From relia1 at earthlink.net Tue Oct 3 10:27:03 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 3 Oct 2017 11:27:03 -0400 Subject: [Histonet] The NSH/SC was GREAT!! 2017 was GREAT!! And My phone is still ringing with great opportunities in FL, NC, VA, CA, MA and more!!! Message-ID: <006501d33c5c$117d52c0$3477f840$@earthlink.net> Hello Histonetters! How are you doing? I had such a great time at the NSHSC right here in my hometown ? Orlando, FL!!! I enjoyed making new friends, reconnecting with old friends and learning new things. It was touch and go for awhile there with Hurricane Irma?s unwelcome visit but we are #orlandostrong and #histostrong We gave blood to support the victims of Hurricane Irma and the bloodmobile ran out of bags!! There was an amazing and inspiring speaker provided by Roche! The hotel was absolutely gorgeous! AND So much to see and do here in Orlando!!!! But the most fun of all I worked the registration desk with some really fun histotechs! I posted some pictures on Facebook, Twitter and Instagram with the Hashtag #NSHSC. If you get a chance check them out!! I really encourage everyone to go to meetings when they can. If you can?t make the national meeting try to make it to a regional or state meeting. The camaraderie and educational opportunities are phenomenal. Next year the convention will be held in St. Louis, MO Hope to see you there!!! ? My phone never stopped ringing while I was gone!! ? All of my positions are full time permanent positions. ? My clients offer excellent compensation, benefits and relocation assistance. **And they are ready to interview and hire right away!** I Have Management Opportunities in: North Carolina California Florida Alabama Virginia I have HT/HTL and eligible positions in: Florida Alabama North Carolina Massachusetts Tennessee Of course I can?t put all the information about these opportunities in an e-mail. So if you or anyone you know might be interested in hearing more about any of these positions or want help with a job search in another area please contact me. I can be reached at 866-607-3542 or on my cell at 407-353-5070 or relia1 at earthlink.net. Remember it never hurts to look. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From esolanki at pathmdlabs.com Tue Oct 3 11:44:03 2017 From: esolanki at pathmdlabs.com (Evan Solanki) Date: Tue, 3 Oct 2017 16:44:03 +0000 Subject: [Histonet] PTEN Question Message-ID: Hello, I need assistance with PTEN validation. I am looking for information on positive PTEN control slides. Is there any company which can supply these control slides? Are there any indicators that we might be able to use (Positive PIN4, Positive ERG) that might lead us to determine beforehand which tissue will show loss of PTEN expression? This is what we are looking for in our validation. Any help would be greatly appreciated! From julio.benavides.silvan at csic.es Tue Oct 3 12:12:59 2017 From: julio.benavides.silvan at csic.es (Julio Benavides) Date: Tue, 3 Oct 2017 19:12:59 +0200 Subject: [Histonet] Best second chromogen with DAB IHC? In-Reply-To: References: Message-ID: <410d9a01-0bd9-94b8-4421-e3a31f3fa2a0@csic.es> Hi there, we want to do some double labelling, one of the chromogens being DAB and haematoxylin the counter stain. Which chromogen would you choose for the second labelling? We use envision-HRP as secondary As always, thanks a lot for your help! Cheers julio Julio Benavides Silv?n Instituto de Ganader?a de Monta?a (CSIC-ULE) Grulleros, Le?n +34 987317156 From hans at histologistics.com Tue Oct 3 12:41:36 2017 From: hans at histologistics.com (Hans B Snyder) Date: Tue, 3 Oct 2017 13:41:36 -0400 Subject: [Histonet] PTEN Question (Evan Solanki) Message-ID: Hello, I am by no expert in PTEN expression but we do a fair amount of antibody workups. For new antibodies that we don't have known protocols and or types of tissues we refer to "the human protein atlas". This gives volumes of information like both normal and abnormal tissue expression, and many other facts that may be relevant. Here is the link specifically to PTEN https://www.proteinatlas.org/ENSG00000171862-PTEN/tissue Good luck! Hans B Snyder JMD Histology & Histologistics Inc. 151 W Main Street Dudley, MA 01571 Lab - 508-461-7207 C- 508-308-7800 www.histologistics.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. On Tue, Oct 3, 2017 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. The NSH/SC was GREAT!! 2017 was GREAT!! And My phone is still > ringing with great opportunities in FL, NC, VA, CA, MA and > more!!! (Pam Barker) > 2. PTEN Question (Evan Solanki) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 3 Oct 2017 11:27:03 -0400 > From: "Pam Barker" > To: "Histonetters Histonet" > Subject: [Histonet] The NSH/SC was GREAT!! 2017 was GREAT!! And My > phone is still ringing with great opportunities in FL, NC, > VA, CA, MA > and more!!! > Message-ID: <006501d33c5c$117d52c0$3477f840$@earthlink.net> > Content-Type: text/plain; charset="iso-8859-1" > > Hello Histonetters! > How are you doing? I had such a great time at the NSHSC right here in my > hometown ? Orlando, FL!!! > I enjoyed making new friends, reconnecting with old friends and learning > new > things. > It was touch and go for awhile there with Hurricane Irma?s unwelcome visit > but we are #orlandostrong and #histostrong > > We gave blood to support the victims of Hurricane Irma and the bloodmobile > ran out of bags!! > There was an amazing and inspiring speaker provided by Roche! > The hotel was absolutely gorgeous! > AND So much to see and do here in Orlando!!!! > > But the most fun of all? > I worked the registration desk with some really fun histotechs! > I posted some pictures on Facebook, Twitter and Instagram with the > Hashtag #NSHSC. > If you get a chance check them out!! > I really encourage everyone to go to meetings when they can. > If you can?t make the national meeting try to make it to a regional or > state > meeting. The camaraderie and educational opportunities are phenomenal. > > Next year the convention will be held in St. Louis, MO Hope to see you > there!!! > > ? My phone never stopped ringing while I was gone!! > ? All of my positions are full time permanent positions. > ? My clients offer excellent compensation, benefits and relocation > assistance. > **And they are ready to interview and hire right away!** > > I Have Management Opportunities in: > North Carolina > California > Florida > Alabama > Virginia > > I have HT/HTL and eligible positions in: > Florida > Alabama > North Carolina > Massachusetts > Tennessee > > Of course I can?t put all the information about these opportunities in an > e-mail. So if you or anyone you know might be interested in hearing more > about any of these positions or want help with a job search in another area > please contact me. I can be reached at 866-607-3542 or on my cell at > 407-353-5070 or relia1 at earthlink.net. > > Remember it never hurts to look. > Thanks-Pam > > Right Place, Right Time, Right Move with RELIA! > > Thank You! > ?Pam M. Barker > ? > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5703 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell:???? (407)353-5070 > FAX:???? (407)678-2788 > E-mail: relia1 at earthlink.net > www.facebook.com/PamBarkerRELIA > www.linkedin.com/in/reliasolutions > www.twitter.com/pamatrelia > > > > > > ------------------------------ > > Message: 2 > Date: Tue, 3 Oct 2017 16:44:03 +0000 > From: Evan Solanki > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] PTEN Question > Message-ID: > pathmdlabs.local> > Content-Type: text/plain; charset="us-ascii" > > Hello, > > I need assistance with PTEN validation. I am looking for information on > positive PTEN control slides. > Is there any company which can supply these control slides? > Are there any indicators that we might be able to use (Positive PIN4, > Positive ERG) that might lead us to determine beforehand which tissue will > show loss of PTEN expression? This is what we are looking for in our > validation. > > Any help would be greatly appreciated! > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 167, Issue 1 > **************************************** > From MLashus at pathgroup.com Tue Oct 3 14:12:50 2017 From: MLashus at pathgroup.com (Mighnon Lashus) Date: Tue, 3 Oct 2017 19:12:50 +0000 Subject: [Histonet] Sakura 601 Stainer Manual Message-ID: Would anyone happen to have a manual for the Sakura 601 stainer on PDF they would be willing to share? Thanks, Mighnon Lashus, HT (ASCP) Laboratory Manager PathGroup 4071 S. Access Rd, Suite 107 Chattanooga, TN 37406 423-493-0207 423-493-0208 fax mlashus at pathgroup.com Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup immediately at 615-562-9255. Thank you From bernardgregoryamparo at gmail.com Tue Oct 3 23:27:07 2017 From: bernardgregoryamparo at gmail.com (Bernard Gregory Amparo) Date: Wed, 4 Oct 2017 07:27:07 +0300 Subject: [Histonet] Experience for doing of frozen section for bones Message-ID: Good day, I would like to know if you have any protocols or procedures to share regarding the frozen sectioning of bones? Thank you in advance From relia1 at earthlink.net Wed Oct 4 09:54:04 2017 From: relia1 at earthlink.net (Pam Barker) Date: Wed, 4 Oct 2017 10:54:04 -0400 Subject: [Histonet] RELIA Special Job Alert for Managers and Supervisors 10-4-2017 Exciting opportunities Nationwide NC*FL*CA*VA*MA*AL* Message-ID: <10dd01d33d20$9f6858e0$de390aa0$@earthlink.net> Hello Histonetters, I have several exciting opportunities for experienced Managers and Supervisors in hospital and private lab environments in several locations throughout the US. These are some of the premier employers in the United States. The positions are of course full time and permanent. My clients offer excellent compensation, benefits and relocation assistance. Here are my leadership positions: AP Manager - Charlotte, NC Mohs Histology Supervisor - Naples, FL Histology Supervisor - Salem, VA Histology Supervisor - Modesto, CA Histology Supervisor - Boston, MA Histology Supervisor - Birmingham, AL I anticipate multiple management openings with many exciting opportunities for talented managers and supervisor to be coming available nationwide as many of my clients are experiencing rapid growth again. If you are interested in looking into making a job change please let me know. That way I can keep you apprised of management opportunities that would interest you. Also if you would like more information or know of someone else who might be interested, please contact me via email at: relia1 at earthlink.net or toll free at: 866-607-3542 or on my cell at: 407-353-5070. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam There are a lot of recruiters out there right now trying to work with histology professionals and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 15 years I have dedicated my practice solely to placing histology professionals like you. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From Katie at PuyallupDerm.com Wed Oct 4 12:47:59 2017 From: Katie at PuyallupDerm.com (Katie) Date: Wed, 4 Oct 2017 10:47:59 -0700 Subject: [Histonet] Substitute Mohs Tech Message-ID: <7860C795470F49FE9270E6D5731619E1@LabHP> Hello Histonetters, This is a question for techs that are also Mohs techs. We are looking to hire a new ?back-up? Mohs tech, to cover my illnesses, and vacations. The question is if it would be more beneficial to have a tech that is local to us that is able to be trained by us, and step in a few times per year, or to hire someone from one of the firms that runs Mohs on a regular basis. Does anyone have experience with significant gaps in time between Mohs days? Is it ?like riding a bike?, where you can efficiently jump in? Thank you, Katie Riley Technical Supervisor of Dermatopathology Puyallup Dermatology Clinic 253-266-0799 katie at puyallupderm.com From melissa at alliedsearchpartners.com Wed Oct 4 15:09:47 2017 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Wed, 4 Oct 2017 20:09:47 +0000 Subject: [Histonet] Histology Supervisor/Manager Jobs (FL,VA,GA,CA,AL) Message-ID: Hello Histo Land, I have the following Supervisor/Manager openings in Histology. Please reach out to me for more details! They are all Full Time/Permanent. Histology Supervisor-1st Shift-Outside of Jacksonville-North/Central, FL Histology Supervisor-3rd Shift-Birmingham, AL Histology Supervisor-1st Shift-Atlanta, GA Histology Manager-1st Shift-Roanoke, VA Histology Supervisor-3rd Shift-Mission Viejo, CA Melissa Owens Allied Search Partners DL-407-413-9117 Toll Free: 888-388-7571 x. 102 melissa at alliedsearchpartners.com From alida.bailleul at gmail.com Wed Oct 4 15:33:32 2017 From: alida.bailleul at gmail.com (Alida Bailleul) Date: Wed, 4 Oct 2017 15:33:32 -0500 Subject: [Histonet] Methylene blue staining on paraffin slides Message-ID: Dear Histonet list, Where can I find the most standard protocol for Methylene blue staining on paraffin sections? Thank you so much All the best Alida Bailleul -- Alida Bailleul, PhD University of Missouri School of Medicine Columbia, MO, 65211 www.alidambailleul.com From Luis.Chiriboga at nyumc.org Thu Oct 5 07:11:43 2017 From: Luis.Chiriboga at nyumc.org (Chiriboga, Luis) Date: Thu, 5 Oct 2017 12:11:43 +0000 Subject: [Histonet] FASEB releases report based on results from the Shared Research Resources Survey Message-ID: <3E6798F00C9F494399E96B720ECD14292DE5FE2C@MSGWBDCPMBX32.nyumc.org> Dear Participants in FASEB's Shared Research Resources Survey, Today the Federation of American Societies for Experimental Biology (FASEB) released the report "Maximizing Shared Research Resources," which identifies the challenges encountered by shared research resources providers and users, and makes recommendations for improvement. Thank you again for participating in FASEB's Shared Research Resources Survey - your responses greatly informed this report. The survey results demonstrated the many benefits of shared resources, from cost efficiency to greater access to advanced technologies and services. However, respondents also described the many ways shared resources can fall short of their full potential. Based on the survey findings, FASEB identified four key areas for improvement: * Funding and Business Operations * Discoverability and Access; * Ability to Meet Evolving Needs; * Facility Career Track and Staff Development. In the accompanying recommendations, FASEB describes actions stakeholders - from sponsors to institutions, facilities, investigators, and scientific societies - can take to improve the deployment, use, and sustainability of shared resources. The full report, including the recommendations, survey analysis, and appendices (containing the summary results and additional resources) can be downloaded from this page. Please help us share this report! A communications toolkit can be downloaded here and provides summary text and sample social media posts. Please contact me if you have any questions or would like additional information. Thank you again for participating in FASEB's Shared Research Resources Survey! Bethany Bethany Drehman, PhD Senior Science Policy Analyst Office of Public Affairs Federation of American Societies for Experimental Biology 9650 Rockville Pike, Bethesda, MD 20814-3998 Email: bdrehman at faseb.org Phone: (301) 634-7659 Luis Chiriboga Ph.D, IHC Director DART Experimental Pathology NYU School of Medicine MSB 379 Office: 646-501-6934 Mobile: (347) 712-0897 Luis.Chiriboga at nyumc.org ------------------------------------------------------------ This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email. ================================= From criley at dpspa.com Thu Oct 5 07:17:52 2017 From: criley at dpspa.com (Charles Riley) Date: Thu, 5 Oct 2017 08:17:52 -0400 Subject: [Histonet] Expired antibodies Message-ID: Does anyone know any place that will buy or I could donate our expired antibodies? I just went through our refridgerator and cataloged all of the antibodies that have expired. We were using some for teaching purposes with the students who come through on rotations however due to budget needs we can no longer afford to allow them to run tests. Any suggestions would be greatly appreciated. -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From relia1 at earthlink.net Thu Oct 5 08:59:02 2017 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 5 Oct 2017 09:59:02 -0400 Subject: [Histonet] RELIA Special Job Alert for Managers and Supervisors 10-5-2017 Exciting opportunities Nationwide NC*FL*CA*VA*MA*AL* Message-ID: <007001d33de2$19d04860$4d70d920$@earthlink.net> Hello Histonetters, I have several exciting opportunities for experienced Managers, Supervisors and Lead Techs in hospital and private lab environments in several locations throughout the US. These are some of the premier employers in the United States. The positions are of course full time and permanent. My clients offer excellent compensation, benefits and relocation assistance. Here are my leadership positions: AP Manager - Charlotte, NC Mohs Histology Supervisor - Naples, FL Histology Supervisor - Salem, VA Histology Supervisor - Modesto, CA Histology Supervisor - Boston, MA Histology Supervisor - Birmingham, AL I anticipate multiple management openings with many exciting opportunities for talented managers and supervisor to be coming available nationwide as many of my clients are experiencing rapid growth again. If you are interested in looking into making a job change please let me know. That way I can keep you apprised of management opportunities that would interest you. Also if you would like more information or know of someone else who might be interested, please contact me via email at: relia1 at earthlink.net or toll free at: 866-607-3542 or on my cell at: 407-353-5070. I am available to discuss the opportunity at your convenience including after hours. Thanks-Pam There are a lot of recruiters out there right now trying to work with histology professionals and I appreciate your support and respect your needs. Remember I offer over 25 years of experience as a recruiter and for over 15 years I have dedicated my practice solely to placing histology professionals like you. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From elaineahoffman55 at yahoo.com Thu Oct 5 10:42:28 2017 From: elaineahoffman55 at yahoo.com (Elaine allison Hoffman) Date: Thu, 5 Oct 2017 15:42:28 +0000 (UTC) Subject: [Histonet] Old control blocks References: <1874905793.2076239.1507218148466.ref@mail.yahoo.com> Message-ID: <1874905793.2076239.1507218148466@mail.yahoo.com> Hi everyone, Just want to put a question out there. . . . How long can we keep control blocks (paraffin embedded tissue) before they go bad?? I just went through our old control blocks in our filing container and some of our control blocks have dates as far back as 10 years ago.? They have not?been refrigerated or anything but didn't know if they should be thrown out and start over?or?how long they are good for?? They are filed in alphabetical order, anything from amyloid controls to yeast control blocks and many others in-between.? Or?probably should be tested to see?just how positive the staining results turn out to be.? I was just wondering if anyone?knew off the top of their head, lol.? Another brain teaser.... Our cryostat is displaying a temperature of -23 degrees?but the thermometer?sitting inside the chamber is reading -15 degrees.? The thermometer was just recently purchased and does not need calibrated or anything.? Our supervisor thought we should?have a thermometer inside the cryo-chamber just?to be certain that the?digital display is accurate.? Now we have a dilemma!? Did anyone?else experience this problem with the temperature readings with their cryostat?? Not really sure what to do about the different readings.? What should the correct temperature be anyways? We?would really appreciate any feed-back or suggestions. Thank you, Elaine Hoffman, HT(ASCP) Steward Trumbull Memorial HospitalWarren, OH???? From Timothy.Morken at ucsf.edu Thu Oct 5 11:04:56 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 5 Oct 2017 16:04:56 +0000 Subject: [Histonet] Old control blocks In-Reply-To: <1874905793.2076239.1507218148466@mail.yahoo.com> References: <1874905793.2076239.1507218148466.ref@mail.yahoo.com> <1874905793.2076239.1507218148466@mail.yahoo.com> Message-ID: Elaine, blocks last a very long time. The at the face the tissue may be oxidized, like a section on a slide, but the deeper tissue should be good. If you have several extra blocks just coat the face with paraffin to protect it. However, the only way to be sure is to test them occasionally. If they are used regularly you can just note the last successful test (we keep a validation record of each block and note re-validation results on the form). If not used too often, test once a year to ensure it is good. Test the "extra paraffin-coated" blocks when they are needed, not every year - maybe when the previous block is nearing its end test the next block in line to be sure you have a control available. For the cryostat, for what part of the cryostat is the probe displaying a temperature? It may not be the chamber temperature. Our cryostat display (and controls) are for the knife and object holder. There is no built-in display for the chamber. We did put in a manual-read thermometer for the chamber, and all show different temperatures. The "correct" temperature is the one at which you get the best results! Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Elaine allison Hoffman via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, October 05, 2017 8:42 AM To: Histonetters Histonet Subject: [Histonet] Old control blocks Hi everyone, Just want to put a question out there. . . . How long can we keep control blocks (paraffin embedded tissue) before they go bad?? I just went through our old control blocks in our filing container and some of our control blocks have dates as far back as 10 years ago.? They have not?been refrigerated or anything but didn't know if they should be thrown out and start over?or?how long they are good for?? They are filed in alphabetical order, anything from amyloid controls to yeast control blocks and many others in-between.? Or?probably should be tested to see?just how positive the staining results turn out to be.? I was just wondering if anyone?knew off the top of their head, lol. Another brain teaser.... Our cryostat is displaying a temperature of -23 degrees?but the thermometer?sitting inside the chamber is reading -15 degrees.? The thermometer was just recently purchased and does not need calibrated or anything.? Our supervisor thought we should?have a thermometer inside the cryo-chamber just?to be certain that the?digital display is accurate.? Now we have a dilemma!? Did anyone?else experience this problem with the temperature readings with their cryostat?? Not really sure what to do about the different readings.? What should the correct temperature be anyways? We?would really appreciate any feed-back or suggestions. Thank you, Elaine Hoffman, HT(ASCP) Steward Trumbull Memorial HospitalWarren, OH _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz at premierlab.com Thu Oct 5 11:10:32 2017 From: liz at premierlab.com (Liz Chlipala) Date: Thu, 5 Oct 2017 16:10:32 +0000 Subject: [Histonet] Old control blocks In-Reply-To: <1874905793.2076239.1507218148466@mail.yahoo.com> References: <1874905793.2076239.1507218148466.ref@mail.yahoo.com> <1874905793.2076239.1507218148466@mail.yahoo.com> Message-ID: Elaine We have the temperature of our cryostat calibrated yearly by an outside calibration company. If the thermometer you are using inside the cryostat has been calibrated and is traceable I think you might have an issue with the temperature reading that is being displayed on your cryostat. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Elaine allison Hoffman via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, October 5, 2017 9:42 AM To: Histonetters Histonet Subject: [Histonet] Old control blocks Hi everyone, Just want to put a question out there. . . . How long can we keep control blocks (paraffin embedded tissue) before they go bad? I just went through our old control blocks in our filing container and some of our control blocks have dates as far back as 10 years ago. They have not been refrigerated or anything but didn't know if they should be thrown out and start over or how long they are good for? They are filed in alphabetical order, anything from amyloid controls to yeast control blocks and many others in-between. Or probably should be tested to see just how positive the staining results turn out to be. I was just wondering if anyone knew off the top of their head, lol. Another brain teaser.... Our cryostat is displaying a temperature of -23 degrees but the thermometer sitting inside the chamber is reading -15 degrees. The thermometer was just recently purchased and does not need calibrated or anything. Our supervisor thought we should have a thermometer inside the cryo-chamber just to be certain that the digital display is accurate. Now we have a dilemma! Did anyone else experience this problem with the temperature readings with their cryostat? Not really sure what to do about the different readings. What should the correct temperature be anyways? We would really appreciate any feed-back or suggestions. Thank you, Elaine Hoffman, HT(ASCP) Steward Trumbull Memorial HospitalWarren, OH _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From rhworkman at uro.com Thu Oct 5 12:17:41 2017 From: rhworkman at uro.com (Renee H. Workman) Date: Thu, 5 Oct 2017 17:17:41 +0000 Subject: [Histonet] Water Quality from Lab Gen Checklist distilled vs deionized vs reagent grade water Message-ID: Going over checklist needed input on water quality. We are a small lab and use distilled water purchased commercially. We do IHC but no special stains. We use the water in our water baths and for preparing IHC buffer. Should we upgrade to deionized or use distilled? Renee H. Workman Histology Supervisor Virginia Urology 9105 Stony Point Drive Richmond, VA 23235 W: 804-527-1316 | F: 804-270-0917 rhworkman at uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. From Timothy.Morken at ucsf.edu Thu Oct 5 13:01:14 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Thu, 5 Oct 2017 18:01:14 +0000 Subject: [Histonet] Water Quality from Lab Gen Checklist distilled vs deionized vs reagent grade water In-Reply-To: References: Message-ID: Renee, for practical purposes in histology they are pretty much the same. Note that the original water quality is key because certain things can carry over in each type of system. Commercial suppliers do all the filtering, conditioning and finishing for you. Deionized (DI) is now the usual water produced in institutions because it is easier to maintain and produces adequate amounts of water. Distillation requires a still and produces relatively small quantities compared to DI. In our histo lab we have a DI system, but it would be over-kill for a small lab. Buying distilled water will be fine. The company that makes distilled water does all the filtering and finishing for you so it is pure. If really concerned you can buy from a scientific supplier rather than a grocery store. In our EM lab we make up muscle histochemistry stains and with type 1 water we buy in 1 liter bottles. We have a DI system for everything else. This is a good article on the difference between DI and Dist. Water: http://www.labmanager.com/laboratory-technology/2014/09/the-right-water#.WdZwx4TyuUk Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Renee H. Workman via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, October 05, 2017 10:18 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Water Quality from Lab Gen Checklist distilled vs deionized vs reagent grade water Going over checklist needed input on water quality. We are a small lab and use distilled water purchased commercially. We do IHC but no special stains. We use the water in our water baths and for preparing IHC buffer. Should we upgrade to deionized or use distilled? Renee H. Workman Histology Supervisor Virginia Urology 9105 Stony Point Drive Richmond, VA 23235 W: 804-527-1316 | F: 804-270-0917 rhworkman at uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sandra.cheasty at wisc.edu Thu Oct 5 15:17:59 2017 From: sandra.cheasty at wisc.edu (Sandra Cheasty) Date: Thu, 05 Oct 2017 20:17:59 +0000 Subject: [Histonet] Grossing Blade for Ophthalmology Globes Message-ID: Hello all, Can someone recommend an economical disposable blade for grossing eye globes? Sharp enough to slice through sclera smoothly, strong enough to finish the cut through the lens. Cheers! Sandy Sandra J. Cheasty, HT (ASCP) Histology & Necropsy Supervisor UW-Madison, School of Veterinary Medicine From mneglia at trajanscimed.com Fri Oct 6 09:38:05 2017 From: mneglia at trajanscimed.com (Marc Neglia) Date: Sat, 7 Oct 2017 01:38:05 +1100 Subject: [Histonet] Job opportunity Message-ID: <61E7E1B920537D4BABCCDF8D8B6E70D1062B32B7AF7C@mifune1> Hello Histonetters, - Have you considered a move out of the lab and into the field? - Would you like to extend your reach to help many labs across the country achieve better patient outcomes? - Can you see yourself working for an innovative company whose primary mission is to improve the well-being of society? If you answered yes to any (or all) of the questions above, I've got a great opportunity for you. Trajan is looking to add a Product Specialist/Acct Mgr to our team in the US; the ideal person will have experience working in the lab as a histotech and/or supervisor and have a good understanding of the laboratory workflow. Please email me directly for details, if interested, and feel free to share with other colleagues that may interested. Best regards, Marc Marc Neglia Vice President of Sales, Americas Trajan Scientific and Medical Direct: 215 428 0501 Mobile: 609 238 6289 mneglia at trajanscimed.com Connect with me on LinkedIn [https://cdn.shopify.com/s/files/1/0767/9441/files/trajanlogoname.png?3631470203948037665] www.trajanscimed.com [https://cdn.shopify.com/s/files/1/0767/9441/files/twitter_icon_a2e6a682-9f9b-4d90-997d-3075b43703c3.jpg?3476987786659928520] [https://cdn.shopify.com/s/files/1/0767/9441/files/facebook_icon_6b1e9121-8d58-4826-ad01-8720fa562c98.jpg?3476987786659928520] [https://cdn.shopify.com/s/files/1/0767/9441/files/googleplus_icon_aef1634e-a89a-4884-b758-186e435c8a6a.jpg?3476987786659928520] [https://cdn.shopify.com/s/files/1/0767/9441/files/youtube_icon_bf677331-8669-490d-83db-75acd14949f4.jpg?3476987786659928520] [https://cdn.shopify.com/s/files/1/0767/9441/files/linkedin_icon_65fc35d2-2373-477e-9158-4401c0e99605.jpg?3476987786659928520] Read the latest news from Trajan Trajan Scientific and Medical | Trajan Scientific Americas Inc Wells Branch Tech Center - Austin TX, 1421 W Wells Branch Pkwy # 108, Pflugerville, Texas 78660, USA Toll Free: 800 945 6154 | Tel: 512 837 7190 | Fax: 512 836 9159 | usa at trajanscimed.com From eddessa at emory.edu Fri Oct 6 11:09:21 2017 From: eddessa at emory.edu (Dessasau III, Evan) Date: Fri, 6 Oct 2017 16:09:21 +0000 Subject: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain Message-ID: Hi Histonet , I have been trying to cut a FLAT 4 to 10 um frozen section from 50um sections of brain. Every time I think I have the tissue flat the sections are never in the same plane. I found a wonderful book in pdf format(A Practical Guide to Frozen Section Techniques, Stephen R. Peters) online with lots of wonderful tips but I'm having no luck implementing the tips. Has anyone ever tried this? Any help is GREATLY appreciated. Thank you, E-van E-van D. Dessasau, III, HTL(ASCP)cm Supervisor, Histology Division of Pathology Emory University Yerkes NPRC Main Center Rm. 2122 954 Gatewood Rd. Atlanta, GA. 30329 (404)727-7744 lab (404) 727-7902 office ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). From litepath2000 at yahoo.com Fri Oct 6 11:54:20 2017 From: litepath2000 at yahoo.com (LitePath) Date: Fri, 6 Oct 2017 16:54:20 +0000 (UTC) Subject: [Histonet] 2017 Region 1 Annual Meeting October 28-29 Portland ME References: <635258505.2866977.1507308860723.ref@mail.yahoo.com> Message-ID: <635258505.2866977.1507308860723@mail.yahoo.com> Hosted by Maine Societyfor HistotechnologyHoliday Inn by the BayPortland ME For more info & registration contact: Clare Thorntoncthornton at dahlchase.com From hhuggins at novalabs.co Fri Oct 6 12:30:53 2017 From: hhuggins at novalabs.co (Haley Huggins) Date: Fri, 6 Oct 2017 10:30:53 -0700 Subject: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain In-Reply-To: References: Message-ID: I would also be interested in knowing this tip if anyone knows it. I will also have to check out that book. We only do cryostat sections at our lab. *Haley Huggins, HT (ASCP)cm* *Technical Lab Supervisor* *1050 Las Tablas Rd, Suite 14* *Templeton, CA 93465* *Office: 877-230-1518* On Fri, Oct 6, 2017 at 9:09 AM, Dessasau III, Evan via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Histonet , I have been trying to cut a FLAT 4 to 10 um frozen section > from 50um sections of brain. Every time I think I have the tissue flat the > sections are never in the same plane. I found a wonderful book in pdf > format(A Practical Guide to Frozen Section Techniques, Stephen R. Peters) > online with lots of wonderful tips but I'm having no luck implementing the > tips. Has anyone ever tried this? Any help is GREATLY appreciated. > Thank you, > E-van > > E-van D. Dessasau, III, HTL(ASCP)cm > Supervisor, Histology Division of Pathology > Emory University > Yerkes NPRC > Main Center Rm. 2122 > 954 Gatewood Rd. > Atlanta, GA. 30329 > (404)727-7744 lab > (404) 727-7902 office > > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, distribution > or copying of this message (including any attachments) is strictly > prohibited. > > If you have received this message in error, please contact > the sender by reply e-mail message and destroy all copies of the > original message (including attachments). > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Timothy.Morken at ucsf.edu Fri Oct 6 12:36:42 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Fri, 6 Oct 2017 17:36:42 +0000 Subject: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain In-Reply-To: References: Message-ID: Haley, Maybe ask Stephen directly. He is on histonet, and has a website: https://www.pathologyinnovations.com/ Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Haley Huggins via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, October 06, 2017 10:31 AM To: Dessasau III, Evan Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain I would also be interested in knowing this tip if anyone knows it. I will also have to check out that book. We only do cryostat sections at our lab. *Haley Huggins, HT (ASCP)cm* *Technical Lab Supervisor* *1050 Las Tablas Rd, Suite 14* *Templeton, CA 93465* *Office: 877-230-1518* On Fri, Oct 6, 2017 at 9:09 AM, Dessasau III, Evan via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Histonet , I have been trying to cut a FLAT 4 to 10 um frozen > section from 50um sections of brain. Every time I think I have the > tissue flat the sections are never in the same plane. I found a > wonderful book in pdf format(A Practical Guide to Frozen Section > Techniques, Stephen R. Peters) online with lots of wonderful tips but > I'm having no luck implementing the tips. Has anyone ever tried this? Any help is GREATLY appreciated. > Thank you, > E-van > > E-van D. Dessasau, III, HTL(ASCP)cm > Supervisor, Histology Division of Pathology Emory University Yerkes > NPRC Main Center Rm. 2122 > 954 Gatewood Rd. > Atlanta, GA. 30329 > (404)727-7744 lab > (404) 727-7902 office > > > ________________________________ > > This e-mail message (including any attachments) is for the sole use of > the intended recipient(s) and may contain confidential and privileged > information. If the reader of this message is not the intended > recipient, you are hereby notified that any dissemination, > distribution or copying of this message (including any attachments) is > strictly prohibited. > > If you have received this message in error, please contact the sender > by reply e-mail message and destroy all copies of the original message > (including attachments). > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From eddessa at emory.edu Fri Oct 6 13:30:23 2017 From: eddessa at emory.edu (Dessasau III, Evan) Date: Fri, 6 Oct 2017 18:30:23 +0000 Subject: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain In-Reply-To: References: Message-ID: HI Ms. Powell!! , Thanks for the reply. When we last all saw each other at the Georgia Histopalooza I expressed an interest in training on the cryostat with Cindy Baranowski. She was so nice and had me come over to the hospital back in June. That was really really nice of her! But now I can't get the new Cryostat smell out of my nose! I have not reached out to her nor Jennifer nor Jeanine with this issue. I bother Jennifer all the time I try not to tread too much, she too is very helpful. There is another tech Susan Jenkins here at Yerkes that I have talk to about my project. She was doing something similar but found using the Vibratome worked for her project. I need sections thinner than the vibratome will cut. I'm using the Leica CM3050 S. I'm able to get the temp right to cut brain sections with little curl to them. I'm talking about getting my 50 um section(they are about 2 to 3cmsq) to remain flat through freezing so that I cut a section in one plane. I hope that makes a little more sense. I will reach out to Cindy. Thank you so much! E-van -----Original Message----- From: Shirley A. Powell [mailto:POWELL_SA at mercer.edu] Sent: Friday, October 06, 2017 1:41 PM To: Dessasau III, Evan Subject: RE: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain Hi Even, Have you asked any of the other histotechs at Emory about this. I know Cindy Baranowski, Jeanine Bartlett and Jennifer Smith work there. What cryostat are you using. I know that the temp for cutting brains is a little different from other tissues. Sometimes the machines have anti-roll systems in place too. Also How big are your brain sections? Shirley Powell -----Original Message----- From: Dessasau III, Evan via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, October 06, 2017 12:09 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain Hi Histonet , I have been trying to cut a FLAT 4 to 10 um frozen section from 50um sections of brain. Every time I think I have the tissue flat the sections are never in the same plane. I found a wonderful book in pdf format(A Practical Guide to Frozen Section Techniques, Stephen R. Peters) online with lots of wonderful tips but I'm having no luck implementing the tips. Has anyone ever tried this? Any help is GREATLY appreciated. Thank you, E-van E-van D. Dessasau, III, HTL(ASCP)cm Supervisor, Histology Division of Pathology Emory University Yerkes NPRC Main Center Rm. 2122 954 Gatewood Rd. Atlanta, GA. 30329 (404)727-7744 lab (404) 727-7902 office ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From litepath2000 at yahoo.com Fri Oct 6 13:37:19 2017 From: litepath2000 at yahoo.com (LitePath) Date: Fri, 6 Oct 2017 18:37:19 +0000 (UTC) Subject: [Histonet] CORRECTION Re: 2017 Region 1 Annual Meeting October 28-29 Portland ME In-Reply-To: <635258505.2866977.1507308860723@mail.yahoo.com> References: <635258505.2866977.1507308860723.ref@mail.yahoo.com> <635258505.2866977.1507308860723@mail.yahoo.com> Message-ID: <852549272.2959823.1507315039205@mail.yahoo.com> Corrected email address cthornton at dahlchase.com On Friday, October 6, 2017, 1:27:26 PM EDT, LitePath via Histonet wrote: Hosted by Maine Societyfor HistotechnologyHoliday Inn by the BayPortland ME For more info & registration contact: Clare Thorntoncthornton at dahlchase.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jford at cytomx.com Fri Oct 6 14:34:14 2017 From: jford at cytomx.com (Judi Ford) Date: Fri, 6 Oct 2017 19:34:14 +0000 Subject: [Histonet] looking for method for fixing frozen tissue Message-ID: Hi everyone, I was wondering if anyone can tell me the best way to formalin fix frozen tissue for processing into a paraffin block. I have tissue in a minus 80 freezer and would like to cut a piece from it to fix in formalin and process. I'm thinking that I could put the tissue into the cryostat and bring it slowly up to minus 20. The container of formalin could be cooled down so that both are near the same temperature when I submerge the tissue into the formalin. Are there other methods that may help process the tissue better? Huge thanks to any suggestions that come my way. Hope you all have a really good weekend. Regards, Judi Judi Gordon Senior Research Associate Translational Sciences/IHC CytomX Therapeutics STATEMENT OF CONFIDENTIALITY: The information contained in this electronic message and any attachments to this message are intended for the exclusive use of the addressee(s) and may contain confidential or privileged information. If you are not an intended recipient, or a person responsible for delivering the e-mail to an intended recipient, please be advised that you have received this message in error and that any use, dissemination, forwarding, printing, or copying is strictly prohibited. Please notify the sender at CytomX Therapeutics, Inc., immediately and destroy all copies of this message and any attachments. CytomX Therapeutics, Inc. From hhuggins at novalabs.co Fri Oct 6 14:56:53 2017 From: hhuggins at novalabs.co (Haley Huggins) Date: Fri, 6 Oct 2017 12:56:53 -0700 Subject: [Histonet] Developing a multiplex cocktail Message-ID: Hello Histonetters, It has been years since I had to create a multiplex of a dual-antibody cocktail and for the life of me am having a brain block on the best way to go about this. Does anyone have or know of a procedure to make this type of cocktail? *Haley Huggins, HT (ASCP)cm* *Technical Lab Supervisor* *1050 Las Tablas Rd, Suite 14* *Templeton, CA 93465* *Office: 877-230-1518* From eddessa at emory.edu Fri Oct 6 15:44:45 2017 From: eddessa at emory.edu (Dessasau III, Evan) Date: Fri, 6 Oct 2017 20:44:45 +0000 Subject: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain In-Reply-To: References: Message-ID: Hi Robert, thank you for your reply. So you froze the section on the aluminum with the "clock face" and then put this inverted, in the Microm 560 mold? ours is an older unit that has been around and not all the parts came with it so I don't have the 90 degree block chuck. Is this the fixed head you speak of? I love the idea of a control block for cutting I image you mean. I have been putting a layer of OCT on top of the block/section after I pop it out of the mold. I use this "extra" OCT to orientate my x-y. Robert how thick was the section you were trying to cut? I'm wondering if anyone knows if samples move about as the OCT freezes? Thank you again, E-van From: Jacox, Robert A. [mailto:robert.jacox at thermofisher.com] Sent: Friday, October 06, 2017 12:55 PM To: Dessasau III, Evan Cc: Siravo, Michele L. ; Cole, Alan D. Subject: RE: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain Evan, I had a similar project and did after a lot of trial and error was able to get consistent results. The way I approached it was the following. There are several variables that need to be accounted for. 1. Is the tissue cut on the plain I want to see (this has to be yes) 2. Mounting the section to a known flat surface 3. Attaching the section to the chuck in a consistent manner 4. The cutting angle of the specimen head 5. The placement of the knife 6. The accurate insertion of the knife/blade For point 2 I used a flat piece of aluminum and drew clock face marks on it. This way I knew I was consistently attaching the section on a known plain. Point 3 I used some embedding molds from the Microm 560 to act as a known way to attach the specimen. The molds end up making a bullet shaped sample so it was easy to keep orientation (full disclosure I would for Thermo (Microm) I would guess any consistent type mold will do). Point 4 I went from an adjustable head to a fixed head on the cryostat. Points 5 and 6 - I created a control block that I cut prior to beginning to cut and any time I moved the knife holder or changed the blade. For this I saved old 100 micron sections and would embed one using the Point 1-4 methodology and verify the plain. I hope this helps and let me know if you need further assistance Robert Jacox Commercial Marketing Manager Anatomic Pathology Thermo Fisher Scientific Tel: 269-544-5651 l Mobile: 269-598-0747 robert.jacox at thermofisher.com l www.thermoscientific.com -----Original Message----- From: Dessasau III, Evan via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, October 06, 2017 12:09 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain Hi Histonet , I have been trying to cut a FLAT 4 to 10 um frozen section from 50um sections of brain. Every time I think I have the tissue flat the sections are never in the same plane. I found a wonderful book in pdf format(A Practical Guide to Frozen Section Techniques, Stephen R. Peters) online with lots of wonderful tips but I'm having no luck implementing the tips. Has anyone ever tried this? Any help is GREATLY appreciated. Thank you, E-van E-van D. Dessasau, III, HTL(ASCP)cm Supervisor, Histology Division of Pathology Emory University Yerkes NPRC Main Center Rm. 2122 954 Gatewood Rd. Atlanta, GA. 30329 (404)727-7744 lab (404) 727-7902 office ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From robert.jacox at thermofisher.com Fri Oct 6 17:27:23 2017 From: robert.jacox at thermofisher.com (Jacox, Robert A.) Date: Fri, 6 Oct 2017 22:27:23 +0000 Subject: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain In-Reply-To: References: , Message-ID: <86676E64-C3D2-45C2-8FA9-720273667C8A@thermofisher.com> I just made the aluminum temple from a 1/2 inch 6x6 piece on aluminum from Home Depot. With the sheet you will este,Italy make it look like a clock face using a sharpe. Why you want to do this is i found the aluminum plates have enough variance that if I did not always use the same side it changed the plain of my cut. The key is to make sure once you established correctly you can repeat everything exactly the same. Sent from my iPad On Oct 6, 2017, at 4:44 PM, Dessasau III, Evan > wrote: Hi Robert, thank you for your reply. So you froze the section on the aluminum with the ?clock face? and then put this inverted, in the Microm 560 mold? ours is an older unit that has been around and not all the parts came with it so I don?t have the 90 degree block chuck. Is this the fixed head you speak of? I love the idea of a control block for cutting I image you mean. I have been putting a layer of OCT on top of the block/section after I pop it out of the mold. I use this ?extra? OCT to orientate my x-y. Robert how thick was the section you were trying to cut? I?m wondering if anyone knows if samples move about as the OCT freezes? Thank you again, E-van From: Jacox, Robert A. [mailto:robert.jacox at thermofisher.com] Sent: Friday, October 06, 2017 12:55 PM To: Dessasau III, Evan > Cc: Siravo, Michele L. >; Cole, Alan D. > Subject: RE: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain Evan, I had a similar project and did after a lot of trial and error was able to get consistent results. The way I approached it was the following. There are several variables that need to be accounted for. 1. Is the tissue cut on the plain I want to see (this has to be yes) 2. Mounting the section to a known flat surface 3. Attaching the section to the chuck in a consistent manner 4. The cutting angle of the specimen head 5. The placement of the knife 6. The accurate insertion of the knife/blade For point 2 I used a flat piece of aluminum and drew clock face marks on it. This way I knew I was consistently attaching the section on a known plain. Point 3 I used some embedding molds from the Microm 560 to act as a known way to attach the specimen. The molds end up making a bullet shaped sample so it was easy to keep orientation (full disclosure I would for Thermo (Microm) I would guess any consistent type mold will do). Point 4 I went from an adjustable head to a fixed head on the cryostat. Points 5 and 6 ? I created a control block that I cut prior to beginning to cut and any time I moved the knife holder or changed the blade. For this I saved old 100 micron sections and would embed one using the Point 1-4 methodology and verify the plain. I hope this helps and let me know if you need further assistance Robert Jacox Commercial Marketing Manager Anatomic Pathology Thermo Fisher Scientific Tel: 269-544-5651 l Mobile: 269-598-0747 robert.jacox at thermofisher.com l www.thermoscientific.com -----Original Message----- From: Dessasau III, Evan via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Friday, October 06, 2017 12:09 PM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain Hi Histonet , I have been trying to cut a FLAT 4 to 10 um frozen section from 50um sections of brain. Every time I think I have the tissue flat the sections are never in the same plane. I found a wonderful book in pdf format(A Practical Guide to Frozen Section Techniques, Stephen R. Peters) online with lots of wonderful tips but I'm having no luck implementing the tips. Has anyone ever tried this? Any help is GREATLY appreciated. Thank you, E-van E-van D. Dessasau, III, HTL(ASCP)cm Supervisor, Histology Division of Pathology Emory University Yerkes NPRC Main Center Rm. 2122 954 Gatewood Rd. Atlanta, GA. 30329 (404)727-7744 lab (404) 727-7902 office ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From eddessa at emory.edu Fri Oct 6 17:29:39 2017 From: eddessa at emory.edu (Dessasau III, Evan) Date: Fri, 6 Oct 2017 22:29:39 +0000 Subject: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain In-Reply-To: References: Message-ID: Hi Loralei, I will see If I can find someone with a sliding microtome. Are you using OCT to block the 50 um section? How do you ensure the section stays flat while blocking/freezing? Thank you for your reply ! E-van From: Loralei Dewe [mailto:lldewe at gmail.com] Sent: Friday, October 06, 2017 6:13 PM To: Dessasau III, Evan Subject: Re: [Histonet] FW: cutting a 5um frozen section from a 50um section of brain I would use a sliding microtome and freeze the 50 um sections solid with dry ice. I used to do sections of mouse brain that way. Loralei On Oct 6, 2017 9:10 AM, "Dessasau III, Evan via Histonet" > wrote: Hi Histonet , I have been trying to cut a FLAT 4 to 10 um frozen section from 50um sections of brain. Every time I think I have the tissue flat the sections are never in the same plane. I found a wonderful book in pdf format(A Practical Guide to Frozen Section Techniques, Stephen R. Peters) online with lots of wonderful tips but I'm having no luck implementing the tips. Has anyone ever tried this? Any help is GREATLY appreciated. Thank you, E-van E-van D. Dessasau, III, HTL(ASCP)cm Supervisor, Histology Division of Pathology Emory University Yerkes NPRC Main Center Rm. 2122 954 Gatewood Rd. Atlanta, GA. 30329 (404)727-7744 lab (404) 727-7902 office ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From yaziji at mac.com Sat Oct 7 19:11:42 2017 From: yaziji at mac.com (Hadi Yaziji) Date: Sat, 07 Oct 2017 20:11:42 -0400 Subject: [Histonet] Announcing the 12th Annual International Retreat on Applied IHC and Molecular Pathology - Key Largo, FL, USA Message-ID: Dear colleagues at Histonet, It is my pleasure to announce the early bird registration for the 12th annual AIMP retreat. Those of you who attended previous courses will receive a decent discount as specified on the website. Please let me know if you have any questions. I hope to see you in the FL Keyes next February. Best regards, Hadi Hadi Yaziji, MD, FCAP, FASCP Medical Director Vitro Molecular Laboratories 8700 W. Flagler Street, Suite 100 l Miami, FL 33174 www.vitromolecular.com hyaziji at vitromolecular.com Office 305-267-7979 l Fax 786-513-0175 12th Annual International Retreat on Applied Immunohistochemistry & Molecular Pathology Previous AIMP retreat attendees have attested that AIMP has been ?the best pathology CME event ever attended?. Our 12th annual retreat will have the following list of speakers (arranged alphabetically): Noah Brown, MD; University of Michigan Richard Cartun, PhD; Hartford Hospital Carol Cheung, MD, PhD., JD, FRCPC, University of Toronto Brenda Cox, FHFMA, CPC, MT (ASCP) Richard Eisen, MD; Banner Health Joel Greenson, MD; University of Michigan Jason Hornick, MD, PhD; Harvard University Jeffrey Myers, MD; University of Michigan Emina Terlacovich, MD, PhD; University of Saskatchewan Megan Troxell, MD; Stanford University Hadi Yaziji, MD, Vitro Molecular Laboratories Location: Key Largo Marriott (Key Largo, FL, USA) Date: February 4 - February 8, 2018 Topics at a glance: Sunday, February 4 Welcome and Opening Remarks [Hadi Yaziji] Pre-Test [Hadi Yaziji] Interpreting FISH Assays by The Practicing Pathologist - General Principles [Hadi Yaziji] Best of USCAP 2017: IHC Applications [Richard Eisen] Developing Fit-For-Purpose IHC Assays [Carol Cheung] Validation vs. Verification of IHC Assays: The Clue is in the Test Performance Characteristics [Emina Torlakovic] Tissue Tools are Power Tools for the IHC Laboratory. [Carol Cheung] Controlling the Controls in IHC ? a Path to Standardization [Emina Torlakovic] Monday, February 5: Malignant Mesothelioma and Other Diffuse Pleural Tumors [Jeffrey Myers] Role of the Pathologist in Diagnosis and Management of Patients with NSCLC [Jeffrey Myers] Interpreting FISH Assays: Common Assays & Specific Scenarios [Hadi Yaziji] Molecular Genetics of Colon Cancer, what a practicing surgical pathologist needs to know [Joel Greenson] Specimen Considerations for Solid Tumor Molecular Testing [Noah Brown] PD-L1: Interpretation Pitfalls & Update on Utility as a Companion Diagnostic Assay [Hadi Yaziji] Tuesday, February 6: HER2 Testing: 2018 ASCO/CAP Guidelines - What?s New and Why [Hadi Yaziji] Troubleshooting Diagnostic Immunohistochemistry [Rich Cartun] Open Mic: Bring Your Own Questions - Part I [Hadi Yaziji] Best ?Specialized? Markers (Myoepithelial, Cytokeratins, Neuroendocrine, Endothelial, Histocytic, Megakaryocitic, Erythroid, ETC) in IHC [Hadi Yaziji] Surgical Pathology Case Presentations - Session 1 [Faculty] Surgical Pathology Case Presentations - Session 2 [Faculty] Wednesday February 7: The Evolution of IHC for Soft Tissue Tumors in the 21st Century: From Differentiation to Molecular Genetics [Hornick] Immunohistochemistry in Kidney Tumors: The New WHO Classification and Beyond [Megan Troxell] Beyond Lineage: Diagnostic and Predictive Molecular IHC for Surgical Pathologists [Jason Hornick] Immunohistochemistry in Transplant Pathology [Megan Troxell] Surgical Pathology Case Presentations - Session 3 [Faculty] Surgical Pathology Case Presentations - Session 4 [Faculty] Thursday, February 8: Essential Coding & Compliance: 2018 Update - Part I [Brenda Cox] Essential Coding & Compliance: 2018 Update - Part II [Brenda Cox] Open Mic: Bring Your Own Questions - Part II [Hadi Yaziji] Open Mic: Bring Your Own Questions - Part III [Hadi Yaziji] Surgical Pathology Case Presentations - Session 5 [Faculty] Surgical Pathology Case Presentations - Session 6 [Faculty] To register for the retreat or for any other information, please visit us online at pathlearning.com For any other questions or inquiries, please email us at info at pathlearning.com For complete information of venue, retreat schedule, roster of expert speakers, we invite you to visit www.pathlearning.com . From Richard.Cartun at hhchealth.org Sat Oct 7 19:12:47 2017 From: Richard.Cartun at hhchealth.org (Cartun, Richard) Date: Sun, 8 Oct 2017 00:12:47 +0000 Subject: [Histonet] Announcing the 12th annual retreat of applied immunohistochemistry and molecular pathology at the beautiful Marriott, Key Largo, FL Message-ID: <9215BD4B0BA1B44D962A71C758B68D2E9546D65E@HHCEXCHMB03.hhcsystem.org> I am forwarding this exciting meeting announcement to the Histonet Community. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax Following pre-hurricane preparation and 2 weeks of lack of electricity post-hurricane, we?re finally able to get our lives back on track, hence this delayed announcement for the 12th annual AIMP retreat. Please forward this email to as many folks as you can. Those of you who attended previous retreats will receive a decent discount as specified on the website. Please let me know if you have any questions. I hope to see you in the FL Keyes in February. Best regards, Hadi Hadi Yaziji, MD, FCAP, FASCP Medical Director Vitro Molecular Laboratories 8700 W. Flagler Street, Suite 100 l Miami, FL 33174 www.vitromolecular.com hyaziji at vitromolecular.com Office 305-267-7979 l Fax 786-513-0175 [https://gallery.mailchimp.com/b4d3e9ff192b16fd68e3363a0/images/9e4a48fa-48d5-43fe-ac8c-f45b596f7767.jpg] 12th Annual International Retreat on Applied Immunohistochemistry & Molecular Pathology Previous AIMP retreat attendees have attested that AIMP has been ?the best pathology CME event ever attended?. Our 12th annual retreat will have the following list of speakers (arranged alphabetically): Noah Brown, MD; University of Michigan Richard Cartun, PhD; Hartford Hospital Carol Cheung, MD, PhD., JD, FRCPC, University of Toronto Brenda Cox, FHFMA, CPC, MT (ASCP) Richard Eisen, MD; Banner Health Joel Greenson, MD; University of Michigan Jason Hornick, MD, PhD; Harvard University Jeffrey Myers, MD; University of Michigan Emina Terlacovich, MD, PhD; University of Saskatchewan Megan Troxell, MD; Stanford University Hadi Yaziji, MD, Vitro Molecular Laboratories Location: Key Largo Marriott (Key Largo, FL, USA) Date: February 4 - February 8, 2018 Topics at a glance: Sunday, February 4 Welcome and Opening Remarks [Hadi Yaziji] Pre-Test [Hadi Yaziji] Interpreting FISH Assays by The Practicing Pathologist - General Principles [Hadi Yaziji] Best of USCAP 2017: IHC Applications [Richard Eisen] Developing Fit-For-Purpose IHC Assays [Carol Cheung] Validation vs. Verification of IHC Assays: The Clue is in the Test Performance Characteristics [Emina Torlakovic] Tissue Tools are Power Tools for the IHC Laboratory. [Carol Cheung] Controlling the Controls in IHC ? a Path to Standardization [Emina Torlakovic] Monday, February 5: Malignant Mesothelioma and Other Diffuse Pleural Tumors [Jeffrey Myers] Role of the Pathologist in Diagnosis and Management of Patients with NSCLC [Jeffrey Myers] Interpreting FISH Assays: Common Assays & Specific Scenarios [Hadi Yaziji] Molecular Genetics of Colon Cancer, what a practicing surgical pathologist needs to know [Joel Greenson] Specimen Considerations for Solid Tumor Molecular Testing [Noah Brown] PD-L1: Interpretation Pitfalls & Update on Utility as a Companion Diagnostic Assay [Hadi Yaziji] Tuesday, February 6: HER2 Testing: 2018 ASCO/CAP Guidelines - What?s New and Why [Hadi Yaziji] Troubleshooting Diagnostic Immunohistochemistry [Rich Cartun] Open Mic: Bring Your Own Questions - Part I [Hadi Yaziji] Best ?Specialized? Markers (Myoepithelial, Cytokeratins, Neuroendocrine, Endothelial, Histocytic, Megakaryocitic, Erythroid, ETC) in IHC [Hadi Yaziji] Surgical Pathology Case Presentations - Session 1 [Faculty] Surgical Pathology Case Presentations - Session 2 [Faculty] Wednesday February 7: The Evolution of IHC for Soft Tissue Tumors in the 21st Century: From Differentiation to Molecular Genetics [Hornick] Immunohistochemistry in Kidney Tumors: The New WHO Classification and Beyond [Megan Troxell] Beyond Lineage: Diagnostic and Predictive Molecular IHC for Surgical Pathologists [Jason Hornick] Immunohistochemistry in Transplant Pathology [Megan Troxell] Surgical Pathology Case Presentations - Session 3 [Faculty] Surgical Pathology Case Presentations - Session 4 [Faculty] Thursday, February 8: Essential Coding & Compliance: 2018 Update - Part I [Brenda Cox] Essential Coding & Compliance: 2018 Update - Part II [Brenda Cox] Open Mic: Bring Your Own Questions - Part II [Hadi Yaziji] Open Mic: Bring Your Own Questions - Part III [Hadi Yaziji] Surgical Pathology Case Presentations - Session 5 [Faculty] Surgical Pathology Case Presentations - Session 6 [Faculty] To register for the retreat or for any other information, please visit us online at pathlearning.com For any other questions or inquiries, please email us at info at pathlearning.com For complete information of venue, retreat schedule, roster of expert speakers, we invite you to visit www.pathlearning.com. -- -- You received this message because you are subscribed to the Google Groups "ihcrg" group. 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This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. From pruegghm at hotmail.com Mon Oct 9 12:19:15 2017 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Mon, 9 Oct 2017 17:19:15 +0000 Subject: [Histonet] Fw: Announcing the 12th annual retreat of applied immunohistochemistry and molecular pathology at the beautiful Marriott, Key Largo, FL In-Reply-To: References: Message-ID: Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 pruegghm at hotmail.com ________________________________ From: ancillarypath at mac.com Sent: Saturday, October 7, 2017 6:03 PM To: ihcrg ihcrg Subject: Announcing the 12th annual retreat of applied immunohistochemistry and molecular pathology at the beautiful Marriott, Key Largo, FL Dear colleagues at IHC Resource Group, Following pre-hurricane preparation and 2 weeks of lack of electricity post-hurricane, we?re finally able to get our lives back on track, hence this delayed announcement for the 12th annual AIMP retreat. Please forward this email to as many folks as you can. Those of you who attended previous retreats will receive a decent discount as specified on the website. Please let me know if you have any questions. I hope to see you in the FL Keyes in February. Best regards, Hadi Hadi Yaziji, MD, FCAP, FASCP Medical Director Vitro Molecular Laboratories 8700 W. Flagler Street, Suite 100 l Miami, FL 33174 www.vitromolecular.com Vitro Molecular Laboratories | Anatomic Pathology www.vitromolecular.com Vitro Molecular Laboratories provides routine and specialty anatomic pathology services to a loyal physician clientele. hyaziji at vitromolecular.com Office 305-267-7979 l Fax 786-513-0175 [https://gallery.mailchimp.com/b4d3e9ff192b16fd68e3363a0/images/9e4a48fa-48d5-43fe-ac8c-f45b596f7767.jpg] 12th Annual International Retreat on Applied Immunohistochemistry & Molecular Pathology Previous AIMP retreat attendees have attested that AIMP has been ?the best pathology CME event ever attended?. Our 12th annual retreat will have the following list of speakers (arranged alphabetically): Noah Brown, MD; University of Michigan Richard Cartun, PhD; Hartford Hospital Carol Cheung, MD, PhD., JD, FRCPC, University of Toronto Brenda Cox, FHFMA, CPC, MT (ASCP) Richard Eisen, MD; Banner Health Joel Greenson, MD; University of Michigan Jason Hornick, MD, PhD; Harvard University Jeffrey Myers, MD; University of Michigan Emina Terlacovich, MD, PhD; University of Saskatchewan Megan Troxell, MD; Stanford University Hadi Yaziji, MD, Vitro Molecular Laboratories Location: Key Largo Marriott (Key Largo, FL, USA) Date: February 4 - February 8, 2018 Topics at a glance: Sunday, February 4 Welcome and Opening Remarks [Hadi Yaziji] Pre-Test [Hadi Yaziji] Interpreting FISH Assays by The Practicing Pathologist - General Principles [Hadi Yaziji] Best of USCAP 2017: IHC Applications [Richard Eisen] Developing Fit-For-Purpose IHC Assays [Carol Cheung] Validation vs. Verification of IHC Assays: The Clue is in the Test Performance Characteristics [Emina Torlakovic] Tissue Tools are Power Tools for the IHC Laboratory. [Carol Cheung] Controlling the Controls in IHC ? a Path to Standardization [Emina Torlakovic] Monday, February 5: Malignant Mesothelioma and Other Diffuse Pleural Tumors [Jeffrey Myers] Role of the Pathologist in Diagnosis and Management of Patients with NSCLC [Jeffrey Myers] Interpreting FISH Assays: Common Assays & Specific Scenarios [Hadi Yaziji] Molecular Genetics of Colon Cancer, what a practicing surgical pathologist needs to know [Joel Greenson] Specimen Considerations for Solid Tumor Molecular Testing [Noah Brown] PD-L1: Interpretation Pitfalls & Update on Utility as a Companion Diagnostic Assay [Hadi Yaziji] Tuesday, February 6: HER2 Testing: 2018 ASCO/CAP Guidelines - What?s New and Why [Hadi Yaziji] Troubleshooting Diagnostic Immunohistochemistry [Rich Cartun] Open Mic: Bring Your Own Questions - Part I [Hadi Yaziji] Best ?Specialized? Markers (Myoepithelial, Cytokeratins, Neuroendocrine, Endothelial, Histocytic, Megakaryocitic, Erythroid, ETC) in IHC [Hadi Yaziji] Surgical Pathology Case Presentations - Session 1 [Faculty] Surgical Pathology Case Presentations - Session 2 [Faculty] Wednesday February 7: The Evolution of IHC for Soft Tissue Tumors in the 21st Century: From Differentiation to Molecular Genetics [Hornick] Immunohistochemistry in Kidney Tumors: The New WHO Classification and Beyond [Megan Troxell] Beyond Lineage: Diagnostic and Predictive Molecular IHC for Surgical Pathologists [Jason Hornick] Immunohistochemistry in Transplant Pathology [Megan Troxell] Surgical Pathology Case Presentations - Session 3 [Faculty] Surgical Pathology Case Presentations - Session 4 [Faculty] Thursday, February 8: Essential Coding & Compliance: 2018 Update - Part I [Brenda Cox] Essential Coding & Compliance: 2018 Update - Part II [Brenda Cox] Open Mic: Bring Your Own Questions - Part II [Hadi Yaziji] Open Mic: Bring Your Own Questions - Part III [Hadi Yaziji] Surgical Pathology Case Presentations - Session 5 [Faculty] Surgical Pathology Case Presentations - Session 6 [Faculty] To register for the retreat or for any other information, please visit us online at pathlearning.com For any other questions or inquiries, please email us at info at pathlearning.com For complete information of venue, retreat schedule, roster of expert speakers, we invite you to visit www.pathlearning.com. From relia1 at earthlink.net Tue Oct 10 09:48:58 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 10 Oct 2017 10:48:58 -0400 Subject: [Histonet] RELIA Histology Careers Bulletin 10-12-2017 The Flavors of Fall and EXCITING NEW CAREER OPPORTUNITIES! Message-ID: <3eb001d341d6$e7b63080$b7229180$@earthlink.net> Hello Histonetters, How are you? Are you enjoying the sights, sounds and flavors of Fall? I know I am enjoying college football after all I am a southern gal and you know how we southerners are about our Football Saturdays in the South. On the other hand I am still patiently waiting for that ?real Fall stuff?. You know like crisp cool air, beautiful fall colors, crunching leaves and steaming bowls of soup. As it still ?feels? like Summer here in Florida! What are you enjoying most of all about this season? Here is a list of my current openings. Please take a look and see if anything interests you or anyone you know! Remember if I hire someone you refer to me you will earn a referral bonus!! I Have Management Opportunities in: Florida ? Naples ? Brand New Position! Mohs experience required! North Carolina ? AP Manager opportunity in Charlotte! Virginia ? Run your own hospital lab near Roanoke, VA I have HT/HTL and eligible positions in: Texas ? Austin ? Cross train in IHC! North Carolina ? Raleigh AND Charlotte opportunities!!! Massachusetts ? IHC tech near Lexington, MA Tennessee ? Leading edge lab in Knoxville brand new client!! If you have another area in mind please drop me an email and let me know so that I can be on the lookout! I spend all day everyday looking at new job opportunities so you don?t have to! If you or anyone you know might be interested in any of these opportunities or would like help with a job search in another area of the USA please contact me. I can be reachedASAP on my cell or leave me a message to set up an appointment to chat at your convenience at relia1 at earthlink.net or toll free at 866-607-3542. Thank You! ? ? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From criley at dpspa.com Tue Oct 10 09:49:56 2017 From: criley at dpspa.com (Charles Riley) Date: Tue, 10 Oct 2017 10:49:56 -0400 Subject: [Histonet] Inventory purchasing question Message-ID: Does anyone use the Leica bond printers with labels and ribbons from another company? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs From rjr6 at psu.edu Tue Oct 10 10:16:41 2017 From: rjr6 at psu.edu (Roberta Horner) Date: Tue, 10 Oct 2017 15:16:41 +0000 Subject: [Histonet] research work Message-ID: <874a44b31f974e5eaf525df1b874692f@PSU.EDU> Is there anyone that does outside work for research that would be willing to share their prices? We haven't changed our prices for years and material costs are going up. We don't want to price too high. We are especially looking at prices for unstained slides for IHC using the plus slides. Roberta Horner Animal Diagnostic Lab Penn State University From Timothy.Morken at ucsf.edu Tue Oct 10 10:20:16 2017 From: Timothy.Morken at ucsf.edu (Morken, Timothy) Date: Tue, 10 Oct 2017 15:20:16 +0000 Subject: [Histonet] Inventory purchasing question In-Reply-To: References: Message-ID: Charles, we use General Data labels on the Cognitive printers (not sure if that is the printer you are using). We were using their 22 x 22 StainerShield for the Leica Bond as well as all histo labels. Then we switched to their StainerShield 24 x 22 "Ventana-style" label and use them on both Leica and Ventana stainers (both printed from our LIS to Cognitive printers at the microtome. We switched to ensure an uncomplicated workflow for all histo labels. These are used for all histo - H&E, SS, IHC, ISH). We also use General Data StainerShield 19x22 for another set of Leica Bond stainers that are not integrated with the LIS so are printed from the Leica software to a Cognitive printer. All work very well in all applications. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -----Original Message----- From: Charles Riley via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, October 10, 2017 7:50 AM To: Histo List Subject: [Histonet] Inventory purchasing question Does anyone use the Leica bond printers with labels and ribbons from another company? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen at parrishmed.com Wed Oct 11 06:00:23 2017 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Wed, 11 Oct 2017 07:00:23 -0400 Subject: [Histonet] Lab assistants Message-ID: <450B7A81EDA0C54E97C53D60F00776C32403B0C430@isexstore03> Good Morning, We are having a debate about what a Lab assistant can and can not do in the State of Florida. So, those of you in Florida please weigh in. Can they make alcohol dilutions and use those alcohols to: 1) Change an automatic stainer 2) Change the tissue processor What about recycling said alcohols? Are they allowed to pour formalin into the processor wells? Can they fill small bottles with formalin to be used by doctor's offices? The list could go on and on... but I will stop here. Thanks so much for any insight into this! Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.hannen at parrishmed.com www.parrishmed.com ====================================== "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" ====================================== From Jennifer.J.Schumacher at HealthPartners.Com Wed Oct 11 11:20:24 2017 From: Jennifer.J.Schumacher at HealthPartners.Com (Schumacher, Jennifer J) Date: Wed, 11 Oct 2017 16:20:24 +0000 Subject: [Histonet] Histology flooring Message-ID: <48c469cd4b2145b59efe0ac489a3eb33@HPEXCH01.HealthPartners.int> Hi Histonetters, We are looking to replace our flooring in Histology. Has anyone found a floor that they LOVE for Histology? Here are some of our challenges: 1) Floor cannot be slippery for pathologists/OR personnel/vendors who walk into the space in heels or wearing booties. 2) Histology needs to be able to scrape the floor to remove paraffin. 3) Histology needs to be able to identify tissue should it fall to the floor, inked or not inked. 4) Histology needs to be able to clean up small chemical spills, especially xylene, with it soaking into or melting the floor. Our housekeeping has significant staffing challenges, so an extensive housekeeping requirement to maintain the floors would not be an option for us. Thank you for your input (as specific as possible please). Jennifer ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 From CDavis at che-east.org Wed Oct 11 13:10:04 2017 From: CDavis at che-east.org (Cassie P. Davis) Date: Wed, 11 Oct 2017 18:10:04 +0000 Subject: [Histonet] Histology lab flooring In-Reply-To: References: Message-ID: Hi Jennifer, I've worked in five labs all with different flooring and the floor that is in my current lab seems to fit your needs. It is a battleship gray deck paint (with sand or grit in it) that was applied when the lab was built 1990. The gray hides the dirty paraffin, lets you see the black inked and the whitish un-inked specimens. We use sidewalk scrapers to get the excess paraffin up, sometime a little of the microtome cleaner too if it gets bad. We have surgeons bringing specimens down in their booties, office folks walking in their stilettos safely until they get on the smooth hall tiles just outside of our doors. Spills clean-up OK, we just haven't found anything to remove the mystery eosin stain that showed up one Monday but I don't think that's the floor's fault. happy hunting... Cassie Date: Wed, 11 Oct 2017 16:20:24 +0000 From: "Schumacher, Jennifer J" To: "'histonet at lists.utsouthwestern.edu'" Subject: [Histonet] Histology flooring Message-ID: <48c469cd4b2145b59efe0ac489a3eb33 at HPEXCH01.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" Hi Histonetters, We are looking to replace our flooring in Histology. Has anyone found a floor that they LOVE for Histology? Here are some of our challenges: 1) Floor cannot be slippery for pathologists/OR personnel/vendors who walk into the space in heels or wearing booties. 2) Histology needs to be able to scrape the floor to remove paraffin. 3) Histology needs to be able to identify tissue should it fall to the floor, inked or not inked. 4) Histology needs to be able to clean up small chemical spills, especially xylene, with it soaking into or melting the floor. Our housekeeping has significant staffing challenges, so an extensive housekeeping requirement to maintain the floors would not be an option for us. Thank you for your input (as specific as possible please). Jennifer Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From ASelf at tidelandshealth.org Thu Oct 12 08:42:19 2017 From: ASelf at tidelandshealth.org (Amy Self) Date: Thu, 12 Oct 2017 09:42:19 -0400 Subject: [Histonet] Competency Assessment Message-ID: Good Morning Everyone, Does anyone have a competency assessment with the six elements of competency included that they would be willing to share with me? I have not been the best at keeping up with my department's competency and now we have a new lab director and he is asking that I improve what I have in place because I currently do not list the six elements that is being required by CAP. Thanks in advance and Happy Thursday because it's connected to Friday.. :) Amy Self Histology Lab Senior Tech Lab Tidelands Georgetown Memorial Hospital 606 Black River Road Georgetown, SC 29440 843-520-8711 ASelf at tidelandshealth.org Our mission: We help people live better lives through better health. NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From jaylundgren at gmail.com Thu Oct 12 15:04:23 2017 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 12 Oct 2017 13:04:23 -0700 Subject: [Histonet] Lab assistants In-Reply-To: <450B7A81EDA0C54E97C53D60F00776C32403B0C430@isexstore03> References: <450B7A81EDA0C54E97C53D60F00776C32403B0C430@isexstore03> Message-ID: The most important role of the lab assistant in the Histology laboratory is to be the target of middle management ire. Sincerely, Jay A. Lundgren, M.S., HTL(ASCP) On Wed, Oct 11, 2017 at 4:00 AM, Hannen, Valerie via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good Morning, We are having a debate about what a Lab assistant can and > can not do in the State of Florida. So, those of you in Florida please > weigh in. Can they make alcohol dilutions and use those alcohols to: > > 1) Change an automatic stainer > > 2) Change the tissue processor > > > What about recycling said alcohols? Are they allowed to pour formalin > into the processor wells? Can they fill small bottles with formalin to be > used by doctor's offices? The list could go on and on... but I will stop > here. > > Thanks so much for any insight into this! > > > Valerie Hannen,MLT(ASCP),HTL,SU (FL) > Section Chief, Histology > Parrish Medical Center > 951 N. Washington Ave. > Titusville,Florida 32796 > T: (321)268-6333 ext. 7506 > F: (321) 268-6149 > valerie.hannen at parrishmed.com > www.parrishmed.com > > ====================================== > "This email is intended solely for the use of the individual to > whom it is addressed and may contain information that is > privileged, confidential or otherwise exempt from disclosure > under applicable law. If the reader of this email is not the > intended recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution, or > copying of this communication is strictly prohibited. If you > have received this communication in error, please immediately > delete this message. Thank you" > ====================================== > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From c.tague at Pathologyarts.com Thu Oct 12 15:23:10 2017 From: c.tague at Pathologyarts.com (Curt) Date: Thu, 12 Oct 2017 20:23:10 +0000 Subject: [Histonet] IHC counterstain offline Message-ID: <9C8F910F72893643B3C3793C3D67132B68AD7E32@PATHOLOGYSERVER.pathologyarts.local> We currently use the benchmark ultra and are, in an effort to save a little money, looking at running out counter stain offline. Anyone have any suggestions or thoughts they might offer? We currently run our H&E with Richard Allen Hematoxylin 7111. I am curious if that is suitable for use in your experience or if you recommend a different Hematoxylin for IHC counter stains. How much bluing time, etc. Thanks, Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. From bcooper at chla.usc.edu Thu Oct 12 15:24:49 2017 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Thu, 12 Oct 2017 20:24:49 +0000 Subject: [Histonet] Lab assistants In-Reply-To: References: <450B7A81EDA0C54E97C53D60F00776C32403B0C430@isexstore03> Message-ID: Too funny Jay! I take it you've met my old boss!! LOL! Brian -----Original Message----- From: Jay Lundgren via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Thursday, October 12, 2017 1:04 PM To: Hannen, Valerie Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Lab assistants (EXTERNAL EMAIL) The most important role of the lab assistant in the Histology laboratory is to be the target of middle management ire. Sincerely, Jay A. Lundgren, M.S., HTL(ASCP) On Wed, Oct 11, 2017 at 4:00 AM, Hannen, Valerie via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good Morning, We are having a debate about what a Lab assistant can and > can not do in the State of Florida. So, those of you in Florida > please weigh in. Can they make alcohol dilutions and use those alcohols to: > > 1) Change an automatic stainer > > 2) Change the tissue processor > > > What about recycling said alcohols? Are they allowed to pour formalin > into the processor wells? Can they fill small bottles with formalin to > be used by doctor's offices? The list could go on and on... but I > will stop here. > > Thanks so much for any insight into this! > > > Valerie Hannen,MLT(ASCP),HTL,SU (FL) > Section Chief, Histology > Parrish Medical Center > 951 N. Washington Ave. > Titusville,Florida 32796 > T: (321)268-6333 ext. 7506 > F: (321) 268-6149 > valerie.hannen at parrishmed.com > www.parrishmed.com > > ====================================== > "This email is intended solely for the use of the individual to whom > it is addressed and may contain information that is privileged, > confidential or otherwise exempt from disclosure under applicable law. > If the reader of this email is not the intended recipient or the > employee or agent responsible for delivering the message to the > intended recipient, you are hereby notified that any dissemination, > distribution, or copying of this communication is strictly prohibited. > If you have received this communication in error, please immediately > delete this message. Thank you" > ====================================== > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From bakevictoria at gmail.com Thu Oct 12 16:07:46 2017 From: bakevictoria at gmail.com (Victoria Baker) Date: Thu, 12 Oct 2017 17:07:46 -0400 Subject: [Histonet] IHC counterstain offline In-Reply-To: <9C8F910F72893643B3C3793C3D67132B68AD7E32@PATHOLOGYSERVER.pathologyarts.local> References: <9C8F910F72893643B3C3793C3D67132B68AD7E32@PATHOLOGYSERVER.pathologyarts.local> Message-ID: Curt, I am not sure how much you will actually save if you factor in the cost of a person hand counter staining these slides. I will be the first to say Ventana is over priced, but off line counter staining brings in an additional variable and cost. In the end is it a real savings? It's an individual call, but I wouldn't do it. Vikki On Oct 12, 2017 4:43 PM, "Curt via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > We currently use the benchmark ultra and are, in an effort to save a > little money, looking at running out counter stain offline. Anyone have any > suggestions or thoughts they might offer? We currently run our H&E with > Richard Allen Hematoxylin 7111. I am curious if that is suitable for use in > your experience or if you recommend a different Hematoxylin for IHC counter > stains. How much bluing time, etc. > > > > Thanks, > > Curt > > > CONFIDENTIALITY NOTE: The information transmitted, including attachments, > is intended only for the person(s) or entity to which it is addressed and > may contain confidential and/or privileged material. Any review, > retransmission, dissemination or other use of, or taking of any action in > reliance upon this information by persons or entities other than the > intended recipient is prohibited. If you received this in error, please > contact the sender and destroy any copies of this information. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From NRich at bmhsc.org Fri Oct 13 13:07:31 2017 From: NRich at bmhsc.org (Nina J. Rich) Date: Fri, 13 Oct 2017 18:07:31 +0000 Subject: [Histonet] cpt codes Message-ID: Does anyone use CPT code modifiers to recoup non billable charges such as when we go to other departments to collect specimen like bone marrows and also for tech time for send outs. If not modifiers how do you charge for these services? Thanks Jean From lindsey.s.fairbourn at aruplab.com Mon Oct 16 14:28:46 2017 From: lindsey.s.fairbourn at aruplab.com (Fairbourn, Lindsey S.) Date: Mon, 16 Oct 2017 19:28:46 +0000 Subject: [Histonet] QIHC Study Materials Message-ID: <8d119402cfe24e149dab8c664e3bae67@aruplab.com> Hello Histonet, What is the best textbook to use to study for the QIHC exam? Thanks for your help! Lindsey ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From jaylundgren at gmail.com Tue Oct 17 04:32:15 2017 From: jaylundgren at gmail.com (Jay Lundgren) Date: Tue, 17 Oct 2017 02:32:15 -0700 Subject: [Histonet] QIHC Study Materials In-Reply-To: <8d119402cfe24e149dab8c664e3bae67@aruplab.com> References: <8d119402cfe24e149dab8c664e3bae67@aruplab.com> Message-ID: The free one from Dako. Virus-free. www.avg.com <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> On Mon, Oct 16, 2017 at 12:28 PM, Fairbourn, Lindsey S. via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello Histonet, > > What is the best textbook to use to study for the QIHC exam? > > Thanks for your help! > Lindsey > > ------------------------------------------------------------------- > The information transmitted by this e-mail and any included > attachments are from ARUP Laboratories and are intended only for the > recipient. The information contained in this message is confidential > and may constitute inside or non-public information under > international, federal, or state securities laws, or protected health > information and is intended only for the use of the recipient. > Unauthorized forwarding, printing, copying, distributing, or use of > such information is strictly prohibited and may be unlawful. If you > are not the intended recipient, please promptly delete this e-mail > and notify the sender of the delivery error or you may call ARUP > Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 > (800) 522-2787 ext. 2100 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mverdu at histopat.es Tue Oct 17 05:12:32 2017 From: mverdu at histopat.es (=?UTF-8?Q?Montse_Verd=C3=BA?=) Date: Tue, 17 Oct 2017 12:12:32 +0200 Subject: [Histonet] Tissue-Tek VIP 6 AI Vacuum Infiltration Processor Message-ID: Dear histonetters, Does anyone experience with de Tissue-Tek VIP 6 AI vacuum infiltration procesor? How often do you experience problems with these instruments that you need to have service for them? What kind of problems are they? Thank you in advance! Montse Verd? Histopat laboratoris Tel +34932033000 Biopat. Biopatologia Molecular Tel+34932030837 mverdu at histopat.es Aquest missatge i els seus annexos estan adre?ats exclusivament a la persona o entitat que figura com a destinatari i poden contenir dades i/o informaci? confidencial, sotmeses a secret professional o la divulgaci? de les quals estigui prohibida en virtut de la legislaci? vigent. Tota distribuci?, divulgaci? o reproducci? de aquesta comunicaci? per part de persones o entitats diferents del destinatari est? prohibida. Si ha rebut aquest missatge per error, si-us-plau, contacti amb la persona que figura com a remitent i procedeixi a la seva eliminaci?. Este mensaje y sus anexos van dirigidos exclusivamente a la persona o entidad que figura como destinatario, y pueden contener datos y/o informaci?n confidencial, sometidos a secreto profesional o cuya divulgaci?n est? prohibida en virtud de la legislaci?n vigente. Toda distribuci?n, divulgaci?n o reproducci?n de esta comunicaci?n por parte de personas o entidades distintas al destinatario est? prohibida. Si ha recibido este mensaje por error, por favor, contacte con la persona que figura como remitente y proceda a su eliminaci?n. This message and its attachments are addressed exclusively to the person or entity indicated as its named addressee, and may contain data and/or confidential information, subject to professional secret or whose disclosure is prohibited by virtue of current legislation in force. Any distribution, disclosure or reproduction of this type by persons or entities other than the addressee is strictly prohibited. If you have received this message by error, please contact the person indicated as its sender and delete it. From mverdu at histopat.es Tue Oct 17 05:36:25 2017 From: mverdu at histopat.es (=?UTF-8?Q?Montse_Verd=C3=BA?=) Date: Tue, 17 Oct 2017 12:36:25 +0200 Subject: [Histonet] RV: Tissue-Tek VIP 6 AI Vacuum Infiltration Processor In-Reply-To: f950a6aa685f8bab8e409df827956369@mail.gmail.com References: f950a6aa685f8bab8e409df827956369@mail.gmail.com Message-ID: Dear histonetters, Does anyone experience with the Tissue-Tek VIP 6 AI vacuum infiltration procesor? How often do you experience problems with these instruments that you need to have service for them? What kind of problems are they? Thank you in advance! Montse Verd? Histopat laboratoris Tel +34932033000 Biopat. Biopatologia Molecular Tel+34932030837 mverdu at histopat.es Aquest missatge i els seus annexos estan adre?ats exclusivament a la persona o entitat que figura com a destinatari i poden contenir dades i/o informaci? confidencial, sotmeses a secret professional o la divulgaci? de les quals estigui prohibida en virtut de la legislaci? vigent. Tota distribuci?, divulgaci? o reproducci? de aquesta comunicaci? per part de persones o entitats diferents del destinatari est? prohibida. Si ha rebut aquest missatge per error, si-us-plau, contacti amb la persona que figura com a remitent i procedeixi a la seva eliminaci?. Este mensaje y sus anexos van dirigidos exclusivamente a la persona o entidad que figura como destinatario, y pueden contener datos y/o informaci?n confidencial, sometidos a secreto profesional o cuya divulgaci?n est? prohibida en virtud de la legislaci?n vigente. Toda distribuci?n, divulgaci?n o reproducci?n de esta comunicaci?n por parte de personas o entidades distintas al destinatario est? prohibida. Si ha recibido este mensaje por error, por favor, contacte con la persona que figura como remitente y proceda a su eliminaci?n. This message and its attachments are addressed exclusively to the person or entity indicated as its named addressee, and may contain data and/or confidential information, subject to professional secret or whose disclosure is prohibited by virtue of current legislation in force. Any distribution, disclosure or reproduction of this type by persons or entities other than the addressee is strictly prohibited. If you have received this message by error, please contact the person indicated as its sender and delete it. From mverdu at histopat.es Tue Oct 17 05:48:25 2017 From: mverdu at histopat.es (=?UTF-8?Q?Montse_Verd=C3=BA?=) Date: Tue, 17 Oct 2017 12:48:25 +0200 Subject: [Histonet] coverslipper Dako question Message-ID: <73b613a754e9205a41d06905f887e81e@mail.gmail.com> Dear histonetters, Does anyone experience with the Coverslipper Dako? How often do you experience problems with these instruments that you need to have service for them? What kind of problems are they? In our case the drawer is not securely attached, the magnet is not securely attached to the equipment so the basket collides with the drawer and breaks the slides. Does anyone else have this same problem? Thank you in advance! Montse Verd? Histopat laboratoris Tel +34932033000 Biopat. Biopatologia Molecular Tel+34932030837 mverdu at histopat.es Aquest missatge i els seus annexos estan adre?ats exclusivament a la persona o entitat que figura com a destinatari i poden contenir dades i/o informaci? confidencial, sotmeses a secret professional o la divulgaci? de les quals estigui prohibida en virtut de la legislaci? vigent. Tota distribuci?, divulgaci? o reproducci? de aquesta comunicaci? per part de persones o entitats diferents del destinatari est? prohibida. Si ha rebut aquest missatge per error, si-us-plau, contacti amb la persona que figura com a remitent i procedeixi a la seva eliminaci?. Este mensaje y sus anexos van dirigidos exclusivamente a la persona o entidad que figura como destinatario, y pueden contener datos y/o informaci?n confidencial, sometidos a secreto profesional o cuya divulgaci?n est? prohibida en virtud de la legislaci?n vigente. Toda distribuci?n, divulgaci?n o reproducci?n de esta comunicaci?n por parte de personas o entidades distintas al destinatario est? prohibida. Si ha recibido este mensaje por error, por favor, contacte con la persona que figura como remitente y proceda a su eliminaci?n. This message and its attachments are addressed exclusively to the person or entity indicated as its named addressee, and may contain data and/or confidential information, subject to professional secret or whose disclosure is prohibited by virtue of current legislation in force. Any distribution, disclosure or reproduction of this type by persons or entities other than the addressee is strictly prohibited. If you have received this message by error, please contact the person indicated as its sender and delete it. From Kristopher.Kalleberg at unilever.com Tue Oct 17 08:17:50 2017 From: Kristopher.Kalleberg at unilever.com (Kalleberg, Kristopher) Date: Tue, 17 Oct 2017 13:17:50 +0000 Subject: [Histonet] Qiagen Qproteome FFPE Tissue Kit Message-ID: Hello, Has anyone tried the Qproteome FFPE Tissue Kit kit from Qiagen: Qproteome FFPE Tissue Kit For isolation of full-length proteins from formalin-fixed tissues * Standardized, quantitative extraction from FFPE tissue * Full-length proteins * Low sample requirements The Qproteome FFPE Tissue Kit uses innovative proprietary chemistry to reverse the crosslinking process of formalin fixation and deliver full-length proteins ready for subsequent analysis. Just curious if these kits are truly able to allow for isolation of proteins from formalin fixed tissue. I know this technology has been used for a number of years manually, just wondering how these kits are to manual methods and if it can be used for all proteins or only a certain proteins or sizes. Thank you in advance. Kris IMPORTANT NOTICE: This email and any attachments may contain information that is confidential and privileged. It is intended to be received only by persons entitled to receive the information. If you are not the intended recipient, please delete it from your system and notify the sender. You should not copy it or use it for any purpose nor disclose or distribute its contents to any other person. From hhuggins at novalabs.co Tue Oct 17 10:38:23 2017 From: hhuggins at novalabs.co (Haley Huggins) Date: Tue, 17 Oct 2017 08:38:23 -0700 Subject: [Histonet] QIHC Study Materials In-Reply-To: References: <8d119402cfe24e149dab8c664e3bae67@aruplab.com> Message-ID: I sent to Lindsey in a private email which I thought I had hit reply all that Michigan Society of Histotechnology also has a study guide for the IHC, but you are right, Jay, that the Dako IHC manual is your best study guide. *Haley Huggins, HT (ASCP)cm* *Technical Lab Supervisor* *1050 Las Tablas Rd, Suite 14* *Templeton, CA 93465* *Office: 877-230-1518* On Tue, Oct 17, 2017 at 2:32 AM, Jay Lundgren via Histonet < histonet at lists.utsouthwestern.edu> wrote: > The free one from Dako. > > utm_source=link&utm_campaign=sig-email&utm_content=webmail> > Virus-free. > www.avg.com > utm_source=link&utm_campaign=sig-email&utm_content=webmail> > <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> > > On Mon, Oct 16, 2017 at 12:28 PM, Fairbourn, Lindsey S. via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hello Histonet, > > > > What is the best textbook to use to study for the QIHC exam? > > > > Thanks for your help! > > Lindsey > > > > ------------------------------------------------------------------- > > The information transmitted by this e-mail and any included > > attachments are from ARUP Laboratories and are intended only for the > > recipient. The information contained in this message is confidential > > and may constitute inside or non-public information under > > international, federal, or state securities laws, or protected health > > information and is intended only for the use of the recipient. > > Unauthorized forwarding, printing, copying, distributing, or use of > > such information is strictly prohibited and may be unlawful. If you > > are not the intended recipient, please promptly delete this e-mail > > and notify the sender of the delivery error or you may call ARUP > > Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 > > (800) 522-2787 ext. 2100 > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 at earthlink.net Tue Oct 17 10:58:21 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 17 Oct 2017 11:58:21 -0400 Subject: [Histonet] ICYMI: Check it out my second "Make the Cut" column on Interviewing Tips for Histologists in the NSH Fall 2017 Career Center Newsletter! 10/17/2017 Message-ID: <000001d34760$c1f70420$45e50c60$@earthlink.net> Hi Histonetters, ICYMI - In case you missed it!! I am so excited!! My Second article has been published in the NSH Quarterly Career Center Newsletter under my new byline: Make the Cut I will be writing for the NSH Career newsletter on a quarterly basis and a few months ago in one of my emails I asked for topics of interest in anticipation of this column. Thanks again for the suggestions and please keep them coming!! The first column was about Salary Negotiations and the second one is Interviewing Tips for Histologists. Here is the link to both articles: http://nsh.org/content/career-center-newsletter If you have a minute to read it I would love to hear what you think. I also have some exciting job opportunities to pass along. One of these could give you the chance to try out those interviewing tips for histologists!! ? Please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral reward! I LOVE TO GIVE REFERRAL REWARDS!! SOME of these are RELIA exclusives? MOST of these offer Sign- on Bonuses and/or Relocation Assistance ALL of these Companies offer excellent compensation, benefits and great environments, opportunity for growth and great people to work with. AND THEY ALL ARE READY TO HIRE!! What more could you ask for? If You Or Anyone You Know Might Be Interested In A New Opportunity Please Contact Me. If you want to chat ASAP call or text me on my cell at 407-353-5070. If you want some additional information or to set up a time to chat please call me toll free at 866-607-3542 or email me at relia1 at earthlink.net Here are some of our Hottest Opportunities: We have leadership opportunities in: Florida, Virginia and North Carolina We have Histotechnician/Histotechnologist opportunities in: California, Texas, Tennessee, New York and North Carolina We have IHC Specialist Positions in: North Carolina and Massachusetts And my phone is still ringing off the hook so if you don?t see the location you want yet drop me a line so I can let you know when something DOES come up. I could be talking to a client about your next opportunity RIGHT NOW!! Have a great day. I look forward to hearing back from you. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From sprice2003 at gmail.com Tue Oct 17 17:00:42 2017 From: sprice2003 at gmail.com (Sally Price) Date: Tue, 17 Oct 2017 18:00:42 -0400 Subject: [Histonet] Histonet Digest, Vol 167, Issue 12 In-Reply-To: References: Message-ID: All: I beg to differ with what seems to be the general consensus, but the Dako handbook is not really representative of the available technology within the IHC field and shouldn't be thought of as a prescription for success. All the Dako book addresses is Dako's methods, reagents and instruments, which is pretty biased. In order to properly prepare for the ASCPs exam, one needs to think beyond reagents and automated systems and REALLY understand the underlying science. I've attended a number of seminars on this subject and learned that, especially because of recent changes to this test, one also needs to understand the clinical application of these procedures. More and more we're called upon to provide pathologists with support in the way of procedure validation and troubleshooting and we need to learn a great deal more than can be learned from reading one vendor's publication. +Sally From hhuggins at novalabs.co Tue Oct 17 17:29:02 2017 From: hhuggins at novalabs.co (Haley Huggins) Date: Tue, 17 Oct 2017 15:29:02 -0700 Subject: [Histonet] Histonet Digest, Vol 167, Issue 12 In-Reply-To: References: Message-ID: My suggestion for using the Dako IHC manual is not necessarily just about the automated application, but about the antibodies themselves and what the end results are supposed to be. It isn't the only thing you should use to prepare for the QIHC exam, but if you want to learn about a large amount of antibodies and what they target, the Dako IHC manual is the book of choice. I have a friend that took the exam a few years back and that was her suggestion as well. Yes you should know about the different platforms, but the use of the antibodies in IHC staining will still be used to get the targeted results provided in the Dako manual. The other platform manuals do not provide the same detailed descriptions of the antibodies and their purposes as the Dako manual does. In my lab, we run our IHC by hand, so I don't use any of the automated platforms that are available today, but luckily I know how to use most if not all of them from previous employment. Pull from your experiences with IHC, read up from all the platforms, use the Dako manual as a guide for a large variety of the antibodies, and check out the study guide from Michigan Society of HIstotechnology. If you are lucky attend a seminar on preparing for the exam. I have taken a couple of them, they can be helpful. Haley *Haley Huggins, HT (ASCP)cm* *Technical Lab Supervisor* *1050 Las Tablas Rd, Suite 14* *Templeton, CA 93465* *Office: 877-230-1518* On Tue, Oct 17, 2017 at 3:00 PM, Sally Price via Histonet < histonet at lists.utsouthwestern.edu> wrote: > All: > I beg to differ with what seems to be the general consensus, but the Dako > handbook is not really representative of the available technology within > the IHC field and shouldn't be thought of as a prescription for > success. All the Dako book addresses is Dako's methods, reagents and > instruments, which is pretty biased. In order to properly prepare for the > ASCPs exam, one needs to think beyond reagents and automated systems and > REALLY understand the underlying science. I've attended a number of > seminars on this subject and learned that, especially because of recent > changes to this test, one also needs to understand the clinical application > of these procedures. More and more we're called upon to provide > pathologists with support in the way of procedure validation and > troubleshooting and we need to learn a great deal more than can be learned > from reading one vendor's publication. > +Sally > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From michael.gudo at morphisto.de Wed Oct 18 05:54:25 2017 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Wed, 18 Oct 2017 12:54:25 +0200 Subject: [Histonet] Unused TissueFaxs Analysis-System (TissueQuest & HistQuest) Message-ID: <5A17A511-5D8F-40A1-87D7-BAC6883814C6@morphisto.de> Hello Histonetters, I am not sure if this question reaches the right persons here, but I try to place my question here: We have purchased a complete TissueFaxs-Akquisition and Analysis-System from TissueGnostics a few years ago for a research project. However, the project is finished for about 1 or 2 years, and now the Analysis System (TissueQuest and HistoQuest) will be not used here any more. I think it is not a very profitable to have this system here standing around, so I am wondering if there is anybody or any lab who might be interested in purchasing this system. It is installed on a HP Computer with a hardware dongle and we would like sell the complete system for a fair price for the PC and the licences of both applications. So, if there is anybody here in this community who is interested, please contact me personally. I can offer you more information about the PC and the software version, etc. and we can also discuss a possible price. With best regards Michael ************************************************************************************************ MORPHISTO Evolutionsforschung und Anwendung GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From michael.gudo at morphisto.de Wed Oct 18 07:38:37 2017 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Wed, 18 Oct 2017 14:38:37 +0200 Subject: [Histonet] Unused TissueFaxs Analysis-System (TissueQuest & HistQuest) Message-ID: Hello Histonetters, I am not sure if this question reaches the right persons here, but I try to place my question here: We have purchased a complete TissueFaxs-Akquisition and Analysis-System from TissueGnostics a few years ago for a research project. However, the project is finished for about 1 or 2 years, and now the Analysis System (TissueQuest and HistoQuest) will be not used here any more. I think it is not a very profitable to have this system here standing around, so I am wondering if there is anybody or any lab who might be interested in purchasing this system. Here are the information about the two Applications: http://www.tissuegnostics.com/en/products/analysing-software/histoquest http://www.tissuegnostics.com/en/products/analysing-software/tissuequest It is installed on a HP Computer with a hardware dongle and we would like sell the complete system for a fair price for the PC and the licences of both applications. So, if there is anybody here in this community who is interested, please contact me personally. I can offer you more information about the PC and the software version, etc. and we can also discuss a possible price. With best regards Michael ************************************************************************************************ MORPHISTO Evolutionsforschung und Anwendung GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From lindsey.s.fairbourn at aruplab.com Wed Oct 18 08:56:45 2017 From: lindsey.s.fairbourn at aruplab.com (Fairbourn, Lindsey S.) Date: Wed, 18 Oct 2017 13:56:45 +0000 Subject: [Histonet] QIHC Study Materials In-Reply-To: References: <8d119402cfe24e149dab8c664e3bae67@aruplab.com> Message-ID: <0e4c633736164883b9ffaa3d01f539b4@aruplab.com> Thank you Haley and Jay, we will check them out! From: Haley Huggins [mailto:hhuggins at novalabs.co] Sent: Tuesday, October 17, 2017 9:38 AM To: Jay Lundgren Cc: Fairbourn, Lindsey S.; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] QIHC Study Materials I sent to Lindsey in a private email which I thought I had hit reply all that Michigan Society of Histotechnology also has a study guide for the IHC, but you are right, Jay, that the Dako IHC manual is your best study guide. Haley Huggins, HT (ASCP)cm Technical Lab Supervisor 1050 Las Tablas Rd, Suite 14 Templeton, CA 93465 Office: 877-230-1518 On Tue, Oct 17, 2017 at 2:32 AM, Jay Lundgren via Histonet > wrote: The free one from Dako. > Virus-free. www.avg.com > <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> On Mon, Oct 16, 2017 at 12:28 PM, Fairbourn, Lindsey S. via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello Histonet, > > What is the best textbook to use to study for the QIHC exam? > > Thanks for your help! > Lindsey > > ------------------------------------------------------------------- > The information transmitted by this e-mail and any included > attachments are from ARUP Laboratories and are intended only for the > recipient. The information contained in this message is confidential > and may constitute inside or non-public information under > international, federal, or state securities laws, or protected health > information and is intended only for the use of the recipient. > Unauthorized forwarding, printing, copying, distributing, or use of > such information is strictly prohibited and may be unlawful. If you > are not the intended recipient, please promptly delete this e-mail > and notify the sender of the delivery error or you may call ARUP > Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 > (800) 522-2787 ext. 2100 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 From LBUSTAMANTE at cvm.tamu.edu Wed Oct 18 14:02:11 2017 From: LBUSTAMANTE at cvm.tamu.edu (Bustamante, Lin) Date: Wed, 18 Oct 2017 19:02:11 +0000 Subject: [Histonet] GI samples - Grossing Checklist Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC901D0BD50B7@CVMMB02.cvm.tamu.edu> We are in the process of training a technician for GI grossing . If you have a checklist for this training and willing to share, I will appreciate very much your help. Thank you. Lin. Lin S. Bustamante, BSc, HT(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone (979)845-3177 Fax (979)458-3499 From LBUSTAMANTE at cvm.tamu.edu Wed Oct 18 14:04:07 2017 From: LBUSTAMANTE at cvm.tamu.edu (Bustamante, Lin) Date: Wed, 18 Oct 2017 19:04:07 +0000 Subject: [Histonet] Leica Microtome RM2255 Message-ID: <94B6DC15AAF2F046BF847D4C1CA9AAC901D0BD50C7@CVMMB02.cvm.tamu.edu> We are looking for a Leica RM2255 automated microtome gently used or a demo. Thank you. Lin. Lin S. Bustamante, BSc, HT(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas A&M University College Station, Texas 77843-4458 Phone (979)845-3177 Fax (979)458-3499 From TNMayer at mdanderson.org Thu Oct 19 16:46:04 2017 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Thu, 19 Oct 2017 21:46:04 +0000 Subject: [Histonet] H&E stain protocols Message-ID: <47E9B2C01DDDD94881EACD2DC44EBC883A0275AB@D1PWPEXMBX08.mdanderson.edu> We have a few questions to ask: In your H&E protocols, what is your set up after eosin: 70, 95, 100, 100, xylene x 3or 95, 100, 100, 100, xylene x 3, or another variation? What brand of hematoxylin do you use? Eosin? Do you filter daily or at all? Eosin? How often do you change the hematoxylin? Eosin? Do you start with manufacturer suggestions for filtering and changing? Protocols? Carson says one thing, Bancroft something else, and the manufacturer different. I was taught 80, eosin, 70, 95, 100, 100, xylene x 3. We filtered on Wednesday, and changed and filtered on Monday. This was homemade dyes though. When we switched to Richard Allan we no longer filtered when we changed and then only when a sheen showed up, usually Monday's. The reason was Richard Allan does not require filtering. Thanks, Toysha The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From mverdu at histopat.es Fri Oct 20 01:22:47 2017 From: mverdu at histopat.es (=?UTF-8?Q?Montse_Verd=C3=BA?=) Date: Fri, 20 Oct 2017 08:22:47 +0200 Subject: [Histonet] Fwd: Tissue-Tek VIP 6 AI Vacuum Infiltration Processor In-Reply-To: References: Message-ID: Dear histonetters, Does anyone experience with the Tissue-Tek VIP 6 AI vacuum infiltration procesor? How often do you experience problems with these instruments that you need to have service for them? What kind of problems are they? Thank you in advance! Montse Verd? Histopat laboratoris Tel +34932033000 <+34%20932%2003%2030%2000> Biopat. Biopatologia Molecular Tel+34932030837 <+34%20932%2003%2008%2037> mverdu at histopat.es Aquest missatge i els seus annexos estan adre?ats exclusivament a la persona o entitat que figura com a destinatari i poden contenir dades i/o informaci? confidencial, sotmeses a secret professional o la divulgaci? de les quals estigui prohibida en virtut de la legislaci? vigent. Tota distribuci?, divulgaci? o reproducci? de aquesta comunicaci? per part de persones o entitats diferents del destinatari est? prohibida. Si ha rebut aquest missatge per error, si-us-plau, contacti amb la persona que figura com a remitent i procedeixi a la seva eliminaci?. Este mensaje y sus anexos van dirigidos exclusivamente a la persona o entidad que figura como destinatario, y pueden contener datos y/o informaci?n confidencial, sometidos a secreto profesional o cuya divulgaci?n est? prohibida en virtud de la legislaci?n vigente. Toda distribuci?n, divulgaci?n o reproducci?n de esta comunicaci?n por parte de personas o entidades distintas al destinatario est? prohibida. Si ha recibido este mensaje por error, por favor, contacte con la persona que figura como remitente y proceda a su eliminaci?n. This message and its attachments are addressed exclusively to the person or entity indicated as its named addressee, and may contain data and/or confidential information, subject to professional secret or whose disclosure is prohibited by virtue of current legislation in force. Any distribution, disclosure or reproduction of this type by persons or entities other than the addressee is strictly prohibited. If you have received this message by error, please contact the person indicated as its sender and delete it. From mverdu at histopat.es Fri Oct 20 01:23:39 2017 From: mverdu at histopat.es (=?UTF-8?Q?Montse_Verd=C3=BA?=) Date: Fri, 20 Oct 2017 08:23:39 +0200 Subject: [Histonet] Fwd: coverslipper Dako question In-Reply-To: <73b613a754e9205a41d06905f887e81e@mail.gmail.com> References: <73b613a754e9205a41d06905f887e81e@mail.gmail.com> Message-ID: Dear histonetters, Does anyone experience with the Coverslipper Dako? How often do you experience problems with these instruments that you need to have service for them? What kind of problems are they? In our case the drawer is not securely attached, the magnet is not securely attached to the equipment so the basket collides with the drawer and breaks the slides. Does anyone else have this same problem? Thank you in advance! Montse Verd? Histopat laboratoris Tel +34932033000 <+34%20932%2003%2030%2000> Biopat. Biopatologia Molecular Tel+34932030837 <+34%20932%2003%2008%2037> mverdu at histopat.es Aquest missatge i els seus annexos estan adre?ats exclusivament a la persona o entitat que figura com a destinatari i poden contenir dades i/o informaci? confidencial, sotmeses a secret professional o la divulgaci? de les quals estigui prohibida en virtut de la legislaci? vigent. Tota distribuci?, divulgaci? o reproducci? de aquesta comunicaci? per part de persones o entitats diferents del destinatari est? prohibida. Si ha rebut aquest missatge per error, si-us-plau, contacti amb la persona que figura com a remitent i procedeixi a la seva eliminaci?. Este mensaje y sus anexos van dirigidos exclusivamente a la persona o entidad que figura como destinatario, y pueden contener datos y/o informaci?n confidencial, sometidos a secreto profesional o cuya divulgaci?n est? prohibida en virtud de la legislaci?n vigente. Toda distribuci?n, divulgaci?n o reproducci?n de esta comunicaci?n por parte de personas o entidades distintas al destinatario est? prohibida. Si ha recibido este mensaje por error, por favor, contacte con la persona que figura como remitente y proceda a su eliminaci?n. This message and its attachments are addressed exclusively to the person or entity indicated as its named addressee, and may contain data and/or confidential information, subject to professional secret or whose disclosure is prohibited by virtue of current legislation in force. Any distribution, disclosure or reproduction of this type by persons or entities other than the addressee is strictly prohibited. If you have received this message by error, please contact the person indicated as its sender and delete it. From michael.gudo at morphisto.de Fri Oct 20 03:13:53 2017 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Fri, 20 Oct 2017 10:13:53 +0200 Subject: [Histonet] H&E stain protocols In-Reply-To: <47E9B2C01DDDD94881EACD2DC44EBC883A0275AB@D1PWPEXMBX08.mdanderson.edu> References: <47E9B2C01DDDD94881EACD2DC44EBC883A0275AB@D1PWPEXMBX08.mdanderson.edu> Message-ID: Dear Toysha, eosin stainings are soluble in lower alkohols, therefore the traditional procedure is to go shortly into 80 percent, just to remove surplus eosin but not more and then go directly in 96 (or 95) and 100 % alkohol. In our lab we use 96 % ethanol and then 2x 100 pesent 2-propanol. Filtering of Eosin is useful if you stain many slides and the pH-value will change during staining. Since there are many different eosins etsablished in the labs (water-based eosins in 0.2, 0.5, 1.0 and 2.0 %, or ethanol based eosins in 0.2, 0.5, 1.0 or 2.0 % and these also with or without additional acetic acid to have pH values between 4.0 or 6.0), the protocols differ much from each other. In our lab we use 1.0 % Eosin, in water or ethanol, and we change the solution (500 ml) after staining ~ 500 slides, and we do not filter, however, we check the pH-value each morning, and if this value is depart from 4,0 or 6,0 (depending on the results we want) we adjust the pH value with acetic acid and we filter the solution. We use the Eosin which the chemical part of our company produces for the european market. So if you are interested, just contact me personally, we are now also able to deliver our products overseas. With best regards Michael > Am 19.10.2017 um 23:46 schrieb Mayer,Toysha N via Histonet : > > We have a few questions to ask: > In your H&E protocols, what is your set up after eosin: 70, 95, 100, 100, xylene x 3or 95, 100, 100, 100, xylene x 3, or another variation? > What brand of hematoxylin do you use? Eosin? > Do you filter daily or at all? Eosin? > How often do you change the hematoxylin? Eosin? > Do you start with manufacturer suggestions for filtering and changing? Protocols? > Carson says one thing, Bancroft something else, and the manufacturer different. > I was taught 80, eosin, 70, 95, 100, 100, xylene x 3. > We filtered on Wednesday, and changed and filtered on Monday. > This was homemade dyes though. > When we switched to Richard Allan we no longer filtered when we changed and then only when a sheen showed up, usually Monday's. The reason was Richard Allan does not require filtering. > > Thanks, > Toysha > > > The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO Evolutionsforschung und Anwendung GmbH PD Dr. phil. nat. Michael Gudo Weism?llerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From redrose297 at gmail.com Fri Oct 20 06:47:07 2017 From: redrose297 at gmail.com (warda hassan) Date: Fri, 20 Oct 2017 11:47:07 +0000 Subject: [Histonet] Requesting feedback on few instruments Message-ID: Dear Histo-net-Group Hope you all are doing good, we are looking to purchase Couple of new instruments in our laboratory with an worked of 60 surgical specimens per day including 3 breast mastectomy and colons. The instruments list are 1-Dako routine Stainer with cover sliper 2-Dako Special stainer 3-Roche Special Stainer I would request if anyone of you had experienced using these and your valuable feedback will be of immense help. Thank you Happy weekend Royal Hospital-Oman From carl.hobbs at kcl.ac.uk Sun Oct 22 12:03:42 2017 From: carl.hobbs at kcl.ac.uk (Hobbs, Carl) Date: Sun, 22 Oct 2017 17:03:42 +0000 Subject: [Histonet] logging in to Histonet Images Message-ID: Hi My apologies for using this way....I don't know how else. I have a new PC ( lost all my links) and forgot how to log in/haven't got the link , then upload images/edit them All I can do is upload images. I'd be most grateful for link so I can edit my posts. Thank you! carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 From rhworkman at uro.com Mon Oct 23 15:24:24 2017 From: rhworkman at uro.com (Renee H. Workman) Date: Mon, 23 Oct 2017 20:24:24 +0000 Subject: [Histonet] Re-attaching slide film to slide Message-ID: Hey Histonetter, How can I re-attach the film with the tissue back to the blank slide? Have some older slides and the slide film has popped off. Renee H. Workman Histology Supervisor Virginia Urology 9105 Stony Point Drive Richmond, VA 23235 W: 804-527-1316 | F: 804-270-0917 rhworkman at uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. From diane.satterfield at duke.edu Mon Oct 23 15:28:08 2017 From: diane.satterfield at duke.edu (Diane Satterfield) Date: Mon, 23 Oct 2017 20:28:08 +0000 Subject: [Histonet] H&E LFB Message-ID: Does anyone have a protocol/SOP for doing H&E LFB staining they would be willing to share with me? Diane L. Satterfield, BS Manager Brain Tumor BioRepository Research Program Leader Duke University Medical Center Brain Tumor Center Biorepository and Database From Carrie.Minikus at unitypoint.org Tue Oct 24 07:50:58 2017 From: Carrie.Minikus at unitypoint.org (Minikus, Carrie A.) Date: Tue, 24 Oct 2017 12:50:58 +0000 Subject: [Histonet] Thermo HM525 NX Message-ID: <40DFB208CC287B4091BE635AC60402C063A2E45B@TDCEXCHXM005.ihs.org> For anyone who uses the Thermo HM525 NX cryostat, how do you clean out the shavings that fall behind the stage? I know you can remove some of the pieces to try to clean out by hand but some of our staff do no feel comfortable with sticking their hands down there with the wires. We use a small vacuum cleaner to suck the shavings out at our satellite lab but we only do frozen on skins out there and the issue at hand with using a vacuum at our hospital setting is that we cut lung tissue on it and don't want to risk the potential chance of using a vacuum to suck up TB or any other lung diseases. We were wondering if there was just a special filter we could use? Any suggestions would be greatly appreciated! Carrie Minikus, MLS(ASCP)CM, HTL(ASCP)CM Histology Manager This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. ?? 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From CindiS at unionhospital.org Tue Oct 24 08:20:01 2017 From: CindiS at unionhospital.org (Cindi Seevers) Date: Tue, 24 Oct 2017 13:20:01 +0000 Subject: [Histonet] Cytology slides read workload limits for Ohio Message-ID: <94562B26E65415478076251B7EB96E330B0D9C@UHEXG-8.unionhospital.org> Is anyone able to help me out with the current standards for Ohio's cytology slides read workload limits? I am working on CAP inspection updates and we do not have a cytotech at our hospital, so I am in charge of cytology and histology manuals. I need to know if Ohio's standards are more stringent than the CLIA standards. Current CLIA standards state that an individual evaluating cytology preparations by manual microscopic technique must examine no more than 100 slides (gyn and non-gyn or both) in 24 hours. Is this the same standard for Ohio? I have emailed the Ohio cytology society 3 times and I have emailed a lady that is supposed to be in the Ohio cytology society group and have not received any response. Any help would be greatly appreciated!! Thanks in advance!!! Cindi Seevers HT, ASCP Histology Supervisor Union Hospital Dover, OH This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery. From lblazek at digestivespecialists.com Tue Oct 24 10:29:54 2017 From: lblazek at digestivespecialists.com (Blazek, Linda) Date: Tue, 24 Oct 2017 11:29:54 -0400 Subject: [Histonet] counters Message-ID: <5A2BD13465E061429D6455C8D6B40E392833CE046B@IBMB7Exchange.digestivespecialists.com> Good morning. Does anyone have a picture of a curved counter top for use with the microtome? I thought I had one but can't seem to find it. Thanks! Linda Blazek HT (ASCP) Pathology Lab Manager GI Pathology of Dayton Digestive Specialists, Inc Phone: (937) 396-2623 Email: lblazek at digestivespecialists.com From Nancy_Schmitt at pa-ucl.com Tue Oct 24 12:16:24 2017 From: Nancy_Schmitt at pa-ucl.com (Nancy Schmitt) Date: Tue, 24 Oct 2017 17:16:24 +0000 Subject: [Histonet] validation Message-ID: Good Day! When performing validation on IHC, we use 20 positives and 20 negative cases. When testing for negative, can the normal tissue surrounding the tumor be counted as negative? Does the negative tissue need to be tumor that is negative for the AB or just normal tissue? Thank you for your consideration, Nancy Dubuque, IA Ph. 563-589-9810 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From twebster at CRH.org Tue Oct 24 12:34:36 2017 From: twebster at CRH.org (Webster, Thomas S.) Date: Tue, 24 Oct 2017 17:34:36 +0000 Subject: [Histonet] Cytology slides read workload limits for Ohio Message-ID: <7207186ED68FB542803CAF1CE6E82FF8978EC297@exmb1.crh.org> Ohio's standards aren't more stringent. The only states, I know of, that differ from the 100 slides in 24 hours limit is New York and California. CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201 From teri.johnson at navigatebp.com Tue Oct 24 16:53:56 2017 From: teri.johnson at navigatebp.com (Johnson, Teri) Date: Tue, 24 Oct 2017 21:53:56 +0000 Subject: [Histonet] Tracking lot numbers Message-ID: <5f44baa19af54f7f8f56466b5e42d19a@BN1PR62MB038.023d.mgd.msft.net> Hi Histonetters, I need to find out what labs are doing for best practices to track reagent lot numbers in their tissue processor as they rotate reagents. We currently have a preparation log where we track the lot numbers, but by the time we do a rotation we may be using a different lot. As always, thanks for your help and input. Teri Johnson, HT(ASCP)QIHC Manager, Clinical Trial Testing T +1 760 516 5954 teri.johnson at navigatebp.com Navigate BioPharma, Inc. A Novartis Subsidiary 1890 Rutherford Rd. Carlsbad, CA? 92008 USA From liz at premierlab.com Tue Oct 24 17:33:06 2017 From: liz at premierlab.com (Liz Chlipala) Date: Tue, 24 Oct 2017 22:33:06 +0000 Subject: [Histonet] Tracking lot numbers In-Reply-To: <5f44baa19af54f7f8f56466b5e42d19a@BN1PR62MB038.023d.mgd.msft.net> References: <5f44baa19af54f7f8f56466b5e42d19a@BN1PR62MB038.023d.mgd.msft.net> Message-ID: Teri When we change the tissue processor or H&E stainer instead of just recording a "check mark" that the solution has been changed we record the lot numbers on the form instead. We also have a from that is posted above the tissue processor that we fill in with dry erase the current lot numbers and expiration dates of the solutions. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: Johnson, Teri via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Tuesday, October 24, 2017 3:54 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Tracking lot numbers Hi Histonetters, I need to find out what labs are doing for best practices to track reagent lot numbers in their tissue processor as they rotate reagents. We currently have a preparation log where we track the lot numbers, but by the time we do a rotation we may be using a different lot. As always, thanks for your help and input. Teri Johnson, HT(ASCP)QIHC Manager, Clinical Trial Testing T +1 760 516 5954 teri.johnson at navigatebp.com Navigate BioPharma, Inc. A Novartis Subsidiary 1890 Rutherford Rd. Carlsbad, CA 92008 USA _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From criley at dpspa.com Wed Oct 25 07:46:42 2017 From: criley at dpspa.com (Charles Riley) Date: Wed, 25 Oct 2017 08:46:42 -0400 Subject: [Histonet] Cryostat question Message-ID: Does anyone out there use the Avantik QS12 cryostats? We have a machine that is only a year and a half old and have had to get the Peltier system (the cryobar) replaced 4 times. Is anyone else having this problem consistently? Sent from my iPhone From pmdooley27 at gmail.com Wed Oct 25 09:12:28 2017 From: pmdooley27 at gmail.com (Patrick Dooley) Date: Wed, 25 Oct 2017 10:12:28 -0400 Subject: [Histonet] H&E LFB Message-ID: Here's my lab's SOP for an LHE. 7uM sections. Hope this helps! 1. Deparaffinize and hydrate to absolute ethanol. 2. Luxol Fast Blue 60? C oven 1hr. 3. Cool to room temperature. 4. Hydrate to water. 5. 2 - 3 dips reducer. The fresher the reducer the less number of dips you need. You'll need to look under the microscope to determine an adequate differentiation; the best is when you can still clearly see the axonal processes extending into the neocortex but with not much staining in the grey matter at all. 6. Rinse in tap water until water is clear. 7. Harris Hematoxlyn 10 min. 8. Rinse in tap water until water is clear. 9. Differentiate in acid alcohol. This usually takes 1-2 dips. Again, look under the microscope to detemine a sufficient stopping point to differentiation. 10. Rinse in tap water until water is clear. 11. Eosin for 5 - 7 minutes. 12. Rinse in tap water until water is clear. 13. Dehydrate to absolute ethanol. This step adequately reduces the background eosin in our lab, but you may need to leave/dip more in 95% ethanol as that will differentiate it the best. 14. 10 dips in two separate changes of xylene. 15. Coverslip. Solutions are as follows: Luxol Fast Blue 10 g Luxol Fast Blue MBS 1 liter 95% ethanol 0.5 ml glacial acetic acid Reducer 10 g hydroquinone 50 g Na2SO4 1 liter distilled water Acid Alcohol 10 ml conc. HOAc 1 liter 70% ethanol Eosin 0.5% aqueous Eosin Y when first used, add 1 ml glacial HOAc / 400 ml eosin Patrick Dooley, HTL(ASCP) Research Tech I / Tissue Bank Coordinator MGH Alzheimer?s Disease Resource Center CNY 114 Rm. 2650 617-643-6994(office) From edmartin26 at gmail.com Wed Oct 25 15:50:16 2017 From: edmartin26 at gmail.com (Eddie Martin) Date: Wed, 25 Oct 2017 16:50:16 -0400 Subject: [Histonet] validation inquiry regarding negative tissue Message-ID: <3ED691D8-41D2-426D-B935-FFBBFE885B8F@gmail.com> Hi Nancy, The use of negative internal control tissue on your antibody testing for negative cases would have to be determined by your medical director/chief pathologist as acceptable. You may also use Lipoma tissue as a negative control tissue. It works as a negative control for just about every antibody I can think about. There may also be a few antibodies that would have false positive staining with a lipoma, but a pathologist could read through that stuff. Hope this helps! Kind regards, Eddie M. Edward M. Martin, HTL, HT(ASCP), QIHC(ASCP) Lead Hematopathology Medical Technologist The National Institutes of Health and IHC Histotechnologist III The Joint Pathology Center Walter Reed NMMC Bethesda, MD > On Oct 24, 2017, at 1:16 PM, Nancy Schmitt wrote: > > Good Day! > When performing validation on IHC, we use 20 positives and 20 negative cases. > > When testing for negative, can the normal tissue surrounding the tumor be counted as negative? > > Does the negative tissue need to be tumor that is negative for the AB or just normal tissue? > > Thank you for your consideration, > > Nancy > Dubuque, IA > Ph. 563-589-9810 > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > From tony.henwood at health.nsw.gov.au Wed Oct 25 18:57:24 2017 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Wed, 25 Oct 2017 23:57:24 +0000 Subject: [Histonet] validation In-Reply-To: References: Message-ID: <0237449DE79DBC45B686AB82CDCD16FF9558E884@SVDCMBX-MEX008.nswhealth.net> Immunohistochemistry validation is much more than simply 20 positives and 20 negative cases, though this will allow you to meet most accreditation requirements. Validation is a multi step process: 1. Optimisation: following a thorough literature review (what should and should not stain, clones used (any difference), recommended assay conditions (eg antigen retrieval type), choose an appropriate control to confirm staining conditions and perform an optimum titre. 2. Validation: based on the literature review, choose appropriate known (or expected) positives and negative cases that the pathologists would entertain in the differential diagnosis that should be negative. Take Prostate Specific Antigen as an example, include known prostatic carcinomas with a mix of well, moderately and poorly differentiated prostatic carcinomas and include negative tumours that should not express PSA (eg melanomas, lymphomas, colonic and lung carcinomas). This is a scientific approach that inspires confidence in the usefulness of the test. 3. Another task that we find extremely useful is on-going Verification, where we compare staining results with final diagnosis of cases immunostained for the marker. Food for thought? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Nancy Schmitt via Histonet [mailto:histonet at lists.utsouthwestern.edu] Sent: Wednesday, 25 October 2017 4:16 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] validation Good Day! When performing validation on IHC, we use 20 positives and 20 negative cases. When testing for negative, can the normal tissue surrounding the tumor be counted as negative? Does the negative tissue need to be tumor that is negative for the AB or just normal tissue? Thank you for your consideration, Nancy Dubuque, IA Ph. 563-589-9810 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. From NIngrao at KaleidaHealth.Org Thu Oct 26 08:31:58 2017 From: NIngrao at KaleidaHealth.Org (Ingrao, Nicholas) Date: Thu, 26 Oct 2017 13:31:58 +0000 Subject: [Histonet] Uromodulin IHC Message-ID: Looking for a reference lab that performs Uromodulin IHC, preference to NYS permitted lab. Thanks! Nick Ingrao, MBA, MT(ASCP) Manager, Anatomic Pathology Kaleida Health: Buffalo General Medical Center 100 High St Buffalo, NY 14203 Office:(716) 859-2028 Pager: (716) 642-0232 Fax: (716) 859-2393 [Leading with CARE] The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida Health???s Technology Assistance Center at (716) 859-7777. From relia1 at earthlink.net Thu Oct 26 10:56:33 2017 From: relia1 at earthlink.net (Pam Barker) Date: Thu, 26 Oct 2017 11:56:33 -0400 Subject: [Histonet] RELIA Special Job Alert for Managers and Supervisors Exciting New Opportunities and More on the Way 10-26-17 Message-ID: <08ca01d34e72$fefc1d10$fcf45730$@earthlink.net> Hello Histonetters, I have several exciting opportunities for experienced Managers, and Supervisors in hospital and private lab environments in several locations throughout the US. These are some of the premier employers in the United States. The positions are of course full time and permanent. My clients offer excellent compensation, benefits and relocation assistance. Here are my leadership positions: AP Manager - Charlotte, NC Lab Manager - Nashville, TN Histology Supervisor - Birmingham, AL -Brand New Lab!! Mohs Histology Supervisor - Naples, FL Histology Lab Manager - Salem, VA Team Lead - Histology - Nashville, TN Lead Histology Tech - Birmingham, AL - Brand New Lab! I anticipate multiple management openings with many exciting opportunities for talented managers and supervisor to be coming available nationwide after the holidays as well. If you are interested in looking into a change in another area of the country please let me know. That way I can keep you apprised of management opportunities in that area. Also if you would like more information or know of someone else who might be interested, please contact me! If you would like to chat right away please call my cell at 407-353-5070 OR I can be reached at relia1 at earthlink.net or 866-607-3542. I am available to discuss the opportunities and what you are looking for at your convenience including after hours. Thanks-Pam There are a lot of recruiters out there right now trying to work with histology professionals and I appreciate your support and respect your needs. Remember I offer over 30 years of experience as a recruiter and for over 15 years I have dedicated my practice solely to placing histology professionals like you. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From c.tague at Pathologyarts.com Thu Oct 26 12:02:40 2017 From: c.tague at Pathologyarts.com (Curt) Date: Thu, 26 Oct 2017 17:02:40 +0000 Subject: [Histonet] changing carbon filters Message-ID: <9C8F910F72893643B3C3793C3D67132B68ADDBE2@PATHOLOGYSERVER.pathologyarts.local> How often is everyone changing the carbon filters in their tissue processors and stainer, maybe even the thermo-shandon hood filters too? Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. From LRaff at uropartners.com Fri Oct 27 10:32:25 2017 From: LRaff at uropartners.com (Lester Raff MD) Date: Fri, 27 Oct 2017 15:32:25 +0000 Subject: [Histonet] Friday Fun--Lab Related Blog Post Message-ID: <6347C6D2B080534F9B5C2B08436DCFAF116BA75B@COLOEXCH01.uropartners.local> It was cytology, but could be histology just as easily! http://www.chicagonow.com/downsize-maybe/2017/10/the-safety-engineers-reply-will-make-you-wonder/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From tbraud at holyredeemer.com Fri Oct 27 14:32:49 2017 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 27 Oct 2017 19:32:49 +0000 Subject: [Histonet] pertinent to the blog Message-ID: <48E053DDF6CE074DB6A7414BA05403F84CE98FC0@HRHEX03-HOS.holyredeemer.local> Actually, the verification of instrument performance is a CAP All Common Checklist Requirement and should already be a part of laboratory procedure. COM.30550 Instrument/Equipment Performance Verification Phase II The performance of all instruments and equipment is verified prior to initial use, after major maintenance or service, and after relocation to ensure that they run according to expectations. NOTE: Instrument/equipment performance verification (NOT to be confused with validation or verification of the test method performance specifications) includes processes to verify that the instruments and equipment perform according to expectations for the intended use and within defined tolerance limits. If instruments or equipment are moved, the laboratory must perform appropriate function checks to ensure that they were not adversely affected by the relocation process or changes due to the new environment. This does not apply to portable equipment used following the manufacturer's instructions. Evidence of Compliance: ? Written procedure to verify proper functioning of instruments and equipment prior to initial use, after major maintenance or service, and after relocation AND ? Records of appropriate function checks Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal -----Original Message----- From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Friday, October 27, 2017 1:00 PM Message: 2 Date: Fri, 27 Oct 2017 15:32:25 +0000 From: Lester Raff MD It was cytology, but could be histology just as easily! http://www.chicagonow.com/downsize-maybe/2017/10/the-safety-engineers-reply-will-make-you-wonder/ Lester J. Raff, MD MBA UroPartners Medical Director Of Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, Il 60154 Tel: 708-486-0076 Fax: 708-492-0203 From victor_tobias at comcast.net Sat Oct 28 17:19:19 2017 From: victor_tobias at comcast.net (Victor) Date: Sat, 28 Oct 2017 15:19:19 -0700 Subject: [Histonet] Ann Preece Manual Message-ID: I have a copy, 1st edition of A Manual for Histologic Technicians by Ann Preece. On the inside cover it says Wyoming Valley Hospital Laboratory 1962. I don?t recall how I got it, but I never worked for the facility. Great for a new student or Histology program. I?ll pay shipping to whomever is interested. Victor Sent from Mail for Windows 10 From bakevictoria at gmail.com Sat Oct 28 17:41:29 2017 From: bakevictoria at gmail.com (Victoria Baker) Date: Sat, 28 Oct 2017 18:41:29 -0400 Subject: [Histonet] Ann Preece Manual In-Reply-To: References: Message-ID: We currently have a student in our lab. She took her classes through Broome community college were the program is in its infancy. I think she would benefit from this book. I can give you contact information off line and it would be a hospital address. Vikki Baker UHS Wilson Hospital On Oct 28, 2017 6:31 PM, "Victor via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > I have a copy, 1st edition of A Manual for Histologic Technicians by Ann > Preece. On the inside cover it says Wyoming Valley Hospital Laboratory > 1962. I don?t recall how I got it, but I never worked for the facility. > Great for a new student or Histology program. I?ll pay shipping to whomever > is interested. > > Victor > > Sent from Mail for Windows 10 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bakevictoria at gmail.com Sat Oct 28 20:15:38 2017 From: bakevictoria at gmail.com (Victoria Baker) Date: Sat, 28 Oct 2017 21:15:38 -0400 Subject: [Histonet] Ann Preece Manual In-Reply-To: References: Message-ID: We currently have a student in our lab. She took her classes through Broome community college were the program is in its infancy. I think she would benefit from this book. I can give you contact information off line and it would be a hospital address. Vikki Baker UHS Wilson Hospital On Oct 28, 2017 6:31 PM, "Victor via Histonet" < histonet at lists.utsouthwestern.edu> wrote: > I have a copy, 1st edition of A Manual for Histologic Technicians by Ann > Preece. On the inside cover it says Wyoming Valley Hospital Laboratory > 1962. I don?t recall how I got it, but I never worked for the facility. > Great for a new student or Histology program. I?ll pay shipping to whomever > is interested. > > Victor > > Sent from Mail for Windows 10 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From victor_tobias at comcast.net Sat Oct 28 21:20:39 2017 From: victor_tobias at comcast.net (victor_tobias at comcast.net) Date: Sun, 29 Oct 2017 02:20:39 +0000 (UTC) Subject: [Histonet] Ann Preece Manual In-Reply-To: References: Message-ID: <1442588018.12994478.1509243639513.JavaMail.zimbra@comcast.net> Thank you to all who showed an interest in the book. The book has been spoken for. Victor Sent from XFINITY Connect Mobile App ------ Original Message ------ From: Victor via Histonet To: histonet at lists.utsouthwestern.edu Sent: October 28, 2017 at 3:19 PM Subject: [Histonet] Ann Preece Manual I have a copy, 1st edition of A Manual for Histologic Technicians by Ann Preece. On the inside cover it says Wyoming Valley Hospital Laboratory 1962. I don?t recall how I got it, but I never worked for the facility. Great for a new student or Histology program. I?ll pay shipping to whomever is interested. Victor Sent from Mail for Windows 10 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Sun Oct 29 21:07:11 2017 From: rsrichmond at gmail.com (Bob Richmond) Date: Sun, 29 Oct 2017 22:07:11 -0400 Subject: [Histonet] Histonet Digest, Vol 167, Issue 22 In-Reply-To: References: Message-ID: The old Samurai Pathologist has a copy of Ann Preece's book. I was contemptuous of it as an arrogant young pathologist of 28, deeply respectful of it as an arrogant old pathologist of 78. But it's a reproach that a fifty year old book has not been superseded. Bob Richmond Samurai Pathologist Maryville TN On Sun, Oct 29, 2017 at 1:00 PM, wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Ann Preece Manual (Victor) > 2. Re: Ann Preece Manual (Victoria Baker) > 3. Re: Ann Preece Manual (Victoria Baker) > 4. Re: Ann Preece Manual (victor_tobias at comcast.net) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 28 Oct 2017 15:19:19 -0700 > From: Victor > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Ann Preece Manual > Message-ID: > > Content-Type: text/plain; charset="utf-8" > > I have a copy, 1st edition of A Manual for Histologic Technicians by Ann > Preece. On the inside cover it says Wyoming Valley Hospital Laboratory > 1962. I don?t recall how I got it, but I never worked for the facility. > Great for a new student or Histology program. I?ll pay shipping to whomever > is interested. > > Victor > > Sent from Mail for Windows 10 > > > > ------------------------------ > > Message: 2 > Date: Sat, 28 Oct 2017 18:41:29 -0400 > From: Victoria Baker > To: Victor > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Ann Preece Manual > Message-ID: > gmail.com> > Content-Type: text/plain; charset="UTF-8" > > We currently have a student in our lab. She took her classes through > Broome community college were the program is in its infancy. I think she > would benefit from this book. > > I can give you contact information off line and it would be a hospital > address. > > Vikki Baker > UHS Wilson Hospital > > > On Oct 28, 2017 6:31 PM, "Victor via Histonet" < > histonet at lists.utsouthwestern.edu> wrote: > > > I have a copy, 1st edition of A Manual for Histologic Technicians by Ann > > Preece. On the inside cover it says Wyoming Valley Hospital Laboratory > > 1962. I don?t recall how I got it, but I never worked for the facility. > > Great for a new student or Histology program. I?ll pay shipping to > whomever > > is interested. > > > > Victor > > > > Sent from Mail for Windows 10 > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 3 > Date: Sat, 28 Oct 2017 21:15:38 -0400 > From: Victoria Baker > To: Victor > Cc: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Ann Preece Manual > Message-ID: > WOVKeKiVYOqhw at mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > We currently have a student in our lab. She took her classes through > Broome community college were the program is in its infancy. I think she > would benefit from this book. > > I can give you contact information off line and it would be a hospital > address. > > Vikki Baker > UHS Wilson Hospital > > > On Oct 28, 2017 6:31 PM, "Victor via Histonet" < > histonet at lists.utsouthwestern.edu> wrote: > > > I have a copy, 1st edition of A Manual for Histologic Technicians by Ann > > Preece. On the inside cover it says Wyoming Valley Hospital Laboratory > > 1962. I don?t recall how I got it, but I never worked for the facility. > > Great for a new student or Histology program. I?ll pay shipping to > whomever > > is interested. > > > > Victor > > > > Sent from Mail for Windows 10 > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 4 > Date: Sun, 29 Oct 2017 02:20:39 +0000 (UTC) > From: victor_tobias at comcast.net > To: "" , Victor > > Subject: Re: [Histonet] Ann Preece Manual > Message-ID: > <1442588018.12994478.1509243639513.JavaMail.zimbra at comcast.net> > Content-Type: text/plain; charset="utf-8" > > Thank you to all who showed an interest in the book. The book has been > spoken for. > Victor > Sent from XFINITY Connect Mobile App > ------ Original Message ------ > From: Victor via Histonet > To: histonet at lists.utsouthwestern.edu > Sent: October 28, 2017 at 3:19 PM > Subject: [Histonet] Ann Preece Manual > I have a copy, 1st edition of A Manual for Histologic Technicians by > Ann Preece. On the inside cover it says Wyoming Valley Hospital > Laboratory 1962. I don?t recall how I got it, but I never worked for > the facility. Great for a new student or Histology program. I?ll pay > shipping to whomever is interested. > Victor > Sent from Mail for Windows 10 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 167, Issue 22 > ***************************************** > From bliven.laura at marshfieldclinic.org Mon Oct 30 14:14:05 2017 From: bliven.laura at marshfieldclinic.org (Bliven, Laura M) Date: Mon, 30 Oct 2017 19:14:05 +0000 Subject: [Histonet] Microdissection - Crystals in tissue Message-ID: Looking for a lab to perform microdissection on tissue to remove crystals for testing. Please advise. Thanks, Laura ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From rfisher0126 at msn.com Tue Oct 31 10:21:48 2017 From: rfisher0126 at msn.com (Renee Fisher) Date: Tue, 31 Oct 2017 15:21:48 +0000 Subject: [Histonet] Kappa & Lambda ISH Message-ID: Hello histonet, I am doing K&L ISH on BM and Lymphoma cases using the Leica Bond refine red detection. The BM are staining beautifully however, the lymph nodes are not staining at all in particular Follicular Lymphoma cases. Any suggestions? Thank you From relia1 at earthlink.net Tue Oct 31 10:41:58 2017 From: relia1 at earthlink.net (Pam Barker) Date: Tue, 31 Oct 2017 11:41:58 -0400 Subject: [Histonet] Happy Halloween! Message-ID: <1c8c01d3525e$c9b0b180$5d121480$@earthlink.net> Hi Histonetters, Happy Halloween!!! TRICK or TREAT!!!!! The TRICK is finding the right job opportunity for you. The TREAT is with my help it can be relatively painless. . I will help you with your resume, . Coach you through the interview and offer process . And refer you to positions based on the criteria you give me. All of the positions I work with are fulltime permanent positions with some of the best facilities Nationwide. My clients offer excellent compensation including competitive salaries, great benefits, relocation assistance/sign on bonuses. I have exciting opportunities in these areas! Management/Supervisor/Lead Tech: A.P. Manager Charlotte, NC Histology Supervisor Birmingham, AL - Brand New Lab! 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I will only represent you to jobs you tell me you are interested in looking into. Remember. It never hurts to keep an eye open, even if you are happy in your present job. p.s. How are you celebrating Halloween? Happy Halloween!!!!!!!!!!!! Pam - 866-607-3542 (866-60RELIA) Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia From greg.dobbin at gmail.com Tue Oct 31 12:15:00 2017 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Tue, 31 Oct 2017 14:15:00 -0300 Subject: [Histonet] Kappa & Lambda ISH Message-ID: Hello Renee, We do K & L by ISH on the Bond-III and BondMax with Refine detection without issue on any of the tissues we have run (GI, skin, BM, LN). We have a normal tonsil control section on every slide. Do you also run a positive control on every slide? How does it perform? Next step would be for me to share our protocol. Let me know if you would like me to send that to you. Cheers, Greg -- *Greg Dobbin, R.T.* Chief Technologist, Anatomic Pathology Dept. of Laboratory Medicine, Queen Elizabeth Hospital, P.O. Box 6600 Charlottetown, PE C1A 8T5 Phone: (902) 894-2337 Fax: (902) 894-2385 *Everything in moderation...even moderation itself**!*