From julio.benavides at csic.es Mon Sep 3 02:58:02 2018 From: julio.benavides at csic.es (=?UTF-8?Q?Julio_Benavides_Silv=c3=a1n?=) Date: Mon, 3 Sep 2018 09:58:02 +0200 Subject: [Histonet] Grossing room design Message-ID: Hi there, we are in the process of getting a new grossing room and I was wondering if there is any document or similar where the basic design requirements are stated. Something like the ventilation, material of the walls, requirements for electricity standards, pipes..... Any suggestions/ideas/experience would be much appreciated! Thanks a lot for your help!! Julio -- Julio Benavides Silv?n Instituto de Ganader?a de Monta?a (CSIC-Universidad de Le?n) 24346. Grulleros, Le?n +34 987317156 ext.861162 From Richard.Cartun at hhchealth.org Mon Sep 3 08:42:58 2018 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Mon, 3 Sep 2018 13:42:58 +0000 Subject: [Histonet] Unstained slides In-Reply-To: <1153390626.9774240.1534702149282@mail.yahoo.com> References: <1153390626.9774240.1534702149282@mail.yahoo.com> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EA8A81484@HHCEXCHMB03.hhcsystem.org> It appears that the presence of water, both endogenously and exogenously, plays a central role in the loss of antigenicity in stored unstained slides (see reference below). Labs that are experiencing significant loss of immunoreactivity in their unstained slides should check their tissue processing. Xie R, Chung J-Y, Ylaya K, et al.: Factors influencing the degradation of archival formalin-fixed, paraffin-embedded tissue sections. J of Histochem Cytochem 2011; 59:356-365. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Sunday, August 19, 2018 2:09 PM To: Frazier, John; Terri Braud Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Unstained slides This is an email from Outside HHC. USE CAUTION opening attachments or links from unknown senders. Everything has been pointed out is correct BUT also pivot on "how the unstained slides are kept".Kept in a box their "useful life" is quite short (not beyond 1 week at the most).Kept at -80?C I have used them after years of being stored the principle being of deep-freezing and this is "standard procedure" for IDF "+ controls".Kept in a Coplin jar filled with mineral oil or paraffin covered I have used them after months of being stored the principle being that, isolated from air oxygen, epitopes do not oxidize ("weaken") of if they do, the rate is greatly slowed.On the other hand, usually, unstained slides are kept for only few days in the event that, lets say within a week, the PT decides to order some special procedure and sometimes it is impossible "return" to the original block many times "almost exhausted".Properly done storing unstained slides are extremely useful.Ren? On Sunday, August 19, 2018 1:52 PM, "Frazier, John via Histonet" wrote: I agree with Tim as well. This is what we advise our clients to do. It takes some coordination with the pathologist, but it is the best strategy for reducing unnecessary unstained slides. In the studies that we have performed only 10% of the unstained slides that are cut are you and the 90% are are it takes some coordination with the pathologist, but it is the best strategy for reducing unnecessary unstained slides. In the studies that we have performed only 10% of the unstained slides that are cut are you and 90% are thrown away thrown away. Several laboratories that I have visited in order to reduce the amount of wasted tissue when refacing the blocks, is to reseal the blocks with liquid paraffin, that have scant or small amounts of tissue in the block, such as the needle core biopsy. Bottom line on this issue is to educate the pathologist, and not water and stain slides except in rare occasions Sent from my iPhone > On Aug 17, 2018, at 14:07, Terri Braud wrote: > > I'm with Tim Morken on this one. The variability of antigenicity in storage is so wide open, and there really is no recent data, so we just make a point of educating our techs on not wasting tissue/levels during sectioning. If the techs feel that the residual tissue in the block is in danger of being exhausted, we communicate with our pathologists on how best to handle any requests. Unstained slides was time, money, and storage and we are better off without them. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Today's Topics: > 7. Re: Unstained slides - how long are they good for? > (Morken, Timothy) > > Message: 7 > Date: Fri, 17 Aug 2018 15:16:00 +0000 > From: "Morken, Timothy" > To: P Sicurello > Subject: Re: [Histonet] Unstained slides - how long are they good for? > > > Paula, since it is variable we strive to not have unstained slides. We had kept them indefinitely, then when storage was overwhelming us we reduced it to 2 months maximum. Now we require request for unstained to be ordered in the system and delivered to the pathologist. We do not hold any in the lab. We recut when new stains are ordered. In the past we had routinely cut extras "just in case" but ended up with thousands of unstained slides that were never used. Instead we trained everyone to reduce wastage and get good sections from a cut block with minimal facing. We have not stored unstained sections for many years and they do not seem to be missed. > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology UC San Francisco Medical Center > > > -----Original Message----- > From: P Sicurello via Histonet > [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, August 16, 2018 4:49 PM > To: HistoNet > Subject: [Histonet] Unstained slides - how long are they good for? > > Hello My Fellow Histologists, > > Happy Friday Eve. > > The question has come up...... How long are *unstained* slides good for? > Not for H&E but tests like IHC and molecular testing. These slides > have been cut, stored at room temperature, not sealed in anyway, and > kept in a cardboard box. > > Please let me know what your opinions are and what your retention > policy is concerning *unstained* slides. > > Thanks oodles. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail > is intended only for the person or entity to which it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action > in reliance upon this information by persons or entities other than > the intended recipient is prohibited. If you received this e-mail in > error, please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVv > RFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=Di_I > rEz2lxTIgibc_SPWiFaTGmnw4SYfIOvpPRy-TDM&s=4L8HS7D7SpEHBR1sDxuVWj8zyMgT > _QNsAkdyu2fu2P4&e= > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVv > RFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=Di_I > rEz2lxTIgibc_SPWiFaTGmnw4SYfIOvpPRy-TDM&s=4L8HS7D7SpEHBR1sDxuVWj8zyMgT > _QNsAkdyu2fu2P4&e= > > ------------------------------ > > End of Histonet Digest, Vol 177, Issue 16 > ***************************************** > > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=Di_IrEz2lxTIgibc_SPWiFaTGmnw4SYfIOvpPRy-TDM&s=4L8HS7D7SpEHBR1sDxuVWj8zyMgT_QNsAkdyu2fu2P4&e= | | Virus-free. | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.avast.com&d=Dw | IGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPkn | IMjMlCkk-KGKHflslRsFz3l5JE&m=Di_IrEz2lxTIgibc_SPWiFaTGmnw4SYfIOvpPRy-T | DM&s=QSBL3lVp0DYppOhOIx51poBgB1vYHNFN4lWV9DhKvGc&e= | _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=Di_IrEz2lxTIgibc_SPWiFaTGmnw4SYfIOvpPRy-TDM&s=4L8HS7D7SpEHBR1sDxuVWj8zyMgT_QNsAkdyu2fu2P4&e= Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From john.frazier at roche.com Mon Sep 3 10:11:10 2018 From: john.frazier at roche.com (Frazier, John) Date: Mon, 3 Sep 2018 11:11:10 -0400 Subject: [Histonet] Unstained slides In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2EA8A81484@HHCEXCHMB03.hhcsystem.org> References: <1153390626.9774240.1534702149282@mail.yahoo.com> <9215BD4B0BA1B44D962A71C758B68D2EA8A81484@HHCEXCHMB03.hhcsystem.org> Message-ID: Interesting that you stated that, I was at the university of Colorado this past week and was speaking with the medical director of the pathology department. We actually started talking about unstained slides and their storage conditions. We actually spoke of the histonet discussions around unstained slide storage. He stated to me that due to the elevation and lack of humidity in Denver that the antigenicity of unstained slides has been up to multiple years. This is due to, as you stated, water in the tissue. Sent from my iPad > On Sep 3, 2018, at 9:42 AM, Cartun, Richard wrote: > > It appears that the presence of water, both endogenously and exogenously, plays a central role in the loss of antigenicity in stored unstained slides (see reference below). Labs that are experiencing significant loss of immunoreactivity in their unstained slides should check their tissue processing. > > Xie R, Chung J-Y, Ylaya K, et al.: Factors influencing the degradation of archival formalin-fixed, paraffin-embedded tissue sections. J of Histochem Cytochem 2011; 59:356-365. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 > (860) 545-2204 Fax > > -----Original Message----- > From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Sunday, August 19, 2018 2:09 PM > To: Frazier, John; Terri Braud > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Unstained slides > > This is an email from Outside HHC. USE CAUTION opening attachments or links from unknown senders. > > Everything has been pointed out is correct BUT also pivot on "how the unstained slides are kept".Kept in a box their "useful life" is quite short (not beyond 1 week at the most).Kept at -80?C I have used them after years of being stored the principle being of deep-freezing and this is "standard procedure" for IDF "+ controls".Kept in a Coplin jar filled with mineral oil or paraffin covered I have used them after months of being stored the principle being that, isolated from air oxygen, epitopes do not oxidize ("weaken") of if they do, the rate is greatly slowed.On the other hand, usually, unstained slides are kept for only few days in the event that, lets say within a week, the PT decides to order some special procedure and sometimes it is impossible "return" to the original block many times "almost exhausted".Properly done storing unstained slides are extremely useful.Ren? > > On Sunday, August 19, 2018 1:52 PM, "Frazier, John via Histonet" wrote: > > > I agree with Tim as well. This is what we advise our clients to do. It takes some coordination with the pathologist, but it is the best strategy for reducing unnecessary unstained slides. In the studies that we have performed only 10% of the unstained slides that are cut are you and the 90% are are it takes some coordination with the pathologist, but it is the best strategy for reducing unnecessary unstained slides. In the studies that we have performed only 10% of the unstained slides that are cut are you and 90% are thrown away thrown away. > Several laboratories that I have visited in order to reduce the amount of wasted tissue when refacing the blocks, is to reseal the blocks with liquid paraffin, that have scant or small amounts of tissue in the block, such as the needle core biopsy. > Bottom line on this issue is to educate the pathologist, and not water and stain slides except in rare occasions > > Sent from my iPhone > >> On Aug 17, 2018, at 14:07, Terri Braud wrote: >> >> I'm with Tim Morken on this one. The variability of antigenicity in storage is so wide open, and there really is no recent data, so we just make a point of educating our techs on not wasting tissue/levels during sectioning. If the techs feel that the residual tissue in the block is in danger of being exhausted, we communicate with our pathologists on how best to handle any requests. Unstained slides was time, money, and storage and we are better off without them. >> >> Terri L. Braud, HT(ASCP) >> Anatomic Pathology Supervisor >> Laboratory >> Holy Redeemer Hospital >> 1648 Huntingdon Pike >> Meadowbrook, PA 19046 >> ph: 215-938-3689 >> fax: 215-938-3874 >> Care, Comfort, and Heal >> >> Today's Topics: >> 7. Re: Unstained slides - how long are they good for? >> (Morken, Timothy) >> >> Message: 7 >> Date: Fri, 17 Aug 2018 15:16:00 +0000 >> From: "Morken, Timothy" >> To: P Sicurello >> Subject: Re: [Histonet] Unstained slides - how long are they good for? >> >> >> Paula, since it is variable we strive to not have unstained slides. We had kept them indefinitely, then when storage was overwhelming us we reduced it to 2 months maximum. Now we require request for unstained to be ordered in the system and delivered to the pathologist. We do not hold any in the lab. We recut when new stains are ordered. In the past we had routinely cut extras "just in case" but ended up with thousands of unstained slides that were never used. Instead we trained everyone to reduce wastage and get good sections from a cut block with minimal facing. We have not stored unstained sections for many years and they do not seem to be missed. >> >> Tim Morken >> Pathology Site Manager, Parnassus >> Supervisor, Electron Microscopy/Neuromuscular Special Studies >> Department of Pathology UC San Francisco Medical Center >> >> >> -----Original Message----- >> From: P Sicurello via Histonet >> [mailto:histonet at lists.utsouthwestern.edu] >> Sent: Thursday, August 16, 2018 4:49 PM >> To: HistoNet >> Subject: [Histonet] Unstained slides - how long are they good for? >> >> Hello My Fellow Histologists, >> >> Happy Friday Eve. >> >> The question has come up...... How long are *unstained* slides good for? >> Not for H&E but tests like IHC and molecular testing. These slides >> have been cut, stored at room temperature, not sealed in anyway, and >> kept in a cardboard box. >> >> Please let me know what your opinions are and what your retention >> policy is concerning *unstained* slides. >> >> Thanks oodles. >> >> Sincerely, >> >> Paula Sicurello, HTL (ASCP)CM >> >> Histotechnology Specialist >> >> UC San Diego Health >> >> 200 Arbor Drive >> >> San Diego, CA 92103 >> >> (P): 619-543-2872 >> >> >> >> *Confidentiality Notice*: The information transmitted in this e-mail >> is intended only for the person or entity to which it is addressed and >> may contain confidential and/or privileged material. Any review, >> retransmission, dissemination or other use of or taking of any action >> in reliance upon this information by persons or entities other than >> the intended recipient is prohibited. If you received this e-mail in >> error, please contact the sender and delete the material from any computer. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste >> rn.edu_mailman_listinfo_histonet&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVv >> RFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=Di_I >> rEz2lxTIgibc_SPWiFaTGmnw4SYfIOvpPRy-TDM&s=4L8HS7D7SpEHBR1sDxuVWj8zyMgT >> _QNsAkdyu2fu2P4&e= >> >> >> >> ------------------------------ >> >> Subject: Digest Footer >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste >> rn.edu_mailman_listinfo_histonet&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVv >> RFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=Di_I >> rEz2lxTIgibc_SPWiFaTGmnw4SYfIOvpPRy-TDM&s=4L8HS7D7SpEHBR1sDxuVWj8zyMgT >> _QNsAkdyu2fu2P4&e= >> >> ------------------------------ >> >> End of Histonet Digest, Vol 177, Issue 16 >> ***************************************** >> >> >> > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=Di_IrEz2lxTIgibc_SPWiFaTGmnw4SYfIOvpPRy-TDM&s=4L8HS7D7SpEHBR1sDxuVWj8zyMgT_QNsAkdyu2fu2P4&e= > > > > > | | Virus-free. > | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.avast.com&d=Dw > | IGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPkn > | IMjMlCkk-KGKHflslRsFz3l5JE&m=Di_IrEz2lxTIgibc_SPWiFaTGmnw4SYfIOvpPRy-T > | DM&s=QSBL3lVp0DYppOhOIx51poBgB1vYHNFN4lWV9DhKvGc&e= | > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=Di_IrEz2lxTIgibc_SPWiFaTGmnw4SYfIOvpPRy-TDM&s=4L8HS7D7SpEHBR1sDxuVWj8zyMgT_QNsAkdyu2fu2P4&e= > > > Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From rjbuesa at yahoo.com Mon Sep 3 10:23:12 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 3 Sep 2018 15:23:12 +0000 (UTC) Subject: [Histonet] Unstained slides In-Reply-To: References: <1153390626.9774240.1534702149282@mail.yahoo.com> <9215BD4B0BA1B44D962A71C758B68D2EA8A81484@HHCEXCHMB03.hhcsystem.org> Message-ID: <1789233342.347461.1535988192543@mail.yahoo.com> On this issue of lost of antigenicity, never forget air oxygen!Ren? On Monday, September 3, 2018 11:11 AM, "Frazier, John" wrote: Interesting that you stated that, I was at the university of Colorado this past week and was speaking with the medical director of the pathology department. We actually started talking about unstained slides and their storage conditions. We actually spoke of the histonet discussions around unstained slide storage.? He stated to me that due to the elevation and lack of humidity in Denver that the antigenicity of unstained slides has been up to multiple years. This is due to, as you stated, water in the tissue. Sent from my iPad > On Sep 3, 2018, at 9:42 AM, Cartun, Richard wrote: > > It appears that the presence of water, both endogenously and exogenously, plays a central role in the loss of antigenicity in stored unstained slides (see reference below).? Labs that are experiencing significant loss of immunoreactivity in their unstained slides should check their tissue processing. > > Xie R, Chung J-Y, Ylaya K, et al.:? Factors influencing the degradation of archival formalin-fixed, paraffin-embedded tissue sections.? J of Histochem Cytochem 2011; 59:356-365. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT? 06102 > (860) 972-1596 > (860) 545-2204 Fax > > -----Original Message----- > From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Sunday, August 19, 2018 2:09 PM > To: Frazier, John; Terri Braud > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Unstained slides > > This is an email from Outside HHC. USE CAUTION opening attachments or links from unknown senders. > > Everything has been pointed out is correct BUT also pivot on "how the unstained slides are kept".Kept in a box their "useful life" is quite short (not beyond 1 week at the most).Kept at -80?C I have used them after years of being stored the principle being of deep-freezing and this is "standard procedure" for IDF "+ controls".Kept in a Coplin jar filled with mineral oil or paraffin covered I have used them after months of being stored the principle being that, isolated from air oxygen, epitopes do not oxidize ("weaken") of if they do, the rate is greatly slowed.On the other hand, usually, unstained slides are kept for only few days in the event that, lets say within a week, the PT decides to order some special procedure and sometimes it is impossible "return" to the original block many times "almost exhausted".Properly done storing unstained slides are extremely useful.Ren? > >? ? On Sunday, August 19, 2018 1:52 PM, "Frazier, John via Histonet" wrote: > > > I agree with Tim as well. This is what we advise our clients to do. It takes some coordination with the pathologist, but it is the best strategy for reducing unnecessary unstained slides. In the studies that we have performed only 10% of the unstained slides that are cut are you and the 90% are are it takes some coordination with the pathologist, but it is the best strategy for reducing unnecessary unstained slides. In the studies that we have performed only 10% of the unstained slides that are cut are you and 90% are thrown away thrown away. > Several laboratories that I have visited in order to reduce the amount of wasted tissue when refacing the blocks, is to reseal the blocks with liquid paraffin, that have scant or small amounts of tissue in the block, such as the needle core biopsy. > Bottom line on this issue is to educate the pathologist, and not water and stain slides except in rare occasions > > Sent from my iPhone > >> On Aug 17, 2018, at 14:07, Terri Braud wrote: >> >> I'm with Tim Morken on this one. The variability of antigenicity in storage is so wide open, and there really is no recent data, so we just make a point of educating our techs on not wasting tissue/levels during sectioning.? If the techs feel that the residual tissue in the block is in danger of being exhausted, we communicate with our pathologists on how best to handle any requests.? Unstained slides was time, money, and storage and we are better off without them. >> >> Terri L. Braud, HT(ASCP) >> Anatomic Pathology Supervisor >> Laboratory >> Holy Redeemer Hospital >> 1648 Huntingdon Pike >> Meadowbrook, PA 19046 >> ph: 215-938-3689 >> fax: 215-938-3874 >> Care, Comfort, and Heal >> >> Today's Topics: >> 7. Re: Unstained slides - how long are they good for? >>? ? (Morken, Timothy) >> >> Message: 7 >> Date: Fri, 17 Aug 2018 15:16:00 +0000 >> From: "Morken, Timothy" >> To: P Sicurello >> Subject: Re: [Histonet] Unstained slides - how long are they good for? >> >> >> Paula, since it is variable we strive to not have unstained slides. We had kept them indefinitely, then when storage was overwhelming us we reduced it to 2 months maximum. Now we require request for unstained to be ordered in the system and delivered to the pathologist. We do not hold any in the lab. We recut when new stains are ordered. In the past we had routinely cut extras "just in case" but ended up with thousands of unstained slides that were never used. Instead we trained everyone to reduce wastage and get good sections from a cut block with minimal facing. We have not stored unstained sections for many years and they do not seem to be missed. >> >> Tim Morken >> Pathology Site Manager, Parnassus >> Supervisor, Electron Microscopy/Neuromuscular Special Studies >> Department of Pathology UC San Francisco Medical Center >> >> >> -----Original Message----- >> From: P Sicurello via Histonet >> [mailto:histonet at lists.utsouthwestern.edu] >> Sent: Thursday, August 16, 2018 4:49 PM >> To: HistoNet >> Subject: [Histonet] Unstained slides - how long are they good for? >> >> Hello My Fellow Histologists, >> >> Happy Friday Eve. >> >> The question has come up......? How long are *unstained* slides good for? >> Not for H&E but tests like IHC and molecular testing.? These slides >> have been cut, stored at room temperature, not sealed in anyway, and >> kept in a cardboard box. >> >> Please let me know what your opinions are and what your retention >> policy is concerning *unstained* slides. >> >> Thanks oodles. >> >> Sincerely, >> >> Paula Sicurello, HTL (ASCP)CM >> >> Histotechnology Specialist >> >> UC San Diego Health >> >> 200 Arbor Drive >> >> San Diego, CA 92103 >> >> (P): 619-543-2872 >> >> >> >> *Confidentiality Notice*: The information transmitted in this e-mail >> is intended only for the person or entity to which it is addressed and >> may contain confidential and/or privileged material.? Any review, >> retransmission, dissemination or other use of or taking of any action >> in reliance upon this information by persons or entities other than >> the intended recipient is prohibited.? If you received this e-mail in >> error, please contact the sender and delete the material from any computer. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste >> rn.edu_mailman_listinfo_histonet&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVv >> RFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=Di_I >> rEz2lxTIgibc_SPWiFaTGmnw4SYfIOvpPRy-TDM&s=4L8HS7D7SpEHBR1sDxuVWj8zyMgT >> _QNsAkdyu2fu2P4&e= >> >> >> >> ------------------------------ >> >> Subject: Digest Footer >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste >> rn.edu_mailman_listinfo_histonet&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVv >> RFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=Di_I >> rEz2lxTIgibc_SPWiFaTGmnw4SYfIOvpPRy-TDM&s=4L8HS7D7SpEHBR1sDxuVWj8zyMgT >> _QNsAkdyu2fu2P4&e= >> >> ------------------------------ >> >> End of Histonet Digest, Vol 177, Issue 16 >> ***************************************** >> >> >> > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=Di_IrEz2lxTIgibc_SPWiFaTGmnw4SYfIOvpPRy-TDM&s=4L8HS7D7SpEHBR1sDxuVWj8zyMgT_QNsAkdyu2fu2P4&e= > > > > > |? | Virus-free. > | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.avast.com&d=Dw > | IGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPkn > | IMjMlCkk-KGKHflslRsFz3l5JE&m=Di_IrEz2lxTIgibc_SPWiFaTGmnw4SYfIOvpPRy-T > | DM&s=QSBL3lVp0DYppOhOIx51poBgB1vYHNFN4lWV9DhKvGc&e=? | > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=e_HtEeZEQXP5NUOb33qoTj0AVvRFBS9_rhBTQcfkWoA&r=eCMS5E4UqfGKTlPknIMjMlCkk-KGKHflslRsFz3l5JE&m=Di_IrEz2lxTIgibc_SPWiFaTGmnw4SYfIOvpPRy-TDM&s=4L8HS7D7SpEHBR1sDxuVWj8zyMgT_QNsAkdyu2fu2P4&e= > > > Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. | | Virus-free. www.avast.com | From carl.hobbs at kcl.ac.uk Mon Sep 3 13:54:23 2018 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Mon, 3 Sep 2018 18:54:23 +0000 Subject: [Histonet] Unstained slides Message-ID: I agree: cut only the sections needed. Saves space. Sure, you lose several sections of tissue when cutting more sections. That is acceptable because, if this "oxidation" theory is true, then the initial sections will be no good. However, careful organisation of exptl procedure before actual cutting will work very well. Actually, not many Ags get "oxidised"....for eg: I can demonstrate GFAP in sections that are a year old ( sure, they are stored at 4C just in case) These slides are used for Yr 1 BSc practicals and are consistently positive. Nobody knows why some Ags ( and not others) lose their antigenicity, imho Oxidation is a vague reasoning. Just like nobody really knows why HIER works: however, I am in the dipole moment school of thought, rather than the Ca++ skool Sure, in Formalin-fixed specimens. Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From Linda.Margraf at cookchildrens.org Tue Sep 4 11:00:15 2018 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Tue, 4 Sep 2018 16:00:15 +0000 Subject: [Histonet] R and D Manager position Message-ID: <92ee86186caf4d9d9240cb2c0ff402af@MBX10.CCHCS.LDAP> Here is a message I am posting for a non-list member. Please respond to them not me, thanks! Folio Biosciences and Conversant Bio have merged to create the life science industry's premier provider of research services and biospecimen solutions. We're looking for a standout Manager, Research and Development Laboratory, who will oversee all operations in our Histology Lab. Additionally, the Manager will support our immunohistochemistry (IHC) clinical trials. Are you someone with the unique combination of strong supervisory and histology skills who's looking for a future with a growing biotech company? To be successful in this role, you must have a minimum of 3-5 years of experience in immunohistochemistry (IHC) and/or histology, have strong organizational and supervisory skills, keen attention to detail, excellent laboratory skills and be an excellent communicator and team player. ASCP Histology Technician Certification is preferred. Previous work experience in a College of American Pathologists or CLIA environment is also preferred. You must be able to work in a team that values communication, embraces challenge, innovation and continuous improvement, works hard and loves to have fun, is fast-paced and displays a can-do attitude. If this is you and you can prove it, you could be a great fit for our team. We offer excellent pay and benefits, an unbeatable work environment, and the chance to make a difference in the lives of patients. We hire based on talent and fit. Apply only if you meet our performance and ability guidelines. You can find us at www.conversantbio.com/jobs EEO/ADA For more information on Folio Conversant, visit www.folioconversant.com From bakevictoria at gmail.com Tue Sep 4 11:32:37 2018 From: bakevictoria at gmail.com (Victoria Baker) Date: Tue, 4 Sep 2018 12:32:37 -0400 Subject: [Histonet] HIF-1alpha and EBNA1 Message-ID: To All, I am seaching for a lab that can run both of these antibodies on human FFPE tissue. HIF-1 alpha is Hypoxia Inducible 1 alpha EBNA1 is Epstein Barr Nuclear Antigen 1 If anyone performs these tests or knows of a lab that does, please contact me. Thank you in advance Vikki From Ana.Maluenda at baker.edu.au Tue Sep 4 20:34:47 2018 From: Ana.Maluenda at baker.edu.au (Ana Maluenda) Date: Wed, 5 Sep 2018 01:34:47 +0000 Subject: [Histonet] Unstained slides In-Reply-To: References: Message-ID: Hi all, It has been very interesting reading all your comments on unstained slides. This has been a forever discussion I always have everywhere I go in different research institutes. So expanding the topic, I wonder what's everyone's opinion on unstained cryosections? How long are they reliable to be used for IHC? Kind regards, Ana Ana Maluenda Research Assistant Atherothrombosis and Vascular Biology Laboratory Baker Heart and Diabetes Institute 75 Commercial Road, Melbourne VIC 3004 P (03) 8532 1359 E Ana.Maluenda at baker.edu.au W www.baker.edu.au -----Original Message----- From: Hobbs, Carl [mailto:carl.hobbs at kcl.ac.uk] Sent: Tuesday, 4 September 2018 4:54 AM To: histonet Subject: Re: [Histonet] Unstained slides I agree: cut only the sections needed. Saves space. Sure, you lose several sections of tissue when cutting more sections. That is acceptable because, if this "oxidation" theory is true, then the initial sections will be no good. However, careful organisation of exptl procedure before actual cutting will work very well. Actually, not many Ags get "oxidised"....for eg: I can demonstrate GFAP in sections that are a year old ( sure, they are stored at 4C just in case) These slides are used for Yr 1 BSc practicals and are consistently positive. Nobody knows why some Ags ( and not others) lose their antigenicity, imho Oxidation is a vague reasoning. Just like nobody really knows why HIER works: however, I am in the dipole moment school of thought, rather than the Ca++ skool Sure, in Formalin-fixed specimens. Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting privacy at baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg From hmarlatt26 at gmail.com Tue Sep 4 23:06:52 2018 From: hmarlatt26 at gmail.com (Heather Marlatt) Date: Tue, 4 Sep 2018 22:06:52 -0600 Subject: [Histonet] Seeking On call Histotech Message-ID: Hello Histonet! We are seeking an ?on call? histotech to help us through our busy season and potentially longer. Our lab is located in Spokane Valley, Wa and it?s a fun relaxed environment. The schedule is very flexible and pay negotiable. We are a veterinary and research focused lab but welcome all backgrounds. It?s a very unique position with a lot of growth opportunity. Please send resumes to nationwidehistology at gmail.com Thank you, Heather Marlatt From jhill at vet.k-state.edu Wed Sep 5 12:18:46 2018 From: jhill at vet.k-state.edu (Jennifer Phinney) Date: Wed, 5 Sep 2018 17:18:46 +0000 Subject: [Histonet] Elephant Tissues Message-ID: Hello Histonetters, Does anyone have experience processing and cutting elephant tissues? Any tips, tricks, or advice? My lab has had some elephant cases recently and the tissues are unexpectedly (to us) difficult to cut. Thanks for any help, Jennifer Phinney QIHC Kansas State University Veterinary Diagnostic Lab From bcdukes at lexhealth.org Wed Sep 5 12:53:26 2018 From: bcdukes at lexhealth.org (Blake Taylor) Date: Wed, 5 Sep 2018 17:53:26 +0000 Subject: [Histonet] Temperature for Storing Slides and Blocks Message-ID: Is there a good reference or standard on what the room temperature should be for storing slides and blocks? We have moved our long term storage out to our hospitals warehouse and I believe the building is getting much too hot. What temperature range is everyone using? Thanks so much Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes at lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use to the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. From eddessa at emory.edu Wed Sep 5 13:41:24 2018 From: eddessa at emory.edu (Dessasau III, Evan) Date: Wed, 5 Sep 2018 18:41:24 +0000 Subject: [Histonet] Temperature for Storing Slides and Blocks In-Reply-To: References: Message-ID: When we had our structure built we had cooling/heating units(2) put in a structure that is about 818sqft. 70F summer and 65 to 68F winter is what we aim for. Not sure if that is ideal but it keeps the blocks cool in the summer and the slides from sticking in the winter. Thank you, E-van Histology Lab, Yerkes Rm. 2122 7-7744 Lab 7-7902 office -----Original Message----- From: Blake Taylor via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, September 5, 2018 1:53 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Temperature for Storing Slides and Blocks Is there a good reference or standard on what the room temperature should be for storing slides and blocks? We have moved our long term storage out to our hospitals warehouse and I believe the building is getting much too hot. What temperature range is everyone using? Thanks so much Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes at lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use to the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From wdesalvo.cac at outlook.com Wed Sep 5 14:27:09 2018 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Wed, 5 Sep 2018 19:27:09 +0000 Subject: [Histonet] Temperature for Storing Slides and Blocks In-Reply-To: References: , Message-ID: I do not know of a standard, but you should make sure the storage temp is 5-10 degrees lower(maybe more depending on fluctuations in temp for the area). That would be < 120F degrees. Request the location Facilities department understands you need temperature control William DeSalvo ________________________________ From: Dessasau III, Evan via Histonet Sent: Wednesday, September 5, 2018 11:52 AM To: Blake Taylor Cc: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Temperature for Storing Slides and Blocks When we had our structure built we had cooling/heating units(2) put in a structure that is about 818sqft. 70F summer and 65 to 68F winter is what we aim for. Not sure if that is ideal but it keeps the blocks cool in the summer and the slides from sticking in the winter. Thank you, E-van Histology Lab, Yerkes Rm. 2122 7-7744 Lab 7-7902 office -----Original Message----- From: Blake Taylor via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, September 5, 2018 1:53 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Temperature for Storing Slides and Blocks Is there a good reference or standard on what the room temperature should be for storing slides and blocks? We have moved our long term storage out to our hospitals warehouse and I believe the building is getting much too hot. What temperature range is everyone using? Thanks so much Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes at lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use to the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7C%7Cc2d150646fa24c6be41908d61360bbab%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636717703515827650&sdata=%2Bu563Zv101RbhGL7eHFfIk2NZSw%2FyHlop62iRlR6BXY%3D&reserved=0 ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7C%7Cc2d150646fa24c6be41908d61360bbab%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636717703515827650&sdata=%2Bu563Zv101RbhGL7eHFfIk2NZSw%2FyHlop62iRlR6BXY%3D&reserved=0 From carl.hobbs at kcl.ac.uk Wed Sep 5 14:55:56 2018 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Wed, 5 Sep 2018 19:55:56 +0000 Subject: [Histonet] Unstained slides Message-ID: Hi Depends on what you mean by cryosections. Unfixed/fixed? Stored at RT, 4C, -20C, -80C. Stored dry or in glycerol So many variables! My opinion is to store blocks and cut sections as required. Least variables. Sure, one loses some tissue everytime one cuts anew....a good thing. It IS complicated so, a project has to be thought out well in advance. Stimulating post Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From akemiat3377 at gmail.com Wed Sep 5 15:59:03 2018 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Wed, 5 Sep 2018 13:59:03 -0700 Subject: [Histonet] Alcian Blue stain on lab coats Message-ID: <86700546-0D40-4B5A-84FE-401FB3E0D16F@gmail.com> Hello Histo Peeps: I have two new lab coats which have been stained with Alcian Blue and can?t get the stains out. I have tried oxy clean incorporated with bleach, as well as pre-soaking in 3% acetic acid, and American Mastertech?s stain remover to no avail. Any suggestions would be greatly appreciated. Thank you in advance, Akemi Allison, BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com Tele: (831) 375-3577 X117 From patpxs at gmail.com Wed Sep 5 16:42:39 2018 From: patpxs at gmail.com (P Sicurello) Date: Wed, 5 Sep 2018 14:42:39 -0700 Subject: [Histonet] Unstained slides In-Reply-To: References: Message-ID: How about frozen sections cut for immunofluorescence stored at -20? Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Wed, Sep 5, 2018 at 1:12 PM Hobbs, Carl via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > Hi > > Depends on what you mean by cryosections. > Unfixed/fixed? > Stored at RT, 4C, -20C, -80C. > Stored dry or in glycerol > > So many variables! > My opinion is to store blocks and cut sections as required. > Least variables. > Sure, one loses some tissue everytime one cuts anew....a good thing. > > It IS complicated so, a project has to be thought out well in advance. > > Stimulating post > > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > 020 7848 6813 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From patpxs at gmail.com Wed Sep 5 16:46:06 2018 From: patpxs at gmail.com (P Sicurello) Date: Wed, 5 Sep 2018 14:46:06 -0700 Subject: [Histonet] Elephant Tissues In-Reply-To: References: Message-ID: Jennifer, I have worked on mouse, rat, rabbit, sea lion, harbor seal, killer whale, giraffe, and even human mummy tissues. With the exception of the mummy tissue being a bit dry, they all embedded and cut like human tissue. What is it that is making them hard to cut? Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Wed, Sep 5, 2018 at 10:38 AM Jennifer Phinney via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello Histonetters, > Does anyone have experience processing and cutting elephant tissues? Any > tips, tricks, or advice? My lab has had some elephant cases recently and > the tissues are unexpectedly (to us) difficult to cut. > > Thanks for any help, > Jennifer Phinney QIHC > Kansas State University > Veterinary Diagnostic Lab > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From patpxs at gmail.com Wed Sep 5 17:05:04 2018 From: patpxs at gmail.com (P Sicurello) Date: Wed, 5 Sep 2018 15:05:04 -0700 Subject: [Histonet] I have another question for the collective Message-ID: Good Afternoon Listers, Here's a question for those who work in the academic clinical arena. Background: We have researchers who have IRBs in place. They sign a patient up for their study. The researcher wants slides cut on patient tissue to use for their study. They want it right away (of course). Question: Do you charge them extra when they want their slides rushed? When I worked in research (almost 10 years ago) I charged extra for rush cases: $50 for next day, $100 for same day. Thanks in advance! Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive San Diego, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From JosephE at mcclainlab.com Thu Sep 6 09:22:35 2018 From: JosephE at mcclainlab.com (Joseph Esposito) Date: Thu, 6 Sep 2018 14:22:35 +0000 Subject: [Histonet] Giemsa vs Diff Quik Message-ID: The laboratory I work at has been using the Diff Quik for years now as a stain for fine needle aspirates. Recently, when we tried to reorder a Diff Quik stain kit from our usual suppliers, we have found it to be on backorder. This has caused us to begin to consider the Giemsa stain as an alternative to the Diff Quik. Does anyone have any experience with using both stains and how they compare to each other? Would the Giemsa be a suitable alternative to replace the Diff Quik? Joseph A. Esposito McClain Laboratories, LLC. (631) 361- 4000 From jhill at vet.k-state.edu Thu Sep 6 09:41:48 2018 From: jhill at vet.k-state.edu (Jennifer Phinney) Date: Thu, 6 Sep 2018 14:41:48 +0000 Subject: [Histonet] Elephant Tissues In-Reply-To: References: Message-ID: Hi Paula, One of my pathologists thinks it could be the collagen in the tissues making them difficult to cut. We have tried nair, fabric softener, and even decaling the tissues and nothing helps. The tissues shred immediately when trying to section making it impossible in some cases to actually get a slide. When I melted a particularly difficult block down to separate out the different tissues, it turned out to be the lymph node that was the worst. We are going to try some of the cream you use to soften scar tissues to see if that has any effect. As a veterinary histology lab we routinely work on a variety of different species, and so far the elephant tissue is the only one giving us problems. Thanks for everyone?s help, Jennifer From: P Sicurello Sent: Wednesday, September 5, 2018 4:46 PM To: Jennifer Phinney Cc: HistoNet Subject: Re: [Histonet] Elephant Tissues Jennifer, I have worked on mouse, rat, rabbit, sea lion, harbor seal, killer whale, giraffe, and even human mummy tissues. With the exception of the mummy tissue being a bit dry, they all embedded and cut like human tissue. What is it that is making them hard to cut? Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 Confidentiality Notice: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Wed, Sep 5, 2018 at 10:38 AM Jennifer Phinney via Histonet > wrote: Hello Histonetters, Does anyone have experience processing and cutting elephant tissues? Any tips, tricks, or advice? My lab has had some elephant cases recently and the tissues are unexpectedly (to us) difficult to cut. Thanks for any help, Jennifer Phinney QIHC Kansas State University Veterinary Diagnostic Lab _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From boznpl at aol.com Thu Sep 6 10:01:00 2018 From: boznpl at aol.com (Laurie Colbert) Date: Thu, 6 Sep 2018 11:01:00 -0400 Subject: [Histonet] Giemsa vs Diff Quik In-Reply-To: References: Message-ID: <165af669483-1ebc-9f4@webjasstg-vab47.srv.aolmail.net> We use the Diff Quik in place of the Giemsa stain for H. Pylori on gastric bx's.? I believe you can use the Diff Quik for FNA's, especially if it is just for a preliminary diagnosis and you will be staining with H&E or a cytology stain later.? We get our Diff Quik from Medical Chemical Corporation. Laurie Redmond -----Original Message----- From: Joseph Esposito via Histonet To: histonet Sent: Thu, Sep 6, 2018 7:42 am Subject: [Histonet] Giemsa vs Diff Quik The laboratory I work at has been using the Diff Quik for years now as a stain for fine needle aspirates. Recently, when we tried to reorder a Diff Quik stain kit from our usual suppliers, we have found it to be on backorder. This has caused us to begin to consider the Giemsa stain as an alternative to the Diff Quik. Does anyone have any experience with using both stains and how they compare to each other? Would the Giemsa be a suitable alternative to replace the Diff Quik? Joseph A. Esposito McClain Laboratories, LLC. (631) 361- 4000 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 at gmail.com Thu Sep 6 10:14:36 2018 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Thu, 6 Sep 2018 08:14:36 -0700 Subject: [Histonet] Stain removal on clothing Message-ID: Just found this info on-line for stain removal. I assume it originally came from Chuck Churukian since it?s from University of Rochester. REMOVING STAINS FROM HANDS AND CLOTHING Some stains can be removed form the hands by using *Erado-Sol. Dilute Chlorox (or similar bleach) and Erado-Sol are useful for removing stains from white clothing. Care should be exercised in removing stains from colored clothing. To remove the following stains use the solutions indicated. 1. Alcian blue - to remove from the hands use the acid alcohol solution. For cleaning glassware try using dilute Chlorox or acid alcohol. If the stain resists, use them alternatively. 2. Auramine-rhodamine - acid alcohol. 3. Basic fuchsin - Erado-Sol. 4. Carmine - acid alcohol. 5. Chromic acid - concentrated aqueous sodium thiosulfate containing a few drops of sulfuric acid. 6. Crystal violet - Erado-Sol or Chlorox. 7. Eosin Y - saturated aqueous lithium carbonate. 8. Fast green and similar acid dyes - saturated aqueous lithium carbonate. 9. Hematoxylin (alum and iron) - Erado-Sol or acid alcohol. 10. Iodine - 5% sodium thiosulfate. 11. Methylene blue - acid alcohol, Erado-Sol or Chlorox. 12. Nuclear fast red - acid alcohol. 13. Picric acid - saturated aqueous lithium carbonate. 14. Potassium permanganate - 5% oxalic acid. 15. Schiffs solution - Erado-Sol or Chlorox. 16. Silver deposits - Lugols iodine followed by 5% sodium thiosulfate. 17. Sudan stains (Oil red O, Sudan black B) - acetone. 18. Thioflavin T - acid alcohol. *American Scientific Products From mtoole at dcol.net Thu Sep 6 10:18:23 2018 From: mtoole at dcol.net (Mike Toole) Date: Thu, 6 Sep 2018 10:18:23 -0500 Subject: [Histonet] Giemsa vs Diff Quik In-Reply-To: <165af669483-1ebc-9f4@webjasstg-vab47.srv.aolmail.net> References: <165af669483-1ebc-9f4@webjasstg-vab47.srv.aolmail.net> Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB358D829F127@mail> I ordered this a few weeks ago: http://www.labsco.com/items/EKI2295 STAIN BRI KT 16OZ KT 16OZ / 500ML DIFFERENTIAL RAPID BLOOD STAIN SOLUTION KIT, FOR DIFFERENTIAL STAINING OF BLOOD SMEARS, INCLUDES: XANTHENE DYE SOLUTION (A), THIAZINE DYE SOLUTION (B), FIXATIVE SOLUTION (C), 3 X 16OZ HAZMAT Item Id: EKI2295 Supplier Part Number 2295-500ML Supplier Name E K INDUSTRIES, INC. Stock 14 Availability Item in stock. Ships within one business day. Mike -----Original Message----- From: Laurie Colbert via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 06, 2018 10:01 AM To: JosephE at mcclainlab.com; histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Giemsa vs Diff Quik We use the Diff Quik in place of the Giemsa stain for H. Pylori on gastric bx's.? I believe you can use the Diff Quik for FNA's, especially if it is just for a preliminary diagnosis and you will be staining with H&E or a cytology stain later.? We get our Diff Quik from Medical Chemical Corporation. Laurie Redmond -----Original Message----- From: Joseph Esposito via Histonet To: histonet Sent: Thu, Sep 6, 2018 7:42 am Subject: [Histonet] Giemsa vs Diff Quik The laboratory I work at has been using the Diff Quik for years now as a stain for fine needle aspirates. Recently, when we tried to reorder a Diff Quik stain kit from our usual suppliers, we have found it to be on backorder. This has caused us to begin to consider the Giemsa stain as an alternative to the Diff Quik. Does anyone have any experience with using both stains and how they compare to each other? Would the Giemsa be a suitable alternative to replace the Diff Quik? Joseph A. Esposito McClain Laboratories, LLC. (631) 361- 4000 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtoole at dcol.net Thu Sep 6 11:06:59 2018 From: mtoole at dcol.net (Mike Toole) Date: Thu, 6 Sep 2018 11:06:59 -0500 Subject: [Histonet] Giemsa vs Diff Quik In-Reply-To: <31530E35E0BAB044B3B56B7FE5CF4EB358D829F127@mail> References: <165af669483-1ebc-9f4@webjasstg-vab47.srv.aolmail.net> <31530E35E0BAB044B3B56B7FE5CF4EB358D829F127@mail> Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB358D829F156@mail> For ordering the Diff-Quik alternative this link works better. It takes you directly to the manufacturer EKI. https://eki-chem.com/product/2295 The labsco link below goes to McKesson. Mike -----Original Message----- From: Mike Toole via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 06, 2018 10:18 AM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Giemsa vs Diff Quik I ordered this a few weeks ago: http://www.labsco.com/items/EKI2295 STAIN BRI KT 16OZ KT 16OZ / 500ML DIFFERENTIAL RAPID BLOOD STAIN SOLUTION KIT, FOR DIFFERENTIAL STAINING OF BLOOD SMEARS, INCLUDES: XANTHENE DYE SOLUTION (A), THIAZINE DYE SOLUTION (B), FIXATIVE SOLUTION (C), 3 X 16OZ HAZMAT Item Id: EKI2295 Supplier Part Number 2295-500ML Supplier Name E K INDUSTRIES, INC. Stock 14 Availability Item in stock. Ships within one business day. Mike -----Original Message----- From: Laurie Colbert via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 06, 2018 10:01 AM To: JosephE at mcclainlab.com; histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Giemsa vs Diff Quik We use the Diff Quik in place of the Giemsa stain for H. Pylori on gastric bx's.? I believe you can use the Diff Quik for FNA's, especially if it is just for a preliminary diagnosis and you will be staining with H&E or a cytology stain later.? We get our Diff Quik from Medical Chemical Corporation. Laurie Redmond -----Original Message----- From: Joseph Esposito via Histonet To: histonet Sent: Thu, Sep 6, 2018 7:42 am Subject: [Histonet] Giemsa vs Diff Quik The laboratory I work at has been using the Diff Quik for years now as a stain for fine needle aspirates. Recently, when we tried to reorder a Diff Quik stain kit from our usual suppliers, we have found it to be on backorder. This has caused us to begin to consider the Giemsa stain as an alternative to the Diff Quik. Does anyone have any experience with using both stains and how they compare to each other? Would the Giemsa be a suitable alternative to replace the Diff Quik? Joseph A. Esposito McClain Laboratories, LLC. (631) 361- 4000 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Thu Sep 6 11:41:10 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 6 Sep 2018 16:41:10 +0000 Subject: [Histonet] Elephant skin Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF6EA18@HRHEX02-HOS.holyredeemer.local> Probably one of those things best embedded/cut in plastic. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From ctorrence at kmcpa.com Thu Sep 6 11:43:47 2018 From: ctorrence at kmcpa.com (Carol Torrence) Date: Thu, 6 Sep 2018 16:43:47 +0000 Subject: [Histonet] Pregnancy guide for working in histology Message-ID: Good morning! Could some of you chime in on guidelines you go by for those employee that are expecting a baby. I have removed employee from xylene exposure during staining and cover slipping but am on the fence about grossing. At this time the employee has been removed from grossing. All grossing is done under an exhaust hood. Our exposure badges have always read well below limits. Thanks in advance! Carol M. Torrence, HT(ASCP) From rjbuesa at yahoo.com Thu Sep 6 12:21:00 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 6 Sep 2018 17:21:00 +0000 (UTC) Subject: [Histonet] Pregnancy guide for working in histology In-Reply-To: References: Message-ID: <2112362188.586296.1536254460516@mail.yahoo.com> Both formalin and xylene (and any other dangerous fumes) have to be avoided during pregnancy BUT if you have an efficient fumes hood to do grossing, then you should monitor exposure. Somebody NOT pregnant should gross with a personal formalin badge and, depending on the exposure result, then you may allow the pregnant employee to do grossing or not.Ren? On Thursday, September 6, 2018 1:08 PM, Carol Torrence via Histonet wrote: Good morning! Could some of you chime in on guidelines you go by for those employee that are expecting a baby.? I have removed employee from xylene exposure during staining and cover slipping but am on the fence about grossing.? At this time the employee has been removed from grossing.? All grossing is done under an exhaust hood.? Our exposure badges have always read well below limits.? Thanks in advance! Carol M. Torrence, HT(ASCP) _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Thu Sep 6 12:52:36 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 6 Sep 2018 17:52:36 +0000 Subject: [Histonet] Pregnancy guide Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF6EA44@HRHEX02-HOS.holyredeemer.local> Remember that contamination can also be by skin absorption. With that in mind, we provide thick Nitrile gloves with instruction to change them at least every 30 minutes, or less if they show any signs of deterioration. We have great ventilation and beyond that, when we wear our fume badges, we perform the worst exposure tasks, so when calculated, the exposure shows what it would be like if we did that task for 8 hours a day - and we STILL are way under the limit. The result: No restrictions of duties - 5 babies born to employees here without a single problem in 12 years. However, with that said, I would never ask a pregnant technician to hand coverslip more than the occasional slide. Just my 2cents - T Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 2. Pregnancy guide for working in histology (Carol Torrence) Message: 2 Date: Thu, 6 Sep 2018 16:43:47 +0000 From: Carol Torrence To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Pregnancy guide for working in histology Message-ID: Content-Type: text/plain; charset="us-ascii" Good morning! Could some of you chime in on guidelines you go by for those employee that are expecting a baby. I have removed employee from xylene exposure during staining and cover slipping but am on the fence about grossing. At this time the employee has been removed from grossing. All grossing is done under an exhaust hood. Our exposure badges have always read well below limits. Thanks in advance! Carol M. Torrence, HT(ASCP) From rsrichmond at gmail.com Thu Sep 6 15:37:43 2018 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 6 Sep 2018 16:37:43 -0400 Subject: [Histonet] Giemsa vs Diff Quik In-Reply-To: References: Message-ID: Joseph A. Esposito at McClain Laboratories on Long Island asks: >>The laboratory I work at has been using the Diff Quik for years now as a stain for fine needle aspirates. Recently, when we tried to reorder a Diff Quik stain kit from our usual suppliers, we have found it to be on backorder. This has caused us to begin to consider the Giemsa stain as an alternative to the Diff Quik. Does anyone have any experience with using both stains and how they compare to each other? Would the Giemsa be a suitable alternative to replace the Diff Quik?<< Diff-Quik? (spelt like that!) is an old registered trademark for a rapid two-step Romanowsky stain. A number of Web sites inform me today that this trade name has been discontinued, I suppose because the name has been so commonly used generically. There have long been a number of generic fast two-step Romanowsky stains available, with a xanthene dye (presumably eosin Y) as solution 1, and a proprietary mixture of thiazine dyes as solution 2. In my experience with at least five of these, they all worked pretty much indistinguishably from brand-name Diff-Quik. There are a number of stains called Giemsa, also Romanowsky stains, but often slower than the "quick" stains. There is probably no advantage to using them for any reason. In my eightieth year, I remember with nostalgia the old Wolbach Giemsa technique for tissue sections. Tissue must be fixed in a dichromate fixative, traditionally Zenker/Helly, sections stained in four successive Giemsa baths, the last one overnight, and differentiated with 10% colophonium rosin in alcohol with microscopic control. (I've actually done this stain myself.) Bob Richmond Samurai Pathologist Maryville TN From boznpl at aol.com Thu Sep 6 21:23:34 2018 From: boznpl at aol.com (Laurie Colbert) Date: Thu, 6 Sep 2018 22:23:34 -0400 Subject: [Histonet] Paraffin Dispenser Message-ID: <165b1d77c6c-1eb8-2d60@webjasstg-vab23.srv.aolmail.net> I'm looking for a 2.5 gallon (approx) paraffin dispenser ASAP - new or refurbished.? I need to find one that is in stock and can be shipped immediately.? Vendors are welcome to respond. Thank you, Laurie Redmond Path MD? Los Angeles, CA From katelin09htl at gmail.com Thu Sep 6 22:15:49 2018 From: katelin09htl at gmail.com (Katelin Tellechea) Date: Thu, 6 Sep 2018 20:15:49 -0700 Subject: [Histonet] Pregnancy guide for working in histology In-Reply-To: References: Message-ID: Hi Carol, I did a poster presentation on this topic at the NSH Symposium in Long Beach, 2016. I would be happy to send you a copy of my poster. I also wrote a blog article about my research for the poster found here: https://www.fixationonhistology.com/home/my-pregnancy-in-the-lab-researching-safety-considerations The Block also has the abstract, podcast interview, and a Safety Snippet of my poster. Please contact me directly if you have any other questions or I can help in any way. Katelin On Thu, Sep 6, 2018, 10:01 AM Carol Torrence via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good morning! > > Could some of you chime in on guidelines you go by for those employee that > are expecting a baby. I have removed employee from xylene exposure during > staining and cover slipping but am on the fence about grossing. At this > time the employee has been removed from grossing. All grossing is done > under an exhaust hood. Our exposure badges have always read well below > limits. Thanks in advance! > > Carol M. Torrence, HT(ASCP) > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Histology at nwlabs.co.uk Fri Sep 7 01:06:32 2018 From: Histology at nwlabs.co.uk (Histology) Date: Fri, 7 Sep 2018 06:06:32 +0000 Subject: [Histonet] Elephant Tissues Message-ID: Hi Jennifer, If it is skin extending the fixing and processing time should cure any processing issues. If after this it?s still not working a short pre fix in khales fixative and surface softening before sectioning with 0.02% sodium hydroxide may help. Hope this helps! Stuart Beaver BSc(Hons) Head of Veterinary Histology/Cytology +447568543761 On 5 Sep 2018, at 22:46, P Sicurello > wrote: Jennifer, I have worked on mouse, rat, rabbit, sea lion, harbor seal, killer whale, giraffe, and even human mummy tissues. With the exception of the mummy tissue being a bit dry, they all embedded and cut like human tissue. What is it that is making them hard to cut? Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Wed, Sep 5, 2018 at 10:38 AM Jennifer Phinney via Histonet < histonet at lists.utsouthwestern.edu> wrote: Hello Histonetters, Does anyone have experience processing and cutting elephant tissues? Any tips, tricks, or advice? My lab has had some elephant cases recently and the tissues are unexpectedly (to us) difficult to cut. Thanks for any help, Jennifer Phinney QIHC Kansas State University Veterinary Diagnostic Lab _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michael.gudo at morphisto.de Fri Sep 7 02:56:00 2018 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Fri, 7 Sep 2018 09:56:00 +0200 Subject: [Histonet] Elephant Tissues In-Reply-To: References: Message-ID: <3F945ADB-2465-44A3-8342-EDCF98917267@morphisto.de> Hello Jennifer, I do not have experiences with elephant tissues, but with other tissues that have thick connective fibres. Sodium hydroxide may help, however, I would recommend resin embedding, too. For this purpose Technovit 7100 would be a good and easy to use resin. Its easy to infiltrate and can be cut with rotary microtomes with some special knifes, and there are many stains that work on Technovit 7100 embedded specimens. Please let me know, if you need further information. We have a local dealer in US. Kind regards Michael > Am 06.09.2018 um 16:41 schrieb Jennifer Phinney via Histonet : > > Hi Paula, > One of my pathologists thinks it could be the collagen in the tissues making them difficult to cut. We have tried nair, fabric softener, and even decaling the tissues and nothing helps. The tissues shred immediately when trying to section making it impossible in some cases to actually get a slide. When I melted a particularly difficult block down to separate out the different tissues, it turned out to be the lymph node that was the worst. > > We are going to try some of the cream you use to soften scar tissues to see if that has any effect. > > As a veterinary histology lab we routinely work on a variety of different species, and so far the elephant tissue is the only one giving us problems. > > Thanks for everyone?s help, > Jennifer > > From: P Sicurello > Sent: Wednesday, September 5, 2018 4:46 PM > To: Jennifer Phinney > Cc: HistoNet > Subject: Re: [Histonet] Elephant Tissues > > Jennifer, > > I have worked on mouse, rat, rabbit, sea lion, harbor seal, killer whale, giraffe, and even human mummy tissues. With the exception of the mummy tissue being a bit dry, they all embedded and cut like human tissue. > > What is it that is making them hard to cut? > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > Confidentiality Notice: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. > > > On Wed, Sep 5, 2018 at 10:38 AM Jennifer Phinney via Histonet > wrote: > Hello Histonetters, > Does anyone have experience processing and cutting elephant tissues? Any tips, tricks, or advice? My lab has had some elephant cases recently and the tissues are unexpectedly (to us) difficult to cut. > > Thanks for any help, > Jennifer Phinney QIHC > Kansas State University > Veterinary Diagnostic Lab > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From annigyg at gmail.com Fri Sep 7 13:12:49 2018 From: annigyg at gmail.com (Anne van Binsbergen) Date: Fri, 7 Sep 2018 19:12:49 +0100 Subject: [Histonet] Kwik Diff Message-ID: <3D9AD531-06CE-451C-AFE1-743A3B94C345@gmail.com> Hi Histonetters Try the Kwik Diff from Thermo Shandon. We have been using it for as long as I can remember. I?m sure there must be suppliers in the USA. Annie in Arabia Sent from my iPhone > On 07 Sep 2018, at 18:00, histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Pregnancy guide for working in histology (Rene J Buesa) > 2. Re: Pregnancy guide (Terri Braud) > 3. Re: Giemsa vs Diff Quik (Bob Richmond) > 4. Paraffin Dispenser (Laurie Colbert) > 5. Re: Pregnancy guide for working in histology (Katelin Tellechea) > 6. Re: Elephant Tissues (Histology) > 7. Re: Elephant Tissues (Dr. Michael Gudo (Morphisto GmbH)) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 6 Sep 2018 17:21:00 +0000 (UTC) > From: Rene J Buesa > To: Carol Torrence , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Pregnancy guide for working in histology > Message-ID: <2112362188.586296.1536254460516 at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > Both formalin and xylene (and any other dangerous fumes) have to be avoided during pregnancy BUT if you have an efficient fumes hood to do grossing, then you should monitor exposure. Somebody NOT pregnant should gross with a personal formalin badge and, depending on the exposure result, then you may allow the pregnant employee to do grossing or not.Ren? > > On Thursday, September 6, 2018 1:08 PM, Carol Torrence via Histonet wrote: > > > Good morning! > > Could some of you chime in on guidelines you go by for those employee that are expecting a baby.? I have removed employee from xylene exposure during staining and cover slipping but am on the fence about grossing.? At this time the employee has been removed from grossing.? All grossing is done under an exhaust hood.? Our exposure badges have always read well below limits.? Thanks in advance! > > Carol M. Torrence, HT(ASCP) > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 2 > Date: Thu, 6 Sep 2018 17:52:36 +0000 > From: "Terri Braud" > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: Re: [Histonet] Pregnancy guide > Message-ID: > <48E053DDF6CE074DB6A7414BA05403F8ECF6EA44 at HRHEX02-HOS.holyredeemer.local> > > Content-Type: text/plain; charset="us-ascii" > > Remember that contamination can also be by skin absorption. With that in mind, we provide thick Nitrile gloves with instruction to change them at least every 30 minutes, or less if they show any signs of deterioration. We have great ventilation and beyond that, when we wear our fume badges, we perform the worst exposure tasks, so when calculated, the exposure shows what it would be like if we did that task for 8 hours a day - and we STILL are way under the limit. The result: No restrictions of duties - 5 babies born to employees here without a single problem in 12 years. > However, with that said, I would never ask a pregnant technician to hand coverslip more than the occasional slide. > Just my 2cents - T > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > > 2. Pregnancy guide for working in histology (Carol Torrence) > Message: 2 > Date: Thu, 6 Sep 2018 16:43:47 +0000 > From: Carol Torrence > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Pregnancy guide for working in histology > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Good morning! > > Could some of you chime in on guidelines you go by for those employee that are expecting a baby. I have removed employee from xylene exposure during staining and cover slipping but am on the fence about grossing. At this time the employee has been removed from grossing. All grossing is done under an exhaust hood. Our exposure badges have always read well below limits. Thanks in advance! > > Carol M. Torrence, HT(ASCP) > > > > > > ------------------------------ > > Message: 3 > Date: Thu, 6 Sep 2018 16:37:43 -0400 > From: Bob Richmond > To: "Histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Giemsa vs Diff Quik > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > Joseph A. Esposito at McClain Laboratories on Long Island asks: > >>> The laboratory I work at has been using the Diff Quik for years now as a > stain for fine needle aspirates. Recently, when we tried to reorder a Diff > Quik stain kit from our usual suppliers, we have found it to be on > backorder. This has caused us to begin to consider the Giemsa stain as an > alternative to the Diff Quik. Does anyone have any experience with using > both stains and how they compare to each other? Would the Giemsa be a > suitable alternative to replace the Diff Quik?<< > > Diff-Quik? (spelt like that!) is an old registered trademark for a rapid > two-step Romanowsky stain. A number of Web sites inform me today that this > trade name has been discontinued, I suppose because the name has been so > commonly used generically. > > There have long been a number of generic fast two-step Romanowsky stains > available, with a xanthene dye (presumably eosin Y) as solution 1, and a > proprietary mixture of thiazine dyes as solution 2. In my experience with > at least five of these, they all worked pretty much indistinguishably from > brand-name Diff-Quik. > > There are a number of stains called Giemsa, also Romanowsky stains, but > often slower than the "quick" stains. There is probably no advantage to > using them for any reason. > > In my eightieth year, I remember with nostalgia the old Wolbach Giemsa > technique for tissue sections. Tissue must be fixed in a dichromate > fixative, traditionally Zenker/Helly, sections stained in four successive > Giemsa baths, the last one overnight, and differentiated with 10% > colophonium rosin in alcohol with microscopic control. (I've actually done > this stain myself.) > > Bob Richmond > Samurai Pathologist > Maryville TN > > > ------------------------------ > > Message: 4 > Date: Thu, 6 Sep 2018 22:23:34 -0400 > From: Laurie Colbert > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Paraffin Dispenser > Message-ID: <165b1d77c6c-1eb8-2d60 at webjasstg-vab23.srv.aolmail.net> > Content-Type: text/plain; charset=utf-8 > > I'm looking for a 2.5 gallon (approx) paraffin dispenser ASAP - new or refurbished.? I need to find one that is in stock and can be shipped immediately.? Vendors are welcome to respond. > > Thank you, > Laurie Redmond > Path MD? > Los Angeles, CA > > ------------------------------ > > Message: 5 > Date: Thu, 6 Sep 2018 20:15:49 -0700 > From: Katelin Tellechea > To: Carol Torrence > Cc: Histonet > Subject: Re: [Histonet] Pregnancy guide for working in histology > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > Hi Carol, > I did a poster presentation on this topic at the NSH Symposium in Long > Beach, 2016. > I would be happy to send you a copy of my poster. I also wrote a blog > article about my research for the poster found here: > https://www.fixationonhistology.com/home/my-pregnancy-in-the-lab-researching-safety-considerations > The Block also has the abstract, podcast interview, and a Safety Snippet of > my poster. > Please contact me directly if you have any other questions or I can help in > any way. > Katelin > > > On Thu, Sep 6, 2018, 10:01 AM Carol Torrence via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Good morning! >> >> Could some of you chime in on guidelines you go by for those employee that >> are expecting a baby. I have removed employee from xylene exposure during >> staining and cover slipping but am on the fence about grossing. At this >> time the employee has been removed from grossing. All grossing is done >> under an exhaust hood. Our exposure badges have always read well below >> limits. Thanks in advance! >> >> Carol M. Torrence, HT(ASCP) >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > ------------------------------ > > Message: 6 > Date: Fri, 7 Sep 2018 06:06:32 +0000 > From: Histology > To: P Sicurello > Cc: "jhill at vet.k-state.edu" , HistoNet > > Subject: Re: [Histonet] Elephant Tissues > Message-ID: > Content-Type: text/plain; charset="utf-8" > > Hi Jennifer, > > If it is skin extending the fixing and processing time should cure any processing issues. If after this it?s still not working a short pre fix in khales fixative and surface softening before sectioning with 0.02% sodium hydroxide may help. > > Hope this helps! > > Stuart Beaver BSc(Hons) > Head of Veterinary Histology/Cytology > +447568543761 > > On 5 Sep 2018, at 22:46, P Sicurello > wrote: > > Jennifer, > > I have worked on mouse, rat, rabbit, sea lion, harbor seal, killer whale, > giraffe, and even human mummy tissues. With the exception of the mummy > tissue being a bit dry, they all embedded and cut like human tissue. > > What is it that is making them hard to cut? > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > > > On Wed, Sep 5, 2018 at 10:38 AM Jennifer Phinney via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > Hello Histonetters, > Does anyone have experience processing and cutting elephant tissues? Any > tips, tricks, or advice? My lab has had some elephant cases recently and > the tissues are unexpectedly (to us) difficult to cut. > > Thanks for any help, > Jennifer Phinney QIHC > Kansas State University > Veterinary Diagnostic Lab > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 7 > Date: Fri, 7 Sep 2018 09:56:00 +0200 > From: "Dr. Michael Gudo (Morphisto GmbH)" > To: Jennifer Phinney > Cc: P Sicurello , HistoNet > > Subject: Re: [Histonet] Elephant Tissues > Message-ID: <3F945ADB-2465-44A3-8342-EDCF98917267 at morphisto.de> > Content-Type: text/plain; charset=utf-8 > > Hello Jennifer, > > I do not have experiences with elephant tissues, but with other tissues that have thick connective fibres. Sodium hydroxide may help, however, I would recommend resin embedding, too. For this purpose Technovit 7100 would be a good and easy to use resin. Its easy to infiltrate and can be cut with rotary microtomes with some special knifes, and there are many stains that work on Technovit 7100 embedded specimens. Please let me know, if you need further information. We have a local dealer in US. > > Kind regards > Michael > >> Am 06.09.2018 um 16:41 schrieb Jennifer Phinney via Histonet : >> >> Hi Paula, >> One of my pathologists thinks it could be the collagen in the tissues making them difficult to cut. We have tried nair, fabric softener, and even decaling the tissues and nothing helps. The tissues shred immediately when trying to section making it impossible in some cases to actually get a slide. When I melted a particularly difficult block down to separate out the different tissues, it turned out to be the lymph node that was the worst. >> >> We are going to try some of the cream you use to soften scar tissues to see if that has any effect. >> >> As a veterinary histology lab we routinely work on a variety of different species, and so far the elephant tissue is the only one giving us problems. >> >> Thanks for everyone?s help, >> Jennifer >> >> From: P Sicurello >> Sent: Wednesday, September 5, 2018 4:46 PM >> To: Jennifer Phinney >> Cc: HistoNet >> Subject: Re: [Histonet] Elephant Tissues >> >> Jennifer, >> >> I have worked on mouse, rat, rabbit, sea lion, harbor seal, killer whale, giraffe, and even human mummy tissues. With the exception of the mummy tissue being a bit dry, they all embedded and cut like human tissue. >> >> What is it that is making them hard to cut? >> >> Sincerely, >> >> Paula Sicurello, HTL (ASCP)CM >> >> Histotechnology Specialist >> >> UC San Diego Health >> >> 200 Arbor Drive >> >> San Diego, CA 92103 >> >> (P): 619-543-2872 >> >> >> >> Confidentiality Notice: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. >> >> >> On Wed, Sep 5, 2018 at 10:38 AM Jennifer Phinney via Histonet > wrote: >> Hello Histonetters, >> Does anyone have experience processing and cutting elephant tissues? Any tips, tricks, or advice? My lab has had some elephant cases recently and the tissues are unexpectedly (to us) difficult to cut. >> >> Thanks for any help, >> Jennifer Phinney QIHC >> Kansas State University >> Veterinary Diagnostic Lab >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ************************************************************************************************ > MORPHISTO GmbH > PD Dr. phil. nat. Michael Gudo > Weism?llerstr. 45 > 60314 Frankfurt am Main > Telefon: 069 / 400 3019 - 62 > Telefax: 069 / 400 3019 - 64 > > E-Mail: michael.gudo at morphisto.de > Internet: http://www.morphisto.de/ > > Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo > > Registergericht: Amtsgericht Frankfurt > Registernummer: HRB 74954 > Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 > ************************************************************************************************ > Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. > ************************************************************************************************ > This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 178, Issue 6 > **************************************** From carl.hobbs at kcl.ac.uk Fri Sep 7 14:04:22 2018 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 7 Sep 2018 19:04:22 +0000 Subject: [Histonet] Elephant Tissues Message-ID: If your tissues are embedded in Pwax and do not cut....sorry! You have XP with many tissues so, as corroborated, elephant should be also OK You do not state what the elephant tissue is. If it is skin....maybe a problem....I have problems with human keloid skin samples. I am currently assessing Trajan 3 slides ( for adherence problems with SuperfrostPlus sides) As stated: no specie is any different. Well, yes, there are differences. For eg: worm Pwax sections are not as adherent as Ms/rat...etc. I do not know why Sigh Great forum! Carlos From Histology at nwlabs.co.uk Sat Sep 8 12:12:20 2018 From: Histology at nwlabs.co.uk (Histology) Date: Sat, 8 Sep 2018 17:12:20 +0000 Subject: [Histonet] Giemsa vs Diff Quik Message-ID: Hi, Have you tried leishman staining? I have an automated protocol for he thermo gemini if you want one? Failing that I do have the manual step protocol and all you need is a jar of leishman powder, methanol and pH6.8 phosphate buffet tablets Stuart Beaver BSc(Hons) Head of Veterinary Histology/Cytology +447568543761 On 6 Sep 2018, at 21:37, Bob Richmond > wrote: Joseph A. Esposito at McClain Laboratories on Long Island asks: The laboratory I work at has been using the Diff Quik for years now as a stain for fine needle aspirates. Recently, when we tried to reorder a Diff Quik stain kit from our usual suppliers, we have found it to be on backorder. This has caused us to begin to consider the Giemsa stain as an alternative to the Diff Quik. Does anyone have any experience with using both stains and how they compare to each other? Would the Giemsa be a suitable alternative to replace the Diff Quik?<< Diff-Quik? (spelt like that!) is an old registered trademark for a rapid two-step Romanowsky stain. A number of Web sites inform me today that this trade name has been discontinued, I suppose because the name has been so commonly used generically. There have long been a number of generic fast two-step Romanowsky stains available, with a xanthene dye (presumably eosin Y) as solution 1, and a proprietary mixture of thiazine dyes as solution 2. In my experience with at least five of these, they all worked pretty much indistinguishably from brand-name Diff-Quik. There are a number of stains called Giemsa, also Romanowsky stains, but often slower than the "quick" stains. There is probably no advantage to using them for any reason. In my eightieth year, I remember with nostalgia the old Wolbach Giemsa technique for tissue sections. Tissue must be fixed in a dichromate fixative, traditionally Zenker/Helly, sections stained in four successive Giemsa baths, the last one overnight, and differentiated with 10% colophonium rosin in alcohol with microscopic control. (I've actually done this stain myself.) Bob Richmond Samurai Pathologist Maryville TN From CDavis at che-east.org Mon Sep 10 12:57:30 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Mon, 10 Sep 2018 17:57:30 +0000 Subject: [Histonet] slide dryer temperature monitoring Message-ID: <4b5b3693da984c34b8dda794f522e2b9@che-east.org> Hello Histonet Folks, does anyone know of a way to monitor the actual operating temperature of the slide dryer on automated stainers? Our area is very humid in the small state and were are/were having trouble with the top sections (1st level) on our slides falling off. We are trying to determine weither it is a quality control issue with the charged slides or a issue with the operating temperature of the slide dryer on the automated stainer. The display monitor for our stainer does not have an area showing actual operating temperature and is is several screens to check the set temperature. (hey manufactors this would be an excellent upgrade) I've already bent our sales persons ear about this I am hoping someone out there has a doable work around. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From plucas at biopath.org Mon Sep 10 16:53:27 2018 From: plucas at biopath.org (Paula) Date: Mon, 10 Sep 2018 14:53:27 -0700 Subject: [Histonet] Pathologist Assistant- Part-time Message-ID: <004901d44950$b593ca40$20bb5ec0$@biopath.org> Hello, We are small private laboratory in Fountain Valley, California and we are looking for a licensed PA. If interested and if you have any questions, please contact Jason Cochran, Russel Blayney or Paula Lucas at 714-433-1330, or simply respond to this email. Thank you, Paula Lucas Lab Manager Bio-Path Medical Group Fountain Valley, CA From relia1 at earthlink.net Tue Sep 11 09:31:54 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 11 Sep 2018 10:31:54 -0400 Subject: [Histonet] Hope to See You in St. Louis at the NSH SC! And Here are some Exciting New Opportunities! Message-ID: <005801d449dc$2feb9cb0$8fc2d610$@earthlink.net> Hi Histonetters, How are you? As you know I do the majority of my work via email and phone so I rarely get a chance to meet people in person. That is one of the many reasons I enjoy attending the NSH conventions. I wanted to drop you a line and see if you or anyone you work with is planning on attending the NSH convention in St. Louis next week? If so I would love to have the opportunity to say Hi. I will be very easy to find because I am working the registration desk all day Friday, Saturday, Sunday and Monday so please if you are going to be there stop by and say Hi. Also if you haven?t already done so, check out the NSH convention app. It is available at the NSH convention Attendee website. It has some really cool features to enhance your convention experience. If you aren?t attending this year follow the fun on Facebook or on twitter we will be posting with the hashtag #nshsc one of my volunteer responsibilities this year is social networking so I will be posting lots of pics and statuses about all the exciting stuff going on so stay tuned!! If you are interested in a new opportunity I have some exciting H ? Virginia (Senior Tech) ? North Carolina (Management and Tech positions) ? SW Florida ? (Mohs Tech) ? California ? (Get off the Bench!) ? Chattanooga (IHC) ? Missouri ? KC ? Senior Tech All of these positions are full time and permanent and most of my clients offer relocation assistance and or a sign on bonus. For more info please contact me at relia1 at earthlink.net or on my cell/text at 407-353-5070 or toll free at the office at 866-607-3542. Hope to see you in St. Louis!! ? Have a great day!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Ana.Maluenda at baker.edu.au Tue Sep 11 18:53:20 2018 From: Ana.Maluenda at baker.edu.au (Ana Maluenda) Date: Tue, 11 Sep 2018 23:53:20 +0000 Subject: [Histonet] Platelet staining on FFPE mouse tissues Message-ID: Hi everyone, Does anyone has experience performing IHC staining for platelets on mouse FFPE? I've been trying for a while with different primary antibodies unsuccessfully. I found two that work in cryosections, but not a single hint of positivity on FFPE tissues. The samples have been prepared as usual (NBF fixation, graded EtOH, Xylene, wax...) and thin sections were collected by usual procedures (think they are 5 um). Unfortunately I have limited resources, so my protocol has been manual staining (no autostainer) and I have only microwave or water baths available around to play with Ag retrievals. I also already tried Sodium Citrate and Citrate buffers at pH = 6 and 9. Anyone has a protocol that works and that they would be willing to share? Much appreciated! Kind regards, Ana Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting privacy at baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg From PKRichar at gundersenhealth.org Wed Sep 12 08:33:34 2018 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Wed, 12 Sep 2018 13:33:34 +0000 Subject: [Histonet] slide dryer Message-ID: <998284C32F61104CA0BEFFFFCF6F90FDE51284DF@LXEXMB01.gundluth.org> Hi, does anyone use a slide drying oven? If so what brand and are the slides dry enough to file after they come out of the dryer? Cordially, Pam ~ National Histology Professionals Day 3/10/19 Pathologist Assistant Day 4/14/2019 Medical Laboratory Professionals Week April 21-27, 2019 National Cytotechnology Day 5/13/2019 +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org From Lisa.Chapman at aurora.org Wed Sep 12 14:18:11 2018 From: Lisa.Chapman at aurora.org (Chapman, Lisa) Date: Wed, 12 Sep 2018 19:18:11 +0000 Subject: [Histonet] Luxol fast Blue Question Message-ID: Hello All, When performing a LFB for myelin and nissl, how long does everyone keep their slides in the LFB solution for and what temp do you use? Most procedures say "overnight" and Sheehan states 16-24 hours. Any advice would be greatly appreciated! Thank you in advance! Lisa Chapman HT (ASCP) ACL Laboratories Milwaukee, WI From bcooper at chla.usc.edu Wed Sep 12 14:29:41 2018 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 12 Sep 2018 19:29:41 +0000 Subject: [Histonet] Luxol fast Blue Question In-Reply-To: References: Message-ID: <0f64a08df81b4edf8253127c0599c48a@chla.usc.edu> Hi Lisa, We keep them in solution at 56-60 degrees overnight, and typically start differentiation between the 15-20 hour range. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper at chla.usc.edu -----Original Message----- From: Chapman, Lisa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, September 12, 2018 12:18 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Luxol fast Blue Question (EXTERNAL EMAIL) Hello All, When performing a LFB for myelin and nissl, how long does everyone keep their slides in the LFB solution for and what temp do you use? Most procedures say "overnight" and Sheehan states 16-24 hours. Any advice would be greatly appreciated! Thank you in advance! Lisa Chapman HT (ASCP) ACL Laboratories Milwaukee, WI _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From melissa at alliedsearchpartners.com Thu Sep 13 12:25:36 2018 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Thu, 13 Sep 2018 17:25:36 +0000 Subject: [Histonet] Job Opening(s) Message-ID: Hello, I am currently working to source and recruit for the following positions and wanted to alert histoland. Please message me if interested in exploring further. Also, I will be exhibiting at booth #634 at NSH in St. Louis and presenting a workshop on histology hiring hot topics on Saturday so I can be reached there as well. Have a wonderful "almost" weekend. Microdissection Tech-NJ Histology Supervisor-FL Histotech-GA Histotech-TN Part Time Histotech-WI Histotech-Central PA Histotech-FL Melissa Owens, CHP (ASA) President, Laboratory Staffing, Allied Search Partners From sandra.cheasty at wisc.edu Fri Sep 14 15:34:19 2018 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Fri, 14 Sep 2018 20:34:19 +0000 Subject: [Histonet] Ultra Clean Diluent for IHC Message-ID: Hi all, It seems Fisher has stopped carrying the "Ultra Clean Diluent 125ml". (Item TA-125-UC). Can anyone recommend someone else who carries it, or a likely replacement? We use the Po-Link 2+Broad HRP detection kit. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From jkempf80 at uga.edu Mon Sep 17 06:42:16 2018 From: jkempf80 at uga.edu (Jennifer Kempf) Date: Mon, 17 Sep 2018 11:42:16 +0000 Subject: [Histonet] Ultra Clean Diluent for IHC In-Reply-To: References: Message-ID: We used to get almost all of our IHC reagents from DAKO, but when they fully merged with Agilent their prices went up exponentially. We changed to Biocare Medical and have found that almost all of their reagents work better than DAKO's did. Some of our antibodies had to be diluted out more, but that saves too so its kind of a win-win. Anyway, their background reducing diluent is the one that we use, but they do have several different versions and their rep can tell you which one is most like the one that you were using from Fisher. I'm not sure who the rep is up there, but I can give you contact info of the person I would recommend talking to if you like. Happy Monday!! Jennifer M Kempf Histology Laboratory Manager Department of Pathology College of Veterinary Medicine University of Georgia 501 D.W. Brooks Drive Athens, Georgia 30602 Office: 706-542-2218 Lab: 706-542-5828 jkempf80 at uga.edu -----Original Message----- From: Sandra Cheasty [mailto:sandra.cheasty at wisc.edu] Sent: Friday, September 14, 2018 4:34 PM To: Histonet (histonet at lists.utsouthwestern.edu) Subject: [Histonet] Ultra Clean Diluent for IHC Hi all, It seems Fisher has stopped carrying the "Ultra Clean Diluent 125ml". (Item TA-125-UC). Can anyone recommend someone else who carries it, or a likely replacement? We use the Po-Link 2+Broad HRP detection kit. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From histo at pathlab.us Mon Sep 17 07:53:03 2018 From: histo at pathlab.us (Histology) Date: Mon, 17 Sep 2018 12:53:03 +0000 Subject: [Histonet] table for cutting Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD8206FB45C0@vdc01.domain.local> Does anyone use a table to put their microtome and cut? I'm trying to find one and I want to make sure that it is stable enough for cutting. Thanks, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 From lblazek at digestivespecialists.com Mon Sep 17 09:13:17 2018 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Mon, 17 Sep 2018 10:13:17 -0400 Subject: [Histonet] Job opening Dayton Oh Message-ID: <5A2BD13465E061429D6455C8D6B40E392A866D3E02@IBMB7Exchange.digestivespecialists.com> We're looking for a stand out Histology Technician. This position is Monday - Friday day shift position. We are a growing company with a brand new facility. To be successful in this job you must be focused, methodical, precise and able to work collaboratively with a variety of groups. You must be Certified or eligible for Board of Certification (BOC) by the American Society of Clinical Pathologists (ASCP) and have a minimum of one year of experience as a Histology Technician with competency in the areas of embedding, microtomy and special stains and with a basic knowledge of immunohistochemistry. You must be able to work as a team that values open and honest communication, embraces challenge, innovation and continuous quality improvement, works hard, has fun and displays a can-do attitude. Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com From tbraud at holyredeemer.com Mon Sep 17 12:56:06 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 17 Sep 2018 17:56:06 +0000 Subject: [Histonet] Ultra Clean Diluent for IHC (Jennifer Kempf) Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF704BE@HRHEX02-HOS.holyredeemer.local> I agree with Jennifer - Just another recommendation for BioCare and their reagents. They are a super company to work with and have excellent quality and quality control on their reagents. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From CDavis at che-east.org Tue Sep 18 12:30:20 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Tue, 18 Sep 2018 17:30:20 +0000 Subject: [Histonet] histology lab temperature and humidity Message-ID: At one of my jobs we were discussing the way excess humidity can cause tissue to fall off slides even when they are charged or treated and may need to be in the slide dryer longer. Isn't there a CAP requirement requiring monitoring of the humidity as well as temperature in the actual lab not just where slides are stored? Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From christopher.hayden at novartis.com Tue Sep 18 12:35:56 2018 From: christopher.hayden at novartis.com (Hayden, Christopher) Date: Tue, 18 Sep 2018 17:35:56 +0000 Subject: [Histonet] table for cutting (Histology) Message-ID: Good Day, Mehndi: One of the colleges around here used this bench in a pinch: https://www.sears.com/craftsman-premium-heavy-duty-8-ft-workbench-with/p-00946638000P?plpSellerId=Sears&prdNo=5&blockNo=5&blockType=G5 I don't know if it's too large for your space, but it's certainly sturdy. Cheers, -CH Christopher Hayden T +1 862 778 7993 christopher.hayden at novartis.com -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Monday, September 17, 2018 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 178, Issue 14 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=6tODSDatJI3Ws5IvTkYGkN2v4q1-QaA07oSrqx825Ek&s=59v_l8A-vACB7Ap1Fh66zkMl9PpoQNFAX0W4ZHx5FPU&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Ultra Clean Diluent for IHC (Jennifer Kempf) 2. table for cutting (Histology) 3. Job opening Dayton Oh (Blazek, Linda) ---------------------------------------------------------------------- Message: 1 Date: Mon, 17 Sep 2018 11:42:16 +0000 From: Jennifer Kempf To: Sandra Cheasty , "Histonet (histonet at lists.utsouthwestern.edu)" Subject: Re: [Histonet] Ultra Clean Diluent for IHC Message-ID: Content-Type: text/plain; charset="us-ascii" We used to get almost all of our IHC reagents from DAKO, but when they fully merged with Agilent their prices went up exponentially. We changed to Biocare Medical and have found that almost all of their reagents work better than DAKO's did. Some of our antibodies had to be diluted out more, but that saves too so its kind of a win-win. Anyway, their background reducing diluent is the one that we use, but they do have several different versions and their rep can tell you which one is most like the one that you were using from Fisher. I'm not sure who the rep is up there, but I can give you contact info of the person I would recommend talking to if you like. Happy Monday!! Jennifer M Kempf Histology Laboratory Manager Department of Pathology College of Veterinary Medicine University of Georgia 501 D.W. Brooks Drive Athens, Georgia 30602 Office: 706-542-2218 Lab: 706-542-5828 jkempf80 at uga.edu -----Original Message----- From: Sandra Cheasty [mailto:sandra.cheasty at wisc.edu] Sent: Friday, September 14, 2018 4:34 PM To: Histonet (histonet at lists.utsouthwestern.edu) Subject: [Histonet] Ultra Clean Diluent for IHC Hi all, It seems Fisher has stopped carrying the "Ultra Clean Diluent 125ml". (Item TA-125-UC). Can anyone recommend someone else who carries it, or a likely replacement? We use the Po-Link 2+Broad HRP detection kit. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine ------------------------------ Message: 2 Date: Mon, 17 Sep 2018 12:53:03 +0000 From: Histology To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] table for cutting Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD8206FB45C0 at vdc01.domain.local> Content-Type: text/plain; charset="us-ascii" Does anyone use a table to put their microtome and cut? I'm trying to find one and I want to make sure that it is stable enough for cutting. Thanks, Mehndi Helgren Dominion Pathology Laboratories 733 Boush St. Suite 200 Norfolk, VA 23510 757-664-7901 ------------------------------ Message: 3 Date: Mon, 17 Sep 2018 10:13:17 -0400 From: "Blazek, Linda" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Job opening Dayton Oh Message-ID: <5A2BD13465E061429D6455C8D6B40E392A866D3E02 at IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" We're looking for a stand out Histology Technician. This position is Monday - Friday day shift position. We are a growing company with a brand new facility. To be successful in this job you must be focused, methodical, precise and able to work collaboratively with a variety of groups. You must be Certified or eligible for Board of Certification (BOC) by the American Society of Clinical Pathologists (ASCP) and have a minimum of one year of experience as a Histology Technician with competency in the areas of embedding, microtomy and special stains and with a basic knowledge of immunohistochemistry. You must be able to work as a team that values open and honest communication, embraces challenge, innovation and continuous quality improvement, works hard, has fun and displays a can-do attitude. Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=6tODSDatJI3Ws5IvTkYGkN2v4q1-QaA07oSrqx825Ek&s=59v_l8A-vACB7Ap1Fh66zkMl9PpoQNFAX0W4ZHx5FPU&e= ------------------------------ End of Histonet Digest, Vol 178, Issue 14 ***************************************** From Timothy.Morken at ucsf.edu Tue Sep 18 13:02:58 2018 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 18 Sep 2018 18:02:58 +0000 Subject: [Histonet] histology lab temperature and humidity In-Reply-To: References: Message-ID: Cassandra, the requirement for humidity covers requirements for equipment (specifications will specify operating conditions - temperature, humidity), chemicals (some are hygroscopic and high humidity can shorten shelf life) and "working environment." If you find that you need certain humidity levels for good results, or worker comfort, you can put that in your environmental procedures. The question is, what can you do about it to change the levels? Besides those things, low humidity is a fire danger, and continuous high humidity can be an infection control concern. We set our humidity range at 20% to 60%. Here we never get to 20% but we sometimes go over 60% in some our labs on mild foggy days, mainly in older buildings. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cassie P. Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 18, 2018 10:30 AM To: histonet Subject: [Histonet] histology lab temperature and humidity At one of my jobs we were discussing the way excess humidity can cause tissue to fall off slides even when they are charged or treated and may need to be in the slide dryer longer. Isn't there a CAP requirement requiring monitoring of the humidity as well as temperature in the actual lab not just where slides are stored? Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff at uropartners.com Tue Sep 18 15:39:32 2018 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 18 Sep 2018 20:39:32 +0000 Subject: [Histonet] Something to share with all the men in your laboratories and lives Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF11DCB536@COLOEXCH01.uropartners.local> Get PSA tested. It saves lives: http://www.chicagonow.com/downsize-maybe/2018/09/are-400000-men-enough-to-prove-a-psa-point/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From CDavis at che-east.org Wed Sep 19 09:06:10 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Wed, 19 Sep 2018 14:06:10 +0000 Subject: [Histonet] histology lab temperature and humidity In-Reply-To: References: , Message-ID: Thanks Tim, you're an excellent resource as always. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org ________________________________ From: Morken, Timothy Sent: Tuesday, September 18, 2018 2:02 PM To: Cassie P. Davis Cc: Histonet Subject: [External] RE: histology lab temperature and humidity Warning: This email originated from the Internet! DO NOT CLICK links if the sender is unknown, and NEVER provide your password. Cassandra, the requirement for humidity covers requirements for equipment (specifications will specify operating conditions - temperature, humidity), chemicals (some are hygroscopic and high humidity can shorten shelf life) and "working environment." If you find that you need certain humidity levels for good results, or worker comfort, you can put that in your environmental procedures. The question is, what can you do about it to change the levels? Besides those things, low humidity is a fire danger, and continuous high humidity can be an infection control concern. We set our humidity range at 20% to 60%. Here we never get to 20% but we sometimes go over 60% in some our labs on mild foggy days, mainly in older buildings. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Cassie P. Davis via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 18, 2018 10:30 AM To: histonet Subject: [Histonet] histology lab temperature and humidity At one of my jobs we were discussing the way excess humidity can cause tissue to fall off slides even when they are charged or treated and may need to be in the slide dryer longer. Isn't there a CAP requirement requiring monitoring of the humidity as well as temperature in the actual lab not just where slides are stored? Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From criley at dpspa.com Wed Sep 19 09:43:17 2018 From: criley at dpspa.com (Charles Riley) Date: Wed, 19 Sep 2018 10:43:17 -0400 Subject: [Histonet] braf (v6001e) Message-ID: Does anyone use this antibody on the Leica Bond systems? I have used several different vendors to validate this antibody but the Docs say all of them are over staining or not working correctly (I've gone down to 1:5000 on the dilution on all of them). If anyone has any suggestions on protocols or vendors that have worked for you (other than Ventana) please let me know. Thanks -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From mtoole at dcol.net Wed Sep 19 11:58:06 2018 From: mtoole at dcol.net (Mike Toole) Date: Wed, 19 Sep 2018 11:58:06 -0500 Subject: [Histonet] Cytology from formalin Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB358D847574A@mail> Even though this is off label I am interested in hearing input about producing a ThinPrep pap from cells collected in formalin. I suppose spinning it down and doing a Cytolyt wash followed by transfer to Preservcyt would work. Has anyone tried this or have insight into whether the fixation and pap staining would vary substantially from cells collected in alcohol. Thanks, Mike Toole, BS, CT(ASCP)CM From tim.higgins at cddmedical.com Wed Sep 19 13:06:20 2018 From: tim.higgins at cddmedical.com (Tim Higgins) Date: Wed, 19 Sep 2018 18:06:20 +0000 Subject: [Histonet] Subject: braf (v6001e) Message-ID: Hey Charles, I am sure you have thought about this but your retrieval process could be too stringent if you are having over expression. Thanks, Timothy Higgins, HT(ASCP), QIHC(ASCP)cm Histology Supervisor Center for Disease Detection/LabCorp Tim.higgins at cddmedical.com Message: 6 Date: Wed, 19 Sep 2018 10:43:17 -0400 From: Charles Riley To: Histo List Subject: [Histonet] braf (v6001e) Message-ID: Content-Type: text/plain; charset="UTF-8" Does anyone use this antibody on the Leica Bond systems? I have used several different vendors to validate this antibody but the Docs say all of them are over staining or not working correctly (I've gone down to 1:5000 on the dilution on all of them). If anyone has any suggestions on protocols or vendors that have worked for you (other than Ventana) please let me know. Thanks -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From greg.dobbin at gmail.com Wed Sep 19 13:12:47 2018 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Wed, 19 Sep 2018 15:12:47 -0300 Subject: [Histonet] Braf protocol on Bond-III Message-ID: 1:100 with ER-2 for 30 mins. Clone is the V600E from Spring BioScience Looks great for uis and we are performing well on EQA surveys. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From mssabater19 at gmail.com Wed Sep 19 16:46:22 2018 From: mssabater19 at gmail.com (Matthew Sabater) Date: Wed, 19 Sep 2018 14:46:22 -0700 Subject: [Histonet] Career Opportunity: Providence Portland IHC Histotech 3 M-Fri 6am-2:30pm References: <3E3513BEBCF85444A4F3CFE6FCE3F14AFE5D95@WN24244.or.providence.org> Message-ID: <9280F049-31F1-4042-A297-03528D2EF221@gmail.com> > > > Hello Histonet, > > I currently have an open Histotech 3 position whose primary responsibility will be performing IHCs. We are looking for a motivated, adaptable, and technically sound histotech that thrives in a fast-paced team environment. > > Providence Health and Services: Full-time, Monday-Friday 6:00am-2:30pm Location: Portland, OR > > In this position you will: > Be responsible for the accurate and timely preparation of material for study and diagnostic interpretation by pathologists. > Perform pre-analytical, analytical and post-analytical duties including tissue processing, routine stains, special stains, immunohistochemical stains, instrument maintenance and problem resolution. > Work with acute-care and outpatient caregivers, vendors, consultants, and other Laboratory Services staff in performing their duties. > Advance through the four defined stages of technical development within this job category (Participatory, Self-directed, Leadership and Expert). There are four (4) major areas of activity within each stage: 1) Education, Training, and Experience; 2) Technical Skills; 3) Communication and Interpersonal Skills; 4) Business Knowledge. > Attain increased education, skills, knowledge and abilities and must demonstrate an ability to assume the increased responsibilities and accountabilities of the next stage as designated in this Job Category Description and on the Job Title Specification Sheet. > Be expected to possess an understanding of the Providence Health System and Laboratory Services goals and strategies. > Required qualifications for this position include: > Certification or Registry (HT, HTL) from a government agency or nationally recognized professional organization (eg. ASCP, NCA, etc.) > For HT, HTL with Bachelors degree: 5-10 years of industry related experience > For HT from accredited training program or OJT: 6-11 years of industry related experience > **Industry related at Level 3 requires that the experience be from an equivalent high volume or acute care laboratory performing high complexity testing > > Preferred qualifications for this position include: > Qualification in Immunohistochemistry, QIHC > If interested please send your resume to matthew.sabater at providence.org and apply online at https://www.providenceiscalling.jobs/ JOB ID# 200786 > > Thank You, > > Matthew Sabater > matthew.sabater at providence.org | Regional Histology Supervisor > Providence Health and Services | Oregon Regional Laboratory > Dept. line: 503-893-7736 Cell Phone: 503-539-4376 > > > > This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. From langston at hawaii.edu Wed Sep 19 23:10:59 2018 From: langston at hawaii.edu (Ross Langston) Date: Wed, 19 Sep 2018 18:10:59 -1000 Subject: [Histonet] Looking for manual and block holder for Sorval JB4 Microtome Message-ID: Hi, All. I am looking for a block holder (or ideas for suitable substitution) and owners manual for same. I have a lot of experience on the MT1, but recently came into a couple JB4s that I would like to get running. Any advice? Thanks much! Ross Ross Langston, PhD Assistant Professor of Zoology Windward Community College From criley at dpspa.com Thu Sep 20 09:45:12 2018 From: criley at dpspa.com (Charles Riley) Date: Thu, 20 Sep 2018 10:45:12 -0400 Subject: [Histonet] Cellblock H&E Message-ID: My pathologists are complaining that our cell blocks appear light on hematoxylin. Does anyone use a different protocol for their routine H&E vs. Cellblocks? If so what is the difference in protocols? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From PKRomund at gundersenhealth.org Thu Sep 20 10:00:20 2018 From: PKRomund at gundersenhealth.org (Romundstad, Pamela K) Date: Thu, 20 Sep 2018 15:00:20 +0000 Subject: [Histonet] tissue processing baskets for the Sakura-small 50 cassettes basket Message-ID: Hello, Does anyone have any tissue processing baskets for the Sakura-small 50 cassettes basket size that they no longer need and want to get rid of? If so, I'd be happpy to put them to good use. Thanks. Respectfully, Pamela Romundstad HT (ASCP), QIHCCM Lead Histology Technician Gundersen Health System H04-008 608-775-3139 From jaylundgren at gmail.com Thu Sep 20 10:04:49 2018 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 20 Sep 2018 11:04:49 -0400 Subject: [Histonet] Cellblock H&E In-Reply-To: References: Message-ID: Just a guess, but maybe increased time in hematoxylin? :) On Thu, Sep 20, 2018 at 10:58 AM Charles Riley via Histonet < histonet at lists.utsouthwestern.edu> wrote: > My pathologists are complaining that our cell blocks appear light on > hematoxylin. > > Does anyone use a different protocol for their routine H&E vs. Cellblocks? > If so what is the difference in protocols? > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Histology at nwlabs.co.uk Thu Sep 20 12:06:25 2018 From: Histology at nwlabs.co.uk (Histology) Date: Thu, 20 Sep 2018 17:06:25 +0000 Subject: [Histonet] Leica SCN400 slide carriers Message-ID: <8AEE3B1C-A672-41D0-9819-293772A49403@nwlabs.co.uk> Hi Histonet, I would like to ask if anyone has (or knows who may have) any spare slide carriers for the Leica SCN400 scanning microscope. Is anyone currently using this model and how does it perform? Best wishes Stuart Beaver BSc(Hons) Head of Veterinary Histology/Cytology +447568543761 From CDavis at che-east.org Fri Sep 21 07:17:54 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Fri, 21 Sep 2018 12:17:54 +0000 Subject: [Histonet] cellblocks Message-ID: All our tissue is stained with the same H&E protocol. The difference maybe because cellblock are processed different to turn a fluid into a cellblock rather sampling a section of tissue. Most places fix tissue in NBF. Are using ThinPrep processing? If I recall correctly, the specimens are 1st processed in cytolyt solution which I believe is a buffered alcohol solution before the are spun down for the cellblock button and put in a cassette with the tissues in NBF for processing. At least that is how I remember doing it when I did cytoprep +/-12 years ago. This might account for the difference in staining even when the cellblock section is the same thickness as a tissue section. I cannot speak for SurePath or other cytology processing. If this is a new issue, is there a change in the way the specimen is being processed, are different people cutting cellblocks then tissues, it could be a microtomy section thickness issue. Section cut thinner appear to stain lighter, I drove a patholgist crazy ealier in my early career because I cut at 4 microns and she was used to looking at sections 5-6 microns thick. I hope this helps. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From MDiCarlo at KaleidaHealth.Org Mon Sep 24 13:01:04 2018 From: MDiCarlo at KaleidaHealth.Org (DiCarlo, Margaret) Date: Mon, 24 Sep 2018 18:01:04 +0000 Subject: [Histonet] cleaning fluid & lubricating fluid discontinued Message-ID: <731BE09CDB19AA43AA8682199D42D31B011798E736@ADCEXCHANGE01.KaleidaHealth.org> Histonetters, Fisher-Scientific has discontinued selling Microsharp Cleaning Fluid and Microsharp Lubricating Fluid that I use for sharpening stainless steel knives on my Shandon Autosharp 5 knife sharpener. Can anyone recommend a comparable substitute that I can use in their place? Vendor's recommendations would also greatly be appreciated. Thank you in advance. Peggy DiCarlo HT (ASCP) Ortho Bone Lab Buffalo General Medical Center 100 High St. Buffalo, NY 14203 716-859-1293 [Leading with CARE] The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi ________________________________ CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida Health's Technology Assistance Center at (716) 859-7777. From emartinez2 at echd.org Mon Sep 24 14:16:36 2018 From: emartinez2 at echd.org (Estela Martinez) Date: Mon, 24 Sep 2018 19:16:36 +0000 Subject: [Histonet] ANP.12350 Cancer Protocols Message-ID: Hello Everyone, Just a quick question. I know that for the ANP.12350 Cancer Protocols, we have to have random samples of at least 10% of the eligible surgical pathology reports, or a total of 150 cases per year, as CAP requires it, but do we have to have a different percent for non cancer cases? Does anyone know and if you do, what percent and where can I find that in writing? Thank you so much!!! Estela Martinez Histology Supervisor Medical Center Hospital Odessa, TX 79761 432-640-2348 emartinez2 at echd.org CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party without permission of original user and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. From PKRichar at gundersenhealth.org Thu Sep 27 10:14:45 2018 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Thu, 27 Sep 2018 15:14:45 +0000 Subject: [Histonet] Glass wash question Message-ID: <998284C32F61104CA0BEFFFFCF6F90FDE514F061@LXEXMB01.gundluth.org> I am curious as to how you manage washing your glass ware. Are you using a dishwasher? Washing by hand? Sending to CS? If you wash by hand or use a dishwasher do you use DI water? Do you use contrad 70? Do you use mostly disposable? I appreciate any feedback. Cordially, Pam ~ National Histology Professionals Day 3/10/19 Pathologist Assistant Day 4/14/2019 Medical Laboratory Professionals Week April 21-27, 2019 National Cytotechnology Day 5/13/2019 +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org From kristyn.ferber at gmail.com Thu Sep 27 12:09:35 2018 From: kristyn.ferber at gmail.com (Kristyn Ferber) Date: Thu, 27 Sep 2018 13:09:35 -0400 Subject: [Histonet] Experience (especially long term) Message-ID: Hello Histonetters! I'm looking to gather histotech's experience with Thermo Scientific's HistoStar embedding workstation. I am very familiar with Sakura and think it's top notch as far as service and support and, especially, longevity. How does everyone feel the HistoStar compares with the Tissue Tek system if you've used both? Thanks in advance! Kristyn Ferber, HTL From bcooper at chla.usc.edu Thu Sep 27 12:31:33 2018 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Thu, 27 Sep 2018 17:31:33 +0000 Subject: [Histonet] Experience (especially long term) In-Reply-To: References: Message-ID: <564f1fc1d3994d15825efc13e5ed3e23@chla.usc.edu> I've used both. Agree that the Sakura's are awesome; used them for years at another institution. We currently have two of the Histostars, and we actually like the design a little better. The Histostar's hot plates are set back a little further than those on the Sakura model. In my case, this results in much fewer instances of "paraffin sleeves" on my lab coat! The remaining plastic space in front of the hot plate is a great place to align GI biopsies in your mold during orientation, or to flatten out and scrape paper wrapped cell block material. On our Histostar models (probably about 5 years old now) we don't have the ability to adjust the temperature of the cold plate. I do miss that, because we've found ours too be a little too cold, and we sometimes get cracks in our paraffin blocks (this is REALLY inconvenient on needle biopsies). I believe that on newer models, this feature has been added. That's my two cents worth... Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper at chla.usc.edu -----Original Message----- From: Kristyn Ferber via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, September 27, 2018 10:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Experience (especially long term) (EXTERNAL EMAIL) Hello Histonetters! I'm looking to gather histotech's experience with Thermo Scientific's HistoStar embedding workstation. I am very familiar with Sakura and think it's top notch as far as service and support and, especially, longevity. How does everyone feel the HistoStar compares with the Tissue Tek system if you've used both? Thanks in advance! Kristyn Ferber, HTL _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From tbraud at holyredeemer.com Thu Sep 27 12:34:51 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 27 Sep 2018 17:34:51 +0000 Subject: [Histonet] dishwasing Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF7DD09@HRHEX02-HOS.holyredeemer.local> We wash by hand, but do use some disposables (pipets, etc). We make one final DI wash, then check the pH for residual detergent. Record pH per batch washed. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Thursday, September 27, 2018 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 178, Issue 20 CAUTION : External Email Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Glass wash question (Richardson, Pam K) ---------------------------------------------------------------------- Message: 1 Date: Thu, 27 Sep 2018 15:14:45 +0000 From: "Richardson, Pam K" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Glass wash question Message-ID: <998284C32F61104CA0BEFFFFCF6F90FDE514F061 at LXEXMB01.gundluth.org> Content-Type: text/plain; charset="us-ascii" I am curious as to how you manage washing your glass ware. Are you using a dishwasher? Washing by hand? Sending to CS? If you wash by hand or use a dishwasher do you use DI water? Do you use contrad 70? Do you use mostly disposable? I appreciate any feedback. Cordially, Pam ~ National Histology Professionals Day 3/10/19 Pathologist Assistant Day 4/14/2019 Medical Laboratory Professionals Week April 21-27, 2019 National Cytotechnology Day 5/13/2019 +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 178, Issue 20 ***************************************** From sandra.cheasty at wisc.edu Thu Sep 27 13:40:14 2018 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Thu, 27 Sep 2018 18:40:14 +0000 Subject: [Histonet] CD204 Anti-MSR1 monoclonal, clone SRA-E5 Message-ID: Hello all, Does anyone know where I can get CD204, Anti-MSR1 monoclonal, clone SRA-E5? Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From krapp at rchsd.org Thu Sep 27 13:49:45 2018 From: krapp at rchsd.org (Rapp, Keith) Date: Thu, 27 Sep 2018 18:49:45 +0000 Subject: [Histonet] Cat Scratch Message-ID: <0c9a0302d3ed4c29b731e4236f33da5f@XCHPAPV09.RCHSD.org> Hello, can anyone recommend a vendor for bartonella henselae (Cat Scratch) IHC antibody? I have run across several vendors which have recently discontinued supplying it. Thank you, Keith Rapp Histology Technical Specialist Rady Children's Hospital Department of Pathology 858-966-1700 x5041 From shive003 at umn.edu Thu Sep 27 17:07:13 2018 From: shive003 at umn.edu (Jan Shivers) Date: Thu, 27 Sep 2018 17:07:13 -0500 Subject: [Histonet] CD204 Anti-MSR1 monoclonal, clone SRA-E5 In-Reply-To: References: Message-ID: Hi Sandy, I get mine from CosmoBio, catalog # KAL-KT022. Needs heat retrieval and I go 1 hr incubation in primary Ab at room temp. Good luck, Jan Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive003 at umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* On Thu, Sep 27, 2018 at 1:40 PM Sandra Cheasty via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello all, > Does anyone know where I can get CD204, Anti-MSR1 > monoclonal, clone SRA-E5? > Thanks! > Sandy > > Sandra J. Cheasty, HT (ASCP) > Histology & Necropsy Supervisor > UW-Madison, School of Veterinary Medicine > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From RCazares at schosp.org Fri Sep 28 11:07:42 2018 From: RCazares at schosp.org (Cazares, Ruth) Date: Fri, 28 Sep 2018 11:07:42 -0500 Subject: [Histonet] retracting or non-retracting microtome? Message-ID: Good morning all, Which is the best rotary microtome for routine tissues and biopsies? No plastic only paraffin. I am leaning toward retracting, but is there a benefit to non-retracting microtomes? Thanks in advance:) Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 N. California Chicago, IL 60625 (773) 878-8200 ext-5190