From relia1 at earthlink.net Thu Jul 5 09:35:47 2018 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 5 Jul 2018 10:35:47 -0400 Subject: [Histonet] Histotech needed in Atlanta area. Full time! Permanent! Day Shift ! Can you help? Message-ID: <00c501d4146d$76b06710$64113530$@earthlink.net> Hi Histonetters, How are you? I hope you had a Safe and Happy 4th of July! I have an exciting opportunity to share and if you aren?t interested maybe you know someone who might be! I have been engaged on an exclusive search by one of my best clients located in Atlanta, GA that is in need of a: Full Time Day Shift Grossing Histotech!! This is a full time permanent position in a well-established large GI practice. My client is looking for a strong histotech that is ASCP certified HT/HTL, CLIA qualified to gross with strong routine histology and grossing experience. They are offering an excellent compensation package. The help I need from you Histonetters is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them or pass their contact information to me? *remember if I place someone you refer to me you will earn a referral bonus! If you are interested in this position please contact me ASAP on my cell/text 407-353-5070 or toll free at 866-607-3542 or via email at relia1 at earthlink.net If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won?t release it to anyone without your permission. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From naira.margaryan at hsc.wvu.edu Thu Jul 5 11:58:31 2018 From: naira.margaryan at hsc.wvu.edu (Margaryan, Naira) Date: Thu, 5 Jul 2018 16:58:31 +0000 Subject: [Histonet] Coverslipper Message-ID: Happy Thursday, We would like to sell our slightly used but in excellent condition Dako Coverslipper which was purchased in 2010 $10K (purchase price was $ 25K). The instrument can handle up to 600 slides per hour making it one of the fastest on the market. In addition to the flexibility of the Coverslipper it is easy and straightforward to operate and cleaning and maintenance is simple to do. It is small enough to fit into fume cabinets, easy to move around and accepts a variety of commercial mounting media. Will take any offer, Naira From yesyes at comcast.net Sat Jul 7 13:29:13 2018 From: yesyes at comcast.net (Mary Ann) Date: Sat, 7 Jul 2018 14:29:13 -0400 Subject: [Histonet] Prostate biopsy Message-ID: <4E630275-AD2A-4F6A-A29C-333847E2A572@comcast.net> I have an issue with our prostate needle biopsy chipping out of the block on sectioning. One block out of 12 will demonstrate this phenomenon. The block will section well then a section with chip right out. Sent from my iPhone From edmartin26 at gmail.com Sun Jul 8 22:52:09 2018 From: edmartin26 at gmail.com (Eddie Martin) Date: Sun, 8 Jul 2018 23:52:09 -0400 Subject: [Histonet] Prostate block chipping out In-Reply-To: References: Message-ID: Hi Mary Ann, It seems that you?re having a problem with either your block assembly or your blade holder assembly. I would have your microtome serviced, it may be the block clamp isn?t tight enough or you have knicks on your blade holder. If you?re block clamp is ok, I would lean more on the blade holder needing to be repaired or replaced. The older blade holder assemblies cost more, as they were fabricated to last longer before giving issues with microtomy. The newer microtome assemblies can easily knick and give thick and thin sections, and in this case giving 1 good section, followed by a section that is chunking out. Hope this helps! Cheers, Eddie Martin, HTL, HT(ASCP), QIHC(ASCP) Histology Technical Supervisor The National Institutes of Health The Clinic Center Department of Laboratory Medicine 10 Clinic Dr Bethesda, MD 20814 > On Jul 8, 2018, at 1:00 PM, histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Prostate biopsy (Mary Ann) > > From: Mary Ann > Subject: [Histonet] Prostate biopsy > Date: July 7, 2018 at 2:29:13 PM EDT > To: histonet at lists.utsouthwestern.edu > > > I have an issue with our prostate needle biopsy chipping out of the block on sectioning. > One block out of 12 will demonstrate this phenomenon. The block will section well then a section with chip right out. > > Sent from my iPhone > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Mon Jul 9 08:37:52 2018 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 9 Jul 2018 09:37:52 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin Are You A Night Owl Or A Morning Person?? Message-ID: <000001d4178a$097dc4b0$1c794e10$@earthlink.net> Hello Histonetters, Sooo. How do you like your shift? Are you a night owl on nights or a morning person on days? If you answered I love my shift and Yes - Congratulations!!! On the other hand if you answered NOT my favorite shift and No I am a night owl on days or a morning person on nights. We need to talk! Histonetters, I need to know if you want to change shifts so I can contact you when a position comes up!! Right now I have a lot of day shift positions but if you are looking for nights let me know so I can be on the lookout!! Every one of my current openings is on Day Shift! Here they are: Here is the Super Sizzlin Hot Spotlight Opportunity! Management - A well-known and respected private histology located in Ohio has engaged RELIA in their search for a Manager. They are looking for an ASCP certified HT/HTL with strong management and troubleshooting skills. The role is flexible depending on the skills of the selected candidate this could be a Histology Manager, Anatomic Pathology Manager or even a Lead Histotech who wants to grow into management role. This is a one of a kind unique opportunity for someone who is really geared towards advancement!! These are the other Sizzling HOT Super Opportunities!! Management - Ohio Lead Tech - Ohio Histotech - Alabama Histotech - California Histotech - North Carolina Histotech - Atlanta, GA Histotech - Kansas City, MO All of these positions are full time and permanent!! My clients offer excellent compensation, benefits and in most cases either relocation or a sign-on bonus. If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I place someone you refer You will earn a referral fee. If you are interested in any of these opportunities CALL /TEXT MY CELL ASAP!!! At 407-353-5070. I can also be reached toll free at the office at 866-607-3542 or at relia1 at earthlink.net to set up a time to talk at your convenience! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From jriggleman at globusmedical.com Mon Jul 9 15:16:09 2018 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Mon, 9 Jul 2018 20:16:09 +0000 Subject: [Histonet] New Bone Stain Message-ID: Hello, I am trying to assess new bone vs. old bone in an animal model. Any suggestions on thick section stains? I have heard Van Gieson's? Thank You, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From jmacdonald at mtsac.edu Mon Jul 9 17:09:00 2018 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Mon, 9 Jul 2018 22:09:00 +0000 Subject: [Histonet] New Bone Stain In-Reply-To: References: Message-ID: von Kossa will stain the mineralized bone black and a basic fuschin counter stain the osteoid will stain deep pink. Get Outlook for iOS ________________________________ From: Jessica Riggleman via Histonet Sent: Monday, July 9, 2018 1:17 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] New Bone Stain Hello, I am trying to assess new bone vs. old bone in an animal model. Any suggestions on thick section stains? I have heard Van Gieson's? Thank You, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michael.gudo at morphisto.de Tue Jul 10 03:03:32 2018 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Tue, 10 Jul 2018 10:03:32 +0200 Subject: [Histonet] New Bone Stain In-Reply-To: References: Message-ID: Hello Jessica, we have a special ?bone stain? staining solution in our portfolio which is exactly for the purpose you described. Osteoid will be stained green or red to dark red, incomplete mineralized bone bone light red or orange yellow and the demarcation zone light green. Its also possible to differentiate lacunae, canaliculae, feathered bone, etc. This special stain was developed for diagnosis of bone diseases and I think it should be suitable for your purpose. If you are interested in this staining kit, please let me know, we have a local dealer in Oklahoma and so it would be easy to deliver the ready-to-use staining solution to you. Kind regards Michael > Am 09.07.2018 um 22:16 schrieb Jessica Riggleman via Histonet : > > Hello, > I am trying to assess new bone vs. old bone in an animal model. Any suggestions on thick section stains? I have heard Van Gieson's? > > Thank You, > Jessica > > > _____________________________________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > > Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From techmana12 at yahoo.com Tue Jul 10 13:47:34 2018 From: techmana12 at yahoo.com (Dorothy Glass) Date: Tue, 10 Jul 2018 18:47:34 +0000 (UTC) Subject: [Histonet] cause of tissue proccessing problem References: <209178005.1813086.1531248454315.ref@mail.yahoo.com> Message-ID: <209178005.1813086.1531248454315@mail.yahoo.com> I have been told something strange from a pathologist, that some tissue in the same block had processing issues. There are conflicting reasoning of what could be the problem.I have been in in Histology as an HTL for over thirty years, and I have never heard this explaination? for tissue not processing correctly within the same cassette.? We are using the blue formalin absorptionpads at the grossing table. Have been for years. Now our new pathologist has said he is seeing blocks with two or more pieces of tissue in them, some fried and some ok in the same block.? My first thoughtwas no way this is happening, either all had processing problem. Not just some of the blocks. They want to say the formalin pads at the grossing station are neutralizing the tissue before process? What does Histonetters think?? Please give suggestions. Dorothy Glass, HTL (ASCP), IHC From techmana12 at yahoo.com Tue Jul 10 13:52:31 2018 From: techmana12 at yahoo.com (Dorothy Glass) Date: Tue, 10 Jul 2018 18:52:31 +0000 (UTC) Subject: [Histonet] Fw: cause of tissue proccessing problem In-Reply-To: <85451333.1816612.1531248541647@mail.yahoo.com> References: <209178005.1813086.1531248454315.ref@mail.yahoo.com> <209178005.1813086.1531248454315@mail.yahoo.com> <85451333.1816612.1531248541647@mail.yahoo.com> Message-ID: <626946214.1813917.1531248751736@mail.yahoo.com> ----- Forwarded Message ----- From: Dorothy Glass To: Histonet Sent: Tuesday, July 10, 2018, 2:49:01 PM EDTSubject: Fw: cause of tissue proccessing problem ----- Forwarded Message ----- From: Dorothy Glass To: Histonet ; Histonetters Histonet Sent: Tuesday, July 10, 2018, 2:47:34 PM EDTSubject: cause of tissue proccessing problem I have been told something strange from a pathologist, that some tissue in the same block had processing issues. There are conflicting reasoning of what could be the problem.I have been in in Histology as an HTL for over thirty years, and I have never heard this explaination? for tissue not processing correctly within the same cassette.? We are using the blue formalin absorptionpads at the grossing table. Have been for years. Now our new pathologist has said he is seeing blocks with two or more pieces of tissue in them, some fried and some ok in the same block.? My first thoughtwas no way this is happening, either all had processing problem. Not just some of the blocks. They want to say the formalin pads at the grossing station are neutralizing the tissue before process? What does Histonetters think?? Please give suggestions. Dorothy Glass, HTL (ASCP), IHC From jriggleman at globusmedical.com Tue Jul 10 15:15:25 2018 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Tue, 10 Jul 2018 20:15:25 +0000 Subject: [Histonet] New Bone Stain References: , Message-ID: Hey Jennifer, Thanks for your help. I have two questions: 1. What is the point of decalcifying if they are in plastic? 2. Did you see the counterstain? Right now only the bone is staining. How long did you leave these in the counterstain for? Thanks! Jessica From: Mac Donald, Jennifer [mailto:jmacdonald at mtsac.edu] Sent: Tuesday, July 10, 2018 4:07 PM To: Jessica Riggleman ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] New Bone Stain I used to do it on plastic sections of in-de-calcified bone biopsies. It?s a silver reaction. Get Outlook for iOS _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. ________________________________________ From: Jessica Riggleman Sent: Tuesday, July 10, 2018 1:01 PM To: Mac Donald, Jennifer; histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] New Bone Stain Thank You, Have you ever tried this on thick histology sections? I have a titanium implant in this section, so thin is impossible. Thank You, Jessica ________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: An ISO 13485 Registered Company Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From: Mac Donald, Jennifer [mailto:jmacdonald at mtsac.edu] Sent: Monday, July 09, 2018 6:09 PM To: Jessica Riggleman ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] New Bone Stain von Kossa will stain the mineralized bone black and a basic fuschin counter stain the osteoid will stain deep pink. Get Outlook for iOS ________________________________________ From: Jessica Riggleman via Histonet Sent: Monday, July 9, 2018 1:17 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] New Bone Stain Hello, I am trying to assess new bone vs. old bone in an animal model. Any suggestions on thick section stains? I have heard Van Gieson's? Thank You, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmacdonald at mtsac.edu Tue Jul 10 15:24:02 2018 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Tue, 10 Jul 2018 20:24:02 +0000 Subject: [Histonet] New Bone Stain In-Reply-To: References: , , Message-ID: Auto corrected on my phone. They were not decalcified. The counterstain was a brilliant red/fuschia in the osteoid. Get Outlook for iOS ________________________________ From: Jessica Riggleman Sent: Tuesday, July 10, 2018 1:16 PM To: Mac Donald, Jennifer; histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] New Bone Stain Hey Jennifer, Thanks for your help. I have two questions: 1. What is the point of decalcifying if they are in plastic? 2. Did you see the counterstain? Right now only the bone is staining. How long did you leave these in the counterstain for? Thanks! Jessica From: Mac Donald, Jennifer [mailto:jmacdonald at mtsac.edu] Sent: Tuesday, July 10, 2018 4:07 PM To: Jessica Riggleman ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] New Bone Stain I used to do it on plastic sections of in-de-calcified bone biopsies. It?s a silver reaction. Get Outlook for iOS _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. ________________________________________ From: Jessica Riggleman Sent: Tuesday, July 10, 2018 1:01 PM To: Mac Donald, Jennifer; histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] New Bone Stain Thank You, Have you ever tried this on thick histology sections? I have a titanium implant in this section, so thin is impossible. Thank You, Jessica ________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: An ISO 13485 Registered Company Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. From: Mac Donald, Jennifer [mailto:jmacdonald at mtsac.edu] Sent: Monday, July 09, 2018 6:09 PM To: Jessica Riggleman ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] New Bone Stain von Kossa will stain the mineralized bone black and a basic fuschin counter stain the osteoid will stain deep pink. Get Outlook for iOS ________________________________________ From: Jessica Riggleman via Histonet Sent: Monday, July 9, 2018 1:17 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] New Bone Stain Hello, I am trying to assess new bone vs. old bone in an animal model. Any suggestions on thick section stains? I have heard Van Gieson's? Thank You, Jessica _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michael.gudo at morphisto.de Wed Jul 11 06:57:51 2018 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Wed, 11 Jul 2018 13:57:51 +0200 Subject: [Histonet] New Bone Stain In-Reply-To: References: Message-ID: <37F9AC6B-0EA4-42F2-BC70-CF34E464B1EC@morphisto.de> Hello Jessica, Hello Natalia, we have discussed the question of bone staining methods in our lab and we would recommend the following stainings, which will work on resin embedded material: (1) For an overview and differentiation of various types of bone the traditional MASSON-GOLDNER or AZAN staining is quite fine, and should be the first try because these stains are more or less easy and fast and the staining solutions are not too expensive. (2) MOVAT-Pentachrome in the original and in the modification after VERHOEFF also gives quite good differentiations of resin embedded bones and shows many details even in different types of bone. We use these staining here in many research projects we do for pharma companies that develop bone replacement materials. (3) van KOSSA is also a very good silver impregnation to show mineralized bone, AND there is a possible combination with the MOVAT Pentachrom-Staining. (4) Alizarinred can also be used to show different gradients of mineralisation; the staining kit contains solutions with several pH-values. (5) Picro-Siriusred and other combinations of picric acid and other stains (picro fuchsin, picro polychrom, picro indigocarmin, and orcein-picroindicocramin can show good differentiations on tissues and cartilage and bone structures. Remark: Sometimes its a bit difficult to establish picric acid based stainings on resin embedded tissues, if the resin has not been completely removed (which is possible with technovit 9100 (and some other MMA?s), but not with technovit 7200). (6) LEVAI-LACZKO's stain provides a good differentiation between mineralized bone matrix, Osteoid and synthetic bone substitutes. (7) A special ?Bone Stain?, published by Villanueva in 2009 (https://www.tandfonline.com/doi/abs/10.3109/10520297409116928 ) works best on fresh material. We have not yet tested it completely on resin embedded tissues, but some of our customers used it successfully. For this stain, a ?staining powder" has to be synthesized from various single stains (basic fuchsin, orange G, fastgreen and azure II) and finally mixed into a ready to use staining solution; we produce the stain powder and ready to use solution here in our lab. (8) Last but not least HEROVICI?s stain might help beacuse it differentiates between new and old collagen structures. Though it was originally developed for diagnostics in wound repair, some of our customers use it to differentiate between mineralized bone, new bone (Osteoid) and synthetic bone substitutes. For all staining procedures on resin embedded material its necessary to edge the surface with citric acid or hydrogen peroxide, depending on the kind of resin you are using. If it is technovit 7200 citric acid should work fine, for technovit 9100 you can also try to remove the resin with methoxyethylacetate and acetone. Please note, that there can be a significant difference in the quality of the stain between Technovit 9100 and Technovit 7200, depending on what you want to see. In general, the stain on Technovit 9100 sections will be more powerful and brilliant than that on Technovit 7200 sections. As mentioned in my previous mail, we have a local dealer in Oklahoma, and if you or somebody else is interested in our ready-to-use stainings kits, just let me know, I will then make the direct contact. Kind regards Michael > Am 10.07.2018 um 22:15 schrieb Jessica Riggleman via Histonet : > > Hey Jennifer, > Thanks for your help. I have two questions: > > 1. What is the point of decalcifying if they are in plastic? > 2. Did you see the counterstain? Right now only the bone is staining. How long did you leave these in the counterstain for? > Thanks! > Jessica > > From: Mac Donald, Jennifer [mailto:jmacdonald at mtsac.edu] > Sent: Tuesday, July 10, 2018 4:07 PM > To: Jessica Riggleman ; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] New Bone Stain > > I used to do it on plastic sections of in-de-calcified bone biopsies. It?s a silver reaction. > > Get Outlook for iOS > > > > _____________________________________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > > Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. > > > ________________________________________ > From: Jessica Riggleman > Sent: Tuesday, July 10, 2018 1:01 PM > To: Mac Donald, Jennifer; histonet at lists.utsouthwestern.edu > Subject: RE: [Histonet] New Bone Stain > > Thank You, > Have you ever tried this on thick histology sections? I have a titanium implant in this section, so thin is impossible. > > Thank You, > Jessica > > ________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > An ISO 13485 Registered Company > > > > > > > > Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. > From: Mac Donald, Jennifer [mailto:jmacdonald at mtsac.edu] > Sent: Monday, July 09, 2018 6:09 PM > To: Jessica Riggleman ; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] New Bone Stain > > von Kossa will stain the mineralized bone black and a basic fuschin counter stain the osteoid will stain deep pink. > > Get Outlook for iOS > > ________________________________________ > From: Jessica Riggleman via Histonet > Sent: Monday, July 9, 2018 1:17 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] New Bone Stain > > Hello, > I am trying to assess new bone vs. old bone in an animal model. Any suggestions on thick section stains? I have heard Van Gieson's? > > Thank You, > Jessica > > > _____________________________________________________________________ > > Jessica Riggleman | Research Associate > > Globus Medical, Inc. > Valley Forge Business Center > 2560 General Armistead Avenue | Audubon, PA 19403 > Ph: (610) 930-1800 ext. 2583 | Fax: > > Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From angela at lji.org Wed Jul 11 08:36:58 2018 From: angela at lji.org (Angela Denn) Date: Wed, 11 Jul 2018 06:36:58 -0700 Subject: [Histonet] Leica Autostainer XL for special stains? Message-ID: Has anybody used Leica's Autostainer for specials? There's a whitepaper on Leica's website describing such use but I'd really like to hear some real world opinions on using it in such a manner. I need an inexpensive, refurbished unit that can handle my heavy hitters: H&E, PAS, and trichrome. I am open to suggestions. *Angela Lamberth Denn* | Histology Technician III *La Jolla Institute for Allergy and Immunology* Histology Core, Room 2328 9420 Athena Circle La Jolla, CA 92037 http://www.lji.org 858.752.2222 From CBird at amli-denton.com Wed Jul 11 12:13:00 2018 From: CBird at amli-denton.com (Cindy Bird) Date: Wed, 11 Jul 2018 12:13:00 -0500 Subject: [Histonet] (no subject) Message-ID: Hello All, I am curious to see what chemical everyone uses to decal bone marrow biopsies? Cindy Bird Anatomical Medical Laboratories, Inc. 1600 Scripture Street Denton, TX 76201 940-384-6210 940-384-6000 Fax 940-565-9588 From Nancy_Schmitt at pa-ucl.com Wed Jul 11 12:27:53 2018 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Wed, 11 Jul 2018 17:27:53 +0000 Subject: [Histonet] p16 and sperm morphology Message-ID: Hello- 1. We are currently running P16 on the Leica Bond Max - are you running or reporting this any differently now that they are 510k FDA approved? 2. Sperm Morphology- are your cytotechnologists reading and reporting out sperm morphology or are your clinical lab techs? Responses appreciated:) Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From lblazek at digestivespecialists.com Wed Jul 11 13:04:42 2018 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Wed, 11 Jul 2018 14:04:42 -0400 Subject: [Histonet] job opening Message-ID: <5A2BD13465E061429D6455C8D6B40E392A85DDCFD1@IBMB7Exchange.digestivespecialists.com> We have an opening for a Histology Technician. This position is Monday - Friday day shift position. We are a growing company that has just opened a brand new facility, with windows! You must be Certified or eligible for Board of Certification (BOC) by the American Society of Clinical Pathologists (ASCP) and have a minimum of one year of experience as a Histology Technician with competency in the areas of processing, embedding, microtomy and with a basic knowledge of immunohistochemistry. We only do a minimum number of special stains. We work hard to encourage team values, open honest communication and a system of continuous quality improvement. For consideration for this position, please send a cover letter and your resume to DSIemployment at digestivespecialists.com and place your name on the subject line of the email, or contact me directly. Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com From boznpl at aol.com Thu Jul 12 09:06:42 2018 From: boznpl at aol.com (Laurie Colbert) Date: Thu, 12 Jul 2018 10:06:42 -0400 Subject: [Histonet] Excelsior Tissue Processor Message-ID: <1648ed0bf09-c8f-962@webjasstg-vab14.srv.aolmail.net> If there is anyone out there who is using the Thermo Fisher Excelsior Tissue processor for biopsies, would you please contact me directly at my personal email?? I have a question about your processing program. Thanks, Laurie Redmond From sbonner at pathregional.com Thu Jul 12 11:10:32 2018 From: sbonner at pathregional.com (Silvia Bonner) Date: Thu, 12 Jul 2018 16:10:32 +0000 Subject: [Histonet] Thermo Scientific NX50 Message-ID: <48b5794d91c94763962daf100ed3e724@MBX04C-ORD1.mex08.mlsrvr.com> Hi Everyone, We are looking to purchase a new cryostat. Do any of you have any experience, good or bad, with the NX50? I would love to hear from you if you do. Thank you, Silvia Bonner, BS, HT(ASCP) CM Histology Supervisor sbonner at pathregional.com Pathologists' Regional Laboratory 1225 Highland Ave Clarkston, WA 99403 (509)758-5576 This email may contain physician, patient and/or attorney material that is confidential or privileged for the sole use of the intended recipient. YOU ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED. If you are not the intended recipient, please notify us immediately by telephone at (208) 746-0516 or (509) 758-5576 and delete all copies. From criley at dpspa.com Thu Jul 12 11:26:21 2018 From: criley at dpspa.com (Charles Riley) Date: Thu, 12 Jul 2018 12:26:21 -0400 Subject: [Histonet] Trouble shooting question Message-ID: My pathologists says that 1 out of 100 (his estimate) of our FNA's cellblocks apears to be "cooked" when looking at the slides. The process has never been changed and all reagents are the same all the time. What is/are the possible cause(s) and what can be done to prevent this 1 out of 100 situation? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From rjbuesa at yahoo.com Thu Jul 12 11:49:36 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 12 Jul 2018 16:49:36 +0000 (UTC) Subject: [Histonet] Trouble shooting question In-Reply-To: References: Message-ID: <377951180.2930091.1531414176756@mail.yahoo.com> Check fixation time and specially IF the Bx was left to dry before fixation.Ren? On ?Thursday?, ?July? ?12?, ?2018? ?12?:?38?:?28? ?PM, Charles Riley via Histonet wrote: My pathologists says that 1 out of 100 (his estimate) of our FNA's cellblocks? apears to be "cooked" when looking at the slides.? The process has never been changed and all reagents are the same all the time. What is/are the possible cause(s) and what can be done to prevent this 1 out of 100 situation? -- Charles Riley BS? HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet | | Virus-free. www.avast.com | From tim.higgins at cddmedical.com Thu Jul 12 13:07:48 2018 From: tim.higgins at cddmedical.com (Tim Higgins) Date: Thu, 12 Jul 2018 18:07:48 +0000 Subject: [Histonet] Subject: Re: Trouble shooting question Message-ID: Hey Charles, I have to agree with Rene. They are either being left out after sampling prior to fixation or possibly coming into contact with air during the processing run. Make sure your solution are covering all the cassettes, don't let them float free inside the processing chamber. Thanks, Tim ------------------------------ Message: 6 Date: Thu, 12 Jul 2018 12:26:21 -0400 From: Charles Riley To: Histo List Subject: [Histonet] Trouble shooting question Message-ID: Content-Type: text/plain; charset="UTF-8" My pathologists says that 1 out of 100 (his estimate) of our FNA's cellblocks apears to be "cooked" when looking at the slides. The process has never been changed and all reagents are the same all the time. What is/are the possible cause(s) and what can be done to prevent this 1 out of 100 situation? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ------------------------------ Message: 7 Date: Thu, 12 Jul 2018 16:49:36 +0000 (UTC) From: Rene J Buesa To: Histo List , Charles Riley Subject: Re: [Histonet] Trouble shooting question Message-ID: <377951180.2930091.1531414176756 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Check fixation time and specially IF the Bx was left to dry before fixation.Ren? On ?Thursday?, ?July? ?12?, ?2018? ?12?:?38?:?28? ?PM, Charles Riley via Histonet wrote: My pathologists says that 1 out of 100 (his estimate) of our FNA's cellblocks? apears to be "cooked" when looking at the slides.? The process has never been changed and all reagents are the same all the time. What is/are the possible cause(s) and what can be done to prevent this 1 out of 100 situation? -- Charles Riley BS? HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet | | Virus-free. www.avast.com | ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 176, Issue 7 **************************************** From gordon at 10db.co.uk Fri Jul 13 05:18:34 2018 From: gordon at 10db.co.uk (Gordon Brown) Date: Fri, 13 Jul 2018 11:18:34 +0100 Subject: [Histonet] Cassette chucks and blade holders Message-ID: As an amateur microscopist I've made a few posts to Histonet over the years and received some very helpful advice on text books etc and I mentioned in my last series of posts that I'm restoring several old microtomes that have come my way. I've been given a box of cassettes for these and wonder if there are any labs out there who have any redundant, or possibly damaged but working cassette chucks and replaceable blade holders that may be suitable for older Leitz and Reichert microtomes? I have three Reicherts and a Leitz but only one blade holder that is suitable and no cassette chucks at all. Happy to consider any possibilities! I'm in the UK but again happy to sort out postage costs etc. Gordon From yesyes at comcast.net Mon Jul 16 11:51:53 2018 From: yesyes at comcast.net (Mary Ann) Date: Mon, 16 Jul 2018 12:51:53 -0400 Subject: [Histonet] Cap PAP Smears Controls Message-ID: <737C63EC-9DED-4EFE-A456-D6720ED666D2@comcast.net> Hello, In preparing for CAP I have a question: Does anyone run a positive PAP control with their run? Sent from my iPhone From cls71877 at gmail.com Mon Jul 16 12:45:30 2018 From: cls71877 at gmail.com (Cristi Rigazio) Date: Mon, 16 Jul 2018 13:45:30 -0400 Subject: [Histonet] Automated Embedder Message-ID: Good morning fellow histo peeps! We have been looking into the automated embedding system. The cutting room is excited about this opportunity, but the grossing personnel have some concerns. Is anyone out there currently using this? Can anyone provide some input on tissue types, implementation, time differences in the use of the cassette inserts, etc.,? thanks, Cristi From tbraud at holyredeemer.com Mon Jul 16 15:56:04 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 16 Jul 2018 20:56:04 +0000 Subject: [Histonet] pap control Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF650F8@HRHEX02-HOS.holyredeemer.local> Are you referring to a Pap stain control? If so, we run a self made buccal smear every day to check for stain quality. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Monday, July 16, 2018 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 176, Issue 9 Today's Topics: 1. Cap PAP Smears Controls (Mary Ann) Message: 1 Date: Mon, 16 Jul 2018 12:51:53 -0400 From: Mary Ann Subject: [Histonet] Cap PAP Smears Controls Hello, In preparing for CAP I have a question: Does anyone run a positive PAP control with their run? From jwwalker at rrmc.org Tue Jul 17 10:49:53 2018 From: jwwalker at rrmc.org (Joe W. Walker, Jr.) Date: Tue, 17 Jul 2018 15:49:53 +0000 Subject: [Histonet] Cap PAP Smears Controls In-Reply-To: <737C63EC-9DED-4EFE-A456-D6720ED666D2@comcast.net> References: <737C63EC-9DED-4EFE-A456-D6720ED666D2@comcast.net> Message-ID: HI Mary Ann, If you are referring to the Pap stain for Pap tests or for Non-Gyn specimens including FNA, you will need to verify daily that the technical quality of the slides. This evaluation should include any preparation for the day, including liquid based, cytospins, direct smears, and cell blocks. The slides should be evaluated to ensure the presentation of the cells, including the staining characteristics are appropriate for the nuclei and cytoplasm of the cells in question. In our lab, we evaluate a slide from each staining run. If we run only Pap tests, then a slide is randomly pulled from the run and evaluated. For non-gyn specimens, we apply the same technique if those specimens types were run on any day. For non-gyn specimens, we also evaluate the Romanowsky stained slides. As a CAP inspector, I have also observed labs who utilize a buckle smear for direct smear preps and also for liquid based preps. They will run them through the stains to ensure that they work as expected before staining patient specimens. I don't think there is one correct way to perform this evaluation but the key is to make sure that you are evaluating each type of prep and the stain that you perform in your lab each day. Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790? F 802.747.6525 joewalker at rrmc.org, www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Our Commitment to our Community: We Listen, We Respect, We Care . . . Always. Joint Commission Accredited | Best Regional Hospital: U.S. News & World Report | Leapfrog Hospital Safety A Rating ANCC Magnet Hospital Designation? | Healthgrades: Excellence Award for Patient Safety | Healthgrades: Outstanding Patient Experience Award -----Original Message----- From: Mary Ann via Histonet Sent: Monday, July 16, 2018 12:52 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Cap PAP Smears Controls Hello, In preparing for CAP I have a question: Does anyone run a positive PAP control with their run? Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Cjwwalker%40rrmc.org%7Cf239c68418344b1509a408d5eb3c9ad3%7C0e55647d438e4a448437e959c3cf2240%7C0%7C0%7C636673567881901096&sdata=4co6i0cX4upQrkw0JOvjUA7TwDrrOouTcXIoWV3d31U%3D&reserved=0 From john.frazier at roche.com Tue Jul 17 14:15:35 2018 From: john.frazier at roche.com (Frazier, John) Date: Tue, 17 Jul 2018 21:15:35 +0200 Subject: [Histonet] Automated Embedder Message-ID: Good afternoon Chris, I am a 6 Sigma Black Belt histology consultant with Ventana/Roche. I have visited many hospital, reference and research histology based labs over my 15 years of lab consulting and 20 years in the lab. In all those years, I have only seen 3 labs with the auto-embedders. 2 of the 3 are back to traditional embedding due to quality, additional time spent at grossing (that is where the tissue is oriented in the cassette) and you are forced to purchase Sakura cassettes. The embedding staff at all 3 sites said that they can embed as fast with better quality. Additionally, the block cuts differently due to the thinness of the cassette. The one remaining lab that I know still has one is Covenant in Kentucky and Mountain View, Calf. I am sure there are others auto-embedders out there but I see about 6 labs/month and the auto-embedded have been out there for at least 8-9 years. The concept sounds very Lean and is probably in the 4 sigma range for embedding errors. Lastly, the certified PA?s in most labs are the hire paid between a histologist and a PA. Where do you want to spend your operational dollars? I am not for or against the product. I am just telling you what I have seen. John Frazier, MT(ASCP), MBA, LSSBB Sent from my iPad On Jul 16, 2018, at 1:45 PM, Cristi Rigazio wrote: Good morning fellow histo peeps! We have been looking into the automated embedding system. The cutting room is excited about this opportunity, but the grossing personnel have some concerns. Is anyone out there currently using this? Can anyone provide some input on tissue types, implementation, time differences in the use of the cassette inserts, etc.,? thanks, Cristi From Jessica.Piche at wtbyhosp.org Wed Jul 18 09:15:14 2018 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Wed, 18 Jul 2018 14:15:14 +0000 Subject: [Histonet] Microtomes Message-ID: <267edd66190c41ad8cba47d25f06d191@WIN12EXCHG13-03.wtbyhosp.org> Good Morning Everyone, We are in the market for new microtomes and was wondering what everyone is using out there? We are looking for semi-automated/automated microtomes. We are demoing the Leica Autocut and the Thermo 355S. I really like the Leica microtome but am not crazy about the control panel. We cut with our water baths to our left and the control panel is in the way. What are other techs doing? I'd appreciate your thoughts. Thank you in advance. Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From boznpl at aol.com Wed Jul 18 10:23:51 2018 From: boznpl at aol.com (Laurie Colbert) Date: Wed, 18 Jul 2018 11:23:51 -0400 Subject: [Histonet] Microtomes In-Reply-To: <267edd66190c41ad8cba47d25f06d191@WIN12EXCHG13-03.wtbyhosp.org> References: <267edd66190c41ad8cba47d25f06d191@WIN12EXCHG13-03.wtbyhosp.org> Message-ID: <164adfd89f6-c92-f5b@webjasstg-vaa01.srv.aolmail.net> We have three Thermo 355S's.? They are nice because you can use them in the automated mode or manual mode.? All of our HT's use the manual mode, so now we just buy the manual version. Laurie Redmond HT (ASCP) Path MD -----Original Message----- From: Piche, Jessica via Histonet To: histonet Sent: Wed, Jul 18, 2018 7:29 am Subject: [Histonet] Microtomes Good Morning Everyone, We are in the market for new microtomes and was wondering what everyone is using out there? We are looking for semi-automated/automated microtomes. We are demoing the Leica Autocut and the Thermo 355S. I really like the Leica microtome but am not crazy about the control panel. We cut with our water baths to our left and the control panel is in the way. What are other techs doing? I'd appreciate your thoughts. Thank you in advance. Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Wed Jul 18 12:56:47 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 18 Jul 2018 17:56:47 +0000 Subject: [Histonet] New Microtome Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF655F0@HRHEX02-HOS.holyredeemer.local> We are using a Leica 2235 and love it. Manual or Auto mode, the control panel is on a moveable pad, so can be used anywhere. It has a foot pedal if you like that too! The techs took to it like a duck to water and it cuts like butter! Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From criley at dpspa.com Wed Jul 18 13:11:00 2018 From: criley at dpspa.com (Charles Riley) Date: Wed, 18 Jul 2018 14:11:00 -0400 Subject: [Histonet] Hazy/cooked cells in H&E Message-ID: Our pathologists have said that we have had a few cases the past few days that are unreadable. They say the cells appear "cooked". Nothing in our process has changed, we have been using the same lot of reagents since the middle of June, and no errors were reported in our processing runs. Do anyone have any ideas to what could be causing this issue? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From jmacdonald at mtsac.edu Wed Jul 18 14:46:12 2018 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Wed, 18 Jul 2018 19:46:12 +0000 Subject: [Histonet] Hazy/cooked cells in H&E In-Reply-To: References: Message-ID: Is anyone using freezing spray? We had this problem and all cases were traced back to the same microtomist. She used the freezing spray excessively and was causing ?freezer burn? to the GI biopsies. Jennifer Get Outlook for iOS ________________________________ From: Charles Riley via Histonet Sent: Wednesday, July 18, 2018 11:12 AM To: Histo List Subject: [Histonet] Hazy/cooked cells in H&E Our pathologists have said that we have had a few cases the past few days that are unreadable. They say the cells appear "cooked". Nothing in our process has changed, we have been using the same lot of reagents since the middle of June, and no errors were reported in our processing runs. Do anyone have any ideas to what could be causing this issue? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cls71877 at gmail.com Wed Jul 18 16:04:48 2018 From: cls71877 at gmail.com (Cristi Rigazio) Date: Wed, 18 Jul 2018 17:04:48 -0400 Subject: [Histonet] Hazy/cooked cells in H&E In-Reply-To: References: Message-ID: <80E920EC-39BC-4750-9C39-13498C8F0504@gmail.com> Have you checked the processors? It is easy to ?mix up? the reagents. Has tissue collection changed? Are the specimens left out longer or placed on paper? Do you use sponges? Any chance the fixation time has been decreased? Sent from my iPhone > On Jul 18, 2018, at 3:46 PM, Mac Donald, Jennifer via Histonet wrote: > > Is anyone using freezing spray? We had this problem and all cases were traced back to the same microtomist. She used the freezing spray excessively and was causing ?freezer burn? to the GI biopsies. > Jennifer > > Get Outlook for iOS > > ________________________________ > From: Charles Riley via Histonet > Sent: Wednesday, July 18, 2018 11:12 AM > To: Histo List > Subject: [Histonet] Hazy/cooked cells in H&E > > Our pathologists have said that we have had a few cases the past few days > that are unreadable. They say the cells appear "cooked". > > Nothing in our process has changed, we have been using the same lot of > reagents since the middle of June, and no errors were reported in our > processing runs. > > Do anyone have any ideas to what could be causing this issue? > > > -- > > Charles Riley BS HT, HTL(ASCP)CM > > Histopathology Coordinator/ Mohs > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting at geisinger.edu Wed Jul 18 22:17:03 2018 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Thu, 19 Jul 2018 03:17:03 +0000 Subject: [Histonet] Sakura glas2 Message-ID: What mounting medium are folks using with their Sakura glas2 coverslip instruments? We just purchased one. Our lab is xylene-free so I want to avoid bringing a xylene-based product in. Sent from Angie's iPhone IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From jmacdonald at mtsac.edu Wed Jul 18 23:28:34 2018 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Thu, 19 Jul 2018 04:28:34 +0000 Subject: [Histonet] Sakura glas2 In-Reply-To: References: Message-ID: We use Permount and an aliphatic hydrocarbon clearing reagent. Jennifer Get Outlook for iOS ________________________________ From: Bitting, Angela K. via Histonet Sent: Wednesday, July 18, 2018 8:18 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Sakura glas2 What mounting medium are folks using with their Sakura glas2 coverslip instruments? We just purchased one. Our lab is xylene-free so I want to avoid bringing a xylene-based product in. Sent from Angie's iPhone IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the e ncrypted e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght at yahoo.com Thu Jul 19 13:15:37 2018 From: tkngflght at yahoo.com (Cheryl) Date: Thu, 19 Jul 2018 13:15:37 -0500 Subject: [Histonet] =?utf-8?q?What_can_y=E2=80=99all_tell_me_about_StatLab?= =?utf-8?q?s_new_ihc?= Message-ID: Hi guys - has anybody done a demo or stepped up as an early adopter? Super curious... Cheryl Please excuse typos-sent from a phone. From WWince at STDOM.com Thu Jul 19 16:01:45 2018 From: WWince at STDOM.com (Wanda Wince) Date: Thu, 19 Jul 2018 16:01:45 -0500 Subject: [Histonet] AUTO: Wanda Wince is out of the office (returning Tue 07/24/2018) Message-ID: I am out of the office from Thu 07/19/2018 until Tue 07/24/2018. If this is a pressing matter, please refer to Mara Hanson @6656 or Sheila Sims @ 6758 Note: This is an automated response to your message "Histonet Digest, Vol 176, Issue 11" sent on 07/19/2018 12:00:01 PM. This is the only notification you will receive while this person is away. From MWhite at mhs.net Mon Jul 23 09:55:59 2018 From: MWhite at mhs.net (White, Marcia) Date: Mon, 23 Jul 2018 14:55:59 +0000 Subject: [Histonet] Disposing Paraffin In-Reply-To: <827573194.719845.1530361243114@mail.yahoo.com> References: <000901d40fe0$2358a550$6a09eff0$@biopath.org> <827573194.719845.1530361243114@mail.yahoo.com> Message-ID: <118e8420956f4c29896f597181a807cb@MHSEX05.mhs.net> We were told that due to the traces of xylene in the paraffin it was considered hazardous so we have it picked up and disposed of by Stericycle Marcia M White Director of Pathology Services Memorial Regional Hospital 3501 Johnson Street Hollywood, Fl 33021 Phone: 954-265-5371 Fax: 954-967-7627 Email: MWhite at mhs.net -----Original Message----- From: Rene J Buesa [mailto:rjbuesa at yahoo.com] Sent: Saturday, June 30, 2018 8:21 AM To: histonet at lists.utsouthwestern.edu; Paula Subject: Re: [Histonet] Disposing Paraffin We incinerated itRen? On ?Friday?, ?June? ?29?, ?2018? ?03?:?49?:?50? ?PM, Paula via Histonet wrote: Hello all.this is an on-going topic with our lab. If anyone can comment about your experience with paraffin waste, I appreciate that in advance. Does your lab or state requirements classify paraffin waste as "hazardous, bio-hazardous, RCRA or other"?? Do you dispose as part of Red Bag trash for autoclave or tissue pathology waste or other hazardous waste for incineration? Paula Lab Manager for Biopath Medical Group in Fountain Valley CA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIFaQ&c=JOaMbd2l0zUJra_1TjFDlA&r=LDt2ZYIvPyjMd5jd_Wmo2w&m=ww_OVwaJOpMAaedehLM4s569VGzTYPbYztTxBeUcTpQ&s=m8G4t7CT5ZXos-1F2SlrayyJ7WDr_rjsH4SugyPYxSE&e= | | Virus-free. www.avast.com | CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. From jqb7 at cdc.gov Mon Jul 23 10:08:54 2018 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Mon, 23 Jul 2018 15:08:54 +0000 Subject: [Histonet] Disposing Paraffin In-Reply-To: <118e8420956f4c29896f597181a807cb@MHSEX05.mhs.net> References: <000901d40fe0$2358a550$6a09eff0$@biopath.org> <827573194.719845.1530361243114@mail.yahoo.com> <118e8420956f4c29896f597181a807cb@MHSEX05.mhs.net> Message-ID: <2debc435084747c887eb149c0dcbd7be@cdc.gov> Hi, We have been instructed to handle the paraffin that does have traces of xylene in it to dispose in the fashion your organization requires. If not it can be placed in the regular trash...for example from your embedding center. Jeanine H. Sanders MS H18-SB Centers for Disease Control and Prevention 1600 Clifton Rd., NE Atlanta, GA 30329 -----Original Message----- From: White, Marcia via Histonet Sent: Monday, July 23, 2018 10:56 AM To: 'Rene J Buesa' ; histonet at lists.utsouthwestern.edu; Paula Subject: Re: [Histonet] Disposing Paraffin We were told that due to the traces of xylene in the paraffin it was considered hazardous so we have it picked up and disposed of by Stericycle Marcia M White Director of Pathology Services Memorial Regional Hospital 3501 Johnson Street Hollywood, Fl 33021 Phone: 954-265-5371 Fax: 954-967-7627 Email: MWhite at mhs.net -----Original Message----- From: Rene J Buesa [mailto:rjbuesa at yahoo.com] Sent: Saturday, June 30, 2018 8:21 AM To: histonet at lists.utsouthwestern.edu; Paula Subject: Re: [Histonet] Disposing Paraffin We incinerated itRen? On ?Friday?, ?June? ?29?, ?2018? ?03?:?49?:?50? ?PM, Paula via Histonet wrote: Hello all.this is an on-going topic with our lab. If anyone can comment about your experience with paraffin waste, I appreciate that in advance. Does your lab or state requirements classify paraffin waste as "hazardous, bio-hazardous, RCRA or other"?? Do you dispose as part of Red Bag trash for autoclave or tissue pathology waste or other hazardous waste for incineration? Paula Lab Manager for Biopath Medical Group in Fountain Valley CA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIFaQ&c=JOaMbd2l0zUJra_1TjFDlA&r=LDt2ZYIvPyjMd5jd_Wmo2w&m=ww_OVwaJOpMAaedehLM4s569VGzTYPbYztTxBeUcTpQ&s=m8G4t7CT5ZXos-1F2SlrayyJ7WDr_rjsH4SugyPYxSE&e= | | Virus-free. www.avast.com | CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From yesyes at comcast.net Mon Jul 23 17:31:48 2018 From: yesyes at comcast.net (Mary Ann) Date: Mon, 23 Jul 2018 18:31:48 -0400 Subject: [Histonet] Positive PAP Message-ID: <46F02EC4-CA5D-4931-9F81-83E7EE0447C6@comcast.net> Help! My pathologist has asked that a positive patient be run down with our PAP stain for QC. Point me to a reference to counter this request. Sent from my iPhone From Samira.Alminawi at sunnybrook.ca Tue Jul 24 08:13:30 2018 From: Samira.Alminawi at sunnybrook.ca (Alminawi, Samira) Date: Tue, 24 Jul 2018 13:13:30 +0000 Subject: [Histonet] BRAF Message-ID: <1e9cd52d184144088209447f84482b6b@SBXNG3.sw.ca> Hi, Anybody using BRAF antibody for melanoma, what is the recommended clone and protocol? samira This e-mail is intended only for the named recipient(s) and may contain confidential, personal and/or health information (information which may be subject to legal restrictions on use, retention and/or disclosure). No waiver of confidence is intended by virtue of communication via the internet. Any review or distribution by anyone other than the person(s) for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and destroy all copies. From criley at dpspa.com Tue Jul 24 09:19:34 2018 From: criley at dpspa.com (Charles Riley) Date: Tue, 24 Jul 2018 10:19:34 -0400 Subject: [Histonet] BRAF In-Reply-To: <1e9cd52d184144088209447f84482b6b@SBXNG3.sw.ca> References: <1e9cd52d184144088209447f84482b6b@SBXNG3.sw.ca> Message-ID: I am interested in the same question. If responding please remember to use the reply all option so everyone can hear the answers. On Tue, Jul 24, 2018 at 9:13 AM, Alminawi, Samira via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi, > Anybody using BRAF antibody for melanoma, what is the recommended clone > and protocol? > samira > > > > This e-mail is intended only for the named recipient(s) and may contain > confidential, personal and/or health information (information which may be > subject to legal restrictions on use, retention and/or disclosure). No > waiver of confidence is intended by virtue of communication via the > internet. Any review or distribution by anyone other than the person(s) > for whom it was originally intended is strictly prohibited. If you have > received this e-mail in error, please contact the sender and destroy all > copies. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From Kelly.Pairan at nationwidechildrens.org Tue Jul 24 09:33:17 2018 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Tue, 24 Jul 2018 14:33:17 +0000 Subject: [Histonet] Choline Transporter Message-ID: Hi Everyone, Does anyone currently use Choline Transporter for Hirschsprung's disease cases on the Leica Bonds? If so, would you be willing to share your protocol? Thanks, Kelly Kelly Pairan, HT (ASCP)CM, QIHC (ASCP) Specialist II-Anatomic Pathology Department of Pathology and Laboratory Medicine Email: kelly.pairan at nationwidechildrens.org ph: 614-722-5414 fx: 614-722-3033 From greg.dobbin at gmail.com Tue Jul 24 13:17:00 2018 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Tue, 24 Jul 2018 15:17:00 -0300 Subject: [Histonet] BRAF Message-ID: Here in Charlottetown we are using V600E (VE-1) clone (Monoclonal) from Spring Diagnostics. We run it on the Bond platforms at 1:100 with ER-2 (high pH) for 30 mins. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From Samira.Alminawi at sunnybrook.ca Tue Jul 24 13:20:46 2018 From: Samira.Alminawi at sunnybrook.ca (Alminawi, Samira) Date: Tue, 24 Jul 2018 18:20:46 +0000 Subject: [Histonet] BRAF In-Reply-To: References: Message-ID: Hi Greg, How is the quality of the staining? Is it working for melanoma? Samira From: Greg Dobbin [mailto:greg.dobbin at gmail.com] Sent: Tuesday, July 24, 2018 2:17 PM To: Alminawi, Samira; histonet at lists.utsouthwestern.edu Subject: RE: BRAF Here in Charlottetown we are using V600E (VE-1) clone (Monoclonal) from Spring Diagnostics. We run it on the Bond platforms at 1:100 with ER-2 (high pH) for 30 mins. Greg -- Greg Dobbin 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 Everything in moderation...even moderation itself! This e-mail is intended only for the named recipient(s) and may contain confidential, personal and/or health information (information which may be subject to legal restrictions on use, retention and/or disclosure). No waiver of confidence is intended by virtue of communication via the internet. Any review or distribution by anyone other than the person(s) for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and destroy all copies. From tbraud at holyredeemer.com Tue Jul 24 13:25:38 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 24 Jul 2018 18:25:38 +0000 Subject: [Histonet] HELP Path requesting +PAP stain control Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF665D9@HRHEX02-HOS.holyredeemer.local> On Date: Mon, 23 Jul 2018 18:31:48 -0400 From: Mary Ann Subject: [Histonet] Positive PAP Mary Ann wrote "Help! My pathologist has asked that a positive patient be run down with our PAP stain for QC. Point me to a reference to counter this request." Hi Mary Ann - First of all, my sympathies. This is the kind of craziness that can give a pathologist a bad name. Secondly, what does he call a patient positive? Positive for what? LOL, JK. In response to your question, here are the ONLY 2 requirements for Cytology stain QC, straight from the latest CAP list. See below. As one can see, nowhere does it require any type of patient control, only a documented assessment of the stain quality, on "actual case material" CAPs words, not mine. Good Luck! Terri __________________________________________________________________ **REVISED** 08/21/2017 CYP.03925 Stain Assessment Phase I Cytology stains are assessed at least annually to ensure their proper storage and acceptable quality. NOTE: Cytology stains undergoing a daily technical quality review are exempt from an annual assessment. Most stains used in the cytology laboratory are not subject to outdating, so that assignment of expiration dates may have no meaning. The acceptable performance of such stains must be confirmed at least annually by technical assessment on actual case material, and as part of the evaluation of cytopathology cases. Where applicable, expiration dates assigned by a manufacturer must be observed. Evidence of Compliance: ? Written procedure for stain assessment AND ? Records of assessment of appropriate quality of each cytology stain in use CYP.04300 Daily QC Phase II Daily QC Phase II There are records of daily review of the technical quality of cytologic preparations by the pathologist or supervisory-level cytotechnologist. NOTE: The technical quality of cytologic preparations must be checked daily (on days processing occurs). This includes checking all stains for predicted staining characteristics each day of use. This check must include all of the types of preparations seen that day such as cytospins, cell blocks, and liquid based preparations. If preparation and staining is performed by a different laboratory, there must be a procedure for the laboratory performing the preparation and staining to verify the acceptability of the quality of preparations and the acceptability of controls (if needed) before transfer. Records of this verification must be readily available to the laboratory performing interpretations. There should also be a mechanism for feedback from the interpreting laboratory to the laboratory that prepared the slides of any issues with the preparations. _________________________________________________________________ Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From greg.dobbin at gmail.com Tue Jul 24 13:40:12 2018 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Tue, 24 Jul 2018 15:40:12 -0300 Subject: [Histonet] BRAF In-Reply-To: References: Message-ID: Yes, it works. Very well actually. And we have done well on both a cIQc and a CAP survey. Greg On Tue, Jul 24, 2018 at 3:20 PM Alminawi, Samira < Samira.Alminawi at sunnybrook.ca> wrote: > Hi Greg, > > How is the quality of the staining? Is it working for melanoma? > > Samira > > > > *From:* Greg Dobbin [mailto:greg.dobbin at gmail.com] > *Sent:* Tuesday, July 24, 2018 2:17 PM > *To:* Alminawi, Samira; histonet at lists.utsouthwestern.edu > *Subject:* RE: BRAF > > > > Here in Charlottetown we are using V600E (VE-1) clone (Monoclonal) from > Spring Diagnostics. We run it on the Bond platforms at 1:100 with ER-2 > (high pH) for 30 mins. > > Greg > > > > -- > > *Greg Dobbin* > 1205 Pleasant Grove Rd > RR#2 York, > PE C0A 1P0 > > > > *Everything in moderation...even moderation itself!* > > *This e-mail is intended only for the named recipient(s) and may contain > confidential, personal and/or health information (information which may be > subject to legal restrictions on use, retention and/or disclosure). No > waiver of confidence is intended by virtue of communication via the > internet. Any review or distribution by anyone other than the person(s) > for whom it was originally intended is strictly prohibited. If you have > received this e-mail in error, please contact the sender and destroy all > copies.* > > > -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From dr at personifysearch.com Tue Jul 24 15:32:37 2018 From: dr at personifysearch.com (Danielle Robinson) Date: Tue, 24 Jul 2018 16:32:37 -0400 Subject: [Histonet] Job Opportunity - Histology Manager - Northern Ohio Message-ID: Hello Everyone, We have a great opportunity with a world leader in pre-clinical research. If you are interested or know anyone who may be a good fit, please feel free to email me directly (dr at personifysearch.com). I am including a position description below. Sorry about the formatting! Job Summary Designs and/or executes scientific testing strategies and studies. Leads assay development, assay validation or study conduct, or is involved in preparation of material (e.g. protein, nucleic acid, cells, etc.). Reviews and interprets study data, communicates results to clients and writes final reports. Serves as Project Scientist, Principal Investigator, Contributing Scientist, Project Leader or Study Director, as applicable. Ensures compliance with protocols and all applicable SOPs. Troubleshoots and resolves assay or technical issues in the laboratory when scientific expertise is needed. May introduce new technologies or introduce improvements in existing technologies. Provides advisory functions to clients designing a program or experiment, dealing with specific dataset interpretation, or when appropriate answering questions from regulatory authorities. *Essential Duties and Responsibilities:* Manage activities of the laboratory to include administrative tasks, meeting finanacial objectives, meeting study milestones, and training, coordinating activities between the laboratory and other groups within and external to the pathology department. Responsible for coordination, planning and scheduling of laboratory projects. Oversee departmental procedures for sample receipt, handling, tracking, transfer, shipping, etc. Manage activities of assigned group(s) to ensure effective performance of function. Interview and participate in the selection of qualified exempt- and non-exempt level departmental personnel. Recommend, review and approve personnel actions, including hiring, promotions and raises. Partner with Human Resources in the handling of disciplinary issues. Monitor performance of direct reports. Provide regular coaching and counseling. Prepare and deliver salary and performance reviews. *Qualifications:* * Education:* Bachelor's degree or equivalent in a biological science preferred. * Experience:* 5 years of pathology laboratory experience preferred. Previous experience supervising and/or managing a laboratory under GLP regulations is required. * Certification:* HT (ASCP) or HLT (ASCP) required *Physical Demands:* Employees must be able to lift, move, manipulate, and/or hold heavy objects up to and including 50 pounds; this includes work materials, equipment, and/or animals. Must be able to perform laboratory procedures, which require, talking, hearing, standing or sitting for long periods of time, entering data into a computer, using appropriate instruments, reaching with hands and arms, working in narrow spaces, and wearing safety euqipment (PPE) according to OSHA regulations and company standards. Specific vision abilities required by this job include close vision, color vision, depth perception and the ability to adjust focus. Thank you! Danielle -- Danielle Robinson Sr. Team Lead, Talent Acquisition *Personify* 416 S. Dawson Street Raleigh, NC 27601 (Direct) 919.460.8726 www.personifysearch.com -- CONFIDENTIALITY NOTICE: ?The contents of this email message and any attachments are intended solely for the addressee(s) and may contain confidential and/or privileged information and may be legally protected from disclosure. ?If you are not the intended recipient of this message or their agent, or if this message has been addressed to you in error, please immediately alert the sender by reply email and then delete this message and any attachments. If you are not the intended recipient, you are hereby notified that any use, dissemination, copying, or storage of this message or its attachments is strictly prohibited. From Samira.Alminawi at sunnybrook.ca Wed Jul 25 08:08:22 2018 From: Samira.Alminawi at sunnybrook.ca (Alminawi, Samira) Date: Wed, 25 Jul 2018 13:08:22 +0000 Subject: [Histonet] BRAF In-Reply-To: References: Message-ID: Hi, Thank you Greg. Samira From: Greg Dobbin [mailto:greg.dobbin at gmail.com] Sent: Tuesday, July 24, 2018 2:17 PM To: Alminawi, Samira; histonet at lists.utsouthwestern.edu Subject: RE: BRAF Here in Charlottetown we are using V600E (VE-1) clone (Monoclonal) from Spring Diagnostics. We run it on the Bond platforms at 1:100 with ER-2 (high pH) for 30 mins. Greg -- Greg Dobbin 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 Everything in moderation...even moderation itself! This e-mail is intended only for the named recipient(s) and may contain confidential, personal and/or health information (information which may be subject to legal restrictions on use, retention and/or disclosure). No waiver of confidence is intended by virtue of communication via the internet. Any review or distribution by anyone other than the person(s) for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and destroy all copies. From relia1 at earthlink.net Wed Jul 25 08:59:25 2018 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 25 Jul 2018 09:59:25 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin I hope you are enjoying an Amazing Summer!! Message-ID: <001001d4241f$b47b42b0$1d71c810$@earthlink.net> Hello Histonetters, I hope you are having a great week. Can you believe we are 2/3?s of the way through the summer? NSH/SC in St. Louis is right around the corner!! Time sure flies when you?re having fun!! I hope your summer has been as amazing as mine! I wanted to send a quick note to tell you about the positions that I am working on and am most excited about.? Why am I excited about these positions? Because each of these clients was asked if I had someone for you today would you be ready to interview and hire Every One of these clients said YES!!! Here are the Super Sizzlin Hot Spotlight Opportunities! Atlanta, GA - HT/HTL?DAYS!! ? Gross, cut, embed, specials, IHC! North Carolina - HT/HTL?DAYS!! - cut, embed, specials and IHC! Kansas City, MO HT/HTL DAYS!! ? Derm and Mohs! Here are more terrific opportunities that you may have missed! Ridgecrest, CA HT/HTL-DAYS! Gross, cut, embed, specials, IHC Toledo, OH HT/HTL Days!! Supervisor or Lead Tech!! Austin, TX HT/HTL Days!! cut, embed, specials and IHC! All of these positions are full time and permanent!! My clients offer excellent compensation, benefits and in most cases either relocation or a sign-on bonus. Histonetters, If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I place someone you refer You will earn a referral fee. If you are interested in any of these opportunities CALL /TEXT MY CELL ASAP!!! At 407-353-5070. I can also be reached toll free at the office at 866-607-3542 or at relia1 at earthlink.net to set up a time to talk at your convenience! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From brannon at alliedsearchpartners.com Wed Jul 25 15:05:53 2018 From: brannon at alliedsearchpartners.com (Brannon Owens) Date: Wed, 25 Jul 2018 20:05:53 +0000 Subject: [Histonet] Histology Supervisor job opening in Tampa, FL Message-ID: Position Title: Histology Supervisor Shift: M-F day shift; 8am - 4:30pm Location: Tampa, FL Job Responsibilities The Histology Supervisor oversees the daily operations of the Histology laboratory section. This oversight includes technical supervision of all departmental processes, testing procedures, as well as management and supervision of personnel. Additional duties of the Histology Supervisor: * Responsible for the completion of performance appraisals, competency, and addressing any performance or conduct related issues with staff. * Ensures compliance with all regulatory and accreditation requirements pertaining to the processing of specimens and the performance of all histology procedures. * Under general supervision of the Medical Director, ensures quality, problem resolution, and validation/implementation of new tests and technologies. * Budget review and preparation, and inventory management. * Histology Supervisor may also perform the duties of a technologist or assistant as workload requires. Histology Supervisor - Healthcare Sciences (Histology Technician / Technologist) Job Requirements Successful candidates for the Histology Supervisor will have certification by American Society for Clinical Pathology (ASCP) as a Histotechnician (HT) or Histotechnologist (HTL). Additional requirements of the Histology Supervisor role include: * Bachelor's degree in Histotechnology or related Life Sciences/Health Sciences degree. * Minimum 5 years of experience working as a Histotechnologist or Histotechnician * Ability to problem solve and troubleshoot technical problems, adapt positively to change, and supervise a diverse team of personnel. * Relevant Supervisory experience preferred * FL Supervisor license Histology Supervisor - Healthcare Sciences (Histology Technician / Technologist) To apply: Please send resume to brannon at alliedsearchpartners.com or fax to 888 388 7572. 2018 Forbes 100 Best Professional Recruitment Firms in American and the only Laboratory Staffing Specific Firm on List! https://www.forbes.com/best-professional-recruiting-firms/list/ Brannon Owens VP/Director of Recruitment Allied Search Partners LinkedIn: https://www.linkedin.com/in/jbrannonowens/ http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 Direct Line: 407.413.9421 F: 888.388.7572 This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. From houv2 at uw.edu Thu Jul 26 13:19:22 2018 From: houv2 at uw.edu (Vivian Hou) Date: Thu, 26 Jul 2018 11:19:22 -0700 Subject: [Histonet] Coronary or Muscular Artery Histology Textbooks Message-ID: Dear all, I am searching for textbooks (not journal papers) focused more towards coronary or muscular artery histology (or just vascular anatomy overall), any suggestions you may have will be greatly appreciated! Thank you all for your time, Vivian -- ----------------------------------------------------------------------------------------- V Hou Research Scientist | Engineer III Center for CardioVascular Innovation e: houv2 at uw.edu | p: 206-221-3053 From wdesalvo.cac at outlook.com Thu Jul 26 14:26:51 2018 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Thu, 26 Jul 2018 19:26:51 +0000 Subject: [Histonet] Coronary or Muscular Artery Histology Textbooks In-Reply-To: References: Message-ID: Stevens and Lowe, Histology, 4th edition, 2015. Good human anatomy and descriptive William DeSalvo ________________________________ From: Vivian Hou via Histonet Sent: Thursday, July 26, 2018 11:40 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Coronary or Muscular Artery Histology Textbooks Dear all, I am searching for textbooks (not journal papers) focused more towards coronary or muscular artery histology (or just vascular anatomy overall), any suggestions you may have will be greatly appreciated! Thank you all for your time, Vivian -- ----------------------------------------------------------------------------------------- V Hou Research Scientist | Engineer III Center for CardioVascular Innovation e: houv2 at uw.edu | p: 206-221-3053 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam02.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7C%7C49d13103220a402524fc08d5f3274abc%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636682272432887926&sdata=fXkCBKL3yIxVBIyC5eO0pqcm8K5%2FPeiPJ82ySYQuVls%3D&reserved=0 From koellingr at comcast.net Thu Jul 26 15:09:20 2018 From: koellingr at comcast.net (RAY KOELLING) Date: Thu, 26 Jul 2018 13:09:20 -0700 (PDT) Subject: [Histonet] Coronary or Muscular Artery Histology Textbooks In-Reply-To: References: Message-ID: <636990346.714325.1532635760194@connect.xfinity.com> Vivian, although I'm not in that building anymore I believe that CCVI is in Health Sciences Building. If so, the UW School of Medicine, Dept of Cardiology along with the Health Sciences Library-where I spent about 6 years- is within that building so a brief walk and has more basic to advanced textbooks of coronary or muscular artery histology than you could ever read in a lifetime. Ray, UW School of Medicine-Spokane WWAMI site > On July 26, 2018 at 11:19 AM Vivian Hou via Histonet wrote: > > > Dear all, > > I am searching for textbooks (not journal papers) focused more towards > coronary or muscular artery histology (or just vascular anatomy overall), > any suggestions you may have will be greatly appreciated! > > Thank you all for your time, > Vivian > > -- > ----------------------------------------------------------------------------------------- > V Hou > Research Scientist | Engineer III > Center for CardioVascular Innovation > e: houv2 at uw.edu | p: 206-221-3053 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From houv2 at uw.edu Thu Jul 26 15:20:54 2018 From: houv2 at uw.edu (Vivian Hou) Date: Thu, 26 Jul 2018 13:20:54 -0700 Subject: [Histonet] Coronary or Muscular Artery Histology Textbooks In-Reply-To: <636990346.714325.1532635760194@connect.xfinity.com> References: <636990346.714325.1532635760194@connect.xfinity.com> Message-ID: Hi Ray, Thank you! I have been looking at the collection slowly and I am hoping to catalyze my sorting process by asking for recommendations of specific titles or references, if you have any I would be very grateful! Thank you, Vivian On Thu, Jul 26, 2018 at 1:09 PM, RAY KOELLING wrote: > Vivian, although I'm not in that building anymore I believe that CCVI is > in Health Sciences Building. If so, the UW School of Medicine, Dept of > Cardiology along with the Health Sciences Library-where I spent about 6 > years- is within that building so a brief walk and has more basic to > advanced textbooks of coronary or muscular artery histology than you could > ever read in a lifetime. > > Ray, UW School of Medicine-Spokane WWAMI site > > > On July 26, 2018 at 11:19 AM Vivian Hou via Histonet utsouthwestern.edu> wrote: > > > > > > Dear all, > > > > I am searching for textbooks (not journal papers) focused more towards > > coronary or muscular artery histology (or just vascular anatomy overall), > > any suggestions you may have will be greatly appreciated! > > > > Thank you all for your time, > > Vivian > > > > -- > > ------------------------------------------------------------ > ----------------------------- > > V Hou > > Research Scientist | Engineer III > > Center for CardioVascular Innovation > > e: houv2 at uw.edu | p: 206-221-3053 > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- ----------------------------------------------------------------------------------------- V Hou Research Scientist | Engineer III Center for CardioVascular Innovation e: houv2 at uw.edu | p: 206-221-3053 From Blanca.Lopez at UTSouthwestern.edu Fri Jul 27 10:11:02 2018 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Fri, 27 Jul 2018 15:11:02 +0000 Subject: [Histonet] formalin vs alcohol storage Message-ID: Morning Histonnetes: Can somebody give your opinion on how to storage alcohol and formalin? Is formalin consider corrosive that can't be storage together with alcohol? What is your opinion? Thanks for your help... Blanca Lopez HT (ASCP) Histotechnologist UT Southwestern Medical Center Harold C. Simmons Comprehensive Cancer Center Histology Lab K1-210 214-648-7598 blanca.lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From tmcampbe at fmh.org Fri Jul 27 10:16:26 2018 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Fri, 27 Jul 2018 15:16:26 +0000 Subject: [Histonet] Paperwork Message-ID: Hi everyone, I have 2 questions: 1. Could someone please share some ways to keep track of the control that goes with the slides that it was used for? So I am a small GI lab and there is a pathologist here a couple days a week. We do trichrome on Microscopic colitis cases and so I have been batching the trichromes because it's a long stain to do by hand and it's a lot easier to do it that way. But my slides are automatically printed for me and they have the date on them that the specimen was entered into the system. I cut the slide but then hold it until I am ready to do the stain. I put a date on the trichrome control slide but it of course does not match the date on the patient slides because they have been held for a few days. Is this something I even need to worry about? So far I have just been writing down the date that I stain the patient slide on a log sheet but I am trying to minimize the amount of paperwork/manual logging. 1. We recently got a accessioning system and I can now pull the number of blocks and stains done each day. Do I need to still keep writing down in my log sheet the number of blocks and stains? Do I need to print out the report that has the numbers and file it or since I have the ability to pull it from the system, I don't need to have physical logs. I am just trying to minimize as much manual logging and paperwork as possible! Thanks in Advance!!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 From wdesalvo.cac at outlook.com Fri Jul 27 12:20:20 2018 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Fri, 27 Jul 2018 17:20:20 +0000 Subject: [Histonet] Paperwork In-Reply-To: References: Message-ID: I have a few suggestions: Batch control - you do need to continue documentation of which cases/slides corresponds to the one control and be able to provide for inspection or re-review of a case. I suggest you consider taking pre-cut slide, add a new cut control section (1 per case if there are multiple slides) before staining. This conserves control tissue and removes some of the logistics of locating and matching batch control. I believe this will be a quality improvement without high cost. Accessioning/ LIS - Ditch the log sheets and do not print, unless there is a specific need. All of your manual tracking process is now electronic. Do update all SOP?s and note date of change in process. William William DeSalvo ________________________________ From: Campbell, Tasha M. via Histonet Sent: Friday, July 27, 2018 8:41 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Paperwork Hi everyone, I have 2 questions: 1. Could someone please share some ways to keep track of the control that goes with the slides that it was used for? So I am a small GI lab and there is a pathologist here a couple days a week. We do trichrome on Microscopic colitis cases and so I have been batching the trichromes because it's a long stain to do by hand and it's a lot easier to do it that way. But my slides are automatically printed for me and they have the date on them that the specimen was entered into the system. I cut the slide but then hold it until I am ready to do the stain. I put a date on the trichrome control slide but it of course does not match the date on the patient slides because they have been held for a few days. Is this something I even need to worry about? So far I have just been writing down the date that I stain the patient slide on a log sheet but I am trying to minimize the amount of paperwork/manual logging. 1. We recently got a accessioning system and I can now pull the number of blocks and stains done each day. Do I need to still keep writing down in my log sheet the number of blocks and stains? Do I need to print out the report that has the numbers and file it or since I have the ability to pull it from the system, I don't need to have physical logs. I am just trying to minimize as much manual logging and paperwork as possible! Thanks in Advance!!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7C%7C50f4f767808d48b6bc9708d5f3d75d0b%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636683028652780421&sdata=X1fvAOeKBub4UgTjZcdLcjwC7TiZ9xN5Ic7zDifXcjY%3D&reserved=0 From tmcampbe at fmh.org Fri Jul 27 13:00:35 2018 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Fri, 27 Jul 2018 18:00:35 +0000 Subject: [Histonet] Paperwork In-Reply-To: References: Message-ID: I am a little confused about your batch control explanation. Do you mean to put a piece of control tissue on every case slide? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 ________________________________ From: WILLIAM DESALVO [mailto:wdesalvo.cac at outlook.com] Sent: Friday, July 27, 2018 1:20 PM To: Campbell, Tasha M.; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Paperwork I have a few suggestions: Batch control - you do need to continue documentation of which cases/slides corresponds to the one control and be able to provide for inspection or re-review of a case. I suggest you consider taking pre-cut slide, add a new cut control section (1 per case if there are multiple slides) before staining. This conserves control tissue and removes some of the logistics of locating and matching batch control. I believe this will be a quality improvement without high cost. Accessioning/ LIS - Ditch the log sheets and do not print, unless there is a specific need. All of your manual tracking process is now electronic. Do update all SOP's and note date of change in process. William William DeSalvo ________________________________ From: Campbell, Tasha M. via Histonet Sent: Friday, July 27, 2018 8:41 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Paperwork Hi everyone, I have 2 questions: 1. Could someone please share some ways to keep track of the control that goes with the slides that it was used for? So I am a small GI lab and there is a pathologist here a couple days a week. We do trichrome on Microscopic colitis cases and so I have been batching the trichromes because it's a long stain to do by hand and it's a lot easier to do it that way. But my slides are automatically printed for me and they have the date on them that the specimen was entered into the system. I cut the slide but then hold it until I am ready to do the stain. I put a date on the trichrome control slide but it of course does not match the date on the patient slides because they have been held for a few days. Is this something I even need to worry about? So far I have just been writing down the date that I stain the patient slide on a log sheet but I am trying to minimize the amount of paperwork/manual logging. 1. We recently got a accessioning system and I can now pull the number of blocks and stains done each day. Do I need to still keep writing down in my log sheet the number of blocks and stains? Do I need to print out the report that has the numbers and file it or since I have the ability to pull it from the system, I don't need to have physical logs. I am just trying to minimize as much manual logging and paperwork as possible! Thanks in Advance!!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7C%7C50f4f767808d48b6bc9708d5f3d75d0b%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636683028652780421&sdata=X1fvAOeKBub4UgTjZcdLcjwC7TiZ9xN5Ic7zDifXcjY%3D&reserved=0 From tbraud at holyredeemer.com Fri Jul 27 13:18:02 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 27 Jul 2018 18:18:02 +0000 Subject: [Histonet] Paperwork / tracking batch controls Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF67067@HRHEX02-HOS.holyredeemer.local> There is an easy method to track batch controls using a blank slide. For each case stained, file a blank slide that has been labeled with the actual case number, the stain, and also the date of the control slide. Easy peasy. I published this method in Histologic in Aug. 1995 and it has stood the test of CAP and time. As far as recording blocks and slides, if your new computer software system keeps those records and they can be retrieved easily, then you don't need to keep paper anymore. Happy Histo-ing! Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 6. Paperwork (Campbell, Tasha M.) Message: 6 Date: Fri, 27 Jul 2018 15:16:26 +0000 From: "Campbell, Tasha M." Hi everyone, I have 2 questions: 1. Could someone please share some ways to keep track of the control that goes with the slides that it was used for? So I am a small GI lab and there is a pathologist here a couple days a week. We do trichrome on Microscopic colitis cases and so I have been batching the trichromes because it's a long stain to do by hand and it's a lot easier to do it that way. But my slides are automatically printed for me and they have the date on them that the specimen was entered into the system. I cut the slide but then hold it until I am ready to do the stain. I put a date on the trichrome control slide but it of course does not match the date on the patient slides because they have been held for a few days. Is this something I even need to worry about? So far I have just been writing down the date that I stain the patient slide on a log sheet but I am trying to minimize the amount of paperwork/manual logging. 1. We recently got a accessioning system and I can now pull the number of blocks and stains done each day. Do I need to still keep writing down in my log sheet the number of blocks and stains? Do I need to print out the report that has the numbers and file it or since I have the ability to pull it from the system, I don't need to have physical logs. I am just trying to minimize as much manual logging and paperwork as possible! Thanks in Advance!!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 176, Issue 17 ***************************************** From wdesalvo.cac at outlook.com Fri Jul 27 14:06:14 2018 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Fri, 27 Jul 2018 19:06:14 +0000 Subject: [Histonet] Paperwork In-Reply-To: References: , Message-ID: I am not a a big supporter of batch controls and managing that process. Place a control section above patient sample, choosing 1 slide/case, prior to moving slides to stain. Control always gets to pathologist that is reading patient slides, assuming patient slides always get to pathologist and control is filed with the case. Think through what are the opportunities for error and develop a change to either eliminate or reduce the opportunity. There are multiple solutions to your problem and you will need to decide what process change gives you the results you desire. There should be multiple responses to facilitate your change. William William DeSalvo ________________________________ From: Campbell, Tasha M. Sent: Friday, July 27, 2018 11:00 AM To: WILLIAM DESALVO; histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] Paperwork I am a little confused about your batch control explanation. Do you mean to put a piece of control tissue on every case slide? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 ________________________________ From: WILLIAM DESALVO [mailto:wdesalvo.cac at outlook.com] Sent: Friday, July 27, 2018 1:20 PM To: Campbell, Tasha M.; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Paperwork I have a few suggestions: Batch control - you do need to continue documentation of which cases/slides corresponds to the one control and be able to provide for inspection or re-review of a case. I suggest you consider taking pre-cut slide, add a new cut control section (1 per case if there are multiple slides) before staining. This conserves control tissue and removes some of the logistics of locating and matching batch control. I believe this will be a quality improvement without high cost. Accessioning/ LIS - Ditch the log sheets and do not print, unless there is a specific need. All of your manual tracking process is now electronic. Do update all SOP?s and note date of change in process. William William DeSalvo ________________________________ From: Campbell, Tasha M. via Histonet Sent: Friday, July 27, 2018 8:41 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Paperwork Hi everyone, I have 2 questions: 1. Could someone please share some ways to keep track of the control that goes with the slides that it was used for? So I am a small GI lab and there is a pathologist here a couple days a week. We do trichrome on Microscopic colitis cases and so I have been batching the trichromes because it's a long stain to do by hand and it's a lot easier to do it that way. But my slides are automatically printed for me and they have the date on them that the specimen was entered into the system. I cut the slide but then hold it until I am ready to do the stain. I put a date on the trichrome control slide but it of course does not match the date on the patient slides because they have been held for a few days. Is this something I even need to worry about? So far I have just been writing down the date that I stain the patient slide on a log sheet but I am trying to minimize the amount of paperwork/manual logging. 1. We recently got a accessioning system and I can now pull the number of blocks and stains done each day. Do I need to still keep writing down in my log sheet the number of blocks and stains? Do I need to print out the report that has the numbers and file it or since I have the ability to pull it from the system, I don't need to have physical logs. I am just trying to minimize as much manual logging and paperwork as possible! Thanks in Advance!!! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7C%7C50f4f767808d48b6bc9708d5f3d75d0b%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636683028652780421&sdata=X1fvAOeKBub4UgTjZcdLcjwC7TiZ9xN5Ic7zDifXcjY%3D&reserved=0 From Muhammad.Azam2 at va.gov Mon Jul 30 12:44:06 2018 From: Muhammad.Azam2 at va.gov (Azam, Muhammad) Date: Mon, 30 Jul 2018 17:44:06 +0000 Subject: [Histonet] Davidson Message-ID: Hi; I am wondering what is the optimal time for Davidson's fixative (range). This is in regards to a sectioned lymph node. Thanks; Muhammad Azam, MD Staff Pathologist VHAMOU From wdesalvo.cac at outlook.com Mon Jul 30 13:40:49 2018 From: wdesalvo.cac at outlook.com (WILLIAM DESALVO) Date: Mon, 30 Jul 2018 18:40:49 +0000 Subject: [Histonet] Davidson In-Reply-To: References: Message-ID: Davidson?s fixative is suggested to for use to fix in up to 24 hours. The small nodes should be at least halved and larger nodes cut no more than 2-3 mm thick. There are a few papers that state ?small biopsies can be fixed rapidly?. I suggest < 6 hours for small biopsies. I would do, minimally, a quick verification study on multiple tissue samples before risking diagnostic samples. Hope that helps. William William DeSalvo ________________________________ From: Azam, Muhammad via Histonet Sent: Monday, July 30, 2018 10:55 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Davidson Hi; I am wondering what is the optimal time for Davidson's fixative (range). This is in regards to a sectioned lymph node. Thanks; Muhammad Azam, MD Staff Pathologist VHAMOU _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7C%7Ccc9e5c75a99c490571f608d5f645a93f%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C636685701408677759&sdata=KkIsiKg5Gb4Z95VrNryIIb%2B232gTbahSY%2Bt5kMYbrD0%3D&reserved=0 From Scott.Lindrud at carrishealth.com Tue Jul 31 07:33:10 2018 From: Scott.Lindrud at carrishealth.com (Lindrud, Scott) Date: Tue, 31 Jul 2018 12:33:10 +0000 Subject: [Histonet] Fat Aspirates for Amyloidosis Message-ID: Hi Histonetters, Would anyone be willing to share how they process a Fat Pad aspirate for Amyloidosis? Our method does not provide a very good result and we're looking to improve on how we do this. We collect direct aspirates using an 18 gauge needle and make air dried smears and stain with Congo Red. We also get additional passes and place directly in 10% NBF and will try to make a cell block using histogel out of them. Problem is fat floats and it is difficult to get a good cell block made. I would think spinning the 10%NBF and filtering out the fat and placing directly into a biopsy bag or wrapping in paper would be the way to go. After you process the block, I'm guessing all the lipid will process out and there would only be cell membranes from the fibroadipose tissue left over. Wouldn't you need a significant amount of fat to process to have anything to embed? I've seen suggestions on other websites suggesting 35-50 mg of fat. Maybe we need to go to a larger needle, maybe 16 gauge? Any ideas would be greatly appreciated! Scott Scott A. Lindrud, MLS(ASCP) CM CT CM Histopathology Technical Specialist/Cytotechnologist CarrisHealth Rice Memorial Hospital 301 Becker Ave SW | Willmar | MN | 56201 scott.lindrud at carrishealth.com 320-231-4406/4520 320-231-4503 (fax) Carris Health is a new entity created to deliver health care to West Central and Southwest Minnesota. Carris Health is a partnership between CentraCare Health, Rice Memorial Hospital and ACMC Health. www.carrishealth.com Confidentiality Notice: This e-mail and any attachment may contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. 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