From relia1 at earthlink.net Wed Dec 5 10:35:38 2018 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 5 Dec 2018 11:35:38 -0500 Subject: [Histonet] It's a Virtual Cookie Swap Party!! RELIA Histology Careers Bulletin 12-4-2018 Message-ID: <016d01d48cb8$8e1096f0$aa31c4d0$@earthlink.net> Happy Holidays Histonetters!! I hope you are enjoying a wonderful holiday season.!! I?m thinking about doing a cookie swap again this year. My all-time favorite is a meringue cookie that my mom used to make with crushed candy canes, chocolate chips and walnuts. I will probably make those or some shortbread cookies. What would you make for a cookie swap? What is your favorite Christmas cookie to make or eat? My guilty pleasure this year is ANYTHING with White chocolate and peppermint! Histonetters, if you have a minute I would like to tell you about the opportunities that I am working on. All my clients are willing to move now or wait until after the holidays to accommodate you. My clients offer excellent compensation packages including great pay and benefits and relocation assistance/sign on bonuses. RELIA?s Current Histology Opportunities are located in: ? Austin, TX ? Nacogdoches, TX ? Dallas, TX ? Columbus, OH ? Tallahassee, FL ? Fayetteville, NC ? Springfield, MA ? San Diego, CA ? Annapolis, MD And the phone continues to ring off the hook with new opportunities! So if you or anyone you know might be interested in hearing about any of these opportunities or are looking for a new position in another area, (now or at the beginning of the year)please feel free to contact me or pass along my contact information to them. I would love to send you a referral fee! Thanks and again Happy Holidays to you and yours! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From CBird at amli-denton.com Tue Dec 11 08:35:52 2018 From: CBird at amli-denton.com (Cindy Bird) Date: Tue, 11 Dec 2018 08:35:52 -0600 Subject: [Histonet] Solid Waste Reduction Message-ID: Would anyone be willing to share the policy for " solid waste reduction"? We've had a policy and procedure in place for years but our safety officer is trying to cut down on the cost of such disposable. Any information would be greatly appreciated. Cindy Bird Anatomical Medical Laboratories, Inc. 1600 Scripture Street Denton, TX 76201 940-384-6210 940-384-6000 Fax 940-565-9588 From relia1 at earthlink.net Tue Dec 11 10:32:33 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 11 Dec 2018 11:32:33 -0500 Subject: [Histonet] The 12 Days of Christmas for Histotechs - by Ashley Troutman a repost from the histonet 12/2013 Message-ID: <000501d4916f$1ea6c3f0$5bf44bd0$@earthlink.net> Hi Histonetters, ICYMI ? In case you missed it Here is a gem found on the histonet in 12/2013 from Ashley Troutman The 12 days of Christmas for Histotechs Enjoy Histopeeps: ?Hello all, I hope the holidays are treating everyone well. (Especially in light of us having the most hazardous job in the country...) Here's some fun for the season: The Twelve Days of Histo TWELVE Medical Directors ELEVEN logs-a-printing TEN residents reading NINE frozen sections EIGHT administrators SEVEN stainers beeping SIX techs complaining FIVE I...H...Cs....? :) FOUR calling docs THREE recuts TWO special stains And a block with an H and EEEEE... Please feel free to sing along. Merry Christmas in Histoland!? Ashley Troutman BS, MBA, HT(ASCP) QIHC Histonetters, I have some REALLY Exciting opportunities, Here?s a quick list: Leadership: Histology Territory Sales Manager ? Texas Histology Territory Sales Manager ? Ohio Histology Manager ? Fayetteville, NC Histotechnicians/Histotechnologists North Carolina Wisconsin Massachusetts Florida Texas Maryland California Arkansas I have new stuff coming in all of the time.? Let me know if anything looks interesting on my list or if there is a specific area you would like for me to be on the lookout for.? We can start chatting now about opportunities after the new year!! The early bird gets the worm!! I can be reached asap on my cell/text at 407-353-5070 or email me at relia1 at earthlink.net Happy Holidays to you and yours!!!! Happy Holidays !!!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From c.tague at Pathologyarts.com Wed Dec 12 11:47:51 2018 From: c.tague at Pathologyarts.com (Curt) Date: Wed, 12 Dec 2018 17:47:51 +0000 Subject: [Histonet] BRCA-1 on Benchmark Ultra Message-ID: <9C8F910F72893643B3C3793C3D67132B94CF9984@PATHOLOGYSERVER.pathologyarts.local> I'm trying to get this marker dialed in with little success... would anyone be willing to share their protocol to save me a little time and money? Biocare antibody... working with CC1 and primary AB times.... Any help is greatly appreciated. Thanks, Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. From CDavis at che-east.org Thu Dec 13 09:25:10 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Thu, 13 Dec 2018 15:25:10 +0000 Subject: [Histonet] CAP checklist for temperature and humidity Message-ID: <6b9172bc7f7043719a576a7446abb738@che-east.org> Good morning folks, I need some help. You folks were wonderful helping with the optimal ranges earlier this year and I tried to find it before I asked but we are not "finding a specific CAP requirement telling us to monitor the humidity as well as temperature in the histology lab only the slide storage room". Most histology people know how humidity can affect microtomy and section tissue adhesion, I would think it would be a requirement but "they" say there is no specific requirement and "they" are not convinced the per manufacture instrument operation requirements qualifies. Any help you can provide is appreciated, my head hurts from beating it against the wall. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From katherine at ka-recruiting.com Thu Dec 13 09:36:57 2018 From: katherine at ka-recruiting.com (Katherine Marano) Date: Thu, 13 Dec 2018 10:36:57 -0500 Subject: [Histonet] Histotech opening in the New Year Message-ID: Hi Histonetters! My client in Salem, Oregon is looking to hire a full-time and permanent Histotech. The shift is likely 9am-5:30pm. They are looking for an experienced tech, with QIHC or IHC experience preferred. Are you, or do you know of anyone who may be, interested? I'd love to tell you more! Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 katherine at ka-recruiting.com http://www.ka-recruiting.com From Richard.Cartun at hhchealth.org Fri Dec 14 14:38:38 2018 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 14 Dec 2018 20:38:38 +0000 Subject: [Histonet] IDH1 Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EAC0E2FD1@HHCEXCHMB03.hhcsystem.org> For those labs that are performing IDH1 IHC testing on FFPE tissue, which clone are you using and where do you get it? Thank you. Happy Holidays to everyone. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From annigyg at gmail.com Sat Dec 15 19:15:47 2018 From: annigyg at gmail.com (Anne van Binsbergen) Date: Sun, 16 Dec 2018 05:15:47 +0400 Subject: [Histonet] IDH1 source In-Reply-To: References: Message-ID: <024E4517-F82D-49A0-894E-D58821937469@gmail.com> Good morning to all Richard Cartun: we use IDH1 on FFPE tissue. We purchase from Dianova, Germany. (I believe they are still the only suppliers worldwide). Purchased direct via their website. Vendors will also supply from the same source but at a higher price. Good luck Merry Christmas everyone! Anne Anne S van Binsbergen Supervisor, Histology Laboratory Pathology and Laboratory Medicine Sheikh Khalifa Medical City Abu Dhabi United Arab Emirates Sent from my iPhone > On Dec 15, 2018, at 10:00 PM, histonet-request at lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. IDH1 (Cartun, Richard) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 14 Dec 2018 20:38:38 +0000 > From: "Cartun, Richard" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] IDH1 > Message-ID: > <9215BD4B0BA1B44D962A71C758B68D2EAC0E2FD1 at HHCEXCHMB03.hhcsystem.org> > Content-Type: text/plain; charset="us-ascii" > > For those labs that are performing IDH1 IHC testing on FFPE tissue, which clone are you using and where do you get it? Thank you. > > Happy Holidays to everyone. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 (Office) > (860) 545-2204 (Fax) > Richard.cartun at hhchealth.org > > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 181, Issue 5 > **************************************** From edmartin26 at gmail.com Sun Dec 16 12:09:51 2018 From: edmartin26 at gmail.com (Eddie Martin) Date: Sun, 16 Dec 2018 13:09:51 -0500 Subject: [Histonet] IDH1 Message-ID: Hi Richard, We used Ventana H09 clone for IDH1 while I worked at Walter Reed NMMC. Sent from my iPhone > On Dec 14, 2018, at 3:38 PM, Cartun, Richard wrote: > > For those labs that are performing IDH1 IHC testing on FFPE tissue, which clone are you using and where do you get it? Thank you. > > Happy Holidays to everyone. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 (Office) > (860) 545-2204 (Fax) > Richard.cartun at hhchealth.org > > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > From john.garratt at ciqc.ca Sun Dec 16 15:37:28 2018 From: john.garratt at ciqc.ca (John Garratt) Date: Sun, 16 Dec 2018 21:37:28 +0000 Subject: [Histonet] IDH1 source In-Reply-To: <024E4517-F82D-49A0-894E-D58821937469@gmail.com> References: <024E4517-F82D-49A0-894E-D58821937469@gmail.com> Message-ID: If you check out the assessments page at www.ciqc.ca you will see EQA runs for IDH1. The protocols in the run reports will show the source of the Ab. John Sent from ProtonMail Mobile On Sat, Dec 15, 2018 at 5:15 PM, Anne van Binsbergen via Histonet wrote: > Good morning to all > Richard Cartun: we use IDH1 on FFPE tissue. > We purchase from Dianova, Germany. (I believe they are still the only suppliers worldwide). > Purchased direct via their website. > Vendors will also supply from the same source but at a higher price. > Good luck > Merry Christmas everyone! > Anne > > Anne S van Binsbergen > Supervisor, Histology Laboratory > Pathology and Laboratory Medicine > Sheikh Khalifa Medical City > Abu Dhabi > United Arab Emirates > > Sent from my iPhone > >> On Dec 15, 2018, at 10:00 PM, histonet-request at lists.utsouthwestern.edu wrote: >> >> Send Histonet mailing list submissions to >> histonet at lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-request at lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-owner at lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. IDH1 (Cartun, Richard) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Fri, 14 Dec 2018 20:38:38 +0000 >> From: "Cartun, Richard" >> To: "histonet at lists.utsouthwestern.edu" >> >> Subject: [Histonet] IDH1 >> Message-ID: >> <9215BD4B0BA1B44D962A71C758B68D2EAC0E2FD1 at HHCEXCHMB03.hhcsystem.org> >> Content-Type: text/plain; charset="us-ascii" >> >> For those labs that are performing IDH1 IHC testing on FFPE tissue, which clone are you using and where do you get it? Thank you. >> >> Happy Holidays to everyone. >> >> Richard >> >> Richard W. Cartun, MS, PhD >> Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory >> Director, Biospecimen Collection Programs >> Assistant Director, Anatomic Pathology >> Hartford Hospital >> 80 Seymour Street >> Hartford, CT 06102 >> (860) 972-1596 (Office) >> (860) 545-2204 (Fax) >> Richard.cartun at hhchealth.org >> >> >> This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. >> >> >> ------------------------------ >> >> Subject: Digest Footer >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ------------------------------ >> >> End of Histonet Digest, Vol 181, Issue 5 >> **************************************** > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Tue Dec 18 12:04:42 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 18 Dec 2018 13:04:42 -0500 Subject: [Histonet] Happy Holidays to You and Yours and Thank YOU!! Message-ID: <000001d496fc$26a85cc0$73f91640$@earthlink.net> Hi Histonetters, As 2018 comes to a close and we look forward to a New Year, I want to wish you a very Happy, Warm and Wonderful Holiday Season and a Promising, Prosperous and Terrific New Year. I really appreciate the leads that I get from you the subscribers to my histology bulletin and would like to say: Thank You!! I want to remind you about my referral program. I am offering a $250.00 dollar referral bonus for leads from you. I will pay you 250.00 dollars for every candidate that you refer to me who I place and every job lead you give me that I fill. Shoot me an e-mail or give me a call for more details. Happy Holidays to you and yours. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Happy Holidays! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From cls71877 at gmail.com Tue Dec 18 13:13:10 2018 From: cls71877 at gmail.com (Cristi Rigazio) Date: Tue, 18 Dec 2018 14:13:10 -0500 Subject: [Histonet] Beaker, Sendouts and ICD10 codes Message-ID: <47706BDB-8A7F-43D4-AF75-BF93C669CC30@gmail.com> Hi histo land! Anyone out there in the admin area using Beaker that can discuss workflows? I am curious how others are handling the new OPPS Medicare rule for molecular testing? Also, who is providing the ICD codes for your sendouts? Hope everyone is having a great day! Cristi Sent from my iPhone From pruegghm at hotmail.com Tue Dec 18 15:08:20 2018 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Tue, 18 Dec 2018 21:08:20 +0000 Subject: [Histonet] IDH1 source In-Reply-To: References: <024E4517-F82D-49A0-894E-D58821937469@gmail.com>, Message-ID: Hi Richard, How are you? Dianova is a really good ab supplier, I used their CD31 for animal research and it was excellent. Happy Holidays. Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: John Garratt Sent: Sunday, December 16, 2018 2:37 PM To: Anne van Binsbergen via Histonet Subject: Re: [Histonet] IDH1 source If you check out the assessments page at www.ciqc.ca you will see EQA runs for IDH1. The protocols in the run reports will show the source of the Ab. John Sent from ProtonMail Mobile On Sat, Dec 15, 2018 at 5:15 PM, Anne van Binsbergen via Histonet wrote: > Good morning to all > Richard Cartun: we use IDH1 on FFPE tissue. > We purchase from Dianova, Germany. (I believe they are still the only suppliers worldwide). > Purchased direct via their website. > Vendors will also supply from the same source but at a higher price. > Good luck > Merry Christmas everyone! > Anne > > Anne S van Binsbergen > Supervisor, Histology Laboratory > Pathology and Laboratory Medicine > Sheikh Khalifa Medical City > Abu Dhabi > United Arab Emirates > > Sent from my iPhone > >> On Dec 15, 2018, at 10:00 PM, histonet-request at lists.utsouthwestern.edu wrote: >> >> Send Histonet mailing list submissions to >> histonet at lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-request at lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-owner at lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. IDH1 (Cartun, Richard) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Fri, 14 Dec 2018 20:38:38 +0000 >> From: "Cartun, Richard" >> To: "histonet at lists.utsouthwestern.edu" >> >> Subject: [Histonet] IDH1 >> Message-ID: >> <9215BD4B0BA1B44D962A71C758B68D2EAC0E2FD1 at HHCEXCHMB03.hhcsystem.org> >> Content-Type: text/plain; charset="us-ascii" >> >> For those labs that are performing IDH1 IHC testing on FFPE tissue, which clone are you using and where do you get it? Thank you. >> >> Happy Holidays to everyone. >> >> Richard >> >> Richard W. Cartun, MS, PhD >> Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory >> Director, Biospecimen Collection Programs >> Assistant Director, Anatomic Pathology >> Hartford Hospital >> 80 Seymour Street >> Hartford, CT 06102 >> (860) 972-1596 (Office) >> (860) 545-2204 (Fax) >> Richard.cartun at hhchealth.org >> >> >> This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. >> >> >> ------------------------------ >> >> Subject: Digest Footer >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> ------------------------------ >> >> End of Histonet Digest, Vol 181, Issue 5 >> **************************************** > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at dpspa.com Wed Dec 19 07:59:23 2018 From: criley at dpspa.com (Charles Riley) Date: Wed, 19 Dec 2018 08:59:23 -0500 Subject: [Histonet] Breast lumpectomy fixation Message-ID: How does everyone gross and process your breast lumpectomy / mastectomy specimens? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From sandra.cheasty at wisc.edu Wed Dec 19 08:11:51 2018 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Wed, 19 Dec 2018 14:11:51 +0000 Subject: [Histonet] Used Lab Vision 720D Immunostainer Message-ID: Hello all, Does anyone know of a company who buys used Histology equipment? Our Lab Vision 720D Immunostainer still works fine, and had a full PM in July 2018. (We purchased a new IHC stainer that is better suited for research.) Cheers, Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From thomas6093 at yahoo.com Wed Dec 19 12:22:19 2018 From: thomas6093 at yahoo.com (Thomas Huynh) Date: Wed, 19 Dec 2018 18:22:19 +0000 (UTC) Subject: [Histonet] Histology equipment References: <1040823815.6827866.1545243739318.ref@mail.yahoo.com> Message-ID: <1040823815.6827866.1545243739318@mail.yahoo.com> Good morning everyone,Does anyone knows any company would rent out histology equipments? Embedding unit, microtome....ThanksThomas Sent from Yahoo Mail on Android From Richard.Cartun at hhchealth.org Wed Dec 19 13:17:21 2018 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Wed, 19 Dec 2018 19:17:21 +0000 Subject: [Histonet] Lyme Disease Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EAC0E3956@HHCEXCHMB03.hhcsystem.org> Is anyone doing PCR testing for Borrelia burgdorferi (Lyme Disease) from formalin-fixed, paraffin-embedded tissue? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From rsrichmond at gmail.com Wed Dec 19 17:38:17 2018 From: rsrichmond at gmail.com (Bob Richmond) Date: Wed, 19 Dec 2018 18:38:17 -0500 Subject: [Histonet] Breast lumpectomy fixation In-Reply-To: References: Message-ID: Charles Riley BS HT, HTL(ASCP)CM, Histopathology Coordinator/ Mohs (where?) asks: >>How does everyone gross and process your breast lumpectomy / mastectomy specimens?<< These are extremely complex and exacting procedures. It's your pathologist's job to do this, and your pathologist can't delegate any of the task to you without giving you a great deal of instruction. Don't try to charge into this unaided! Bob Richmond Samurai Pathologist, ret. Maryville TN From cls71877 at gmail.com Wed Dec 19 18:20:39 2018 From: cls71877 at gmail.com (Cristi Rigazio) Date: Wed, 19 Dec 2018 19:20:39 -0500 Subject: [Histonet] Breast lumpectomy fixation In-Reply-To: References: Message-ID: <85581908-6A8A-4D2B-8DC6-3AB807F42E79@gmail.com> PAs and pathologists are the only ones that gross them, we have six hours of formalin on the processing schedule to ensure we meet the minimum fixation time and Saturdays are rotated to ensure we never exceed 72 hours. Sent from my iPhone > On Dec 19, 2018, at 6:38 PM, Bob Richmond via Histonet wrote: > > Charles Riley BS HT, HTL(ASCP)CM, Histopathology Coordinator/ Mohs > (where?) asks: > >>> How does everyone gross and process your breast lumpectomy / mastectomy > specimens?<< > > These are extremely complex and exacting procedures. It's your > pathologist's job to do this, and your pathologist can't delegate any of > the task to you without giving you a great deal of instruction. Don't try > to charge into this unaided! > > Bob Richmond > Samurai Pathologist, ret. > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Douglas.Porter at sparrow.org Wed Dec 19 18:33:23 2018 From: Douglas.Porter at sparrow.org (Porter, Douglas) Date: Thu, 20 Dec 2018 00:33:23 +0000 Subject: [Histonet] [EXTERNAL] Re: Breast lumpectomy fixation In-Reply-To: <85581908-6A8A-4D2B-8DC6-3AB807F42E79@gmail.com> References: <85581908-6A8A-4D2B-8DC6-3AB807F42E79@gmail.com> Message-ID: Also, remember there is a minimum fixation time (cold ischemic) that is a requirement as well. It is 60 minutes. Some surgeons/surgery departments will not place the first breast specimen in formalin until they have removed the second breast when performing a double mastectomy. Help them to remember this requirement as they often forget when doing these procedures. Remember, the patient has entrusted us with their specimen and often their lives. We need to look out for them and be their advocates when it comes to handling of surgically removed tissue specimens. -----Original Message----- From: Cristi Rigazio via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, December 19, 2018 7:21 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] Breast lumpectomy fixation Warning: This email originated from outside of Sparrow. Do not click links or open attachments unless you recognize the sender and are expecting the message. ---------------------------------------------------------------------- PAs and pathologists are the only ones that gross them, we have six hours of formalin on the processing schedule to ensure we meet the minimum fixation time and Saturdays are rotated to ensure we never exceed 72 hours. Sent from my iPhone > On Dec 19, 2018, at 6:38 PM, Bob Richmond via Histonet wrote: > > Charles Riley BS HT, HTL(ASCP)CM, Histopathology Coordinator/ Mohs > (where?) asks: > >>> How does everyone gross and process your breast lumpectomy / >>> mastectomy > specimens?<< > > These are extremely complex and exacting procedures. It's your > pathologist's job to do this, and your pathologist can't delegate any > of the task to you without giving you a great deal of instruction. > Don't try to charge into this unaided! > > Bob Richmond > Samurai Pathologist, ret. > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthweste > rn.edu_mailman_listinfo_histonet&d=DwICAg&c=qgJBLQvENW4Kb9JcrSOXvj11QO > UUKGR5N2IUAtns1Jg&r=6pgpgKsLHvt-FitLISss8MQQkPawKdpRw8msCll96Ts&m=R6Xm > htRoUvvpoSQLF_WV79eZS2Z2FTnuXrLseHGVoW0&s=R-BDVbQ2hl8fZjD5eibEIrF-UwB8 > D_Oh1twkz5Rz0vI&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=qgJBLQvENW4Kb9JcrSOXvj11QOUUKGR5N2IUAtns1Jg&r=6pgpgKsLHvt-FitLISss8MQQkPawKdpRw8msCll96Ts&m=R6XmhtRoUvvpoSQLF_WV79eZS2Z2FTnuXrLseHGVoW0&s=R-BDVbQ2hl8fZjD5eibEIrF-UwB8D_Oh1twkz5Rz0vI&e= ________________________________ CONFIDENTIALITY NOTICE: This email communication may contain private, confidential, or legally privileged information intended for the sole use of the designated and/or duly authorized recipient(s). If you are not the intended recipient or have received this email in error, please notify the sender immediately by email and permanently delete all copies of this email including all attachments without reading them. If you are the intended recipient, secure the contents in a manner that conforms to all applicable state and/or federal requirements related to privacy and confidentiality of such information. ________________________________ From LRaff at uropartners.com Thu Dec 20 08:44:42 2018 From: LRaff at uropartners.com (Lester Raff MD) Date: Thu, 20 Dec 2018 14:44:42 +0000 Subject: [Histonet] Lab/Histology Blog link Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF11F8D542@COLOEXCH01.uropartners.local> Happy Holidays to all. A link to a blog all about those nasty bits of paraffin that drive me crazy. If you read, be sure to say hi in the comments and tell me you found us in the Histonet listserve. No spam, I promise!! Cut and paste address into your browser. Don't forget that last slash www.chicagonow.com/downsize-maybe/2018/12/the-pathologist-and-the-pea/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From brringer at SpringfieldClinic.com Thu Dec 20 10:21:47 2018 From: brringer at SpringfieldClinic.com (Ringer, Bradley) Date: Thu, 20 Dec 2018 16:21:47 +0000 Subject: [Histonet] 10% NBF Neutralization - Aldehyde test Message-ID: <30F02E79E90E7B46BA9D7B3FC705099F011832E735@E2K10DB.springfieldclinic.com> Does anyone currently neutralize formalin to dump down the sanitary system drain? We are currently using Formalex Green to neutralize our formalin for dumping. We have run into the issue of not being able to purchase aldehyde testing kits to verify the formalin has actually been neutralized before dumping. We have contacted the company, that in the past has sold us these aldehyde testing kits, and we were told they no longer sell them because histology labs are only checking the pH of the "neutralized formalin". After going back and forth a few times, the sales rep now tells us that we also need to be checking the aldehyde levels before dumping which is exactly what we have been trying to tell him. So, if anyone is neutralizing formalin to dump down the drain, are you checking the aldehyde levels and if so what product/test are you using? Thank you and Happy Holidays, Brad Brad Ringer, HTL (ASCP) Springfield Clinic Histology Manager 1025 S. 6th Street, Springfield, IL 62703 (217)528-7541 ext. 14078 This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From tony.auge at gmail.com Thu Dec 20 11:24:09 2018 From: tony.auge at gmail.com (Tony Auge) Date: Thu, 20 Dec 2018 09:24:09 -0800 Subject: [Histonet] 10% NBF Neutralization - Aldehyde test In-Reply-To: <30F02E79E90E7B46BA9D7B3FC705099F011832E735@E2K10DB.springfieldclinic.com> References: <30F02E79E90E7B46BA9D7B3FC705099F011832E735@E2K10DB.springfieldclinic.com> Message-ID: Hi Brad, I used the Neutrasafe Formzero Test Kit from statlab at my last job. It worked well. Happy Holidays, Tony On Thu, Dec 20, 2018 at 8:40 AM Ringer, Bradley via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Does anyone currently neutralize formalin to dump down the sanitary system > drain? We are currently using Formalex Green to neutralize our formalin for > dumping. We have run into the issue of not being able to purchase aldehyde > testing kits to verify the formalin has actually been neutralized before > dumping. We have contacted the company, that in the past has sold us these > aldehyde testing kits, and we were told they no longer sell them because > histology labs are only checking the pH of the "neutralized formalin". > After going back and forth a few times, the sales rep now tells us that we > also need to be checking the aldehyde levels before dumping which is > exactly what we have been trying to tell him. So, if anyone is > neutralizing formalin to dump down the drain, are you checking the aldehyde > levels and if so what product/test are you using? > > Thank you and Happy Holidays, > > Brad > > Brad Ringer, HTL (ASCP) > Springfield Clinic Histology Manager > 1025 S. 6th Street, Springfield, IL 62703 > (217)528-7541 ext. 14078 > > > This electronic message contains information from Springfield Clinic, LLP > that may be confidential, privileged, and/or sensitive. This information is > intended for the use of the individual(s) or entity(ies) named above. If > you are not the intended recipient, be aware that disclosure, copying, > distribution, or action taken on the contents of this information is > strictly prohibited. If you have received this electronic message in error, > please notify the sender immediately, by electronic mail, so that > arrangements may be made for the retrieval of this electronic message. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tony Auge HTL (ASCP)CM QIHC Cell: (651) 373-4768 Email: tony.auge at gmail.com From ronald.kusters at pfmmedical.com Fri Dec 21 01:19:51 2018 From: ronald.kusters at pfmmedical.com (Kusters, Ronald) Date: Fri, 21 Dec 2018 07:19:51 +0000 Subject: [Histonet] Lab/Histology Blog link In-Reply-To: <6347C6D2B080534F9B5C2B08436DCFAF11F8D542@COLOEXCH01.uropartners.local> References: <6347C6D2B080534F9B5C2B08436DCFAF11F8D542@COLOEXCH01.uropartners.local> Message-ID: Hi, The message is 'Unfortunately, our website is currently unavailable in most European countries. We are engaged on the issue and committed to looking at options that support our full range of digital offerings to the EU market. We continue to identify technical compliance solutions that will provide all readers with our award-winning journalism.' News is obviously only for US... Happy holidays. Ronald Mit freundlichen Gr??en/Kind Regards Ronald Kusters Global Sales Manager Feather Brand Histotechnology pfm medical ag Wankelstra?e 60 50996 K?ln, Germany T +49 (2236) 9641 660 F +49 (2236) 9641 99 660 M +49 (171) 9144343 -----Original Message----- From: Lester Raff MD [mailto:LRaff at uropartners.com] Sent: 20 December 2018 15:45 To: 'Histonet (histonet at lists.utsouthwestern.edu)' Subject: [Histonet] Lab/Histology Blog link Happy Holidays to all. A link to a blog all about those nasty bits of paraffin that drive me crazy. If you read, be sure to say hi in the comments and tell me you found us in the Histonet listserve. No spam, I promise!! Cut and paste address into your browser. Don't forget that last slash www.chicagonow.com/downsize-maybe/2018/12/the-pathologist-and-the-pea/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From patpxs at gmail.com Fri Dec 21 13:50:23 2018 From: patpxs at gmail.com (P Sicurello) Date: Fri, 21 Dec 2018 11:50:23 -0800 Subject: [Histonet] GMS Failures on Ventana BMSS Message-ID: Happy Solstice, Happy Friday, We are having a lot of GMS staining run failures on our BenchMark Special Stainers. The problem is not predictable. It's not slide position related because one day position 2 (example) works, the next run it doesn't. This is only happening with the GMS stains. Has anyone out there suffered a similar fate? We have tried just about everything to fix the problem to no avail. If you had this happen to you and you were able to fix the problem, please send me your notes. We bought these machines to make things easier - not make us pull our hair out. Thank you and have safe and wonderful Holidays. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From patpxs at gmail.com Mon Dec 24 11:31:37 2018 From: patpxs at gmail.com (P Sicurello) Date: Mon, 24 Dec 2018 09:31:37 -0800 Subject: [Histonet] Ventana BenchMark Special Stainer Message-ID: Happy Christmas Eve, I sit here reading several replies about the failure of GMS stains. There is one theme that pretty much everyone has mentioned, and I can say we agree with them, Ventana/Roche blames the user and does nothing to help correct this problem. We have heard the same as everyone else: User error, blame the wash buffer - make fresh every day, etc, blame everyone and everything except themselves. We have 3 of the Special Stainers, one of them we have used almost non-stop since November 2015. It had a problem with Trichrome stains over two years ago, which was fixed by balancing the vortex mixers (they told us it was user error then too). Now that the machine has been relocated and the software has been upgraded, a machine that rarely, if ever, had a GMS stain fail now fails with regularity. Ventana/Roche needs to realize that they have a serious problem and not continue to sweep it under the rug. It's not the wash buffer, it's not the user, it's something else and they do not want to own up to it or help come up with a solution (no pun intended). We have spent a lot of money on these machines, all the kits they had us throw out because they blamed the kit, all the wash buffer we dumped because they told us we had to make fresh every day (we use these machines so much that the wash buffer does not sit around and get icky), and their response is silence. Sorry about the rant, but this is bugging me and I had to vent. Have a safe a wonderful Holiday Season- whatever you celebrate -or don't celebrate. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From tbraud at holyredeemer.com Mon Dec 24 12:24:00 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 24 Dec 2018 18:24:00 +0000 Subject: [Histonet] Roche/Ventana rant Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF8E65D@HRHEX02-HOS.holyredeemer.local> My sympathies. I wish we had never bought the Ventana Ultra. The constant failures of the dispensers has caused such a headache, and the documentation needed before they will even agree to replace one reagent, and then the fight to obtain a credit for the entire test, not to mention wasted tech time and valuable patient tissue. Roche - I hope you are watching this. Your customer service is terrible and you do nothing but try to cover up problems. Still waiting on my refund from the H.Pylorii recall - 72 tests that had to be repeated!!! Another unhappy customer. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Monday, December 24, 2018 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 181, Issue 12 CAUTION : External Email Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Ventana BenchMark Special Stainer (P Sicurello) ---------------------------------------------------------------------- Message: 1 Date: Mon, 24 Dec 2018 09:31:37 -0800 From: P Sicurello To: HistoNet Subject: [Histonet] Ventana BenchMark Special Stainer Message-ID: Content-Type: text/plain; charset="UTF-8" Happy Christmas Eve, I sit here reading several replies about the failure of GMS stains. There is one theme that pretty much everyone has mentioned, and I can say we agree with them, Ventana/Roche blames the user and does nothing to help correct this problem. We have heard the same as everyone else: User error, blame the wash buffer - make fresh every day, etc, blame everyone and everything except themselves. We have 3 of the Special Stainers, one of them we have used almost non-stop since November 2015. It had a problem with Trichrome stains over two years ago, which was fixed by balancing the vortex mixers (they told us it was user error then too). Now that the machine has been relocated and the software has been upgraded, a machine that rarely, if ever, had a GMS stain fail now fails with regularity. Ventana/Roche needs to realize that they have a serious problem and not continue to sweep it under the rug. It's not the wash buffer, it's not the user, it's something else and they do not want to own up to it or help come up with a solution (no pun intended). We have spent a lot of money on these machines, all the kits they had us throw out because they blamed the kit, all the wash buffer we dumped because they told us we had to make fresh every day (we use these machines so much that the wash buffer does not sit around and get icky), and their response is silence. Sorry about the rant, but this is bugging me and I had to vent. Have a safe a wonderful Holiday Season- whatever you celebrate -or don't celebrate. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 181, Issue 12 ***************************************** From Desiree.Ledee at alegent.org Mon Dec 24 16:48:30 2018 From: Desiree.Ledee at alegent.org (Ledee,Desiree Y) Date: Mon, 24 Dec 2018 22:48:30 +0000 Subject: [Histonet] Ventana BenchMark Special Stainer In-Reply-To: References: Message-ID: We have the benchmark specials stainers and don?t have any issues with the GMS. We make our wash around every 4-6 weeks and use DI water. We also decon the wash bulk container once month. Decons are performed quarterly on instruments Get Outlook for iOS ________________________________ From: P Sicurello via Histonet Sent: Monday, December 24, 2018 11:32 AM To: HistoNet Subject: [Histonet] Ventana BenchMark Special Stainer CAUTION: This email is not from a CHI source. Only click links or open attachments you know are safe. Please send as an attachment all spam/phishing and unusual emails to spam at catholichealth.net. ...................................................................... Happy Christmas Eve, I sit here reading several replies about the failure of GMS stains. There is one theme that pretty much everyone has mentioned, and I can say we agree with them, Ventana/Roche blames the user and does nothing to help correct this problem. We have heard the same as everyone else: User error, blame the wash buffer - make fresh every day, etc, blame everyone and everything except themselves. We have 3 of the Special Stainers, one of them we have used almost non-stop since November 2015. It had a problem with Trichrome stains over two years ago, which was fixed by balancing the vortex mixers (they told us it was user error then too). Now that the machine has been relocated and the software has been upgraded, a machine that rarely, if ever, had a GMS stain fail now fails with regularity. Ventana/Roche needs to realize that they have a serious problem and not continue to sweep it under the rug. It's not the wash buffer, it's not the user, it's something else and they do not want to own up to it or help come up with a solution (no pun intended). We have spent a lot of money on these machines, all the kits they had us throw out because they blamed the kit, all the wash buffer we dumped because they told us we had to make fresh every day (we use these machines so much that the wash buffer does not sit around and get icky), and their response is silence. Sorry about the rant, but this is bugging me and I had to vent. Have a safe a wonderful Holiday Season- whatever you celebrate -or don't celebrate. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 (P): 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=YFhW2PYwN3hsZhoCqLOPHsIEIPQ6qDXkZ40AlEYUG9c&r=T2dmrnFWSvsDOLewkVlIOSwd73D0unldueOGYSGHi6w&m=KpFwUCn4ICqp5yQjaPlq0DAfc9nZfrMycc6pJSiWKk4&s=swmM0t8kSh2B9z9HvROu6EBin9HI9f61UpgqEJ1KpB4&e= ________________________________ The information contained in this communication, including attachments, is confidential and private and intended only for the use of the addressees. Unauthorized use, disclosure, distribution or copying is strictly prohibited and may be unlawful. If you received this communication in error, please inform us of the erroneous delivery by return e-mail message from your computer. Additionally, although all attachments have been scanned at the source for viruses, the recipient should check any attachments for the presence of viruses before opening. CHI Health accepts no liability for any damage caused by any virus transmitted by this e-mail. Thank you for your cooperation. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From CDavis at che-east.org Wed Dec 26 06:54:48 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Wed, 26 Dec 2018 12:54:48 +0000 Subject: [Histonet] Input Request please Message-ID: Good morning Histonet, I am told our contract with Roche for the Ventana instruments will be up in March. Like many of you we have been having significant issues with our special strainers especially the GMS and are being told the same things you are as well as it's because our instrument is too old (2009) and we need to purchase a replacement. I would like to present our laboratory director with input from different sources so we can make an informed decision. Please take a moment and answer the following questions and if you do not want the whole Histonet to see your honest answers please feel free to respond with a direct email to me at CDavis at che-east.org. Thank you in advance for your time and your input Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From CDavis at che-east.org Wed Dec 26 07:00:16 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Wed, 26 Dec 2018 13:00:16 +0000 Subject: [Histonet] Input Request please In-Reply-To: References: Message-ID: <85b7d23c72ae45b1b0af1b491ce4f62c@che-east.org> Good Morning Histonet, I am told our contract with Roche for the Ventana instruments will be up in March. Like many of you we have been having significant issues with our special strainers especially the GMS and are being told the same things you are as well as it's because our instrument is too old (2009) and we need to purchase a replacement. I would like to present our laboratory director with input from different sources so we can make an informed decision. Please take a moment and answer the following questions and if you do not want the whole Histonet to see your honest answers please feel free to respond with a direct email to me at CDavis at che-east.org. Thank you in advance for your time and your input. 1) Who is your vendor for your automated IHC and SS instrument? 2) What size is your lab or approximate IHC/SS volume? (IHC/SS everyday/weekly/batches?) 3) If you could change anything about your instrument(s) what would that be? Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From ewj at pigs.ag Wed Dec 26 07:24:29 2018 From: ewj at pigs.ag (ewj) Date: Wed, 26 Dec 2018 21:24:29 +0800 Subject: [Histonet] microscope Message-ID: <1545828980717.rs40fzsvz340zuxwmzju2h1h@android.mail.163.com> we are going to buy a new microscope for standard histopath. ?we wont be doing any fluorescent microscopy. ?we will likely take quite a few pictures. i am considering a Nikon Ci base and planachromat lenses. ?does anyone have experience with Nikon scopes and any comments? Dr. E. Wayne Johnson Enable AgTech Beijing From amarr at med.umich.edu Wed Dec 26 08:20:03 2018 From: amarr at med.umich.edu (Oler, April) Date: Wed, 26 Dec 2018 14:20:03 +0000 Subject: [Histonet] Brachyury Message-ID: <824b977400374d0f94be13f7913945fd@med.umich.edu> Good morning Histonet, Has anyone had luck finding a Brachyury antibody that works well? After the Santa Cruz (H-210) was discontinued, I've been unable to find an antibody to replace the H-210. I've tried two antibodies (Santa Cruz D-10 and BioSB X1A02), but I was unable to validate either of them due to inconsistent staining results. Thank you in advance for any information you can provide. April Oler, HT Lead Tech IHC/DIF/Muscle Michigan Medicine University of Michigan ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues From bcooper at chla.usc.edu Wed Dec 26 11:43:48 2018 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 26 Dec 2018 17:43:48 +0000 Subject: [Histonet] Brachyury In-Reply-To: <824b977400374d0f94be13f7913945fd@med.umich.edu> References: <824b977400374d0f94be13f7913945fd@med.umich.edu> Message-ID: <615140f20ec846a3a4f5914ab02966eb@chla.usc.edu> We use the mouse monoclonal (1H9A2) from AbCam. Works like a charm. https://www.abcam.com/brachyury--bry-antibody-1h9a2-ab140661.html Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcooper at chla.usc.edu -----Original Message----- From: Oler, April via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, December 26, 2018 6:20 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] Brachyury (EXTERNAL EMAIL) Good morning Histonet, Has anyone had luck finding a Brachyury antibody that works well? After the Santa Cruz (H-210) was discontinued, I've been unable to find an antibody to replace the H-210. I've tried two antibodies (Santa Cruz D-10 and BioSB X1A02), but I was unable to validate either of them due to inconsistent staining results. Thank you in advance for any information you can provide. April Oler, HT Lead Tech IHC/DIF/Muscle Michigan Medicine University of Michigan ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From relia1 at earthlink.net Thu Dec 27 12:14:34 2018 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 27 Dec 2018 13:14:34 -0500 Subject: [Histonet] Let's Get The Ball Rolling Before "The Big Ball" Drops it's almost 2019!! Message-ID: <000b01d49e10$055949b0$100bdd10$@earthlink.net> Hello Histonetters, It is hard to believe that 2018 is almost over. 2019 is just a few days away. Looking for a new job in the New Year? Planning a job change after the Holidays? Considering making a move in 2019? Are you a Histo Tech looking for? 1. Higher pay? 2. Better hours? 3. Better situation in general? Are you a Histo Tech who wants to relocate to a new area? Are you a Traveler considering transitioning into a permanent position? Are you a Histo Tech looking for an opportunity to advance into management? Histonetters! If you answered YES to any of the above questions We Need to Chat!! Let?s Get The Ball Rolling!!! Just shoot me an email with the time you would like to talk and the phone number you would like for me to use. If you would like to contact me directly I can be reached toll free at 866-607-3542, email: relia1 at earthlink.net or cell/text 407-353-5070. OR Feel free to kick the idea around with family and friends and shoot me an email next week if you would like to chat after the New Year Holiday. If you know of anyone else who might be interested in subscribing to RELIA?s Histology Careers Bulletin please feel free to pass this along to them. And if you refer someone and I place them I will pay you a referral fee. I Have Exciting Opportunities In These Areas: ? Texas ? North Carolina ? Tennessee ? Massachusetts ? Florida ? Alabama ? Wisconsin ? Ohio All of the opportunities that I represent are full time permanent positions with premier facilities throughout the United States. My clients offer competitive salaries, great benefits and in most cases relocation assistance and or sign on bonuses! Remember it never hurts to look!!! Don?t see any positions that catch your eye? No worries! I am getting new positions daily I could get your next job tomorrow so if you are looking at all Call Me!! Thanks, Pam 866-60 RELIA (866-607-3542) I know there are a lot of recruiters out there right now contacting you and your friends. RELIA is the only nationwide recruiting firm specializing in the permanent placement of histology professionals. Remember here at RELIA we work as your confidential advocate to help you make the move that is right for you when the time is right for you. Happy New Year!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Linda.Margraf at cookchildrens.org Thu Dec 27 15:30:18 2018 From: Linda.Margraf at cookchildrens.org (Linda Margraf) Date: Thu, 27 Dec 2018 21:30:18 +0000 Subject: [Histonet] Histotech needed for Greenville TX lab Message-ID: <61f54f2fdf85416793c5393b68c528d7@MBX10.CCHCS.LDAP> Here is a job opening in Greenville, TX I was asked to put on the Histonet list...... Pathology Associates of Greenville Texas is seeking to hire a Full-time Histotechnician for a 4 Pathologists CAP accredited Laboratory. We are a fully automated Histology Laboratory The successful candidate should be familiar with: Embedding, Microtomy, Leica H& E stainer, Leica Bond Immunohistochemistry and be able to perform Special Stains. Understands administrative duties of the Histology Laboratory. Willing to learn additional Molecular related assays specifically performed and help with Frozen sections . Process small routine biopsies. We have an excellent benefit package . Salary: $35,000.00 to $41,000.00 /year, depending on experience . Interested; contact mebrahim at pathologyassociatestx.com and cwest at pathologyassociatestx.com From Richard.Cartun at hhchealth.org Fri Dec 28 17:08:29 2018 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 28 Dec 2018 23:08:29 +0000 Subject: [Histonet] IDH1 Antibody Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EAC0E4E8B@HHCEXCHMB03.hhcsystem.org> If you are doing IHC testing for IDH1 here in the United States, do you get the antibody (clone HO9) directly from Dianova in Germany or is there a US distributor? I did try HistoBiotech, but they no longer carry Dianova antibodies. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From amarr at med.umich.edu Sat Dec 29 11:03:58 2018 From: amarr at med.umich.edu (Oler, April) Date: Sat, 29 Dec 2018 17:03:58 +0000 Subject: [Histonet] IDH1 Antibody In-Reply-To: <9215BD4B0BA1B44D962A71C758B68D2EAC0E4E8B@HHCEXCHMB03.hhcsystem.org> References: <9215BD4B0BA1B44D962A71C758B68D2EAC0E4E8B@HHCEXCHMB03.hhcsystem.org> Message-ID: <2af59418b83540f3a1f85918ea9dc8b3@med.umich.edu> Hi Richard, IDH1 is now distributed by ARP. https://www.arp1.com/products/antibodies/monoclonal-antibodies.html/anti-idh1-r132h-monoclonal-antibody-ready-to-use.html April Oler Lead Tech IHC/DIF/Muscles Michigan Medicine University of Michigan ________________________________ From: Cartun, Richard via Histonet Sent: Friday, December 28, 2018 6:08:29 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IDH1 Antibody External Email - Use Caution If you are doing IHC testing for IDH1 here in the United States, do you get the antibody (clone HO9) directly from Dianova in Germany or is there a US distributor? I did try HistoBiotech, but they no longer carry Dianova antibodies. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) Richard.cartun at hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues From portera at msu.edu Sat Dec 29 13:16:08 2018 From: portera at msu.edu (Porter, Amy) Date: Sat, 29 Dec 2018 19:16:08 +0000 Subject: [Histonet] IDH1 Antibody In-Reply-To: <2af59418b83540f3a1f85918ea9dc8b3@med.umich.edu> References: <9215BD4B0BA1B44D962A71C758B68D2EAC0E4E8B@HHCEXCHMB03.hhcsystem.org>, <2af59418b83540f3a1f85918ea9dc8b3@med.umich.edu> Message-ID: Excellent!!! Sent from my iPhone > On Dec 29, 2018, at 12:04 PM, Oler, April via Histonet wrote: > > Hi Richard, > > > IDH1 is now distributed by ARP. > > https://urldefense.proofpoint.com/v2/url?u=https-3A__www.arp1.com_products_antibodies_monoclonal-2Dantibodies.html_anti-2Didh1-2Dr132h-2Dmonoclonal-2Dantibody-2Dready-2Dto-2Duse.html&d=DwICAg&c=nE__W8dFE-shTxStwXtp0A&r=CqfpxVox3wg0MOYeDRPiDg&m=pXWGlHMCxF7-l4zy-xdnOLPBuliYlPUuEjbJjtYimmE&s=fpQH394Vool18mtG0_M-JUYgsVLvwt-00wCEZgaIIOw&e= > > > April Oler > > Lead Tech IHC/DIF/Muscles > > Michigan Medicine > > University of Michigan > > ________________________________ > From: Cartun, Richard via Histonet > Sent: Friday, December 28, 2018 6:08:29 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] IDH1 Antibody > > External Email - Use Caution > > If you are doing IHC testing for IDH1 here in the United States, do you get the antibody (clone HO9) directly from Dianova in Germany or is there a US distributor? I did try HistoBiotech, but they no longer carry Dianova antibodies. Thank you. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 (Office) > (860) 545-2204 (Fax) > Richard.cartun at hhchealth.org > > > This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=nE__W8dFE-shTxStwXtp0A&r=CqfpxVox3wg0MOYeDRPiDg&m=pXWGlHMCxF7-l4zy-xdnOLPBuliYlPUuEjbJjtYimmE&s=RA4mvOvsEzYcMtoOYP-cLn7kDNxRI-gVx10zaq8iPRo&e= > > ********************************************************** > Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=nE__W8dFE-shTxStwXtp0A&r=CqfpxVox3wg0MOYeDRPiDg&m=pXWGlHMCxF7-l4zy-xdnOLPBuliYlPUuEjbJjtYimmE&s=RA4mvOvsEzYcMtoOYP-cLn7kDNxRI-gVx10zaq8iPRo&e= From mgiorgi at incdx.com Mon Dec 31 12:39:39 2018 From: mgiorgi at incdx.com (Miranda Giorgi) Date: Mon, 31 Dec 2018 18:39:39 +0000 Subject: [Histonet] Multi-Tumor Controls for IHC Validation Message-ID: Hello, Does anyone use multi tumor tissue controls for their IHC validations? If so, what do you use in them? We have been staining multi-tumor controls with our validation sets on top of the 10 positive and 10 negative cases to test specificity, but we are wondering if this is necessary. Plus, the blocks are sometimes difficult to maintain. I'd love to hear what other labs are doing. Thank you, Miranda Giorgi, HTL (ASCP)cm Histology Manager Incyte Diagnostics 509-892-2744 This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700.