From shultz11 at cox.net Tue May 1 12:07:48 2018 From: shultz11 at cox.net (shultz11) Date: Tue, 01 May 2018 12:07:48 -0500 Subject: [Histonet] Leica Message-ID: <20180501170752.BTVN7322.eastrmfepo202.cox.net@eastrmimpo209.cox.net> Leica equipment is now being made in China not Germany. I wish I would have known this before purchasing equipment. Kendra ShultzSent from my Verizon, Samsung Galaxy smartphone From cforster at umn.edu Tue May 1 12:24:10 2018 From: cforster at umn.edu (Colleen Forster) Date: Tue, 1 May 2018 12:24:10 -0500 Subject: [Histonet] Leica In-Reply-To: <20180501170752.BTVN7322.eastrmfepo202.cox.net@eastrmimpo209.cox.net> References: <20180501170752.BTVN7322.eastrmfepo202.cox.net@eastrmimpo209.cox.net> Message-ID: ? On Tue, May 1, 2018 at 12:07 PM, shultz11 via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > Leica equipment is now being made in China not Germany. I wish I would > have known this before purchasing equipment. > Kendra ShultzSent from my Verizon, Samsung Galaxy smartphone > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jshelley at sbpdiscovery.org Tue May 1 12:45:18 2018 From: jshelley at sbpdiscovery.org (John Shelley) Date: Tue, 1 May 2018 17:45:18 +0000 Subject: [Histonet] Florida Society for Histotechnology Message-ID: Hi All, If you are interested in getting some CEU's, we have a great line-up of workshops. Click on link below for program details: http://www.fshgroup.org/wp-content/uploads/2018/03/FSH_Program_May_17_20_2018-rev.pdf The meeting will be taking place at the Renaissance Tampa International Plaza Hotel on May 18- 20, 2018. If you have any questions contact John Shelley at jshelley at fshgroup.org. Look forward to seeing you at the meeting! Kind Regards! John J Shelley 2014-2018 FSH President From jaylundgren at gmail.com Tue May 1 13:04:12 2018 From: jaylundgren at gmail.com (Jay Lundgren) Date: Tue, 1 May 2018 13:04:12 -0500 Subject: [Histonet] Leica In-Reply-To: References: <20180501170752.BTVN7322.eastrmfepo202.cox.net@eastrmimpo209.cox.net> Message-ID: Sad, the last Leica microtome I bought was packed in German newspapers. So much for the hundreds of years of tradition of precision German instrumentation and optics. On Tue, May 1, 2018 at 12:24 PM, Colleen Forster via Histonet < histonet at lists.utsouthwestern.edu> wrote: > ? > > On Tue, May 1, 2018 at 12:07 PM, shultz11 via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > > > Leica equipment is now being made in China not Germany. I wish I would > > have known this before purchasing equipment. > > Kendra ShultzSent from my Verizon, Samsung Galaxy smartphone > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From vavalos at dermatologyaz.com Wed May 2 11:47:04 2018 From: vavalos at dermatologyaz.com (Vanessa Avalos) Date: Wed, 2 May 2018 16:47:04 +0000 Subject: [Histonet] Folded Sections Message-ID: >From one day to the next I am getting folded sections after staining. There does not seem to be an issue when sectioning. I see the folding once the slide is stained. I only do skin, any ideas what is going on? No changes in procedure or reagents from last week to this week. V. Avalos ADS HISTO From saundra.ellis at floridawomancare.com Wed May 2 13:03:26 2018 From: saundra.ellis at floridawomancare.com (Saundra Ellis) Date: Wed, 2 May 2018 18:03:26 +0000 Subject: [Histonet] Folded Sections In-Reply-To: References: Message-ID: You may have a tissue adhesions problem. Your tissue may not be dry completely before going to the stainer said the sections are lifting and folded in the reagent bath. Saundra Ellis Histology Supervisor Florida Woman Care Laboratory Cell (360) 513-9665 (best contact) FAX (813) 433-5542 saundra.ellis at floridawomancare.com ________________________________ From: Vanessa Avalos via Histonet Sent: Wednesday, May 2, 2018 12:47:04 PM To: HISTONET LISTS Subject: [Histonet] Folded Sections >From one day to the next I am getting folded sections after staining. There does not seem to be an issue when sectioning. I see the folding once the slide is stained. I only do skin, any ideas what is going on? No changes in procedure or reagents from last week to this week. V. Avalos ADS HISTO _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff at uropartners.com Mon May 7 07:57:00 2018 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 7 May 2018 12:57:00 +0000 Subject: [Histonet] Pathology/Pathologist Blog Post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF11A3D52B@COLOEXCH01.uropartners.local> Greetings to all, as spring finally descends on Chicago. This Month's Lab Blog Post-It's All in the Call Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From relia1 at earthlink.net Mon May 7 11:07:32 2018 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 7 May 2018 12:07:32 -0400 Subject: [Histonet] Exciting Opportunity for a Histology Coordinator A RELIA EXCLUSIVE!!! Message-ID: <000001d3e61d$81e22d40$85a687c0$@earthlink.net> Hi Histonetters, How are you? I hope this is the start to a great week for you! I have an exciting opportunity to share and if you aren't interested maybe you know someone who might be! I have been engaged on an exclusive search by one of my best clients located in the Dallas/Fort Worth area that is in need of a: Histology Quality Assurance Coordinator. This is a full time permanent position in Texas in the DFW area. My client is looking for an ASCP certified HT/HTL with strong routine histology, IHC, QA and training experience and ability. They are offering an excellent compensation package. The help I need from you Histonetters is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them or pass their contact information to me? *remember if I place someone you refer to me you will earn a referral bonus! If you are interested in this position please contact me ASAP on my cell/text 407-353-5070 or toll free at 866-607-3542 or via email at relia1 at earthlink.net If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won't release it to anyone without your permission. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From CarenAnn.Echague at DignityHealth.org Tue May 8 10:34:17 2018 From: CarenAnn.Echague at DignityHealth.org (Echague, Caren Ann - MGH) Date: Tue, 8 May 2018 15:34:17 +0000 Subject: [Histonet] IHC validation In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F8C1B89136@HRHEX02-HOS.holyredeemer.local> Message-ID: Where do you purchase your Tisssue Micro arrays? I know they are quite expensive. We are currently running validations and using only a handful of positive cases. Most of them don't have ten positive and negative cases but as long as you can get a statement from the medical director, this should be CAP compliant. Cae Aguilar, HTL (ASCP) Histology Supervisor 7079802801 -----Original Message----- From: Allan Wang [mailto:allanw at biomax.us] Sent: Thursday, March 22, 2018 11:02 AM Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC validation How are labs validating for rarer biomarkers like ALK, ROS1, or MMR loss? Following the guideline of 10 positive cases may be difficult. I've seen other companies with control slides just with a few engineered cell lines as positive and negative controls. Is that enough for validation alone? We are creating TMAs for validation of ALK and MMR loss (MLH1, PMS2, MSH6, MSH2) so I am interested in knowing how many cases are desired. Allan Allan Wang Lab Manager US Biomax On Tue, Mar 20, 2018 at 1:54 PM, Terri Braud via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Just another note: You can order unstained tissue microarrays with > the prerequisite number of cases, both positive and negative, and > stain your validation all on one slide. I've done this for years and > for 3 different validations of entire IHC platform changes, ranging > from 40 to over 100 antibodies each time. Saves time and money. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Message: 2 > Date: Fri, 16 Mar 2018 06:54:30 -0700 > From: "Paula" > Subject: [Histonet] Antibody Validation CLIA > > Hello, > We've been discussing about the quantity of slides to run as a > validation for IHC antibodies. We are governed by CLIA, and we would > like to know if there is a set number of slides to run for a > particular antibody we would like to bring in-house for Validation. I think CAP requires 20 slides..? > And so we are asking if there is a requirement with CLIA to run a > certain number of slides, or is it up to us (the laboratory director) > to decide how many slides to run for Validation/Verification. > Thank you in advance > Paula > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From criley at dpspa.com Tue May 8 13:05:52 2018 From: criley at dpspa.com (Charles Riley) Date: Tue, 8 May 2018 14:05:52 -0400 Subject: [Histonet] Special stain questions Message-ID: What special stains can be done on normal uvular tissue and umbilical cord? Trying to put together a small colorful histo project to get people educated about our field -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From jaylundgren at gmail.com Tue May 8 13:57:20 2018 From: jaylundgren at gmail.com (Jay Lundgren) Date: Tue, 8 May 2018 13:57:20 -0500 Subject: [Histonet] Special stain questions In-Reply-To: References: Message-ID: Any of them? If you mean what stain looks pretty, I would say Trichrome on umbilical, and uvula, hmm, I dunno. On Tue, May 8, 2018 at 1:05 PM, Charles Riley via Histonet < histonet at lists.utsouthwestern.edu> wrote: > What special stains can be done on normal uvular tissue and umbilical cord? > > Trying to put together a small colorful histo project to get people > educated about our field > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From modz9636 at gmail.com Tue May 8 16:50:42 2018 From: modz9636 at gmail.com (M.O.) Date: Tue, 8 May 2018 14:50:42 -0700 Subject: [Histonet] Paraffin infiltration issue, will this impact IHC? Message-ID: Hello Histonet, I have some samples that had an issue with the paraffin infiltration, but I am able to get okay sections. Will this impact IHC? Thank you, Merissa From greg.dobbin at gmail.com Wed May 9 13:26:57 2018 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Wed, 9 May 2018 15:26:57 -0300 Subject: [Histonet] Paraffin infiltration issue, will this impact IHC? Message-ID: Hi Merissa, As far as I am aware, insufficient paraffin infiltration would only affect sectioning. The epitopes that we we are attempting to stain with IHC are affected by pre-analytic factors such as fixation, cold ischemic time and perhaps heat (too much) but plus or minus wax should not be an issue if you are able to obtain good sections. Have you stained these poorly infiltrated specimens with H&E? Are you certain you have good sections with all tissue components represented? That would be my main concern. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From jobposting2600 at gmail.com Wed May 9 16:26:22 2018 From: jobposting2600 at gmail.com (Office Manager) Date: Wed, 9 May 2018 14:26:22 -0700 Subject: [Histonet] Histotech Position - Chandler AZ Message-ID: *Essential functions and responsibilities:* - Ensures compliance with all local, federal, CLIA, regulations. - Performs routine and non-routine activities involved in the preparation of slides for microscopic evaluation by pathologist(s), according to policies and procedures. - Responsible for embedding of surgical pathology specimens. - Responsible for the sectioning and microtomy of surgical pathology specimens. - Responsible for the routine staining, histochemical staining, and immunohistochemical staining of surgical pathology specimens for microscope evaluation and analysis. - Performs routine maintenance and cleaning of equipment and troubleshoots minor equipment failures. Documents remedial actions such as repairs or repeated tests. - Adheres to laboratory?s quality control policies and documents all quality control activities. - Maintains diagnostic viability of all specimens and ensures correct patient labeling. - Unpacks orders received; dates and stocks orders, in accordance with established policies and procedures. - Maintain a clean and safe prep work area in accordance with all laboratory and safety SOP?s. - Prepares and labels necessary stains and reagents in accordance with departmental procedures, policies and standards. - Reviews and performs QC on slides before taking the slides to the pathologist. - Maintenance of accurate work records and logs (i.e., Discrepancy Logs, Maintenance Logs, Quality Control Logs etc.) - Demonstrates a commitment to service, organizational values, and professionalism through appropriate conduct and demeanor at all times. - Works collaboratively and supports efforts of team members. - Protects patient information by adhering to professional standards including the Health Information Portability and Accountability Act (HIPAA). *Knowledge and Skill:* - Must have 2+ years laboratory experience. - HT Certified preferred. - Must be precise when performing technical tasks. - Effective interpersonal skills, both in person and on the telephone. - High accuracy in work and attention to detail. *Physical demands:* - Must have manual dexterity and motor coordination. - May involve sitting, standing, walking for long periods of time and/ or sitting at the microtome for long periods of time. - Exposure to chemicals and fumes. *Benefit Package:* - Health, Dental and Vision Insurance, 401K, Paid time off, Sick time and Holiday Pay. *Required education:* - Associate?s Degree or higher *Required experience and certification:* - Laboratory: 2 years - HT Certification ASCP preferred. East Valley Gastroenterology & Hepatology Associates is an Equal Opportunity Employer. This company does not and will not discriminate in employment and personnel practices on the basis of race, sex, age, handicap, religion, national origin or any other basis prohibited by applicable law. Hiring, transferring and promotion practices are performed without regard to the above listed items. Job Type: Full-time Reports to: Laboratory Manager Schedule: 40 Hours per Week Salary: DOE FLSA Status: Hourly; Non-Exempt To apply please reply to jobposting2600 at gmail.com or fax resume to (480) 786-6996, Attn:Isaac Malagon From plucas at biopath.org Thu May 10 09:13:10 2018 From: plucas at biopath.org (Paula) Date: Thu, 10 May 2018 07:13:10 -0700 Subject: [Histonet] IHC and Histogel Message-ID: <00b401d3e869$0802d5d0$18088170$@biopath.org> Hello good morning everyone, Can someone reply who knows if Histogel causes any interference with IHC staining? Thank you in advance, Paula Bio-Path Med Group Fountain Valley, CA From dunatrsd at sbcglobal.net Thu May 10 09:19:55 2018 From: dunatrsd at sbcglobal.net (dusko trajkovic) Date: Thu, 10 May 2018 14:19:55 +0000 (UTC) Subject: [Histonet] IHC and Histogel In-Reply-To: <00b401d3e869$0802d5d0$18088170$@biopath.org> References: <00b401d3e869$0802d5d0$18088170$@biopath.org> Message-ID: <421325481.1995533.1525961995270@mail.yahoo.com> We have used Histogel for years on cell pellets, and have not come across any IHC issues.Dusko On Thursday, May 10, 2018 7:13 AM, Paula via Histonet wrote: Hello good morning everyone, Can someone reply who knows if Histogel causes any interference with IHC staining? Thank you in advance, Paula Bio-Path Med Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jshelley at sbpdiscovery.org Thu May 10 09:22:07 2018 From: jshelley at sbpdiscovery.org (John Shelley) Date: Thu, 10 May 2018 14:22:07 +0000 Subject: [Histonet] IHC and Histogel In-Reply-To: <421325481.1995533.1525961995270@mail.yahoo.com> References: <00b401d3e869$0802d5d0$18088170$@biopath.org> <421325481.1995533.1525961995270@mail.yahoo.com> Message-ID: I agree with Dusko, I have used histogel the same way and have also used it for FISH and ISH without any issues. Kind Regards! ? John J Shelley Histology Core Manager Sanford Burnham Prebys Medical Discovery Institute at Lake Nona 6400 Sanger Road??????????????????????????????? Orlando, FL 32827??????????????????????????????????? Tel: (407) 745-2000 Ext.2517 Lab: (407) 745-2119 Fax: (407) 745-2001 ****email:? jshelley at SBPdiscovery.org ??****Please note email change -----Original Message----- From: dusko trajkovic via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, May 10, 2018 10:20 AM To: Paula; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC and Histogel We have used Histogel for years on cell pellets, and have not come across any IHC issues.Dusko On Thursday, May 10, 2018 7:13 AM, Paula via Histonet wrote: Hello good morning everyone, Can someone reply who knows if Histogel causes any interference with IHC staining? Thank you in advance, Paula Bio-Path Med Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjshelley%40sbpdiscovery.org%7C217545f9b96c4e33e47a08d5b6812003%7C0b162723004547deb0699f1a7aa955a1%7C0%7C0%7C636615588083420177&sdata=LTCmir6KeMPW%2FGwPz5fdtjioshiu0OhqLYjhh6zJSPg%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjshelley%40sbpdiscovery.org%7C217545f9b96c4e33e47a08d5b6812003%7C0b162723004547deb0699f1a7aa955a1%7C0%7C0%7C636615588083420177&sdata=LTCmir6KeMPW%2FGwPz5fdtjioshiu0OhqLYjhh6zJSPg%3D&reserved=0 From criley at dpspa.com Thu May 10 09:50:52 2018 From: criley at dpspa.com (Charles Riley) Date: Thu, 10 May 2018 10:50:52 -0400 Subject: [Histonet] Shandon Veristain Gemini Autostainer Message-ID: Has anyone had any issues with this tissue stainer dropping or crushing racks? We use it to run our gyn and non gyn pap/thin prep slides. Recently it has been tipping over the slide racks in the middle of the procedure and breaking the slides -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From histo at pathlab.us Thu May 10 10:25:24 2018 From: histo at pathlab.us (Histology) Date: Thu, 10 May 2018 15:25:24 +0000 Subject: [Histonet] benchtop fume hoods Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD8206EB91B3@vdc01.domain.local> Hi all, Can anyone share what type of benchtop fume hoods they are using in the lab? This would be for manual coverslipping and grossing. Thanks in advance! From mtoole at dcol.net Thu May 10 10:43:41 2018 From: mtoole at dcol.net (Mike Toole) Date: Thu, 10 May 2018 10:43:41 -0500 Subject: [Histonet] Shandon Veristain Gemini Autostainer In-Reply-To: References: Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB358CFE1F483@mail> Charles, The issue is most likely the handle for the rack, The rack has a moveable handle. Make sure the handle is securely in the upright position. It has pins and holes that should line up and click into place. If it is even slightly at an angle or not securely snapped into an upright position it may bump into the side of the stain pots (and really cause a mess). Mike -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, May 10, 2018 9:51 AM To: Histo List Subject: [EXTERNAL] [Histonet] Shandon Veristain Gemini Autostainer Has anyone had any issues with this tissue stainer dropping or crushing racks? We use it to run our gyn and non gyn pap/thin prep slides. Recently it has been tipping over the slide racks in the middle of the procedure and breaking the slides -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From allanw at biomax.us Thu May 10 11:38:28 2018 From: allanw at biomax.us (Allan Wang) Date: Thu, 10 May 2018 12:38:28 -0400 Subject: [Histonet] benchtop fume hoods In-Reply-To: <09CFA3F99D5B2B42B88CDFB2FC4CFD8206EB91B3@vdc01.domain.local> References: <09CFA3F99D5B2B42B88CDFB2FC4CFD8206EB91B3@vdc01.domain.local> Message-ID: I'd like to know as well. Thanks, Allan On Thu, May 10, 2018 at 11:25 AM, Histology via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all, > > Can anyone share what type of benchtop fume hoods they are using in the > lab? This would be for manual coverslipping and grossing. > > Thanks in advance! > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From paula at excaliburpathology.com Thu May 10 12:08:05 2018 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Thu, 10 May 2018 17:08:05 +0000 (UTC) Subject: [Histonet] benchtop fume hoods In-Reply-To: References: <09CFA3F99D5B2B42B88CDFB2FC4CFD8206EB91B3@vdc01.domain.local> Message-ID: <1188507992.2111931.1525972085826@mail.yahoo.com> I use a Labconco.?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Allan Wang via Histonet To: Cc: "histonet at lists.utsouthwestern.edu" Sent: Thursday, May 10, 2018 12:02 PM Subject: Re: [Histonet] benchtop fume hoods I'd like to know as well. Thanks, Allan On Thu, May 10, 2018 at 11:25 AM, Histology via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all, > > Can anyone share what type of benchtop fume hoods they are using in the > lab?? This would be for manual coverslipping and grossing. > > Thanks in advance! > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hackerlab at aol.com Thu May 10 14:18:49 2018 From: hackerlab at aol.com (James Mullen) Date: Thu, 10 May 2018 15:18:49 -0400 Subject: [Histonet] benchtop fume hoods In-Reply-To: Message-ID: <1634b7ddc55-17aa-1eb2@webjas-vac215.srv.aolmail.net> Hi Allan, Not trying to advertise, but, we have been making a Downdraft Fume Extraction Workstation for 40+ years. Lots of folks in Histoland have had one at one time or another. Perfect for coverslipping and/or grossing. If you are interested I would be glad to send you some info. Jim Best Regards, James Mullen, Jr., P.E. Vice President Hacker Instruments & Industries, Inc. 1132 Kincaid Bridge Road PO Box 1176 Winnsboro, SC 29180 www.hackerinstruments.com (803) 712-6100 -----Original Message----- From: Allan Wang via Histonet Cc: histonet Sent: Thu, May 10, 2018 1:01 pm Subject: Re: [Histonet] benchtop fume hoods I'd like to know as well. Thanks, Allan On Thu, May 10, 2018 at 11:25 AM, Histology via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all, > > Can anyone share what type of benchtop fume hoods they are using in the > lab? This would be for manual coverslipping and grossing. > > Thanks in advance! > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From badams at acadianagastro.com Thu May 10 17:59:44 2018 From: badams at acadianagastro.com (Brent Adams) Date: Thu, 10 May 2018 22:59:44 +0000 Subject: [Histonet] Histonet Digest, Vol 174, Issue 7- Benchtop fume hood. (Alan Wagg) In-Reply-To: References: Message-ID: Hey Alan, I use a Airfiltronix Ductless Bench Top Hood and it meets all my needs. I gross about 7500-8000 GI biopsies per year and do any hand cover slipping under this hood (which is rare if I can help it). The unit is made of clear acrylic, is see thru and is framed out in light weight aluminum. It has a clear Velcroed thick mil roll up sash which is very easy to work with. The system has two blower units on top. Both units have 2 inch carbon filters that I purchase from Mercedes Medical and replace twice a year. I did add a grated plastic secondary containment box which I bought through Grainger to place the unit on so that I could keep my formalin cube under the hood and not worry about spillage. This also elevated the work surface off the counter by 8-10 inches so you are not leaning over the work and therefore saving your back. I had this unit tested by an outside agency along with the whole lab before we started operation and then again after we started operation. Our PEL numbers for Formalin, Alcohol and Xylene were barley negligible. I have used the badge system every year since the original testing with the same results. This to me is the key to selecting any hood, does it keep the PEL numbers within exposure limits. The purchase price is around $2800.00 + filters @ $180 ea. Hope this helps and good luck. Brent Adams ? BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Thursday, May 10, 2018 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 174, Issue 7 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Paraffin infiltration issue, will this impact IHC? (Greg Dobbin) 2. Histotech Position - Chandler AZ (Office Manager) 3. IHC and Histogel (Paula) 4. Re: IHC and Histogel (dusko trajkovic) 5. Re: IHC and Histogel (John Shelley) 6. Shandon Veristain Gemini Autostainer (Charles Riley) 7. benchtop fume hoods (Histology) 8. Re: Shandon Veristain Gemini Autostainer (Mike Toole) 9. Re: benchtop fume hoods (Allan Wang) ---------------------------------------------------------------------- Message: 1 Date: Wed, 9 May 2018 15:26:57 -0300 From: Greg Dobbin To: modz9636 at gmail.com, histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin infiltration issue, will this impact IHC? Message-ID: Content-Type: text/plain; charset="UTF-8" Hi Merissa, As far as I am aware, insufficient paraffin infiltration would only affect sectioning. The epitopes that we we are attempting to stain with IHC are affected by pre-analytic factors such as fixation, cold ischemic time and perhaps heat (too much) but plus or minus wax should not be an issue if you are able to obtain good sections. Have you stained these poorly infiltrated specimens with H&E? Are you certain you have good sections with all tissue components represented? That would be my main concern. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* ------------------------------ Message: 2 Date: Wed, 9 May 2018 14:26:22 -0700 From: Office Manager To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Histotech Position - Chandler AZ Message-ID: Content-Type: text/plain; charset="UTF-8" *Essential functions and responsibilities:* - Ensures compliance with all local, federal, CLIA, regulations. - Performs routine and non-routine activities involved in the preparation of slides for microscopic evaluation by pathologist(s), according to policies and procedures. - Responsible for embedding of surgical pathology specimens. - Responsible for the sectioning and microtomy of surgical pathology specimens. - Responsible for the routine staining, histochemical staining, and immunohistochemical staining of surgical pathology specimens for microscope evaluation and analysis. - Performs routine maintenance and cleaning of equipment and troubleshoots minor equipment failures. Documents remedial actions such as repairs or repeated tests. - Adheres to laboratory?s quality control policies and documents all quality control activities. - Maintains diagnostic viability of all specimens and ensures correct patient labeling. - Unpacks orders received; dates and stocks orders, in accordance with established policies and procedures. - Maintain a clean and safe prep work area in accordance with all laboratory and safety SOP?s. - Prepares and labels necessary stains and reagents in accordance with departmental procedures, policies and standards. - Reviews and performs QC on slides before taking the slides to the pathologist. - Maintenance of accurate work records and logs (i.e., Discrepancy Logs, Maintenance Logs, Quality Control Logs etc.) - Demonstrates a commitment to service, organizational values, and professionalism through appropriate conduct and demeanor at all times. - Works collaboratively and supports efforts of team members. - Protects patient information by adhering to professional standards including the Health Information Portability and Accountability Act (HIPAA). *Knowledge and Skill:* - Must have 2+ years laboratory experience. - HT Certified preferred. - Must be precise when performing technical tasks. - Effective interpersonal skills, both in person and on the telephone. - High accuracy in work and attention to detail. *Physical demands:* - Must have manual dexterity and motor coordination. - May involve sitting, standing, walking for long periods of time and/ or sitting at the microtome for long periods of time. - Exposure to chemicals and fumes. *Benefit Package:* - Health, Dental and Vision Insurance, 401K, Paid time off, Sick time and Holiday Pay. *Required education:* - Associate?s Degree or higher *Required experience and certification:* - Laboratory: 2 years - HT Certification ASCP preferred. East Valley Gastroenterology & Hepatology Associates is an Equal Opportunity Employer. This company does not and will not discriminate in employment and personnel practices on the basis of race, sex, age, handicap, religion, national origin or any other basis prohibited by applicable law. Hiring, transferring and promotion practices are performed without regard to the above listed items. Job Type: Full-time Reports to: Laboratory Manager Schedule: 40 Hours per Week Salary: DOE FLSA Status: Hourly; Non-Exempt To apply please reply to jobposting2600 at gmail.com or fax resume to (480) 786-6996, Attn:Isaac Malagon ------------------------------ Message: 3 Date: Thu, 10 May 2018 07:13:10 -0700 From: "Paula" To: Subject: [Histonet] IHC and Histogel Message-ID: <00b401d3e869$0802d5d0$18088170$@biopath.org> Content-Type: text/plain; charset="us-ascii" Hello good morning everyone, Can someone reply who knows if Histogel causes any interference with IHC staining? Thank you in advance, Paula Bio-Path Med Group Fountain Valley, CA ------------------------------ Message: 4 Date: Thu, 10 May 2018 14:19:55 +0000 (UTC) From: dusko trajkovic To: Paula , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] IHC and Histogel Message-ID: <421325481.1995533.1525961995270 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 We have used Histogel for years on cell pellets, and have not come across any IHC issues.Dusko On Thursday, May 10, 2018 7:13 AM, Paula via Histonet wrote: Hello good morning everyone, Can someone reply who knows if Histogel causes any interference with IHC staining? Thank you in advance, Paula Bio-Path Med Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 10 May 2018 14:22:07 +0000 From: John Shelley To: dusko trajkovic , Paula Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] IHC and Histogel Message-ID: Content-Type: text/plain; charset="iso-8859-1" I agree with Dusko, I have used histogel the same way and have also used it for FISH and ISH without any issues. Kind Regards! ? John J Shelley Histology Core Manager Sanford Burnham Prebys Medical Discovery Institute at Lake Nona 6400 Sanger Road??????????????????????????????? Orlando, FL 32827??????????????????????????????????? Tel: (407) 745-2000 Ext.2517 Lab: (407) 745-2119 Fax: (407) 745-2001 ****email:? jshelley at SBPdiscovery.org ??****Please note email change -----Original Message----- From: dusko trajkovic via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, May 10, 2018 10:20 AM To: Paula; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC and Histogel We have used Histogel for years on cell pellets, and have not come across any IHC issues.Dusko On Thursday, May 10, 2018 7:13 AM, Paula via Histonet wrote: Hello good morning everyone, Can someone reply who knows if Histogel causes any interference with IHC staining? Thank you in advance, Paula Bio-Path Med Group Fountain Valley, CA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjshelley%40sbpdiscovery.org%7C217545f9b96c4e33e47a08d5b6812003%7C0b162723004547deb0699f1a7aa955a1%7C0%7C0%7C636615588083420177&sdata=LTCmir6KeMPW%2FGwPz5fdtjioshiu0OhqLYjhh6zJSPg%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjshelley%40sbpdiscovery.org%7C217545f9b96c4e33e47a08d5b6812003%7C0b162723004547deb0699f1a7aa955a1%7C0%7C0%7C636615588083420177&sdata=LTCmir6KeMPW%2FGwPz5fdtjioshiu0OhqLYjhh6zJSPg%3D&reserved=0 ------------------------------ Message: 6 Date: Thu, 10 May 2018 10:50:52 -0400 From: Charles Riley To: Histo List Subject: [Histonet] Shandon Veristain Gemini Autostainer Message-ID: Content-Type: text/plain; charset="UTF-8" Has anyone had any issues with this tissue stainer dropping or crushing racks? We use it to run our gyn and non gyn pap/thin prep slides. Recently it has been tipping over the slide racks in the middle of the procedure and breaking the slides -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Message: 7 Date: Thu, 10 May 2018 15:25:24 +0000 From: Histology To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] benchtop fume hoods Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD8206EB91B3 at vdc01.domain.local> Content-Type: text/plain; charset="us-ascii" Hi all, Can anyone share what type of benchtop fume hoods they are using in the lab? This would be for manual coverslipping and grossing. Thanks in advance! ------------------------------ Message: 8 Date: Thu, 10 May 2018 10:43:41 -0500 From: Mike Toole To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Shandon Veristain Gemini Autostainer Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB358CFE1F483 at mail> Content-Type: text/plain; charset="us-ascii" Charles, The issue is most likely the handle for the rack, The rack has a moveable handle. Make sure the handle is securely in the upright position. It has pins and holes that should line up and click into place. If it is even slightly at an angle or not securely snapped into an upright position it may bump into the side of the stain pots (and really cause a mess). Mike -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, May 10, 2018 9:51 AM To: Histo List Subject: [EXTERNAL] [Histonet] Shandon Veristain Gemini Autostainer Has anyone had any issues with this tissue stainer dropping or crushing racks? We use it to run our gyn and non gyn pap/thin prep slides. Recently it has been tipping over the slide racks in the middle of the procedure and breaking the slides -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 10 May 2018 12:38:28 -0400 From: Allan Wang Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] benchtop fume hoods Message-ID: Content-Type: text/plain; charset="UTF-8" I'd like to know as well. Thanks, Allan On Thu, May 10, 2018 at 11:25 AM, Histology via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all, > > Can anyone share what type of benchtop fume hoods they are using in the > lab? This would be for manual coverslipping and grossing. > > Thanks in advance! > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 174, Issue 7 **************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. From patpxs at gmail.com Thu May 10 21:52:31 2018 From: patpxs at gmail.com (P Sicurello) Date: Thu, 10 May 2018 19:52:31 -0700 Subject: [Histonet] benchtop fume hoods In-Reply-To: <09CFA3F99D5B2B42B88CDFB2FC4CFD8206EB91B3@vdc01.domain.local> References: <09CFA3F99D5B2B42B88CDFB2FC4CFD8206EB91B3@vdc01.domain.local> Message-ID: We are using a portable fume extractor from Sentry Air Systems for removing xylene fumes from our coverslipper. It's called the SS-200-WS, it has adjustable 'wings' and you can also buy one with shielding. We like it so much we bought another 5. The carbon filters make all the difference, our first filter lasted almost 1500 hours before needing replacement. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Thu, May 10, 2018 at 8:25 AM, Histology via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi all, > > Can anyone share what type of benchtop fume hoods they are using in the > lab? This would be for manual coverslipping and grossing. > > Thanks in advance! > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From redrose297 at gmail.com Fri May 11 07:45:16 2018 From: redrose297 at gmail.com (warda hassan) Date: Fri, 11 May 2018 16:45:16 +0400 Subject: [Histonet] Surepath V/s thin pre Message-ID: Hello to all Would like to know from experienced group who had worked on both systems surepath V/S thinprep which one is better and a comparison of the system on Turn Around Time, staffing & most important reporting. Many Thanks in advance Lujain From Neelam.Shinde at osumc.edu Fri May 11 07:56:11 2018 From: Neelam.Shinde at osumc.edu (Shinde, Neelam) Date: Fri, 11 May 2018 12:56:11 +0000 Subject: [Histonet] Picrosirius staining Message-ID: Hi, I have some collagen staining on mammry gland using picrosirius and I took images with a polarized scope. I want analyse the images and sure if image J is a good option. Can anyone suggest something better or how can I use image J to anlyse it. Thanks, Neelam. From jamie at watson-home.com Sat May 12 11:06:01 2018 From: jamie at watson-home.com (Jamie Watson) Date: Sat, 12 May 2018 16:06:01 +0000 Subject: [Histonet] Ethanol in tissue processing Message-ID: Hi All, Does anyone know what percentage of labs use ethanol vs. denatured alcohol or isopropyl alcohol on the tissue processor? Jamie From jmacdonald at mtsac.edu Sat May 12 14:48:40 2018 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Sat, 12 May 2018 19:48:40 +0000 Subject: [Histonet] Ethanol in tissue processing In-Reply-To: References: Message-ID: We use reagent alcohol on the processor and for staining. Get Outlook for iOS ________________________________ From: Jamie Watson via Histonet Sent: Saturday, May 12, 2018 9:06:01 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Ethanol in tissue processing Hi All, Does anyone know what percentage of labs use ethanol vs. denatured alcohol or isopropyl alcohol on the tissue processor? Jamie _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren at gmail.com Sat May 12 15:48:54 2018 From: jaylundgren at gmail.com (Jay Lundgren) Date: Sat, 12 May 2018 15:48:54 -0500 Subject: [Histonet] Ethanol in tissue processing In-Reply-To: References: Message-ID: Most places use "reagent alcohol" which, from most suppliers, is a mongrel blend of whatever is cheapest, and not even listed on the label. It's usually made up of a blend of ethanol, methanol, and isopropyl. Good luck finding what the percentages are. I've called one vendor before, and even they couldn't tell me. Virus-free. www.avg.com <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> On Sat, May 12, 2018 at 2:48 PM, Mac Donald, Jennifer via Histonet < histonet at lists.utsouthwestern.edu> wrote: > We use reagent alcohol on the processor and for staining. > > Get Outlook for iOS > ________________________________ > From: Jamie Watson via Histonet > Sent: Saturday, May 12, 2018 9:06:01 AM > To: Histonet at lists.utsouthwestern.edu > Subject: [Histonet] Ethanol in tissue processing > > Hi All, > > Does anyone know what percentage of labs use ethanol vs. denatured alcohol > or isopropyl alcohol on the tissue processor? > > Jamie > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From michael.gudo at morphisto.de Sat May 12 16:15:23 2018 From: michael.gudo at morphisto.de (Michael Gudo (Morphisto GmbH)) Date: Sat, 12 May 2018 23:15:23 +0200 Subject: [Histonet] Ethanol in tissue processing In-Reply-To: References: Message-ID: Well, in Germany, and as far as I know in Europe, most labs use ethanol at 96 and 99 percent as the last two steps in tissue processing. These ethanols are are denatured with MEK (= Butanon, Methylethylketon). Methanol or isopropyl is not allowed as denaturation agent in Europe, otherwise you have to pay the full alcohol tax. In our lab we we use 96 % ethanol and as last step and then, before going to xylene we use isopropanol which has a better dehydration effect than ethanol. 99 % ethanol takes water from the air and quite fast becomes a 96 or 95 % ethanol and you will have some water left in the specimens when you to to xylene. So our experience are that the best quality of dehydration can be reached with ethanol as high as possible and finally isopropanol before the xylene (2 steps). With best regards Michael > Am 12.05.2018 um 22:48 schrieb Jay Lundgren via Histonet : > > Most places use "reagent alcohol" which, from most suppliers, is a mongrel > blend of whatever is cheapest, and not even listed on the label. It's > usually made up of a blend of ethanol, methanol, and isopropyl. Good luck > finding what the percentages are. I've called one vendor before, and even > they couldn't tell me. > > > Virus-free. > www.avg.com > > <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> > > On Sat, May 12, 2018 at 2:48 PM, Mac Donald, Jennifer via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> We use reagent alcohol on the processor and for staining. >> >> Get Outlook for iOS >> ________________________________ >> From: Jamie Watson via Histonet >> Sent: Saturday, May 12, 2018 9:06:01 AM >> To: Histonet at lists.utsouthwestern.edu >> Subject: [Histonet] Ethanol in tissue processing >> >> Hi All, >> >> Does anyone know what percentage of labs use ethanol vs. denatured alcohol >> or isopropyl alcohol on the tissue processor? >> >> Jamie >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From histopathology_adolf at tut.by Sat May 12 16:56:50 2018 From: histopathology_adolf at tut.by (=?utf-8?B?0JjQstCw0L3QvtCyINCY0LLQsNC9?=) Date: Sun, 13 May 2018 00:56:50 +0300 Subject: [Histonet] (no subject) Message-ID: <7380481526162210@web60o.yandex.ru> -- ????????????????? ???? ??????????????????? ?????????????????? ???????????.?????? From rsrichmond at gmail.com Sat May 12 18:29:53 2018 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 12 May 2018 19:29:53 -0400 Subject: [Histonet] Histonet Digest, Vol 174, Issue 9 In-Reply-To: References: Message-ID: Jamie Watson, you need to clarify this question: >> Does anyone know what percentage of labs use ethanol vs. denatured alcohol or isopropyl alcohol on the tissue processor?<< 100% ethanol is perfectly OK, but it requires a lot of regulator compliance because you can drink it (though you shouldn't). The usual denatured alcohol for histology is customarily called "reagent alcohol". It contains 90% ethanol, 5% methanol, and 5% isopropanol. The BATF calls it "S3D modified". It's also possible to denature with methyl isobutyl ketone (MIBK) but it smells horrible. Alcohol denatured with acetone is available, but it'll extract your eosin - you need to know about it because an occasional manager will insist on it. All of these are equivalent from a practical viewpoint. Isopropyl alcohol (isopropanol) is of course quite different, and I have no idea how many labs use it. Bob Richmond Samurai Pathologist Maryville TN On Sat, May 12, 2018 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Ethanol in tissue processing (Jamie Watson) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 12 May 2018 16:06:01 +0000 > From: Jamie Watson > To: "Histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Ethanol in tissue processing > Message-ID: > namprd10.prod.outlook.com> > > Content-Type: text/plain; charset="us-ascii" > > Hi All, > > Does anyone know what percentage of labs use ethanol vs. denatured alcohol > or isopropyl alcohol on the tissue processor? > > Jamie > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 174, Issue 9 > **************************************** > From robert.jacox at thermofisher.com Sun May 13 09:29:47 2018 From: robert.jacox at thermofisher.com (Jacox, Robert A.) Date: Sun, 13 May 2018 14:29:47 +0000 Subject: [Histonet] Ethanol in tissue processing In-Reply-To: References: , Message-ID: Jay, Michael's post is correct and very well stated. In the US we estimate between 80 -85% of labs now use and RGA. The most common blend in the U.S is 95-5 which is 95% ethanol denatured with 5% methanol. Any company you purchase from should be happy to provide a C of A (certificate of analysis) to verify how they denature. Hope this helps. Robert Jacox Product Manager Thermo Fisher Scientific Sent from my iPad > On May 12, 2018, at 5:21 PM, Michael Gudo (Morphisto GmbH) via Histonet wrote: > > CAUTION: This email originated from outside of the organization. Do not click links or open attachments unless you recognize the sender and know the content is safe. > > > Well, in Germany, and as far as I know in Europe, most labs use ethanol at 96 and 99 percent as the last two steps in tissue processing. These ethanols are are denatured with MEK (= Butanon, Methylethylketon). Methanol or isopropyl is not allowed as denaturation agent in Europe, otherwise you have to pay the full alcohol tax. > > In our lab we we use 96 % ethanol and as last step and then, before going to xylene we use isopropanol which has a better dehydration effect than ethanol. 99 % ethanol takes water from the air and quite fast becomes a 96 or 95 % ethanol and you will have some water left in the specimens when you to to xylene. > So our experience are that the best quality of dehydration can be reached with ethanol as high as possible and finally isopropanol before the xylene (2 steps). > > With best regards > Michael > > >> Am 12.05.2018 um 22:48 schrieb Jay Lundgren via Histonet : >> >> Most places use "reagent alcohol" which, from most suppliers, is a mongrel >> blend of whatever is cheapest, and not even listed on the label. It's >> usually made up of a blend of ethanol, methanol, and isopropyl. Good luck >> finding what the percentages are. I've called one vendor before, and even >> they couldn't tell me. >> >> >> Virus-free. >> www.avg.com >> >> <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> >> >> On Sat, May 12, 2018 at 2:48 PM, Mac Donald, Jennifer via Histonet < >> histonet at lists.utsouthwestern.edu> wrote: >> >>> We use reagent alcohol on the processor and for staining. >>> >>> Get Outlook for iOS >>> ________________________________ >>> From: Jamie Watson via Histonet >>> Sent: Saturday, May 12, 2018 9:06:01 AM >>> To: Histonet at lists.utsouthwestern.edu >>> Subject: [Histonet] Ethanol in tissue processing >>> >>> Hi All, >>> >>> Does anyone know what percentage of labs use ethanol vs. denatured alcohol >>> or isopropyl alcohol on the tissue processor? >>> >>> Jamie >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=gOuPKZIWz4dF3ZFLLr9VlODKDl2IATmOotwBCEAUpbM&e= >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=gOuPKZIWz4dF3ZFLLr9VlODKDl2IATmOotwBCEAUpbM&e= >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=gOuPKZIWz4dF3ZFLLr9VlODKDl2IATmOotwBCEAUpbM&e= > > ************************************************************************************************ > MORPHISTO GmbH > PD Dr. phil. nat. Michael Gudo > Weism?llerstr. 45 > 60314 Frankfurt am Main > Telefon: 069 / 400 3019 - 62 > Telefax: 069 / 400 3019 - 64 > > E-Mail: michael.gudo at morphisto.de > Internet: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.morphisto.de_&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=W1YneELMXXshKSQu4jJSvRn53FG5LC6-E-xdlQ4K4pQ&e= > > Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo > > Registergericht: Amtsgericht Frankfurt > Registernummer: HRB 74954 > Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 > ************************************************************************************************ > Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. > ************************************************************************************************ > This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=gOuPKZIWz4dF3ZFLLr9VlODKDl2IATmOotwBCEAUpbM&e= From jamie at watson-home.com Sun May 13 12:04:51 2018 From: jamie at watson-home.com (Jamie Watson) Date: Sun, 13 May 2018 17:04:51 +0000 Subject: [Histonet] Ethanol in tissue processing In-Reply-To: References: , Message-ID: Thank you all for your answers, our RG alcohol is 3% methanol. You are confirming what I thought, we are using the same type of alcohol as most histology labs. Jamie On May 13, 2018 7:37:52 AM "Jacox, Robert A. via Histonet" wrote: > Jay, > > Michael's post is correct and very well stated. In the US we estimate > between 80 -85% of labs now use and RGA. The most common blend in the U.S > is 95-5 which is 95% ethanol denatured with 5% methanol. Any company you > purchase from should be happy to provide a C of A (certificate of analysis) > to verify how they denature. > > Hope this helps. > > Robert Jacox > Product Manager > Thermo Fisher Scientific > > Sent from my iPad > >> On May 12, 2018, at 5:21 PM, Michael Gudo (Morphisto GmbH) via Histonet >> wrote: >> >> CAUTION: This email originated from outside of the organization. Do not >> click links or open attachments unless you recognize the sender and know >> the content is safe. >> >> >> Well, in Germany, and as far as I know in Europe, most labs use ethanol at >> 96 and 99 percent as the last two steps in tissue processing. These >> ethanols are are denatured with MEK (= Butanon, Methylethylketon). Methanol >> or isopropyl is not allowed as denaturation agent in Europe, otherwise you >> have to pay the full alcohol tax. >> >> In our lab we we use 96 % ethanol and as last step and then, before going >> to xylene we use isopropanol which has a better dehydration effect than >> ethanol. 99 % ethanol takes water from the air and quite fast becomes a 96 >> or 95 % ethanol and you will have some water left in the specimens when you >> to to xylene. >> So our experience are that the best quality of dehydration can be reached >> with ethanol as high as possible and finally isopropanol before the xylene >> (2 steps). >> >> With best regards >> Michael >> >> >>> Am 12.05.2018 um 22:48 schrieb Jay Lundgren via Histonet >>> : >>> >>> Most places use "reagent alcohol" which, from most suppliers, is a mongrel >>> blend of whatever is cheapest, and not even listed on the label. It's >>> usually made up of a blend of ethanol, methanol, and isopropyl. Good luck >>> finding what the percentages are. I've called one vendor before, and even >>> they couldn't tell me. >>> >>> >>> Virus-free. >>> www.avg.com >>> >>> <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> >>> >>> On Sat, May 12, 2018 at 2:48 PM, Mac Donald, Jennifer via Histonet < >>> histonet at lists.utsouthwestern.edu> wrote: >>> >>>> We use reagent alcohol on the processor and for staining. >>>> >>>> Get Outlook for >>>> iOS >>>> ________________________________ >>>> From: Jamie Watson via Histonet >>>> Sent: Saturday, May 12, 2018 9:06:01 AM >>>> To: Histonet at lists.utsouthwestern.edu >>>> Subject: [Histonet] Ethanol in tissue processing >>>> >>>> Hi All, >>>> >>>> Does anyone know what percentage of labs use ethanol vs. denatured alcohol >>>> or isopropyl alcohol on the tissue processor? >>>> >>>> Jamie >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet at lists.utsouthwestern.edu >>>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=gOuPKZIWz4dF3ZFLLr9VlODKDl2IATmOotwBCEAUpbM&e= >>>> _______________________________________________ >>>> Histonet mailing list >>>> Histonet at lists.utsouthwestern.edu >>>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=gOuPKZIWz4dF3ZFLLr9VlODKDl2IATmOotwBCEAUpbM&e= >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=gOuPKZIWz4dF3ZFLLr9VlODKDl2IATmOotwBCEAUpbM&e= >> >> ************************************************************************************************ >> MORPHISTO GmbH >> PD Dr. phil. nat. Michael Gudo >> Weism?llerstr. 45 >> 60314 Frankfurt am Main >> Telefon: 069 / 400 3019 - 62 >> Telefax: 069 / 400 3019 - 64 >> >> E-Mail: michael.gudo at morphisto.de >> Internet: >> https://urldefense.proofpoint.com/v2/url?u=http-3A__www.morphisto.de_&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=W1YneELMXXshKSQu4jJSvRn53FG5LC6-E-xdlQ4K4pQ&e= >> >> >> Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo >> >> Registergericht: Amtsgericht Frankfurt >> Registernummer: HRB 74954 >> Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 >> ************************************************************************************************ >> Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder >> dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der >> unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder >> Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht >> der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten >> wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und >> anschliessend diese Email und saemtliche Anhaenge zu loeschen. >> ************************************************************************************************ >> This message is exclusively for the person addressed or their >> representative. Any form of the unauthorized use, publication, >> reproduction, copying or disclosure of the content of this e-mail is not >> permitted. If you are not the intended recipient of this message and its >> contents, please notify this sender immediately and delete this message and >> all its attachments subsequently. >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=Ok6Bw_gcp2zI5CS04j2rJz6wZnfzU8hCIsEVcthsaRI&m=mAT0wnfpNJE6HRWnB402WwQGF4ORbjXZON61CCMao9s&s=gOuPKZIWz4dF3ZFLLr9VlODKDl2IATmOotwBCEAUpbM&e= > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Sent with AquaMail for Android https://www.mobisystems.com/aqua-mail From Kim.Kolman at va.gov Mon May 14 11:59:46 2018 From: Kim.Kolman at va.gov (Kolman, Kimberly D.) Date: Mon, 14 May 2018 16:59:46 +0000 Subject: [Histonet] cervical biopsy method Message-ID: Looking for any folks out there who have experience with cervical biopsies obtained using the "SpiraBrush" technique. It is from the Histologics Company and employs a fabric 'brush' to obtain the sample instead of the standard punch instrument. One of our gynecologists would like to begin using it here to enhance samples; I am unfamiliar with it; my pathologist as well. We wonder how labor-intensive it is to retrieve the fragments and how they well process. My understanding is that the fragments would be very tiny; it sounds very unlikely to be able to orient them during embedding..... Any input appreciated! Thanks, Kim Kimberly D. Kolman, HT (ASCP) VA Eastern Kansas Health Care System Dwight D. Eisenhower VA Medical Center 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 913-682-2000 x 52537 Fax: 913-758-4193 Kim.kolman at va.gov From jwwalker at rrmc.org Mon May 14 12:24:24 2018 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Mon, 14 May 2018 17:24:24 +0000 Subject: [Histonet] Surepath V/s thin pre In-Reply-To: References: Message-ID: Lujain, You'd have to define what "better" mean to you and your laboratory. I've used both systems in my career. Each have their pros and cons. You would also need to define the specimen types you intend to process, i.e. Pap tests only, Pap tests and non-gyn. Each company now offers a variety of options to help with pre-processing and processing steps. Other variables to think about is if you are using the system for Pap tests, are you planning ancillary testing from the vial, do providers in your area have a preference, i.e ThinPrep is FDA approved as significantly better than conventional Pap tests, SurePath has same screening results as conventional but with fewer unsatisfactory Pap results. Depending on your volume, maybe you want to offer both so that your providers have a choice of Pap testing options. Lastly, are any of your providers looking for HPV primary screening as your choices are limited for this testing offer. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790? F 802.747.6525 joewalker at rrmc.org, www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Our Commitment to our Community: We Listen, We Respect, We Care . . . Always. Joint Commission Accredited | Best Regional Hospital: U.S. News & World Report | Leapfrog Hospital Safety A Rating ANCC Magnet Hospital Designation? | Healthgrades: Excellence Award for Patient Safety | Healthgrades: Outstanding Patient Experience Award -----Original Message----- From: warda hassan via Histonet Sent: Friday, May 11, 2018 8:45 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Surepath V/s thin pre Hello to all Would like to know from experienced group who had worked on both systems surepath V/S thinprep which one is better and a comparison of the system on Turn Around Time, staffing & most important reporting. Many Thanks in advance Lujain _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7Cbb383c8f15f3420c758008d5b73d3b25%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C636616395978299757&sdata=VMvIGE9kKtYMevoXtxiKuzPhVAVzMK2dFpITWBtI4FA%3D&reserved=0 From tbraud at holyredeemer.com Mon May 14 13:48:42 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 14 May 2018 18:48:42 +0000 Subject: [Histonet] Ventana dispenser issues - AGAIN Message-ID: <48E053DDF6CE074DB6A7414BA05403F8C1B9455F@HRHEX02-HOS.holyredeemer.local> When will this company ever learn? I'm at my wit's end with failed dispensers from Roche Ventana. The volume of time I've wasted on this issue with this company is monumental. They refuse to give true tracking information on the dispensers. We have a relatively new Ultra. The instrument is lovely, but the stains completely unreliable because of the dispenser failure. Worse yet, one can never tell when the dispenser will fail. It might fail right out of the box, or work fine for 30 tests and fail on 31. This problem has been going on since the end of 2017 and still is continuing. I know I'm not alone because of the recalls and most recently, the Medical Device Ongoing Action published 5/11/2018. Reaching out to the company is like trying to talk to a politician who talks in circles. I'm truly at a loss and would welcome any suggestions from non-Roche employees. Frustrated, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor From jwwalker at rrmc.org Mon May 14 15:24:02 2018 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Mon, 14 May 2018 20:24:02 +0000 Subject: [Histonet] Surepath V/s thin pre In-Reply-To: References: Message-ID: What is you unsat rate with the ThinPrep? Published rates are as follows. Are lab runs around 1.5% for ThinPrep. CATEGORY 5th 10th 25th 50th 75th 90th 95th UNSATISFACTORY (%) 0.3 0.4 0.7 1.8 2.5 3.2 3.8 It is also important to recognize that unsatisfactory Pap test results carry an increased risk of underlying disease. One large study found that up to 26% of unsatisfactory specimens were from patients who had atypical squamous cells (ASC) or a squamous intraepithelial lesion (SIL)1. This translates into your patient having a 4-time greater risk of cervical intraepithelial neoplasia (CIN) 2/3 than patients with a normal Pap test result2. Clinical correlation and patient examination should be the first steps in delineating the cause of the unsatisfactory diagnosis1. The American Society for Colposcopy and Cervical Pathology (ASCCP) recommends repeating the Pap test on all patients, with the exception of a patient with known HPV positive status. In these cases, colposcopy could be considered as an alternative to repeat Pap testing. The suggested time interval for repeat Pap testing is 2 to 4 months. We tell our providers that they can help decrease the unsatisfactory rate by following these guidelines: Avoid scheduling or obtaining a Pap test while your patient is menstruating. While the test can be collected, it is best to avoid it during this time. During the 2 days prior to the Pap Test, please refrain from: Intercourse Douching Birth control gels, foam or creams Vaginal medications or creams unless being utilize to help obtain the Pap test Use of tampons Use of personal lubricants If a lubricant is utilized for the collection of the Pap test, please make sure that ?carbomer? or ?Carbopol? are not in the ingredient list. Safe lubricants include: KY Jelly? Surgilube? Astroglide? Crystelle? References: 1. Ransdell, et. al., Clinicopathologic Correlation of the Unsatisfactory Papanicolaou Smear, Cancer Cytopathology, 1997; 81(3)139-143 2. Nyg?rd et al. CIN 2/3 and cervical cancer in an organized screening programme after an unsatisfactory or a normal Pap smear: a seven-year prospective study of the Norwegian population-based screening programme, J. Med Screen,11 (2): 70. (2004) The biggest con to Surepath is that there are a lot of preprocessing steps involved that include centrifugation, resuspension, etc for the cell enrichment to occur. They do now make a processor that reduces the amount of hands on time with the specimen but this comes at a cost. The system also takes up a lot of space compared to a couple of T2000 processors if that is what you have in your lab. As mentioned, if you are moving to Surepath, make sure that you will still be able to offer the same out of the vial testing that you are currently offering on ThinPreps. Ancillary testing for HPV, chlamydia, gonorrhea and trichomonas are all available from the ThinPrep vial and not necessarily from SurePath. This may mean your clinicians may have to co collect two or three specimens which can lead to patient dissatisfaction. From a cytotech?s viewpoint, the SP and TP are both similar to review. The presentation of the cells are a little different and may require a slight adjustment period for your techs. The biggest complaint I have heard from a cytotech and pathologist view point is that cells appear in multiple focal plans that can sometimes make the cells in question a little bit of a challenge to interpret. Making a large switch like this is a big challenge but possible. By best advice would be to closely develop a list of pros and cons that you have with your current system and compare what the new system will either fix for you or will create more issues. Neither system is ?perfect? and you have to consider your space, workflows, billing and reimbursement within your payor mix, and patient, clinician, pathologist satisfaction. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewalker at rrmc.org, www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Our Commitment to our Community: We Listen, We Respect, We Care . . . Always. Joint Commission Accredited | Best Regional Hospital: U.S. News & World Report | Leapfrog Hospital Safety A Rating ANCC Magnet Hospital Designation? | Healthgrades: Excellence Award for Patient Safety | Healthgrades: Outstanding Patient Experience Award From: warda hassan Sent: Monday, May 14, 2018 1:52 PM To: Joe W. Walker, Jr. Subject: Re: [Histonet] Surepath V/s thin pre Dear Joe W. Walker, Thank you for your feedback on and to pinpoint on areas of interest which requires to be thought on. We are considering moving totally toward Surepath due to high number of unsatisfactory per year and that is basically from our clinical side NOT due to system. So if you can share with me your experience on Surepath how it has been and what are pros and cons i would be great full. Wishing you a nice day Lujain On Mon, May 14, 2018 at 9:24 PM Joe W. Walker, Jr. > wrote: Lujain, You'd have to define what "better" mean to you and your laboratory. I've used both systems in my career. Each have their pros and cons. You would also need to define the specimen types you intend to process, i.e. Pap tests only, Pap tests and non-gyn. Each company now offers a variety of options to help with pre-processing and processing steps. Other variables to think about is if you are using the system for Pap tests, are you planning ancillary testing from the vial, do providers in your area have a preference, i.e ThinPrep is FDA approved as significantly better than conventional Pap tests, SurePath has same screening results as conventional but with fewer unsatisfactory Pap results. Depending on your volume, maybe you want to offer both so that your providers have a choice of Pap testing options. Lastly, are any of your providers looking for HPV primary screening as your choices are limited for this testing offer. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewalker at rrmc.org, www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Our Commitment to our Community: We Listen, We Respect, We Care . . . Always. Joint Commission Accredited | Best Regional Hospital: U.S. News & World Report | Leapfrog Hospital Safety A Rating ANCC Magnet Hospital Designation? | Healthgrades: Excellence Award for Patient Safety | Healthgrades: Outstanding Patient Experience Award -----Original Message----- From: warda hassan via Histonet > Sent: Friday, May 11, 2018 8:45 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Surepath V/s thin pre Hello to all Would like to know from experienced group who had worked on both systems surepath V/S thinprep which one is better and a comparison of the system on Turn Around Time, staffing & most important reporting. Many Thanks in advance Lujain _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7Cbb383c8f15f3420c758008d5b73d3b25%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C636616395978299757&sdata=VMvIGE9kKtYMevoXtxiKuzPhVAVzMK2dFpITWBtI4FA%3D&reserved=0 From algranth at email.arizona.edu Tue May 15 11:16:32 2018 From: algranth at email.arizona.edu (Grantham, Andrea L - (algranth)) Date: Tue, 15 May 2018 16:16:32 +0000 Subject: [Histonet] Embedding molds Message-ID: I am doing some part time work in a lab and there is an embedding center but the larger metal molds and some of the small square ones have gone missing. Does anybody have any of these that they aren?t using and would you be willing to part with them? Andi Grantham University of Arizona From twebster at CRH.org Tue May 15 12:18:59 2018 From: twebster at CRH.org (Webster, Thomas S.) Date: Tue, 15 May 2018 17:18:59 +0000 Subject: [Histonet] Surepath V/s thin pre Message-ID: <7207186ED68FB542803CAF1CE6E82FF897918A56@exmb2.crh.org> Thinprep has a higher unsatisfactory rate for Pap tests. The filters get clogged up and cells end up stuck in the vial. Someone in the lab will need to dedicate time to reprocessing unsatisfactory Thinprep specimens if you choose them. If Paps are affected then it isn't a stretch to assume Non-gyns aren't as cellular as they could be sometimes due to material clogging the filter up. We have the old T2000 processor for Thinprep which is very time consuming. Our volume has dropped so much that there is no chance of getting one of their newer processors. HPV primary screening was one advantage Thinprep had, but now BD (Surepath) has an FDA approved HPV test for primary screening so that advantage is gone. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From Neelam.Shinde at osumc.edu Tue May 15 14:06:06 2018 From: Neelam.Shinde at osumc.edu (Shinde, Neelam) Date: Tue, 15 May 2018 19:06:06 +0000 Subject: [Histonet] confirm 49d28cab5d778309f7141ce2de0e5e20223b7736 Message-ID: From relia1 at earthlink.net Wed May 16 11:59:26 2018 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 16 May 2018 12:59:26 -0400 Subject: [Histonet] Summer is Almost Here!! Message-ID: <000001d3ed37$3f60f7a0$be22e6e0$@earthlink.net> Hi Histonetters, Summer is Almost HERE! I can tell the humidity and the afternoon showers have arrived!! #summerinflorida I am looking forward to beating the heat with some amazing beach days!!! So what are you doing for your Summer Vacation? . Going somewhere exciting? . A Staycation? . Visiting Family or Friends? . Attending a Graduation, A Wedding or Family Reunion? Visiting anyplace you might consider relocation to? If so drop me a line and let me know so I can make a note to keep you posted on positions in that area. Even if we have talked recently drop me a line and remind me!! I do have some exciting opportunities that might coincide with your plans and if not I do have new opportunities coming in DAILY!!! I have opportunities in Texas, California, Ohio, Alabama and Tampa, Florida All of my clients offer excellent compensation, benefits and some offer relocation assistance and or sign on bonuses. All of these jobs are full time & permanent NEW POSITIONS ARE COMING IN DAILY!!! So Drop me a line and let me know where you want to go!! Histonetters, if you or anyone you know is interested in hearing more about any of these opportunities please contact me. Remember if I place someone you refer to me you will earn a referral bonus!!! Is your lab shorthanded? Did you know that if I get the job opening with your help and place someone in the position you will also earn a referral fee? Ask me how!!!! I can be reached anytime on my cell/text at 407-353-5070 or toll free at 866-607-3542 or e-mail me at relia1 at earthlink.net Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Terry.Dyer at tmmc.com Wed May 16 12:38:04 2018 From: Terry.Dyer at tmmc.com (Dyer, Terry) Date: Wed, 16 May 2018 17:38:04 +0000 Subject: [Histonet] Ventana dispenser issues - AGAIN In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8C1B9455F@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8C1B9455F@HRHEX02-HOS.holyredeemer.local> Message-ID: <52B7E4673B4A6045988BC7065CA3B848834C0538@EXCH-2.tmmc.com> Hello, Once again we are having issues also. I called Roche to ask if there are issues with dispensers again and they said there are not. We had intermittent staining on a Ultra. We checked all of our dispensers and discovered that the OV Link dispenser was difficult to plunge so I removed it and changed out the solution to another light sensitive dispenser just to see if that was the issue and it was. The remainder of the kit worked without any trouble. I know this is not the appropriate thing to do but I wanted to know if it was the ULTRA or the dispenser. I relayed this to Roche and they said that I void the replacement because I changed out the dispenser, but at least it is documented. If we are having any problems we call Roche and document everything on their end and ours. These are great instruments but we agree the staining inconsistency is an issue. Pathologists get frustrated plus the extra repeat staining and turnaround time adds up. Hopefully everyone calls and documents. If they do not hear from everyone they won't feel that it is an issue. Just my thoughts. Terry Dyer, HT(ASCP) IHC Specialist -----Original Message----- From: Terri Braud [mailto:tbraud at holyredeemer.com] Sent: Monday, May 14, 2018 11:49 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Ventana dispenser issues - AGAIN When will this company ever learn? I'm at my wit's end with failed dispensers from Roche Ventana. The volume of time I've wasted on this issue with this company is monumental. They refuse to give true tracking information on the dispensers. We have a relatively new Ultra. The instrument is lovely, but the stains completely unreliable because of the dispenser failure. Worse yet, one can never tell when the dispenser will fail. It might fail right out of the box, or work fine for 30 tests and fail on 31. This problem has been going on since the end of 2017 and still is continuing. I know I'm not alone because of the recalls and most recently, the Medical Device Ongoing Action published 5/11/2018. Reaching out to the company is like trying to talk to a politician who talks in circles. I'm truly at a loss and would welcome any suggestions from non-Roche employees. Frustrated, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor From criley at dpspa.com Thu May 17 12:34:17 2018 From: criley at dpspa.com (Charles Riley) Date: Thu, 17 May 2018 13:34:17 -0400 Subject: [Histonet] Leica open containers Message-ID: How long does everyone leave an antibody in an open container once its been concentrated? Trying to determine why my IHC' become weak randomly. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From oneilb at wvumedicine.org Thu May 17 13:27:34 2018 From: oneilb at wvumedicine.org (Oneil, Beth) Date: Thu, 17 May 2018 18:27:34 +0000 Subject: [Histonet] Ventana dispenser issues - boogers Message-ID: <3CEB8EBCF9C7A648B9694B5696462A718AA9B529@NT-EX1.wvuhs.com> In replying to the comments about the Ventana dispenser issues, we have to routinely check the dispensers for boogers. Especially the Optiview multimers & amplifiers, and the Ultraview amplifiers. We pick them out with toothpicks daily because they will have a gelatinous plug or little crusty which inhibits proper dispensing. Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC Histology Supervisor, Technical Specialist WVU Hospital Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From boznpl at aol.com Thu May 17 13:59:16 2018 From: boznpl at aol.com (Laurie Colbert) Date: Thu, 17 May 2018 14:59:16 -0400 Subject: [Histonet] p16 Antibody Message-ID: <1636f78784f-c8a-34e@webjasstg-vab16.srv.aolmail.net> We have been using Ventana's p16 antibody and detection kit on an IHC stainer that is not Ventana's stainer.? We have been told that these reagents are now obsolete, but that they can be purchased in dispensers which are customized for Ventana's own stainer.? Can I simply just dispense their reagents into my own vials for my stainer?? I'm wondering if anyone else is dealing with this same issue. Are there other companies that sell the p16 antibody?? The last time I checked (which was years ago), Ventana was the only one selling this. Thanks, Laurie Redmond Path MD Los Angeles, CA From Audrey.Pagan at nyumc.org Thu May 17 15:06:17 2018 From: Audrey.Pagan at nyumc.org (Pagan, Audrey) Date: Thu, 17 May 2018 20:06:17 +0000 Subject: [Histonet] Opening for Pathology Case Coordinator at NYULangone Health Message-ID: Pathology Case Coordinator Position Number 10047343 We have an exciting opportunity to join the NYU Langone Medical Center team. To qualify you must have a Bachelor's Degree with two years of experience as a laboratory technician or a technologist. Candidates must be able to effectively communicate with all levels of the organization. Primary responsibilities; provide timely resolution for reported non-conformances, work on an inter-laboratory, inter-personal, and inter-departmental level to resolve these issues as applicable; collect error tracking and QC data from the entire department; track and record errors, create event reports, perform follow up audits to assess compliance with corrective action and assist with additional special projects as requested. New York State technical license is not required. If you are interested please contact: Dana Warfield Assistant Director, Quality Systems Department of Pathology dana.warfield at nyumc.org 212.263.5471 Tisch Hospital TH412 NYU Langone Medical Center 560 First Avenue NY, NY 10016 ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= From greg.dobbin at gmail.com Fri May 18 12:25:21 2018 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Fri, 18 May 2018 14:25:21 -0300 Subject: [Histonet] p16 Antibody Message-ID: Hi Laurie, We are purchasing the antibody only from Roche/Ventana and optimizing it for use with our detection system (Bond Refine Detection kit) and the Bond-III stainer. Ask your Ventana person about buying the Ab only. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From greg.dobbin at gmail.com Fri May 18 12:35:04 2018 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Fri, 18 May 2018 14:35:04 -0300 Subject: [Histonet] Leica open containers Message-ID: Hi Charles, If you are using the Leica Diluting Buffer or another commercially prepared diluting buffer the antibody should remain stable for a very long time. I can't say how long, I suspect there would be numerous variables to be considered but generally...I would expect very little loss of intensity over two years even. For me, the antibody expires long before the any deterioration of signal is noticed. In your case, if you are using a commercially prepared diluent, then start looking at extended times left out of the fridge, a malfunctioning fridge, contamination, etc. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From dgoodwin at princetonhcs.org Sat May 19 09:47:04 2018 From: dgoodwin at princetonhcs.org (goodwin, diana) Date: Sat, 19 May 2018 14:47:04 +0000 Subject: [Histonet] p16 Antibody In-Reply-To: <1636f78784f-c8a-34e@webjasstg-vab16.srv.aolmail.net> References: <1636f78784f-c8a-34e@webjasstg-vab16.srv.aolmail.net> Message-ID: <056adf61eb574e34b59abef8c194b853@EX-MB-02.PHCS.ORG> Hi, Laurie. We use the Leica Bond and dispense it into an open vial for use on that instrument. I suggest you contact the vendor for the IHC stainer you are using. They should be able to tell you how to optimize the new Ventana Cintec P16 for use on your existing system. Good Luck! Diana Goodwin Anatomic Pathology Supervisor Penn Medicine Princeton Medical Center Ph: 609.853.6808 Fax: 609.853.6841 -----Original Message----- From: Laurie Colbert [mailto:boznpl at aol.com] Sent: Thursday, May 17, 2018 2:59 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] p16 Antibody We have been using Ventana's p16 antibody and detection kit on an IHC stainer that is not Ventana's stainer.? We have been told that these reagents are now obsolete, but that they can be purchased in dispensers which are customized for Ventana's own stainer.? Can I simply just dispense their reagents into my own vials for my stainer?? I'm wondering if anyone else is dealing with this same issue. Are there other companies that sell the p16 antibody?? The last time I checked (which was years ago), Ventana was the only one selling this. Thanks, Laurie Redmond Path MD Los Angeles, CA This e-mail transmission and any documents attached hereto contain information from Princeton HealthCare System which is confidential and/or legally privileged. This information is intended only for the use of the individual or entity to which it is addressed, and only for the purpose for which this transmission was made. Any other use, disclosure, copying, distribution, re-transmission, or the taking of any action in reliance upon the contents of this information is strictly prohibited. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, re-transmission or the taking of any action in reliance upon the contents of this information is strictly prohibited, and that this e-mail transmission and accompanying documents should be returned to Princeton HealthCare System immediately. If you have received this e-mail transmission in error, please notify the sender immediately and destroy all copies of the information contained in this transmission. The above signature block is intended for identification purposes only and does not constitute affirmation that a binding contract has been created via this e-mail communication unless expressly so stated within the body of this e-mail transmission. From c.tague at Pathologyarts.com Sat May 19 12:59:03 2018 From: c.tague at Pathologyarts.com (Curt) Date: Sat, 19 May 2018 17:59:03 +0000 Subject: [Histonet] tissue blocks available for banking Message-ID: <9C8F910F72893643B3C3793C3D67132B68BDA9BA@PATHOLOGYSERVER.pathologyarts.local> If anyone is looking for tissue for a bank, I have a boxes and boxes of derm specimens and GI specimens. I know they're probably not what everyone needs but if someone out there does need them, you can have them. figured I'd ask before I disposed of them... at the landfill next to the city water well... LOL, just kidding, I know someone out there will not think that's funny, it's just a joke. Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. From koellingr at comcast.net Sat May 19 14:06:38 2018 From: koellingr at comcast.net (RAY KOELLING) Date: Sat, 19 May 2018 12:06:38 -0700 (PDT) Subject: [Histonet] histo-off topic Message-ID: <578292945.1410691.1526756798387@connect.xfinity.com> Hello all, returned late last night from Pittsburg and the Intel-International Science and Engineering Fair, May 13-18. The largest most prestigious pre-college STEM fair in the world. Took four high school students from the Spokane region. One of our other "Team Washington" students from near Seattle won the second place prize for the entire 1,400 project fair from 81 countries and regions. A $50,000 scholarship with a chemical engineering project. While there, I viewed or walked by every project. Saw 50-60 with histology images/histology projects. IF, IHC, H&E, Safrinin O, FISH, PAS, decals, frozen, TMA's; anything and everything we all know. On osteoarthritis, breast cancer, kidney disease, diabetic pancreas, neuro, lymphoma's and more. If you are able to help what could be a potential world class student in the field of histology/pathology/medical and clinical research, hope you will. Sorry I was never able to get to the local histology school while my students were being judged in Convention Center. Couldn't seem to ever contact them. Ray Koelling Eastern Washington Regional Science and Engineering Fair, Fair Director Washington State Science and Engineering Fair, Board Member lecture faculty, University of Washington School of Medicine, Spokane-WWAMI site From gordon at 10db.co.uk Sat May 19 13:49:45 2018 From: gordon at 10db.co.uk (Gordon Brown) Date: Sat, 19 May 2018 20:49:45 +0200 Subject: [Histonet] Histopathology text recommendations Message-ID: <4cf2q0p06jig4l65brthlbss.1526755785375@email.android.com> Can anyone give me recommendations for a good histopathology text and atlas? I'm not a professional or even a formal student, I'm an amateur microscopist with a keen interest in histology, although I did attend a UK medical school many years ago only to decide that medicine was not for me. I'm now retired and expanding my interest in microscopy, which has fascinated me since the age of 8, I now have a collection of decent microscopes and microtomes and I'm setting up my study as a home microscopy and slide making facility. I have a growing collection of old histology slides including a number of pathology examples and I'm keen to be able to identify the histological changes that take place due to disease. In particular I am looking to be able to identify and photograph carcinomas as I recently acquired a photomicrography bench used by the Imperial Cancer Research Campaign in London during the 1950s and 1960s, and it would be appropriate to use it for the purpose it was designed for. Hope someone can help, I've subscribed to this list for many years but only rarely posted anything, the last time was when I enquired about replaceable blade holders for the original Cambridge rocking microtome and a very kind histologist in the USA sent me a slightly damaged holder and some blades. Fortunately I never managed to adapt it for the Cambridge as it is a perfect fit for the Reichert and Leitz rotary microtomes I now own! From saundra.ellis at floridawomancare.com Sat May 19 16:17:25 2018 From: saundra.ellis at floridawomancare.com (Saundra Ellis) Date: Sat, 19 May 2018 21:17:25 +0000 Subject: [Histonet] Histopathology text recommendations In-Reply-To: <4cf2q0p06jig4l65brthlbss.1526755785375@email.android.com> References: <4cf2q0p06jig4l65brthlbss.1526755785375@email.android.com> Message-ID: Anything written by Freida Carson. Saundra Ellis Histology Supervisor Florida Woman Care Laboratory Cell (360) 513-9665 (best contact) FAX (813) 433-5542 saundra.ellis at floridawomancare.com ________________________________ From: Gordon Brown via Histonet Sent: Saturday, May 19, 2018 2:49:45 PM To: histonet at lists.utsouthwestern.edu Cc: Gordon Brown Subject: [Histonet] Histopathology text recommendations Can anyone give me recommendations for a good histopathology text and atlas? I'm not a professional or even a formal student, I'm an amateur microscopist with a keen interest in histology, although I did attend a UK medical school many years ago only to decide that medicine was not for me. I'm now retired and expanding my interest in microscopy, which has fascinated me since the age of 8, I now have a collection of decent microscopes and microtomes and I'm setting up my study as a home microscopy and slide making facility. I have a growing collection of old histology slides including a number of pathology examples and I'm keen to be able to identify the histological changes that take place due to disease. In particular I am looking to be able to identify and photograph carcinomas as I recently acquired a photomicrography bench used by the Imperial Cancer Research Campaign in London during the 1950s and 1960s, and it would be appropriate to use it for the purpose it was designed for. Hope someone can help, I've subscribed to this list for many years but only rarely posted anything, the last time was when I enquired about replaceable blade holders for the original Cambridge rocking microtome and a very kind histologist in the USA sent me a slightly damaged holder and some blades. Fortunately I never managed to adapt it for the Cambridge as it is a perfect fit for the Reichert and Leitz rotary microtomes I now own! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this transmission may be privileged and confidential, as it is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this transmission in error, please immediately reply to the sender that you have received this communication in error and then delete it. Thank you. CONFIDENTIALITY NOTICE: The information contained in this transmission may be privileged and confidential, as it is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this transmission in error, please immediately reply to the sender that you have received this communication in error and then delete it. Thank you. From cls71877 at gmail.com Sat May 19 18:53:12 2018 From: cls71877 at gmail.com (Cristi Rigazio) Date: Sat, 19 May 2018 19:53:12 -0400 Subject: [Histonet] Histopathology text recommendations In-Reply-To: References: <4cf2q0p06jig4l65brthlbss.1526755785375@email.android.com> Message-ID: <37037DD4-E107-4CA7-A4DE-6C4D013BCD0E@gmail.com> Ada Feldman does a great course in fundamentals! I believe I have the power point from NSH last year if I can find it! Sent from my iPhone > On May 19, 2018, at 5:17 PM, Saundra Ellis via Histonet wrote: > > Anything written by Freida Carson. > > Saundra Ellis > Histology Supervisor > Florida Woman Care Laboratory > Cell (360) 513-9665 (best contact) > FAX (813) 433-5542 > > saundra.ellis at floridawomancare.com > > > ________________________________ > From: Gordon Brown via Histonet > Sent: Saturday, May 19, 2018 2:49:45 PM > To: histonet at lists.utsouthwestern.edu > Cc: Gordon Brown > Subject: [Histonet] Histopathology text recommendations > > Can anyone give me recommendations for a good histopathology text and atlas? I'm not a professional or even a formal student, I'm an amateur microscopist with a keen interest in histology, although I did attend a UK medical school many years ago only to decide that medicine was not for me. I'm now retired and expanding my interest in microscopy, which has fascinated me since the age of 8, I now have a collection of decent microscopes and microtomes and I'm setting up my study as a home microscopy and slide making facility. I have a growing collection of old histology slides including a number of pathology examples and I'm keen to be able to identify the histological changes that take place due to disease. In particular I am looking to be able to identify and photograph carcinomas as I recently acquired a photomicrography bench used by the Imperial Cancer Research Campaign in London during the 1950s and 1960s, and it would be appropriate to use it for the purpose it was designed for. > > Hope someone can help, I've subscribed to this list for many years but only rarely posted anything, the last time was when I enquired about replaceable blade holders for the original Cambridge rocking microtome and a very kind histologist in the USA sent me a slightly damaged holder and some blades. Fortunately I never managed to adapt it for the Cambridge as it is a perfect fit for the Reichert and Leitz rotary microtomes I now own! > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > CONFIDENTIALITY NOTICE: The information contained in this transmission may be privileged and confidential, as it is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this transmission in error, please immediately reply to the sender that you have received this communication in error and then delete it. Thank you. > CONFIDENTIALITY NOTICE: The information contained in this transmission may be privileged and confidential, as it is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this transmission in error, please immediately reply to the sender that you have received this communication in error and then delete it. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hans at histologistics.com Sun May 20 14:13:21 2018 From: hans at histologistics.com (Hans B Snyder) Date: Sun, 20 May 2018 15:13:21 -0400 Subject: [Histonet] tissue blocks available for banking Message-ID: Hello Curt, Will you give them to a private histology company like ours? We are constantly in need of human tissues for IHC/IF controls. If you will, we can give you our fedex or UPS account number. Thank you Hans B Snyder JMD Histology & Histologistics Inc. 151 W Main Street Dudley, MA 01571 Lab - 508-461-7207 C- 508-308-7800 www.jmdhistology.com? The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. On Sun, May 20, 2018 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. tissue blocks available for banking (Curt) > 2. histo-off topic (RAY KOELLING) > 3. Histopathology text recommendations (Gordon Brown) > 4. Re: Histopathology text recommendations (Saundra Ellis) > 5. Re: Histopathology text recommendations (Cristi Rigazio) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 19 May 2018 17:59:03 +0000 > From: Curt < > ?? > c.tague at Pathologyarts.com> > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] tissue blocks available for banking > Message-ID: > <9C8F910F72893643B3C3793C3D67132B68BDA9BA at PATHOLOGYSERVER. > pathologyarts.local> > > Content-Type: text/plain; charset="us-ascii" > > If anyone is looking for tissue for a bank, I have a boxes and boxes of > derm specimens and GI specimens. I know they're probably not what everyone > needs but if someone out there does need them, you can have them. figured > I'd ask before I disposed of them... at the landfill next to the city water > well... LOL, just kidding, I know someone out there will not think that's > funny, it's just a joke. > > > Curt > > > > > CONFIDENTIALITY NOTE: The information transmitted, including attachments, > is intended only for the person(s) or entity to which it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of, or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this in error, please > contact the sender and destroy any copies of this information. > > > ------------------------------ > > Message: 2 > Date: Sat, 19 May 2018 12:06:38 -0700 (PDT) > From: RAY KOELLING > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] histo-off topic > Message-ID: <578292945.1410691.1526756798387 at connect.xfinity.com> > Content-Type: text/plain; charset=UTF-8 > > Hello all, > > > > returned late last night from Pittsburg and the Intel-International > Science and Engineering Fair, May 13-18. The largest most prestigious > pre-college STEM fair in the world. Took four high school students from > the Spokane region. One of our other "Team Washington" students from near > Seattle won the second place prize for the entire 1,400 project fair from > 81 countries and regions. A $50,000 scholarship with a chemical > engineering project. > > > While there, I viewed or walked by every project. Saw 50-60 with > histology images/histology projects. IF, IHC, H&E, Safrinin O, FISH, PAS, > decals, frozen, TMA's; anything and everything we all know. On > osteoarthritis, breast cancer, kidney disease, diabetic pancreas, neuro, > lymphoma's and more. > > > If you are able to help what could be a potential world class student in > the field of histology/pathology/medical and clinical research, hope you > will. > > > Sorry I was never able to get to the local histology school while my > students were being judged in Convention Center. Couldn't seem to ever > contact them. > > > Ray Koelling > > > Eastern Washington Regional Science and Engineering Fair, Fair Director > > Washington State Science and Engineering Fair, Board Member > > lecture faculty, University of Washington School of Medicine, > Spokane-WWAMI site > > > ------------------------------ > > Message: 3 > Date: Sat, 19 May 2018 20:49:45 +0200 > From: Gordon Brown > To: histonet at lists.utsouthwestern.edu > Cc: Gordon Brown > Subject: [Histonet] Histopathology text recommendations > Message-ID: <4cf2q0p06jig4l65brthlbss.1526755785375 at email.android.com> > Content-Type: text/plain; charset=utf-8 > > Can anyone give me recommendations for a good histopathology text and > atlas? I'm not a professional or even a formal student, I'm an amateur > microscopist with a keen interest in histology, although I did attend a UK > medical school many years ago only to decide that medicine was not for me. > I'm now retired and expanding my interest in microscopy, which has > fascinated me since the age of 8, I now have a collection of decent > microscopes and microtomes and I'm setting up my study as a home microscopy > and slide making facility. I have a growing collection of old histology > slides including a number of pathology examples and I'm keen to be able to > identify the histological changes that take place due to disease. In > particular I am looking to be able to identify and photograph carcinomas as > I recently acquired a photomicrography bench used by the Imperial Cancer > Research Campaign in London during the 1950s and 1960s, and it would be > appropriate to use it for the purpose it was designed for. > > Hope someone can help, I've subscribed to this list for many years but > only rarely posted anything, the last time was when I enquired about > replaceable blade holders for the original Cambridge rocking microtome and > a very kind histologist in the USA sent me a slightly damaged holder and > some blades. Fortunately I never managed to adapt it for the Cambridge as > it is a perfect fit for the Reichert and Leitz rotary microtomes I now own! > > ------------------------------ > > Message: 4 > Date: Sat, 19 May 2018 21:17:25 +0000 > From: Saundra Ellis > To: Gordon Brown , > "histonet at lists.utsouthwestern.edu" > > Cc: Gordon Brown > Subject: Re: [Histonet] Histopathology text recommendations > Message-ID: > namprd19.prod.outlook.com> > > Content-Type: text/plain; charset="us-ascii" > > Anything written by Freida Carson. > > Saundra Ellis > Histology Supervisor > Florida Woman Care Laboratory > Cell (360) 513-9665 (best contact) > FAX (813) 433-5542 > > saundra.ellis at floridawomancare.com > > > ________________________________ > From: Gordon Brown via Histonet > Sent: Saturday, May 19, 2018 2:49:45 PM > To: histonet at lists.utsouthwestern.edu > Cc: Gordon Brown > Subject: [Histonet] Histopathology text recommendations > > Can anyone give me recommendations for a good histopathology text and > atlas? I'm not a professional or even a formal student, I'm an amateur > microscopist with a keen interest in histology, although I did attend a UK > medical school many years ago only to decide that medicine was not for me. > I'm now retired and expanding my interest in microscopy, which has > fascinated me since the age of 8, I now have a collection of decent > microscopes and microtomes and I'm setting up my study as a home microscopy > and slide making facility. I have a growing collection of old histology > slides including a number of pathology examples and I'm keen to be able to > identify the histological changes that take place due to disease. In > particular I am looking to be able to identify and photograph carcinomas as > I recently acquired a photomicrography bench used by the Imperial Cancer > Research Campaign in London during the 1950s and 1960s, and it would be > appropriate to use it for the purpose it was designed for. > > Hope someone can help, I've subscribed to this list for many years but > only rarely posted anything, the last time was when I enquired about > replaceable blade holders for the original Cambridge rocking microtome and > a very kind histologist in the USA sent me a slightly damaged holder and > some blades. Fortunately I never managed to adapt it for the Cambridge as > it is a perfect fit for the Reichert and Leitz rotary microtomes I now own! > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > CONFIDENTIALITY NOTICE: The information contained in this transmission may > be privileged and confidential, as it is intended only for the use of the > individual or entity named above. If the reader of this message is not the > intended recipient, you are hereby notified that any dissemination, > distribution or copying of this communication is strictly prohibited. If > you have received this transmission in error, please immediately reply to > the sender that you have received this communication in error and then > delete it. Thank you. > CONFIDENTIALITY NOTICE: The information contained in this transmission may > be privileged and confidential, as it is intended only for the use of the > individual or entity named above. If the reader of this message is not the > intended recipient, you are hereby notified that any dissemination, > distribution or copying of this communication is strictly prohibited. If > you have received this transmission in error, please immediately reply to > the sender that you have received this communication in error and then > delete it. Thank you. > > > ------------------------------ > > Message: 5 > Date: Sat, 19 May 2018 19:53:12 -0400 > From: Cristi Rigazio > To: Saundra Ellis > Cc: Gordon Brown , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Histopathology text recommendations > Message-ID: <37037DD4-E107-4CA7-A4DE-6C4D013BCD0E at gmail.com> > Content-Type: text/plain; charset=us-ascii > > Ada Feldman does a great course in fundamentals! I believe I have the > power point from NSH last year if I can find it! > > Sent from my iPhone > > > On May 19, 2018, at 5:17 PM, Saundra Ellis via Histonet utsouthwestern.edu> wrote: > > > > Anything written by Freida Carson. > > > > Saundra Ellis > > Histology Supervisor > > Florida Woman Care Laboratory > > Cell (360) 513-9665 (best contact) > > FAX (813) 433-5542 > > > > saundra.ellis at floridawomancare.com > > > > > > ________________________________ > > From: Gordon Brown via Histonet > > Sent: Saturday, May 19, 2018 2:49:45 PM > > To: histonet at lists.utsouthwestern.edu > > Cc: Gordon Brown > > Subject: [Histonet] Histopathology text recommendations > > > > Can anyone give me recommendations for a good histopathology text and > atlas? I'm not a professional or even a formal student, I'm an amateur > microscopist with a keen interest in histology, although I did attend a UK > medical school many years ago only to decide that medicine was not for me. > I'm now retired and expanding my interest in microscopy, which has > fascinated me since the age of 8, I now have a collection of decent > microscopes and microtomes and I'm setting up my study as a home microscopy > and slide making facility. I have a growing collection of old histology > slides including a number of pathology examples and I'm keen to be able to > identify the histological changes that take place due to disease. In > particular I am looking to be able to identify and photograph carcinomas as > I recently acquired a photomicrography bench used by the Imperial Cancer > Research Campaign in London during the 1950s and 1960s, and it would be > appropriate to use it for the purpose it was designed for. > > > > Hope someone can help, I've subscribed to this list for many years but > only rarely posted anything, the last time was when I enquired about > replaceable blade holders for the original Cambridge rocking microtome and > a very kind histologist in the USA sent me a slightly damaged holder and > some blades. Fortunately I never managed to adapt it for the Cambridge as > it is a perfect fit for the Reichert and Leitz rotary microtomes I now own! > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: The information contained in this transmission > may be privileged and confidential, as it is intended only for the use of > the individual or entity named above. If the reader of this message is not > the intended recipient, you are hereby notified that any dissemination, > distribution or copying of this communication is strictly prohibited. If > you have received this transmission in error, please immediately reply to > the sender that you have received this communication in error and then > delete it. Thank you. > > CONFIDENTIALITY NOTICE: The information contained in this transmission > may be privileged and confidential, as it is intended only for the use of > the individual or entity named above. If the reader of this message is not > the intended recipient, you are hereby notified that any dissemination, > distribution or copying of this communication is strictly prohibited. If > you have received this transmission in error, please immediately reply to > the sender that you have received this communication in error and then > delete it. Thank you. > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 174, Issue 17 > ***************************************** > From john.garratt at ciqc.ca Sun May 20 15:02:45 2018 From: john.garratt at ciqc.ca (john.garratt at ciqc.ca) Date: Sun, 20 May 2018 13:02:45 -0700 Subject: [Histonet] IHC Symposium in Quebec City Message-ID: <000001d3f075$84f22d20$8ed68760$@ciqc.ca> 2018 cIQc Symposium: IHC Toolkits for Diagnostic Pathology Where: Quebec City at the Hotel Palace Royale (775 Boulevard Honor?-Mercier) When: September 20-21, 2018. Go to www.ciqc.ca for more information. The symposium is for Lab Scientists, Pathologists and Industry to discuss a variety of topics including PD-L1, Alk, Ros1 John Garratt ? Canadian Immunohistochemistry Quality Control From john.frazier at roche.com Sun May 20 16:31:01 2018 From: john.frazier at roche.com (Frazier, John) Date: Sun, 20 May 2018 17:31:01 -0400 Subject: [Histonet] Histopathology text recommendations Message-ID: Histotechnology: A Self-Instructional Text Sent from my iPhone On May 19, 2018, at 5:17 PM, Saundra Ellis < saundra.ellis at floridawomancare.com> wrote: Anything written by Freida Carson. Saundra Ellis Histology Supervisor Florida Woman Care Laboratory Cell (360) 513-9665 (best contact) FAX (813) 433-5542 saundra.ellis at floridawomancare.com ________________________________ From: Gordon Brown via Histonet Sent: Saturday, May 19, 2018 2:49:45 PM To: histonet at lists.utsouthwestern.edu Cc: Gordon Brown Subject: [Histonet] Histopathology text recommendations Can anyone give me recommendations for a good histopathology text and atlas? I'm not a professional or even a formal student, I'm an amateur microscopist with a keen interest in histology, although I did attend a UK medical school many years ago only to decide that medicine was not for me. I'm now retired and expanding my interest in microscopy, which has fascinated me since the age of 8, I now have a collection of decent microscopes and microtomes and I'm setting up my study as a home microscopy and slide making facility. I have a growing collection of old histology slides including a number of pathology examples and I'm keen to be able to identify the histological changes that take place due to disease. In particular I am looking to be able to identify and photograph carcinomas as I recently acquired a photomicrography bench used by the Imperial Cancer Research Campaign in London during the 1950s and 1960s, and it would be appropriate to use it for the purpose it was designed for. Hope someone can help, I've subscribed to this list for many years but only rarely posted anything, the last time was when I enquired about replaceable blade holders for the original Cambridge rocking microtome and a very kind histologist in the USA sent me a slightly damaged holder and some blades. Fortunately I never managed to adapt it for the Cambridge as it is a perfect fit for the Reichert and Leitz rotary microtomes I now own! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this transmission may be privileged and confidential, as it is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this transmission in error, please immediately reply to the sender that you have received this communication in error and then delete it. Thank you. CONFIDENTIALITY NOTICE: The information contained in this transmission may be privileged and confidential, as it is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this transmission in error, please immediately reply to the sender that you have received this communication in error and then delete it. Thank you. From badams at acadianagastro.com Mon May 21 08:53:57 2018 From: badams at acadianagastro.com (Brent Adams) Date: Mon, 21 May 2018 13:53:57 +0000 Subject: [Histonet] Histonet Digest, Vol 174, Issue 17. "Tissue blocks available for banking" In-Reply-To: References: Message-ID: Curt I could use some positive H-pylori stomach tissue for HP controls and would take others GI for H&E controls. Let me know how to make contact and we can set up arrangements to ship blocks here. Thanks Brent Adams ? BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Sunday, May 20, 2018 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 174, Issue 17 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. tissue blocks available for banking (Curt) 2. histo-off topic (RAY KOELLING) 3. Histopathology text recommendations (Gordon Brown) 4. Re: Histopathology text recommendations (Saundra Ellis) 5. Re: Histopathology text recommendations (Cristi Rigazio) ---------------------------------------------------------------------- Message: 1 Date: Sat, 19 May 2018 17:59:03 +0000 From: Curt To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] tissue blocks available for banking Message-ID: <9C8F910F72893643B3C3793C3D67132B68BDA9BA at PATHOLOGYSERVER.pathologyarts.local> Content-Type: text/plain; charset="us-ascii" If anyone is looking for tissue for a bank, I have a boxes and boxes of derm specimens and GI specimens. I know they're probably not what everyone needs but if someone out there does need them, you can have them. figured I'd ask before I disposed of them... at the landfill next to the city water well... LOL, just kidding, I know someone out there will not think that's funny, it's just a joke. Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. ------------------------------ Message: 2 Date: Sat, 19 May 2018 12:06:38 -0700 (PDT) From: RAY KOELLING To: histonet at lists.utsouthwestern.edu Subject: [Histonet] histo-off topic Message-ID: <578292945.1410691.1526756798387 at connect.xfinity.com> Content-Type: text/plain; charset=UTF-8 Hello all, returned late last night from Pittsburg and the Intel-International Science and Engineering Fair, May 13-18. The largest most prestigious pre-college STEM fair in the world. Took four high school students from the Spokane region. One of our other "Team Washington" students from near Seattle won the second place prize for the entire 1,400 project fair from 81 countries and regions. A $50,000 scholarship with a chemical engineering project. While there, I viewed or walked by every project. Saw 50-60 with histology images/histology projects. IF, IHC, H&E, Safrinin O, FISH, PAS, decals, frozen, TMA's; anything and everything we all know. On osteoarthritis, breast cancer, kidney disease, diabetic pancreas, neuro, lymphoma's and more. If you are able to help what could be a potential world class student in the field of histology/pathology/medical and clinical research, hope you will. Sorry I was never able to get to the local histology school while my students were being judged in Convention Center. Couldn't seem to ever contact them. Ray Koelling Eastern Washington Regional Science and Engineering Fair, Fair Director Washington State Science and Engineering Fair, Board Member lecture faculty, University of Washington School of Medicine, Spokane-WWAMI site ------------------------------ Message: 3 Date: Sat, 19 May 2018 20:49:45 +0200 From: Gordon Brown To: histonet at lists.utsouthwestern.edu Cc: Gordon Brown Subject: [Histonet] Histopathology text recommendations Message-ID: <4cf2q0p06jig4l65brthlbss.1526755785375 at email.android.com> Content-Type: text/plain; charset=utf-8 Can anyone give me recommendations for a good histopathology text and atlas? I'm not a professional or even a formal student, I'm an amateur microscopist with a keen interest in histology, although I did attend a UK medical school many years ago only to decide that medicine was not for me. I'm now retired and expanding my interest in microscopy, which has fascinated me since the age of 8, I now have a collection of decent microscopes and microtomes and I'm setting up my study as a home microscopy and slide making facility. I have a growing collection of old histology slides including a number of pathology examples and I'm keen to be able to identify the histological changes that take place due to disease. In particular I am looking to be able to identify and photograph carcinomas as I recently acquired a photomicrography bench used by the Imperial Cancer Research Campaign in London during the 1950s and 1960s, and it would be appropriate to use it for the purpose it was designed for. Hope someone can help, I've subscribed to this list for many years but only rarely posted anything, the last time was when I enquired about replaceable blade holders for the original Cambridge rocking microtome and a very kind histologist in the USA sent me a slightly damaged holder and some blades. Fortunately I never managed to adapt it for the Cambridge as it is a perfect fit for the Reichert and Leitz rotary microtomes I now own! ------------------------------ Message: 4 Date: Sat, 19 May 2018 21:17:25 +0000 From: Saundra Ellis To: Gordon Brown , "histonet at lists.utsouthwestern.edu" Cc: Gordon Brown Subject: Re: [Histonet] Histopathology text recommendations Message-ID: Content-Type: text/plain; charset="us-ascii" Anything written by Freida Carson. Saundra Ellis Histology Supervisor Florida Woman Care Laboratory Cell (360) 513-9665 (best contact) FAX (813) 433-5542 saundra.ellis at floridawomancare.com ________________________________ From: Gordon Brown via Histonet Sent: Saturday, May 19, 2018 2:49:45 PM To: histonet at lists.utsouthwestern.edu Cc: Gordon Brown Subject: [Histonet] Histopathology text recommendations Can anyone give me recommendations for a good histopathology text and atlas? I'm not a professional or even a formal student, I'm an amateur microscopist with a keen interest in histology, although I did attend a UK medical school many years ago only to decide that medicine was not for me. I'm now retired and expanding my interest in microscopy, which has fascinated me since the age of 8, I now have a collection of decent microscopes and microtomes and I'm setting up my study as a home microscopy and slide making facility. I have a growing collection of old histology slides including a number of pathology examples and I'm keen to be able to identify the histological changes that take place due to disease. In particular I am looking to be able to identify and photograph carcinomas as I recently acquired a photomicrography bench used by the Imperial Cancer Research Campaign in London during the 1950s and 1960s, and it would be appropriate to use it for the purpose it was designed for. Hope someone can help, I've subscribed to this list for many years but only rarely posted anything, the last time was when I enquired about replaceable blade holders for the original Cambridge rocking microtome and a very kind histologist in the USA sent me a slightly damaged holder and some blades. Fortunately I never managed to adapt it for the Cambridge as it is a perfect fit for the Reichert and Leitz rotary microtomes I now own! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this transmission may be privileged and confidential, as it is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this transmission in error, please immediately reply to the sender that you have received this communication in error and then delete it. Thank you. CONFIDENTIALITY NOTICE: The information contained in this transmission may be privileged and confidential, as it is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this transmission in error, please immediately reply to the sender that you have received this communication in error and then delete it. Thank you. ------------------------------ Message: 5 Date: Sat, 19 May 2018 19:53:12 -0400 From: Cristi Rigazio To: Saundra Ellis Cc: Gordon Brown , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Histopathology text recommendations Message-ID: <37037DD4-E107-4CA7-A4DE-6C4D013BCD0E at gmail.com> Content-Type: text/plain; charset=us-ascii Ada Feldman does a great course in fundamentals! I believe I have the power point from NSH last year if I can find it! Sent from my iPhone > On May 19, 2018, at 5:17 PM, Saundra Ellis via Histonet wrote: > > Anything written by Freida Carson. > > Saundra Ellis > Histology Supervisor > Florida Woman Care Laboratory > Cell (360) 513-9665 (best contact) > FAX (813) 433-5542 > > saundra.ellis at floridawomancare.com > > > ________________________________ > From: Gordon Brown via Histonet > Sent: Saturday, May 19, 2018 2:49:45 PM > To: histonet at lists.utsouthwestern.edu > Cc: Gordon Brown > Subject: [Histonet] Histopathology text recommendations > > Can anyone give me recommendations for a good histopathology text and atlas? I'm not a professional or even a formal student, I'm an amateur microscopist with a keen interest in histology, although I did attend a UK medical school many years ago only to decide that medicine was not for me. I'm now retired and expanding my interest in microscopy, which has fascinated me since the age of 8, I now have a collection of decent microscopes and microtomes and I'm setting up my study as a home microscopy and slide making facility. I have a growing collection of old histology slides including a number of pathology examples and I'm keen to be able to identify the histological changes that take place due to disease. In particular I am looking to be able to identify and photograph carcinomas as I recently acquired a photomicrography bench used by the Imperial Cancer Research Campaign in London during the 1950s and 1960s, and it would be appropriate to use it for the purpose it was designed for. > > Hope someone can help, I've subscribed to this list for many years but only rarely posted anything, the last time was when I enquired about replaceable blade holders for the original Cambridge rocking microtome and a very kind histologist in the USA sent me a slightly damaged holder and some blades. Fortunately I never managed to adapt it for the Cambridge as it is a perfect fit for the Reichert and Leitz rotary microtomes I now own! > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > CONFIDENTIALITY NOTICE: The information contained in this transmission may be privileged and confidential, as it is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this transmission in error, please immediately reply to the sender that you have received this communication in error and then delete it. Thank you. > CONFIDENTIALITY NOTICE: The information contained in this transmission may be privileged and confidential, as it is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this transmission in error, please immediately reply to the sender that you have received this communication in error and then delete it. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 174, Issue 17 ***************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. From criley at dpspa.com Mon May 21 09:50:49 2018 From: criley at dpspa.com (Charles Riley) Date: Mon, 21 May 2018 10:50:49 -0400 Subject: [Histonet] IHC question Message-ID: Does anyone run this antibody on their Leica bond platforms? BRAF V600E (VE1)? I am looing to purchase it from sigma aldrich From RCazares at schosp.org Mon May 21 10:33:20 2018 From: RCazares at schosp.org (Cazares, Ruth) Date: Mon, 21 May 2018 10:33:20 -0500 Subject: [Histonet] histology temporary help Message-ID: Hi all, I am looking for temporary help for the summer, 13 weeks from end of July to the end of October. Early morning 6 am to 2:30 (or 5am to 1:30 pm). If anyone is interested or knows someone, please have them fill out the online application for Swedish Covenant Hospital (Chicago). OR... Does anyone know of a temp agency that staffs Histotechs and is local? Thanks in advance!! Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 N. California Chicago, IL 60625 (773) 878-8200 ext-5190 From thisisann at aol.com Mon May 21 14:02:28 2018 From: thisisann at aol.com (Ann Specian) Date: Mon, 21 May 2018 15:02:28 -0400 Subject: [Histonet] Billing for iuds with tissue Message-ID: <1638414d8cc-c91-38e1@webjas-vae192.srv.aolmail.net> Can someone tell me the correct way to bill an IUD that has tissue. If there is no tissue then we just bill cpt code 88300. If there is tissue should you bill 88305 ........or 88300 and 88305? From epeters2 at gmu.edu Mon May 21 18:09:15 2018 From: epeters2 at gmu.edu (Esther C Peters) Date: Mon, 21 May 2018 23:09:15 +0000 Subject: [Histonet] Histopathology text recommendations In-Reply-To: References: Message-ID: Hi Gordon, I think you meant a histopathology atlas for slide reading, right? I am not a human histopathologist, but there are many such texts available, for example, see these at Amazon: https://www.amazon.com/s/ref=nb_sb_noss_1?url=search-alias%3Dstripbooks&field-keywords=histopathology Amazon.com: histopathology: Books www.amazon.com Online shopping from a great selection at Books Store. Also, several websites from medical schools offer virtual microscopy histoslide collections of different pathologies, such as: https://med.virginia.edu/biomolecular-analysis-facility/services/shared-instrumentation/aperio-scanscope-slide-scanner/catalogs-of-histology-and-pathology-virtual-slides-on-the-web/ http://www.mbfbioscience.com/iowavirtualslidebox https://www.amboss.com/us/knowledge/Virtual_histopathology_slide_box And others, just search in Google for "histopathology virtual slide box." Perhaps one of the pathologists on this list-serv can guide you to the best one(s) for your needs! Esther Esther C. Peters, Ph.D. Term Associate Professor Environmental Science & Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030-4444 Office: David King Hall, Room 3050 Phone: 703-993-3462 Fax: 703-993-1066 e-mail: epeters2 at gmu.edu http://esp.gmu.edu ________________________________ From: Frazier, John via Histonet Sent: Sunday, May 20, 2018 5:31:01 PM To: Saundra Ellis Cc: histonet at lists.utsouthwestern.edu; Gordon Brown Subject: Re: [Histonet] Histopathology text recommendations Histotechnology: A Self-Instructional Text Sent from my iPhone On May 19, 2018, at 5:17 PM, Saundra Ellis < saundra.ellis at floridawomancare.com> wrote: Anything written by Freida Carson. Saundra Ellis Histology Supervisor Florida Woman Care Laboratory Cell (360) 513-9665 (best contact) FAX (813) 433-5542 saundra.ellis at floridawomancare.com ________________________________ From: Gordon Brown via Histonet Sent: Saturday, May 19, 2018 2:49:45 PM To: histonet at lists.utsouthwestern.edu Cc: Gordon Brown Subject: [Histonet] Histopathology text recommendations Can anyone give me recommendations for a good histopathology text and atlas? I'm not a professional or even a formal student, I'm an amateur microscopist with a keen interest in histology, although I did attend a UK medical school many years ago only to decide that medicine was not for me. I'm now retired and expanding my interest in microscopy, which has fascinated me since the age of 8, I now have a collection of decent microscopes and microtomes and I'm setting up my study as a home microscopy and slide making facility. I have a growing collection of old histology slides including a number of pathology examples and I'm keen to be able to identify the histological changes that take place due to disease. In particular I am looking to be able to identify and photograph carcinomas as I recently acquired a photomicrography bench used by the Imperial Cancer Research Campaign in London during the 1950s and 1960s, and it would be appropriate to use it for the purpose it was designed for. Hope someone can help, I've subscribed to this list for many years but only rarely posted anything, the last time was when I enquired about replaceable blade holders for the original Cambridge rocking microtome and a very kind histologist in the USA sent me a slightly damaged holder and some blades. Fortunately I never managed to adapt it for the Cambridge as it is a perfect fit for the Reichert and Leitz rotary microtomes I now own! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1GfcR_zVGSNZFprqljlK8MjuhxAYpfP_QQTByrhqt0Kr-8g_1tLjUE7U975eLICUinF2QuqNDTYEwySGvoO3MckIR7aPYIJWxWkfFU6PgoXls4px4v4M9ieidWRc2UKuyT_gFjkyGmhTAblsJg3p32hE-rwF5K0PHxrDz5Cg4bSDcrSvJZ8rkhqIExA4chiqHX9idd4CO3tF8ntTCKBPyR-WFdT-cjTfdI6JQfhohSMGkRty97ApMwmkp0rzHciE8aTB1k5jF8zh5zPogwnhPms9QMzen_fTLkSlXDcgeHqU__Se8UMaq0j1d1y1R540__zWnWuRd7Wrd55uLx4PyD7cE-KUdjirYuAHP4ymzLKMmf3h04IqBiGjHSIx3aLZDE4hlSsjEgYb5pldCRRZb7ZLEf-DHCk7Oqke-a_gRT2uERiFGVBXnweu_o5XU96pd/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: The information contained in this transmission may be privileged and confidential, as it is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this transmission in error, please immediately reply to the sender that you have received this communication in error and then delete it. Thank you. CONFIDENTIALITY NOTICE: The information contained in this transmission may be privileged and confidential, as it is intended only for the use of the individual or entity named above. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this transmission in error, please immediately reply to the sender that you have received this communication in error and then delete it. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1GfcR_zVGSNZFprqljlK8MjuhxAYpfP_QQTByrhqt0Kr-8g_1tLjUE7U975eLICUinF2QuqNDTYEwySGvoO3MckIR7aPYIJWxWkfFU6PgoXls4px4v4M9ieidWRc2UKuyT_gFjkyGmhTAblsJg3p32hE-rwF5K0PHxrDz5Cg4bSDcrSvJZ8rkhqIExA4chiqHX9idd4CO3tF8ntTCKBPyR-WFdT-cjTfdI6JQfhohSMGkRty97ApMwmkp0rzHciE8aTB1k5jF8zh5zPogwnhPms9QMzen_fTLkSlXDcgeHqU__Se8UMaq0j1d1y1R540__zWnWuRd7Wrd55uLx4PyD7cE-KUdjirYuAHP4ymzLKMmf3h04IqBiGjHSIx3aLZDE4hlSsjEgYb5pldCRRZb7ZLEf-DHCk7Oqke-a_gRT2uERiFGVBXnweu_o5XU96pd/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet From Valerie.Cortright at uvmhealth.org Tue May 22 14:01:25 2018 From: Valerie.Cortright at uvmhealth.org (Cortright, Valerie) Date: Tue, 22 May 2018 19:01:25 +0000 Subject: [Histonet] We're Hiring for Histotechnologist! Message-ID: The University of Vermont Medical Center is hiring for a HT or HTL https://uvmhealth.wd1.myworkdayjobs.com/EXTERNAL/job/Burlington-VT/Histotechnologist_R0009206?shared_id=7cbe9a9a-ef11-4705-ade2-255137deb068 Feel free to share this! This message and any attachments may contain information that is confidential, privileged and/or protected from disclosure under state and federal laws. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. From emcca2 at gmail.com Tue May 22 17:26:07 2018 From: emcca2 at gmail.com (Erin McCarthy) Date: Tue, 22 May 2018 17:26:07 -0500 Subject: [Histonet] Molecular Testing Message-ID: Hi All, When cutting for NGS testing what additional measures do you take to minimize contamination? We are a pretty clean outfit here, but still, have some trace sample carryover occurring in cases we cut in-house. We already use a fresh blade with every case, use tools like forceps instead of fingers to transfer sections from our blade to our bath, and wipe down the blade holder and water bath surface between every case. Our NGS team is finding very small - like flake sized bits - of contamination in samples, we do not know for sure that it is from cutting. However, they want all departments to troubleshoot. Any suggestions would be a great help! From Karen.Heckford at DignityHealth.org Wed May 23 06:46:38 2018 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Wed, 23 May 2018 11:46:38 +0000 Subject: [Histonet] TMA slides Message-ID: <0075a13375c7490ca5fb8ef20d79c0cf@PHX-EXCH-013.chw.edu> Good Morning, Does anyone know where I can purchase some CMV TMA slides? Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you From relia1 at earthlink.net Wed May 23 13:47:09 2018 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 23 May 2018 14:47:09 -0400 Subject: [Histonet] Mohs Histotech Needed in Philadelphia A RELIA EXCLUSIVE CAN YOU HELP??? Message-ID: <063401d3f2c6$c7d3f6c0$577be440$@earthlink.net> Hi Histonetters, How are you? I hope you are having a great week! I was hoping you might be able to help me. I am presently working with a client near Philadelphia that is in need of a Mohs histotech to run their own lab. This is a full time permanent DAY shift position. ASCP certification is preferred and 2 years of experience and CLIA qualifications to gross are required. Histonetters, the position involves performing routine histology, grossing and Mohs. The help I need from you is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them? (remember if I place someone you refer to me you will earn a 250.00 referral fee.) This is a RELIA Exclusive and my client offers a competitive compensation package and a great place to work!!!! If you are interested in this position please contact me ASAP toll free at 866-607-3542, cell/text 407-353-5070 or via email at relia1 at earthlink.net If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won't release it to anyone without your permission. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Susan.Dachel at va.gov Thu May 24 09:21:21 2018 From: Susan.Dachel at va.gov (Dachel, Susan K.) Date: Thu, 24 May 2018 14:21:21 +0000 Subject: [Histonet] Molecular Work Load Credit for Histology Message-ID: Is there a CPT code to capture workload credit for the slide or tube prep on FFPE sections prepared by histology and forwarded to Molecular for testing? Sue Dachel BA, HTL(ASCP)CM Histology Supervisor Minneapolis VA Health Care System From tkngflght at yahoo.com Thu May 24 13:43:30 2018 From: tkngflght at yahoo.com (Cheryl) Date: Thu, 24 May 2018 18:43:30 +0000 (UTC) Subject: [Histonet] procedure for peripheral nerve damage References: <1116479195.865595.1527187410435.ref@mail.yahoo.com> Message-ID: <1116479195.865595.1527187410435@mail.yahoo.com> A client wants us to start reading peripheral neuropathy biopsies.? ?Hoping I don't have to start from scratch - anyone have processing and prep procedures including cutting protocols? Much appreciated!!?Cheryl Kerry, HT(ASCP)? Houston TX? From Timothy.Morken at ucsf.edu Thu May 24 13:48:22 2018 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 24 May 2018 18:48:22 +0000 Subject: [Histonet] Warm up freezer-stored powder, or use right away? Message-ID: We have a debate going on for those freezer-stored powdered chemicals used for enzyme histochemistry. One side says warm up the container to room temperature before opening in order to prevent water condensation in the container/powder. The other side says to use right away and put back in the freezer to avoid long exposure at room temperature. I'd like to hear pros and cons of each and any references stating one practice or the other. Thanks! Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From jriggleman at globusmedical.com Thu May 24 14:01:56 2018 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Thu, 24 May 2018 19:01:56 +0000 Subject: [Histonet] Umbilical Cord Embedding Message-ID: Hello, Does anyone have any experience embedding umbilical cord tissue? Preferably paraffin but we may try plastic as well. Any help/protocols/etc. would be appreciated. Thank You, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From melissa at alliedsearchpartners.com Thu May 24 14:01:57 2018 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Thu, 24 May 2018 19:01:57 +0000 Subject: [Histonet] Position Won't Last-Histotech in N Central FL- Message-ID: Hello All, I hope you are doing well and looking forward to the holiday weekend. I have a Full Time/Permanent position with an excellent client of mine in Northern/Central Florida. It's M-F 1pm-9:30pm. This position comes with excellent pay, benefits, management staff and working environment. This also offers relocation assistance. Must be FL Histology Technologist licensed or eligible if out of state. For immediate consideration and to streamline your resume directly to hiring manager, please reach out to me with your resume. This position won't last! melissa at alliedsearchpartners.com or fax: 888-388-7572 Melissa Owens, CHP (ASA) President, Laboratory Staffing From CDavis at che-east.org Thu May 24 14:07:57 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Thu, 24 May 2018 19:07:57 +0000 Subject: [Histonet] sample contamination Message-ID: Response to NGS contamination: Hi Erin, Each point in Histology has a risk area and points of contamination prevention: Grossing- wash gloves, forceps and specimen sampling area between specimens Embedding- Wipe forceps, fingers or gloves if you wear them while embedding and embedding surface with guaze between cassettes Microtome- Wipe forceps, fingers or gloves if you wear them during mictotomy with guaze between blocks and waterbath with kimwipes. If it is suggested the contamination is from the microtomist, maybe try microtomy with gloves if it is not currently being done that way. That's the only other thing I can think of. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From michael.gudo at morphisto.de Thu May 24 14:58:36 2018 From: michael.gudo at morphisto.de (Michael Gudo (Morphisto GmbH)) Date: Thu, 24 May 2018 21:58:36 +0200 Subject: [Histonet] Umbilical Cord Embedding In-Reply-To: References: Message-ID: Dear Jessica, we have done embedding in paraffine and the results are quite good. The critical point is fixation. Due to the high proportion of water in the tissue, the best fixation is possible with Bouin-solution or - if you want to do IHC stainings, Zamboni-fixation (which is a variant of Bouin with a buffered pH value). With trichromatic or polychromatic stainings you will get very colorful results. Best regards Michael > Am 24.05.2018 um 21:01 schrieb Jessica Riggleman via Histonet : > > Hello, > Does anyone have any experience embedding umbilical cord tissue? Preferably paraffin but we may try plastic as well. Any help/protocols/etc. would be appreciated. > > Thank You, > Jessica > > > _____________________________________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > > Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From michael.gudo at morphisto.de Fri May 25 00:19:36 2018 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Fri, 25 May 2018 07:19:36 +0200 Subject: [Histonet] Umbilical Cord Embedding In-Reply-To: References: Message-ID: Dear Jessica, fixation should be at least 48 hours, however it is not problematic if the tissues stay longer in these solutions. Important point is, to remove the picric acid, which is a component of the Bouin solution, with several steps of 70 - 80 % of ethanol, so after fixation go directly into 70 - 80 % ethanol, and change the ethanol several times, until the ethanols stay clear. To improve this process, you can add a few drops of ammoniak. Best regards Michael > Am 24.05.2018 um 23:49 schrieb Jessica Riggleman : > > Michael, > Thank you so much. How long do you live it in the fixation solution/alcohol/etc for typically? > > Thank You, > Jessica > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > An ISO 13485 Registered Company > > website | map | email > > Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. > From: Michael Gudo (Morphisto GmbH) [mailto:michael.gudo at morphisto.de ] > Sent: Thursday, May 24, 2018 3:59 PM > To: Jessica Riggleman > > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Umbilical Cord Embedding > > Dear Jessica, > > we have done embedding in paraffine and the results are quite good. The critical point is fixation. Due to the high proportion of water in the tissue, the best fixation is possible with Bouin-solution or - if you want to do IHC stainings, Zamboni-fixation (which is a variant of Bouin with a buffered pH value). > > With trichromatic or polychromatic stainings you will get very colorful results. > > Best regards > Michael > > > > Am 24.05.2018 um 21:01 schrieb Jessica Riggleman via Histonet >: > > Hello, > Does anyone have any experience embedding umbilical cord tissue? Preferably paraffin but we may try plastic as well. Any help/protocols/etc. would be appreciated. > > Thank You, > Jessica > > > _____________________________________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > > Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ************************************************************************************************ > MORPHISTO GmbH > PD Dr. phil. nat. Michael Gudo > Weism?llerstr. 45 > 60314 Frankfurt am Main > Telefon: 069 / 400 3019 - 62 > Telefax: 069 / 400 3019 - 64 > > E-Mail: michael.gudo at morphisto.de > Internet: http://www.morphisto.de/ > > Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo > > Registergericht: Amtsgericht Frankfurt > Registernummer: HRB 74954 > Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 > ************************************************************************************************ > Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. > ************************************************************************************************ > This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. ************************************************************************************************ MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From Samira.Alminawi at sunnybrook.ca Fri May 25 09:28:51 2018 From: Samira.Alminawi at sunnybrook.ca (Alminawi, Samira) Date: Fri, 25 May 2018 14:28:51 +0000 Subject: [Histonet] LEF1 - antibody Message-ID: <5c0dc4a9e8364573a7a9511949c2f624@SBXNG3.sw.ca> Hi, Is anybody using LEF1 antibody and what is the recommended protocol? Thank you for help Samira Alminawi, MLT Senior- Immunohistochemistry Sunnybrook Health Sciences Centre 2075 Bayview Avenue, Toronto, ON M4N 3M5 Tel: 416-480-6100 ext:1666 e-mail: samira.alminawi at sunnybrook.ca This e-mail is intended only for the named recipient(s) and may contain confidential, personal and/or health information (information which may be subject to legal restrictions on use, retention and/or disclosure). No waiver of confidence is intended by virtue of communication via the internet. Any review or distribution by anyone other than the person(s) for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and destroy all copies. From Richard.Cartun at hhchealth.org Fri May 25 09:50:13 2018 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 25 May 2018 14:50:13 +0000 Subject: [Histonet] LEF1 - antibody In-Reply-To: <5c0dc4a9e8364573a7a9511949c2f624@SBXNG3.sw.ca> References: <5c0dc4a9e8364573a7a9511949c2f624@SBXNG3.sw.ca> Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EA8A293FC@HHCEXCHMB03.hhcsystem.org> We use a RTU (clone EP310) from Cell Marque on the Leica Bond. Antigen retrieval is "high pH" HIER for 20 minutes. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -----Original Message----- From: Alminawi, Samira via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, May 25, 2018 10:29 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] LEF1 - antibody This email is from outside HHC. BE CAREFUL when opening attachments or links from unknown senders. Hi, Is anybody using LEF1 antibody and what is the recommended protocol? Thank you for help Samira Alminawi, MLT Senior- Immunohistochemistry Sunnybrook Health Sciences Centre 2075 Bayview Avenue, Toronto, ON M4N 3M5 Tel: 416-480-6100 ext:1666 e-mail: samira.alminawi at sunnybrook.ca This e-mail is intended only for the named recipient(s) and may contain confidential, personal and/or health information (information which may be subject to legal restrictions on use, retention and/or disclosure). No waiver of confidence is intended by virtue of communication via the internet. Any review or distribution by anyone other than the person(s) for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and destroy all copies. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Reminder: This e-mail and any attachments are subject to the current HHC email retention policies. Please save or store appropriately in accordance with policy. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Samira.Alminawi at sunnybrook.ca Fri May 25 09:56:25 2018 From: Samira.Alminawi at sunnybrook.ca (Alminawi, Samira) Date: Fri, 25 May 2018 14:56:25 +0000 Subject: [Histonet] LEF1 - antibody Message-ID: I tried LEF1 (EPR2029Y) from abcam and I couldn't get a good result. I worked this On Dako-Omnis instrument-H30x30 1/1200 dilution. Still not happy with the result. Samira From: Alminawi, Samira Sent: Friday, May 25, 2018 10:29 AM To: 'Histonet at lists.utsouthwestern.edu' Subject: LEF1 - antibody Hi, Is anybody using LEF1 antibody and what is the recommended protocol? Thank you for help Samira Alminawi, MLT Senior- Immunohistochemistry Sunnybrook Health Sciences Centre 2075 Bayview Avenue, Toronto, ON M4N 3M5 Tel: 416-480-6100 ext:1666 e-mail: samira.alminawi at sunnybrook.ca This e-mail is intended only for the named recipient(s) and may contain confidential, personal and/or health information (information which may be subject to legal restrictions on use, retention and/or disclosure). No waiver of confidence is intended by virtue of communication via the internet. Any review or distribution by anyone other than the person(s) for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and destroy all copies. From mtighe at trudeauinstitute.org Fri May 25 10:56:12 2018 From: mtighe at trudeauinstitute.org (Mike Tighe) Date: Fri, 25 May 2018 15:56:12 +0000 Subject: [Histonet] Fluorescent assay for Eosinophils Message-ID: Hi Everyone, I am looking for a specific marker for eosinophils. Came across some testing laboratories that offer Immunofluorescent assay for eMBP1 and EDN. Does anybody know of a good antibody source to label these components? Is this assay antibody based or dye based? Thanks! Mike From rsrichmond at gmail.com Fri May 25 12:39:24 2018 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 25 May 2018 13:39:24 -0400 Subject: [Histonet] procedure for peripheral nerve damage Message-ID: Cheryl Kerry, HT(ASCP) in Houston TX asks: >>A client wants us to start reading peripheral neuropathy biopsies. Hoping I don't have to start from scratch - anyone have processing and prep procedures including cutting protocols?<< This is a highly complicated procedure, often involving specialized dissection techniques ("teasing") and electron microscopy. Peripheral nerve biopsies are best left to experts - there are just a few labs that do them - Mayo Medical Laboratories is the only one I know of. You need to review this subject yourself, and then review it with your client. Bob Richmond Samurai Pathologist Maryville TN From rsrichmond at gmail.com Fri May 25 12:48:16 2018 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 25 May 2018 13:48:16 -0400 Subject: [Histonet] Warm up freezer-stored powder, or use right away? Message-ID: Tim Morken (Pathology Site Manager, Parnassus - Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology - UC San Francisco Medical Center) asks: >>We have a debate going on for those freezer-stored powdered chemicals used for enzyme histochemistry. One side says warm up the container to room temperature before opening in order to prevent water condensation in the container/powder. The other side says to use right away and put back in the freezer to avoid long exposure at room temperature. I'd like to hear pros and cons of each and any references stating one practice or the other.<< Opening that freezer-cold bottle before it warms up is ruinous. What I was taught to do in a research histochemistry lab (yeeks, this is a little less than 50 years ago) is to weigh out the needed quantity of the chemical (say, 10 mg of ATP) into empty gelatin capsules, and put the filled capsules in the freezer. When you do the procedure, take a single capsule out of the freezer. You could make up big batches of these capsules on a slow day. Bob Richmond Samurai Pathologist Knoxville TN From melissa at alliedsearchpartners.com Tue May 29 14:47:43 2018 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Tue, 29 May 2018 19:47:43 +0000 Subject: [Histonet] Histology Sales Representative Job Opening Message-ID: Hello All, I hope you had a great holiday weekend! I am currently sourcing candidates for a Histology Sales Representative Job Opening in Michigan. I am looking for ideally a candidate with histology and sales/client services experience willing to travel extensively throughout OH, IN, KY, WV, VA, PA and MI. I am looking for candidates locally or regionally located to the Kalamazoo, MI area. Please reach out to me if you are interested and I will follow up with more details. Thank you all. Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 Direct Line: 407.413.9117 F: 888.388.7572 From tmcampbe at fmh.org Wed May 30 07:15:22 2018 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Wed, 30 May 2018 12:15:22 +0000 Subject: [Histonet] Masson's Trichrome Troubleshooting Message-ID: Hello all, I am having issues with my trichrome stain and I am about to lose my mind! We just started doing it in house (although at my previous job I had done trichrome by hand for years so I am not a stranger to it. And I never had issues with it). When I brought it in house at my current lab, I ordered the same kit that I was familiar with. Its PolySci. I did the stain about 5 or 6 times and then all the sudden it quit working. There was red where it should be blue. And there was blue staining but it was all in the crypts. I tried tweaking the stain a few times and nothing worked so I got a new kit. The first 2 times I used the new kit, it worked perfect! But after that it is back to doing the same thing again! The collagen is not staining blue. It is staining red. Can anyone please tell me why this is happening? I never had this issue before! Thanks in advance!! See my protocol below. 1. Mordant in Bouin's solution, microwave 5 minutes, allow to stand 15 minutes. 2. Wash in running tap water to remove the picric acid, 5 minutes. 3. Weigert's Working Hematoxylin, 10 minutes. 4. Blue in running tap water for 5 minutes, rinse in distilled water. 5. Biebrich scarlet for 5 minutes. 6. Rinse in distilled water. 7. Phosphotungstic/phosphomolybdic acid for 10 minutes. 8. Transfer directly into Aniline blue for 5 minutes. 9. Rinse in distilled water. 10. 1% Acetic acid for 1 minute. 11. Quick rinse and air dry. 12. Coverslip Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 From aeck at dh.org Wed May 30 07:25:04 2018 From: aeck at dh.org (Eck, Allison) Date: Wed, 30 May 2018 12:25:04 +0000 Subject: [Histonet] frozen sections Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64AA70815E6@DH-MAIL01.dhorg.org> Question for those of you who do not have PA's...Who cuts the frozen sections, the techs or the pathologists? Thanks in advance Allison Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT),CEAS1 Lead Tech Histology Doylestown Hospital 595 W State St Doylestown, PA 18901 215-345-2264 aeck at dh.org From LNormington at uwhealth.org Wed May 30 08:15:40 2018 From: LNormington at uwhealth.org (Normington Lacy) Date: Wed, 30 May 2018 13:15:40 +0000 Subject: [Histonet] frozen sections In-Reply-To: <4ED8C96A8F20FC4F883A92E2A0A0D64AA70815E6@DH-MAIL01.dhorg.org> References: <4ED8C96A8F20FC4F883A92E2A0A0D64AA70815E6@DH-MAIL01.dhorg.org> Message-ID: My institution is an academic medical center; we have PA's, residents, specimen receiving technicians and histology technicians performing frozen sections. We perform a yearly competency of frozen sectioning for non MDs. Lacy Normington Lacy Normington, HTL(ASCP)CM Manager, Surgical Pathology Lab Services UW Health - UW Hospital 600 Highland Avenue Madison, WI 53792-2472 -----Original Message----- From: Eck, Allison via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, May 30, 2018 7:25 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] frozen sections WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Question for those of you who do not have PA's...Who cuts the frozen sections, the techs or the pathologists? Thanks in advance Allison Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT),CEAS1 Lead Tech Histology Doylestown Hospital 595 W State St Doylestown, PA 18901 215-345-2264 aeck at dh.org _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michael.gudo at morphisto.de Wed May 30 08:47:39 2018 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Wed, 30 May 2018 15:47:39 +0200 Subject: [Histonet] Masson's Trichrome Troubleshooting In-Reply-To: References: Message-ID: <5661355A-5F32-46EB-9F53-79FC9E49477D@morphisto.de> Dear Tasha, well, I think you should modify your protocol: (1) don?t use microwave (2) wash in ethanol after Bouin and not in water (70 - 80 %) (3) tap water as long as Weigert working solution (4) Bieberich Scarlet is not the correct stain for Masson, you should use Acid fuchsine + Ponceau 2R + Azophloxine (5) use a higher concentration of phosphortungstic acid, or / and least leave the sections much longer, up to 30 minutes in this step (6) do not ?air dry?, but remove water with ethanol and ethanol with xylene, then you should get very nice results. Well, and finally, if you allow I would like to offer you the Masson Kit / Masson Goldner Kit our company offers for quite brilliant stainings: https://webshop.morphisto.de/catalogsearch/result/?q=Kit+MASSON+trichrom Please do not hesitate to contact us, if you have any further questions. With best regards Michael > Am 30.05.2018 um 14:15 schrieb Campbell, Tasha M. via Histonet : > > Hello all, > > I am having issues with my trichrome stain and I am about to lose my mind! We just started doing it in house (although at my previous job I had done trichrome by hand for years so I am not a stranger to it. And I never had issues with it). When I brought it in house at my current lab, I ordered the same kit that I was familiar with. Its PolySci. I did the stain about 5 or 6 times and then all the sudden it quit working. There was red where it should be blue. And there was blue staining but it was all in the crypts. I tried tweaking the stain a few times and nothing worked so I got a new kit. The first 2 times I used the new kit, it worked perfect! But after that it is back to doing the same thing again! The collagen is not staining blue. It is staining red. Can anyone please tell me why this is happening? I never had this issue before! Thanks in advance!! See my protocol below. > > 1. Mordant in Bouin's solution, microwave 5 minutes, allow to stand 15 minutes. > 2. Wash in running tap water to remove the picric acid, 5 minutes. > 3. Weigert's Working Hematoxylin, 10 minutes. > 4. Blue in running tap water for 5 minutes, rinse in distilled water. > 5. Biebrich scarlet for 5 minutes. > 6. Rinse in distilled water. > 7. Phosphotungstic/phosphomolybdic acid for 10 minutes. > 8. Transfer directly into Aniline blue for 5 minutes. > 9. Rinse in distilled water. > 10. 1% Acetic acid for 1 minute. > 11. Quick rinse and air dry. > 12. Coverslip > > > > > Tasha Campbell, B.S.,HTL(ASCP) > Frederick Gastroenterology Associates > 310 W. 9th St. > Frederick, MD 21701 > 301-695-6800 ext. 144 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From relia1 at earthlink.net Wed May 30 11:34:51 2018 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 30 May 2018 12:34:51 -0400 Subject: [Histonet] Mohs Histotech Needed in Philadelphia A RELIA EXCLUSIVE CAN YOU HELP??? Message-ID: <00bc01d3f834$2238c510$66aa4f30$@earthlink.net> Hi Histonetters, How are you? I hope you are having a great week! I was hoping you might be able to help me. I am presently working with a client near Philadelphia that is in need of a Mohs histotech to run their own lab. This is a full time permanent DAY shift position. ASCP certification is preferred and 2 years of experience and CLIA qualifications to gross are required. Histonetters, the position involves performing routine histology, grossing and Mohs. The help I need from you is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them? (remember if I place someone you refer to me you will earn a 250.00 referral fee.) This is a RELIA Exclusive and my client offers a competitive compensation package and a great place to work!!!! If you are interested in this position please contact me ASAP toll free at 866-607-3542, cell/text 407-353-5070 or via email at relia1 at earthlink.net If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won't release it to anyone without your permission. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From melissa at alliedsearchpartners.com Wed May 30 12:22:13 2018 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Wed, 30 May 2018 17:22:13 +0000 Subject: [Histonet] CORRECTION: Histology Sales Position out of Columbus, OH Message-ID: Hello All, I sincerely apologize. I wanted to edit the fact that my last job announcement advertised a Histology Sales position in Kalamazoo, MI. I am actually sourcing for candidates in the Columbus, OH or nearby areas. Please let me know if you are interested and in the Columbus, OH area or immediate surrounding areas. Thank you so much! Have a great rest of your week! Melissa Owens, CHP (ASA) President, Laboratory Staffing T: 888.388.7571 ext. 102 Direct Line: 407.413.9117 F: 888.388.7572 From POWELL_SA at mercer.edu Wed May 30 14:22:35 2018 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Wed, 30 May 2018 19:22:35 +0000 Subject: [Histonet] Slide disposal Message-ID: <8b25f368353e4a5bbc3cb11d12e40b80@spiderman.MercerU.local> Hi Histonetters, mostly in the southeastern states, i.e. Georgia or South Carolina. I need to dispose of some years of slides that has patient identifying info on them, are there any companies that grind slides in the state of Georgia? I know there are companies that sell machines for this but I don't have the budget nor the volume for buying one. Thanks for your assistance in advance. Shirley A Powell, HTL(ASCP) Technical Director Histology Curricular Lab Mercer University Pathology Department 1501 Mercer University Drive. Macon, GA 31207-0001 PH: 478-301-2374 FX: 478-301-5489 Email: Powell_sa at mercer.edu From katherine at ka-recruiting.com Wed May 30 14:46:12 2018 From: katherine at ka-recruiting.com (Katherine Marano) Date: Wed, 30 May 2018 15:46:12 -0400 Subject: [Histonet] Histotech position Message-ID: Hi Histonetters, I am working with a client looking to hire a permanent and full-time Histotech in the Salem, Oregon area. Great laboratory! There would be relocation offered up to $3k. Salary is based on experience. Exact shift not determined yet, but likely early morning start. Anyone interested? Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From pwnoyce at gmail.com Wed May 30 18:21:56 2018 From: pwnoyce at gmail.com (Peter Noyce) Date: Thu, 31 May 2018 09:21:56 +1000 Subject: [Histonet] RNA preservation in Farmers solution Message-ID: <000001d3f86d$024cd5f0$06e681d0$@gmail.com> How long can tissue be stored in Farmers Solution (ethanol + acetic acid) before the RNA degrades. PW Noyce PhD student From gu.lang at gmx.at Thu May 31 00:53:56 2018 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 31 May 2018 07:53:56 +0200 Subject: [Histonet] Masson's Trichrome Troubleshooting In-Reply-To: References: Message-ID: <001801d3f8a3$c47a8d80$4d6fa880$@gmx.at> Hi, Check the staining after the PTA/PMA step. The acid should destain all the tissue areas, where afterwards the anilinblue should bind. The acid replaces the Biebrich scarlett in this areas and enables and enhances the binding of anilinblue. If the differentiation in insufficient, it may be the quality of the acid to be the culprit. The pH should be about 2, if I remember correctly. Check the temperature of the bouin after the microwave. If you cook the tissue, it may also change the stainability. Microwave is not microwave, the instrument in your old lab may have different characteristics. A softer way is 60? for two hours in the oven. Gudrun -----Urspr?ngliche Nachricht----- Von: Campbell, Tasha M. via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 30. Mai 2018 14:15 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Masson's Trichrome Troubleshooting Hello all, I am having issues with my trichrome stain and I am about to lose my mind! We just started doing it in house (although at my previous job I had done trichrome by hand for years so I am not a stranger to it. And I never had issues with it). When I brought it in house at my current lab, I ordered the same kit that I was familiar with. Its PolySci. I did the stain about 5 or 6 times and then all the sudden it quit working. There was red where it should be blue. And there was blue staining but it was all in the crypts. I tried tweaking the stain a few times and nothing worked so I got a new kit. The first 2 times I used the new kit, it worked perfect! But after that it is back to doing the same thing again! The collagen is not staining blue. It is staining red. Can anyone please tell me why this is happening? I never had this issue before! Thanks in advance!! See my protocol below. 1. Mordant in Bouin's solution, microwave 5 minutes, allow to stand 15 minutes. 2. Wash in running tap water to remove the picric acid, 5 minutes. 3. Weigert's Working Hematoxylin, 10 minutes. 4. Blue in running tap water for 5 minutes, rinse in distilled water. 5. Biebrich scarlet for 5 minutes. 6. Rinse in distilled water. 7. Phosphotungstic/phosphomolybdic acid for 10 minutes. 8. Transfer directly into Aniline blue for 5 minutes. 9. Rinse in distilled water. 10. 1% Acetic acid for 1 minute. 11. Quick rinse and air dry. 12. Coverslip Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TNMayer at mdanderson.org Thu May 31 10:14:12 2018 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Thu, 31 May 2018 15:14:12 +0000 Subject: [Histonet] Masson's Trichrome Troubleshooting Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC883ABDC42D@D1PWPEXMBX06.mdanderson.edu> Tasha, Are you using the PolySci kit k037? Did they change the formulation of the Bouins? They do make one that has no picric acid, and doesn't last long. If so, add a some saturated picric acid to the Bouins to see if that helps. Are you using the Bouins again after microwaving? If so, that may be your problem. Don't re-use the Phosphotungstic/phosphomolybdic acid, more than 2x. It sets and diff's the red. After the Anile blue, is the tissue blue? If not, then it's the ppa/pma is the problem. Use the acetic acid more sparingly. That diff's the blue some and can take more of it out. I do it by sight. Carson's says 3-5 min, but that is way too long. We don't air dry, but that wouldn't take out the blue. Let me know if this helps. Toysha N. Mayer D.H.Sc., MBA, HT(ASCP) Instructor/Education Coordinator HTL Program MD Anderson School of Health Professions 713.563.3481 tnmayer at mdanderson.org ------------------------------ Message: 2 Date: Wed, 30 May 2018 12:15:22 +0000 From: "Campbell, Tasha M." To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Masson's Trichrome Troubleshooting Message-ID: Content-Type: text/plain; charset="us-ascii" Hello all, I am having issues with my trichrome stain and I am about to lose my mind! We just started doing it in house (although at my previous job I had done trichrome by hand for years so I am not a stranger to it. And I never had issues with it). When I brought it in house at my current lab, I ordered the same kit that I was familiar with. Its PolySci. I did the stain about 5 or 6 times and then all the sudden it quit working. There was red where it should be blue. And there was blue staining but it was all in the crypts. I tried tweaking the stain a few times and nothing worked so I got a new kit. The first 2 times I used the new kit, it worked perfect! But after that it is back to doing the same thing again! The collagen is not staining blue. It is staining red. Can anyone please tell me why this is happening? I never had this issue before! Thanks in advance!! See my protocol below. 1. Mordant in Bouin's solution, microwave 5 minutes, allow to stand 15 minutes. 2. Wash in running tap water to remove the picric acid, 5 minutes. 3. Weigert's Working Hematoxylin, 10 minutes. 4. Blue in running tap water for 5 minutes, rinse in distilled water. 5. Biebrich scarlet for 5 minutes. 6. Rinse in distilled water. 7. Phosphotungstic/phosphomolybdic acid for 10 minutes. 8. Transfer directly into Aniline blue for 5 minutes. 9. Rinse in distilled water. 10. 1% Acetic acid for 1 minute. 11. Quick rinse and air dry. 12. Coverslip Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 ------------------------------ Message: 5 Date: Wed, 30 May 2018 15:47:39 +0200 From: "Dr. Michael Gudo (Morphisto GmbH)" To: "Campbell, Tasha M." Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Masson's Trichrome Troubleshooting Message-ID: <5661355A-5F32-46EB-9F53-79FC9E49477D at morphisto.de> Content-Type: text/plain; charset=utf-8 Dear Tasha, well, I think you should modify your protocol: (1) don?t use microwave (2) wash in ethanol after Bouin and not in water (70 - 80 %) (3) tap water as long as Weigert working solution (4) Bieberich Scarlet is not the correct stain for Masson, you should use Acid fuchsine + Ponceau 2R + Azophloxine (5) use a higher concentration of phosphortungstic acid, or / and least leave the sections much longer, up to 30 minutes in this step (6) do not ?air dry?, but remove water with ethanol and ethanol with xylene, then you should get very nice results. Well, and finally, if you allow I would like to offer you the Masson Kit / Masson Goldner Kit our company offers for quite brilliant stainings: https://webshop.morphisto.de/catalogsearch/result/?q=Kit+MASSON+trichrom Please do not hesitate to contact us, if you have any further questions. With best regards Michael The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From CDavis at che-east.org Thu May 31 10:54:08 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Thu, 31 May 2018 15:54:08 +0000 Subject: [Histonet] who cut the frozen Message-ID: <0782324ccef049a581856340e856a8b2@che-east.org> Allison, Here and the other three labs I worked at full-time, if the frozen arrived during "scheduled hisology hours", the histology techs cut them as long as it wouldn't accrue OT, after the techs left if the PA was still there, the PA would cut them , if the histology techs and PA left, then the pathologist would cut them. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From criley at dpspa.com Thu May 31 12:02:36 2018 From: criley at dpspa.com (Charles Riley) Date: Thu, 31 May 2018 13:02:36 -0400 Subject: [Histonet] Her2-ish Message-ID: Does anyone run her2 ish on the Leica Bonds. If so who do you purchase your antibody from? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From Teresa.Lima at bmc.org Thu May 31 13:14:45 2018 From: Teresa.Lima at bmc.org (Lima, Teresa) Date: Thu, 31 May 2018 18:14:45 +0000 Subject: [Histonet] Ventana Optiview kit Message-ID: <6c13d06391c644409fb8c884c17f5e6d@bmc.org> Hello Histonetters, Is anyone having issues with increased background staining of routine antibodies like keratins, CD markers and neuroendocrine markers using Ventana's antibodies with the Optiview kit? We are down to 4 minute antigen retrieval and primary antibody incubation times and still getting nonspecific staining. Our medical director thinks their prediluted antibodies are too concentrated for the kit. There are just a handful of their antibodies that are approved for this kit so there are no protocols in the package inserts. When we ran their breast markers with Optiview the positivity rate was incomparable to the results we get with UltraView and since the breast markers are not FDA cleared for OptiView we did not switch them over. It made our pathologists very apprehensive that with OptiView our Her2 validation cases became very positive and did not correlate with our UltraView stained slides and our FISH results. I look forward to your insights. Thanks everyone! Terri This electronic transmission may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, please notify me immediately as use of this information is strictly prohibited. From Teresa.Lima at bmc.org Thu May 31 13:18:12 2018 From: Teresa.Lima at bmc.org (Lima, Teresa) Date: Thu, 31 May 2018 18:18:12 +0000 Subject: [Histonet] FW: Ventana Optiview kit In-Reply-To: <6c13d06391c644409fb8c884c17f5e6d@bmc.org> References: <6c13d06391c644409fb8c884c17f5e6d@bmc.org> Message-ID: Hello Histonetters, Is anyone having issues with increased background staining of routine antibodies like keratins, CD markers and neuroendocrine markers using Ventana's antibodies with the Optiview kit? We are down to 4 minute antigen retrieval and primary antibody incubation times and still getting nonspecific staining. Our medical director thinks their prediluted antibodies are too concentrated for the kit. There are just a handful of their antibodies that are approved for this kit so there are no protocols in the package inserts. When we ran their breast markers with Optiview the positivity rate was incomparable to the results we get with UltraView and since the breast markers are not FDA cleared for OptiView we did not switch them over. It made our pathologists very apprehensive that with OptiView our Her2 validation cases became very positive and did not correlate with our UltraView stained slides and our FISH results. I look forward to your insights. Thanks everyone! Terri This electronic transmission may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, please notify me immediately as use of this information is strictly prohibited. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kelly.Pairan at nationwidechildrens.org Thu May 31 15:54:58 2018 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Thu, 31 May 2018 20:54:58 +0000 Subject: [Histonet] GMS Positive Fungal Tissue Needed Message-ID: <4c752c19af9c42d9b037c43086dd8216@l1perdwmbx01.childrensroot.net> Good Afternoon, We are in need of some GMS positive fungus tissue for positive controls. Would anyone out there happen to have some blocks we could have? We would be willing to swap if we had something you needed. We would also cover the cost of shipping. Thanks, Kelly Kelly Pairan, HT (ASCP)CM, QIHC (ASCP) Specialist II-Anatomic Pathology Department of Pathology and Laboratory Medicine Email: kelly.pairan at nationwidechildrens.org ph: 614-722-5414 fx: 614-722-3033 From leonard at rrclinicallab.com Thu May 31 18:24:00 2018 From: leonard at rrclinicallab.com (leonard at rrclinicallab.com) Date: Thu, 31 May 2018 18:24:00 -0500 Subject: [Histonet] GMS Positive Fungal Tissue Needed In-Reply-To: <4c752c19af9c42d9b037c43086dd8216@l1perdwmbx01.childrensroot.net> References: <4c752c19af9c42d9b037c43086dd8216@l1perdwmbx01.childrensroot.net> Message-ID: <902241949ad0d7184431bbc57d3a4de9@rrclinicallab.com> Hi Kelly We have blocks that you could have. We are currently looking for gram negative, and AFB positive controls if you have any please let us know. Please let us know if you have any of those and we can work something out. Thanks, Leonard Ringo Macneal Hospital Anatomic Pathology 3231 s Euclid ave. 708. 783.7441 Leonard at rrclinicallab.com On 2018-05-31 03:54 PM, Pairan, Kelly via Histonet wrote: > Good Afternoon, > We are in need of some GMS positive fungus tissue for positive controls. Would anyone out there happen to have some blocks we could have? We would be willing to swap if we had something you needed. We would also cover the cost of shipping. > > Thanks, > Kelly > > Kelly Pairan, HT (ASCP)CM, QIHC (ASCP) > Specialist II-Anatomic Pathology > Department of Pathology and Laboratory Medicine > Email: kelly.pairan at nationwidechildrens.org > ph: 614-722-5414 > fx: 614-722-3033 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet