From litepath2000 at yahoo.com Thu Feb 1 07:46:01 2018 From: litepath2000 at yahoo.com (LitePath) Date: Thu, 1 Feb 2018 13:46:01 +0000 (UTC) Subject: [Histonet] Making Bulk Reagents for Ventana In-Reply-To: References: Message-ID: <1563595356.1255296.1517492761325@mail.yahoo.com> Hi JAmesCurious, did you get any response to this???ThanksLuis NYULMCNY, NY On Thursday, January 25, 2018, 12:10:04 PM EST, Nutter, James E via Histonet wrote: Hi Everyone, Has anyone or does anyone make the bulk reagents for the Ventana Ultras?? (LCS, SSC, Reaction Buffer, CC1, CC2) If so: How to turn off the tracking system for bulk reagents? Formulations? Success? Thank you all, James Nutter Chantilly, VA ______________________________________________________________________ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ryan.Roy at va.gov Fri Feb 2 10:46:18 2018 From: Ryan.Roy at va.gov (Roy, Ryan) Date: Fri, 2 Feb 2018 16:46:18 +0000 Subject: [Histonet] Aperio CS2 Message-ID: Anyone out there working with Aperio CS2? How does it function for you? We want to use it for consultations. Ryan From lisab at hollandhospital.org Fri Feb 2 12:35:33 2018 From: lisab at hollandhospital.org (Lisa Brenner) Date: Fri, 02 Feb 2018 13:35:33 -0500 Subject: [Histonet] tissue processor Message-ID: <5A7469250200003C000644B4@nvgw04p> Hello, We are in the market for a new vacuum infiltrating tissue processor. Our old VIP has been a work horse but needs replacing. What is everyone using? What works well? What's new? Lisa Brenner Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. From bcooper at chla.usc.edu Fri Feb 2 12:43:13 2018 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Fri, 2 Feb 2018 18:43:13 +0000 Subject: [Histonet] tissue processor In-Reply-To: <5A7469250200003C000644B4@nvgw04p> References: <5A7469250200003C000644B4@nvgw04p> Message-ID: We absolutely love our Leica Peloris II. Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper at chla.usc.edu -----Original Message----- From: Lisa Brenner via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, February 02, 2018 10:36 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] tissue processor (EXTERNAL EMAIL) Hello, We are in the market for a new vacuum infiltrating tissue processor. Our old VIP has been a work horse but needs replacing. What is everyone using? What works well? What's new? Lisa Brenner Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From tgenade at gmail.com Fri Feb 2 15:34:55 2018 From: tgenade at gmail.com (Tyrone Genade) Date: Fri, 2 Feb 2018 15:34:55 -0600 Subject: [Histonet] Wright-Giemsa for sections? Message-ID: Hello, Can the Wright-Giemsa stain be used on fixed, paraffin embedded sections? Does anyone have a protocol? I want to examine hematopoietic tissue of fish, i.e. the head kidney. No smears or imprint possible. I would like to use Wrights so I can use the same stain for blood smears. Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. *Please note: I don't usually check my email over the weekend. * From llewllew at shaw.ca Fri Feb 2 18:21:17 2018 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Fri, 2 Feb 2018 16:21:17 -0800 Subject: [Histonet] Wright-Giemsa for sections? In-Reply-To: References: Message-ID: <7527ac23-1ac8-1e27-baf2-3d4671a33b10@shaw.ca> Go to http://stainsfile.info/StainsFile/stain/oversight/romanowsky.htm Bryan Llewellyn Tyrone Genade via Histonet wrote: > Hello, > > Can the Wright-Giemsa stain be used on fixed, paraffin embedded sections? > Does anyone have a protocol? > > I want to examine hematopoietic tissue of fish, i.e. the head kidney. No > smears or imprint possible. I would like to use Wrights so I can use the > same stain for blood smears. > > Thanks > From rsrichmond at gmail.com Sat Feb 3 12:50:11 2018 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 3 Feb 2018 13:50:11 -0500 Subject: [Histonet] Wright-Giemsa for sections? Message-ID: Tyrone Genade in Orange City, Iowa asks: >>Can the Wright-Giemsa stain be used on fixed, paraffin embedded sections? Does anyone have a protocol? I want to examine hematopoietic tissue of fish, i.e. the head kidney. No smears or imprint possible. I would like to use Wrights so I can use the same stain for blood smears.<< Bryan Llewellyn refers you to http://stainsfile.info/StainsFile/stain/oversight/romanowsky.htm That method prescribes neutral buffered formalin fixation. Us old-timers preferred Zenker fixation, which you can't do today on account of the mercury in it. You may have to try different fixatives. The differentiation under microscopic control is really necessary - when I was a resident at Cornell Medical Center in NYC fifty years ago, the histotechs would push the residents off the double-headed microscopes when they sat down to differentiate the bone marrow biopsy section Giemsa stains. The old-timers used a 10% solution of colophonium rosin (almost pure abietic acid) in alcohol, instead of acetic acid, to differentiate the stain. You buy Giemsa or Wright or Romanovsky stain already dissolved in absolute alcohol. You have to be a fanatic about not contaminating your stock bottle with the tiniest trace of water. Bob Richmond Samurai Pathologist Maryville TN From rjbuesa at yahoo.com Sat Feb 3 16:00:46 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Sat, 3 Feb 2018 22:00:46 +0000 (UTC) Subject: [Histonet] Wright-Giemsa for sections? In-Reply-To: References: Message-ID: <1848539195.2683845.1517695246444@mail.yahoo.com> Tyrone:Under separate cover I am sending a procedure I published about this method.Ren? On Friday, February 2, 2018 4:44 PM, Tyrone Genade via Histonet wrote: Hello, Can the Wright-Giemsa stain be used on fixed, paraffin embedded sections? Does anyone have a protocol? I want to examine hematopoietic tissue of fish, i.e. the head kidney. No smears or imprint possible. I would like to use Wrights so I can use the same stain for blood smears. Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. *Please note: I don't usually check my email over the weekend. * _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet | | Virus-free. www.avast.com | From drmoses111 at comcast.net Sat Feb 3 17:57:10 2018 From: drmoses111 at comcast.net (=?utf-8?B?ZHJtb3NlczExMUBjb21jYXN0Lm5ldA==?=) Date: Sat, 3 Feb 2018 23:57:10 GMT Subject: [Histonet] Second shift job in South Jersey Message-ID: <000f4242.050d2e81024e706d@comcast.net> Second shift Histology position opening available, Virtua Health, Voorhees, New Jersey. Experience preferred with HT certification.Please contact: Roxann Thompson? ? ? ? ? ? ? ? ? ? ? ? ? ? ?rthompson at virtua.org? ? ? ? ? ? ? ? ? ? ? ? ? ? ?856-247-3144?? ? ? ? ? ? ? ? ? ? ? ? ? ?? From histo at pathlab.us Mon Feb 5 11:48:54 2018 From: histo at pathlab.us (Histology) Date: Mon, 5 Feb 2018 17:48:54 +0000 Subject: [Histonet] Fridge and freezer temps Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD8206DAD83A@vdc01.domain.local> Hi All, I'm trying to figure out how everyone keeps their refrigerator and freezer temperatures in a certain range. I have 2 house refrigerators with freezers on top. We have the min/max thermometers. I cannot get the freezer to stay between 0 and -8 degrees without affecting the fridge temp. Are other labs using better quality equipment? What are the ranges you are using for both fridge and freezer. Our is 2 to 8 for fridge and 0 and -8 for freezer. Thanks to all, Mehndi Helgren From tbraud at holyredeemer.com Mon Feb 5 12:36:52 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 5 Feb 2018 18:36:52 +0000 Subject: [Histonet] Tissue Processor Message-ID: <48E053DDF6CE074DB6A7414BA05403F84CEACA11@HRHEX03-HOS.holyredeemer.local> After 40 years of using primarily Miles/Tissue Tek, we recently purchased 2 Leica ASP 6025. With the exception of a small glitch with faulty sensors, and a learning curve to get used to them, I would highly recommend them. We had replaced an ancient K series(with the magnet) and a VIP 5. The quality and speed of the processing from the Leica's is incredible. We had great tech support in setting up and validating processing protocols. We ended up using several of their pre-set protocols which worked great, especially for the processing of large tissues. I was expecting better processing than our ancient Tissue Tek, but the idea that it outperformed the VIP 5 surprised me. Reagents are a snap to exchange and the software/screen is very user friendly. I hope this helps, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. tissue processor (Lisa Brenner) ---------------------------------------------------------------------- Message: 1 From: "Lisa Brenner" Subject: [Histonet] tissue processor Hello, We are in the market for a new vacuum infiltrating tissue processor. Our old VIP has been a work horse but needs replacing. What is everyone using? What works well? What's new? Lisa Brenner ****** From tgenade at gmail.com Mon Feb 5 13:06:21 2018 From: tgenade at gmail.com (Tyrone Genade) Date: Mon, 5 Feb 2018 13:06:21 -0600 Subject: [Histonet] Histonet Digest, Vol 171, Issue 4 In-Reply-To: References: Message-ID: Hello all, and especially those that offered advice. Thanks for the advice. I will be experimenting with the options today and tomorrow. Thanks for being so generous with information. Kind regards On Sun, Feb 4, 2018 at 12:00 PM, wrote: > Date: Sat, 3 Feb 2018 13:50:11 -0500 > From: Bob Richmond > > Bryan Llewellyn refers you to > http://stainsfile.info/StainsFile/stain/oversight/romanowsky.htm > > That method prescribes neutral buffered formalin fixation. Us old-timers > preferred Zenker fixation, which you can't do today on account of the > mercury in it. You may have to try different fixatives. > > The differentiation under microscopic control is really necessary - when I > was a resident at Cornell Medical Center in NYC fifty years ago, the > histotechs would push the residents off the double-headed microscopes when > they sat down to differentiate the bone marrow biopsy section Giemsa > stains. > > The old-timers used a 10% solution of colophonium rosin (almost pure > abietic acid) in alcohol, instead of acetic acid, to differentiate the > stain. > > You buy Giemsa or Wright or Romanovsky stain already dissolved in absolute > alcohol. You have to be a fanatic about not contaminating your stock bottle > with the tiniest trace of water. > > Bob Richmond > Samurai Pathologist > Maryville TN > -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org ******************************************************************************** Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. *Please note: I don't usually check my email over the weekend. * From relia1 at earthlink.net Tue Feb 6 10:22:40 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 6 Feb 2018 11:22:40 -0500 Subject: [Histonet] A quick note on a Post Super Bowl Tuesday! Message-ID: <000001d39f66$b608b1a0$221a14e0$@earthlink.net> Hello Histonetters, I thought I would drop you a line while we all "recover" Super Bowl Sunday! Which did you prefer - the game, the halftime show, the snacks or the commercials? Whether you are a football fan or not I hope you had a great weekend and are looking forward to a fantastic week! Since its only Tuesday and my phone is ringing off the hook with new opportunities I wanted to do a quick post to tell you about the positions that I am working on and am MOST excited about. Why am I excited about these positions? Because these clients offer excellent compensation, benefits, relocation assistance and growth! But most importantly they are ready to interview and hire ASAP. All of these positions are full time and permanent. These are the histology positions that I am most excited about! LEADERSHIP/SUPERVISORY: Product Manager IHC/Molecular - San Diego, CA - A RELIA EXCLUSIVE!! Histology Supervisor - Tampa, FL Histology Supervisor - Birmingham, AL "brand new lab" Field Application Specialist - San Diego OR San Francisco, CA TECHNICIANS/TECHNOLOGISTS: Madison, WI Kansas City, MO Austin, TX Birmingham, AL San Diego, CA Syracuse, NY Charlotte, NC San Francisco, CA If you happen to know anyone, I am also recruiting for: . Pathologists Assistant in Kansas City, MO . Grossing Technicians in Kansas City, MO If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I place someone you refer, You will earn a referral fee. I can be reached toll free at the office at 866-607-3542 Or Email me at relia1 at earthlink.net Or You can always catch me on cell-call or text me at 407-353-5070. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From drmoses111 at comcast.net Tue Feb 6 18:15:40 2018 From: drmoses111 at comcast.net (RENEE MOSES) Date: Tue, 6 Feb 2018 19:15:40 -0500 (EST) Subject: [Histonet] Histology Job in South Jersey Message-ID: <1759115019.15817.1517962541237@connect.xfinity.com> Virtua Health, Voorhees, N.J., has a second shift histology position opening available, experience and HT certification preferred. Please contact: Roxann Thompson, HTL rthompson at virtua.org mailto:rthompson at virtua.org (856) 247-3144 From cls71877 at gmail.com Wed Feb 7 09:52:11 2018 From: cls71877 at gmail.com (Cristi Rigazio) Date: Wed, 7 Feb 2018 10:52:11 -0500 Subject: [Histonet] Digital path Message-ID: <642AE40A-3118-49FF-BCE9-B78E362611CB@gmail.com> Hi histoland, I am exploring digital pathology and I am unclear about the current CAP requirements and how those may change if the platform is FDA approved for primary diagnosis. Is the wash out period still necessary if it?s not considered and LDT? Does the personnel still have to be qualified as high complexity testing personnel to scan the slides? Any help or other information is greatly appreciated! Thanks, Cristi Sent from my iPhone From raestask at grics.net Wed Feb 7 10:18:20 2018 From: raestask at grics.net (raestask) Date: Wed, 07 Feb 2018 10:18:20 -0600 Subject: [Histonet] Joint Commission Message-ID: <5D.DD.18911.EC62B7A5@smtp04.onyx.dfw.sync.lan> Has anyone heard of a regulation by the Joint Commission to require grossing be done in a negative pressure room? Rae StaskiewiczUnity point Health?MethodistPeoria, Il Sent from my Verizon, Samsung Galaxy smartphone From Valerie.Hannen at parrishmed.com Wed Feb 7 12:31:45 2018 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Wed, 7 Feb 2018 13:31:45 -0500 Subject: [Histonet] [External Sender] Joint Commission In-Reply-To: <5D.DD.18911.EC62B7A5@smtp04.onyx.dfw.sync.lan> References: <5D.DD.18911.EC62B7A5@smtp04.onyx.dfw.sync.lan> Message-ID: <450B7A81EDA0C54E97C53D60F00776C32410E26DF3@isexstore03> Our grossing area is in a negative pressure room due to the formalin, alcohol and xylene fumes. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com -----Original Message----- From: raestask via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, February 07, 2018 11:18 AM To: histonet at lists.utsouthwestern.edu Subject: [External Sender] [Histonet] Joint Commission WARNING: This message came from an external source. Please do not click links or open attachments if unexpected or unusual. Has anyone heard of a regulation by the Joint Commission to require grossing be done in a negative pressure room? Rae StaskiewiczUnity point Health MethodistPeoria, Il Sent from my Verizon, Samsung Galaxy smartphone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From CBird at amli-denton.com Wed Feb 7 12:49:07 2018 From: CBird at amli-denton.com (Cindy Bird) Date: Wed, 7 Feb 2018 12:49:07 -0600 Subject: [Histonet] antibody washing off Message-ID: Hello All, Anyone out there having issues with patient samples washing off on the Agilent Autostainer Plus? We are experiencing patient tissue washing off but the negative (same tissue sample) is staying on? Cindy From badams at acadianagastro.com Wed Feb 7 13:30:14 2018 From: badams at acadianagastro.com (Brent Adams) Date: Wed, 7 Feb 2018 19:30:14 +0000 Subject: [Histonet] Histonet Digest, Vol 171, Issue 7 Topic 3 In-Reply-To: References: Message-ID: Rae, My lab was set up new in 2012 with negative pressure air flow with 100% make up air through the HVAC system. The contractors used current Laboratory ventilation protocols as I understand it and it included negative pressure throughout the lab. The purpose is to prevent the fumes from Formalin and other chemical in the lab from seeping out from under the doors and through wall cracks or windows to other populated areas exposing others to known carcinogens and fowl smelling fumes such as xylene and alcohol. I still have to work under a hood to protect my self from formalin exposure per OSHA protocols. Hope this helped. Brent Adams ? BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Wednesday, February 7, 2018 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 171, Issue 7 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Histology Job in South Jersey (RENEE MOSES) 2. Digital path (Cristi Rigazio) 3. Joint Commission (raestask) ---------------------------------------------------------------------- Message: 1 Date: Tue, 6 Feb 2018 19:15:40 -0500 (EST) From: RENEE MOSES To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Histology Job in South Jersey Message-ID: <1759115019.15817.1517962541237 at connect.xfinity.com> Content-Type: text/plain; charset=UTF-8 Virtua Health, Voorhees, N.J., has a second shift histology position opening available, experience and HT certification preferred. Please contact: Roxann Thompson, HTL rthompson at virtua.org mailto:rthompson at virtua.org (856) 247-3144 ------------------------------ Message: 2 Date: Wed, 7 Feb 2018 10:52:11 -0500 From: Cristi Rigazio To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Digital path Message-ID: <642AE40A-3118-49FF-BCE9-B78E362611CB at gmail.com> Content-Type: text/plain; charset=utf-8 Hi histoland, I am exploring digital pathology and I am unclear about the current CAP requirements and how those may change if the platform is FDA approved for primary diagnosis. Is the wash out period still necessary if it?s not considered and LDT? Does the personnel still have to be qualified as high complexity testing personnel to scan the slides? Any help or other information is greatly appreciated! Thanks, Cristi Sent from my iPhone ------------------------------ Message: 3 Date: Wed, 07 Feb 2018 10:18:20 -0600 From: raestask To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Joint Commission Message-ID: <5D.DD.18911.EC62B7A5 at smtp04.onyx.dfw.sync.lan> Content-Type: text/plain; charset=utf-8 Has anyone heard of a regulation by the Joint Commission to require grossing be done in a negative pressure room? Rae StaskiewiczUnity point Health?MethodistPeoria, Il Sent from my Verizon, Samsung Galaxy smartphone ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 171, Issue 7 **************************************** PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. From lkc0001 at auburn.edu Thu Feb 8 07:12:08 2018 From: lkc0001 at auburn.edu (Lisa Parsons) Date: Thu, 8 Feb 2018 13:12:08 +0000 Subject: [Histonet] DAKO Message-ID: Has anyone had any issues with their service/contracts from DAKO now that they are just Agilent? My new contract is very disappointing. From mills at 3scan.com Thu Feb 8 12:10:44 2018 From: mills at 3scan.com (Caroline Miller) Date: Thu, 8 Feb 2018 10:10:44 -0800 Subject: [Histonet] DAKO In-Reply-To: References: Message-ID: I have issues with the fact that the website no longer has datasheets available and you have to email the customer services to get them!! It has been like that for almost a year, even though they say they are working on it. yours, mills On Thu, Feb 8, 2018 at 5:12 AM, Lisa Parsons via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Has anyone had any issues with their service/contracts from DAKO now that > they are just Agilent? My new contract is very disappointing. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 From jqb7 at cdc.gov Thu Feb 8 12:36:48 2018 From: jqb7 at cdc.gov (Sanders, Jeanine (CDC/OID/NCEZID)) Date: Thu, 8 Feb 2018 18:36:48 +0000 Subject: [Histonet] DAKO In-Reply-To: References: Message-ID: <5ba05505c6774744889aab0230f61987@cdc.gov> I mentioned the website was not customer friendly for the Dako products and I also was told they were working on it. Jeanine H. Sanders Infectious Diseases Pathology Branch Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS-G32 Atlanta, GA 30329 -----Original Message----- From: Caroline Miller via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, February 8, 2018 1:11 PM To: Lisa Parsons Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] DAKO I have issues with the fact that the website no longer has datasheets available and you have to email the customer services to get them!! It has been like that for almost a year, even though they say they are working on it. yours, mills On Thu, Feb 8, 2018 at 5:12 AM, Lisa Parsons via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Has anyone had any issues with their service/contracts from DAKO now > that they are just Agilent? My new contract is very disappointing. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy_Schmitt at pa-ucl.com Thu Feb 8 13:11:12 2018 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Thu, 8 Feb 2018 19:11:12 +0000 Subject: [Histonet] cassette labeling Message-ID: Hello- Is anyone out there using the PH1 or PH6 cassette labelers from General Data? The technology is different from the original and we are looking to upgrade. I shall appreciate any information/comments you are willing to share. Thank You, Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From criley at dpspa.com Fri Feb 9 14:21:59 2018 From: criley at dpspa.com (Charles Riley) Date: Fri, 9 Feb 2018 15:21:59 -0500 Subject: [Histonet] Control blocks Message-ID: Does anyone have any extra control block(s) for Gal-3 and or HBME-1 IHC's? We are running out of our last one and trying not to purchase pre-made slides. We use them on our FNA cases but the paths don't want to use the FNA's as control tissue If you have any you can spare it would be appreciated. Please e-mail me for more details. I will cut a few slides from the block and return it with plenty of tissue back to you for storage purposes unless you don't need it back. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From patpxs at gmail.com Fri Feb 9 17:48:29 2018 From: patpxs at gmail.com (P Sicurello) Date: Fri, 9 Feb 2018 15:48:29 -0800 Subject: [Histonet] Service person for Zeiss 10C TEM Message-ID: Good Afternoon, Does anyone know of a service engineer that works on old electron microscopes? I have a Zeiss 10C and someone else I know has one too and they both broke at the same time. If you know of someone, please send me their contact information. Thank you and have a nice weekend. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From gu.lang at gmx.at Sat Feb 10 02:36:16 2018 From: gu.lang at gmx.at (Gudrun Lang) Date: Sat, 10 Feb 2018 09:36:16 +0100 Subject: [Histonet] new developments in histopathology? Message-ID: <000001d3a24a$38e92740$aabb75c0$@gmx.at> Dear histonetters, I have to do financial planning for our diagnostic histolab until 2023 for the necessitiy of new instruments etc. As far as diagnostic development is a rather fast thing, I hardly can imagine what will aproach us in 5 years in the clinical set. What do you think? Which emerging technologies, besides the whole molecularpathology, will be used in histomorphology. (in situ PCR? Mutation-specific-in situ PCR?, automated FISH, .) Many thanks for your inputs! Gudrun Lang (Linz, Austria) From Scott.Lindrud at rice.willmar.mn.us Mon Feb 12 09:37:41 2018 From: Scott.Lindrud at rice.willmar.mn.us (Lindrud, Scott) Date: Mon, 12 Feb 2018 15:37:41 +0000 Subject: [Histonet] MMR Negative controls Message-ID: <0fd3ac7b263b4203a58f806118258735@RMH-MAIL.ricehospital.local> Good Morning HistoNet, Would anyone be willing to share what they use for control tissue for MMR testing? Finding negative expressing tumor is becoming more difficult for us to find and we're wondering if there is an alternate negative tissue that would be acceptable. NordiQC recommends using Tonsil as a positive tissue control and Colon Adenocarcinoma that has a loss of expression as a negative tissue control for all four antibodies (MLH1, MSH2, MSH6, PMS2). Stromal cells in the negative tissue control should have positive nuclear staining and serve as a positive internal control. NordiQC doesn't have any recommendations for alternative tissue to use as a negative control. Anyone doing anything differently? Thanks, Scott Scott A. Lindrud, MLS(ASCP)CM CTCM Histopathology Technical Specialist/Cytotechnologist Rice Memorial Hospital 301 Becker Ave SW Willmar, MN 56201 WP(320)231-4406 Fax(320)231-4861 scott.lindrud at rice.willmar.mn.us ________________________________ "This e-mail transmission is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution violates confidentiality and privacy laws and is prohibited. If you are not the intended recipient, please notify the sender immediately and delete all copies of the message. Thank you." From relia1 at earthlink.net Mon Feb 12 10:50:09 2018 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 12 Feb 2018 11:50:09 -0500 Subject: [Histonet] RELIA Special Job Alert Exciting Opportunities Coast to Coast Managers, Supervisors and a little something different! 2-12-2018 Message-ID: <0a3701d3a421$8aede980$a0c9bc80$@earthlink.net> Hello Histonetters, I hope you are having a wonderful day. I am reaching out to you in hopes that you or someone you know may be interested in a new leadership position. I have several exciting opportunities for experienced Managers and Supervisors in hospital and private lab environments in several locations nationwide. These are some of the premier employers in the United States. The positions are of course full time and permanent and offer attractive compensation great benefits and relocation assistance. Here is a list of our current leadership opportunities: Histology Manager - San Francisco Bay Area Histology Manager - Tampa, FL Product Manager - San Francisco Bay Area Histology Supervisor - Birmingham, AL Field Applications Specialist - San Diego or San Francisco Team Lead Histology - Birmingham, AL Our phones are ringing off the hook and we have several confidential management searched getting ready to start so if you are interested in a new management opportunity but don't see what you are looking for please feel free to drop me a line and let me know what to be on the lookout for, for you! If you would like more information or know of someone else who might be interested, please contact me at relia1 at earthlink.net or 866-607-3542. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam RELIA Offers A $500 Referral Bonus For Anyone You Refer And We Place!! There are a lot of recruiters out there right now trying to work with histo techs and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 10 years I have dedicated my practice solely to placing histology professionals like you. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From allanw at biomax.us Mon Feb 12 10:50:08 2018 From: allanw at biomax.us (Allan Wang) Date: Mon, 12 Feb 2018 11:50:08 -0500 Subject: [Histonet] MMR Negative controls In-Reply-To: <0fd3ac7b263b4203a58f806118258735@RMH-MAIL.ricehospital.local> References: <0fd3ac7b263b4203a58f806118258735@RMH-MAIL.ricehospital.local> Message-ID: Hi, We (at US Biomax) recently screened our colon TMAs for loss of expression for MLH1, MSH2, MSH6, PMS2 using Ventana's antibodies. If you need around 10 cases of negative tissue: CO20813a, 208 cases of colon, contains loss of: PMS2: 35 MLH1: 18 MSH6: 14 MSH2: 8 This TMA is not listed on our website but is similar in design to CO20813. We have additional results on other 208 case TMAs too. Feel free to contact me directly for more details. Allan Allan Wang Lab Manager US Biomax, Inc. On Mon, Feb 12, 2018 at 10:37 AM, Lindrud, Scott via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good Morning HistoNet, > > Would anyone be willing to share what they use for control tissue for MMR > testing? Finding negative expressing tumor is becoming more difficult for > us to find and we're wondering if there is an alternate negative tissue > that would be acceptable. > > NordiQC recommends using Tonsil as a positive tissue control and Colon > Adenocarcinoma that has a loss of expression as a negative tissue control > for all four antibodies (MLH1, MSH2, MSH6, PMS2). Stromal cells in the > negative tissue control should have positive nuclear staining and serve as > a positive internal control. NordiQC doesn't have any recommendations for > alternative tissue to use as a negative control. > > Anyone doing anything differently? > > Thanks, > > Scott > > Scott A. Lindrud, MLS(ASCP)CM CTCM > Histopathology Technical Specialist/Cytotechnologist > Rice Memorial Hospital > 301 Becker Ave SW > Willmar, MN 56201 > WP(320)231-4406 > Fax(320)231-4861 > scott.lindrud at rice.willmar.mn.us > > > ________________________________ > > "This e-mail transmission is for the sole use of the intended recipient(s) > and may contain confidential and privileged information. Any unauthorized > review, use, disclosure, or distribution violates confidentiality and > privacy laws and is prohibited. If you are not the intended recipient, > please notify the sender immediately and delete all copies of the message. > Thank you." > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jkempf80 at uga.edu Mon Feb 12 14:02:32 2018 From: jkempf80 at uga.edu (Jennifer Kempf) Date: Mon, 12 Feb 2018 20:02:32 +0000 Subject: [Histonet] Job Opening in Athens, GA Message-ID: Hi...Is it OK to post a position opening? If so, can you please include this in the next histonet email? Thanks so much!! Histology/Grossing technician position open at the University of Georgia, College of Vet Med. This position is primarily for the Grossing Lab, but will have regular rotations through the histology side as well. Posting available here: https://www.ugajobsearch.com/postings/17821. The University of Georgia is an Equal Opportunity/Affirmative Action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, disability, gender identity, sexual orientation or protected veteran status. Jennifer M Kempf Histology Laboratory Manager Department of Pathology College of Veterinary Medicine University of Georgia 501 D.W. Brooks Drive Athens, Georgia 30602 Ph: 706-542-2218 Fax: 706-542-5828 jkempf80 at uga.edu From Reuel.Cornelia at tsrh.org Mon Feb 12 15:07:15 2018 From: Reuel.Cornelia at tsrh.org (Reuel Cornelia) Date: Mon, 12 Feb 2018 21:07:15 +0000 Subject: [Histonet] Deplastifying MMA Message-ID: Hello histonetters, I have a problem with my MMA plastic section on my slide. I could not remove the plastic MMA even if I leave the section slides in Xylene for several days with heat incubation at 60 degrees. This is the first time that happened to my section for several years of doing the same thing over. I was thinking that it was my Xylene lot number so I tried to change it but still does not remove the plastic, and then Itried different solvent Acetone, and Ethylene glycol Monoethyl ether but still it does not work. Can I ask for help if anyone knows what was going on and what would be the best way to remove this plastic from my section? Just to give you my embedding solution was MMA -94% , dibutly phthalate- 5% and perkadox 16- 0.5%. This have been my solution for years and I do not have any problem with the removal of plastic section until now. I was thinking that my MMA (M55909) different lot number from Sigma Aldrich may have cause this because even I tried to use the MMA to dissolve my plastic it does not work. Thank you for and any opinions or protocols are greatly appreciated. Reuel Cornelia TSRH 214-559-7766 ---------------------------------------------------------------------- Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. From Reuel.Cornelia at tsrh.org Mon Feb 12 16:36:01 2018 From: Reuel.Cornelia at tsrh.org (Reuel Cornelia) Date: Mon, 12 Feb 2018 22:36:01 +0000 Subject: [Histonet] Depalstify Message-ID: Hello histonetters, Regarding my first email. The thickness of my section is 5 micron. Reuel I have a problem with my MMA plastic section on my slide. I could not remove the plastic MMA even if I leave the section slides in Xylene for several days with heat incubation at 60 degrees. This is the first time that happened to my section for several years of doing the same thing over. I was thinking that it was my Xylene lot number so I tried to change it but still does not remove the plastic, and then Itried different solvent Acetone, and Ethylene glycol Monoethyl ether but still it does not work. Can I ask for help if anyone knows what was going on and what would be the best way to remove this plastic from my section? Just to give you my embedding solution was MMA -94% , dibutly phthalate- 5% and perkadox 16- 0.5%. This have been my solution for years and I do not have any problem with the removal of plastic section until now. I was thinking that my MMA (M55909) different lot number from Sigma Aldrich may have cause this because even I tried to use the MMA to dissolve my plastic it does not work. Thank you for and any opinions or protocols are greatly appreciated. Reuel Cornelia TSRH 214-559-7766 ---------------------------------------------------------------------- Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. From mgflem at gmail.com Tue Feb 13 08:31:03 2018 From: mgflem at gmail.com (Matthew Fleming) Date: Tue, 13 Feb 2018 08:31:03 -0600 Subject: [Histonet] histotech needed in Milwaukee, WI Message-ID: A histotech is needed for an independent dermatopathology lab near Milwaukee, WI. The lab is located in Brown Deer, just north of Milwaukee. Dermatopathology experience is not required but desirable. Qualification for high complexity testing under CLIA, which is not the same as histotech certification but often comes with it, is desirable. Hours are flexible and negotiable. The working environment is pleasant and the salary and benefits competitive. If interested, please email Dr. Matthew Fleming at mf at flemingdp.com. From relia1 at earthlink.net Tue Feb 13 11:14:36 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 13 Feb 2018 12:14:36 -0500 Subject: [Histonet] Happy Mardi Gras! Let the Good Times Roll! Message-ID: <000001d3a4ee$1ff75170$5fe5f450$@earthlink.net> Hi Histonetters! Happy Mardi Gras!!!! Are you doing anything special to celebrate Mardi Gras? New Orleans has had a special place in my heart for a long time. My husband proposed to me there many years ago and we try to get there at least once a year. Since we won?t be in NOLA for Mardi Gras this year we found a Mardi Gras block party to go to here in town. So Laissez Les Bon Temps Rouler! That means: Let the good times roll!! I hope you have a chance to add some fun and frivolity to your day!! Instead of asking YOU to ?THROW ME SOMETHING MISTER? Please take a look at my current job openings! All of these are permanent full time openings. I am proud to say that MOST of these are RELIA EXCLUSIVES and all of my clients offer competitive pay, great benefits and great people to work with. Most offer Relocation assistance or a Sign on Bonus8 Histology /Lead/ Supervisor/Management: San Francisco Bay Area Tampa, FL Birmingham, AL San Diego, CA Histotechnicians/Histotechnologists: Kansas City, MO Madison, WI Austin, TX Charlotte, NC San Diego, CA Syracuse, NY If you or anyone you know is interested in hearing more about any of the opportunities or exploring a job search in another area please contact me. You can reach me at relia1 at earthlink.net or toll free at 866-607-3542 or on my cell at 407-353-5070. I will be happy to help, everything is kept in strictest of confidence and if I place someone you refer to me you will earn a referral fee. Thanks-Pam Right Place, Right Time, Right Move with RELIA! March 9, 2018 is Histotechnology Professionals Day. Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From redrose297 at gmail.com Tue Feb 13 13:37:06 2018 From: redrose297 at gmail.com (warda hassan) Date: Tue, 13 Feb 2018 19:37:06 +0000 Subject: [Histonet] Gemini versus shandan auto Stainer Message-ID: Dear all Hope all are doing good, wanted to ask and seek advice of anyone of the members had hand on experience on H/E autistainer; 1-thermo shandon Gemini auto Stainer 2-dako autistainer & cover slippers Thank you all Warda From Tom_Wells at bcit.ca Tue Feb 13 15:17:34 2018 From: Tom_Wells at bcit.ca (Tom Wells) Date: Tue, 13 Feb 2018 21:17:34 +0000 Subject: [Histonet] External Quality Assurance Programs Message-ID: <58c39cdab6c1484ea22d406cdb048c7b@ex2013-01.ad.bcit.ca> Hi, Some time ago I discovered an incredibly useful teaching resource. I believe it was part of an external quality assurance program for Histopathology. It had a large collection of images from materials that they had received for evaluation. These images were from all areas such as Immunohistochemistry, special stains, H&E, etc. They included ratings that they had given them as feedback. Including all mistakes that they had observed. Unfortunately, I didn't keep track of the site and I can no longer find it. If anyone can recall seeing this site I would appreciate it since it would take a lifetime to deliberately recreate all of the mistakes. Thanks. Tom Tom Wells BSc, MEd, MLT, ART Faculty Medical Laboratory Science School of Health Sciences SW03-3088 (604) 412-7594 BCIT From cforster at umn.edu Tue Feb 13 21:46:46 2018 From: cforster at umn.edu (Colleen Forster) Date: Tue, 13 Feb 2018 21:46:46 -0600 Subject: [Histonet] processing Dosophilla flies Message-ID: Can anyone give me a processing schedule for Drosophila flies...very tiny. Thank you in advance! Colleen Forster HT(ASCP)QIHC U of MN From seaduster at gmail.com Wed Feb 14 02:11:15 2018 From: seaduster at gmail.com (seaduster at gmail.com) Date: Wed, 14 Feb 2018 08:11:15 +0000 Subject: [Histonet] (no subject) Message-ID: <861657ex97qm$etgvteqa$ha277s0x$@daltron.com.pg> Hey Histonet https://goo.gl/pmPPmg From carl.hobbs at kcl.ac.uk Wed Feb 14 13:36:23 2018 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Wed, 14 Feb 2018 19:36:23 +0000 Subject: [Histonet] processing Dosophilla flies Message-ID: Hi Processing them , how? To Pwax? To snapfreeze? To resin? Kind regards Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From abtdhu at gmail.com Wed Feb 14 13:45:50 2018 From: abtdhu at gmail.com (Dorothy Hu) Date: Wed, 14 Feb 2018 14:45:50 -0500 Subject: [Histonet] Deplastifying MMA Message-ID: > > Hi Reuel > We had the same thing as you. It was caused by bad MMA, I tested. Write down the lot # and ask refund. Test new lot # 1st of MMA in the future. It was very frustrating....I know. Best luck. Dorothy > Message: 2 > Date: Mon, 12 Feb 2018 21:07:15 +0000 > From: Reuel Cornelia > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Deplastifying MMA > Message-ID: > namprd17.prod.outlook.com> > > Content-Type: text/plain; charset="iso-8859-1" > > Hello histonetters, > > I have a problem with my MMA plastic section on my slide. I could not > remove the plastic MMA even if I leave the section slides in Xylene for > several days with heat incubation at 60 degrees. This is the first time > that happened to my section for several years of doing the same thing over. > I was thinking that it was my Xylene lot number so I tried to change it but > still does not remove the plastic, and then Itried different solvent > Acetone, and Ethylene glycol Monoethyl ether but still it does not work. > Can I ask for help if anyone knows what was going on and what would be the > best way to remove this plastic from my section? > > Just to give you my embedding solution was MMA -94% , dibutly phthalate- > 5% and perkadox 16- 0.5%. This have been my solution for years and I do not > have any problem with the removal of plastic section until now. I was > thinking that my MMA (M55909) different lot number from Sigma Aldrich may > have cause this because even I tried to use the MMA to dissolve my plastic > it does not work. > > > Thank you for and any opinions or protocols are greatly appreciated. > > > Reuel Cornelia > > TSRH > > 214-559-7766 > > From lori.garcia at medtronic.com Wed Feb 14 14:05:16 2018 From: lori.garcia at medtronic.com (Garcia, Lori, M.Sc.) Date: Wed, 14 Feb 2018 20:05:16 +0000 Subject: [Histonet] [EXTERNAL] Deplastifying MMA In-Reply-To: References: Message-ID: Hi Reuel, One thing you could try is THF (tetrahydrofuran). We have used it in the past to rapidly dissolve the MMA on slides. Good luck, Lori -----Original Message----- From: Dorothy Hu via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, February 14, 2018 11:46 AM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] Deplastifying MMA > > Hi Reuel > We had the same thing as you. It was caused by bad MMA, I tested. Write down the lot # and ask refund. Test new lot # 1st of MMA in the future. It was very frustrating....I know. Best luck. Dorothy > Message: 2 > Date: Mon, 12 Feb 2018 21:07:15 +0000 > From: Reuel Cornelia > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Deplastifying MMA > Message-ID: > namprd17.prod.outlook.com> > > Content-Type: text/plain; charset="iso-8859-1" > > Hello histonetters, > > I have a problem with my MMA plastic section on my slide. I could not > remove the plastic MMA even if I leave the section slides in Xylene > for several days with heat incubation at 60 degrees. This is the first > time that happened to my section for several years of doing the same thing over. > I was thinking that it was my Xylene lot number so I tried to change > it but still does not remove the plastic, and then Itried different > solvent Acetone, and Ethylene glycol Monoethyl ether but still it does not work. > Can I ask for help if anyone knows what was going on and what would be > the best way to remove this plastic from my section? > > Just to give you my embedding solution was MMA -94% , dibutly > phthalate- 5% and perkadox 16- 0.5%. This have been my solution for > years and I do not have any problem with the removal of plastic > section until now. I was thinking that my MMA (M55909) different lot > number from Sigma Aldrich may have cause this because even I tried to > use the MMA to dissolve my plastic it does not work. > > > Thank you for and any opinions or protocols are greatly appreciated. > > > Reuel Cornelia > > TSRH > > 214-559-7766 > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com From michael.gudo at morphisto.de Wed Feb 14 14:50:13 2018 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Wed, 14 Feb 2018 21:50:13 +0100 Subject: [Histonet] Deplastifying MMA In-Reply-To: References: Message-ID: Hi Dorothy, Hi Reuel, MMA embedding and deplastination is of course a very difficult process and many protocols are discussed, but only a few work well. So in general: real deplastination cannot be performed by xylene or acetone alone, these solutions are just a pretreatment. The best way for remove MMA from section is a procedure starting with xylene, then going into acetone (2 times) and then into MEA (Methoxyethylacetate) for at least 2-3 times for a minimum of 10-20 minutes. You can also directly go into MEA, however, pretreatment with xylene or acetone helps to reduce the steps and volume of MEA you need. Which kind of MMA do you use? In our lab we do resin embedding in Technovit 9100, which is very well established MMA that provides good results for thin sections between around a few microns, but and also for thicker sections and even for cutting-grinding-techniques. We can also provide you with several protocols for infiltration, deplastination and staining of Technovit-sections. You find some further information and also some hands-on-descriptions for all histology-technovits in this brochure: http://www.morphisto.de/fileadmin/pdf/Brochure_Technovit_Histology_EN.pdf If you have any further questions, just contact me personally. With best regards Michael P.S. Further information about the technovits you can also find here: http://www.morphisto.de/en/resin-histology/technovit-resins/ > Am 14.02.2018 um 20:45 schrieb Dorothy Hu via Histonet >: > >> >> Hi Reuel >> > > We had the same thing as you. It was caused by bad MMA, I tested. Write > down the lot # and ask refund. Test new lot # 1st of MMA in the future. It > was very frustrating....I know. > Best luck. > > Dorothy > > > > > >> Message: 2 >> Date: Mon, 12 Feb 2018 21:07:15 +0000 >> From: Reuel Cornelia > >> To: "histonet at lists.utsouthwestern.edu " >> > >> Subject: [Histonet] Deplastifying MMA >> Message-ID: >> > namprd17.prod.outlook.com > >> >> Content-Type: text/plain; charset="iso-8859-1" >> >> Hello histonetters, >> >> I have a problem with my MMA plastic section on my slide. I could not >> remove the plastic MMA even if I leave the section slides in Xylene for >> several days with heat incubation at 60 degrees. This is the first time >> that happened to my section for several years of doing the same thing over. >> I was thinking that it was my Xylene lot number so I tried to change it but >> still does not remove the plastic, and then Itried different solvent >> Acetone, and Ethylene glycol Monoethyl ether but still it does not work. >> Can I ask for help if anyone knows what was going on and what would be the >> best way to remove this plastic from my section? >> >> Just to give you my embedding solution was MMA -94% , dibutly phthalate- >> 5% and perkadox 16- 0.5%. This have been my solution for years and I do not >> have any problem with the removal of plastic section until now. I was >> thinking that my MMA (M55909) different lot number from Sigma Aldrich may >> have cause this because even I tried to use the MMA to dissolve my plastic >> it does not work. >> >> >> Thank you for and any opinions or protocols are greatly appreciated. >> >> >> Reuel Cornelia >> >> TSRH >> >> 214-559-7766 >> >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. ************************************************************************************************ MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From Nilufa.Sultana at uts.edu.au Wed Feb 14 21:29:09 2018 From: Nilufa.Sultana at uts.edu.au (Nilufa Sultana) Date: Thu, 15 Feb 2018 03:29:09 +0000 Subject: [Histonet] About Amoebae infected Tissue for Undergraduate student Practical class Message-ID: Dear Histo-netter, It will be appreciated if I can get information for the following- 1. Any source to get Amoebae infected Animal/humane tissue/Block to cut about 800 slides for the undergraduate Anatomy Pathology Practical class for staining with PAS 2. Any method to make control block with the infection of Amoebae to the Animal tissue Thank you so much . With kind regards Nilufa Sultana Histology and Anatomy pathology University Of Technology, Sydney Australia UTS CRICOS Provider Code: 00099F DISCLAIMER: This email message and any accompanying attachments may contain confidential information. If you are not the intended recipient, do not read, use, disseminate, distribute or copy this message or attachments. If you have received this message in error, please notify the sender immediately and delete this message. Any views expressed in this message are those of the individual sender, except where the sender expressly, and with authority, states them to be the views of the University of Technology Sydney. Before opening any attachments, please check them for viruses and defects. Think. Green. Do. Please consider the environment before printing this email. From LRaff at uropartners.com Thu Feb 15 08:18:09 2018 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 15 Feb 2018 14:18:09 +0000 Subject: [Histonet] Blog Link Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF11877D92@COLOEXCH01.uropartners.local> An article in the New York Times inspired today's blog. Sorry that no histologists were needed in solving this autopsy riddle! http://www.chicagonow.com/downsize-maybe/2018/02/from-the-autopsy-files-the-triangle-of-death/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From ronald.kusters at pfmmedical.com Fri Feb 16 03:50:15 2018 From: ronald.kusters at pfmmedical.com (Kusters, Ronald) Date: Fri, 16 Feb 2018 09:50:15 +0000 Subject: [Histonet] Deplastifying MMA In-Reply-To: References: Message-ID: <8e78115855214a058d4e6ccc137a2ed6@EX-PFM-103-W.pfm-ag.net> Dear Reuel, Maybe the Kulkzer Technovit manufacturers website provides you more information or you could contact their specialists directly through this link: http://kulzer-technik.de/en_kt/kt/maerkte/histologie/startseite_histologie.aspx Yours, Ronald Mit freundlichen Gr??en/Kind Regards Ronald Kusters International Sales Manager Feather Brand Histotechnology pfm medical ag Wankelstra?e 60 50996 K?ln, Germany T +49 (2236) 9641 660 F +49 (2236) 9641 99 660 M +49 (171) 9144343 -----Original Message----- From: Reuel Cornelia [mailto:Reuel.Cornelia at tsrh.org] Sent: maandag 12 februari 2018 22:07 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Deplastifying MMA Hello histonetters, I have a problem with my MMA plastic section on my slide. I could not remove the plastic MMA even if I leave the section slides in Xylene for several days with heat incubation at 60 degrees. This is the first time that happened to my section for several years of doing the same thing over. I was thinking that it was my Xylene lot number so I tried to change it but still does not remove the plastic, and then Itried different solvent Acetone, and Ethylene glycol Monoethyl ether but still it does not work. Can I ask for help if anyone knows what was going on and what would be the best way to remove this plastic from my section? Just to give you my embedding solution was MMA -94% , dibutly phthalate- 5% and perkadox 16- 0.5%. This have been my solution for years and I do not have any problem with the removal of plastic section until now. I was thinking that my MMA (M55909) different lot number from Sigma Aldrich may have cause this because even I tried to use the MMA to dissolve my plastic it does not work. Thank you for and any opinions or protocols are greatly appreciated. Reuel Cornelia TSRH 214-559-7766 ---------------------------------------------------------------------- Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. From abtdhu at gmail.com Fri Feb 16 07:34:42 2018 From: abtdhu at gmail.com (Dorothy Hu) Date: Fri, 16 Feb 2018 08:34:42 -0500 Subject: [Histonet] [EXTERNAL] Deplastifying MMA Message-ID: Hi Lori, Would you please tell me which company and catalog # of tetrahydrofuran you used before? For how many minutes you did for deplastifying in tetrahydrofuran? Thanks in advance. Dorothy Message: 3 Date: Wed, 14 Feb 2018 20:05:16 +0000 From: "Garcia, Lori, M.Sc." To: "Reuel.Cornelia at tsrh.org" Cc: "histonet at lists.utsouthwestern.edu" Subject: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Reuel, One thing you could try is THF (tetrahydrofuran). We have used it in the past to rapidly dissolve the MMA on slides. Good luck, Lori From jmcgough at clinlab.com Fri Feb 16 10:31:11 2018 From: jmcgough at clinlab.com (=?utf-8?Q?Jason_McGough?=) Date: Fri, 16 Feb 2018 09:31:11 -0700 Subject: [Histonet] P16 Antibody Message-ID: We are looking to bring in P16 to do in house but are having a hard time getting the stain to be clean. We are having issues with positive staining on tissue that should be negative. We are working with a concentrated antibody that we have diluted out to 1:6000 (which is 6 times the amount suggested from vendors 1:200-1:1000). We might need to change clones or vendors and were wondering if you could tell us what kind of p16 (clone) you use for staining and possible what vendor you get it from? Any information or suggestions you have would greatly be appreciated. Thank you. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgough at clinlab.com www.clinlab.com From CMcgrad1 at hurleymc.com Fri Feb 16 12:15:55 2018 From: CMcgrad1 at hurleymc.com (Cynthia McGrady) Date: Fri, 16 Feb 2018 18:15:55 +0000 Subject: [Histonet] used cryostats Message-ID: <0053E7F9DD068946AC3541D0A35C098A0206E5F717@ExchangeMB3.hmc.hurleymc.com> Hi. Our lab is in the market for a used or refurbished cryostat. Any tips or lead would be greatly appreciated. Thank.. Cindy McGrady, Hurley Medical Center, Flint, Michigan. From madeleinehuey at gmail.com Fri Feb 16 12:24:23 2018 From: madeleinehuey at gmail.com (Madeleine Huey) Date: Fri, 16 Feb 2018 10:24:23 -0800 Subject: [Histonet] Histonet Digest, Vol 171, Issue 15 In-Reply-To: References: Message-ID: Subject: [Histonet] P16 Antibody Jason, I also tried many different vendors & clones many years ago (human FFPE tissues), and the winner is from Roche/Ventana (CINtec, Cat. 9517 = 11.5ml). You can get 5ml dispenser as well. *Madeleine Huey**, HTL & QIHC (ASCP)* Supervisor - Pathology (Histology & IPOX) El Camino Hospital, and Los Gatos Hospital 2500 Grant Rd (Rm #GC-413), Mountain View, CA 94040-4378 Phone: 650-940-7038 Fax: 650-966-9292 Email: *madeleine_h at elcaminohospital.org * On Fri, Feb 16, 2018 at 10:00 AM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Deplastifying MMA (Kusters, Ronald) > 2. Re: [EXTERNAL] Deplastifying MMA (Dorothy Hu) > 3. P16 Antibody (Jason McGough) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 16 Feb 2018 09:50:15 +0000 > From: "Kusters, Ronald" > To: Reuel Cornelia , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Deplastifying MMA > Message-ID: <8e78115855214a058d4e6ccc137a2ed6 at EX-PFM-103-W.pfm-ag.net> > Content-Type: text/plain; charset="iso-8859-1" > > Dear Reuel, > Maybe the Kulkzer Technovit manufacturers website provides you more > information or you could contact their specialists directly through this > link: > http://kulzer-technik.de/en_kt/kt/maerkte/histologie/ > startseite_histologie.aspx > > Yours, > Ronald > > Mit freundlichen Gr??en/Kind Regards > > > Ronald Kusters > International Sales Manager Feather Brand Histotechnology > > pfm medical ag > Wankelstra?e 60 > 50996 K?ln, Germany > T +49 (2236) 9641 660 > F +49 (2236) 9641 99 660 > M +49 (171) 9144343 > > > > -----Original Message----- > From: Reuel Cornelia [mailto:Reuel.Cornelia at tsrh.org] > Sent: maandag 12 februari 2018 22:07 > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Deplastifying MMA > > Hello histonetters, > > I have a problem with my MMA plastic section on my slide. I could not > remove the plastic MMA even if I leave the section slides in Xylene for > several days with heat incubation at 60 degrees. This is the first time > that happened to my section for several years of doing the same thing over. > I was thinking that it was my Xylene lot number so I tried to change it but > still does not remove the plastic, and then Itried different solvent > Acetone, and Ethylene glycol Monoethyl ether but still it does not work. > Can I ask for help if anyone knows what was going on and what would be the > best way to remove this plastic from my section? > > Just to give you my embedding solution was MMA -94% , dibutly phthalate- > 5% and perkadox 16- 0.5%. This have been my solution for years and I do not > have any problem with the removal of plastic section until now. I was > thinking that my MMA (M55909) different lot number from Sigma Aldrich may > have cause this because even I tried to use the MMA to dissolve my plastic > it does not work. > > > Thank you for and any opinions or protocols are greatly appreciated. > > > Reuel Cornelia > > TSRH > > 214-559-7766 > > ---------------------------------------------------------------------- > Texas Scottish Rite Hospital for Children is one of the nation's leading > pediatric centers for the treatment of orthopedic conditions, certain > related neurological disorders and learning disorders, such as dyslexia. > This email transmission and/or its attachments may contain confidential > health information, intended only for the use of the individual or entity > named above. > > > > > ------------------------------ > > Message: 2 > Date: Fri, 16 Feb 2018 08:34:42 -0500 > From: Dorothy Hu > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] [EXTERNAL] Deplastifying MMA > Message-ID: > mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hi Lori, > > Would you please tell me which company and catalog # of tetrahydrofuran > you used before? > For how many minutes you did for deplastifying in tetrahydrofuran? > Thanks in advance. > > Dorothy > > Message: 3 > Date: Wed, 14 Feb 2018 20:05:16 +0000 > From: "Garcia, Lori, M.Sc." > To: "Reuel.Cornelia at tsrh.org" > Cc: "histonet at lists.utsouthwestern.edu" > > Subject: > Message-ID: > namprd05.prod.outlook.com> > > Content-Type: text/plain; charset="us-ascii" > > Hi Reuel, > > One thing you could try is THF (tetrahydrofuran). We have used it in the > past to rapidly dissolve the MMA on slides. > > Good luck, > Lori > > > ------------------------------ > > Message: 3 > Date: Fri, 16 Feb 2018 09:31:11 -0700 > From: Jason McGough > To: 'histonet at lists.utsouthwestern.edu' > > Subject: [Histonet] P16 Antibody > Message-ID: > Content-Type: text/plain; charset=utf-8 > > We are looking to bring in P16 to do in house but are having a hard time > getting the stain to be clean. We are having issues with positive staining > on tissue that should be negative. We are working with a concentrated > antibody that we have diluted out to 1:6000 (which is 6 times the amount > suggested from vendors 1:200-1:1000). We might need to change clones or > vendors and were wondering if you could tell us what kind of p16 (clone) > you use for staining and possible what vendor you get it from? Any > information or suggestions you have would greatly be appreciated. Thank you. > > > Jason McGough, HT(ASCP) > > Operations Manager > Clinical Laboratory of the Black Hills > 605-343-2267 > jmcgough at clinlab.com > www.clinlab.com > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 171, Issue 15 > ***************************************** > From boneslides at gmail.com Fri Feb 16 13:22:19 2018 From: boneslides at gmail.com (Diane Mahovlic) Date: Fri, 16 Feb 2018 14:22:19 -0500 Subject: [Histonet] Histonet Digest, Vol 171, Issue 15 In-Reply-To: References: Message-ID: I use methyl acetate to de-plasticize my PMMA slides.. . 30 minutes, air dry for a minute or two then re-hydrate in 100% and 95% alcohol, rinse in water. I make my own PMMA ,rather than using Technovit, maybe that has something to do with it. Has worked for me for 25+ years! Diane Diane Mahovlic, HT(ASCP) Cleveland Clinic, Cleveland, Ohio On Fri, Feb 16, 2018 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Deplastifying MMA (Kusters, Ronald) > 2. Re: [EXTERNAL] Deplastifying MMA (Dorothy Hu) > 3. P16 Antibody (Jason McGough) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 16 Feb 2018 09:50:15 +0000 > From: "Kusters, Ronald" > To: Reuel Cornelia , > "histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Deplastifying MMA > Message-ID: <8e78115855214a058d4e6ccc137a2ed6 at EX-PFM-103-W.pfm-ag.net> > Content-Type: text/plain; charset="iso-8859-1" > > Dear Reuel, > Maybe the Kulkzer Technovit manufacturers website provides you more > information or you could contact their specialists directly through this > link: > http://kulzer-technik.de/en_kt/kt/maerkte/histologie/ > startseite_histologie.aspx > > Yours, > Ronald > > Mit freundlichen Gr??en/Kind Regards > > > Ronald Kusters > International Sales Manager Feather Brand Histotechnology > > pfm medical ag > Wankelstra?e 60 > 50996 K?ln, Germany > T +49 (2236) 9641 660 > F +49 (2236) 9641 99 660 > M +49 (171) 9144343 > > > > -----Original Message----- > From: Reuel Cornelia [mailto:Reuel.Cornelia at tsrh.org] > Sent: maandag 12 februari 2018 22:07 > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Deplastifying MMA > > Hello histonetters, > > I have a problem with my MMA plastic section on my slide. I could not > remove the plastic MMA even if I leave the section slides in Xylene for > several days with heat incubation at 60 degrees. This is the first time > that happened to my section for several years of doing the same thing over. > I was thinking that it was my Xylene lot number so I tried to change it but > still does not remove the plastic, and then Itried different solvent > Acetone, and Ethylene glycol Monoethyl ether but still it does not work. > Can I ask for help if anyone knows what was going on and what would be the > best way to remove this plastic from my section? > > Just to give you my embedding solution was MMA -94% , dibutly phthalate- > 5% and perkadox 16- 0.5%. This have been my solution for years and I do not > have any problem with the removal of plastic section until now. I was > thinking that my MMA (M55909) different lot number from Sigma Aldrich may > have cause this because even I tried to use the MMA to dissolve my plastic > it does not work. > > > Thank you for and any opinions or protocols are greatly appreciated. > > > Reuel Cornelia > > TSRH > > 214-559-7766 > > ---------------------------------------------------------------------- > Texas Scottish Rite Hospital for Children is one of the nation's leading > pediatric centers for the treatment of orthopedic conditions, certain > related neurological disorders and learning disorders, such as dyslexia. > This email transmission and/or its attachments may contain confidential > health information, intended only for the use of the individual or entity > named above. > > > > > ------------------------------ > > Message: 2 > Date: Fri, 16 Feb 2018 08:34:42 -0500 > From: Dorothy Hu > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] [EXTERNAL] Deplastifying MMA > Message-ID: > mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hi Lori, > > Would you please tell me which company and catalog # of tetrahydrofuran > you used before? > For how many minutes you did for deplastifying in tetrahydrofuran? > Thanks in advance. > > Dorothy > > Message: 3 > Date: Wed, 14 Feb 2018 20:05:16 +0000 > From: "Garcia, Lori, M.Sc." > To: "Reuel.Cornelia at tsrh.org" > Cc: "histonet at lists.utsouthwestern.edu" > > Subject: > Message-ID: > namprd05.prod.outlook.com> > > Content-Type: text/plain; charset="us-ascii" > > Hi Reuel, > > One thing you could try is THF (tetrahydrofuran). We have used it in the > past to rapidly dissolve the MMA on slides. > > Good luck, > Lori > > > ------------------------------ > > Message: 3 > Date: Fri, 16 Feb 2018 09:31:11 -0700 > From: Jason McGough > To: 'histonet at lists.utsouthwestern.edu' > > Subject: [Histonet] P16 Antibody > Message-ID: > Content-Type: text/plain; charset=utf-8 > > We are looking to bring in P16 to do in house but are having a hard time > getting the stain to be clean. We are having issues with positive staining > on tissue that should be negative. We are working with a concentrated > antibody that we have diluted out to 1:6000 (which is 6 times the amount > suggested from vendors 1:200-1:1000). We might need to change clones or > vendors and were wondering if you could tell us what kind of p16 (clone) > you use for staining and possible what vendor you get it from? Any > information or suggestions you have would greatly be appreciated. Thank you. > > > Jason McGough, HT(ASCP) > > Operations Manager > Clinical Laboratory of the Black Hills > 605-343-2267 > jmcgough at clinlab.com > www.clinlab.com > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 171, Issue 15 > ***************************************** > From relia1 at earthlink.net Mon Feb 19 11:12:21 2018 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 19 Feb 2018 12:12:21 -0500 Subject: [Histonet] Happy Presidents Day ! HOT NEW HISTOLOGY JOBS 2-19-2018 Message-ID: <000001d3a9a4$ce1f6110$6a5e2330$@earthlink.net> Hello Histonetters, Happy Presidents Day! I have some exciting new opportunities to share!! So after you have hit the Presidents Day Sales and had fun with the kiddos please take a sec to have a look at some of the best opportunities in Histology Nationwide! RELIA'S Hot Histology Opportunities!! IHC Specialist - Los Angeles, CA Histotech - Baltimore, MD Histotech - San Diego, CA - New Lab!! Histology Supervisor - San Franciso Bay Area Histology Tech - Kansas City, MO . Histo Supervisor Tampa, FL . Histotechnician Charlotte, NC . Histology Tech Birmingham, AL dermpath . Histotech San Diego, CA All of these jobs are full time permanent positions with some of the finest facilities in the country. My clients offer excellent compensation and benefits and most offer relocation assistance or sign- on bonuses. For more information for you or a friend please contact me toll free at the office at 866-607-3542 or on my cell call/text at 407-353-5070 or shoot me an email: relia1 at earthlink.net Have a Happy Presidents Day! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From robrankin at rankinbiomed.com Mon Feb 19 14:01:24 2018 From: robrankin at rankinbiomed.com (Rob Rankin) Date: Mon, 19 Feb 2018 15:01:24 -0500 Subject: [Histonet] used cryostats Message-ID: Hi Cindy, I'm glad I saw your post for a used cryostat. Rankin is only 15 miles from you in Michigan and we have been refurbishing histology equipment for over 23 years. We have many cryostats in stock, and I'm sure one will meet your needs. We specialize in core histology equipment and we guarantee our refurbished equipment will provide many years of reliable service. We support our equipment with parts and service; Rankin?s standard 1-year parts and labor warranty is equal to the manufacturer's warranty on new equipment. We also offer trade-in credit if you have used or broken equipment. Give me a call or an email at your earliest convenience and I can give you some possible solutions. Respectfully, *Rob Rankin, MSM, SM(ASCP)* Founder and CEO 248.625.4104 robrankin at rankinbiomed.com From mpence at grhs.net Mon Feb 19 16:20:09 2018 From: mpence at grhs.net (Mike Pence) Date: Mon, 19 Feb 2018 22:20:09 +0000 Subject: [Histonet] CMV antibody Message-ID: I am looking to see which vendor everyone is using for CMV. I would like a pre-dilute and one that is not ASR. Cell Marque offers one but it is an ASR. Michael S. Pence | Supervisor of Laboratory Services Great River Health Systems 1221 S. Gear Ave. | West Burlington, IA 52655 Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557 mpence at grhs.net | www.greatrivermedical.org. www.Facebook.com/GreatRiverHealthSystems | www.Twitter/GreatRiverMed Information in this communication, including attachments, is confidential and intended only for the addressee(s). This communication may contain privileged, confidential, proprietary or trade secret information entitled to protection or exemption from disclosure under law. If you are not an intended recipient, please know that any use, distribution or copying of this communication, or any action taken based on the information in this communication, is unauthorized and may be unlawful. If you received this communication in error, please notify the sender and delete this communication from your device. From hans at histologistics.com Mon Feb 19 20:28:35 2018 From: hans at histologistics.com (Hans B Snyder) Date: Mon, 19 Feb 2018 21:28:35 -0500 Subject: [Histonet] Candida albicans Message-ID: Does anyone have a block or two of Candida albicans to trade? We have a very diverse block collection to trade with. Thank you Hans B Snyder JMD Histology & Histologistics Inc. 151 W Main Street Dudley, MA 01571 Lab - 508-461-7207 C- 508-308-7800 www.histologistics.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. From criley at dpspa.com Mon Feb 19 20:30:51 2018 From: criley at dpspa.com (Charles Riley) Date: Mon, 19 Feb 2018 21:30:51 -0500 Subject: [Histonet] Job Position Message-ID: We are looking to hire a night shift employee at a facility in Dover DE. The job position includes doing routine histology (microtomy, special stains, IHC's, some embedding) and also grossing nightly specimens. The shift is 2-10:30 (sometimes needing to stay late if couriers return late). If you are interested please send me an e-mail back with your information and experience and someone will reach out to you with more details Thanks -- C. Riley BS HT, HTL(ASCP)CM From Carrie.Minikus at unitypoint.org Tue Feb 20 11:40:58 2018 From: Carrie.Minikus at unitypoint.org (Minikus, Carrie A.) Date: Tue, 20 Feb 2018 17:40:58 +0000 Subject: [Histonet] policy for handling bariatric patients Message-ID: <40DFB208CC287B4091BE635AC60402C063A9F4F5@TDCEXCHXM005.ihs.org> Does anyone have a policy to share on the handling of bariatric patients before autopsy? I have seen that it is new on the CAP checklist. We do very few autopsies at our facility but need to adopt a procedure to be in compliance with CAP. Any direction from anyone would be greatly appreciated! Carrie Minikus, MLS(ASCP)CM, HTL(ASCP)CM Histology Manager This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. sections 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From relia1 at earthlink.net Tue Feb 20 11:27:06 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 20 Feb 2018 12:27:06 -0500 Subject: [Histonet] Histo Tech needed in Baltimore area for a growing new lab! Message-ID: <02e401d3aa70$21780850$646818f0$@earthlink.net> Hi Histonetters, How are you? I hope you are having a great week! I was hoping you might be able to help me. I am presently working with a client near Baltimore, MD in need of a histotech. This is a full time permanent position. ASCP certification and being CLIA qualified are required and 1 year of experience in Urology is preferred. You will be performing grossing and routine histology ? cutting, embedding and special stains. The help I need from you is do you know anyone that might be interested in hearing about either of these opportunities? If so could you please forward my e-mail to them? If you are interested in this position please contact me ASAP toll free at 866-607-3542, cell/text 407-353-5070 or via email at relia1 at earthlink.net This company has VERY LOW TURNOVER! And they are growing rapidly so there is lots of opportunity for advancement!! If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won?t release it to anyone without your permission. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Nancy.Stedman at buschgardens.com Tue Feb 20 15:45:09 2018 From: Nancy.Stedman at buschgardens.com (Stedman, Nancy) Date: Tue, 20 Feb 2018 21:45:09 +0000 Subject: [Histonet] "Budget" single slide scanners Message-ID: <394b6a5ec1414a3c87ab8e41b3bfab8c@buschgardens.com> Hi Histonetters - Does anyone have experience with the low cost single slide scanners like the uSCOPE machines? Are they any good? Am looking for a slide scanner but have a limited budget ... Thank you! -Nancy Stedman From erin.mccarthy at tempus.com Wed Feb 21 09:52:49 2018 From: erin.mccarthy at tempus.com (Erin McCarthy) Date: Wed, 21 Feb 2018 09:52:49 -0600 Subject: [Histonet] Whole Slide Imaging Message-ID: Hi All, I am curious if any of you have used a whole slide imager, and if so how did you structure your validation plan? Our lab does not make primary Dx, we review samples for tumor content to use for NGS testing. We record images so we can keep a record of the tissue once we send the slides back to the original facilities. Any questions I will gladly add more! Thanks! -- Erin McCarthy, HT (ASCP) Lead Histotechnician Tempus Labs 600 W. Chicago Ave. Chicago IL 60654 Ph:(312) 638-6344 Ext.3835 -- This email and any attachments may contain privileged and confidential information and/or protected health information (PHI) that is protected by federal and state privacy laws. It is intended solely for the use of Tempus Labs and the recipient(s) named above. Nothing contained in this communication and any attachments thereto is intended to waive any privileges or rights of confidentiality. If you are not the recipient, or the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any review, dissemination, distribution, printing or copying of this email message and/or any attachments is strictly prohibited. * If you have received this transmission in error, please notify us immediately at **(855)-442-8305** and permanently delete this email and any attachments*. From martina.waniewska at gmail.com Wed Feb 21 10:20:39 2018 From: martina.waniewska at gmail.com (=?UTF-8?Q?Martyna_Waniewska=2D=C5=81=C4=99czycka?=) Date: Wed, 21 Feb 2018 17:20:39 +0100 Subject: [Histonet] 25-hydroxyvitamin D3 solution on airway epithelial cell line Message-ID: Hi all, Does anyone have some experience with treatment of the nasal epithelial cell line with 25-hydroxyvitamin D3 solution (739650 ALDRICH- 100 UG/ML IN ETHANOL 98%)? Our concern relay on the presence of the Ethanol in solution, and its percentage (98%). Is there a risk of detrimental effect on cell line or it will not damage them? Further more, should we dilute vit. D in some Lipid solution for the cell line safety? Thank you very much for your answer. best regards Martina Waniewska-Leczycka Poland From katherine at ka-recruiting.com Thu Feb 22 08:04:20 2018 From: katherine at ka-recruiting.com (Katherine Marano) Date: Thu, 22 Feb 2018 09:04:20 -0500 Subject: [Histonet] Print all In new window Histology Supervisor Needed Message-ID: Hi Histonet members! I hope you are doing well. I am a healthcare recruiter and I specialize in placing histology professionals into permanent and full time positions nationwide. I just got a fantastic Histology Supervisor opening in beautiful Tampa, Florida and wanted to know if you may be interested, or if you could recommend anyone? I am also working on some other great openings (see below). My clients are all offering competitive compensation and benefits, and most assist with relocation. If you are interested, please send me a resume directly and a good time to reach you-- I'd love to tell you more! *Histology:* Connecticut - Fairfield - Histotech (night shift) Florida - Tampa - MOHS Tech, operating mobile van (MUST HAVE MOHS EXP) Florida - Tampa - Histology Supervisor Georgia - Atlanta - Histotech (1 f/t night shift Sunday-Thursday 4:30 p.m. to 1:00 a.m. & a PRN) Indiana - East Chicago - Histotech (midnight & evening shifts) Maine - Portland - Histotech II (evening shift) Minnesota - Minneapolis - Histotech (day shift, mon - fri 8a-5p) North Carolina - Charlotte - Histotech New York - New York - Histotech (Bachelor?s & NY License req'd) New York - White Pines- Histotech (1st & 2nd shifts open w/ rotating wknds) (IHC exp req'd) Ohio - Columbus - Histotech (1st shift) Virginia - Richmond - Histotechnologist (days) Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From bcdukes at lexhealth.org Thu Feb 22 12:03:25 2018 From: bcdukes at lexhealth.org (Blake Taylor) Date: Thu, 22 Feb 2018 18:03:25 +0000 Subject: [Histonet] Dako H&E Coverstainer Message-ID: Looking for feedback for anyone using the Dako H&E Coverstainer, pros and cons would be appreciated. Thanks Blake Taylor Surgical Pathology Supervisor Lexington Medical Center 803-936-8214 bcdukes at lexhealth.org PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are confidential property of the sender. The information is intended only for the use to the person to whom it was addressed. Any other interception, copying, accessing, or disclosure of this message is prohibited. The sender takes no responsibility for any unauthorized reliance on this message. If you have received this message in error, please immediately notify the sender and purge the message you received. Do not forward this message without permission. From mgflem at gmail.com Thu Feb 22 19:08:01 2018 From: mgflem at gmail.com (Matthew Fleming) Date: Thu, 22 Feb 2018 19:08:01 -0600 Subject: [Histonet] Part-time histotech needed in Milwaukee, WI Message-ID: A part-time histotech is needed for an independent dermatopathology lab near Milwaukee, WI. The lab is located in Brown Deer, just north of Milwaukee. Dermatopathology experience is not required but desirable. Hours are flexible and negotiable. The working environment is pleasant and the salary and benefits competitive. If interested, please email Dr. Matthew Fleming at mf at flemingdp.com. From cforster at umn.edu Fri Feb 23 18:14:20 2018 From: cforster at umn.edu (Colleen Forster) Date: Fri, 23 Feb 2018 18:14:20 -0600 Subject: [Histonet] atlas for Drosphilia fly Message-ID: Greetings Histonet family, I am looking for a good reference for the hsitology of the Drosphilia fly. I am cutting then and need a reference to correlate my stains to. It wouild be really nice if they showed color p[lates done with H/E stain. Does anyone have a good reference they can recommend? Thank you in advance!~ Respectfully, Colleen Forster U of MN From cforster at umn.edu Fri Feb 23 18:16:29 2018 From: cforster at umn.edu (Colleen Forster) Date: Fri, 23 Feb 2018 18:16:29 -0600 Subject: [Histonet] Resource for setting up a hard tissue lab Message-ID: Greetings Histonetters, Our facility is looking into setting up a hard tissue service. I am in need of a resource that I can talk to for advice on the equipment and supplies we would need to have in a preliminary budget proposal. Any hard tissue experts out there willing to assist me? Thank you in advance! Respectfully and hopeful, Colleen Forster U of MN From histo at pathlab.us Tue Feb 27 06:37:36 2018 From: histo at pathlab.us (Histology) Date: Tue, 27 Feb 2018 12:37:36 +0000 Subject: [Histonet] Special GI fixative Message-ID: <09CFA3F99D5B2B42B88CDFB2FC4CFD8206E00C6E@vdc01.domain.local> Hi all, Does anyone use a special fixative for their GI specimens? I was thinking of trying something called GI fix. Ingredients are basically formalin and zinc chloride. Our tissue is always super brittle and dry even with a very short processing schedule. Any thoughts would be greatly appreciated. Thanks, Mehndi Helgren From Dawn.Olszewski at SGMC.ORG Tue Feb 27 10:34:40 2018 From: Dawn.Olszewski at SGMC.ORG (Olszewski, Dawn) Date: Tue, 27 Feb 2018 16:34:40 +0000 Subject: [Histonet] IHC VALIDATION Message-ID: Good morning Histo Peeps, I was wondering if anyone would be willing to share their protocol on antibody validation for the IHC lab on FFPE tissues. Do you know if there are specific regulations on validation or does each lab/hospital develop their own methods. We are no longer under CAP for inspections, but we are inspected by Joint Commission. If you know of specific regulations could you please site them. We seem to be in disagreement on how this should be done in our lab. Thanks for any and all advice in advance. Sincerely, Dawn Olszewski, HTL(ASCP)QIHC From regan.fulton at gmail.com Tue Feb 27 15:48:23 2018 From: regan.fulton at gmail.com (Fulton Regan) Date: Tue, 27 Feb 2018 13:48:23 -0800 Subject: [Histonet] IHC VALIDATION In-Reply-To: References: Message-ID: <02A86AFA-554E-427D-97D6-EE10D3CC756D@gmail.com> Hi Dawn, I would suggest having a look at this CAP guidance paper, below. If I can be of any assistance, please feel free to contact me. 1. Patrick, L. F. et al. Principles of analytic validation of immunohistochemical assays: Guideline from the College of American Pathologists Pathology and Laboratory Quality Center. Arch. Pathol. Lab. Med. 138, 1432?1443 (2014). Regan Regan Fulton, MD/PhD PhenoPath 551 North 34th Street, Suite 100 Seattle, WA 98103 Phone 206-374-9000 CEO, Array Science, LLC 475 Gate 5 Road, #102 Sausalito, CA 94965 > On Feb 27, 2018, at 8:34 AM, Olszewski, Dawn via Histonet wrote: > > Good morning Histo Peeps, > > I was wondering if anyone would be willing to share their protocol on antibody validation for the IHC lab on FFPE tissues. Do you know if there are specific regulations on validation or does each lab/hospital develop their own methods. We are no longer under CAP for inspections, but we are inspected by Joint Commission. > > If you know of specific regulations could you please site them. We seem to be in disagreement on how this should be done in our lab. > Thanks for any and all advice in advance. > > Sincerely, > > Dawn Olszewski, HTL(ASCP)QIHC > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet