From Kelly.Pairan at nationwidechildrens.org Thu Mar 1 12:53:23 2018 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Thu, 1 Mar 2018 18:53:23 +0000 Subject: [Histonet] Positive Tissues Needed Message-ID: Good Afternoon Histoland, We are currently working up a few antibodies and need some additional positive cases. We are looking for positive parvovirus cases , BK virus and GIST cases. We would be happy with some precut slides or a block if you can spare one. We would be willing to do a trade if we have an excess of something your institution needs. If you could help us out, please contact me via email kelly.pairan at nationwidechildrens.org Thanks for your help, Kelly Kelly Pairan, HT (ASCP)CM, QIHC (ASCP) Specialist II-Anatomic Pathology Department of Pathology and Laboratory Medicine Email: kelly.pairan at nationwidechildrens.org ph: 614-722-5414 fx: 614-722-3033 From jamie at watson-home.com Sat Mar 3 19:00:36 2018 From: jamie at watson-home.com (Jamie Watson) Date: Sun, 4 Mar 2018 01:00:36 +0000 Subject: [Histonet] Full time Histology position Carlsbad Ca. Message-ID: Ionis Pharmaceuticals has a fulltime position open in Carlsbad, Ca. https://rew12.ultipro.com/ISI1000/JobBoard/ListJobs.aspx Ionis Pharmaceuticals, Inc., located in the North San Diego County city of Carlsbad, was founded in 1989 to create advanced, novel medicines to treat diseases with an unmet medical need. We are the pioneer and leader in the discovery and development of antisense drugs. By exploiting various cutting-edge RNA targeting technologies, we are capable of specifically and efficiently targeting any gene of interest, including ones which are not targetable by small molecules or antibodies. We currently have three approved drugs, and a robust pipeline of over 30 drugs in various stages of clinical trials. Ionis is a challenging, motivating and rewarding environment designed to foster innovation and scientific excellence. Our success is a direct result of our outstanding employees. We are interested in bringing together individuals with different skill sets into this creative environment to be part of a productive team. Join us and experience our unique culture while you develop and expand your career. RESEARCH ASSSOCIATE - HISTOLOGY RESPONSIBILITIES: * Prepare microscopic slides on human/animal tissue for research/diagnostic purposes, including processing, embedding, microtomy, and mounting to include both paraffin processed tissue and frozen sectioning * Performs complex histochemical and immunohistochemical stains, records data, and maintains instruments * Quality control and quality assurance procedures, performing routine and special staining * Slide organization, prepares and maintains all necessary reagents, including stains, alcohols, and paraffins according to proper specifications * Handling and disposing of hazardous materials * Processes specimen accessioning to include all manual and computerized data entry * Performs other duties as assigned REQUIREMENTS: * 1-3 years of relevant experience * Knowledge of operation of general laboratory and automated IHC equipment * Understanding of general research concepts * Interpersonal/human relations skills * Computer data entry * Proficiency in algebraic equations * Ability to approach problems/decisions analytically * Ability to maintain records * Ability to use microscope, cryostat, tissue processors, and stainers * Ability/willingness to adhere to the established departmental service standards * Ability to be proactive and successful in an intense work environment * Willingness to work with hazardous chemicals * Ability to work in a lab environment where frequent standing, pushing and lifting is required * Ability to carry through on assignments and meet deadlines according to time schedules * Ability to be flexible with workload and job duties to support team objectives * Ability to work independently * Strong interpersonal skills and ability to be successful in a team environment MINIMUM QUALIFICATIONS: * Education and experience equivalent to: Associate's degree * HT(ASCP) Histotechnician certification preferred From LRaff at uropartners.com Tue Mar 6 09:08:37 2018 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 6 Mar 2018 15:08:37 +0000 Subject: [Histonet] Pathology Blog Post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF118FB2AA@COLOEXCH01.uropartners.local> Hope all are doing well. More on Prostate Cancer-Do the numbers add up? Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From relia1 at earthlink.net Tue Mar 6 10:22:15 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 6 Mar 2018 11:22:15 -0500 Subject: [Histonet] March 10th is Histotechnology Professionals Day! Message-ID: <000001d3b567$4af22b00$e0d68100$@earthlink.net> Hi Histonetters, Happy Histotechnology Professionals Day! I want to thank you not only for the job that you do and the lives that you save but also for allowing me to work with you in your careers. In all my years of recruiting I have never worked with a more professional, appreciative, honest and truly enjoyable group of people. Kudos to you!! I am curious as to how you are planning on celebrating. I started last week by contacting each and every one of my 1000+ client/employers to let them know that March 10 is National Histotechnology Professionals Day. Hopefully, if they weren't already doing something to show their appreciation they are now!! Since HPD falls on a Saturday maybe it is a good time to reflect on your career, where you are, what you want, where you would like to be and how are you going to get there? If you aren?t considering a job change: ? Is it time to join/renew your NSH membership? ? Check out your state society? ? Join the Histonet? ? Take some CEUs to stay up to date and learn some new skills? ? Get that state license for the place you want to move to one day? ? Start studying for that QIHC? If you are or might be considering a job change now or in the near future: ? Does your resume need to be updated? ? Have you considered what you are looking for and where? ? Have you gotten the state license if it is required before you go? ? Have we spoken recently? Please feel free to contact me by phone toll free at 866-607-3542 or email: relia1 at earthlink.net if you want information or help with any of the items listed. My services are always free of charge to you. If you have any friends that might benefit from receiving this please feel free to pass it along. I Currently Have Amazing Leadership Opportunities In: ? Alabama ? California ? Florida I have exciting opportunities for Histology Techs in: ? Alabama ? California ? North Carolina ? Virginia, ? Maryland ? Missouri If you would like more info on any of these opportunities or help with a job search in another area please shoot me an email at relia1 at earthlink.net to set up a time to chat at YOUR convenience!! Don?t forget to shoot me back an email to share what you did for Histotechnology Professionals Day. I really want to know ? Happy Histo Day!! >Pam Thank You! Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.comPamBarkerRELIA www.linkedin.com/reliasolutions www.twitter.com/pamatrelia From VKurth at uwhealth.org Tue Mar 6 10:43:04 2018 From: VKurth at uwhealth.org (Kurth Virginia L.) Date: Tue, 6 Mar 2018 16:43:04 +0000 Subject: [Histonet] LFABP and PHH3 Abs Message-ID: Hi Kiranjit I saw a question you had out on histonet back in 2015 about LFABP antibody, did anyone respond? One of our doctors want to bring this antibody on. Thanks Ginny UW Hospital of WI vkurth at uwhealth.org From katherine at ka-recruiting.com Tue Mar 6 11:31:44 2018 From: katherine at ka-recruiting.com (Katherine Marano) Date: Tue, 6 Mar 2018 12:31:44 -0500 Subject: [Histonet] Happy Histotech Day! Message-ID: Hi Histonet members, Happy Histo Day!!! Are any of you looking for a new permanent, full-time position? I am working on some fantastic openings and would love to tell you more. My clients offer competitive compensation, complete benefits, and relocation assistance. See below, and if you are interested please send me a resume directly and a good time to talk! *Histology openings:* CT - Darien - Histotech (night shift) FL - Tampa - MOHS Tech, operating mobile van FL - Tampa - Histology Supervisor IN - East Chicago - Histotech (midnight & evening shift) ME - Scarborough - Histotech II (evening shift) MN - Minneapolis - Histotech (day shift, mon - Fri 8a-5p) NC - Charlotte - Histotech NY - New York - Histotech (Bachelors & NY License req'd) NY - White Plains - Histotech (1st & 2nd shifts open w/ rotating wknds) (IHC exp req'd) OH - Columbus - Histotech (1st shift) VA - Richmond - Histotechnologist (days) Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From LRaff at uropartners.com Tue Mar 6 12:12:15 2018 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 6 Mar 2018 18:12:15 +0000 Subject: [Histonet] Supervisory Position in Suburban Chicago Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF118FCE5C@COLOEXCH01.uropartners.local> UroPartners Lab, a private lab in Chicago's Western Suburbs is beginning a search for a Histology Supervisor due to the impending retirement of our superstar supervisor. Our staff of 4 histologists embeds, cut and stain approximately 150-200 biopsy blocks/day, as well as a performing a variety of automated IHC stains. The Supervisor position is full-time M-F. Candidates should have appropriate certification and leadership experience. Please email me at lraff at uropartners.com if interested in applying for this position. We are an equal opportunity employer. Thanks, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From histo at pathlab.us Tue Mar 6 12:15:23 2018 From: histo at pathlab.us (Histology) Date: Tue, 6 Mar 2018 18:15:23 +0000 Subject: [Histonet] Ventana Red Detection Kits Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD8206E0DC98@vdc01.domain.local> HELP!!! Ventana is out of all Red detection kits until the end of the month. We are a small lab that only uses the red detection. Is everyone switching to DAB or are there other options? If switching to DAB, are you revalidating every antibody? And how many cases per antibody? Thanks for all input. Mehndi Helgren From brannon at alliedsearchpartners.com Tue Mar 6 15:16:54 2018 From: brannon at alliedsearchpartners.com (Brannon Owens) Date: Tue, 6 Mar 2018 21:16:54 +0000 Subject: [Histonet] Part time Histotech job opening in Milwaukee, WI Message-ID: Good afternoon ? we have a client in the Milwaukee, WI area looking for a permanent, part time histotech. The ideal candidate should have 3+ years of experience including grossing and IHC. Hours are flexible, with about 15 per week, 6 of those on Saturdays and 4 weeks out of the year full time for vacation coverage. If interested please send an updated resume to brannon at alliedsearchpartners.com for review. Thank you! .--To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers Brannon Owens Recruitment Manager Allied Search Partners LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823 http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 Direct Line: 407.413.9421 F: 888.388.7572 From jvickroy at SpringfieldClinic.com Wed Mar 7 16:11:35 2018 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Wed, 7 Mar 2018 22:11:35 +0000 Subject: [Histonet] Slide labels or slide labeler Message-ID: <9B1A1501A800064397369BD8072E6BCA6DFE122B@E2k10DB2.springfieldclinic.com> We have used the older version Slidemate slide labelers from Thermofisher for the last three years. I think putting the patient information directly on the slide is preferred however the wear and tear on slide printers means higher maintenance costs and more down time. Thermofisher has a newer version of the Slidemate now that has been designed so that there is less wear and tear on the machines. Currently when one of the old labelers breaks down we have to send the instrument out and the cost is generally 1000 - 1500 for repair each time. It also seems that the older machines have to be sent in at least every other year. Our older machines have been sent in almost yearly. We are considering switching to a slide label making system by General Data. One of the downsides is that we have to apply the label to each slide so therefore we introduce another step where the slides could be mislabeled. The upside is that equipment costs are much less. A new thermal label printer runs around 700.00 where as the instrument that imprints the slide label on the slide runs between 12000 - 15000 per unit. We need three units. Thermo also offers a lease system. What I need to know is what has been the experience with the new Slidemate AS printers? What kind of maintenance issues have you encountered? Finally please let me know your latest experiences with both slide label printers versus slide labeling printers? Thanks for your input. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From victor_tobias at comcast.net Wed Mar 7 16:58:32 2018 From: victor_tobias at comcast.net (Victor) Date: Wed, 7 Mar 2018 14:58:32 -0800 Subject: [Histonet] Slide labels or slide labeler In-Reply-To: <9B1A1501A800064397369BD8072E6BCA6DFE122B@E2k10DB2.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA6DFE122B@E2k10DB2.springfieldclinic.com> Message-ID: James, I totally agree with your assessment of the Slidemate printers. Have not had any experience with the newer model. Applying slide labels shouldn?t be an issue when dealing with one block and slide(s) at a time. Some of this could depend upon your software available to you. Our system generated just the labels needed for the block you were cutting, on demand. You should be able to get a nice Zebra printer for under $700. Another of our labs used the new Sakura/Premera printer and really like it. Victor Sent from Mail for Windows 10 From: Vickroy, James via Histonet Sent: Wednesday, March 7, 2018 2:12 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Slide labels or slide labeler We have used the older version Slidemate slide labelers from Thermofisher for the last three years. I think putting the patient information directly on the slide is preferred however the wear and tear on slide printers means higher maintenance costs and more down time. Thermofisher has a newer version of the Slidemate now that has been designed so that there is less wear and tear on the machines. Currently when one of the old labelers breaks down we have to send the instrument out and the cost is generally 1000 - 1500 for repair each time. It also seems that the older machines have to be sent in at least every other year. Our older machines have been sent in almost yearly. We are considering switching to a slide label making system by General Data. One of the downsides is that we have to apply the label to each slide so therefore we introduce another step where the slides could be mislabeled. The upside is that equipment costs are much less. A new thermal label printer runs around 700.00 where as the instrument that imprints the slide label on the slide runs between 12000 - 15000 per unit. We need three units. Thermo also offers a lease system. What I need to know is what has been the experience with the new Slidemate AS printers? What kind of maintenance issues have you encountered? Finally please let me know your latest experiences with both slide label printers versus slide labeling printers? Thanks for your input. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Thu Mar 8 13:30:35 2018 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 8 Mar 2018 19:30:35 +0000 Subject: [Histonet] Prosthetic lens processing, cutting? Message-ID: Hi all, Does anyone have any experience with embedding an acrylic prosthetic lens for EM, or any other way ? We have a case where they wanted EM on a lens to identify a specific bacteria. We have never done it and it would take some work to figure it out. Also, I told them that unless there is some morphologic marker for the bacteria, EM would not help much identifying it. Any ideas? Any labs that handle this kind of thing? They are willing to send it out. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From lblazek at digestivespecialists.com Thu Mar 8 14:32:57 2018 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Thu, 8 Mar 2018 15:32:57 -0500 Subject: [Histonet] Slide labels or slide labeler In-Reply-To: References: <9B1A1501A800064397369BD8072E6BCA6DFE122B@E2k10DB2.springfieldclinic.com> Message-ID: <5A2BD13465E061429D6455C8D6B40E392A826E8032@IBMB7Exchange.digestivespecialists.com> I've used the Primera slide printer since it came out. I like it a lot. It needs it's maintenance kept up like anything else but other than cleaning the rollers there is very little else that needs cleaning. Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Victor via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 07, 2018 5:59 PM To: Vickroy, James; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Slide labels or slide labeler James, I totally agree with your assessment of the Slidemate printers. Have not had any experience with the newer model. Applying slide labels shouldn?t be an issue when dealing with one block and slide(s) at a time. Some of this could depend upon your software available to you. Our system generated just the labels needed for the block you were cutting, on demand. You should be able to get a nice Zebra printer for under $700. Another of our labs used the new Sakura/Premera printer and really like it. Victor Sent from Mail for Windows 10 From: Vickroy, James via Histonet Sent: Wednesday, March 7, 2018 2:12 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Slide labels or slide labeler We have used the older version Slidemate slide labelers from Thermofisher for the last three years. I think putting the patient information directly on the slide is preferred however the wear and tear on slide printers means higher maintenance costs and more down time. Thermofisher has a newer version of the Slidemate now that has been designed so that there is less wear and tear on the machines. Currently when one of the old labelers breaks down we have to send the instrument out and the cost is generally 1000 - 1500 for repair each time. It also seems that the older machines have to be sent in at least every other year. Our older machines have been sent in almost yearly. We are considering switching to a slide label making system by General Data. One of the downsides is that we have to apply the label to each slide so therefore we introduce another step where the slides could be mislabeled. The upside is that equipment costs are much less. A new thermal label printer runs around 700.00 where as the instrument that imprints the slide label on the slide runs between 12000 - 15000 per unit. We need three units. Thermo also offers a lease system. What I need to know is what has been the experience with the new Slidemate AS printers? What kind of maintenance issues have you encountered? Finally please let me know your latest experiences with both slide label printers versus slide labeling printers? Thanks for your input. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From badams at acadianagastro.com Fri Mar 9 12:48:14 2018 From: badams at acadianagastro.com (Brent Adams) Date: Fri, 9 Mar 2018 18:48:14 +0000 Subject: [Histonet] Histonet Digest, Vol 172, Issue 6 "Slide labels or slide Printer" In-Reply-To: References: Message-ID: I also have used Sakura Smart Write (Primera printers repackaged under the Sakura name) slide and cassette printers since they came out. I had some issues with the slide printers in the beginning but they have worked out all the bugs and greatly improved the machine. I do very little maintenance other than clean rollers on the slide printer and never had any issues with the cassette printer. I recommend them for Small Labs for sure. They have multi cassette holders for larger labs. Hope that helps Brent Adams ? BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Friday, March 9, 2018 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 172, Issue 6 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Prosthetic lens processing, cutting? (Morken, Timothy) 2. Re: Slide labels or slide labeler (Blazek, Linda) ---------------------------------------------------------------------- Message: 1 Date: Thu, 8 Mar 2018 19:30:35 +0000 From: "Morken, Timothy" To: Histonet Subject: [Histonet] Prosthetic lens processing, cutting? Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, Does anyone have any experience with embedding an acrylic prosthetic lens for EM, or any other way ? We have a case where they wanted EM on a lens to identify a specific bacteria. We have never done it and it would take some work to figure it out. Also, I told them that unless there is some morphologic marker for the bacteria, EM would not help much identifying it. Any ideas? Any labs that handle this kind of thing? They are willing to send it out. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center ------------------------------ Message: 2 Date: Thu, 8 Mar 2018 15:32:57 -0500 From: "Blazek, Linda" To: Victor , "Vickroy, James" Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Slide labels or slide labeler Message-ID: <5A2BD13465E061429D6455C8D6B40E392A826E8032 at IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="utf-8" I've used the Primera slide printer since it came out. I like it a lot. It needs it's maintenance kept up like anything else but other than cleaning the rollers there is very little else that needs cleaning. Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Victor via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 07, 2018 5:59 PM To: Vickroy, James; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Slide labels or slide labeler James, I totally agree with your assessment of the Slidemate printers. Have not had any experience with the newer model. Applying slide labels shouldn?t be an issue when dealing with one block and slide(s) at a time. Some of this could depend upon your software available to you. Our system generated just the labels needed for the block you were cutting, on demand. You should be able to get a nice Zebra printer for under $700. Another of our labs used the new Sakura/Premera printer and really like it. Victor Sent from Mail for Windows 10 From: Vickroy, James via Histonet Sent: Wednesday, March 7, 2018 2:12 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Slide labels or slide labeler We have used the older version Slidemate slide labelers from Thermofisher for the last three years. I think putting the patient information directly on the slide is preferred however the wear and tear on slide printers means higher maintenance costs and more down time. Thermofisher has a newer version of the Slidemate now that has been designed so that there is less wear and tear on the machines. Currently when one of the old labelers breaks down we have to send the instrument out and the cost is generally 1000 - 1500 for repair each time. It also seems that the older machines have to be sent in at least every other year. Our older machines have been sent in almost yearly. We are considering switching to a slide label making system by General Data. One of the downsides is that we have to apply the label to each slide so therefore we introduce another step where the slides could be mislabeled. The upside is that equipment costs are much less. A new thermal label printer runs around 700.00 where as the instrument that imprints the slide label on the slide runs between 12000 - 15000 per unit. We need three units. Thermo also offers a lease system. What I need to know is what has been the experience with the new Slidemate AS printers? What kind of maintenance issues have you encountered? Finally please let me know your latest experiences with both slide label printers versus slide labeling printers? Thanks for your input. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Histonet Info Page - lists.utsouthwestern.edu Mailing Lists lists.utsouthwestern.edu Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet ------------------------------ End of Histonet Digest, Vol 172, Issue 6 **************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. From tbraud at holyredeemer.com Fri Mar 9 13:30:27 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 9 Mar 2018 19:30:27 +0000 Subject: [Histonet] Identification of bacteria on an acrylic lens prosthesis Message-ID: <48E053DDF6CE074DB6A7414BA05403F8B2325A46@HRHEX02-HOS.holyredeemer.local> I'm certified in EM. My suggestion would be to use SEM as opposed to TEM. The scanning electron microscope (SEM) can also be useful to reveal morphological features of isolated organisms as well as for diagnosis. There are numerous articles citing this technique. I believe the UC Berkley has an SEM in their lab. I hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Prosthetic lens processing, cutting? (Morken, Timothy) Message: 1 Date: Thu, 8 Mar 2018 19:30:35 +0000 From: "Morken, Timothy" To: Histonet Subject: [Histonet] Prosthetic lens processing, cutting? Hi all, Does anyone have any experience with embedding an acrylic prosthetic lens for EM, or any other way ? We have a case where they wanted EM on a lens to identify a specific bacteria. We have never done it and it would take some work to figure it out. Also, I told them that unless there is some morphologic marker for the bacteria, EM would not help much identifying it. Any ideas? Any labs that handle this kind of thing? They are willing to send it out. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center *********************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From BZIMMERM at augusta.edu Fri Mar 9 13:43:06 2018 From: BZIMMERM at augusta.edu (Zimmerman, Billie) Date: Fri, 9 Mar 2018 19:43:06 +0000 Subject: [Histonet] SAVE THE DATE! HISTOPALOOZA 2018 APRIL 20-APRIL 22 Message-ID: TGIF everyone The Georgia Society for Histotechnology meeting will be at the Callaway Gardens Lodge, Pine Mountain, GA More details to follow, but for now save the date! Billie Zimmerman From blayjorge at gmail.com Sat Mar 10 09:43:45 2018 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Sat, 10 Mar 2018 10:43:45 -0500 Subject: [Histonet] What is the smallest diameter (easily available and cheap) syringe? Message-ID: Hi: I am preparing a device for blowing air into a tiny, subcylindrical structure (diameter << 1 mm) in some (non-human) organisms. Heat extruded Pasteur pipettes are good but too fragile for my taste. What is the smallest diameter (easily available and cheap) syringe? If you have any constructive suggestions, please email me directly. Cheers, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm From tbraud at holyredeemer.com Fri Mar 9 13:30:27 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 9 Mar 2018 19:30:27 +0000 Subject: [Histonet] Identification of bacteria on an acrylic lens prosthesis Message-ID: <48E053DDF6CE074DB6A7414BA05403F8B2325A46@HRHEX02-HOS.holyredeemer.local> I'm certified in EM. My suggestion would be to use SEM as opposed to TEM. The scanning electron microscope (SEM) can also be useful to reveal morphological features of isolated organisms as well as for diagnosis. There are numerous articles citing this technique. I believe the UC Berkley has an SEM in their lab. I hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Prosthetic lens processing, cutting? (Morken, Timothy) Message: 1 Date: Thu, 8 Mar 2018 19:30:35 +0000 From: "Morken, Timothy" To: Histonet Subject: [Histonet] Prosthetic lens processing, cutting? Hi all, Does anyone have any experience with embedding an acrylic prosthetic lens for EM, or any other way ? We have a case where they wanted EM on a lens to identify a specific bacteria. We have never done it and it would take some work to figure it out. Also, I told them that unless there is some morphologic marker for the bacteria, EM would not help much identifying it. Any ideas? Any labs that handle this kind of thing? They are willing to send it out. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center *********************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. From christopher.hayden at novartis.com Mon Mar 12 07:22:00 2018 From: christopher.hayden at novartis.com (Hayden, Christopher) Date: Mon, 12 Mar 2018 12:22:00 +0000 Subject: [Histonet] Histonet Digest, Vol 172, Issue 7 In-Reply-To: References: Message-ID: Good Day, Tim: I'll second what Terri said. My first concern with embedding a contact lens would be that the embedding media and/or transitional solvents would attack the lens and/and dislodge the bacteria. Unless you have a few lenses to do a method development pilot study with, I'd shy away from a full embedding. If you had to go the TEM route, maybe a plastic like LR White, as the dehydration doesn't have to be as aggressive. The SEM is probably the best tool for this sort of investigation; we've had luck with identifying bacterial strains on morphology alone with our SEM, or at least it got us into the ballpark enough for the investigation. Children's Colorado has an SEM in their facility. Cheers, -Chris Christopher Hayden Electron Microscopy / Digital Imaging LS - Specialty Laboratories T +1 862 778 7993 christopher.hayden at novartis.com Novartis Institutes for Biomedical Research Date: Fri, 9 Mar 2018 19:30:27 +0000 From: "Terri Braud" To: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] Identification of bacteria on an acrylic lens prosthesis Message-ID: <48E053DDF6CE074DB6A7414BA05403F8B2325A46 at HRHEX02-HOS.holyredeemer.local> Content-Type: text/plain; charset="us-ascii" I'm certified in EM. My suggestion would be to use SEM as opposed to TEM. The scanning electron microscope (SEM) can also be useful to reveal morphological features of isolated organisms as well as for diagnosis. There are numerous articles citing this technique. I believe the UC Berkley has an SEM in their lab. I hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Prosthetic lens processing, cutting? (Morken, Timothy) Message: 1 Date: Thu, 8 Mar 2018 19:30:35 +0000 From: "Morken, Timothy" To: Histonet Subject: [Histonet] Prosthetic lens processing, cutting? Hi all, Does anyone have any experience with embedding an acrylic prosthetic lens for EM, or any other way ? We have a case where they wanted EM on a lens to identify a specific bacteria. We have never done it and it would take some work to figure it out. Also, I told them that unless there is some morphologic marker for the bacteria, EM would not help much identifying it. Any ideas? Any labs that handle this kind of thing? They are willing to send it out. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center Christopher Hayden T +1 862 778 7993 christopher.hayden at novartis.com -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Saturday, March 10, 2018 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 172, Issue 7 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=MatAxjSKXHZSS171h5q_lpGJSc4DpJw9cmQV2a37oU8&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Histonet Digest, Vol 172, Issue 6 "Slide labels or slide Printer" (Brent Adams) 2. Re: Identification of bacteria on an acrylic lens prosthesis (Terri Braud) 3. SAVE THE DATE! HISTOPALOOZA 2018 APRIL 20-APRIL 22 (Zimmerman, Billie) 4. What is the smallest diameter (easily available and cheap) syringe? (Jorge A. Santiago-Blay) ---------------------------------------------------------------------- Message: 1 Date: Fri, 9 Mar 2018 18:48:14 +0000 From: Brent Adams To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Histonet Digest, Vol 172, Issue 6 "Slide labels or slide Printer" Message-ID: Content-Type: text/plain; charset="Windows-1252" I also have used Sakura Smart Write (Primera printers repackaged under the Sakura name) slide and cassette printers since they came out. I had some issues with the slide printers in the beginning but they have worked out all the bugs and greatly improved the machine. I do very little maintenance other than clean rollers on the slide printer and never had any issues with the cassette printer. I recommend them for Small Labs for sure. They have multi cassette holders for larger labs. Hope that helps Brent Adams ? BS, LPN, HT https://urldefense.proofpoint.com/v2/url?u=http-3A__www.acadianagastro.com&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=Dzzc938ChQJl3YEQb3wQdhPZLoBnOMy2id1HRrn_bLc&e= Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Friday, March 9, 2018 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 172, Issue 6 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=MatAxjSKXHZSS171h5q_lpGJSc4DpJw9cmQV2a37oU8&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Prosthetic lens processing, cutting? (Morken, Timothy) 2. Re: Slide labels or slide labeler (Blazek, Linda) ---------------------------------------------------------------------- Message: 1 Date: Thu, 8 Mar 2018 19:30:35 +0000 From: "Morken, Timothy" To: Histonet Subject: [Histonet] Prosthetic lens processing, cutting? Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, Does anyone have any experience with embedding an acrylic prosthetic lens for EM, or any other way ? We have a case where they wanted EM on a lens to identify a specific bacteria. We have never done it and it would take some work to figure it out. Also, I told them that unless there is some morphologic marker for the bacteria, EM would not help much identifying it. Any ideas? Any labs that handle this kind of thing? They are willing to send it out. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center ------------------------------ Message: 2 Date: Thu, 8 Mar 2018 15:32:57 -0500 From: "Blazek, Linda" To: Victor , "Vickroy, James" Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Slide labels or slide labeler Message-ID: <5A2BD13465E061429D6455C8D6B40E392A826E8032 at IBMB7Exchange.digestivespecialists.com> Content-Type: text/plain; charset="utf-8" I've used the Primera slide printer since it came out. I like it a lot. It needs it's maintenance kept up like anything else but other than cleaning the rollers there is very little else that needs cleaning. Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Victor via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 07, 2018 5:59 PM To: Vickroy, James; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Slide labels or slide labeler James, I totally agree with your assessment of the Slidemate printers. Have not had any experience with the newer model. Applying slide labels shouldn?t be an issue when dealing with one block and slide(s) at a time. Some of this could depend upon your software available to you. Our system generated just the labels needed for the block you were cutting, on demand. You should be able to get a nice Zebra printer for under $700. Another of our labs used the new Sakura/Premera printer and really like it. Victor Sent from Mail for Windows 10 From: Vickroy, James via Histonet Sent: Wednesday, March 7, 2018 2:12 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Slide labels or slide labeler We have used the older version Slidemate slide labelers from Thermofisher for the last three years. I think putting the patient information directly on the slide is preferred however the wear and tear on slide printers means higher maintenance costs and more down time. Thermofisher has a newer version of the Slidemate now that has been designed so that there is less wear and tear on the machines. Currently when one of the old labelers breaks down we have to send the instrument out and the cost is generally 1000 - 1500 for repair each time. It also seems that the older machines have to be sent in at least every other year. Our older machines have been sent in almost yearly. We are considering switching to a slide label making system by General Data. One of the downsides is that we have to apply the label to each slide so therefore we introduce another step where the slides could be mislabeled. The upside is that equipment costs are much less. A new thermal label printer runs around 700.00 where as the instrument that imprints the slide label on the slide runs between 12000 - 15000 per unit. We need three units. Thermo also offers a lease system. What I need to know is what has been the experience with the new Slidemate AS printers? What kind of maintenance issues have you encountered? Finally please let me know your latest experiences with both slide label printers versus slide labeling printers? Thanks for your input. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=MatAxjSKXHZSS171h5q_lpGJSc4DpJw9cmQV2a37oU8&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=MatAxjSKXHZSS171h5q_lpGJSc4DpJw9cmQV2a37oU8&e= Histonet Info Page - https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=1sE1SSoyO-runtuK_WoVchPnoSApq7C-coCsWs8y0gg&e= Mailing Lists https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=1sE1SSoyO-runtuK_WoVchPnoSApq7C-coCsWs8y0gg&e= Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=MatAxjSKXHZSS171h5q_lpGJSc4DpJw9cmQV2a37oU8&e= Histonet Info Page - https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=1sE1SSoyO-runtuK_WoVchPnoSApq7C-coCsWs8y0gg&e= Mailing Lists https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=1sE1SSoyO-runtuK_WoVchPnoSApq7C-coCsWs8y0gg&e= Histonet -- For the exchange of information pertaining to histotechnology and related fields About Histonet ------------------------------ End of Histonet Digest, Vol 172, Issue 6 **************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. ------------------------------ Message: 2 Date: Fri, 9 Mar 2018 19:30:27 +0000 From: "Terri Braud" To: "'histonet at lists.utsouthwestern.edu'" Subject: Re: [Histonet] Identification of bacteria on an acrylic lens prosthesis Message-ID: <48E053DDF6CE074DB6A7414BA05403F8B2325A46 at HRHEX02-HOS.holyredeemer.local> Content-Type: text/plain; charset="us-ascii" I'm certified in EM. My suggestion would be to use SEM as opposed to TEM. The scanning electron microscope (SEM) can also be useful to reveal morphological features of isolated organisms as well as for diagnosis. There are numerous articles citing this technique. I believe the UC Berkley has an SEM in their lab. I hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. Prosthetic lens processing, cutting? (Morken, Timothy) Message: 1 Date: Thu, 8 Mar 2018 19:30:35 +0000 From: "Morken, Timothy" To: Histonet Subject: [Histonet] Prosthetic lens processing, cutting? Hi all, Does anyone have any experience with embedding an acrylic prosthetic lens for EM, or any other way ? We have a case where they wanted EM on a lens to identify a specific bacteria. We have never done it and it would take some work to figure it out. Also, I told them that unless there is some morphologic marker for the bacteria, EM would not help much identifying it. Any ideas? Any labs that handle this kind of thing? They are willing to send it out. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center *********************** --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ------------------------------ Message: 3 Date: Fri, 9 Mar 2018 19:43:06 +0000 From: "Zimmerman, Billie" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] SAVE THE DATE! HISTOPALOOZA 2018 APRIL 20-APRIL 22 Message-ID: Content-Type: text/plain; charset="us-ascii" TGIF everyone The Georgia Society for Histotechnology meeting will be at the Callaway Gardens Lodge, Pine Mountain, GA More details to follow, but for now save the date! Billie Zimmerman ------------------------------ Message: 4 Date: Sat, 10 Mar 2018 10:43:45 -0500 From: "Jorge A. Santiago-Blay" To: histonet at lists.utsouthwestern.edu Subject: [Histonet] What is the smallest diameter (easily available and cheap) syringe? Message-ID: Content-Type: text/plain; charset="UTF-8" Hi: I am preparing a device for blowing air into a tiny, subcylindrical structure (diameter << 1 mm) in some (non-human) organisms. Heat extruded Pasteur pipettes are good but too fragile for my taste. What is the smallest diameter (easily available and cheap) syringe? If you have any constructive suggestions, please email me directly. Cheers, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* https://urldefense.proofpoint.com/v2/url?u=http-3A__blaypublishers.com_testimonials_&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=_3zzySWXoTSNwwhKP1OATmU-uVIe0lp9_j3cOdLnARQ&e= 2. Free examples of papers published in *LEB*: https://urldefense.proofpoint.com/v2/url?u=http-3A__blaypublishers.com_category_previous-2Dissues_&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=C2Dlouw0YUh511iEBtc1kkvBoVND1_35YhsDcpqK2Xw&e=. 3. *Guidelines for Authors* and page charges of *LEB*: https://urldefense.proofpoint.com/v2/url?u=http-3A__blaypublishers.com_archives_&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=uehOhIvCm7C4YyqH_tqCq4q7tR6HZudXwWthWI1cmjE&e= *.* 4. Want to subscribe to *LEB*? https://urldefense.proofpoint.com/v2/url?u=http-3A__blaypublishers.com_subscriptions_&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=tAUxfakrhWzEwyxfhO1OibaV9k5B55v1EHneJzSghY8&e= https://urldefense.proofpoint.com/v2/url?u=http-3A__blayjorge.wordpress.com_&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=2oRa8t3wEZnHmETD4JeMstw_Oy10qujP3i7Jz64RYwQ&e= https://urldefense.proofpoint.com/v2/url?u=http-3A__paleobiology.si.edu_staff_individuals_santiagoblay.cfm&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=9Y3WUcIUlKAiofJQuytYrCrlaG5-MbCVFDos6UhK-ww&e= ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=ZbgFmJjg4pdtrnL2HUJUDw&r=oHNFb0vNgGhlfuzljZEWz1In4NMRNTEnF8z_Nz6USno&m=VFtPXj_ahjTodptDNNpwf48ccKM7L6xQMCWbtd7jFXU&s=MatAxjSKXHZSS171h5q_lpGJSc4DpJw9cmQV2a37oU8&e= ------------------------------ End of Histonet Digest, Vol 172, Issue 7 **************************************** From despinosa at stamhealth.org Mon Mar 12 09:35:55 2018 From: despinosa at stamhealth.org (despinosa at stamhealth.org) Date: Mon, 12 Mar 2018 10:35:55 -0400 Subject: [Histonet] Medical Technologists Opportunities at Stamford Health Message-ID: <004701d3ba0f$6e392170$4aab6450$@stamhealth.org> Medical Technologists Opportunities at Stamford Health Stamford, CT Make an Impact. And enrich your future. Stamford Hospital is seeking experienced Medical Technologists like you, who are ready for a tremendous opportunity to join a team where your ideas and input will be encouraged and the opportunity to learn and grow begins day one. As an organization of caregivers, we share a passionate pursuit of putting the patient first. Only by working together can we truly do our best to help our patients heal physically, mentally, emotionally, socially, and spiritually. Are you ready to put your lab skills to the test? Stamford Hospital and you, together we represent excellence in patient-centered care. Currently, we are seeking Generalist-Medical Technologists to cover evenings, as well as a Lead Medical Technologist for evening/night coverage. We also have day openings for Microbiology, Blood Bank and Core Hematology/Chemistry. You'll exercise your skills in our state-of-the-art CAP-accredited Laboratory department. All positions require rotating weekend and holiday coverage. As a Medical Technologist on our team, your responsibilities will include: * Independently performing and reporting general and/or specialized laboratory test * Providing technical services and laboratory support information to staff and providers As a qualified Medical Technologist, you must have: * Bachelor's degree in Medical Technology and additional specialty lab training * A minimum of 2 years of related medical technologist experience in the position of interest * ASCP BOC registry or registry eligible; or equivalent registry (AMT, NCA) * MLT(ASCP), MLS(ASCP), MT(AMT), CLS(NCA) * Additional ASCP BOC specialty registry or equivalent registry (AMT, NCA) * SBB(ASCP), BB(ASCP), SM(ASAP), M(ASCP) preferred * Previous lead or supervisory experience preferred (for the Lead Medical Technologist position) Stamford Hospital understands what it takes to attract talent to improve our workforce and support our mission, so we're offering you: * Up to $5,000 sign-on bonus based on experience; prorated for part-time * Competitive salaries, shift and weekend differentials * Low cost medical, dental, and vision insurance available day one * Generous PTO * Career Ladder for career advancement * And much more! Are you ready to take your career to the next level and be inspired and supported to make the most of your skills and ideas? Contact us today! You may apply online at: StamfordHealth.org/Careers or email your resume to Diana Espinosa, HR Partner, at: despinosa at stamhealth.org EOE M/F/D/V Stamford Health Designated: ANCC Magnet RecognitionR Accredited: The Joint Commission Member: New York-Presbyterian Healthcare System Designated: Planetree Person-Centered Care From john.frazier at roche.com Mon Mar 12 09:52:50 2018 From: john.frazier at roche.com (Frazier, John) Date: Mon, 12 Mar 2018 07:52:50 -0700 Subject: [Histonet] Slide labels or slide labeler Message-ID: As a AP workflow consultant, I look at the pro?s and con?s of each system and come up with a plan or product that best meets the needs of the individual lab. With that being said here are the variable I consider: 1. Size or foot print vs. work space 2. Cost per unit (also keep in mind additional units for growth and replacement units due to catastrophic failure) 3. Cost of consumables. Tape, labels 4. Do I have to buy the printer company?s slides 5. You should have one printer per microtomy work station to ensure single piece flow. Do not buy one printer from all slide printing which ensures batch slide printing. 6. Maintenance requirements, (Daily, weekly, etc.) Time to perform the maintenance. 7. Reliability, dependability, reputation and speed of the printer company or seller for maintenance and, solution resolutions 8. The print on the slide or label must be highly xylene and heat resistant for slide data integrity. If you cure your slides prior to storage, the label sometimes can turn black from the heat. 9. Is the printer going to be connected to a system that reads the Bar Code from the LIS in order to create the label or the individual labeled slide one at a time. 10. Speed of slide or label printing. 11. Frequency of loading the slides vs. labels 11. Will the label interfere with coverslipping And that?s all I got to say about that. Sent from my iPad On Mar 8, 2018, at 3:32 PM, Blazek, Linda wrote: I've used the Primera slide printer since it came out. I like it a lot. It needs it's maintenance kept up like anything else but other than cleaning the rollers there is very little else that needs cleaning. Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Victor via Histonet [mailto:histonet at lists.utsouthwestern.edu ] Sent: Wednesday, March 07, 2018 5:59 PM To: Vickroy, James; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Slide labels or slide labeler James, I totally agree with your assessment of the Slidemate printers. Have not had any experience with the newer model. Applying slide labels shouldn?t be an issue when dealing with one block and slide(s) at a time. Some of this could depend upon your software available to you. Our system generated just the labels needed for the block you were cutting, on demand. You should be able to get a nice Zebra printer for under $700. Another of our labs used the new Sakura/Premera printer and really like it. Victor Sent from Mail for Windows 10 From: Vickroy, James via Histonet Sent: Wednesday, March 7, 2018 2:12 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Slide labels or slide labeler We have used the older version Slidemate slide labelers from Thermofisher for the last three years. I think putting the patient information directly on the slide is preferred however the wear and tear on slide printers means higher maintenance costs and more down time. Thermofisher has a newer version of the Slidemate now that has been designed so that there is less wear and tear on the machines. Currently when one of the old labelers breaks down we have to send the instrument out and the cost is generally 1000 - 1500 for repair each time. It also seems that the older machines have to be sent in at least every other year. Our older machines have been sent in almost yearly. We are considering switching to a slide label making system by General Data. One of the downsides is that we have to apply the label to each slide so therefore we introduce another step where the slides could be mislabeled. The upside is that equipment costs are much less. A new thermal label printer runs around 700.00 where as the instrument that imprints the slide label on the slide runs between 12000 - 15000 per unit. We need three units. Thermo also offers a lease system. What I need to know is what has been the experience with the new Slidemate AS printers? What kind of maintenance issues have you encountered? Finally please let me know your latest experiences with both slide label printers versus slide labeling printers? Thanks for your input. Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Mon Mar 12 12:16:42 2018 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 12 Mar 2018 13:16:42 -0400 Subject: [Histonet] #hpd - Check it out!! Message-ID: <000001d3ba25$e4033c90$ac09b5b0$@earthlink.net> Hello Histonetters, I hope your week is off to a great start!! I know mine is!! I want to remind you once more about the hashtag #hpd Search the hashtag on Facebook, Instagram and Twitter to see all the fun your fellow histotechs had on Histotechnology Professionals Day!! I would also like to tell you about the positions I am currently working on. Here are the spotlight jobs I want to share with you! RELIA Spotlight Histology Job!!! Histology Tech ? Austin, TX Histotechnician ? Salem, VA Histology Tech ?Kansas City, MO Here are the rest of my sizzlin? hot opportunities!!! Histology Supervisor ? Tampa, FL Histology Supervisor ? Birmingham, AL Histology Supervisor ? Modesto, CA Grossing Histotech ? San Diego, CA Histology Tech ? Baltimore, MD Histotech ? Charlotte, NC Histotech ? Modesto, CA And new opportunities are coming in nationwide DAILY! If you are on the hunt and your desired location is not listed shoot me an email and tell me where you want to go so I can investigate for you. REMEMBER ? ALL INQUIRIES ARE KEPT CONFIDENTIAL! Unlike OTHER recruiting firms; Here at RELIA I will only show a client YOUR resume with YOUR permission. I respect YOU and YOUR career!! All of my clients offer excellent compensation, benefits and in most cases relocation assistance. These are full time permanent positions with stable secure labs. My clients are eager to interview and hire for these positions! If any of these opportunities are the right one for you RELIA can make it happen!!! If you or anyone you know is interested in any of these opportunities or are looking for a position in another area and want some help - Contact me!!!! I can be reached ASAP on my cell/text at 407-353-5070, via email at relia1 at earthlink.net or toll free at the office at 866-607-3542. If you refer someone and I place them you will earn a referral fee!! Don?t forget to checkout #hpd Have a great week!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From BZIMMERM at augusta.edu Mon Mar 12 14:51:19 2018 From: BZIMMERM at augusta.edu (Zimmerman, Billie) Date: Mon, 12 Mar 2018 19:51:19 +0000 Subject: [Histonet] Georgia Society for Histotechnology April 20 - 22, 2018 Message-ID: The annual Georgia Histopalooza will be held at Callaway Gardens. It's located in Pine Mountain, Georgia. Membership is free for the Georgia Society. The all-inclusive registration fee is $135. This includes the vendor registration, Award Luncheon and CEUs. The student registration fee is only $65. "Google" the event for the program information and registration form. More info to follow! From aeck at dh.org Wed Mar 14 07:22:23 2018 From: aeck at dh.org (Eck, Allison) Date: Wed, 14 Mar 2018 12:22:23 +0000 Subject: [Histonet] synoptic reporting Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64AA6FBFAB6@DH-MAIL01.dhorg.org> Good morning, I am interested in finding out the ways that other labs stay up to date and compliant with the synoptic reporting requirements. Our LIS software makes it quite difficult for us to integrate these as templates. Do most labs have this built into their LIS, is there external software or programs that you use, do your pathologists just take responsibility for getting this information into the report? Any advice is most appreciated Thanks Allison From Karen.Heckford at DignityHealth.org Wed Mar 14 07:49:55 2018 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Wed, 14 Mar 2018 12:49:55 +0000 Subject: [Histonet] Slide printer and cassette label Message-ID: <2580bbf894c24595921a1a894c7c5ccf@PHX-EXCH-013.chw.edu> Good Morning, I am looking for suggestions for a slide printer and cassette labeler for a smaller lab. Any suggestions. I need it with the two patient identifier. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you From lblazek at digestivespecialists.com Wed Mar 14 08:10:33 2018 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Wed, 14 Mar 2018 09:10:33 -0400 Subject: [Histonet] Slide printer and cassette label In-Reply-To: <2580bbf894c24595921a1a894c7c5ccf@PHX-EXCH-013.chw.edu> References: <2580bbf894c24595921a1a894c7c5ccf@PHX-EXCH-013.chw.edu> Message-ID: <5A2BD13465E061429D6455C8D6B40E392A82A28606@IBMB7Exchange.digestivespecialists.com> Karen, We print an average of 150 blocks a day and 250+ slides a day. We have 2 cassette printers and 1 slide printer. We have had them for several years now. They are Primera printers purchased from Creative Waste Solutions. https://cwsincorp.com/ We have had excellent results with them both in functionality and service. Also they can connect to your LIS if you want very easily without needing an expensive interface. Linda Linda Blazek HT (ASCP) Pathology Lab Manager Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 14, 2018 8:50 AM To: Histonet (E-mail) Subject: [Histonet] Slide printer and cassette label Good Morning, I am looking for suggestions for a slide printer and cassette labeler for a smaller lab. Any suggestions. I need it with the two patient identifier. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rob at foliobio.com Wed Mar 14 08:44:10 2018 From: rob at foliobio.com (Rob Day) Date: Wed, 14 Mar 2018 09:44:10 -0400 Subject: [Histonet] synoptic reporting In-Reply-To: <4ED8C96A8F20FC4F883A92E2A0A0D64AA6FBFAB6@DH-MAIL01.dhorg.org> References: <4ED8C96A8F20FC4F883A92E2A0A0D64AA6FBFAB6@DH-MAIL01.dhorg.org> Message-ID: I have no idea if it will work for you, but you might want to take a look at CERF ELN. It is flexible enough to record almost any sort of report document in a 21CFR11 compliant way. > On Mar 14, 2018, at 8:22 AM, Eck, Allison via Histonet > wrote: > > Good morning, > I am interested in finding out the ways that other labs stay up to date and compliant with the synoptic reporting requirements. Our LIS software makes it quite difficult for us to integrate these as templates. Do most labs have this built into their LIS, is there external software or programs that you use, do your pathologists just take responsibility for getting this information into the report? > > Any advice is most appreciated > Thanks > Allison > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Douglas.Porter at sparrow.org Wed Mar 14 11:09:29 2018 From: Douglas.Porter at sparrow.org (Porter, Douglas) Date: Wed, 14 Mar 2018 16:09:29 +0000 Subject: [Histonet] synoptic reporting In-Reply-To: <4ED8C96A8F20FC4F883A92E2A0A0D64AA6FBFAB6@DH-MAIL01.dhorg.org> References: <4ED8C96A8F20FC4F883A92E2A0A0D64AA6FBFAB6@DH-MAIL01.dhorg.org> Message-ID: <1ea072552ef64a0587d6f3f41a14f496@EXCHMBPVAS02.shs.org> Allison, The CAP has a program called Electronic Cancer Checklists (eCC) that you can purchase that will interface with your LIS (if compatible) and install / update the checklists as they are created by the CAP. We are in the final decision stage of installing it ourselves. If you are interested, let me know and I can forward you some documents I have that will explain the program. Or, you can contact the CAP and get things started with them. Hope this helps, Douglas A. Porter, HT (ASCP) Pathologist Assistant Anatomic Pathology IT Coordinator Sparrow Center for Laboratory Medicine Department of Pathology 3392 Patient Care Drive Lansing, MI 48911 517-371-9481 (phone) 517-371-9540 (fax) douglas.porter at sparrow.org -----Original Message----- From: Eck, Allison via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 14, 2018 8:22 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [EXTERNAL] [Histonet] synoptic reporting Warning: This email originated from outside of Sparrow. Do not click links or open attachments unless you recognize the sender and are expecting the message. ---------------------------------------------------------------------- Good morning, I am interested in finding out the ways that other labs stay up to date and compliant with the synoptic reporting requirements. Our LIS software makes it quite difficult for us to integrate these as templates. Do most labs have this built into their LIS, is there external software or programs that you use, do your pathologists just take responsibility for getting this information into the report? Any advice is most appreciated Thanks Allison _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=qgJBLQvENW4Kb9JcrSOXvjOKPeZu-lgWMRRjbRk-vYI&r=FB_j03RJqXZhrS71GzU-qcyfg0DRiu5KOssLLNE66qE&m=VlHq4PrUjilClHEsZ6F2QBmdtkvfFJ0c9eC9mSdSgyI&s=9TsRcbyZliWgxhmD5S_3yFXOUUubOAkO7M9A1XJoy6I&e= ________________________________ CONFIDENTIALITY NOTICE: This email communication may contain private, confidential, or legally privileged information intended for the sole use of the designated and/or duly authorized recipient(s). If you are not the intended recipient or have received this email in error, please notify the sender immediately by email and permanently delete all copies of this email including all attachments without reading them. If you are the intended recipient, secure the contents in a manner that conforms to all applicable state and/or federal requirements related to privacy and confidentiality of such information. ________________________________ From jkempf80 at uga.edu Wed Mar 14 12:05:09 2018 From: jkempf80 at uga.edu (Jennifer Kempf) Date: Wed, 14 Mar 2018 17:05:09 +0000 Subject: [Histonet] Slide printer and cassette label In-Reply-To: <5A2BD13465E061429D6455C8D6B40E392A82A28606@IBMB7Exchange.digestivespecialists.com> References: <2580bbf894c24595921a1a894c7c5ccf@PHX-EXCH-013.chw.edu> <5A2BD13465E061429D6455C8D6B40E392A82A28606@IBMB7Exchange.digestivespecialists.com> Message-ID: The Primera units have customizable templates so you can set them up to have as many identifiers as you need. We have had one of each for a little over a year now, and like both of them. We purchased ours from Cancer Diagnostics, though. Thanks, Jennifer M Kempf Histology Laboratory Manager Department of Pathology College of Veterinary Medicine University of Georgia 501 D.W. Brooks Drive Athens, Georgia 30602 Ph: 706-542-2218 Fax: 706-542-5828 jkempf80 at uga.edu -----Original Message----- From: Blazek, Linda [mailto:lblazek at digestivespecialists.com] Sent: Wednesday, March 14, 2018 9:11 AM To: Heckford, Karen - SMMC-SF ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Slide printer and cassette label Karen, We print an average of 150 blocks a day and 250+ slides a day. We have 2 cassette printers and 1 slide printer. We have had them for several years now. They are Primera printers purchased from Creative Waste Solutions. https://cwsincorp.com/ We have had excellent results with them both in functionality and service. Also they can connect to your LIS if you want very easily without needing an expensive interface. Linda Linda Blazek HT (ASCP) Pathology Lab Manager Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 14, 2018 8:50 AM To: Histonet (E-mail) Subject: [Histonet] Slide printer and cassette label Good Morning, I am looking for suggestions for a slide printer and cassette labeler for a smaller lab. Any suggestions. I need it with the two patient identifier. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Wed Mar 14 13:26:07 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 14 Mar 2018 18:26:07 +0000 Subject: [Histonet] Synoptic Reports Message-ID: <48E053DDF6CE074DB6A7414BA05403F8C1B88225@HRHEX02-HOS.holyredeemer.local> Hi Allison - We couldn't afford the program offered by our LIS vendor, but since they are published annually, we have a fabulous transcriptionist that updates and reformats all of ours every year. She saves them as a "quick text" in the LIS. They are accessible to all who are dictating/transcribing reports. It's not very sophisticated, but it is cheap, reproducible, easily implemented, and most of all, compliant. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. synoptic reporting (Eck, Allison) Good morning, I am interested in finding out the ways that other labs stay up to date and compliant with the synoptic reporting requirements. Our LIS software makes it quite difficult for us to integrate these as templates. Do most labs have this built into their LIS, is there external software or programs that you use, do your pathologists just take responsibility for getting this information into the report? Any advice is most appreciated Thanks Allison From jennc976 at gmail.com Wed Mar 14 14:45:33 2018 From: jennc976 at gmail.com (Jennifer Clark) Date: Wed, 14 Mar 2018 15:45:33 -0400 Subject: [Histonet] staffing and qualifications Message-ID: I am looking for some articles or something substantial that can help me approach the doctor I work for. The office manager (private lab and the office manager has no idea about working in the lab area) is trying to not replace my part time helper. There used to be 2 full time HTs until about 4 years ago. For the longest time it was just myself until I finally convinced the office manager to hire someone for at least PT. That PT helper is leaving and they tried to put a medical assistant up here with me 2 days a week who has no science background, only college is business. I need something to show that there needs to be someone with science background in college to embed, cut, stain, etc. I also need some information on how much is too much for one HT. Currently there are 2 doctors (only one read slides) and 3 Physician Assistants. About 6,500 cases for H&E (hand stained) last year, we do 5 IHC stains (automated), and 6 different special stains. Thank you, Jennifer From jmacdonald at mtsac.edu Thu Mar 15 01:18:39 2018 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Thu, 15 Mar 2018 06:18:39 +0000 Subject: [Histonet] CSH Annual Symposium Message-ID: Please join us for the California Society for Histotechnology Annual Symposium in Anaheim. Workshops and registration information can be found at: http://californiahistology.org/events.html From rjr6 at psu.edu Thu Mar 15 08:27:24 2018 From: rjr6 at psu.edu (Roberta Horner) Date: Thu, 15 Mar 2018 13:27:24 +0000 Subject: [Histonet] service contracts Message-ID: <219f8772ef7e4870beaafca898392167@PSU.EDU> The lab I work in decided to change service contractors on my equipment without consulting me. Does anyone know or have heard anything about REMI website is http://www.theremigroup.com/ Roberta Horner Animal Diagnostic Lab Pennsylvania State University From lmarie08 at uga.edu Thu Mar 15 10:45:27 2018 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Thu, 15 Mar 2018 15:45:27 +0000 Subject: [Histonet] Von Kossa falls off Message-ID: Hi All, My bone tissue falls right off at the first rinse- after the silver step. Ive tried charged and uncharged slides and been VERY gentle. Any tips? Thanks! From mgflem at gmail.com Fri Mar 16 08:00:00 2018 From: mgflem at gmail.com (Matthew Fleming) Date: Fri, 16 Mar 2018 08:00:00 -0500 Subject: [Histonet] Leica ST5050 immunostainer Message-ID: Hi, I have a small dermatopathology lab and would like to introduce limited immunohistochemistry. Initially I would probably just do MITF stains with red chromogen on a few cases per week. We could do this by hand but I've noticed a number of ST5050 immunostainers for sale at very low prices. I know they're quite old but we have a number of machines old or older that continue to serve us well. But so far I haven't been able to find a manual and am concerned about finding consumables. Leica apparently stopped supporting this model some time ago. Is anyone out there still using it? Anyone have a manual? Anyone like to comment as to whether it might be usable for a limited IH operation? Thank you, Matthew Fleming, MD Fleming Dermatopathology Milwaukee, WI From plucas at biopath.org Fri Mar 16 08:54:30 2018 From: plucas at biopath.org (Paula) Date: Fri, 16 Mar 2018 06:54:30 -0700 Subject: [Histonet] Antibody Validation CLIA Message-ID: <001801d3bd2e$4f4b0c20$ede12460$@biopath.org> Hello, We've been discussing about the quantity of slides to run as a validation for IHC antibodies. We are governed by CLIA, and we would like to know if there is a set number of slides to run for a particular antibody we would like to bring in-house for Validation. I think CAP requires 20 slides..? And so we are asking if there is a requirement with CLIA to run a certain number of slides, or is it up to us (the laboratory director) to decide how many slides to run for Validation/Verification. Thank you in advance Paula From LNormington at uwhealth.org Fri Mar 16 12:16:31 2018 From: LNormington at uwhealth.org (Normington Lacy) Date: Fri, 16 Mar 2018 17:16:31 +0000 Subject: [Histonet] Antibody Validation CLIA In-Reply-To: References: <001801d3bd2e$4f4b0c20$ede12460$@biopath.org> Message-ID: CAP suggests running 10 negative and 10 positive cases for non-prognostic markers. CAP required running 20 negative and 20 positive cases for prognostic markers. In the event the case volume is less than the suggested 10 and 10 cases for non-prognostic markers, the reason for that decision should be stated in the validation. Ultimately, the decision is up to your director. Lacy Normington, HTL(ASCP)CM Manager, Surgical Pathology Lab Services UW Health 600 Highland Avenue Madison, WI 53792-2472 -----Original Message----- From: Paula via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 16, 2018 8:55 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Antibody Validation CLIA WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Hello, We've been discussing about the quantity of slides to run as a validation for IHC antibodies. We are governed by CLIA, and we would like to know if there is a set number of slides to run for a particular antibody we would like to bring in-house for Validation. I think CAP requires 20 slides..? And so we are asking if there is a requirement with CLIA to run a certain number of slides, or is it up to us (the laboratory director) to decide how many slides to run for Validation/Verification. Thank you in advance Paula _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From greg.dobbin at gmail.com Fri Mar 16 12:25:05 2018 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Fri, 16 Mar 2018 14:25:05 -0300 Subject: [Histonet] Antibody Validation CLIA Message-ID: Hi Paula, Let me first say I am Canadian and my lab is not governed by CLIA or CAP. But for what it is worth, here are my thoughts... When thinking about validating antibodies for IHC you must first consider whether the antibody in question is Class-I (prognostic eg Breast markers, CD117, etc) or Class-II. Class-I require a more robust validation protocol then Class-II antibodies. Next you need to consider why the validation is needed. Is it: 1. a new Ab in your lab? 2. a new vendor for an antibody? 3. a new lot number of an existing antibody in your menu? 4. a change in protocol of an existing antibody? #'s 1 and 2 require a larger validation whereas #'s 3 and 4 should only require a minimal check (unless of course there is a drastic change in the protocol in #4). As for absolute numbers of slides for each, unless CLIA provides this information, you are left to work with your Director to find that balance between cost (ie more slides = increased cost) with confidence (how many do you need to see in order to feel confident that the stain is performing as expected). I hope this helps. Sincerely, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From liz at premierlab.com Fri Mar 16 12:42:56 2018 From: liz at premierlab.com (Liz Chlipala) Date: Fri, 16 Mar 2018 17:42:56 +0000 Subject: [Histonet] Antibody Validation CLIA In-Reply-To: References: <001801d3bd2e$4f4b0c20$ede12460$@biopath.org> Message-ID: Keep in mind like Lacy said its cases and not slides so you could place multiple cases on one slide or create a simple array using disposable biopsy punches. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Normington Lacy via Histonet Sent: Friday, March 16, 2018 11:17 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Antibody Validation CLIA CAP suggests running 10 negative and 10 positive cases for non-prognostic markers. CAP required running 20 negative and 20 positive cases for prognostic markers. In the event the case volume is less than the suggested 10 and 10 cases for non-prognostic markers, the reason for that decision should be stated in the validation. Ultimately, the decision is up to your director. Lacy Normington, HTL(ASCP)CM Manager, Surgical Pathology Lab Services UW Health 600 Highland Avenue Madison, WI 53792-2472 -----Original Message----- From: Paula via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 16, 2018 8:55 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Antibody Validation CLIA WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Hello, We've been discussing about the quantity of slides to run as a validation for IHC antibodies. We are governed by CLIA, and we would like to know if there is a set number of slides to run for a particular antibody we would like to bring in-house for Validation. I think CAP requires 20 slides..? And so we are asking if there is a requirement with CLIA to run a certain number of slides, or is it up to us (the laboratory director) to decide how many slides to run for Validation/Verification. Thank you in advance Paula _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From LNormington at uwhealth.org Fri Mar 16 13:05:10 2018 From: LNormington at uwhealth.org (Normington Lacy) Date: Fri, 16 Mar 2018 18:05:10 +0000 Subject: [Histonet] FW: Antibody Validation CLIA In-Reply-To: References: <001801d3bd2e$4f4b0c20$ede12460$@biopath.org> <45f3b3e3817a430283fe544d9d6d5618@PHX-EXCH-013.chw.edu> Message-ID: For the validation, yes you have to run negative cases. This is to verify that tissue you know should not stain, does not do so. For example, let's say you are validation H.pylori. You have 10 cases where you know the patient has H.pylori bacteria in a gastric biopsy (positive staining). You also need to run 10 samples of normal gastric tissue to verify there is no staining (negative staining). What you are thinking about is negative reagent control which is used to determine if there is non-specific staining. Lacy -----Original Message----- From: Heckford, Karen - SMMC-SF [mailto:Karen.Heckford at DignityHealth.org] Sent: Friday, March 16, 2018 12:36 PM To: Normington Lacy Subject: RE: [Histonet] Antibody Validation CLIA Do you have to run the negatives if you are using a Polymer Detection system? Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167?? karen.heckford at dignityhealth.org? Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us ?immediately by reply email.? Thank you -----Original Message----- From: Normington Lacy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 16, 2018 10:17 AM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Antibody Validation CLIA Dignityhealth.org made the following annotations --------------------------------------------------------------------- STOP, THINK, READ. This is an external email. Exercise extra caution responding to it, opening attachments and following links. --------------------------------------------------------------------- CAP suggests running 10 negative and 10 positive cases for non-prognostic markers. CAP required running 20 negative and 20 positive cases for prognostic markers. In the event the case volume is less than the suggested 10 and 10 cases for non-prognostic markers, the reason for that decision should be stated in the validation. Ultimately, the decision is up to your director. Lacy Normington, HTL(ASCP)CM Manager, Surgical Pathology Lab Services UW Health 600 Highland Avenue Madison, WI 53792-2472 -----Original Message----- From: Paula via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, March 16, 2018 8:55 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Antibody Validation CLIA WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Hello, We've been discussing about the quantity of slides to run as a validation for IHC antibodies. We are governed by CLIA, and we would like to know if there is a set number of slides to run for a particular antibody we would like to bring in-house for Validation. I think CAP requires 20 slides..? And so we are asking if there is a requirement with CLIA to run a certain number of slides, or is it up to us (the laboratory director) to decide how many slides to run for Validation/Verification. Thank you in advance Paula _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From edmartin26 at gmail.com Fri Mar 16 13:25:19 2018 From: edmartin26 at gmail.com (Eddie Martin) Date: Fri, 16 Mar 2018 14:25:19 -0400 Subject: [Histonet] Leica ST5050 immunostainer Message-ID: Dr Fleming, If you?re insistent on automation, I would suggest a used Leica Bond Max instead. The Leica Bond Max is most likely the most cost effective, and has the smallest footprint while also minimizing reagent waste compared to a Dako Flex or Ventana XT/Ultra. If you are willing to do it by hand, there a few labs that I?m aware of that use Leica?s powervision compact polymer system to stain their Melan-A?s. I?m sure it would stain a MITF just as easy. Best, Eddie M. Team lead medical technologist Histology Technical Supervisor The National Institutes of Health Clinic Center Bethesda, MD 20814 Eddie.martin at nih.gov > On Mar 16, 2018, at 9:00 AM, Matthew Fleming wrote: > > Hi, > > I have a small dermatopathology lab and would like to introduce limited > immunohistochemistry. Initially I would probably just do MITF stains with > red chromogen on a few cases per week. We could do this by hand but I've > noticed a number of ST5050 immunostainers for sale at very low prices. I > know they're quite old but we have a number of machines old or older that > continue to serve us well. But so far I haven't been able to find a manual > and am concerned about finding consumables. Leica apparently stopped > supporting this model some time ago. Is anyone out there still using it? > Anyone have a manual? Anyone like to comment as to whether it might be > usable for a limited IH operation? > > Thank you, > > Matthew Fleming, MD > Fleming Dermatopathology > Milwaukee, WI > From jennc976 at gmail.com Fri Mar 16 14:02:55 2018 From: jennc976 at gmail.com (Jennifer Clark) Date: Fri, 16 Mar 2018 15:02:55 -0400 Subject: [Histonet] Leica ST5050 immunostainer In-Reply-To: References: Message-ID: We are a small derm lab and have a Nemesis 3600 from Biocare. It will perform IHC on up to 36 slides per run and is an open platform. We also do a reagent rental through them. The doctor is happy with the quality. On Fri, Mar 16, 2018 at 2:25 PM, Eddie Martin via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Dr Fleming, > > If you?re insistent on automation, I would suggest a used Leica Bond Max > instead. The Leica Bond Max is most likely the most cost effective, and > has the smallest footprint while also minimizing reagent waste compared to > a Dako Flex or Ventana XT/Ultra. > > If you are willing to do it by hand, there a few labs that I?m aware of > that use Leica?s powervision compact polymer system to stain their > Melan-A?s. I?m sure it would stain a MITF just as easy. > > Best, > Eddie M. > > Team lead medical technologist > Histology Technical Supervisor > The National Institutes of Health > Clinic Center > Bethesda, MD 20814 > Eddie.martin at nih.gov > > > > > On Mar 16, 2018, at 9:00 AM, Matthew Fleming wrote: > > > > Hi, > > > > I have a small dermatopathology lab and would like to introduce limited > > immunohistochemistry. Initially I would probably just do MITF stains > with > > red chromogen on a few cases per week. We could do this by hand but I've > > noticed a number of ST5050 immunostainers for sale at very low prices. I > > know they're quite old but we have a number of machines old or older that > > continue to serve us well. But so far I haven't been able to find a > manual > > and am concerned about finding consumables. Leica apparently stopped > > supporting this model some time ago. Is anyone out there still using it? > > Anyone have a manual? Anyone like to comment as to whether it might be > > usable for a limited IH operation? > > > > Thank you, > > > > Matthew Fleming, MD > > Fleming Dermatopathology > > Milwaukee, WI > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tim.higgins at cddmedical.com Tue Mar 20 11:56:39 2018 From: tim.higgins at cddmedical.com (Tim Higgins) Date: Tue, 20 Mar 2018 16:56:39 +0000 Subject: [Histonet] Antibody Validation CLIA Message-ID: Cap states, "should test a minimum of 10 positive and 10 negative tissues". Does not state "cases" but "tissues", so we used a block for positive and negative from the same case. Thanks, Tim -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Friday, March 16, 2018 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 172, Issue 13 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Leica ST5050 immunostainer (Matthew Fleming) 2. Antibody Validation CLIA (Paula) ---------------------------------------------------------------------- Message: 1 Date: Fri, 16 Mar 2018 08:00:00 -0500 From: Matthew Fleming To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Leica ST5050 immunostainer Message-ID: Content-Type: text/plain; charset="UTF-8" Hi, I have a small dermatopathology lab and would like to introduce limited immunohistochemistry. Initially I would probably just do MITF stains with red chromogen on a few cases per week. We could do this by hand but I've noticed a number of ST5050 immunostainers for sale at very low prices. I know they're quite old but we have a number of machines old or older that continue to serve us well. But so far I haven't been able to find a manual and am concerned about finding consumables. Leica apparently stopped supporting this model some time ago. Is anyone out there still using it? Anyone have a manual? Anyone like to comment as to whether it might be usable for a limited IH operation? Thank you, Matthew Fleming, MD Fleming Dermatopathology Milwaukee, WI ------------------------------ Message: 2 Date: Fri, 16 Mar 2018 06:54:30 -0700 From: "Paula" To: Subject: [Histonet] Antibody Validation CLIA Message-ID: <001801d3bd2e$4f4b0c20$ede12460$@biopath.org> Content-Type: text/plain; charset="us-ascii" Hello, We've been discussing about the quantity of slides to run as a validation for IHC antibodies. We are governed by CLIA, and we would like to know if there is a set number of slides to run for a particular antibody we would like to bring in-house for Validation. I think CAP requires 20 slides..? And so we are asking if there is a requirement with CLIA to run a certain number of slides, or is it up to us (the laboratory director) to decide how many slides to run for Validation/Verification. Thank you in advance Paula ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 172, Issue 13 ***************************************** From tbraud at holyredeemer.com Tue Mar 20 12:54:27 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 20 Mar 2018 17:54:27 +0000 Subject: [Histonet] IHC validation Message-ID: <48E053DDF6CE074DB6A7414BA05403F8C1B89136@HRHEX02-HOS.holyredeemer.local> Just another note: You can order unstained tissue microarrays with the prerequisite number of cases, both positive and negative, and stain your validation all on one slide. I've done this for years and for 3 different validations of entire IHC platform changes, ranging from 40 to over 100 antibodies each time. Saves time and money. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Message: 2 Date: Fri, 16 Mar 2018 06:54:30 -0700 From: "Paula" Subject: [Histonet] Antibody Validation CLIA Hello, We've been discussing about the quantity of slides to run as a validation for IHC antibodies. We are governed by CLIA, and we would like to know if there is a set number of slides to run for a particular antibody we would like to bring in-house for Validation. I think CAP requires 20 slides..? And so we are asking if there is a requirement with CLIA to run a certain number of slides, or is it up to us (the laboratory director) to decide how many slides to run for Validation/Verification. Thank you in advance Paula From cforster at umn.edu Tue Mar 20 13:27:19 2018 From: cforster at umn.edu (Colleen Forster) Date: Tue, 20 Mar 2018 13:27:19 -0500 Subject: [Histonet] IHC validation In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8C1B89136@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8C1B89136@HRHEX02-HOS.holyredeemer.local> Message-ID: I agree Terry, The TMA slide is a very economical and powerful way to validate with minimal slides needed. Colleen Forster On Tue, Mar 20, 2018 at 12:54 PM, Terri Braud via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Just another note: You can order unstained tissue microarrays with the > prerequisite number of cases, both positive and negative, and stain your > validation all on one slide. I've done this for years and for 3 different > validations of entire IHC platform changes, ranging from 40 to over 100 > antibodies each time. Saves time and money. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Message: 2 > Date: Fri, 16 Mar 2018 06:54:30 -0700 > From: "Paula" > Subject: [Histonet] Antibody Validation CLIA > > Hello, > We've been discussing about the quantity of slides to run as a validation > for IHC antibodies. We are governed by CLIA, and we would like to know if > there is a set number of slides to run for a particular antibody we would > like to bring in-house for Validation. I think CAP requires 20 slides..? > And so we are asking if there is a requirement with CLIA to run a certain > number of slides, or is it up to us (the laboratory director) to decide how > many slides to run for Validation/Verification. > Thank you in advance > Paula > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From BZIMMERM at augusta.edu Tue Mar 20 15:02:47 2018 From: BZIMMERM at augusta.edu (Zimmerman, Billie) Date: Tue, 20 Mar 2018 20:02:47 +0000 Subject: [Histonet] Georgia Society for Histotechnology HISTOPALOOZA April 20 - 22 Message-ID: This year our meeting will be held at Callaway Gardens Lodge, Pine Mountain, Georgia. Remember we offer free membership. The all-inclusive registration fee is $135, which is a steal these days for CEUs. Jack Ratliff will be back this year as a speaker. Woohoo, can't wait to catch up with him, so he can ask me where my phone is. Please google the GA Society for histotechnology for more information. Billie Zimmerman MT(ASCP)QIHC From modz9636 at gmail.com Tue Mar 20 16:34:20 2018 From: modz9636 at gmail.com (M.O.) Date: Tue, 20 Mar 2018 14:34:20 -0700 Subject: [Histonet] cryosections for LCM not staying on slides Message-ID: Hello Histonet, I am just starting a project using LCM for RNA extraction. So far, our tissues haven't stayed on the slides very well. We are using the Acrturus LCM staining kit. We've taken meniscus at 10, 20, 30, and 40uM sections and started the staining process with (10mins at room temp) and without drying. We want to make sure not to degrade any RNA. Some tissues stay on the slides through to the xylenes, but when we air dry for 5mins, they begin to shrink and pick up from the slides. Our next trial will be with thinner sections, and also trying a short PFA fixation for 10mins. Do you have any suggestions for keeping these sections on the slides and not causing issues with RNA? Thank you for any advice you may have. Sincerely, Merissa From relia1 at earthlink.net Wed Mar 21 09:15:30 2018 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 21 Mar 2018 10:15:30 -0400 Subject: [Histonet] Exciting Opportunity to RUN YOUR OWN BRAND NEW LAB in SoCAL IT's a RELIA Exclusive! Message-ID: <00b501d3c11f$117762a0$346627e0$@earthlink.net> Hi Histonetters! Happy Hump Day! I have an exciting announcement to make!! I am currently working with a new GI lab in Pomona, CA that is seeking a Histotech/Supervisor to run their own lab. You will be responsible for processing tissue and handling all QA, QC, administrative and compliance duties. You will have the unique opportunity to work one on one with one of the best most long standing practices in the area. They are truly a great team to work with and the lab will be ALL YOURS!!! This is a RELIA EXCLUSIVE!!! For more info contact Pam Barker at relia1 at earthlink.net to schedule a time to talk or if you want to chat asap please call my cell at 407-353-5070. And if this isn?t the one for you but you know someone else who might be interested please pass along the info! If I hire them you will earn a referral fee. Have a great day!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From litepath2000 at yahoo.com Wed Mar 21 09:57:54 2018 From: litepath2000 at yahoo.com (LitePath) Date: Wed, 21 Mar 2018 14:57:54 +0000 (UTC) Subject: [Histonet] Exciting Opportunity to RUN YOUR OWN BRAND NEW LAB in SoCAL IT's a RELIA Exclusive! In-Reply-To: <00b501d3c11f$117762a0$346627e0$@earthlink.net> References: <00b501d3c11f$117762a0$346627e0$@earthlink.net> Message-ID: <1857191101.4604754.1521644274866@mail.yahoo.com> Hi PamIf i may ask, can you to please post any opportunities directly to our message board?? Its getting difficult for me to keep pace with all the mail.? Its pretty straight forward, just send your message to: nyshs1972@ yahoogroups.com Feel free to post as frequently as you want. While this message board was originally created to communicate with New York State Society members, we have subscribers from across the region.. I will send you an invite from same address above to the message board so that you can see and post directly.ThanksLuis On Wednesday, March 21, 2018, 10:33:02 AM EDT, Pam Barker via Histonet wrote: Hi Histonetters! Happy Hump Day! I have an exciting announcement to make!! I am currently working with a new GI lab in Pomona, CA that is seeking a Histotech/Supervisor to run their own lab.? You will be responsible for processing tissue and handling all QA, QC, administrative and compliance duties.? You will have the unique opportunity to work one on one with one of the best most long standing practices in the area.? They are truly a great team to work with and the lab will be ALL YOURS!!!? This is a RELIA EXCLUSIVE!!! For more info contact Pam Barker at relia1 at earthlink.net to schedule a time to talk or if you want to chat asap please call my cell at 407-353-5070. And if this isn?t the one for you but you know someone else who might be interested please pass along the info!? If I hire them you will earn a referral fee. Have a great day!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ana.Maluenda at baker.edu.au Thu Mar 22 01:30:00 2018 From: Ana.Maluenda at baker.edu.au (Ana Maluenda) Date: Thu, 22 Mar 2018 06:30:00 +0000 Subject: [Histonet] IHC validation In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8C1B89136@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8C1B89136@HRHEX02-HOS.holyredeemer.local> Message-ID: Hi Terri, This is actually a very valid note. This has been in my mind for years, but never had the chance to put in action in a research environment (I always try to bring to the research field the efficiency and money saving strategies we use in diagnostics). Where do you usually order your unstained slides from? Much appreciated for the advice. Kind regards, Ana Ana Maluenda Research Assistant Atherothrombosis and Vascular Biology Laboratory Baker Heart and Diabetes Institute 75 Commercial Road, Melbourne VIC 3004 P (03) 8532 1359 E Ana.Maluenda at baker.edu.au W www.baker.edu.au -----Original Message----- From: Terri Braud [mailto:tbraud at holyredeemer.com] Sent: Wednesday, 21 March 2018 4:54 AM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] IHC validation Just another note: You can order unstained tissue microarrays with the prerequisite number of cases, both positive and negative, and stain your validation all on one slide. I've done this for years and for 3 different validations of entire IHC platform changes, ranging from 40 to over 100 antibodies each time. Saves time and money. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Message: 2 Date: Fri, 16 Mar 2018 06:54:30 -0700 From: "Paula" Subject: [Histonet] Antibody Validation CLIA Hello, We've been discussing about the quantity of slides to run as a validation for IHC antibodies. We are governed by CLIA, and we would like to know if there is a set number of slides to run for a particular antibody we would like to bring in-house for Validation. I think CAP requires 20 slides..? And so we are asking if there is a requirement with CLIA to run a certain number of slides, or is it up to us (the laboratory director) to decide how many slides to run for Validation/Verification. Thank you in advance Paula Protecting your privacy is important to us. The Baker Heart and Diabetes Institute will handle your information in accordance with the Privacy Act 1988 (Cth) and its Privacy Policy which is available at www.baker.edu.au or on request by contacting privacy at baker.edu.au or by calling 1800 838 498. The Privacy Policy also explains how you can access and correct your personal information, or make a complaint about a breach of the Australian Privacy Principles. bidipp2014.0.1a -- Message protected by MailGuard: e-mail anti-virus, anti-spam and content filtering.http://www.mailguard.com.au/mg From Scott.Hall at nationwidechildrens.org Thu Mar 22 10:32:22 2018 From: Scott.Hall at nationwidechildrens.org (Hall, Scott) Date: Thu, 22 Mar 2018 15:32:22 +0000 Subject: [Histonet] Leica CV5030 Message-ID: What does everyone do with their Xylene bath at the end of the day. Cover it and turn off or cover it and take it out? Just wondering Scott Hall Senior Histotechnician Nationwide Children's Hospital From mjdessoye at commonwealthhealth.net Thu Mar 22 10:59:49 2018 From: mjdessoye at commonwealthhealth.net (Dessoye, Michael) Date: Thu, 22 Mar 2018 15:59:49 +0000 Subject: [Histonet] IHC validation In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8C1B89136@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8C1B89136@HRHEX02-HOS.holyredeemer.local> Message-ID: Can anyone recommend a vendor that they've had good luck with for TMA slides? Michael J. Dessoye, M.S.?|?Histology/Toxicology/Special Chemistry Supervisor?|?Commonwealth Health Laboratory Services |?mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 -----Original Message----- From: Terri Braud [mailto:tbraud at holyredeemer.com] Sent: Tuesday, March 20, 2018 1:54 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] IHC validation Just another note: You can order unstained tissue microarrays with the prerequisite number of cases, both positive and negative, and stain your validation all on one slide. I've done this for years and for 3 different validations of entire IHC platform changes, ranging from 40 to over 100 antibodies each time. Saves time and money. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Message: 2 Date: Fri, 16 Mar 2018 06:54:30 -0700 From: "Paula" Subject: [Histonet] Antibody Validation CLIA Hello, We've been discussing about the quantity of slides to run as a validation for IHC antibodies. We are governed by CLIA, and we would like to know if there is a set number of slides to run for a particular antibody we would like to bring in-house for Validation. I think CAP requires 20 slides..? And so we are asking if there is a requirement with CLIA to run a certain number of slides, or is it up to us (the laboratory director) to decide how many slides to run for Validation/Verification. Thank you in advance Paula From Timothy.Morken at ucsf.edu Thu Mar 22 11:12:18 2018 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 22 Mar 2018 16:12:18 +0000 Subject: [Histonet] IHC validation In-Reply-To: References: <48E053DDF6CE074DB6A7414BA05403F8C1B89136@HRHEX02-HOS.holyredeemer.local> Message-ID: I've used hundreds of TMA's from Pantomics and Biochain with great results. https://pantomics.com/ https://www.biochain.com/ Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Dessoye, Michael via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, March 22, 2018 9:00 AM To: Terri Braud; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] IHC validation Can anyone recommend a vendor that they've had good luck with for TMA slides? Michael J. Dessoye, M.S.?|?Histology/Toxicology/Special Chemistry Supervisor?|?Commonwealth Health Laboratory Services |?mjdessoye at commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 -----Original Message----- From: Terri Braud [mailto:tbraud at holyredeemer.com] Sent: Tuesday, March 20, 2018 1:54 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] IHC validation Just another note: You can order unstained tissue microarrays with the prerequisite number of cases, both positive and negative, and stain your validation all on one slide. I've done this for years and for 3 different validations of entire IHC platform changes, ranging from 40 to over 100 antibodies each time. Saves time and money. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Message: 2 Date: Fri, 16 Mar 2018 06:54:30 -0700 From: "Paula" Subject: [Histonet] Antibody Validation CLIA Hello, We've been discussing about the quantity of slides to run as a validation for IHC antibodies. We are governed by CLIA, and we would like to know if there is a set number of slides to run for a particular antibody we would like to bring in-house for Validation. I think CAP requires 20 slides..? And so we are asking if there is a requirement with CLIA to run a certain number of slides, or is it up to us (the laboratory director) to decide how many slides to run for Validation/Verification. Thank you in advance Paula _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From allanw at biomax.us Thu Mar 22 13:01:50 2018 From: allanw at biomax.us (Allan Wang) Date: Thu, 22 Mar 2018 14:01:50 -0400 Subject: [Histonet] IHC validation In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8C1B89136@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8C1B89136@HRHEX02-HOS.holyredeemer.local> Message-ID: How are labs validating for rarer biomarkers like ALK, ROS1, or MMR loss? Following the guideline of 10 positive cases may be difficult. I've seen other companies with control slides just with a few engineered cell lines as positive and negative controls. Is that enough for validation alone? We are creating TMAs for validation of ALK and MMR loss (MLH1, PMS2, MSH6, MSH2) so I am interested in knowing how many cases are desired. Allan Allan Wang Lab Manager US Biomax On Tue, Mar 20, 2018 at 1:54 PM, Terri Braud via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Just another note: You can order unstained tissue microarrays with the > prerequisite number of cases, both positive and negative, and stain your > validation all on one slide. I've done this for years and for 3 different > validations of entire IHC platform changes, ranging from 40 to over 100 > antibodies each time. Saves time and money. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Message: 2 > Date: Fri, 16 Mar 2018 06:54:30 -0700 > From: "Paula" > Subject: [Histonet] Antibody Validation CLIA > > Hello, > We've been discussing about the quantity of slides to run as a validation > for IHC antibodies. We are governed by CLIA, and we would like to know if > there is a set number of slides to run for a particular antibody we would > like to bring in-house for Validation. I think CAP requires 20 slides..? > And so we are asking if there is a requirement with CLIA to run a certain > number of slides, or is it up to us (the laboratory director) to decide how > many slides to run for Validation/Verification. > Thank you in advance > Paula > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Richard.Cartun at hhchealth.org Thu Mar 22 16:25:20 2018 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Thu, 22 Mar 2018 21:25:20 +0000 Subject: [Histonet] COnsult Reporting Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EA8A0003E@HHCEXCHMB03.hhcsystem.org> I know I asked this question before, but I can't find my notes. If you provide a diagnostic consultation on pathology slides, do you need a separate report with a new "Date-of-Service" (the date that the consult is requested) for reporting and billing purposes? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From fpearsa at clemson.edu Fri Mar 23 09:09:56 2018 From: fpearsa at clemson.edu (Frances Pearsall) Date: Fri, 23 Mar 2018 14:09:56 +0000 Subject: [Histonet] Congo Red Message-ID: Can anyone tell me what kind (or size) glass filter to use to perform Puchtlers Congo Red? And are the filters re-useable? Thank you, Fran Pearsall From rjbuesa at yahoo.com Fri Mar 23 10:08:08 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Fri, 23 Mar 2018 15:08:08 +0000 (UTC) Subject: [Histonet] Congo Red In-Reply-To: References: Message-ID: <104416684.5937844.1521817688939@mail.yahoo.com> You will need a polarizing filter. The size is just the necessary to cover your light source.?Ren? On Friday, March 23, 2018 10:27 AM, Frances Pearsall via Histonet wrote: Can anyone tell me what kind (or size)? glass filter to use to perform Puchtlers Congo Red? And are the filters re-useable? Thank you, Fran Pearsall _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From teri.johnson at navigatebp.com Fri Mar 23 10:17:30 2018 From: teri.johnson at navigatebp.com (Johnson, Teri) Date: Fri, 23 Mar 2018 15:17:30 +0000 Subject: [Histonet] Microtome maintenance Message-ID: <3dc9c03f24004257881a346966c5f2e9@DM5PR6207MB0074.023d.mgd.msft.net> Hi fellow histonetters, I am interested in finding out what those of you in CAP/CLIA labs are doing for routine maintenance of your microtomes, especially for smaller volume labs. Do you have service contracts set up? Or regular (annual or every other year) maintenance schedules? The cost of "periodic inspection" is pretty significant. Thanks in advance and Happy Friday! Teri Johnson, HT(ASCP)QIHC Manager, Histopathology and IHC T +1 760 602 1402 (new phone number) teri.johnson at navigatebp.com Navigate BioPharma Services, Inc. A Novartis Subsidiary 1890 Rutherford Rd., Carlsbad, CA 92008 USA From tfraser at olympicmedical.org Fri Mar 23 10:39:36 2018 From: tfraser at olympicmedical.org (Tasha Fraser) Date: Fri, 23 Mar 2018 15:39:36 +0000 Subject: [Histonet] Telepathology Message-ID: Can non-grossing Histotech's gross and perform frozen sections via telepathology? The pathologist would be at a different location but guide the histotech to cut tissue via an Elmo Camera. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. From tfraser at olympicmedical.org Fri Mar 23 12:23:42 2018 From: tfraser at olympicmedical.org (Tasha Fraser) Date: Fri, 23 Mar 2018 17:23:42 +0000 Subject: [Histonet] Telepathology Message-ID: <15fbeafe125f4d489f58e802102a7dd9@olympicmedical.org> Can non-grossing Histotech's gross and perform frozen sections and daily grossing via telepathology? The pathologist would be at a different location but guide the histotech to cut tissue via an Elmo Camera. Any and all comments would be appreciated. Tasha Fraser, HT (ASCP) Olympic Medical Center Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. From Karen.Heckford at DignityHealth.org Fri Mar 23 14:49:14 2018 From: Karen.Heckford at DignityHealth.org (Heckford, Karen - SMMC-SF) Date: Fri, 23 Mar 2018 19:49:14 +0000 Subject: [Histonet] Assistant Message-ID: Good Afternoon, We are looking for a per diem person for our Pathology and cytology department. This person will be picking up specimens, doing accessioning, making cassettes, maintaining the grossing room, tissue dumping, assist with FNA's, prep cell buttons and do cytospins and staining. Also go to surgery and do specimen pick up and maintain the Frozen section room. I am guessing this person would be just a lab assistant dedicated to Pathology? Not sure what the correct Job title should be. This person would cover for vacations, sick time etc. Also this person may get to dabble in some Histology stuff as well under the supervision of the Tech if they want to get more hours. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckford at dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you From jcox90 at yahoo.com Fri Mar 23 15:56:57 2018 From: jcox90 at yahoo.com (Jill Cox) Date: Fri, 23 Mar 2018 13:56:57 -0700 Subject: [Histonet] Surepath or thinprep Message-ID: Hi Netters, Those of you that perform cytology's which one of these machines do you prefer,surePath or thin prep? Any pros and cons of either? I haven?t done cytology in many years and I?m sure everything has changed as I used to do it manually. Any help would be much appreciated! Thank you all and have a great weekend! Sent from my iPad From rsrichmond at gmail.com Fri Mar 23 18:44:08 2018 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 23 Mar 2018 19:44:08 -0400 Subject: [Histonet] Congo Red Message-ID: Clemson Tiger Fran Pearsall asks: >>Can anyone tell me what kind (or size) glass filter to use to perform Puchtler's Congo Red? And are the filters re-useable?<< I suppose you refer to polarization, necessary to examine a Congo red stain for amyloid. Your microscope needs a polarizer, preferably a full wave plate (first order plate) system. Many pathologists don't have these on their microscopes. Your urine lab across the hall has one, though you'll probably have to spend five minutes cleaning and aligning the microscope. You should know how to evaluate your positive control, particularly if your pathologist doesn't know how. The most common positive control available in a surgical pathology lab is a medullary carcinoma of the thyroid. Most (but not all) of these contain some amyloid. I've asked this question many times over the years and never received a glimmer of an answer: amyloid is rather easily produced experimentally in mice. Is this material commercially available, and is it permitted for use as an amyloid control? Bob Richmond Samurai Pathologist Maryville TN From lori.garcia at medtronic.com Fri Mar 23 20:26:22 2018 From: lori.garcia at medtronic.com (Garcia, Lori, M.Sc.) Date: Sat, 24 Mar 2018 01:26:22 +0000 Subject: [Histonet] Leica CM3050S available Message-ID: Hi everyone, After building and running a histology lab for over 13 years, my company has decided to shut down internal processing and convert the space. I have a Leica CM3050S cryostat that is in pristine condition and would be happy to donate to someone in need. The only cost will be the shipping charges. I can also send all accessories that we have. Thanks, Lori Garcia [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From carl.hobbs at kcl.ac.uk Sat Mar 24 12:52:51 2018 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sat, 24 Mar 2018 17:52:51 +0000 Subject: [Histonet] Congo Red Message-ID: That's a good point, Bob (mouse Amyloid controls for human sections) The amyloid produced by these various mouse models (TASTPM, TGs) is human amyloid....as you know. I suspect that it wouldn't be accepted as a positive control ( I don't know) because it isn't pathologically produced It is genetically induced ( as you know) I have posted images of IHC- and Congo red-positivity ( the latter also under fluorescence microscope) of amyloid in mouse brain here ( Histonet Images archive) Respectfully Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From boznpl at aol.com Sat Mar 24 16:23:37 2018 From: boznpl at aol.com (Laurie Colbert) Date: Sat, 24 Mar 2018 17:23:37 -0400 Subject: [Histonet] Per diem histotech needed for nights, Los Angeles area Message-ID: <16259e53957-c85-143c0@webjas-vae035.srv.aolmail.net> PATH MD is looking for a motivated and reliable per diem histotech to work occasional nights starting at 9:00 pm.? The job would consist mainly of embedding and some cutting, but knowledge in all aspects of histology preferred.? Must be ASCP certified and have at least one year of histology experience. Competitive salary offered. Please forward resumes to Laurie Redmond at boznpl at aol.com From jqb7 at cdc.gov Sat Mar 24 16:44:38 2018 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Sat, 24 Mar 2018 21:44:38 +0000 Subject: [Histonet] Fw: Re: Leica CV5030 In-Reply-To: <581f7d46-da66-4d28-a0dc-ed1cf5e68246@EXPP-EXCH-CHAM2.cdc.gov> References: <2598ad608e97498e949020146959f3db@cdc.gov>, <581f7d46-da66-4d28-a0dc-ed1cf5e68246@EXPP-EXCH-CHAM2.cdc.gov> Message-ID: ________________________________ From: Microsoft Outlook Sent: Saturday, March 24, 2018 12:39 PM To: Hall, Scott Subject: Delivery delayed:Re: Leica CV5030 Delivery is delayed to these recipients or groups: Hall, Scott (Scott.Hall at nationwidechildrens.org) Subject: Re: Leica CV5030 This message hasn't been delivered yet. Delivery will continue to be attempted. The server will keep trying to deliver this message for the next 1 days, 19 hours and 55 minutes. You'll be notified if the message can't be delivered by that time. Diagnostic information for administrators: Generating server: EXPP-EXCH-CHAM2.cdc.gov Receiving server: cluster5out.us.messagelabs.com Scott.Hall at nationwidechildrens.org Server at cluster5out.us.messagelabs.com returned '400 4.4.7 Message delayed' 3/24/2018 4:28:46 PM - Server at cluster5out.us.messagelabs.com returned '451 4.4.0 DNS query failed. The error was: DNS query failed with error ServerFailure -> DnsQueryFailed: ServerFailure' Original message headers: Received: from EXPP-EXCH-CLFT1.cdc.gov (158.111.54.111) by EXPP-EXCH-CHAM2.cdc.gov (158.111.190.242) with Microsoft SMTP Server (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_CBC_SHA256_P256) id 15.1.1415.2; Sat, 24 Mar 2018 08:35:02 -0400 Received: from EXPP-EXCH-CLFT1.cdc.gov ([158.111.57.31]) by EXPP-EXCH-CLFT1.cdc.gov ([158.111.57.31]) with mapi id 15.01.1415.002; Sat, 24 Mar 2018 08:35:02 -0400 From: "Sanders, Jeanine (CDC/OID/NCEZID)" To: "Hall, Scott" Subject: Re: Leica CV5030 Thread-Topic: Leica CV5030 Thread-Index: AdPB8s+ymBZURHa/QoqAFgRLFueCIQBeadUb Date: Sat, 24 Mar 2018 12:35:02 +0000 Message-ID: <2598ad608e97498e949020146959f3db at cdc.gov> References: In-Reply-To: Accept-Language: en-US Content-Language: en-US X-MS-Has-Attach: X-MS-TNEF-Correlator: x-originating-ip: [158.111.54.75] Content-Type: multipart/alternative; boundary="_000_2598ad608e97498e949020146959f3dbcdcgov_" MIME-Version: 1.0 From histo at pathlab.us Mon Mar 26 06:41:39 2018 From: histo at pathlab.us (Histology) Date: Mon, 26 Mar 2018 11:41:39 +0000 Subject: [Histonet] Silver waste Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD8206E253A3@vdc01.domain.local> Hi All. Happy Monday! Would anyone share how they collect, store, and dispose of silver waste from manual special stains such as GMS, Melanin Fontana-Masson, and Calcium Von Kossa? Thanks in advance! From scrochiere at nedlc.com Mon Mar 26 10:17:19 2018 From: scrochiere at nedlc.com (Steven Crochiere) Date: Mon, 26 Mar 2018 11:17:19 -0400 Subject: [Histonet] PRN opening Message-ID: <000001d3c515$889d6d80$99d84880$@nedlc.com> New England dermatology in Springfield, MA has an immediate opening for an experienced, per-diem HT in our dermatopathology and Mohs department . Responsibilities will include embedding, microtomy and staining. Mohs experience is a plus. FT potential. Contact: Terry Molnar, Human Resources New England dermatology and Laser Center 3455 Main St Springfield, MA 01107 (413)733-9600 x 319 From fpearsa at clemson.edu Mon Mar 26 14:28:18 2018 From: fpearsa at clemson.edu (Frances Pearsall) Date: Mon, 26 Mar 2018 19:28:18 +0000 Subject: [Histonet] Congo Red- Glass wool Message-ID: Can anyone tell me what kind and/or size glass wool do you use to for filtering the Working Congo Red solution right before use? From what I've read, it recommends not to use regular filter paper. Thank you so much, Fran Pearsall, ASCP From Timothy.Morken at ucsf.edu Mon Mar 26 14:49:59 2018 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Mon, 26 Mar 2018 19:49:59 +0000 Subject: [Histonet] Congo Red- Glass wool In-Reply-To: References: Message-ID: Fran, I've never heard of that. The books I looked at just say "filter" with no specific filter recommendations. For Sudan Black and Congo red we use Whatman "Reeve Angel 802" (Pleated). It has a 15um pore size which is "coarse." Fisher catalog number 09-901A Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Frances Pearsall via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, March 26, 2018 12:28 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Congo Red- Glass wool Can anyone tell me what kind and/or size glass wool do you use to for filtering the Working Congo Red solution right before use? From what I've read, it recommends not to use regular filter paper. Thank you so much, Fran Pearsall, ASCP _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Mon Mar 26 16:05:08 2018 From: rsrichmond at gmail.com (Bob Richmond) Date: Mon, 26 Mar 2018 17:05:08 -0400 Subject: [Histonet] Congo Red Message-ID: Carl Hobbs FIBMS, Histology and Imaging Manager at Kings College in London replies: >>That's a good point, Bob (mouse amyloid controls for human sections). The amyloid produced by the the various mouse models (TASTPM, TGs) is human amyloid... as you know. I suspect that it wouldn't be accepted as a positive control (I don't know) because it isn't pathologically produced. It is genetically induced as you know).<< There are more then ten structurally unrelated proteins capable of arranging into clinically significant amyloid in people. They have slightly different tinctorial properties, and of course react differently to immunohistochemicaly studies. I don't know of any regulatory agency that has rules about amyloid controls. The mouse models of amyloidosis involve injection of casein (or diazotized casein). I haven't read anything about these for close to fifty years, and there may be better methods now. I'd want an animal amyloid that was either AA or AL type amyloid. Bob Richmond Samurai Pathologist Maryville, Tennessee From melissa at alliedsearchpartners.com Tue Mar 27 08:33:56 2018 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Tue, 27 Mar 2018 13:33:56 +0000 Subject: [Histonet] MOHS Histology Tech Job in Chicago-Permanent/Full Time Message-ID: Hello Histonet, I have a full time/permanent job opening for a MOHS Histotech in Chicago, IL. Please reach out to me directly for more details and to be considered. Melissa Owens, CHP (ASA) President, Laboratory Staffing Allied Search Partners T: 888.388.7571 ext. 102 Direct Line: 407.413.9117 F: 888.388.7572 Melissa at alliedsearchpartners.com From koesslkl1 at gmail.com Tue Mar 27 15:25:57 2018 From: koesslkl1 at gmail.com (Karl Koessler) Date: Tue, 27 Mar 2018 20:25:57 +0000 Subject: [Histonet] Stain for specific nerve types in sacral spinal cord Message-ID: Does anyone know of a stain or marker I can use to differentiate sympathetic from parasympathetic in sacral spinal cord sections? Thanks in advance. From cforster at umn.edu Tue Mar 27 15:58:59 2018 From: cforster at umn.edu (Colleen Forster) Date: Tue, 27 Mar 2018 15:58:59 -0500 Subject: [Histonet] Stain for specific nerve types in sacral spinal cord In-Reply-To: References: Message-ID: Following...would like to see the answer. Colleen Forster On Tue, Mar 27, 2018 at 3:25 PM, Karl Koessler via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Does anyone know of a stain or marker I can use to differentiate > sympathetic from parasympathetic in sacral spinal cord sections? > > Thanks in advance. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ccrowder25 at gmail.com Tue Mar 27 21:21:30 2018 From: ccrowder25 at gmail.com (Cheryl Crowder) Date: Tue, 27 Mar 2018 21:21:30 -0500 Subject: [Histonet] Congo Red filters Message-ID: Fran - You can use Whatman #1 filter paper but it is a lot cheaper and just as good to use 2 coffee filters. They are the right size for filtering stains. Cheryl Crowder From boznpl at aol.com Wed Mar 28 09:06:50 2018 From: boznpl at aol.com (Laurie Colbert) Date: Wed, 28 Mar 2018 10:06:50 -0400 Subject: [Histonet] Supervisor and per diem histotech positions available in Los Angeles Message-ID: <1626ceebe9c-1dda-8201@webjas-vab091.srv.aolmail.net> Path MD, a state of the art private pathology lab located in West Hollywood, CA, is looking for a full time Histology supervisor to join our team.? The position is available immediately, Monday-Friday, 4:00 am to 12:30 pm.? HT(ASCP) and bachelors degree required, and HTL(ASCP) preferred.? At least 5 years supervisory experience is required, as well as knowledge of IHC and CAP regulations. Must be reliable and self-motivated.? Path MD offers medical, dental and vision benefits as well as a 401(k) plan with matching benefits.? Relocation assistance a possibility. Please submit resume to David Costanzo, Director of Operations at:? dcostanzo at pathmdlabs.com Path MD is also looking for a per diem histotech to work occasional nights starting at 9:00 pm.? Must be HT(ASCP) with at least one year of experience in routine histology and IHC experience is preferred.? Please send resume to Laurie Redmond at: boznpl at aol.com From Stefanie.Harris at crl.com Wed Mar 28 10:36:59 2018 From: Stefanie.Harris at crl.com (Harris, Stefanie) Date: Wed, 28 Mar 2018 15:36:59 +0000 Subject: [Histonet] Slide labels falling off- Slidemate AS Message-ID: Hello all, I am hoping someone out there can help. We have been having issues with some of the labels coming off during staining and after. It seems to be random so I can't tell if there is a problem with the slides or the Slidemate AS. There are days that it will be half the slides but other days where it will be none. Sometimes the labels peel off and float away during staining and other times labels rub off when handling but only after staining. We are using Globe Scientific Slides, and have been for over 2 years with no issues until December. I have changed out the ribbon on the slidemate and the problem is still sporadic. Oven time drying time does not seem to be a factor. We have not made any reagent changes for the staining process to explain the issue either. Has anyone else seen anything like this? Any slide suggestions to try out? Stefanie Harris Research Assistant, Histology Research Animal Diagnostic Services | Charles River 261 Ballardvale St., Wilmington, MA 01887 P: 781.222.6592 | F: 978.658.7698 stefanie.harris at crl.com | www.criver.com LinkedIn | Twitter | Facebook | Eureka Confidentiality Notice: The information contained in this email and any attachments are confidential and may contain privileged and/or proprietary information. This email is intended for the use of the addressee only. If you are not the intended recipient, you are strictly prohibited from copying, distributing or using this email or the information contained in it. If you received this email in error, please notify the sender immediately by email or telephone, and destroy any copies, electronic, paper or otherwise. From boznpl at aol.com Wed Mar 28 11:01:36 2018 From: boznpl at aol.com (Laurie Colbert) Date: Wed, 28 Mar 2018 12:01:36 -0400 Subject: [Histonet] Slide labels falling off- Slidemate AS In-Reply-To: References: Message-ID: <1626d57d898-17a0-68b8@webjas-vaa167.srv.aolmail.net> Stefanie, When you say "labels" are? you talking about paper labels or the print on the slides from the Slidemate?? I will assume that you mean the print, and yes, I have had the problem in the past where the print came off during staining and/or you could literally wipe the print off with a kimwipe or your finger.? In our case, it was the slides - even though it was the same slide we had been using for years.? This was happening with a certain lot number.? Is this happening with just one lot number?? Maybe you can try and get a different lot number or try a totally different slide (different manufacturer) to narrow down the issue. Good luck! Laurie -----Original Message----- From: Harris, Stefanie via Histonet To: histonet Sent: Wed, Mar 28, 2018 8:51 am Subject: [Histonet] Slide labels falling off- Slidemate AS Hello all, I am hoping someone out there can help. We have been having issues with some of the labels coming off during staining and after. It seems to be random so I can't tell if there is a problem with the slides or the Slidemate AS. There are days that it will be half the slides but other days where it will be none. Sometimes the labels peel off and float away during staining and other times labels rub off when handling but only after staining. We are using Globe Scientific Slides, and have been for over 2 years with no issues until December. I have changed out the ribbon on the slidemate and the problem is still sporadic. Oven time drying time does not seem to be a factor. We have not made any reagent changes for the staining process to explain the issue either. Has anyone else seen anything like this? Any slide suggestions to try out? Stefanie Harris Research Assistant, Histology Research Animal Diagnostic Services | Charles River 261 Ballardvale St., Wilmington, MA 01887 P: 781.222.6592 | F: 978.658.7698 stefanie.harris at crl.com | www.criver.com LinkedIn | Twitter | Facebook | Eureka Confidentiality Notice: The information contained in this email and any attachments are confidential and may contain privileged and/or proprietary information. This email is intended for the use of the addressee only. If you are not the intended recipient, you are strictly prohibited from copying, distributing or using this email or the information contained in it. If you received this email in error, please notify the sender immediately by email or telephone, and destroy any copies, electronic, paper or otherwise. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stefanie.Harris at crl.com Wed Mar 28 11:17:11 2018 From: Stefanie.Harris at crl.com (Harris, Stefanie) Date: Wed, 28 Mar 2018 16:17:11 +0000 Subject: [Histonet] [External]: Re: Slide labels falling off- Slidemate AS In-Reply-To: <1626d57d898-17a0-68b8@webjas-vaa167.srv.aolmail.net> References: , <1626d57d898-17a0-68b8@webjas-vaa167.srv.aolmail.net> Message-ID: Hi Laurie, Thanks for you response. I am talking about the print from the slidemate, not paper labels. We have tried different lots and it seems to be a bunch of them. There was one lot that was really bad in December and we thought that would be the end of the problem. We contacted the vendor and they said they hadn't heard of any other tissues and have made no changes. We can see the variability in some of the white frosted area so there are clearly differences in the slide. When we contacted the 2nd time about the ongoing issue, they said that the slides were not tested for use on that printer but they would look into it. I have a couple different brands (Fisher & Statlab) that I am hoping to test out but I am open other suggestions as well. Stefanie Harris Research Assistant, Histology Research Animal Diagnostic Services | Charles River 261 Ballardvale St., Wilmington, MA 01887 P: 781.222.6592 | F: 978.658.7698 stefanie.harris at crl.com | www.criver.com LinkedIn | Twitter | Facebook | Eureka Confidentiality Notice: The information contained in this email and any attachments are confidential and may contain privileged and/or proprietary information. This email is intended for the use of the addressee only. If you are not the intended recipient, you are strictly prohibited from copying, distributing or using this email or the information contained in it. If you received this email in error, please notify the sender immediately by email or telephone, and destroy any copies, electronic, paper or otherwise. ________________________________ From: Laurie Colbert Sent: Wednesday, March 28, 2018 12:01:36 PM To: Harris, Stefanie; histonet at lists.utsouthwestern.edu Subject: [External]: Re: [Histonet] Slide labels falling off- Slidemate AS Stefanie, When you say "labels" are you talking about paper labels or the print on the slides from the Slidemate?? I will assume that you mean the print, and yes, I have had the problem in the past where the print came off during staining and/or you could literally wipe the print off with a kimwipe or your finger. In our case, it was the slides - even though it was the same slide we had been using for years. This was happening with a certain lot number. Is this happening with just one lot number? Maybe you can try and get a different lot number or try a totally different slide (different manufacturer) to narrow down the issue. Good luck! Laurie -----Original Message----- From: Harris, Stefanie via Histonet To: histonet Sent: Wed, Mar 28, 2018 8:51 am Subject: [Histonet] Slide labels falling off- Slidemate AS Hello all, I am hoping someone out there can help. We have been having issues with some of the labels coming off during staining and after. It seems to be random so I can't tell if there is a problem with the slides or the Slidemate AS. There are days that it will be half the slides but other days where it will be none. Sometimes the labels peel off and float away during staining and other times labels rub off when handling but only after staining. We are using Globe Scientific Slides, and have been for over 2 years with no issues until December. I have changed out the ribbon on the slidemate and the problem is still sporadic. Oven time drying time does not seem to be a factor. We have not made any reagent changes for the staining process to explain the issue either. Has anyone else seen anything like this? Any slide suggestions to try out? Stefanie Harris Research Assistant, Histology Research Animal Diagnostic Services | Charles River 261 Ballardvale St., Wilmington, MA 01887 P: 781.222.6592 | F: 978.658.7698 stefanie.harris at crl.com | www.criver.com> LinkedIn> | Twitter> | Facebook> | Eureka> Confidentiality Notice: The information contained in this email and any attachments are confidential and may contain privileged and/or proprietary information. This email is intended for the use of the addressee only. If you are not the intended recipient, you are strictly prohibited from copying, distributing or using this email or the information contained in it. If you received this email in error, please notify the sender immediately by email or telephone, and destroy any copies, electronic, paper or otherwise. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtoole at dcol.net Wed Mar 28 11:18:32 2018 From: mtoole at dcol.net (Mike Toole) Date: Wed, 28 Mar 2018 11:18:32 -0500 Subject: [Histonet] Slide labels falling off- Slidemate AS In-Reply-To: <1626d57d898-17a0-68b8@webjas-vaa167.srv.aolmail.net> References: <1626d57d898-17a0-68b8@webjas-vaa167.srv.aolmail.net> Message-ID: <31530E35E0BAB044B3B56B7FE5CF4EB35703D31EC2@mail> Stefanie, >> the labels peel off and float away during staining Sounds like a paper type label or more likely a xylene resistant type label. Either way, try applying the label after the slides are stained instead of before staining. Mike -----Original Message----- From: Laurie Colbert via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, March 28, 2018 11:02 AM To: Stefanie.Harris at crl.com; histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Slide labels falling off- Slidemate AS Stefanie, When you say "labels" are? you talking about paper labels or the print on the slides from the Slidemate?? I will assume that you mean the print, and yes, I have had the problem in the past where the print came off during staining and/or you could literally wipe the print off with a kimwipe or your finger.? In our case, it was the slides - even though it was the same slide we had been using for years.? This was happening with a certain lot number.? Is this happening with just one lot number?? Maybe you can try and get a different lot number or try a totally different slide (different manufacturer) to narrow down the issue. Good luck! Laurie -----Original Message----- From: Harris, Stefanie via Histonet To: histonet Sent: Wed, Mar 28, 2018 8:51 am Subject: [Histonet] Slide labels falling off- Slidemate AS Hello all, I am hoping someone out there can help. We have been having issues with some of the labels coming off during staining and after. It seems to be random so I can't tell if there is a problem with the slides or the Slidemate AS. There are days that it will be half the slides but other days where it will be none. Sometimes the labels peel off and float away during staining and other times labels rub off when handling but only after staining. We are using Globe Scientific Slides, and have been for over 2 years with no issues until December. I have changed out the ribbon on the slidemate and the problem is still sporadic. Oven time drying time does not seem to be a factor. We have not made any reagent changes for the staining process to explain the issue either. Has anyone else seen anything like this? Any slide suggestions to try out? Stefanie Harris Research Assistant, Histology Research Animal Diagnostic Services | Charles River 261 Ballardvale St., Wilmington, MA 01887 P: 781.222.6592 | F: 978.658.7698 stefanie.harris at crl.com | www.criver.com LinkedIn | Twitter | Facebook | Eureka Confidentiality Notice: The information contained in this email and any attachments are confidential and may contain privileged and/or proprietary information. This email is intended for the use of the addressee only. If you are not the intended recipient, you are strictly prohibited from copying, distributing or using this email or the information contained in it. If you received this email in error, please notify the sender immediately by email or telephone, and destroy any copies, electronic, paper or otherwise. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From john.frazier at roche.com Wed Mar 28 13:16:24 2018 From: john.frazier at roche.com (Frazier, John) Date: Wed, 28 Mar 2018 11:16:24 -0700 Subject: [Histonet] Slide labels falling off- Slidemate AS Message-ID: Stephanie I am a histology workflow consultant. I have seen this issue at several sites that use the Thermo PrintMate. It uses a thermal print head along with the print tape to put printed data onto the frosted edge of a slide. Xylene being strong solvent and can easily damaged this printing during the H&E staining process. First of all I want to applaud anybody that is labeling their slides at the point of cutting. Anytime that you label the slides after staining, that forces you to handle label a slide then relabel the slide with the paper label. This is were 67% of all miss labeling errors occur. If this issue continues without resolution, I would look at moving to a xylene resistant label and printer. Continuing to applied the label at the point of cutting (microtomy). These labels are known to stick rather well and are not damaged by xylene. The label printers are not expensive, About $750 for each label printer vs. $10,000+ each for the Thermo PrintMate. Please let me know if there's anything else I can do to help you Sent from my iPhone > On Mar 28, 2018, at 10:36 AM, Harris, Stefanie wrote: > > Hello all, > > I am hoping someone out there can help. We have been having issues with some of the labels coming off during staining and after. It seems to be random so I can't tell if there is a problem with the slides or the Slidemate AS. There are days that it will be half the slides but other days where it will be none. Sometimes the labels peel off and float away during staining and other times labels rub off when handling but only after staining. > > > We are using Globe Scientific Slides, and have been for over 2 years with no issues until December. > > I have changed out the ribbon on the slidemate and the problem is still sporadic. > > Oven time drying time does not seem to be a factor. > > We have not made any reagent changes for the staining process to explain the issue either. > > > Has anyone else seen anything like this? Any slide suggestions to try out? > > Stefanie Harris > Research Assistant, Histology > Research Animal Diagnostic Services | Charles River > 261 Ballardvale St., Wilmington, MA 01887 > P: 781.222.6592 | F: 978.658.7698 > stefanie.harris at crl.com | www.criver.com > LinkedIn | Twitter | Facebook | Eureka > > Confidentiality Notice: The information contained in this email and any attachments are confidential and may contain privileged and/or proprietary information. This email is intended for the use of the addressee only. If you are not the intended recipient, you are strictly prohibited from copying, distributing or using this email or the information contained in it. If you received this email in error, please notify the sender immediately by email or telephone, and destroy any copies, electronic, paper or otherwise. > From lmarie08 at uga.edu Wed Mar 28 13:24:29 2018 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Wed, 28 Mar 2018 18:24:29 +0000 Subject: [Histonet] microarray question Message-ID: Hello Histoworld, I was wondering if anyone out there has any experience with microarrays? Making the blocks and cutting them. We have been having a hard time with all the nuances of this process and were wondering if you could share any tips/tricks you have learned doing this process. Recently, I have been wondering if there is a special kind of embedding medium that may work better. We have tried with Paraplast and Paraplast Plus and it seems like regular is better in general. But even still, we have cracks that form, and sometimes the block pops off while cutting on the microtome and also we never seem to have every punch unroll when laying the ribbon out on the waterbath. Please help! Thanks, Lauren From srhthacker at hotmail.com Wed Mar 28 15:12:49 2018 From: srhthacker at hotmail.com (Stephanie Hoyle-Thacker) Date: Wed, 28 Mar 2018 20:12:49 +0000 Subject: [Histonet] Tao Brush In-Reply-To: References: Message-ID: Hi, Is anyone processing endometrial biopsies received on a Tao brush? If so what is your protocol? Do you process the specimens as a surgical specimen (filtered into a bag) or as a cell block? Thanks so much! Renee Thacker From jobposting2600 at gmail.com Wed Mar 28 16:14:22 2018 From: jobposting2600 at gmail.com (Office Manager) Date: Wed, 28 Mar 2018 14:14:22 -0700 Subject: [Histonet] Please Post - Looking for Primera Cassette Printer Message-ID: We are looking for Primera Cassette Printer or similar, used and regardless of condition. Please contact: Isaac Malagon imalagon at evgastro.com (480) 231-9464 From Ryan.Roy at va.gov Thu Mar 29 07:43:20 2018 From: Ryan.Roy at va.gov (Roy, Ryan) Date: Thu, 29 Mar 2018 12:43:20 +0000 Subject: [Histonet] Syringe Pump error Ventana?? Message-ID: Does anybody know what the Syringe Pump is on the Ventana Benchmark Special Stainer?? What does it refer to in layman's terms? The operators manual is really vague, unless I missed a page? Ryan Roy BS, HTL (ASCP)CM Laboratory: Histology Department Manchester VA Medical Center Manchester NH 603 -624-4366 extension 6640 ** Disclaimer: The content of this email does not reflect the views or opinions of the Veterans Administration*** From katherine at ka-recruiting.com Thu Mar 29 09:00:05 2018 From: katherine at ka-recruiting.com (Katherine Marano) Date: Thu, 29 Mar 2018 10:00:05 -0400 Subject: [Histonet] Histotech Needed in Southern California Message-ID: Hi Histonetters, Is anyone interested (or know of anyone interested) in a permanent position with a top hospital in Southern California? Let me know asap! Here are some details I have: Full-time Histotech at a 500 bed accredited teaching hospital in southeastern California. Department: Histology Schedule: Full Time, Days Job Objective: The position prepares and stains tissue sections from surgical and autopsy material for microscopic interpretation by the pathologist. Reports to: Histology Supervisor Qualifications: * Graduate of an accredited school of Histologic Technique or 2 yrs on the job training under Board certified Pathologist * ASCP CA state certification * 1 year working as a certified histology technician or 2 years completion of training under Board certified Pathologist Essential Responsibilities: - Demonstrates compliance with Code of Conduct and compliance policies, and takes action to resolve compliance questions or concerns and report suspected violations. - Processes surgical specimens in accordance with established procedures. - Processes all necessary paperwork, assuring appropriate reporting. - Embeds, sections, and stains tissue for microscopic interpretation by the pathologist. - Performs frozen sections and staining as needed. - Utilizes Laboratory Information Systems as appropriate. - Disinfects and maintains work areas and instrumentation as needed. - Performs preventative maintenance procedures. - Demonstrates proficiency on Laboratory equipment and instrumentation. - Complies with hospital and Laboratory safety policies and procedures. - Performs general duties in grossing area as needed. - Performs quality control and maintains records. - Assists in ordering, maintaining, and restocking of supplies. - Monitors block and slide storage and disposal. - Performs other duties as necessary. Offering competitive compensation and complete benefits! Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com