From criley at dpspa.com Tue Apr 2 06:49:56 2019 From: criley at dpspa.com (Charles Riley) Date: Tue, 2 Apr 2019 07:49:56 -0400 Subject: [Histonet] Cell Block trouble shooting Message-ID: Is there any way to save cell blocks that have been rehydrated to much? Tech left them on their ice bath too long and they have become really soft. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From relia1 at earthlink.net Tue Apr 2 10:38:49 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 2 Apr 2019 11:38:49 -0400 Subject: [Histonet] Check out my new hashtag - #jobs4myhistopeeps Message-ID: <000501d4e96a$2b85a110$8290e330$@earthlink.net> Hi Histopeeps, How are you? I hope this is the start to a great week for you! I wanted to share my new hashtag! #jobs4myhistopeeps All of my emails and job postings will be tagged with this hashtag so if you ever want to see what I have going on or want to share it with a friend to earn a referral fee if I place them just search #jobs4myhistopeeps I also have exciting opportunities to share for you and for you to share with your friends! I have been engaged on searches by some of my best clients located in: ? Texas ? North Carolina, ? Virginia ? Tennessee, ? Alabama, ? Georgia ? Alaska ? Ohio ? Wisconsin ? California My clients offer excellent compensation, benefits and in most cases relocation assistance and or sign on bonuses. Here?s what they are looking for: ? ASCP certified or eligible ? Junior, Mid and Senior Level Histologists ? Histotechs looking for Opportunity for Advancement The help I need from you is do you know anyone that might be interested in hearing about any of these opportunities? If so could you please forward my e-mail to them or pass their contact information to me? *remember if I place someone you refer to me you will earn a referral bonus! If you are interested in any of these positions please contact me ASAP on my cell/text 407-353-5070 or toll free at 866-607-3542 or via email at relia1 at earthlink.net If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won?t release it to anyone without your permission. Thanks-Pam #jobs4myhistopeeps Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Blanca.Lopez at UTSouthwestern.edu Tue Apr 2 11:09:02 2019 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Tue, 2 Apr 2019 16:09:02 +0000 Subject: [Histonet] looking for new microtome Message-ID: Hello Histonettes!! Can you help me to find vendors that sales microtomes please? thanks Blanca Lopez HT (ASCP)cm Senior Histotechnologist UT Southwestern Medical Center Harold C. Simmons Comprehensive Cancer Center UTSTR Biorepository Tissue Lab 5323 Harry Hines Blvd K1-210 214-648-7598 blanca.lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From katherine at ka-recruiting.com Wed Apr 3 14:10:44 2019 From: katherine at ka-recruiting.com (Katherine Marano) Date: Wed, 3 Apr 2019 15:10:44 -0400 Subject: [Histonet] new openings in north carolina Message-ID: Hi Histonetters, I hope you are having a nice week. I wanted to let you know about 3 new positions I am looking to fill with a great laboratory outside of Winston-Salem, NC. These are permanent full time positions, and they are all 2nd shift starting at 5pm. If you are interested could you send me a resume and a good time to give you a quick phone call? They are looking to interview asap! HTT ? Histology Technician Trainee HT ? Histology Technician Accessioner Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From Blanca.Lopez at UTSouthwestern.edu Fri Apr 5 10:42:03 2019 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Fri, 5 Apr 2019 15:42:03 +0000 Subject: [Histonet] Microtome point of views Message-ID: <49cc40fef29e4ba9a6b472f196f095ae@SWMS13MAIL12.swmed.org> Morning everybody! I want to ask if anybody had been use the Microtome from Ted Pella HTM 2258... Blanca Lopez HT (ASCP)cm Senior Histotechnologist UT Southwestern Medical Center Harold C. Simmons Comprehensive Cancer Center UTSTR Biorepository Tissue Lab 5323 Harry Hines Blvd K1-210 214-648-7598 blanca.lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From melissa at alliedsearchpartners.com Fri Apr 5 10:57:12 2019 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Fri, 5 Apr 2019 15:57:12 +0000 Subject: [Histonet] PA, HTL/HT, Applications Specialist Job Openings Message-ID: Hello and Happy Friday, Just a quick note on some openings I am seeking candidates for. These are all Full Time/Permanent opportunities. If you are interested or know someone interested please contact me directly for more details. Below are all the ways to contact. Thanks! Northeast Region-New York Pathologist Assistant-Bronx, NY/Day Shift Grosser/Histology-Bronx, NY/2nd Shift 2pm-10:30pm OR 1st Shift/9am-5:30pm Pathologist Assistant-Poughkeepsie, NY/Day Shift (Sign On Bonus) New England Region- Histology Applications Specialist/Boston, MA Mid Atlantic- IHC Applications Specialist/Baltimore, MD Mountain Region: Histotech-Billings, MT/Day Shift Southeast Region: Histotech-Chattanooga, TX/3rd Shift 11am-7:30am Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners Direct (Call) Line: 386.265.1368 Text Me: 386.855.8758 Toll Free: 888.388.7571 ext. 102 Fax: 888.388.7572 From mward at wakehealth.edu Mon Apr 8 12:01:18 2019 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Mon, 8 Apr 2019 17:01:18 +0000 Subject: [Histonet] RUO vs IVD antibodies in immunofluorescence procedures Message-ID: We are looking for IVD antibodies for C1q and C4d for use on frozen tissues (renal). What vendors are folks using for these direct immunofluorescence stains? Thanks in advance for your help. Martha Ward Wake Forest Baptist Health Winston-Salem, NC 27157 From mjdessoye at commonwealthhealth.net Mon Apr 8 13:17:55 2019 From: mjdessoye at commonwealthhealth.net (Dessoye, Michael) Date: Mon, 8 Apr 2019 18:17:55 +0000 Subject: [Histonet] ER/PR/Her2 control Message-ID: Hello Histonet! Looking for some advice regarding ER/PR/Her2 controls. I'm able to buy commercially TMA blocks containing varying levels of positivity for ER, PR, and Her2. This work nicely because you can have a negative and varying levels of positive control as a same-slide control. But our docs would like to score Ki-67 as well. Currently, they are just doing pos/neg for Ki67. It will take some time to collect cases and create a similar control, so I'm wondering what y'all are using? Is there anything commercially available? Or is everybody just collecting cases with varying degrees of positive? Thanks, Mike Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | Commonwealth Health Laboratory Services | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 ********************************************************************** Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From Richard.Cartun at hhchealth.org Mon Apr 8 17:24:18 2019 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 8 Apr 2019 22:24:18 +0000 Subject: [Histonet] H&E Artifact Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EAC12BF35@HHCEXCHMB03.hhcsystem.org> We had several cases last Friday that showed "optically clear nuclei" (primarily lymphocytes) on H&E. Have you seen this artifact and, if so, what is it due do? I will be happy to e-mail a photo to anyone interested. Thank you as always for your help. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From john.garratt at ciqc.ca Mon Apr 8 18:16:19 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Mon, 08 Apr 2019 23:16:19 +0000 Subject: [Histonet] [Histonet) Ki67 control Message-ID: <53VofHYGCNFScPlkqN0SJTr2-t_B2S6DAeMCgQ10h6M17o_Wj-50poDYUPc5MdLU8jAAzvOk8V-vJiZfvWHjThadpKTz25MpLuSiTIbEsJs=@ciqc.ca> My preference, whenever possible, is to steer people towards using normal tissues for controls in IHC. They are consistent in quality and appearance and fixation and processing can be better controlled. I suggest you use Appendix for Ki67 and evaluate the lymphoid follicles plus there are some positive staining cells in the crypts. Should you feel the need to use tumour and quantify the amount staining you could use Mantle Cell Lymphoma. This should give reasonably accurate consistency of Ki67 count through the block. See the article "Standardization of Positive Controls.........." https://www.researchgate.net/publication/282488454_Standardization_of_Positive_Controls_in_Diagnostic_Immunohistochemistry I am most interested in "selling" the concept of iCAPCs ( immunohistochemistry Critical Assay Performance Controls) and any feedback is appreciated. John www.ciqc.ca ??????? Original Message ??????? On Monday, April 8, 2019 11:17 AM, Dessoye, Michael via Histonet wrote: > Hello Histonet! > > Looking for some advice regarding ER/PR/Her2 controls. I'm able to buy commercially TMA blocks containing varying levels of positivity for ER, PR, and Her2. This work nicely because you can have a negative and varying levels of positive control as a same-slide control. But our docs would like to score Ki-67 as well. Currently, they are just doing pos/neg for Ki67. > > It will take some time to collect cases and create a similar control, so I'm wondering what y'all are using? Is there anything commercially available? Or is everybody just collecting cases with varying degrees of positive? > > Thanks, > Mike > > Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | Commonwealth Health Laboratory Services | mjdessoye at commonwealthhealth.netmailto:mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 > > Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Tue Apr 9 10:45:39 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 9 Apr 2019 11:45:39 -0400 Subject: [Histonet] Brainstorming about Relocation and I need your input!! Message-ID: <000001d4eeeb$48471410$d8d53c30$@earthlink.net> Hi Histopeeps, How are you doing today? This e-mail is a little different than the normal e-mail that I send out because I am working on my next article for the NSH newsletter and I need your help. I do have some great jobs but first? Histopeeps, the help I need is this? My article this quarter is about things to consider when considering relocation. I was hoping you might take a second to offer an anecdote or a tip or a hack that I can include as real world experiences along with my advice. I hope you will share your thoughts with me. As always your contributions will be confidential. I am happy to send you a copy of the article once it is written as well. Here are my questions: 1. What made you decide to consider relocation? 2. What are the things you would have done differently if you had it do over again? 3. What are the best and worst things about the move you made? I also have some exciting job opportunities to pass along. Some of these are new and some of them have updated information so please take a look and if you are interested let me know. If you have a friend who is interested and I place them, Then I get to give You a referral reward! I LOVE TO GIVE REFERRAL REWARDS!! SOME of these are RELIA exclusives? MOST of these offer Sign- On Bonuses and/or Relocation Assistance ALL of these Companies offer excellent compensation, benefits and great environments. AND THEY ALL ARE READY TO HIRE!! If You Or Anyone You Know Might Be Interested In Any Of These Positions Or would like a customized job search in another area. Please Contact Me! You can reach me by email at relia1 at earthlink.net Toll free at 866-607-3542 or on my cell at 407-353-5070. Here are the exciting opportunities I am talking about: Spotlight Opportunity A RELIA EXCLUSIVE: Territory Account Manager ? Histology Sales! ? OH, MI, IN, KY, and Western TN!!! Histology Leadership Available in: ? Dallas, TX QA/QC Coordinator Histology A RELIA EXCLUSIVE ? Columbus, OH, MI, IN, KY& TN Histology Sales A RELIA EXCLUSIVE! ? Norfolk, VA $9000K Sign on bonus&relo! Histology positions available in: ? Annapolis, MD Days! Great bennies, and relo! ? Soldotna, AK Days CLIA qualified to gross and relo ? Birmingham, AL Great team, Day and Night Shift and relo ? Modesto CA IHC Specialist State of the art lab, and relo! ? Modesto, CA Routine Histology state of the art lab&relo! ? Charlotte, NC Great place to work! Relo assistance! ? Nashville, TN Grossing Histotech ?days and relo! ? Chattanooga, TN Grossing Histotech ? nights and relo! Have a great day. I look forward to hearing back from you! Thanks-Pam #jobs4myhistopeeps Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From sharon.celligent at gmail.com Wed Apr 10 20:44:42 2019 From: sharon.celligent at gmail.com (sharon.celligent) Date: Wed, 10 Apr 2019 21:44:42 -0400 Subject: [Histonet] 5% Chromic acid disposal Message-ID: We are changing our GMS stain over from a Periodic acid kit to a 5% chromic acid kit. What do the labs that use chromic acid in special staining do with the waste/ used chromic acid?Thanks!Sent via the Samsung Galaxy S7 active, an AT&T 4G LTE smartphone From bill at dornandhart.com Thu Apr 11 10:05:21 2019 From: bill at dornandhart.com (Bill Hart) Date: Thu, 11 Apr 2019 10:05:21 -0500 Subject: [Histonet] New "Polycut Legacy" Microtome In-Reply-To: <3ae975b6-82e9-07a1-ada7-bb1ad211d7d0@dornandhart.com> References: <3ae975b6-82e9-07a1-ada7-bb1ad211d7d0@dornandhart.com> Message-ID: <9ab7f6de-2b30-ad6b-6bdc-c796fba66ae5@dornandhart.com> For anybody interested; there is a new heavy duty sledge microtome being manufactured and coming to market in September. Here is the link for those who are interested: https://www.dornandhart.com/Articles.asp?ID=255 -- Bill Hart Dorn & Hart Microedge, Inc. Bill at dornandhart.com From rsrichmond at gmail.com Thu Apr 11 12:29:09 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 11 Apr 2019 13:29:09 -0400 Subject: [Histonet] 5% Chromic acid disposal In-Reply-To: References: Message-ID: Sharon at Celligent Diagnostics in Spartanburg SC asks: >>We are changing our GMS stain over from a Periodic acid kit to a 5% chromic acid kit. What do the labs that use chromic acid in special staining do with the waste/ used chromic acid?<< I hope someone can give an authoritative answer to this question, with references. Chromic acid (chromium trioxide, CrO3) is a strong oxidant, and chromium is toxic and an environmental hazard. I'd be comfortable with pouring it down the drain with a LOT of water, but what do the authorities want us to do? Bob Richmond Samurai Pathologist Maryville TN From llewllew at shaw.ca Thu Apr 11 13:26:01 2019 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Thu, 11 Apr 2019 11:26:01 -0700 Subject: [Histonet] 5% Chromic acid disposal In-Reply-To: References: Message-ID: <0cabb492-6985-4cb6-0783-172a98cdccf4@shaw.ca> I suspect disposal might vary depending on the State. I live in British Columbia and we had permission from out city (Prince George) to use a dribble tank with lots of water and flush them into the local river (The Fraser), but I opted to collect all the toxic chemicals and ship them periodically to a facility in our neighboring Province of Alberta for recovery and reuse. There must be facilities of that kind somewhere in the United States, I would think. That is probably the best option. We used the same procedure for mercury salts, osmium tetroxide, etc. Bryan Llewellyn Bob Richmond via Histonet wrote: > Sharon at Celligent Diagnostics in Spartanburg SC asks: > >>> We are changing our GMS stain over from a Periodic acid kit to a 5% > chromic acid kit. What do the labs that use chromic acid in special > staining do with the waste/ used chromic acid?<< > > I hope someone can give an authoritative answer to this question, with > references. Chromic acid (chromium trioxide, CrO3) is a strong oxidant, and > chromium is toxic and an environmental hazard. I'd be comfortable with > pouring it down the drain with a LOT of water, but what do the authorities > want us to do? > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tbraud at holyredeemer.com Fri Apr 12 12:31:35 2019 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 12 Apr 2019 17:31:35 +0000 Subject: [Histonet] Chromic Acid Disposal Message-ID: <48E053DDF6CE074DB6A7414BA05403F8015706B39E@HRHEX02-HOS.holyredeemer.local> The best thing to do would be to contact either your city chemist or the state EPA inspector to be sure to be in compliance with all local regulations. A reducing agent, such as sodium metabisulfite, sodium sulfite or sodium thiosulfate can be used to turn CrO3 into the less toxic Cr(III) oxide. Alternatively, I would collect the waste and have a chemical waste disposal company dispose of it. I'm not a big fan of the "flush-it-down-the-sink-with-water" method, unless we are talking minutes amounts. If nothing else, it is about being a better steward of our planet. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Friday, April 12, 2019 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 185, Issue 6 CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links or opening attachments. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: 5% Chromic acid disposal (Bob Richmond) 2. Re: 5% Chromic acid disposal (Bryan Llewellyn) ---------------------------------------------------------------------- Message: 1 Date: Thu, 11 Apr 2019 13:29:09 -0400 From: Bob Richmond To: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] 5% Chromic acid disposal Message-ID: Content-Type: text/plain; charset="UTF-8" Sharon at Celligent Diagnostics in Spartanburg SC asks: >>We are changing our GMS stain over from a Periodic acid kit to a 5% chromic acid kit. What do the labs that use chromic acid in special staining do with the waste/ used chromic acid?<< I hope someone can give an authoritative answer to this question, with references. Chromic acid (chromium trioxide, CrO3) is a strong oxidant, and chromium is toxic and an environmental hazard. I'd be comfortable with pouring it down the drain with a LOT of water, but what do the authorities want us to do? Bob Richmond Samurai Pathologist Maryville TN ------------------------------ Message: 2 Date: Thu, 11 Apr 2019 11:26:01 -0700 From: Bryan Llewellyn To: Histonet Subject: Re: [Histonet] 5% Chromic acid disposal Message-ID: <0cabb492-6985-4cb6-0783-172a98cdccf4 at shaw.ca> Content-Type: text/plain; charset=UTF-8; format=flowed I suspect disposal might vary depending on the State. I live in British Columbia and we had permission from out city (Prince George) to use a dribble tank with lots of water and flush them into the local river (The Fraser), but I opted to collect all the toxic chemicals and ship them periodically to a facility in our neighboring Province of Alberta for recovery and reuse. There must be facilities of that kind somewhere in the United States, I would think. That is probably the best option. We used the same procedure for mercury salts, osmium tetroxide, etc. Bryan Llewellyn Bob Richmond via Histonet wrote: > Sharon at Celligent Diagnostics in Spartanburg SC asks: > >>> We are changing our GMS stain over from a Periodic acid kit to a 5% > chromic acid kit. What do the labs that use chromic acid in special > staining do with the waste/ used chromic acid?<< > > I hope someone can give an authoritative answer to this question, with > references. Chromic acid (chromium trioxide, CrO3) is a strong oxidant, and > chromium is toxic and an environmental hazard. I'd be comfortable with > pouring it down the drain with a LOT of water, but what do the authorities > want us to do? > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 185, Issue 6 **************************************** From aperl at cmmedical.com Fri Apr 12 13:11:42 2019 From: aperl at cmmedical.com (Perl , Alison) Date: Fri, 12 Apr 2019 18:11:42 +0000 Subject: [Histonet] Chromic Acid Disposal In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8015706B39E@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8015706B39E@HRHEX02-HOS.holyredeemer.local> Message-ID: <3d5a6577945841f293ea641c07f204e2@MK-EXMB02.mkmg.com> If you have an account with a waste company for other things, you can certainly ask them - my Stericycle rep has been a great resource. We used to have Clean Harbors/Safety-Kleen, I'm sure they can all help Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager (914) 302-8424 aperl at cmmedical.com -----Original Message----- From: Terri Braud via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, April 12, 2019 1:32 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [EXTERNAL] Re: [Histonet] Chromic Acid Disposal The best thing to do would be to contact either your city chemist or the state EPA inspector to be sure to be in compliance with all local regulations. A reducing agent, such as sodium metabisulfite, sodium sulfite or sodium thiosulfate can be used to turn CrO3 into the less toxic Cr(III) oxide. Alternatively, I would collect the waste and have a chemical waste disposal company dispose of it. I'm not a big fan of the "flush-it-down-the-sink-with-water" method, unless we are talking minutes amounts. If nothing else, it is about being a better steward of our planet. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Friday, April 12, 2019 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 185, Issue 6 CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links or opening attachments. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: 5% Chromic acid disposal (Bob Richmond) 2. Re: 5% Chromic acid disposal (Bryan Llewellyn) ---------------------------------------------------------------------- Message: 1 Date: Thu, 11 Apr 2019 13:29:09 -0400 From: Bob Richmond To: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] 5% Chromic acid disposal Message-ID: Content-Type: text/plain; charset="UTF-8" Sharon at Celligent Diagnostics in Spartanburg SC asks: >>We are changing our GMS stain over from a Periodic acid kit to a 5% chromic acid kit. What do the labs that use chromic acid in special staining do with the waste/ used chromic acid?<< I hope someone can give an authoritative answer to this question, with references. Chromic acid (chromium trioxide, CrO3) is a strong oxidant, and chromium is toxic and an environmental hazard. I'd be comfortable with pouring it down the drain with a LOT of water, but what do the authorities want us to do? Bob Richmond Samurai Pathologist Maryville TN ------------------------------ Message: 2 Date: Thu, 11 Apr 2019 11:26:01 -0700 From: Bryan Llewellyn To: Histonet Subject: Re: [Histonet] 5% Chromic acid disposal Message-ID: <0cabb492-6985-4cb6-0783-172a98cdccf4 at shaw.ca> Content-Type: text/plain; charset=UTF-8; format=flowed I suspect disposal might vary depending on the State. I live in British Columbia and we had permission from out city (Prince George) to use a dribble tank with lots of water and flush them into the local river (The Fraser), but I opted to collect all the toxic chemicals and ship them periodically to a facility in our neighboring Province of Alberta for recovery and reuse. There must be facilities of that kind somewhere in the United States, I would think. That is probably the best option. We used the same procedure for mercury salts, osmium tetroxide, etc. Bryan Llewellyn Bob Richmond via Histonet wrote: > Sharon at Celligent Diagnostics in Spartanburg SC asks: > >>> We are changing our GMS stain over from a Periodic acid kit to a 5% > chromic acid kit. What do the labs that use chromic acid in special > staining do with the waste/ used chromic acid?<< > > I hope someone can give an authoritative answer to this question, with > references. Chromic acid (chromium trioxide, CrO3) is a strong oxidant, and > chromium is toxic and an environmental hazard. I'd be comfortable with > pouring it down the drain with a LOT of water, but what do the authorities > want us to do? > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 185, Issue 6 **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and its attachments, if any, may contain confidential or proprietary information and are intended solely for authorized use by the intended recipient(s) only. Any other use of this email is prohibited. If you have received this email in error, you are hereby notified that any retention, disclosure, copying, forwarding, distribution (in whole or in part and whether electronically, written and/or orally) and/or taking of any action in reliance on this email, its contents and/or any attachments thereto is strictly prohibited. If you received this email in error, please notify the sender by replying to this message and permanently delete this email, and any attachments thereto, from your system immediately. From tgenade at gmail.com Mon Apr 15 10:34:50 2019 From: tgenade at gmail.com (Tyrone Genade) Date: Mon, 15 Apr 2019 11:34:50 -0400 Subject: [Histonet] Chromic Acid Disposal In-Reply-To: References: Message-ID: Hello, For the disposal of Chromic acid see https://www.chemistry.nus.edu.sg/PSSO/safety/Special%20Chemical%20Waste.htm and https://study.com/academy/lesson/chromic-acid-solution-preparation-disposal-hazards.html (more detailed). You can precipitate the chrome as Cr(III) and store this solid for waste disposal instead of liquid chromic acid. In the process the acid is neutralized and the supernatant (of sulfates, potassium and sodium ions) diluted and washed down the sink. I hope that helps. Tyrone From koellingr at comcast.net Mon Apr 15 10:53:17 2019 From: koellingr at comcast.net (RAY KOELLING) Date: Mon, 15 Apr 2019 08:53:17 -0700 (PDT) Subject: [Histonet] Chromic Acid Disposal In-Reply-To: References: Message-ID: <1204306010.239783.1555343598008@connect.xfinity.com> I agree with all these chemical means of disposal or management of wastes and toxic compounds. But hope anyone, as has been mentioned on many occasions by others, please check with your LOCAL waste/sewer/environmental/water entity. Having worked a bit in Florida, California, Washington and Missouri, they don't always agree with one another. And the reference, out of Singapore, is probably at odds with them too. Not only that, but even within a state, county or city rules and regulations can differ from one side of a boundary line to the other. Check locally or you may be suddenly stopped from doing something chemically or environmentally even with the best of your intentions. Ray Koelling lecturer, University of Washington School of Medicine Spokane WWAMI site > On April 15, 2019 at 8:34 AM Tyrone Genade via Histonet wrote: > > > Hello, > > For the disposal of Chromic acid see > https://www.chemistry.nus.edu.sg/PSSO/safety/Special%20Chemical%20Waste.htm > and > https://study.com/academy/lesson/chromic-acid-solution-preparation-disposal-hazards.html > (more > detailed). > > You can precipitate the chrome as Cr(III) and store this solid for waste > disposal instead of liquid chromic acid. In the process the acid is > neutralized and the supernatant (of sulfates, potassium and sodium ions) > diluted and washed down the sink. > > I hope that helps. > > Tyrone > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mgiorgi at incdx.com Mon Apr 15 19:05:10 2019 From: mgiorgi at incdx.com (Miranda Giorgi) Date: Tue, 16 Apr 2019 00:05:10 +0000 Subject: [Histonet] Processing Veterinary Samples Message-ID: Hello, Our AP lab was recently approached by a Veterinary Pathologist to process, embed, and cut H&E slides of their postmortem animal samples. If we consider taking on this work are there any concerns with variation in processing or safety protocols we might need to be aware of with regards to veterinary samples compared to human samples? Any information or experience you can share would be appreciated. Thank you! Miranda Giorgi, HTL (ASCP)cm Histology Manager Incyte Diagnostics 509-892-2744 This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700. From ewj at pigs.ag Tue Apr 16 08:29:59 2019 From: ewj at pigs.ag (e wayne johnson) Date: Tue, 16 Apr 2019 21:29:59 +0800 Subject: [Histonet] Processing Veterinary Samples In-Reply-To: References: Message-ID: <1555421397328.5fddphrkjiffu5iqzkhouwgf@android.mail.163.com> The vet samples can be processed just any other animal tissue whether they be human or rat. People is animals too. Formalin fixed there are no special safety issues. E. Wayne Johnson DVM Enable AgTech Beijing ewj Email:ewj at pigs.ag Signature is customized by Netease Mail Master On 04/16/2019 08:05, Miranda Giorgi via Histonet wrote: Hello, Our AP lab was recently approached by a Veterinary Pathologist to process, embed, and cut H&E slides of their postmortem animal samples. ?If we consider taking on this work are there any concerns with variation in processing or safety protocols we might need to be aware of with regards to veterinary samples compared to human samples? Any information or experience you can share would be appreciated. ?Thank you! Miranda Giorgi, HTL (ASCP)cm Histology Manager Incyte Diagnostics 509-892-2744 This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Monica.Lockhart at luhs.org Tue Apr 16 15:06:52 2019 From: Monica.Lockhart at luhs.org (MONICA D. LOCKHART) Date: Tue, 16 Apr 2019 20:06:52 +0000 Subject: [Histonet] Muscle Enzyme Histochemistry Message-ID: <7aaf58a7933c46f0b24ddf28d6307be0@luhs.org> Hello Histonet!! Hope all is well. My lab would like to replace 2 techniques used during muscle workup with Myosin Fast and Myosin Slow. Is there a lab running these tests that would give us guidance as to how to work these up? Also, we are looking to replace our IDH-1 Antibody by Dianova. Any suggestions? Thanks, Monica D. Lockhart, BBA, HT (ASCP) PBT Supervisor Clinical Labs Histology Loyola University Medical Center 2160 S. First Ave, Bldg 110 Rm 2290 Maywood, IL 60153 (o) 708.327.2608 (c) 708.692.8361 monica.lockhart at luhs.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From relia1 at earthlink.net Wed Apr 17 12:00:37 2019 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 17 Apr 2019 13:00:37 -0400 Subject: [Histonet] ICYMI- In Case You Missed It. Here is part one of my series on Relocation. Message-ID: <000001d4f53f$14fc70a0$3ef551e0$@earthlink.net> Hi Histopeeps, ICYMI - In case you missed it!! I am so excited!! My latest article has been published in the NSH Quarterly Career Center Newsletter under my byline: Make the Cut As you may know I have been writing for the NSH Career newsletter on a quarterly basis and last week in my email I asked for input on my latest topic ? Relocation. The response was so amazing that I am breaking the subject into a 3 part series. The first article part 1 was: Why relocate? Here is the link to the article: https://contentsharing.net/actions/email_web_version.cfm?recipient_id=356670 7953&message_id=16835258&user_id=NSH_&group_id=5370221&jobid=43888461 Part 2 will be in the Summer edition of the Career Newsletter and you will be hearing from me for some input on what you might have done differently when you relocated if you could do it over again. Or Planning for a Relocation. Histopeeps, if you have a minute to read part 1 I would love to hear what you think. If you can?t get to the article with this link let me know and I will send you a copy of it. I also have some exciting job opportunities to pass along. Please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral reward! I LOVE TO GIVE REFERRAL REWARDS!! SOME of these are RELIA exclusives MOST of these offer Sign- on Bonuses and/or Relocation Assistance ALL of these Companies offer excellent compensation, benefits and great environments, opportunity for growth and great people to work with. AND THEY ALL ARE READY TO HIRE!! We have amazing opportunities nationwide! Here they are! Histology Spotlight Opportunity: Histology Territory Sales Manager Ohio Marketing/Sales Representative for Ohio and part of the rest of the Midwest region (MI, OH, IN, KY and Western TN).? Strong histology and leadership skills are required and previous sales experience is a plus. This is a well-known and well-liked purveyor of histology related products.? My client is offering a very competitive compensation package, an excellent support network and an amazing opportunity for an experienced Rep OR someone eager to enter the field!! I also have exciting histology opportunities in the following areas: Western MA (close to NY border!)- No NY LICENSE required!! Maryland North Carolina Virginia Texas Tennessee California Alabama My clients offer competitive pay rates, excellent benefits and in most cases Relocation assistance or a sign on bonus. They are ready to interview and hire!!! What more could you ask for? If You or Anyone You Know Might Be Interested In a New Opportunity, Please Contact Me ASAP!! Even if you are just mildly interested for yourself or a friend shoot me a quick email for the details!! If you want to chat ASAP call or text me on my cell At 407-353-5070. If you want some additional information or to set up a time to chat please call me toll free at 866-607-3542 or email me at relia1 at earthlink.net My clients are ready to interview and hire right away!!! And my phone is still ringing off the hook so if you don?t see the location you want yet drop me a line so I can let you know when something DOES come up. I could be talking to a client about your next opportunity RIGHT NOW!! Have a great day. I look forward to hearing back from you. Thanks-Pam #jobs4myhistopeeps Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From criley at dpspa.com Thu Apr 18 07:41:29 2019 From: criley at dpspa.com (Charles Riley) Date: Thu, 18 Apr 2019 08:41:29 -0400 Subject: [Histonet] Lab Week Message-ID: Anyone have any ideas on things I can do in our lab to celebrate lab week? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From relia1 at earthlink.net Thu Apr 18 08:29:13 2019 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 18 Apr 2019 09:29:13 -0400 Subject: [Histonet] Lab Week In-Reply-To: References: Message-ID: <005a01d4f5ea$b6916000$23b42000$@earthlink.net> Hi Charles! I hope you are doing well. Here is some info for Lab Week. Many labs are celebrating with trivia contests, luncheons, themed days and general activities to promote their professions. If you get a chance check out the ASCP?s website section for Lab week. Here is the link: http://www.ascp.org/labweek Thanks-Pam #jobs4myhistopeeps Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, April 18, 2019 8:41 AM To: Histo List Subject: [Histonet] Lab Week Anyone have any ideas on things I can do in our lab to celebrate lab week? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen at parrishmed.com Thu Apr 18 09:05:08 2019 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Thu, 18 Apr 2019 10:05:08 -0400 Subject: [Histonet] [External Sender] Lab Week In-Reply-To: References: Message-ID: <450B7A81EDA0C54E97C53D60F00776C3243D9ECE57@isexstore03> In addition to the fun ideas shared by others.. we also do cooking contests with prizes!! We do "cake walks" for our staff with the Section Chiefs/ supervisors providing the goody for the cake walk... the staff love it, they say it helps cheer up their day... thinking maybe they will be the one to win the sweet treat!! We (supervisors) also make up little gift bags for our staff to show our appreciation for all of their hard work!! Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, April 18, 2019 8:41 AM To: Histo List Subject: [External Sender] [Histonet] Lab Week WARNING: This message came from an external source. Please do not click links or open attachments if unexpected or unusual. Anyone have any ideas on things I can do in our lab to celebrate lab week? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From srishan at mail.holyname.org Thu Apr 18 11:15:10 2019 From: srishan at mail.holyname.org (Nirmala Srishan) Date: Thu, 18 Apr 2019 12:15:10 -0400 Subject: [Histonet] her 2 controls Message-ID: Can someone suggest a company to purchase good Her 2 control slides. Preferably multi-tissue controls with +1,+2,+3. Thanks in advance. Nirmala Srishan Supervisor Histology Department of Pathology and Laboratory Medicine Holy Name Medical Center Teaneck, NJ Office: 201 541 6328 Lab: 201 833 3023 srishan at holyname.org Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. From john.garratt at ciqc.ca Thu Apr 18 12:06:58 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 18 Apr 2019 17:06:58 +0000 Subject: [Histonet] her 2 controls In-Reply-To: References: Message-ID: <_YCHVevdPa_YX2zud8v_MCgbQwG0xUocsLWHC5vSbyqsDzR9ZVgr_Ct9K_5xkEp1g-2zKDTiDDAtEz48DHGPD0187h6dEyR54oiWhihq52E=@ciqc.ca> I recommend that you e-mail these two gentlemen (both are super helpful) and they can put you in touch with your local distributor. Both their companies have HER2 controls plus controls for many other analytes. MRees at statlab.com (Mark Rees) colin.tristram at histocyte.com John www.ciqc.ca ??????? Original Message ??????? On Thursday, April 18, 2019 9:15 AM, Nirmala Srishan via Histonet wrote: > Can someone suggest a company to purchase good Her 2 control slides. > Preferably multi-tissue controls with +1,+2,+3. > Thanks in advance. > > Nirmala Srishan > Supervisor Histology > Department of Pathology and Laboratory Medicine > Holy Name Medical Center Teaneck, NJ > Office: 201 541 6328 > Lab: 201 833 3023 > srishan at holyname.org > > Holy Name Medical Center is ranked among the top hospitals in the nation > for patient care, clinical performance and workplace excellence. > Click here to learn more. > > **** Warning: The information contained in this message is privileged and > CONFIDENTIAL and is intended only for the use of the addressee above. If > you are not the intended recipient, you are hereby notified that any > disclosure, copying, distribution, or taking of any action in reliance on > the content of this message is strictly prohibited. If you have received > this communication in error, please notify the sender by replying to this > message, and then delete it from your system. > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mjdessoye at commonwealthhealth.net Thu Apr 18 12:35:23 2019 From: mjdessoye at commonwealthhealth.net (Dessoye, Michael) Date: Thu, 18 Apr 2019 17:35:23 +0000 Subject: [Histonet] her 2 controls In-Reply-To: References: Message-ID: Check out Histocyte controls, which are available on slides or blocks. One of their distributors is Cell Marque: http://www.cellmarque.com/cms/histocyte/HER2.php Their Her2 control include 0, 1+, 2+, and 3+. Michael J. Dessoye, M.S.?|?Histology/Toxicology/Special Chemistry Supervisor?|?Commonwealth Health Laboratory Services |?mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 -----Original Message----- From: Nirmala Srishan [mailto:srishan at mail.holyname.org] Sent: Thursday, April 18, 2019 12:15 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] her 2 controls Can someone suggest a company to purchase good Her 2 control slides. Preferably multi-tissue controls with +1,+2,+3. Thanks in advance. Nirmala Srishan Supervisor Histology Department of Pathology and Laboratory Medicine Holy Name Medical Center Teaneck, NJ Office: 201 541 6328 Lab: 201 833 3023 srishan at holyname.org Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ********************************************************************** Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From denise.long at uconn.edu Fri Apr 19 14:17:08 2019 From: denise.long at uconn.edu (Long, Denise) Date: Fri, 19 Apr 2019 19:17:08 +0000 Subject: [Histonet] Cassette Printers: Leica vs Sakura vs Thermo Message-ID: Hi all, We're looking at purchasing a new cassette printer. Looking at Leica, Sakura and Thermo models. I'd love to hear good and bad feedback on any model you might be using. You can send your comments privately. Thanks in advance, Denise M Long Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 From victor_tobias at comcast.net Fri Apr 19 15:06:47 2019 From: victor_tobias at comcast.net (Victor Tobias) Date: Fri, 19 Apr 2019 13:06:47 -0700 Subject: [Histonet] Cassette Printers: Leica vs Sakura vs Thermo Message-ID: <583c6694-540a-4254-9486-67b8ec00870e@Vics-iPhone> Denise, Don?t forget General Data. I?m retired now, but we had about 10 of them. Real workhorse, fastest printer at the time and very reliable. Victor Sent from XFINITY Connect Mobile App ------ Original Message ------ From: Long, Denise via Histonet To: histonet at lists.utsouthwestern.edu Sent: April 19, 2019 at 12:18 PM Subject: [Histonet] Cassette Printers: Leica vs Sakura vs Thermo Hi all, We're looking at purchasing a new cassette printer. Looking at Leica, Sakura and Thermo models. I'd love to hear good and bad feedback on any model you might be using. You can send your comments privately. Thanks in advance, Denise M Long Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TWELLEN at LHS.ORG Fri Apr 19 18:45:47 2019 From: TWELLEN at LHS.ORG (Wellen, Terry :LLS Lab) Date: Fri, 19 Apr 2019 23:45:47 +0000 Subject: [Histonet] CK Pools Message-ID: Would anybody share which antibodies they use for their CK Pools? Some questions have been raised by some of our newer Pathologists. Thanks, Terrence D. Wellen HT (ASCP) Technical Specialist Legacy Laboratory Pathology Legacy Central Lab 1225 N.E. 2nd Ave. Portland, OR 972322003 503-944-7924 From cforster at umn.edu Fri Apr 19 20:38:04 2019 From: cforster at umn.edu (Colleen Forster) Date: Fri, 19 Apr 2019 20:38:04 -0500 Subject: [Histonet] CK Pools In-Reply-To: References: Message-ID: Following... On Friday, April 19, 2019, Wellen, Terry :LLS Lab via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Would anybody share which antibodies they use for their CK Pools? > Some questions have been raised by some of our newer Pathologists. > > > Thanks, > > Terrence D. Wellen HT (ASCP) > Technical Specialist > Legacy Laboratory Pathology > Legacy Central Lab > 1225 N.E. 2nd Ave. > Portland, OR > 972322003 > 503-944-7924 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at holml at umn.edu * From sandra.cheasty at wisc.edu Mon Apr 22 10:33:51 2019 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Mon, 22 Apr 2019 15:33:51 +0000 Subject: [Histonet] Leica RM2235 Block Clamp Lever snapped! Message-ID: Hello all, The block clamp lever of one of our Leica RM2235 microtomes snapped off. The broken end is still in the (threaded?) hole. Any advice or source for fixing/replacing this is most appreciated. Thanks, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From criley at dpspa.com Mon Apr 22 13:56:38 2019 From: criley at dpspa.com (Charles Riley) Date: Mon, 22 Apr 2019 14:56:38 -0400 Subject: [Histonet] Retic staining Message-ID: I am having trouble getting my retic stain to come out strong. It is working but Pathologists are saying they shouldn't have to go to high power just to see it (and I agree). I am using a brand new American MasterTech Chandler's Precision kit. I have followed all the steps correctly (one difficulty is getting the Ammonium hydroxide to go drop by drop so this may be the issue). I have tried staining longer in ammoniacal silver. more time in 20% formalin, more and less time in gold chloride and sodium thiosulfate. If anyone has any suggestions or uses this kit with time modifications please let me know. Willing to try anything to get it working. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From aperl at cmmedical.com Mon Apr 22 14:09:39 2019 From: aperl at cmmedical.com (Perl , Alison) Date: Mon, 22 Apr 2019 19:09:39 +0000 Subject: [Histonet] [EXTERNAL] Retic staining In-Reply-To: References: Message-ID: <590252c4857b412cba5915178d167f46@MK-EXMB02.mkmg.com> Hi Charles I've had very good luck in the past with Poly Scientific's Gomori's retic kit, which is ready-to-use (no ammoniacal preparation!) and very reliable. I haven't used AMT's. I wonder if anything changed in the transition to StatLab ownership? Making the ammoniacal generally seems to be the trickiest part... Alison Perl, HTL(ASCP)CM Anatomic Pathology Manager (914) 302-8424 aperl at cmmedical.com -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, April 22, 2019 2:57 PM To: Histo List Subject: [EXTERNAL] [Histonet] Retic staining I am having trouble getting my retic stain to come out strong. It is working but Pathologists are saying they shouldn't have to go to high power just to see it (and I agree). I am using a brand new American MasterTech Chandler's Precision kit. I have followed all the steps correctly (one difficulty is getting the Ammonium hydroxide to go drop by drop so this may be the issue). I have tried staining longer in ammoniacal silver. more time in 20% formalin, more and less time in gold chloride and sodium thiosulfate. If anyone has any suggestions or uses this kit with time modifications please let me know. Willing to try anything to get it working. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and its attachments, if any, may contain confidential or proprietary information and are intended solely for authorized use by the intended recipient(s) only. Any other use of this email is prohibited. If you have received this email in error, you are hereby notified that any retention, disclosure, copying, forwarding, distribution (in whole or in part and whether electronically, written and/or orally) and/or taking of any action in reliance on this email, its contents and/or any attachments thereto is strictly prohibited. If you received this email in error, please notify the sender by replying to this message and permanently delete this email, and any attachments thereto, from your system immediately. From Nilufa.Sultana at uts.edu.au Mon Apr 22 18:08:38 2019 From: Nilufa.Sultana at uts.edu.au (Nilufa Sultana) Date: Mon, 22 Apr 2019 23:08:38 +0000 Subject: [Histonet] Request for Feedback for Cryostats Message-ID: Hi all, We're looking at purchasing a new Cryostats for the teaching/research lab. Looking at Leica, Sakura and Thermo models. I'd love to hear any feedback on any model you might be using. Thank you for your kind cooperation. Kind regards Nilufa Sultana Nilufa.sultana at uts.edu.au UTS CRICOS Provider Code: 00099F DISCLAIMER: This email message and any accompanying attachments may contain confidential information. If you are not the intended recipient, do not read, use, disseminate, distribute or copy this message or attachments. If you have received this message in error, please notify the sender immediately and delete this message. Any views expressed in this message are those of the individual sender, except where the sender expressly, and with authority, states them to be the views of the University of Technology Sydney. Before opening any attachments, please check them for viruses and defects. Think. Green. Do. Please consider the environment before printing this email. From relia1 at earthlink.net Tue Apr 23 11:25:42 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 23 Apr 2019 12:25:42 -0400 Subject: [Histonet] Happy Lab Week!! Here are some fun ideas to celebrate!! Message-ID: <000001d4f9f1$32a47420$97ed5c60$@earthlink.net> Hello Histopeeps!! I hope you are doing well. I wanted to send a special bulletin wishing you a Happy National Medical Laboratory Professionals Week. I Hope Your Lab Has Been Having Some Fun, Informative & Special Events To Celebrate Because You Deserve It! I have heard of some really cool things that labs are doing to mark the occasion including luncheons, costume, trivia and photo contests and displays to educate others about what you do. If you get a chance check out the ASCP?s website section for Lab week. Here is the link: ?http://www.ascp.org/labweek I would love to hear what you are doing in your lab to celebrate so please shoot me back an e-mail and let me know. I also wanted to let you know about some exciting new positions I am working on. I Have Amazing Opportunities In WI, TX, VA, CA, NC, OH, AL and TN!!! Here are the positions!!! ? AP Manager ? Milwaukee, WI ? Histology Supervisor ? Dallas, TX ? Senior Histotechnologist ? Virginia Beach, VA ? Histology Territory Sales Manager ? Mid ?east region ? Histology Tech ? Greensboro, NC ? Histology Tech ? RTP ? NC ? Histology Tech ? Charlotte, NC ? Dermpath Histotech ? Birmingham, AL ? Histotechnician ? Modesto, CA ? Grossing Histotech ? Chattanooga, TN All of my positions are full time permanent positions my clients offer excellent compensation, benefits and relocation assistance and or sign on bonuses. If you or anyone you know might be interested please contact me. I can be reached toll free at 866-607-3542 or at relia1 at earthlink.net Remember if you refer someone to me and I place them you will earn a referral fee. Consider it fun money for your Summer Vacation!! Thanks-Pam #jobs4myhistopeeps Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From CDavis at che-east.org Tue Apr 23 12:56:29 2019 From: CDavis at che-east.org (Cassie P. Davis) Date: Tue, 23 Apr 2019 17:56:29 +0000 Subject: [Histonet] Retic staining Message-ID: <5c19a09c84f447a2bbc53704c9ffb3b2@che-east.org> Hi Charles, in addition to the drop size clean glassware makes a big difference. I used to use new urine cups to ensure the silver was being made in a clean vessel. I hope this helps Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Jose.R.deGuzman at gunet.georgetown.edu Tue Apr 23 13:05:59 2019 From: Jose.R.deGuzman at gunet.georgetown.edu (deGuzman, Jose R) Date: Tue, 23 Apr 2019 18:05:59 +0000 Subject: [Histonet] Retic staining Message-ID: Hi Charles, Ammoniacal Silver is a tricky step, we've had a lot of repeat Bielschowsky stains in the past due to it. You don't need to go drop by drop, what's key is to gently stir the solution as you slowly add ammonium hydroxide until the precipitate is reincorporated back into the solution. I don't know if your protocol has a specific amount of ammonium hydroxide to add but we've found that sometimes it takes less and sometimes it takes more than what is recommended to clear. Make sure you stir and keep an eye on the solution to make sure you only add what is needed for the reaction. Jose -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, April 23, 2019 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 185, Issue 14 I am having trouble getting my retic stain to come out strong. It is working but Pathologists are saying they shouldn't have to go to high power just to see it (and I agree). I am using a brand new American MasterTech Chandler's Precision kit. I have followed all the steps correctly (one difficulty is getting the Ammonium hydroxide to go drop by drop so this may be the issue). I have tried staining longer in ammoniacal silver. more time in 20% formalin, more and less time in gold chloride and sodium thiosulfate. If anyone has any suggestions or uses this kit with time modifications please let me know. Willing to try anything to get it working. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ---------------------------------------------------------------------- MedStar Health is a not-for-profit, integrated healthcare delivery system, the largest in Maryland and the Washington, D.C., region. Nationally recognized for clinical quality in heart, orthopaedics, cancer and GI. IMPORTANT: This e-mail (including any attachments) may contain information that is private, confidential, or protected by attorney-client or other privilege. If you received this e-mail in error, please delete it from your system without copying it and notify sender by reply e-mail, so that our records can be corrected... Thank you. Help conserve valuable resources - only print this email if necessary. From vollecra at gmail.com Tue Apr 23 14:27:22 2019 From: vollecra at gmail.com (Craig) Date: Tue, 23 Apr 2019 14:27:22 -0500 Subject: [Histonet] Suggestions on a used microtome Message-ID: Hi everyone! Starting a new lab Texas and was looking at getting a used microtome and wanted to see if any of you had suggestions on the best model to get used and where? Any suggestion will be greatly appreciated! Best, Craig From DSiena at statlab.com Tue Apr 23 15:10:28 2019 From: DSiena at statlab.com (Debra Siena) Date: Tue, 23 Apr 2019 20:10:28 +0000 Subject: [Histonet] Retic staining In-Reply-To: <5c19a09c84f447a2bbc53704c9ffb3b2@che-east.org> References: <5c19a09c84f447a2bbc53704c9ffb3b2@che-east.org> Message-ID: Hi all Also the freshness of the ammonium hydroxide can make a difference in how much you have to add to get the proper reactions. Fresher works better and faster. Also I use one of the plastic disposable properties to do drop by drop while swirling in s small acid cleaned flask or new plastic container. Debbie Siena Get Outlook for iOS ________________________________ From: Cassie P. Davis via Histonet Sent: Tuesday, April 23, 2019 1:07 PM To: histonet Subject: [Histonet] Retic staining *** Externally sourced email message *** Hi Charles, in addition to the drop size clean glassware makes a big difference. I used to use new urine cups to ensure the silver was being made in a clean vessel. I hope this helps Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7CDSiena%40statlab.com%7C842c89053d194854dd3508d6c816822d%7C39f23cf8abe54121b1662cb15967a2ec%7C0%7C0%7C636916396334167921&sdata=QzHUJpWTcWCx8DHc2Qkf6smrvTqwQt9YVHzPegdwVzE%3D&reserved=0 From wdesalvo.cac at outlook.com Tue Apr 23 15:19:13 2019 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Tue, 23 Apr 2019 20:19:13 +0000 Subject: [Histonet] Suggestions on a used microtome In-Reply-To: References: Message-ID: Contact Rankin for reliable refurbished instruments. I am partial to retracting microtones. Best to work with a company that provides quality William DeSalvo ________________________________ From: Craig via Histonet Sent: Tuesday, April 23, 2019 12:27:22 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Suggestions on a used microtome Hi everyone! Starting a new lab Texas and was looking at getting a used microtome and wanted to see if any of you had suggestions on the best model to get used and where? Any suggestion will be greatly appreciated! Best, Craig _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bonnie_salyer at tedpella.com Wed Apr 24 15:18:25 2019 From: bonnie_salyer at tedpella.com (Bonnie Salyer) Date: Wed, 24 Apr 2019 13:18:25 -0700 Subject: [Histonet] Job Opportunity - Ted Pella, Inc. Message-ID: <00d301d4fada$dfd6fc50$9f84f4f0$@tedpella.com> Ted Pella, Inc. has been a worldwide leader in the microscopy supplies and specimen preparation instrumentation field for over 50 years. We are seeking qualified applicants to join our team as a Life Science Product Specialist. This is an exciting opportunity to be involved in product development and growth of our TEM and LM life sciences-related product lines. Ideal candidates should have BS in Life Sciences or related, minimum of 5 years hands-on experience with both TEM and LM instrument use and specimen preparation, HTL certification is a plus, Immunohistochemistry (IHC) is a plus. This position is located in beautiful Redding, CA - live and work in an area surrounded by some of Northern California's most beautiful natural attractions, and in the midst of small city cultural entertainment, history, dining and shopping. No more long commutes to work, and only minutes away from amazing waterfall hikes, cycling trails and nationally-ranked fishing. See our complete job posting on our website at www.tedpella.com Bonnie Salyer, SPHR, SHRM-SCP Human Resources Manager Ted Pella, Inc. Phone: 530-243-2200 x260 Fax: 530-243-8278 bonnie_salyer at tedpella.com www.tedpella.com From POWELL_SA at mercer.edu Thu Apr 25 07:48:30 2019 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Thu, 25 Apr 2019 12:48:30 +0000 Subject: [Histonet] The Georgia Society For Histotechnology 2019 Histopalooza meeting In-Reply-To: References: Message-ID: My First message was too large, here is another try. Shirley Hi Everyone, The Georgia Society for Histotechnology has a new website. Visit the site here: http://georgiahistotech.org/gsh-symposium/ You may download the registration forms to print and mail with a check or pay through PayPal with credit card. The hotel reservation information is on the same page. Come to Georgia coast for a great meeting. There is only a week and a half left, so make plans today. The program at a glance is below to make your choices of workshops. Shirley Powell GSH Registrar Georgia Society for Histotechnology HISTOPALOOZA! May 3-5, 2019 PROGRAM Hotel Tybee, Tybee Island, Ga Friday ROOM A 8:30 am -5:00 pm ROOM B 3:30 pm -4:30 pm #1: A Guide to passing the HT/HTL Certification #2: The Cost of Reprocessing Carl Sagasser, MBA, BS, HT (ASCP) 6 hours Skip Brown, HT (ASCP) - 1 hour BREAK: 10:00-10:30 / LUNCH: 12:00 - 1:30 / Break: 3:00 - 3:30 VENDOR RECEPTION: 7-10 p.m. in Vendor Exhibit Hall Saturday ROOM A 8:30 am-12:00 am ROOM B 8:30 am- 9:30 am #3 Leadership:The Pinnacle of Management & #4: The benefits of automation or why to Supervision - Skip Brown, HT(ASCP)- 3 hours move away from dip and dunk systems Dan Hopkins, Roche - 1 hour BREAK: 10:00-10:30 in Vendor Exhibit Hall ROOM B 10:30 am- 11:30 am #5 Equipment Maintenance Matt Lanford, TechOne- 1 hour AWARDS LUNCHEON - 12:00-1:30 ROOM A 1:30 pm- 5:00 pm ROOM B 1:30 pm - 3:00 pm #6 Introduction to IHC #7 How to Prepare Macro and Not So Kimya Jones HT, QIHC (ASCP)- 3 hours Macro Sections Shirley Powell, HTL, HT(ASCP)- 1.5 hours BREAK: 3:00-3:30 in Vendor - in the Exhibit Hall ROOM B 3:30 pm- 5:00 pm #8 Taking Control of Your IHC Controls Steve Westra, StatLab- 1.5 hours General GSH Membership Meeting at 5:00 - ROOM B Sunday Room A 8:30 am- 12:00 pm ROOM B 8:30 am- 9:30 am #9: Human Tissues: Histological Identification of #10 Digital Pathology: What it is, how and the Major Tissue Types -- where it is used, and why you should Ely Klar, MS, HTL (ASCP) - 3 hours care Aigars Brandts, PhD, Roche- 1 hour BREAK: 10:00-10:30 in Vendor Exhibit Hall NOTE: Vendor exhibits close at noon From LRaff at uropartners.com Thu Apr 25 08:20:36 2019 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 25 Apr 2019 13:20:36 +0000 Subject: [Histonet] Bar code scanner to compare specimen jars to cassettes Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF121EE80A@COLOEXCH01.uropartners.local> Greetings to all: Are any of your labs using a portable device (not part of an LIS) to compare bar codes on cassettes to 1) specimen jars, or 2) slides. If so, what product do you use, how does it affect workflow, and how satisfied are you with it? Thanks, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From Nancy_Schmitt at pa-ucl.com Thu Apr 25 12:21:11 2019 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Thu, 25 Apr 2019 17:21:11 +0000 Subject: [Histonet] Artisan AFB contamination Message-ID: <4cb84698308143b693565632645b5a73@pa-ucl.com> Hello- Has anyone had any issues with AFB contamination on the Artisan special stainer? We are doing a monthly process with alcohol and drying of the containers and flushing the lines with alcohol per Technical support at Agilent - still seeing it pop up occasionally. Appreciate any thoughts- Nancy Schmitt Untied Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From andy at vabderm.com Thu Apr 25 13:20:23 2019 From: andy at vabderm.com (andy at vabderm.com) Date: Thu, 25 Apr 2019 12:20:23 -0600 Subject: [Histonet] Bot Fly larva processing Message-ID: <000401d4fb93$8d1820c0$a7486240$@vabderm.com> Hey Histonet, Does anyone have a good processing program for bot fly larva? Also looking for grossing recommendations. My guess is submit cross sections. Any hints would be greatly appreciated. Andy Fortune Histology Manager Glenwood Dermatology Glenwood Springs, CO From Valerie.Hannen at parrishmed.com Fri Apr 26 05:16:13 2019 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Fri, 26 Apr 2019 06:16:13 -0400 Subject: [Histonet] Physcian office urine Message-ID: <450B7A81EDA0C54E97C53D60F00776C3245EC06FB5@isexstore03> Good morning, I am hoping to get your opinion on this. Do you send Saccommano fluid to the doctor's offices for them to put into there urine for cytology specimens or just have them refrigerate the specimens until delivered to the Lab? My Lab Manager is asking which is best practice, we both have our opinions, but they don't match. Happy Lab Week everyone!! Thanks so much, Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From BMolinari at texasheart.org Fri Apr 26 07:21:48 2019 From: BMolinari at texasheart.org (Betsy Molinari) Date: Fri, 26 Apr 2019 12:21:48 +0000 Subject: [Histonet] biopsy staining References: <8bce4a42-9403-415d-9ef0-7b193d29f473.a2f9f702-9a57-4a72-ac71-a5856150e07c.ea79c371-1ada-48ac-b13f-932d2b5a9f34@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.32884165-05c9-44b8-b118-3d355c594a17@emailsignatures365.codetwo.com> Message-ID: Hi, I have researched the archives but still have concerns. I have some very small pieces of tissue that are in 70% ETOH. Should I mark them with eosin or safranin O? From what I have read the tissues were still in formalin, and mine are already in alcohol. Can they still be marked? At the moment they are not planning to do florescence, but I would like to keep that option open. Thanks, Betsy Molinari, HT (ASCP) Texas Heart Institute 6770 Bertner Ave Houston, TX 77030 832-355-6524 (lab) 832-355-6812 (fax) Betsy Molinari Sr. Histology Research Technician CV Pathology Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From BMolinari at texasheart.org Fri Apr 26 10:26:23 2019 From: BMolinari at texasheart.org (Betsy Molinari) Date: Fri, 26 Apr 2019 15:26:23 +0000 Subject: [Histonet] biopsy staining In-Reply-To: References: <8bce4a42-9403-415d-9ef0-7b193d29f473.a2f9f702-9a57-4a72-ac71-a5856150e07c.ea79c371-1ada-48ac-b13f-932d2b5a9f34@emailsignatures365.codetwo.com> <8bce4a42-9403-415d-9ef0-7b193d29f473.0d46ef2e-c77a-43a2-9e67-5fe2fe9ca513.32884165-05c9-44b8-b118-3d355c594a17@emailsignatures365.codetwo.com> Message-ID: I will try the safranin O on two samples. Also, toluidine blue has been suggested. Do you have any experience with it? Betsy Molinari Sr. Histology Research Technician CV Pathology Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: BMolinari at texasheart.org texasheart.org | facebook | twitter -----Original Message----- From: Lynette Pavelich [mailto:lynettepav at gmail.com] Sent: Friday, April 26, 2019 9:20 AM To: Betsy Molinari Subject: Re: [Histonet] biopsy staining *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. ________________________________ I can?t think of a reason why it wouldn?t work or hurt. Give it a try! Dab off the alcohol and mark. Let us know how it worked out in the end! > On Apr 26, 2019, at 10:04 AM, Betsy Molinari wrote: > > That was my concern as well. Since the tissue is already in 70% ETOH can it still be marked? > > > Betsy Molinari > Sr. Histology Research Technician > CV Pathology > > Texas Heart Institute > 6770 Bertner Avenue, MC 1-283 > Houston, TX 77030 > > Office: 832-355-6524 | Fax: 832-355-6812 > Email: BMolinari at texasheart.org > texasheart.org | facebook | twitter > -----Original Message----- > From: Lynette Pavelich [mailto:lynettepav at gmail.com] > Sent: Friday, April 26, 2019 7:55 AM > To: Betsy Molinari > Subject: Re: [Histonet] biopsy staining > > *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. > ________________________________ > > > I would not choose eosin then because of its fluorescent properties. > > Lynette Pavelich, HT(ASCP), QIHC > > Sent from my iPhone > >> On Apr 26, 2019, at 8:21 AM, Betsy Molinari via Histonet wrote: >> >> Hi, >> I have researched the archives but still have concerns. I have some very small pieces of tissue that are in 70% ETOH. Should I mark them with eosin or safranin O? From what I have read the tissues were still in formalin, and mine are already in alcohol. Can they still be marked? At the moment they are not planning to do florescence, but I would like to keep that option open. >> Thanks, >> Betsy Molinari, HT (ASCP) >> Texas Heart Institute >> 6770 Bertner Ave >> Houston, TX 77030 >> 832-355-6524 (lab) >> 832-355-6812 (fax) >> >> Betsy Molinari >> Sr. Histology Research Technician >> CV Pathology >> >> Texas Heart Institute >> 6770 Bertner Avenue, MC 1-283 >> Houston, TX 77030 >> >> Office: 832-355-6524 | Fax: 832-355-6812 >> Email: BMolinari at texasheart.org >> texasheart.org | facebook | twitter >> >> This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIFAg&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=cHhb45byVhOxSFbivksM2zWEfl4MWs5JTZK6OYKxZlU&s=K9RNgsjqcWmyx9OrsTyV8PuXkc0lvho75ejDhZMUfAo&e= From john.garratt at ciqc.ca Fri Apr 26 10:44:21 2019 From: john.garratt at ciqc.ca (John Garratt) Date: Fri, 26 Apr 2019 15:44:21 +0000 Subject: [Histonet] Physcian office urine In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C3245EC06FB5@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C3245EC06FB5@isexstore03> Message-ID: If you are referring to "screening urines" and not diagnostic (ie cystoscopy specimens) my personal preference would be to get a series of three early morning specimens which have been kept refrigerated and delivered on ice. The urine was already slopping around the warm bladder for hours anyway. If there was anything atypical in the screening urine then get a better preserved specimen using your diagnostic urine protocol that would utilize a fixative like Cytolyt or whatever your preference. John www.ciqc.ca ??????? Original Message ??????? On Friday, April 26, 2019 3:16 AM, Hannen, Valerie via Histonet wrote: > Good morning, I am hoping to get your opinion on this. Do you send Saccommano fluid to the doctor's offices for them to put into there urine for cytology specimens or just have them refrigerate the specimens until delivered to the Lab? My Lab Manager is asking which is best practice, we both have our opinions, but they don't match. > > Happy Lab Week everyone!! > > Thanks so much, > > Valerie Hannen,MLT(ASCP),HTL,SU (FL) > Section Chief, Histology > Parrish Medical Center > 951 N. Washington Ave. > Titusville,Florida 32796 > T: (321)268-6333 ext. 7506 > F: (321) 268-6149 > valerie.hannen at parrishmed.commailto:valerie.hannen at parrishmed.com > > www.parrishmed.com > > =================== > > "This email is intended solely for the use of the individual to > whom it is addressed and may contain information that is > privileged, confidential or otherwise exempt from disclosure > under applicable law. If the reader of this email is not the > intended recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you > have received this communication in error, please immediately > delete this message. Thank you" > > ============================================================================================================================================================================================================================================================================================================================================================================================================================================================================================================================================================================================= > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Fri Apr 26 13:39:54 2019 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 26 Apr 2019 14:39:54 -0400 Subject: [Histonet] biopsy staining In-Reply-To: References: Message-ID: Betsy Molinari, HT (ASCP) at the Texas Heart Institute in Houston asks: >>I have some very small pieces of tissue that are in 70% ethanol. Should I mark them with eosin or safranin O? From what I have read the tissues were still in formalin, and mine are already in alcohol. Can they still be marked? At the moment they are not planning to do fluorescence, but I would like to keep that option open.<< I've excellent results with safranin O, which (unlike eosin) is not fluorescent. The solution used for Gram staining by bacteriologists serves well. The alcohol storage shouldn't matter, but try it. Bob Richmond Samurai Pathologist Maryville TN From carl.hobbs at kcl.ac.uk Sat Apr 27 12:30:48 2019 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sat, 27 Apr 2019 17:30:48 +0000 Subject: [Histonet] biopsy staining Message-ID: Hi Are you processing to Pwax? If so, I stain my mouse DRGS ( very small) using Haemalum. 5 mins max ( I use Gill's I) then blue in alkaline tapwater for 10 mins before Pwax processing Stain from Formalin or alcohol but.....rinse in dist water to remove before staining. The "blue" stays nicely in the tissues, for embedding : sure, I then subject the sections to HIER without pretreatment as the citric acid treatment gets rid of any dye. If doing dye-staining I will acid-alcohol treat the sections before staining. Works well for me. Good luck Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From barry_m600 at optusnet.com.au Sun Apr 28 16:52:14 2019 From: barry_m600 at optusnet.com.au (barry) Date: Mon, 29 Apr 2019 07:52:14 +1000 Subject: [Histonet] Leica Bond 3 Message-ID: <000501d4fe0c$a5102b70$ef308250$@optusnet.com.au> Hi, Is anyone experiencing problems with the Bond 3 immunostainer software version 6.0.0.365 and the new Leica labels for the BOND. We have 3 BONDS in our department and have used the BOND platform for over 10 years. Previous software versions have been much more user friendly. Regards Barry Madigan Pathology Queensland From relia1 at earthlink.net Tue Apr 30 09:38:19 2019 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 30 Apr 2019 10:38:19 -0400 Subject: [Histonet] YOU are in The Catbird Seat!! Message-ID: <000001d4ff62$5aea7620$10bf6260$@earthlink.net> Hi Histopeeps, How are you? I hope you are having a great week! Last year I sent you an email telling you that about the old saying ?In the Catbird Seat? It means you are calling the shots. That?s right YOU! That?s what has been developing over the past few years in the histology field and it?s getting BETTER and BETTER!!!!! ? If you are an ASCP certified histotech with at least 2 years of experience ? If you are a recent graduate of a histology program who is certified or eligible ? If you are an experienced tech with more than 2 years of experience not certified but eligible and willing to get certified EVERY ONE OF YOU is in the CATBIRD SEAT!!!!! Things have come full cycle and you are in GREAT DEMAND. There are many more histology jobs than histotechs. There are sign on bonuses, leading edge training opportunities and amazing perks waiting for you!! Histopeeps, if you are considering a job change: ? Because you want to relocate ? Because you feel unchallenged ? Because you want more money, a better shift, nicer benefits Whatever the reason? **STRIKE WHILE THE IRON?S HOT!! Shoot me a quick email and let me know what you would like to do and where you would like to go and when (no pressure the timing is up to YOU!) I will keep you posted on opportunities that match your interests. **REMEMBER IT NEVER HURTS TO LOOK!! I am including a list of my current opportunities in case one might strike your fancy. Please feel free to pass the info along to your friends and coworkers. If I place someone you refer to me you will earn a referral reward! RELIA?S Current Histology Opportunities: ? IHC Specialist ? Los Angeles - based (travel required) ? IHC Specialist ? Phoenix, AZ ?based (travel required) ? Histotech ?Greensboro, NC ? day or night shift ? your choice! ? Histotech ? Annapolis, MD Days! ? Histology Territory Sales ? OH based (travel required) ? Histology Territory Sales ? New England territory (travel required) ? Gross Room Supervisor ? Norfolk, VA Days!! ? Histotech ? Nashville ? Grossing Histotech ? Chattanooga, TN ? Histotech ? Milwaukee ? Days SIGN ON BONUS!! ? Histotechnician ? Modesto, CA ? Histology Tech ? Soldotna, AK ? Histotech ? Charlotte, NC ? Nights ? Histotech ? NYC NY lic required All of my clients offer excellent compensation, benefits and most offer relocation assistance and or sign on bonuses. All of these jobs are full time & permanent & most of them are RELIA Exclusives!!! I can be reached ASAP via email at relia1 at earthlink.net or toll free at the office at 866-607-3542 or on my cell at 407-353-5070 call or text! Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From tpalmer at wallisderm.com Tue Apr 30 15:29:50 2019 From: tpalmer at wallisderm.com (Tracie Palmer) Date: Tue, 30 Apr 2019 15:29:50 -0500 Subject: [Histonet] Average cost to perform a 88304 or 88305 Message-ID: I was wanting to see if someone can share what your average cost is to prepare slides for a 88304 or a 88305 with no specials just a routine H&E. Thank you, Tracie Palmer, HT (ASCP) Laboratory Supervisor Wallis Dermatology Associate Longview, TX -- Tracie Palmer, HT (ASCP) Laboratory Supervisor Wallis Dermatology Associates 4001 Technology Center Suite 304 Longview, Texas 75605 Lab 903-918-8004 Fax 903-663-2454 Cell 903-235-9269 From Dawn.Olszewski at SGMC.ORG Tue Apr 30 16:05:44 2019 From: Dawn.Olszewski at SGMC.ORG (Olszewski, Dawn) Date: Tue, 30 Apr 2019 21:05:44 +0000 Subject: [Histonet] Job Descriptions Message-ID: Hello Histo Peeps, My director has asked me to help her develop new job descriptions in the hopes of retaining histotechs by giving them a career ladder. She would like to have 3 levels for HT's and 3 levels for HTL's. If anyone has any suggestions, please reply or shoot me a personal e-mail. I would greatly appreciate the help! Thanks so much! Dawn Olszewski HTL(ASCP)QIHC Pathology Manager South Georgia Medical Center P: (229) 259-4830 E: dawn.olszewski at sgmc.org