From plucas at biopath.org Thu Nov 1 10:01:32 2018 From: plucas at biopath.org (Paula) Date: Thu, 1 Nov 2018 08:01:32 -0700 Subject: [Histonet] Instrument Correlation Message-ID: <003201d471f3$c7bd71a0$573854e0$@biopath.org> Hello everyone, Could you recommend/suggest how the correlation between 2 IHC Bond instruments is performed and written out for them? This has never been brought up before and I'm just starting my research. Thank you in advance, Paula From Lisa.White3 at va.gov Thu Nov 1 12:20:37 2018 From: Lisa.White3 at va.gov (White, Lisa M.) Date: Thu, 1 Nov 2018 17:20:37 +0000 Subject: [Histonet] Pinning Gross Specimens Message-ID: Hello all, Does anyone know of a container with lid that has cork in the bottom? This would be utilized to pin out gross specimens for fixation. If so can you send me vendor info? Lisa White, HT(ASCP) James H. Quillen VAMC From llewllew at shaw.ca Thu Nov 1 12:41:23 2018 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Thu, 1 Nov 2018 10:41:23 -0700 Subject: [Histonet] Pinning Gross Specimens In-Reply-To: References: Message-ID: If you can't find suitable pin out boards, they can be made easily enough. Obtain some suitably sized plastic containers with lids and fill them an inch deep with used, filtered, paraffin wax that would otherwise be discarded. After using, just remelt, filter if needed, then harden again. Bryan Llewellyn White, Lisa M. via Histonet wrote: > Hello all, > > Does anyone know of a container with lid that has cork in the bottom? This would be utilized to pin out gross specimens for fixation. If so can you send me vendor info? > > Lisa White, HT(ASCP) > James H. Quillen VAMC > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tbraud at holyredeemer.com Thu Nov 1 12:43:53 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 1 Nov 2018 17:43:53 +0000 Subject: [Histonet] Instrument to instrument correlation Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF84AC1@HRHEX02-HOS.holyredeemer.local> Hi Paula - I use duplicate microarrays (TMAs), each with at least 10 positive cases and 10 negative cases, one TMA slide on each instrument for each antibody. Passed CAP with no problems. Hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 3. Instrument Correlation (Paula) Date: Thu, 1 Nov 2018 08:01:32 -0700 From: "Paula" Hello everyone, Could you recommend/suggest how the correlation between 2 IHC Bond instruments is performed and written out for them? This has never been brought up before and I'm just starting my research. Thank you in advance, Paula From allanvv at gmail.com Thu Nov 1 12:45:06 2018 From: allanvv at gmail.com (Allan Wang) Date: Thu, 1 Nov 2018 13:45:06 -0400 Subject: [Histonet] Clear-Rite 3 dirty slides In-Reply-To: References: Message-ID: Thanks all, looks like the consensus is that water into the clearing agent causes it and I should change out alcohol more frequently. Allan On Wed, Oct 31, 2018 at 5:46 PM Allan Wang wrote: > Hi histonet, > > For coverslipping Ventana IHC slides I have dish soap, rinse, alcohol 80%, > 100%, 100%, 100%, followed by three Clear-Rite 3 instead of xylene. In the > past I didn't have this issue, but now I frequently get a lot of residue on > the slides after they enter the Clear-Rite. > > If I completely dump all 3 containers of Clear-Rite 3 it gets rid of the > problem but I'd have to do this every month which is wasteful. If I just > dump the first container and move everything up, the problem still remains. > > Images: > https://i.imgur.com/GTQJe2W.jpg > https://i.imgur.com/np8B5P2.jpg > https://i.imgur.com/SLypUAy.jpg > > Anyone know what this contamination is and the best way to reduce it? > > Allan > From Douglas.Porter at sparrow.org Thu Nov 1 12:47:38 2018 From: Douglas.Porter at sparrow.org (Porter, Douglas) Date: Thu, 1 Nov 2018 17:47:38 +0000 Subject: [Histonet] [EXTERNAL] Re: Pinning Gross Specimens In-Reply-To: References: Message-ID: <330319ed6405457d97a022f7937d2d47@EXCHMBPVAS01.shs.org> Bryan's advice is spot on. We do the same thing and cut the paraffin into sizes we need. With some specimens, you can pin them down and return them to the containers they arrived in. Provided they arrive in containers appropriate for the size of the specimen...wink, wink!! Douglas A. Porter, HT (ASCP) Pathologist Assistant Anatomic Pathology IT Coordinator Sparrow Center for Laboratory Medicine Department of Pathology 3392 Patient Care Drive Lansing, MI 48911 517-371-9481 (phone) 517-371-9540 (fax) douglas.porter at sparrow.org -----Original Message----- From: Bryan Llewellyn via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, November 01, 2018 1:41 PM To: White, Lisa M.; histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Pinning Gross Specimens Warning: This email originated from outside of Sparrow. Do not click links or open attachments unless you recognize the sender and are expecting the message. ---------------------------------------------------------------------- If you can't find suitable pin out boards, they can be made easily enough. Obtain some suitably sized plastic containers with lids and fill them an inch deep with used, filtered, paraffin wax that would otherwise be discarded. After using, just remelt, filter if needed, then harden again. Bryan Llewellyn White, Lisa M. via Histonet wrote: > Hello all, > > Does anyone know of a container with lid that has cork in the bottom? This would be utilized to pin out gross specimens for fixation. If so can you send me vendor info? > > Lisa White, HT(ASCP) > James H. Quillen VAMC > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=qgJBLQvENW4Kb9JcrSOXvj11QOUUKGR5N2IUAtns1Jg&r=6pgpgKsLHvt-FitLISss8MQQkPawKdpRw8msCll96Ts&m=8CDk8DLT4_gRkMQLtEWlhjd-HEI1BOr6w2rsJqQ4GiI&s=6eRg8jZIzOA_oYi3cofPPJr2lgpIG3ALmsL8WV8X46k&e= > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=qgJBLQvENW4Kb9JcrSOXvj11QOUUKGR5N2IUAtns1Jg&r=6pgpgKsLHvt-FitLISss8MQQkPawKdpRw8msCll96Ts&m=8CDk8DLT4_gRkMQLtEWlhjd-HEI1BOr6w2rsJqQ4GiI&s=6eRg8jZIzOA_oYi3cofPPJr2lgpIG3ALmsL8WV8X46k&e= ________________________________ CONFIDENTIALITY NOTICE: This email communication may contain private, confidential, or legally privileged information intended for the sole use of the designated and/or duly authorized recipient(s). If you are not the intended recipient or have received this email in error, please notify the sender immediately by email and permanently delete all copies of this email including all attachments without reading them. If you are the intended recipient, secure the contents in a manner that conforms to all applicable state and/or federal requirements related to privacy and confidentiality of such information. ________________________________ From KBummer at nektar.com Thu Nov 1 13:20:41 2018 From: KBummer at nektar.com (Bummer, Katherine) Date: Thu, 1 Nov 2018 18:20:41 +0000 Subject: [Histonet] [EXTERNAL] Histonet Digest, Vol 180, Issue 1 In-Reply-To: References: Message-ID: Hello Allan, I have been seeing the same thing but with HistoClearII. I have been using alkaline phosphatase substrates on some double chromogenic staining that requires me to either dehydrate and clear with a non-Xylene solvent or just mount with aqueous PVP mounting media. I had thought that it was perhaps some H2O carrying over into my dehydration and clearing but never did resolve fully. Any info that comes of this I would like to be included on as well. Thank you. Kate -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Thursday, November 1, 2018 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 180, Issue 1 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From Jcox90 at yahoo.com Thu Nov 1 15:40:39 2018 From: Jcox90 at yahoo.com (Jill Cox) Date: Thu, 1 Nov 2018 13:40:39 -0700 Subject: [Histonet] Sub X in lieu of Clear rite 3 Message-ID: <17CAB909-EBA0-48F9-A0F4-C458BADEA8BE@yahoo.com> Hi all, We currently use clear rite 3 in our processor and stain-line and have had no problems for many years. It is getting so expensive now so we are considering switching to Sub X. Does anybody have any feedback on this? Thank you in advance! Sent from my iPhone From lmarie08 at uga.edu Fri Nov 2 08:11:35 2018 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Fri, 2 Nov 2018 13:11:35 +0000 Subject: [Histonet] microtome reviews Message-ID: Hi Everyone! We are looking into getting an embedding center and I was wondering if I could get some reviews from you about them. Specifically we are looking at the Tanner Scientific TN1600, the Avantik EM8, and the Leica Arcadia. Any thoughts on any of these would be much appreciated! Have a wonderful day, Lauren From robrankin at rankinbiomed.com Fri Nov 2 13:10:09 2018 From: robrankin at rankinbiomed.com (Rob Rankin) Date: Fri, 2 Nov 2018 14:10:09 -0400 Subject: [Histonet] Need a Used Leica Peloris II Message-ID: In need of a Peloris II (Rob Rankin) Hello all Histonetters, If any labs have a Leica Peloris II that they would like to sell, please let me know. Rankin Biomedical is in the market to buy one. Respectfully, *Rob Rankin, MSM, SM(ASCP)* Founder & CEO 248.625.4104 x 0012 *RANKIN* 14515 Mackey Road Holly, MI 48442 www.rankinbiomed.com facebook.com/rankinbiomed twitter.com/rankinbiomed.com From CarenAnn.Echague at DignityHealth.org Mon Nov 5 09:06:21 2018 From: CarenAnn.Echague at DignityHealth.org (Echague, Caren Ann - MGH) Date: Mon, 5 Nov 2018 15:06:21 +0000 Subject: [Histonet] Instrument to instrument correlation In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8ECF84AC1@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8ECF84AC1@HRHEX02-HOS.holyredeemer.local> Message-ID: <01edfc6bb4314ddcb43dabd39f68f1dc@PHX-EXCH-014.chw.edu> What was the vendor for your TMAs? I have been shopping around and trying to find the best ones. =) Cae -----Original Message----- From: Terri Braud [mailto:tbraud at holyredeemer.com] Sent: Thursday, November 01, 2018 10:44 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Instrument to instrument correlation Hi Paula - I use duplicate microarrays (TMAs), each with at least 10 positive cases and 10 negative cases, one TMA slide on each instrument for each antibody. Passed CAP with no problems. Hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 3. Instrument Correlation (Paula) Date: Thu, 1 Nov 2018 08:01:32 -0700 From: "Paula" Hello everyone, Could you recommend/suggest how the correlation between 2 IHC Bond instruments is performed and written out for them? This has never been brought up before and I'm just starting my research. Thank you in advance, Paula From relia1 at earthlink.net Mon Nov 5 10:18:41 2018 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 5 Nov 2018 11:18:41 -0500 Subject: [Histonet] What would your advice be for a New Grad? I speak to a class on Thursday! Message-ID: <01d501d47523$37a5b9a0$a6f12ce0$@earthlink.net> Dear Histonetters, I hope this is the start to a fantastic week! I want to take this opportunity to thank everyone who responded for their fantastic ideas for promoting the histology field. Thursday morning I will be speaking to a group of histotechs getting ready to head out to their externships and then into their first jobs in the field. My question to you is if you had one piece of advice for them what would it be? Rather than send another email later this week let me take a minute and tell you about the opportunities I am working on that I am most excited about. I am excited about these opportunities because all of these positions are full time, permanent and offer excellent compensation, benefits and relocation assistance. Most are DAY SHIFT!!! They are ready to interview and hire NOW and you can start now or AFTER the Holidays!! Without Further Ado here are the exciting opportunities I am currently recruiting for: Histology Leadership Opportunities: Fayetteville, NC Histology Territory Sales Manager Texas OH Histotechnician/Histotechnologist ? DAYS Austin, TX Springfield, MA Fayetteville, NC Norfolk, VA Histonetters, if you or anyone you know are interested in hearing more about any of these opportunities or have another type of position or area in mind and would like some help in your job search please let me know. Just shoot me an e-mail at relia1 at earthlink.net or give me a call toll free at 866-607-3542 or on my cell at 407-353-5070. And if you have a second I would love to hear what you would like the new grads to know. Thanks again!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From boznpl at aol.com Mon Nov 5 10:44:23 2018 From: boznpl at aol.com (Laurie Redmond) Date: Mon, 5 Nov 2018 16:44:23 +0000 (UTC) Subject: [Histonet] PAS Stain References: <1715295197.1084926.1541436263630.ref@mail.yahoo.com> Message-ID: <1715295197.1084926.1541436263630@mail.yahoo.com> Does anyone else out there use warm water to rinse the PAS slides after the Schiff's reagent?? Can anyone comment on whether using warm water vs. cold water would have any affect on the stain? Laurie Redmond HT (ASCP) From TNMayer at mdanderson.org Mon Nov 5 12:24:36 2018 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Mon, 5 Nov 2018 18:24:36 +0000 Subject: [Histonet] What would your advice be for a New Grad? I speak Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC883ACF9AD2@D1PWPEXMBX06.mdanderson.edu> Pam, My advice would be to remember that they should first master their entry-level competencies before focusing on advanced techniques. The ability to master those techniques prove invaluable when taking the BOC and maintaining employment. Toysha N. Mayer D.H.Sc., MBA, HT(ASCP) Assistant Professor/Education Coordinator HTL Program MD Anderson School of Health Professions 713.563.3481 tnmayer at mdanderson.org Message: 2 Date: Mon, 5 Nov 2018 11:18:41 -0500 From: "Pam Barker" To: "Histonetters Histonet" Subject: [Histonet] What would your advice be for a New Grad? I speak to a class on Thursday! Message-ID: <01d501d47523$37a5b9a0$a6f12ce0$@earthlink.net> Content-Type: text/plain; charset="iso-8859-1" Dear Histonetters, I hope this is the start to a fantastic week! I want to take this opportunity to thank everyone who responded for their fantastic ideas for promoting the histology field. Thursday morning I will be speaking to a group of histotechs getting ready to head out to their externships and then into their first jobs in the field. My question to you is if you had one piece of advice for them what would it be? Rather than send another email later this week let me take a minute and tell you about the opportunities I am working on that I am most excited about. I am excited about these opportunities because all of these positions are full time, permanent and offer excellent compensation, benefits and relocation assistance. Most are DAY SHIFT!!! They are ready to interview and hire NOW and you can start now or AFTER the Holidays!! Without Further Ado here are the exciting opportunities I am currently recruiting for: Histology Leadership Opportunities: Fayetteville, NC Histology Territory Sales Manager Texas OH Histotechnician/Histotechnologist ? DAYS Austin, TX Springfield, MA Fayetteville, NC Norfolk, VA Histonetters, if you or anyone you know are interested in hearing more about any of these opportunities or have another type of position or area in mind and would like some help in your job search please let me know. Just shoot me an e-mail at relia1 at earthlink.net or give me a call toll free at 866-607-3542 or on my cell at 407-353-5070. And if you have a second I would love to hear what you would like the new grads to know. Thanks again!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From lmarie08 at uga.edu Mon Nov 5 12:26:22 2018 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Mon, 5 Nov 2018 18:26:22 +0000 Subject: [Histonet] job opportunity Message-ID: Hello Histonetters! A state- run Histology lab is looking to hire a lab manager. This lab is at the University of Georgia in Athens, GA. It is M-F 8 a.m.- 5 pm. shift. Great benefits, including University- wide week off at Christmas- paid! Preferably HT or HTL- certified, IHC experience, and experience as a lab manager. Not having these is not necessarily a deal-breaker! Email me at: lmarie08 at uga.edu for more information and/or to send your resume. Happy day, Lauren From TNMayer at mdanderson.org Mon Nov 5 12:31:20 2018 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Mon, 5 Nov 2018 18:31:20 +0000 Subject: [Histonet] PAS Stain Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC883ACF9B0D@D1PWPEXMBX06.mdanderson.edu> Laurie, The warm water helps the color of the Schiff develop in a more vibrant pattern. If you allow the slides to sit in warm water for 2 min, then rinse you also do not have to use the metabisulfite rinses to remove excess stain. Toysha N. Mayer D.H.Sc., MBA, HT(ASCP) Assistant Professor/Education Coordinator HTL Program MD Anderson School of Health Professions 713.563.3481 tnmayer at mdanderson.org ------------------------------ Message: 3 Date: Mon, 5 Nov 2018 16:44:23 +0000 (UTC) From: Laurie Redmond To: histonet at lists.utsouthwestern.edu Subject: [Histonet] PAS Stain Message-ID: <1715295197.1084926.1541436263630 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Does anyone else out there use warm water to rinse the PAS slides after the Schiff's reagent?? Can anyone comment on whether using warm water vs. cold water would have any affect on the stain? Laurie Redmond HT (ASCP) ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 180, Issue 4 **************************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From erin.mccarthy at tempus.com Mon Nov 5 13:12:10 2018 From: erin.mccarthy at tempus.com (Erin McCarthy) Date: Mon, 5 Nov 2018 13:12:10 -0600 Subject: [Histonet] What would your advice be for a New Grad? I speak In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC883ACF9AD2@D1PWPEXMBX06.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC883ACF9AD2@D1PWPEXMBX06.mdanderson.edu> Message-ID: I agree with what Toysha said, and also don't take your more experienced techs for granted! There is great value in techs that have been at this for a long time. They may not seem tech savvy, or fast to you now, but some of them have been at this longer than us younger techs have been alive! Some of the greatest cutting troubleshooting tips I have came as the result of the knowledge of these veterans! You truly do not realize how helpful they are/have been until they are no longer your neighbor to ask questions of! On Mon, Nov 5, 2018 at 1:01 PM Mayer,Toysha N via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Pam, > > My advice would be to remember that they should first master their > entry-level competencies before focusing on advanced techniques. The > ability to master those techniques prove invaluable when taking the BOC and > maintaining employment. > > > Toysha N. Mayer D.H.Sc., MBA, HT(ASCP) > Assistant Professor/Education Coordinator > HTL Program > MD Anderson School of Health Professions > 713.563.3481 > tnmayer at mdanderson.org > > > > > > > > Message: 2 > Date: Mon, 5 Nov 2018 11:18:41 -0500 > From: "Pam Barker" > To: "Histonetters Histonet" > Subject: [Histonet] What would your advice be for a New Grad? I speak > to a class on Thursday! > Message-ID: <01d501d47523$37a5b9a0$a6f12ce0$@earthlink.net> > Content-Type: text/plain; charset="iso-8859-1" > > Dear Histonetters, > I hope this is the start to a fantastic week! > > I want to take this opportunity to thank everyone who responded for their > fantastic ideas for promoting the histology field. > > Thursday morning I will be speaking to a group of histotechs getting ready > to head out to their externships and then into their first jobs in the > field. > > My question to you is if you had one piece of advice for them what would > it be? > > Rather than send another email later this week let me take a minute and > tell you about the opportunities I am working on that I am most excited > about. > I am excited about these opportunities because all of these positions are > full time, permanent and offer excellent compensation, benefits and > relocation assistance. Most are DAY SHIFT!!! > They are ready to interview and hire NOW and you can start now or AFTER > the Holidays!! > > Without Further Ado here are the exciting opportunities I am currently > recruiting for: > > > Histology Leadership Opportunities: > Fayetteville, NC > > Histology Territory Sales Manager > Texas > OH > > Histotechnician/Histotechnologist ? DAYS Austin, TX Springfield, MA > Fayetteville, NC Norfolk, VA > > Histonetters, if you or anyone you know are interested in hearing more > about any of these opportunities or have another type of position or area > in mind and would like some help in your job search please let me know. > Just shoot me an e-mail at relia1 at earthlink.net or give me a call toll > free at > 866-607-3542 or on my cell at 407-353-5070. > > > And if you have a second I would love to hear what you would like the new > grads to know. Thanks again!!! > > > > > > > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > ?Pam M. Barker > ? > Pam Barker > President/Senior Recruiting Specialist-Histology RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell:???? (407)353-5070 > FAX:???? (407)678-2788 > E-mail: relia1 at earthlink.net > www.facebook.com/PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > > > > > > > > > > The information contained in this e-mail message may be privileged, > confidential, and/or protected from disclosure. This e-mail message may > contain protected health information (PHI); dissemination of PHI should > comply with applicable federal and state laws. If you are not the intended > recipient, or an authorized representative of the intended recipient, any > further review, disclosure, use, dissemination, distribution, or copying of > this message or any attachment (or the information contained therein) is > strictly prohibited. If you think that you have received this e-mail > message in error, please notify the sender by return e-mail and delete all > references to it and its contents from your systems. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Erin McCarthy, HT (ASCP) Histology Supervisor Tempus Labs 600 W. Chicago Ave. Chicago IL 60654 Ph:(312) 638-6344 Ext.3835 -- This email and any attachments may contain privileged and confidential information and/or protected health information (PHI) that is protected by federal and state privacy laws.? It is intended solely for the use of Tempus Labs and the recipient(s) named above.? Nothing contained in this communication and any attachments thereto is intended to waive any privileges or rights of confidentiality.? If you are not the recipient, or the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any review, dissemination, distribution, printing or copying of this email message and/or any attachments is strictly prohibited.?* If you have received this transmission in error, please notify us immediately at?**(855)-442-8305**? and permanently delete this email and any attachments*. From Finlay.Finlay at glasgow.ac.uk Thu Nov 8 08:43:27 2018 From: Finlay.Finlay at glasgow.ac.uk (Finlay Finlay) Date: Thu, 8 Nov 2018 14:43:27 +0000 Subject: [Histonet] Freezing artefact Message-ID: <0164C4117A8D594DBBF5265626928E39D9312A28@FORMED-ES.formed.internal> Good Afternoon all, I have a couple of cases where my post mortem samples have been accidentally frozen in Neutral Buffered Formalin. Naturally this has led to pretty striking artefact in the tissue. I was wondering if anyone who perhaps has more experience of dealing with frozen tissue has any tips on minimising this artefact? I still have some fixed tissue but am unable to resample from the unfixed tissue. Thanks in advance. Finlay Finlay Senior Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.finlay at glasgow.ac.uk The University of Glasgow Charity Number SC004401 Disclaimer: This e-mail is intended for the recipient only. If you are not the intended recipient you must not use, disclose, distribute, copy, print, or rely upon this e-mail. If an addressing or transmission error has misdirected this e-mail, please notify the author by replying to this e-mail and delete any local copy from your machine. E-mails are not necessarily secure. Forensic Medicine & Science (FMS) does not accept responsibility for changes made to this message after it was sent. Opinions, conclusions and other information in this message expressed by others or which do not relate to the official business of FMS shall be understood as neither given nor endorsed by FMS. It is expressly declared that this e-mail does not constitute nor form part of any contract or unilateral obligation unless the contrary is specifically stated in this e-mail and authorised by FMS. From amurvosh at advancederm.net Thu Nov 8 10:41:00 2018 From: amurvosh at advancederm.net (Anne Murvosh) Date: Thu, 8 Nov 2018 16:41:00 +0000 Subject: [Histonet] Gout specimens Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7BD6F85@Exchange.Advancederm.net> I haven't done a gout specimen in years, I remember the processing part, not remembering the Staining part. Could someone please send me your gout protocol? Thanks Anne Murvosh HT From tgenade at gmail.com Thu Nov 8 15:45:35 2018 From: tgenade at gmail.com (Tyrone Genade) Date: Thu, 8 Nov 2018 15:45:35 -0600 Subject: [Histonet] counter stains for Sudan Black B Message-ID: Hello, I need a counterstain for use together with Sudan Black B. I am using this method: https://www.ncbi.nlm.nih.gov/pubmed/27812872 which uses Nuclear Fast Red. I don't have nuclear fast red... In an updated protocol by these authors they use hematoxylin but in a previous trial I found it was too dark and make visualizing the Sudan Black signal too difficult. I'm using Sigma's Mayer's Hematoxylin solution. It is past its sell-by date and just won't blue nicely any more.. I have available Methyl Green, Neutral Red and Safranin O. The former washes out in aqueous/glycerol mounting medium (required for the protocol). How well does Neutral Red and Safranin work with an aqueous mounting medium? Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. *Don't panic if I don't reply to your emails sent over the weekend. I don't usually have time to check my email over the weekend and will get back to you on Monday.* From Lorraine.Smallwood at tiftregional.com Fri Nov 9 12:18:47 2018 From: Lorraine.Smallwood at tiftregional.com (Lorraine Smallwood) Date: Fri, 9 Nov 2018 18:18:47 +0000 Subject: [Histonet] Policy bariatric patient for autopsy Message-ID: <9B8F8973597D2847BD0476BE46B98D6F08521786@EXMDB01.trmc.us> Can anyone give me info on the CAP inspection packet regarding policy for bariatric patient for autopsy? I am a new "Histonet" junkie so any help from the experts will be greatly appreciated. This e-mail and any files transmitted with it may contain PRIVILEDGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the e-mail or any of its attachments, please be advised that you have received this e-mail in error and that use, dissemination, distribution, forwarding, printing, or copying of this e-mail or any attached files is strictly prohibited and may be illegal. If you have received this e-mail in error, please immediately purge it and all attachments and notify the sender by reply e-mail. From criley at dpspa.com Fri Nov 9 13:54:25 2018 From: criley at dpspa.com (Charles Riley) Date: Fri, 9 Nov 2018 14:54:25 -0500 Subject: [Histonet] Cost per test averages Message-ID: What is a good cost per test average for a standard H&E slide? I am trying to see if I should be fighting for better prices or if my current costs are below the average and am getting a good deal already. (Obviously the lower i can go the better Also what is your average cost per test for IHC's ? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From eddessa at emory.edu Fri Nov 9 14:10:22 2018 From: eddessa at emory.edu (Dessasau III, Evan) Date: Fri, 9 Nov 2018 20:10:22 +0000 Subject: [Histonet] Cost per test averages In-Reply-To: References: Message-ID: Hi Mr. Riley, would you mind sharing your cost? Thank you E-van -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, November 09, 2018 2:54 PM To: Histo List Subject: [Histonet] Cost per test averages What is a good cost per test average for a standard H&E slide? I am trying to see if I should be fighting for better prices or if my current costs are below the average and am getting a good deal already. (Obviously the lower i can go the better Also what is your average cost per test for IHC's ? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From rsrichmond at gmail.com Sat Nov 10 13:03:41 2018 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 10 Nov 2018 14:03:41 -0500 Subject: [Histonet] Policy bariatric patient for autopsy In-Reply-To: References: Message-ID: I don't understand Lorraine Smallwood's question: >>Can anyone give me info on the CAP inspection packet regarding policy for bariatric patient for autopsy? I am a new "Histonet" junkie so any help from the experts will be greatly appreciated.<< CAP inspections don't concern themselves with details of pathologic technique. Are you asking whether they have a policy for refusing autopsies on bodies so obese that the autopsy is difficult to do, or for performing the autopsy? Or a policy for what information to record in doing an autopsy on a patient who's had bariatric surgery? When I Googled "autopsy bariatric surgery" I found references for both of my questions. Bob Richmond Samurai Pathologist Maryville TN From john.frazier at roche.com Sat Nov 10 13:54:19 2018 From: john.frazier at roche.com (Frazier, John) Date: Sat, 10 Nov 2018 14:54:19 -0500 Subject: [Histonet] Cost per test averages Message-ID: I?m a vendor that sells an H&M staining product, so I?m not going to attempt to give you what the average cost per of a H&E stained slide. What I will tell you is that when looking at cost per slide you need to calculate more than your consumables. Those are going to be your capital cost. You also need to calculate in your labor cost. Those are gonna be your operational dollars. The reason why I say that is that some strainers are more efficient than other stainer. Not just in the staining process itself but in the overall maintenance, reagent management and waste control cost. Remember when using labor dollars you want to calculate that using fully burdens dollars. Be complete in the way that you calculate your overall cost per slide John Frazier, MBA, MT(ASCP) Strategic Workflow Consulting Roche Diagnostics > On Nov 9, 2018, at 14:54, Charles Riley wrote: > > What is a good cost per test average for a standard H&E slide? > > I am trying to see if I should be fighting for better prices or if my > current costs are below the average and am getting a good deal already. > (Obviously the lower i can go the better > > Also what is your average cost per test for IHC's ? > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > From Jennifer.Titus at greenwichhospital.org Sun Nov 11 15:27:13 2018 From: Jennifer.Titus at greenwichhospital.org (Titus, Jennifer) Date: Sun, 11 Nov 2018 21:27:13 +0000 Subject: [Histonet] Policy bariatric patient for autopsy In-Reply-To: References: , Message-ID: <5D311287A661CF4D9446D8791366A060BC6D771E@MBX13VP.YNHH.ORG> Yes, there is a relatively new guideline in the checklist regarding performance of bariatric autopsies, and you need a policy at your institution.... **NEW** 08/21/2017 ANP.34160 Safe Handling of Bariatric Patients Phase II There are written procedures for the special handling of autopsies on bariatric patients where the patient size could represent an occupational hazard to autopsy staff. NOTE: Individual institutions may set their own specific weight or BMI limits for application of the occupational health policy. Institutions may also choose whether to use special equipment for such patients and what type(s) of equipment to use. Evidence of Compliance: ? Written policy for handling of bariatric patients ________________________________________ From: Bob Richmond via Histonet [histonet at lists.utsouthwestern.edu] Sent: Saturday, November 10, 2018 2:03 PM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Policy bariatric patient for autopsy EXTERNAL EMAIL: Do NOT click links or open attachments unless you trust the sender AND know the content is safe. I don't understand Lorraine Smallwood's question: >>Can anyone give me info on the CAP inspection packet regarding policy for bariatric patient for autopsy? I am a new "Histonet" junkie so any help from the experts will be greatly appreciated.<< CAP inspections don't concern themselves with details of pathologic technique. Are you asking whether they have a policy for refusing autopsies on bodies so obese that the autopsy is difficult to do, or for performing the autopsy? Or a policy for what information to record in doing an autopsy on a patient who's had bariatric surgery? When I Googled "autopsy bariatric surgery" I found references for both of my questions. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message originates from the Yale New Haven Health System. The information contained in this message may be privileged and confidential. If you are the intended recipient you must maintain this message in a secure and confidential manner. If you are not the intended recipient, please notify the sender immediately and destroy this message. Thank you. From rsrichmond at gmail.com Sun Nov 11 17:24:19 2018 From: rsrichmond at gmail.com (Bob Richmond) Date: Sun, 11 Nov 2018 18:24:19 -0500 Subject: [Histonet] Policy bariatric patient for autopsy In-Reply-To: <5D311287A661CF4D9446D8791366A060BC6D771E@MBX13VP.YNHH.ORG> References: <5D311287A661CF4D9446D8791366A060BC6D771E@MBX13VP.YNHH.ORG> Message-ID: Well, it would have made a lot more sense if they'd said "morbidly obese patients" but I suppose that political correctness must be observed. If you don't have the equipment to do the autopsy safely, then you have to refuse to do the autopsy. If your pathologist doesn't have the authority to refuse the autopsy, then he may be putting his job on the line. Similar to this problem is the issue of refusing an autopsy on a patient with a hazardous infectious disease. Hepatitis and tuberculosis are the most common problems (AIDS really isn't). I was in trouble once for refusing an autopsy that didn't need doing, on a patient with hepatitis C, when the autopsy facility consisted of an ancient embalmer's table. If CAP offered any help here, I never heard of it. Bob Richmond Samurai Pathologist Maryville TN On Sun, Nov 11, 2018 at 4:27 PM Titus, Jennifer < Jennifer.Titus at greenwichhospital.org> wrote: > Yes, there is a relatively new guideline in the checklist regarding > performance of bariatric autopsies, and you need a policy at your > institution.... > > **NEW** 08/21/2017 > ANP.34160 Safe Handling of Bariatric Patients Phase II > There are written procedures for the special handling of autopsies on > bariatric patients > where the patient size could represent an occupational hazard to autopsy > staff. > NOTE: Individual institutions may set their own specific weight or BMI > limits for application of the > occupational health policy. Institutions may also choose whether to use > special equipment for > such patients and what type(s) of equipment to use. > Evidence of Compliance: > ? Written policy for handling of bariatric patients > > ________________________________________ > From: Bob Richmond via Histonet [histonet at lists.utsouthwestern.edu] > Sent: Saturday, November 10, 2018 2:03 PM > To: Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Policy bariatric patient for autopsy > > EXTERNAL EMAIL: Do NOT click links or open attachments unless you trust > the sender AND know the content is safe. > > > > > I don't understand Lorraine Smallwood's question: > > >>Can anyone give me info on the CAP inspection packet regarding policy for > bariatric patient for autopsy? I am a new "Histonet" junkie so any help > from the experts will be greatly appreciated.<< > > CAP inspections don't concern themselves with details of pathologic > technique. Are you asking whether they have a policy for refusing autopsies > on bodies so obese that the autopsy is difficult to do, or for performing > the autopsy? Or a policy for what information to record in doing an autopsy > on a patient who's had bariatric surgery? > > When I Googled "autopsy bariatric surgery" I found references for both of > my questions. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > This message originates from the Yale New Haven Health System. The > information contained in this message may be privileged and confidential. > If you are the intended recipient you must maintain this message in a > secure and confidential manner. If you are not the intended recipient, > please notify the sender immediately and destroy this message. Thank you. > From adesupo2002 at hotmail.com Sun Nov 11 21:48:35 2018 From: adesupo2002 at hotmail.com (ADESUPO ADESUYI) Date: Mon, 12 Nov 2018 03:48:35 +0000 Subject: [Histonet] Part time histotech job Message-ID: Hello, How are you guys going? I am looking for a part time histotech job in the San Francisco Bay area. I am both HT (ASCP)and HTL(ASCP) tech. I also possessed both QIHC (ASCP) and QLS(ASCP). I have an extensive years of experience in the field of histology and IHC. Thanks, Adesupo Sent from my MetroPCS 4G LTE Android Device From tbraud at holyredeemer.com Mon Nov 12 08:49:30 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 12 Nov 2018 14:49:30 +0000 Subject: [Histonet] Cost per test averages Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF875AC@HRHEX02-HOS.holyredeemer.local> I find it very peculiar to be lectured by a Roche representative on including reagent management when calculating stain costs, considering Roche's yearlong ongoing issues with reagent supply problems and massive recalls. I've yet to have one month with no reagent supply problems from Roche/Ventana. Failed dispensers, multiple recalls, major delays in deliveries of supplies (the most recent was a TWO MONTH BACKORDER on a routinely run antibody) and the list goes on. Roche needs to get their own house in order before they come on a technical list-serve to lecture. Just saying... Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: Message: 2 Date: Sat, 10 Nov 2018 14:54:19 -0500 From: "Frazier, John" To: Charles Riley I?m a vendor that sells an H&M staining product, so I?m not going to attempt to give you what the average cost per of a H&E stained slide. What I will tell you is that when looking at cost per slide you need to calculate more than your consumables. Those are going to be your capital cost. You also need to calculate in your labor cost. Those are gonna be your operational dollars. The reason why I say that is that some strainers are more efficient than other stainer. Not just in the staining process itself but in the overall maintenance, reagent management and waste control cost. Remember when using labor dollars you want to calculate that using fully burdens dollars. Be complete in the way that you calculate your overall cost per slide John Frazier, MBA, MT(ASCP) Strategic Workflow Consulting Roche Diagnostics From rjbuesa at yahoo.com Mon Nov 12 10:27:50 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Mon, 12 Nov 2018 16:27:50 +0000 (UTC) Subject: [Histonet] Cost per test averages In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8ECF875AC@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8ECF875AC@HRHEX02-HOS.holyredeemer.local> Message-ID: <1047480436.965973.1542040070173@mail.yahoo.com> Terri: All you wrote is absolutely true BUT has nothing to do with the "negotiating" aspect Charlie us asking about.He asked about the price he wants to pay and that will depend on his purchase volume that could determine, if his' is large enough, may entice the seller to reduce his profit margin. Ren? On Monday, November 12, 2018, 9:57:37 AM EST, Terri Braud via Histonet wrote: I find it very peculiar to be lectured by a Roche representative on including reagent management when calculating stain costs, considering Roche's yearlong ongoing issues with reagent supply problems and massive recalls.? I've yet to have one month with no reagent supply problems from Roche/Ventana.? Failed dispensers, multiple recalls, major delays in deliveries of supplies (the most recent was a TWO MONTH BACKORDER on a routinely run antibody) and the list goes on. Roche needs to get their own house in order before they come on a technical list-serve to lecture. Just saying... Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: Message: 2 Date: Sat, 10 Nov 2018 14:54:19 -0500 From: "Frazier, John" To: Charles Riley I?m a vendor that sells an H&M staining product, so I?m not going to attempt to give you what the average cost per of a H&E stained slide. What I will tell you is that when looking at cost per slide you need to calculate more than your consumables. Those are going to be your capital cost. You also need to calculate in your labor cost. Those are gonna be your operational dollars. The reason why I say that is that some strainers are more efficient than other stainer. Not just in the staining process itself but in the overall maintenance, reagent management and waste control cost. Remember when using labor dollars you want to calculate that using fully burdens dollars. Be complete in the way that you calculate your overall cost per slide John Frazier, MBA, MT(ASCP) Strategic Workflow Consulting Roche Diagnostics _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aeck at dh.org Mon Nov 12 10:25:02 2018 From: aeck at dh.org (Eck, Allison) Date: Mon, 12 Nov 2018 16:25:02 +0000 Subject: [Histonet] pathologic staging Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64AA7EDD5CB@DH-MAIL02.dhorg.org> Good morning histonet I have a question that is more for a pathologist but I am hoping some of you can help. When staging cancer cases (namely breast), there are 2 stagings, pathologic and clinical As per the AJCC, the pathologic staging includes information defined at surgery and clinical staging is determined by using information prior to surgery or neoadjuvant therapy. My question is: Are pathologists responsible for staging both the clinical and the pathologic stage? If not, do you know who does the clinical staging? Thanks Allison Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT),CEAS1 Lead Tech Histology Doylestown Hospital 595 W State St Doylestown, PA 18901 215-345-2264 aeck at dh.org From marktarango at gmail.com Mon Nov 12 12:35:18 2018 From: marktarango at gmail.com (Mark Tarango) Date: Mon, 12 Nov 2018 10:35:18 -0800 Subject: [Histonet] pathologic staging In-Reply-To: <4ED8C96A8F20FC4F883A92E2A0A0D64AA7EDD5CB@DH-MAIL02.dhorg.org> References: <4ED8C96A8F20FC4F883A92E2A0A0D64AA7EDD5CB@DH-MAIL02.dhorg.org> Message-ID: The pathologist should be responsible for pathologic staging on excisional specimens. The surgeon (sometimes oncologist) does the clinical staging. At least that is what happens at our local tumor board meeting. Mark On Mon, Nov 12, 2018 at 9:05 AM Eck, Allison via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good morning histonet > I have a question that is more for a pathologist but I am hoping some of > you can help. When staging cancer cases (namely breast), there are 2 > stagings, pathologic and clinical As per the AJCC, the pathologic staging > includes information defined at surgery and clinical staging is determined > by using information prior to surgery or neoadjuvant therapy. My question > is: Are pathologists responsible for staging both the clinical and the > pathologic stage? If not, do you know who does the clinical staging? > > Thanks > Allison > > Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT),CEAS1 > Lead Tech Histology > Doylestown Hospital > 595 W State St > Doylestown, PA 18901 > 215-345-2264 > aeck at dh.org > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Lindsay.A.Wilson at uth.tmc.edu Tue Nov 13 10:47:10 2018 From: Lindsay.A.Wilson at uth.tmc.edu (Wilson, Lindsay A) Date: Tue, 13 Nov 2018 16:47:10 +0000 Subject: [Histonet] Tissue Capture Pen Message-ID: Good morning, Has anyone used the Tissue Capture pen? If so, what are the reviews/results? Thanks, Lindsay Wilson, LVT, RLATG Young Laboratory UTHealth | The University of Texas Health Science Center at Houston | School of Dentistry Department of Oral & Maxillofacial Surgery 7500 Cambridge St. | Suite 6510 | Houston, TX 77054 713 486 4360 tel | 713 486 4333 fax From jvergara at micropathlabs.com Wed Nov 14 07:55:12 2018 From: jvergara at micropathlabs.com (Jacquilyn Vergara) Date: Wed, 14 Nov 2018 13:55:12 +0000 Subject: [Histonet] TMAs for MMR validation Message-ID: Will the US Biomax TMA CO804A show at least 10 cores with loss of staining for the MMR panel? Thanks! Jacquilyn R. Vergara MLS, HTL (ASCP)cm Histotechnologist/QA Coordinator MicroPath Laboratories, Inc. 1125 Bartow Rd., Ste. 101 Lakeland, FL 33801 WP:863-577-1147 NOTICE OF CONFIDENTIALITY The information in this email, including attachments, may be confidential and/or privileged and may contain confidential health information. This email is intended to be reviewed only by the individual or organization named as addressee. If you have received this email in error please notify MicroPath Laboratories immediately by return message to the sender or to info at micropathlabs.com. Destroy all copies of this message and any attachments. Please note that any views or opinions presented in this email are solely those of the author and do not necessarily represent those of MicroPath Laboratories. Confidential health information is protected by state and federal law, including, but not limited to: The Health Insurance Portability and Accountability Act of 1996 and related regulations. From relia1 at earthlink.net Wed Nov 14 09:51:25 2018 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 14 Nov 2018 10:51:25 -0500 Subject: [Histonet] Posting for a friend! Histopeeps!! Know any Cytopeeps looking for some Temp Work? Message-ID: <00ea01d47c31$e6792dd0$b36b8970$@earthlink.net> Hi Pam, Thanks for your help with this. See below. Please copy and paste. Full Time temporary Cytotech Pathologists Diagnostic Laboratory a private pathology laboratory located in downtown Winston Salem, is currently seeking a Cytotech to fill a temporary full-time position. This is a first shift position Monday to Friday with hours that are negotiable. Timeframe ? 8-10 weeks (Dec17 thru Feb 18) General Responsibilities ? Screening Gyn/Non-Gyn specimens ? Accession/Process Gyn samples ? Perform molecular testing using automated analyzer (training provided) ? Maintain workload records Qualifications ? CT ASCP certified ? Molecular experience preferred, not required ? 1-3 years experience For more information contact: Cheryl Morrison Human Resources Manager Pathologists Diagnostic Laboratory, PA (336)306-5770 cpmorrison at pdlpath.com Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Katie at PuyallupDerm.com Wed Nov 14 11:16:58 2018 From: Katie at PuyallupDerm.com (Katie) Date: Wed, 14 Nov 2018 09:16:58 -0800 Subject: [Histonet] MART-1 immunot procedure Message-ID: <94B21BF296BC4690B9AE899F23C731F9@LabHP> Hello all, My dermpath lab is looking into adding a MART-1 immuno, as a hand stain. The procedure that I received from BioCare for their cocktail kit is extremely minimal. Do any of you have a more thorough and/or broken down hand stain SOP that I could please reference? Thank you so much, Katie Riley-Hamilton Technical Supervisor of Dermatopathology Puyallup Dermatology Clinic katie at puyallupderm.com From tbraud at holyredeemer.com Wed Nov 14 12:56:17 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 14 Nov 2018 18:56:17 +0000 Subject: [Histonet] BioCare mart 1 Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF87F40@HRHEX02-HOS.holyredeemer.local> Just call BioCare Technical service. They have a complete procedure for hand staining. I know because I used it in their facility when I went for training on their Flx. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 3. MART-1 immunot procedure (Katie) ------------------------------ Message: 3 Date: Wed, 14 Nov 2018 09:16:58 -0800 From: "Katie" Subject: [Histonet] MART-1 immunot procedure Hello all, My dermpath lab is looking into adding a MART-1 immuno, as a hand stain. The procedure that I received from BioCare for their cocktail kit is extremely minimal. Do any of you have a more thorough and/or broken down hand stain SOP that I could please reference? Thank you so much, Katie Riley-Hamilton Technical Supervisor of Dermatopathology Puyallup Dermatology Clinic katie at puyallupderm.com From Monica.Lockhart at luhs.org Wed Nov 14 14:24:20 2018 From: Monica.Lockhart at luhs.org (MONICA D. LOCKHART) Date: Wed, 14 Nov 2018 20:24:20 +0000 Subject: [Histonet] Inconsistent H & E Staining Message-ID: Hello Histo Fam!! Please help! My facility is experiencing frequent H & E staining problems. Everything from cloudy cells on routine GI to "The Blank Look" on PNB's. We have made changes in some of the obvious areas like cassette whole size and discontinued the use of sponges at grossing. However, with our biopsies, we still see varying results in our H&E staining. Though this is not an exhaustive list of all the things we have encountered or the number of changes we have made, hopefully this will give you an idea of what we are seeing and maybe you have ran across this problem and can share your wisdom. Any comments would be appreciated and most welcome. Monica D. Lockhart, BBA, HT (ASCP) PBT Supervisor Clinical Labs Histology Loyola University Medical Center 2160 S. First Ave, Bldg 110 Rm 2290 Maywood, IL 60153 (o) 708.327.2608 (c) 708.692.8361 monica.lockhart at luhs.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From allyse124 at gmail.com Wed Nov 14 14:28:49 2018 From: allyse124 at gmail.com (Allyse Mazzarelli) Date: Wed, 14 Nov 2018 15:28:49 -0500 Subject: [Histonet] Help with frozen spleen and liver tissue! Message-ID: Hi all, Can someone please provide me with more details regarding cryosectioning of mouse spleen and liver tissue? Currently, I've been fixing my samples in 4% PFA (they are well fixed - I know that will be the first question asked!), and then cryoprotect the tissue in a series of graded sucrose solutions (15% to 30%) until they sink. However, when I go to place the sections on the slide from the cryostat, they look great initially under the microscope, but once they dry they have poor morphology, especially seen in the liver. I've never run into this issue before, with either brain or spinal cord which are significantly more delicate. I've tried cutting the tissue free-floating as well to see if it was how I was placing the sections on the slide, but nothing seems to work. The morphology in the liver is so terribly compromised that I cannot visualize the sinusoids properly. It is baffling that once they go onto the slide they look okay, but 5 minutes later the tissue appears to "separate" from itself. Does anyone work specifically in frozen tissue sections, liver and spleen in particular? If so, would you be able to help me figure out the best way to generate quality specimens? Thank you! Regards, Allyse Mazzarelli From mills at 3scan.com Wed Nov 14 14:50:46 2018 From: mills at 3scan.com (Caroline Miller) Date: Wed, 14 Nov 2018 12:50:46 -0800 Subject: [Histonet] Inconsistent H & E Staining In-Reply-To: References: Message-ID: Have you checked your dewaxing solutions? I have sometimes seen this when slides are not fully dewaxed. yours, mills ? On Wed, Nov 14, 2018 at 12:34 PM MONICA D. LOCKHART via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello Histo Fam!! > > Please help! > > My facility is experiencing frequent H & E staining problems. Everything > from cloudy cells on routine GI to "The Blank Look" on PNB's. > > We have made changes in some of the obvious areas like cassette whole size > and discontinued the use of sponges at grossing. However, with our > biopsies, we still see varying results in our H&E staining. > > Though this is not an exhaustive list of all the things we have > encountered or the number of changes we have made, hopefully this will give > you an idea of what we are seeing and maybe you have ran across this > problem and can share your wisdom. > > Any comments would be appreciated and most welcome. > > > Monica D. Lockhart, BBA, HT (ASCP) PBT > Supervisor Clinical Labs Histology > Loyola University Medical Center > 2160 S. First Ave, Bldg 110 Rm 2290 > Maywood, IL 60153 > (o) 708.327.2608 > (c) 708.692.8361 > monica.lockhart at luhs.org > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Biology www.3Scan.com 415 2187297 From e.kooijman at vumc.nl Thu Nov 15 04:53:04 2018 From: e.kooijman at vumc.nl (Kooijman, E.J.M. (Esther)) Date: Thu, 15 Nov 2018 10:53:04 +0000 Subject: [Histonet] p2y12 cryo rat problem high background Message-ID: <51AEA3C85E64ED4E851909E3CCAB574C014551B321@sp-mx-mbx2> Hello all, I am trying to stain cryo brain sections from the rat 7um but having a lot of background. What am I doing wrong. Below the protocol is used and tried to adjust? 1- Fix the tissue with cold acetone (-20 ?C) for 10 minutes 2- Let the slide dry for 30 minutes at room temperature. Isolate the sections with DAKO pen 3- Block sections with BSA 2% in PBS for 1 hour at room temperature 4- Add primary antibody in PBS/BSA 1%, overnight at 4?C or 1h at room temperature 5- Wash 3x5 minutes with PBS/tween-20 0.05% 6- Add 100 ?L Envision solution (goat anti-mouse/rabbit) and incubate for 1hr at RT 7- Wash 3x 5 minutes with PBS/tween-20 0.05% 8- Incubate with DAB (1:50) for 10 min (between 5-10 min; check color development) (wear gloves, carcinogenic!) 9- Rinse thoroughly with miliQ water 10- Stain with haematoxylin for 1 min 11- Wash with running tap water for 5 min 12- Start the alcohol/xylene series (70% EtOH -> 80% EtOH -> 96% EtOH -> 100% EtOH -> 100% EtOH/xylene -> xylene -> xylene) 13- Mount slides with entallan Kind regards, Esther Kooijman | Research Technician | Department of Radiology and Nuclear medicine The Netherlands From e.kooijman at vumc.nl Thu Nov 15 06:44:35 2018 From: e.kooijman at vumc.nl (Kooijman, E.J.M. (Esther)) Date: Thu, 15 Nov 2018 12:44:35 +0000 Subject: [Histonet] p2y12 cryo rat problem high background In-Reply-To: References: <51AEA3C85E64ED4E851909E3CCAB574C014551B321@sp-mx-mbx2> Message-ID: <51AEA3C85E64ED4E851909E3CCAB574C014551B38B@sp-mx-mbx2> Hello Bobbie, But should I elimination endogenous peroxidase activity in brain/spinal cord tissues? Brains and spinal cord was harvested after cervical dislocation, then the tissue was snap frozen (isopentane..). thanks for your help, Esther -----Oorspronkelijk bericht----- Van: Boyce, Bobbie [mailto:Bobbie.Boyce at nemours.org] Verzonden: donderdag 15 november 2018 12:21 Aan: Kooijman, E.J.M. (Esther) Onderwerp: RE: p2y12 cryo rat problem high background Hi Ester, Try Peroxoblock (Zymed) before your BSA block, but you have to be careful not to leave it on too long or it will eat your tissue. It's been a while since I've had to used it. Bobbie Boyce Histology Specialist III DuPont Experimental Station Nemours- Biomedical Research Department Histochemistry and Tissue Processing Core 200 Powder Mill Road, Bldg.400 Rm.5240 Wilmington, DE 19803 (lab) 302-651-6771 (fax) 302-651-5010 -----Original Message----- From: Kooijman, E.J.M. (Esther) via Histonet Sent: Thursday, November 15, 2018 5:53 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] p2y12 cryo rat problem high background **This is an External Email - Please DO NOT open attachments or click links from unknown senders or unexpected email. ** Hello all, I am trying to stain cryo brain sections from the rat 7um but having a lot of background. What am I doing wrong. Below the protocol is used and tried to adjust? 1- Fix the tissue with cold acetone (-20 ?C) for 10 minutes 2- Let the slide dry for 30 minutes at room temperature. Isolate the sections with DAKO pen 3- Block sections with BSA 2% in PBS for 1 hour at room temperature 4- Add primary antibody in PBS/BSA 1%, overnight at 4?C or 1h at room temperature 5- Wash 3x5 minutes with PBS/tween-20 0.05% 6- Add 100 ?L Envision solution (goat anti-mouse/rabbit) and incubate for 1hr at RT 7- Wash 3x 5 minutes with PBS/tween-20 0.05% 8- Incubate with DAB (1:50) for 10 min (between 5-10 min; check color development) (wear gloves, carcinogenic!) 9- Rinse thoroughly with miliQ water 10- Stain with haematoxylin for 1 min 11- Wash with running tap water for 5 min 12- Start the alcohol/xylene series (70% EtOH -> 80% EtOH -> 96% EtOH -> 100% EtOH -> 100% EtOH/xylene -> xylene -> xylene) 13- Mount slides with entallan Kind regards, Esther Kooijman | Research Technician | Department of Radiology and Nuclear medicine The Netherlands _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=X2IGR6v8ax_mLhSmU1r3Aw&r=NAc-sZTcJtcdymsLif_fp7ngB_dNBpF-UI64u9fuosc&m=N05bQ_qJ2Li62f83kZl-FK7rp5Ad-spj9JTvwovnnKQ&s=6MYtZ-ICVPPxaohoUjidJjm_mNhwB7nDA1Np5SRxT4w&e= From allyse124 at gmail.com Thu Nov 15 07:25:49 2018 From: allyse124 at gmail.com (Allyse Mazzarelli) Date: Thu, 15 Nov 2018 08:25:49 -0500 Subject: [Histonet] p2y12 cryo rat problem high background In-Reply-To: <51AEA3C85E64ED4E851909E3CCAB574C014551B38B@sp-mx-mbx2> References: <51AEA3C85E64ED4E851909E3CCAB574C014551B321@sp-mx-mbx2> <51AEA3C85E64ED4E851909E3CCAB574C014551B38B@sp-mx-mbx2> Message-ID: Hi Esther, I worked extensively with brain and spinal cord sections in the past, in multiple species. You need endogenous quenching step. I would always make my own (commerically available components yielded different results). I used a 0.09% H2O2 in 6% Triton-X 100: (formula: 200mL 1x PBS + 6mL H2O2 + 0.33mL 6% Triton-X 100 solution). Are you samples fixed frozen or fresh frozen? If fixed-frozen, there is no need for the acetone step. In fact, acetone in brain and spinal cord sections makes the tissue brittle. I would recommend this is skipped. If your samples are fresh-frozen, I would recommend a 95% EtOH fix. This is significantly gentler on the tissue. I have no experience with the Envision system, but the polymer system I would always use was from Cell Signaling Technologies. What antibody are you using? On Thu, Nov 15, 2018 at 7:48 AM Kooijman, E.J.M. (Esther) via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello Bobbie, > > But should I elimination endogenous peroxidase activity in brain/spinal > cord tissues? > Brains and spinal cord was harvested after cervical dislocation, then the > tissue was snap frozen (isopentane..). > > thanks for your help, > Esther > > -----Oorspronkelijk bericht----- > Van: Boyce, Bobbie [mailto:Bobbie.Boyce at nemours.org] > Verzonden: donderdag 15 november 2018 12:21 > Aan: Kooijman, E.J.M. (Esther) > Onderwerp: RE: p2y12 cryo rat problem high background > > Hi Ester, > Try Peroxoblock (Zymed) before your BSA block, but you have to be careful > not to leave it on too long or it will eat your tissue. It's been a while > since I've had to used it. > > > Bobbie Boyce > Histology Specialist III > DuPont Experimental Station > Nemours- Biomedical Research Department > Histochemistry and Tissue Processing Core > 200 Powder Mill Road, Bldg.400 Rm.5240 > Wilmington, DE 19803 > > (lab) 302-651-6771 > (fax) 302-651-5010 > > > > -----Original Message----- > From: Kooijman, E.J.M. (Esther) via Histonet < > histonet at lists.utsouthwestern.edu> > Sent: Thursday, November 15, 2018 5:53 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] p2y12 cryo rat problem high background > > **This is an External Email - Please DO NOT open attachments or click > links from unknown senders or unexpected email. ** > > Hello all, > > > > I am trying to stain cryo brain sections from the rat 7um but having a lot > of background. What am I doing wrong. Below the protocol is used and tried > to adjust? > > > > 1- Fix the tissue with cold acetone (-20 ?C) for 10 minutes > > > > 2- Let the slide dry for 30 minutes at room temperature. Isolate the > sections with DAKO pen > > > > 3- Block sections with BSA 2% in PBS for 1 hour at room temperature > > > > 4- Add primary antibody in PBS/BSA 1%, overnight at 4?C or 1h at room > temperature > > > > 5- Wash 3x5 minutes with PBS/tween-20 0.05% > > > > 6- Add 100 ?L Envision solution (goat anti-mouse/rabbit) and incubate > for 1hr at RT > > > > 7- Wash 3x 5 minutes with PBS/tween-20 0.05% > > > > 8- Incubate with DAB (1:50) for 10 min (between 5-10 min; check color > development) > > (wear gloves, carcinogenic!) > > > > 9- Rinse thoroughly with miliQ water > > > > 10- Stain with haematoxylin for 1 min > > > > 11- Wash with running tap water for 5 min > > > > 12- Start the alcohol/xylene series (70% EtOH -> 80% EtOH -> 96% EtOH -> > 100% EtOH -> 100% EtOH/xylene -> xylene -> xylene) > > > > 13- Mount slides with entallan > > Kind regards, > > > > > > Esther Kooijman | Research Technician | Department of Radiology and > Nuclear medicine > > The Netherlands > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=X2IGR6v8ax_mLhSmU1r3Aw&r=NAc-sZTcJtcdymsLif_fp7ngB_dNBpF-UI64u9fuosc&m=N05bQ_qJ2Li62f83kZl-FK7rp5Ad-spj9JTvwovnnKQ&s=6MYtZ-ICVPPxaohoUjidJjm_mNhwB7nDA1Np5SRxT4w&e= > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From patpxs at gmail.com Thu Nov 15 08:59:31 2018 From: patpxs at gmail.com (Patpxs) Date: Thu, 15 Nov 2018 06:59:31 -0800 Subject: [Histonet] p2y12 cryo rat problem high background In-Reply-To: <51AEA3C85E64ED4E851909E3CCAB574C014551B38B@sp-mx-mbx2> References: <51AEA3C85E64ED4E851909E3CCAB574C014551B321@sp-mx-mbx2> <51AEA3C85E64ED4E851909E3CCAB574C014551B38B@sp-mx-mbx2> Message-ID: It could be the concentration of the antibody is too high. Have you tried a lower dilution? What species made the antibody? I know that sounds a bit basic but I know that I have used a mouse or rat antibody by mistake. Paula Sent from my iPhone > On Nov 15, 2018, at 4:44 AM, Kooijman, E.J.M. (Esther) via Histonet wrote: > > Hello Bobbie, > > But should I elimination endogenous peroxidase activity in brain/spinal cord tissues? > Brains and spinal cord was harvested after cervical dislocation, then the tissue was snap frozen (isopentane..). > > thanks for your help, > Esther > > -----Oorspronkelijk bericht----- > Van: Boyce, Bobbie [mailto:Bobbie.Boyce at nemours.org] > Verzonden: donderdag 15 november 2018 12:21 > Aan: Kooijman, E.J.M. (Esther) > Onderwerp: RE: p2y12 cryo rat problem high background > > Hi Ester, > Try Peroxoblock (Zymed) before your BSA block, but you have to be careful not to leave it on too long or it will eat your tissue. It's been a while since I've had to used it. > > > Bobbie Boyce > Histology Specialist III > DuPont Experimental Station > Nemours- Biomedical Research Department > Histochemistry and Tissue Processing Core > 200 Powder Mill Road, Bldg.400 Rm.5240 > Wilmington, DE 19803 > > (lab) 302-651-6771 > (fax) 302-651-5010 > > > > -----Original Message----- > From: Kooijman, E.J.M. (Esther) via Histonet > Sent: Thursday, November 15, 2018 5:53 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] p2y12 cryo rat problem high background > > **This is an External Email - Please DO NOT open attachments or click links from unknown senders or unexpected email. ** > > Hello all, > > > > I am trying to stain cryo brain sections from the rat 7um but having a lot of background. What am I doing wrong. Below the protocol is used and tried to adjust? > > > > 1- Fix the tissue with cold acetone (-20 ?C) for 10 minutes > > > > 2- Let the slide dry for 30 minutes at room temperature. Isolate the sections with DAKO pen > > > > 3- Block sections with BSA 2% in PBS for 1 hour at room temperature > > > > 4- Add primary antibody in PBS/BSA 1%, overnight at 4?C or 1h at room temperature > > > > 5- Wash 3x5 minutes with PBS/tween-20 0.05% > > > > 6- Add 100 ?L Envision solution (goat anti-mouse/rabbit) and incubate for 1hr at RT > > > > 7- Wash 3x 5 minutes with PBS/tween-20 0.05% > > > > 8- Incubate with DAB (1:50) for 10 min (between 5-10 min; check color development) > > (wear gloves, carcinogenic!) > > > > 9- Rinse thoroughly with miliQ water > > > > 10- Stain with haematoxylin for 1 min > > > > 11- Wash with running tap water for 5 min > > > > 12- Start the alcohol/xylene series (70% EtOH -> 80% EtOH -> 96% EtOH -> 100% EtOH -> 100% EtOH/xylene -> xylene -> xylene) > > > > 13- Mount slides with entallan > > Kind regards, > > > > > > Esther Kooijman | Research Technician | Department of Radiology and Nuclear medicine > > The Netherlands > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=X2IGR6v8ax_mLhSmU1r3Aw&r=NAc-sZTcJtcdymsLif_fp7ngB_dNBpF-UI64u9fuosc&m=N05bQ_qJ2Li62f83kZl-FK7rp5Ad-spj9JTvwovnnKQ&s=6MYtZ-ICVPPxaohoUjidJjm_mNhwB7nDA1Np5SRxT4w&e= > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurizaker at gmail.com Thu Nov 15 09:11:06 2018 From: laurizaker at gmail.com (Lauri Zaker) Date: Thu, 15 Nov 2018 07:11:06 -0800 Subject: [Histonet] Cryosectioning Brain problem Message-ID: Hello, I am wondering if anyone has any tips on how to get smooth sections of fixed frozen sections of mouse brain. We are getting small wrinkles throughout. Thanks for any input. From tgenade at gmail.com Thu Nov 15 12:23:13 2018 From: tgenade at gmail.com (Tyrone Genade) Date: Thu, 15 Nov 2018 12:23:13 -0600 Subject: [Histonet] Histonet Digest, Vol 180, Issue 7 In-Reply-To: References: Message-ID: Hello, An update on my question: On Fri, Nov 9, 2018 at 12:22 PM wrote: > > Message: 1 > Date: Thu, 8 Nov 2018 15:45:35 -0600 > From: Tyrone Genade > To: histonet > Subject: [Histonet] counter stains for Sudan Black B > Message-ID: > < > CAEYEE3nxb7nWezRbL_UODTnTJtqiDhRyxkSdmgv4kvMv_pqpuw at mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hello, > > I need a counterstain for use together with Sudan Black B. I am using this > method: https://www.ncbi.nlm.nih.gov/pubmed/27812872 which uses Nuclear > Fast Red. I don't have nuclear fast red... In an updated protocol by these > authors they use hematoxylin but in a previous trial I found it was too > dark and make visualizing the Sudan Black signal too difficult. I'm using > Sigma's Mayer's Hematoxylin solution. It is past its sell-by date and just > won't blue nicely any more.. > > I have available Methyl Green, Neutral Red and Safranin O. The former > washes out in aqueous/glycerol mounting medium (required for the protocol). > How well does Neutral Red and Safranin work with an aqueous mounting > medium? > There were no replies but Neutral Red worked. Does anyone know of a paper describing the cellular structures stained by Neutral red? https://en.wikipedia.org/wiki/Neutral_red states that is stains the Golgi apparatus and Nissl bodies and that living cells take up the stain in lysosomes. http://stainsfile.info/StainsFile/stain/nuclei/neutred.htm says it stains the nucleus. I see a darker stained red halo around the nucleus and the nucleus is stained reddish. It also seems to stain the aggresome, that is it stains small structures juxtaposed to the nucleus. The Sudan Black seems to overshadow the stain. Any speculation what is going on? Is the neutral red staining structures that would also contain lipofuscin? Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. *Don't panic if I don't reply to your emails sent over the weekend. I don't usually have time to check my email over the weekend and will get back to you on Monday.* From john.garratt at ciqc.ca Thu Nov 15 12:31:34 2018 From: john.garratt at ciqc.ca (John Garratt) Date: Thu, 15 Nov 2018 18:31:34 +0000 Subject: [Histonet] Inconsistent H & E Staining In-Reply-To: References: Message-ID: A quick question: Are you using any recycled reagents? ie xylene, alcohol John www.ciqc.ca ??????? Original Message ??????? On Wednesday, November 14, 2018 12:24 PM, MONICA D. LOCKHART via Histonet wrote: > Hello Histo Fam!! > > Please help! > > My facility is experiencing frequent H & E staining problems. Everything from cloudy cells on routine GI to "The Blank Look" on PNB's. > > We have made changes in some of the obvious areas like cassette whole size and discontinued the use of sponges at grossing. However, with our biopsies, we still see varying results in our H&E staining. > > Though this is not an exhaustive list of all the things we have encountered or the number of changes we have made, hopefully this will give you an idea of what we are seeing and maybe you have ran across this problem and can share your wisdom. > > Any comments would be appreciated and most welcome. > > Monica D. Lockhart, BBA, HT (ASCP) PBT > Supervisor Clinical Labs Histology > Loyola University Medical Center > 2160 S. First Ave, Bldg 110 Rm 2290 > Maywood, IL 60153 > (o) 708.327.2608 > (c) 708.692.8361 > monica.lockhart at luhs.org > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mwich at 7thwavelabs.com Thu Nov 15 13:23:36 2018 From: mwich at 7thwavelabs.com (Michele Wich) Date: Thu, 15 Nov 2018 19:23:36 +0000 Subject: [Histonet] IHC control slides for EBNA Message-ID: <4ADAF16E8518764ABF9DCEB73129AA7586155428@WAVE-EMAIL.7thwave.local> Does anyone know of a source of IHC control slides for EBV Nuclear Antigen? Thank you. Michele From regan.fulton at gmail.com Thu Nov 15 13:38:37 2018 From: regan.fulton at gmail.com (Fulton Regan) Date: Thu, 15 Nov 2018 11:38:37 -0800 Subject: [Histonet] EBV control slides Message-ID: <3CDE55A0-721B-4511-9E37-0AA35149A4DF@gmail.com> Michele, Hope this helps! http://www.biosb.com/biosb-products/infectious-disease-arrays/ BSB 0237 Epstein Barr Virus Cell Line Array (2 Core) 5 slides Regan Fulton, M.D., Ph.D. CEO, Array Science, LLC 475 Gate 5 Road, #102 Sausalito, CA 94965 From gordon at 10db.co.uk Thu Nov 15 14:56:30 2018 From: gordon at 10db.co.uk (Gordon Brown) Date: Thu, 15 Nov 2018 20:56:30 -0000 Subject: [Histonet] Detached vinyl coverslips - any advice on reattachment? Message-ID: I'm an amateur microscopist and along with a couple of fellow club members we have acquired a fairly extensive collection of histopathology slides (approx. 2500 slides) made in the early 2000s and used for seminar and lecture purposes. We intend to use these to further our knowledge and interest in histopathology and the majority of the slides are in superb condition for this purpose and reasonably well documented. However, approximately 300 to 400 of the slides are suffering from detachment of the coverslip, these coverslips being made of what I assume to be vinyl, and we are attempting to reattach them. Can anyone advise how this type of coverslip was attached in the first instance? I'm assuming this was done using an automatic coverslipper, but there's no easily visible trace of mountant on either the slide or the coverslip so they may be self-adhesive or use a heat activated adhesive. Each coverslip is 55.4mm x 24.1mm. Hope someone can help, if we know how they were attached in the first instance this may give us some ideas for a strategy for reattachment. Regards Gordon From jmcgough at clinlab.com Thu Nov 15 15:18:40 2018 From: jmcgough at clinlab.com (=?utf-8?Q?Jason_McGough?=) Date: Thu, 15 Nov 2018 14:18:40 -0700 Subject: [Histonet] Histology Supervisor Position In-Reply-To: References: Message-ID: Clinical Laboratory of the Black Hills is an independent pathology practice providing anatomic and cytologic services to Rapid City, South Dakota and surrounding communities. Rapid City is the gateway to the Black Hills and offers a variety of four season, family friendly activities. Our histology department processes 25,000 surgical cases, and 200 autopsies per year. We also have a progressive IHC department, and perform a variety of special stains and frozen sections. HISTOLOGY SUPERVISOR Candidate must demonstrate strong leadership skills in areas of developing, training, and motivating staff. Requirements are HT/HTL certification and 2 years of successful management experience. Functions include oversight, supervision, and coordination of all histology activities for a department of 9 employees. Assists Operations Manager in developing, implementing, revising, interpreting, and enforcing standard operating procedures, company policies, and service standards. Assists with purchasing and budgeting for the histology department. Participates on the quality assurance committee. Oversees scheduling, employee time off requests, and evaluations. Helps maintain turnaround time expectations and performs other tasks as assigned. Clinical Lab offers a competitive wage with an excellent benefit package including health and dental insurance, 401(k), Profit Sharing, and long-term disability insurance. No state income tax. Relocation assistance available. Send resume to: Janet Amundson, Human Resources Clinical Laboratory of the Black Hills 2805 5th Street, Suite 210 Rapid City, South Dakota 57701 Fax: 605-342-0418; Phone: 605-343-2267 Email: jamundson at clinlab.com Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills Main: 605-343-2267 Direct: 605-716-4206 jmcgough at clinlab.com www.clinlab.com From gordon at 10db.co.uk Thu Nov 15 16:13:14 2018 From: gordon at 10db.co.uk (Gordon Brown) Date: Thu, 15 Nov 2018 22:13:14 +0000 Subject: [Histonet] Detached vinyl coverslips - any advice on reattachment? Message-ID: I should have mentioned that the section invariably remains attached to these vinyl coverslips when they become detached from the slides. If they had remained on the slides we wouldn't have a problem as we are used to restoring much older, Victorian slides that use Canada Balsam as a mountant. In this instance our options are obviously limited by lack of understanding how the coverslips are applied and that coverslipping sysrem works. Gordon -------- Original message -------- From: Paula Keene Pierce Date:15/11/2018 21:45 (GMT+00:00) To: Gordon Brown Subject: Re: [Histonet] Detached vinyl coverslips - any advice on reattachment? Hi, you will need to soak those in acetone to remove the plastic coverslip. Then you can coverslip as usual with glass and resin. Paula Keene Pierce, BS, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Drive Norman, OK 73069 PH 405-759-3953 www.excaliburpathology.com From: Gordon Brown via Histonet To: histonet at lists.utsouthwestern.edu Sent: Thursday, November 15, 2018 3:15 PM Subject: [Histonet] Detached vinyl coverslips - any advice on reattachment? I'm an amateur microscopist and along with a couple of fellow club members we have acquired a fairly extensive collection of histopathology slides (approx. 2500 slides) made in the early 2000s and used for seminar and lecture purposes. We intend to use these to further our knowledge and interest in histopathology and the majority of the slides are in superb condition for this purpose and reasonably well documented. However, approximately 300 to 400 of the slides are suffering from detachment of the coverslip, these coverslips being made of what I assume to be vinyl, and we are attempting to reattach them. Can anyone advise how this type of coverslip was attached in the first instance? I'm assuming this was done using an automatic coverslipper, but there's no easily visible trace of mountant on either the slide or the coverslip so they may be self-adhesive or use a heat activated adhesive. Each coverslip is 55.4mm x 24.1mm. Hope someone can help, if we know how they were attached in the first instance this may give us some ideas for a strategy for reattachment. Regards Gordon _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmcampbe at fmh.org Fri Nov 16 09:25:21 2018 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Fri, 16 Nov 2018 15:25:21 +0000 Subject: [Histonet] test Message-ID: I keep trying to send an email for help with an issue I am having and its not going through. It says the email has been sent but I am not getting the email and no one has responded. I am testing to see if this message goes through. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 CONFIDENTIALITY NOTICE: This electronic mail transmission and any accompanying data files is confidential and is intended exclusively for the individual or entity to which it is addressed. The communication may contain information that is proprietary, privileged or confidential or otherwise legally exempt from disclosure. If you are not the named addressee or you otherwise have received this message in error, you are not authorized to read, print, copy or disseminate this message or any part of it. If you have received this message in error, please notify the sender immediately by email and delete all copies of this message. Receipt by anyone other than the named addressee is not a waiver of any attorney-client work product or other applicable privilege. From akemiat3377 at gmail.com Fri Nov 16 10:00:54 2018 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Fri, 16 Nov 2018 08:00:54 -0800 Subject: [Histonet] test In-Reply-To: References: Message-ID: <55DE4F3E-8101-43F5-84AF-0FEF18FA3545@gmail.com> I received it. I have had the same problem lately. I didn?t get a copy of the email sent. It would be nice to know that it was received. Think they changed their format. Akemi Allison > On Nov 16, 2018, at 7:25 AM, Campbell, Tasha M. via Histonet wrote: > > I keep trying to send an email for help with an issue I am having and its not going through. It says the email has been sent but I am not getting the email and no one has responded. I am testing to see if this message goes through. > > > > > Tasha Campbell, B.S.,HTL(ASCP) > Frederick Gastroenterology Associates > 310 W. 9th St. > Frederick, MD 21701 > 301-695-6800 ext. 144 > > > CONFIDENTIALITY NOTICE: This electronic mail transmission and any accompanying data files is confidential and is intended exclusively for the individual or entity to which it is addressed. The communication may contain information that is proprietary, privileged or confidential or otherwise legally exempt from disclosure. If you are not the named addressee or you otherwise have received this message in error, you are not authorized to read, print, copy or disseminate this message or any part of it. If you have received this message in error, please notify the sender immediately by email and delete all copies of this message. Receipt by anyone other than the named addressee is not a waiver of any attorney-client work product or other applicable privilege. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmcampbe at fmh.org Fri Nov 16 10:11:56 2018 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Fri, 16 Nov 2018 16:11:56 +0000 Subject: [Histonet] test In-Reply-To: <55DE4F3E-8101-43F5-84AF-0FEF18FA3545@gmail.com> References: <55DE4F3E-8101-43F5-84AF-0FEF18FA3545@gmail.com> Message-ID: Thanks. So I got a copy of this email I sent. But I sent my question twice and did not get the copy. I put a screen shot in the message. Could that mess it up? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 -----Original Message----- From: Eileen Akemi Allison [mailto:akemiat3377 at gmail.com] Sent: Friday, November 16, 2018 11:01 AM To: Campbell, Tasha M.; Histonet Subject: Re: [Histonet] test ** CAUTION: This email originated from outside of Frederick Memorial Hospital. DO NOT click on links or open attachments if you do not recognize the sender. ** I received it. I have had the same problem lately. I didn't get a copy of the email sent. It would be nice to know that it was received. Think they changed their format. Akemi Allison > On Nov 16, 2018, at 7:25 AM, Campbell, Tasha M. via Histonet wrote: > > I keep trying to send an email for help with an issue I am having and its not going through. It says the email has been sent but I am not getting the email and no one has responded. I am testing to see if this message goes through. > > > > > Tasha Campbell, B.S.,HTL(ASCP) > Frederick Gastroenterology Associates > 310 W. 9th St. > Frederick, MD 21701 > 301-695-6800 ext. 144 > > > CONFIDENTIALITY NOTICE: This electronic mail transmission and any accompanying data files is confidential and is intended exclusively for the individual or entity to which it is addressed. The communication may contain information that is proprietary, privileged or confidential or otherwise legally exempt from disclosure. If you are not the named addressee or you otherwise have received this message in error, you are not authorized to read, print, copy or disseminate this message or any part of it. If you have received this message in error, please notify the sender immediately by email and delete all copies of this message. Receipt by anyone other than the named addressee is not a waiver of any attorney-client work product or other applicable privilege. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission and any accompanying data files is confidential and is intended exclusively for the individual or entity to which it is addressed. The communication may contain information that is proprietary, privileged or confidential or otherwise legally exempt from disclosure. If you are not the named addressee or you otherwise have received this message in error, you are not authorized to read, print, copy or disseminate this message or any part of it. If you have received this message in error, please notify the sender immediately by email and delete all copies of this message. Receipt by anyone other than the named addressee is not a waiver of any attorney-client work product or other applicable privilege. From mpence at grhs.net Fri Nov 16 10:22:52 2018 From: mpence at grhs.net (Mike Pence) Date: Fri, 16 Nov 2018 16:22:52 +0000 Subject: [Histonet] Closed doors in Histology Message-ID: OK, How many of you keep your doors to your Histology Dept. closed all the time? We were told by a CIHQ inspector that Histology Dept must be under negative air flow ALL THE TIME. This was a new one for me. The standard is from the ASHRAE 170 table 7.1 This is just an FYI for you all that these are the kinds of things CMS is looking at now days. Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 mpence at grhs.net | www.greatrivermedical.org. www.Facebook.com/GreatRiverHealthSystems | www.Twitter/GreatRiverMed Information in this communication, including attachments, is confidential and intended only for the addressee(s). This communication may contain privileged, confidential, proprietary or trade secret information entitled to protection or exemption from disclosure under law. If you are not an intended recipient, please know that any use, distribution or copying of this communication, or any action taken based on the information in this communication, is unauthorized and may be unlawful. If you received this communication in error, please notify the sender and delete this communication from your device. From rjbuesa at yahoo.com Fri Nov 16 10:31:09 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 16 Nov 2018 16:31:09 +0000 (UTC) Subject: [Histonet] test In-Reply-To: References: <55DE4F3E-8101-43F5-84AF-0FEF18FA3545@gmail.com> Message-ID: <1477118477.1679190.1542385869228@mail.yahoo.com> If you add anything, and I mean ANITHING, to your message, the message will not be posted.I recommend you to post your problem again without the screenshot.Ren? On Friday, November 16, 2018 11:27 AM, "Campbell, Tasha M. via Histonet" wrote: Thanks.? So I got a copy of this email I sent.? But I sent my question twice and did not get the copy.? I put a screen shot in the message.? Could that mess it up? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 -----Original Message----- From: Eileen Akemi Allison [mailto:akemiat3377 at gmail.com] Sent: Friday, November 16, 2018 11:01 AM To: Campbell, Tasha M.; Histonet Subject: Re: [Histonet] test ** CAUTION: This email originated from outside of Frederick Memorial Hospital. DO NOT click on links or open attachments if you do not recognize the sender. ** I received it.? I have had the same problem lately.? I didn't get a copy of the email sent.? It would be nice to know that it was received. Think they changed their format. Akemi Allison > On Nov 16, 2018, at 7:25 AM, Campbell, Tasha M. via Histonet wrote: > > I keep trying to send an email for help with an issue I am having and its not going through. It says the email has been sent but I am not getting the email and no one has responded.? I am testing to see if this message goes through. > > > > > Tasha Campbell, B.S.,HTL(ASCP) > Frederick Gastroenterology Associates > 310 W. 9th St. > Frederick, MD 21701 > 301-695-6800 ext. 144 > > > CONFIDENTIALITY NOTICE: This electronic mail transmission and any accompanying data files is confidential and is intended exclusively for the individual or entity to which it is addressed. The communication may contain information that is proprietary, privileged or confidential or otherwise legally exempt from disclosure. If you are not the named addressee or you otherwise have received this message in error, you are not authorized to read, print, copy or disseminate this message or any part of it. If you have received this message in error, please notify the sender immediately by email and delete all copies of this message. Receipt by anyone other than the named addressee is not a waiver of any attorney-client work product or other applicable privilege. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission and any accompanying data files is confidential and is intended exclusively for the individual or entity to which it is addressed. The communication may contain information that is proprietary, privileged or confidential or otherwise legally exempt from disclosure. If you are not the named addressee or you otherwise have received this message in error, you are not authorized to read, print, copy or disseminate this message or any part of it. If you have received this message in error, please notify the sender immediately by email and delete all copies of this message. Receipt by anyone other than the named addressee is not a waiver of any attorney-client work product or other applicable privilege. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward at wakehealth.edu Fri Nov 16 10:33:09 2018 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Fri, 16 Nov 2018 16:33:09 +0000 Subject: [Histonet] Reproducibility in IHC validations Message-ID: My medical director and I are working on our procedure for reproducibility in our IHC validations and would like to know how others are handling this. How many slides do you run and over how many days/runs? If someone would share their procedure with us that would be wonderful. Thanks in advance for your help. Martha Ward Wake Forest Baptist Health From bakevictoria at gmail.com Fri Nov 16 10:54:54 2018 From: bakevictoria at gmail.com (Victoria Baker) Date: Fri, 16 Nov 2018 11:54:54 -0500 Subject: [Histonet] Closed doors in Histology In-Reply-To: References: Message-ID: Mike, Yes that is correct. Histology labs because of their toxic chemicals and less than pleasant odors need to keep the doors shut for proper ventilation. This doesn't always happen when there is a high volume of people going in and out. I like it to the requirement that there be a sink (clean sink) in every Histology lab too. We have two sinks, 1 for special stains and the other for tissue processor loading and formalin neutralization. Which sink do you think might be cleaner???? Vikki Baker On Fri, Nov 16, 2018, 11:35 AM Mike Pence via Histonet < histonet at lists.utsouthwestern.edu> wrote: > OK, How many of you keep your doors to your Histology Dept. closed all the > time? We were told by a CIHQ inspector that Histology Dept must be under > negative air flow ALL THE TIME. This was a new one for me. The standard is > from the ASHRAE 170 table 7.1 > This is just an FYI for you all that these are the kinds of things CMS is > looking at now days. > > Michael S. Pence | Supervisor of Laboratory Services > Great River Health Systems > 1221 S. Gear Ave. | West Burlington, IA 52655 > Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 > mpence at grhs.net | www.greatrivermedical.org< > http://www.greatrivermedical.org>. > www.Facebook.com/GreatRiverHealthSystems< > http://www.Facebook.com/GreatRiverHealthSystems> | > www.Twitter/GreatRiverMed > > > Information in this communication, including attachments, is confidential > and intended only for the addressee(s). This communication may contain > privileged, confidential, proprietary or trade secret information entitled > to protection or exemption from disclosure under law. If you are not an > intended recipient, please know that any use, distribution or copying of > this communication, or any action taken based on the information in this > communication, is unauthorized and may be unlawful. If you received this > communication in error, please notify the sender and delete this > communication from your device. > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Timothy.Morken at ucsf.edu Fri Nov 16 10:59:27 2018 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 16 Nov 2018 16:59:27 +0000 Subject: [Histonet] Closed doors in Histology In-Reply-To: References: Message-ID: That'll be tough for our histo lab - there are no doors. It is open to two hallways and adjacent clin lab. That said, we have so many hoods in there that we probably make the entire floor negative pressure! And there is no odor from the lab. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Mike Pence via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, November 16, 2018 8:23 AM To: histonet at lists.utsouthwestern.edu; histonet-bounces at lists.utsouthwestern.edu Subject: [Histonet] Closed doors in Histology OK, How many of you keep your doors to your Histology Dept. closed all the time? We were told by a CIHQ inspector that Histology Dept must be under negative air flow ALL THE TIME. This was a new one for me. The standard is from the ASHRAE 170 table 7.1 This is just an FYI for you all that these are the kinds of things CMS is looking at now days. Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 mpence at grhs.net | www.greatrivermedical.org. www.Facebook.com/GreatRiverHealthSystems | www.Twitter/GreatRiverMed Information in this communication, including attachments, is confidential and intended only for the addressee(s). This communication may contain privileged, confidential, proprietary or trade secret information entitled to protection or exemption from disclosure under law. If you are not an intended recipient, please know that any use, distribution or copying of this communication, or any action taken based on the information in this communication, is unauthorized and may be unlawful. If you received this communication in error, please notify the sender and delete this communication from your device. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmcampbe at fmh.org Fri Nov 16 10:59:28 2018 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Fri, 16 Nov 2018 16:59:28 +0000 Subject: [Histonet] Artifact Message-ID: Can anyone help me out with the artifact I am getting on my slides? It is on all of them, IHC, H&E, specials. It looks like formalin pigment, but I use 10%NBF so should I really be getting formalin pigment this much on my slides? I am a GI lab. I use microwave processing. Specimens are held 24-48 hours in formalin before being processed. It is on almost all my slides. Everyday. Its very frustrating trying to figure out the problem. If it is formalin pigment, how can I prevent it? My microwave process is a rinse in 95% alcohol, then 100% reagent alcohol for 8 min, Isopropyl for 9 minutes, and Paraffin for 14 min. I also use a slide etcher to label my slides and there are always dust particles in my water bath and getting on my slides. Could this be causing an issue? I tried to add a screenshot of the slides with the pigment but apparently adding anything like that to the message will not let the email go through. So I can privately email the images if someone could help me. Thanks! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 CONFIDENTIALITY NOTICE: This electronic mail transmission and any accompanying data files is confidential and is intended exclusively for the individual or entity to which it is addressed. The communication may contain information that is proprietary, privileged or confidential or otherwise legally exempt from disclosure. If you are not the named addressee or you otherwise have received this message in error, you are not authorized to read, print, copy or disseminate this message or any part of it. If you have received this message in error, please notify the sender immediately by email and delete all copies of this message. Receipt by anyone other than the named addressee is not a waiver of any attorney-client work product or other applicable privilege. From Valerie.Hannen at parrishmed.com Fri Nov 16 12:01:33 2018 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Fri, 16 Nov 2018 13:01:33 -0500 Subject: [Histonet] [External Sender] Re: Closed doors in Histology In-Reply-To: References: Message-ID: <450B7A81EDA0C54E97C53D60F00776C3243D9ECDB3@isexstore03> We have kept our doors closed at all times for years due to the negative pressure issue. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: Morken, Timothy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, November 16, 2018 11:59 AM To: Histonet Subject: [External Sender] Re: [Histonet] Closed doors in Histology WARNING: This message came from an external source. Please do not click links or open attachments if unexpected or unusual. That'll be tough for our histo lab - there are no doors. It is open to two hallways and adjacent clin lab. That said, we have so many hoods in there that we probably make the entire floor negative pressure! And there is no odor from the lab. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Mike Pence via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, November 16, 2018 8:23 AM To: histonet at lists.utsouthwestern.edu; histonet-bounces at lists.utsouthwestern.edu Subject: [Histonet] Closed doors in Histology OK, How many of you keep your doors to your Histology Dept. closed all the time? We were told by a CIHQ inspector that Histology Dept must be under negative air flow ALL THE TIME. This was a new one for me. The standard is from the ASHRAE 170 table 7.1 This is just an FYI for you all that these are the kinds of things CMS is looking at now days. Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 mpence at grhs.net | www.greatrivermedical.org. www.Facebook.com/GreatRiverHealthSystems | www.Twitter/GreatRiverMed Information in this communication, including attachments, is confidential and intended only for the addressee(s). This communication may contain privileged, confidential, proprietary or trade secret information entitled to protection or exemption from disclosure under law. If you are not an intended recipient, please know that any use, distribution or copying of this communication, or any action taken based on the information in this communication, is unauthorized and may be unlawful. If you received this communication in error, please notify the sender and delete this communication from your device. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From jvergara at micropathlabs.com Fri Nov 16 14:31:04 2018 From: jvergara at micropathlabs.com (Jacquilyn Vergara) Date: Fri, 16 Nov 2018 20:31:04 +0000 Subject: [Histonet] MSH2 and MSH6 validation Message-ID: We are having the same issue. I came across this thread. Did you find any tissue for this? Thanks! Jacquilyn R. Vergara MLS, HTL (ASCP)cm Email: jvergara at micropathlabs.com NOTICE OF CONFIDENTIALITY The information in this email, including attachments, may be confidential and/or privileged and may contain confidential health information. This email is intended to be reviewed only by the individual or organization named as addressee. If you have received this email in error please notify MicroPath Laboratories immediately by return message to the sender or to info at micropathlabs.com. Destroy all copies of this message and any attachments. Please note that any views or opinions presented in this email are solely those of the author and do not necessarily represent those of MicroPath Laboratories. Confidential health information is protected by state and federal law, including, but not limited to: The Health Insurance Portability and Accountability Act of 1996 and related regulations. From aj.taylor at blueyonder.co.uk Sat Nov 17 13:00:45 2018 From: aj.taylor at blueyonder.co.uk (taylor alan) Date: Sat, 17 Nov 2018 19:00:45 +0000 (GMT) Subject: [Histonet] Detached vinyl coverslips - any advice on reattachment? In-Reply-To: References: Message-ID: <341031961.1063473.1542481245606@mail2.virginmedia.com> Hi Gordon The issue you describe is a common one with tissue sections mounted with the old tape based cover slippers. We had one of these machines a few years ago, following years of hand mounting, it improved the throughput in the department quite successfully at the time. This machine was then replaced by a glass cover slipper. (which many of us wished we had bought in the first place) when we found several years down the line that when recovering archive slides for the pathologists to make comparisons with current diagnostic material it was found that many of these tape cover slipped sections had become detached from the glass slide, invariably the section was firmly attached to the convex underside of the plastic film. It was extremely difficult to re attach these sections to make them up to diagnostic quality. It meant in most cases that we had to pull the original blocks, do a re cut and present a freshly stained section, for comparison. Frustrating for all concerned, particularly the pathologist who had to wait for a replacement section. The tape was merely attached to the slide with xylene, no mounting medium was needed, the slide went through a roller device to secure the tape strip and then a blade came across, at a pre set measurement to cut and finish the mounted section. As you are keeping your slides for personal interest, you could try and remount them with a soak in xylene followed by dispensing a thin line of DPX onto the slide, gently placing the tape over this and applying a light weight to gently press the cover slip down to flatten it and then place the remounted slide, with the weight still in place, in the slide drying oven overnight, or a warm place for a day or two. If you have about 400 sections to remount this is going to take some time. As we are both based in the UK if you contact me at my above e mail address I will be very happy to discuss other alternatives with you. Either way you are going to have a busy time with the task in front of you. Kind Regards Alan Taylor BSc(Hons), FRMS. Exeter. UK. > > On 15 November 2018 at 20:56 Gordon Brown via Histonet wrote: > > I'm an amateur microscopist and along with a couple of fellow club members > we have acquired a fairly extensive collection of histopathology slides > (approx. 2500 slides) made in the early 2000s and used for seminar and > lecture purposes. We intend to use these to further our knowledge and > interest in histopathology and the majority of the slides are in superb > condition for this purpose and reasonably well documented. However, > approximately 300 to 400 of the slides are suffering from detachment of the > coverslip, these coverslips being made of what I assume to be vinyl, and we > are attempting to reattach them. Can anyone advise how this type of > coverslip was attached in the first instance? I'm assuming this was done > using an automatic coverslipper, but there's no easily visible trace of > mountant on either the slide or the coverslip so they may be self-adhesive > or use a heat activated adhesive. Each coverslip is 55.4mm x 24.1mm. > > Hope someone can help, if we know how they were attached in the first > instance this may give us some ideas for a strategy for reattachment. > > Regards > > Gordon > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gordon at 10db.co.uk Sat Nov 17 14:01:24 2018 From: gordon at 10db.co.uk (Gordon Brown) Date: Sat, 17 Nov 2018 20:01:24 -0000 Subject: [Histonet] Detached vinyl coverslips - any advice on reattachment? Message-ID: Many thanks to all who replied, looks like this could be a thankless and difficult task but we will persist in trying to reattach the coverslips that have the better quality sections. Meanwhile the vast remainder of the collection will keep us busy for quite some time! Gordon I'm an amateur microscopist and along with a couple of fellow club members we have acquired a fairly extensive collection of histopathology slides (approx. 2500 slides) made in the early 2000s and used for seminar and lecture purposes. We intend to use these to further our knowledge and interest in histopathology and the majority of the slides are in superb condition for this purpose and reasonably well documented. However, approximately 300 to 400 of the slides are suffering from detachment of the coverslip, these coverslips being made of what I assume to be vinyl, and we are attempting to reattach them. Can anyone advise how this type of coverslip was attached in the first instance? I'm assuming this was done using an automatic coverslipper, but there's no easily visible trace of mountant on either the slide or the coverslip so they may be self-adhesive or use a heat activated adhesive. Each coverslip is 55.4mm x 24.1mm. Hope someone can help, if we know how they were attached in the first instance this may give us some ideas for a strategy for reattachment. Regards Gordon From amber.m.villarreal at gmail.com Mon Nov 19 17:57:35 2018 From: amber.m.villarreal at gmail.com (Amber Villarreal) Date: Mon, 19 Nov 2018 15:57:35 -0800 Subject: [Histonet] Job openings at UC Davis, CA Message-ID: The UC Davis School of Veterinary Medicine Histology Department is expanding and has 2 positions currently open for Histology technicians in Davis, California. The listings can be found at the link below or by searching the Requisition #03022495 on the university site under staff jobs. https://www.employment.ucdavis.edu/applicants/jsp/shared/position/JobDetails_css.jsp?postingId=387521 Best Regards, Amber Villarreal, HTL(ASCP)CM Histology Lab Supervisor UC Davis Veterinary Medical Teaching Hospital From relia1 at earthlink.net Tue Nov 20 10:36:44 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 20 Nov 2018 11:36:44 -0500 Subject: [Histonet] Happy Thanksgiving! Message-ID: <000001d480ef$391a48f0$ab4edad0$@earthlink.net> Hello Histonetters, How are you? Happy Thanksgiving to you and yours! I wanted share with you one of the things that I am most Thankful for. You! Thank you for accepting my posts and reading my career bulletins. I know you aren?t looking for a job every time you open a post from me and I know that your in box is precious real estate. Histonetters, I hope that you enjoy the little tidbits that I share from time to time. And remember if you refer someone to me and I place them you will earn a referral bonus. And of course if you are looking for a new opportunity I am always here to help! I?m not putting any jobs in this post because I just want to say: Thank you!! Stay tuned for my NEXT post, I have a lot of exciting new positions. The great thing about these opportunities is that if you are ready to move right away my clients are too and if you want to pursue a position with a start date after the holidays that works as well!! If you are looking for a position RIGHT NOW! Please call me at 407-353-5070. And we can discuss my current openings. Have a Wonderful Thanksgiving!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From doolee at shands.ufl.edu Tue Nov 20 12:52:49 2018 From: doolee at shands.ufl.edu (Dooley, Elaine) Date: Tue, 20 Nov 2018 18:52:49 +0000 Subject: [Histonet] MAK-6 Message-ID: <408e4a21b44d4290847c8983075d6a27@shands.ufl.edu> Hi Histoneters, Does anyone know of a Vendor that carries anti-cytokeratin clone MAK-6? Elaine Dooley 352-265-0111 ext 72117 Shands Teaching Hospital Gainesville FL 32655 From madeathridge at pastnashville.com Tue Nov 20 14:36:45 2018 From: madeathridge at pastnashville.com (Maryann Deathridge) Date: Tue, 20 Nov 2018 20:36:45 +0000 Subject: [Histonet] Direct Immunofluorescence Message-ID: Happy Thanksgiving Histonetters ! My lab is wanting to possibly start doing DIF's in our laboratory. The lab currently performs IHC on the Dako Autolink 48 and Omnis. We have a cryostat and other laboratory instrumentation. I am looking for material and methods. Would appreciate any information. Thanks in advance! Maryann Deathridge, Lab Manager madeathridge at pastnashville.com From craigak12 at gmail.com Tue Nov 20 16:47:39 2018 From: craigak12 at gmail.com (J B) Date: Tue, 20 Nov 2018 15:47:39 -0700 Subject: [Histonet] AP LIS Message-ID: Does anyone have a great AP LIS system that they can recommend? Thank you, JB -- Have a great day! From Richard.Cartun at hhchealth.org Wed Nov 21 10:57:21 2018 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Wed, 21 Nov 2018 16:57:21 +0000 Subject: [Histonet] Question - EM Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EAC0D919D@HHCEXCHMB03.hhcsystem.org> How long can tissue remain in glutaraldehyde before EM testing is performed? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From greg.dobbin at gmail.com Wed Nov 21 14:06:12 2018 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Wed, 21 Nov 2018 16:06:12 -0400 Subject: [Histonet] Question - EM Message-ID: Hi Dr. Cartun, It has been many years since I worked in EM but I my recollection is that tissues could remain in 2% Glut indefinitely without detriment (for EM purposes). However, Osmium tetroxide had to have a limited exposure. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From tbraud at holyredeemer.com Wed Nov 21 15:28:16 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 21 Nov 2018 21:28:16 +0000 Subject: [Histonet] LIS recommendations Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF88F9B@HRHEX02-HOS.holyredeemer.local> LIS recommendations - I LOVE CoPath! Either version, but I am currently using the SunQuest version 6.1. Loads of flexibility. You can use it at it's most simplest, or add in all the bells and whistles. Easy to build and maintain, with well-established instrument interfaces and voice recognition programs. Not only am I the Supervisor, but I am also the CoPath Systems Manager. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From CDavis at che-east.org Fri Nov 23 10:09:25 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Fri, 23 Nov 2018 16:09:25 +0000 Subject: [Histonet] AP LIS In-Reply-To: References: Message-ID: We had CoPath and loved it, so easy to get stats, cases for controls tissue and nice looking reports Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From criley at dpspa.com Mon Nov 26 10:52:47 2018 From: criley at dpspa.com (Charles Riley) Date: Mon, 26 Nov 2018 11:52:47 -0500 Subject: [Histonet] Stainer Quotes Message-ID: Are there any vendors out there selling any of the following refurbished item? 1. Leica ST5010 or ST5020 stainer 2. Sakura DRS 2000 3. DiaPath Giotto 4. Shur/Stain 3030 -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From john at imebinc.com Mon Nov 26 12:23:28 2018 From: john at imebinc.com (John O'Brien) Date: Mon, 26 Nov 2018 10:23:28 -0800 Subject: [Histonet] Pathology Instruments Histonet Digest, Vol 180, Issue 21 Message-ID: <241e01d485b5$211d4c50$6357e4f0$@imebinc.com> IMEB INC sells all the Pathology equipment you have interest in, We prefer to sell the Sakura or Leica stainer because they are the most reliable we find after 30 years of pathology Service Regards, IMEB INC -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Monday, November 26, 2018 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 180, Issue 21 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Stainer Quotes (Charles Riley) ---------------------------------------------------------------------- Message: 1 Date: Mon, 26 Nov 2018 11:52:47 -0500 From: Charles Riley To: Histo List Subject: [Histonet] Stainer Quotes Message-ID: Content-Type: text/plain; charset="UTF-8" Are there any vendors out there selling any of the following refurbished item? 1. Leica ST5010 or ST5020 stainer 2. Sakura DRS 2000 3. DiaPath Giotto 4. Shur/Stain 3030 -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 180, Issue 21 ***************************************** From margaret at allprobiomed.com Mon Nov 26 12:34:48 2018 From: margaret at allprobiomed.com (margaret at allprobiomed.com) Date: Mon, 26 Nov 2018 10:34:48 -0800 Subject: [Histonet] Stainer Quotes In-Reply-To: References: Message-ID: <007701d485b6$b6765f20$23631d60$@allprobiomed.com> Hello Charles, We have a Sakura DRS 2000, also a Leica ST 5010 with the CV 5030 coverslipper workstation. All our instruments are completely refurbished and we include a one year warranty. Please email or call for a quote! Thanks, Margaret O?Donnell Margaret at allprobiomed.com 760-407-3006 Ext. 102 www.allprobiomedical.com -----Original Message----- From: Charles Riley [mailto:criley at dpspa.com] Sent: Monday, November 26, 2018 8:53 AM To: Histo List Subject: [Histonet] Stainer Quotes Are there any vendors out there selling any of the following refurbished item? 1. Leica ST5010 or ST5020 stainer 2. Sakura DRS 2000 3. DiaPath Giotto 4. Shur/Stain 3030 -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs --- This email has been checked for viruses by Avast antivirus software. https://www.avast.com/antivirus From Kelly.Pairan at nationwidechildrens.org Mon Nov 26 13:09:51 2018 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Mon, 26 Nov 2018 19:09:51 +0000 Subject: [Histonet] Glass Slide Marking Pens Message-ID: Good Afternoon Histoland, We are currently searching for marking pens to mark on glass slides. We used to buy the ones from Biohit but they no longer make those. Our pathologists like them in blue, red and green so the KP markers from Mercedes are out of the running. Are any of your pathologist using a colored pen to mark on slides that they love? We tried several different brands now and our pathologists have not liked them. Thanks, Kelly Kelly Pairan, HT (ASCP)CM, QIHC (ASCP) Histology Supervisor-Anatomic Pathology Department of Pathology and Laboratory Medicine Email: kelly.pairan at nationwidechildrens.org ph: 614-722-5414 fx: 614-722-3033 From tabbott at pennstatehealth.psu.edu Mon Nov 26 15:54:15 2018 From: tabbott at pennstatehealth.psu.edu (Abbott, Tanya) Date: Mon, 26 Nov 2018 21:54:15 +0000 Subject: [Histonet] Tissue discard Message-ID: <2164fe4a1d50425083835191a278e5be@EXCHSRVR12.mshmc.local> What sort of PPE does everyone use when "dumping" tissue? Interested mostly in if folks are using a N95 mask, respirator, PAPR? We pour the formalin off our tissues and bag the tissues to be taken away. Any input is appreciated! Tanya Tanya G. Abbott, HT (ASCP)CM, RT(CSMLS) Pathology Manager Penn State Health St. Joseph Reading, PA tabbott at pennstatehealth.psu.edu From plucas at biopath.org Wed Nov 28 07:58:57 2018 From: plucas at biopath.org (Paula) Date: Wed, 28 Nov 2018 05:58:57 -0800 Subject: [Histonet] sakura prisma stainer problem Message-ID: <001801d48722$82ddd180$88997480$@biopath.org> Hello..Tech support with Sakura is not open yet so I was hoping maybe someone can help me here. We have words on the computer screen for the Prisma stainer that shows something that we haven't seen before. The words, "Maintenance Program" is there. We shut it down and turned it on to get to our normal screen so we can start staining, but it still goes to this. If anyone can help us out..thank you in advance Paula From GauchV at amc.edu Wed Nov 28 09:01:05 2018 From: GauchV at amc.edu (Gauch, Vicki) Date: Wed, 28 Nov 2018 15:01:05 +0000 Subject: [Histonet] Derm Tissue for Immunofluorescence Message-ID: Hi everyone, We are currently looking at our length of retention for Derm cases for Immunofluorescence and we are wondering ... What is your process for retention of the frozen skin tissue? How long do you retain it? At the end of that time, do you process it into paraffin or discard it ? We are just trying to get a feel for how other labs are handling these tissues. Thanks so much, Vicki Gauch AMCH Dept. of Pathology ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From hegartyhelen at gmail.com Thu Nov 29 09:45:48 2018 From: hegartyhelen at gmail.com (hegartyhelen at gmail.com) Date: Thu, 29 Nov 2018 15:45:48 +0000 Subject: [Histonet] AB&T Message-ID: Hi there I would like to know a Histology laboratory user experience of Tracking systems. Specifically vantage versus AB&T? Any advice would be great. Thanks Helen Helen Hegarty From wdesalvo.cac at outlook.com Thu Nov 29 12:18:43 2018 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Thu, 29 Nov 2018 18:18:43 +0000 Subject: [Histonet] AB&T In-Reply-To: References: Message-ID: Helen, I have experience with both. Feel free to contact me directly with questions or we can arrange a call. More than happy to help William DeSalvo wdesalvo.cac at outlook. Com 480-622-1337 William DeSalvo ________________________________ From: Helen via Histonet Sent: Thursday, November 29, 2018 9:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] AB&T Hi there I would like to know a Histology laboratory user experience of Tracking systems. Specifically vantage versus AB&T? Any advice would be great. Thanks Helen Helen Hegarty _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff at uropartners.com Thu Nov 29 12:24:48 2018 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 29 Nov 2018 18:24:48 +0000 Subject: [Histonet] Our lab rocks. Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF11F3ABBE@COLOEXCH01.uropartners.local> Today's blog posting is celebrating the lab, but the link is not postable here. If you have any interest in reading, email me at lraff at uropartners.com and I will send the link. Thanks, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From jwwalker at rrmc.org Thu Nov 29 14:03:23 2018 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Thu, 29 Nov 2018 20:03:23 +0000 Subject: [Histonet] Cost per test averages In-Reply-To: <1047480436.965973.1542040070173@mail.yahoo.com> References: <48E053DDF6CE074DB6A7414BA05403F8ECF875AC@HRHEX02-HOS.holyredeemer.local> <1047480436.965973.1542040070173@mail.yahoo.com> Message-ID: This is very true, Rene but lab costs can vary widely due to group purchasing contracts and other negotiations that are slightly outside of the lab's control. When we performed this analysis, we look at consumable costs in addition to technologist's salary cost to figure out total cost per test/CPT code that we bill. For salary costs we utilized the following table with calculations (numbers are fictitious). The % per day is the total number of hours worked for 2 people divided by the hours/day. The % of salary is the FY salary divided by the %/day. The Salary/task is simply a division of the %salary by the FY volume for the task. Your consumable costs should be fairly easy to calculate based on what you pay for slides, cassettes, prefilled formalin, etc. We matched all of this up to our various CPT codes as a comparison to potential reimbursements. It may not be perfect but it gave us a pretty close guestimate and a tool to negotiate with vendors to help lower our costs against our reimbursement margin. Hours/Day %/Day FY Volume % of Salary Salary/Task Embed 2.5 15.6 11920 $ 15,625.00 $ 1.31 Microtomy 4.5 28.1 11920 $ 28,125.00 $ 2.36 Gross 3.0 18.8 11920 $ 18,750.00 $ 1.57 IHC/Special 2.0 12.5 2968 $ 12,500.00 $ 4.21 nonproductive time 4.0 25.0 $ 25,000.00 Total 16.0 100.0 FY Salary $ 100,000.00 Total for 2 people 16 Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Rene J Buesa via Histonet Sent: Monday, November 12, 2018 11:28 AM To: 'histonet at lists.utsouthwestern.edu' ; Terri Braud Subject: Re: [Histonet] Cost per test averages Terri: All you wrote is absolutely true BUT has nothing to do with the "negotiating" aspect Charlie us asking about.He asked about the price he wants to pay and that will depend on his purchase volume that could determine, if his' is large enough, may entice the seller to reduce his profit margin. Ren? On Monday, November 12, 2018, 9:57:37 AM EST, Terri Braud via Histonet > wrote: I find it very peculiar to be lectured by a Roche representative on including reagent management when calculating stain costs, considering Roche's yearlong ongoing issues with reagent supply problems and massive recalls. I've yet to have one month with no reagent supply problems from Roche/Ventana. Failed dispensers, multiple recalls, major delays in deliveries of supplies (the most recent was a TWO MONTH BACKORDER on a routinely run antibody) and the list goes on. Roche needs to get their own house in order before they come on a technical list-serve to lecture. Just saying... Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: Message: 2 Date: Sat, 10 Nov 2018 14:54:19 -0500 From: "Frazier, John" > To: Charles Riley > I?m a vendor that sells an H&M staining product, so I?m not going to attempt to give you what the average cost per of a H&E stained slide. What I will tell you is that when looking at cost per slide you need to calculate more than your consumables. Those are going to be your capital cost. You also need to calculate in your labor cost. Those are gonna be your operational dollars. The reason why I say that is that some strainers are more efficient than other stainer. Not just in the staining process itself but in the overall maintenance, reagent management and waste control cost. Remember when using labor dollars you want to calculate that using fully burdens dollars. Be complete in the way that you calculate your overall cost per slide John Frazier, MBA, MT(ASCP) Strategic Workflow Consulting Roche Diagnostics _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7C63642c620c254031b46708d648bbd1ba%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C636776368850257229&sdata=ogV6P6jJ7OXgFIhLXf2RN%2B5vNgCJmvFeXqk2gaAeR5Y%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7C63642c620c254031b46708d648bbd1ba%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C636776368850257229&sdata=ogV6P6jJ7OXgFIhLXf2RN%2B5vNgCJmvFeXqk2gaAeR5Y%3D&reserved=0 From sandra.cheasty at wisc.edu Thu Nov 29 14:04:08 2018 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Thu, 29 Nov 2018 20:04:08 +0000 Subject: [Histonet] Ortho-keratotic Mineralizing Odontogenic Cyst Message-ID: Hello all, Does anyone have any ideas on de-mineralizing an ortho-keratotic, mineralizing, odontogenic cyst? It is so hard the pathologist cannot cut it into small enough pieces to fit into a cassettes. (It's been in decal for days with no change.) I was thinking of taking one of the chunks and putting it in potassium hydroxide under the hood, and monitoring every half hour or so. Anyone have any other ideas? Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From jwwalker at rrmc.org Thu Nov 29 14:17:28 2018 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Thu, 29 Nov 2018 20:17:28 +0000 Subject: [Histonet] Cost per test averages In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F8ECF875AC@HRHEX02-HOS.holyredeemer.local> <1047480436.965973.1542040070173@mail.yahoo.com> Message-ID: Well, the formatting of the table went awry. Contact me personal for it. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewalker at rrmc.org, www.rrmc.org -----Original Message----- From: Joe W. Walker, Jr. via Histonet Sent: Thursday, November 29, 2018 3:03 PM To: Rene J Buesa ; Terri Braud Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Cost per test averages [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. This is very true, Rene but lab costs can vary widely due to group purchasing contracts and other negotiations that are slightly outside of the lab's control. When we performed this analysis, we look at consumable costs in addition to technologist's salary cost to figure out total cost per test/CPT code that we bill. For salary costs we utilized the following table with calculations (numbers are fictitious). The % per day is the total number of hours worked for 2 people divided by the hours/day. The % of salary is the FY salary divided by the %/day. The Salary/task is simply a division of the %salary by the FY volume for the task. Your consumable costs should be fairly easy to calculate based on what you pay for slides, cassettes, prefilled formalin, etc. We matched all of this up to our various CPT codes as a comparison to potential reimbursements. It may not be perfect but it gave us a pretty close guestimate and a tool to negotiate with vendors to help lower our costs against our reimbursement margin. Hours/Day %/Day FY Volume % of Salary Salary/Task Embed 2.5 15.6 11920 $ 15,625.00 $ 1.31 Microtomy 4.5 28.1 11920 $ 28,125.00 $ 2.36 Gross 3.0 18.8 11920 $ 18,750.00 $ 1.57 IHC/Special 2.0 12.5 2968 $ 12,500.00 $ 4.21 nonproductive time 4.0 25.0 $ 25,000.00 Total 16.0 100.0 FY Salary $ 100,000.00 Total for 2 people 16 Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager joewalker at rrmc.org, https://na01.safelinks.protection.outlook.com/?url=www.rrmc.org&data=02%7C01%7Cjwwalker%40rrmc.org%7C76e6f15dbc9b4708e7fe08d65635ee26%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C636791186961127622&sdata=DTOV84zW9bAhJ5G8vHX40ww3dJbiyWF68W6wLxUxMZY%3D&reserved=0 -----Original Message----- From: Rene J Buesa via Histonet Sent: Monday, November 12, 2018 11:28 AM To: 'histonet at lists.utsouthwestern.edu' ; Terri Braud Subject: Re: [Histonet] Cost per test averages Terri: All you wrote is absolutely true BUT has nothing to do with the "negotiating" aspect Charlie us asking about.He asked about the price he wants to pay and that will depend on his purchase volume that could determine, if his' is large enough, may entice the seller to reduce his profit margin. Ren? On Monday, November 12, 2018, 9:57:37 AM EST, Terri Braud via Histonet > wrote: I find it very peculiar to be lectured by a Roche representative on including reagent management when calculating stain costs, considering Roche's yearlong ongoing issues with reagent supply problems and massive recalls. I've yet to have one month with no reagent supply problems from Roche/Ventana. Failed dispensers, multiple recalls, major delays in deliveries of supplies (the most recent was a TWO MONTH BACKORDER on a routinely run antibody) and the list goes on. Roche needs to get their own house in order before they come on a technical list-serve to lecture. Just saying... Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: Message: 2 Date: Sat, 10 Nov 2018 14:54:19 -0500 From: "Frazier, John" > To: Charles Riley > I?m a vendor that sells an H&M staining product, so I?m not going to attempt to give you what the average cost per of a H&E stained slide. What I will tell you is that when looking at cost per slide you need to calculate more than your consumables. Those are going to be your capital cost. You also need to calculate in your labor cost. Those are gonna be your operational dollars. The reason why I say that is that some strainers are more efficient than other stainer. Not just in the staining process itself but in the overall maintenance, reagent management and waste control cost. Remember when using labor dollars you want to calculate that using fully burdens dollars. Be complete in the way that you calculate your overall cost per slide John Frazier, MBA, MT(ASCP) Strategic Workflow Consulting Roche Diagnostics _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7C76e6f15dbc9b4708e7fe08d65635ee26%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C636791186961127622&sdata=7VkQRk629jY2WalmVAlVYWwQFBaCiVyUqs4PTjpbpKo%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7C76e6f15dbc9b4708e7fe08d65635ee26%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C636791186961127622&sdata=7VkQRk629jY2WalmVAlVYWwQFBaCiVyUqs4PTjpbpKo%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7C76e6f15dbc9b4708e7fe08d65635ee26%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C636791186961127622&sdata=7VkQRk629jY2WalmVAlVYWwQFBaCiVyUqs4PTjpbpKo%3D&reserved=0