From dgoodwin at princetonhcs.org Fri Sep 1 09:16:23 2017 From: dgoodwin at princetonhcs.org (goodwin, diana) Date: Fri, 1 Sep 2017 14:16:23 +0000 Subject: [Histonet] Multi-stainers Message-ID: Good Morning! Happy Labor Day Weekend - hope you have something fun planned. I am soliciting opinions re: H&E stainers. What are you using and what are the pros and cons? Focus is on through-put (turn-around time) with multiple protocols loaded. Regards, Diana Goodwin UMCPP Anatomic Pathology Supervisor Ph: 609-853-6808 Fax: 609-853-6841 This e-mail transmission and any documents attached hereto contain information from Princeton HealthCare System which is confidential and/or legally privileged. This information is intended only for the use of the individual or entity to which it is addressed, and only for the purpose for which this transmission was made. 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From julio.benavides.silvan at csic.es Fri Sep 1 09:28:37 2017 From: julio.benavides.silvan at csic.es (Julio Benavides) Date: Fri, 1 Sep 2017 16:28:37 +0200 Subject: [Histonet] comparison between microtomes leica RM 2125RTS y thermo HM325 In-Reply-To: <43D06FD6-D6EE-4659-934B-AB4AED6BA132@yahoo.com> References: <43D06FD6-D6EE-4659-934B-AB4AED6BA132@yahoo.com> Message-ID: Hi there, we are buying a new microtome for the lab (research, not high load of work) and have been offered leica RM 2125RTS and thermo HM325 with similar prices. Could anyone help us to decide? thanks a lot cheers Julio From jaylundgren at gmail.com Fri Sep 1 12:08:14 2017 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 1 Sep 2017 12:08:14 -0500 Subject: [Histonet] comparison between microtomes leica RM 2125RTS y thermo HM325 In-Reply-To: References: <43D06FD6-D6EE-4659-934B-AB4AED6BA132@yahoo.com> Message-ID: I prefer the Leica, because it has that nifty rough cut button, but that's just my personal choice. The Thermo has a better waste tray. I think you'd be happy with either one. The best thing for you to do would be to get your sales reps to bring them both to your lab to demo and pick your favorite. Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Sep 1, 2017 at 9:28 AM, Julio Benavides via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > Hi there, > > we are buying a new microtome for the lab (research, not high load of > work) and have been offered leica RM 2125RTS and thermo HM325 with similar > prices. > > Could anyone help us to decide? > > thanks a lot > > cheers > > Julio > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From deb.vaneyck at phci.org Fri Sep 1 12:12:56 2017 From: deb.vaneyck at phci.org (Van Eyck, Deb) Date: Fri, 1 Sep 2017 17:12:56 +0000 Subject: [Histonet] HSV I & II Message-ID: Hi All, Does anyone know what the issue is, all of the sudden the HSV 1 and 2 stains are no longer available from vendors -thanks Deb ______________________________________________________________________ This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. ______________________________________________________________________ From bcooper at chla.usc.edu Fri Sep 1 13:26:53 2017 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Fri, 1 Sep 2017 18:26:53 +0000 Subject: [Histonet] Need help identifying a product!!! Message-ID: Happy Friday Histonet! Our cassette printed died this week, and we've been handwriting cassettes for the last couple of days. It's been YEARS since I had to do this; BOY has my handwriting atrophied since then! I remember we used to have this little metal rack that held the cassettes perfectly stationary when you wrote on them; the rack held about 20-25 cassettes in 4 or 5 rows. Do any of you remember these? I've been searching online for a couple days, but Google and all the lab supply sites just aren't catching what I'm looking for. Our printer will be fixed in a few hours, but just in case, I'd like to have one of these racks for the future. Any ideas? Should I check antique shops? :) Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 Pager: 213-209-0184 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From paula at excaliburpathology.com Fri Sep 1 13:41:29 2017 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Fri, 1 Sep 2017 18:41:29 +0000 (UTC) Subject: [Histonet] Need help identifying a product!!! In-Reply-To: References: Message-ID: <145659811.1177577.1504291289272@mail.yahoo.com> Have you tried MarketLab? They have things like that.?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: "Cooper, Brian via Histonet" To: "histonet at lists.utsouthwestern.edu" Sent: Friday, September 1, 2017 1:34 PM Subject: [Histonet] Need help identifying a product!!! Happy Friday Histonet! Our cassette printed died this week, and we've been handwriting cassettes for the last couple of days.? It's been YEARS since I had to do this; BOY has my handwriting atrophied since then!? I remember we used to have this little metal rack that held the cassettes perfectly stationary when you wrote on them; the rack held about 20-25 cassettes in 4 or 5 rows.? Do any of you remember these?? I've been searching online for a couple days, but Google and all the lab supply sites just aren't catching what I'm looking for.? Our printer will be fixed in a few hours, but just in case, I'd like to have one of these racks for the future.? Any ideas?? Should I check antique shops?? :) Thanks, Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357? ? Pager: 213-209-0184 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message.? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From p_umang at hotmail.com Fri Sep 1 13:56:58 2017 From: p_umang at hotmail.com (Umang Pandya) Date: Fri, 1 Sep 2017 18:56:58 +0000 Subject: [Histonet] comparison between microtomes leica RM 2125RTS y thermo HM325 In-Reply-To: References: <43D06FD6-D6EE-4659-934B-AB4AED6BA132@yahoo.com> , Message-ID: Also try Sakura autoSection. It has auto align features for the block to the slide. No need to worry about trimming your precious sample and loosing them. I would suggest a demo from Sakura as well. ________________________________ From: Jay Lundgren via Histonet Sent: Friday, September 1, 2017 10:08 AM To: Julio Benavides Cc: histonet Subject: Re: [Histonet] comparison between microtomes leica RM 2125RTS y thermo HM325 I prefer the Leica, because it has that nifty rough cut button, but that's just my personal choice. The Thermo has a better waste tray. I think you'd be happy with either one. The best thing for you to do would be to get your sales reps to bring them both to your lab to demo and pick your favorite. Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Sep 1, 2017 at 9:28 AM, Julio Benavides via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > Hi there, > > we are buying a new microtome for the lab (research, not high load of > work) and have been offered leica RM 2125RTS and thermo HM325 with similar > prices. > > Could anyone help us to decide? > > thanks a lot > > cheers > > Julio > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald at mtsac.edu Fri Sep 1 16:26:00 2017 From: JMacDonald at mtsac.edu (Jennifer MacDonald) Date: Fri, 1 Sep 2017 14:26:00 -0700 Subject: [Histonet] comparison between microtomes leica RM 2125RTS y thermo HM325 In-Reply-To: References: <43D06FD6-D6EE-4659-934B-AB4AED6BA132@yahoo.com> Message-ID: The HM325 also has that feature. From: Jay Lundgren via Histonet To: Julio Benavides Cc: histonet Date: 09/01/2017 10:11 AM Subject: Re: [Histonet] comparison between microtomes leica RM 2125RTS y thermo HM325 I prefer the Leica, because it has that nifty rough cut button, but that's just my personal choice. The Thermo has a better waste tray. I think you'd be happy with either one. The best thing for you to do would be to get your sales reps to bring them both to your lab to demo and pick your favorite. Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Sep 1, 2017 at 9:28 AM, Julio Benavides via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > Hi there, > > we are buying a new microtome for the lab (research, not high load of > work) and have been offered leica RM 2125RTS and thermo HM325 with similar > prices. > > Could anyone help us to decide? > > thanks a lot > > cheers > > Julio > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From steve8438 at gmail.com Fri Sep 1 18:34:02 2017 From: steve8438 at gmail.com (Steve Mello) Date: Fri, 01 Sep 2017 19:34:02 -0400 Subject: [Histonet] comparison between microtomes leica RM 2125RTS y thermo HM325 In-Reply-To: References: <43D06FD6-D6EE-4659-934B-AB4AED6BA132@yahoo.com> Message-ID: <20170901233402.4653134.59748.80957@gmail.com> I prefer the quality and mechanics of the Leica vs Thermo. But, clearly it would be what you feel comfortable using. Also, service is,in my opinion, better with Leica for response times.? Steven Mello, HT(ASCP) Sent?from?my?BlackBerry?10?smartphone. ? Original Message ? From: Jennifer MacDonald via Histonet Sent: Friday, September 1, 2017 5:27 PM To: Jay Lundgren Reply To: Jennifer MacDonald Cc: histonet Subject: Re: [Histonet] comparison between microtomes leica RM 2125RTS y thermo HM325 The HM325 also has that feature. From: Jay Lundgren via Histonet To: Julio Benavides Cc: histonet Date: 09/01/2017 10:11 AM Subject: Re: [Histonet] comparison between microtomes leica RM 2125RTS y thermo HM325 I prefer the Leica, because it has that nifty rough cut button, but that's just my personal choice. The Thermo has a better waste tray. I think you'd be happy with either one. The best thing for you to do would be to get your sales reps to bring them both to your lab to demo and pick your favorite. Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Sep 1, 2017 at 9:28 AM, Julio Benavides via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > Hi there, > > we are buying a new microtome for the lab (research, not high load of > work) and have been offered leica RM 2125RTS and thermo HM325 with similar > prices. > > Could anyone help us to decide? > > thanks a lot > > cheers > > Julio > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren at gmail.com Mon Sep 4 01:58:57 2017 From: jaylundgren at gmail.com (Jay Lundgren) Date: Mon, 4 Sep 2017 01:58:57 -0500 Subject: [Histonet] Need help identifying a product!!! In-Reply-To: <145659811.1177577.1504291289272@mail.yahoo.com> References: <145659811.1177577.1504291289272@mail.yahoo.com> Message-ID: It was a statlab thingy, I 'member that much. On Fri, Sep 1, 2017 at 1:41 PM, Paula Keene Pierce via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Have you tried MarketLab? They have things like that. Paula Keene Pierce, > BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake > DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www. > excaliburpathology.com > > From: "Cooper, Brian via Histonet" utsouthwestern.edu> > To: "histonet at lists.utsouthwestern.edu" utsouthwestern.edu> > Sent: Friday, September 1, 2017 1:34 PM > Subject: [Histonet] Need help identifying a product!!! > > Happy Friday Histonet! > > Our cassette printed died this week, and we've been handwriting cassettes > for the last couple of days. It's been YEARS since I had to do this; BOY > has my handwriting atrophied since then! I remember we used to have this > little metal rack that held the cassettes perfectly stationary when you > wrote on them; the rack held about 20-25 cassettes in 4 or 5 rows. Do any > of you remember these? I've been searching online for a couple days, but > Google and all the lab supply sites just aren't catching what I'm looking > for. Our printer will be fixed in a few hours, but just in case, I'd like > to have one of these racks for the future. Any ideas? Should I check > antique shops? :) > > Thanks, > > Brian D. Cooper, HT (ASCP)CM | Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 Pager: 213-209-0184 > bcooper at chla.usc.edu > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain > confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, > please > contact the sender by reply e-mail and destroy all copies of this original > message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ronald.kusters at pfmmedical.com Wed Sep 6 01:16:18 2017 From: ronald.kusters at pfmmedical.com (Kusters, Ronald) Date: Wed, 6 Sep 2017 06:16:18 +0000 Subject: [Histonet] comparison between microtomes leica RM 2125RTS y thermo HM325 In-Reply-To: References: <43D06FD6-D6EE-4659-934B-AB4AED6BA132@yahoo.com> Message-ID: <12de86f7a1634488ab8319464c576a7c@EX-PFM-103-W.pfm-ag.net> Dear Julio, You can also look at the pfm Rotary 3004-M (Manual). You may contact Cancer Diagnostics for more info or demo. We also will be at NSH showing our microtomes at the Cancer Diagnostics Booth and hope to meet you there Thank you for looking at https://www.pfmmedical.com/en/productcatalogue/rotary_microtomes/pfm_rotary_3004_m/index.html Ronald Mit freundlichen Gr??en/Kind Regards Ronald Kusters International Sales Manager Feather Brand Histotechnology pfm medical ag Wankelstra?e 60 50996 K?ln, Germany T +49 (2236) 9641 660 F +49 (2236) 9641 99 660 M +49 (171) 9144343 -----Original Message----- From: Julio Benavides [mailto:julio.benavides.silvan at csic.es] Sent: vrijdag 1 september 2017 16:29 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] comparison between microtomes leica RM 2125RTS y thermo HM325 Hi there, we are buying a new microtome for the lab (research, not high load of work) and have been offered leica RM 2125RTS and thermo HM325 with similar prices. Could anyone help us to decide? thanks a lot cheers Julio From CMcgrad1 at hurleymc.com Wed Sep 6 09:17:46 2017 From: CMcgrad1 at hurleymc.com (Cynthia McGrady) Date: Wed, 6 Sep 2017 14:17:46 +0000 Subject: [Histonet] cat scratch Message-ID: <0053E7F9DD068946AC3541D0A35C098A0206E4C2B2@ExchangeMB3.hmc.hurleymc.com> Anyone out there have a source for cat scratch control block or slides? Any information would be greatly appreciated. Thanks.. Cindy McGrady From SanderBD at EVMS.EDU Wed Sep 6 10:04:41 2017 From: SanderBD at EVMS.EDU (Sanders, Brie D.) Date: Wed, 6 Sep 2017 15:04:41 +0000 Subject: [Histonet] Histology Technician job opening Message-ID: <2204B14F79ED0148A6E298DEDA6D6411CD2317@Trillya.evms.net> Hello, Eastern Virginia Medical School has an opening for a Histology Technician in Norfolk, Virginia. The link to apply is as follows: https://careers-evms.icims.com/jobs/2292/histology-technician/job?mode=view&mobile=false&width=784&height=500&bga=true&needsRedirect=false&jan1offset=-300&jun1offset=-240 EVMS is an Equal Opportunity/Affirmative Action Employer of Minorities, Females, Individuals with Disabilities, Protected Veterans, and Drug and Tobacco Free workplace. ________________________________ Brie Sanders Employment Specialist *: 757-446-6043 7: 757-446-6135 | *sanderbd at evms.edu Eastern Virginia Medical School | Human Resources | WWW.EVMS.EDU | 358 Mowbray Arch | Suite 201 | Norfolk, VA 23507 Community Focus. World Impact From foreightl at gmail.com Wed Sep 6 10:42:56 2017 From: foreightl at gmail.com (Patrick Laurie) Date: Wed, 6 Sep 2017 11:42:56 -0400 Subject: [Histonet] HSV I & II In-Reply-To: References: Message-ID: Many antibodies on the market are produced by one company and distributed through several others. From what I have heard the HSV 2 rabbit died at the production company. Could be just a rumor, but I remember this happening with the PAX-2 antibody several years ago. It takes quite a while to re-immunize and properly validate the antibody. HSV 2 could be in short supply for a while. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Fri, Sep 1, 2017 at 1:12 PM, Van Eyck, Deb via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi All, > Does anyone know what the issue is, all of the sudden the HSV 1 and 2 > stains are no longer available from vendors -thanks Deb > > > > ______________________________________________________________________ > > This information is confidential and intended solely for the use of the > individual or entity to whom it is addressed. If you have received this > email in error please notify the sender or our Customer Support Center at (262) > 928-2777. We have scanned this e-mail and its attachments for malicious > content. However, the recipient should check this email and any attachments > for the presence of viruses. ProHealth Care accepts no liability for any > damage caused by any virus transmitted by this email. > ______________________________________________________________________ > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From deb.vaneyck at phci.org Wed Sep 6 10:47:12 2017 From: deb.vaneyck at phci.org (Van Eyck, Deb) Date: Wed, 6 Sep 2017 15:47:12 +0000 Subject: [Histonet] HSV I & II In-Reply-To: References: Message-ID: This is also what I suspect from some of the comments being made to me by Vendors , many are not saying but I heard that one of my vendors that they are working up a new antibody. S0 we may all have to revalidate if using the same clone, after a wait. Didn?t that happen to the CD 3 rabbit years ago. Deb Are there any rabbit monoclonals out there now? From: Patrick Laurie [mailto:foreightl at gmail.com] Sent: Wednesday, September 06, 2017 10:43 AM To: Van Eyck, Deb Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] HSV I & II Many antibodies on the market are produced by one company and distributed through several others. From what I have heard the HSV 2 rabbit died at the production company. Could be just a rumor, but I remember this happening with the PAX-2 antibody several years ago. It takes quite a while to re-immunize and properly validate the antibody. HSV 2 could be in short supply for a while. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Fri, Sep 1, 2017 at 1:12 PM, Van Eyck, Deb via Histonet > wrote: Hi All, Does anyone know what the issue is, all of the sudden the HSV 1 and 2 stains are no longer available from vendors -thanks Deb ______________________________________________________________________ This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. ______________________________________________________________________ From Blanca.Lopez at UTSouthwestern.edu Wed Sep 6 11:22:17 2017 From: Blanca.Lopez at UTSouthwestern.edu (Blanca Lopez) Date: Wed, 6 Sep 2017 16:22:17 +0000 Subject: [Histonet] PIG processing tissue help Message-ID: <755f58cda1c04fdb9372d489516c020a@SWMS13MAIL12.swmed.org> Hello Histonettes, I am looking for a better information on what is the best way to treat, fix and process pig heart. This is a very new thing for me because I usually work with mouse and human tissue. Is anybody there that can help me with this please? I will appreciate any feedback event to do perform special stains and IHC for this project, Thanks ________________________________ UT Southwestern Medical Center The future of medicine, today. From ewj at pigs.ag Wed Sep 6 15:29:24 2017 From: ewj at pigs.ag (E. Wayne johnson) Date: Thu, 7 Sep 2017 04:29:24 +0800 Subject: [Histonet] PIG processing tissue In-Reply-To: References: Message-ID: We routinely examine pig heart as part of diagnostic histopathology and generally we are looking for the lesions of Vitamin E / Selenium deficiency. Vitamin E and organic Se are expensive and often deficient in commercial piglet diets. We simply fix with a formalin based fixative and process routinely to paraffin and stain with our H & E. - E. Wayne Johnson Enable AgTech, Beijing. "Never do anything mean to a pig.? They'll squeal on you every time." From kiran_g at sbcglobal.net Wed Sep 6 19:18:38 2017 From: kiran_g at sbcglobal.net (Kiran) Date: Wed, 6 Sep 2017 17:18:38 -0700 Subject: [Histonet] IHC stains on Cytology specimens Message-ID: <995A3712-01E2-40B3-9360-0AE319E9D9C8@sbcglobal.net> Dear HistologyExperts, I am looking for anyone who is running IHC stains on Cytology specimens ( e.g SurePath ). We need some guidance on method validation and a lab to send out for comparative studies. Thanks, Kiran From michael.gudo at morphisto.de Thu Sep 7 01:40:55 2017 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Thu, 7 Sep 2017 08:40:55 +0200 Subject: [Histonet] PIG processing tissue help In-Reply-To: <755f58cda1c04fdb9372d489516c020a@SWMS13MAIL12.swmed.org> References: <755f58cda1c04fdb9372d489516c020a@SWMS13MAIL12.swmed.org> Message-ID: <5F444634-4A95-4257-984E-3BA779AC9A1E@morphisto.de> Hello, we process heart and other biopsies of muscles in Bouin oder Bouin-Hollande fixative which both also work fine with IHC and give very brilliant results with any kind of trichrome stainings. With best regards Michael > Am 06.09.2017 um 18:22 schrieb Blanca Lopez via Histonet : > > Hello Histonettes, > I am looking for a better information on what is the best way to treat, fix and process pig heart. This is a very new thing for me because I usually work with mouse and human tissue. Is anybody there that can help me with this please? I will appreciate any feedback event to do perform special stains and IHC for this project, > Thanks > > ________________________________ > > UT Southwestern > > > Medical Center > > > > The future of medicine, today. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO Evolutionsforschung und Anwendung GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. 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From carlos.torresvega at gmail.com Thu Sep 7 07:41:09 2017 From: carlos.torresvega at gmail.com (Carlos Torres Vega) Date: Thu, 7 Sep 2017 07:41:09 -0500 Subject: [Histonet] Software for leica Ip S slide printer Message-ID: <13507910-2E3B-411A-98D9-5819BED85F6F@gmail.com> Hi there ho the whole Histonet comunity I recently bougth an IP S slide printer second hand for my lab but I need the leica software I asked directly to the leica biosystems page but no response. Does any body have it and could let me a copy Thanks for your help Atte Dr carlos torres vega Carlos.torresvega at gmail.com Morelia mich mexico Universidad Michoacana Enviado desde mi iPhone From patpxs at gmail.com Thu Sep 7 15:55:18 2017 From: patpxs at gmail.com (P Sicurello) Date: Thu, 7 Sep 2017 13:55:18 -0700 Subject: [Histonet] LANA (HHV-8) control tissue? Message-ID: Hello Listers, Does anyone have control tissue for LANA? Perhaps we can trade for something you need. Thanks in advance. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 <#> *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. From mikael.niku at helsinki.fi Fri Sep 8 07:54:13 2017 From: mikael.niku at helsinki.fi (Niku, Mikael) Date: Fri, 8 Sep 2017 12:54:13 +0000 Subject: [Histonet] Are there any broad-spectrum anti-bacterial antibodies? Message-ID: Are there any antibodies that can be used to detect broad groups of bacteria in tissue sections or flow cytometry? Like, ANY bacterium (as opposed to animal cells), or gram+ / gram- bacteria, or a bacterial phylum? Or alternatively: are there any antibodies for mammalian intestinal commensal bacteria, let's say at genus level? With best regards, Mikael Niku University of Helsinki, Finland From Sherrian.McAnn at va.gov Fri Sep 8 09:31:58 2017 From: Sherrian.McAnn at va.gov (McAnn, Sherrian) Date: Fri, 8 Sep 2017 14:31:58 +0000 Subject: [Histonet] please remove me from Histonet Message-ID: From Timothy.Morken at ucsf.edu Fri Sep 8 16:10:31 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 8 Sep 2017 21:10:31 +0000 Subject: [Histonet] EM renal embedding of long cores Message-ID: Hi all, I am interesting anyone's experience EM embedding of long renal cores, say in 5mm increments, rather that dividing to 1 to 2 mm pieces, for blocks, and thick sections. We are wondering about then going on to produce EM thin sections of gloms identified for EM. Is the core re-trimmed to smaller pieces and re-embedded, or some other method? Any info will be appreciated. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From bernardgregoryamparo at gmail.com Mon Sep 11 03:24:33 2017 From: bernardgregoryamparo at gmail.com (Bernard Gregory Amparo) Date: Mon, 11 Sep 2017 11:24:33 +0300 Subject: [Histonet] Protocols for cryostat trimming and sectioning Message-ID: Good day Hisonetters, I would like to ask about your protocol for the cryostats you are using in your lab. We are using a Leica CM1950 and we are now validating the machine for use. So could you please help me to gain some insights regarding this matter? Thanks! From CDavis at che-east.org Mon Sep 11 08:08:08 2017 From: CDavis at che-east.org (Cassie P. Davis) Date: Mon, 11 Sep 2017 13:08:08 +0000 Subject: [Histonet] Billing question help please Message-ID: Good morning Histoland, our supervisor came in asking about an regulation she was told about this morning that says no more than 9 IHC can be billed on a patient per day. Can some one help me find a resource for this? Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: CDavis at che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From patpxs at gmail.com Mon Sep 11 09:35:04 2017 From: patpxs at gmail.com (P Sicurello) Date: Mon, 11 Sep 2017 07:35:04 -0700 Subject: [Histonet] Protocols for cryostat trimming and sectioning In-Reply-To: References: Message-ID: Hi Bernard, If memory serves me correctly, a cryostat is like a microtome and does not need validation - CAP requires a function check (look in the All Common Checklist) and verification from the vendor that it was installed correctly and it operates as it should. Does it turn on? Does it get cold? Does it cut? Things like that. I hope that helps. Sincerely, Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. On Mon, Sep 11, 2017 at 1:24 AM, Bernard Gregory Amparo via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good day Hisonetters, > > > > I would like to ask about your protocol for the cryostats you are using in > your lab. We are using a Leica CM1950 and we are now validating the machine > for use. > > So could you please help me to gain some insights regarding this matter? > > > > > > Thanks! > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From amurvosh at advancederm.net Tue Sep 12 14:42:05 2017 From: amurvosh at advancederm.net (Anne Murvosh) Date: Tue, 12 Sep 2017 19:42:05 +0000 Subject: [Histonet] Temp employees Message-ID: <22BDD9AABC13E24E95D1CF064B75C4B7B5A109@Exchange.Advancederm.net> Does anyone know of a company that provides temp employees for a Mohs technician? We need to cover someone for a little over a month in February or March of next year. Thanks Anne Murvosh From isabelsoto1162 at yahoo.com Tue Sep 12 15:06:00 2017 From: isabelsoto1162 at yahoo.com (Isabel Soto) Date: Tue, 12 Sep 2017 20:06:00 +0000 (UTC) Subject: [Histonet] Temp employees In-Reply-To: <22BDD9AABC13E24E95D1CF064B75C4B7B5A109@Exchange.Advancederm.net> References: <22BDD9AABC13E24E95D1CF064B75C4B7B5A109@Exchange.Advancederm.net> Message-ID: <113412349.648600.1505246760442@mail.yahoo.com> Hello... you can call Haleigh Sutherland at 1-801-930-3446 that is CompHealth. Haleigh will take care of you.? Regards,Isabel Soto Sent from Yahoo Mail on Android On Tue, Sep 12, 2017 at 12:50 PM, Anne Murvosh via Histonet wrote: Does anyone know of a company that provides temp employees for a Mohs technician? We need to cover someone for a little over a month in February or March of next year. Thanks Anne Murvosh _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet On Tue, Sep 12, 2017 at 12:50 PM, Anne Murvosh via Histonet wrote: Does anyone know of a company that provides temp employees for a Mohs technician? We need to cover someone for a little over a month in February or March of next year. Thanks Anne Murvosh _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet On Tue, Sep 12, 2017 at 12:50 PM, Anne Murvosh via Histonet wrote: Does anyone know of a company that provides temp employees for a Mohs technician? We need to cover someone for a little over a month in February or March of next year. Thanks Anne Murvosh _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jvickroy at SpringfieldClinic.com Tue Sep 12 15:45:50 2017 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Tue, 12 Sep 2017 20:45:50 +0000 Subject: [Histonet] Formalin collection at grossing stations Message-ID: <9B1A1501A800064397369BD8072E6BCA6D514974@E2K10DB.springfieldclinic.com> We have been told by our chemical waste company that we no longer can discard small biopsy containers with formalin in them. In our workflow process we gross small specimens. We generally use forceps to take the small biopsies from the container of formalin and reseal the container with the residual formalin. The container is then discarded in a red bag and held for two days incase we have to go back for labeling questions. Rarely do we have to go back to make sure a specimen was not left in a container. (example three fragments grossed and the clinician said there were four fragments). Large amounts of formalin from our tissue processors is recycled so the only formalin that has been going in the biohazard waste has been what's in the prefilled containers. However, I know it adds up. We have two options that seem to make the most sense. We can collect and recycle the residual formalin from the containers or we can collect and neutralize the formalin. Since we already recycle it seems logical to recycle when we can however at some point we will have an abundance and will be forced to neutralize. Here is my questions: Do your grossing techs collect the formalin as they gross each specimen or do they leave the containers with the residual formalin in them for a period of time? We will still keep the empty containers for two days whether we dump the formalin while we are grossing or have to go back to them two days later just in case there are labeling questions. If they collect the formalin as they gross each specimen is there any special way they have come up with to keep the formalin fumes down besides removing a lid of a waste container each time they discard the formalin. I realize that grossing is done under the hoods however, I'm not in favor of an open container of formalin in the grossing station. It elevates exposure amounts and expensive filters become exhausted much faster. One of my staff members immediately went into McGiver mode to come up with a method however I'm not sure we aren't overthinking this process. Your thoughts? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From gagnone at KGH.KARI.NET Wed Sep 13 08:44:03 2017 From: gagnone at KGH.KARI.NET (Gagnon, Eric) Date: Wed, 13 Sep 2017 13:44:03 +0000 Subject: [Histonet] FW: Formalin collection at grossing stations In-Reply-To: <9B1A1501A800064397369BD8072E6BCA6D514974@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA6D514974@E2K10DB.springfieldclinic.com> Message-ID: <5F06C3AD0B27264CA20CFA986C87882E01CD84D849@EXCHANGEPV3.KGH.ON.CA> Jim, Our PA's collect formalin after each specimen, dumping it into a waste 10-litre carboy. Most of the waste containers are in a small lab sink beside each grossing area. A funnel positioned in the neck of the carboy not only helps channel the formalin, it also keeps the container mostly closed-off re: fumes. The empty containers are also bagged, and occasionally the bags need to be opened and checked for labelling or other issues that come up. Hope this helps, Eric Gagnon, MLT Histology Laboratory Kingston General Hospital, Kingston, Ontario, Canada ________________________________________ From: Vickroy, James via Histonet [histonet at lists.utsouthwestern.edu] Sent: Tuesday, September 12, 2017 4:45 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Formalin collection at grossing stations We have been told by our chemical waste company that we no longer can discard small biopsy containers with formalin in them. In our workflow process we gross small specimens. We generally use forceps to take the small biopsies from the container of formalin and reseal the container with the residual formalin. The container is then discarded in a red bag and held for two days incase we have to go back for labeling questions. Rarely do we have to go back to make sure a specimen was not left in a container. (example three fragments grossed and the clinician said there were four fragments). Large amounts of formalin from our tissue processors is recycled so the only formalin that has been going in the biohazard waste has been what's in the prefilled containers. However, I know it adds up. We have two options that seem to make the most sense. We can collect and recycle the residual formalin from the containers or we can collect and neutralize the formalin. Since we alr! eady recycle it seems logical to recycle when we can however at some point we will have an abundance and will be forced to neutralize. Here is my questions: Do your grossing techs collect the formalin as they gross each specimen or do they leave the containers with the residual formalin in them for a period of time? We will still keep the empty containers for two days whether we dump the formalin while we are grossing or have to go back to them two days later just in case there are labeling questions. If they collect the formalin as they gross each specimen is there any special way they have come up with to keep the formalin fumes down besides removing a lid of a waste container each time they discard the formalin. I realize that grossing is done under the hoods however, I'm not in favor of an open container of formalin in the grossing station. It elevates exposure amounts and expensive filters become exhausted much faster. One of my staff members immediately went into McGiver mode to come up with a method however I'm not sure we aren't overthinking this process. Your thoughts? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren at gmail.com Wed Sep 13 13:22:23 2017 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 13 Sep 2017 13:22:23 -0500 Subject: [Histonet] Temp employees In-Reply-To: <113412349.648600.1505246760442@mail.yahoo.com> References: <22BDD9AABC13E24E95D1CF064B75C4B7B5A109@Exchange.Advancederm.net> <113412349.648600.1505246760442@mail.yahoo.com> Message-ID: Any of the temp agencies can provide a Mohs tech. On Tue, Sep 12, 2017 at 3:06 PM, Isabel Soto via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello... you can call Haleigh Sutherland at 1-801-930-3446 that is > CompHealth. Haleigh will take care of you. > Regards,Isabel Soto > > Sent from Yahoo Mail on Android > > On Tue, Sep 12, 2017 at 12:50 PM, Anne Murvosh via Histonet< > histonet at lists.utsouthwestern.edu> wrote: Does anyone know of a company > that provides temp employees for a Mohs technician? We need to cover > someone for a little over a month in February or March of next year. Thanks > Anne Murvosh > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > On Tue, Sep 12, 2017 at 12:50 PM, Anne Murvosh via Histonet< > histonet at lists.utsouthwestern.edu> wrote: Does anyone know of a company > that provides temp employees for a Mohs technician? We need to cover > someone for a little over a month in February or March of next year. Thanks > Anne Murvosh > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > On Tue, Sep 12, 2017 at 12:50 PM, Anne Murvosh via Histonet< > histonet at lists.utsouthwestern.edu> wrote: Does anyone know of a company > that provides temp employees for a Mohs technician? We need to cover > someone for a little over a month in February or March of next year. Thanks > Anne Murvosh > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From drbugge at gmail.com Wed Sep 13 17:03:12 2017 From: drbugge at gmail.com (Dawn Bugge) Date: Wed, 13 Sep 2017 15:03:12 -0700 Subject: [Histonet] What is the best microtome? Message-ID: Hello everyone, What is your favorite microtome? I am helping a lab with purchases and I only have experience with the Finesse ME+ and an old Leica. Any thoughts would be great. Thanks. -- Dawn R Bugge From bcooper at chla.usc.edu Wed Sep 13 17:18:36 2017 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 13 Sep 2017 22:18:36 +0000 Subject: [Histonet] What is the best microtome? In-Reply-To: References: Message-ID: Microm HM325 gets my vote. -----Original Message----- From: Dawn Bugge via Histonet [histonet at lists.utsouthwestern.edu] Received: Wednesday, 13 Sep 2017, 3:04PM To: histonet at lists.utsouthwestern.edu [histonet at lists.utsouthwestern.edu] Subject: [Histonet] What is the best microtome? (EXTERNAL EMAIL) Hello everyone, What is your favorite microtome? I am helping a lab with purchases and I only have experience with the Finesse ME+ and an old Leica. Any thoughts would be great. Thanks. -- Dawn R Bugge _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From isabelsoto1162 at yahoo.com Wed Sep 13 17:36:47 2017 From: isabelsoto1162 at yahoo.com (Isabel Soto) Date: Wed, 13 Sep 2017 22:36:47 +0000 (UTC) Subject: [Histonet] What is the best microtome? In-Reply-To: References: Message-ID: <598671377.1544680.1505342207468@mail.yahoo.com> Personally I love microm Sent from Yahoo Mail on Android On Wed, Sep 13, 2017 at 3:29 PM, Cooper, Brian via Histonet wrote: Microm HM325 gets my vote. -----Original Message----- From: Dawn Bugge via Histonet [histonet at lists.utsouthwestern.edu] Received: Wednesday, 13 Sep 2017, 3:04PM To: histonet at lists.utsouthwestern.edu [histonet at lists.utsouthwestern.edu] Subject: [Histonet] What is the best microtome? (EXTERNAL EMAIL) Hello everyone, What is your favorite microtome?? I am helping a lab with purchases and I only have experience with the Finesse ME+ and an old Leica.? Any thoughts would be great. Thanks. -- Dawn R Bugge _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message.? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michael.gudo at morphisto.de Thu Sep 14 01:00:29 2017 From: michael.gudo at morphisto.de (Dr. Michael Gudo) Date: Thu, 14 Sep 2017 08:00:29 +0200 Subject: [Histonet] What is the best microtome? In-Reply-To: References: Message-ID: <5ACF57EE-8402-4BE7-9D6E-97CC883C0555@morphisto.de> We have Leica microtomes in out lab and they work very very good. The components are compatible over several Generations and they work for at least more than 15-20 years! And the best thing is the Waterbath directly attached to the Blade. Greetings Michael Von meinem iPhone gesendet > Am 14.09.2017 um 00:18 schrieb Cooper, Brian via Histonet : > > Microm HM325 gets my vote. > > -----Original Message----- > From: Dawn Bugge via Histonet [histonet at lists.utsouthwestern.edu] > Received: Wednesday, 13 Sep 2017, 3:04PM > To: histonet at lists.utsouthwestern.edu [histonet at lists.utsouthwestern.edu] > Subject: [Histonet] What is the best microtome? (EXTERNAL EMAIL) > > Hello everyone, > > What is your favorite microtome? I am helping a lab with purchases and I > only have experience with the Finesse ME+ and an old Leica. Any thoughts > would be great. > > Thanks. > > -- > Dawn R Bugge > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, > is for the sole use of the intended recipient(s) and may contain confidential > or legally privileged information. Any unauthorized review, use, disclosure > or distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of this original message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jpiche at wtbyhosp.org Thu Sep 14 07:08:29 2017 From: jpiche at wtbyhosp.org (Piche, Jessica) Date: Thu, 14 Sep 2017 12:08:29 +0000 Subject: [Histonet] Xylene resistant gloves and xylene replacers Message-ID: <631955447A364B45B9458D29056351100116BCEF4D@WIN08-MBX-01.wtbyhosp.org> Good Morning Histonetters: I have two questions. Are there any gloves in existence (that are user friendly) that are resistant to xylene? And are there any xylene replacers out there that are actually safer than xylene? Thanks in advance and have a great day! Jessica Piche, HT (ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital From relia1 at earthlink.net Thu Sep 14 12:17:18 2017 From: relia1 at earthlink.net (relia1) Date: Thu, 14 Sep 2017 13:17:18 -0400 Subject: [Histonet] Hope to see you tomorrow at NSH SC in Orlando. Message-ID: Hi Histonetters!It has been quite a week here in Florida!I'm looking forward to getting back some normalcy and hugging all of my histopeeps at the NSH/SC.This is the time each year that I get to meet in person the people I work with all year long.If you are attending I hope to have the?opportunity to say hi.If you are looking for me I can usually be found at the registration desk.You can also reach me on my cell at 407-353-5070.Hope to see you soon.? Thanks-PamRight Place, Right Time, Right Move with RELIA! Thank you,?Pam BarkerPresident?RELIA Solutions(407)353-5070relia1 at earthlink.net? ?Sent from my Sprint Samsung Galaxy S7. From luigi at medequipsource.com Thu Sep 14 13:50:07 2017 From: luigi at medequipsource.com (Luigi Mascio) Date: Thu, 14 Sep 2017 14:50:07 -0400 Subject: [Histonet] What is the best microtome? In-Reply-To: References: Message-ID: <2C7597ABF5467247BE159D763E27F83901773FACCF65@PROMETHEUS.medequip.local> Dawn I can give you my opinion as a registered histologist, former Microm sales rep, and 15 years owner of Medical Equipment Source -where we re-manufacture instruments on a daily basis for histology. Please call me at 724-369-1604 Luigi M Mascio BS HT(ASCP) CEO Medical Equipment Source -----Original Message----- From: Dawn Bugge [mailto:drbugge at gmail.com] Sent: Wednesday, September 13, 2017 6:03 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] What is the best microtome? Hello everyone, What is your favorite microtome? I am helping a lab with purchases and I only have experience with the Finesse ME+ and an old Leica. Any thoughts would be great. Thanks. -- Dawn R Bugge From sbaldwin at mhhcc.org Thu Sep 14 14:51:42 2017 From: sbaldwin at mhhcc.org (Baldwin, Kathy) Date: Thu, 14 Sep 2017 19:51:42 +0000 Subject: [Histonet] Med tech Message-ID: <77ef4aea7bd940889a1a754058579e68@exch1.mhhcc.org> Hi fellow histonetters Was wondering if anyone can help me, my organization has a couple of permanent Med Tech positions open we are currently using temps but would love to see if anyone out there knows someone that would like a permanent job. I know this is for histologists but we all know a med tech or two :) S Kathy Baldwin ASCP, SCT Histology and Cytology Supervisor Ph 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From funderwood at mcohio.org Thu Sep 14 15:06:05 2017 From: funderwood at mcohio.org (Underwood, Fred) Date: Thu, 14 Sep 2017 20:06:05 +0000 Subject: [Histonet] Histostar embedding center Message-ID: <988516cb58a84a41803a8bb3bb7010ea@Exch2013-mb03.mcohio.org> Greetings Histonetters, Would someone be so kind as to provide me the dimensions of the cassette and base mold compartments for the Thermo Histostar embedding center? Thank you, Fred Underwood From jcox90 at yahoo.com Thu Sep 14 18:32:45 2017 From: jcox90 at yahoo.com (Jill Cox) Date: Thu, 14 Sep 2017 23:32:45 +0000 (UTC) Subject: [Histonet] Lab equipment insurance References: <1118224725.28483.1505431965718.ref@mail.yahoo.com> Message-ID: <1118224725.28483.1505431965718@mail.yahoo.com> Can anyone recommend a good insurance company/policy for your Histology lab equipment. Thank you!! Jill Cox HT ASCP From ronald.kusters at pfmmedical.com Fri Sep 15 03:04:07 2017 From: ronald.kusters at pfmmedical.com (Kusters, Ronald) Date: Fri, 15 Sep 2017 08:04:07 +0000 Subject: [Histonet] What is the best microtome? In-Reply-To: References: Message-ID: <7d8111d743ff4b85ab14166823ca9867@EX-PFM-103-W.pfm-ag.net> Dear Dawn, I'm less objective but you might look at this series. I will be at the NSH and can show it to you at the Cancer Diagnostics booth this weekend. And all Made in Germany, you can't say that from the mentioned brands! https://www.pfmmedical.com/en/productcatalogue/rotary_microtomes/index.html Yours, Ronald Mit freundlichen Gr??en/Kind Regards Ronald Kusters International Sales Manager Feather Brand Histotechnology pfm medical ag Wankelstra?e 60 50996 K?ln, Germany T +49 (2236) 9641 660 F +49 (2236) 9641 99 660 M +49 (171) 9144343 -----Original Message----- From: Dawn Bugge [mailto:drbugge at gmail.com] Sent: donderdag 14 september 2017 0:03 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] What is the best microtome? Hello everyone, What is your favorite microtome? I am helping a lab with purchases and I only have experience with the Finesse ME+ and an old Leica. Any thoughts would be great. Thanks. -- Dawn R Bugge From universalderm.nroberts at gmail.com Fri Sep 15 07:00:07 2017 From: universalderm.nroberts at gmail.com (Nicole Roberts) Date: Fri, 15 Sep 2017 08:00:07 -0400 Subject: [Histonet] Xylene resistant gloves and xylene replacers (Piche, Jessica) Message-ID: Good Morning, We are currently using XS-3 from StatLab and Formula 83 from CBG Biotech. Both of these are xylene substitutes and both are aliphatic hydrocarbons. They are less toxic than xylene, and don't have the strong smell we all learn to love about xylene. Limonene based substitutes are also an option but some techs have an issue with the smell. As for chemical resistance - Nitrile gloves are probably going to be your best bet for staining and general use, but a thicker glove made of polyvinyl (PVA) is more suited for pour over or immersion. Hope this helps! Nicole Roberts, BS, HTL(ASCP) Histotechnologist universalderm.nroberts at gmail.com (616) 419-3607 From sbaldwin at mhhcc.org Fri Sep 15 07:53:15 2017 From: sbaldwin at mhhcc.org (Baldwin, Kathy) Date: Fri, 15 Sep 2017 12:53:15 +0000 Subject: [Histonet] med tech Message-ID: <5533bd6d84da408a89e861f679ab8980@exch1.mhhcc.org> Hi fellow histonetters Was wondering if anyone can help me, my organization has a couple of permanent Med Tech positions open we are currently using temps but would love to see if anyone out there knows someone that would like a permanent job. I know this is for histologists but we all know a med tech or two :) We are located in Jasper Indiana 47546 Memorial Hospital and Health Care Center mhhcc.org to apply. Jasper is a city in, and the county seat[6] of Bainbridge Township, Dubois County, Indiana, United States, located along the Patoka River. The population was 15,038 at the 2010 census making it the 48th largest city in Indiana. On November 4, 2007, Dubois County returned to the Eastern Time Zone, after having moved to the Central Time Zone the previous year. The land uses are mainly agriculture. Jasper is a regional center in Southwestern Indiana, noted for its heavily German Catholic ancestral roots.[7] Jasper has often been called the "Wood Capital of the World", boasting a large number of furniture companies, including Kimball International and Masterbrand Cabinets. Jasper is also home to the Southern Indiana Education Center (SIEC), Jasper Engines & Transmissions (largest re-manufacturer in the market), and to a satellite campus of Vincennes University. The Indiana Baseball Hall of Fame, which honors players and others associated with the national pastime who were born or lived in Indiana, is located in Jasper. Please call with any questions. S Kathy Baldwin ASCP, SCT Histology and Cytology Supervisor Ph 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information or otherwise protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From jvickroy at SpringfieldClinic.com Fri Sep 15 10:46:48 2017 From: jvickroy at SpringfieldClinic.com (Vickroy, James) Date: Fri, 15 Sep 2017 15:46:48 +0000 Subject: [Histonet] CAP question ANP.12500 Message-ID: <9B1A1501A800064397369BD8072E6BCA6D51739B@E2K10DB.springfieldclinic.com> Wondered if something has changed on the question pertaining to retention. We only do biopsies and keep the containers they come in for two days. We have done this forever. Going through the new checklist I notice under wet tissue it has (stock bottle). Before I interpret this incorrectly, a stock bottle to me meant left over fixed tissue not taken for sections. Last week I got an email saying this other institution was keeping the empty containers for two weeks after final report. Obviously, it is clear if we have left over tissue we hold it for the specified time period but does this also mean we should be holding the empty biopsy container for two weeks plus also? I hope I am overthinking this since we don't have room for them. Your thoughts? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvickroy at SpringfieldClinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. From akemiat3377 at gmail.com Fri Sep 15 17:21:37 2017 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Fri, 15 Sep 2017 15:21:37 -0700 Subject: [Histonet] CAP question ANP.12500 In-Reply-To: <9B1A1501A800064397369BD8072E6BCA6D51739B@E2K10DB.springfieldclinic.com> References: <9B1A1501A800064397369BD8072E6BCA6D51739B@E2K10DB.springfieldclinic.com> Message-ID: We keep ours for one week after final report. There have been past issues as to tissues submitted from our endoscopy center not matching up to our gross exams. Gastric tissue included with esophageal tissue, I account for every MINUTE fragment, so there were absolutely floaters from somewhere!. We account for everything when I gross the tissue! I have a embedding log sheet which accounts for every piece submitted, as well as who embeds it, cuts, it, etc.. as well as any comments which just don?t match up to the gross. Akemi Allison BS, HT/HTL (ASCP) Pathology Manager Monterey Bay GI Consultants Laboratory 23 Upper Ragsdale Drive, Suite 200 Monterey, CA 93940 W: Email: aallison at montereygi.com H: Email: akemiat3377 at gmail.com Tele: (831) 375-3577 X117 Cell: (408) 335-9994 > On Sep 15, 2017, at 8:46 AM, Vickroy, James via Histonet wrote: > > Wondered if something has changed on the question pertaining to retention. We only do biopsies and keep the containers they come in for two days. We have done this forever. Going through the new checklist I notice under wet tissue it has (stock bottle). Before I interpret this incorrectly, a stock bottle to me meant left over fixed tissue not taken for sections. Last week I got an email saying this other institution was keeping the empty containers for two weeks after final report. > > Obviously, it is clear if we have left over tissue we hold it for the specified time period but does this also mean we should be holding the empty biopsy container for two weeks plus also? > > I hope I am overthinking this since we don't have room for them. > > Your thoughts? > > Jim > > Jim Vickroy > Histology Manager > Springfield Clinic, Main Campus, East Building > 1025 South 6th Street > Springfield, Illinois 62703 > Office: 217-528-7541, Ext. 15121 > Email: jvickroy at SpringfieldClinic.com > > > > This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera at msu.edu Tue Sep 19 10:26:35 2017 From: portera at msu.edu (Amy Porter) Date: Tue, 19 Sep 2017 11:26:35 -0400 Subject: [Histonet] University Core Support Facilities Message-ID: <002701d3315b$ae577af0$0b0670d0$@edu> To all: I have been asked to perform a cost analysis and comparison of the rates we charge for services to our researchers here on campus and outside the university - I am looking for labs that would be will to share this information for both in house and outside clients - a lab that does both would be ideal. I would conduct these conversations off list once started to prevent overload of the list and keep your information confidential if needed. Thanks in advance - Amy Amy S. Porter, HT (ASCP) Michigan State University Investigative HistoPathology Lab Research Support Facility portera at msu.edu From LRaff at uropartners.com Tue Sep 19 10:33:58 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Tue, 19 Sep 2017 15:33:58 +0000 Subject: [Histonet] Can any histonetters help out locating a microbiology tech? Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF11621816@COLOEXCH01.uropartners.local> Hello all: We are looking for a part-time, mostly weekend, micro tech in the Chicago area. They are tough to find! If you know of anyone, or have any connections to micro in your lab, please pass this along. Thanks, Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From Timothy.Morken at ucsf.edu Tue Sep 19 11:33:55 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 19 Sep 2017 16:33:55 +0000 Subject: [Histonet] Replacement filters for Lerner Fume-Gard benchtop hood? Message-ID: Hi, does anyone know a source for the charcoal filters for the old Lerner Laboratories Fume-gard hood, model 912, filter #906? Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center From mgiorgi at incdx.com Tue Sep 19 12:25:54 2017 From: mgiorgi at incdx.com (Miranda Giorgi) Date: Tue, 19 Sep 2017 17:25:54 +0000 Subject: [Histonet] Running ISH on the Ventana Ultra Stainers Message-ID: Hello Histonetters, Our lab is in the process of trying to validate Kappa, Lambda and EBER ISH on the Ventana Ultra stainers. We have run these probes on the XT in the past with good results, but unfortunately staining on the Ultras is either poor or non-existent. We have tried several protocol modifications with very little luck. Does anybody out there have experience validating these tests on the Ultras? We would greatly appreciate any advice on getting these to work. Thanks in advance! This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700. From preyamenna at hotmail.com Tue Sep 19 23:37:42 2017 From: preyamenna at hotmail.com (Preya Menna) Date: Wed, 20 Sep 2017 04:37:42 +0000 Subject: [Histonet] Dako Artisan Elastic Van Gieson Stain. Lot reagent: 10125102 Message-ID: Dear all, In Histology, we are experiencing staining issues with the Dako Artisan Elastic Van Gieson Stain. Lot reagent: 10125102 The stained slides are patchy, uneven, with an excess of black/blue. Tissue used: Liver fixed in 10% neutral buffered formalin. Does anyone else have issues with this batch of reagents? Thanks From Jcox90 at yahoo.com Wed Sep 20 12:41:35 2017 From: Jcox90 at yahoo.com (Jill Cox) Date: Wed, 20 Sep 2017 10:41:35 -0700 Subject: [Histonet] Histology lab equipment relocation Message-ID: <44C88A50-AA8E-4B3E-B9EB-BE4566782271@yahoo.com> Hi all, Our lab is possibly moving to a different part of town and we are looking for someone experienced in moving lab equipment. Processor, strainer, embedding station, microtome, Ventana XT, miscellaneous other. Has anyone used a company or can you recommend someone? Thank you in advance! Sent from my iPhone From edmartin26 at gmail.com Wed Sep 20 13:20:43 2017 From: edmartin26 at gmail.com (Eddie Martin) Date: Wed, 20 Sep 2017 14:20:43 -0400 Subject: [Histonet] Running ISH on the Ventana Ultra Stainers Message-ID: <490CAFDB-1354-486D-A3A5-A96CD3F4BB57@gmail.com> Hi Miranda, For starters, perform a Decontamination on your Ultras, don't dilute your probes. At our lab, we manually dispense the probes but a prep kit is fine. And also use a toothpick to clear out any gunk in your detection dispensers prior to doing a run. Also, check if your ultras are on battery backups providing sufficient run time amperage in case of power failures or abnormalities. Also moving your ultras or the instrument not being level can create issues. And check that you lo of charged slides are staining well across several antibodies. If all that is ok, then try out your protocol again and see if you get adequate staining quality. Hope this helps to get you started! Eddie Martin, HT,HTL,QIHC IHC Histotechnologist III The Joint Pathology Center Walter Reed National Military Medical Center Bethesda, MD Sent from my iPhone > On Sep 19, 2017, at 1:25 PM, Miranda Giorgi wrote: > > Hello Histonetters, > > Our lab is in the process of trying to validate Kappa, Lambda and EBER ISH on the Ventana Ultra stainers. We have run these probes on the XT in the past with good results, but unfortunately staining on the Ultras is either poor or non-existent. We have tried several protocol modifications with very little luck. Does anybody out there have experience validating these tests on the Ultras? We would greatly appreciate any advice on getting these to work. > > Thanks in advance! > > > This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at privacy at incdx.com or call (509) 892-2700. > From greg.dobbin at gmail.com Thu Sep 21 08:38:26 2017 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Thu, 21 Sep 2017 10:38:26 -0300 Subject: [Histonet] Storage of blanks (Charged slides) Message-ID: Hi Folks, Can some of you share what your practices are regarding storage of blanks for possible subsequent IHC orders? Background: We cut a blank on every prostate core in case a PIN4 is ordered after the H&Es have been read. So each week, we have 72-100 blank slides to store. Space is an issue especially if we keep them in a freezer. Due to space constraints, we are currently storing these blank slides in slide filing fashion (ie front-to-back) in the -20 freezer. Questions: 1) How long are you keeping your blank slides? 2) Under what storage conditions are you keeping then (ie Temp, in a box, wrapped, stacked, etc)? Thank you, Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* From richard.wild at wanadoo.fr Thu Sep 21 23:41:51 2017 From: richard.wild at wanadoo.fr (richard wild) Date: Fri, 22 Sep 2017 06:41:51 +0200 Subject: [Histonet] autostainer slide stainer IHC repair manual Message-ID: <973984e3-8cd3-647a-fcbe-402945495c67@wanadoo.fr> Hi dear Histoneters, I am looking for a copy of autostainer slide stainer IHC repair manual. (my request is about where could be a sensor in the arm of the Y position (back to front) ?) Thanks R W From Teresa.Lima at bmc.org Fri Sep 22 10:47:37 2017 From: Teresa.Lima at bmc.org (Lima, Teresa) Date: Fri, 22 Sep 2017 15:47:37 +0000 Subject: [Histonet] Fisher SlideMate printers Message-ID: <1E9091127DC2184183D2D9BF2D7A6F0C77092F4F@ex-mb01.bmc.bmcroot.bmc.org> Has anyone had any issues with Fisher SlideMate slide printers and PrintMate cassettes printers? It seems like we are replacing print heads on the cassette printers far too often and the slide printers after only four weeks of use have already been replaced. I would be interested to hear if anyone else has experienced these problems. Thanks, Terri Terri Lima Histology Supervisor Department of Pathology Boston Medical Center 670 Albany Street Boston, MA 02118 617-414-5332 teresa.lima at bmc.org ---------------------------------------------------------------------- This electronic transmission may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, please notify me immediately as use of this information is strictly prohibited. From jramos at biocare.net Fri Sep 22 17:16:10 2017 From: jramos at biocare.net (Jason Ramos) Date: Fri, 22 Sep 2017 22:16:10 +0000 Subject: [Histonet] Technical Support Specialist Position Message-ID: <6634328c911940969f8e8d753aae827f@mail03.biocare-domain.com> Hello Histonet- Biocare Medical is searching for a Technical Support Specialist/Senior Technical Support Specialist to join our team! You will provide Technical/Applications support to customers and employees on all product-related issues via telephone, web, and email communication. The Technical Support Specialist will have general knowledge of reagents (IHC, Molecular, Detection, and Ancillaries) and Instrumentation (Small and large instrumentation, software). Responsibilities: * Answer incoming instrument and reagent calls from the Technical Support phone line; Answer Technical Support emails * Perform "phone fixes" for instrument issues for troubleshooting and training; enter all calls and emails in CRM * Ensure that all client calls are directed via Technical Support for initial triage followed by detailed analysis to determine if an FAS or FSE is required on-site * Provide technical guidance as needed to all internal Biocare departments * Track customer issues and resolutions Qualifications: * Knowledge of General Histology, IHC, ISH, and FISH techniques * Ability to build rapport with clients * Strong troubleshooting and critical thinking skills * Positive and professional demeanor * Ability to work in a high-pressured deadline-oriented environment If interested, please send your resume. Thank you, Jason Jason A. Ramos, Ph.D. | Biocare Medical Director of Education & Technical Support | o: 925.603.8098 | c: 925.768.2792 jramos at biocare.net From litepath2000 at yahoo.com Fri Sep 22 18:17:56 2017 From: litepath2000 at yahoo.com (LitePath) Date: Fri, 22 Sep 2017 23:17:56 +0000 (UTC) Subject: [Histonet] University Core Support Facilities In-Reply-To: <002701d3315b$ae577af0$0b0670d0$@edu> References: <002701d3315b$ae577af0$0b0670d0$@edu> Message-ID: <994798706.8069382.1506122276777@mail.yahoo.com> Hi AmyWould definitely like to chat, let me know when you might have some time and we can arrange a call? Luis ChiribogaNYULMC Sent from Yahoo Mail on Android On Tue, Sep 19, 2017 at 11:42 AM, Amy Porter via Histonet wrote: To all:? I have been asked to perform a cost analysis and comparison of the rates we charge for services to our researchers here on campus and outside the university - I am looking for labs that would be will to share this information for both in house and outside clients - a lab that does both would be ideal.? I would conduct these conversations off list once started to prevent overload of the list and keep your information confidential if needed.? Thanks in advance - Amy Amy S. Porter, HT (ASCP) Michigan State University Investigative HistoPathology Lab Research Support Facility portera at msu.edu _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From garethdavisyuma at gmail.com Mon Sep 25 14:56:03 2017 From: garethdavisyuma at gmail.com (Gareth Davis) Date: Mon, 25 Sep 2017 12:56:03 -0700 Subject: [Histonet] Backup Power Supply Message-ID: Hello All, ?We need back-up power supply via CAP regulations. Besides a generator or a dedicated supply through a hospital back-up generator, what are labs using. We are a small lab located in a clinic. There is no back-up power supply in the clinic. Wondered if there was anything out there that could be used, like a battery back-up. My only concern is our processor, which is run overnight. But, we need something. Thanks, -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 From paula at excaliburpathology.com Mon Sep 25 15:25:17 2017 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Mon, 25 Sep 2017 20:25:17 +0000 (UTC) Subject: [Histonet] Backup Power Supply In-Reply-To: References: Message-ID: <146910350.9708126.1506371117994@mail.yahoo.com> Hi,? this is one that can charge by being plugged in and is larger than ones for computers. http://www.wisefoodstorage.com/humless-portable-power-storage-and-fuelless-generator.html They also have larger ones. I have no relationship with Wise, except being a customer.?Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com From: Gareth Davis via Histonet To: "Histonet at lists.utsouthwestern.edu" Sent: Monday, September 25, 2017 3:18 PM Subject: [Histonet] Backup Power Supply Hello All, ?We need back-up power supply via CAP regulations.? Besides a generator or a dedicated supply through a hospital back-up generator, what are labs using.? We are a small lab located in a clinic.? There is no back-up power supply in the clinic.? Wondered if there was anything out there that could be used, like a battery back-up.? My only concern is our processor, which is run overnight.? But, we need something. Thanks, -- *Ms. Gareth B. Davis*, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ASelf at tidelandshealth.org Tue Sep 26 08:31:25 2017 From: ASelf at tidelandshealth.org (Amy Self) Date: Tue, 26 Sep 2017 09:31:25 -0400 Subject: [Histonet] Amputated Limb Storage Message-ID: Good morning, How is everyone storing their amputated limbs that are received in pathology? Are they stored in the morgue or in a refrigerator located within the department and at what temperature is suggested for the storage of amputated limbs? Thanks in advance, Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From rsrichmond at gmail.com Tue Sep 26 15:33:18 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Tue, 26 Sep 2017 16:33:18 -0400 Subject: [Histonet] Amputated Limb Storage Message-ID: Amy Self, Histology Lab Senior Tech, Tidelands Georgetown Memorial Hospital, Georgetown SC asks: >>How is everyone storing their amputated limbs that are received in pathology? Are they stored in the morgue or in a refrigerator located within the department and at what temperature is suggested for the storage of amputated limbs?<< Here's the opinion of an elderly pathologist who's dissected his share of these (personal record is six in a day). Do NOT let these specimens sit around, because they stink, some of them when they arrive, and they can easily ruin a refrigerator with an ineradicable smell. Your pathologist must dissect them promptly, and they should be stored in the morgue, and disposed of as fast as possible. I never heard of anybody going back to the gross specimen, at least I never have. I've walked a good many of them to the hospital incinerator (way back when) myself. Bob Richmond Samurai Pathologist Maryville TN From Christopher.Hagon at act.gov.au Tue Sep 26 17:29:21 2017 From: Christopher.Hagon at act.gov.au (Hagon, Christopher (Health)) Date: Tue, 26 Sep 2017 22:29:21 +0000 Subject: [Histonet] Fisher SlideMate printers [SEC=UNCLASSIFIED] Message-ID: Apparently the printheads don't like brands of cassettes made of harder plastic, the pins tend to pop out resulting in poor printing. I believe there is a list of 'approved cassettes' in the back of the manual. Or call your rep, they should be able to point you in the right direction. I think the expected life of the printheads is about 50,000 cassettes. Hope that helps, Chris Hagon Senior Scientist, Anatomical Pathology ACT Pathology Level 3, Building 10 The Canberra Hospital -----Original Message----- From: Lima, Teresa [mailto:Teresa.Lima at bmc.org] Sent: Saturday, 23 September 2017 1:48 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fisher SlideMate printers Has anyone had any issues with Fisher SlideMate slide printers and PrintMate cassettes printers? It seems like we are replacing print heads on the cassette printers far too often and the slide printers after only four weeks of use have already been replaced. I would be interested to hear if anyone else has experienced these problems. Thanks, Terri Terri Lima Histology Supervisor Department of Pathology Boston Medical Center 670 Albany Street Boston, MA 02118 617-414-5332 teresa.lima at bmc.org ---------------------------------------------------------------------- This electronic transmission may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the intended recipient, please notify me immediately as use of this information is strictly prohibited. ----------------------------------------------------------------------- This email, and any attachments, may be confidential and also privileged. If you are not the intended recipient, please notify the sender and delete all copies of this transmission along with any attachments immediately. You should not copy or use it for any purpose, nor disclose its contents to any other person. ----------------------------------------------------------------------- From calhouan at ohsu.edu Wed Sep 27 14:26:16 2017 From: calhouan at ohsu.edu (Andrea Calhoun) Date: Wed, 27 Sep 2017 19:26:16 +0000 Subject: [Histonet] Mammary Tissue Processing & Sectioning Troubles Message-ID: <5030A8B38F932048A3DECBBA52ECB05C012BBB4F85@EXMB12.ohsu.edu> Hi All! I'm working with primate mammary tissue that was given to us from a pathology group off campus. They say the tissue was fixed in NBF for at least two days, and sent to us in 70% EtOH, where it has sat for a couple months. We are treating these samples as we would with human tissue. After grossing, processing (14hr schedule), and sectioning (4-5um, high-profile blade)), I find the tissue is difficult/ near impossible to section. Even after leaving the blocks on ice for 30+min, the tissue continuously mushes against the blade. If I do get it to cut, multiple knife marks develop quickly and I find I am going through blades like crazy. I am staying superficial incase the fix didn't penetrate deep into the tissue. So far I've only processed a few samples from a larger group to resolve these issues before processing the remainder. Does anyone have any tips for processing or sectioning? Besides grossing the tissue into smaller pieces, would it help to re-fix them overnight or an additional day? How would you tell if the issue is from fixation or insufficient clearing/infiltration of paraffin? Any information would be appreciated! Andrea Calhoun B.S., CEMT Research Assistant 2, Schedin Lab Dept. of Cell, Developmental, & Cancer Biology Oregon Health & Sciences University calhouan at ohsu.edu , RJH-5350 From saby_joseph_a at yahoo.com Wed Sep 27 15:22:49 2017 From: saby_joseph_a at yahoo.com (Joseph Saby) Date: Wed, 27 Sep 2017 20:22:49 +0000 (UTC) Subject: [Histonet] Mammary Tissue Processing & Sectioning Troubles In-Reply-To: <5030A8B38F932048A3DECBBA52ECB05C012BBB4F85@EXMB12.ohsu.edu> References: <5030A8B38F932048A3DECBBA52ECB05C012BBB4F85@EXMB12.ohsu.edu> Message-ID: <969629635.10593874.1506543769498@mail.yahoo.com> Andrea- I believe your tissue was not fully fixed.? Many labs think they know more than they do, so itis always a good idea to pin down exactly what they did to fix the tissue.? I am guessing they left thick blobs of tissue in an inadequate volume of formalin and expected that to do the trick. I would deparaffinize and rehydrate the tissues, then place them back in an appropriate volume of formalin and fix them until you know they will be fixed.? If the sections are thick, cut them thinner for this fixation.? After reprossessing, these tissues should section much better. Good luck! Joe Saby, retired ? From: Andrea Calhoun via Histonet To: "histonet at lists.utsouthwestern.edu" Sent: Wednesday, September 27, 2017 3:49 PM Subject: [Histonet] Mammary Tissue Processing & Sectioning Troubles Hi All! I'm working with primate mammary tissue that was given to us from a? pathology group off campus. They say the tissue was fixed in NBF for at least two days, and sent to us in 70% EtOH, where it has sat for a couple months.? We are treating these samples as we would with human tissue.? After grossing, processing (14hr schedule), and sectioning (4-5um, high-profile blade)), I find the tissue is difficult/ near impossible to section.? Even after leaving the blocks on ice for 30+min, the tissue continuously mushes against the blade.? If I do get it to cut, multiple knife marks develop quickly and I find I am going through blades like crazy.? I am staying superficial incase the fix didn't penetrate? deep into the tissue.? ? So far I've only processed a few samples from a larger group to resolve these issues before processing the remainder. Does anyone have any tips for processing or sectioning?? Besides grossing the tissue into smaller pieces, would it help to re-fix them overnight or an additional day? How would you tell if the issue is from fixation or insufficient clearing/infiltration of paraffin? Any information would be appreciated! Andrea Calhoun B.S., CEMT Research Assistant 2, Schedin Lab Dept. of Cell, Developmental, & Cancer Biology Oregon Health & Sciences University calhouan at ohsu.edu , RJH-5350 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Shannon.Logan at bellin.org Wed Sep 27 15:31:51 2017 From: Shannon.Logan at bellin.org (Logan, Shannon) Date: Wed, 27 Sep 2017 20:31:51 +0000 Subject: [Histonet] Mammary Tissue Processing & Sectioning Troubles In-Reply-To: <5030A8B38F932048A3DECBBA52ECB05C012BBB4F85@EXMB12.ohsu.edu> References: <5030A8B38F932048A3DECBBA52ECB05C012BBB4F85@EXMB12.ohsu.edu> Message-ID: <969c600d81754f9390f62cbc9770c21f@BAPWEXCH001a.bellin.com> Hi Andrea, Tissue that is not fixed properly will be mushy and difficult to section, just as you described. Was there a reason NOT to send it in NBF for storage? I think the main problem is with the storage in 70%. Or there is a small possibility the 14 hr schedule did not remove all of the water from the tissue. However, I tend to doubt the tissue was fixed in the first place. If they said the tissue was fixed for 2 days in NBF I would question that as well. If it was never fixed or not fixed long enough, then storage in 70% would be detrimental to proper fixation in the 14 hr schedule. Shannon H. Logan BS, HTL (ASCP) Bellin Health Pathology Dept. 744 South Webster Ave. Green Bay, WI 54305 920-433-3653 From: Andrea Calhoun via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, September 27, 2017 2:26 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Mammary Tissue Processing & Sectioning Troubles Hi All! I'm working with primate mammary tissue that was given to us from a pathology group off campus. They say the tissue was fixed in NBF for at least two days, and sent to us in 70% EtOH, where it has sat for a couple months. We are treating these samples as we would with human tissue. After grossing, processing (14hr schedule), and sectioning (4-5um, high-profile blade)), I find the tissue is difficult/ near impossible to section. Even after leaving the blocks on ice for 30+min, the tissue continuously mushes against the blade. If I do get it to cut, multiple knife marks develop quickly and I find I am going through blades like crazy. I am staying superficial incase the fix didn't penetrate deep into the tissue. So far I've only processed a few samples from a larger group to resolve these issues before processing the remainder. Does anyone have any tips for processing or sectioning? Besides grossing the tissue into smaller pieces, would it help to re-fix them overnight or an additional day? How would you tell if the issue is from fixation or insufficient clearing/infiltration of paraffin? Any information would be appreciated! Andrea Calhoun B.S., CEMT Research Assistant 2, Schedin Lab Dept. of Cell, Developmental, & Cancer Biology Oregon Health & Sciences University calhouan at ohsu.edu , RJH-5350 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kkraus0344 at gmail.com Thu Sep 28 12:05:47 2017 From: kkraus0344 at gmail.com (Kevin J Kraus) Date: Thu, 28 Sep 2017 17:05:47 +0000 Subject: [Histonet] S Message-ID: He From Richard.Cartun at hhchealth.org Thu Sep 28 15:19:45 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Thu, 28 Sep 2017 20:19:45 +0000 Subject: [Histonet] Mass Spectrometry Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E9546C07E@HHCEXCHMB03.hhcsystem.org> Anyone working in Anatomic Pathology and using mass spectrometry for protein analysis on cancer cells? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From jford at cytomx.com Thu Sep 28 17:14:16 2017 From: jford at cytomx.com (Judi Ford) Date: Thu, 28 Sep 2017 22:14:16 +0000 Subject: [Histonet] Translational science/medicine/research IHC lab question Message-ID: Hi Everyone, I am interested in connecting with techs/managers who are working in or have developed a translation science/medicine/research IHC lab. Recently my department has become a translational science department and I run most of the IHC. I'm interested in building this lab into a good translational ihc lab and was wondering about the following: 1. What does being a translational ihc lab mean? 2. What advice would you have in building a translational ihc lab? 3. What equipment would you want in a translational ihc lab that isn't necessarily in a regular research ihc lab? 4. Are you available offline for further questions? I really appreciate any guidance you send my way. I'm looking at this as a long term goal with the possibility of becoming the manager of the lab. Thanks sooooo much in advance for any advice. Hope you all have a really great weekend :) Best regards, Judi Gordon SRA Translational Sciences CytomX Therapeutics South San Francisco, CA STATEMENT OF CONFIDENTIALITY: The information contained in this electronic message and any attachments to this message are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not an intended recipient, or a person responsible for delivering the e-mail to an intended recipient, please be advised that you have received this message in error and that any use, dissemination, forwarding, printing, or copying is strictly prohibited. Please notify the sender at CytomX Therapeutics, Inc., immediately and destroy all copies of this message and any attachments. CytomX Therapeutics, Inc. From cforster at umn.edu Thu Sep 28 17:18:52 2017 From: cforster at umn.edu (Colleen Forster) Date: Thu, 28 Sep 2017 17:18:52 -0500 Subject: [Histonet] Translational science/medicine/research IHC lab question In-Reply-To: References: Message-ID: I am following, would love to see the responses. Colleen Forster On Thu, Sep 28, 2017 at 5:14 PM, Judi Ford via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Everyone, > I am interested in connecting with techs/managers who are working in or > have developed a translation science/medicine/research IHC lab. Recently my > department has become a translational science department and I run most of > the IHC. I'm interested in building this lab into a good translational ihc > lab and was wondering about the following: > > 1. What does being a translational ihc lab mean? > > 2. What advice would you have in building a translational ihc lab? > > 3. What equipment would you want in a translational ihc lab that > isn't necessarily in a regular research ihc lab? > > 4. Are you available offline for further questions? > I really appreciate any guidance you send my way. I'm looking at this as a > long term goal with the possibility of becoming the manager of the lab. > Thanks sooooo much in advance for any advice. Hope you all have a really > great weekend :) > Best regards, > Judi Gordon > SRA > Translational Sciences > CytomX Therapeutics > South San Francisco, CA > > > STATEMENT OF CONFIDENTIALITY: > > The information contained in this electronic message and any attachments > to this message are intended for the exclusive use of the addressee(s) and > may contain confidential or privileged information. If you are not an > intended recipient, or a person responsible for delivering the e-mail to an > intended recipient, please be advised that you have received this message > in error and that any use, dissemination, forwarding, printing, or copying > is strictly prohibited. Please notify the sender at CytomX Therapeutics, > Inc., immediately and destroy all copies of this message and any > attachments. > > CytomX Therapeutics, Inc. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kmilne at bccrc.ca Fri Sep 29 12:21:09 2017 From: kmilne at bccrc.ca (Katy Milne) Date: Fri, 29 Sep 2017 17:21:09 +0000 Subject: [Histonet] Translational science/medicine/research IHC lab question In-Reply-To: References: Message-ID: I run the histology lab at the Deeley Research Centre in Victoria BC, we're a combination of basic research (mostly mouse projects) and translational research (often samples from clinical trials and other human-based research projects). Translational research is just really the bridge between bench and bedside so you're looking mostly at human samples where the outcome of the research could translate into downstream benefits to human health. It's hard to say what advice I would have for setting up a translational lab but you can certainly hit me up offline with any questions you may have and I can help where I can. As for equipment you'd basically have what you have in a basic IHC lab. Beyond that it would depend on what sort of services you want to offer. We do a lot of multicolour IHC so we have a multispectral imaging system (Vectra) and we have automated scoring software (Inform) as a lot of our clients don't have an in-house pathologist available to them so they just want an excel file of counts at the end of the day. We also do multicolour IF so we use the same system for that. We've also been working on developing the molecular end of things with ISH and in situ PCR, possibly Nanostring down the road but typically these have been backburnered as we've been so busy with the multicolour IHC projects. Good luck! Katy Milne Molecular and Cellular Immunology Core Trev and Joyce Deeley Research Centre Message: 3 Date: Thu, 28 Sep 2017 22:14:16 +0000 From: Judi Ford To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Translational science/medicine/research IHC lab question Message-ID: Content-Type: text/plain; charset=WINDOWS-1252 Hi Everyone, I am interested in connecting with techs/managers who are working in or have developed a translation science/medicine/research IHC lab. Recently my department has become a translational science department and I run most of the IHC. I'm interested in building this lab into a good translational ihc lab and was wondering about the following: 1. What does being a translational ihc lab mean? 2. What advice would you have in building a translational ihc lab? 3. What equipment would you want in a translational ihc lab that isn't necessarily in a regular research ihc lab? 4. Are you available offline for further questions? I really appreciate any guidance you send my way. I'm looking at this as a long term goal with the possibility of becoming the manager of the lab. Thanks sooooo much in advance for any advice. Hope you all have a really great weekend :) Best regards, Judi Gordon SRA Translational Sciences CytomX Therapeutics South San Francisco, CA