[Histonet] Clear-Rite 3 dirty slides

[Allan Wang]

Hi histonet,

For coverslipping Ventana IHC slides I have dish soap, rinse, alcohol 80%,
100%, 100%, 100%, followed by three Clear-Rite 3 instead of xylene. In the
past I didn't have this issue, but now I frequently get a lot of residue on
the slides after they enter the Clear-Rite.

If I completely dump all 3 containers of Clear-Rite 3 it gets rid of the
problem but I'd have to do this every month which is wasteful. If I just
dump the first container and move everything up, the problem still remains.

Images:
https://i.imgur.com/GTQJe2W.jpg
https://i.imgur.com/np8B5P2.jpg
https://i.imgur.com/SLypUAy.jpg

Anyone know what this contamination is and the best way to reduce it?

Allan