From melissa at alliedsearchpartners.com Fri Jun 1 09:01:41 2018 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Fri, 1 Jun 2018 14:01:41 +0000 Subject: [Histonet] Histology Manager job in Houston, TX Message-ID: Good Morning Histonet, I have a Histology Manager (Full Time/Permanent) opening in Houston, TX. If you are a manager or supervisor and are interested please reach out to me for details. Accepting candidates nationwide as this position offers relocation assistance. Thank you! Melissa Owens, CHP (ASA) President, Laboratory Staffing T: 888.388.7571 ext. 102 Direct Line: 407.413.9117 F: 888.388.7572 From greg.dobbin at gmail.com Fri Jun 1 09:34:15 2018 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Fri, 1 Jun 2018 11:34:15 -0300 Subject: [Histonet] Slide and block retention -Peds seperated? Message-ID: Hello all, I would like to find out what other clinical path labs are doing in Canada in particular but in the US as well with regard to retention of blocks and slides. Are other labs filing pediatric cases separately from adult cases? I am required to archive specimens from pediatric cases separately from adults because our retention schedule states that specimens for adults will be retained for *20 years* while specimens from pediatric cases will be retained for *20 years after the patient reaches the age of majority* (18 years old). So for example, a specimen on a newborn will be kept for 38 years while a specimen on a 17-year-old will be kept for 21 years. This is no small task to achieve. We must generate a list of pediatric cases 4 times a year, pull these cases out of the file, file them by birth year in another area separate from our regular archived material, maintain and update a separate hardcopy case list, etc. I am proposing to management that we keep all cases for 38 years so that we can eliminate this task of separating peds from adults. However, before my request will be entertained, I must be able to point to other path labs that are not separating their peds cases. Thank you for your time! Greg -- *Greg Dobbin?, MLT* Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 From Timothy.Morken at ucsf.edu Fri Jun 1 13:53:15 2018 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 1 Jun 2018 18:53:15 +0000 Subject: [Histonet] Warm up freezer-stored powder, or use right away? In-Reply-To: References: Message-ID: Here is the answer I got from Sigma-Aldrich Tech Support. " We would not recommend bringing the entire bottle up to room temperature to weigh. Repeated freeze/thaw cycles can degrade the product. If you are worried about ice crystals affecting weight, you can bring a small sample up to room temp before weighing, or weigh out directly from the cold bottle and reweigh after equilibration." Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, May 25, 2018 10:48 AM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Warm up freezer-stored powder, or use right away? Tim Morken (Pathology Site Manager, Parnassus - Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology - UC San Francisco Medical Center) asks: >>We have a debate going on for those freezer-stored powdered chemicals used for enzyme histochemistry. One side says warm up the container to room temperature before opening in order to prevent water condensation in the container/powder. The other side says to use right away and put back in the freezer to avoid long exposure at room temperature. I'd like to hear pros and cons of each and any references stating one practice or the other.<< Opening that freezer-cold bottle before it warms up is ruinous. What I was taught to do in a research histochemistry lab (yeeks, this is a little less than 50 years ago) is to weigh out the needed quantity of the chemical (say, 10 mg of ATP) into empty gelatin capsules, and put the filled capsules in the freezer. When you do the procedure, take a single capsule out of the freezer. You could make up big batches of these capsules on a slow day. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at dpspa.com Fri Jun 1 14:07:14 2018 From: criley at dpspa.com (Charles Riley) Date: Fri, 1 Jun 2018 15:07:14 -0400 Subject: [Histonet] RET IHC testing Message-ID: Does anyone perform this testing? If so who do you get your antibody from and what platform do you run it on. Trying to figure out where to buy the antibody from. Looking for thoughts on cost and ease of use from suppliers. I use the Leica Bond platforms -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From pablo.sanchez at usc.es Sun Jun 3 13:00:43 2018 From: pablo.sanchez at usc.es (SANCHEZ QUINTEIRO PABLO) Date: Sun, 3 Jun 2018 18:00:43 +0000 Subject: [Histonet] Bouin postfixation Message-ID: Dear histonetters, I have to do immunohistochemistry with an antibody that works better in Bouin fixed samples. But I am afraid that my samples -just unprocessed- are in formalin. Do you think that it could be help to transfer them to Bouin 24 hours and then 70 ethanol and embedding? Thanks in advance Pablo Sanchez-Quinteiro Lugo. Spain From Katie.Sousa at baystatehealth.org Mon Jun 4 13:46:19 2018 From: Katie.Sousa at baystatehealth.org (Sousa, Katie) Date: Mon, 4 Jun 2018 18:46:19 +0000 Subject: [Histonet] GI biopsies Message-ID: Taking a survey: how many levels do you all cut for GI biopsy specimens? Katie Sousa Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786 Fax: 413-322-4790 Katie.sousa at baystatehealth.org ---------------------------------------------------------------------- Please view our annual report at http://www.bhannualreport.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at 413-794-0000 and destroy all copies of this communication and any attachments. For further information regarding Baystate Health's privacy policy, please visit our Internet site at https://www.baystatehealth.org. From carl.hobbs at kcl.ac.uk Mon Jun 4 14:21:32 2018 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Mon, 4 Jun 2018 19:21:32 +0000 Subject: [Histonet] Bouin postfixation In-Reply-To: References: Message-ID: Hi Pablo What is the name, source and catalogue number of your antibody? ( maybe you do not need to fix in Bouin's fixing fluid....do you know, as a fact, that Bouin's fix is better?) Imho, once you have fixed in Formalin ( depending on the time in fixing fluid), you cannot successfully "refix" in another fixative. Also: "embedding": do you mean in OCT or Pwax? Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: 04 June 2018 18:00 To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 175, Issue 3 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C3fc139402a6e4a115ffa08d5ca3f90d2%7C8370cf1416f34c16b83c724071654356%7C0&sdata=lkgAB3JHrCmogfTpMGuXOjWVnYxZqGLoE7DKkcRxsD0%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Bouin postfixation (SANCHEZ QUINTEIRO PABLO) ---------------------------------------------------------------------- Message: 1 Date: Sun, 3 Jun 2018 18:00:43 +0000 From: SANCHEZ QUINTEIRO PABLO To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Bouin postfixation Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dear histonetters, I have to do immunohistochemistry with an antibody that works better in Bouin fixed samples. But I am afraid that my samples -just unprocessed- are in formalin. Do you think that it could be help to transfer them to Bouin 24 hours and then 70 ethanol and embedding? Thanks in advance Pablo Sanchez-Quinteiro Lugo. Spain ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C3fc139402a6e4a115ffa08d5ca3f90d2%7C8370cf1416f34c16b83c724071654356%7C0&sdata=lkgAB3JHrCmogfTpMGuXOjWVnYxZqGLoE7DKkcRxsD0%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 175, Issue 3 **************************************** From mills at 3scan.com Mon Jun 4 16:54:48 2018 From: mills at 3scan.com (Caroline Miller) Date: Mon, 4 Jun 2018 14:54:48 -0700 Subject: [Histonet] GI biopsies In-Reply-To: References: Message-ID: In London, at the Hammersmith, we used to do 3 on two slides, one H+E and the other spare. mills ? On Mon, Jun 4, 2018 at 11:57 AM Sousa, Katie via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Taking a survey: how many levels do you all cut for GI biopsy specimens? > > Katie Sousa > Supervisor Histology > Baystate Medical Center > 361 Whitney Ave., Holyoke, MA 01040 > Telephone: 413-322-4786 Fax: 413-322-4790 > Katie.sousa at baystatehealth.org > > > ---------------------------------------------------------------------- > Please view our annual report at http://www.bhannualreport.org > > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may > contain confidential and privileged information for the use of the > designated recipients named above. If you are not the intended recipient, > you are hereby notified that you have received this communication in error > and that any review, disclosure, dissemination, distribution or copying of > it or its contents is prohibited. If you have received this communication > in error, please reply to the sender immediately or by telephone at > 413-794-0000 and destroy all copies of this communication and any > attachments. For further information regarding Baystate Health's privacy > policy, please visit our Internet site at https://www.baystatehealth.org. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Biology www.3Scan.com 415 2187297 From pablo.sanchez at usc.es Mon Jun 4 18:11:16 2018 From: pablo.sanchez at usc.es (SANCHEZ QUINTEIRO PABLO) Date: Mon, 4 Jun 2018 23:11:16 +0000 Subject: [Histonet] Bouin postfixation In-Reply-To: References: , Message-ID: Thanks for your input and for your curiosity Carl, The antibody is raised against G protein subunit alpha i2. Made by Santa Cruz Biotechnologies SC-7276. I have employed it for several years and from my experience with my protocols it works much better in Bouin's than in formalin. The embedding is in paraffin. Pablo ________________________________ De: Hobbs, Carl via Histonet Enviado: lunes, 4 de junio de 2018 21:21:32 Para: histonet at lists.utsouthwestern.edu Asunto: Re: [Histonet] Bouin postfixation Hi Pablo What is the name, source and catalogue number of your antibody? ( maybe you do not need to fix in Bouin's fixing fluid....do you know, as a fact, that Bouin's fix is better?) Imho, once you have fixed in Formalin ( depending on the time in fixing fluid), you cannot successfully "refix" in another fixative. Also: "embedding": do you mean in OCT or Pwax? Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: 04 June 2018 18:00 To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 175, Issue 3 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C3fc139402a6e4a115ffa08d5ca3f90d2%7C8370cf1416f34c16b83c724071654356%7C0&sdata=lkgAB3JHrCmogfTpMGuXOjWVnYxZqGLoE7DKkcRxsD0%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Bouin postfixation (SANCHEZ QUINTEIRO PABLO) ---------------------------------------------------------------------- Message: 1 Date: Sun, 3 Jun 2018 18:00:43 +0000 From: SANCHEZ QUINTEIRO PABLO To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Bouin postfixation Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dear histonetters, I have to do immunohistochemistry with an antibody that works better in Bouin fixed samples. But I am afraid that my samples -just unprocessed- are in formalin. Do you think that it could be help to transfer them to Bouin 24 hours and then 70 ethanol and embedding? Thanks in advance Pablo Sanchez-Quinteiro Lugo. Spain ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7C3fc139402a6e4a115ffa08d5ca3f90d2%7C8370cf1416f34c16b83c724071654356%7C0&sdata=lkgAB3JHrCmogfTpMGuXOjWVnYxZqGLoE7DKkcRxsD0%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 175, Issue 3 **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From boznpl at aol.com Wed Jun 6 08:50:05 2018 From: boznpl at aol.com (Laurie Colbert) Date: Wed, 6 Jun 2018 09:50:05 -0400 Subject: [Histonet] V600E Message-ID: <163d55c97fa-c90-c8a@webjasstg-vab31.srv.aolmail.net> Can anyone tell me about the V600E antibody?? Our pathologist wants to add it to our menu of IHC stains, and I've never heard of it.? Sources? Thanks, Laurie Redmond From boznpl at aol.com Wed Jun 6 08:59:37 2018 From: boznpl at aol.com (Laurie Colbert) Date: Wed, 6 Jun 2018 09:59:37 -0400 Subject: [Histonet] Silver disposal Message-ID: <163d565529f-c8f-1b9ed@webjasstg-vab46.srv.aolmail.net> I'm wondering how others dispose of their silver waste from a GMS stain?? We have always collected and disposed of it separately from our other chemical waste.? We have a 55 gallon drum where we dispose of formalin, alcohol and xylene, and I was told by several people that we could just add the silver into the 55 gallon drum since it was such a small percentage of silver. We use 10 ml of a 5% silver solution and then dilute that to 50 ml, so there is a very small percentage of silver in the solution. Thanks, Laurie Redmond From criley at dpspa.com Wed Jun 6 09:15:41 2018 From: criley at dpspa.com (Charles Riley) Date: Wed, 6 Jun 2018 10:15:41 -0400 Subject: [Histonet] V600E In-Reply-To: <163d55c97fa-c90-c8a@webjasstg-vab31.srv.aolmail.net> References: <163d55c97fa-c90-c8a@webjasstg-vab31.srv.aolmail.net> Message-ID: Our paths just asked me to add this antibody also. Ventana-Roche is one source but we don't deal with them because of pricing issues. I believe we are purchasing from Sigma Aldrich instead. I put the info my pathologist provided in the email below The following is a message conveyed through the advertising activities of the journal. Whether in print or online, the CAP does not endorse products or services in paid advertising. SEE MORE WITH THE VENTANA MMR IHC PANEL The Class II IVD detects deficiency of any of the four mismatch repair (MMR) proteins as an aid in identifying probable Lynch syndrome in patients diagnosed with colorectal cancer (CRC). The VENTANA MMR IHC Panel includes the BRAF V600E (VE1) antibody, which helps you refine the number of patients who receive additional testing for Lynch syndrome by identifying sporadic CRC in the absence of MLH1 expression. The ready-to-use panel is optimized for use with the VENTANA OptiView DAB IHC Detection Kit on the fully automated VENTANA BenchMark ULTRA instrument. Deliver diagnostic confidence for colorectal cancer patients VENTANA MMR IHC Panel MLH1 (M1) antibody PMS2 (A16 - 4) antibody MSH2 (G219-1129) antibody MSH6 (SP93) antibody BRAF V600E (VE1) antibody Legal Statement Privacy Policy | Send us Feedback | Forward to Friend | Manage Email Settings This is an advertisement. VENTANA, OPTIVIEW, and BENCHMARK are trademarks of Roche. All other trademarks and product names are the property of their respective owners. ? 2018 Roche Diagnostics Corporation 9115 Hague Road ? PO Box 50414 ? Indianapolis, IN 46250 On Wed, Jun 6, 2018 at 9:50 AM, Laurie Colbert via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Can anyone tell me about the V600E antibody? Our pathologist wants to add > it to our menu of IHC stains, and I've never heard of it. Sources? > > Thanks, > Laurie Redmond > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From criley at dpspa.com Wed Jun 6 09:17:13 2018 From: criley at dpspa.com (Charles Riley) Date: Wed, 6 Jun 2018 10:17:13 -0400 Subject: [Histonet] Ventana Benchmak reagents disposal Message-ID: I have a ton of old expired unopened ventana reagents for the benchmarks and symphony machines. I work in Delaware and want to know if anyone knows what the proper disposal process would be. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From kaarrington at anthc.org Wed Jun 6 09:24:09 2018 From: kaarrington at anthc.org (Arrington, Karla A) Date: Wed, 6 Jun 2018 14:24:09 +0000 Subject: [Histonet] HT Position in Alaska Message-ID: <5d391657ea9f42d9b3b7a48191ed8fd1@anthc.org> Hello All, I hope everyone is enjoying the early part of summer. In beautiful Alaska, our hospital caters to the Alaskan Native population with their entire medical needs being met. Currently we have a Histology Technician position available in our pathology department. This position is M-F, no weekends and a rotating on-call schedule. This position comes with excellent benefits and a team work environment. This also offers relocation assistance for the correct candidate. Must be ASCP certified or eligible. For immediate consideration, email or FAX a current resume to kaarrington at anthc.org and it will be forwarded to our hiring manager. Also visit our website at anthc.org and select Careers to apply online. If you have questions regarding this position or how to submit an online application, please call ANTHC Human Resources at (907) 729-1301 or email careers at anthc.org. Karla Arrington, HT(ASCP) HIT(AHIMA) Pathology Supervisor Alaska Native Medical Center 4315 Diplomacy Drive Anchorage, AK 99508 Tele: 907-729-1810 Fax: 907-729-1226 From LNormington at uwhealth.org Wed Jun 6 09:43:17 2018 From: LNormington at uwhealth.org (Normington Lacy) Date: Wed, 6 Jun 2018 14:43:17 +0000 Subject: [Histonet] V600E In-Reply-To: <163d55c97fa-c90-c8a@webjasstg-vab31.srv.aolmail.net> References: <163d55c97fa-c90-c8a@webjasstg-vab31.srv.aolmail.net> Message-ID: I am thinking this might be BRAF, clone V600E. We get ours from Ventana Roche. Lacy Normington UW Health Madison, WI -----Original Message----- From: Laurie Colbert via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, June 06, 2018 8:50 AM To: histonet at lists.utsouthwestern.edu Cc: jtouchstone at pathmdlabs.com Subject: [Histonet] V600E WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Can anyone tell me about the V600E antibody? Our pathologist wants to add it to our menu of IHC stains, and I've never heard of it. Sources? Thanks, Laurie Redmond _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster at umn.edu Wed Jun 6 11:10:46 2018 From: cforster at umn.edu (Colleen Forster) Date: Wed, 6 Jun 2018 11:10:46 -0500 Subject: [Histonet] HT Position in Alaska In-Reply-To: <5d391657ea9f42d9b3b7a48191ed8fd1@anthc.org> References: <5d391657ea9f42d9b3b7a48191ed8fd1@anthc.org> Message-ID: This sounds wonderful......I wish I were in a position to swoop it up! Colleen Forster HT(ASCP)QIHC On Wed, Jun 6, 2018 at 9:24 AM, Arrington, Karla A via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello All, > > I hope everyone is enjoying the early part of summer. > > In beautiful Alaska, our hospital caters to the Alaskan Native population > with their entire medical needs being met. > Currently we have a Histology Technician position available in our > pathology department. This position is M-F, no weekends and a rotating > on-call schedule. > This position comes with excellent benefits and a team work environment. > This also offers relocation assistance for the correct candidate. Must be > ASCP certified or eligible. > > For immediate consideration, email or FAX a current resume to > kaarrington at anthc.org and it will be > forwarded to our hiring manager. Also visit our website at anthc.org and > select Careers to apply online. > > If you have questions regarding this position or how to submit an online > application, please call ANTHC Human Resources at (907) 729-1301 or email > careers at anthc.org. > > > Karla Arrington, HT(ASCP) HIT(AHIMA) > Pathology Supervisor > Alaska Native Medical Center > 4315 Diplomacy Drive > Anchorage, AK 99508 > Tele: 907-729-1810 > Fax: 907-729-1226 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at holml at umn.edu * From bakevictoria at gmail.com Wed Jun 6 12:20:41 2018 From: bakevictoria at gmail.com (Victoria Baker) Date: Wed, 6 Jun 2018 13:20:41 -0400 Subject: [Histonet] HT Position in Alaska In-Reply-To: References: <5d391657ea9f42d9b3b7a48191ed8fd1@anthc.org> Message-ID: Me as well. It's beautiful there! On Wed, Jun 6, 2018, 12:27 PM Colleen Forster via Histonet < histonet at lists.utsouthwestern.edu> wrote: > This sounds wonderful......I wish I were in a position to swoop it up! > > Colleen Forster HT(ASCP)QIHC > > On Wed, Jun 6, 2018 at 9:24 AM, Arrington, Karla A via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hello All, > > > > I hope everyone is enjoying the early part of summer. > > > > In beautiful Alaska, our hospital caters to the Alaskan Native population > > with their entire medical needs being met. > > Currently we have a Histology Technician position available in our > > pathology department. This position is M-F, no weekends and a rotating > > on-call schedule. > > This position comes with excellent benefits and a team work environment. > > This also offers relocation assistance for the correct candidate. Must be > > ASCP certified or eligible. > > > > For immediate consideration, email or FAX a current resume to > > kaarrington at anthc.org and it will be > > forwarded to our hiring manager. Also visit our website at anthc.org and > > select Careers to apply online. > > > > If you have questions regarding this position or how to submit an online > > application, please call ANTHC Human Resources at (907) 729-1301 or email > > careers at anthc.org. > > > > > > Karla Arrington, HT(ASCP) HIT(AHIMA) > > Pathology Supervisor > > Alaska Native Medical Center > > 4315 Diplomacy Drive > > Anchorage, AK 99508 > > Tele: 907-729-1810 > > Fax: 907-729-1226 > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Colleen Forster HT(ASCP)QIHC > BLS Histology and IHC Laboratory > B173 PWB 612-626-1930 > > *If submitting histology request please also forward to Lori Holm at > holml at umn.edu * > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sbaldwin at mhhcc.org Wed Jun 6 13:08:46 2018 From: sbaldwin at mhhcc.org (Baldwin, Kathy) Date: Wed, 6 Jun 2018 18:08:46 +0000 Subject: [Histonet] pH of formalin Message-ID: <244c8c186606435fbc7c30783bd74892@exch02.mhhcc.org> Hi Histonetters Is anyone out there monitoring their Ph of the formalin they use? If do how often? Thanks S Kathy Baldwin ASCP, SCT Pathology and Cytology Manager Ph 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tony.henwood at health.nsw.gov.au Wed Jun 6 20:52:49 2018 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Thu, 7 Jun 2018 01:52:49 +0000 Subject: [Histonet] pH of formalin In-Reply-To: <244c8c186606435fbc7c30783bd74892@exch02.mhhcc.org> References: <244c8c186606435fbc7c30783bd74892@exch02.mhhcc.org> Message-ID: <0237449DE79DBC45B686AB82CDCD16FFB795DC52@SVDCMBX-MEX008.nswhealth.net> We routinely use the following Hew-Shue (1991) has described a useful pH indicator for working neutral buffered formalin solutions. Bromocresol purple (CAS: 115-40-2) , when added to formalin solutions, serves as an indicator of pH as well as, from a safety aspect, labelling the solution as formalin making redundant the dangerous "smell" test. At an acidic pH (5.2) the indicator colour is yellow whereas at pH 6.8, the colour is purple. A saturated solution of the dye (about 0.2g/100mls) is prepared and 2 to 4 drops are added to 10 litres of neutral buffered formalin. Hew-Shue N (1991) "Bromcresol Purple as a Colored Marker and pH Indicator for Ten Percent Neutral Buffered Forrnalin" J Histotechnol 14(4):257-260. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Baldwin, Kathy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, 7 June 2018 4:09 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] pH of formalin Hi Histonetters Is anyone out there monitoring their Ph of the formalin they use? If do how often? Thanks S Kathy Baldwin ASCP, SCT Pathology and Cytology Manager Ph 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From boznpl at aol.com Wed Jun 6 21:03:58 2018 From: boznpl at aol.com (Laurie Colbert) Date: Wed, 6 Jun 2018 22:03:58 -0400 Subject: [Histonet] pH of formalin In-Reply-To: <244c8c186606435fbc7c30783bd74892@exch02.mhhcc.org> References: <244c8c186606435fbc7c30783bd74892@exch02.mhhcc.org> Message-ID: <163d7fc7b76-c8a-42ff@webjasstg-vab32.srv.aolmail.net> We monitor the concentration and pH of our recycled formalin weekly, but since it has been consistently within range I may start monitoring monthly. -----Original Message----- From: Baldwin, Kathy via Histonet To: 'histonet at lists.utsouthwestern.edu' Sent: Wed, Jun 6, 2018 11:25 am Subject: [Histonet] pH of formalin Hi Histonetters Is anyone out there monitoring their Ph of the formalin they use? If do how often? Thanks S Kathy Baldwin ASCP, SCT Pathology and Cytology Manager Ph 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From naira.margaryan at hsc.wvu.edu Thu Jun 7 08:04:32 2018 From: naira.margaryan at hsc.wvu.edu (Margaryan, Naira) Date: Thu, 7 Jun 2018 13:04:32 +0000 Subject: [Histonet] Coverslipper Message-ID: Happy Thursday, We would like to sell our slightly used but in excellent condition Dako Coverslipper which was purchased in 2010 $10K (purchase price was $ 25K). The instrument can handle up to 600 slides per hour making it one of the fastest on the market. In addition to the flexibility of the Coverslipper it is easy and straightforward to operate and cleaning and maintenance is simple to do. It is small enough to fit into fume cabinets, easy to move around and accepts a variety of commercial mounting media. Picture will send by request. Will take any offer, Naira From jeanine.ronkowski at yahoo.com Thu Jun 7 08:52:05 2018 From: jeanine.ronkowski at yahoo.com (Jeanine Ronkowski) Date: Thu, 7 Jun 2018 13:52:05 +0000 (UTC) Subject: [Histonet] IHC System from StatLab References: <2117531429.1365910.1528379525247.ref@mail.yahoo.com> Message-ID: <2117531429.1365910.1528379525247@mail.yahoo.com> I?ve heard from a number of people that Stat Lab plans to release a new instrument for immunostaining and I was wondering if someone, including the folks at StatLab, might be able to provide more information.Thx,Jeanine Sent from Yahoo Mail for iPhone From c.tague at Pathologyarts.com Thu Jun 7 11:14:16 2018 From: c.tague at Pathologyarts.com (Curt) Date: Thu, 7 Jun 2018 16:14:16 +0000 Subject: [Histonet] lieca bond stainers available Message-ID: <9C8F910F72893643B3C3793C3D67132B837D064A@PATHOLOGYSERVER.pathologyarts.local> I've got 2 stainers that have been shelved for a couple years, anyone interested in them? Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. From CDavis at che-east.org Thu Jun 7 11:52:28 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Thu, 7 Jun 2018 16:52:28 +0000 Subject: [Histonet] USA to Canada Message-ID: <95fe1552251745acb42ba6c51f6f6c29@che-east.org> Hello Histofolks, A friend asked if our HT/HTL are transferable to Canada I said no but that got me to wondering...what does a HT/HTL have to do to work in histology in Canada? Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From greg.dobbin at gmail.com Thu Jun 7 12:21:54 2018 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Thu, 7 Jun 2018 14:21:54 -0300 Subject: [Histonet] USA to Canada Message-ID: Pass The General MLT Certification exam, as our MLT exam includes Histology and Microbiology. :-) Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From relia1 at earthlink.net Fri Jun 8 09:47:22 2018 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 8 Jun 2018 10:47:22 -0400 Subject: [Histonet] Summer is here!!! Here are some RELIA EXCLUSIVES!!! Get em' while they're HOT!!! Message-ID: <008c01d3ff37$9be33800$d3a9a800$@earthlink.net> Hi Histonetters, Summer is Here!!!! What tells you that Summer is here? That first bite of watermelon OR sip of ice cold lemonade on a hot day? That first dip in the shimmering turquoise waters of a pool, lake or ocean? That Cookout, Baseball Game, State Fair, Fresh Corn? PROM, GRADUATION SUMMER VACATION!! I Love it all, except maybe the heat and bathing suit shopping (UGH!!) Another way I can tell that Summer is here is that my phone also starts ringing off the hook. Clients are calling from all over the country. It happens every year right after Memorial Day. I Have A Bunch Of New Opportunities To Tell You About And New Ones Coming In EVERY DAY!! Histonetters, please take a look at my current openings. If something looks interesting let me know. If you know someone who might be interested please pass along the information. Remember if I place someone you refer to me you will earn a referral fee. If the opportunity for you is someplace else drop me an email to remind me where you want to be and what you want to do. I want YOU to be the person that pops in MY head when that client calls!!! Here are my Hottest!! Open Histology Opportunities: (**The exclusives are STARRED**) **PA Mohs Tech ? run your own lab!!** **TX Histology Tech** **OH Histology Supervisor** **OH Lead Histology Tech** **CA Lead Histology Tech** NC Histology Tech FL Histology Tech ME Lead Histology Tech All of my clients offer excellent compensation, benefits and some offer relocation assistance and or sign on bonuses. All of these jobs are full time & permanent & some are RELIA Exclusives!!! If you or anyone you know is interested in hearing more about any of these opportunities please contact me. Remember if I place someone you refer to me you will earn a referral bonus. This is all the info I can share publicly because I don't want my competitors to poach these positions from me or YOU! So please call or email me for more details!! If you want to chat ASAP call or text me at 407-353-5070 and if you want to set up a time to chat call the office at 866-607-3542 or email me at relia1 at earthlink.net with a time and the best number to call you!!!! I can?t wait to chat with you!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ? Pam Barker President RELIA Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From jriggleman at globusmedical.com Fri Jun 8 13:04:43 2018 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Fri, 8 Jun 2018 18:04:43 +0000 Subject: [Histonet] Goldner's Trichrome Thick Sections Message-ID: Hello, Looking for a protocol for staining thick plastic methyl methacrylate sections (100-150 um). Goldner?s or any trichrome stain would work. Thank You! Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From gu.lang at gmx.at Sun Jun 10 09:32:12 2018 From: gu.lang at gmx.at (Gudrun Lang) Date: Sun, 10 Jun 2018 16:32:12 +0200 Subject: [Histonet] V600E In-Reply-To: References: <163d55c97fa-c90-c8a@webjasstg-vab31.srv.aolmail.net> Message-ID: <000001d400c7$d3a4ecc0$7aeec640$@gmx.at> This is an mutationspecific antibody against BRAF. And the mutation detected is called p.V600E. The exchange of an nucleotid in the DNA (codon 600) leads to an tranformed and always activated BRAF-protein, that is sensitive to an thyrosinkinase-inhibitor as an eg. Melanoma therapy. As far as I remember it was sold by Novus Biological before Roche-Ventana had it. The clone is VE1. Gudrun -----Urspr?ngliche Nachricht----- Von: Normington Lacy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 6. Juni 2018 16:43 An: Laurie Colbert; 'histonet at lists.utsouthwestern.edu' Cc: jtouchstone at pathmdlabs.com Betreff: Re: [Histonet] V600E I am thinking this might be BRAF, clone V600E. We get ours from Ventana Roche. Lacy Normington UW Health Madison, WI -----Original Message----- From: Laurie Colbert via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, June 06, 2018 8:50 AM To: histonet at lists.utsouthwestern.edu Cc: jtouchstone at pathmdlabs.com Subject: [Histonet] V600E WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Can anyone tell me about the V600E antibody? Our pathologist wants to add it to our menu of IHC stains, and I've never heard of it. Sources? Thanks, Laurie Redmond _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang at gmx.at Sun Jun 10 09:37:58 2018 From: gu.lang at gmx.at (Gudrun Lang) Date: Sun, 10 Jun 2018 16:37:58 +0200 Subject: [Histonet] Bouin postfixation In-Reply-To: References: Message-ID: <000101d400c8$a0ee7bb0$e2cb7310$@gmx.at> The treatment with bouin afterwards doesn't make the formaldehyde-fixation undone. This would be rather a kind of antigen-retrieval and results at least in a mixture of both fixations. I would stay with the familiar formaldehyde-fixation without experiments and try to get the best out of it with the best fitting antigen-retrieval method. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: SANCHEZ QUINTEIRO PABLO via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Sonntag, 3. Juni 2018 20:01 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Bouin postfixation Dear histonetters, I have to do immunohistochemistry with an antibody that works better in Bouin fixed samples. But I am afraid that my samples -just unprocessed- are in formalin. Do you think that it could be help to transfer them to Bouin 24 hours and then 70 ethanol and embedding? Thanks in advance Pablo Sanchez-Quinteiro Lugo. Spain _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang at gmx.at Sun Jun 10 09:41:46 2018 From: gu.lang at gmx.at (Gudrun Lang) Date: Sun, 10 Jun 2018 16:41:46 +0200 Subject: [Histonet] RET IHC testing In-Reply-To: References: Message-ID: <000201d400c9$28833160$79899420$@gmx.at> I bought it from Cellsignalling (monoclonal rabbit), but just started with the procedure on lung tissue with no experience until now. I took ileum as positiv control, with stained Peyer-plaques as result. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Freitag, 1. Juni 2018 21:07 An: Histo List Betreff: [Histonet] RET IHC testing Does anyone perform this testing? If so who do you get your antibody from and what platform do you run it on. Trying to figure out where to buy the antibody from. Looking for thoughts on cost and ease of use from suppliers. I use the Leica Bond platforms -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang at gmx.at Sun Jun 10 10:04:29 2018 From: gu.lang at gmx.at (Gudrun Lang) Date: Sun, 10 Jun 2018 17:04:29 +0200 Subject: [Histonet] inhouse procedures in histotechnics Message-ID: <000001d400cc$54fbbf20$fef33d60$@gmx.at> Hi! What would you say is an inhouse-procedure in a typical clinical histo-lab. The question is regarded to accredidation in quality management. I'm asked to make a list of all inhouse-procedures. Special staining protocols? Immunhisto on stainer? I think histolabs are hardly comparable to typical medical/chemical labs with mainly commercial kits on instruments. Changing some duration of a staining incubation to get a better result - is this inhouse-made then?? What is the golden standard of all our stainings to refer to? Giemsa 1901? Masson 1929? Klebs (Paraffin embedding) 1871? Wissowzky,Busch (H&E) 1876? Mayer (hemalaun) 1891? ... and so on; you know what I mean? I think this is similar to a restaurant-kitchen, where each recipe is usually inhouse-made, although everyone is cooking "Pasta". Especially if you do the stainings by hand. (grumbling....) I know QM makes the lab-world better, but sometimes it is too much for me. ; ) Would be happy to get some input. thanks in advance Gudrun Lang Austria From michael.gudo at morphisto.de Mon Jun 11 04:09:47 2018 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Mon, 11 Jun 2018 11:09:47 +0200 Subject: [Histonet] Goldner's Trichrome Thick Sections In-Reply-To: References: Message-ID: Dear Jessica, what kind of MMA have you used? If it is Technovit 9100 we have an established procedure here in our lab. How many slides do you have? We can make you an offer to stain them here in our lab for you. There are several trichrome or polychrome stains possible. Best regards Michael > Am 08.06.2018 um 20:04 schrieb Jessica Riggleman via Histonet : > > Hello, > Looking for a protocol for staining thick plastic methyl methacrylate sections (100-150 um). Goldner?s or any trichrome stain would work. > > Thank You! > Jessica > > > > _____________________________________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > > Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From LRaff at uropartners.com Mon Jun 11 08:34:41 2018 From: LRaff at uropartners.com (Lester Raff MD) Date: Mon, 11 Jun 2018 13:34:41 +0000 Subject: [Histonet] Lab related blog post--Electronic Health Records Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF11ADB2F3@COLOEXCH01.uropartners.local> Hope everyone had a good weekend. http://www.chicagonow.com/downsize-maybe/2018/06/your-doctor-hates-the-electronic-health-record-i-love-it/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From margaret.debell at gmail.com Mon Jun 11 10:25:58 2018 From: margaret.debell at gmail.com (Maggie DeBell) Date: Mon, 11 Jun 2018 11:25:58 -0400 Subject: [Histonet] Microtome Knife Holder Message-ID: Hello, I am an undergraduate student looking for a universal knife holder for the Microm HM 355 S-2 microtome I am using. Does anybody have one that they can't use anymore, or know where I could find one inexpensively? Thank you, Maggie DeBell From cfields at mlkch.org Mon Jun 11 11:00:37 2018 From: cfields at mlkch.org (Carol G Fields) Date: Mon, 11 Jun 2018 16:00:37 +0000 Subject: [Histonet] Microtome Knife Holder In-Reply-To: References: Message-ID: <13a51d1be9b84539bf68d9988bffc9a5@mlk01exc02.mlkch.org> Call Southeast Pathology Inc. Southeast Pathology Instrument Service Address: 1719 Signal Point Rd, Charleston, SC 29412 Phone: (855) 731-7999 They are the best... Carole Fields, HT, ASCP -----Original Message----- From: Maggie DeBell via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Monday, June 11, 2018 8:26 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Microtome Knife Holder Hello, I am an undergraduate student looking for a universal knife holder for the Microm HM 355 S-2 microtome I am using. Does anybody have one that they can't use anymore, or know where I could find one inexpensively? Thank you, Maggie DeBell _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email message and any files transmitted are sent with confidentiality in mind and contain privileged or copyright information. You must not present this message to another party without gaining permission from the sender. If you are not the intended recipient you must not copy, distribute or use this email or the information contained in it for any purpose other than to notify Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. Any views expressed in this message are those of the sender, except where the sender specifically states them to be the views of Martin Luther King, Jr. - Los Angeles (MLK - LA) Healthcare Corporation and the Martin Luther King, Jr. Community Hospital. If you have received this message in error, please notify the sender immediately, and delete this email from your system. We do not guarantee that this material is free from viruses or any other defects although due care has been taken to minimize the risk. From criley at dpspa.com Tue Jun 12 07:00:17 2018 From: criley at dpspa.com (Charles Riley) Date: Tue, 12 Jun 2018 08:00:17 -0400 Subject: [Histonet] Blade guards Message-ID: Does anyone know where to purchase blade guards for cryotomes? I am looking for one that can be secured in place versus a free floating piece of plastic that could get bumped off -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From ASelf at tidelandshealth.org Tue Jun 12 08:18:02 2018 From: ASelf at tidelandshealth.org (Amy Self) Date: Tue, 12 Jun 2018 09:18:02 -0400 Subject: [Histonet] Quality Measure for Pathology Message-ID: Good Morning, I am looking for ideas/suggestions for QM Histology/Pathology. My QM director wants me to "measure" something that I can place on the lab dashboard. Thanks in advance for your help.. Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From LNormington at uwhealth.org Tue Jun 12 08:33:44 2018 From: LNormington at uwhealth.org (Normington Lacy) Date: Tue, 12 Jun 2018 13:33:44 +0000 Subject: [Histonet] Quality Measure for Pathology In-Reply-To: References: Message-ID: Some ideas: Proficiency Testing Corrected Results (Addendums/Amendment) volume Turnaround Time Monitoring Frozen Section TAT (critical call) Rejected Tests (empty bottles, etc) -----Original Message----- From: Amy Self via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 12, 2018 8:18 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Quality Measure for Pathology WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Good Morning, I am looking for ideas/suggestions for QM Histology/Pathology. My QM director wants me to "measure" something that I can place on the lab dashboard. Thanks in advance for your help.. Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek at digestivespecialists.com Tue Jun 12 11:54:54 2018 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Tue, 12 Jun 2018 12:54:54 -0400 Subject: [Histonet] Quality Measure for Pathology In-Reply-To: References: Message-ID: <5A2BD13465E061429D6455C8D6B40E392A83242CD8@IBMB7Exchange.digestivespecialists.com> Also a discrepancy between the number of pieces that are dictated at gross as being in a cassette compared to what is found in the cassette at embedding. You can record the number of blocks that need re-embedded and the number of slide recuts needed a month. Linda Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Normington Lacy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 12, 2018 9:34 AM To: Amy Self; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Quality Measure for Pathology Some ideas: Proficiency Testing Corrected Results (Addendums/Amendment) volume Turnaround Time Monitoring Frozen Section TAT (critical call) Rejected Tests (empty bottles, etc) -----Original Message----- From: Amy Self via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 12, 2018 8:18 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Quality Measure for Pathology WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Good Morning, I am looking for ideas/suggestions for QM Histology/Pathology. My QM director wants me to "measure" something that I can place on the lab dashboard. Thanks in advance for your help.. Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Tue Jun 12 13:21:11 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 12 Jun 2018 18:21:11 +0000 Subject: [Histonet] QM Dashboard Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF5DFCC@HRHEX02-HOS.holyredeemer.local> We use: 1. Report TAT 2. Discrepant pathology reports a. Internal vs External report correlation b. Frozen section vs Final diagnosis correlation c. Amended reports I liked the idea of piece count discrepancy or even poorly processed blocks. I never recommend using "recuts" as a QM because that is more often a product of the pathologists' preference or desire to see more tissue and does not reflect the quality of the sections (unless it is an incomplete facing issue). This can quickly become punitive for your department because this information is not presented to people who understand pathology. My 2 cents. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From lblazek at digestivespecialists.com Tue Jun 12 13:34:20 2018 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Tue, 12 Jun 2018 14:34:20 -0400 Subject: [Histonet] QM Dashboard In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8ECF5DFCC@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8ECF5DFCC@HRHEX02-HOS.holyredeemer.local> Message-ID: <5A2BD13465E061429D6455C8D6B40E392A83242D55@IBMB7Exchange.digestivespecialists.com> Terri, I completely agree with you on the recut issue but I separate the recuts by Quality Issue and just a deeper section. If a section is folded, has chatter or some other quality issue then I think it needs addressed as a Quality Management issue. Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Terri Braud via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 12, 2018 2:21 PM To: 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] QM Dashboard We use: 1. Report TAT 2. Discrepant pathology reports a. Internal vs External report correlation b. Frozen section vs Final diagnosis correlation c. Amended reports I liked the idea of piece count discrepancy or even poorly processed blocks. I never recommend using "recuts" as a QM because that is more often a product of the pathologists' preference or desire to see more tissue and does not reflect the quality of the sections (unless it is an incomplete facing issue). This can quickly become punitive for your department because this information is not presented to people who understand pathology. My 2 cents. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jeanine.ronkowski at yahoo.com Tue Jun 12 13:39:41 2018 From: jeanine.ronkowski at yahoo.com (Jeanine Ronkowski) Date: Tue, 12 Jun 2018 18:39:41 +0000 (UTC) Subject: [Histonet] IHC System from StatLab References: <1639649937.3894404.1528828781857.ref@mail.yahoo.com> Message-ID: <1639649937.3894404.1528828781857@mail.yahoo.com> All:For those of you who saw my posting last week and we?re wondering about the outcome, I?m sorry to say that I?ve received very little useful feedback. ?Two individuals from StatLab sent me separate,?cryptic messages indicating that they would be be happy to either meet with me or?provide a demo of this unnamed instrument, but neither have provided me with a brochure or any other documentation, as I requested in my response. I?ve never done this before, so I would like to ask this group ? Is this behavior normal? ?I?m gathering information on available instruments, and since I assume these folks would like to sell their new system, so why is it that no one will provide me with some basic information, like slide capacity, capabilities, reagent costs,?etc? Any and all feedback is appreciated. Jeanine Sent from Yahoo Mail for iPhone From rsrichmond at gmail.com Tue Jun 12 21:01:43 2018 From: rsrichmond at gmail.com (Bob Richmond) Date: Tue, 12 Jun 2018 22:01:43 -0400 Subject: [Histonet] Quality Measure for Pathology Message-ID: Amy Self, Histology Lab Senior Tech at Tidelands Georgetown Memorial Hospital in Georgetown SC asks: >>I am looking for ideas/suggestions for QM Histology/Pathology. My QM director wants me to "measure" something that I can place on the lab dashboard.<< Look at the August 2017 number of CAP Today for a really provocative article about the use of whole slide imaging (WSI) in quality assurance of slides. Bob Richmond Samurai Pathologist Maryville TN From gu.lang at gmx.at Wed Jun 13 03:18:33 2018 From: gu.lang at gmx.at (Gudrun Lang) Date: Wed, 13 Jun 2018 10:18:33 +0200 Subject: [Histonet] Quality Measure for Pathology In-Reply-To: <5A2BD13465E061429D6455C8D6B40E392A83242CD8@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E392A83242CD8@IBMB7Exchange.digestivespecialists.com> Message-ID: <000801d402ef$1fb4aa00$5f1dfe00$@gmx.at> Linda, How do you handle this key figure of discrepancies? Have you set a cut-off (per person, per day) for any consequencies? What are the consequencies, besides repeated training? thanks Gudrun -----Urspr?ngliche Nachricht----- Von: Blazek, Linda via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Dienstag, 12. Juni 2018 18:55 An: Normington Lacy; Amy Self Cc: histonet at lists.utsouthwestern.edu Betreff: Re: [Histonet] Quality Measure for Pathology Also a discrepancy between the number of pieces that are dictated at gross as being in a cassette compared to what is found in the cassette at embedding. You can record the number of blocks that need re-embedded and the number of slide recuts needed a month. Linda Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Normington Lacy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 12, 2018 9:34 AM To: Amy Self; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Quality Measure for Pathology Some ideas: Proficiency Testing Corrected Results (Addendums/Amendment) volume Turnaround Time Monitoring Frozen Section TAT (critical call) Rejected Tests (empty bottles, etc) -----Original Message----- From: Amy Self via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 12, 2018 8:18 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Quality Measure for Pathology WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Good Morning, I am looking for ideas/suggestions for QM Histology/Pathology. My QM director wants me to "measure" something that I can place on the lab dashboard. Thanks in advance for your help.. Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garreyf at gmail.com Wed Jun 13 04:35:57 2018 From: garreyf at gmail.com (Garrey Faller) Date: Wed, 13 Jun 2018 05:35:57 -0400 Subject: [Histonet] Quality Measure for Pathology In-Reply-To: References: Message-ID: <8E67634D-31D5-4E82-ABEB-62B3420D0089@gmail.com> Good ideas. I would get the pathologist(s) involved. Having just one monitor in my opinion would not fly in my book. Sent from my iPhone > On Jun 12, 2018, at 9:33 AM, Normington Lacy via Histonet wrote: > > Some ideas: > > Proficiency Testing > Corrected Results (Addendums/Amendment) volume > Turnaround Time Monitoring > Frozen Section TAT (critical call) > Rejected Tests (empty bottles, etc) > > > -----Original Message----- > From: Amy Self via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, June 12, 2018 8:18 AM > To: 'histonet at lists.utsouthwestern.edu' > Subject: [Histonet] Quality Measure for Pathology > > WARNING: This email appears to have originated outside of the UW Health email system. > DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. > > > > > Good Morning, > > I am looking for ideas/suggestions for QM Histology/Pathology. My QM director wants me to "measure" something that I can place on the lab dashboard. > > Thanks in advance for your help.. > > Amy Self > Histology Lab Senior Tech > Lab > Tidelands Georgetown Memorial Hospital > 606 Black River Road > Georgetown, SC 29440 > 843-520-8711 > ASelf at tidelandshealth.org > Our mission: We help people live better lives through better health. > NOTE: > The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek at digestivespecialists.com Wed Jun 13 07:37:34 2018 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Wed, 13 Jun 2018 08:37:34 -0400 Subject: [Histonet] Quality Measure for Pathology In-Reply-To: <000801d402ef$1fb4aa00$5f1dfe00$@gmx.at> References: <5A2BD13465E061429D6455C8D6B40E392A83242CD8@IBMB7Exchange.digestivespecialists.com> <000801d402ef$1fb4aa00$5f1dfe00$@gmx.at> Message-ID: <5A2BD13465E061429D6455C8D6B40E392A83242E56@IBMB7Exchange.digestivespecialists.com> Gudrun, Our lab is 95% biopsy specimens. Therefor the number of pieces counted at gross is written on the side of the cassette so that at the time of embedding the tech knows what should be present. That helps in being sure there isn't a piece stuck to the lid or forceps, or popped out when opening the cassette. It's really more of a Quality Assurance measure than tracking a discrepancy. I do watch how frequent there is a discrepancy though and if there seems to be a pattern will initiate a review of processes for both the grossing person and the embedding person. What we see most is the gross will say one piece and there will actually be multiple tiny pieces or two pieces will be three at embedding. As all staff is aware that we are following this they tend to be much more precise in counting at grossing and embedding. Linda -----Original Message----- From: Gudrun Lang [mailto:gu.lang at gmx.at] Sent: Wednesday, June 13, 2018 4:19 AM To: Blazek, Linda Cc: histonet at lists.utsouthwestern.edu Subject: AW: [Histonet] Quality Measure for Pathology Linda, How do you handle this key figure of discrepancies? Have you set a cut-off (per person, per day) for any consequencies? What are the consequencies, besides repeated training? thanks Gudrun -----Urspr?ngliche Nachricht----- Von: Blazek, Linda via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Dienstag, 12. Juni 2018 18:55 An: Normington Lacy; Amy Self Cc: histonet at lists.utsouthwestern.edu Betreff: Re: [Histonet] Quality Measure for Pathology Also a discrepancy between the number of pieces that are dictated at gross as being in a cassette compared to what is found in the cassette at embedding. You can record the number of blocks that need re-embedded and the number of slide recuts needed a month. Linda Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Normington Lacy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 12, 2018 9:34 AM To: Amy Self; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Quality Measure for Pathology Some ideas: Proficiency Testing Corrected Results (Addendums/Amendment) volume Turnaround Time Monitoring Frozen Section TAT (critical call) Rejected Tests (empty bottles, etc) -----Original Message----- From: Amy Self via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 12, 2018 8:18 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Quality Measure for Pathology WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Good Morning, I am looking for ideas/suggestions for QM Histology/Pathology. My QM director wants me to "measure" something that I can place on the lab dashboard. Thanks in advance for your help.. Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aeck at dh.org Wed Jun 13 08:04:17 2018 From: aeck at dh.org (Eck, Allison) Date: Wed, 13 Jun 2018 13:04:17 +0000 Subject: [Histonet] old cryostat Message-ID: <4ED8C96A8F20FC4F883A92E2A0A0D64AA708B5D6@DH-MAIL01.dhorg.org> Good morning, Thank you to everyone who responded to my frozen section question. The information I received was very useful. With that being said, I have an old cryostat, a Jung 1800. It no longer works as the refrigeration system has died but does anyone know of a company that would purchase (or even take) an old broken piece of equipment. It could potentially be scrapped for parts. I'm not sure how else to dispose of a cryostat. Thanks Allison Allison Eck, HTL(ASCP)cm,QLS, AHI(AMT),CEAS1 Lead Tech Histology Doylestown Hospital 595 W State St Doylestown, PA 18901 215-345-2264 aeck at dh.org From mkent at dermpathlab.com Wed Jun 13 09:07:07 2018 From: mkent at dermpathlab.com (Michael Kent) Date: Wed, 13 Jun 2018 14:07:07 +0000 Subject: [Histonet] Quality Measure for Pathology In-Reply-To: <8E67634D-31D5-4E82-ABEB-62B3420D0089@gmail.com> References: <8E67634D-31D5-4E82-ABEB-62B3420D0089@gmail.com> Message-ID: Good Morning: Repeated work: Slides that don't pass QC or the pathologists muster and require repeated embedding or cutting. E.G. because they are not cut deep enough, are missing the important features, needed to be soaked, inappropriate reactivity (IHC). This is different than levels, but things that can be caught at the bench. Mike -----Original Message----- From: Garrey Faller via Histonet Sent: Wednesday, June 13, 2018 5:36 AM To: Normington Lacy Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Quality Measure for Pathology Good ideas. I would get the pathologist(s) involved. Having just one monitor in my opinion would not fly in my book. Sent from my iPhone > On Jun 12, 2018, at 9:33 AM, Normington Lacy via Histonet wrote: > > Some ideas: > > Proficiency Testing > Corrected Results (Addendums/Amendment) volume Turnaround Time > Monitoring Frozen Section TAT (critical call) Rejected Tests (empty > bottles, etc) > > > -----Original Message----- > From: Amy Self via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Tuesday, June 12, 2018 8:18 AM > To: 'histonet at lists.utsouthwestern.edu' > > Subject: [Histonet] Quality Measure for Pathology > > WARNING: This email appears to have originated outside of the UW Health email system. > DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. > > > > > Good Morning, > > I am looking for ideas/suggestions for QM Histology/Pathology. My QM director wants me to "measure" something that I can place on the lab dashboard. > > Thanks in advance for your help.. > > Amy Self > Histology Lab Senior Tech > Lab > Tidelands Georgetown Memorial Hospital > 606 Black River Road > Georgetown, SC 29440 > 843-520-8711 > ASelf at tidelandshealth.org > Our mission: We help people live better lives through better health. > NOTE: > The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Dachel at va.gov Wed Jun 13 10:52:59 2018 From: Susan.Dachel at va.gov (Dachel, Susan K.) Date: Wed, 13 Jun 2018 15:52:59 +0000 Subject: [Histonet] ALK FISH Validation Protocol Message-ID: Does anybody have or have access to a validation protocol for FISH? Specifically, ALK and ROS testing in NSCLS cancer patients? How many samples are sufficient for validation? Where can one their hands on cases that are positive for ALK or ROS break apart (without buying tissue)? Thanks! Sue Dachel BA, HTL(ASCP)CM Histology Supervisor Minneapolis VA Health Care System From gu.lang at gmx.at Wed Jun 13 10:54:33 2018 From: gu.lang at gmx.at (Gudrun Lang) Date: Wed, 13 Jun 2018 17:54:33 +0200 Subject: [Histonet] Quality Measure for Pathology In-Reply-To: <5A2BD13465E061429D6455C8D6B40E392A83242E56@IBMB7Exchange.digestivespecialists.com> References: <5A2BD13465E061429D6455C8D6B40E392A83242CD8@IBMB7Exchange.digestivespecialists.com> <000801d402ef$1fb4aa00$5f1dfe00$@gmx.at> <5A2BD13465E061429D6455C8D6B40E392A83242E56@IBMB7Exchange.digestivespecialists.com> Message-ID: <000301d4032e$d40abf00$7c203d00$@gmx.at> Linda, that's exactly what we do here. I wondered, if you fixed a cut-off. Because in quality-audits they often asked the "what do you if.."-questions. for example: "There is an increase in the number of discrepancies in this special month. The rate is over your defined aim. What did you do to fix the problem? Do you keep records about the process of solving the problem?" I was just curious, if you go further than the counting of events. In daily work we often know the causes of mistakes, and very often we know, that a certain amount of mistakes can't be avoided, although everyone tries to give his/her best. I think QM should help to discover the special events or circumstances, that raise the number of mistakes above the "normal" level. I was curious, if you defined this "normal" level for your lab. Kind regards Gudrun -----Urspr?ngliche Nachricht----- Von: Blazek, Linda [mailto:lblazek at digestivespecialists.com] Gesendet: Mittwoch, 13. Juni 2018 14:38 An: Gudrun Lang Cc: histonet at lists.utsouthwestern.edu Betreff: RE: [Histonet] Quality Measure for Pathology Gudrun, Our lab is 95% biopsy specimens. Therefor the number of pieces counted at gross is written on the side of the cassette so that at the time of embedding the tech knows what should be present. That helps in being sure there isn't a piece stuck to the lid or forceps, or popped out when opening the cassette. It's really more of a Quality Assurance measure than tracking a discrepancy. I do watch how frequent there is a discrepancy though and if there seems to be a pattern will initiate a review of processes for both the grossing person and the embedding person. What we see most is the gross will say one piece and there will actually be multiple tiny pieces or two pieces will be three at embedding. As all staff is aware that we are following this they tend to be much more precise in counting at grossing and embedding. Linda -----Original Message----- From: Gudrun Lang [mailto:gu.lang at gmx.at] Sent: Wednesday, June 13, 2018 4:19 AM To: Blazek, Linda Cc: histonet at lists.utsouthwestern.edu Subject: AW: [Histonet] Quality Measure for Pathology Linda, How do you handle this key figure of discrepancies? Have you set a cut-off (per person, per day) for any consequencies? What are the consequencies, besides repeated training? thanks Gudrun -----Urspr?ngliche Nachricht----- Von: Blazek, Linda via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Dienstag, 12. Juni 2018 18:55 An: Normington Lacy; Amy Self Cc: histonet at lists.utsouthwestern.edu Betreff: Re: [Histonet] Quality Measure for Pathology Also a discrepancy between the number of pieces that are dictated at gross as being in a cassette compared to what is found in the cassette at embedding. You can record the number of blocks that need re-embedded and the number of slide recuts needed a month. Linda Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Normington Lacy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 12, 2018 9:34 AM To: Amy Self; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Quality Measure for Pathology Some ideas: Proficiency Testing Corrected Results (Addendums/Amendment) volume Turnaround Time Monitoring Frozen Section TAT (critical call) Rejected Tests (empty bottles, etc) -----Original Message----- From: Amy Self via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 12, 2018 8:18 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Quality Measure for Pathology WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Good Morning, I am looking for ideas/suggestions for QM Histology/Pathology. My QM director wants me to "measure" something that I can place on the lab dashboard. Thanks in advance for your help.. Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek at digestivespecialists.com Wed Jun 13 11:41:20 2018 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Wed, 13 Jun 2018 12:41:20 -0400 Subject: [Histonet] Quality Measure for Pathology In-Reply-To: <000301d4032e$d40abf00$7c203d00$@gmx.at> References: <5A2BD13465E061429D6455C8D6B40E392A83242CD8@IBMB7Exchange.digestivespecialists.com> <000801d402ef$1fb4aa00$5f1dfe00$@gmx.at> <5A2BD13465E061429D6455C8D6B40E392A83242E56@IBMB7Exchange.digestivespecialists.com> <000301d4032e$d40abf00$7c203d00$@gmx.at> Message-ID: <5A2BD13465E061429D6455C8D6B40E392A83242F58@IBMB7Exchange.digestivespecialists.com> Gudrun, Now you're making me think! I do have a cut-off. It's at 95%. I do keep records of the percentages of specimens that do not fall into the range. But as to the process of solving the problem it's just counseling. As we have only hit that cut-off once in the past three years I really haven't thought it out farther than that. Linda -----Original Message----- From: Gudrun Lang [mailto:gu.lang at gmx.at] Sent: Wednesday, June 13, 2018 11:55 AM To: Blazek, Linda Cc: histonet at lists.utsouthwestern.edu Subject: AW: [Histonet] Quality Measure for Pathology Linda, that's exactly what we do here. I wondered, if you fixed a cut-off. Because in quality-audits they often asked the "what do you if.."-questions. for example: "There is an increase in the number of discrepancies in this special month. The rate is over your defined aim. What did you do to fix the problem? Do you keep records about the process of solving the problem?" I was just curious, if you go further than the counting of events. In daily work we often know the causes of mistakes, and very often we know, that a certain amount of mistakes can't be avoided, although everyone tries to give his/her best. I think QM should help to discover the special events or circumstances, that raise the number of mistakes above the "normal" level. I was curious, if you defined this "normal" level for your lab. Kind regards Gudrun -----Urspr?ngliche Nachricht----- Von: Blazek, Linda [mailto:lblazek at digestivespecialists.com] Gesendet: Mittwoch, 13. Juni 2018 14:38 An: Gudrun Lang Cc: histonet at lists.utsouthwestern.edu Betreff: RE: [Histonet] Quality Measure for Pathology Gudrun, Our lab is 95% biopsy specimens. Therefor the number of pieces counted at gross is written on the side of the cassette so that at the time of embedding the tech knows what should be present. That helps in being sure there isn't a piece stuck to the lid or forceps, or popped out when opening the cassette. It's really more of a Quality Assurance measure than tracking a discrepancy. I do watch how frequent there is a discrepancy though and if there seems to be a pattern will initiate a review of processes for both the grossing person and the embedding person. What we see most is the gross will say one piece and there will actually be multiple tiny pieces or two pieces will be three at embedding. As all staff is aware that we are following this they tend to be much more precise in counting at grossing and embedding. Linda -----Original Message----- From: Gudrun Lang [mailto:gu.lang at gmx.at] Sent: Wednesday, June 13, 2018 4:19 AM To: Blazek, Linda Cc: histonet at lists.utsouthwestern.edu Subject: AW: [Histonet] Quality Measure for Pathology Linda, How do you handle this key figure of discrepancies? Have you set a cut-off (per person, per day) for any consequencies? What are the consequencies, besides repeated training? thanks Gudrun -----Urspr?ngliche Nachricht----- Von: Blazek, Linda via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Dienstag, 12. Juni 2018 18:55 An: Normington Lacy; Amy Self Cc: histonet at lists.utsouthwestern.edu Betreff: Re: [Histonet] Quality Measure for Pathology Also a discrepancy between the number of pieces that are dictated at gross as being in a cassette compared to what is found in the cassette at embedding. You can record the number of blocks that need re-embedded and the number of slide recuts needed a month. Linda Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com -----Original Message----- From: Normington Lacy via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 12, 2018 9:34 AM To: Amy Self; 'histonet at lists.utsouthwestern.edu' Subject: Re: [Histonet] Quality Measure for Pathology Some ideas: Proficiency Testing Corrected Results (Addendums/Amendment) volume Turnaround Time Monitoring Frozen Section TAT (critical call) Rejected Tests (empty bottles, etc) -----Original Message----- From: Amy Self via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, June 12, 2018 8:18 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Quality Measure for Pathology WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Good Morning, I am looking for ideas/suggestions for QM Histology/Pathology. My QM director wants me to "measure" something that I can place on the lab dashboard. Thanks in advance for your help.. Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jeanine.ronkowski at yahoo.com Thu Jun 14 05:29:32 2018 From: jeanine.ronkowski at yahoo.com (Jeanine Ronkowski) Date: Thu, 14 Jun 2018 10:29:32 +0000 (UTC) Subject: [Histonet] IHC System from StatLab References: <154037686.4832656.1528972172904.ref@mail.yahoo.com> Message-ID: <154037686.4832656.1528972172904@mail.yahoo.com> I?m pleased to report that I was finally contacted by a representative from StatLab that provided me with the information I was seeking. ?I realize that their system may not be fully released, but I didn?t think it would be that difficult for them to provide information. ?Turns out that this instrument is very similar to another manufacturer?s instrument that?s been on the market for a while.J Sent from Yahoo Mail for iPhone From Ryan.Roy at va.gov Thu Jun 14 07:18:32 2018 From: Ryan.Roy at va.gov (Roy, Ryan) Date: Thu, 14 Jun 2018 12:18:32 +0000 Subject: [Histonet] revised Cap regulation ANP .21395 ?? Message-ID: Hello Histonetters, What is the take home message of this revision? How are other labs complying with respect to Special staining? Do you keep records of individual case control verification or just individual block verification? Does anyone run NEG controls for Special staining? Is this regulation requiring Neg Controls for special stains? Any thoughts are appreciated. " **revised** 08/17/2016 ANP .21395 Special Stains/Studies For special stains, including histochemical stains, and studies using immunologic and ISH methodology, positive and negative controls are verified and recorded as acceptable prior to or concurrent with the reporting of patient results and records maintained. " Thanks you, Ryan Roy BS, HTL (ASCP)CM Laboratory: Histology Department Manchester VA Medical Center Manchester NH 603 -624-4366 extension 6640 ** Disclaimer: The content of this email does not reflect the views or opinions of the Veterans Administration*** From Linda.Margraf at cookchildrens.org Thu Jun 14 08:14:35 2018 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Thu, 14 Jun 2018 13:14:35 +0000 Subject: [Histonet] Tanner cryostat Message-ID: Here is a message I am posting for Bethany. Does anyone know anything about these cryostats imported from Germany? It's the Tanner TN50 cryostat. I'd like to hear from someone who has worked on one. We are looking to purchase a new cryostat. I love the QS12 but the Tanner is priced more reasonable. I can't find any reviews on this cryostat. Thanks! Bethany bmay at derminstitutemd.com From tbraud at holyredeemer.com Thu Jun 14 12:19:02 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 14 Jun 2018 17:19:02 +0000 Subject: [Histonet] CAP control revision Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF5E7F1@HRHEX02-HOS.holyredeemer.local> For all stains, we report the control verification on the patient report when reporting the stain results, and we run negative tissue controls for our organism stains, AFB and GMS Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 2. revised Cap regulation ANP .21395 ?? (Roy, Ryan) Message: 2 Date: Thu, 14 Jun 2018 12:18:32 +0000 From: "Roy, Ryan" Subject: [Histonet] revised Cap regulation ANP .21395 ?? Hello Histonetters, What is the take home message of this revision? How are other labs complying with respect to Special staining? Do you keep records of individual case control verification or just individual block verification? Does anyone run NEG controls for Special staining? Is this regulation requiring Neg Controls for special stains? Any thoughts are appreciated. " **revised** 08/17/2016 ANP .21395 Special Stains/Studies For special stains, including histochemical stains, and studies using immunologic and ISH methodology, positive and negative controls are verified and recorded as acceptable prior to or concurrent with the reporting of patient results and records maintained. " Thanks you, Ryan Roy BS, HTL (ASCP)CM Laboratory: Histology Department Manchester VA Medical Center Manchester NH 603 -624-4366 extension 6640 From CarenAnn.Echague at DignityHealth.org Thu Jun 14 14:23:23 2018 From: CarenAnn.Echague at DignityHealth.org (Echague, Caren Ann - MGH) Date: Thu, 14 Jun 2018 19:23:23 +0000 Subject: [Histonet] p16 dilution Message-ID: Hi Histonetters, Does any of you use p16 Cintec from Roche/Ventana? We wanted to dilute this antibody but wasn't sure how much to dilute it to. Figured we could save a lot by doing so. We have been staining our p16 with no current dilution. Any suggestions will help. If there are any other vendors that offer p16 other than Roche, that would be useful to know. Thanks! Cae Aguilar Histology Supervisor California From katherine at ka-recruiting.com Fri Jun 15 08:54:01 2018 From: katherine at ka-recruiting.com (Katherine Marano) Date: Fri, 15 Jun 2018 09:54:01 -0400 Subject: [Histonet] Histology Openings Message-ID: Hi Histonet members, Happy Friday! I wanted to check in and see you, or anyone you know, may be looking for a new permanent and full-time Histology position? I work with hospitals and labs across the country and I am currently looking to fill 10 excellent openings (see below). My clients offer competitive compensation, complete benefits, and many offer relocation assistance or sign on bonuses. Let me know if you are interested in hearing more about any of these!! *Histology* FL - Tampa - Histology Supervisor IN - East Chicago - Histotech (nights) NC - Charlotte - Histotech NM - Bayard - Histotech, Section Lead NY - White Plains - Histotech (1st & 2nd shifts open w/ rotating wknds) (IHC exp req'd) NY - White Plains- Grossing Tech OR - Salem - Histotech TX - Houston - Anatomic Pathology (Histology) Lab Manager VA - Richmond - Histotech (days) Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From bhartologist at gmail.com Fri Jun 15 17:09:00 2018 From: bhartologist at gmail.com (Bharti Parihar) Date: Fri, 15 Jun 2018 15:09:00 -0700 Subject: [Histonet] Staining Protocol for Chromosome Counting in Plants Message-ID: Hi ya'll. If there are any individuals out there that have done karyotyping, I need to try a protocol to get a chromosome count on a desert plant. We have germinated seeds with long roots, so sample size shouldn't be an issue. Tried a Orcein stain, but the results are quite lacking and it may not be the right stain for this plant. Gonna do a bit of modification with the fixative to specimen ratio to see if that helps, but it's possible this just isn't the right stain. After all, the stain ordered is for Arabidopsis, which is not our research plant. This is my first time doing plant histo, I'm used to human tissue, so I really don't know which stain to try. Anyone with any help/advice to offer, I would immensely appreciate it! -- Bharti Parihar, HT (ASCP)CM From JosefaNava at texashealth.org Mon Jun 18 06:22:35 2018 From: JosefaNava at texashealth.org (Nava, Josefa) Date: Mon, 18 Jun 2018 11:22:35 +0000 Subject: [Histonet] Histonet Digest, Vol 175, Issue 15 In-Reply-To: References: Message-ID: unsubscribe -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Saturday, June 16, 2018 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 175, Issue 15 [EXTERNAL] Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=tAAbe3VWSXg4_eMSItYKuRlnp5vCb2OrQZQHnRXk_fc&r=h0EwiXOa8WlT7rjYHe7TZnXzwRXmblPlGcETNHHbbyE&m=RCrqLpf9kzw7THpxjY2pykyQ5Qb5lW6jfciAqUZ2Ho4&s=DvuYiGLJ75JPR6bFHU4b-c7vOdBOQgdz99K76SsRs_c&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Staining Protocol for Chromosome Counting in Plants (Bharti Parihar) ---------------------------------------------------------------------- Message: 1 Date: Fri, 15 Jun 2018 15:09:00 -0700 From: Bharti Parihar To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Staining Protocol for Chromosome Counting in Plants Message-ID: Content-Type: text/plain; charset="UTF-8" Hi ya'll. If there are any individuals out there that have done karyotyping, I need to try a protocol to get a chromosome count on a desert plant. We have germinated seeds with long roots, so sample size shouldn't be an issue. Tried a Orcein stain, but the results are quite lacking and it may not be the right stain for this plant. Gonna do a bit of modification with the fixative to specimen ratio to see if that helps, but it's possible this just isn't the right stain. After all, the stain ordered is for Arabidopsis, which is not our research plant. This is my first time doing plant histo, I'm used to human tissue, so I really don't know which stain to try. Anyone with any help/advice to offer, I would immensely appreciate it! -- Bharti Parihar, HT (ASCP)CM ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=tAAbe3VWSXg4_eMSItYKuRlnp5vCb2OrQZQHnRXk_fc&r=h0EwiXOa8WlT7rjYHe7TZnXzwRXmblPlGcETNHHbbyE&m=RCrqLpf9kzw7THpxjY2pykyQ5Qb5lW6jfciAqUZ2Ho4&s=DvuYiGLJ75JPR6bFHU4b-c7vOdBOQgdz99K76SsRs_c&e= ------------------------------ End of Histonet Digest, Vol 175, Issue 15 ***************************************** The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From JosefaNava at texashealth.org Mon Jun 18 06:23:03 2018 From: JosefaNava at texashealth.org (Nava, Josefa) Date: Mon, 18 Jun 2018 11:23:03 +0000 Subject: [Histonet] Histonet Digest, Vol 175, Issue 15 In-Reply-To: References: Message-ID: unsubscribe -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Saturday, June 16, 2018 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 175, Issue 15 [EXTERNAL] Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=tAAbe3VWSXg4_eMSItYKuRlnp5vCb2OrQZQHnRXk_fc&r=h0EwiXOa8WlT7rjYHe7TZnXzwRXmblPlGcETNHHbbyE&m=RCrqLpf9kzw7THpxjY2pykyQ5Qb5lW6jfciAqUZ2Ho4&s=DvuYiGLJ75JPR6bFHU4b-c7vOdBOQgdz99K76SsRs_c&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Staining Protocol for Chromosome Counting in Plants (Bharti Parihar) ---------------------------------------------------------------------- Message: 1 Date: Fri, 15 Jun 2018 15:09:00 -0700 From: Bharti Parihar To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Staining Protocol for Chromosome Counting in Plants Message-ID: Content-Type: text/plain; charset="UTF-8" Hi ya'll. If there are any individuals out there that have done karyotyping, I need to try a protocol to get a chromosome count on a desert plant. We have germinated seeds with long roots, so sample size shouldn't be an issue. Tried a Orcein stain, but the results are quite lacking and it may not be the right stain for this plant. Gonna do a bit of modification with the fixative to specimen ratio to see if that helps, but it's possible this just isn't the right stain. After all, the stain ordered is for Arabidopsis, which is not our research plant. This is my first time doing plant histo, I'm used to human tissue, so I really don't know which stain to try. Anyone with any help/advice to offer, I would immensely appreciate it! -- Bharti Parihar, HT (ASCP)CM ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=tAAbe3VWSXg4_eMSItYKuRlnp5vCb2OrQZQHnRXk_fc&r=h0EwiXOa8WlT7rjYHe7TZnXzwRXmblPlGcETNHHbbyE&m=RCrqLpf9kzw7THpxjY2pykyQ5Qb5lW6jfciAqUZ2Ho4&s=DvuYiGLJ75JPR6bFHU4b-c7vOdBOQgdz99K76SsRs_c&e= ------------------------------ End of Histonet Digest, Vol 175, Issue 15 ***************************************** The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From criley at dpspa.com Mon Jun 18 07:13:20 2018 From: criley at dpspa.com (Charles Riley) Date: Mon, 18 Jun 2018 08:13:20 -0400 Subject: [Histonet] Anti-RET Message-ID: Does anyone out there run the Anti-RET IHC from ABCAM (ab134100) on the Leica bond platform. I am about to work it up on out system and want a good starting point rather than testing every option and wasting time and money. 1. Do you use any enzyme treatment 2. Do you use ER1 or ER2 and for what times? 3. What do you find to be a good dilution ratio? Any assistance will be greatly appreciated -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From c.tague at Pathologyarts.com Mon Jun 18 08:41:02 2018 From: c.tague at Pathologyarts.com (Curt) Date: Mon, 18 Jun 2018 13:41:02 +0000 Subject: [Histonet] benchmark special stain free sol Message-ID: <9C8F910F72893643B3C3793C3D67132B83E34E24@PATHOLOGYSERVER.pathologyarts.local> I have 1 bottle of LCS and 1.5 bottle of depar solution for the benchmark special stain platforms, send me a fedex or ups number if you want them. Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. From victor_tobias at comcast.net Mon Jun 18 09:06:35 2018 From: victor_tobias at comcast.net (victor_tobias at comcast.net) Date: Mon, 18 Jun 2018 07:06:35 -0700 Subject: [Histonet] Histonet Digest, Vol 175, Issue 15 Message-ID: To unsubscribe, please follow this link http://lists.utsouthwestern.edu/mailman/listinfo/histonet Victor Sent from Xfinity Connect Mobile App ------ Original Message ------ From: Nava, Josefa via Histonet To: histonet at lists.utsouthwestern.edu Sent: June 18, 2018 at 4:25 AM Subject: Re: [Histonet] Histonet Digest, Vol 175, Issue 15 unsubscribe -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Saturday, June 16, 2018 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 175, Issue 15 [EXTERNAL] Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=tAAbe3VWSXg4_eMSItYKuRlnp5vCb2OrQZQHnRXk_fc&r=h0EwiXOa8WlT7rjYHe7TZnXzwRXmblPlGcETNHHbbyE&m=RCrqLpf9kzw7THpxjY2pykyQ5Qb5lW6jfciAqUZ2Ho4&s=DvuYiGLJ75JPR6bFHU4b-c7vOdBOQgdz99K76SsRs_c&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest. .." Today's Topics: 1. Staining Protocol for Chromosome Counting in Plants (Bharti Parihar) ---------------------------------------------------------------------- Message: 1 Date: Fri, 15 Jun 2018 15:09:00 -0700 From: Bharti Parihar To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Staining Protocol for Chromosome Counting in Plants Message-ID: Content-Type: text/plain; charset="UTF-8" Hi ya'll. If there are any individuals out there that have done karyotyping, I need to try a protocol to get a chromosome count on a desert plant. We have germinated seeds with long roots, so sample size shouldn't be an issue. Tried a Orcein stain, but the results are quite lacking and it may not be theright stain for this plant. Gonna do a bit of modification with the fixative to specimen ratio to see if that helps, but it's possible this just isn't the right stain. After all, the stain ordered is for Arabidopsis, which is not our research plant. This is my first time doing plant histo, I'm us ed to human tissue, so I really don't know which stain to try. Anyone with any help/advice to offer, I would immensely appreciate it! -- Bharti Parihar, HT (ASCP)CM ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=tAAbe3VWSXg4_eMSItYKuRlnp5vCb2OrQZQHnRXk_fc&r=h0EwiXOa8WlT7rjYHe7TZnXzwRXmblPlGcETNHHbbyE&m=RCrqLpf9kzw7THpxjY2pykyQ5Qb5lW6jfciAqUZ2Ho4&s=DvuYiGLJ75JPR6bFHU4b-c7vOdBOQgdz99K76SsRs_c&e= ------------------------------ End of Histonet Digest, Vol 175, Issue 15 ***************************************** The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If yo u are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Linda.Margraf at cookchildrens.org Mon Jun 18 11:14:15 2018 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Mon, 18 Jun 2018 16:14:15 +0000 Subject: [Histonet] Histotech positions Message-ID: <5eaac480b2c44a3aa7ca967509eff7fc@MBX10.CCHCS.LDAP> Here is a message I am posting for Carolyn....... Folio Biosciences and Conversant Bio have merged to create the life science industry's premier provider of research services and biospecimen solutions. We're looking for 2 stand out Histology Technicians, who will prepare histologic slides from tissue sections for microscopic examination by staff pathologists. This position is Monday - Friday on day shift. Are you someone who is moved by utilizing your histology skills in a growing company? To be successful in this job you must be focused, methodical, precise and able to work collaboratively with a variety of groups. You must be Certified or eligible for Board of Certification (BOC) by the American Society of Clinical Pathologists (ASCP) and have a minimum of one year of experience as a Histology Technician with competency in the areas of embedding, microtomy and special stains, with a knowledge of and/or experience with immunohistochemistry and cryosectioning procedures preferred. You should also possess excellent interpersonal, verbal and written communication skills. You must be able to work in a team that values open and honest communication, embraces challenge, innovation and continuous improvement, works hard and loves to have fun, is fast-paced and displays a can-do attitude. If this is you and you can prove it, you could be a great fit for our team. We offer excellent pay and benefits, an unbeatable work environment, and the chance to make a difference in the lives of patients. We hire based on talent and fit. Apply only if you meet our performance and ability guidelines. You can find us at www.conversantbio.com EEO/ADA For more information on Folio Conversant, visit www.conversantbio.com Regards, Carolyn Dodson Director, Human Resources From thisisann at aol.com Mon Jun 18 14:37:05 2018 From: thisisann at aol.com (Ann Specian) Date: Mon, 18 Jun 2018 15:37:05 -0400 Subject: [Histonet] Proscia Message-ID: <164146699f3-c8f-2bcf@webjas-vae091.srv.aolmail.net> Has anyone used Proscia for digital imaging? Comments? From lblazek at digestivespecialists.com Tue Jun 19 07:04:46 2018 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Tue, 19 Jun 2018 08:04:46 -0400 Subject: [Histonet] job opening Message-ID: <5A2BD13465E061429D6455C8D6B40E392A83301A91@IBMB7Exchange.digestivespecialists.com> We're looking for a Histology Technician, who will prepare histology slides from tissue sections for microscopic examination by staff pathologists. This position is Monday - Friday day shift position. We are a growing company that has just opened a brand new facility, with windows! You must be Certified or eligible for Board of Certification (BOC) by the American Society of Clinical Pathologists (ASCP) and have a minimum of one year of experience as a Histology Technician with competency in the areas of processing, embedding, microtomy and a minimum number of special stains and with a basic knowledge of immunohistochemistry. We work hard to encourage team values, open honest communication and a system of continuous quality improvement. For consideration for this position, please send a cover letter and your resume to DSIemployment at digestivespecialists.com and place your name on the subject line of the email. Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com From criley at dpspa.com Tue Jun 19 07:39:49 2018 From: criley at dpspa.com (Charles Riley) Date: Tue, 19 Jun 2018 08:39:49 -0400 Subject: [Histonet] Microscope objectives Message-ID: Are all microscope objectives universal between brands or do you have to buy the objectives from the company that manufactured it? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From rjbuesa at yahoo.com Tue Jun 19 08:44:41 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Tue, 19 Jun 2018 13:44:41 +0000 (UTC) Subject: [Histonet] Microscope objectives In-Reply-To: References: Message-ID: <1974902255.1745654.1529415881408@mail.yahoo.com> Not all objectives "are created equally"."In the beginning" (as in Genesis) the objective "was created" to be used on microscopes with essentially two different focal distances, i.e. 160mm and 170mm. By the 1950's the ? corrected objective appeared to simplify somewhat the situation BUT every manufacturer tried that all their objectives were "par-focal" meaning that you could switch from one objective to the next and the virtual image remained "in focus".That is the crux of the issue: a virtual image permanently in focus, and that cannot be obtained between objectives from different manufacturers because their sizes (lengths) are different, and their focal points may also differ.So, putting it simply, unless you are willing to refocus with either your "macro" or "micro" knob (focusing wheel) you will need to buy all your objectives from the same manufacturer (brand).Ren? On Tuesday, June 19, 2018 8:58 AM, Charles Riley via Histonet wrote: Are all microscope objectives universal between brands or do you have to buy the objectives from the company that manufactured it? -- Charles Riley BS? HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Tue Jun 19 09:16:31 2018 From: patpxs at gmail.com (Patpxs) Date: Tue, 19 Jun 2018 07:16:31 -0700 Subject: [Histonet] Microscope objectives In-Reply-To: <1974902255.1745654.1529415881408@mail.yahoo.com> References: <1974902255.1745654.1529415881408@mail.yahoo.com> Message-ID: Also, the manufacturers make the threads different so what works on one brand won?t work on another. They even change the threading between models so you have to buy new objectives. Paula Sent from my iPhone > On Jun 19, 2018, at 6:44 AM, Rene J Buesa via Histonet wrote: > > Not all objectives "are created equally"."In the beginning" (as in Genesis) the objective "was created" to be used on microscopes with essentially two different focal distances, i.e. 160mm and 170mm. By the 1950's the ? corrected objective appeared to simplify somewhat the situation BUT every manufacturer tried that all their objectives were "par-focal" meaning that you could switch from one objective to the next and the virtual image remained "in focus".That is the crux of the issue: a virtual image permanently in focus, and that cannot be obtained between objectives from different manufacturers because their sizes (lengths) are different, and their focal points may also differ.So, putting it simply, unless you are willing to refocus with either your "macro" or "micro" knob (focusing wheel) you will need to buy all your objectives from the same manufacturer (brand).Ren? > > On Tuesday, June 19, 2018 8:58 AM, Charles Riley via Histonet wrote: > > > Are all microscope objectives universal between brands or do you have to > buy the objectives from the company that manufactured it? > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Tue Jun 19 10:42:35 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 19 Jun 2018 11:42:35 -0400 Subject: [Histonet] Find some shade and a cool drink and please take a minute to peruse the RELIA Histology Careers Bulletin for 6-19-2018. Message-ID: <000001d407e4$25001f90$6f005eb0$@earthlink.net> Hi Histonetters, How are you? I hope you are having a great week like me! The phones haven?t stopped ringing Things are heating up just like the temps! So I thought I would shoot you a quick email and let you know about some new opportunities that came in late last week and over the weekend. I am really excited because these are great opportunities with stable companies experiencing growth, some are even RELIA EXCLIUSIVES!!! If you have vacations already planned my clients understand. You won?t have to change your plans if you change jobs. I PROMISE!!!!! So Histonetters if you have a second grab a patch of shade and a cool beverage and take a second to browse the list of my current openings. I have histology leadership opportunities in: ? California ? Ohio ? Maine I have histology technician/technologist opportunities in: ? Texas ? Ohio ? California ? North Carolina ? The Florida Panhandle All of my clients offer excellent compensation, benefits. Some offer relocation assistance and or sign on bonuses. All of these jobs are full time & permanent. If you or anyone you know is interested in hearing more about any of these opportunities please contact me. If I place someone you refer to me you will earn a referral bonus!! I can be reached ASAP on my cell at 407-353-5070 or e-mail me at relia1 at earthlink.net or call me toll free at 866-607-3542. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From naira.margaryan at hsc.wvu.edu Thu Jun 21 12:00:26 2018 From: naira.margaryan at hsc.wvu.edu (Margaryan, Naira) Date: Thu, 21 Jun 2018 17:00:26 +0000 Subject: [Histonet] Polyclonal Rabbit Anti-Cytokeratin Message-ID: Hello, I was asked to do IHC using the Polyclonal Rabbit Anti-Cytokeratin, Wide Spectrum Screening, Polyclonal, Unconjugated, Ig fraction, (Z062201-2). Ca anyone help me with AR to use and concentration to start. Thanks in advance, Naira From jriggleman at globusmedical.com Thu Jun 21 13:59:47 2018 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Thu, 21 Jun 2018 18:59:47 +0000 Subject: [Histonet] MMA or Epoxy Embedding Message-ID: Hi Everyone, I am trying to embed screws in plastic (in the hopes to see breakage in the screw, etc). Right now I use specifically a mixture of methyl methacrylate, poly methyl methacrylate, and benzoyl peroxide. However this usually takes a few weeks to embed/dry. I am looking for something a little faster (perhaps a week max?). Thank You, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From michael.gudo at morphisto.de Thu Jun 21 15:54:48 2018 From: michael.gudo at morphisto.de (Michael Gudo (Morphisto GmbH)) Date: Thu, 21 Jun 2018 22:54:48 +0200 Subject: [Histonet] MMA or Epoxy Embedding In-Reply-To: References: Message-ID: Dear Jessica, the best way to embed screws or any other implant material or bones or teeth or any compounds is to Technovit 7200. It a single component Acrylate that hardens in blue and white light within a few hours. We are the exclusive distributor for all Histology Technovits from Kulzer, so please contact me personally for further details regarding price, shipping and detailed instructions. We can ship worldwide and have some Kind regards Michael P.S. Here is a short description: https://www.morphisto.de/en/resin-histology/technovit-resins/technovit-7200/ > Am 21.06.2018 um 20:59 schrieb Jessica Riggleman via Histonet : > > Hi Everyone, > I am trying to embed screws in plastic (in the hopes to see breakage in the screw, etc). Right now I use specifically a mixture of methyl methacrylate, poly methyl methacrylate, and benzoyl peroxide. However this usually takes a few weeks to embed/dry. I am looking for something a little faster (perhaps a week max?). > > Thank You, > Jessica > > > _____________________________________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > > Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From tbraud at holyredeemer.com Fri Jun 22 12:32:21 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 22 Jun 2018 17:32:21 +0000 Subject: [Histonet] broken screw in MMA Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF5FC4F@HRHEX02-HOS.holyredeemer.local> Just an idea. Why not use SEM? It seems like it would be so much easier. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 2. MMA or Epoxy Embedding (Jessica Riggleman) Date: Thu, 21 Jun 2018 18:59:47 +0000 From: Jessica Riggleman Subject: [Histonet] MMA or Epoxy Embedding Hi Everyone, I am trying to embed screws in plastic (in the hopes to see breakage in the screw, etc). Right now I use specifically a mixture of methyl methacrylate, poly methyl methacrylate, and benzoyl peroxide. However this usually takes a few weeks to embed/dry. I am looking for something a little faster (perhaps a week max?). Thank You, Jessica Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: From saby_joseph_a at yahoo.com Fri Jun 22 14:13:39 2018 From: saby_joseph_a at yahoo.com (Joseph Saby) Date: Fri, 22 Jun 2018 19:13:39 +0000 (UTC) Subject: [Histonet] broken screw in MMA In-Reply-To: <48E053DDF6CE074DB6A7414BA05403F8ECF5FC4F@HRHEX02-HOS.holyredeemer.local> References: <48E053DDF6CE074DB6A7414BA05403F8ECF5FC4F@HRHEX02-HOS.holyredeemer.local> Message-ID: <1060887744.1005192.1529694819480@mail.yahoo.com> Talk with the people at EXAKT technologies.? They have plastice specially designed just for samples like that. Joe On Friday, June 22, 2018, 1:47:38 PM EDT, Terri Braud via Histonet wrote: Just an idea.? Why not use SEM?? It seems like it would be so much easier. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal ? 2. MMA or Epoxy Embedding (Jessica Riggleman) Date: Thu, 21 Jun 2018 18:59:47 +0000 From: Jessica Riggleman Subject: [Histonet] MMA or Epoxy Embedding Hi Everyone, I am trying to embed screws in plastic (in the hopes to see breakage in the screw, etc). Right now I use specifically a mixture of methyl methacrylate, poly methyl methacrylate, and benzoyl peroxide. However this usually takes a few weeks to embed/dry. I am looking for something a little faster (perhaps a week max?). Thank You, Jessica Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at dpspa.com Mon Jun 25 12:11:59 2018 From: criley at dpspa.com (Charles Riley) Date: Mon, 25 Jun 2018 13:11:59 -0400 Subject: [Histonet] RET PTC Antibody Message-ID: Does anyone use RET antibody to test for Papillary thyroid carcinoma? If so who do you purchase the antibody from. We are looking to add it to our panel along with hbme, gal-3, and BRAF. The one antibody I purchased is not highlighting the target cells the way our pathologists hoped and they want me to look into another vendor -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From relia1 at earthlink.net Tue Jun 26 13:11:54 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 26 Jun 2018 14:11:54 -0400 Subject: [Histonet] Posting for a friend. Submissions requested for NSH's blog "Fixation on Histology" Message-ID: <009501d40d79$2b111250$813336f0$@earthlink.net> Hi Histonetters! If you have something you would like to share to the NSH blog ?Fixation on Histology here is the link for some topic ideas: https://www.surveymonkey.com/r/DCTYBQ7 I am posting this for a friend here is the original message: Hello! We are looking for submissions for NSH?s blog, Fixation on Histology, so I was wondering if anyone would be interested in writing something, or knew someone who would be interested in submitting? Let me know! Thanks ? Natalie Paskoski Project Coordinator National Society for Histotechnology 3545 Ellicott Mills Drive Ellicott City, MD 21043 Phone | 443.535.4064 Fax | 443.535.4055 natalie at nsh.org www.nsh.org |www.histoconvention.org Visit NSH?s new blog, Fixation on Histology Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From relia1 at earthlink.net Wed Jun 27 10:29:25 2018 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 27 Jun 2018 11:29:25 -0400 Subject: [Histonet] Stay cool and Beat the Heat this Summer!! Message-ID: <000001d40e2b$a168ce90$e43a6bb0$@earthlink.net> Hello Histonetters, I hope you are enjoying your Summer!!! I have a quick question for you **What are you doing to beat the heat??** I?m spending a lot of time in the AC and drinking lots and lots of water!! As a Florida Girl here are some of my other strategies: ? I carry a folding fan and a small cotton washcloth ? Freeze about 2 inches of water in the bottom of the bottle and fill it right before you leave. ? Freeze fruit and veggies in water and use them as ice cubes. I would love to hear what you are doing to stay cool this summer. So if you have a minute please shoot me back an email and let me know. It is pretty hot here in Orlando already it?s in the 90?s during the day and we don?t drop below 80 before 11pm at night!!! I can use all of the help I can get!!! The only thing hotter than the temps are my phone lines!! My phones are ringing off the hook with exciting opportunities. Please take a look at my current openings. All of these positions are full time permanent positions with excellent salaries and great benefits. Some of these clients offer generous SIGN ON BONUSES and most of them are RELIA EXCLUSIVES!! My current openings are located in: ? California ? Texas, ? North Carolina ? Alabama ? Ohio Shoot me an email for more info on any of these opportunities. I can?t post any more info publicly ? I don?t want another recruiter to poach an opportunity away from me or YOU!! We have new opportunities coming in daily!! If you or anyone you know might be interested in any of these opportunities please contact me ASAP on my cell at 407-353-5070 or I can be reached at relia1 at earthlink.net or toll free at 866-607-3542. Refer a friend earn a bonus $$$ Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From c.tague at Pathologyarts.com Wed Jun 27 11:55:26 2018 From: c.tague at Pathologyarts.com (Curt) Date: Wed, 27 Jun 2018 16:55:26 +0000 Subject: [Histonet] HP on Benchmark Ultra Message-ID: <9C8F910F72893643B3C3793C3D67132B83E817A4@PATHOLOGYSERVER.pathologyarts.local> Any input is appreciated... We're running HP with Biocares BC7 clone. The stain works well but maybe too well, it seems to stain a variety bacteria as opposed to just the HP rods... and sometimes it gets a little "dirty" with some staining in the mucus as well... I think the first thing we'll try to do is reduce some retrieval times... basically lighten things up but I really want to get other opinions... some of our pathologists think it's suboptimal with all the other staining but I want to hear what others see, are you all seeing other seemingly nonspecific staining too? Maybe it's just a bad control, suboptimal... maybe we can dilute it down an little, reduce times... Really wish I could attach a picture, it's not terrible but not perfect. Any thoughts? Thanks, Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. From JosephE at mcclainlab.com Thu Jun 28 13:02:36 2018 From: JosephE at mcclainlab.com (Joseph Esposito) Date: Thu, 28 Jun 2018 18:02:36 +0000 Subject: [Histonet] Bausch & Lomb Microscope Manual Message-ID: My employer recently purchased a Bausch & Lomb binocular microscope that he intends to give to a friend. He would like a pdf of the instruction manual to give them with the scope, however after conducting several web searches we were unable to find the correct instruction manual for the microscope that he purchased. After looking over the microscope for a bit, we were unable to find a model number, but we were able to find what seemed to be a serial number printed on the microscope. The serial number is 16033-443. Hopefully this helps to identify the model of the scope. I was told it was a Bausch & Lomb Dynoptic, but it has a mirror as the light source instead of having a light source in the base of the microscope. If anyone recognizes the description of this microscope and knows where we can get a pdf of the instruction manual for it, that would be greatly appreciated. Joseph A. Esposito McClain Laboratories, LLC. (631) 361- 4000 From Linda.Margraf at cookchildrens.org Thu Jun 28 17:49:38 2018 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Thu, 28 Jun 2018 22:49:38 +0000 Subject: [Histonet] AFB control tissue Message-ID: <4465706f97824f1f862bd7a250aaa827@MBX10.CCHCS.LDAP> Histonetters, Here is a message I am posting for Chrissy. Please email her if you can help. Thanks! I am in need of (+) AFB control block tissue if anyone has some they can spare? Thanks in advance! Chrissy - St. Mary's Healthcare, Amsterdam NY Christine.Altemus at ascension.org From plucas at biopath.org Fri Jun 29 14:34:01 2018 From: plucas at biopath.org (Paula) Date: Fri, 29 Jun 2018 12:34:01 -0700 Subject: [Histonet] Disposing Paraffin Message-ID: <000901d40fe0$2358a550$6a09eff0$@biopath.org> Hello all.this is an on-going topic with our lab. If anyone can comment about your experience with paraffin waste, I appreciate that in advance. Does your lab or state requirements classify paraffin waste as "hazardous, bio-hazardous, RCRA or other"? Do you dispose as part of Red Bag trash for autoclave or tissue pathology waste or other hazardous waste for incineration? Paula Lab Manager for Biopath Medical Group in Fountain Valley CA From cyang at sterlingpath.com Fri Jun 29 19:33:49 2018 From: cyang at sterlingpath.com (C Yang) Date: Sat, 30 Jun 2018 00:33:49 +0000 (UTC) Subject: [Histonet] Seeking Histotech References: <952661164.595692.1530318829355.ref@mail.yahoo.com> Message-ID: <952661164.595692.1530318829355@mail.yahoo.com> Sterling Pathology National Laboratories located in Seal Beach, California is seeking histotech for part time, per diem help in doing mostly biopsy specimens. Full time may also be a consideration. If interested please reply with resume to hr at sterlingpath.com Thank you and my best regards. Changgao Yang, MDMedical Director From rjbuesa at yahoo.com Sat Jun 30 07:20:43 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Sat, 30 Jun 2018 12:20:43 +0000 (UTC) Subject: [Histonet] Disposing Paraffin In-Reply-To: <000901d40fe0$2358a550$6a09eff0$@biopath.org> References: <000901d40fe0$2358a550$6a09eff0$@biopath.org> Message-ID: <827573194.719845.1530361243114@mail.yahoo.com> We incinerated itRen? On ?Friday?, ?June? ?29?, ?2018? ?03?:?49?:?50? ?PM, Paula via Histonet wrote: Hello all.this is an on-going topic with our lab. If anyone can comment about your experience with paraffin waste, I appreciate that in advance. Does your lab or state requirements classify paraffin waste as "hazardous, bio-hazardous, RCRA or other"?? Do you dispose as part of Red Bag trash for autoclave or tissue pathology waste or other hazardous waste for incineration? Paula Lab Manager for Biopath Medical Group in Fountain Valley CA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet | | Virus-free. www.avast.com | From Desiree.Ledee at alegent.org Sat Jun 30 14:03:42 2018 From: Desiree.Ledee at alegent.org (Ledee,Desiree Y) Date: Sat, 30 Jun 2018 19:03:42 +0000 Subject: [Histonet] Disposing Paraffin In-Reply-To: <827573194.719845.1530361243114@mail.yahoo.com> References: <000901d40fe0$2358a550$6a09eff0$@biopath.org>, <827573194.719845.1530361243114@mail.yahoo.com> Message-ID: We incinerate our tissue blocks. What about paraffin waste from tissue processors Get Outlook for iOS ________________________________ From: Rene J Buesa via Histonet Sent: Saturday, June 30, 2018 7:20:43 AM To: histonet at lists.utsouthwestern.edu; Paula Subject: Re: [Histonet] Disposing Paraffin CAUTION: This email is not from a CHI source. Only click links or open attachments you know are safe. ...................................................................... We incinerated itRen? On ?Friday?, ?June? ?29?, ?2018? ?03?:?49?:?50? ?PM, Paula via Histonet wrote: Hello all.this is an on-going topic with our lab. If anyone can comment about your experience with paraffin waste, I appreciate that in advance. Does your lab or state requirements classify paraffin waste as "hazardous, bio-hazardous, RCRA or other"? Do you dispose as part of Red Bag trash for autoclave or tissue pathology waste or other hazardous waste for incineration? Paula Lab Manager for Biopath Medical Group in Fountain Valley CA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=YFhW2PYwN3hsZhoCqLOPHsIEIPQ6qDXkZ40AlEYUG9c&r=T2dmrnFWSvsDOLewkVlIOSwd73D0unldueOGYSGHi6w&m=JkNbVgUG4XTDo5DGwO1g2OwqinxhYFqHDscLuv4-cnY&s=-EOimM4q8Gsj3osAe5nV4dpZHqIYuRUDwtlIaq0xYEk&e= | | Virus-free. www.avast.com | _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=YFhW2PYwN3hsZhoCqLOPHsIEIPQ6qDXkZ40AlEYUG9c&r=T2dmrnFWSvsDOLewkVlIOSwd73D0unldueOGYSGHi6w&m=JkNbVgUG4XTDo5DGwO1g2OwqinxhYFqHDscLuv4-cnY&s=-EOimM4q8Gsj3osAe5nV4dpZHqIYuRUDwtlIaq0xYEk&e= ________________________________ The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. CHI Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections.