From mfb.encarnacion at gmail.com Wed Aug 1 09:16:15 2018 From: mfb.encarnacion at gmail.com (Mary Faith Encarnacion) Date: Wed, 1 Aug 2018 10:16:15 -0400 Subject: [Histonet] =?utf-8?q?Pathologists=E2=80=99_Assistant_Competencies?= Message-ID: <9F206C5D-6FCA-46A2-A312-D6B76CB4CB6E@gmail.com> Good morning HistoNet, I?m hoping to find competencies for on-the-job trained pathologists? assistants and pathologists? assistants that are certified or completed an accredited program. Are there certain expectations to gross x amount of specimens in a certain amount of time? Any help would be appreciated! Thank you, Mary Faith E. Philadelphia VA Sent from my iPhone From mim9060 at nyp.org Wed Aug 1 13:00:53 2018 From: mim9060 at nyp.org (Michele Maimone-Schoen) Date: Wed, 1 Aug 2018 18:00:53 +0000 Subject: [Histonet] Pathologist Assistants Competency/Benchmarks Message-ID: We measure the following depending on the number of PathA team members available and case mix volume: Cases dictated / month: = 500-625 Cassette count / month = 1000-1500 -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Wednesday, August 01, 2018 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 177, Issue 1 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=apLCJo22jkVRpFivmRGGHnRn85FoLi_g9mEBSlVKwRY&r=glcV8HADLxkuv0pL9qAWXQ&m=F-Y2QRP7zoxyhfyrC8xaGzFcbF2gcNNZPxmDrIPbbeM&s=kIl6_L0O0uMuCMTG2wSz_9knUYsHxByH3F5uKMs1h1Y&e= or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Pathologists? Assistant Competencies (Mary Faith Encarnacion) ---------------------------------------------------------------------- Message: 1 Date: Wed, 1 Aug 2018 10:16:15 -0400 From: Mary Faith Encarnacion To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Pathologists? Assistant Competencies Message-ID: <9F206C5D-6FCA-46A2-A312-D6B76CB4CB6E at gmail.com> Content-Type: text/plain; charset=utf-8 Good morning HistoNet, I?m hoping to find competencies for on-the-job trained pathologists? assistants and pathologists? assistants that are certified or completed an accredited program. Are there certain expectations to gross x amount of specimens in a certain amount of time? Any help would be appreciated! Thank you, Mary Faith E. Philadelphia VA Sent from my iPhone ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=apLCJo22jkVRpFivmRGGHnRn85FoLi_g9mEBSlVKwRY&r=glcV8HADLxkuv0pL9qAWXQ&m=F-Y2QRP7zoxyhfyrC8xaGzFcbF2gcNNZPxmDrIPbbeM&s=kIl6_L0O0uMuCMTG2wSz_9knUYsHxByH3F5uKMs1h1Y&e= ------------------------------ End of Histonet Digest, Vol 177, Issue 1 **************************************** ---------------------------------------------------------------------- This electronic message is intended to be for the use only of the named recipient, and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. Thank you. code:d34y From naira.margaryan at hsc.wvu.edu Fri Aug 3 08:33:01 2018 From: naira.margaryan at hsc.wvu.edu (Margaryan, Naira) Date: Fri, 3 Aug 2018 13:33:01 +0000 Subject: [Histonet] Coverslipper Message-ID: Happy Friday, We would like to sell our slightly used but in excellent condition Dako Coverslipper which was purchased in 2010 $10K (purchase price was $ 25K). The instrument can handle up to 600 slides per hour making it one of the fastest on the market. In addition to the flexibility of the Coverslipper it is easy and straightforward to operate and cleaning and maintenance is simple to do. It is small enough to fit into fume cabinets, easy to move around and accepts a variety of commercial mounting media. Will take any offer, Naira From criley at dpspa.com Fri Aug 3 13:18:50 2018 From: criley at dpspa.com (Charles Riley) Date: Fri, 3 Aug 2018 14:18:50 -0400 Subject: [Histonet] Saukara Prisma Plus Message-ID: Does anyone out there use this for their routine H&E stains as well as GYN and Non GYN staining? I am trying to figure out staining protocols. If anyone would be willing to share their procedures as well as who you purchase your supplies from it would be greatly appreciated. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From VKurth at uwhealth.org Mon Aug 6 09:36:16 2018 From: VKurth at uwhealth.org (Kurth Virginia L.) Date: Mon, 6 Aug 2018 14:36:16 +0000 Subject: [Histonet] PROX1 Message-ID: Hello Does anyone send out for Prox1? Thanks. Ginny UW-madison From lmarie08 at uga.edu Mon Aug 6 10:27:14 2018 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Mon, 6 Aug 2018 15:27:14 +0000 Subject: [Histonet] feathers Message-ID: Hello Histoland, A researcher brought us a group of poultry feather shafts to embed and section for routine and IHC staining. Has anyone out there had any experience with this that could share some advice on getting some successful sections? So far we have tried facing the blocks and then applying "Nair for Men" for ten minutes to the block surface, followed by soaking the blocks for some time before attempting sectioning but still we have not had any luck. The sections turn out with large holes and blow apart on the water bath. Any tips would be appreciated, thanks! Lauren From jriggleman at globusmedical.com Mon Aug 6 12:09:52 2018 From: jriggleman at globusmedical.com (Jessica Riggleman) Date: Mon, 6 Aug 2018 17:09:52 +0000 Subject: [Histonet] Histonet Digest, Vol 177, Issue 5 In-Reply-To: References: Message-ID: We let our tough blocks soak in Downy fabric softener face down (depending, 10-30 minutes) and then try to section, sometimes use ice, then coat our slides in egg whites if they are tough to adhere. _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Monday, August 06, 2018 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 177, Issue 5 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From relia1 at earthlink.net Tue Aug 7 09:54:18 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 7 Aug 2018 10:54:18 -0400 Subject: [Histonet] Relia Hot Histology Job Alert - 8-07- 2018 Exciting New Opportunities as the Summer Flies By! Message-ID: <000001d42e5e$84c259c0$8e470d40$@earthlink.net> Hello Histonetters How are you doing today? Is it me or is this Summer FLYING by? I am sending this special alert out because I am ending the summer just like I started it with some of the best job opportunities out there. I am especially excited about these openings because they are with several of my best clients. Why do I refer to them as best clients? Well for a variety of reasons: ? Location, ? The rest of the team, ? The fantastic leadership, ? Excellent benefits, ? Generous relocation ? And most importantly I like a client that has openings due to growth. RELIA Spotlight Opportunities: Get off the bench and use your histo skills in a new and exciting way: Histology Sales Support Rep ? San Francisco,CA Histology Sales Support Rep ? San Diego, CA Here is a list of the positions that I am most excited about: ? Fayetteville, NC Histotechnician IHC exper a plus. ? Atlanta, GA Grossing Histotech CLIA Qualified! ? Kansas City, MO Histotechnician Learn or use your IHC! ? San Francisco, CA Sales Support Rep. Exciting Brand New CO!! ? San Diego, CA Sales Support Rep. Exciting Brand New CO!! ? Modesto, CA Histology Tech Great environment to work in! ? Chattanooga, TN Histology Tech IHC required. ? Chicago area Histology Tech Exciting Fast paced Lab!! All of these are full time permanent positions and most of my clients offer relocation assistance and or a sign on bonus! Most importantly they can?t wait to talk to YOU!! If you or anyone you know is interested in more details, I can be reached toll free at 866-607-3542 or on my cell at 407-353-5070 or at relia1 at earthlink.net. If you are starting to save up your Christmas money remember, $$$$ I pay a referral fee to you if I place someone that you refer to me. Thanks and have a great day!! Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From tmcampbe at fmh.org Wed Aug 8 06:33:35 2018 From: tmcampbe at fmh.org (Campbell, Tasha M.) Date: Wed, 8 Aug 2018 11:33:35 +0000 Subject: [Histonet] Iron Stain Message-ID: Is nuclear fast red the only counter stain for Prussian blue stain? I have a Masson's trichrome kit and was wondering if the Bierbech Scarlet could be a counterstain. I won't be doing the iron stain very often at all so I am trying to keep dry reagents on hand to make up as needed so they do not expire so quickly. Thanks! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 From kjgada at gmail.com Wed Aug 8 10:27:17 2018 From: kjgada at gmail.com (Komal Gada) Date: Wed, 8 Aug 2018 11:27:17 -0400 Subject: [Histonet] Request for Partnership for HT Students Message-ID: Hello Histo-netters, I am writing once again on the behalf of Keiser University's Associates in Histotechnology program. The education of future histotechs through this program consists of eight months of classroom / lab instruction followed by two months of a clinical rotatation (externship). Currently, we partner with many facilities in Central Florida to ensure our students receive the best possible experience and graduate as competent Histotechs. I am reaching out to offer this opportunity to partner with Keiser University as a possible externship site for anyone interested. In becoming an affiliate site. You would agree to accept a Histotechnology student that meets your approval, and host them through their externship. In return, you will gain full time assistance for eight weeks, at *no cost to you* (other than a little guidance, as needed). All in all, it is a win-win situation all around. Many facilities have found that the externship program is an excellent way to screen potential new employees. There is an opportunity to freshly mold the student and train him or her in a way that is specific to the facility, while gaining assistance for two months at no cost. Employment is never implied, though it is always an option, should the student be the perfect fit for your facility. All in all, the program is a great way to determine if an individual will work well with your staff before making any kind of commitment. If you are interested in having *Histotechnology students* volunteer in your facility, would like more information, or have any questions, please email me, and I will respond as quickly as possible. While most of the students prefer Florida sites, we do have students who request to complete externships throughout the nation.We have partnered with clinical sites in Georgia, Maryland, New York, Illinois and even the Bahamas! Currently, we have requests from students to complete their externships in the Los Angeles, CA area and Tampa, FL. My email is kjgada at gmail.com or kgada at keiseruniversity.edu. I look forward to hearing from you, Komal *Komal Gada, BS, HT/HTL(ASCP), QIHC* *Histotechnology Program Director* From rsrichmond at gmail.com Wed Aug 8 12:33:34 2018 From: rsrichmond at gmail.com (Bob Richmond) Date: Wed, 8 Aug 2018 13:33:34 -0400 Subject: [Histonet] Iron Stain Message-ID: Tasha Campbell, B.S.,HTL(ASCP), at Frederick Gastroenterology Associates in Frederick, Maryland asks: >>Is nuclear fast red the only counter stain for the Prussian blue stain? I have a Masson's trichrome kit and was wondering if [Biebrich] scarlet could be [used as] a counterstain. I won't be doing the iron stain very often at all so I am trying to keep dry reagents on hand to make up as needed so they do not expire so quickly.<< This old pathologist never found Biebrich scarlet or the various fuchsins to be satisfactory substitutes for nuclear fast red - they're too dense, and they don't stain nuclei specifically. Nuclear fast red (Kernechtrot, C.I. 60760) is a dye with problems - poor shelf life both dry and in solution, and it will probably go out of manufacture eventually. It's used as an aluminum lake for staining nuclei. I'm not sure what would serve as an alternative. I'd like to know more about brazilin (particularly about Anatech's Brazilliant), a catechol dye similar to hematoxylin, with good specificity for nuclei. Bob Richmond Samurai Pathologist Maryville TN From llewllew at shaw.ca Wed Aug 8 12:42:05 2018 From: llewllew at shaw.ca (Bryan Llewellyn) Date: Wed, 8 Aug 2018 10:42:05 -0700 Subject: [Histonet] Iron Stain In-Reply-To: References: Message-ID: 1% neutral red in 0.01% acetic acid (1 mL of 1% per 100 mL stain) works, as does 1% safranin. Both are stable, although should be filtered from time to time. Stain 1 minute, water rinse, ethanol, xylene and mount should work. I had one pathologist who preferred a very light progressive H&E. She said she was interested in increased amounts of iron and normal amounts not being glaringly obvious didn't bother her. Bryan Llewellyn Bob Richmond via Histonet wrote: > Tasha Campbell, B.S.,HTL(ASCP), at Frederick Gastroenterology Associates in > Frederick, Maryland asks: > >>> Is nuclear fast red the only counter stain for the Prussian blue stain? I > have a Masson's trichrome kit and was wondering if [Biebrich] scarlet could > be [used as] a counterstain. I won't be doing the iron stain very often at > all so I am trying to keep dry reagents on hand to make up as needed so > they do not expire so quickly.<< > > This old pathologist never found Biebrich scarlet or the various fuchsins > to be satisfactory substitutes for nuclear fast red - they're too dense, > and they don't stain nuclei specifically. > > Nuclear fast red (Kernechtrot, C.I. 60760) is a dye with problems - poor > shelf life both dry and in solution, and it will probably go out of > manufacture eventually. It's used as an aluminum lake for staining nuclei. > > I'm not sure what would serve as an alternative. I'd like to know more > about brazilin (particularly about Anatech's Brazilliant), a catechol dye > similar to hematoxylin, with good specificity for nuclei. > > Bob Richmond > Samurai Pathologist > Maryville TN > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mills at 3scan.com Wed Aug 8 14:52:48 2018 From: mills at 3scan.com (Caroline Miller) Date: Wed, 8 Aug 2018 12:52:48 -0700 Subject: [Histonet] Iron Stain In-Reply-To: References: Message-ID: HI Tasha, The nuclear fast red will stain the nuclei red, the biebrich scarlet is a connective tissue stain and may cover up the (sometimes sparse) prussian blue localization of iron in the tissues. I, personally, would not consider these reagents swappable. In my experience nuclear fast red does last a long time, but I am also not in a lab where expiration dates matter. mills ? On Wed, Aug 8, 2018 at 4:52 AM Campbell, Tasha M. via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Is nuclear fast red the only counter stain for Prussian blue stain? I > have a Masson's trichrome kit and was wondering if the Bierbech Scarlet > could be a counterstain. I won't be doing the iron stain very often at all > so I am trying to keep dry reagents on hand to make up as needed so they do > not expire so quickly. Thanks! > > > > > Tasha Campbell, B.S.,HTL(ASCP) > Frederick Gastroenterology Associates > 310 W. 9th St. > Frederick, MD 21701 > 301-695-6800 ext. 144 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Biology www.3Scan.com 415 2187297 From mills at 3scan.com Wed Aug 8 14:54:34 2018 From: mills at 3scan.com (Caroline Miller) Date: Wed, 8 Aug 2018 12:54:34 -0700 Subject: [Histonet] Iron Stain In-Reply-To: References: Message-ID: Oops, yes, I am mixing up neutral red - and the recipe from Bryan is what I use - it last forever. I have had luck with nuclear fast red from vector - comes made up. That lasts a little longer than lab made. mills ? On Wed, Aug 8, 2018 at 12:52 PM Caroline Miller wrote: > HI Tasha, > > The nuclear fast red will stain the nuclei red, the biebrich scarlet is a > connective tissue stain and may cover up the (sometimes sparse) prussian > blue localization of iron in the tissues. I, personally, would not consider > these reagents swappable. > > In my experience nuclear fast red does last a long time, but I am also not > in a lab where expiration dates matter. > > mills > ? > > On Wed, Aug 8, 2018 at 4:52 AM Campbell, Tasha M. via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Is nuclear fast red the only counter stain for Prussian blue stain? I >> have a Masson's trichrome kit and was wondering if the Bierbech Scarlet >> could be a counterstain. I won't be doing the iron stain very often at all >> so I am trying to keep dry reagents on hand to make up as needed so they do >> not expire so quickly. Thanks! >> >> >> >> >> Tasha Campbell, B.S.,HTL(ASCP) >> Frederick Gastroenterology Associates >> 310 W. 9th St. >> Frederick, MD 21701 >> 301-695-6800 ext. 144 >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > -- > Caroline Miller (mills) > Director of Biology > > www.3Scan.com > 415 2187297 > > > -- Caroline Miller (mills) Director of Biology www.3Scan.com 415 2187297 From Caroline.Pratt at uphs.upenn.edu Wed Aug 8 15:25:56 2018 From: Caroline.Pratt at uphs.upenn.edu (Pratt, Caroline) Date: Wed, 8 Aug 2018 20:25:56 +0000 Subject: [Histonet] Buffy Coat Subspecialty Message-ID: <14D6A469D20B4F4AACC47C3E973C3FA8015ADBCA@UPHMASPHI030.UPHS.PENNHEALTH.PRV> Good afternoon, Does anyone know the correct subspecialty for Buffy Coats on the CLIA certificate? Any feedback would be appreciated! Caroline M. Pratt, MBA Practice Administrator Dermpath 3020 Market Street, Ste 201 Philadelphia, PA 19104 Phone 215-349-8178 Cell 610-800-1381 Fax 215-662-6150 From tony.henwood at health.nsw.gov.au Wed Aug 8 18:29:27 2018 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 8 Aug 2018 23:29:27 +0000 Subject: [Histonet] Iron Stain In-Reply-To: References: Message-ID: <4125cb6319d24437bbd26ff12d106dc8@SVDCMBX-MEX024.nswhealth.net> Try your eosin stain used in your H&E or 1% Neutral red in 2% acetic acid Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Campbell, Tasha M. via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 8 August 2018 9:34 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Iron Stain Is nuclear fast red the only counter stain for Prussian blue stain? I have a Masson's trichrome kit and was wondering if the Bierbech Scarlet could be a counterstain. I won't be doing the iron stain very often at all so I am trying to keep dry reagents on hand to make up as needed so they do not expire so quickly. Thanks! Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From CDavis at che-east.org Fri Aug 10 05:59:00 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Fri, 10 Aug 2018 10:59:00 +0000 Subject: [Histonet] Iron counterstains Message-ID: I enjoy this forum so much, I get to use brain cells I haven't used in years! When I saw your question about other counterstains for Iron Stain I kept thinking quick dip in Eosin (from you H&E) because I thought I remember having to do that in the 90's when we ran our of NFR. Histologist can be so resourceful with the right support. If I remember correctly light Eosin can also be used as a counterstain for GMS, some have used a very pale Hematoxylin but I personally don't care for it because it wasn't enough contrst for me. Tony, thanks for speaking up, it's nice to know I haven't forgot eveything with all the automation. Cassie Davis Message: 6 Date: Wed, 8 Aug 2018 23:29:27 +0000 From: "Tony Henwood (SCHN)" To: "Campbell, Tasha M." Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Iron Stain Message-ID: <4125cb6319d24437bbd26ff12d106dc8 at SVDCMBX-MEX024.nswhealth.net> Content-Type: text/plain; charset="us-ascii" Try your eosin stain used in your H&E or 1% Neutral red in 2% acetic acid Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From CDavis at che-east.org Fri Aug 10 06:51:12 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Fri, 10 Aug 2018 11:51:12 +0000 Subject: [Histonet] ADH 5 Message-ID: <75a06eb487494e5ba5b30959dbd9c13e@che-east.org> Hi Histoland folks, Are/were any of you fine folks using the ADH5 from Biocare on the Ventana instrument? What are you doing now that the have replaced the panel with different antibodies which I already get from Ventana? My doctors really liked the panel, I have a couple of ideas but I wanted to know what other labs are doing so I can offer the doctors more than two options. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From BSolis at Fibrogen.com Fri Aug 10 12:23:40 2018 From: BSolis at Fibrogen.com (Solis, Bryan) Date: Fri, 10 Aug 2018 17:23:40 +0000 Subject: [Histonet] Histonet Digest, Vol 177, Issue 9 In-Reply-To: References: Message-ID: We are looking into purchasing Tissue-tek VIP 6 AI system. Is anyone out there currently using this? Can anyone provide some input/feedback on this? Bryan S. -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Friday, August 10, 2018 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 177, Issue 9 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Iron counterstains (Cassie P. Davis) 2. ADH 5 (Cassie P. Davis) ---------------------------------------------------------------------- Message: 1 Date: Fri, 10 Aug 2018 10:59:00 +0000 From: "Cassie P. Davis" To: histonet Subject: [Histonet] Iron counterstains Message-ID: Content-Type: text/plain; charset="iso-8859-1" I enjoy this forum so much, I get to use brain cells I haven't used in years! When I saw your question about other counterstains for Iron Stain I kept thinking quick dip in Eosin (from you H&E) because I thought I remember having to do that in the 90's when we ran our of NFR. Histologist can be so resourceful with the right support. If I remember correctly light Eosin can also be used as a counterstain for GMS, some have used a very pale Hematoxylin but I personally don't care for it because it wasn't enough contrst for me. Tony, thanks for speaking up, it's nice to know I haven't forgot eveything with all the automation. Cassie Davis Message: 6 Date: Wed, 8 Aug 2018 23:29:27 +0000 From: "Tony Henwood (SCHN)" To: "Campbell, Tasha M." Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Iron Stain Message-ID: <4125cb6319d24437bbd26ff12d106dc8 at SVDCMBX-MEX024.nswhealth.net> Content-Type: text/plain; charset="us-ascii" Try your eosin stain used in your H&E or 1% Neutral red in 2% acetic acid Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 2 Date: Fri, 10 Aug 2018 11:51:12 +0000 From: "Cassie P. Davis" To: histonet Subject: [Histonet] ADH 5 Message-ID: <75a06eb487494e5ba5b30959dbd9c13e at che-east.org> Content-Type: text/plain; charset="iso-8859-1" Hi Histoland folks, Are/were any of you fine folks using the ADH5 from Biocare on the Ventana instrument? What are you doing now that the have replaced the panel with different antibodies which I already get from Ventana? My doctors really liked the panel, I have a couple of ideas but I wanted to know what other labs are doing so I can offer the doctors more than two options. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 177, Issue 9 **************************************** From WWince at STDOM.com Fri Aug 10 16:02:42 2018 From: WWince at STDOM.com (Wanda Wince) Date: Fri, 10 Aug 2018 16:02:42 -0500 Subject: [Histonet] AUTO: Wanda Wince is out of the office (returning 08/13/2018) Message-ID: I am out of the office until 08/13/2018. If this is a pressing matter, please refer to Mara Hanson @6656 or Sheila Sims @ 6758 Note: This is an automated response to your message "Histonet Digest, Vol 177, Issue 9" sent on 08/10/2018 12:00:02 PM. This is the only notification you will receive while this person is away. From cmason100 at yahoo.com Sat Aug 11 20:03:42 2018 From: cmason100 at yahoo.com (Chris Mason) Date: Sun, 12 Aug 2018 01:03:42 +0000 (UTC) Subject: [Histonet] calretinin for Hirschprung's References: <691220990.5512281.1534035822289.ref@mail.yahoo.com> Message-ID: <691220990.5512281.1534035822289@mail.yahoo.com> Would anyone be willing to share their protocol for the sectioning of Hirschprung's cases and the use of calretinin??? Our pathologists want to begin using this technique.? We are thinking of alternating H&E and unstained to be used for calretinin in the case of inconclusive biopsies. Thanks From rsrichmond at gmail.com Sun Aug 12 14:19:36 2018 From: rsrichmond at gmail.com (Bob Richmond) Date: Sun, 12 Aug 2018 15:19:36 -0400 Subject: [Histonet] Calretinin for Hirschsprung's In-Reply-To: References: Message-ID: Chris Mason (where?) asks: >>Would anyone be willing to share their protocol for the sectioning of Hirschprung's cases and the use of calretinin? Our pathologists want to begin using this technique. We are thinking of alternating H&E and unstained to be used for calretinin in the case of inconclusive biopsies.<< Are we talking about frozen sections here? Usually what's wanted is intraoperative consultation about whether neurons are present in the specimen. I never had anything available but H & E. If I'd had any authority, I'd have wanted to do a rapid esterase histochemical stain on the frozen material. Bob Richmond Samurai Pathologist Maryville TN From CDavis at che-east.org Mon Aug 13 10:30:49 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Mon, 13 Aug 2018 15:30:49 +0000 Subject: [Histonet] TMA for MMR panel validation Message-ID: Happy Monday Histonet, I need your help with finding an appropriate TMA to use to validate the MMR panel. The stains are optimized, we just need an good TMA to validate it with. Thank you in advance for any help in this matter! Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From relia1 at earthlink.net Mon Aug 13 11:38:06 2018 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 13 Aug 2018 12:38:06 -0400 Subject: [Histonet] How Was Your Summer Vacation? Message-ID: <09ac01d43324$0379f330$0a6dd990$@earthlink.net> Hi Histonetters! How was your summer vacation? Been anyplace you would like to move to? Whatever you want to do, wherever you want to be, I can help! There is still time this summer to make that move. I have opportunities in all kinds of labs in all areas of the country. Your next position is just a phone call or an e-mail away. All of the opportunities that I represent are permanent full time positions with companies that offer excellent compensation (including generous shift diffs for nights and evenings), and amazing benefits and most offer relocation assistance or a sign on bonus!!!! I have these types of positions open: Histology Sales Support Lead Histology Tech Histology Supervisor Histotechnician Histotechnologist Here are some of the locations that I have opportunities in that I am most excited about: California - Get off the bench! Florida - Yup Florida - (Gulf Coast)! New York -client will help with NY license! New Jersey - All shifts!! ASCP or eligible North Carolina - Days!!! Alabama - Specialty Derm lab Georgia - Days!! Missouri - K.C. - great lab!! Indiana - near Chicago!! ASCP or eligible Histonetters, if you or anyone you know are interested in hearing more about any of these opportunities or have another type of position or area in mind and would like some help in your job search please let me know. Just shoot me an e-mail at relia1 at earthlink.net or give me a call toll free at 866-607-3542 or on my cell at 407-353-5070. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From michael.gudo at morphisto.de Mon Aug 13 12:21:16 2018 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Mon, 13 Aug 2018 19:21:16 +0200 Subject: [Histonet] Specimen cooling during sectioning Message-ID: <7426E18A-8789-4B6D-B858-D5319B492624@morphisto.de> Hello Histonetters, since the summer in Germany is recently quite warm I want to ask about your experiences with specimen cooling during and before sectioning via microtome? Does anyone use Leica CoolClamp or similar cooling units? What do you think about this? Our experience is, that cooling is quite good for sectioning, but the recent tools like CoolClamp or CoolCut do not perform significant cooling, so we tried to construct something more efficient. Now our question is, if this would be of broader interest? Kind regards Michael From tbraud at holyredeemer.com Mon Aug 13 13:03:42 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 13 Aug 2018 18:03:42 +0000 Subject: [Histonet] TMAs for MMR validation Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF6A5C3@HRHEX02-HOS.holyredeemer.local> We used US Biomax TMA CO804A to validate our MMR antibodies. We sent one set to the reference lab where ours were currently being stained, and then stained the other set with the 4 Antibodies. Compared the staining of the 2 and done! I had also called CAP in insure that this method would be acceptable. I hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 2. TMA for MMR panel validation (Cassie P. Davis) Message: 2 Date: Mon, 13 Aug 2018 15:30:49 +0000 From: "Cassie P. Davis" Subject: [Histonet] TMA for MMR panel validation Happy Monday Histonet, I need your help with finding an appropriate TMA to use to validate the MMR panel. The stains are optimized, we just need an good TMA to validate it with. Thank you in advance for any help in this matter! Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org From carl.hobbs at kcl.ac.uk Mon Aug 13 14:11:34 2018 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Mon, 13 Aug 2018 19:11:34 +0000 Subject: [Histonet] Calretinin for Hirschsprung's Message-ID: When I was doing them biopsies ( pre-operative) to establish status, we used a std histochemical method on frozen sections of biopsy material ( visualising acetylcholinesterase activity). It used Osmium to enhance /darken positivity so, NOT recommended these days. I recall somebody leaving the lid off the Osmium ( stored at 4C)?..the fridge plastic lining was BLACK the next day! So, any antibody that identifies nerve fibres and ganglion cells should be equally effective, using IHC/IF? Sure, interpretation is critical ( identifying presence/absence of ganglion cells and "significant" increase in lamina propria of nerve fibres as compensatory mechanism) There must be an internationally recognised/accepted protocol? Which technique does Great Ormond Street Hospital/St Thomas' Hospital use, these days? Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From relia1 at earthlink.net Tue Aug 14 11:04:30 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 14 Aug 2018 12:04:30 -0400 Subject: [Histonet] RELIA Special Job Alert!!! Exciting Opportunities in NC, TN, GA, AL and FL Message-ID: <00fd01d433e8$7beff660$73cfe320$@earthlink.net> Hi Histonetters, How are you? I hope this is the start to a great week for you! I have several exciting opportunities to share and if you aren't interested maybe you know someone who might be! I have been engaged on searches by some of my best clients located in North Carolina, Tennessee, Alabama, Georgia and Florida! My clients offer excellent compensation, benefits and in most cases relocation assistance or sign on bonuses. Here's what they are looking for: . ASCP certified or eligible . Entry level or experienced . Opportunity for Advancement The help I need from you Histonetters is do you know anyone that might be interested in hearing about any of these opportunities? If so could you please forward my e-mail to them or pass their contact information to me? *remember if I place someone you refer to me you will earn a referral bonus! If you are interested in any of these positions please contact me ASAP on my cell/text 407-353-5070 or toll free at 866-607-3542 or via email at relia1 at earthlink.net If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won't release it to anyone without your permission. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From criley at dpspa.com Tue Aug 14 13:26:34 2018 From: criley at dpspa.com (Charles Riley) Date: Tue, 14 Aug 2018 14:26:34 -0400 Subject: [Histonet] Expired SurePath vials Message-ID: Does anyone know how to dispose of expired SurePath vials in Delaware? Or is anyone able to use expired SurePath vials for research testing or any other purpose? Thanks in advance for any replies!! -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From tbraud at holyredeemer.com Tue Aug 14 13:57:58 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 14 Aug 2018 18:57:58 +0000 Subject: [Histonet] detection of ganglion cells and nerve fibers Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF6A844@HRHEX02-HOS.holyredeemer.local> In the past, I've used a rapid acetylcholinesterase on frozen material, but we never used Osmium to enhance staining. I've also used IHC on FFPE sections using s-100 and peripherin. Works great, but it's been awhile, so I don't know if there is something better/easier out there now. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal ------------------------------ Message: 3 Date: Mon, 13 Aug 2018 19:11:34 +0000 From: "Hobbs, Carl" When I was doing them biopsies ( pre-operative) to establish status, we used a std histochemical method on frozen sections of biopsy material ( visualising acetylcholinesterase activity). It used Osmium to enhance /darken positivity so, NOT recommended these days. I recall somebody leaving the lid off the Osmium ( stored at 4C)?..the fridge plastic lining was BLACK the next day! So, any antibody that identifies nerve fibres and ganglion cells should be equally effective, using IHC/IF? Sure, interpretation is critical ( identifying presence/absence of ganglion cells and "significant" increase in lamina propria of nerve fibres as compensatory mechanism) There must be an internationally recognised/accepted protocol? Which technique does Great Ormond Street Hospital/St Thomas' Hospital use, these days? Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From criley at dpspa.com Thu Aug 16 06:59:06 2018 From: criley at dpspa.com (Charles Riley) Date: Thu, 16 Aug 2018 07:59:06 -0400 Subject: [Histonet] Histology slide stainer questions Message-ID: Does anyone out there use the General Data Healthcare? SHUR/Stain? 3030 H&E Slide Stainer? If so can this machine be used to run PAP stains for gyn and non-gyn specimens? I am looking to replace my Gemini stainer and routine H&E stainer with a single unit if possible to free up some lab space. If anyone has any other suggestions it would be greatly appreciated Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From RCazares at schosp.org Thu Aug 16 10:04:23 2018 From: RCazares at schosp.org (Cazares, Ruth) Date: Thu, 16 Aug 2018 10:04:23 -0500 Subject: [Histonet] I have an open part time position Message-ID: Good morning Histonetters! Swedish Covenant Hospital in Chicago has an available 20 hour per pay period position. The hours are 8 pm to midnight, 2 days one week and 3 days the next week. The days are shared with a second histotech and the Fridays are alternated. You would be responsible for cutting biopsies and any immunos, special stains or deepers ordered after the techs have left. If anyone is interested, please apply by following the link below. https://recruiting2.ultipro.com/SWE1003SCHMC/JobBoard/b3bbfb25-ba1f-43ae-a6cb-4f0e2cb70043/?q=hist&o=relevance Any questions, please feel free to contact me directly. Ruth Cazares, HT (ASCP) Histology Supervisor Department of Pathology Swedish Covenant Hospital 5145 N. California Chicago, IL 60625 (773) 878-8200 ext-5190 rcazares at schosp.org From Timothy.Morken at ucsf.edu Thu Aug 16 17:12:38 2018 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 16 Aug 2018 22:12:38 +0000 Subject: [Histonet] FW: Histotechnologist, nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA Message-ID: A position is open for an night-shift histotech at UCSF, San Francisco. Saturdays thru Thursdays. Starting time is flexible between 10:00 pm and 1:00 AM The Histology Lab at UCSF is a full service lab with routine, special stains, IHC and ISH. The workflow is fully barcoded and all equipment is state of the art. Pay ranges (hourly, depending on experience) with night shift differential (to be confirmed by HR on offer): Histotech 1:?? $41.07 to 51.16, $3.45 Histotech 2:? 44.09 to 54.91, $4.50 Histotech 3:? 48.16 to 60.07, $4.50 UCSF also offers extensive benefits:? http://jobs.ucsfmedicalcenter.org/whychoose_employment.html Apply at: http://jobs.ucsfmedicalcenter.org/ Job ID is 16324 Contact Yuri Murphy, Histology Supervisor, ?at ?Yuri.murphy at ucsf.edu with any questions. Job Title Histotechnologist - Pathology-Surgical/Histology (HISTO TCHNO 1, 2 OR 3) Job ID 16324 Job Code 9065, 9066, 9067 Job Family, Technical & Technologist Location: Mount Zion (SF) Weekly Hours: 40 :? 100% Appointment Type: Career Department: Pathology-Surgical/Histology Shift" 8-hour Nights Full/Part Time: Full-Time Union Information: This classification is represented by a union Favorite Job At UCSF Health, our mission of innovative patient care, advanced technology and pioneering research is redefining what's possible for the patients we serve - a promise we share with the professionals who make up our team.? Ranked by U.S. News & World Report as the number one hospital in California - and among the top five in the country - UCSF Health is committed to providing the most rewarding work experience while delivering the best care available anywhere. In an environment that allows for continuous learning and opportunities for professional growth, UCSF Health offers the ideal atmosphere in which to best use your skills and talents. Job Summary Classification of Histotechnologist 1,?2, or 3 will be determined based upon the qualifications of the selected candidate. Under supervision (HT-I, II level) by Senior-level technologists, Lead technologist and the Histology Supervisor, or direction (HT-III) by Lead technologist and the Histology Supervisor, or direction (HT-Lead) by the Histology Supervisor, the incumbent serves as a Histotechnologist in the Histology laboratory. Duties include tissue processing, embedding, paraffin sectioning, H&E staining, Special Staining, specimen receipt and accessioning, Laboratory information system operation, Quality Assurance record keeping, instrument maintenance, intra-operative frozen sections, and other technical duties as assigned, including coverage in the Immunohistochemistry laboratory and Grossing lab as determined by the Lab Manager. Rotates weekly between workstations within the lab. Work schedule is variable to include Saturdays and holiday coverage as scheduled. Incumbent must be able to flex work hours as needed to meet department operational needs and cover work rotations. Required Qualifications . HT 1: o Associate degree?or at least 60 semester hours of academic credit from a regionally accredited college/university, with a combination of 12 semester hours of biology and chemistry, AND one year of experience as a histotechnologist, preferably in a high-volume hospital histology laboratory within the last five years; or completion of an associate degree from an accredited Histotechnology program o Excellent interpersonal and communication skills . HT2: o Current??HT license o Associate degree or at least 60 semester hours of academic credit from a regionally accredited college/university, with a combination of 12 semester hours of biology and chemistry, AND two year of experience as a histotechnologist, in a high-volume hospital histology laboratory within the last five years;?or completion of an associate degree from an accredited Histotechnology program o Demonstrated high volume and high quality sectioning skills o Excellent interpersonal and communication skills . HT3: o Current??HT license o Associate degree or at least 60 semester hours of academic credit from a regionally accredited college/university, with a combination of 12 semester hours of biology and chemistry, AND four years of experience as a histotechnologist in a comparable high-volume hospital histology laboratory within the last five years o Demonstrated high-volume, high-quality sectioning and staining skills o Ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines o Excellent interpersonal and communication . The flexibility to orient and work at all UCSF Medical Center locations Preferred Qualifications HT 1: . One to two years?experience as a Histotechnologist . ASCP certification: HT or HTL licensed HT2: . One to two years experience as a Histotechnologist . ASCP certified HTL HT3: . Five years experience as a Histotechnologist in a large hospital laboratory . ASCP certified HTL Licensure/Certification HT 2 OR 3: . Current?HT license Living Pride Standards Service Excellence . Demonstrates service excellence by following the Everyday PRIDE Guide with the UCSF Medical Center standards and expectations for communication and behavior. These standards and expectations convey specific behavior associated with the Medical Center's values: Professionalism, Respect, Integrity, Diversity and Excellence, and provide guidance on how we communicate with patients, visitors, faculty, staff, and students, virtually everyone, every day and with every encounter. These standards include, but are not limited to: personal appearance, acknowledging and greeting all patients and families, introductions using AIDET, managing up, service recovery, managing delays and expectations, phone standards, electronic communication, team work, cultural sensitivity and competency. ?? . Uses effective communication skills with patients and staff; demonstrates proper telephone techniques and etiquette; acts as an escort to any patient or family member needing directions; shows sensitivity to differences of culture; demonstrates a positive and supportive manner in which patients / families/ colleagues perceive interactions as positive and supportive. Exhibits team work skills to positively acknowledge and recognize other colleagues, and uses personal experiences to model and teach Living PRIDE standards.? . Exhibits tact and professionalism in difficult situations according to PRIDE Values and Practices . Demonstrates an understanding of and adheres to privacy, confidentiality, and security policies and procedures related to Protected Health Information (PHI) or other sensitive and personal information. . Demonstrates an understanding of and adheres to safety and infection control policies and procedures. . Assumes accountability for improving quality metrics associated with department/unit and meeting organizational/departmental targets.? Work Environment . Keeps working areas neat, orderly and clutter-free, including the hallways. Adheres to cleaning processes and puts things back where they belong. Removes and reports broken equipment and furniture. . Picks up and disposes of any litter found throughout entire facility.? . Posts flyers and posters in designated areas only; does not post on walls, doors or windows. . Knows where the Environment of Care Manual is kept in department; corrects or reports unsafe conditions to the appropriate departments. ? . Protects the physical environment and equipment from damage and theft. The flexibility to orient and work at all UCSF Medical Center locations is required. Equal Employment Opportunity The University of California San Francisco is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, protected veteran or disabled status, or genetic information. Further information about the University of California, San Francisco, is available at diversity.ucsf.edu. UCSF seeks candidates whose skills, and personal and professional experience, have prepared them to contribute to our commitment to diversity and excellence, and the communities we serve. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From Timothy.Morken at ucsf.edu Thu Aug 16 17:52:24 2018 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 16 Aug 2018 22:52:24 +0000 Subject: [Histonet] Recall: Histotechnologist, nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA Message-ID: Morken, Timothy would like to recall the message, "Histotechnologist, nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA". From patpxs at gmail.com Thu Aug 16 18:48:40 2018 From: patpxs at gmail.com (P Sicurello) Date: Thu, 16 Aug 2018 16:48:40 -0700 Subject: [Histonet] Unstained slides - how long are they good for? Message-ID: Hello My Fellow Histologists, Happy Friday Eve. The question has come up...... How long are *unstained* slides good for? Not for H&E but tests like IHC and molecular testing. These slides have been cut, stored at room temperature, not sealed in anyway, and kept in a cardboard box. Please let me know what your opinions are and what your retention policy is concerning *unstained* slides. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From jamie at watson-home.com Thu Aug 16 20:12:24 2018 From: jamie at watson-home.com (Jamie Watson) Date: Fri, 17 Aug 2018 01:12:24 +0000 Subject: [Histonet] Unstained slides - how long are they good for? In-Reply-To: References: Message-ID: It depends on the stability of the protein and fixation, some are stable for a week some for years. We use 6 months as a standard. Jamie On August 16, 2018 4:59:34 PM P Sicurello via Histonet wrote: > Hello My Fellow Histologists, > > Happy Friday Eve. > > The question has come up...... How long are *unstained* slides good for? > Not for H&E but tests like IHC and molecular testing. These slides have > been cut, stored at room temperature, not sealed in anyway, and kept in a > cardboard box. > > Please let me know what your opinions are and what your retention policy is > concerning *unstained* slides. > > Thanks oodles. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Thu Aug 16 20:30:20 2018 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Fri, 17 Aug 2018 01:30:20 +0000 Subject: [Histonet] Unstained slides - how long are they good for? In-Reply-To: References: Message-ID: <1c96c1bd524c4ffd9974606bde1aa3d8@SVDCMBX-MEX024.nswhealth.net> Will definitely depend on the antibody you are using. Some references: Jacobs, T. W., Prioleau, J. E., Stillman, I. E., & Schnitt, S. J. (1996). Loss of tumor marker-immunostaining intensity on stored paraffin slides of breast cancer. JNCI: Journal of the National Cancer Institute, 88(15), 1054-1059. Manne, U., MYERS, R. B., SRIVASTAVA, S., & GRIZZLE, W. E. (1997). Re: loss of tumor marker-immunostaining intensity on stored paraffin slides of breast cancer. Journal of the National Cancer Institute, 89(8), 585-586. Bertheau, P., Cazals-Hatem, D., Meignin, V., de Roquancourt, A., V?rola, O., Lesourd, A., ... & Janin, A. (1998). Variability of immunohistochemical reactivity on stored paraffin slides. Journal of clinical pathology, 51(5), 370-374. Olapade-Olaopa, E. O., Mackay, E. H., & Habib, F. K. (1998). Variability of immunohistochemical reactivity on stored paraffin slides. Journal of clinical pathology, 51(12), 943. Wester, K., Wahlund, E., Sundstr?m, C., Ranefall, P., Bengtsson, E., Russell, P. J., ... & Busch, C. (2000). Paraffin section storage and immunohistochemistry: effects of time, temperature, fixation, and retrieval protocol with emphasis on p53 protein and MIB1 antigen. Applied Immunohistochemistry & Molecular Morphology, 8(1), 61-70. van den Broek, L. J., & van de Vijver, M. J. (2000). Assessment of problems in diagnostic and research immunohistochemistry associated with epitope instability in stored paraffin sections. Applied Immunohistochemistry & Molecular Morphology, 8(4), 316-321. Olapade-Olaopa, E. O., Ogunbiyi, J. O., MacKay, E. H., Muronda, C. A., Alonge, T. O., Danso, A. P., ... & Wong, A. J. (2001). Further characterization of storage-related alterations in immunoreactivity of archival tissue sections and its implications for collaborative multicenter immunohistochemical studies. Applied Immunohistochemistry & Molecular Morphology, 9(3), 261-266. Mirlacher, M., Kasper, M., Storz, M., Knecht, Y., D?rm?ller, U., Simon, R., ... & Sauter, G. (2004). Influence of slide aging on results of translational research studies using immunohistochemistry. Modern pathology, 17(11), 1414. DiVito, K. A., Charette, L. A., Rimm, D. L., & Camp, R. L. (2004). Long-term preservation of antigenicity on tissue microarrays. Laboratory investigation, 84(8), 1071. Fergenbaum, J. H., Garcia-Closas, M., Hewitt, S. M., Lissowska, J., Sakoda, L. C., & Sherman, M. E. (2004). Loss of antigenicity in stored sections of breast cancer tissue microarrays. Cancer Epidemiology and Prevention Biomarkers, 13(4), 667-672. Hameed, O., & Humphrey, P. A. (2009). Immunohistochemical evaluation of prostate needle biopsies using saved interval sections vs new recut sections from the block: a prospective comparison. American journal of clinical pathology, 131(5), 683-688. Xie, R., Chung, J. Y., Ylaya, K., Williams, R. L., Guerrero, N., Nakatsuka, N., ... & Hewitt, S. M. (2011). Factors influencing the degradation of archival formalin-fixed paraffin-embedded tissue sections. Journal of Histochemistry & Cytochemistry, 59(4), 356-365. Seidu, M. A., Adams, A. R., Gyasi, R. K., Tettey, Y., Nkansah, D. O., & Wiredu, E. K. (2013). Immunoreactivity of some epitopes in longtime inappropriately stored paraffin-embedded tissues. Journal of Histotechnology, 36(2), 59-64. Nuovo, A. J., Garofalo, M., Mikhail, A., Nicol, A. F., Vianna-Andrade, C., & Nuovo, G. J. (2013). The effect of aging of formalin-fixed paraffin-embedded tissues on the in situ hybridization and immunohistochemistry signals in cervical lesions. Diagnostic Molecular Pathology, 22(3), 164-173. Grillo, F., Bruzzone, M., Pigozzi, S., Prosapio, S., Migliora, P., Fiocca, R., & Mastracci, L. (2017). Immunohistochemistry on old archival paraffin blocks: is there an expiry date?. Journal of Clinical Pathology, jclinpath-2017. Giunchi, F., Degiovanni, A., Daddi, N., Trisolini, R., Dell'Amore, A., Agostinelli, C., ... & Fiorentino, M. (2018). Fading With Time of PD-L1 Immunoreactivity in Non-Small Cells Lung Cancer Tissues: A Methodological Study. Applied Immunohistochemistry & Molecular Morphology, 26(7), 489-494. -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 17 August 2018 9:49 AM To: HistoNet Subject: [Histonet] Unstained slides - how long are they good for? Hello My Fellow Histologists, Happy Friday Eve. The question has come up...... How long are *unstained* slides good for? Not for H&E but tests like IHC and molecular testing. These slides have been cut, stored at room temperature, not sealed in anyway, and kept in a cardboard box. Please let me know what your opinions are and what your retention policy is concerning *unstained* slides. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From Richard.Cartun at hhchealth.org Fri Aug 17 09:19:36 2018 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Fri, 17 Aug 2018 14:19:36 +0000 Subject: [Histonet] Decalcification for Bone Marrow Biopsies Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EA8A6DCA7@HHCEXCHMB03.hhcsystem.org> Help! For years we have used Decal-Stat for decalcifying our bone marrow biopsies with good results. For the past month we have been having problems with tissue loss and morphological damage with these specimens following decalcification. Unfortunately, this was just brought to my attention. Someone told me this morning that the company producing this product was sold and the formulation may have changed. Is that true? Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From Timothy.Morken at ucsf.edu Fri Aug 17 10:16:00 2018 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 17 Aug 2018 15:16:00 +0000 Subject: [Histonet] Unstained slides - how long are they good for? In-Reply-To: References: Message-ID: Paula, since it is variable we strive to not have unstained slides. We had kept them indefinitely, then when storage was overwhelming us we reduced it to 2 months maximum. Now we require request for unstained to be ordered in the system and delivered to the pathologist. We do not hold any in the lab. We recut when new stains are ordered. In the past we had routinely cut extras "just in case" but ended up with thousands of unstained slides that were never used. Instead we trained everyone to reduce wastage and get good sections from a cut block with minimal facing. We have not stored unstained sections for many years and they do not seem to be missed. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, August 16, 2018 4:49 PM To: HistoNet Subject: [Histonet] Unstained slides - how long are they good for? Hello My Fellow Histologists, Happy Friday Eve. The question has come up...... How long are *unstained* slides good for? Not for H&E but tests like IHC and molecular testing. These slides have been cut, stored at room temperature, not sealed in anyway, and kept in a cardboard box. Please let me know what your opinions are and what your retention policy is concerning *unstained* slides. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Fri Aug 17 13:01:40 2018 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 17 Aug 2018 18:01:40 +0000 Subject: [Histonet] Histotech positon open, UCSF Medical Center, nights, Sat to Thursday Message-ID: A position is open for an night-shift histotech at UCSF, San Francisco. Saturdays thru Thursdays. Starting time is flexible between 10:00 pm and 1:00 AM The Histology Lab at UCSF is a full service lab with routine, special stains, IHC and ISH. The workflow is fully barcoded and all equipment is state of the art. Pay ranges (hourly, depending on experience) with night shift differential (to be confirmed by HR on offer): Histotech 1: $41.07 to 51.16, $3.45 Histotech 2: 44.09 to 54.91, $4.50 Histotech 3: 48.16 to 60.07, $4.50 UCSF also offers extensive benefits: http://jobs.ucsfmedicalcenter.org/whychoose_employment.html Apply at: http://jobs.ucsfmedicalcenter.org/ Job ID is 16324 Contact Yuri Murphy, Histology Supervisor, at Yuri.murphy at ucsf.edu with any questions. Job Title Histotechnologist - Pathology-Surgical/Histology (HISTO TCHNO 1, 2 OR 3) Job ID 16324 Job Code 9065, 9066, 9067 Job Family, Technical & Technologist Location: Mount Zion (SF) Weekly Hours: 40 : 100% Appointment Type: Career Department: Pathology-Surgical/Histology Shift" 8-hour Nights Full/Part Time: Full-Time Union Information: This classification is represented by a union Favorite Job At UCSF Health, our mission of innovative patient care, advanced technology and pioneering research is redefining what's possible for the patients we serve - a promise we share with the professionals who make up our team. Ranked by U.S. News & World Report as the number one hospital in California - and among the top five in the country - UCSF Health is committed to providing the most rewarding work experience while delivering the best care available anywhere. In an environment that allows for continuous learning and opportunities for professional growth, UCSF Health offers the ideal atmosphere in which to best use your skills and talents. Job Summary Classification of Histotechnologist 1, 2, or 3 will be determined based upon the qualifications of the selected candidate. Under supervision (HT-I, II level) by Senior-level technologists, Lead technologist and the Histology Supervisor, or direction (HT-III) by Lead technologist and the Histology Supervisor, or direction (HT-Lead) by the Histology Supervisor, the incumbent serves as a Histotechnologist in the Histology laboratory. Duties include tissue processing, embedding, paraffin sectioning, H&E staining, Special Staining, specimen receipt and accessioning, Laboratory information system operation, Quality Assurance record keeping, instrument maintenance, intra-operative frozen sections, and other technical duties as assigned, including coverage in the Immunohistochemistry laboratory and Grossing lab as determined by the Lab Manager. Rotates weekly between workstations within the lab. Work schedule is variable to include Saturdays and holiday coverage as scheduled. Incumbent must be able to flex work hours as needed to meet department operational needs and cover work rotations. Required Qualifications * HT 1: o Associate degree or at least 60 semester hours of academic credit from a regionally accredited college/university, with a combination of 12 semester hours of biology and chemistry, AND one year of experience as a histotechnologist, preferably in a high-volume hospital histology laboratory within the last five years; or completion of an associate degree from an accredited Histotechnology program o Excellent interpersonal and communication skills * HT2: o Current HT license o Associate degree or at least 60 semester hours of academic credit from a regionally accredited college/university, with a combination of 12 semester hours of biology and chemistry, AND two year of experience as a histotechnologist, in a high-volume hospital histology laboratory within the last five years; or completion of an associate degree from an accredited Histotechnology program o Demonstrated high volume and high quality sectioning skills o Excellent interpersonal and communication skills * HT3: o Current HT license o Associate degree or at least 60 semester hours of academic credit from a regionally accredited college/university, with a combination of 12 semester hours of biology and chemistry, AND four years of experience as a histotechnologist in a comparable high-volume hospital histology laboratory within the last five years o Demonstrated high-volume, high-quality sectioning and staining skills o Ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines o Excellent interpersonal and communication * The flexibility to orient and work at all UCSF Medical Center locations Preferred Qualifications HT 1: * One to two years experience as a Histotechnologist * ASCP certification: HT or HTL licensed HT2: * One to two years experience as a Histotechnologist * ASCP certified HTL HT3: * Five years experience as a Histotechnologist in a large hospital laboratory * ASCP certified HTL Licensure/Certification HT 2 OR 3: * Current HT license Living Pride Standards Service Excellence * Demonstrates service excellence by following the Everyday PRIDE Guide with the UCSF Medical Center standards and expectations for communication and behavior. These standards and expectations convey specific behavior associated with the Medical Center's values: Professionalism, Respect, Integrity, Diversity and Excellence, and provide guidance on how we communicate with patients, visitors, faculty, staff, and students, virtually everyone, every day and with every encounter. These standards include, but are not limited to: personal appearance, acknowledging and greeting all patients and families, introductions using AIDET, managing up, service recovery, managing delays and expectations, phone standards, electronic communication, team work, cultural sensitivity and competency. * Uses effective communication skills with patients and staff; demonstrates proper telephone techniques and etiquette; acts as an escort to any patient or family member needing directions; shows sensitivity to differences of culture; demonstrates a positive and supportive manner in which patients / families/ colleagues perceive interactions as positive and supportive. Exhibits team work skills to positively acknowledge and recognize other colleagues, and uses personal experiences to model and teach Living PRIDE standards. * Exhibits tact and professionalism in difficult situations according to PRIDE Values and Practices * Demonstrates an understanding of and adheres to privacy, confidentiality, and security policies and procedures related to Protected Health Information (PHI) or other sensitive and personal information. * Demonstrates an understanding of and adheres to safety and infection control policies and procedures. * Assumes accountability for improving quality metrics associated with department/unit and meeting organizational/departmental targets. Work Environment * Keeps working areas neat, orderly and clutter-free, including the hallways. Adheres to cleaning processes and puts things back where they belong. Removes and reports broken equipment and furniture. * Picks up and disposes of any litter found throughout entire facility. * Posts flyers and posters in designated areas only; does not post on walls, doors or windows. * Knows where the Environment of Care Manual is kept in department; corrects or reports unsafe conditions to the appropriate departments. * Protects the physical environment and equipment from damage and theft. The flexibility to orient and work at all UCSF Medical Center locations is required. Equal Employment Opportunity The University of California San Francisco is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, protected veteran or disabled status, or genetic information. Further information about the University of California, San Francisco, is available at diversity.ucsf.edu. UCSF seeks candidates whose skills, and personal and professional experience, have prepared them to contribute to our commitment to diversity and excellence, and the communities we serve. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From tbraud at holyredeemer.com Fri Aug 17 13:07:10 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 17 Aug 2018 18:07:10 +0000 Subject: [Histonet] Unstained slides Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF6B21E@HRHEX02-HOS.holyredeemer.local> I'm with Tim Morken on this one. The variability of antigenicity in storage is so wide open, and there really is no recent data, so we just make a point of educating our techs on not wasting tissue/levels during sectioning. If the techs feel that the residual tissue in the block is in danger of being exhausted, we communicate with our pathologists on how best to handle any requests. Unstained slides was time, money, and storage and we are better off without them. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 7. Re: Unstained slides - how long are they good for? (Morken, Timothy) Message: 7 Date: Fri, 17 Aug 2018 15:16:00 +0000 From: "Morken, Timothy" To: P Sicurello Subject: Re: [Histonet] Unstained slides - how long are they good for? Paula, since it is variable we strive to not have unstained slides. We had kept them indefinitely, then when storage was overwhelming us we reduced it to 2 months maximum. Now we require request for unstained to be ordered in the system and delivered to the pathologist. We do not hold any in the lab. We recut when new stains are ordered. In the past we had routinely cut extras "just in case" but ended up with thousands of unstained slides that were never used. Instead we trained everyone to reduce wastage and get good sections from a cut block with minimal facing. We have not stored unstained sections for many years and they do not seem to be missed. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, August 16, 2018 4:49 PM To: HistoNet Subject: [Histonet] Unstained slides - how long are they good for? Hello My Fellow Histologists, Happy Friday Eve. The question has come up...... How long are *unstained* slides good for? Not for H&E but tests like IHC and molecular testing. These slides have been cut, stored at room temperature, not sealed in anyway, and kept in a cardboard box. Please let me know what your opinions are and what your retention policy is concerning *unstained* slides. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 177, Issue 16 ***************************************** From john at imebinc.com Fri Aug 17 13:11:17 2018 From: john at imebinc.com (John O'Brien) Date: Fri, 17 Aug 2018 11:11:17 -0700 Subject: [Histonet] Histonet Digest, Vol 177, Issue 16 In-Reply-To: References: Message-ID: <01d001d43655$b1b9c950$152d5bf0$@imebinc.com> Decal Cal Stat company was sold to Stat Lab Medical Products. Last year -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Friday, August 17, 2018 10:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 177, Issue 16 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. FW: Histotechnologist, nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA (Morken, Timothy) 2. Recall: Histotechnologist, nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA (Morken, Timothy) 3. Unstained slides - how long are they good for? (P Sicurello) 4. Re: Unstained slides - how long are they good for? (Jamie Watson) 5. Re: Unstained slides - how long are they good for? (Tony Henwood (SCHN)) 6. Decalcification for Bone Marrow Biopsies (Cartun, Richard) 7. Re: Unstained slides - how long are they good for? (Morken, Timothy) ---------------------------------------------------------------------- Message: 1 Date: Thu, 16 Aug 2018 22:12:38 +0000 From: "Morken, Timothy" To: Histonet Subject: [Histonet] FW: Histotechnologist, nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA Message-ID: Content-Type: text/plain; charset="iso-8859-1" A position is open for an night-shift histotech at UCSF, San Francisco. Saturdays thru Thursdays. Starting time is flexible between 10:00 pm and 1:00 AM The Histology Lab at UCSF is a full service lab with routine, special stains, IHC and ISH. The workflow is fully barcoded and all equipment is state of the art. Pay ranges (hourly, depending on experience) with night shift differential (to be confirmed by HR on offer): Histotech 1:?? $41.07 to 51.16, $3.45 Histotech 2:? 44.09 to 54.91, $4.50 Histotech 3:? 48.16 to 60.07, $4.50 UCSF also offers extensive benefits:? http://jobs.ucsfmedicalcenter.org/whychoose_employment.html Apply at: http://jobs.ucsfmedicalcenter.org/ Job ID is 16324 Contact Yuri Murphy, Histology Supervisor, ?at ?Yuri.murphy at ucsf.edu with any questions. Job Title Histotechnologist - Pathology-Surgical/Histology (HISTO TCHNO 1, 2 OR 3) Job ID 16324 Job Code 9065, 9066, 9067 Job Family, Technical & Technologist Location: Mount Zion (SF) Weekly Hours: 40 :? 100% Appointment Type: Career Department: Pathology-Surgical/Histology Shift" 8-hour Nights Full/Part Time: Full-Time Union Information: This classification is represented by a union Favorite Job At UCSF Health, our mission of innovative patient care, advanced technology and pioneering research is redefining what's possible for the patients we serve - a promise we share with the professionals who make up our team.? Ranked by U.S. News & World Report as the number one hospital in California - and among the top five in the country - UCSF Health is committed to providing the most rewarding work experience while delivering the best care available anywhere. In an environment that allows for continuous learning and opportunities for professional growth, UCSF Health offers the ideal atmosphere in which to best use your skills and talents. Job Summary Classification of Histotechnologist 1,?2, or 3 will be determined based upon the qualifications of the selected candidate. Under supervision (HT-I, II level) by Senior-level technologists, Lead technologist and the Histology Supervisor, or direction (HT-III) by Lead technologist and the Histology Supervisor, or direction (HT-Lead) by the Histology Supervisor, the incumbent serves as a Histotechnologist in the Histology laboratory. Duties include tissue processing, embedding, paraffin sectioning, H&E staining, Special Staining, specimen receipt and accessioning, Laboratory information system operation, Quality Assurance record keeping, instrument maintenance, intra-operative frozen sections, and other technical duties as assigned, including coverage in the Immunohistochemistry laboratory and Grossing lab as determined by the Lab Manager. Rotates weekly between workstations within the lab. Work schedule is variable to include Saturdays and holiday coverage as scheduled. Incumbent must be able to flex work hours as needed to meet department operational needs and cover work rotations. Required Qualifications . HT 1: o Associate degree?or at least 60 semester hours of academic credit from a regionally accredited college/university, with a combination of 12 semester hours of biology and chemistry, AND one year of experience as a histotechnologist, preferably in a high-volume hospital histology laboratory within the last five years; or completion of an associate degree from an accredited Histotechnology program o Excellent interpersonal and communication skills . HT2: o Current??HT license o Associate degree or at least 60 semester hours of academic credit from a regionally accredited college/university, with a combination of 12 semester hours of biology and chemistry, AND two year of experience as a histotechnologist, in a high-volume hospital histology laboratory within the last five years;?or completion of an associate degree from an accredited Histotechnology program o Demonstrated high volume and high quality sectioning skills o Excellent interpersonal and communication skills . HT3: o Current??HT license o Associate degree or at least 60 semester hours of academic credit from a regionally accredited college/university, with a combination of 12 semester hours of biology and chemistry, AND four years of experience as a histotechnologist in a comparable high-volume hospital histology laboratory within the last five years o Demonstrated high-volume, high-quality sectioning and staining skills o Ability to organize and prioritize responsibilities and perform well under pressure to meet deadlines o Excellent interpersonal and communication . The flexibility to orient and work at all UCSF Medical Center locations Preferred Qualifications HT 1: . One to two years?experience as a Histotechnologist . ASCP certification: HT or HTL licensed HT2: . One to two years experience as a Histotechnologist . ASCP certified HTL HT3: . Five years experience as a Histotechnologist in a large hospital laboratory . ASCP certified HTL Licensure/Certification HT 2 OR 3: . Current?HT license Living Pride Standards Service Excellence . Demonstrates service excellence by following the Everyday PRIDE Guide with the UCSF Medical Center standards and expectations for communication and behavior. These standards and expectations convey specific behavior associated with the Medical Center's values: Professionalism, Respect, Integrity, Diversity and Excellence, and provide guidance on how we communicate with patients, visitors, faculty, staff, and students, virtually everyone, every day and with every encounter. These standards include, but are not limited to: personal appearance, acknowledging and greeting all patients and families, introductions using AIDET, managing up, service recovery, managing delays and expectations, phone standards, electronic communication, team work, cultural sensitivity and competency. ?? . Uses effective communication skills with patients and staff; demonstrates proper telephone techniques and etiquette; acts as an escort to any patient or family member needing directions; shows sensitivity to differences of culture; demonstrates a positive and supportive manner in which patients / families/ colleagues perceive interactions as positive and supportive. Exhibits team work skills to positively acknowledge and recognize other colleagues, and uses personal experiences to model and teach Living PRIDE standards.? . Exhibits tact and professionalism in difficult situations according to PRIDE Values and Practices . Demonstrates an understanding of and adheres to privacy, confidentiality, and security policies and procedures related to Protected Health Information (PHI) or other sensitive and personal information. . Demonstrates an understanding of and adheres to safety and infection control policies and procedures. . Assumes accountability for improving quality metrics associated with department/unit and meeting organizational/departmental targets.? Work Environment . Keeps working areas neat, orderly and clutter-free, including the hallways. Adheres to cleaning processes and puts things back where they belong. Removes and reports broken equipment and furniture. . Picks up and disposes of any litter found throughout entire facility.? . Posts flyers and posters in designated areas only; does not post on walls, doors or windows. . Knows where the Environment of Care Manual is kept in department; corrects or reports unsafe conditions to the appropriate departments. ? . Protects the physical environment and equipment from damage and theft. The flexibility to orient and work at all UCSF Medical Center locations is required. Equal Employment Opportunity The University of California San Francisco is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, protected veteran or disabled status, or genetic information. Further information about the University of California, San Francisco, is available at diversity.ucsf.edu. UCSF seeks candidates whose skills, and personal and professional experience, have prepared them to contribute to our commitment to diversity and excellence, and the communities we serve. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center ------------------------------ Message: 2 Date: Thu, 16 Aug 2018 22:52:24 +0000 From: "Morken, Timothy" To: Histonet Subject: [Histonet] Recall: Histotechnologist, nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA Message-ID: Content-Type: text/plain; charset="us-ascii" Morken, Timothy would like to recall the message, "Histotechnologist, nights, Saturday thru Thursday, UCSF Medical Center, San Francisco, CA". ------------------------------ Message: 3 Date: Thu, 16 Aug 2018 16:48:40 -0700 From: P Sicurello To: HistoNet Subject: [Histonet] Unstained slides - how long are they good for? Message-ID: Content-Type: text/plain; charset="UTF-8" Hello My Fellow Histologists, Happy Friday Eve. The question has come up...... How long are *unstained* slides good for? Not for H&E but tests like IHC and molecular testing. These slides have been cut, stored at room temperature, not sealed in anyway, and kept in a cardboard box. Please let me know what your opinions are and what your retention policy is concerning *unstained* slides. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ------------------------------ Message: 4 Date: Fri, 17 Aug 2018 01:12:24 +0000 From: Jamie Watson To: P Sicurello , HistoNet Subject: Re: [Histonet] Unstained slides - how long are they good for? Message-ID: Content-Type: text/plain; charset="us-ascii" It depends on the stability of the protein and fixation, some are stable for a week some for years. We use 6 months as a standard. Jamie On August 16, 2018 4:59:34 PM P Sicurello via Histonet wrote: > Hello My Fellow Histologists, > > Happy Friday Eve. > > The question has come up...... How long are *unstained* slides good for? > Not for H&E but tests like IHC and molecular testing. These slides have > been cut, stored at room temperature, not sealed in anyway, and kept in a > cardboard box. > > Please let me know what your opinions are and what your retention policy is > concerning *unstained* slides. > > Thanks oodles. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Fri, 17 Aug 2018 01:30:20 +0000 From: "Tony Henwood (SCHN)" To: P Sicurello Cc: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Unstained slides - how long are they good for? Message-ID: <1c96c1bd524c4ffd9974606bde1aa3d8 at SVDCMBX-MEX024.nswhealth.net> Content-Type: text/plain; charset="iso-8859-1" Will definitely depend on the antibody you are using. Some references: Jacobs, T. W., Prioleau, J. E., Stillman, I. E., & Schnitt, S. J. (1996). Loss of tumor marker-immunostaining intensity on stored paraffin slides of breast cancer. JNCI: Journal of the National Cancer Institute, 88(15), 1054-1059. Manne, U., MYERS, R. B., SRIVASTAVA, S., & GRIZZLE, W. E. (1997). Re: loss of tumor marker-immunostaining intensity on stored paraffin slides of breast cancer. Journal of the National Cancer Institute, 89(8), 585-586. Bertheau, P., Cazals-Hatem, D., Meignin, V., de Roquancourt, A., V?rola, O., Lesourd, A., ... & Janin, A. (1998). Variability of immunohistochemical reactivity on stored paraffin slides. Journal of clinical pathology, 51(5), 370-374. Olapade-Olaopa, E. O., Mackay, E. H., & Habib, F. K. (1998). Variability of immunohistochemical reactivity on stored paraffin slides. Journal of clinical pathology, 51(12), 943. Wester, K., Wahlund, E., Sundstr?m, C., Ranefall, P., Bengtsson, E., Russell, P. J., ... & Busch, C. (2000). Paraffin section storage and immunohistochemistry: effects of time, temperature, fixation, and retrieval protocol with emphasis on p53 protein and MIB1 antigen. Applied Immunohistochemistry & Molecular Morphology, 8(1), 61-70. van den Broek, L. J., & van de Vijver, M. J. (2000). Assessment of problems in diagnostic and research immunohistochemistry associated with epitope instability in stored paraffin sections. Applied Immunohistochemistry & Molecular Morphology, 8(4), 316-321. Olapade-Olaopa, E. O., Ogunbiyi, J. O., MacKay, E. H., Muronda, C. A., Alonge, T. O., Danso, A. P., ... & Wong, A. J. (2001). Further characterization of storage-related alterations in immunoreactivity of archival tissue sections and its implications for collaborative multicenter immunohistochemical studies. Applied Immunohistochemistry & Molecular Morphology, 9(3), 261-266. Mirlacher, M., Kasper, M., Storz, M., Knecht, Y., D?rm?ller, U., Simon, R., ... & Sauter, G. (2004). Influence of slide aging on results of translational research studies using immunohistochemistry. Modern pathology, 17(11), 1414. DiVito, K. A., Charette, L. A., Rimm, D. L., & Camp, R. L. (2004). Long-term preservation of antigenicity on tissue microarrays. Laboratory investigation, 84(8), 1071. Fergenbaum, J. H., Garcia-Closas, M., Hewitt, S. M., Lissowska, J., Sakoda, L. C., & Sherman, M. E. (2004). Loss of antigenicity in stored sections of breast cancer tissue microarrays. Cancer Epidemiology and Prevention Biomarkers, 13(4), 667-672. Hameed, O., & Humphrey, P. A. (2009). Immunohistochemical evaluation of prostate needle biopsies using saved interval sections vs new recut sections from the block: a prospective comparison. American journal of clinical pathology, 131(5), 683-688. Xie, R., Chung, J. Y., Ylaya, K., Williams, R. L., Guerrero, N., Nakatsuka, N., ... & Hewitt, S. M. (2011). Factors influencing the degradation of archival formalin-fixed paraffin-embedded tissue sections. Journal of Histochemistry & Cytochemistry, 59(4), 356-365. Seidu, M. A., Adams, A. R., Gyasi, R. K., Tettey, Y., Nkansah, D. O., & Wiredu, E. K. (2013). Immunoreactivity of some epitopes in longtime inappropriately stored paraffin-embedded tissues. Journal of Histotechnology, 36(2), 59-64. Nuovo, A. J., Garofalo, M., Mikhail, A., Nicol, A. F., Vianna-Andrade, C., & Nuovo, G. J. (2013). The effect of aging of formalin-fixed paraffin-embedded tissues on the in situ hybridization and immunohistochemistry signals in cervical lesions. Diagnostic Molecular Pathology, 22(3), 164-173. Grillo, F., Bruzzone, M., Pigozzi, S., Prosapio, S., Migliora, P., Fiocca, R., & Mastracci, L. (2017). Immunohistochemistry on old archival paraffin blocks: is there an expiry date?. Journal of Clinical Pathology, jclinpath-2017. Giunchi, F., Degiovanni, A., Daddi, N., Trisolini, R., Dell'Amore, A., Agostinelli, C., ... & Fiorentino, M. (2018). Fading With Time of PD-L1 Immunoreactivity in Non-Small Cells Lung Cancer Tissues: A Methodological Study. Applied Immunohistochemistry & Molecular Morphology, 26(7), 489-494. -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, 17 August 2018 9:49 AM To: HistoNet Subject: [Histonet] Unstained slides - how long are they good for? Hello My Fellow Histologists, Happy Friday Eve. The question has come up...... How long are *unstained* slides good for? Not for H&E but tests like IHC and molecular testing. These slides have been cut, stored at room temperature, not sealed in anyway, and kept in a cardboard box. Please let me know what your opinions are and what your retention policy is concerning *unstained* slides. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. ------------------------------ Message: 6 Date: Fri, 17 Aug 2018 14:19:36 +0000 From: "Cartun, Richard" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Decalcification for Bone Marrow Biopsies Message-ID: <9215BD4B0BA1B44D962A71C758B68D2EA8A6DCA7 at HHCEXCHMB03.hhcsystem.org> Content-Type: text/plain; charset="us-ascii" Help! For years we have used Decal-Stat for decalcifying our bone marrow biopsies with good results. For the past month we have been having problems with tissue loss and morphological damage with these specimens following decalcification. Unfortunately, this was just brought to my attention. Someone told me this morning that the company producing this product was sold and the formulation may have changed. Is that true? Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ------------------------------ Message: 7 Date: Fri, 17 Aug 2018 15:16:00 +0000 From: "Morken, Timothy" To: P Sicurello Cc: Histonet Subject: Re: [Histonet] Unstained slides - how long are they good for? Message-ID: Content-Type: text/plain; charset="us-ascii" Paula, since it is variable we strive to not have unstained slides. We had kept them indefinitely, then when storage was overwhelming us we reduced it to 2 months maximum. Now we require request for unstained to be ordered in the system and delivered to the pathologist. We do not hold any in the lab. We recut when new stains are ordered. In the past we had routinely cut extras "just in case" but ended up with thousands of unstained slides that were never used. Instead we trained everyone to reduce wastage and get good sections from a cut block with minimal facing. We have not stored unstained sections for many years and they do not seem to be missed. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, August 16, 2018 4:49 PM To: HistoNet Subject: [Histonet] Unstained slides - how long are they good for? Hello My Fellow Histologists, Happy Friday Eve. The question has come up...... How long are *unstained* slides good for? Not for H&E but tests like IHC and molecular testing. These slides have been cut, stored at room temperature, not sealed in anyway, and kept in a cardboard box. Please let me know what your opinions are and what your retention policy is concerning *unstained* slides. Thanks oodles. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 177, Issue 16 ***************************************** From john.frazier at roche.com Fri Aug 17 16:34:03 2018 From: john.frazier at roche.com (Frazier, John) Date: Fri, 17 Aug 2018 14:34:03 -0700 Subject: [Histonet] Unstained slides - how long are they good for? Message-ID: I?m a histology workflow consultant that visits many AP laboratories each year. Almost every laboratory has a different retention policy. The average of most laboratories is to hold onto unstained slides for three weeks after final sign out. Typically the unstained slide can be held for a long period of time if used just for morphological staining. However if the unstained slides is going to be used for IHC or molecular testing, the antigenicity of the slide begins degrading at the point of cutting. Typically, however, for a high-quality IHC staining, if stored at room temperature, the unstained slide should not be held much longer than one month. And even at that time frame you will begin to see the degrading of the stain quality. If the slides are kept in a closed box, in refrigerator, they have longer retention. Typically up to 2 to 3 months. I hope this helps Sent from my iPhone > On Aug 16, 2018, at 19:48, P Sicurello wrote: > > Hello My Fellow Histologists, > > Happy Friday Eve. > > The question has come up...... How long are *unstained* slides good for? > Not for H&E but tests like IHC and molecular testing. These slides have > been cut, stored at room temperature, not sealed in anyway, and kept in a > cardboard box. > > Please let me know what your opinions are and what your retention policy is > concerning *unstained* slides. > > Thanks oodles. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > From ckowalcyk at gmail.com Sat Aug 18 11:37:36 2018 From: ckowalcyk at gmail.com (Carol Kowalcyk) Date: Sat, 18 Aug 2018 12:37:36 -0400 Subject: [Histonet] paraffin temperatures Message-ID: When recording paraffin temperatures...do you record all 4 temps. from the processor screen and/ or take the temps by hand in the 4 paraffin baths and record or do you take the temp. of paraffin actually in the retort before draining? Thanks! From rjbuesa at yahoo.com Sat Aug 18 12:31:20 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Sat, 18 Aug 2018 17:31:20 +0000 (UTC) Subject: [Histonet] paraffin temperatures In-Reply-To: References: Message-ID: <1010403856.9555879.1534613480498@mail.yahoo.com> The processor screen temperature reflects the actual reading of an embedded electronic temperature probe and, as such, will be more accurate (always?at the same place, in the same way, with?constant?precision) something rarely obtained manually. Therefore, use the temperature reading from the screen.Ren? On Saturday, August 18, 2018 12:55 PM, Carol Kowalcyk via Histonet wrote: When recording paraffin temperatures...do you record all 4 temps. from the processor screen and/ or take the temps by hand in the 4 paraffin baths and record or do you take the temp. of paraffin actually in the retort before draining? Thanks! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet | | Virus-free. www.avast.com | From tkngflght at yahoo.com Sun Aug 19 09:38:11 2018 From: tkngflght at yahoo.com (Cheryl) Date: Sun, 19 Aug 2018 14:38:11 +0000 (UTC) Subject: [Histonet] Specialty tech position - Dallas area - ASAP References: <205827423.1966590.1534689491502.ref@mail.yahoo.com> Message-ID: <205827423.1966590.1534689491502@mail.yahoo.com> Good morning 'netters! My sister lab is seeking a specialty histotech - more in line with research type work.? ?Day shift, flexible hours and VERY well compensated.? ?Will need to start quickly ( in 2-4 weeks). Frozen section work with a dissection microscope, IHC and some routine and specials staining and a lot of detailed microscope work. Work needs to be PERFECT - not fast.?? If you're a detail-oriented person who gets jazzed by creating gorgeous slides - this is for you. Open to MTs who can cut, as well as HT/HTL applicants.? ?Training for specific techniques and reading protocols provided.? ?Will eventually grow to a team of 4-5 techs in the unit.? The first tech in will help build out, set up and validate -? I'll be your partner for the build and ramp up to production.? It will be fun and rewarding - love that awesome feeling of "I DID THIS!!"?? Send resume - please note that references, background check and drug testing part of hiring process. Cheryl??Cheryl Kerry, HT(ASCP) Full Staff Inc. ? admin at fullstaff.org?281.883.7704 cell?800.756.3309? VM and FAX https://www.facebook.com/TheHistologyCompany/ From john.frazier at roche.com Sun Aug 19 12:37:39 2018 From: john.frazier at roche.com (Frazier, John) Date: Sun, 19 Aug 2018 10:37:39 -0700 Subject: [Histonet] Unstained slides Message-ID: I agree with Tim as well. This is what we advise our clients to do. It takes some coordination with the pathologist, but it is the best strategy for reducing unnecessary unstained slides. In the studies that we have performed only 10% of the unstained slides that are cut are you and the 90% are are it takes some coordination with the pathologist, but it is the best strategy for reducing unnecessary unstained slides. In the studies that we have performed only 10% of the unstained slides that are cut are you and 90% are thrown away thrown away. Several laboratories that I have visited in order to reduce the amount of wasted tissue when refacing the blocks, is to reseal the blocks with liquid paraffin, that have scant or small amounts of tissue in the block, such as the needle core biopsy. Bottom line on this issue is to educate the pathologist, and not water and stain slides except in rare occasions Sent from my iPhone > On Aug 17, 2018, at 14:07, Terri Braud wrote: > > I'm with Tim Morken on this one. The variability of antigenicity in storage is so wide open, and there really is no recent data, so we just make a point of educating our techs on not wasting tissue/levels during sectioning. If the techs feel that the residual tissue in the block is in danger of being exhausted, we communicate with our pathologists on how best to handle any requests. Unstained slides was time, money, and storage and we are better off without them. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Today's Topics: > 7. Re: Unstained slides - how long are they good for? > (Morken, Timothy) > > Message: 7 > Date: Fri, 17 Aug 2018 15:16:00 +0000 > From: "Morken, Timothy" > To: P Sicurello > Subject: Re: [Histonet] Unstained slides - how long are they good for? > > > Paula, since it is variable we strive to not have unstained slides. We had kept them indefinitely, then when storage was overwhelming us we reduced it to 2 months maximum. Now we require request for unstained to be ordered in the system and delivered to the pathologist. We do not hold any in the lab. We recut when new stains are ordered. In the past we had routinely cut extras "just in case" but ended up with thousands of unstained slides that were never used. Instead we trained everyone to reduce wastage and get good sections from a cut block with minimal facing. We have not stored unstained sections for many years and they do not seem to be missed. > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > -----Original Message----- > From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, August 16, 2018 4:49 PM > To: HistoNet > Subject: [Histonet] Unstained slides - how long are they good for? > > Hello My Fellow Histologists, > > Happy Friday Eve. > > The question has come up...... How long are *unstained* slides good for? > Not for H&E but tests like IHC and molecular testing. These slides have > been cut, stored at room temperature, not sealed in anyway, and kept in a > cardboard box. > > Please let me know what your opinions are and what your retention policy is > concerning *unstained* slides. > > Thanks oodles. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 177, Issue 16 > ***************************************** > > > From rjbuesa at yahoo.com Sun Aug 19 13:09:09 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Sun, 19 Aug 2018 18:09:09 +0000 (UTC) Subject: [Histonet] Unstained slides In-Reply-To: References: Message-ID: <1153390626.9774240.1534702149282@mail.yahoo.com> Everything has been pointed out is correct BUT also pivot on "how the unstained slides are kept".Kept in a box their "useful life" is quite short (not beyond 1 week at the most).Kept at -80?C I have used them after years of being stored the principle being of deep-freezing and this is "standard procedure" for IDF "+ controls".Kept in a Coplin jar filled with mineral oil or paraffin covered I have used them after months of being stored the principle being that, isolated from air oxygen, epitopes do not oxidize ("weaken") of if they do, the rate is greatly slowed.On the other hand, usually,?unstained slides are kept for only few days in the event that, lets say within a week, the PT decides to order some special procedure and sometimes it is impossible "return" to the original block many times "almost exhausted".Properly done storing unstained slides are extremely useful.Ren? On Sunday, August 19, 2018 1:52 PM, "Frazier, John via Histonet" wrote: I agree with Tim as well. This is what we advise our clients to do. It takes some coordination with the pathologist, but it is the best strategy for reducing unnecessary unstained slides. In the studies that we have performed only 10% of the unstained slides that are cut are you and the 90% are are it takes some coordination with the pathologist, but it is the best strategy for reducing unnecessary unstained slides. In the studies that we have performed only 10% of the unstained slides that are cut are you and 90% are thrown away thrown away. Several laboratories that I have visited in order to reduce the amount of wasted tissue when refacing the blocks, is to reseal the blocks with liquid paraffin, that have scant or small amounts of tissue in the block, such as the needle core biopsy. Bottom line on this issue is to educate the pathologist, and not water and stain slides except in rare occasions Sent from my iPhone > On Aug 17, 2018, at 14:07, Terri Braud wrote: > > I'm with Tim Morken on this one. The variability of antigenicity in storage is so wide open, and there really is no recent data, so we just make a point of educating our techs on not wasting tissue/levels during sectioning.? If the techs feel that the residual tissue in the block is in danger of being exhausted, we communicate with our pathologists on how best to handle any requests.? Unstained slides was time, money, and storage and we are better off without them. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Today's Topics: >? 7. Re: Unstained slides - how long are they good for? >? ? ? (Morken, Timothy) > > Message: 7 > Date: Fri, 17 Aug 2018 15:16:00 +0000 > From: "Morken, Timothy" > To: P Sicurello > Subject: Re: [Histonet] Unstained slides - how long are they good for? > > > Paula, since it is variable we strive to not have unstained slides. We had kept them indefinitely, then when storage was overwhelming us we reduced it to 2 months maximum. Now we require request for unstained to be ordered in the system and delivered to the pathologist. We do not hold any in the lab. We recut when new stains are ordered. In the past we had routinely cut extras "just in case" but ended up with thousands of unstained slides that were never used. Instead we trained everyone to reduce wastage and get good sections from a cut block with minimal facing. We have not stored unstained sections for many years and they do not seem to be missed. > > Tim Morken > Pathology Site Manager, Parnassus > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology > UC San Francisco Medical Center > > > -----Original Message----- > From: P Sicurello via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Sent: Thursday, August 16, 2018 4:49 PM > To: HistoNet > Subject: [Histonet] Unstained slides - how long are they good for? > > Hello My Fellow Histologists, > > Happy Friday Eve. > > The question has come up......? How long are *unstained* slides good for? > Not for H&E but tests like IHC and molecular testing.? These slides have > been cut, stored at room temperature, not sealed in anyway, and kept in a > cardboard box. > > Please let me know what your opinions are and what your retention policy is > concerning *unstained* slides. > > Thanks oodles. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material.? Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited.? If you received this e-mail in error, > please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 177, Issue 16 > ***************************************** > > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet | | Virus-free. www.avast.com | From carl.hobbs at kcl.ac.uk Sun Aug 19 13:40:41 2018 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sun, 19 Aug 2018 18:40:41 +0000 Subject: [Histonet] Unstained slides - how long are they good for? Message-ID: I agree with Jamie Only a few Ags are "oxidised" ( that's the term used, I recall) but, don't let it be YOUR protein of interest. If you really are concerned, cut fresh sections and immunostain along with your stored sections. Imho: cut as few sections as you need. Store any unused at 4C Best wishes Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From jamie at watson-home.com Sun Aug 19 14:15:55 2018 From: jamie at watson-home.com (Jamie Watson) Date: Sun, 19 Aug 2018 19:15:55 +0000 Subject: [Histonet] Unstained slides - how long are they good for? In-Reply-To: References: Message-ID: To expand on what we do at our research lab; we use 6 months as a standard maximum age of unstained slides. Also in the staining protocol for each antibodyl we have a specific shelf life for the diluted Antibody and a maximum age of unstained slides. Jamie From marktarango at gmail.com Sun Aug 19 17:02:50 2018 From: marktarango at gmail.com (Mark Tarango) Date: Sun, 19 Aug 2018 15:02:50 -0700 Subject: [Histonet] Unstained slides - how long are they good for? In-Reply-To: References: Message-ID: Hi Everyone! I have seen unstained slides save a patient from re-biopsy many times. Usually it will be a case where a patient has a known diagnosis, like lung cancer. In these types of cases after diagnosis molecular testing (and IHC for PD-L1) is usually ordered. There have been countless times that I can recall where a few unstained slides on a biopsy with scant tumor was able to get us results for PD-L1, ALK FISH, and ROS1 FISH. Often in these types of a cases a touch prep can be used for Next Generation Sequencing or PCR testing like EGFR or BRAF, allowing for the full panel of molecular tests to be performed. For cases that are small specimens I would prefer to have unstained slides to fall back on for patient convenience, client satisfaction, and quicker TAT of molecular testing. Re-biopsy and re-diagnosing the new sample costs money to the patient and payers and having some unstained slides can often save those costs providing more value to the original biopsy. Sometimes when we try to save money in the lab it can result in more money being spent on healthcare overall. It is true that some antigens become more difficult to stain over time and storage is an important consideration. Limiting the production of unstained slides to small and scant needle may make storage more practical. Just some more things to consider. Sincerely, Mark Tarango On Thu, Aug 16, 2018 at 4:48 PM, P Sicurello via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello My Fellow Histologists, > > Happy Friday Eve. > > The question has come up...... How long are *unstained* slides good for? > Not for H&E but tests like IHC and molecular testing. These slides have > been cut, stored at room temperature, not sealed in anyway, and kept in a > cardboard box. > > Please let me know what your opinions are and what your retention policy is > concerning *unstained* slides. > > Thanks oodles. > > Sincerely, > > Paula Sicurello, HTL (ASCP)CM > > Histotechnology Specialist > > UC San Diego Health > > 200 Arbor Drive > > San Diego, CA 92103 > > (P): 619-543-2872 > > > > *Confidentiality Notice*: The information transmitted in this e-mail is > intended only for the person or entity to which it is addressed and may > contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this e-mail in error, > please contact the sender and delete the material from any computer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tkngflght at yahoo.com Mon Aug 20 19:29:32 2018 From: tkngflght at yahoo.com (Cheryl) Date: Tue, 21 Aug 2018 00:29:32 +0000 (UTC) Subject: [Histonet] Seeking a Microm HM450 - vendors welcome to respond References: <1957387711.72537.1534811372842.ref@mail.yahoo.com> Message-ID: <1957387711.72537.1534811372842@mail.yahoo.com> Histonetters - seeking to purchase two Microm HM 450 sliding microtomes.?? Need a vendor contact, please? ?Cheryl Kerry, HT(ASCP)? Operations, ADG Houston?281.661.1825 x 102 From relia1 at earthlink.net Tue Aug 21 12:27:43 2018 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 21 Aug 2018 13:27:43 -0400 Subject: [Histonet] Team Building - what qualities make someone a good fit in YOUR lab? Message-ID: <000801d43974$45fbdab0$d1f39010$@earthlink.net> Dear Histonetters, I hope you are having a great week! I recently told you about an article I wrote entitled "Would you want YOU for a coworker. It appeared in the NSH Career Newsletter. I was thinking about some of the responses that I got and came up with another question for you: What qualities would YOU like to see in a new coworker? Histonetters, If you were asked this question how would you answer it? The ball is in your court! If your lab has an opening I can help and I can recruit for the BEST fit for your lab. Do you need someone strong in grossing? IHC? A strong cutter or embedder? A night person, a day person? Someone who is responsible, respectful, professional, flexible, a great communicator, someone who is easy to get along with? Your colleagues the people that I help find new opportunities want to be in labs where they are comfortable and part of my job is to find them the perfect place to work. Let's do this together and everybody wins!! Let me help you. If your lab is looking for a histotech today, tomorrow, in 3 months, 6 months, a year, 5 years. I am here for you. My best day at work is every day since my passion is to help put the best teams together! All you have to do is one of two things: 1. Put me in touch with your hiring manager OR 2. Send me their name, phone number and email address and I will contact them (referred by you or confidentially your choice). Even if your lab isn't hiring - Don't forget to shoot me an email back with the answer to that question! -I think it will be a great conversation starter at NSH!! Just in case you or someone you know is interested in looking into a new opportunity . Here is a list of where my best opportunities are located: California Tennessee - IHC! Alabama Missouri North Carolina New York New Jersey Indiana - near Chicago!! All of these opportunities are full time permanent positions with some of the leading employers nationwide. There are 1st, 2nd and 3rd shift positions open. Experienced and entry level supervisory and tech and ASCP or eligible!! My clients offer excellent compensation, benefits and relocation/sign on bonuses. And they can't wait to speak to you!!! Please contact me at relia1 at earthlink.net or toll free at 866-607-3542 or call/text me on my cell at 407-353-5070 if you are interested in more information on any of these positions or if you would like for me to work on a custom job search for you. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From boznpl at aol.com Tue Aug 21 21:33:51 2018 From: boznpl at aol.com (Laurie Colbert) Date: Tue, 21 Aug 2018 22:33:51 -0400 Subject: [Histonet] Special stains and hoods Message-ID: <1655f7b270c-1eb9-3690@webjasstg-vaa45.srv.aolmail.net> I would like to know how many people perform (manual) routine special stains under a hood.? We do basic specials - Giemsa, AB/PAS, GMS, Trichrome, Muci - but we do them on the counter, not under a hood.? We have been told the staining needs to be done under a hood, but I have never done that in my entire career. From DKnutson at primecare.org Wed Aug 22 09:52:53 2018 From: DKnutson at primecare.org (Knutson, Deanne) Date: Wed, 22 Aug 2018 09:52:53 -0500 Subject: [Histonet] MSH2 and MSH6 Loss of Expression Message-ID: <1E0E2B14C709174B8AC2BE0AE7F768330150C4D49D60@EXCHANGE2K7.staprimecare.org> Fellow Histonet Members - We are in the process of moving our IHC bench from the Leica BOND to the ROCHE ULTRA and are in the mist of antibody validations. We are having great difficulty in finding negatives to validate for MSH2 and MSH6. Would anyone have any loss of expression MSH2 and MSH6 appropriate validation specimens or cut slides they are willing to share? Deanne Knutson Supervisor Anatomic Pathology 900 East Broadway Avenue, Bismarck, ND 58501-4520 P 701-530-6730 I F 701-530-6735 dknutson at primecare.org st.alexius.org [X] "Let All Be Received as Christ." ________________________________ This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. From Shannon.Logan at bellin.org Wed Aug 22 10:12:58 2018 From: Shannon.Logan at bellin.org (Logan, Shannon) Date: Wed, 22 Aug 2018 15:12:58 +0000 Subject: [Histonet] Special stains and hoods In-Reply-To: <1655f7b270c-1eb9-3690@webjasstg-vaa45.srv.aolmail.net> References: <1655f7b270c-1eb9-3690@webjasstg-vaa45.srv.aolmail.net> Message-ID: <5082fad128184528a06eebf65fe46aae@BAPWEXCH001b.bellin.com> That is certainly an odd request? I have never performed any special stains under a hood any place I have ever worked. Always done on a counter top near a sink? Is someone in your department extremely sensitive to odors? Or have asthma? We have a tech who experiences attacks if there are certain chemicals in the air however, she has never complained about the special stains odors. Shannon H. Logan B.S., HTL (ASCP) Pathology Department Bellin Health Memorial Hospital 744 South Webster Avenue Green Bay, WI 54305-3400 920-433-3653 X3727 From: Laurie Colbert via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 21, 2018 9:34 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Special stains and hoods CAUTION: This email originated from outside of Bellin. Do not click on any links or open any attachments unless you recognize the sender and know the contents are safe. Questions? Contact the Service Desk @ x258600 or (920) 436-8600 I would like to know how many people perform (manual) routine special stains under a hood. We do basic specials - Giemsa, AB/PAS, GMS, Trichrome, Muci - but we do them on the counter, not under a hood. We have been told the staining needs to be done under a hood, but I have never done that in my entire career. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Timothy.Morken at ucsf.edu Wed Aug 22 10:47:09 2018 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Wed, 22 Aug 2018 15:47:09 +0000 Subject: [Histonet] Special stains and hoods In-Reply-To: <1655f7b270c-1eb9-3690@webjasstg-vaa45.srv.aolmail.net> References: <1655f7b270c-1eb9-3690@webjasstg-vaa45.srv.aolmail.net> Message-ID: I guess the question is, who told you this and what is their reference? Most special stains don't require a hood. Only those that have volatile chemicals or odors that may be offensive (ie, ammonium sulfide). We have some areas we do specials without a hood. Our main special stain areas have a "comfort" hood that is not a real chemical hood but more like a hood in an oven hood in a commercial kitchen. They are not certified or checked in any way and we do not claim they are safety hoods. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Laurie Colbert via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, August 21, 2018 7:34 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Special stains and hoods I would like to know how many people perform (manual) routine special stains under a hood.? We do basic specials - Giemsa, AB/PAS, GMS, Trichrome, Muci - but we do them on the counter, not under a hood.? We have been told the staining needs to be done under a hood, but I have never done that in my entire career. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CDavis at che-east.org Wed Aug 22 12:42:58 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Wed, 22 Aug 2018 17:42:58 +0000 Subject: [Histonet] Special stains and hoods Message-ID: <80964dfe6c044e3a9e6d12b047fe9fb6@che-east.org> 25+ years in Histology, half the places I worked had vents on the wall near the countertop where we did specials, otherwise it was on the counterclosest to the sink. The only times we were time doing anything under a hood was for frozen section staining because of the fresh tissue, mixing sulfer chemicals for muscle enzyme studies, diluting acids and the bouins when we microwaved it. As long as there is a sink under the hood too, I wouldn't mind, though. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From tkngflght at yahoo.com Wed Aug 22 12:59:15 2018 From: tkngflght at yahoo.com (Cheryl) Date: Wed, 22 Aug 2018 17:59:15 +0000 (UTC) Subject: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? References: <1733736332.1243496.1534960755814.ref@mail.yahoo.com> Message-ID: <1733736332.1243496.1534960755814@mail.yahoo.com> Help?? ?we have a student with one of the online schools and we're doing specials like it is 1972.? She has to do a Al Blue 2.5 w Hyaluronidase digestion.? ?We haven't done this since the development of clinical IHC two dozen years ago - Is there any other way to acheive this outcome on a colon slide or do I have to order the reagent? Even your best guess is welcome as we didn't get ahead enough to order the reagent and she'll end up submitting a null response (and it will be largely my fault :(? )?Cheryl Kerry, HT(ASCP)? ADG Houston Path From tbraud at holyredeemer.com Wed Aug 22 13:21:14 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 22 Aug 2018 18:21:14 +0000 Subject: [Histonet] special Stains under a hood Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF6BB0C@HRHEX02-HOS.holyredeemer.local> I have never (3 different places, 30+ years) heard of requiring special stains to be performed under a hood. Certainly, there are a few reagents that might need benefit from a hood (concentrated Ammonium Hydroxide) but generally, they are performed on an open counter next to a sink. If you are getting pushback, check to see if Micro is doing their gram stain under a hood? Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal From PREISZNE at mail.etsu.edu Wed Aug 22 13:59:44 2018 From: PREISZNE at mail.etsu.edu (Preiszner, Johanna) Date: Wed, 22 Aug 2018 18:59:44 +0000 Subject: [Histonet] cadaver tissue processing for histology Message-ID: Hi, I got some cadaver tissue floating in "LTP embalming fluid w/ phenol". Can I put those straight into the processor? Or has to be rinsed/soaked before? If so what should I use? My Internet searches did not bring up anything useful. Thank you! Hanna Preiszner ETSU/QCOM Dept of Pathology From Jessica.Vacca at HCAhealthcare.com Thu Aug 23 12:09:19 2018 From: Jessica.Vacca at HCAhealthcare.com (Vacca Jessica) Date: Thu, 23 Aug 2018 17:09:19 +0000 Subject: [Histonet] Windsurg Reports Message-ID: Afternoon, Looking to see if anyone has some tipsheets on how to pull embedding logs or accession logs from winsurg. I am by no means familiar with the system so if you could send me step by step instructions I would really appreciate it!! Thanks! Jessica Vacca HCA Epic Anatomic Pathology Lead From tbraud at holyredeemer.com Thu Aug 23 13:01:59 2018 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 23 Aug 2018 18:01:59 +0000 Subject: [Histonet] LTP fluid with Phenol Message-ID: <48E053DDF6CE074DB6A7414BA05403F8ECF6BDFE@HRHEX02-HOS.holyredeemer.local> Here is the approximate chemical make-up of LTP with Phenol. (LTP stands for Long Term Preservation) Ethanol 48.% Formaldehyde 20.% Ethylene Glycol 4.% Phenol 11.% Glutaraldehyde 4.% Due to the presence of Ethylene Glycol, I would let the tissue set in a separate container of 10% Neutral Buffered Formalin for 24 hours before processing as usual. I hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal 4. cadaver tissue processing for histology (Preiszner, Johanna) Message: 4 Date: Wed, 22 Aug 2018 18:59:44 +0000 From: "Preiszner, Johanna" Subject: [Histonet] cadaver tissue processing for histology Hi, I got some cadaver tissue floating in "LTP embalming fluid w/ phenol". Can I put those straight into the processor? Or has to be rinsed/soaked before? If so what should I use? My Internet searches did not bring up anything useful. Thank you! Hanna Preiszner ETSU/QCOM Dept of Pathology From carl.hobbs at kcl.ac.uk Thu Aug 23 13:32:13 2018 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Thu, 23 Aug 2018 18:32:13 +0000 Subject: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? Message-ID: Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab pH1 ( highly sulphated mucins only) on a sequential slide? Sure, instead use Alcian blue CEC method? I will be interested to read other responses to your request. Mucin-illy carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From carl.hobbs at kcl.ac.uk Thu Aug 23 13:41:17 2018 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Thu, 23 Aug 2018 18:41:17 +0000 Subject: [Histonet] cadaver tissue processing for histology Message-ID: Rinse in dist water ( or tap water) x3 over 30 mins No big deal to do this but nice to get rid of extra smells in your processing reagents ( depending on where you start processing: I start in 90% alcohol....sure, I ask for recd specimens to be rinsed in water then transferred to 70% alcohol? before I will process them) Hopefully, you don't want to do IHC as inclusion of Glut. is counterproductive. Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From jmacdonald at mtsac.edu Thu Aug 23 13:52:52 2018 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Thu, 23 Aug 2018 18:52:52 +0000 Subject: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? In-Reply-To: References: Message-ID: The hyaluronidase is digesting out hyaluronic acid, a component found in connective tissue mucins. This reaction is used to differentiate connective tissue mucins from epithelial mucins. Hyaluronic acid and epithelial sialomucins will give the same staining results, both positive for alcian blue pH 2.5 and negative for pH 1.0. -----Original Message----- From: Hobbs, Carl via Histonet Sent: Thursday, August 23, 2018 11:32 AM To: histonet Subject: Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab pH1 ( highly sulphated mucins only) on a sequential slide? Sure, instead use Alcian blue CEC method? I will be interested to read other responses to your request. Mucin-illy carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Thu Aug 23 14:11:01 2018 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Thu, 23 Aug 2018 19:11:01 +0000 Subject: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? In-Reply-To: References: , Message-ID: You are right! Thank you for correcting me. Surely sialomucins will be positive for PAS and H.acid will be negative? Curious-illy Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ________________________________ From: Mac Donald, Jennifer Sent: 23 August 2018 19:52:52 To: Hobbs, Carl Cc: histonet at lists.utsouthwestern.edu Subject: RE: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? The hyaluronidase is digesting out hyaluronic acid, a component found in connective tissue mucins. This reaction is used to differentiate connective tissue mucins from epithelial mucins. Hyaluronic acid and epithelial sialomucins will give the same staining results, both positive for alcian blue pH 2.5 and negative for pH 1.0. -----Original Message----- From: Hobbs, Carl via Histonet Sent: Thursday, August 23, 2018 11:32 AM To: histonet Subject: Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab pH1 ( highly sulphated mucins only) on a sequential slide? Sure, instead use Alcian blue CEC method? I will be interested to read other responses to your request. Mucin-illy carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://emea01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=01%7C01%7Ccarl.hobbs%40kcl.ac.uk%7Cc3bfd2048e1b4508a26b08d60929a33c%7C8370cf1416f34c16b83c724071654356%7C0&sdata=CTVwv5AklHrbGKIoml5W4NVoYJ7Skdfro9F%2FXE0uuWM%3D&reserved=0 From erin.mccarthy at tempus.com Thu Aug 23 16:21:20 2018 From: erin.mccarthy at tempus.com (Erin McCarthy) Date: Thu, 23 Aug 2018 16:21:20 -0500 Subject: [Histonet] LDT question Message-ID: Hi All, I have a validation question - we are looking into buying an RUO rated IHC stainer for a future multiplexing project. Roche's Discovery Ultra to be specific. I have a pathologist that is convinced that we can run our clinical antibodies on it as an LDT and still bill for the work. The material I have read up to this point regarding that instrument explicitly states that no slides can be run for diagnostic purposes. The instrument has software that allows you to use it to mimic the IVD instruments' software capabilities so you can validate a protocol on it, then move it right over to the Benchmark so I can see why the idea that it can run our IVD items seems plausable. However what I need to know is which is correct? I do not want to do something that I am uncomfortable doing without checking with others in my field first. Thanks! -- Erin McCarthy, HT (ASCP) Histotechnician Tempus Labs 600 W. Chicago Ave. Chicago IL 60654 Ph:(312) 638-6344 Ext.3835 -- This email and any attachments may contain privileged and confidential information and/or protected health information (PHI) that is protected by federal and state privacy laws.? It is intended solely for the use of Tempus Labs and the recipient(s) named above.? Nothing contained in this communication and any attachments thereto is intended to waive any privileges or rights of confidentiality.? If you are not the recipient, or the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any review, dissemination, distribution, printing or copying of this email message and/or any attachments is strictly prohibited.?* If you have received this transmission in error, please notify us immediately at?**(855)-442-8305**? and permanently delete this email and any attachments*. From CDavis at che-east.org Fri Aug 24 09:02:28 2018 From: CDavis at che-east.org (Cassie P. Davis) Date: Fri, 24 Aug 2018 14:02:28 +0000 Subject: [Histonet] trying to track down a technique Message-ID: <6e793c1cb7ac495492ba7900058a5f70@che-east.org> Good morning Histonet Histology Folks, A few years ago a vendor was doing an onsite workflow analysis and showed us a video of a site that saved their unstained sections on paper rather than glass slides and if they needed the tissue later they were able to float the section off the paper and remount it onto a glass slide. Does anyone have any information on this technique? I am especially curious as to what kind of paper and paraffin are used and how long is the paper with the section viable? thank you in advance for any information you can provide, Cassie Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From relia1 at earthlink.net Fri Aug 24 09:55:02 2018 From: relia1 at earthlink.net (Pam Barker) Date: Fri, 24 Aug 2018 10:55:02 -0400 Subject: [Histonet] Histology Lab Manager - State of the Art Lab in Colorado - A RELIA exclusive! Message-ID: <006a01d43bba$700e05b0$502a1110$@earthlink.net> Hi Histonetters, How are you? I hope you are having a nice day. I am reaching out to you for help because I am presently on a search for a great lab that is in need of a Histology Lab Manager in Denver, CO!! This is a RELIA Exclusive!! My client offers a state of the art lab, excellent compensation(including salary and incentives), great benefits, relocation assistance and a great group of people to work with. The help I need is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them? Histonetter, Would you possibly be interested in the opportunity? If you or anyone you know might be interested in hearing more about this opportunity Please call me at 866-607-3542 or e-mail me at relia1 at earthlink.net Remember if you refer someone I place You will receive a referral reward bonus. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From carl.hobbs at kcl.ac.uk Fri Aug 24 13:21:29 2018 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Fri, 24 Aug 2018 18:21:29 +0000 Subject: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? In-Reply-To: <12A3CD99-C981-48AB-A1F6-24C165F234B9@morphisto.de> References: , <12A3CD99-C981-48AB-A1F6-24C165F234B9@morphisto.de> Message-ID: Vielen dank, Michael Very kind of you to send this to me. Alcian blue is indeed an interesting dye I thought that it was no longer manufactured? I am wrong, obviously. I have used the CEC method many years ago when analysing articular cartilages and it is good to see that it is still a valid method to differentiate the various acidic mucins Alles Gute carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ________________________________ From: Dr. Michael Gudo (Morphisto GmbH) Sent: 24 August 2018 11:22:55 To: Hobbs, Carl Cc: histonet Subject: Re: [Histonet] Alcian Blue digested w Hyaluronidase - any alternatives? Hello Carl, Alcianblue is q quite interesting stain! Maybe this selection of a presentation helps you to find a suitable method. I attach you a PDF-document to this email. The slides are in german, but I think you will understand them anyway. Kind regards Michael Am 23.08.2018 um 20:32 schrieb Hobbs, Carl via Histonet >: Perhaps Alcian blue at pH3 ( all acidic mucins) on one slide/section and Ab pH1 ( highly sulphated mucins only) on a sequential slide? Sure, instead use Alcian blue CEC method? I will be interested to read other responses to your request. Mucin-illy carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. 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From john.garratt at ciqc.ca Fri Aug 24 19:23:04 2018 From: john.garratt at ciqc.ca (john.garratt at ciqc.ca) Date: Fri, 24 Aug 2018 17:23:04 -0700 Subject: [Histonet] Canadian Labs - Let us know the challenges you face introducing PD-L1 testing Message-ID: <005201d43c09$ca3bb5b0$5eb32110$@ciqc.ca> We would like to request participation of ALL Canadian laboratories in this survey. Even if you are not currently testing for PD-L1, please continue to read. This survey is designed by the Canadian Immunohistochemistry Quality Control scheme (cIQc) to understand: i. The challenges that laboratories face in introducing PD-L1 testing, ii. The overall landscape in predictive biomarker development in clinical IHC laboratories. Click on https://www.surveymonkey.com/r/P2XTCN8 to start the survey. The information we receive from this survey will help cIQc develop a fit-for-purpose proficiency testing program and improve our ability to assist laboratories. The target participants for this survey are pathologists, laboratory scientists/technologists, researchers, and also oncologists if they are actively involved in predictive biomarker development. Therefore, please feel free to forward the survey link below to your oncologist colleagues, especially if they work in close collaboration with you on new test development. We would like to emphasize that this survey is open both to those who currently test for PD-L1 and those who do not. Feedback from both groups is important to us. Your participation in this survey is completely anonymous and none of the responses will be connected to identifying information and the results will be disseminated in aggregate form through cIQc. If the results are such that we believe they may be of interest to the national/international community, then we will also attempt to submit for publication in a peer-reviewed journal. The survey will take 8 - 10 minutes to complete. Click on https://www.surveymonkey.com/r/P2XTCN8 to start the survey. The survey closes September 15th 2018 If you have any questions about this survey, or difficulty in accessing the site or completing the survey, please contact john.garratt at ciqc.ca Thank you in advance for your time and consideration. From brannon at alliedsearchpartners.com Mon Aug 27 10:11:27 2018 From: brannon at alliedsearchpartners.com (Brannon Owens) Date: Mon, 27 Aug 2018 15:11:27 +0000 Subject: [Histonet] Histology job openings Nationwide Message-ID: Good morning! We are currently working to fill several histology openings Nationwide. 1. Histotechnician/Histotechnologist - Atlanta, GA early morning day shift 2. Histotechnician/Histotechnologist - central Pennsylvania 1st shift OR 2nd shift Additional openings listed on our website - check us out and see if we have an opening to fit you!! Thank you, 2018 Forbes 100 Best Professional Recruitment Firms in American and the only Laboratory Staffing Specific Firm on List! https://www.forbes.com/best-professional-recruiting-firms/list/ Brannon Owens VP/Director of Recruitment Allied Search Partners LinkedIn: https://www.linkedin.com/in/jbrannonowens/ http://www.alliedsearchpartners.com T: 888.388.7571 ext. 106 Direct Line: 407.413.9421 F: 888.388.7572 From boznpl at aol.com Mon Aug 27 10:30:17 2018 From: boznpl at aol.com (Laurie Colbert) Date: Mon, 27 Aug 2018 11:30:17 -0400 Subject: [Histonet] Per diem Histotech position at Path MD Message-ID: <1657c01cccb-1ebc-c50@webjasstg-vab21.srv.aolmail.net> Path MD is looking for a per diem histotech to work Saturdays and as needed during the week.? Must be ASCP registered and be able to start between midnight and 2:00 am and also be able to work during the holidays. Path MD is a state of the art Pathology Lab located in West Hollywood.? We are looking for a team player that is reliable and responsible.? Salary based on experience.? Candidates may send their resume to Laurie Redmond at?both of the following emails:? boznpl at aol.com? ? ?and? ? lredmond at pathmdlabs.com From relia1 at earthlink.net Mon Aug 27 10:52:18 2018 From: relia1 at earthlink.net (Pam Barker) Date: Mon, 27 Aug 2018 11:52:18 -0400 Subject: [Histonet] Histo Tech Days Full time in PA !! Can you help? Message-ID: <001c01d43e1d$ef5a4030$ce0ec090$@earthlink.net> Hi Histonetters, How are you? I hope you are looking forward to an amazing Labor Day Weekend!! I have an exciting opportunity to share and if you aren?t interested maybe you know someone who might be! I have been engaged on an exclusive search by one of my best clients located in Pottstown, PA that is in need of a: Full Time Day Shift Histotech!! This is a full time permanent position in a hospital environment. My client is looking for a strong histotech that is ASCP certified HT/HTL, with strong routine histology. CLIA qualified to gross and grossing experience is a plus. They are offering an excellent compensation package. The help I need from you Histonetters is do you know anyone that might be interested in hearing about this opportunity? If so could you please forward my e-mail to them or pass their contact information to me? *remember if I place someone you refer to me you will earn a referral bonus! If you are interested in this position please contact me ASAP on my cell/text 407-353-5070 or toll free at 866-607-3542 or via email at relia1 at earthlink.net If you are interested in positions in other areas of the U.S. please contact me as well. I have clients nationwide. I will keep your resume confidential and I won?t release it to anyone without your permission. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From MLashus at pathgroup.com Tue Aug 28 12:08:28 2018 From: MLashus at pathgroup.com (Mighnon Lashus) Date: Tue, 28 Aug 2018 17:08:28 +0000 Subject: [Histonet] Job Opening Message-ID: Any one is in histoland interested in a job opportunity in Chattanooga, TN? It is a 3rd shift position which includes a $4.50 shift differential. Thanks, Mighnon Lashus, HT (ASCP) Laboratory Manager PathGroup 4071 S. Access Rd, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus at pathgroup.com Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Chief Privacy Officer at PathGroup immediately at 615-562-9255. Thank you From patpxs at gmail.com Tue Aug 28 19:53:22 2018 From: patpxs at gmail.com (P Sicurello) Date: Tue, 28 Aug 2018 17:53:22 -0700 Subject: [Histonet] Are you a Histotech working in California? Message-ID: Good Evening Listers, I'm trying to find out how many histotechs there are in California, certified or not, clinical or research. I'm trying to gather numbers to present to the state in an effort to get us licensed by the state. It's an uphill battle but we have to start somewhere. Please reply to me with your response. HT? HTL? No certification? HT (CM)? HTL (CM)? Clinical? Research? Thank you. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 9300 Campus Point Drive La Jolla, CA 92037 858-249-5610 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From Blanca.Lopez at UTSouthwestern.edu Wed Aug 29 08:11:16 2018 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Wed, 29 Aug 2018 13:11:16 +0000 Subject: [Histonet] osmium tetroxide Message-ID: Histotechs: I am looking a protocol to stain osmium tetroxide....Is anybody can share their thoughts...on a frozen section. I attached an idea of how this person wants me to follow his protocol with this staining but I kind of lost... my PI request: I'll be perfusing the mice with saline solution 0.9% followed by 10% formalin Cryoprotect the tissue with 30% sucrose Cut 30 micron sections in cryostat and mount on slides I would handle you the slides, since the tissue contains Fluorescent proteins, must be protected from light Once the staining with osmium is finished, you can give me the slides back to check the staining and the fluorescence before it is dehydrated and coverslip. thanks for your help Blanca Lopez HT (ASCP) Histotechnologist UT Southwestern Medical Center Harold C. Simmons Comprehensive Cancer Center Histology Lab K1-210 214-648-7598 blanca.lopez at utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. From lmarie08 at uga.edu Wed Aug 29 09:26:29 2018 From: lmarie08 at uga.edu (Lauren Sweeney) Date: Wed, 29 Aug 2018 14:26:29 +0000 Subject: [Histonet] xylene recycling question Message-ID: Hi all you fellow Histonetters! My query of the day to you is: can you recycle xylene that has been contaminated with formalin? (yes, you heard me correctly, formalin) Thanks for any insight! L From Shannon.Logan at bellin.org Wed Aug 29 09:49:16 2018 From: Shannon.Logan at bellin.org (Logan, Shannon) Date: Wed, 29 Aug 2018 14:49:16 +0000 Subject: [Histonet] osmium tetroxide In-Reply-To: References: Message-ID: <764402becc904be68607f747b7a40717@BAPWEXCH001b.bellin.com> Osmium Tetroxide is highly toxic. Storage is an issue and working with it, you must be under a hood with eye protection. I think most (if not all) labs have banned this substance? If you do not ?have? to work with this material, I would recommend avoiding it. Just my opinion, thanks? Shannon H. Logan B.S., HTL (ASCP) Pathology Department Bellin Health Memorial Hospital 744 South Webster Avenue Green Bay, WI 54305-3400 920-433-3653 X3727 From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, August 29, 2018 8:11 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] osmium tetroxide CAUTION: This email originated from outside of Bellin. Do not click on any links or open any attachments unless you recognize the sender and know the contents are safe. Questions? Contact the Service Desk @ x258600 or (920) 436-8600 Histotechs: I am looking a protocol to stain osmium tetroxide....Is anybody can share their thoughts...on a frozen section. I attached an idea of how this person wants me to follow his protocol with this staining but I kind of lost... my PI request: I'll be perfusing the mice with saline solution 0.9% followed by 10% formalin Cryoprotect the tissue with 30% sucrose Cut 30 micron sections in cryostat and mount on slides I would handle you the slides, since the tissue contains Fluorescent proteins, must be protected from light Once the staining with osmium is finished, you can give me the slides back to check the staining and the fluorescence before it is dehydrated and coverslip. thanks for your help Blanca Lopez HT (ASCP) Histotechnologist UT Southwestern Medical Center Harold C. Simmons Comprehensive Cancer Center Histology Lab K1-210 214-648-7598 blanca.lopez at utsouthwestern.edu> ________________________________ UT Southwestern Medical Center The future of medicine, today. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hhawkins at UTMB.EDU Wed Aug 29 10:07:39 2018 From: hhawkins at UTMB.EDU (Hawkins, Hal K.) Date: Wed, 29 Aug 2018 15:07:39 +0000 Subject: [Histonet] osmium tetroxide In-Reply-To: <764402becc904be68607f747b7a40717@BAPWEXCH001b.bellin.com> References: , <764402becc904be68607f747b7a40717@BAPWEXCH001b.bellin.com> Message-ID: <22624908330375439D6382C9F95093FF9C8E2EBC@GRMBX1.utmb.edu> I would suspect osmium would destroy the fluorescent proteins the PI wants to detect, while staining fat droplets nicely. You can always ask your friendly EM lab to do the staining to find out, they have to use it and are familiar with the proper ways to deal with it. ________________________________________ From: Logan, Shannon via Histonet [histonet at lists.utsouthwestern.edu] Sent: Wednesday, August 29, 2018 9:49 AM To: Blanca Lopez Cc: histonet (histonet at lists.utsouthwestern.edu) Subject: Re: [Histonet] osmium tetroxide WARNING: This email originated from outside of UTMB's email system. Do not click links or open attachments unless you recognize the sender and know the content is safe. Osmium Tetroxide is highly toxic. Storage is an issue and working with it, you must be under a hood with eye protection. I think most (if not all) labs have banned this substance? If you do not ?have? to work with this material, I would recommend avoiding it. Just my opinion, thanks? Shannon H. Logan B.S., HTL (ASCP) Pathology Department Bellin Health Memorial Hospital 744 South Webster Avenue Green Bay, WI 54305-3400 920-433-3653 X3727 From: Blanca Lopez via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, August 29, 2018 8:11 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] osmium tetroxide CAUTION: This email originated from outside of Bellin. Do not click on any links or open any attachments unless you recognize the sender and know the contents are safe. Questions? Contact the Service Desk @ x258600 or (920) 436-8600 Histotechs: I am looking a protocol to stain osmium tetroxide....Is anybody can share their thoughts...on a frozen section. I attached an idea of how this person wants me to follow his protocol with this staining but I kind of lost... my PI request: I'll be perfusing the mice with saline solution 0.9% followed by 10% formalin Cryoprotect the tissue with 30% sucrose Cut 30 micron sections in cryostat and mount on slides I would handle you the slides, since the tissue contains Fluorescent proteins, must be protected from light Once the staining with osmium is finished, you can give me the slides back to check the staining and the fluorescence before it is dehydrated and coverslip. thanks for your help Blanca Lopez HT (ASCP) Histotechnologist UT Southwestern Medical Center Harold C. Simmons Comprehensive Cancer Center Histology Lab K1-210 214-648-7598 blanca.lopez at utsouthwestern.edu> ________________________________ UT Southwestern Medical Center The future of medicine, today. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Chhawkins%40utmb.edu%7C60c2a046a0ca4b341dc408d60dbeabf6%7C7bef256d85db4526a72d31aea2546852%7C0%7C0%7C636711509916930918&sdata=vtXeSADnRqbmQwuxIebXUZW5EWQq%2Fck9%2Bwyku7uFjcg%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=02%7C01%7Chhawkins%40utmb.edu%7C60c2a046a0ca4b341dc408d60dbeabf6%7C7bef256d85db4526a72d31aea2546852%7C0%7C0%7C636711509916930918&sdata=vtXeSADnRqbmQwuxIebXUZW5EWQq%2Fck9%2Bwyku7uFjcg%3D&reserved=0 From relia1 at earthlink.net Wed Aug 29 12:41:20 2018 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 29 Aug 2018 13:41:20 -0400 Subject: [Histonet] Have a Safe and Happy Labor Day Weekend! Message-ID: <000001d43fbf$7f891260$7e9b3720$@earthlink.net> Hello Histonetters, It is hard to believe the summer is almost over. The kids are going back to school and Labor Day is just a few days away! In a few more weeks -Halloween; Then Thanksgiving, Christmas and New Year's Day!! Looking for a new job in the Fall? Planning a job change after the holidays? Considering making a move in 2019? Let's Get The Ball Rolling!!! Histonetters! **Are you a histo tech looking for a something better? **Are you a new or recent graduate of a histology school? **Are you a traveler considering switch into a permanent position? **Are you a Histotech looking to advance into leadership? **Are you a Supervisor looking to advance to lab management? Let's Get The Ball Rolling!!! All you have to do is contact me at relia1 at earthlink.net or call me at 866-607-3542 my office is open M-F 7am-8pm EST or by appointment. If you know of anyone else who might be interested in subscribing to RELIA's Histology Careers Bulletin please feel free to pass this along to them. Here is some information about my current openings: Histology Managers/Supervisors needed in: North Carolina - Histology Lab Manager - A RELIA Exclusive!! Colorado - Histology Lab Manager- A RELIA Exclusive!! California - Histology Sales Support Specialist Histotechnicians/Histotechnologists needed in: MO - Histotech - nights Kansas City TN - Histotech - nights Chattanooga $$ shift diff! NC - Histotechnician/Histotechnologist - days Fayetteville PA - Histology Tech - DAYS Pottstown NY - Histotech - Rye Brook -evenings AL - Dermpath Histotech evenings - Birmingham IN - Histology Tech nights - Hammond, IN (sign on bonus)! Remember it never hurts to look!!! Thanks, Pam 877-60 RELIA (877-607-3542) Cell/Text (407)353-5070 I know there are a lot of recruiters out there right now contacting you and your friends. RELIA is the only nationwide recruiting firm specializing in the permanent placement of histology professionals. Remember here at RELIA we work as your confidential advocate to help you make the move that is right for you when the time is right for you. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From carl.hobbs at kcl.ac.uk Wed Aug 29 14:49:37 2018 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Wed, 29 Aug 2018 19:49:37 +0000 Subject: [Histonet] osmium tetroxide Message-ID: Please ask why Osmium is required? Good advice given so far. If it is to "stain" for myelin, surely an anti Myelin antibody should be used? Visualise using a complementary Fluorophore to that which is indicated but not explained, in your request? Good luck Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6813 From patpxs at gmail.com Wed Aug 29 18:15:23 2018 From: patpxs at gmail.com (P Sicurello) Date: Wed, 29 Aug 2018 16:15:23 -0700 Subject: [Histonet] Unstained Slides - results Message-ID: Good Afternoon Listers, Thanks to all who answered my question about retention of unstained slides. I had several people weigh in on the topic and we have created an unstained slide policy based on their advice and the literature that was kindly sent to me. The consensus is antigenicity degrades depending on storage conditions, among other things, which can lead to false negative results (antibody dependent - but not worth the risk). We now will keep unstained slides which have been stored at room temperature no longer than 12 weeks. One really good suggestion that will are implementing is if a pathologist wants the slides kept longer than 12 weeks, they get to keep the slides in their possession. We will enter a note in our LIS stating who has the unstained slides. The wisdom of my electronic colleagues comes through once again. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From sandra.cheasty at wisc.edu Thu Aug 30 08:40:14 2018 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Thu, 30 Aug 2018 13:40:14 +0000 Subject: [Histonet] IHC Stainer Message-ID: Hello all, We are in the market for a new IHC stainer. (Our Lab Vision 720 is no longer reliable.) We average about 20 slides a day, but sometimes get research projects that are 50+ slides. Not interested in a closed system; it is for a veterinary histology lab, and we need the flexibility to tweak antibodies for dogs, cats, horses, and zoo animals. I'd really appreciate feedback from users who are in a similar lab setting. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From rjbuesa at yahoo.com Thu Aug 30 09:01:16 2018 From: rjbuesa at yahoo.com (Rene J Buesa) Date: Thu, 30 Aug 2018 14:01:16 +0000 (UTC) Subject: [Histonet] IHC Stainer In-Reply-To: References: Message-ID: <585606248.900411.1535637676466@mail.yahoo.com> Get a DAKO IHC stainer (even if it is "refurbished")Ren? On Thursday, August 30, 2018 9:56 AM, Sandra Cheasty via Histonet wrote: Hello all, ? ? ? ? ? ? ? ? We are in the market for a new IHC stainer. (Our Lab Vision 720 is no longer reliable.) We average about 20 slides a day, but sometimes get research projects that are 50+ slides.? Not interested in a closed system; it is for a veterinary histology lab, and we need the flexibility to tweak antibodies for dogs, cats, horses, and zoo animals. I'd really appreciate feedback from users who are in a similar lab setting. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet | | Virus-free. www.avast.com | From jkempf80 at uga.edu Thu Aug 30 12:58:52 2018 From: jkempf80 at uga.edu (Jennifer Kempf) Date: Thu, 30 Aug 2018 17:58:52 +0000 Subject: [Histonet] IHC Stainer In-Reply-To: References: Message-ID: Biocare Medical Intellipath...its completely open and is a continuous load machine, meaning you can start a run on one rack while still loading programs on the other racks if you choose. Biocare is coming out with a new machine in January (I think), that is supposed to do heat retrieval online as well, instead of in the pressure cooker, but I haven't seen it in action yet. -----Original Message----- From: Sandra Cheasty [mailto:sandra.cheasty at wisc.edu] Sent: Thursday, August 30, 2018 9:40 AM To: Histonet (histonet at lists.utsouthwestern.edu) Subject: [Histonet] IHC Stainer Hello all, We are in the market for a new IHC stainer. (Our Lab Vision 720 is no longer reliable.) We average about 20 slides a day, but sometimes get research projects that are 50+ slides. Not interested in a closed system; it is for a veterinary histology lab, and we need the flexibility to tweak antibodies for dogs, cats, horses, and zoo animals. I'd really appreciate feedback from users who are in a similar lab setting. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From cforster at umn.edu Thu Aug 30 13:11:14 2018 From: cforster at umn.edu (Colleen Forster) Date: Thu, 30 Aug 2018 13:11:14 -0500 Subject: [Histonet] IHC Stainer In-Reply-To: References: Message-ID: I am with Jennifer on the IntelliPath.....I have just gotten 2 of them in my lab. The new instrument was demoed at NSH last year. It is an amazing machine,....I wish I could have had that one VEry limited numbers will be avaiable in November with the main stream sales beginning in January. Colleen Forster HT(ASCP)QIHC U of MN On Thu, Aug 30, 2018 at 12:58 PM, Jennifer Kempf via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Biocare Medical Intellipath...its completely open and is a continuous load > machine, meaning you can start a run on one rack while still loading > programs on the other racks if you choose. Biocare is coming out with a > new machine in January (I think), that is supposed to do heat retrieval > online as well, instead of in the pressure cooker, but I haven't seen it in > action yet. > > -----Original Message----- > From: Sandra Cheasty [mailto:sandra.cheasty at wisc.edu] > Sent: Thursday, August 30, 2018 9:40 AM > To: Histonet (histonet at lists.utsouthwestern.edu) utsouthwestern.edu> > Subject: [Histonet] IHC Stainer > > Hello all, > We are in the market for a new IHC stainer. (Our Lab > Vision 720 is no longer reliable.) We average about 20 slides a day, but > sometimes get research projects that are 50+ slides. Not interested in a > closed system; it is for a veterinary histology lab, and we need the > flexibility to tweak antibodies for dogs, cats, horses, and zoo animals. > I'd really appreciate feedback from users who are in a similar lab setting. > > Cheers! > Sandy > > Sandra J. Cheasty, HT (ASCP) > Histology & Necropsy Supervisor > UW-Madison, School of Veterinary Medicine > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at holml at umn.edu * From PKRichar at gundersenhealth.org Thu Aug 30 16:25:55 2018 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Thu, 30 Aug 2018 21:25:55 +0000 Subject: [Histonet] Cytology workload Message-ID: <998284C32F61104CA0BEFFFFCF6F90FDE510ABFD@LXEXMB01.gundluth.org> Hi, I am wondering if anyone would be able to help me with Cytology workload expectations. Cordially, Pam ~ National Histology Professionals Day 3/10/19 Pathologist Assistant Day 4/14/2019 Medical Laboratory Professionals Week April 21-27, 2019 National Cytotechnology Day 5/13/201* +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org