From Valerie.Hannen at parrishmed.com Mon Oct 1 05:08:39 2018 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Mon, 1 Oct 2018 06:08:39 -0400 Subject: [Histonet] GLASS HONING PLATES USED TO SHARPEN STEEL MICROTOME BLADES Message-ID: <450B7A81EDA0C54E97C53D60F00776C32432B7F6CF@isexstore03> Hi everyone, I am hoping someone out there in Histoland can help me. I am trying to find a company who still sells the glass honing plates used with the Leica/ Jung knife ( steel blades) sharpener. Thank you in advance, Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From tmcampbe at fmh.org Tue Oct 2 07:03:01 2018 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Tue, 2 Oct 2018 12:03:01 +0000 Subject: [Histonet] DI Water Message-ID: I am looking to install a small DI system or reverse osmosis system in my small GI lab. We are bringing IHC in house and I thought it would be cheaper and easier in the long run to have a DI system. I am not sure what type to get though. There are so many out there. I don't want anything too expensive. Any reccomendations?? Thanks! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 CONFIDENTIALITY NOTICE: This electronic mail transmission and any accompanying data files is confidential and is intended exclusively for the individual or entity to which it is addressed. The communication may contain information that is proprietary, privileged or confidential or otherwise legally exempt from disclosure. If you are not the named addressee or you otherwise have received this message in error, you are not authorized to read, print, copy or disseminate this message or any part of it. If you have received this message in error, please notify the sender immediately by email and delete all copies of this message. Receipt by anyone other than the named addressee is not a waiver of any attorney-client work product or other applicable privilege. From CBird at amli-denton.com Tue Oct 2 07:30:34 2018 From: CBird at amli-denton.com (Cindy Bird) Date: Tue, 2 Oct 2018 07:30:34 -0500 Subject: [Histonet] (no subject) Message-ID: Can anyone out in histo land give me feedback on the IHC instrument by StatLab Quantum HDX and how it compares to other automated platforms. Thanks in advance! Cindy Bird Anatomical Medical Laboratories, Inc. 1600 Scripture Street Denton, TX 76201 940-384-6210 940-384-6000 Fax 940-565-9588 From relia1 at earthlink.net Tue Oct 2 11:02:28 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 2 Oct 2018 12:02:28 -0400 Subject: [Histonet] The NSH/SC in St. Louis was great!!! Message-ID: <000001d45a69$51893740$f49ba5c0$@earthlink.net> Hello Histonetters! How are you doing? I had such a great time at the NSHSC in St. Louis, MO !! I enjoyed making new friends, reconnecting with old friends and learning new things. I worked the registration desk with some really fun histotechs! I posted some pictures on Facebook Instagram and Twitter. If you get a chance check them out!! I really encourage everyone to go to meetings when they can. If you can?t make the national meeting try to make it to a regional or state meeting. The camaraderie and educational opportunities are phenomenal. Next year the convention will be held in New Orleans!! Hope to see you there! ? My phone never stopped ringing while I was gone!! ? All of my positions are full time permanent positions. ? My clients offer excellent compensation, benefits and relocation assistance. **And they are ready to interview and hire right away!** Here Is A List Of My Current Openings: HISTOLOGY SUPERVISORS/MANAGERS: Fayetteville, NC ? Histology Lab Manager Atlanta, GA ? Dermpath Histology Lab Manager HISTOTECHNICIANS/HISTOTECHNOLOGISTS: Grossing Histotech ? San Diego ? DAYS!! Histotechnician ? Norfolk, VA DAYS!!/sign on bonus!! Histotechnician ? Hammond, IN Histotech ?Fayetteville, NC ? DAYS!! Histotech- Dallas, TX Histotech ? Tallahassee, FL Mohs Tech ? Fayetteville, AR Grossing Histotech ? Chattanooga, TN Histotech ? Kansas City, MO Of course I can?t put all the information about these opportunities in an e-mail. So if you or anyone you know might be interested in hearing more about any of these positions or want help with a job search in another area please contact me. I can be reached at 866-607-3542 or on my cell at 407-353-5070 or relia1 at earthlink.net. Remember it never hurts to look. Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From caithesketh at gmail.com Wed Oct 3 08:20:58 2018 From: caithesketh at gmail.com (Caitlin Hesketh) Date: Wed, 3 Oct 2018 09:20:58 -0400 Subject: [Histonet] slide folders with space for blocks too Message-ID: I want to order some folders that have space for blocks to go with the slides. I swear this exists - I remember very clearly bringing it up as a suggestion at my last job and the pathologist there shutting it down instantly (he didn't want to be responsible for checking the block against the slide himself). The only ones I can find googling now are these: http://sourcemp.com/consulting-folder-for-slides-and-blocks.aspx But I am certain I saw them before with room for 20, and they were plastic if I remember right. Anyone else remember these things, or have I lost my mind? From Reuel.Cornelia at tsrh.org Wed Oct 3 10:26:25 2018 From: Reuel.Cornelia at tsrh.org (Reuel Cornelia) Date: Wed, 3 Oct 2018 15:26:25 +0000 Subject: [Histonet] Oil Red O Message-ID: I was wondering if you could help me know why our Oil Red O have some black snowflakes on our fat tissue hours after staining or after 24 hours more snowlflakes precipitation occurs. The staining was reference was from Lillie RD, Ashburn. Please note that our staining works well between two to three hours. Is there a reason fro this precipitation? Reuel TSRH Dallas, TX ---------------------------------------------------------------------- Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. From mills at 3scan.com Wed Oct 3 11:17:57 2018 From: mills at 3scan.com (Caroline Miller) Date: Wed, 3 Oct 2018 09:17:57 -0700 Subject: [Histonet] Oil Red O In-Reply-To: References: Message-ID: Hi Reuel, In my experience this is the ORO crystallizing out on the section. I would first filter your staining solution, then keep your staining times to as short as you can. Also changing between 37 and room temperatures may be useful too. mills ? On Wed, Oct 3, 2018 at 8:39 AM Reuel Cornelia via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I was wondering if you could help me know why our Oil Red O have some > black snowflakes on our fat tissue hours after staining or after 24 hours > more snowlflakes precipitation occurs. The staining was reference was from > Lillie RD, Ashburn. Please note that our staining works well between two to > three hours. Is there a reason fro this precipitation? > > > Reuel > > TSRH > > Dallas, TX > > ---------------------------------------------------------------------- > Texas Scottish Rite Hospital for Children is one of the nation's leading > pediatric centers for the treatment of orthopedic conditions, certain > related neurological disorders and learning disorders, such as dyslexia. > This email transmission and/or its attachments may contain confidential > health information, intended only for the use of the individual or entity > named above. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Biology www.3Scan.com 415 2187297 From amosbrooks at gmail.com Wed Oct 3 17:25:09 2018 From: amosbrooks at gmail.com (Amos Brooks) Date: Wed, 3 Oct 2018 18:25:09 -0400 Subject: [Histonet] Oil Red O Message-ID: Hi, I have seen this too. I mitigate the problem by making it up in 50 ml increments and staining them flat. I draw from around the middle of the Falcon tube I make it up in. It tends to precipitate so you could filter it. Don't bother re-using the reagent. It's cheap & easy to make up. I usually only see the precipitate with old solutions. Just make what you use within a few weeks. Best 'o luck, Amos > > Message: 2 > Date: Wed, 3 Oct 2018 15:26:25 +0000 > From: Reuel Cornelia > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Oil Red O > Message-ID: > < > SN1PR17MB0543F87DBC5E39AA8875C9F1E7E90 at SN1PR17MB0543.namprd17.prod.outlook.com > > > > Content-Type: text/plain; charset="iso-8859-1" > > I was wondering if you could help me know why our Oil Red O have some > black snowflakes on our fat tissue hours after staining or after 24 hours > more snowlflakes precipitation occurs. The staining was reference was from > Lillie RD, Ashburn. Please note that our staining works well between two to > three hours. Is there a reason fro this precipitation? > > > Reuel > > TSRH > > Dallas, TX > > From adesupo2002 at hotmail.com Wed Oct 3 21:49:55 2018 From: adesupo2002 at hotmail.com (ADESUPO ADESUYI) Date: Thu, 4 Oct 2018 02:49:55 +0000 Subject: [Histonet] Annual Competency Message-ID: Hi, I am in the process of updating the competencies for histotechs and lab Assistant in our lab. I will appreciate it, if you guys could share. Thanks, Ade From adesupo2002 at hotmail.com Wed Oct 3 21:53:58 2018 From: adesupo2002 at hotmail.com (ADESUPO ADESUYI) Date: Thu, 4 Oct 2018 02:53:58 +0000 Subject: [Histonet] AFB STAIN Message-ID: Hello, I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide? Thanks, Ade From tony.henwood at health.nsw.gov.au Wed Oct 3 23:35:20 2018 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 4 Oct 2018 04:35:20 +0000 Subject: [Histonet] AFB STAIN In-Reply-To: References: Message-ID: <2abe15ddaa9b4f0c9164e5baee47aad3@SVDCMBX-MEX024.nswhealth.net> We do, It works well Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: ADESUPO ADESUYI via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 4 October 2018 12:54 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] AFB STAIN Hello, I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide? Thanks, Ade _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From tmcampbe at fmh.org Thu Oct 4 07:34:44 2018 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Thu, 4 Oct 2018 12:34:44 +0000 Subject: [Histonet] Control Blocks Message-ID: Does anyone happen to have or know where to get HSV I and II and CMV control blocks? The control slides are so expensive and it would be so much easier to have a block that will last a long time and give me many for slides. Thanks!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 CONFIDENTIALITY NOTICE: This electronic mail transmission and any accompanying data files is confidential and is intended exclusively for the individual or entity to which it is addressed. The communication may contain information that is proprietary, privileged or confidential or otherwise legally exempt from disclosure. If you are not the named addressee or you otherwise have received this message in error, you are not authorized to read, print, copy or disseminate this message or any part of it. If you have received this message in error, please notify the sender immediately by email and delete all copies of this message. Receipt by anyone other than the named addressee is not a waiver of any attorney-client work product or other applicable privilege. From cls71877 at gmail.com Thu Oct 4 07:35:02 2018 From: cls71877 at gmail.com (Cristi Rigazio) Date: Thu, 4 Oct 2018 08:35:02 -0400 Subject: [Histonet] AFB STAIN In-Reply-To: <2abe15ddaa9b4f0c9164e5baee47aad3@SVDCMBX-MEX024.nswhealth.net> References: <2abe15ddaa9b4f0c9164e5baee47aad3@SVDCMBX-MEX024.nswhealth.net> Message-ID: <13BBFCF2-9B4F-43A3-9DCD-D1EB9FE60B0A@gmail.com> We always try to put them in the same slide, but sometimes it doesn?t work out the way. Either would be acceptable. Sent from my iPhone > On Oct 4, 2018, at 12:35 AM, Tony Henwood (SCHN) via Histonet wrote: > > We do, > > It works well > > > Regards > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) > Principal Scientist, the Children's Hospital at Westmead > Adjunct Fellow, School of Medicine, University of Western Sydney > Tel: 612 9845 3306 > Fax: 612 9845 3318 > Pathology Department > the children's hospital at westmead > Cnr Hawkesbury Road and Hainsworth Street, Westmead > Locked Bag 4001, Westmead NSW 2145, AUSTRALIA > > > -----Original Message----- > From: ADESUPO ADESUYI via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, 4 October 2018 12:54 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] AFB STAIN > > Hello, > I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide? > > Thanks, > Ade > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From boznpl at aol.com Thu Oct 4 07:36:53 2018 From: boznpl at aol.com (Laurie Colbert) Date: Thu, 4 Oct 2018 08:36:53 -0400 Subject: [Histonet] AFB STAIN In-Reply-To: References: Message-ID: <1663f14b54f-1ec0-1da@webjasstg-vab26.srv.aolmail.net> You should put the AFB control on a separate slide to prevent cross-contamination.? Laurie Redmond -----Original Message----- From: ADESUPO ADESUYI via Histonet To: histonet Sent: Wed, Oct 3, 2018 7:58 pm Subject: [Histonet] AFB STAIN Hello, I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide? Thanks, Ade _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From funderwood at mcohio.org Thu Oct 4 07:58:43 2018 From: funderwood at mcohio.org (Underwood, Fred) Date: Thu, 4 Oct 2018 12:58:43 +0000 Subject: [Histonet] microtome woes Message-ID: <7d6d9d40255b4483b86c90a647c7659c@Exch2013-mb01.mcohio.org> Greetings to all, I have a microtome, purchased in 2013, that now needs both circuit boards replaced. For the low, low price of $6400. This seems like it may not be far off from the price of a new unit. Would some of you be willing to share what brand of microtome you have, and what you paid? I am hesitant to share on a public forum what my unit is, but privately.... Thanks for your input, Fred From leonard at rrclinicallab.com Thu Oct 4 15:04:23 2018 From: leonard at rrclinicallab.com (leonard at rrclinicallab.com) Date: Thu, 04 Oct 2018 15:04:23 -0500 Subject: [Histonet] CMV Message-ID: <101a42f31eed70a197af8716a00f2247@rrclinicallab.com> Hi Tasha. My name is Leonard.I work at Macneal Hospital in Illinois.We currently have CMV blocks,and are will to trade for AFB material. Please let me know if this works for you. Leonard Ringo Lead Technician Macneal Hospital Berwyn Il. 708.783.5124 From tony.henwood at health.nsw.gov.au Thu Oct 4 15:47:35 2018 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 4 Oct 2018 20:47:35 +0000 Subject: [Histonet] AFB STAIN In-Reply-To: <1663f14b54f-1ec0-1da@webjasstg-vab26.srv.aolmail.net> References: , <1663f14b54f-1ec0-1da@webjasstg-vab26.srv.aolmail.net> Message-ID: <1538686057699.70997@health.nsw.gov.au> Cross-contamination has not been proven in FFPE tissues. I have not seen it in nearly 40 years of practice Do you have any evidence of cross-contamination in FFPE? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Laurie Colbert via Histonet Sent: Thursday, October 4, 2018 10:36 PM To: adesupo2002 at hotmail.com; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] AFB STAIN You should put the AFB control on a separate slide to prevent cross-contamination. Laurie Redmond -----Original Message----- From: ADESUPO ADESUYI via Histonet To: histonet Sent: Wed, Oct 3, 2018 7:58 pm Subject: [Histonet] AFB STAIN Hello, I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide? Thanks, Ade _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From katherine at ka-recruiting.com Thu Oct 4 16:07:01 2018 From: katherine at ka-recruiting.com (Katherine Marano) Date: Thu, 4 Oct 2018 17:07:01 -0400 Subject: [Histonet] histology manager needed Message-ID: Hi Histonetters! I hope you are having a great week. I just got in a brand new opening with a client of mine in New Jersey. They are looking to hire a permanent and full-time Histology Manager. There will be some Saturdays required with the position. Also, it is very important that candidates have supervisory or managerial experience in Histology. If you are interested could you send me a resume and a good time to give you a quick phone call? I'd love to tell you more. Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From wdesalvo.cac at outlook.com Thu Oct 4 16:26:58 2018 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Thu, 4 Oct 2018 21:26:58 +0000 Subject: [Histonet] AFB STAIN In-Reply-To: <1538686057699.70997@health.nsw.gov.au> References: , <1663f14b54f-1ec0-1da@webjasstg-vab26.srv.aolmail.net>, <1538686057699.70997@health.nsw.gov.au> Message-ID: I completely agree with Tony. Not likely to see any sloughing off (shedding) of organisms from FFPE tissue blocks. William DeSalvo ________________________________ From: Tony Henwood (SCHN) via Histonet Sent: Thursday, October 4, 2018 1:54 PM To: adesupo2002 at hotmail.com; Laurie Colbert Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] AFB STAIN Cross-contamination has not been proven in FFPE tissues. I have not seen it in nearly 40 years of practice Do you have any evidence of cross-contamination in FFPE? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________________ From: Laurie Colbert via Histonet Sent: Thursday, October 4, 2018 10:36 PM To: adesupo2002 at hotmail.com; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] AFB STAIN You should put the AFB control on a separate slide to prevent cross-contamination. Laurie Redmond -----Original Message----- From: ADESUPO ADESUYI via Histonet To: histonet Sent: Wed, Oct 3, 2018 7:58 pm Subject: [Histonet] AFB STAIN Hello, I have a question please. For the AFB Stain, do we put both the control tissue and the patient on the same slide? Thanks, Ade _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7C%7Cfe0f25b67e0d4783c39008d62a3b84a1%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636742832440684556&sdata=WQS0%2F1lqe%2FRJ0mB6k4NNlqYdIbvQ5sPx2EVn213ittE%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7C%7Cfe0f25b67e0d4783c39008d62a3b84a1%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636742832440684556&sdata=WQS0%2F1lqe%2FRJ0mB6k4NNlqYdIbvQ5sPx2EVn213ittE%3D&reserved=0 This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7C%7Cfe0f25b67e0d4783c39008d62a3b84a1%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636742832440684556&sdata=WQS0%2F1lqe%2FRJ0mB6k4NNlqYdIbvQ5sPx2EVn213ittE%3D&reserved=0 From m.jamison at elitechgroup.com Thu Oct 4 16:45:56 2018 From: m.jamison at elitechgroup.com (Michelle Jamison) Date: Thu, 4 Oct 2018 15:45:56 -0600 Subject: [Histonet] cytology Message-ID: <54ec41bc-7cdb-4921-595e-aa302c3f25d7@elitechgroup.com> Hi All, Do you know of a similar list serve that is for Cytologists? Thank you, Michelle -- All the best to you, Michelle Jamison /Bio Research Associate / /ELITechGroup Inc. / ???370 W. 1700 S.?? ???Logan Utah ??? USA m.jamison at elitechgroup.com ? _www.elitechgroup.com _ Tel : +1.435.752.6011 Ext: 1474 Logo From mtoole at dcol.net Thu Oct 4 17:43:34 2018 From: mtoole at dcol.net (Mike Toole) Date: Thu, 4 Oct 2018 17:43:34 -0500 Subject: [Histonet] cytology In-Reply-To: <54ec41bc-7cdb-4921-595e-aa302c3f25d7@elitechgroup.com> References: <54ec41bc-7cdb-4921-595e-aa302c3f25d7@elitechgroup.com> Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB358D87AC5CF@mail> Hi Michelle, There is a members only listserv through the American Society for Cytopathology https://www.cytopathology.org/membership-types-requirements/ Or Telephone: (302) 543-6583 Mike -----Original Message----- From: Michelle Jamison via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, October 04, 2018 4:46 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] cytology Hi All, Do you know of a similar list serve that is for Cytologists? Thank you, Michelle -- All the best to you, Michelle Jamison /Bio Research Associate / /ELITechGroup Inc. / ???370 W. 1700 S.?? ???Logan Utah ??? USA m.jamison at elitechgroup.com ? _www.elitechgroup.com _ Tel : +1.435.752.6011 Ext: 1474 Logo _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lindsay.A.Wilson at uth.tmc.edu Fri Oct 5 09:02:33 2018 From: Lindsay.A.Wilson at uth.tmc.edu (Wilson, Lindsay A) Date: Fri, 5 Oct 2018 14:02:33 +0000 Subject: [Histonet] DAB & tissue adherence issues on rabbit bone tissue Message-ID: <2F7418A2-D147-4D69-97B0-DB6CFA1C6DFB@uth.tmc.edu> Good morning, I am currently experiencing an issue with DAB concentrating on certain bone areas of my slide. I am working with decalcified?EDTA , FFPE- rabbit mandible (some tissue attached). It is a new DAB kit from BioCare. It?s thoroughly mixed (and new) before application in a humidity chamber with the slides lying flat. I change out the deparaffinization soluitons frequently, my reagents are in date, I have increased the peroxidase time to 10 minutes, I use a blocking reagent from Millipore kit for 5 minutes (manuf. Recomm.), 2.5% goat serum for 20%, my wash buffer is 1x TBST with 0.05% Tween 20. The FFPE slides are aired dried before use. I have seen posts about baking them in the oven afterward ? that doesn?t affect the slides at all? I also have the issue of the sample falling off and/or moving on my slide. The slides are positively charged, I am currently performing AR with an AR ph6 buffer (for VEGF antibody) in 95C microwave for 15 minutes. I have previously tried a 60C waterbath for 12 hours with the same issue. I am thinking that the issues are connected? If the tissue isn?t moving around or falling off, perhaps the excessive DAB wouldn?t be an issue? I have seen posts about the Biogenex DeCal solution ? is that an effective method? I can attach images if needed. Lindsay Wilson, LVT, RLATG Young Laboratory UTHealth | The University of Texas Health Science Center at Houston | School of Dentistry Department of Oral & Maxillofacial Surgery 7500 Cambridge St. | Suite 6510 | Houston, TX 77054 713 486 4360 tel | 713 486 4333 fax From hinderaker.s at gmail.com Fri Oct 5 12:57:20 2018 From: hinderaker.s at gmail.com (stephanie h) Date: Fri, 5 Oct 2018 11:57:20 -0600 Subject: [Histonet] Special Stain Cost Per Slide Message-ID: <9D532C24-EBF4-437F-9921-26922CD7DB54@gmail.com> Can anyone using a Dako Artisan give me an idea (ballpark is fine) of what your cost per slide is running? Thank you in advance! From SteveM at mcclainlab.com Fri Oct 5 14:33:11 2018 From: SteveM at mcclainlab.com (Steve McClain) Date: Fri, 5 Oct 2018 19:33:11 +0000 Subject: [Histonet] Histonet Digest, Vol 179, Issue 4 AFB control In-Reply-To: References: Message-ID: I was taught it best to use separate controls on a second slide. But in practice, the only cases where a floater appeared it was obvious. In my own lab 14 year experience it has not happened. However, about 10 years ago we started an experiment and switched to 2 AFB slides with pos and neg controls on one slide only but with patients samples on both slides. Looking at 2 slides sometimes makes me more confident. I have not yet competed the study but anecdotally I do not recall or have never seen an AFB floater using this practice. We deliberately keep both our control blocks quite small maybe 1-2 mm. Steve Steve A. McClain, MD 631-361-4000 Cell 631-926-3655 From amosbrooks at gmail.com Fri Oct 5 15:29:54 2018 From: amosbrooks at gmail.com (Amos Brooks) Date: Fri, 5 Oct 2018 16:29:54 -0400 Subject: [Histonet] Histonet Digest, Vol 179, Issue 5 In-Reply-To: References: Message-ID: Hi, > I have a hunch that the DAB deposits you are seeing are not a DAB problem. You didn't mention how long your incubation time is. If I were to venture a guess, I am thinking there may be some evaporation of either the primary or secondary antibody or boil-over from the antigen retrieval. This would give the DAB a place do react. You could use a plastic coverslip or really rinse the slides a lot. I really have not seen any problems with an oven interfering with IHC. I think it is superstition. However, I have seen problems with microwaves. Do yourself a favor and chuck it off the roof of your lab. They do not provide control over temperature. Some areas heat faster than others. They tend to boil over and are always harsh on sensitive tissues like bone. You would be much better off with a hot water bath. It will solve all of these problems. Just my tuppence, Amos Message: 7 Date: Fri, 5 Oct 2018 14:02:33 +0000 From: "Wilson, Lindsay A" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] DAB & tissue adherence issues on rabbit bone tissue Message-ID: <2F7418A2-D147-4D69-97B0-DB6CFA1C6DFB at uth.tmc.edu> Content-Type: text/plain; charset="utf-8" Good morning, I am currently experiencing an issue with DAB concentrating on certain bone areas of my slide. I am working with decalcified?EDTA , FFPE- rabbit mandible (some tissue attached). It is a new DAB kit from BioCare. It?s thoroughly mixed (and new) before application in a humidity chamber with the slides lying flat. I change out the deparaffinization soluitons frequently, my reagents are in date, I have increased the peroxidase time to 10 minutes, I use a blocking reagent from Millipore kit for 5 minutes (manuf. Recomm.), 2.5% goat serum for 20%, my wash buffer is 1x TBST with 0.05% Tween 20. The FFPE slides are aired dried before use. I have seen posts about baking them in the oven afterward ? that doesn?t affect the slides at all? I also have the issue of the sample falling off and/or moving on my slide. The slides are positively charged, I am currently performing AR with an AR ph6 buffer (for VEGF antibody) in 95C microwave for 15 minutes. I have previously tried a 60C waterbath for 12 hours with the same issue. I am thinking that the issues are connected? If the tissue isn?t moving around or falling off, perhaps the excessive DAB wouldn?t be an issue? I have seen posts about the Biogenex DeCal solution ? is that an effective method? I can attach images if needed. Lindsay Wilson, LVT, RLATG Young Laboratory From criley at dpspa.com Mon Oct 8 10:31:08 2018 From: criley at dpspa.com (Charles Riley) Date: Mon, 8 Oct 2018 11:31:08 -0400 Subject: [Histonet] Competency signoff CAP Message-ID: I just recently got put in charge of managing the personnel records for training and am in the process of trying to update our personnel records for our next CAP inspection. I feel our previous files were over detailed and excessive in what they covered and wanted to try and consolidate some of the information under broader categories. Is anyone willing to send me what they use in their labs for Grossing and Histology Competency and proficiency testing sign off for their staff so I can get an idea of how much information I need to provide. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From Timothy.Morken at ucsf.edu Mon Oct 8 10:49:45 2018 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 8 Oct 2018 15:49:45 +0000 Subject: [Histonet] Competency signoff CAP In-Reply-To: References: Message-ID: Charles, for grossing you do need to show competency for the types of specimens they are allowed to gross. Histology "competency" is not required under CLIA because it is not high complexity (no "testing" is done. The "test" is the pathologists interpretation). Therefore, your histology competency is whatever you define within your institution. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, October 08, 2018 8:31 AM To: Histo List Subject: [Histonet] Competency signoff CAP I just recently got put in charge of managing the personnel records for training and am in the process of trying to update our personnel records for our next CAP inspection. I feel our previous files were over detailed and excessive in what they covered and wanted to try and consolidate some of the information under broader categories. Is anyone willing to send me what they use in their labs for Grossing and Histology Competency and proficiency testing sign off for their staff so I can get an idea of how much information I need to provide. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From maxim_71 at mail.ru Mon Oct 8 12:22:40 2018 From: maxim_71 at mail.ru (=?UTF-8?B?0J/QtdGI0LrQvtCyINCc0LDQutGB0LjQvA==?=) Date: Mon, 08 Oct 2018 20:22:40 +0300 Subject: [Histonet] (no subject) Message-ID: <1539019360.34219952@f452.i.mail.ru> Dear Histonetters! I need in your proffessional help. Can you explain for me a the chemical mechanism of Picro-Mallory V stain by chemcial language? I will appreciate references about this issue except original article of Lendrum A.C. et al (1962) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC480427/pdf/jclinpath00070-0009.pdf ? Before asking I was read some histotechnological books, but can not find it. They are: Bancroft&Gamble (from 5 to 8th ed), F. Carson A Self-instructional text 3 ed, C.F.A. Culling (3rd ed), AFIP manuals (3-4 ed), Woods and Ellis (Histology lab: A complete reference), Lilli RD, 1962, Romeis 2014 (18 auflage) and some others book. -- Russia, Taganrog, Maxim Peshkov. From llewllew at shaw.ca Mon Oct 8 13:18:05 2018 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Mon, 8 Oct 2018 11:18:05 -0700 Subject: [Histonet] (no subject) In-Reply-To: <1539019360.34219952@f452.i.mail.ru> References: <1539019360.34219952@f452.i.mail.ru> Message-ID: All of the Picro-Mallory variants are trichrome stains. An explanation of how they work is here: http://stainsfile.info/StainsFile/theory/tri_gen.htm http://stainsfile.info/StainsFile/stain/fibrin/fibrin.htm Follow the links as well for added information. Bryan Llewellyn ?????? ?????? via Histonet wrote: > > Dear Histonetters! > I need in your proffessional help. > Can you explain for me a the chemical mechanism of Picro-Mallory V stain by chemcial language? > I will appreciate references about this issue except original article of Lendrum A.C. et al (1962) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC480427/pdf/jclinpath00070-0009.pdf > Before asking I was read some histotechnological books, but can not find it. They are: Bancroft&Gamble (from 5 to 8th ed), F. Carson A Self-instructional text 3 ed, C.F.A. Culling (3rd ed), AFIP manuals (3-4 ed), Woods and Ellis (Histology lab: A complete reference), Lilli RD, 1962, Romeis 2014 (18 auflage) and some others book. > -- Russia, > Taganrog, > Maxim Peshkov. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Linda.Margraf at cookchildrens.org Tue Oct 9 12:06:27 2018 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Tue, 9 Oct 2018 17:06:27 +0000 Subject: [Histonet] FW: Leica Bond Max artifact In-Reply-To: <80A8A08D-0AD4-492D-AA2F-BD521EBDC6D0@gmail.com> References: <80A8A08D-0AD4-492D-AA2F-BD521EBDC6D0@gmail.com> Message-ID: Here is a message I am posting for Dr. Rosen: Hello, I am a longtime admirer of this list, and I am sure someone on here can help us out We have an artifact on our immunohistochemistry (IHC) slides. I will attempt to attach pictures using the upload tool. In case that doesn?t work, I am attaching a google drive link to some jpgs. The artifact is usually golden brown to red/pink in color, is clustered, appears both on top of the tissue and around it. If we do an aggressive rundown, it appears less red/brown and bluer. We are using a Leica Bond MAX IHC system which is about a year old. We usually do two runs a day and 95% of the time we only use a red detection kit. We only see this on our red detection kit on our Leica bond. The brown is unaffected. The corresponding H&E slides are fine. We started having this artifact after a month or two of production. 85% of the time we have some sort of artifact. Sometimes its perfect, sometimes it makes the tissue unreadable. We haven?t figured out any precipitating factors. We have tried a number of things, but we still can?t get control of this. Here is a brief overview of our protocols: - Processor: Leica ASP300s tissue processor. -Parrafin = Mercedes HWWX (dual purpose embedding and infiltration parrafin) melting point 57c ? we tried switching, and it didn?t help. - We only use distilled water in our baths - Mercedes Starfrost charged slides - Oven for 30 min at 62c - We always use a cold red detection kit -Sometimes our runs are delayed but not more than 4 hours - Rundown is done in our stainer (standardized), and slides are coverslipped with tape. - The bulk fluid containers in the bond are cleaned weekly and refrigerated between runs. - The probe is cleaned every 300 slides. - Mixing wells are cleaned out every two weeks and replaced often. -Covertiles are well tended to. We have tried to correct this problem, but we can't seem to fix it. Any ideas? https://drive.google.com/open?id=1wUttTweP7SYLRM5pkGOBr0MBzCMrtJSA Jason R. Rosen, D.O. Dermatopathologist Premier Dermatology Partners 4675 Linton Boulevard, Suite 203 Delray Beach, FL 33445 (p) 561-499-5341 (f) 561-499-5343 (e) JRosen at totalderms.com From rjbuesa at yahoo.com Tue Oct 9 12:46:13 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 9 Oct 2018 17:46:13 +0000 (UTC) Subject: [Histonet] FW: Leica Bond Max artifact In-Reply-To: References: <80A8A08D-0AD4-492D-AA2F-BD521EBDC6D0@gmail.com> Message-ID: <13494929.7046711.1539107173240@mail.yahoo.com> Dear Dr. Rosen: I see nothing wrong in your protocol but your photo 0950 = 09501 seems as if there are 2 superimposed sections, one with a lot of brown particles? Is this artifact you are referring to? Seems "too organized" to be an artifact but it is brown and not red. These "non DAB" reagents sometimes develop a sort of precipitate even within their expiration date. Why have you decided to use this red reagent instead of DAB? That could be a better option. Other than that I am also completely baffled by your problem. Ren? On Tuesday, October 9, 2018 1:15 PM, Linda Margraf via Histonet wrote: Here is a message I am posting for Dr. Rosen: Hello, I am a longtime admirer of this list, and I am sure someone on here can help us out We have an artifact on our immunohistochemistry (IHC) slides. I will attempt to attach pictures using the upload tool. In case that doesn?t work, I am attaching a google drive link to some jpgs. The artifact is usually golden brown to red/pink in color, is clustered, appears both on top of the tissue and around it. If we do an aggressive rundown, it appears less red/brown and bluer. We are using a Leica Bond MAX IHC system which is about a year old. We usually do two runs a day and 95% of the time we only use a red detection kit. We only see this on our red detection kit on our Leica bond. The brown is unaffected. The corresponding H&E slides are fine. We started having this artifact after a month or two of production. 85% of the time we have some sort of artifact. Sometimes its perfect, sometimes it makes the tissue unreadable. We haven?t figured out any precipitating factors. We have tried a number of things, but we still can?t get control of this. Here is a brief overview of our protocols: - Processor: Leica ASP300s tissue processor. -Parrafin = Mercedes HWWX (dual purpose embedding and infiltration parrafin) melting point 57c ? we tried switching, and it didn?t help. - We only use distilled water in our baths - Mercedes Starfrost charged slides - Oven for 30 min at 62c - We always use a cold red detection kit -Sometimes our runs are delayed but not more than 4 hours - Rundown is done in our stainer (standardized), and slides are coverslipped with tape. - The bulk fluid containers in the bond are cleaned weekly and refrigerated between runs. - The probe is cleaned every 300 slides. - Mixing wells are cleaned out every two weeks and replaced often. -Covertiles are well tended to. We have tried to correct this problem, but we can't seem to fix it. Any ideas? https://drive.google.com/open?id=1wUttTweP7SYLRM5pkGOBr0MBzCMrtJSA Jason R. Rosen, D.O. Dermatopathologist Premier Dermatology Partners 4675 Linton Boulevard, Suite 203 Delray Beach, FL 33445 (p) 561-499-5341 (f) 561-499-5343 (e) JRosen at totalderms.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Tue Oct 9 12:49:58 2018 From: patpxs at gmail.com (P Sicurello) Date: Tue, 9 Oct 2018 10:49:58 -0700 Subject: [Histonet] H&E for H. pylori? Message-ID: Good Morning Listers, Has anyone out there optimized an H&E for H. pylori? Sure we can run a giemsa or and IHC but what fun is that? Send me your ideas and make the day of our GI pathologists. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From rjbuesa at yahoo.com Tue Oct 9 14:46:01 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 9 Oct 2018 19:46:01 +0000 (UTC) Subject: [Histonet] H&E for H. pylori? In-Reply-To: References: Message-ID: <1870025542.7160799.1539114361850@mail.yahoo.com> Paula:"Optimizing" H&E to detect H. piloris is a rout you should not attempt?because H&E cannot be "optimized" for that.Giemsa is?one way or, even better, modified Steiner stain. The only disadvantage modified Steiner has?is using radioactive uranium nitrate, but I developed a procedure where 0.02%?aq. phosphotungstic acid solution can be used instead.?IHC will be way more expensive. Ren? On Tuesday, October 9, 2018 2:03 PM, P Sicurello via Histonet wrote: Good Morning Listers, Has anyone out there optimized an H&E for H. pylori? Sure we can run a giemsa or and IHC but what fun is that? Send me your ideas and make the day of our GI pathologists. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Tue Oct 9 17:59:15 2018 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Tue, 9 Oct 2018 22:59:15 +0000 Subject: [Histonet] H&E for H. pylori? In-Reply-To: <1870025542.7160799.1539114361850@mail.yahoo.com> References: <1870025542.7160799.1539114361850@mail.yahoo.com> Message-ID: The following might be useful: Tazawa, K., & Tsutsumi, Y. (1998). Effect of prolonged staining with hematoxylin on detecting Helicobacter pyiori in hematoxylin?eosin?stained gastric mucosa. Pathology international, 48(6), 448-452. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 10 October 2018 6:46 AM To: P Sicurello; HistoNet Subject: Re: [Histonet] H&E for H. pylori? Paula:"Optimizing" H&E to detect H. piloris is a rout you should not attempt?because H&E cannot be "optimized" for that.Giemsa is?one way or, even better, modified Steiner stain. The only disadvantage modified Steiner has?is using radioactive uranium nitrate, but I developed a procedure where 0.02%?aq. phosphotungstic acid solution can be used instead.?IHC will be way more expensive. Ren? On Tuesday, October 9, 2018 2:03 PM, P Sicurello via Histonet wrote: Good Morning Listers, Has anyone out there optimized an H&E for H. pylori? Sure we can run a giemsa or and IHC but what fun is that? Send me your ideas and make the day of our GI pathologists. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From esarricks at gmail.com Tue Oct 9 18:33:06 2018 From: esarricks at gmail.com (Erin Sarricks) Date: Tue, 9 Oct 2018 19:33:06 -0400 Subject: [Histonet] Job Opening in Massachusetts: IHC Lab Supervisor Message-ID: HSRL (Histo-Scientific Research Labs) is a leading GLP contract research organization which specializes in veterinary histopathology since 1999. Our staff has been committed to scientific integrity and superior responsiveness to client needs. A career with HSRL offers you potential to join an established company with exceptional growth opportunity. HSRL is seeking a qualified Immunohistochemistry Laboratory Supervisor to join our team at our facility in Worcester, MA. This position is split between bench histology/IHC and managing a small team of specialists. Essential Functions and Responsibilities: - Perform routine histology, including tissue processing, fixation, embedding, sectioning, staining, and imaging with the highest of quality. - Oversee GLP IHC program. - Develop Immunohistochemistry stain protocols and adhere to write SOPs. - Effectively collaborate with team members and clients - Various administrative duties to include recording and maintaining detailed records - Excellent organizational skills and ability to multi-task - Works independently and troubleshoots technical problems Qualifications: - Minimum 3 years of related experience as a histologic technician with concentration in veterinary histopathology and IHC techniques. - ASCP certified Histotechnician (HT) or Histotechnologist (HTL) or eligible, certification preferred. - Demonstrated knowledge of and skill in oral and written communication, problem solving, adaptability, and teamwork. - Excellent organizational skills and ability to multi-task. - Experience with Biocare?s IntelliPATH Autostainer is preferred. A comprehensive benefits package will be offered to the successful candidate, salary commensurate with experience. To learn more about HSRL, please visit http://www.hsrl.org Job Type: Full-time Experience: - employment as a Histo Tech w/concentration in IHC techniques: 3 years (Required) From bcooper at chla.usc.edu Tue Oct 9 18:34:13 2018 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Tue, 9 Oct 2018 23:34:13 +0000 Subject: [Histonet] Low Oxygen Sensor/Alarms Message-ID: Hi, Looking at the newly revised LABGEN checklist this afternoon. GEN.77550 states "In areas where liquid nitrogen is used, there are oxygen sensors with a low oxygen alarm mounted in an appropriate location and sufficient airflow to prevent asphyxiation." For those of you who already do this, can you please tell me the type and model of sensor you're using? Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From cmmathis at wakehealth.edu Wed Oct 10 12:41:02 2018 From: cmmathis at wakehealth.edu (Cathy M. Mathis/Comparative Medicine) Date: Wed, 10 Oct 2018 17:41:02 +0000 Subject: [Histonet] Leica Bond Max artifact Message-ID: <4816F916B11AEC4E92A0D01372F573E2012CFD24D9@EXCHDB8.medctr.ad.wfubmc.edu> Hello Dr. Rosen and Histonetters, We have used a Leica Bond RX for over 2 years now, cleaning and caring for it just as you do. My work is mostly animal which is why we mainly choose the red chromogen alkaline phosphatase detection kit. We see the same artifact sporadically. Sometimes we will see it for several days and then maybe a week goes by and we don't see any of it. It may only be a small amount and I have seen it basically all over the slide. I know it is not related to our tissues or slides. Our Leica rep came out and increased washes but it really doesn't help. If anyone has a solution to the problem, I would also be interested. Best to all, Cathy Cathy M. Mathis Laboratory Technician IV Department of Pathology Section on Comparative Medicine - Clarkson Campus Medical Center Boulevard ?\? Winston-Salem, NC 27157 p 336.716.1538?\? f 336.716.1515? cmmathis at wakehealth.edu wakehealth.edu -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Wednesday, October 10, 2018 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 179, Issue 9 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w&r=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg&m=UD2XLalsIAuK_lUZgavLUGnMMJaE8-g4Q28xJLvJLAw&s=65VTIbTt5Cez3DHVF4vIBCoqH0MsYay3VSIywTmpZfw&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. FW: Leica Bond Max artifact (Linda Margraf) 2. Re: FW: Leica Bond Max artifact (Rene J Buesa) 3. H&E for H. pylori? (P Sicurello) 4. Re: H&E for H. pylori? (Rene J Buesa) 5. Re: H&E for H. pylori? (Tony Henwood (SCHN)) 6. Job Opening in Massachusetts: IHC Lab Supervisor (Erin Sarricks) 7. Low Oxygen Sensor/Alarms (Cooper, Brian) ---------------------------------------------------------------------- Message: 1 Date: Tue, 9 Oct 2018 17:06:27 +0000 From: Linda Margraf To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] FW: Leica Bond Max artifact Message-ID: Content-Type: text/plain; charset="utf-8" Here is a message I am posting for Dr. Rosen: Hello, I am a longtime admirer of this list, and I am sure someone on here can help us out We have an artifact on our immunohistochemistry (IHC) slides. I will attempt to attach pictures using the upload tool. In case that doesn?t work, I am attaching a google drive link to some jpgs. The artifact is usually golden brown to red/pink in color, is clustered, appears both on top of the tissue and around it. If we do an aggressive rundown, it appears less red/brown and bluer. We are using a Leica Bond MAX IHC system which is about a year old. We usually do two runs a day and 95% of the time we only use a red detection kit. We only see this on our red detection kit on our Leica bond. The brown is unaffected. The corresponding H&E slides are fine. We started having this artifact after a month or two of production. 85% of the time we have some sort of artifact. Sometimes its perfect, sometimes it makes the tissue unreadable. We haven?t figured out any precipitating factors. We have tried a number of things, but we still can?t get control of this. Here is a brief overview of our protocols: - Processor: Leica ASP300s tissue processor. -Parrafin = Mercedes HWWX (dual purpose embedding and infiltration parrafin) melting point 57c ? we tried switching, and it didn?t help. - We only use distilled water in our baths - Mercedes Starfrost charged slides - Oven for 30 min at 62c - We always use a cold red detection kit -Sometimes our runs are delayed but not more than 4 hours - Rundown is done in our stainer (standardized), and slides are coverslipped with tape. - The bulk fluid containers in the bond are cleaned weekly and refrigerated between runs. - The probe is cleaned every 300 slides. - Mixing wells are cleaned out every two weeks and replaced often. -Covertiles are well tended to. We have tried to correct this problem, but we can't seem to fix it. Any ideas? https://urldefense.proofpoint.com/v2/url?u=https-3A__drive.google.com_open-3Fid-3D1wUttTweP7SYLRM5pkGOBr0MBzCMrtJSA&d=DwICAg&c=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w&r=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg&m=UD2XLalsIAuK_lUZgavLUGnMMJaE8-g4Q28xJLvJLAw&s=kyqBaPGz-SvAoNDooiBtkSemt9dk4eOKo8l1XYvtmIs&e= Jason R. Rosen, D.O. Dermatopathologist Premier Dermatology Partners 4675 Linton Boulevard, Suite 203 Delray Beach, FL 33445 (p) 561-499-5341 (f) 561-499-5343 (e) JRosen at totalderms.com ------------------------------ Message: 2 Date: Tue, 9 Oct 2018 17:46:13 +0000 (UTC) From: Rene J Buesa To: Linda Margraf , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] FW: Leica Bond Max artifact Message-ID: <13494929.7046711.1539107173240 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Dear Dr. Rosen: I see nothing wrong in your protocol but your photo 0950 = 09501 seems as if there are 2 superimposed sections, one with a lot of brown particles? Is this artifact you are referring to? Seems "too organized" to be an artifact but it is brown and not red. These "non DAB" reagents sometimes develop a sort of precipitate even within their expiration date. Why have you decided to use this red reagent instead of DAB? That could be a better option. Other than that I am also completely baffled by your problem. Ren? On Tuesday, October 9, 2018 1:15 PM, Linda Margraf via Histonet wrote: Here is a message I am posting for Dr. Rosen: Hello, I am a longtime admirer of this list, and I am sure someone on here can help us out We have an artifact on our immunohistochemistry (IHC) slides. I will attempt to attach pictures using the upload tool. In case that doesn?t work, I am attaching a google drive link to some jpgs. The artifact is usually golden brown to red/pink in color, is clustered, appears both on top of the tissue and around it. If we do an aggressive rundown, it appears less red/brown and bluer. We are using a Leica Bond MAX IHC system which is about a year old. We usually do two runs a day and 95% of the time we only use a red detection kit. We only see this on our red detection kit on our Leica bond. The brown is unaffected. The corresponding H&E slides are fine. We started having this artifact after a month or two of production. 85% of the time we have some sort of artifact. Sometimes its perfect, sometimes it makes the tissue unreadable. We haven?t figured out any precipitating factors. We have tried a number of things, but we still can?t get control of this. Here is a brief overview of our protocols: - Processor: Leica ASP300s tissue processor. -Parrafin = Mercedes HWWX (dual purpose embedding and infiltration parrafin) melting point 57c ? we tried switching, and it didn?t help. - We only use distilled water in our baths - Mercedes Starfrost charged slides - Oven for 30 min at 62c - We always use a cold red detection kit -Sometimes our runs are delayed but not more than 4 hours - Rundown is done in our stainer (standardized), and slides are coverslipped with tape. - The bulk fluid containers in the bond are cleaned weekly and refrigerated between runs. - The probe is cleaned every 300 slides. - Mixing wells are cleaned out every two weeks and replaced often. -Covertiles are well tended to. We have tried to correct this problem, but we can't seem to fix it. Any ideas? https://urldefense.proofpoint.com/v2/url?u=https-3A__drive.google.com_open-3Fid-3D1wUttTweP7SYLRM5pkGOBr0MBzCMrtJSA&d=DwICAg&c=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w&r=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg&m=UD2XLalsIAuK_lUZgavLUGnMMJaE8-g4Q28xJLvJLAw&s=kyqBaPGz-SvAoNDooiBtkSemt9dk4eOKo8l1XYvtmIs&e= Jason R. Rosen, D.O. Dermatopathologist Premier Dermatology Partners 4675 Linton Boulevard, Suite 203 Delray Beach, FL 33445 (p) 561-499-5341 (f) 561-499-5343 (e) JRosen at totalderms.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w&r=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg&m=UD2XLalsIAuK_lUZgavLUGnMMJaE8-g4Q28xJLvJLAw&s=65VTIbTt5Cez3DHVF4vIBCoqH0MsYay3VSIywTmpZfw&e= ------------------------------ Message: 3 Date: Tue, 9 Oct 2018 10:49:58 -0700 From: P Sicurello To: HistoNet Subject: [Histonet] H&E for H. pylori? Message-ID: Content-Type: text/plain; charset="UTF-8" Good Morning Listers, Has anyone out there optimized an H&E for H. pylori? Sure we can run a giemsa or and IHC but what fun is that? Send me your ideas and make the day of our GI pathologists. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ------------------------------ Message: 4 Date: Tue, 9 Oct 2018 19:46:01 +0000 (UTC) From: Rene J Buesa To: P Sicurello , HistoNet Subject: Re: [Histonet] H&E for H. pylori? Message-ID: <1870025542.7160799.1539114361850 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Paula:"Optimizing" H&E to detect H. piloris is a rout you should not attempt?because H&E cannot be "optimized" for that.Giemsa is?one way or, even better, modified Steiner stain. The only disadvantage modified Steiner has?is using radioactive uranium nitrate, but I developed a procedure where 0.02%?aq. phosphotungstic acid solution can be used instead.?IHC will be way more expensive. Ren? On Tuesday, October 9, 2018 2:03 PM, P Sicurello via Histonet wrote: Good Morning Listers, Has anyone out there optimized an H&E for H. pylori? Sure we can run a giemsa or and IHC but what fun is that? Send me your ideas and make the day of our GI pathologists. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w&r=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg&m=UD2XLalsIAuK_lUZgavLUGnMMJaE8-g4Q28xJLvJLAw&s=65VTIbTt5Cez3DHVF4vIBCoqH0MsYay3VSIywTmpZfw&e= ------------------------------ Message: 5 Date: Tue, 9 Oct 2018 22:59:15 +0000 From: "Tony Henwood (SCHN)" To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] H&E for H. pylori? Message-ID: Content-Type: text/plain; charset="utf-8" The following might be useful: Tazawa, K., & Tsutsumi, Y. (1998). Effect of prolonged staining with hematoxylin on detecting Helicobacter pyiori in hematoxylin?eosin?stained gastric mucosa. Pathology international, 48(6), 448-452. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 10 October 2018 6:46 AM To: P Sicurello; HistoNet Subject: Re: [Histonet] H&E for H. pylori? Paula:"Optimizing" H&E to detect H. piloris is a rout you should not attempt?because H&E cannot be "optimized" for that.Giemsa is?one way or, even better, modified Steiner stain. The only disadvantage modified Steiner has?is using radioactive uranium nitrate, but I developed a procedure where 0.02%?aq. phosphotungstic acid solution can be used instead.?IHC will be way more expensive. Ren? On Tuesday, October 9, 2018 2:03 PM, P Sicurello via Histonet wrote: Good Morning Listers, Has anyone out there optimized an H&E for H. pylori? Sure we can run a giemsa or and IHC but what fun is that? Send me your ideas and make the day of our GI pathologists. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material.? Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited.? If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w&r=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg&m=UD2XLalsIAuK_lUZgavLUGnMMJaE8-g4Q28xJLvJLAw&s=65VTIbTt5Cez3DHVF4vIBCoqH0MsYay3VSIywTmpZfw&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w&r=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg&m=UD2XLalsIAuK_lUZgavLUGnMMJaE8-g4Q28xJLvJLAw&s=65VTIbTt5Cez3DHVF4vIBCoqH0MsYay3VSIywTmpZfw&e= This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ------------------------------ Message: 6 Date: Tue, 9 Oct 2018 19:33:06 -0400 From: Erin Sarricks To: histonet Subject: [Histonet] Job Opening in Massachusetts: IHC Lab Supervisor Message-ID: Content-Type: text/plain; charset="UTF-8" HSRL (Histo-Scientific Research Labs) is a leading GLP contract research organization which specializes in veterinary histopathology since 1999. Our staff has been committed to scientific integrity and superior responsiveness to client needs. A career with HSRL offers you potential to join an established company with exceptional growth opportunity. HSRL is seeking a qualified Immunohistochemistry Laboratory Supervisor to join our team at our facility in Worcester, MA. This position is split between bench histology/IHC and managing a small team of specialists. Essential Functions and Responsibilities: - Perform routine histology, including tissue processing, fixation, embedding, sectioning, staining, and imaging with the highest of quality. - Oversee GLP IHC program. - Develop Immunohistochemistry stain protocols and adhere to write SOPs. - Effectively collaborate with team members and clients - Various administrative duties to include recording and maintaining detailed records - Excellent organizational skills and ability to multi-task - Works independently and troubleshoots technical problems Qualifications: - Minimum 3 years of related experience as a histologic technician with concentration in veterinary histopathology and IHC techniques. - ASCP certified Histotechnician (HT) or Histotechnologist (HTL) or eligible, certification preferred. - Demonstrated knowledge of and skill in oral and written communication, problem solving, adaptability, and teamwork. - Excellent organizational skills and ability to multi-task. - Experience with Biocare?s IntelliPATH Autostainer is preferred. A comprehensive benefits package will be offered to the successful candidate, salary commensurate with experience. To learn more about HSRL, please visit https://urldefense.proofpoint.com/v2/url?u=http-3A__www.hsrl.org&d=DwICAg&c=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w&r=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg&m=UD2XLalsIAuK_lUZgavLUGnMMJaE8-g4Q28xJLvJLAw&s=A01xuw7HuwFrJIsZpyy99R_tWx6s6vclF1gInPKKpsw&e= Job Type: Full-time Experience: - employment as a Histo Tech w/concentration in IHC techniques: 3 years (Required) ------------------------------ Message: 7 Date: Tue, 9 Oct 2018 23:34:13 +0000 From: "Cooper, Brian" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Low Oxygen Sensor/Alarms Message-ID: Content-Type: text/plain; charset=us-ascii Hi, Looking at the newly revised LABGEN checklist this afternoon. GEN.77550 states "In areas where liquid nitrogen is used, there are oxygen sensors with a low oxygen alarm mounted in an appropriate location and sufficient airflow to prevent asphyxiation." For those of you who already do this, can you please tell me the type and model of sensor you're using? Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w&r=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg&m=UD2XLalsIAuK_lUZgavLUGnMMJaE8-g4Q28xJLvJLAw&s=65VTIbTt5Cez3DHVF4vIBCoqH0MsYay3VSIywTmpZfw&e= ------------------------------ End of Histonet Digest, Vol 179, Issue 9 **************************************** From mtighe at trudeauinstitute.org Wed Oct 10 14:05:34 2018 From: mtighe at trudeauinstitute.org (Mike Tighe) Date: Wed, 10 Oct 2018 19:05:34 +0000 Subject: [Histonet] Large bone samples Message-ID: Hi Everyone, I am looking into doing some histology (HandE) on NHP joints. Samples will be finger joints and wrist. If you have any experience with these larger bones (and soft tissue) I would be interested in any advice on general time and type of decal solution. I only need to do H&E no immuno so I think acid decal is OK. Thanks for any help!! Mike From CDavis at che-east.org Wed Oct 10 14:35:21 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Wed, 10 Oct 2018 19:35:21 +0000 Subject: [Histonet] H&E for H. pylori Message-ID: <322cf07c507d41d1a690aa1a02644714@che-east.org> In the beginning of my last FT job we had a excellent female pathologist who could see the H. pylori on SurgiPath's H&E stain. I learned so much from her and dearly missed her toward the end. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From greg.dobbin at gmail.com Thu Oct 11 10:04:15 2018 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Thu, 11 Oct 2018 12:04:15 -0300 Subject: [Histonet] Leica Bond Max artifact Message-ID: Hi Linda, Is this a problem that has just started or has the red kit always had this artefact? - If it just started, perhaps it is a problem with a particular kit lot number (or numbers). If so, get Leica Tech service involved to see if anyone else has reported a problem. - If it has been an ongoing issue for the red kit then perhaps you need to modify the Red Kit protocol by adding additional (and/or longer) wash steps. Here again Tech Service can help you with this. - The only other thing I can think to ask is: Do you have anyone new operating the instrument? Could a new person be putting undiluted Bond Wash in the bulk container? Let us know what you find out. Good luck. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From Cnituda at nvdermatology.com Thu Oct 11 12:29:26 2018 From: Cnituda at nvdermatology.com (Carl Nituda) Date: Thu, 11 Oct 2018 17:29:26 +0000 Subject: [Histonet] Pathology Consult in Hospital and technical bill in private lab Message-ID: <31097a71f67a4ebf8619011584a9d370@NVDX.nvdc.domain> Hi Everyone, We are a dermatology center here in norcal and ocassionally our physicians are on call for stat cases at local hospital. Can our physician bring the biopsy from that stat case so we can do the technical component too so we can then bill both the professional and technical? Regards, Carl From jrosen at totalderms.com Thu Oct 11 12:36:12 2018 From: jrosen at totalderms.com (Dr. Jason Rosen) Date: Thu, 11 Oct 2018 17:36:12 +0000 Subject: [Histonet] Leica Bond Max artifact In-Reply-To: References: Message-ID: Hi Greg, It has been a problem for months across hundreds of kits. We added some washes and more dips in the DI water after the run to the protocol. It helps a little bit. I am going to try a more things in the next few weeks and I will keep the community updated. -----Original Message----- From: Greg Dobbin Sent: Thursday, October 11, 2018 11:04 AM To: Linda.Margraf at cookchildrens.org Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Leica Bond Max artifact Hi Linda, Is this a problem that has just started or has the red kit always had this artefact? - If it just started, perhaps it is a problem with a particular kit lot number (or numbers). If so, get Leica Tech service involved to see if anyone else has reported a problem. - If it has been an ongoing issue for the red kit then perhaps you need to modify the Red Kit protocol by adding additional (and/or longer) wash steps. Here again Tech Service can help you with this. - The only other thing I can think to ask is: Do you have anyone new operating the instrument? Could a new person be putting undiluted Bond Wash in the bulk container? Let us know what you find out. Good luck. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From gu.lang at gmx.at Thu Oct 11 11:58:12 2018 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 11 Oct 2018 18:58:12 +0200 Subject: [Histonet] VIP issue Message-ID: <000301d46183$9911d600$cb358200$@gmx.at> Dear all! I have a question for those, who are familiar with the VIPs from Sakura. Last time we changed the reagenses the cleaning-ethanol (96%) was very milky and even was full of many small particles. It was a paraffin-soup. What is the cause for such a case? We have been using the organic solvent (ShellSol) for decades as xylensubstitute. Maybe the quality has suffered and the ability of solving paraffin has decreased. But are there other explanations? Maybe a malfunction of the instrument? Thanks in advance Gudrun Lang From relia1 at earthlink.net Thu Oct 11 13:28:11 2018 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 11 Oct 2018 14:28:11 -0400 Subject: [Histonet] We are Brainstorming on Promoting the Field of Histology and We Need Your Help! Message-ID: <026c01d46190$65922b10$30b68130$@earthlink.net> Hi Histonetters, How are you doing today? This e-mail is a little different than the normal e-mail that I send out because I am working on a project and I need your help I do have some great jobs but first I am doing some research on how to get the word out on histology as a profession. I am very passionate about this! For one thing had I known about the field of histology when I was starting out, I believe I would be sitting in front of a microtome today instead of a computer!!! Histonetters, the help I need is this I am going to ask you to brainstorm with me about the ?who?s, what?s, why?s, when?s, and how?s of what lead you to the field of histology. I hope you will share your thoughts with me. As always your contributions will be confidential and I will be happy to share with you the ultimate results of the project I am working on. Here are my questions: 1. How did you find out about the field of histology? 2. What made you decide to become a histologist? 3. If you were to do a career day at a high school what would you do to make the presentation exciting and interesting? I also have some exciting job opportunities to pass along. Some of these are new and some of them have updated information so please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral rewards! I LOVE TO GIVE REFERRAL REWARDS!! SOME of these are RELIA exclusives MOST of these offer Sign- On Bonuses and/or Relocation Assistance ALL of these Companies offer excellent compensation, benefits and great environments. AND THEY ALL ARE READY TO HIRE!! If You Or Anyone You Know Might Be Interested In Any Of These Positions Or would like a customized job search in another area. Please Contact Me! You can reach me by email at relia1 at earthlink.net Toll free at 866-607-3542 or on my cell at 407-353-5070. Here are the exciting opportunities I am talking about: Spotlight Opportunity A RELIA EXCLUSIVE: Territory Account Manager ? Histology Sales! ? TX, NM, OK, AR MS and LA!!! Histology Leadership Opportunities Available in: North Carolina Histology Lab Manager Georgia Histology Lab Manager Virginia Senior Histotechnologist TX, NM, OK, AR, MS & LA Histology Sales A RELIA EXCLUSIVE! Histotechnician/Histotechnologist Opportunities Available in: TX, NM, OK, AR, MS & LA Histology Sales A RELIA EXCLUSIVE! Fayetteville, NC Great Place, relo assistance! Norfolk, VA DAYS!! Sign on bonus and relo! Kansas City, MO State of the art lab, great team San Diego, CA Days ? CLIA qualified to gross Hammond, IN Great bennies, sign on bonus and relo! Fayetteville, AR Days CLIA qualified to gross Tallahassee, FL Great team, Night Shift Have a great day. I look forward to hearing back from you! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From saby_joseph_a at yahoo.com Thu Oct 11 13:59:01 2018 From: saby_joseph_a at yahoo.com (Joseph Saby) Date: Thu, 11 Oct 2018 18:59:01 +0000 (UTC) Subject: [Histonet] VIP issue In-Reply-To: <000301d46183$9911d600$cb358200$@gmx.at> References: <000301d46183$9911d600$cb358200$@gmx.at> Message-ID: <863943052.8885657.1539284341399@mail.yahoo.com> Gudrun- Assuming this is a new issue with a recent reagent change, I suspect that the alcohol used for the clean cycle was not 100% (maybe 95%?). The ability of your alcohol to hold the paraffin in solution is lost as the percentage of water increases. I also expect your solvent was mostly saturated with paraffin. Try changing your clean cycle reagents and see if the situation improves. I would do this before processing tissue again on this unit. I hope this helps. Joe Saby On Thursday, October 11, 2018, 1:48:54 PM EDT, Gudrun Lang via Histonet wrote: Dear all! I have a question for those, who are familiar with the VIPs from Sakura. Last time we changed the reagenses the cleaning-ethanol (96%) was very milky and even was full of many small particles. It was a paraffin-soup. What is the cause for such a case?? We have been using the organic solvent (ShellSol) for decades as xylensubstitute. Maybe the quality has suffered and the ability of solving paraffin has decreased. But are there other explanations? Maybe a malfunction of the instrument? Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From boznpl at aol.com Thu Oct 11 14:00:34 2018 From: boznpl at aol.com (Laurie Redmond) Date: Thu, 11 Oct 2018 15:00:34 -0400 Subject: [Histonet] VIP issue In-Reply-To: <000301d46183$9911d600$cb358200$@gmx.at> References: <000301d46183$9911d600$cb358200$@gmx.at> Message-ID: <16664807c30-1ec3-c9a@webjasstg-vab14.srv.aolmail.net> Either: 1) the ShellSol was over-used and could no longer clean/dissolve the paraffin so the remaining paraffin ended up in the alcohol? 2) There was water in the cleaning alcohol (we always use 100% alcohol - do you always use 96%?? 3) the cleaning ShellSol and cleaning alcohols were switched 4) there was a machine malfunction Good Luck! Laurie Redmond -----Original Message----- From: Gudrun Lang via Histonet To: histonet Sent: Thu, Oct 11, 2018 10:58 am Subject: [Histonet] VIP issue Dear all! I have a question for those, who are familiar with the VIPs from Sakura. Last time we changed the reagenses the cleaning-ethanol (96%) was very milky and even was full of many small particles. It was a paraffin-soup. What is the cause for such a case? We have been using the organic solvent (ShellSol) for decades as xylensubstitute. Maybe the quality has suffered and the ability of solving paraffin has decreased. But are there other explanations? Maybe a malfunction of the instrument? Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From VKurth at uwhealth.org Fri Oct 12 06:58:33 2018 From: VKurth at uwhealth.org (Kurth Virginia L.) Date: Fri, 12 Oct 2018 11:58:33 +0000 Subject: [Histonet] Her2 dual ISH Message-ID: Hello I was reading the past posts of inconsistent her2 dual ISH results on the VMS Ultra. I am wondering if anyone has unlocked the secret of getting consistent results? Thank you advance! Ginny From gu.lang at gmx.at Fri Oct 12 12:02:14 2018 From: gu.lang at gmx.at (Gudrun Lang) Date: Fri, 12 Oct 2018 19:02:14 +0200 Subject: [Histonet] VIP issue In-Reply-To: <000301d46183$9911d600$cb358200$@gmx.at> References: <000301d46183$9911d600$cb358200$@gmx.at> Message-ID: <000c01d4624d$53953c00$fabfb400$@gmx.at> Thank you for all the good hints and advices. It has to be a reagens-issue, because the second instrument was also concerned. But we had no similar problems for decades, although we use 96% as cleaning-ethanol after ShellSol. We change the reagenses after 5 runs, once a week. We soon are forced to end using ShellSol, because the company stops the production. We don't like to switch to xylene. Which xylen-substitutes are recommended for the VIP besides of Tissue-Clear (Sakura)? Best wishes Gudrun -----Urspr?ngliche Nachricht----- Von: Gudrun Lang via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Donnerstag, 11. Oktober 2018 18:58 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] VIP issue Dear all! I have a question for those, who are familiar with the VIPs from Sakura. Last time we changed the reagenses the cleaning-ethanol (96%) was very milky and even was full of many small particles. It was a paraffin-soup. What is the cause for such a case? We have been using the organic solvent (ShellSol) for decades as xylensubstitute. Maybe the quality has suffered and the ability of solving paraffin has decreased. But are there other explanations? Maybe a malfunction of the instrument? Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa at yahoo.com Fri Oct 12 12:34:21 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 12 Oct 2018 17:34:21 +0000 (UTC) Subject: [Histonet] VIP issue In-Reply-To: <000c01d4624d$53953c00$fabfb400$@gmx.at> References: <000301d46183$9911d600$cb358200$@gmx.at> <000c01d4624d$53953c00$fabfb400$@gmx.at> Message-ID: <408160669.8990893.1539365662012@mail.yahoo.com> Gudrun: I have used/recommended for 10 years now graded?isopropyl (2-propanol) alcohol to dehydrate and before paraffin ONLY and when I?went to Australia in 2011 found out that they?had been?using the same protocol for years. You just grade 2-propanol in the same way you grade ethanol (60%?80%?90%?100%) and continue DIRECTLY to paraffin. Usually I dedicate one "hot" (paraffin) VIP station?with a 1:1 of 2-propanol + paraffin mixture to facilitate the infiltration or (better yet) one "hot" station with a 1:1 mixture of 2-propanol + pure mineral oil (= paraffin of low molecular weight) and the infiltration is really excellent. In this way you will be able to eliminate BOTH xylene and any other "friendly" clearing agent (ALL?are toxic lat different evels). 2-propanol is NOT toxic AT ALL! I have ALSO stopped using xylene to de-wax sections before staining and substituted it?with a 2% aq. sol. of dishwashing soap and go to water ? staining after (I can send you the detailed protocol / publications). AFTER staining I just rinse the stained slides in 2-propalo ? shake it well ? to dry oven at 60?C for 10 minutes ? coverslip. My lab was TOTALLY xylene free, and you can do the same thing. If you are hesitant just TRY these methods with only few blocks / sections / stained sections and convince yourself. Best regards Ren? On Friday, October 12, 2018 1:13 PM, Gudrun Lang via Histonet wrote: Thank you for all the good hints and advices. It has to be a reagens-issue, because the second instrument was also concerned. But we had no similar problems for decades, although we use 96% as cleaning-ethanol after ShellSol. We change the reagenses after 5 runs, once a week. We soon are forced to end using ShellSol, because the company stops the production. We don't like to switch to xylene. Which xylen-substitutes are recommended for the VIP besides of Tissue-Clear (Sakura)? Best wishes Gudrun -----Urspr?ngliche Nachricht----- Von: Gudrun Lang via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Donnerstag, 11. Oktober 2018 18:58 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] VIP issue Dear all! I have a question for those, who are familiar with the VIPs from Sakura. Last time we changed the reagenses the cleaning-ethanol (96%) was very milky and even was full of many small particles. It was a paraffin-soup. What is the cause for such a case?? We have been using the organic solvent (ShellSol) for decades as xylensubstitute. Maybe the quality has suffered and the ability of solving paraffin has decreased. But are there other explanations? Maybe a malfunction of the instrument? Thanks in advance Gudrun Lang _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Fri Oct 12 12:34:29 2018 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 12 Oct 2018 13:34:29 -0400 Subject: [Histonet] We are Brainstorming on Promoting the Field of Histology and We Need Your Help! In-Reply-To: References: Message-ID: Pam Barker issues one more of her frequent appeals for histotechnologists. I can't remember when I last heard of a histotechnologist looking for a job. >From the perspective of a retired pathologist in his 80th year: we'll get enough good people in histotechnology when we start paying histotechnologists what we pay medical technologists and other health care workers with similar levels of responsibility. Nobody understands just what it is that histotechnologists - and pathologists - do. We have to change that. Bob Richmond Samurai Pathologist Maryville TN From jwwalker at rrmc.org Fri Oct 12 13:32:42 2018 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Fri, 12 Oct 2018 18:32:42 +0000 Subject: [Histonet] We are Brainstorming on Promoting the Field of Histology and We Need Your Help! In-Reply-To: References: Message-ID: Perhaps it isn't the field itself but more about the facility where histotechs are employed. In my lab the pay scale is pretty comparable between med techs, histotechs and cytotechs. We have various levels for each position that recognize additional job tasks that each level performs. It also takes strong laboratory leaders to push HR departments for market analyses to ensure pay is competitive. To me, the issue is even broader than histology. It is the whole laboratory medicine discipline. We are still the "red-headed, step child" of the medical world even though 85% of medical decisions are made based on the work we perform each day. I've been in the profession for 20 years and have seen some improvement but it is tough to compete with the draw that nursing and MD programs have on high school and early college students to those admiral professions. In my opinion, the best way is to involve high school science students with the clinical laboratory as part of experiencing and applying theory to a real world setting. We have seen some local success with this approach as our demand for lab technologists continues as our more seasoned technologists retire now that the economy is mostly better. As to pathologists, from the last numbers I saw for pathology residencies, there were lots of positions not filled 1 year ago, which is a trend I've seen for the last 5 or so years. It is a demanding training program to be sure and I've been very lucky to participate in the training of residents. I have concerns that the US will experience what our UK colleagues experienced in the 90's. My two cents, Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Bob Richmond via Histonet Sent: Friday, October 12, 2018 1:34 PM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] We are Brainstorming on Promoting the Field of Histology and We Need Your Help! Pam Barker issues one more of her frequent appeals for histotechnologists. I can't remember when I last heard of a histotechnologist looking for a job. >From the perspective of a retired pathologist in his 80th year: we'll get enough good people in histotechnology when we start paying histotechnologists what we pay medical technologists and other health care workers with similar levels of responsibility. Nobody understands just what it is that histotechnologists - and pathologists - do. We have to change that. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7C44219e131e154eb9f55b08d6306917ad%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C636749625263948390&sdata=J3vbKnncHv4l30gy8PxQOu47Vng3tvQ8adyKp5eKN0w%3D&reserved=0 From patpxs at gmail.com Mon Oct 15 12:02:52 2018 From: patpxs at gmail.com (P Sicurello) Date: Mon, 15 Oct 2018 10:02:52 -0700 Subject: [Histonet] We are Brainstorming on Promoting the Field of Histology and We Need Your Help! In-Reply-To: References: Message-ID: If we could get support for licensure in the state of California, that would help. Pathologists and CLS's have fought to keep CLIA qualified, ASCP certified HT/HTLs from being licensed in this state. Licensing increases visibility and will help increase the pay as well. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Fri, Oct 12, 2018 at 11:48 AM Joe W. Walker, Jr. via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Perhaps it isn't the field itself but more about the facility where > histotechs are employed. In my lab the pay scale is pretty comparable > between med techs, histotechs and cytotechs. We have various levels for > each position that recognize additional job tasks that each level > performs. It also takes strong laboratory leaders to push HR departments > for market analyses to ensure pay is competitive. > > To me, the issue is even broader than histology. It is the whole > laboratory medicine discipline. We are still the "red-headed, step child" > of the medical world even though 85% of medical decisions are made based on > the work we perform each day. I've been in the profession for 20 years and > have seen some improvement but it is tough to compete with the draw that > nursing and MD programs have on high school and early college students to > those admiral professions. In my opinion, the best way is to involve high > school science students with the clinical laboratory as part of > experiencing and applying theory to a real world setting. We have seen > some local success with this approach as our demand for lab technologists > continues as our more seasoned technologists retire now that the economy is > mostly better. As to pathologists, from the last numbers I saw for > pathology residencies, there were lots of positions not filled 1 year ago, > which is a trend I've seen for the last 5 or so years. It is a demanding > training program to be sure and I've been very lucky to participate in the > training of residents. I have concerns that the US will experience what > our UK colleagues experienced in the 90's. > > My two cents, > > Joe W. Walker, Jr. MS, SCT(ASCP) > Anatomical Pathology Manager > joewalker at rrmc.org, www.rrmc.org > > -----Original Message----- > From: Bob Richmond via Histonet > Sent: Friday, October 12, 2018 1:34 PM > To: Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] We are Brainstorming on Promoting the Field of > Histology and We Need Your Help! > > Pam Barker issues one more of her frequent appeals for histotechnologists. > I can't remember when I last heard of a histotechnologist looking for a > job. > > From the perspective of a retired pathologist in his 80th year: we'll get > enough good people in histotechnology when we start paying > histotechnologists what we pay medical technologists and other health care > workers with similar levels of responsibility. > > Nobody understands just what it is that histotechnologists - and > pathologists - do. We have to change that. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7C44219e131e154eb9f55b08d6306917ad%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C636749625263948390&sdata=J3vbKnncHv4l30gy8PxQOu47Vng3tvQ8adyKp5eKN0w%3D&reserved=0 > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mjdessoye at commonwealthhealth.net Mon Oct 15 12:22:06 2018 From: mjdessoye at commonwealthhealth.net (Dessoye, Michael) Date: Mon, 15 Oct 2018 17:22:06 +0000 Subject: [Histonet] TMA slides for IHC validation Message-ID: I'm using TMA slides from US Biomax to assist with IHC validation. I'm working on p120 on a Benchmark Ultra and BR2410 slides from Biomax. The cases are all invasive lobular carcinoma, which should all stain positively, but only about half are staining positive. I sent an identical slide to our reference lab and the staining matches my own. Since it's the first time I've worked with TMA slides, am I missing something obvious? Are the tissue cores generally prepared in the same fashion? Any reasons why I'm seeing inconsistent staining of tissue that should all be positive? All regular cases pulled of invasive lobular carcinoma are staining appropriately, just something unusual with these TMA slides. Any advice is appreciated! Thanks! Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | Commonwealth Health Laboratory Services | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 ********************************************************************** Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. From jmacdonald at mtsac.edu Mon Oct 15 12:29:15 2018 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Mon, 15 Oct 2018 17:29:15 +0000 Subject: [Histonet] We are Brainstorming on Promoting the Field of Histology and We Need Your Help! In-Reply-To: References: Message-ID: You have my support and I believe the support of the CSH and the NSH. -----Original Message----- From: P Sicurello via Histonet Sent: Monday, October 15, 2018 10:03 AM To: jwwalker at rrmc.org Cc: HistoNet ; Bob Richmond Subject: Re: [Histonet] We are Brainstorming on Promoting the Field of Histology and We Need Your Help! If we could get support for licensure in the state of California, that would help. Pathologists and CLS's have fought to keep CLIA qualified, ASCP certified HT/HTLs from being licensed in this state. Licensing increases visibility and will help increase the pay as well. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 From bcooper at chla.usc.edu Mon Oct 15 15:46:41 2018 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Mon, 15 Oct 2018 20:46:41 +0000 Subject: [Histonet] Low Oxygen Sensor/Alarms In-Reply-To: References: Message-ID: Hi, Posting this question again to my colleagues on Histonet, as I have yet to receive a response. In regards to newly revised LABGEN checklist, GEN.77550 states "In areas where liquid nitrogen is used, there are oxygen sensors with a low oxygen alarm mounted in an appropriate location and sufficient airflow to prevent asphyxiation." For those of you who already do this, can you please tell me the type and model of sensor you're using? Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From penny.marr at nhs.net Tue Oct 16 10:39:33 2018 From: penny.marr at nhs.net (MARR, Penelope (EAST SUSSEX HEALTHCARE NHS TRUST)) Date: Tue, 16 Oct 2018 15:39:33 +0000 Subject: [Histonet] Slide Barcode Reader Fault on Ventana BenchMark Ultras Message-ID: <35765bb5254a4fe785ee32a9f8e24af1@NH-SLPEX113.AD1.NHS.NET> Greetings All! We have 2 BenchMark Ultras that have been on-site for almost 2 years. Since we transferred our routine work onto these modules we constantly get error messages pertaining to the slide barcode reader. It often implies that there is a problem with the slide barcode. Often, if it is transferred to another drawer it will read the barcode without problem and start the run. Other times we give up and go home and when we return in the morning the module will read the barcode without issue. Sometimes just moving the slide to the other module does the trick. It is not uncommon for one run to go through without problem but when we try to add another it has a tantrum and won't read most of the slide barcodes. Changing the barcode itself often makes the situation worse. Add in the problems with faulty dispensers and we are growing to dread having to use these machines. Has anybody else had these problems? Does anyone have any possible solutions? Penny :) Penny Marr Senior BMS Histology Conquest Hospital St Leonards-on-Sea TN37 7RD penny.marr at nhs.net ******************************************************************************************************************** This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in relation to its contents. To do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland. NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and other accredited email services. For more information and to find out how you can switch, https://portal.nhs.net/help/joiningnhsmail From boznpl at aol.com Tue Oct 16 10:38:13 2018 From: boznpl at aol.com (Laurie Redmond) Date: Tue, 16 Oct 2018 15:38:13 +0000 (UTC) Subject: [Histonet] TMA slides for IHC validation References: <327092161.11478819.1539704293851.ref@mail.yahoo.com> Message-ID: <327092161.11478819.1539704293851@mail.yahoo.com> I think they usually include negative tissue in the TMA's.? Check with Biomax on your specific slides. Laurie Redmond -----Original Message----- From: Dessoye, Michael via Histonet To: histonet at lists.utsouthwestern.edu Sent: Mon, Oct 15, 2018 10:34 am Subject: [Histonet] TMA slides for IHC validation I'm using TMA slides from US Biomax to assist with IHC validation.? I'm working on p120 on a Benchmark Ultra and BR2410 slides from Biomax.? The cases are all invasive lobular carcinoma, which should all stain positively, but only about half are staining positive.? I sent an identical slide to our reference lab and the staining matches my own. Since it's the first time I've worked with TMA slides, am I missing something obvious?? Are the tissue cores generally prepared in the same fashion?? Any reasons why I'm seeing inconsistent staining of tissue that should all be positive?? All regular cases pulled of invasive lobular carcinoma are staining appropriately, just something unusual with these TMA slides. Any advice is appreciated!? Thanks! Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | Commonwealth Health Laboratory Services | mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 ********************************************************************** Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Tue Oct 16 10:49:58 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 16 Oct 2018 11:49:58 -0400 Subject: [Histonet] Get Off the Bench and Out of the Lab!!!!Histology Territory Sales Manager needed for Texas and Southwest Region. Message-ID: <04e001d46567$e42f9ef0$ac8edcd0$@earthlink.net> Hi Histonetters, How are you? I hope you are having a great week! I was hoping you might be able to help me. I am presently working with a client in need of a Marketing/Sales Representative for the state of Texas and part of the rest of the Southwest region. This is a well-known and well-liked purveyor of histology related products. My client is offering a very competitive compensation package, an excellent support network and an amazing opportunity for an experienced Rep OR someone eager to enter the field!! Histonetters, the help I need from you is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them? (remember if I place someone you refer to me you will earn a referral fee.) If you are interested in this position please contact me ASAP toll free at 866-607-3542, cell/text 407-353-5070 or via email at relia1 at earthlink.net Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From sandra.cheasty at wisc.edu Tue Oct 16 12:25:02 2018 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Tue, 16 Oct 2018 17:25:02 +0000 Subject: [Histonet] PT Module Spare Part Message-ID: Hi all, Does anyone know where I can get my hands on some PT module reagent level sensors? (The black bar on the back of each tank.) Our pH 9 AR destroyed it in one tank, and the pH 6 sensor now has some wear and tear also. Otherwise the PT module works great. Thanks! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From Theresa.Oeler at nsabp.org Tue Oct 16 13:10:30 2018 From: Theresa.Oeler at nsabp.org (Oeler, Theresa) Date: Tue, 16 Oct 2018 18:10:30 +0000 Subject: [Histonet] O2 sensors for Rooms with liquid N2. Message-ID: <816c465bba6d493aae5ad09cd2c1ef7a@nsabp.org> We have a sensor from Alpha Omega Instruments. It is VERY LOUD! But serves the need. Terry Oeler NSABP Foundation, Inc. Federal North Building 1307 Federal Street - Suite 303 Pittsburgh, PA. 15212 O # 412-697-6609 F # 412-697-6613 Theresa.oeler at nsabp.org From john.frazier at roche.com Tue Oct 16 13:30:45 2018 From: john.frazier at roche.com (Frazier, John) Date: Tue, 16 Oct 2018 14:30:45 -0400 Subject: [Histonet] We are Brainstorming on Promoting the Field of Histology and We Need Your Help! Message-ID: Please include me. I visit 20-30 labs per year as a AP workflow consultant. I have seen how the clinical side has failed to promote themselves (I am one of them, MT). We have to remember that the leaders that represent us at the national level are Pathologist (ASCP). They don?t necessarily have the promotion of Histology as a priority. I am passionate about this subject. John Frazier, MBA, MT(ASCP), LSSBB Strategic Workflow Consulting Roche Diagnotics > On Oct 15, 2018, at 1:29 PM, Mac Donald, Jennifer wrote: > > You have my support and I believe the support of the CSH and the NSH. > > -----Original Message----- > From: P Sicurello via Histonet > Sent: Monday, October 15, 2018 10:03 AM > To: jwwalker at rrmc.org > Cc: HistoNet ; Bob Richmond > Subject: Re: [Histonet] We are Brainstorming on Promoting the Field of Histology and We Need Your Help! > > If we could get support for licensure in the state of California, that would help. Pathologists and CLS's have fought to keep CLIA qualified, ASCP certified HT/HTLs from being licensed in this state. Licensing increases visibility and will help increase the pay as well. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 9300 Campus Point Drive > > La Jolla, CA 92037 > (P): 858-249-5610 > > > > From VKurth at uwhealth.org Wed Oct 17 08:56:50 2018 From: VKurth at uwhealth.org (Kurth Virginia L.) Date: Wed, 17 Oct 2018 13:56:50 +0000 Subject: [Histonet] Silver ISH Message-ID: Hello Jacqueline I am going thru old histo net messages and was wondering if you had any new insight on the silver ISH for HPV testing on the Ventana ultras, my lab would like to validate it, but I am worried about the consistency of the silver staining. Thank you, Ginny Kurth, UWHC, VKURTH at UWHEALTH.ORG From Lindsay.A.Wilson at uth.tmc.edu Wed Oct 17 14:32:41 2018 From: Lindsay.A.Wilson at uth.tmc.edu (Wilson, Lindsay A) Date: Wed, 17 Oct 2018 19:32:41 +0000 Subject: [Histonet] Superfrost Plus vs. Superfrost Plus Gold slides Message-ID: <81C817F7-9A1B-4D32-893E-2121237ECA50@uth.tmc.edu> Good afternoon, Does anyone have a preference or know the difference between the Superfrost Plus vs. Superfrost Plus Gold slides? Are the ?gold? slides worth the money? Wondering if it would help adhere decalcified FFPE bone onto slides for heat-based antigen retrieval. Currently am using Superfrost Plus slides with tissue falling off the slide. Lindsay Wilson, LVT, RLATG Young Laboratory UTHealth | The University of Texas Health Science Center at Houston | School of Dentistry Department of Oral & Maxillofacial Surgery 7500 Cambridge St. | Suite 6510 | Houston, TX 77054 713 486 4360 tel | 713 486 4333 fax From laurie.reilly at jcu.edu.au Wed Oct 17 21:51:45 2018 From: laurie.reilly at jcu.edu.au (Reilly, Laurie) Date: Thu, 18 Oct 2018 02:51:45 +0000 Subject: [Histonet] Restaining old Heamatology smears Message-ID: Dear Histonetters, This is not quite Histo but related. One of our Laboratory Scientists has removed the coverslips from faded blood smears used for teaching and is having trouble restaining them with Wright's stain. Could we please call on the combined wisdom of the Histonet to try to find a solution to this problem. Regards, Laurie. Mr. Laurie REILLY Histopathology Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 Mobile 0448 957747 From Lindsay.A.Wilson at uth.tmc.edu Thu Oct 18 11:57:01 2018 From: Lindsay.A.Wilson at uth.tmc.edu (Wilson, Lindsay A) Date: Thu, 18 Oct 2018 16:57:01 +0000 Subject: [Histonet] BMP-2 protocol for bone Message-ID: <037A611B-9C8A-4766-8109-D7A683568148@uth.tmc.edu> Good morning, Does anyone have a proven IHC protocol for BMP-2 on bone tissue? A big plus, if you have worked with it on rabbit bone! I am currently using Abcam?s BMP-2 (ab6285) with no success. I have performed antigen retrieval and non-antigen retrieval. I have used a dilution as low as 1:50. I appreciate any suggestions. Lindsay Wilson, LVT, RLATG Young Laboratory UTHealth | The University of Texas Health Science Center at Houston | School of Dentistry Department of Oral & Maxillofacial Surgery 7500 Cambridge St. | Suite 6510 | Houston, TX 77054 713 486 4360 tel | 713 486 4333 fax From m.jamison at elitechgroup.com Thu Oct 18 15:48:50 2018 From: m.jamison at elitechgroup.com (Michelle Jamison) Date: Thu, 18 Oct 2018 14:48:50 -0600 Subject: [Histonet] toluene substitute Message-ID: Hi All, I am from industry. We are interested in developing an instrument that stains then prepares microscope slides to be coverslipped. If you do coverslip, what substitute do you use instead of toluene to go to mounting media? Thank you! Michelle -- All the best to you, Michelle Jamison /Bio Research Associate / /ELITechGroup Inc. / ???370 W. 1700 S.?? ???Logan Utah ??? USA m.jamison at elitechgroup.com ? _www.elitechgroup.com _ Tel : +1.435.752.6011 Ext: 1474 Logo From erin.mccarthy at tempus.com Thu Oct 18 16:30:14 2018 From: erin.mccarthy at tempus.com (Erin McCarthy) Date: Thu, 18 Oct 2018 16:30:14 -0500 Subject: [Histonet] RUO antibody Message-ID: Hi All, I am looking to see if anyone has had any experience with the antibody AXL. We are trying to work it up on our Roche Discovery Ultra and it does not seem like it stains both nuclear and cytoplasic cell features. We only seem to see cytoplasmic staining, and in general most tumors are showing negative results. I currently am using the Ultraview kit for optimization, our pathologist likes CC2 for 44 min, Ab. Inc for 40 min using a 1:100 dilution. Anyone have any suggestions that might help? If you want more detailed info I can provide as well! -- Erin McCarthy, HT (ASCP) Pathology Lab Supervisor Tempus Labs 600 W. Chicago Ave. Chicago IL 60654 Ph:(312) 638-6344 Ext.3835 -- This email and any attachments may contain privileged and confidential information and/or protected health information (PHI) that is protected by federal and state privacy laws.? It is intended solely for the use of Tempus Labs and the recipient(s) named above.? Nothing contained in this communication and any attachments thereto is intended to waive any privileges or rights of confidentiality.? If you are not the recipient, or the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any review, dissemination, distribution, printing or copying of this email message and/or any attachments is strictly prohibited.?* If you have received this transmission in error, please notify us immediately at?**(855)-442-8305**? and permanently delete this email and any attachments*. From edmartin26 at gmail.com Thu Oct 18 17:20:53 2018 From: edmartin26 at gmail.com (Eddie Martin) Date: Thu, 18 Oct 2018 18:20:53 -0400 Subject: [Histonet] Restaining old Heamatology smears Message-ID: <0E7F124E-9821-4A32-A438-63F45AD4566E@gmail.com> Hi Laurie, Blood is considered a tissue so it is very much still Histo related. Regarding your smears, this also occurs in our hematopathology laboratory from time to time when we are scanning old slides and there?s a bubble under the coverslip that won?t let us take images with our digital scope. It may very well be that there is still a resinous permount film on your slides from the old coverslip. This may simply be removed by leaving the smear in xylene some additional time to remove the resin. Then place slides in 100% alcohol to remove xylene residues. The following steps would be rehydrating the slides with dilute changes in in alcohol...ie: 95%,then 85%, then 70%, then in a few changes of distilled water. If you can?t get the pH of your water to pH 7.2, then you could use a phosphate buffered solution to achieve this in place of the distilled water. Then place your slides in a working Giemsa working solution. It may help to rock your slides or slightly agitate them to improve staining intensity. I hope this helps. Please feel free to contact me via email at Eddie.martin at NIH.gov > On Oct 17, 2018, at 10:51 PM, Reilly, Laurie wrote: > > Dear Histonetters, > This is not quite Histo but related. One of our Laboratory Scientists has removed the coverslips from faded blood smears used for teaching and is having trouble restaining them with Wright's stain. > Could we please call on the combined wisdom of the Histonet to try to find a solution to this problem. > > Regards, Laurie. > > Mr. Laurie REILLY > Histopathology > Veterinary and Biomedical Sciences > James Cook University > Townsville Qld. 4811 > Australia. > > Phone 07 4781 4468 > Mobile 0448 957747 > > From edmartin26 at gmail.com Thu Oct 18 19:29:53 2018 From: edmartin26 at gmail.com (Eddie Martin) Date: Thu, 18 Oct 2018 20:29:53 -0400 Subject: [Histonet] BMP-2 protocol for bone Message-ID: <1F29A020-476E-4627-8E40-A4F3F146A864@gmail.com> Hi Lindsay, There are a few complications that will automatically come up due to working with rabbit tissue, and also due to the antibody from Abcam that you are using. I called Abcam prior to emailing you on a hunch that I had and was correct. The antibody you purchased from Abcam is unconjugated and first needs to be conjugated to whatever method you?re going to use. You could conjugate the antibody yourself. I already reached out to Abcam. It?s going to take them a few business days to get back to me to see whether their own antibody can be conjugated for IHC if that?s the route you?re going to go. Keeping this email simple for a response to your question, it may be easier to use another detection method for conjugating this specific antibody to rabbit bone...possibly FITC conjugated tyramide labeling with Alexa-Fluor. If you are insistent on using HRP it may be easier to contact me and we can further discuss as there are easily two complications that will arise due to the antibody you chose to optimize with and the species you are trying to stain on. Since you left your lab number on your post, I called and left my phone number on the voicemail for the young Lab if you would like to discuss and troubleshoot over the phone. I can also be reached via email at work at Eddie.martin at NIH.gov Thanks, Eddie Eddie Martin HT(ASCP), QIHC(ASCP),HTL Hematology Team Lead / Histology Technical Supervisor The National Institutes of Health Clinic Center - Department of Laboratory Medicine 10 Center Drive, Room 2C 360 Bethesda, MD 20814 > On Oct 18, 2018, at 12:57 PM, Wilson, Lindsay A wrote: > > Good morning, > > Does anyone have a proven IHC protocol for BMP-2 on bone tissue? A big plus, if you have worked with it on rabbit bone! > I am currently using Abcam?s BMP-2 (ab6285) with no success. I have performed antigen retrieval and non-antigen retrieval. I have used a dilution as low as 1:50. I appreciate any suggestions. > > Lindsay Wilson, LVT, RLATG > Young Laboratory > > UTHealth | The University of Texas Health Science Center at Houston | School of Dentistry > Department of Oral & Maxillofacial Surgery > > 7500 Cambridge St. | Suite 6510 | Houston, TX 77054 > 713 486 4360 tel | 713 486 4333 fax > > From cmmathis at wakehealth.edu Fri Oct 19 14:24:01 2018 From: cmmathis at wakehealth.edu (Cathy M. Mathis/Comparative Medicine) Date: Fri, 19 Oct 2018 19:24:01 +0000 Subject: [Histonet] TUNEL on Leica Bond Message-ID: <4816F916B11AEC4E92A0D01372F573E2012CFD315A@EXCHDB8.medctr.ad.wfubmc.edu> Hello Histo-friends, Is anyone doing a TUNEL stain on any of the Leica Bond instruments? I will be attempting to set this up and thought I would reach out first. Thank you, Cathy Cathy M. Mathis Laboratory Technician IV -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Friday, October 19, 2018 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 179, Issue 16 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w&r=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg&m=HlJCIs0uAvNmmGWdd-pEFIDvdEBtEL2uYCMe-3yHqVk&s=19Ow0ZV3sn97Uww-nCnsPHdxQtOFWY-zJR4M1RFnuE8&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. toluene substitute (Michelle Jamison) 2. RUO antibody (Erin McCarthy) 3. Re: Restaining old Heamatology smears (Eddie Martin) 4. Re: BMP-2 protocol for bone (Eddie Martin) ---------------------------------------------------------------------- Message: 1 Date: Thu, 18 Oct 2018 14:48:50 -0600 From: Michelle Jamison To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] toluene substitute Message-ID: Content-Type: text/plain; charset=utf-8; format=flowed Hi All, I am from industry. We are interested in developing an instrument that stains then prepares microscope slides to be coverslipped. If you do coverslip, what substitute do you use instead of toluene to go to mounting media? Thank you! Michelle -- All the best to you, Michelle Jamison /Bio Research Associate / /ELITechGroup Inc. / ???370 W. 1700 S.?? ???Logan Utah ??? USA m.jamison at elitechgroup.com ? _https://urldefense.proofpoint.com/v2/url?u=http-3A__www.elitechgroup.com&d=DwICAg&c=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w&r=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg&m=HlJCIs0uAvNmmGWdd-pEFIDvdEBtEL2uYCMe-3yHqVk&s=zEZUJnDPDmAli_gIL21jiQLLLktKgspCpja5mtUKhpg&e= _ Tel : +1.435.752.6011 Ext: 1474 Logo ------------------------------ Message: 2 Date: Thu, 18 Oct 2018 16:30:14 -0500 From: Erin McCarthy To: histonet at lists.utsouthwestern.edu Subject: [Histonet] RUO antibody Message-ID: Content-Type: text/plain; charset="UTF-8" Hi All, I am looking to see if anyone has had any experience with the antibody AXL. We are trying to work it up on our Roche Discovery Ultra and it does not seem like it stains both nuclear and cytoplasic cell features. We only seem to see cytoplasmic staining, and in general most tumors are showing negative results. I currently am using the Ultraview kit for optimization, our pathologist likes CC2 for 44 min, Ab. Inc for 40 min using a 1:100 dilution. Anyone have any suggestions that might help? If you want more detailed info I can provide as well! -- Erin McCarthy, HT (ASCP) Pathology Lab Supervisor Tempus Labs 600 W. Chicago Ave. Chicago IL 60654 Ph:(312) 638-6344 Ext.3835 -- This email and any attachments may contain privileged and confidential information and/or protected health information (PHI) that is protected by federal and state privacy laws.? It is intended solely for the use of Tempus Labs and the recipient(s) named above.? Nothing contained in this communication and any attachments thereto is intended to waive any privileges or rights of confidentiality.? If you are not the recipient, or the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any review, dissemination, distribution, printing or copying of this email message and/or any attachments is strictly prohibited.?* If you have received this transmission in error, please notify us immediately at?**(855)-442-8305**? and permanently delete this email and any attachments*. ------------------------------ Message: 3 Date: Thu, 18 Oct 2018 18:20:53 -0400 From: Eddie Martin To: "Reilly, Laurie" Cc: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Restaining old Heamatology smears Message-ID: <0E7F124E-9821-4A32-A438-63F45AD4566E at gmail.com> Content-Type: text/plain; charset=utf-8 Hi Laurie, Blood is considered a tissue so it is very much still Histo related. Regarding your smears, this also occurs in our hematopathology laboratory from time to time when we are scanning old slides and there?s a bubble under the coverslip that won?t let us take images with our digital scope. It may very well be that there is still a resinous permount film on your slides from the old coverslip. This may simply be removed by leaving the smear in xylene some additional time to remove the resin. Then place slides in 100% alcohol to remove xylene residues. The following steps would be rehydrating the slides with dilute changes in in alcohol...ie: 95%,then 85%, then 70%, then in a few changes of distilled water. If you can?t get the pH of your water to pH 7.2, then you could use a phosphate buffered solution to achieve this in place of the distilled water. Then place your slides in a working Giemsa working solution. It may help to rock your slides or slightly agitate them to improve staining intensity. I hope this helps. Please feel free to contact me via email at Eddie.martin at NIH.gov > On Oct 17, 2018, at 10:51 PM, Reilly, Laurie wrote: > > Dear Histonetters, > This is not quite Histo but related. One of our Laboratory Scientists has removed the coverslips from faded blood smears used for teaching and is having trouble restaining them with Wright's stain. > Could we please call on the combined wisdom of the Histonet to try to find a solution to this problem. > > Regards, Laurie. > > Mr. Laurie REILLY > Histopathology > Veterinary and Biomedical Sciences > James Cook University > Townsville Qld. 4811 > Australia. > > Phone 07 4781 4468 > Mobile 0448 957747 > > ------------------------------ Message: 4 Date: Thu, 18 Oct 2018 20:29:53 -0400 From: Eddie Martin To: "Wilson, Lindsay A" Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] BMP-2 protocol for bone Message-ID: <1F29A020-476E-4627-8E40-A4F3F146A864 at gmail.com> Content-Type: text/plain; charset=utf-8 Hi Lindsay, There are a few complications that will automatically come up due to working with rabbit tissue, and also due to the antibody from Abcam that you are using. I called Abcam prior to emailing you on a hunch that I had and was correct. The antibody you purchased from Abcam is unconjugated and first needs to be conjugated to whatever method you?re going to use. You could conjugate the antibody yourself. I already reached out to Abcam. It?s going to take them a few business days to get back to me to see whether their own antibody can be conjugated for IHC if that?s the route you?re going to go. Keeping this email simple for a response to your question, it may be easier to use another detection method for conjugating this specific antibody to rabbit bone...possibly FITC conjugated tyramide labeling with Alexa-Fluor. If you are insistent on using HRP it may be easier to contact me and we can further discuss as there are easily two complications that will arise due to the antibody you chose to optimize with and the species you are trying to stain on. Since you left your lab number on your post, I called and left my phone number on the voicemail for the young Lab if you would like to discuss and troubleshoot over the phone. I can also be reached via email at work at Eddie.martin at NIH.gov Thanks, Eddie Eddie Martin HT(ASCP), QIHC(ASCP),HTL Hematology Team Lead / Histology Technical Supervisor The National Institutes of Health Clinic Center - Department of Laboratory Medicine 10 Center Drive, Room 2C 360 Bethesda, MD 20814 > On Oct 18, 2018, at 12:57 PM, Wilson, Lindsay A wrote: > > Good morning, > > Does anyone have a proven IHC protocol for BMP-2 on bone tissue? A big plus, if you have worked with it on rabbit bone! > I am currently using Abcam?s BMP-2 (ab6285) with no success. I have performed antigen retrieval and non-antigen retrieval. I have used a dilution as low as 1:50. I appreciate any suggestions. > > Lindsay Wilson, LVT, RLATG > Young Laboratory > > UTHealth | The University of Texas Health Science Center at Houston | School of Dentistry > Department of Oral & Maxillofacial Surgery > > 7500 Cambridge St. | Suite 6510 | Houston, TX 77054 > 713 486 4360 tel | 713 486 4333 fax > > ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w&r=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg&m=HlJCIs0uAvNmmGWdd-pEFIDvdEBtEL2uYCMe-3yHqVk&s=19Ow0ZV3sn97Uww-nCnsPHdxQtOFWY-zJR4M1RFnuE8&e= ------------------------------ End of Histonet Digest, Vol 179, Issue 16 ***************************************** From amosbrooks at gmail.com Sun Oct 21 17:54:46 2018 From: amosbrooks at gmail.com (Amos Brooks) Date: Sun, 21 Oct 2018 18:54:46 -0400 Subject: [Histonet] Tunel Message-ID: Hi, > I hand stain this. It is a finicky test and benefits from a personal touch. When I have a ton of them, sometimes I do the enzyme on the stainer and then take it off for the primary and secondary then DAB on the stainer. Amos Message: 1 Date: Fri, 19 Oct 2018 19:24:01 +0000 From: "Cathy M. Mathis/Comparative Medicine" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] TUNEL on Leica Bond Message-ID: < 4816F916B11AEC4E92A0D01372F573E2012CFD315A at EXCHDB8.medctr.ad.wfubmc.edu> Content-Type: text/plain; charset="us-ascii" Hello Histo-friends, Is anyone doing a TUNEL stain on any of the Leica Bond instruments? I will be attempting to set this up and thought I would reach out first. Thank you, Cathy Cathy M. Mathis Laboratory Technician IV From VKurth at uwhealth.org Mon Oct 22 14:11:30 2018 From: VKurth at uwhealth.org (Kurth Virginia L.) Date: Mon, 22 Oct 2018 19:11:30 +0000 Subject: [Histonet] HPV ISH Message-ID: Hello I have seen question for KDL asking about HPV ISH (awhile back). My lab would like to bring on the HPV ISH using the RNA ISH probes provided by Ventana using the silver detection, are you on board using this process for HPV, do you have any insight for me. Thanks! Ginny, UWHC-Wisconsin, vkurth at uwhealth.org From relia1 at earthlink.net Tue Oct 23 14:45:31 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 23 Oct 2018 15:45:31 -0400 Subject: [Histonet] Enjoying the Fall Season? Message-ID: <000001d46b08$f5472d60$dfd58820$@earthlink.net> Hello Histonetters, How are you? Are you enjoying the sights, sounds and flavors of Fall? I know I am enjoying college football after all I am a southern gal and you know how we southerners are about our Football Saturdays in the South. On the other hand I am still patiently waiting for that ?real Fall stuff?. You know like crisp cool air, beautiful fall colors, crunching leaves and steaming bowls of soup. As it still ?feels? like Summer here in Florida! What are you enjoying most of all about this pumpkin spice flavored season? Histonetters, here is a list of my current openings. Please take a look and see if anything interests you or if you can think of anyone you know that might be interested in a new opportunity! Remember if I hire someone you refer to me you will earn a referral bonus!! Here are my Management Opportunities: Histology Lab Manager Fayetteville, NC Senior Histology Tech Norfolk, VA Histology Sales Manager Dallas, TX Here are my HT/HTL and eligible positions: ? Texas Histology Sales Rep ? North Carolina Histology Tech ? Days!! Relo! ? Massachusetts Dermpath Histotech Days!!! Relo! ? Virginia Histotech Relo and sign on bonus! If you have another area in mind please drop me an email and let me know so that I can be on the lookout! I spend all day everyday looking at new job opportunities so you don?t have to! If you or anyone you know might be interested in any of these opportunities or would like help with a job search in another area of the USA please contact me. I can be reached ASAP on my cell or leave me a message to set up an appointment to chat at your convenience at relia1 at earthlink.net or toll free at 866-607-3542. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From criley at dpspa.com Tue Oct 23 17:16:54 2018 From: criley at dpspa.com (Charles Riley) Date: Tue, 23 Oct 2018 18:16:54 -0400 Subject: [Histonet] Cost calculations Message-ID: How does everyone calculate their cost to create things in your lab? I was tasked to figure out how much it costs us to make the following 1. A paraffin block 2. A single H&E slide 3. Average cost of an IHC slide 4. Average cost of a Special stain slide What I need to figure out is beside the cost on the inventory to create these items what other factors do I need/should use to calculate final costs (i.e. tech time, energy costs, machine maintenance....)? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From rjbuesa at yahoo.com Wed Oct 24 08:42:35 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Wed, 24 Oct 2018 13:42:35 +0000 (UTC) Subject: [Histonet] Cost calculations In-Reply-To: References: Message-ID: <725530768.120686.1540388555214@mail.yahoo.com> Charles:There is the "easy way" and the "hard way" and the differences are minuscule:1- add what cost you ALL the ingredients for each aspect you want to determine2- add the salary of the people doing the task related to productivity (blocks or slides or whatever per hour)3- add-up 1 + 24- divide by the number of "units" produced.Under separate cover I am sending an article I wrote on the subject Ren? On Tuesday, October 23, 2018 6:26 PM, Charles Riley via Histonet wrote: How does everyone calculate their cost to create things in your lab? I was tasked to figure out how much it costs us to make the following 1. A paraffin block 2. A single H&E slide 3. Average cost of an IHC slide 4. Average cost of a Special stain slide What I need to figure out is beside the cost on the inventory to create these items what other factors do I need/should use to calculate final costs (i.e. tech time, energy costs, machine maintenance....)? -- Charles Riley BS? HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed Oct 24 11:04:18 2018 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 24 Oct 2018 12:04:18 -0400 Subject: [Histonet] Enjoying the Fall Season? Message-ID: <009801d46bb3$387fbac0$a97f3040$@earthlink.net> Hello Histonetters, How are you? Are you enjoying the sights, sounds and flavors of Fall? I know I am enjoying college football after all I am a southern gal and you know how we southerners are about our Football Saturdays in the South. On the other hand I am still patiently waiting for that ?real Fall stuff?. You know like crisp cool air, beautiful fall colors, crunching leaves and steaming bowls of soup. As it still ?feels? like Summer here in Florida! What are you enjoying most of all about this pumpkin spice flavored season? Histonetters, here is a list of my current openings. Please take a look and see if anything interests you or if you can think of anyone you know that might be interested in a new opportunity! Remember if I hire someone you refer to me you will earn a referral bonus!! Here are my Management Opportunities: Histology Lab Manager Fayetteville, NC Senior Histology Tech Norfolk, VA Histology Sales Manager Dallas, TX Here are my HT/HTL and eligible positions: ? Texas Histology Sales Rep ? North Carolina Histology Tech ? Days!! Relo! ? Massachusetts Dermpath Histotech Days!!! Relo! ? Virginia Histotech Relo and sign on bonus! If you have another area in mind please drop me an email and let me know so that I can be on the lookout! I spend all day everyday looking at new job opportunities so you don?t have to! If you or anyone you know might be interested in any of these opportunities or would like help with a job search in another area of the USA please contact me. I can be reached ASAP on my cell or leave me a message to set up an appointment to chat at your convenience at relia1 at earthlink.net or toll free at 866-607-3542. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From linda at imebinc.com Wed Oct 24 16:56:53 2018 From: linda at imebinc.com (Linda Miller) Date: Wed, 24 Oct 2018 14:56:53 -0700 Subject: [Histonet] Bouin's Fixative Substitute Message-ID: <028101d46be4$79980b30$6cc82190$@imebinc.com> New! Bouin's Fixative Substitute is a low-hazard replacement for Bouin's Fixative. This substitute provides all the benefits of Bouin's without the use of picric acid. Available in a variety of sizes and pre-filled specimen containers. Available at IMEB, Inc. -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Friday, October 19, 2018 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 179, Issue 16 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. toluene substitute (Michelle Jamison) 2. RUO antibody (Erin McCarthy) 3. Re: Restaining old Heamatology smears (Eddie Martin) 4. Re: BMP-2 protocol for bone (Eddie Martin) ---------------------------------------------------------------------- Message: 1 Date: Thu, 18 Oct 2018 14:48:50 -0600 From: Michelle Jamison To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] toluene substitute Message-ID: Content-Type: text/plain; charset=utf-8; format=flowed Hi All, I am from industry. We are interested in developing an instrument that stains then prepares microscope slides to be coverslipped. If you do coverslip, what substitute do you use instead of toluene to go to mounting media? Thank you! Michelle -- All the best to you, Michelle Jamison /Bio Research Associate / /ELITechGroup Inc. / ???370 W. 1700 S.?? ???Logan Utah ??? USA m.jamison at elitechgroup.com ? _www.elitechgroup.com _ Tel : +1.435.752.6011 Ext: 1474 Logo ------------------------------ Message: 2 Date: Thu, 18 Oct 2018 16:30:14 -0500 From: Erin McCarthy To: histonet at lists.utsouthwestern.edu Subject: [Histonet] RUO antibody Message-ID: Content-Type: text/plain; charset="UTF-8" Hi All, I am looking to see if anyone has had any experience with the antibody AXL. We are trying to work it up on our Roche Discovery Ultra and it does not seem like it stains both nuclear and cytoplasic cell features. We only seem to see cytoplasmic staining, and in general most tumors are showing negative results. I currently am using the Ultraview kit for optimization, our pathologist likes CC2 for 44 min, Ab. Inc for 40 min using a 1:100 dilution. Anyone have any suggestions that might help? If you want more detailed info I can provide as well! -- Erin McCarthy, HT (ASCP) Pathology Lab Supervisor Tempus Labs 600 W. Chicago Ave. Chicago IL 60654 Ph:(312) 638-6344 Ext.3835 -- This email and any attachments may contain privileged and confidential information and/or protected health information (PHI) that is protected by federal and state privacy laws.? It is intended solely for the use of Tempus Labs and the recipient(s) named above.? Nothing contained in this communication and any attachments thereto is intended to waive any privileges or rights of confidentiality.? If you are not the recipient, or the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any review, dissemination, distribution, printing or copying of this email message and/or any attachments is strictly prohibited.?* If you have received this transmission in error, please notify us immediately at?**(855)-442-8305**? and permanently delete this email and any attachments*. ------------------------------ Message: 3 Date: Thu, 18 Oct 2018 18:20:53 -0400 From: Eddie Martin To: "Reilly, Laurie" Cc: "Histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Restaining old Heamatology smears Message-ID: <0E7F124E-9821-4A32-A438-63F45AD4566E at gmail.com> Content-Type: text/plain; charset=utf-8 Hi Laurie, Blood is considered a tissue so it is very much still Histo related. Regarding your smears, this also occurs in our hematopathology laboratory from time to time when we are scanning old slides and there?s a bubble under the coverslip that won?t let us take images with our digital scope. It may very well be that there is still a resinous permount film on your slides from the old coverslip. This may simply be removed by leaving the smear in xylene some additional time to remove the resin. Then place slides in 100% alcohol to remove xylene residues. The following steps would be rehydrating the slides with dilute changes in in alcohol...ie: 95%,then 85%, then 70%, then in a few changes of distilled water. If you can?t get the pH of your water to pH 7.2, then you could use a phosphate buffered solution to achieve this in place of the distilled water. Then place your slides in a working Giemsa working solution. It may help to rock your slides or slightly agitate them to improve staining intensity. I hope this helps. Please feel free to contact me via email at Eddie.martin at NIH.gov > On Oct 17, 2018, at 10:51 PM, Reilly, Laurie wrote: > > Dear Histonetters, > This is not quite Histo but related. One of our Laboratory Scientists has removed the coverslips from faded blood smears used for teaching and is having trouble restaining them with Wright's stain. > Could we please call on the combined wisdom of the Histonet to try to find a solution to this problem. > > Regards, Laurie. > > Mr. Laurie REILLY > Histopathology > Veterinary and Biomedical Sciences > James Cook University > Townsville Qld. 4811 > Australia. > > Phone 07 4781 4468 > Mobile 0448 957747 > > ------------------------------ Message: 4 Date: Thu, 18 Oct 2018 20:29:53 -0400 From: Eddie Martin To: "Wilson, Lindsay A" Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] BMP-2 protocol for bone Message-ID: <1F29A020-476E-4627-8E40-A4F3F146A864 at gmail.com> Content-Type: text/plain; charset=utf-8 Hi Lindsay, There are a few complications that will automatically come up due to working with rabbit tissue, and also due to the antibody from Abcam that you are using. I called Abcam prior to emailing you on a hunch that I had and was correct. The antibody you purchased from Abcam is unconjugated and first needs to be conjugated to whatever method you?re going to use. You could conjugate the antibody yourself. I already reached out to Abcam. It?s going to take them a few business days to get back to me to see whether their own antibody can be conjugated for IHC if that?s the route you?re going to go. Keeping this email simple for a response to your question, it may be easier to use another detection method for conjugating this specific antibody to rabbit bone...possibly FITC conjugated tyramide labeling with Alexa-Fluor. If you are insistent on using HRP it may be easier to contact me and we can further discuss as there are easily two complications that will arise due to the antibody you chose to optimize with and the species you are trying to stain on. Since you left your lab number on your post, I called and left my phone number on the voicemail for the young Lab if you would like to discuss and troubleshoot over the phone. I can also be reached via email at work at Eddie.martin at NIH.gov Thanks, Eddie Eddie Martin HT(ASCP), QIHC(ASCP),HTL Hematology Team Lead / Histology Technical Supervisor The National Institutes of Health Clinic Center - Department of Laboratory Medicine 10 Center Drive, Room 2C 360 Bethesda, MD 20814 > On Oct 18, 2018, at 12:57 PM, Wilson, Lindsay A wrote: > > Good morning, > > Does anyone have a proven IHC protocol for BMP-2 on bone tissue? A big plus, if you have worked with it on rabbit bone! > I am currently using Abcam?s BMP-2 (ab6285) with no success. I have performed antigen retrieval and non-antigen retrieval. I have used a dilution as low as 1:50. I appreciate any suggestions. > > Lindsay Wilson, LVT, RLATG > Young Laboratory > > UTHealth | The University of Texas Health Science Center at Houston | School of Dentistry > Department of Oral & Maxillofacial Surgery > > 7500 Cambridge St. | Suite 6510 | Houston, TX 77054 > 713 486 4360 tel | 713 486 4333 fax > > ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 179, Issue 16 ***************************************** From rjbuesa at yahoo.com Thu Oct 25 09:03:28 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 25 Oct 2018 14:03:28 +0000 (UTC) Subject: [Histonet] Bouin's Fixative Substitute In-Reply-To: <028101d46be4$79980b30$6cc82190$@imebinc.com> References: <028101d46be4$79980b30$6cc82190$@imebinc.com> Message-ID: <1760478333.204088.1540476208097@mail.yahoo.com> If "New Bouin" has no pricric acid it is NOT Bouin. You can call it whatever you want but it is not Bouin nor it can have the "quality" provided by picric acid without picric acid.What you wrote is simply a deceiving"sales pitch".Ren? On Wednesday, October 24, 2018 6:10 PM, Linda Miller via Histonet wrote: New! Bouin's Fixative Substitute is a low-hazard replacement for Bouin's Fixative.? This substitute provides all the benefits of Bouin's without the use of picric acid. Available in a variety of sizes and pre-filled specimen containers. Available at IMEB, Inc. -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Friday, October 19, 2018 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 179, Issue 16 Send Histonet mailing list submissions to ??? histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. toluene substitute (Michelle Jamison) ? 2. RUO antibody (Erin McCarthy) ? 3. Re: Restaining old Heamatology smears (Eddie Martin) ? 4. Re: BMP-2 protocol for bone (Eddie Martin) ---------------------------------------------------------------------- Message: 1 Date: Thu, 18 Oct 2018 14:48:50 -0600 From: Michelle Jamison To: "histonet at lists.utsouthwestern.edu" ??? Subject: [Histonet] toluene substitute Message-ID: Content-Type: text/plain; charset=utf-8; format=flowed Hi All, I am from industry. We are interested in developing an instrument that stains then prepares microscope slides to be coverslipped. If you do coverslip, what substitute do you use instead of toluene to go to mounting media? Thank you! Michelle -- All the best to you, Michelle Jamison /Bio Research Associate / /ELITechGroup Inc. / ???370 W. 1700 S.?? ???Logan Utah ??? USA m.jamison at elitechgroup.com ? _www.elitechgroup.com _ Tel : +1.435.752.6011 Ext: 1474 Logo ------------------------------ Message: 2 Date: Thu, 18 Oct 2018 16:30:14 -0500 From: Erin McCarthy To: histonet at lists.utsouthwestern.edu Subject: [Histonet] RUO antibody Message-ID: ??? Content-Type: text/plain; charset="UTF-8" Hi All, I am looking to see if anyone has had any experience with the antibody AXL. We are trying to work it up on our Roche Discovery Ultra and it does not seem like it stains both nuclear and cytoplasic cell features. We only seem to see cytoplasmic staining, and in general most tumors are showing negative results. I currently am using the Ultraview kit for optimization, our pathologist likes CC2 for 44 min, Ab. Inc for 40 min using a 1:100 dilution. Anyone have any suggestions that might help? If you want more detailed info I can provide as well! -- Erin McCarthy, HT (ASCP) Pathology Lab Supervisor Tempus Labs 600 W. Chicago Ave. Chicago IL 60654 Ph:(312) 638-6344 Ext.3835 -- This email and any attachments may contain privileged and confidential information and/or protected health information (PHI) that is protected by federal and state privacy laws.? It is intended solely for the use of Tempus Labs and the recipient(s) named above.? Nothing contained in this communication and any attachments thereto is intended to waive any privileges or rights of confidentiality.? If you are not the recipient, or the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any review, dissemination, distribution, printing or copying of this email message and/or any attachments is strictly prohibited.?* If you have received this transmission in error, please notify us immediately at?**(855)-442-8305**? and permanently delete this email and any attachments*. ------------------------------ Message: 3 Date: Thu, 18 Oct 2018 18:20:53 -0400 From: Eddie Martin To: "Reilly, Laurie" Cc: "Histonet at lists.utsouthwestern.edu" ??? Subject: Re: [Histonet] Restaining old Heamatology smears Message-ID: <0E7F124E-9821-4A32-A438-63F45AD4566E at gmail.com> Content-Type: text/plain;??? charset=utf-8 Hi Laurie, Blood is considered a tissue so it is very much still Histo related. Regarding your smears, this also occurs in our hematopathology laboratory from time to time when we are scanning old slides and there?s a bubble under the coverslip that won?t let us take images with our digital scope. It may very well be that there is still a resinous permount film on your slides from the old coverslip. This may simply be removed by leaving the smear in xylene some additional time to remove the resin. Then place slides in 100% alcohol to remove xylene residues. The following steps would be rehydrating the slides with dilute changes in in alcohol...ie: 95%,then 85%, then 70%, then in a few changes of distilled water. If you can?t get the pH of your water to pH 7.2, then you could use a phosphate buffered solution to achieve this in place of the distilled water. Then place your slides in a working Giemsa working? solution. It may help to rock your slides or slightly agitate them to improve staining intensity. I hope this helps. Please feel free to contact me via email at Eddie.martin at NIH.gov > On Oct 17, 2018, at 10:51 PM, Reilly, Laurie wrote: > > Dear Histonetters, > This is not quite Histo but related. One of our Laboratory Scientists has removed the coverslips from faded blood smears used for teaching and is having trouble restaining them with Wright's stain. > Could we please call on the combined wisdom of the Histonet to try to find a solution to this problem. > > Regards,? ? ? Laurie. > > Mr. Laurie REILLY > Histopathology > Veterinary and Biomedical Sciences > James Cook University > Townsville? Qld.? 4811 > Australia. > > Phone 07 4781 4468 > Mobile 0448 957747 > > ------------------------------ Message: 4 Date: Thu, 18 Oct 2018 20:29:53 -0400 From: Eddie Martin To: "Wilson, Lindsay A" Cc: "histonet at lists.utsouthwestern.edu" ??? Subject: Re: [Histonet] BMP-2 protocol for bone Message-ID: <1F29A020-476E-4627-8E40-A4F3F146A864 at gmail.com> Content-Type: text/plain;??? charset=utf-8 Hi Lindsay, There are a few complications? that will automatically come up due to working with rabbit tissue, and also due to the antibody from Abcam that you are using. I called Abcam prior to emailing you on a hunch that I had and was correct. The antibody you purchased from Abcam is unconjugated and first needs to be conjugated to whatever method you?re going to use. You could conjugate the antibody yourself. I already reached out to Abcam. It?s going to take them a few business days to get back to me to see whether their own antibody can be conjugated for IHC if that?s the route you?re going to go. Keeping this email simple for a response to your question, it may be easier to use another detection method for conjugating this specific antibody to rabbit bone...possibly FITC conjugated tyramide labeling with Alexa-Fluor. If you are insistent on using HRP it may be easier to contact me and we can further discuss as there are easily two complications that will arise due to the antibody you chose to optimize with and the species you are trying to stain on. Since you left your lab number on your post, I called and left my phone number on the voicemail for the young Lab if you would like to discuss and troubleshoot over the phone. I can also be reached via email at work at Eddie.martin at NIH.gov Thanks, Eddie Eddie Martin HT(ASCP), QIHC(ASCP),HTL Hematology Team Lead / Histology Technical Supervisor The National Institutes of Health Clinic Center - Department of Laboratory Medicine 10 Center Drive, Room 2C 360 Bethesda, MD 20814 > On Oct 18, 2018, at 12:57 PM, Wilson, Lindsay A wrote: > > Good morning, > > Does anyone have a proven IHC protocol for BMP-2 on bone tissue? A big plus, if you have worked with it on rabbit bone! > I am currently using Abcam?s BMP-2 (ab6285) with no success. I have performed antigen retrieval and non-antigen retrieval. I have used a dilution as low as 1:50. I appreciate any suggestions. > > Lindsay Wilson, LVT, RLATG > Young Laboratory > > UTHealth | The University of Texas Health Science Center at Houston | School of Dentistry > Department of Oral & Maxillofacial Surgery > > 7500 Cambridge St. | Suite 6510 | Houston, TX 77054 > 713 486 4360 tel | 713 486 4333 fax > > ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 179, Issue 16 ***************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victoriagp40 at hotmail.com Thu Oct 25 12:04:56 2018 From: victoriagp40 at hotmail.com (=?utf-8?B?dmljdG9yaWEgZ2FyY8OtYSBwYWxhY2lvcw==?=) Date: Thu, 25 Oct 2018 17:04:56 +0000 Subject: [Histonet] Please : I don, t want to receive e-mail. I am very busy. Thank you Message-ID: Enviado desde mi Huawei From rsrichmond at gmail.com Thu Oct 25 13:16:27 2018 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 25 Oct 2018 14:16:27 -0400 Subject: [Histonet] Bouin's Fixative Substitute In-Reply-To: References: Message-ID: Linda Miller posts in what appears to be an advertisement: >>New! Bouin's Fixative Substitute is a low-hazard replacement for Bouin's Fixative. This substitute provides all the benefits of Bouin's without the use of picric acid. Available in a variety of sizes and pre-filled specimen containers.<< It seems to me that "Bouin's Fixative Substitute" is an honest name for the stuff. But what's in it that substitutes for picric acid? If that's a trade secret, then I wouldn't use the stuff. If you tell us, I'm sure many of us will be happy to buy it rather than brew it, but secret formulas have no place in science. Bob Richmond Samurai Pathologist Maryville TN From Timothy.Morken at ucsf.edu Thu Oct 25 13:15:35 2018 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 25 Oct 2018 18:15:35 +0000 Subject: [Histonet] Frozen sections and cold acetone... Message-ID: Can anyone give me a rational for using cold (refrig or freezer-temp) acetone to fix frozen sections? Or a rational for using RT acetone. This is for kidney or muscle bx frozens for immmunofluroescence or immunoperoxidase staining. Normally they air dry for at least 15 minutes (just waiting for frozen sectioning to be completed) before going into acetone. Just wondering if we can reduce complexity... I haven't seen anything saying why cold acetone is used, just instructions to do so. I always wonder about such things... Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From llewllew at shaw.ca Thu Oct 25 13:54:04 2018 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Thu, 25 Oct 2018 11:54:04 -0700 Subject: [Histonet] Frozen sections and cold acetone... In-Reply-To: References: Message-ID: <7b25693f-e20f-67ca-fad7-dee05adee791@shaw.ca> Cold acetone, and cold ethanol, were used to fix tissues because they left enzymes unaffected and still demonstrable. This was in the early days of enzyme histochemistry. Pearse' Histochemistry: Theoretical and applied,3rd edition, volume 1, page 85 discusses it. I could send a scan if you wanted. Bryan Llewellyn Morken, Timothy via Histonet wrote: > Can anyone give me a rational for using cold (refrig or freezer-temp) acetone to fix frozen sections? Or a rational for using RT acetone. > > This is for kidney or muscle bx frozens for immmunofluroescence or immunoperoxidase staining. > > Normally they air dry for at least 15 minutes (just waiting for frozen sectioning to be completed) before going into acetone. Just wondering if we can reduce complexity... > > I haven't seen anything saying why cold acetone is used, just instructions to do so. I always wonder about such things... > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Richard.Cartun at hhchealth.org Thu Oct 25 17:01:31 2018 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Thu, 25 Oct 2018 22:01:31 +0000 Subject: [Histonet] Alizarin Red Stain Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EA8A9AE55@HHCEXCHMB03.hhcsystem.org> Does anyone do the Alizarin Red histochemical stain for calcium in clinical samples? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From tony.henwood at health.nsw.gov.au Thu Oct 25 17:34:36 2018 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 25 Oct 2018 22:34:36 +0000 Subject: [Histonet] Alizarin Red Stain In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2EA8A9AE55@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2EA8A9AE55@HHCEXCHMB03.hhcsystem.org> Message-ID: Hi Richard, We sure do Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Cartun, Richard via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 26 October 2018 9:02 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Alizarin Red Stain Does anyone do the Alizarin Red histochemical stain for calcium in clinical samples? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From tony.henwood at health.nsw.gov.au Thu Oct 25 18:51:45 2018 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 25 Oct 2018 23:51:45 +0000 Subject: [Histonet] Frozen sections and cold acetone... In-Reply-To: <7b25693f-e20f-67ca-fad7-dee05adee791@shaw.ca> References: <7b25693f-e20f-67ca-fad7-dee05adee791@shaw.ca> Message-ID: <23e7514abddf4a31a2a060366a3c7c3d@SVDCMBX-MEX024.nswhealth.net> Hi Tim (and Bryan), This is from a book chapter I wrote (though not published as yet), that might be useful: "It is important that fixation has minimal effect on antigenic determinates, allowing recognition by appropriate antibodies. Fixatives can be physical or chemical in action. Physical fixatives, for example heating sections or cells on to slides, alcohol or acetone treatment, preserve tissues by dehydrating cells. Alcohol and acetone possibly also dissolve lipids from cell membranes facilitating antibody access." And "With today's modern immunohistochemical methods being done quite successfully on formalin-fixed, paraffin-embedded sections, it is mystifying as to why immunofluorescence is still preferred for immunopathological renal and skin diagnosis. Immunofluorescence remains the most popular method of detecting immunoglobulins and complement deposits, with 83% of British laboratories using this technique (36). Technically, frozen sections are cut, dried, rinsed in buffer, optimally diluted FITC labelled antibodies are added to sections, incubated for 30-60 minutes, non-bound antibody is rinsed from the sections with buffer, the sections are coverslipped with a non-fluorescing aqueous mountant and finally viewed using a microscope equipped with an ultra-violet light source; overall quite a simple technique." "The detection of tissue-bound autoantibodies is rendered difficult by the presence of abundant plasma proteins, which must be removed to avoid non-specific staining. This has resulted in a continuing popularity of frozen sections and immunofluorescence techniques in immunopathology, where they have been superseded in most other branches of immunohistochemistry by methods that are applicable to paraffin wax sections. In paraffin wax sections, the plasma proteins have been fixed in the tissues and must be removed by a digestion process rather than simple washing (37). Unfortunately, too much digestion may destroy tissue integrity and possibly remove the immune complexes, resulting in a false negative result. Furthermore, digestion conditions are difficult to standardize. The times required vary with the degree of fixation and section thickness (38). Frozen sections of non-fixed tissue, when used for the detection of bound-immune complexes, give clean localization with minimal background without the requirement for enzymatic pre-digestion. The plasma immunoglobulins, being loosely bound, if bound at all, to the surrounding tissue elements are easily dissolved in the warm buffer pre-incubation step. Bound immune-complexes seem to be unaffected." 36. Furness PN & Boyd S (1996). Electron microscopy and immunocytochemistry in the assessment of renal biopsy specimens: actual and optimal practice. Journal of clinical pathology, 49(3):233-237 37. Furness PN (2000). ACP Best practice No 160. Renal biopsy specimens. Journal of clinical pathology, 53(6):433-438. 38. Lehman JS & Camilleri MJ (2013). Potential diagnostic pitfall in direct immunofluorescence evaluation for vasculitis in skin biopsies: fluorescent secretory granules within eccrine glands. Journal of cutaneous pathology 40:603-605 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Bryan Llewellyn via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 26 October 2018 5:54 AM To: Histonet Subject: Re: [Histonet] Frozen sections and cold acetone... Cold acetone, and cold ethanol, were used to fix tissues because they left enzymes unaffected and still demonstrable. This was in the early days of enzyme histochemistry. Pearse' Histochemistry: Theoretical and applied,3rd edition, volume 1, page 85 discusses it. I could send a scan if you wanted. Bryan Llewellyn Morken, Timothy via Histonet wrote: > Can anyone give me a rational for using cold (refrig or freezer-temp) acetone to fix frozen sections? Or a rational for using RT acetone. > > This is for kidney or muscle bx frozens for immmunofluroescence or immunoperoxidase staining. > > Normally they air dry for at least 15 minutes (just waiting for frozen sectioning to be completed) before going into acetone. Just wondering if we can reduce complexity... > > I haven't seen anything saying why cold acetone is used, just instructions to do so. I always wonder about such things... > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology UC San Francisco Medical Center > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From Timothy.Morken at ucsf.edu Fri Oct 26 10:09:02 2018 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 26 Oct 2018 15:09:02 +0000 Subject: [Histonet] Frozen sections and cold acetone... In-Reply-To: <23e7514abddf4a31a2a060366a3c7c3d@SVDCMBX-MEX024.nswhealth.net> References: <7b25693f-e20f-67ca-fad7-dee05adee791@shaw.ca> <23e7514abddf4a31a2a060366a3c7c3d@SVDCMBX-MEX024.nswhealth.net> Message-ID: Tony, thanks for this. Our continued use of IF rather than IPOX is due to seemingly better signal/noise with IF. And now we have developed "rescue" techniques for IF on FFPE sections for cases in which the frozen portion is inadequate. Since we started that we have had more requests for IF of immunoglobulins on other FFPE tissues (liver, nerve, others) due to better signal/noise with IF vs IPOX of the same antibodies. On the question of cold vs room temperature acetone, I remember WAY back when we would cut frozens and immediately put them in -20C acetone for fixation. We got away from that and now put them in 2C acetone after they have dried for 10-30 minutes. Results are the same (at least for Ig and complement antigen staining). So, I'm going to try room temp acetone to see the results. Also some have mentioned they don't fix in acetone at all with good results. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Tony Henwood (SCHN) [mailto:tony.henwood at health.nsw.gov.au] Sent: Thursday, October 25, 2018 4:52 PM To: Bryan Llewellyn; Morken, Timothy Cc: histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] Frozen sections and cold acetone... Hi Tim (and Bryan), This is from a book chapter I wrote (though not published as yet), that might be useful: "It is important that fixation has minimal effect on antigenic determinates, allowing recognition by appropriate antibodies. Fixatives can be physical or chemical in action. Physical fixatives, for example heating sections or cells on to slides, alcohol or acetone treatment, preserve tissues by dehydrating cells. Alcohol and acetone possibly also dissolve lipids from cell membranes facilitating antibody access." And "With today's modern immunohistochemical methods being done quite successfully on formalin-fixed, paraffin-embedded sections, it is mystifying as to why immunofluorescence is still preferred for immunopathological renal and skin diagnosis. Immunofluorescence remains the most popular method of detecting immunoglobulins and complement deposits, with 83% of British laboratories using this technique (36). Technically, frozen sections are cut, dried, rinsed in buffer, optimally diluted FITC labelled antibodies are added to sections, incubated for 30-60 minutes, non-bound antibody is rinsed from the sections with buffer, the sections are coverslipped with a non-fluorescing aqueous mountant and finally viewed using a microscope equipped with an ultra-violet light source; overall quite a simple technique." "The detection of tissue-bound autoantibodies is rendered difficult by the presence of abundant plasma proteins, which must be removed to avoid non-specific staining. This has resulted in a continuing popularity of frozen sections and immunofluorescence techniques in immunopathology, where they have been superseded in most other branches of immunohistochemistry by methods that are applicable to paraffin wax sections. In paraffin wax sections, the plasma proteins have been fixed in the tissues and must be removed by a digestion process rather than simple washing (37). Unfortunately, too much digestion may destroy tissue integrity and possibly remove the immune complexes, resulting in a false negative result. Furthermore, digestion conditions are difficult to standardize. The times required vary with the degree of fixation and section thickness (38). Frozen sections of non-fixed tissue, when used for the detection of bound-immune complexes, give clean localization with minimal background without the requirement for enzymatic pre-digestion. The plasma immunoglobulins, being loosely bound, if bound at all, to the surrounding tissue elements are easily dissolved in the warm buffer pre-incubation step. Bound immune-complexes seem to be unaffected." 36. Furness PN & Boyd S (1996). Electron microscopy and immunocytochemistry in the assessment of renal biopsy specimens: actual and optimal practice. Journal of clinical pathology, 49(3):233-237 37. Furness PN (2000). ACP Best practice No 160. Renal biopsy specimens. Journal of clinical pathology, 53(6):433-438. 38. Lehman JS & Camilleri MJ (2013). Potential diagnostic pitfall in direct immunofluorescence evaluation for vasculitis in skin biopsies: fluorescent secretory granules within eccrine glands. Journal of cutaneous pathology 40:603-605 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Bryan Llewellyn via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 26 October 2018 5:54 AM To: Histonet Subject: Re: [Histonet] Frozen sections and cold acetone... Cold acetone, and cold ethanol, were used to fix tissues because they left enzymes unaffected and still demonstrable. This was in the early days of enzyme histochemistry. Pearse' Histochemistry: Theoretical and applied,3rd edition, volume 1, page 85 discusses it. I could send a scan if you wanted. Bryan Llewellyn Morken, Timothy via Histonet wrote: > Can anyone give me a rational for using cold (refrig or freezer-temp) acetone to fix frozen sections? Or a rational for using RT acetone. > > This is for kidney or muscle bx frozens for immmunofluroescence or immunoperoxidase staining. > > Normally they air dry for at least 15 minutes (just waiting for frozen sectioning to be completed) before going into acetone. Just wondering if we can reduce complexity... > > I haven't seen anything saying why cold acetone is used, just instructions to do so. I always wonder about such things... > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology UC San Francisco Medical Center > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From TWELLEN at LHS.ORG Fri Oct 26 12:14:17 2018 From: TWELLEN at LHS.ORG (Wellen, Terry :LLS Lab) Date: Fri, 26 Oct 2018 17:14:17 +0000 Subject: [Histonet] Histotechs abroad? Message-ID: Does anyone know if it is possible to work overseas? (esp. Spain) What is the drill? Terrence D. Wellen HT (ASCP) Technical Specialist LCL Pathology/IHC Portland, OR 503-944-7923 From carl.hobbs at kcl.ac.uk Fri Oct 26 13:43:44 2018 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 26 Oct 2018 18:43:44 +0000 Subject: [Histonet] Frozen sections and cold acetone Message-ID: I have always "fixed" in RT acetone for 10 mins Have compared 0-60 mins/4C to RT acetone Lower temps just limit the rate of reaction, imho. I note "nuclear streaming" when I use acetone at any temp/time. Imho, acetone is not an effective fixative....it's a delipidiser. So, give it 10 mins at RT. If anyone thinks it's effective because it is a dehydrant?.well, one re- hydrates the section to carry out IHC/ICC/IF. It works very well for only a few abs. When testing new abs out on FS/cell monolayers ( unfixed) I always compare Formalin, Acetone, Methanol and methanol/acetone 1:1 Imho Best wishes to a great site/membership. Carl From VKurth at uwhealth.org Fri Oct 26 15:07:22 2018 From: VKurth at uwhealth.org (Kurth Virginia L.) Date: Fri, 26 Oct 2018 20:07:22 +0000 Subject: [Histonet] REPEATS IN IHC Message-ID: Hello, I was wondering if anyone in the histology laboratory (IHC) has a limit on how many times one would repeat a stain; for example but not Limited too: The tissue fell off, negative control, wrong block,etc.. for IHC DAB and/or ISH. Thanks Ginny From rjbuesa at yahoo.com Sat Oct 27 08:48:06 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Sat, 27 Oct 2018 13:48:06 +0000 (UTC) Subject: [Histonet] REPEATS IN IHC In-Reply-To: References: Message-ID: <1773692175.85882.1540648086619@mail.yahoo.com> Ginny:It seems you are asking for a "problem" average and that will vary per laboratory and how the whole process is carried out.Section washed out from the slide usually is consequence of poor processing or sectioning and has nothing to do with IHC, although, perhaps, a wrongly performed HIER can cause it.Wrong block is a mistake also having nothing to do with IHC and is always caused by an inattentive HT not paying attention to the process.My point is that, essentially, the lab should try to get to "0" mistakes and any one has to be documented in the QC record but it will be interesting to find out the "mistakes rate" you find and I think you should share it with all.Ren? On Friday, October 26, 2018 4:41 PM, Kurth Virginia L. via Histonet wrote: Hello, I was wondering if anyone in the histology laboratory (IHC) has a limit on how many times one would repeat a stain; for example but not Limited too:? The tissue fell off, negative control, wrong block,etc.. for IHC DAB and/or ISH.? Thanks Ginny _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet | | Virus-free. www.avast.com | From tbraud at holyredeemer.com Mon Oct 29 08:55:43 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 29 Oct 2018 13:55:43 +0000 Subject: [Histonet] Repeats in IHC Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF83B84@HRHEX02-HOS.holyredeemer.local> Our repeat limits differ. If it is due to tissue falling off, we will try at least 3 times If it is due to unexpected, but within control, we will repeat it 2 times Then if it is still problematic for the above 2, we will send out the block for staining. If it is due to technical error (wrong block, wrong stain) we will perform the stain until correct, and it better not be more than once, LOL Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Histotechs abroad? (Wellen, Terry :LLS Lab) 2. Re: Frozen sections and cold acetone (Hobbs, Carl) 3. REPEATS IN IHC (Kurth Virginia L.) 4. Re: REPEATS IN IHC (Rene J Buesa) Message: 3 Date: Fri, 26 Oct 2018 20:07:22 +0000 From: Kurth Virginia L. Subject: [Histonet] REPEATS IN IHC Hello, I was wondering if anyone in the histology laboratory (IHC) has a limit on how many times one would repeat a stain; for example but not Limited too: The tissue fell off, negative control, wrong block,etc.. for IHC DAB and/or ISH. Thanks Ginny ------------------------------ Message: 4 Date: Sat, 27 Oct 2018 13:48:06 +0000 (UTC) From: Rene J Buesa To: "Kurth Virginia L." , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] REPEATS IN IHC Message-ID: <1773692175.85882.1540648086619 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Ginny:It seems you are asking for a "problem" average and that will vary per laboratory and how the whole process is carried out.Section washed out from the slide usually is consequence of poor processing or sectioning and has nothing to do with IHC, although, perhaps, a wrongly performed HIER can cause it.Wrong block is a mistake also having nothing to do with IHC and is always caused by an inattentive HT not paying attention to the process.My point is that, essentially, the lab should try to get to "0" mistakes and any one has to be documented in the QC record but it will be interesting to find out the "mistakes rate" you find and I think you should share it with all.Ren? On Friday, October 26, 2018 4:41 PM, Kurth Virginia L. via Histonet wrote: Hello, I was wondering if anyone in the histology laboratory (IHC) has a limit on how many times one would repeat a stain; for example but not Limited too:? The tissue fell off, negative control, wrong block,etc.. for IHC DAB and/or ISH.? Thanks Ginny _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lkc0001 at auburn.edu Mon Oct 29 11:06:42 2018 From: lkc0001 at auburn.edu (Lisa Parsons) Date: Mon, 29 Oct 2018 16:06:42 +0000 Subject: [Histonet] LYVE1 and CD31 in dogs Message-ID: I am having issues getting LYVE1 and CD31 to work in dogs. In the past, I have used JC/70A clone( CD31) with success. This is my first try with LYVE1. Does anyone have a protocol that works in dogs they would be willing to share? Thank you! From cforster at umn.edu Mon Oct 29 11:13:11 2018 From: cforster at umn.edu (Colleen Forster) Date: Mon, 29 Oct 2018 11:13:11 -0500 Subject: [Histonet] LYVE1 and CD31 in dogs In-Reply-To: References: Message-ID: Im am also interested. Having the same issue. Colleen Forster On Mon, Oct 29, 2018 at 11:06 AM, Lisa Parsons via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I am having issues getting LYVE1 and CD31 to work in dogs. In the past, I > have used JC/70A clone( CD31) with success. This is my first try with > LYVE1. Does anyone have a protocol that works in dogs they would be willing > to share? Thank you! > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at holml at umn.edu * From jcomensk at yahoo.com Mon Oct 29 12:50:19 2018 From: jcomensk at yahoo.com (John Comensky) Date: Mon, 29 Oct 2018 13:50:19 -0400 Subject: [Histonet] Microscope service Message-ID: <87C909FE-7821-468B-BD3A-ABF77F6D361F@yahoo.com> Histonet- Anybody in the Albany NY area know of a company/individual who can service microscopes for a school science program? Not free work, just willing to work. Feel free to contact me- jcomensk at yahoo.com Sent from my iPhone From katherine at ka-recruiting.com Mon Oct 29 14:44:05 2018 From: katherine at ka-recruiting.com (Katherine Marano) Date: Mon, 29 Oct 2018 15:44:05 -0400 Subject: [Histonet] Histotech needed in California Message-ID: Hi Histonetters. Happy Monday! I just got in an opening at a top hospital in southern California. My client is looking to hire a full-time and permanent Histology Technician. It is a M-F 5 am-7 am start (not sure exactly when right now), 8 hr shift. They are looking for a candidate with routine and special stain experience, preferably in a hospital setting. Minimum 3 years? experience. If you are interested could you send me a resume and a good time to give you a quick phone call? Thank you! Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From relia1 at earthlink.net Tue Oct 30 11:17:51 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 30 Oct 2018 12:17:51 -0400 Subject: [Histonet] Happy Halloween !! No tricks here and a special treat at the end of this message!!! Message-ID: <008f01d4706c$1b43e400$51cbac00$@earthlink.net> Hi Histonetters, Happy Halloween!!! TRICK or TREAT!!!!! CHECK OUT A REALLY COOL IDEA FOR YOUR HALLOWEEN CELEBRATION AT THE END OF THIS EMAIL! The TRICK is finding the right job opportunity for you. The TREAT is with my help it can be relatively painless. ? I will help you with your resume, ? Coach you through the interview and offer process ? And refer you to positions based on the criteria you give me. All of the positions I work with are fulltime permanent positions with some of the best facilities Nationwide. My clients offer excellent compensation including competitive salaries, great benefits, relocation assistance/sign on bonuses. YOU CAN START RIGHT AWAY OR AFTER THE HOLIDAYS!! Histonetters, I have exciting opportunities in these areas! Management/Supervisor/Lead Tech: Histology Lab Manager Fayetteville, NC Histology Product Sales Mgr. Dallas, TX Histology Product Sales Mgr. Midwest Histotechnicians/Histotechnologists: Histotech Springfield, MA ?days @ great lab!! Senior Tech Norfolk, VA ? days and sign on bonus! Histotechnician Fayetteville, NC ? days and relo!! Histo Sales Dallas, TX ? Get out of the Lab! Histotech Norfolk, VA ? sign on bonus! Histology Tech San Diego, CA ? days!! Histotech Fayetteville, AR ? Days!! If you or any of your friends would like more information on any of these positions or if you would like to discuss opportunities in other areas or future job searches please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or on my cell/text at 407-353-5070 or at relia1 at earthlink.net Remember I offer over 20 years of recruiting and employment counseling experience, knowledgeable, confidential and responsive service to you and your friends and a permanent placement practice dedicated to the histology profession.? I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember It never hurts to keep an eye open, even if you are happy in your present job. HOW DO YOU LIKE THIS IDEA FOR HALLOWEEN FUN? Four Ingredient Candy Corn Punch For one serving: 1/3 cup frozen crushed pineapple 1/3 cup orange juice Whipped cream Candy corn for garnish. Combine 1st 2 ingredients, top with whipped cream and candy corn. (Spiking with a 5th ingredient for the adults is optional) Happy Halloween!!!!!!!!!!! p.s. How are you celebrating Halloween? Pam ? 866-607-3542 (866-60RELIA) Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From sandra.cheasty at wisc.edu Tue Oct 30 11:37:33 2018 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Tue, 30 Oct 2018 16:37:33 +0000 Subject: [Histonet] Sakura Tape Coverslipper Advice Needed! Message-ID: Hello all, Our Sakura tape coverslipper is doing something I've never encountered before - the lever that holds the blade and goes up and down and cuts the tape, has gotten sticky and slow. It will chop down fine, but has a hard time returning to the up position. Before I take the back off, I'm just wondering if this has happened to anyone else, and if so, how it was rectified. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From tissuearray at hotmail.com Tue Oct 30 14:33:58 2018 From: tissuearray at hotmail.com (Thom) Date: Tue, 30 Oct 2018 19:33:58 +0000 Subject: [Histonet] New Research Histologist Position in Salt Lake City, Utah USA Message-ID: Hi Histologists, There is a new job posting for a Histologist in Salt Lake City, Utah. This is for a research histologist position to work in the Intermountain Biorepository for Intermountain Healthcare. The Intermountain Biorepository has only been open for about a month. They have one histologist and looking to hire another. Here is a link to the job posting. Job ID: 222729 https://jobs.intermountainhealthcare.org/res_viewjob.html?erjob=204672:en_US&tmReferrerUserId=88DC0764FCE942D58D97895B6D37E28F From relia1 at earthlink.net Wed Oct 31 10:02:33 2018 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 31 Oct 2018 11:02:33 -0400 Subject: [Histonet] Happy Halloween !! No tricks here and a special treat at the end of this message!!! Message-ID: <007f01d4712a$c093bde0$41bb39a0$@earthlink.net> Hi Histonetters, Happy Halloween!!! TRICK or TREAT!!!!! CHECK OUT A REALLY COOL IDEA FOR YOUR HALLOWEEN CELEBRATION AT THE END OF THIS EMAIL! The TRICK is finding the right job opportunity for you. The TREAT is with my help it can be relatively painless. ? I will help you with your resume, ? Coach you through the interview and offer process ? And refer you to positions based on the criteria you give me. All of the positions I work with are fulltime permanent positions with some of the best facilities Nationwide. My clients offer excellent compensation including competitive salaries, great benefits, relocation assistance/sign on bonuses. YOU CAN START RIGHT AWAY OR AFTER THE HOLIDAYS!! Histonetters, I have exciting opportunities in these areas! Management/Supervisor/Lead Tech: Histology Lab Manager Fayetteville, NC Histology Product Sales Mgr. Dallas, TX Histology Product Sales Mgr. Middle U.S. Histotechnicians/Histotechnologists: Histotech Springfield, MA ?days @ great lab!! Senior Tech Norfolk, VA ? days and sign on bonus! Histotechnician Fayetteville, NC ? days and relo!! Histo Sales Dallas, TX ? Get out of the Lab! Histo Sales Middle U.S. ? Get out of the lab! Histotech Norfolk, VA ? sign on bonus! Histology Tech San Diego, CA ? days!! Histotech Fayetteville, AR ? Days!! If you or any of your friends would like more information on any of these positions or if you would like to discuss opportunities in other areas or future job searches please contact me. I would be more than happy to assist you. You can reach me at 866-607-3542 or on my cell/text at 407-353-5070 or at relia1 at earthlink.net Remember I offer over 20 years of recruiting and employment counseling experience, knowledgeable, confidential and responsive service to you and your friends and a permanent placement practice dedicated to the histology profession.? I work with facilities nationwide and I will keep your resume confidential. I will only represent you to jobs you tell me you are interested in looking into. Remember It never hurts to keep an eye open, even if you are happy in your present job. HOW DO YOU LIKE THIS IDEA FOR HALLOWEEN FUN? Four Ingredient Candy Corn Punch For one serving: 1/3 cup frozen crushed pineapple 1/3 cup orange juice Whipped cream Candy corn for garnish. Combine 1st 2 ingredients, top with whipped cream and candy corn. (Spiking with a 5th ingredient for the adults is optional) Happy Halloween!!!!!!!!!!! p.s. How are you celebrating Halloween? Pam ? 866-607-3542 (866-60RELIA) Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From allanvv at gmail.com Wed Oct 31 16:46:58 2018 From: allanvv at gmail.com (Allan Wang) Date: Wed, 31 Oct 2018 17:46:58 -0400 Subject: [Histonet] Clear-Rite 3 dirty slides Message-ID: Hi histonet, For coverslipping Ventana IHC slides I have dish soap, rinse, alcohol 80%, 100%, 100%, 100%, followed by three Clear-Rite 3 instead of xylene. In the past I didn't have this issue, but now I frequently get a lot of residue on the slides after they enter the Clear-Rite. If I completely dump all 3 containers of Clear-Rite 3 it gets rid of the problem but I'd have to do this every month which is wasteful. If I just dump the first container and move everything up, the problem still remains. Images: https://i.imgur.com/GTQJe2W.jpg https://i.imgur.com/np8B5P2.jpg https://i.imgur.com/SLypUAy.jpg Anyone know what this contamination is and the best way to reduce it? Allan From jaylundgren at gmail.com Wed Oct 31 19:54:36 2018 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 31 Oct 2018 20:54:36 -0400 Subject: [Histonet] Histotechs abroad? In-Reply-To: References: Message-ID: I understand there is a shortage of histotechs in Syria, also eastern Ukraine. Have fun! On Fri, Oct 26, 2018 at 1:25 PM Wellen, Terry :LLS Lab via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Does anyone know if it is possible to work overseas? (esp. Spain) > What is the drill? > > Terrence D. Wellen HT (ASCP) > Technical Specialist > LCL Pathology/IHC > Portland, OR > 503-944-7923 > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >