm at holml at umn.edu * CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dawn.Olszewski at SGMC.ORG Fri Aug 23 13:26:52 2019 From: Dawn.Olszewski at SGMC.ORG (Olszewski, Dawn) Date: Fri, 23 Aug 2019 18:26:52 +0000 Subject: [Histonet] Hologic T2000 References: <12a3486f-aba0-466f-9fef-6a4ca11e0a30.3b5c5640-2195-4f35-b5ef-5e1ebee68512.f86656af-6157-4cc1-806a-b607af2f867a@emailsignatures365.codetwo.com> Message-ID: This probably isn't helpful, but I tried to look up your error code in our manual. I did not see code 76 either, but I did see a message that stated if you don't see the code you are looking for, then call Cytyc Technical Service. Dawn Olszewski HTL(ASCP)QIHC Pathology Manager South Georgia Medical Center P: (229) 259-4830 E: dawn.olszewski at sgmc.org Dawn Olszewski Pathology Manager - Histotechnologist Laboratory [cid:MasterLogo_190x61_362365a5-c941-4f16-9f99-9484fd6a6078.png] South Georgia Medical Center 5357 Golf Drive Lake Park, GA 31636 229-259-4830 Dawn.Olszewski at SGMC.ORG | sgmc.org [cid:facebook_fb_32x32_0a6d9065-09e0-48f7-88c4-b2d459a5e547.png] [cid:twitter_32x32_5a3a5eeb-77c7-4a3c-aa09-b69b41f32bd2.png] [cid:linkedin_ln_32x32_bf1e5dc9-b886-480a-8c8b-ef8a1ae87168.png] [cid:youtube_play_32x32_572cd3ed-390b-48e0-8845-ba1f81953de3.png] From Eric_Gilchrist at unc.edu Fri Aug 23 13:49:11 2019 From: Eric_Gilchrist at unc.edu (Gilchrist, Eric P) Date: Fri, 23 Aug 2019 18:49:11 +0000 Subject: [Histonet] Histotechnologist position Message-ID: Looking for Full-Time, Permanent histotechnologist. The position is in the Oral and Maxillofacial Pathology Laboratory at the University of North Carolina Adams School of Dentistry in Chapel Hill. The laboratory is CLIA-certified and receives between 7900 and 8500 accessions per year. Experienced preferred. Interested persons should apply here: https://unc.peopleadmin.com/postings/164836. Eric Gilchrist, Supervisor UNC Oral and Maxillofacial Pathology Laboratory The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. From Karen.Heckford at DignityHealth.org Fri Aug 23 14:03:32 2019 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Fri, 23 Aug 2019 19:03:32 +0000 Subject: [Histonet] Hiring per diem in San Francisco Message-ID: <75ef5c0a267441748a59c63048e4d59d@PHX-EXCH-013.chw.edu> Hi, We are looking for a per diem Histology Technician or HTL with IHC experience. You will be covering the only Histology Tech for Vacations, admin days and sick time. You must be able to work by yourself. Strong troubleshooting skills a plus. We hand stain special stains. The hours are 0430-1230pm Monday through Friday but we may be able to shift some of the hours around if you are working another job. This job could possibly turn into a part time gig down the road. Please go to Dignity Health Online to apply: We are St. Mary's Medical Center in San Francisco. You can call me and leave a message at the number below if you have any questions or respond back to my email address. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you From jqb7 at cdc.gov Mon Aug 26 10:55:18 2019 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)) Date: Mon, 26 Aug 2019 15:55:18 +0000 Subject: [Histonet] Lipid protocol for FFPE tissue Message-ID: Hello all! Does anyone know of a lipid protocol (Sudan Black B, perhaps) that can be used for FFPE tissue? Thanks much! Jeanine Sanders, BS, HT(ASCP), QIHC(ASCP) Centers for Diseases Control and Prevention 1600 Clifton Road NE MS H18-SB Bldg. 18, Rm SB-114 Atlanta, GA 30329 404-639-3590 From Richard.Cartun at hhchealth.org Mon Aug 26 13:48:27 2019 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 26 Aug 2019 18:48:27 +0000 Subject: [Histonet] Tissue Contamination Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EAC186F33@HHCEXCHMB03.hhcsystem.org> What are people doing to ensure that there is no tissue carry-over on instruments between cases when grossing? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From jwwalker at rrmc.org Tue Aug 27 10:36:43 2019 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Tue, 27 Aug 2019 15:36:43 +0000 Subject: [Histonet] Tissue Contamination In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2EAC186F33@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2EAC186F33@HHCEXCHMB03.hhcsystem.org> Message-ID: We utilize small, disposable absorbent pads, which also absorb the formalin fumes. We obtain ours through Leica/former Surgipath. They work well and are changed in between cases. Each case utilizes a new scalpel blade and forceps are rinsed in water between cases. I am not aware of any cross over of tissues between cases when utilizing these practices. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Cartun, Richard via Histonet Sent: Monday, August 26, 2019 2:48 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Tissue Contamination [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. What are people doing to ensure that there is no tissue carry-over on instruments between cases when grossing? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7C101732ac49f34d82e9f408d72a560e09%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C637024421428296021&sdata=2dMuFge6C4Oaev7ZVeLvU1Rdn%2FgohOn2g1wKTJJi%2Fn4%3D&reserved=0 [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] From john.garratt at ciqc.ca Tue Aug 27 11:19:07 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Tue, 27 Aug 2019 16:19:07 +0000 Subject: [Histonet] Tissue Contamination In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2EAC186F33@HHCEXCHMB03.hhcsystem.org> Message-ID: With regard to forceps: Do NOT use rat tooth or serrated forceps because even with rinsing there is potential for micro fragments to be trapped and carried over to the next sample. This also applies to forceps used at the tissue embedding stage. It is all about mitigating of risk. John www.ciqc.ca ??????? Original Message ??????? On Tuesday, August 27, 2019 8:36 AM, Joe W. Walker, Jr. via Histonet wrote: > We utilize small, disposable absorbent pads, which also absorb the formalin fumes. We obtain ours through Leica/former Surgipath. They work well and are changed in between cases. Each case utilizes a new scalpel blade and forceps are rinsed in water between cases. I am not aware of any cross over of tissues between cases when utilizing these practices. > > Joe W. Walker, Jr. MS, SCT(ASCP) > Anatomical Pathology Manager > joewalker at rrmc.org, www.rrmc.org > > -----Original Message----- > From: Cartun, Richard via Histonet histonet at lists.utsouthwestern.edu > Sent: Monday, August 26, 2019 2:48 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Tissue Contamination > > [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. > > What are people doing to ensure that there is no tissue carry-over on instruments between cases when grossing? Thank you. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 (Office) > (860) 545-2204 (Fax) > Richard.cartun at hhchealth.orgmailto:Richard.cartun at hhchealth.org > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02|01|jwwalker%40rrmc.org|101732ac49f34d82e9f408d72a560e09|0e55647d438e4a448437e959c3cf2240|0|0|637024421428296021&sdata=2dMuFge6C4Oaev7ZVeLvU1Rdn%2FgohOn2g1wKTJJi%2Fn4%3D&reserved=0 > [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at dpspa.com Tue Aug 27 11:33:59 2019 From: criley at dpspa.com (Charles Riley) Date: Tue, 27 Aug 2019 12:33:59 -0400 Subject: [Histonet] PRN pay rates Message-ID: What does everyone think is a good PRN rate for an experienced (4+ years) Histotechnician/technologist? Obviously location would play a roll but what do everyone think is a good average rate? And for anyone with knowledge what would a good PRN rate be for a Pathology assistant? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From garreyf at gmail.com Tue Aug 27 12:35:57 2019 From: garreyf at gmail.com (Garrey Faller) Date: Tue, 27 Aug 2019 13:35:57 -0400 Subject: [Histonet] Tissue Contamination In-Reply-To: References: <9215BD4B0BA1B44D962A71C758B68D2EAC186F33@HHCEXCHMB03.hhcsystem.org> Message-ID: <3E9F62C8-500F-44AB-9600-923762959B7A@gmail.com> I agree with the comments made. How do Histotechs mitigate the risk? Do they use water? Do they just place the forceps back into the hot well/holder at the embedder? What is the best way to ensure safe embedding by the Histotech? Although rare, contaminants do end up in blocks. The grosser says it?s the Histotech at fault. The Histotech says it?s the grosser. I once inspected a lab and I witnessed the use of a microbiology flame sterilizer to sterilize the Histotech forceps between biopsies. Never seen that before. Garrey Sent from my iPhone > On Aug 27, 2019, at 12:19 PM, John Garratt via Histonet wrote: > > With regard to forceps: Do NOT use rat tooth or serrated forceps because even with rinsing there is potential for micro fragments to be trapped and carried over to the next sample. This also applies to forceps used at the tissue embedding stage. It is all about mitigating of risk. > > John > > www.ciqc.ca > > ??????? Original Message ??????? >> On Tuesday, August 27, 2019 8:36 AM, Joe W. Walker, Jr. via Histonet wrote: >> >> We utilize small, disposable absorbent pads, which also absorb the formalin fumes. We obtain ours through Leica/former Surgipath. They work well and are changed in between cases. Each case utilizes a new scalpel blade and forceps are rinsed in water between cases. I am not aware of any cross over of tissues between cases when utilizing these practices. >> >> Joe W. Walker, Jr. MS, SCT(ASCP) >> Anatomical Pathology Manager >> joewalker at rrmc.org, www.rrmc.org >> >> -----Original Message----- >> From: Cartun, Richard via Histonet histonet at lists.utsouthwestern.edu >> Sent: Monday, August 26, 2019 2:48 PM >> To: histonet at lists.utsouthwestern.edu >> Subject: [Histonet] Tissue Contamination >> >> [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. >> >> What are people doing to ensure that there is no tissue carry-over on instruments between cases when grossing? Thank you. >> >> Richard >> >> Richard W. Cartun, MS, PhD >> Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital >> 80 Seymour Street >> Hartford, CT 06102 >> (860) 972-1596 (Office) >> (860) 545-2204 (Fax) >> Richard.cartun at hhchealth.orgmailto:Richard.cartun at hhchealth.org >> >> This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. >> >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02|01|jwwalker%40rrmc.org|101732ac49f34d82e9f408d72a560e09|0e55647d438e4a448437e959c3cf2240|0|0|637024421428296021&sdata=2dMuFge6C4Oaev7ZVeLvU1Rdn%2FgohOn2g1wKTJJi%2Fn4%3D&reserved=0 >> [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] >> >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sandra.cheasty at wisc.edu Tue Aug 27 13:14:56 2019 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Tue, 27 Aug 2019 18:14:56 +0000 Subject: [Histonet] 10% NBF Substitutes Message-ID: Hello, Can anyone comment on the current formalin substitutes Excel PLUS and/or FineFIX? -Cost -Benefits -Drawbacks -Performance in tissue processors Thanks! Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From Christopher.Hagon at act.gov.au Tue Aug 27 22:53:08 2019 From: Christopher.Hagon at act.gov.au (Hagon, Christopher (Health)) Date: Wed, 28 Aug 2019 03:53:08 +0000 Subject: [Histonet] Xylene substitutes for clearing agents Message-ID: UNOFFICIAL Hello histonetters! I realise that this has been asked a lot, but cannot find a good link for the comparisons of each. I am charged with looking into converting our lab to go xylene free. We don't want to go down the limonene path, so that leaves the isopropyl alcohol method, or the aliphatic hydrocarbon xylene substitution (Leica Sub-X etc). Looking for opinions from each camp if possible, on how easy or hard it was to change procedures. Was there much trial and error in changing the processing protocols with the aliphatics? Any pitfalls I should look out for? Any input greatly appreciated. Chris Hagon | Senior Scientist, Anatomical Pathology ACT Pathology | health.act.gov.au ----------------------------------------------------------------------- This email, and any attachments, may be confidential and also privileged. If you are not the intended recipient, please notify the sender and delete all copies of this transmission along with any attachments immediately. You should not copy or use it for any purpose, nor disclose its contents to any other person. ----------------------------------------------------------------------- From CIngles at uwhealth.org Wed Aug 28 07:35:22 2019 From: CIngles at uwhealth.org (Ingles Claire) Date: Wed, 28 Aug 2019 12:35:22 +0000 Subject: [Histonet] Xylene substitutes for clearing agents In-Reply-To: References: Message-ID: Chris: Propar from Anatech works great for us. I believe it is still advisable to use xylene in the cleaning cycle on the processors though. We had to go back the other way a bit when our Doc wanted a tape coverslipper. Now he gripes about the xylene smell. Hmmm. Claire ________________________________ From: Hagon, Christopher (Health) via Histonet Sent: Tuesday, August 27, 2019 10:53 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Xylene substitutes for clearing agents WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. UNOFFICIAL Hello histonetters! I realise that this has been asked a lot, but cannot find a good link for the comparisons of each. I am charged with looking into converting our lab to go xylene free. We don't want to go down the limonene path, so that leaves the isopropyl alcohol method, or the aliphatic hydrocarbon xylene substitution (Leica Sub-X etc). Looking for opinions from each camp if possible, on how easy or hard it was to change procedures. Was there much trial and error in changing the processing protocols with the aliphatics? Any pitfalls I should look out for? Any input greatly appreciated. Chris Hagon | Senior Scientist, Anatomical Pathology ACT Pathology | health.act.gov.au ----------------------------------------------------------------------- This email, and any attachments, may be confidential and also privileged. If you are not the intended recipient, please notify the sender and delete all copies of this transmission along with any attachments immediately. You should not copy or use it for any purpose, nor disclose its contents to any other person. ----------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ml at personifysearch.com Wed Aug 28 08:04:17 2019 From: ml at personifysearch.com (Mack Lloyd) Date: Wed, 28 Aug 2019 09:04:17 -0400 Subject: [Histonet] New Job Opportunity - Histology Technician II - Frederick, MD Message-ID: Hi All, We are hiring for a Histology Technician II in Frederick, MD for a Multi-Billion dollar leader in the Research Space. We are looking for individuals with basic histology skills (trimming, embedding, microtomy, staining, etc.) and will be cross training in other areas of the Pathology lab. An HT or HLT license is preferred. This is a growing organization with many advancement opportunities. If you are interested in learning more please let me know contact me at ml at personifysearch.com. Have a great day! Mack Lloyd, Team Lead, Talent Acquisition *Personify* 416 S. Dawson Street Raleigh, NC 27601 Toll Free: (800) 875-6188 ext. 155 Office: 919-694-1125 Cell: 919-815-6009 www.personifysearch.co m ? -- CONFIDENTIALITY NOTICE: ?The contents of this email message and any attachments are intended solely for the addressee(s) and may contain confidential and/or privileged information and may be legally protected from disclosure. ?If you are not the intended recipient of this message or their agent, or if this message has been addressed to you in error, please immediately alert the sender by reply email and then delete this message and any attachments. If you are not the intended recipient, you are hereby notified that any use, dissemination, copying, or storage of this message or its attachments is strictly prohibited. From rjbuesa at yahoo.com Wed Aug 28 08:38:57 2019 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 28 Aug 2019 13:38:57 +0000 (UTC) Subject: [Histonet] Xylene substitutes for clearing agents In-Reply-To: References: Message-ID: <885906680.382290.1566999537255@mail.yahoo.com> Under separate cover I am sending you 3 papers of mine that answer your question.Ren? On Wednesday, August 28, 2019, 08:45:31 AM EDT, Ingles Claire via Histonet wrote: Chris: Propar from Anatech works great for us. I believe it is still advisable to use xylene in the cleaning cycle on the processors though. We had to go back the other way a bit when our Doc wanted a tape coverslipper. Now he gripes about the xylene smell. Hmmm. Claire ________________________________ From: Hagon, Christopher (Health) via Histonet Sent: Tuesday, August 27, 2019 10:53 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Xylene substitutes for clearing agents WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. UNOFFICIAL Hello histonetters! I realise that this has been asked a lot, but cannot find a good link for the comparisons of each. I am charged with looking into converting our lab to go xylene free. We don't want to go down the limonene path, so that leaves the isopropyl alcohol method, or the aliphatic hydrocarbon xylene substitution (Leica Sub-X etc). Looking for opinions from each camp if possible, on how easy or hard it was to change procedures. Was there much trial and error in changing the processing protocols with the aliphatics? Any pitfalls I should look out for? Any input greatly appreciated. Chris Hagon | Senior Scientist, Anatomical Pathology ACT Pathology | health.act.gov.au ----------------------------------------------------------------------- This email, and any attachments, may be confidential and also privileged. If you are not the intended recipient, please notify the sender and delete all copies of this transmission along with any attachments immediately. You should not copy or use it for any purpose, nor disclose its contents to any other person. ----------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From andrea at ka-recruiting.com Wed Aug 28 10:12:14 2019 From: andrea at ka-recruiting.com (Andrea Costello) Date: Wed, 28 Aug 2019 11:12:14 -0400 Subject: [Histonet] Start Fall with a Histology Position Message-ID: Hi Histonetters, I just wanted to reach out to you about some permanent/ full time histology positions that I just got in this week. If you are interested ? you will be one of the first candidates under consideration for these positions and will get a leg up on the competition! If you are interested in any of these positions or exploring a new histology opportunity send your resume to andrea at ka-recruiting.com! *Histotech OR Histology Technician Opening in Phoenix, Arizona!* Histology Technician OR Histotech Opening at a Joint Commission and CAP Accredited Laboratory located in Arizona! This laboratory offers quality results coupled with a rapid turnaround time and is known for unmatched expertise. This laboratory is looking to hire a night shift experienced Histotech. This is a permanent and full time position. For consideration, candidates should have experience in both routine and more complex histology (such as IHC). In addition, applicants must have either a HTL or HT ASCP Certification as well as a BS or AS degree in histology (or related field). I am working on similar positions in *Florida, Illinois, New York, Tennessee, Virginia and Wisconsin!* *Hospital seeks Histotech OR Histology Technician on DAY Shift - Washington, DC area!* Histotech OR Histology Technician Opening at one of Maryland's Top Hospitals! This not-for-profit hospital is nationally ranked in multiple specialties and boasts a research institute, simulation and innovation center and a state-of-the-art laboratory. This hospital is looking to add a permanent and full time histotech on DAY Shift (5a-1:30pm). candidates will have experience in a high volume laboratory environment and have experience with embedding (routine and biopsy) as well as manual stains. Applicants must have completed a Histotechnican (HT) program accredited by NAACLS or an AS degree in a related field. Also, applicants must either have HT (ASCP) or HTL (ASCP) certification I am working on similar positions in *Georgia, New York, North Carolina, North Dakota and Virginia!* Looking forward to hearing from you! Andrea Costello Client Relationship Manager Senior Healthcare Recruiter K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 *P: (617) 746-2745* *F: (617) 507-8009 * andrea at ka-recruiting.com From relia1 at earthlink.net Wed Aug 28 10:39:51 2019 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 28 Aug 2019 11:39:51 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin. 8/28/2019 Opportunities On and Off the Bench! Message-ID: <00bf01d55db6$d4f53b50$7edfb1f0$@earthlink.net> Hi Histonetters! Here are the job opportunities I am excited to share: Histology Leadership Positions Available in: Florida ? Orlando!!! ? Histology Program Instructor ?A RELIA Exclusive! Field Applications Specialist - Chicago and Midwest - A RELIA Exclusive! Histology positions available in: ? Florida Tallahassee Florida Licensure elig. ? Virginia ? Salem Days learn IHC and relo! ? Maryland - Annapolis Days! Great bennies, and relo! ? Arkansas - Fayetteville Days! Great bennies and relo! ? Alabama - Birmingham Night Shift and relo ? California - Concord Days Special Stains expertise! ? Wisconsin ? Milwaukee Days great bennies and sign on bonus! SOME of these are RELIA exclusives MOST of these offer Sign- On Bonuses and/or Relocation Assistance ALL of these Companies offer excellent compensation, benefits and great environments. AND THEY ALL ARE READY TO HIRE!! If You Or Anyone You Know Might Be Interested In Any Of These Positions Or If you or anyone you know would like a customized job search in another area. OR Do you know a travel tech looking to settle down? Please Contact Me! You can reach me by email at relia1 at earthlink.net Toll free at 866-607-3542 or on my cell at 407-353-5070. Have a great day. I look forward to hearing back from you! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From trevorcwicks at hotmail.com Wed Aug 28 12:20:18 2019 From: trevorcwicks at hotmail.com (Trevor Wicks) Date: Wed, 28 Aug 2019 17:20:18 +0000 Subject: [Histonet] Histonet Digest, Vol 189, Issue 24 In-Reply-To: References: Message-ID: Sent from my Samsung device -------- Original message -------- From: histonet-request at lists.utsouthwestern.edu Date: 28/08/2019 18:17 (GMT+00:00) To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 189, Issue 24 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Tissue Contamination (Garrey Faller) 2. 10% NBF Substitutes (Sandra Cheasty) 3. Xylene substitutes for clearing agents (Hagon, Christopher (Health)) 4. Re: Xylene substitutes for clearing agents (Ingles Claire) 5. New Job Opportunity - Histology Technician II - Frederick, MD (Mack Lloyd) 6. Re: Xylene substitutes for clearing agents (Rene J Buesa) 7. Start Fall with a Histology Position (Andrea Costello) 8. RELIA Histology Careers Bulletin. 8/28/2019 Opportunities On and Off the Bench! (Pam Barker) ---------------------------------------------------------------------- Message: 1 Date: Tue, 27 Aug 2019 13:35:57 -0400 From: Garrey Faller To: John Garratt Cc: "Joe W. Walker, Jr." , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Tissue Contamination Message-ID: <3E9F62C8-500F-44AB-9600-923762959B7A at gmail.com> Content-Type: text/plain; charset=utf-8 I agree with the comments made. How do Histotechs mitigate the risk? Do they use water? Do they just place the forceps back into the hot well/holder at the embedder? What is the best way to ensure safe embedding by the Histotech? Although rare, contaminants do end up in blocks. The grosser says it?s the Histotech at fault. The Histotech says it?s the grosser. I once inspected a lab and I witnessed the use of a microbiology flame sterilizer to sterilize the Histotech forceps between biopsies. Never seen that before. Garrey Sent from my iPhone > On Aug 27, 2019, at 12:19 PM, John Garratt via Histonet wrote: > > With regard to forceps: Do NOT use rat tooth or serrated forceps because even with rinsing there is potential for micro fragments to be trapped and carried over to the next sample. This also applies to forceps used at the tissue embedding stage. It is all about mitigating of risk. > > John > > www.ciqc.ca > > ??????? Original Message ??????? >> On Tuesday, August 27, 2019 8:36 AM, Joe W. Walker, Jr. via Histonet wrote: >> >> We utilize small, disposable absorbent pads, which also absorb the formalin fumes. We obtain ours through Leica/former Surgipath. They work well and are changed in between cases. Each case utilizes a new scalpel blade and forceps are rinsed in water between cases. I am not aware of any cross over of tissues between cases when utilizing these practices. >> >> Joe W. Walker, Jr. MS, SCT(ASCP) >> Anatomical Pathology Manager >> joewalker at rrmc.org, www.rrmc.org >> >> -----Original Message----- >> From: Cartun, Richard via Histonet histonet at lists.utsouthwestern.edu >> Sent: Monday, August 26, 2019 2:48 PM >> To: histonet at lists.utsouthwestern.edu >> Subject: [Histonet] Tissue Contamination >> >> [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. >> >> What are people doing to ensure that there is no tissue carry-over on instruments between cases when grossing? Thank you. >> >> Richard >> >> Richard W. Cartun, MS, PhD >> Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital >> 80 Seymour Street >> Hartford, CT 06102 >> (860) 972-1596 (Office) >> (860) 545-2204 (Fax) >> Richard.cartun at hhchealth.orgmailto:Richard.cartun at hhchealth.org >> >> This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. >> >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02|01|jwwalker%40rrmc.org|101732ac49f34d82e9f408d72a560e09|0e55647d438e4a448437e959c3cf2240|0|0|637024421428296021&sdata=2dMuFge6C4Oaev7ZVeLvU1Rdn%2FgohOn2g1wKTJJi%2Fn4%3D&reserved=0 >> [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg] >> >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 27 Aug 2019 18:14:56 +0000 From: Sandra Cheasty To: "Histonet (histonet at lists.utsouthwestern.edu)" Subject: [Histonet] 10% NBF Substitutes Message-ID: Content-Type: text/plain; charset="us-ascii" Hello, Can anyone comment on the current formalin substitutes Excel PLUS and/or FineFIX? -Cost -Benefits -Drawbacks -Performance in tissue processors Thanks! Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ------------------------------ Message: 3 Date: Wed, 28 Aug 2019 03:53:08 +0000 From: "Hagon, Christopher (Health)" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Xylene substitutes for clearing agents Message-ID: Content-Type: text/plain; charset="us-ascii" UNOFFICIAL Hello histonetters! I realise that this has been asked a lot, but cannot find a good link for the comparisons of each. I am charged with looking into converting our lab to go xylene free. We don't want to go down the limonene path, so that leaves the isopropyl alcohol method, or the aliphatic hydrocarbon xylene substitution (Leica Sub-X etc). Looking for opinions from each camp if possible, on how easy or hard it was to change procedures. Was there much trial and error in changing the processing protocols with the aliphatics? Any pitfalls I should look out for? Any input greatly appreciated. Chris Hagon | Senior Scientist, Anatomical Pathology ACT Pathology | health.act.gov.au ----------------------------------------------------------------------- This email, and any attachments, may be confidential and also privileged. If you are not the intended recipient, please notify the sender and delete all copies of this transmission along with any attachments immediately. You should not copy or use it for any purpose, nor disclose its contents to any other person. ----------------------------------------------------------------------- ------------------------------ Message: 4 Date: Wed, 28 Aug 2019 12:35:22 +0000 From: Ingles Claire To: "histonet at lists.utsouthwestern.edu" , "Hagon, Christopher (Health)" Subject: Re: [Histonet] Xylene substitutes for clearing agents Message-ID: Content-Type: text/plain; charset="us-ascii" Chris: Propar from Anatech works great for us. I believe it is still advisable to use xylene in the cleaning cycle on the processors though. We had to go back the other way a bit when our Doc wanted a tape coverslipper. Now he gripes about the xylene smell. Hmmm. Claire ________________________________ From: Hagon, Christopher (Health) via Histonet Sent: Tuesday, August 27, 2019 10:53 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Xylene substitutes for clearing agents WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. UNOFFICIAL Hello histonetters! I realise that this has been asked a lot, but cannot find a good link for the comparisons of each. I am charged with looking into converting our lab to go xylene free. We don't want to go down the limonene path, so that leaves the isopropyl alcohol method, or the aliphatic hydrocarbon xylene substitution (Leica Sub-X etc). Looking for opinions from each camp if possible, on how easy or hard it was to change procedures. Was there much trial and error in changing the processing protocols with the aliphatics? Any pitfalls I should look out for? Any input greatly appreciated. Chris Hagon | Senior Scientist, Anatomical Pathology ACT Pathology | health.act.gov.au ----------------------------------------------------------------------- This email, and any attachments, may be confidential and also privileged. If you are not the intended recipient, please notify the sender and delete all copies of this transmission along with any attachments immediately. You should not copy or use it for any purpose, nor disclose its contents to any other person. ----------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 28 Aug 2019 09:04:17 -0400 From: Mack Lloyd To: histonet at lists.utsouthwestern.edu Subject: [Histonet] New Job Opportunity - Histology Technician II - Frederick, MD Message-ID: Content-Type: text/plain; charset="UTF-8" Hi All, We are hiring for a Histology Technician II in Frederick, MD for a Multi-Billion dollar leader in the Research Space. We are looking for individuals with basic histology skills (trimming, embedding, microtomy, staining, etc.) and will be cross training in other areas of the Pathology lab. An HT or HLT license is preferred. This is a growing organization with many advancement opportunities. If you are interested in learning more please let me know contact me at ml at personifysearch.com. Have a great day! Mack Lloyd, Team Lead, Talent Acquisition *Personify* 416 S. Dawson Street Raleigh, NC 27601 Toll Free: (800) 875-6188 ext. 155 Office: 919-694-1125 Cell: 919-815-6009 www.personifysearch.co m ? -- CONFIDENTIALITY NOTICE: ?The contents of this email message and any attachments are intended solely for the addressee(s) and may contain confidential and/or privileged information and may be legally protected from disclosure. ?If you are not the intended recipient of this message or their agent, or if this message has been addressed to you in error, please immediately alert the sender by reply email and then delete this message and any attachments. If you are not the intended recipient, you are hereby notified that any use, dissemination, copying, or storage of this message or its attachments is strictly prohibited. ------------------------------ Message: 6 Date: Wed, 28 Aug 2019 13:38:57 +0000 (UTC) From: Rene J Buesa To: "histonet at lists.utsouthwestern.edu" , "Hagon, Christopher (Health)" , Ingles Claire Subject: Re: [Histonet] Xylene substitutes for clearing agents Message-ID: <885906680.382290.1566999537255 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Under separate cover I am sending you 3 papers of mine that answer your question.Ren? On Wednesday, August 28, 2019, 08:45:31 AM EDT, Ingles Claire via Histonet wrote: Chris: Propar from Anatech works great for us. I believe it is still advisable to use xylene in the cleaning cycle on the processors though. We had to go back the other way a bit when our Doc wanted a tape coverslipper. Now he gripes about the xylene smell. Hmmm. Claire ________________________________ From: Hagon, Christopher (Health) via Histonet Sent: Tuesday, August 27, 2019 10:53 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Xylene substitutes for clearing agents WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. UNOFFICIAL Hello histonetters! I realise that this has been asked a lot, but cannot find a good link for the comparisons of each. I am charged with looking into converting our lab to go xylene free. We don't want to go down the limonene path, so that leaves the isopropyl alcohol method, or the aliphatic hydrocarbon xylene substitution (Leica Sub-X etc). Looking for opinions from each camp if possible, on how easy or hard it was to change procedures. Was there much trial and error in changing the processing protocols with the aliphatics? Any pitfalls I should look out for? Any input greatly appreciated. Chris Hagon | Senior Scientist, Anatomical Pathology ACT Pathology | health.act.gov.au ----------------------------------------------------------------------- This email, and any attachments, may be confidential and also privileged. If you are not the intended recipient, please notify the sender and delete all copies of this transmission along with any attachments immediately. You should not copy or use it for any purpose, nor disclose its contents to any other person. ----------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 28 Aug 2019 11:12:14 -0400 From: Andrea Costello To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Start Fall with a Histology Position Message-ID: Content-Type: text/plain; charset="UTF-8" Hi Histonetters, I just wanted to reach out to you about some permanent/ full time histology positions that I just got in this week. If you are interested ? you will be one of the first candidates under consideration for these positions and will get a leg up on the competition! If you are interested in any of these positions or exploring a new histology opportunity send your resume to andrea at ka-recruiting.com! *Histotech OR Histology Technician Opening in Phoenix, Arizona!* Histology Technician OR Histotech Opening at a Joint Commission and CAP Accredited Laboratory located in Arizona! This laboratory offers quality results coupled with a rapid turnaround time and is known for unmatched expertise. This laboratory is looking to hire a night shift experienced Histotech. This is a permanent and full time position. For consideration, candidates should have experience in both routine and more complex histology (such as IHC). In addition, applicants must have either a HTL or HT ASCP Certification as well as a BS or AS degree in histology (or related field). I am working on similar positions in *Florida, Illinois, New York, Tennessee, Virginia and Wisconsin!* *Hospital seeks Histotech OR Histology Technician on DAY Shift - Washington, DC area!* Histotech OR Histology Technician Opening at one of Maryland's Top Hospitals! This not-for-profit hospital is nationally ranked in multiple specialties and boasts a research institute, simulation and innovation center and a state-of-the-art laboratory. This hospital is looking to add a permanent and full time histotech on DAY Shift (5a-1:30pm). candidates will have experience in a high volume laboratory environment and have experience with embedding (routine and biopsy) as well as manual stains. Applicants must have completed a Histotechnican (HT) program accredited by NAACLS or an AS degree in a related field. Also, applicants must either have HT (ASCP) or HTL (ASCP) certification I am working on similar positions in *Georgia, New York, North Carolina, North Dakota and Virginia!* Looking forward to hearing from you! Andrea Costello Client Relationship Manager Senior Healthcare Recruiter K.A. Recruiting, Inc. Your Partner in Healthcare Recruiting 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 *P: (617) 746-2745* *F: (617) 507-8009 * andrea at ka-recruiting.com ------------------------------ Message: 8 Date: Wed, 28 Aug 2019 11:39:51 -0400 From: "Pam Barker" To: "Histopeeps Histonet" Subject: [Histonet] RELIA Histology Careers Bulletin. 8/28/2019 Opportunities On and Off the Bench! Message-ID: <00bf01d55db6$d4f53b50$7edfb1f0$@earthlink.net> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters! Here are the job opportunities I am excited to share: Histology Leadership Positions Available in: Florida ? Orlando!!! ? Histology Program Instructor ?A RELIA Exclusive! Field Applications Specialist - Chicago and Midwest - A RELIA Exclusive! Histology positions available in: ? Florida Tallahassee Florida Licensure elig. ? Virginia ? Salem Days learn IHC and relo! ? Maryland - Annapolis Days! Great bennies, and relo! ? Arkansas - Fayetteville Days! Great bennies and relo! ? Alabama - Birmingham Night Shift and relo ? California - Concord Days Special Stains expertise! ? Wisconsin ? Milwaukee Days great bennies and sign on bonus! SOME of these are RELIA exclusives? MOST of these offer Sign- On Bonuses and/or Relocation Assistance ALL of these Companies offer excellent compensation, benefits and great environments. AND THEY ALL ARE READY TO HIRE!! If You Or Anyone You Know Might Be Interested In Any Of These Positions Or If you or anyone you know would like a customized job search in another area. OR Do you know a travel tech looking to settle down? Please Contact Me! You can reach me by email at relia1 at earthlink.net Toll free at 866-607-3542 or on my cell at 407-353-5070. Have a great day. I look forward to hearing back from you! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 189, Issue 24 ***************************************** From akemiat3377 at gmail.com Thu Aug 29 06:21:09 2019 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Thu, 29 Aug 2019 05:21:09 -0600 Subject: [Histonet] Disposal of contaminated gloves, paper towels etc... after grossing Message-ID: Good morning Histo Peeps: I am curious as to your laboratory/hospital policies on how to dispose of contaminated gloves, paper towels etc? after the PA/pathologists gross a specimen. Often times we receive fresh tissue in the lab and we do not know if the patient has TB, Hepatitis, HIV, or any other infectious disease. I was always taught that to treat these items universally as potential biohazards. Do you discard these items in a Biohazard Waste Container,, or do you discard them in a regular trash basket? Thank you in advance for your feedback. Akemi Allison, BS, HT/HTL (ASCP) Histology Manager UMC El Paso, TX From Timothy.Morken at ucsf.edu Thu Aug 29 10:27:36 2019 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 29 Aug 2019 15:27:36 +0000 Subject: [Histonet] Disposal of contaminated gloves, paper towels etc... after grossing In-Reply-To: References: Message-ID: Akemi, all trash in gross room and histo goes into the biohazard waste. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Eileen Akemi Allison via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, August 29, 2019 4:21 AM To: Histonet Subject: [Histonet] Disposal of contaminated gloves, paper towels etc... after grossing Good morning Histo Peeps: I am curious as to your laboratory/hospital policies on how to dispose of contaminated gloves, paper towels etc? after the PA/pathologists gross a specimen. Often times we receive fresh tissue in the lab and we do not know if the patient has TB, Hepatitis, HIV, or any other infectious disease. I was always taught that to treat these items universally as potential biohazards. Do you discard these items in a Biohazard Waste Container,, or do you discard them in a regular trash basket? Thank you in advance for your feedback. Akemi Allison, BS, HT/HTL (ASCP) Histology Manager UMC El Paso, TX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=iORugZls2LlYyCAZRB3XLg&r=7cy9qXFa73jDX2Iixpjkq1XlWAfHgLLHm33agI_sCKA&m=ZeHzaNS4oNsyWryCT2iBEeF-RxMLhHq3AbyULwU6ASg&s=7JPotuLp1-B8Ku4l8XuJZiIsqEHfZl11kM8MF-HT2NI&e= From a.herrera at dbiosys.com Thu Aug 29 10:48:22 2019 From: a.herrera at dbiosys.com (Alejandro Herrera) Date: Thu, 29 Aug 2019 15:48:22 +0000 Subject: [Histonet] RV: Disposal of contaminated gloves, paper towels etc... after grossing In-Reply-To: References: Message-ID: You refer to the classification of bio-infectious hazardous waste? CLASSIFICATION OF R.P.B.I.??? BLACK BAG (DOMESTIC OR MUNICIPAL GARBAGE). Non-infectious waste ? Used paper. ? Syringe wraps, gauze, material, medications. -Kleenex used. RED BAG (CONTAININ BODY FLUIDS) Biological and infectious waste. ? All gloves. ? Syringes with blood or fluids. -Punzocat catheter ? Probes, swabs, gauze. ? Urine collection bags. ? Elastic bandages, plaster. ? Diapers of contaminated patients. ? Venopack, metriset. ? Boots, hats. ? Covers ? Laboratory waste. YELLOW BAG (ANATOMICAL WASTE.) Biological and infectious hazardous waste. ? Surgical piece. -Placenta ? Amputee member. Alejandro Herrera M. Technical Sales Manager Diagnostic BioSystems Tel: 888.896.3350 Cell: +52 1 55 3570 9693 Skype ID: ht.herrera WhatsApp: +52 1 55 3570 9693 Web: www.dbiosys.com ? -----Mensaje original----- De: Eileen Akemi Allison via Histonet Enviado el: Thursday, August 29, 2019 6:21 AM Para: Histonet Asunto: [Histonet] Disposal of contaminated gloves, paper towels etc... after grossing Good morning Histo Peeps: I am curious as to your laboratory/hospital policies on how to dispose of contaminated gloves, paper towels etc? after the PA/pathologists gross a specimen. Often times we receive fresh tissue in the lab and we do not know if the patient has TB, Hepatitis, HIV, or any other infectious disease. I was always taught that to treat these items universally as potential biohazards. Do you discard these items in a Biohazard Waste Container,, or do you discard them in a regular trash basket? Thank you in advance for your feedback. Akemi Allison, BS, HT/HTL (ASCP) Histology Manager UMC El Paso, TX _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From howard at innovativepathology.com Thu Aug 29 15:03:02 2019 From: howard at innovativepathology.com (Howard J. Leventhal) Date: Thu, 29 Aug 2019 16:03:02 -0400 Subject: [Histonet] Tissue Contamination In-Reply-To: References: Message-ID: <008701d55ea4$c45bb690$4d1323b0$@com> I would like to start off by saying that I am a full-time Pathologists' Assistant with 33 years experience. I started a side company about 15 years ago to deal with issues such as this. We currently have a heated forceps jar that by its design greatly reduces the chance of floaters/contaminants. Even more exciting, we have a hands free, patent pending device coming out in the Spring 2020 that will near totally if not completely eliminate the occurrence of contaminants, both at the grossing bench and embedding station. Howard Howard J. Leventhal, M.S., PA(ASCP) Innovative Pathology Concepts, Inc. howard at innovativepathology.com www.innovativepathology.com -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Wednesday, August 28, 2019 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 189, Issue 24 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Tissue Contamination (Garrey Faller) 2. 10% NBF Substitutes (Sandra Cheasty) 3. Xylene substitutes for clearing agents (Hagon, Christopher (Health)) 4. Re: Xylene substitutes for clearing agents (Ingles Claire) 5. New Job Opportunity - Histology Technician II - Frederick, MD (Mack Lloyd) 6. Re: Xylene substitutes for clearing agents (Rene J Buesa) 7. Start Fall with a Histology Position (Andrea Costello) 8. RELIA Histology Careers Bulletin. 8/28/2019 Opportunities On and Off the Bench! (Pam Barker) ---------------------------------------------------------------------- Message: 1 Date: Tue, 27 Aug 2019 13:35:57 -0400 From: Garrey Faller To: John Garratt Cc: "Joe W. Walker, Jr." , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Tissue Contamination Message-ID: <3E9F62C8-500F-44AB-9600-923762959B7A at gmail.com> Content-Type: text/plain; charset=utf-8 I agree with the comments made. How do Histotechs mitigate the risk? Do they use water? Do they just place the forceps back into the hot well/holder at the embedder? What is the best way to ensure safe embedding by the Histotech? Although rare, contaminants do end up in blocks. The grosser says it?s the Histotech at fault. The Histotech says it?s the grosser. I once inspected a lab and I witnessed the use of a microbiology flame sterilizer to sterilize the Histotech forceps between biopsies. Never seen that before. Garrey Sent from my iPhone > On Aug 27, 2019, at 12:19 PM, John Garratt via Histonet wrote: > > With regard to forceps: Do NOT use rat tooth or serrated forceps because even with rinsing there is potential for micro fragments to be trapped and carried over to the next sample. This also applies to forceps used at the tissue embedding stage. It is all about mitigating of risk. > > John > > www.ciqc.ca > > ??????? Original Message ??????? >> On Tuesday, August 27, 2019 8:36 AM, Joe W. Walker, Jr. via Histonet wrote: >> >> We utilize small, disposable absorbent pads, which also absorb the formalin fumes. We obtain ours through Leica/former Surgipath. They work well and are changed in between cases. Each case utilizes a new scalpel blade and forceps are rinsed in water between cases. I am not aware of any cross over of tissues between cases when utilizing these practices. >> >> Joe W. Walker, Jr. MS, SCT(ASCP) >> Anatomical Pathology Manager >> joewalker at rrmc.org, www.rrmc.org >> >> -----Original Message----- >> From: Cartun, Richard via Histonet histonet at lists.utsouthwestern.edu >> Sent: Monday, August 26, 2019 2:48 PM >> To: histonet at lists.utsouthwestern.edu >> Subject: [Histonet] Tissue Contamination >> >> [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. >> >> What are people doing to ensure that there is no tissue carry-over on instruments between cases when grossing? Thank you. >> >> Richard >> >> Richard W. Cartun, MS, PhD >> Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital >> 80 Seymour Street >> Hartford, CT 06102 >> (860) 972-1596 (Office) >> (860) 545-2204 (Fax) >> Richard.cartun at hhchealth.orgmailto:Richard.cartun at hhchealth.org >> >> This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. >> >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsou thwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02|01|jwwalker%40rrmc.org |101732ac49f34d82e9f408d72a560e09|0e55647d438e4a448437e959c3cf2240|0|0|63702 4421428296021&sdata=2dMuFge6C4Oaev7ZVeLvU1Rdn%2FgohOn2g1wKTJJi%2Fn4%3D&reser ved=0 >> [https://www.rrmc.org/app/files/public/2633/2019_hyht_sig-_jan2019_final.jpg ] >> >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 189, Issue 24 ***************************************** From Hans at histologistics.com Fri Aug 30 10:40:01 2019 From: Hans at histologistics.com (Hans B Snyder) Date: Fri, 30 Aug 2019 11:40:01 -0400 Subject: [Histonet] Thermo Cryostar NX Message-ID: Hello All, We have a distributor who is looking for someone that has a Thermo Cryostar and would be willing to test some specimen disks and give feedback. They are willing to give you disks (Free) after testing. If Interested please contact Ken Marzinsky or Helena Murong at kdean70 at hotmail.com, hmurong at springsidesci.com, Phone: 919-864-8821. Thank you Edit Contact Hans B Snyder JMD Histology & Histologistics Inc. 151 W Main Street Dudley, MA 01571 Lab - 508-461-7207 www.histologistics.com -------------------------------------To Err is Human----------------------------------- The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From bakevictoria at gmail.com Fri Aug 30 11:30:48 2019 From: bakevictoria at gmail.com (Victoria Baker) Date: Fri, 30 Aug 2019 12:30:48 -0400 Subject: [Histonet] Roche Symphony and Ultra users Message-ID: Happy Friday! I'm looking to see if anyone out there is using these two specific instruments together. For about 8 or 9 months we have been experiencing what looks like water or water droplets on the IHC slides. It's not on all slides and we can go a span of time with no issues only to have it come back. It's not antibody specific either. We wash (Dawn dish detergent) and then load into the Symphony. I know there is the human factor, but right now I am just looking to see if anyone is or has experienced this. Thanks and enjoy your weekend. Vikki From liz at premierlab.com Fri Aug 30 13:03:04 2019 From: liz at premierlab.com (Liz Chlipala) Date: Fri, 30 Aug 2019 18:03:04 +0000 Subject: [Histonet] Roche Symphony and Ultra users In-Reply-To: References: Message-ID: It might be the coverglass we have seen bubbles in the glass itself, it looks like there is water on the slide, but it's actually bubbles in the coverglass Sent from my Windows 10 device From: Victoria Baker via Histonet Sent: Friday, August 30, 2019 10:44 AM To: Histo Net list server Subject: [Histonet] Roche Symphony and Ultra users Happy Friday! I'm looking to see if anyone out there is using these two specific instruments together. For about 8 or 9 months we have been experiencing what looks like water or water droplets on the IHC slides. It's not on all slides and we can go a span of time with no issues only to have it come back. It's not antibody specific either. We wash (Dawn dish detergent) and then load into the Symphony. I know there is the human factor, but right now I am just looking to see if anyone is or has experienced this. Thanks and enjoy your weekend. Vikki _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From SteveM at mcclainlab.com Fri Aug 30 13:43:25 2019 From: SteveM at mcclainlab.com (Steve McClain) Date: Fri, 30 Aug 2019 18:43:25 +0000 Subject: [Histonet] Histonet Digest, Vol 189, Issue 26 Tissue Contamination /Floaters Message-ID: I wrote and posted on this subject some years ago. In our lab, we virtually eliminated floaters 10 years ago, by using a combination of behavioral and technical procedures: 1) photographing every specimen since 2004 (in order to achieve report -worthy gross images, one must keep the grossing background scrupulously clean for every case). 2) wrapping every specimen in lens paper (creates a double barrier, first keeping floaters from this specimen inside and second keeps potential floaters from other specimens out). 3) folding the lens paper wrapping using forceps (cleaning the forceps as you fold). Thanks, Steve Steve A. McClain, MD